PMC:549712 / 14024-15845 JSONTXT

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A similar delay in tissue differentiation of Ptdsr -/- embryos was found in the kidneys (Figure 3h). Kidneys from Ptdsr+/+ embryos were well developed at E16.5, showing terminally differentiated glomeruli with Bowman's capsule and collecting tubules lined with cuboidal epithelial cells (Figure 3b). In contrast, Ptdsr-deficient kidneys had only primitive glomeruli at E16.5, and collecting tubules were less well-developed. Instead, a large amount of undifferentiated mesenchyme was present in Ptdsr -/- kidneys (Figure 3h). A delay in tissue differentiation was also found in the intestine at this stage of development. Ptdsr -/- embryos displayed improperly developed villi and an underdeveloped or absent submucosa (Figure 3i). In wild-type embryos (Figure 3c), intestinal cellular differentiation was already highly organized, with intramural ganglion cells between the external and internal muscular layers. 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