PMC:5400024 / 4455-5963 JSONTXT

{"project":"0_colil","denotations":[{"id":"28082471-24631044-6923","span":{"begin":274,"end":276},"obj":"24631044"},{"id":"28082471-19059112-6924","span":{"begin":533,"end":534},"obj":"19059112"},{"id":"28082471-24631044-6925","span":{"begin":536,"end":538},"obj":"24631044"},{"id":"28082471-10515395-6926","span":{"begin":1094,"end":1096},"obj":"10515395"}],"text":"Ex vivo lung perfusion protocol\nAdult donor lungs deemed unsuitable for lung transplant by all five UK lung transplant centres but meeting strict EVLP criteria were included in the study (Supplementary Table S1), the results of which were previously published by our group [16]. Lungs were procured in a routine fashion and transported to our institution. The EVLP assessment followed a standardized acellular protocol using the Toronto technique with a closed left atrium and reduced perfusate flow, previously described in detail [3, 16]. Transplant suitability was assessed hourly during perfusion. Lungs meeting transplant criteria (Supplementary Table S2) at two consecutive time points were cooled and transplanted. Lungs deemed to have futile prospects for improvement were taken off the circuit and discarded. Two transplanted and five non-transplanted lungs were perfused ≥5h before a transplant decision was made.\n\nSample collection and processing\nA research BAL was performed for all lungs by wedging an adult bronchoscope in a subsegmental bronchus of the right or left lower lobe [17]. Saline (40 ml) was instilled through the suction channel followed by gentle aspiration and sample collection prior to commencing ventilation at the beginning of EVLP. This process was repeated in the same lobe but in a different subsegmental bronchus before disconnecting the ventilation at the end of perfusion. In addition, hourly perfusate samples of 2.5 ml were collected until the assessment was stopped."}

{"project":"2_test","denotations":[{"id":"28082471-24631044-28905089","span":{"begin":274,"end":276},"obj":"24631044"},{"id":"28082471-19059112-28905090","span":{"begin":533,"end":534},"obj":"19059112"},{"id":"28082471-24631044-28905091","span":{"begin":536,"end":538},"obj":"24631044"},{"id":"28082471-10515395-28905092","span":{"begin":1094,"end":1096},"obj":"10515395"}],"text":"Ex vivo lung perfusion protocol\nAdult donor lungs deemed unsuitable for lung transplant by all five UK lung transplant centres but meeting strict EVLP criteria were included in the study (Supplementary Table S1), the results of which were previously published by our group [16]. Lungs were procured in a routine fashion and transported to our institution. The EVLP assessment followed a standardized acellular protocol using the Toronto technique with a closed left atrium and reduced perfusate flow, previously described in detail [3, 16]. Transplant suitability was assessed hourly during perfusion. Lungs meeting transplant criteria (Supplementary Table S2) at two consecutive time points were cooled and transplanted. Lungs deemed to have futile prospects for improvement were taken off the circuit and discarded. Two transplanted and five non-transplanted lungs were perfused ≥5h before a transplant decision was made.\n\nSample collection and processing\nA research BAL was performed for all lungs by wedging an adult bronchoscope in a subsegmental bronchus of the right or left lower lobe [17]. Saline (40 ml) was instilled through the suction channel followed by gentle aspiration and sample collection prior to commencing ventilation at the beginning of EVLP. This process was repeated in the same lobe but in a different subsegmental bronchus before disconnecting the ventilation at the end of perfusion. In addition, hourly perfusate samples of 2.5 ml were collected until the assessment was stopped."}

{"project":"MyTest","denotations":[{"id":"28082471-24631044-28905089","span":{"begin":274,"end":276},"obj":"24631044"},{"id":"28082471-19059112-28905090","span":{"begin":533,"end":534},"obj":"19059112"},{"id":"28082471-24631044-28905091","span":{"begin":536,"end":538},"obj":"24631044"},{"id":"28082471-10515395-28905092","span":{"begin":1094,"end":1096},"obj":"10515395"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"text":"Ex vivo lung perfusion protocol\nAdult donor lungs deemed unsuitable for lung transplant by all five UK lung transplant centres but meeting strict EVLP criteria were included in the study (Supplementary Table S1), the results of which were previously published by our group [16]. Lungs were procured in a routine fashion and transported to our institution. The EVLP assessment followed a standardized acellular protocol using the Toronto technique with a closed left atrium and reduced perfusate flow, previously described in detail [3, 16]. Transplant suitability was assessed hourly during perfusion. Lungs meeting transplant criteria (Supplementary Table S2) at two consecutive time points were cooled and transplanted. Lungs deemed to have futile prospects for improvement were taken off the circuit and discarded. Two transplanted and five non-transplanted lungs were perfused ≥5h before a transplant decision was made.\n\nSample collection and processing\nA research BAL was performed for all lungs by wedging an adult bronchoscope in a subsegmental bronchus of the right or left lower lobe [17]. Saline (40 ml) was instilled through the suction channel followed by gentle aspiration and sample collection prior to commencing ventilation at the beginning of EVLP. This process was repeated in the same lobe but in a different subsegmental bronchus before disconnecting the ventilation at the end of perfusion. In addition, hourly perfusate samples of 2.5 ml were collected until the assessment was stopped."}

{"project":"testtesttest","denotations":[{"id":"T38","span":{"begin":8,"end":12},"obj":"Body_part"},{"id":"T39","span":{"begin":44,"end":49},"obj":"Body_part"},{"id":"T40","span":{"begin":72,"end":76},"obj":"Body_part"},{"id":"T41","span":{"begin":103,"end":107},"obj":"Body_part"},{"id":"T42","span":{"begin":279,"end":284},"obj":"Body_part"},{"id":"T43","span":{"begin":461,"end":472},"obj":"Body_part"},{"id":"T44","span":{"begin":602,"end":607},"obj":"Body_part"},{"id":"T45","span":{"begin":722,"end":727},"obj":"Body_part"},{"id":"T46","span":{"begin":787,"end":790},"obj":"Body_part"},{"id":"T47","span":{"begin":861,"end":866},"obj":"Body_part"},{"id":"T48","span":{"begin":995,"end":1000},"obj":"Body_part"},{"id":"T49","span":{"begin":1039,"end":1060},"obj":"Body_part"},{"id":"T50","span":{"begin":1077,"end":1092},"obj":"Body_part"},{"id":"T51","span":{"begin":1271,"end":1278},"obj":"Body_part"},{"id":"T52","span":{"begin":1328,"end":1349},"obj":"Body_part"}],"attributes":[{"id":"A38","pred":"uberon_id","subj":"T38","obj":"http://purl.obolibrary.org/obo/UBERON_0002048"},{"id":"A39","pred":"uberon_id","subj":"T39","obj":"http://purl.obolibrary.org/obo/UBERON_0000170"},{"id":"A40","pred":"uberon_id","subj":"T40","obj":"http://purl.obolibrary.org/obo/UBERON_0002048"},{"id":"A41","pred":"uberon_id","subj":"T41","obj":"http://purl.obolibrary.org/obo/UBERON_0002048"},{"id":"A42","pred":"uberon_id","subj":"T42","obj":"http://purl.obolibrary.org/obo/UBERON_0000170"},{"id":"A43","pred":"uberon_id","subj":"T43","obj":"http://purl.obolibrary.org/obo/UBERON_0002079"},{"id":"A44","pred":"uberon_id","subj":"T44","obj":"http://purl.obolibrary.org/obo/UBERON_0000170"},{"id":"A45","pred":"uberon_id","subj":"T45","obj":"http://purl.obolibrary.org/obo/UBERON_0000170"},{"id":"A46","pred":"uberon_id","subj":"T46","obj":"http://purl.obolibrary.org/obo/UBERON_0034754"},{"id":"A47","pred":"uberon_id","subj":"T47","obj":"http://purl.obolibrary.org/obo/UBERON_0000170"},{"id":"A48","pred":"uberon_id","subj":"T48","obj":"http://purl.obolibrary.org/obo/UBERON_0000170"},{"id":"A49","pred":"uberon_id","subj":"T49","obj":"http://purl.obolibrary.org/obo/UBERON_8600009"},{"id":"A50","pred":"uberon_id","subj":"T50","obj":"http://purl.obolibrary.org/obo/UBERON_0008953"},{"id":"A51","pred":"uberon_id","subj":"T51","obj":"http://purl.obolibrary.org/obo/UBERON_0004529"},{"id":"A52","pred":"uberon_id","subj":"T52","obj":"http://purl.obolibrary.org/obo/UBERON_8600009"}],"text":"Ex vivo lung perfusion protocol\nAdult donor lungs deemed unsuitable for lung transplant by all five UK lung transplant centres but meeting strict EVLP criteria were included in the study (Supplementary Table S1), the results of which were previously published by our group [16]. Lungs were procured in a routine fashion and transported to our institution. The EVLP assessment followed a standardized acellular protocol using the Toronto technique with a closed left atrium and reduced perfusate flow, previously described in detail [3, 16]. Transplant suitability was assessed hourly during perfusion. Lungs meeting transplant criteria (Supplementary Table S2) at two consecutive time points were cooled and transplanted. Lungs deemed to have futile prospects for improvement were taken off the circuit and discarded. Two transplanted and five non-transplanted lungs were perfused ≥5h before a transplant decision was made.\n\nSample collection and processing\nA research BAL was performed for all lungs by wedging an adult bronchoscope in a subsegmental bronchus of the right or left lower lobe [17]. Saline (40 ml) was instilled through the suction channel followed by gentle aspiration and sample collection prior to commencing ventilation at the beginning of EVLP. This process was repeated in the same lobe but in a different subsegmental bronchus before disconnecting the ventilation at the end of perfusion. In addition, hourly perfusate samples of 2.5 ml were collected until the assessment was stopped."}