PMC:5608921 / 26331-26991 JSONTXT

{"project":"2_test","denotations":[{"id":"28846079-18548112-79081960","span":{"begin":389,"end":391},"obj":"18548112"}],"text":"Hole closure assay\nExcisional 2 mm punches were made with a metal ear puncher on the center of ears of 6 weeks and 3-month-old mice. The hole diameter was measured 4 weeks after punching under a dissecting microscope. Formation of new hair follicles and cartilage was determined by visualizing them in samples stained with fast green and safranin after 24 h or 3 months of ear perforation.21 Ear regeneration efficiency was classified into four categories (gray scale in bar graphs) based on the following estimation:\nPercent of regeneration (Pr)=100−(100·non-regenerated area/area excised)\n\nwhere r1=measured diameter (mm)/2, and r2 =2 mm/2=1 mm\nTherefore,\n\nA"}