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PMC:549712 / 56825-57713 JSONTXT

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Id Subject Object Predicate Lexical cue
T16464 0-11 NN denotes Preparation
T16465 12-14 IN denotes of
T16466 15-20 JJ denotes fetal
T16467 21-26 NN denotes liver
T16468 27-34 VBN denotes derived
T16469 26-27 HYPH denotes -
T16470 35-46 NNS denotes macrophages
T16471 47-48 -LRB- denotes (
T16472 48-53 NNS denotes FLDMs
T16473 53-54 -RRB- denotes )
T16474 54-191 sentence denotes Fetal livers were excised from embryos at E12.5 and E13.5, respectively, washed in PBS and dissociated enzymatically for 60 min at 37°C.
T16475 55-60 JJ denotes Fetal
T16476 61-67 NNS denotes livers
T16477 73-80 VBN denotes excised
T16478 68-72 VBD denotes were
T16479 81-85 IN denotes from
T16480 86-93 NNS denotes embryos
T16481 94-96 IN denotes at
T16482 97-102 NN denotes E12.5
T16483 103-106 CC denotes and
T16484 107-112 NN denotes E13.5
T16485 112-114 , denotes ,
T16486 114-126 RB denotes respectively
T16487 126-128 , denotes ,
T16488 128-134 VBN denotes washed
T16489 135-137 IN denotes in
T16490 138-141 NN denotes PBS
T16491 142-145 CC denotes and
T16492 146-157 VBN denotes dissociated
T16493 158-171 RB denotes enzymatically
T16494 172-175 IN denotes for
T16495 176-178 CD denotes 60
T16496 179-182 NNS denotes min
T16497 183-185 IN denotes at
T16498 186-188 CD denotes 37
T16499 188-190 NN denotes °C
T16500 190-191 . denotes .
T16501 191-337 sentence denotes The digestion buffer (150 μl per liver) comprised 0.6 U/ml dispase I (Roche), 0.1% collagenase D (Roche), 10 U DNase (Roche), and 20% FCS in PBS.
T16502 192-195 DT denotes The
T16503 206-212 NN denotes buffer
T16504 196-205 NN denotes digestion
T16505 232-241 VBD denotes comprised
T16506 213-214 -LRB- denotes (
T16507 218-220 NNS denotes μl
T16508 214-217 CD denotes 150
T16509 221-224 IN denotes per
T16510 225-230 NN denotes liver
T16511 230-231 -RRB- denotes )
T16512 242-245 CD denotes 0.6
T16513 246-247 NN denotes U
T16514 251-258 NN denotes dispase
T16515 247-248 SYM denotes /
T16516 248-250 NN denotes ml
T16517 259-260 CD denotes I
T16518 261-262 -LRB- denotes (
T16519 262-267 NNP denotes Roche
T16520 267-268 -RRB- denotes )
T16521 268-270 , denotes ,
T16522 270-273 CD denotes 0.1
T16523 273-274 NN denotes %
T16524 287-288 NN denotes D
T16525 275-286 NN denotes collagenase
T16526 289-290 -LRB- denotes (
T16527 290-295 NNP denotes Roche
T16528 295-296 -RRB- denotes )
T16529 296-298 , denotes ,
T16530 298-300 CD denotes 10
T16531 301-302 NN denotes U
T16532 303-308 NN denotes DNase
T16533 309-310 -LRB- denotes (
T16534 310-315 NNP denotes Roche
T16535 315-316 -RRB- denotes )
T16536 316-318 , denotes ,
T16537 318-321 CC denotes and
T16538 322-324 CD denotes 20
T16539 324-325 NN denotes %
T16540 326-329 NN denotes FCS
T16541 330-332 IN denotes in
T16542 333-336 NN denotes PBS
T16543 336-337 . denotes .
T16544 337-689 sentence denotes X-Vivo 15 medium (Cambrex, East Rutherford, USA) was added to the resulting cell suspension, and after centrifugation (200 × g; 3 min) cells were resuspended in X-Vivo 15 medium supplemented with 50 ng/ml macrophage colony-stimulating factor (M-CSF; Sigma-Aldrich, St. Louis, USA) and cultured on non-treated tissue-culture dishes at 37°C with 5% CO2.
T16545 338-339 NN denotes X
T16546 348-354 NN denotes medium
T16547 339-340 HYPH denotes -
T16548 340-344 NN denotes Vivo
T16549 345-347 CD denotes 15
T16550 391-396 VBN denotes added
T16551 355-356 -LRB- denotes (
T16552 356-363 NNP denotes Cambrex
T16553 363-365 , denotes ,
T16554 365-369 NNP denotes East
T16555 370-380 NNP denotes Rutherford
T16556 380-382 , denotes ,
T16557 382-385 NNP denotes USA
T16558 385-386 -RRB- denotes )
T16559 387-390 VBD denotes was
T16560 397-399 IN denotes to
T16561 400-403 DT denotes the
T16562 419-429 NN denotes suspension
T16563 404-413 VBG denotes resulting
T16564 414-418 NN denotes cell
T16565 429-431 , denotes ,
T16566 431-434 CC denotes and
T16567 435-440 IN denotes after
T16568 484-495 VBN denotes resuspended
T16569 441-455 NN denotes centrifugation
T16570 456-457 -LRB- denotes (
T16571 463-464 NN denotes g
T16572 457-460 CD denotes 200
T16573 461-462 SYM denotes ×
T16574 464-465 : denotes ;
T16575 466-467 CD denotes 3
T16576 468-471 NNS denotes min
T16577 471-472 -RRB- denotes )
T16578 473-478 NNS denotes cells
T16579 479-483 VBD denotes were
T16580 496-498 IN denotes in
T16581 499-500 NN denotes X
T16582 501-505 NN denotes Vivo
T16583 500-501 HYPH denotes -
T16584 509-515 NN denotes medium
T16585 506-508 CD denotes 15
T16586 516-528 VBN denotes supplemented
T16587 529-533 IN denotes with
T16588 534-536 CD denotes 50
T16589 537-539 NN denotes ng
T16590 573-579 NN denotes factor
T16591 539-540 SYM denotes /
T16592 540-542 NN denotes ml
T16593 543-553 NN denotes macrophage
T16594 554-560 NN denotes colony
T16595 561-572 VBG denotes stimulating
T16596 560-561 HYPH denotes -
T16597 580-581 -LRB- denotes (
T16598 594-601 NNP denotes Aldrich
T16599 581-582 NN denotes M
T16600 583-586 NN denotes CSF
T16601 582-583 HYPH denotes -
T16602 586-587 : denotes ;
T16603 588-593 NNP denotes Sigma
T16604 593-594 HYPH denotes -
T16605 601-603 , denotes ,
T16606 603-606 NNP denotes St.
T16607 607-612 NNP denotes Louis
T16608 612-614 , denotes ,
T16609 614-617 NNP denotes USA
T16610 617-618 -RRB- denotes )
T16611 619-622 CC denotes and
T16612 623-631 VBN denotes cultured
T16613 632-634 IN denotes on
T16614 635-646 JJ denotes non-treated
T16615 654-661 NN denotes culture
T16616 647-653 NN denotes tissue
T16617 653-654 HYPH denotes -
T16618 662-668 NNS denotes dishes
T16619 669-671 IN denotes at
T16620 672-674 CD denotes 37
T16621 674-676 NN denotes °C
T16622 677-681 IN denotes with
T16623 682-683 CD denotes 5
T16624 683-684 NN denotes %
T16625 685-688 NN denotes CO2
T16626 688-689 . denotes .
T16627 689-757 sentence denotes Every second or third day the medium was changed by centrifugation.
T16628 690-695 DT denotes Every
T16629 712-715 NN denotes day
T16630 696-702 JJ denotes second
T16631 703-705 CC denotes or
T16632 706-711 JJ denotes third
T16633 731-738 VBN denotes changed
T16634 716-719 DT denotes the
T16635 720-726 NN denotes medium
T16636 727-730 VBD denotes was
T16637 739-741 IN denotes by
T16638 742-756 NN denotes centrifugation
T16639 756-757 . denotes .
T16640 757-888 sentence denotes Following withdrawal of M-CSF on day 6 after excision, adherent cells were cultured for an additional 24-48 h in X-Vivo 15 medium.
T16641 758-767 IN denotes Following
T16642 833-841 VBN denotes cultured
T16643 768-778 NN denotes withdrawal
T16644 779-781 IN denotes of
T16645 782-783 NN denotes M
T16646 784-787 NN denotes CSF
T16647 783-784 HYPH denotes -
T16648 788-790 IN denotes on
T16649 791-794 NN denotes day
T16650 795-796 CD denotes 6
T16651 797-802 IN denotes after
T16652 803-811 NN denotes excision
T16653 811-813 , denotes ,
T16654 813-821 JJ denotes adherent
T16655 822-827 NNS denotes cells
T16656 828-832 VBD denotes were
T16657 842-845 IN denotes for
T16658 846-848 DT denotes an
T16659 866-867 NNS denotes h
T16660 849-859 JJ denotes additional
T16661 860-862 CD denotes 24
T16662 863-865 CD denotes 48
T16663 862-863 HYPH denotes -
T16664 868-870 IN denotes in
T16665 871-872 NN denotes X
T16666 873-877 NN denotes Vivo
T16667 872-873 HYPH denotes -
T16668 881-887 NN denotes medium
T16669 878-880 CD denotes 15
T16670 887-888 . denotes .
R10078 T16465 T16464 prep of,Preparation
R10079 T16466 T16467 amod fetal,liver
R10080 T16467 T16468 npadvmod liver,derived
R10081 T16468 T16470 amod derived,macrophages
R10082 T16469 T16468 punct -,derived
R10083 T16470 T16465 pobj macrophages,of
R10084 T16471 T16470 punct (,macrophages
R10085 T16472 T16470 appos FLDMs,macrophages
R10086 T16473 T16470 punct ),macrophages
R10087 T16475 T16476 amod Fetal,livers
R10088 T16476 T16477 nsubjpass livers,excised
R10089 T16478 T16477 auxpass were,excised
R10090 T16479 T16477 prep from,excised
R10091 T16480 T16479 pobj embryos,from
R10092 T16481 T16477 prep at,excised
R10093 T16482 T16481 pobj E12.5,at
R10094 T16483 T16482 cc and,E12.5
R10095 T16484 T16482 conj E13.5,E12.5
R10096 T16485 T16477 punct ", ",excised
R10097 T16486 T16477 advmod respectively,excised
R10098 T16487 T16477 punct ", ",excised
R10099 T16488 T16477 conj washed,excised
R10100 T16489 T16488 prep in,washed
R10101 T16490 T16489 pobj PBS,in
R10102 T16491 T16488 cc and,washed
R10103 T16492 T16488 conj dissociated,washed
R10104 T16493 T16492 advmod enzymatically,dissociated
R10105 T16494 T16492 prep for,dissociated
R10106 T16495 T16496 nummod 60,min
R10107 T16496 T16494 pobj min,for
R10108 T16497 T16492 prep at,dissociated
R10109 T16498 T16499 nummod 37,°C
R10110 T16499 T16497 pobj °C,at
R10111 T16500 T16477 punct .,excised
R10112 T16502 T16503 det The,buffer
R10113 T16503 T16505 nsubj buffer,comprised
R10114 T16504 T16503 compound digestion,buffer
R10115 T16506 T16507 punct (,μl
R10116 T16507 T16503 parataxis μl,buffer
R10117 T16508 T16507 nummod 150,μl
R10118 T16509 T16507 prep per,μl
R10119 T16510 T16509 pobj liver,per
R10120 T16511 T16507 punct ),μl
R10121 T16512 T16513 nummod 0.6,U
R10122 T16513 T16514 nmod U,dispase
R10123 T16514 T16505 dative dispase,comprised
R10124 T16515 T16516 punct /,ml
R10125 T16516 T16513 prep ml,U
R10126 T16517 T16514 nummod I,dispase
R10127 T16518 T16519 punct (,Roche
R10128 T16519 T16514 parataxis Roche,dispase
R10129 T16520 T16519 punct ),Roche
R10130 T16521 T16505 punct ", ",comprised
R10131 T16522 T16523 nummod 0.1,%
R10132 T16523 T16524 compound %,D
R10133 T16524 T16505 dobj D,comprised
R10134 T16525 T16524 compound collagenase,D
R10135 T16526 T16527 punct (,Roche
R10136 T16527 T16524 parataxis Roche,D
R10137 T16528 T16527 punct ),Roche
R10138 T16529 T16505 punct ", ",comprised
R10139 T16530 T16531 nummod 10,U
R10140 T16531 T16532 compound U,DNase
R10141 T16532 T16505 dobj DNase,comprised
R10142 T16533 T16534 punct (,Roche
R10143 T16534 T16532 parataxis Roche,DNase
R10144 T16535 T16534 punct ),Roche
R10145 T16536 T16505 punct ", ",comprised
R10146 T16537 T16505 cc and,comprised
R10147 T16538 T16539 nummod 20,%
R10148 T16539 T16540 compound %,FCS
R10149 T16540 T16505 conj FCS,comprised
R10150 T16541 T16540 prep in,FCS
R10151 T16542 T16541 pobj PBS,in
R10152 T16543 T16505 punct .,comprised
R10153 T16545 T16546 nmod X,medium
R10154 T16546 T16550 nsubjpass medium,added
R10155 T16547 T16546 punct -,medium
R10156 T16548 T16546 nmod Vivo,medium
R10157 T16549 T16546 nummod 15,medium
R10158 T16551 T16552 punct (,Cambrex
R10159 T16552 T16546 parataxis Cambrex,medium
R10160 T16553 T16552 punct ", ",Cambrex
R10161 T16554 T16555 compound East,Rutherford
R10162 T16555 T16552 npadvmod Rutherford,Cambrex
R10163 T16556 T16552 punct ", ",Cambrex
R10164 T16557 T16552 npadvmod USA,Cambrex
R10165 T16558 T16552 punct ),Cambrex
R10166 T16559 T16550 auxpass was,added
R10167 T16560 T16550 prep to,added
R10168 T16561 T16562 det the,suspension
R10169 T16562 T16560 pobj suspension,to
R10170 T16563 T16562 amod resulting,suspension
R10171 T16564 T16562 compound cell,suspension
R10172 T16565 T16550 punct ", ",added
R10173 T16566 T16550 cc and,added
R10174 T16567 T16568 prep after,resuspended
R10175 T16568 T16550 conj resuspended,added
R10176 T16569 T16567 pobj centrifugation,after
R10177 T16570 T16571 punct (,g
R10178 T16571 T16569 parataxis g,centrifugation
R10179 T16572 T16571 nummod 200,g
R10180 T16573 T16571 punct ×,g
R10181 T16574 T16571 punct ;,g
R10182 T16575 T16576 nummod 3,min
R10183 T16576 T16571 npadvmod min,g
R10184 T16577 T16571 punct ),g
R10185 T16578 T16568 nsubjpass cells,resuspended
R10186 T16579 T16568 auxpass were,resuspended
R10187 T16580 T16568 prep in,resuspended
R10188 T16581 T16582 nmod X,Vivo
R10189 T16582 T16584 nmod Vivo,medium
R10190 T16583 T16582 punct -,Vivo
R10191 T16584 T16580 pobj medium,in
R10192 T16585 T16582 nummod 15,Vivo
R10193 T16586 T16584 acl supplemented,medium
R10194 T16587 T16586 prep with,supplemented
R10195 T16588 T16589 nummod 50,ng
R10196 T16589 T16590 nmod ng,factor
R10197 T16590 T16587 pobj factor,with
R10198 T16591 T16592 punct /,ml
R10199 T16592 T16589 prep ml,ng
R10200 T16593 T16594 compound macrophage,colony
R10201 T16594 T16595 npadvmod colony,stimulating
R10202 T16595 T16590 amod stimulating,factor
R10203 T16596 T16595 punct -,stimulating
R10204 T16597 T16598 punct (,Aldrich
R10205 T16598 T16590 parataxis Aldrich,factor
R10206 T16599 T16600 compound M,CSF
R10207 T16600 T16598 dep CSF,Aldrich
R10208 T16601 T16600 punct -,CSF
R10209 T16602 T16598 punct ;,Aldrich
R10210 T16603 T16598 compound Sigma,Aldrich
R10211 T16604 T16598 punct -,Aldrich
R10212 T16605 T16598 punct ", ",Aldrich
R10213 T16606 T16607 compound St.,Louis
R10214 T16607 T16598 npadvmod Louis,Aldrich
R10215 T16608 T16598 punct ", ",Aldrich
R10216 T16609 T16598 npadvmod USA,Aldrich
R10217 T16610 T16598 punct ),Aldrich
R10218 T16611 T16568 cc and,resuspended
R10219 T16612 T16568 conj cultured,resuspended
R10220 T16613 T16612 prep on,cultured
R10221 T16614 T16615 amod non-treated,culture
R10222 T16615 T16618 compound culture,dishes
R10223 T16616 T16615 compound tissue,culture
R10224 T16617 T16615 punct -,culture
R10225 T16618 T16613 pobj dishes,on
R10226 T16619 T16612 prep at,cultured
R10227 T16620 T16621 nummod 37,°C
R10228 T16621 T16619 pobj °C,at
R10229 T16622 T16612 prep with,cultured
R10230 T16623 T16624 nummod 5,%
R10231 T16624 T16625 compound %,CO2
R10232 T16625 T16622 pobj CO2,with
R10233 T16626 T16568 punct .,resuspended
R10234 T16628 T16629 det Every,day
R10235 T16629 T16633 npadvmod day,changed
R10236 T16630 T16629 amod second,day
R10237 T16631 T16630 cc or,second
R10238 T16632 T16630 conj third,second
R10239 T16634 T16635 det the,medium
R10240 T16635 T16633 nsubjpass medium,changed
R10241 T16636 T16633 auxpass was,changed
R10242 T16637 T16633 prep by,changed
R10243 T16638 T16637 pobj centrifugation,by
R10244 T16639 T16633 punct .,changed
R10245 T16641 T16642 prep Following,cultured
R10246 T16643 T16641 pobj withdrawal,Following
R10247 T16644 T16643 prep of,withdrawal
R10248 T16645 T16646 compound M,CSF
R10249 T16646 T16644 pobj CSF,of
R10250 T16647 T16646 punct -,CSF
R10251 T16648 T16643 prep on,withdrawal
R10252 T16649 T16648 pobj day,on
R10253 T16650 T16649 nummod 6,day
R10254 T16651 T16649 prep after,day
R10255 T16652 T16651 pobj excision,after
R10256 T16653 T16642 punct ", ",cultured
R10257 T16654 T16655 amod adherent,cells
R10258 T16655 T16642 nsubjpass cells,cultured
R10259 T16656 T16642 auxpass were,cultured
R10260 T16657 T16642 prep for,cultured
R10261 T16658 T16659 det an,h
R10262 T16659 T16657 pobj h,for
R10263 T16660 T16659 amod additional,h
R10264 T16661 T16662 compound 24,48
R10265 T16662 T16659 nummod 48,h
R10266 T16663 T16662 punct -,48
R10267 T16664 T16642 prep in,cultured
R10268 T16665 T16666 nmod X,Vivo
R10269 T16666 T16668 nmod Vivo,medium
R10270 T16667 T16666 punct -,Vivo
R10271 T16668 T16664 pobj medium,in
R10272 T16669 T16666 nummod 15,Vivo
R10273 T16670 T16642 punct .,cultured

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T16445 21-26 UBERON:0002107 denotes liver
T16446 35-46 CL:0000235 denotes macrophages
T16447 61-67 UBERON:0002107 denotes livers
T16448 86-93 UBERON:0000922 denotes embryos
T16449 158-171 CHEBI_GO_SO_EXT:enzyme denotes enzymatically
T16450 206-212 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T16451 225-230 UBERON:0002107 denotes liver
T16452 303-308 GO_EXT:0004536 denotes DNase
T16453 414-418 CL_GO_EXT:cell denotes cell
T16454 473-478 CL_GO_EXT:cell denotes cells
T16455 543-553 CL:0000235 denotes macrophage
T16456 543-579 PR_EXT:000005930 denotes macrophage colony-stimulating factor
T16457 581-582 CL:0000235 denotes M
T16458 581-586 PR_EXT:000005930 denotes M-CSF
T16459 647-653 UBERON:0000479 denotes tissue
T16460 685-688 CHEBI:16526 denotes CO2
T16461 782-787 PR_EXT:000005930 denotes M-CSF
T16462 813-821 GO_PATO_EXT:biological_adhesion_or_attachedness denotes adherent
T16463 822-827 CL_GO_EXT:cell denotes cells

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T16433 21-26 UBERON:0002107 denotes liver
T16434 35-46 CL:0000235 denotes macrophages
T16435 61-67 UBERON:0002107 denotes livers
T16436 86-93 UBERON:0000922 denotes embryos
T16437 225-230 UBERON:0002107 denotes liver
T16438 543-553 CL:0000235 denotes macrophage
T16439 543-579 PR:000005930 denotes macrophage colony-stimulating factor
T16440 581-582 CL:0000235 denotes M
T16441 581-586 PR:000005930 denotes M-CSF
T16442 647-653 UBERON:0000479 denotes tissue
T16443 685-688 CHEBI:16526 denotes CO2
T16444 782-787 PR:000005930 denotes M-CSF