PMC:548130 / 8089-9705 JSONTXT

{"project":"2_test","denotations":[{"id":"15673474-11241407-8410893","span":{"begin":549,"end":550},"obj":"11241407"},{"id":"15673474-10471054-8410894","span":{"begin":551,"end":552},"obj":"10471054"},{"id":"15673474-15350357-8410895","span":{"begin":1613,"end":1614},"obj":"15350357"}],"text":"LDMAS application in identification of LOH markers associated with persistence / progression of cervical intraepithelial neoplasia\nWe divided the CIN groups into disease free indicating cases that become CIN free after treatment, and disease persistence/progression indicating cases that develop show progression or persistence of CIN despite treatment. We used LDMAS to retrospectively examine the prognostic value of LOH at 12 microsatellite markers including 10 from 3p14, 3p22-21, 6p21 and 11q23 which are frequently deleted in cervical cancer [3,4], in 164 cases of CIN lesions using archival cytological/histological specimens. LOH was further correlated with high risk HPV infection.\nInitially MRES was used to automatically parse 4300 patient records and extract clinico-pathological data including age, diagnosis, method of treatment and treatment response during follow up. Out of those, 164 cases with follow up of 3 or more years were chosen for the study and their clinico-pathological information was imported into LDAS. Initially, 71 out of the 164 selected cases were examined for LOH using 12 fluorescent microsatellite markers ran on ABI377 DNA Sequencer. LDAS was then used to identify the microsatellite markers for which LOH was significantly associated with disease persistence/progression of CIN using two tailed student t-test. Figure 2 generated using LDAS shows that microsatellite markers D3S1300 (3p14.2), D3S1260 (3p22.2), D11S35 (11q22.1) and D11S528 (11q23.3) have the highest LOH in CIN lesions displaying persistence/progression than those who were disease free during follow up [5]."}