PMC:5590178 / 11689-12378 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/5590178","sourcedb":"PMC","sourceid":"5590178","source_url":"https://www.ncbi.nlm.nih.gov/pmc/5590178","text":"Zebrafish study\nWild-type (WT) zebrafish (AB strain) were obtained from the Zebrafish International Resource Center (Eugene, OR), maintained using standard procedures [29] and staged in hours post-fertilization (hpf) as per standard criteria [30]. One-cell stage zebrafish embryos were microinjected with GFAP expression constructs (50 pg), anesthetized at 30 hpf in 0.02% tricane, mounted with 3% methylcellulose and imaged with an LSM 510 CLM (Zeiss, Hamburg, Germany). Z-series of images (15 images; interval thickness: 1.0 μm) were collected and presented as a stacking image. Resulting images were assembled using Adobe Photoshop (San Jose, CA), and aggregations were counted blindly.","divisions":[{"label":"title","span":{"begin":0,"end":15}}],"tracks":[{"project":"2_test","denotations":[{"id":"28882119-8589427-12765642","span":{"begin":243,"end":245},"obj":"8589427"}],"attributes":[{"subj":"28882119-8589427-12765642","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec93cf","default":true}]}]}}