PMC:5608921 / 30898-32297 JSONTXT

{"project":"2_test","denotations":[{"id":"28846079-11063126-79081961","span":{"begin":253,"end":255},"obj":"11063126"}],"text":"Statistic analysis\nAnimals were sorted only by genotype or treatment, and although exclusion or inclusion of an animal was not predetermined, some mice of the Tg(K6b-E6/E7) died during the experimental treatment (the majority died before 2 months of age26); it is important to mention, however, that the cause of death was never associated with the presence of tumors. Specific blinding or randomization method was not applied. The size of each experimental group was limited according with reproducibility and extent of difference; generally, small groups (3–4 independent individuals) were only considered for determination with an evident qualitative difference between groups. Fisher's exact test was used for the analysis of ear regeneration data; since there is a low correlation between a mouse and the regeneration efficiency of its ears, each ear was taken as an independent regeneration event. For other experiments, the t-Student test was performed when the distribution was normal, or the Mann–Whitney Rank-Sum Test when the data distribution was not normal. Since the intact and perforated ear sample for gene expression analysis derived from the same mouse, the paired t-Student test was used in this case. The results are shown as the mean±s.d. and the variance analysis was performed when two data sets were analyzed. We considered significant differences when the P-value was ≤0.05."}