PMC:5487955 / 3772-4697 JSONTXT

{"project":"2_test","denotations":[{"id":"28445435-19305911-69481143","span":{"begin":132,"end":134},"obj":"19305911"},{"id":"28445435-20408521-69481144","span":{"begin":135,"end":137},"obj":"20408521"}],"text":"2.2. Microarray Fabrication\nMicroarrays of 10,000 20-mer peptides were produced by Arizona State University as previously reported [14,17]. Briefly, 10,000 distinct polypeptide sequences were spotted in duplicate onto a glass slide possessing an amino-silane surface coating. Each polypeptide was 20 residues in length, with 17 positions randomly chosen from a set of 19 amino acids (excluding cysteine). The C-terminal 3 positions of each peptide was a glycine–serine–cysteine (GSC) linker, which was used for conjugating peptides to amino-silane surfaces via a maleimide linker, sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC; Pierce, Rockford, IL, USA). The array printing was performed by using a Telechem Nanoprint60 (Arrayit, Sunnyvale, CA, USA) hat spotted approximately 500 pL of 1 mg/mL peptide per feature on glass slides with 48 Telechem series SMP2-style 946 titanium pins (Arrayit)."}