PMC:5400024 / 22381-23241 JSONTXT

{"project":"testtesttest","denotations":[{"id":"T154","span":{"begin":74,"end":80},"obj":"Body_part"},{"id":"T155","span":{"begin":140,"end":145},"obj":"Body_part"},{"id":"T156","span":{"begin":262,"end":267},"obj":"Body_part"},{"id":"T157","span":{"begin":643,"end":648},"obj":"Body_part"},{"id":"T158","span":{"begin":841,"end":847},"obj":"Body_part"}],"attributes":[{"id":"A154","pred":"uberon_id","subj":"T154","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"A155","pred":"uberon_id","subj":"T155","obj":"http://purl.obolibrary.org/obo/UBERON_0000170"},{"id":"A156","pred":"uberon_id","subj":"T156","obj":"http://purl.obolibrary.org/obo/UBERON_0000170"},{"id":"A157","pred":"uberon_id","subj":"T157","obj":"http://purl.obolibrary.org/obo/UBERON_0000170"},{"id":"A158","pred":"uberon_id","subj":"T158","obj":"http://purl.obolibrary.org/obo/UBERON_0000117"},{"id":"A159","pred":"uberon_id","subj":"T158","obj":"http://purl.obolibrary.org/obo/UBERON_0001005"}],"text":"In this proof-of-concept study, the feasibility of using inflammation and tissue injury markers in both perfusate and BALF from human donor lungs undergoing EVLP to validate the decision to use or decline them for clinical transplant was evaluated. All 16 donor lungs in this study were assessed consecutively with intent for transplant following a strict protocol with predefined criteria for EVLP and transplant suitability to reduce the risk of bias in decision making. All protein analyses were done retrospectively and had no impact on the transplant decision. Our results demonstrate a difference in protein expression in perfusate from lungs that were transplanted compared with those that were declined. This difference became more apparent for each hour of perfusion, and BALF samples showed a comparable but weaker signal from the airway compartment."}