PMC:5477810 / 37726-38849 JSONTXT

{"project":"CucurLitBase-testset_Gene","denotations":[{"id":"T106","span":{"begin":15,"end":24},"obj":"ClMPK1"},{"id":"T107","span":{"begin":44,"end":50},"obj":"ClMPK7"},{"id":"T108","span":{"begin":55,"end":66},"obj":"ClMKK2-2"},{"id":"T109","span":{"begin":217,"end":223},"obj":"ClMPK7"},{"id":"T110","span":{"begin":225,"end":236},"obj":"ClMKK2-2"},{"id":"T111","span":{"begin":712,"end":721},"obj":"ClMPK1"},{"id":"T112","span":{"begin":744,"end":750},"obj":"ClMPK7"},{"id":"T113","span":{"begin":1089,"end":1099},"obj":"ClMKK"}],"text":"Fig. 9 Effects of ClMPK1, ClMPK4-2, ClMAK6, ClMPK7 and ClMKK2-2 on systemic resistance to B. cinerea and the expression of defense-related genes. Agrobacteria harboring constructs containing ClMPK1, ClMPK4-2, ClMPK6, ClMPK7, ClMKK2-2 or eGFP (a negative control) were infiltrated into one half of 4-week-old N. benthamiana leaves and the agroinfiltrated leaves were collected for disease assays with B. cinerea. Disease assays were performed by dropping 5 μL of spore suspension (1 × 105 spores/mL) onto the opposite half of the leaves at 2 days after agroinfiltration. Photos for disease phenotype a were taken and lesion sizes b were measured at 4 days after inoculation. c Expression of defense-related genes in ClMPK1-, ClMPK4-2-, ClMPK6-, ClMPK7- and ClMKK2-2-transiently expressed leaves. Leaf samples were collected at 24 h after agroinfiltration and relative expressions are shown as folds of the actin transcript level. Data presented in b and c are the means ± SD from three independent experiments and ** on the columns indicate significant difference at p ≤ 0.05 between ClMPK/ClMKK- and eGFP-infiltrated plants"}

{"project":"CucurLitBase-testset_BFT","denotations":[{"id":"T34","span":{"begin":623,"end":628},"obj":"BFT:1.1 size"},{"id":"T148","span":{"begin":15,"end":24},"obj":"ClMPK1"},{"id":"T149","span":{"begin":44,"end":50},"obj":"ClMPK7"},{"id":"T150","span":{"begin":55,"end":66},"obj":"ClMKK2-2"},{"id":"T151","span":{"begin":217,"end":223},"obj":"ClMPK7"},{"id":"T152","span":{"begin":225,"end":236},"obj":"ClMKK2-2"},{"id":"T153","span":{"begin":712,"end":721},"obj":"ClMPK1"},{"id":"T154","span":{"begin":744,"end":750},"obj":"ClMPK7"},{"id":"T155","span":{"begin":1089,"end":1099},"obj":"ClMKK"}],"text":"Fig. 9 Effects of ClMPK1, ClMPK4-2, ClMAK6, ClMPK7 and ClMKK2-2 on systemic resistance to B. cinerea and the expression of defense-related genes. Agrobacteria harboring constructs containing ClMPK1, ClMPK4-2, ClMPK6, ClMPK7, ClMKK2-2 or eGFP (a negative control) were infiltrated into one half of 4-week-old N. benthamiana leaves and the agroinfiltrated leaves were collected for disease assays with B. cinerea. Disease assays were performed by dropping 5 μL of spore suspension (1 × 105 spores/mL) onto the opposite half of the leaves at 2 days after agroinfiltration. Photos for disease phenotype a were taken and lesion sizes b were measured at 4 days after inoculation. c Expression of defense-related genes in ClMPK1-, ClMPK4-2-, ClMPK6-, ClMPK7- and ClMKK2-2-transiently expressed leaves. Leaf samples were collected at 24 h after agroinfiltration and relative expressions are shown as folds of the actin transcript level. Data presented in b and c are the means ± SD from three independent experiments and ** on the columns indicate significant difference at p ≤ 0.05 between ClMPK/ClMKK- and eGFP-infiltrated plants"}