PMC:5512188 / 5963-6637 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/5512188","sourcedb":"PMC","sourceid":"5512188","source_url":"https://www.ncbi.nlm.nih.gov/pmc/5512188","text":"2.1 Animal model\nSgo1+/LacZ mice were received from Wellcome Trust Sanger Institute (WTSI). The Sgo1+/LacZ mice have a flippase recognition target (FRT) flanked lacZ cassette inserted between exons 4 and 5 of the Sgo1 gene in a C57Bl6/N strain. A splicing acceptor site precedes the FRT for inclusion of lacZ cassette into the transcript and following polyadenylation sequence, resulting in a truncated Sgo1 mRNA transcript, and protein. The crossings were made with Sgo1+/+ and Sgo1+/LacZ and the appearance of vaginal plug was designated as Embryonic day (E) 0.5. Embryos at E9.5, E10.5, E12.5, E15.5, E18.5, and 4-month old adult mice, were collected for the experiment.","divisions":[{"label":"Title","span":{"begin":5,"end":17}}],"tracks":[]}