Id |
Subject |
Object |
Predicate |
Lexical cue |
T2699 |
0-2 |
TO |
denotes |
To |
T2700 |
3-14 |
VB |
denotes |
investigate |
T2701 |
82-91 |
VBD |
denotes |
generated |
T2702 |
15-17 |
FW |
denotes |
in |
T2703 |
18-22 |
FW |
denotes |
vivo |
T2704 |
23-26 |
DT |
denotes |
the |
T2705 |
27-36 |
NNS |
denotes |
functions |
T2706 |
37-39 |
IN |
denotes |
of |
T2707 |
40-43 |
DT |
denotes |
the |
T2708 |
63-71 |
NN |
denotes |
receptor |
T2709 |
44-62 |
NN |
denotes |
phosphatidylserine |
T2710 |
72-77 |
NN |
denotes |
Ptdsr |
T2711 |
77-79 |
, |
denotes |
, |
T2712 |
79-81 |
PRP |
denotes |
we |
T2713 |
92-93 |
DT |
denotes |
a |
T2714 |
99-105 |
NN |
denotes |
allele |
T2715 |
94-98 |
JJ |
denotes |
null |
T2716 |
106-108 |
IN |
denotes |
in |
T2717 |
109-112 |
DT |
denotes |
the |
T2718 |
113-118 |
NN |
denotes |
mouse |
T2719 |
119-121 |
IN |
denotes |
by |
T2720 |
122-126 |
NN |
denotes |
gene |
T2721 |
127-136 |
NN |
denotes |
targeting |
T2722 |
137-138 |
-LRB- |
denotes |
( |
T2723 |
151-153 |
NN |
denotes |
1c |
T2724 |
138-144 |
NN |
denotes |
Figure |
T2725 |
145-147 |
NN |
denotes |
1a |
T2726 |
147-148 |
, |
denotes |
, |
T2727 |
148-150 |
NN |
denotes |
1b |
T2728 |
150-151 |
, |
denotes |
, |
T2729 |
153-154 |
-RRB- |
denotes |
) |
T2730 |
154-155 |
. |
denotes |
. |
T2731 |
155-371 |
sentence |
denotes |
In contrast to previously described Ptdsr-knockout mice [31,32], we used Bruce4 embryonic stem (ES) cells for gene targeting [33], thus generating a Ptdsr-null allele in a pure, isogenic C57BL/6J genetic background. |
T2732 |
156-158 |
IN |
denotes |
In |
T2733 |
224-228 |
VBD |
denotes |
used |
T2734 |
159-167 |
NN |
denotes |
contrast |
T2735 |
168-170 |
IN |
denotes |
to |
T2736 |
171-181 |
RB |
denotes |
previously |
T2737 |
182-191 |
VBN |
denotes |
described |
T2738 |
207-211 |
NNS |
denotes |
mice |
T2739 |
192-197 |
NN |
denotes |
Ptdsr |
T2740 |
198-206 |
NN |
denotes |
knockout |
T2741 |
197-198 |
HYPH |
denotes |
- |
T2742 |
212-213 |
-LRB- |
denotes |
[ |
T2743 |
216-218 |
CD |
denotes |
32 |
T2744 |
213-215 |
CD |
denotes |
31 |
T2745 |
215-216 |
, |
denotes |
, |
T2746 |
218-219 |
-RRB- |
denotes |
] |
T2747 |
219-221 |
, |
denotes |
, |
T2748 |
221-223 |
PRP |
denotes |
we |
T2749 |
229-235 |
NN |
denotes |
Bruce4 |
T2750 |
256-261 |
NNS |
denotes |
cells |
T2751 |
236-245 |
JJ |
denotes |
embryonic |
T2752 |
246-250 |
NN |
denotes |
stem |
T2753 |
251-252 |
-LRB- |
denotes |
( |
T2754 |
252-254 |
NN |
denotes |
ES |
T2755 |
254-255 |
-RRB- |
denotes |
) |
T2756 |
262-265 |
IN |
denotes |
for |
T2757 |
266-270 |
NN |
denotes |
gene |
T2758 |
271-280 |
NN |
denotes |
targeting |
T2759 |
281-282 |
-LRB- |
denotes |
[ |
T2760 |
282-284 |
CD |
denotes |
33 |
T2761 |
284-285 |
-RRB- |
denotes |
] |
T2762 |
285-287 |
, |
denotes |
, |
T2763 |
287-291 |
RB |
denotes |
thus |
T2764 |
292-302 |
VBG |
denotes |
generating |
T2765 |
303-304 |
DT |
denotes |
a |
T2766 |
316-322 |
NN |
denotes |
allele |
T2767 |
305-310 |
NN |
denotes |
Ptdsr |
T2768 |
311-315 |
JJ |
denotes |
null |
T2769 |
310-311 |
HYPH |
denotes |
- |
T2770 |
323-325 |
IN |
denotes |
in |
T2771 |
326-327 |
DT |
denotes |
a |
T2772 |
360-370 |
NN |
denotes |
background |
T2773 |
328-332 |
JJ |
denotes |
pure |
T2774 |
332-334 |
, |
denotes |
, |
T2775 |
334-342 |
JJ |
denotes |
isogenic |
T2776 |
343-348 |
NN |
denotes |
C57BL |
T2777 |
349-351 |
NN |
denotes |
6J |
T2778 |
348-349 |
HYPH |
denotes |
/ |
T2779 |
352-359 |
JJ |
denotes |
genetic |
T2780 |
370-371 |
. |
denotes |
. |
T2781 |
371-473 |
sentence |
denotes |
The newly established knockout mouse line was named Ptdsrtm1Gbf (hereafter referred to as Ptdsr -/-). |
T2782 |
372-375 |
DT |
denotes |
The |
T2783 |
409-413 |
NN |
denotes |
line |
T2784 |
376-381 |
RB |
denotes |
newly |
T2785 |
382-393 |
VBN |
denotes |
established |
T2786 |
394-402 |
NN |
denotes |
knockout |
T2787 |
403-408 |
NN |
denotes |
mouse |
T2788 |
418-423 |
VBN |
denotes |
named |
T2789 |
414-417 |
VBD |
denotes |
was |
T2790 |
424-435 |
NN |
denotes |
Ptdsrtm1Gbf |
T2791 |
436-437 |
-LRB- |
denotes |
( |
T2792 |
447-455 |
VBN |
denotes |
referred |
T2793 |
437-446 |
RB |
denotes |
hereafter |
T2794 |
456-458 |
IN |
denotes |
to |
T2795 |
459-461 |
IN |
denotes |
as |
T2796 |
462-467 |
NN |
denotes |
Ptdsr |
T2797 |
468-469 |
SYM |
denotes |
- |
T2798 |
469-470 |
HYPH |
denotes |
/ |
T2799 |
470-471 |
SYM |
denotes |
- |
T2800 |
471-472 |
-RRB- |
denotes |
) |
T2801 |
472-473 |
. |
denotes |
. |
T2802 |
473-561 |
sentence |
denotes |
Heterozygous Ptdsr+/- mice were viable and fertile and showed no obvious abnormalities. |
T2803 |
474-486 |
JJ |
denotes |
Heterozygous |
T2804 |
496-500 |
NNS |
denotes |
mice |
T2805 |
487-492 |
NN |
denotes |
Ptdsr |
T2806 |
492-493 |
SYM |
denotes |
+ |
T2807 |
493-494 |
HYPH |
denotes |
/ |
T2808 |
494-495 |
SYM |
denotes |
- |
T2809 |
501-505 |
VBD |
denotes |
were |
T2810 |
506-512 |
JJ |
denotes |
viable |
T2811 |
513-516 |
CC |
denotes |
and |
T2812 |
517-524 |
JJ |
denotes |
fertile |
T2813 |
525-528 |
CC |
denotes |
and |
T2814 |
529-535 |
VBD |
denotes |
showed |
T2815 |
536-538 |
DT |
denotes |
no |
T2816 |
547-560 |
NNS |
denotes |
abnormalities |
T2817 |
539-546 |
JJ |
denotes |
obvious |
T2818 |
560-561 |
. |
denotes |
. |
T2819 |
561-639 |
sentence |
denotes |
Ptdsr +/- mice were intercrossed to generate homozygous Ptdsr-deficient mice. |
T2820 |
562-567 |
NN |
denotes |
Ptdsr |
T2821 |
572-576 |
NNS |
denotes |
mice |
T2822 |
568-569 |
SYM |
denotes |
+ |
T2823 |
569-570 |
HYPH |
denotes |
/ |
T2824 |
570-571 |
SYM |
denotes |
- |
T2825 |
582-594 |
VBN |
denotes |
intercrossed |
T2826 |
577-581 |
VBD |
denotes |
were |
T2827 |
595-597 |
TO |
denotes |
to |
T2828 |
598-606 |
VB |
denotes |
generate |
T2829 |
607-617 |
JJ |
denotes |
homozygous |
T2830 |
634-638 |
NNS |
denotes |
mice |
T2831 |
618-623 |
NN |
denotes |
Ptdsr |
T2832 |
624-633 |
JJ |
denotes |
deficient |
T2833 |
623-624 |
HYPH |
denotes |
- |
T2834 |
638-639 |
. |
denotes |
. |
T2835 |
639-795 |
sentence |
denotes |
The absence of Ptdsr expression in Ptdsr -/- embryos was confirmed by RT-PCR (data not shown), and by northern and western blotting analyses (Figure 1d,e). |
T2836 |
640-643 |
DT |
denotes |
The |
T2837 |
644-651 |
NN |
denotes |
absence |
T2838 |
697-706 |
VBN |
denotes |
confirmed |
T2839 |
652-654 |
IN |
denotes |
of |
T2840 |
655-660 |
NN |
denotes |
Ptdsr |
T2841 |
661-671 |
NN |
denotes |
expression |
T2842 |
672-674 |
IN |
denotes |
in |
T2843 |
675-680 |
NN |
denotes |
Ptdsr |
T2844 |
685-692 |
NNS |
denotes |
embryos |
T2845 |
681-682 |
SYM |
denotes |
- |
T2846 |
682-683 |
HYPH |
denotes |
/ |
T2847 |
683-684 |
SYM |
denotes |
- |
T2848 |
693-696 |
VBD |
denotes |
was |
T2849 |
707-709 |
IN |
denotes |
by |
T2850 |
710-712 |
NN |
denotes |
RT |
T2851 |
713-716 |
NN |
denotes |
PCR |
T2852 |
712-713 |
HYPH |
denotes |
- |
T2853 |
717-718 |
-LRB- |
denotes |
( |
T2854 |
727-732 |
VBN |
denotes |
shown |
T2855 |
718-722 |
NNS |
denotes |
data |
T2856 |
723-726 |
RB |
denotes |
not |
T2857 |
732-733 |
-RRB- |
denotes |
) |
T2858 |
733-735 |
, |
denotes |
, |
T2859 |
735-738 |
CC |
denotes |
and |
T2860 |
739-741 |
IN |
denotes |
by |
T2861 |
742-750 |
NNP |
denotes |
northern |
T2862 |
763-771 |
NN |
denotes |
blotting |
T2863 |
751-754 |
CC |
denotes |
and |
T2864 |
755-762 |
NNP |
denotes |
western |
T2865 |
772-780 |
NNS |
denotes |
analyses |
T2866 |
781-782 |
-LRB- |
denotes |
( |
T2867 |
792-793 |
NN |
denotes |
e |
T2868 |
782-788 |
NN |
denotes |
Figure |
T2869 |
789-791 |
NN |
denotes |
1d |
T2870 |
791-792 |
, |
denotes |
, |
T2871 |
793-794 |
-RRB- |
denotes |
) |
T2872 |
794-795 |
. |
denotes |
. |
T2873 |
795-951 |
sentence |
denotes |
Interbreeding of heterozygous mice showed that the mutation was lethal, since homozygous mutants were not detected in over 100 analyzed litters at weaning. |
T2874 |
796-809 |
NN |
denotes |
Interbreeding |
T2875 |
831-837 |
VBD |
denotes |
showed |
T2876 |
810-812 |
IN |
denotes |
of |
T2877 |
813-825 |
JJ |
denotes |
heterozygous |
T2878 |
826-830 |
NNS |
denotes |
mice |
T2879 |
838-842 |
IN |
denotes |
that |
T2880 |
856-859 |
VBD |
denotes |
was |
T2881 |
843-846 |
DT |
denotes |
the |
T2882 |
847-855 |
NN |
denotes |
mutation |
T2883 |
860-866 |
JJ |
denotes |
lethal |
T2884 |
866-868 |
, |
denotes |
, |
T2885 |
868-873 |
IN |
denotes |
since |
T2886 |
902-910 |
VBN |
denotes |
detected |
T2887 |
874-884 |
JJ |
denotes |
homozygous |
T2888 |
885-892 |
NNS |
denotes |
mutants |
T2889 |
893-897 |
VBD |
denotes |
were |
T2890 |
898-901 |
RB |
denotes |
not |
T2891 |
911-913 |
IN |
denotes |
in |
T2892 |
914-918 |
IN |
denotes |
over |
T2893 |
919-922 |
CD |
denotes |
100 |
T2894 |
932-939 |
NNS |
denotes |
litters |
T2895 |
923-931 |
VBN |
denotes |
analyzed |
T2896 |
940-942 |
IN |
denotes |
at |
T2897 |
943-950 |
NN |
denotes |
weaning |
T2898 |
950-951 |
. |
denotes |
. |
T2899 |
951-1110 |
sentence |
denotes |
To determine the stages of embryonic development affected by the Ptdsrtm1Gbf mutation, timed breedings were followed by PCR genotyping (Figure 1c) of embryos. |
T2900 |
952-954 |
TO |
denotes |
To |
T2901 |
955-964 |
VB |
denotes |
determine |
T2902 |
1060-1068 |
VBN |
denotes |
followed |
T2903 |
965-968 |
DT |
denotes |
the |
T2904 |
969-975 |
NNS |
denotes |
stages |
T2905 |
976-978 |
IN |
denotes |
of |
T2906 |
979-988 |
JJ |
denotes |
embryonic |
T2907 |
989-1000 |
NN |
denotes |
development |
T2908 |
1001-1009 |
VBN |
denotes |
affected |
T2909 |
1010-1012 |
IN |
denotes |
by |
T2910 |
1013-1016 |
DT |
denotes |
the |
T2911 |
1029-1037 |
NN |
denotes |
mutation |
T2912 |
1017-1028 |
NN |
denotes |
Ptdsrtm1Gbf |
T2913 |
1037-1039 |
, |
denotes |
, |
T2914 |
1039-1044 |
VBN |
denotes |
timed |
T2915 |
1045-1054 |
NNS |
denotes |
breedings |
T2916 |
1055-1059 |
VBD |
denotes |
were |
T2917 |
1069-1071 |
IN |
denotes |
by |
T2918 |
1072-1075 |
NN |
denotes |
PCR |
T2919 |
1076-1086 |
NN |
denotes |
genotyping |
T2920 |
1087-1088 |
-LRB- |
denotes |
( |
T2921 |
1095-1097 |
NN |
denotes |
1c |
T2922 |
1088-1094 |
NN |
denotes |
Figure |
T2923 |
1097-1098 |
-RRB- |
denotes |
) |
T2924 |
1099-1101 |
IN |
denotes |
of |
T2925 |
1102-1109 |
NNS |
denotes |
embryos |
T2926 |
1109-1110 |
. |
denotes |
. |
T2927 |
1110-1211 |
sentence |
denotes |
We recovered fewer than the expected number of homozygous embryos from intercrosses of Ptdsr+/-mice. |
T2928 |
1111-1113 |
PRP |
denotes |
We |
T2929 |
1114-1123 |
VBD |
denotes |
recovered |
T2930 |
1124-1129 |
JJR |
denotes |
fewer |
T2931 |
1130-1134 |
IN |
denotes |
than |
T2932 |
1135-1138 |
DT |
denotes |
the |
T2933 |
1148-1154 |
NN |
denotes |
number |
T2934 |
1139-1147 |
VBN |
denotes |
expected |
T2935 |
1155-1157 |
IN |
denotes |
of |
T2936 |
1158-1168 |
JJ |
denotes |
homozygous |
T2937 |
1169-1176 |
NNS |
denotes |
embryos |
T2938 |
1177-1181 |
IN |
denotes |
from |
T2939 |
1182-1194 |
NNS |
denotes |
intercrosses |
T2940 |
1195-1197 |
IN |
denotes |
of |
T2941 |
1198-1203 |
NN |
denotes |
Ptdsr |
T2942 |
1206-1210 |
NNS |
denotes |
mice |
T2943 |
1203-1204 |
SYM |
denotes |
+ |
T2944 |
1204-1205 |
HYPH |
denotes |
/ |
T2945 |
1205-1206 |
SYM |
denotes |
- |
T2946 |
1210-1211 |
. |
denotes |
. |
T2947 |
1211-1459 |
sentence |
denotes |
From a total of 1,031 embryos analyzed between gestational day (E) 9.5 and E18.5, 198 (19.2%) Ptdsr-deficient homozygous embryos were harvested, indicating that the introduced mutation is associated with a low rate of embryonic lethality in utero. |
T2948 |
1212-1216 |
IN |
denotes |
From |
T2949 |
1346-1355 |
VBN |
denotes |
harvested |
T2950 |
1217-1218 |
DT |
denotes |
a |
T2951 |
1219-1224 |
NN |
denotes |
total |
T2952 |
1225-1227 |
IN |
denotes |
of |
T2953 |
1228-1233 |
CD |
denotes |
1,031 |
T2954 |
1234-1241 |
NNS |
denotes |
embryos |
T2955 |
1242-1250 |
VBN |
denotes |
analyzed |
T2956 |
1251-1258 |
IN |
denotes |
between |
T2957 |
1259-1270 |
JJ |
denotes |
gestational |
T2958 |
1276-1277 |
NN |
denotes |
E |
T2959 |
1271-1274 |
NN |
denotes |
day |
T2960 |
1275-1276 |
-LRB- |
denotes |
( |
T2961 |
1277-1278 |
-RRB- |
denotes |
) |
T2962 |
1279-1282 |
CD |
denotes |
9.5 |
T2963 |
1283-1286 |
CC |
denotes |
and |
T2964 |
1287-1292 |
NN |
denotes |
E18.5 |
T2965 |
1292-1294 |
, |
denotes |
, |
T2966 |
1294-1297 |
CD |
denotes |
198 |
T2967 |
1333-1340 |
NNS |
denotes |
embryos |
T2968 |
1298-1299 |
-LRB- |
denotes |
( |
T2969 |
1303-1304 |
NN |
denotes |
% |
T2970 |
1299-1303 |
CD |
denotes |
19.2 |
T2971 |
1304-1305 |
-RRB- |
denotes |
) |
T2972 |
1306-1311 |
NN |
denotes |
Ptdsr |
T2973 |
1312-1321 |
JJ |
denotes |
deficient |
T2974 |
1311-1312 |
HYPH |
denotes |
- |
T2975 |
1322-1332 |
JJ |
denotes |
homozygous |
T2976 |
1341-1345 |
VBD |
denotes |
were |
T2977 |
1355-1357 |
, |
denotes |
, |
T2978 |
1357-1367 |
VBG |
denotes |
indicating |
T2979 |
1368-1372 |
IN |
denotes |
that |
T2980 |
1400-1410 |
VBN |
denotes |
associated |
T2981 |
1373-1376 |
DT |
denotes |
the |
T2982 |
1388-1396 |
NN |
denotes |
mutation |
T2983 |
1377-1387 |
VBN |
denotes |
introduced |
T2984 |
1397-1399 |
VBZ |
denotes |
is |
T2985 |
1411-1415 |
IN |
denotes |
with |
T2986 |
1416-1417 |
DT |
denotes |
a |
T2987 |
1422-1426 |
NN |
denotes |
rate |
T2988 |
1418-1421 |
JJ |
denotes |
low |
T2989 |
1427-1429 |
IN |
denotes |
of |
T2990 |
1430-1439 |
JJ |
denotes |
embryonic |
T2991 |
1440-1449 |
NN |
denotes |
lethality |
T2992 |
1450-1452 |
FW |
denotes |
in |
T2993 |
1453-1458 |
FW |
denotes |
utero |
T2994 |
1458-1459 |
. |
denotes |
. |
R1349 |
T2699 |
T2700 |
aux |
To,investigate |
R1350 |
T2700 |
T2701 |
advcl |
investigate,generated |
R1351 |
T2702 |
T2703 |
advmod |
in,vivo |
R1352 |
T2703 |
T2700 |
advmod |
vivo,investigate |
R1353 |
T2704 |
T2705 |
det |
the,functions |
R1354 |
T2705 |
T2700 |
dobj |
functions,investigate |
R1355 |
T2706 |
T2705 |
prep |
of,functions |
R1356 |
T2707 |
T2708 |
det |
the,receptor |
R1357 |
T2708 |
T2706 |
pobj |
receptor,of |
R1358 |
T2709 |
T2708 |
compound |
phosphatidylserine,receptor |
R1359 |
T2710 |
T2708 |
appos |
Ptdsr,receptor |
R1360 |
T2711 |
T2701 |
punct |
", ",generated |
R1361 |
T2712 |
T2701 |
nsubj |
we,generated |
R1362 |
T2713 |
T2714 |
det |
a,allele |
R1363 |
T2714 |
T2701 |
dobj |
allele,generated |
R1364 |
T2715 |
T2714 |
amod |
null,allele |
R1365 |
T2716 |
T2701 |
prep |
in,generated |
R1366 |
T2717 |
T2718 |
det |
the,mouse |
R1367 |
T2718 |
T2716 |
pobj |
mouse,in |
R1368 |
T2719 |
T2701 |
prep |
by,generated |
R1369 |
T2720 |
T2721 |
compound |
gene,targeting |
R1370 |
T2721 |
T2719 |
pobj |
targeting,by |
R1371 |
T2722 |
T2723 |
punct |
(,1c |
R1372 |
T2723 |
T2701 |
parataxis |
1c,generated |
R1373 |
T2724 |
T2723 |
nmod |
Figure,1c |
R1374 |
T2725 |
T2723 |
nmod |
1a,1c |
R1375 |
T2726 |
T2723 |
punct |
",",1c |
R1376 |
T2727 |
T2723 |
nmod |
1b,1c |
R1377 |
T2728 |
T2723 |
punct |
",",1c |
R1378 |
T2729 |
T2723 |
punct |
),1c |
R1379 |
T2730 |
T2701 |
punct |
.,generated |
R1380 |
T2732 |
T2733 |
prep |
In,used |
R1381 |
T2734 |
T2732 |
pobj |
contrast,In |
R1382 |
T2735 |
T2734 |
prep |
to,contrast |
R1383 |
T2736 |
T2737 |
advmod |
previously,described |
R1384 |
T2737 |
T2738 |
amod |
described,mice |
R1385 |
T2738 |
T2735 |
pobj |
mice,to |
R1386 |
T2739 |
T2740 |
compound |
Ptdsr,knockout |
R1387 |
T2740 |
T2738 |
compound |
knockout,mice |
R1388 |
T2741 |
T2740 |
punct |
-,knockout |
R1389 |
T2742 |
T2743 |
punct |
[,32 |
R1390 |
T2743 |
T2732 |
parataxis |
32,In |
R1391 |
T2744 |
T2743 |
nummod |
31,32 |
R1392 |
T2745 |
T2743 |
punct |
",",32 |
R1393 |
T2746 |
T2743 |
punct |
],32 |
R1394 |
T2747 |
T2733 |
punct |
", ",used |
R1395 |
T2748 |
T2733 |
nsubj |
we,used |
R1396 |
T2749 |
T2750 |
nmod |
Bruce4,cells |
R1397 |
T2750 |
T2733 |
dobj |
cells,used |
R1398 |
T2751 |
T2752 |
amod |
embryonic,stem |
R1399 |
T2752 |
T2750 |
nmod |
stem,cells |
R1400 |
T2753 |
T2752 |
punct |
(,stem |
R1401 |
T2754 |
T2752 |
appos |
ES,stem |
R1402 |
T2755 |
T2752 |
punct |
),stem |
R1403 |
T2756 |
T2733 |
prep |
for,used |
R1404 |
T2757 |
T2758 |
compound |
gene,targeting |
R1405 |
T2758 |
T2756 |
pobj |
targeting,for |
R1406 |
T2759 |
T2760 |
punct |
[,33 |
R1407 |
T2760 |
T2733 |
parataxis |
33,used |
R1408 |
T2761 |
T2760 |
punct |
],33 |
R1409 |
T2762 |
T2733 |
punct |
", ",used |
R1410 |
T2763 |
T2764 |
advmod |
thus,generating |
R1411 |
T2764 |
T2733 |
advcl |
generating,used |
R1412 |
T2765 |
T2766 |
det |
a,allele |
R1413 |
T2766 |
T2764 |
dobj |
allele,generating |
R1414 |
T2767 |
T2768 |
npadvmod |
Ptdsr,null |
R1415 |
T2768 |
T2766 |
amod |
null,allele |
R1416 |
T2769 |
T2768 |
punct |
-,null |
R1417 |
T2770 |
T2764 |
prep |
in,generating |
R1418 |
T2771 |
T2772 |
det |
a,background |
R1419 |
T2772 |
T2770 |
pobj |
background,in |
R1420 |
T2773 |
T2772 |
amod |
pure,background |
R1421 |
T2774 |
T2772 |
punct |
", ",background |
R1422 |
T2775 |
T2772 |
amod |
isogenic,background |
R1423 |
T2776 |
T2777 |
nmod |
C57BL,6J |
R1424 |
T2777 |
T2772 |
nmod |
6J,background |
R1425 |
T2778 |
T2777 |
punct |
/,6J |
R1426 |
T2779 |
T2772 |
amod |
genetic,background |
R1427 |
T2780 |
T2733 |
punct |
.,used |
R1428 |
T2782 |
T2783 |
det |
The,line |
R1429 |
T2783 |
T2788 |
nsubjpass |
line,named |
R1430 |
T2784 |
T2785 |
advmod |
newly,established |
R1431 |
T2785 |
T2783 |
amod |
established,line |
R1432 |
T2786 |
T2783 |
compound |
knockout,line |
R1433 |
T2787 |
T2783 |
compound |
mouse,line |
R1434 |
T2789 |
T2788 |
auxpass |
was,named |
R1435 |
T2790 |
T2788 |
oprd |
Ptdsrtm1Gbf,named |
R1436 |
T2791 |
T2792 |
punct |
(,referred |
R1437 |
T2792 |
T2790 |
parataxis |
referred,Ptdsrtm1Gbf |
R1438 |
T2793 |
T2792 |
advmod |
hereafter,referred |
R1439 |
T2794 |
T2792 |
prep |
to,referred |
R1440 |
T2795 |
T2792 |
prep |
as,referred |
R1441 |
T2796 |
T2795 |
pobj |
Ptdsr,as |
R1442 |
T2797 |
T2796 |
punct |
-,Ptdsr |
R1443 |
T2798 |
T2796 |
punct |
/,Ptdsr |
R1444 |
T2799 |
T2796 |
punct |
-,Ptdsr |
R1445 |
T2800 |
T2792 |
punct |
),referred |
R1446 |
T2801 |
T2788 |
punct |
.,named |
R1447 |
T2803 |
T2804 |
amod |
Heterozygous,mice |
R1448 |
T2804 |
T2809 |
nsubj |
mice,were |
R1449 |
T2805 |
T2804 |
nmod |
Ptdsr,mice |
R1450 |
T2806 |
T2805 |
punct |
+,Ptdsr |
R1451 |
T2807 |
T2805 |
punct |
/,Ptdsr |
R1452 |
T2808 |
T2805 |
punct |
-,Ptdsr |
R1453 |
T2810 |
T2809 |
acomp |
viable,were |
R1454 |
T2811 |
T2810 |
cc |
and,viable |
R1455 |
T2812 |
T2810 |
conj |
fertile,viable |
R1456 |
T2813 |
T2809 |
cc |
and,were |
R1457 |
T2814 |
T2809 |
conj |
showed,were |
R1458 |
T2815 |
T2816 |
det |
no,abnormalities |
R1459 |
T2816 |
T2814 |
dobj |
abnormalities,showed |
R1460 |
T2817 |
T2816 |
amod |
obvious,abnormalities |
R1461 |
T2818 |
T2809 |
punct |
.,were |
R1462 |
T2820 |
T2821 |
nmod |
Ptdsr,mice |
R1463 |
T2821 |
T2825 |
nsubjpass |
mice,intercrossed |
R1464 |
T2822 |
T2820 |
punct |
+,Ptdsr |
R1465 |
T2823 |
T2820 |
punct |
/,Ptdsr |
R1466 |
T2824 |
T2820 |
punct |
-,Ptdsr |
R1467 |
T2826 |
T2825 |
auxpass |
were,intercrossed |
R1468 |
T2827 |
T2828 |
aux |
to,generate |
R1469 |
T2828 |
T2825 |
advcl |
generate,intercrossed |
R1470 |
T2829 |
T2830 |
amod |
homozygous,mice |
R1471 |
T2830 |
T2828 |
dobj |
mice,generate |
R1472 |
T2831 |
T2832 |
npadvmod |
Ptdsr,deficient |
R1473 |
T2832 |
T2830 |
amod |
deficient,mice |
R1474 |
T2833 |
T2832 |
punct |
-,deficient |
R1475 |
T2834 |
T2825 |
punct |
.,intercrossed |
R1476 |
T2836 |
T2837 |
det |
The,absence |
R1477 |
T2837 |
T2838 |
nsubjpass |
absence,confirmed |
R1478 |
T2839 |
T2837 |
prep |
of,absence |
R1479 |
T2840 |
T2841 |
compound |
Ptdsr,expression |
R1480 |
T2841 |
T2839 |
pobj |
expression,of |
R1481 |
T2842 |
T2837 |
prep |
in,absence |
R1482 |
T2843 |
T2844 |
nmod |
Ptdsr,embryos |
R1483 |
T2844 |
T2842 |
pobj |
embryos,in |
R1484 |
T2845 |
T2843 |
punct |
-,Ptdsr |
R1485 |
T2846 |
T2843 |
punct |
/,Ptdsr |
R1486 |
T2847 |
T2843 |
punct |
-,Ptdsr |
R1487 |
T2848 |
T2838 |
auxpass |
was,confirmed |
R1488 |
T2849 |
T2838 |
prep |
by,confirmed |
R1489 |
T2850 |
T2851 |
compound |
RT,PCR |
R1490 |
T2851 |
T2849 |
pobj |
PCR,by |
R1491 |
T2852 |
T2851 |
punct |
-,PCR |
R1492 |
T2853 |
T2854 |
punct |
(,shown |
R1493 |
T2854 |
T2849 |
parataxis |
shown,by |
R1494 |
T2855 |
T2854 |
nsubj |
data,shown |
R1495 |
T2856 |
T2854 |
neg |
not,shown |
R1496 |
T2857 |
T2854 |
punct |
),shown |
R1497 |
T2858 |
T2849 |
punct |
", ",by |
R1498 |
T2859 |
T2849 |
cc |
and,by |
R1499 |
T2860 |
T2849 |
conj |
by,by |
R1500 |
T2861 |
T2862 |
nmod |
northern,blotting |
R1501 |
T2862 |
T2865 |
compound |
blotting,analyses |
R1502 |
T2863 |
T2861 |
cc |
and,northern |
R1503 |
T2864 |
T2861 |
conj |
western,northern |
R1504 |
T2865 |
T2860 |
pobj |
analyses,by |
R1505 |
T2866 |
T2867 |
punct |
(,e |
R1506 |
T2867 |
T2838 |
parataxis |
e,confirmed |
R1507 |
T2868 |
T2867 |
nmod |
Figure,e |
R1508 |
T2869 |
T2867 |
nmod |
1d,e |
R1509 |
T2870 |
T2867 |
punct |
",",e |
R1510 |
T2871 |
T2867 |
punct |
),e |
R1511 |
T2872 |
T2838 |
punct |
.,confirmed |
R1512 |
T2874 |
T2875 |
nsubj |
Interbreeding,showed |
R1513 |
T2876 |
T2874 |
prep |
of,Interbreeding |
R1514 |
T2877 |
T2878 |
amod |
heterozygous,mice |
R1515 |
T2878 |
T2876 |
pobj |
mice,of |
R1516 |
T2879 |
T2880 |
mark |
that,was |
R1517 |
T2880 |
T2875 |
ccomp |
was,showed |
R1518 |
T2881 |
T2882 |
det |
the,mutation |
R1519 |
T2882 |
T2880 |
nsubj |
mutation,was |
R1520 |
T2883 |
T2880 |
acomp |
lethal,was |
R1521 |
T2884 |
T2880 |
punct |
", ",was |
R1522 |
T2885 |
T2886 |
mark |
since,detected |
R1523 |
T2886 |
T2880 |
advcl |
detected,was |
R1524 |
T2887 |
T2888 |
amod |
homozygous,mutants |
R1525 |
T2888 |
T2886 |
nsubjpass |
mutants,detected |
R1526 |
T2889 |
T2886 |
auxpass |
were,detected |
R1527 |
T2890 |
T2886 |
neg |
not,detected |
R1528 |
T2891 |
T2886 |
prep |
in,detected |
R1529 |
T2892 |
T2893 |
quantmod |
over,100 |
R1530 |
T2893 |
T2894 |
nummod |
100,litters |
R1531 |
T2894 |
T2891 |
pobj |
litters,in |
R1532 |
T2895 |
T2894 |
amod |
analyzed,litters |
R1533 |
T2896 |
T2886 |
prep |
at,detected |
R1534 |
T2897 |
T2896 |
pobj |
weaning,at |
R1535 |
T2898 |
T2875 |
punct |
.,showed |
R1536 |
T2900 |
T2901 |
aux |
To,determine |
R1537 |
T2901 |
T2902 |
advcl |
determine,followed |
R1538 |
T2903 |
T2904 |
det |
the,stages |
R1539 |
T2904 |
T2901 |
dobj |
stages,determine |
R1540 |
T2905 |
T2904 |
prep |
of,stages |
R1541 |
T2906 |
T2907 |
amod |
embryonic,development |
R1542 |
T2907 |
T2905 |
pobj |
development,of |
R1543 |
T2908 |
T2907 |
acl |
affected,development |
R1544 |
T2909 |
T2908 |
agent |
by,affected |
R1545 |
T2910 |
T2911 |
det |
the,mutation |
R1546 |
T2911 |
T2909 |
pobj |
mutation,by |
R1547 |
T2912 |
T2911 |
compound |
Ptdsrtm1Gbf,mutation |
R1548 |
T2913 |
T2902 |
punct |
", ",followed |
R1549 |
T2914 |
T2915 |
amod |
timed,breedings |
R1550 |
T2915 |
T2902 |
nsubjpass |
breedings,followed |
R1551 |
T2916 |
T2902 |
auxpass |
were,followed |
R1552 |
T2917 |
T2902 |
prep |
by,followed |
R1553 |
T2918 |
T2919 |
compound |
PCR,genotyping |
R1554 |
T2919 |
T2917 |
pobj |
genotyping,by |
R1555 |
T2920 |
T2921 |
punct |
(,1c |
R1556 |
T2921 |
T2919 |
parataxis |
1c,genotyping |
R1557 |
T2922 |
T2921 |
compound |
Figure,1c |
R1558 |
T2923 |
T2921 |
punct |
),1c |
R1559 |
T2924 |
T2919 |
prep |
of,genotyping |
R1560 |
T2925 |
T2924 |
pobj |
embryos,of |
R1561 |
T2926 |
T2902 |
punct |
.,followed |
R1562 |
T2928 |
T2929 |
nsubj |
We,recovered |
R1563 |
T2930 |
T2929 |
dobj |
fewer,recovered |
R1564 |
T2931 |
T2930 |
prep |
than,fewer |
R1565 |
T2932 |
T2933 |
det |
the,number |
R1566 |
T2933 |
T2931 |
pobj |
number,than |
R1567 |
T2934 |
T2933 |
amod |
expected,number |
R1568 |
T2935 |
T2933 |
prep |
of,number |
R1569 |
T2936 |
T2937 |
amod |
homozygous,embryos |
R1570 |
T2937 |
T2935 |
pobj |
embryos,of |
R1571 |
T2938 |
T2929 |
prep |
from,recovered |
R1572 |
T2939 |
T2938 |
pobj |
intercrosses,from |
R1573 |
T2940 |
T2939 |
prep |
of,intercrosses |
R1574 |
T2941 |
T2942 |
nmod |
Ptdsr,mice |
R1575 |
T2942 |
T2940 |
pobj |
mice,of |
R1576 |
T2943 |
T2941 |
punct |
+,Ptdsr |
R1577 |
T2944 |
T2941 |
punct |
/,Ptdsr |
R1578 |
T2945 |
T2941 |
punct |
-,Ptdsr |
R1579 |
T2946 |
T2929 |
punct |
.,recovered |
R1580 |
T2948 |
T2949 |
prep |
From,harvested |
R1581 |
T2950 |
T2951 |
det |
a,total |
R1582 |
T2951 |
T2948 |
pobj |
total,From |
R1583 |
T2952 |
T2951 |
prep |
of,total |
R1584 |
T2953 |
T2954 |
nummod |
"1,031",embryos |
R1585 |
T2954 |
T2952 |
pobj |
embryos,of |
R1586 |
T2955 |
T2954 |
acl |
analyzed,embryos |
R1587 |
T2956 |
T2955 |
prep |
between,analyzed |
R1588 |
T2957 |
T2958 |
amod |
gestational,E |
R1589 |
T2958 |
T2956 |
pobj |
E,between |
R1590 |
T2959 |
T2958 |
nmod |
day,E |
R1591 |
T2960 |
T2958 |
punct |
(,E |
R1592 |
T2961 |
T2958 |
punct |
),E |
R1593 |
T2962 |
T2958 |
nummod |
9.5,E |
R1594 |
T2963 |
T2958 |
cc |
and,E |
R1595 |
T2964 |
T2958 |
conj |
E18.5,E |
R1596 |
T2965 |
T2949 |
punct |
", ",harvested |
R1597 |
T2966 |
T2967 |
nummod |
198,embryos |
R1598 |
T2967 |
T2949 |
nsubjpass |
embryos,harvested |
R1599 |
T2968 |
T2969 |
punct |
(,% |
R1600 |
T2969 |
T2966 |
parataxis |
%,198 |
R1601 |
T2970 |
T2969 |
nummod |
19.2,% |
R1602 |
T2971 |
T2969 |
punct |
),% |
R1603 |
T2972 |
T2973 |
npadvmod |
Ptdsr,deficient |
R1604 |
T2973 |
T2967 |
amod |
deficient,embryos |
R1605 |
T2974 |
T2973 |
punct |
-,deficient |
R1606 |
T2975 |
T2967 |
amod |
homozygous,embryos |
R1607 |
T2976 |
T2949 |
auxpass |
were,harvested |
R1608 |
T2977 |
T2949 |
punct |
", ",harvested |
R1609 |
T2978 |
T2949 |
advcl |
indicating,harvested |
R1610 |
T2979 |
T2980 |
mark |
that,associated |
R1611 |
T2980 |
T2978 |
ccomp |
associated,indicating |
R1612 |
T2981 |
T2982 |
det |
the,mutation |
R1613 |
T2982 |
T2980 |
nsubjpass |
mutation,associated |
R1614 |
T2983 |
T2982 |
amod |
introduced,mutation |
R1615 |
T2984 |
T2980 |
auxpass |
is,associated |
R1616 |
T2985 |
T2980 |
prep |
with,associated |
R1617 |
T2986 |
T2987 |
det |
a,rate |
R1618 |
T2987 |
T2985 |
pobj |
rate,with |
R1619 |
T2988 |
T2987 |
amod |
low,rate |
R1620 |
T2989 |
T2987 |
prep |
of,rate |
R1621 |
T2990 |
T2991 |
amod |
embryonic,lethality |
R1622 |
T2991 |
T2989 |
pobj |
lethality,of |
R1623 |
T2992 |
T2993 |
advmod |
in,utero |
R1624 |
T2993 |
T2991 |
advmod |
utero,lethality |
R1625 |
T2994 |
T2949 |
punct |
.,harvested |