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PMC:539297 / 35000-35675 JSONTXT

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craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T7609 333-340 CHEBI:33893 denotes Reagent
T7610 368-378 GO:0019835 denotes cell lysis

craft-sa-dev

Id Subject Object Predicate Lexical cue
T7619 0-3 NN denotes ATP
T7620 4-15 NN denotes measurement
T7621 15-151 sentence denotes ATP was measured by using the CellTiter-GLO® Luminescent Cell Viability kit (Promega), according to the manufacturer's recommendations.
T7622 16-19 NN denotes ATP
T7623 24-32 VBN denotes measured
T7624 20-23 VBD denotes was
T7625 33-35 IN denotes by
T7626 36-41 VBG denotes using
T7627 42-45 DT denotes the
T7628 88-91 NN denotes kit
T7629 46-55 NNP denotes CellTiter
T7630 56-60 NNP denotes GLO®
T7631 55-56 HYPH denotes -
T7632 61-72 JJ denotes Luminescent
T7633 73-77 NN denotes Cell
T7634 78-87 NN denotes Viability
T7635 92-93 -LRB- denotes (
T7636 93-100 NNP denotes Promega
T7637 100-101 -RRB- denotes )
T7638 101-103 , denotes ,
T7639 103-112 VBG denotes according
T7640 113-115 IN denotes to
T7641 116-119 DT denotes the
T7642 120-132 NN denotes manufacturer
T7643 135-150 NNS denotes recommendations
T7644 132-134 POS denotes 's
T7645 150-151 . denotes .
T7646 151-298 sentence denotes Briefly, cells were plated in a black opaque-walled 96 well plate (Packard Bioscience) at a density of 20000/well and incubated at 33°C overnight.
T7647 152-159 RB denotes Briefly
T7648 172-178 VBN denotes plated
T7649 159-161 , denotes ,
T7650 161-166 NNS denotes cells
T7651 167-171 VBD denotes were
T7652 179-181 IN denotes in
T7653 182-183 DT denotes a
T7654 212-217 NN denotes plate
T7655 184-189 JJ denotes black
T7656 190-196 JJ denotes opaque
T7657 197-203 JJ denotes walled
T7658 196-197 HYPH denotes -
T7659 204-206 CD denotes 96
T7660 207-211 NN denotes well
T7661 218-219 -LRB- denotes (
T7662 227-237 NNP denotes Bioscience
T7663 219-226 NNP denotes Packard
T7664 237-238 -RRB- denotes )
T7665 239-241 IN denotes at
T7666 242-243 DT denotes a
T7667 244-251 NN denotes density
T7668 252-254 IN denotes of
T7669 255-260 CD denotes 20000
T7670 260-261 SYM denotes /
T7671 261-265 NN denotes well
T7672 266-269 CC denotes and
T7673 270-279 VBN denotes incubated
T7674 280-282 IN denotes at
T7675 283-285 CD denotes 33
T7676 285-287 NN denotes °C
T7677 288-297 RB denotes overnight
T7678 297-298 . denotes .
T7679 298-411 sentence denotes The following day, CellTiter-GLO® Reagent was added to each well and cell lysis was induced by mixing 2 minutes.
T7680 299-302 DT denotes The
T7681 313-316 NN denotes day
T7682 303-312 JJ denotes following
T7683 345-350 VBN denotes added
T7684 316-318 , denotes ,
T7685 318-327 NNP denotes CellTiter
T7686 328-332 NNP denotes GLO®
T7687 327-328 HYPH denotes -
T7688 333-340 NN denotes Reagent
T7689 341-344 VBD denotes was
T7690 351-353 IN denotes to
T7691 354-358 DT denotes each
T7692 359-363 NN denotes well
T7693 364-367 CC denotes and
T7694 368-372 NN denotes cell
T7695 373-378 NN denotes lysis
T7696 383-390 VBN denotes induced
T7697 379-382 VBD denotes was
T7698 391-393 IN denotes by
T7699 394-400 VBG denotes mixing
T7700 401-402 CD denotes 2
T7701 403-410 NNS denotes minutes
T7702 410-411 . denotes .
T7703 411-465 sentence denotes An ATP standard curve was prepared in the same plate.
T7704 412-414 DT denotes An
T7705 428-433 NN denotes curve
T7706 415-418 NN denotes ATP
T7707 419-427 JJ denotes standard
T7708 438-446 VBN denotes prepared
T7709 434-437 VBD denotes was
T7710 447-449 IN denotes in
T7711 450-453 DT denotes the
T7712 459-464 NN denotes plate
T7713 454-458 JJ denotes same
T7714 464-465 . denotes .
T7715 465-675 sentence denotes Before recording luminescence with a microplate luminometer (MicroLumat Plus LB 96V, Berthold Techonologies), the plate was dark adapted for 10 minutes at room temperature to stabilize the luminescence signal.
T7716 466-472 IN denotes Before
T7717 595-602 VBN denotes adapted
T7718 473-482 VBG denotes recording
T7719 483-495 NN denotes luminescence
T7720 496-500 IN denotes with
T7721 501-502 DT denotes a
T7722 514-525 NN denotes luminometer
T7723 503-513 NN denotes microplate
T7724 526-527 -LRB- denotes (
T7725 527-537 NNP denotes MicroLumat
T7726 546-549 NNP denotes 96V
T7727 538-542 NNP denotes Plus
T7728 543-545 NNP denotes LB
T7729 549-551 , denotes ,
T7730 560-573 NNP denotes Techonologies
T7731 551-559 NNP denotes Berthold
T7732 573-574 -RRB- denotes )
T7733 574-576 , denotes ,
T7734 576-579 DT denotes the
T7735 580-585 NN denotes plate
T7736 586-589 VBD denotes was
T7737 590-594 NN denotes dark
T7738 603-606 IN denotes for
T7739 607-609 CD denotes 10
T7740 610-617 NNS denotes minutes
T7741 618-620 IN denotes at
T7742 621-625 NN denotes room
T7743 626-637 NN denotes temperature
T7744 638-640 TO denotes to
T7745 641-650 VB denotes stabilize
T7746 651-654 DT denotes the
T7747 668-674 NN denotes signal
T7748 655-667 NN denotes luminescence
T7749 674-675 . denotes .
R4853 T7619 T7620 compound ATP,measurement
R4854 T7622 T7623 nsubjpass ATP,measured
R4855 T7624 T7623 auxpass was,measured
R4856 T7625 T7623 prep by,measured
R4857 T7626 T7625 pcomp using,by
R4858 T7627 T7628 det the,kit
R4859 T7628 T7626 dobj kit,using
R4860 T7629 T7630 nmod CellTiter,GLO®
R4861 T7630 T7628 nmod GLO®,kit
R4862 T7631 T7630 punct -,GLO®
R4863 T7632 T7628 amod Luminescent,kit
R4864 T7633 T7634 compound Cell,Viability
R4865 T7634 T7628 compound Viability,kit
R4866 T7635 T7636 punct (,Promega
R4867 T7636 T7628 parataxis Promega,kit
R4868 T7637 T7636 punct ),Promega
R4869 T7638 T7623 punct ", ",measured
R4870 T7639 T7623 prep according,measured
R4871 T7640 T7639 prep to,according
R4872 T7641 T7642 det the,manufacturer
R4873 T7642 T7643 poss manufacturer,recommendations
R4874 T7643 T7640 pobj recommendations,to
R4875 T7644 T7642 case 's,manufacturer
R4876 T7645 T7623 punct .,measured
R4877 T7647 T7648 advmod Briefly,plated
R4878 T7649 T7648 punct ", ",plated
R4879 T7650 T7648 nsubjpass cells,plated
R4880 T7651 T7648 auxpass were,plated
R4881 T7652 T7648 prep in,plated
R4882 T7653 T7654 det a,plate
R4883 T7654 T7652 pobj plate,in
R4884 T7655 T7654 amod black,plate
R4885 T7656 T7657 amod opaque,walled
R4886 T7657 T7654 amod walled,plate
R4887 T7658 T7657 punct -,walled
R4888 T7659 T7660 nummod 96,well
R4889 T7660 T7654 compound well,plate
R4890 T7661 T7662 punct (,Bioscience
R4891 T7662 T7654 parataxis Bioscience,plate
R4892 T7663 T7662 compound Packard,Bioscience
R4893 T7664 T7662 punct ),Bioscience
R4894 T7665 T7648 prep at,plated
R4895 T7666 T7667 det a,density
R4896 T7667 T7665 pobj density,at
R4897 T7668 T7667 prep of,density
R4898 T7669 T7668 pobj 20000,of
R4899 T7670 T7671 punct /,well
R4900 T7671 T7669 prep well,20000
R4901 T7672 T7648 cc and,plated
R4902 T7673 T7648 conj incubated,plated
R4903 T7674 T7673 prep at,incubated
R4904 T7675 T7676 nummod 33,°C
R4905 T7676 T7674 pobj °C,at
R4906 T7677 T7673 advmod overnight,incubated
R4907 T7678 T7648 punct .,plated
R4908 T7680 T7681 det The,day
R4909 T7681 T7683 npadvmod day,added
R4910 T7682 T7681 amod following,day
R4911 T7684 T7683 punct ", ",added
R4912 T7685 T7686 compound CellTiter,GLO®
R4913 T7686 T7688 compound GLO®,Reagent
R4914 T7687 T7686 punct -,GLO®
R4915 T7688 T7683 nsubjpass Reagent,added
R4916 T7689 T7683 auxpass was,added
R4917 T7690 T7683 prep to,added
R4918 T7691 T7692 det each,well
R4919 T7692 T7690 pobj well,to
R4920 T7693 T7683 cc and,added
R4921 T7694 T7695 compound cell,lysis
R4922 T7695 T7696 nsubjpass lysis,induced
R4923 T7696 T7683 conj induced,added
R4924 T7697 T7696 auxpass was,induced
R4925 T7698 T7696 prep by,induced
R4926 T7699 T7698 pcomp mixing,by
R4927 T7700 T7701 nummod 2,minutes
R4928 T7701 T7699 npadvmod minutes,mixing
R4929 T7702 T7696 punct .,induced
R4930 T7704 T7705 det An,curve
R4931 T7705 T7708 nsubjpass curve,prepared
R4932 T7706 T7705 nmod ATP,curve
R4933 T7707 T7705 amod standard,curve
R4934 T7709 T7708 auxpass was,prepared
R4935 T7710 T7708 prep in,prepared
R4936 T7711 T7712 det the,plate
R4937 T7712 T7710 pobj plate,in
R4938 T7713 T7712 amod same,plate
R4939 T7714 T7708 punct .,prepared
R4940 T7716 T7717 prep Before,adapted
R4941 T7718 T7716 pcomp recording,Before
R4942 T7719 T7718 dobj luminescence,recording
R4943 T7720 T7718 prep with,recording
R4944 T7721 T7722 det a,luminometer
R4945 T7722 T7720 pobj luminometer,with
R4946 T7723 T7722 compound microplate,luminometer
R4947 T7724 T7722 punct (,luminometer
R4948 T7725 T7726 compound MicroLumat,96V
R4949 T7726 T7722 appos 96V,luminometer
R4950 T7727 T7726 compound Plus,96V
R4951 T7728 T7726 compound LB,96V
R4952 T7729 T7730 punct ", ",Techonologies
R4953 T7730 T7722 parataxis Techonologies,luminometer
R4954 T7731 T7730 compound Berthold,Techonologies
R4955 T7732 T7730 punct ),Techonologies
R4956 T7733 T7717 punct ", ",adapted
R4957 T7734 T7735 det the,plate
R4958 T7735 T7717 nsubjpass plate,adapted
R4959 T7736 T7717 auxpass was,adapted
R4960 T7737 T7717 dep dark,adapted
R4961 T7738 T7717 prep for,adapted
R4962 T7739 T7740 nummod 10,minutes
R4963 T7740 T7738 pobj minutes,for
R4964 T7741 T7717 prep at,adapted
R4965 T7742 T7743 compound room,temperature
R4966 T7743 T7741 pobj temperature,at
R4967 T7744 T7745 aux to,stabilize
R4968 T7745 T7717 advcl stabilize,adapted
R4969 T7746 T7747 det the,signal
R4970 T7747 T7745 dobj signal,stabilize
R4971 T7748 T7747 compound luminescence,signal
R4972 T7749 T7717 punct .,adapted

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T7611 0-3 CHEBI_EXT:ATP denotes ATP
T7612 16-19 CHEBI_EXT:ATP denotes ATP
T7613 73-77 CL_GO_EXT:cell denotes Cell
T7614 161-166 CL_GO_EXT:cell denotes cells
T7615 333-340 CHEBI_EXT:33893 denotes Reagent
T7616 368-372 CL_GO_EXT:cell denotes cell
T7617 368-378 GO:0019835 denotes cell lysis
T7618 415-418 CHEBI_EXT:ATP denotes ATP