Id |
Subject |
Object |
Predicate |
Lexical cue |
T6663 |
0-3 |
IN |
denotes |
For |
T6664 |
31-37 |
VBN |
denotes |
seeded |
T6665 |
4-18 |
NN |
denotes |
immunostaining |
T6666 |
18-20 |
, |
denotes |
, |
T6667 |
20-25 |
NNS |
denotes |
cells |
T6668 |
26-30 |
VBD |
denotes |
were |
T6669 |
38-40 |
IN |
denotes |
at |
T6670 |
41-42 |
DT |
denotes |
a |
T6671 |
43-50 |
NN |
denotes |
density |
T6672 |
51-53 |
IN |
denotes |
of |
T6673 |
54-55 |
CD |
denotes |
3 |
T6674 |
56-57 |
CD |
denotes |
5 |
T6675 |
55-56 |
SYM |
denotes |
– |
T6676 |
60-63 |
CD |
denotes |
104 |
T6677 |
58-59 |
SYM |
denotes |
× |
T6678 |
64-69 |
NNS |
denotes |
cells |
T6679 |
70-73 |
IN |
denotes |
per |
T6680 |
74-78 |
NN |
denotes |
well |
T6681 |
79-81 |
IN |
denotes |
in |
T6682 |
82-83 |
CD |
denotes |
4 |
T6683 |
84-88 |
NN |
denotes |
well |
T6684 |
83-84 |
HYPH |
denotes |
- |
T6685 |
97-103 |
NNS |
denotes |
slides |
T6686 |
89-96 |
NN |
denotes |
chamber |
T6687 |
96-97 |
HYPH |
denotes |
- |
T6688 |
104-107 |
CC |
denotes |
and |
T6689 |
108-113 |
VBN |
denotes |
grown |
T6690 |
114-123 |
RB |
denotes |
overnight |
T6691 |
124-126 |
IN |
denotes |
at |
T6692 |
127-129 |
CD |
denotes |
33 |
T6693 |
129-131 |
NN |
denotes |
°C |
T6694 |
131-133 |
, |
denotes |
, |
T6695 |
133-139 |
IN |
denotes |
unless |
T6696 |
140-149 |
VBN |
denotes |
indicated |
T6697 |
150-159 |
RB |
denotes |
otherwise |
T6698 |
159-160 |
. |
denotes |
. |
T6699 |
160-344 |
sentence |
denotes |
Fixation was with ice-cold 4% formaldehyde in PBS, pH 7.4, for 20 minutes, or with ice-cold methanol/acetone (1:1) for 10 minutes at -20°C followed by air-drying (antibody-dependent). |
T6700 |
161-169 |
NN |
denotes |
Fixation |
T6701 |
170-173 |
VBD |
denotes |
was |
T6702 |
174-178 |
IN |
denotes |
with |
T6703 |
179-182 |
NN |
denotes |
ice |
T6704 |
183-187 |
JJ |
denotes |
cold |
T6705 |
182-183 |
HYPH |
denotes |
- |
T6706 |
191-203 |
NN |
denotes |
formaldehyde |
T6707 |
188-189 |
CD |
denotes |
4 |
T6708 |
189-190 |
NN |
denotes |
% |
T6709 |
204-206 |
IN |
denotes |
in |
T6710 |
207-210 |
NN |
denotes |
PBS |
T6711 |
210-212 |
, |
denotes |
, |
T6712 |
212-214 |
NN |
denotes |
pH |
T6713 |
215-218 |
CD |
denotes |
7.4 |
T6714 |
218-220 |
, |
denotes |
, |
T6715 |
220-223 |
IN |
denotes |
for |
T6716 |
224-226 |
CD |
denotes |
20 |
T6717 |
227-234 |
NNS |
denotes |
minutes |
T6718 |
234-236 |
, |
denotes |
, |
T6719 |
236-238 |
CC |
denotes |
or |
T6720 |
239-243 |
IN |
denotes |
with |
T6721 |
244-247 |
NN |
denotes |
ice |
T6722 |
248-252 |
JJ |
denotes |
cold |
T6723 |
247-248 |
HYPH |
denotes |
- |
T6724 |
262-269 |
NN |
denotes |
acetone |
T6725 |
253-261 |
NN |
denotes |
methanol |
T6726 |
261-262 |
HYPH |
denotes |
/ |
T6727 |
270-271 |
-LRB- |
denotes |
( |
T6728 |
271-272 |
CD |
denotes |
1 |
T6729 |
272-273 |
SYM |
denotes |
: |
T6730 |
273-274 |
CD |
denotes |
1 |
T6731 |
274-275 |
-RRB- |
denotes |
) |
T6732 |
276-279 |
IN |
denotes |
for |
T6733 |
280-282 |
CD |
denotes |
10 |
T6734 |
283-290 |
NNS |
denotes |
minutes |
T6735 |
291-293 |
IN |
denotes |
at |
T6736 |
294-295 |
SYM |
denotes |
- |
T6737 |
295-297 |
CD |
denotes |
20 |
T6738 |
297-299 |
NN |
denotes |
°C |
T6739 |
300-308 |
VBN |
denotes |
followed |
T6740 |
309-311 |
IN |
denotes |
by |
T6741 |
312-315 |
NN |
denotes |
air |
T6742 |
316-322 |
NN |
denotes |
drying |
T6743 |
315-316 |
HYPH |
denotes |
- |
T6744 |
323-324 |
-LRB- |
denotes |
( |
T6745 |
333-342 |
JJ |
denotes |
dependent |
T6746 |
324-332 |
NN |
denotes |
antibody |
T6747 |
332-333 |
HYPH |
denotes |
- |
T6748 |
342-343 |
-RRB- |
denotes |
) |
T6749 |
343-344 |
. |
denotes |
. |
T6750 |
344-763 |
sentence |
denotes |
Cells were washed with PBS at least 2 times, 5 minutes per wash, between each of the following steps of the staining procedure: 0.1 M glycine in PBS for 5 minutes, 0.05% or 0.1% (antibody-dependent) Triton X-100 (Fisher Scientific) in PBS for 5 minutes, 2% bovine serum albumin (BSA) in PBS for 30 minutes, primary antibody diluted in 2% BSA/PBS for 90 minutes, secondary antibody diluted in 2% BSA/PBS for 60 minutes. |
T6751 |
345-350 |
NNS |
denotes |
Cells |
T6752 |
356-362 |
VBN |
denotes |
washed |
T6753 |
351-355 |
VBD |
denotes |
were |
T6754 |
363-367 |
IN |
denotes |
with |
T6755 |
368-371 |
NN |
denotes |
PBS |
T6756 |
372-374 |
RB |
denotes |
at |
T6757 |
381-382 |
CD |
denotes |
2 |
T6758 |
375-380 |
RBS |
denotes |
least |
T6759 |
383-388 |
NNS |
denotes |
times |
T6760 |
388-390 |
, |
denotes |
, |
T6761 |
390-391 |
CD |
denotes |
5 |
T6762 |
392-399 |
NNS |
denotes |
minutes |
T6763 |
400-403 |
IN |
denotes |
per |
T6764 |
404-408 |
NN |
denotes |
wash |
T6765 |
408-410 |
, |
denotes |
, |
T6766 |
410-417 |
IN |
denotes |
between |
T6767 |
418-422 |
DT |
denotes |
each |
T6768 |
423-425 |
IN |
denotes |
of |
T6769 |
426-429 |
DT |
denotes |
the |
T6770 |
440-445 |
NNS |
denotes |
steps |
T6771 |
430-439 |
JJ |
denotes |
following |
T6772 |
446-448 |
IN |
denotes |
of |
T6773 |
449-452 |
DT |
denotes |
the |
T6774 |
462-471 |
NN |
denotes |
procedure |
T6775 |
453-461 |
NN |
denotes |
staining |
T6776 |
471-473 |
: |
denotes |
: |
T6777 |
473-476 |
CD |
denotes |
0.1 |
T6778 |
477-478 |
NN |
denotes |
M |
T6779 |
479-486 |
NN |
denotes |
glycine |
T6780 |
487-489 |
IN |
denotes |
in |
T6781 |
490-493 |
NN |
denotes |
PBS |
T6782 |
494-497 |
IN |
denotes |
for |
T6783 |
498-499 |
CD |
denotes |
5 |
T6784 |
500-507 |
NNS |
denotes |
minutes |
T6785 |
507-509 |
, |
denotes |
, |
T6786 |
509-513 |
CD |
denotes |
0.05 |
T6787 |
513-514 |
NN |
denotes |
% |
T6788 |
551-552 |
NN |
denotes |
X |
T6789 |
515-517 |
CC |
denotes |
or |
T6790 |
518-521 |
CD |
denotes |
0.1 |
T6791 |
521-522 |
NN |
denotes |
% |
T6792 |
523-524 |
-LRB- |
denotes |
( |
T6793 |
524-532 |
NN |
denotes |
antibody |
T6794 |
533-542 |
JJ |
denotes |
dependent |
T6795 |
532-533 |
HYPH |
denotes |
- |
T6796 |
542-543 |
-RRB- |
denotes |
) |
T6797 |
544-550 |
NN |
denotes |
Triton |
T6798 |
552-553 |
HYPH |
denotes |
- |
T6799 |
553-556 |
CD |
denotes |
100 |
T6800 |
557-558 |
-LRB- |
denotes |
( |
T6801 |
565-575 |
NNP |
denotes |
Scientific |
T6802 |
558-564 |
NNP |
denotes |
Fisher |
T6803 |
575-576 |
-RRB- |
denotes |
) |
T6804 |
577-579 |
IN |
denotes |
in |
T6805 |
580-583 |
NN |
denotes |
PBS |
T6806 |
584-587 |
IN |
denotes |
for |
T6807 |
588-589 |
CD |
denotes |
5 |
T6808 |
590-597 |
NNS |
denotes |
minutes |
T6809 |
597-599 |
, |
denotes |
, |
T6810 |
599-600 |
CD |
denotes |
2 |
T6811 |
600-601 |
NN |
denotes |
% |
T6812 |
602-608 |
JJ |
denotes |
bovine |
T6813 |
615-622 |
NN |
denotes |
albumin |
T6814 |
609-614 |
NN |
denotes |
serum |
T6815 |
623-624 |
-LRB- |
denotes |
( |
T6816 |
624-627 |
NN |
denotes |
BSA |
T6817 |
627-628 |
-RRB- |
denotes |
) |
T6818 |
629-631 |
IN |
denotes |
in |
T6819 |
632-635 |
NN |
denotes |
PBS |
T6820 |
636-639 |
IN |
denotes |
for |
T6821 |
640-642 |
CD |
denotes |
30 |
T6822 |
643-650 |
NNS |
denotes |
minutes |
T6823 |
650-652 |
, |
denotes |
, |
T6824 |
652-659 |
JJ |
denotes |
primary |
T6825 |
660-668 |
NN |
denotes |
antibody |
T6826 |
669-676 |
VBN |
denotes |
diluted |
T6827 |
677-679 |
IN |
denotes |
in |
T6828 |
680-681 |
CD |
denotes |
2 |
T6829 |
681-682 |
NN |
denotes |
% |
T6830 |
687-690 |
NN |
denotes |
PBS |
T6831 |
683-686 |
NN |
denotes |
BSA |
T6832 |
686-687 |
HYPH |
denotes |
/ |
T6833 |
691-694 |
IN |
denotes |
for |
T6834 |
695-697 |
CD |
denotes |
90 |
T6835 |
698-705 |
NNS |
denotes |
minutes |
T6836 |
705-707 |
, |
denotes |
, |
T6837 |
707-716 |
JJ |
denotes |
secondary |
T6838 |
717-725 |
NN |
denotes |
antibody |
T6839 |
726-733 |
VBN |
denotes |
diluted |
T6840 |
734-736 |
IN |
denotes |
in |
T6841 |
737-738 |
CD |
denotes |
2 |
T6842 |
738-739 |
NN |
denotes |
% |
T6843 |
744-747 |
NN |
denotes |
PBS |
T6844 |
740-743 |
NN |
denotes |
BSA |
T6845 |
743-744 |
HYPH |
denotes |
/ |
T6846 |
748-751 |
IN |
denotes |
for |
T6847 |
752-754 |
CD |
denotes |
60 |
T6848 |
755-762 |
NNS |
denotes |
minutes |
T6849 |
762-763 |
. |
denotes |
. |
T6850 |
763-817 |
sentence |
denotes |
All incubations were carried out at room temperature. |
T6851 |
764-767 |
DT |
denotes |
All |
T6852 |
768-779 |
NNS |
denotes |
incubations |
T6853 |
785-792 |
VBN |
denotes |
carried |
T6854 |
780-784 |
VBD |
denotes |
were |
T6855 |
793-796 |
RP |
denotes |
out |
T6856 |
797-799 |
IN |
denotes |
at |
T6857 |
800-804 |
NN |
denotes |
room |
T6858 |
805-816 |
NN |
denotes |
temperature |
T6859 |
816-817 |
. |
denotes |
. |
T6860 |
817-1068 |
sentence |
denotes |
Following staining procedures, slides were coverslipped with Vectashield mounting medium (Vector Laboratories) and analyzed on a BioRad Radiance 2100 confocal microscope (Biorad), with identical exposure settings for wild-type and mutant like images. |
T6861 |
818-827 |
VBG |
denotes |
Following |
T6862 |
861-873 |
VBN |
denotes |
coverslipped |
T6863 |
828-836 |
NN |
denotes |
staining |
T6864 |
837-847 |
NNS |
denotes |
procedures |
T6865 |
847-849 |
, |
denotes |
, |
T6866 |
849-855 |
NNS |
denotes |
slides |
T6867 |
856-860 |
VBD |
denotes |
were |
T6868 |
874-878 |
IN |
denotes |
with |
T6869 |
879-890 |
NNP |
denotes |
Vectashield |
T6870 |
900-906 |
NN |
denotes |
medium |
T6871 |
891-899 |
NN |
denotes |
mounting |
T6872 |
907-908 |
-LRB- |
denotes |
( |
T6873 |
915-927 |
NNP |
denotes |
Laboratories |
T6874 |
908-914 |
NNP |
denotes |
Vector |
T6875 |
927-928 |
-RRB- |
denotes |
) |
T6876 |
929-932 |
CC |
denotes |
and |
T6877 |
933-941 |
VBN |
denotes |
analyzed |
T6878 |
942-944 |
IN |
denotes |
on |
T6879 |
945-946 |
DT |
denotes |
a |
T6880 |
977-987 |
NN |
denotes |
microscope |
T6881 |
947-953 |
NNP |
denotes |
BioRad |
T6882 |
954-962 |
NNP |
denotes |
Radiance |
T6883 |
963-967 |
CD |
denotes |
2100 |
T6884 |
968-976 |
JJ |
denotes |
confocal |
T6885 |
988-989 |
-LRB- |
denotes |
( |
T6886 |
989-995 |
NNP |
denotes |
Biorad |
T6887 |
995-996 |
-RRB- |
denotes |
) |
T6888 |
996-998 |
, |
denotes |
, |
T6889 |
998-1002 |
IN |
denotes |
with |
T6890 |
1003-1012 |
JJ |
denotes |
identical |
T6891 |
1022-1030 |
NNS |
denotes |
settings |
T6892 |
1013-1021 |
NN |
denotes |
exposure |
T6893 |
1031-1034 |
IN |
denotes |
for |
T6894 |
1035-1039 |
JJ |
denotes |
wild |
T6895 |
1040-1044 |
NN |
denotes |
type |
T6896 |
1039-1040 |
HYPH |
denotes |
- |
T6897 |
1061-1067 |
NNS |
denotes |
images |
T6898 |
1045-1048 |
CC |
denotes |
and |
T6899 |
1049-1055 |
JJ |
denotes |
mutant |
T6900 |
1056-1060 |
JJ |
denotes |
like |
T6901 |
1067-1068 |
. |
denotes |
. |
T6902 |
1068-1203 |
sentence |
denotes |
All comparisons of wild-type and mutant staining were performed in Adobe Photoshop with identical brightness and contrast adjustments. |
T6903 |
1069-1072 |
DT |
denotes |
All |
T6904 |
1073-1084 |
NNS |
denotes |
comparisons |
T6905 |
1123-1132 |
VBN |
denotes |
performed |
T6906 |
1085-1087 |
IN |
denotes |
of |
T6907 |
1088-1092 |
JJ |
denotes |
wild |
T6908 |
1093-1097 |
NN |
denotes |
type |
T6909 |
1092-1093 |
HYPH |
denotes |
- |
T6910 |
1109-1117 |
NN |
denotes |
staining |
T6911 |
1098-1101 |
CC |
denotes |
and |
T6912 |
1102-1108 |
NN |
denotes |
mutant |
T6913 |
1118-1122 |
VBD |
denotes |
were |
T6914 |
1133-1135 |
IN |
denotes |
in |
T6915 |
1136-1141 |
NNP |
denotes |
Adobe |
T6916 |
1142-1151 |
NNP |
denotes |
Photoshop |
T6917 |
1152-1156 |
IN |
denotes |
with |
T6918 |
1157-1166 |
JJ |
denotes |
identical |
T6919 |
1191-1202 |
NNS |
denotes |
adjustments |
T6920 |
1167-1177 |
NN |
denotes |
brightness |
T6921 |
1178-1181 |
CC |
denotes |
and |
T6922 |
1182-1190 |
NN |
denotes |
contrast |
T6923 |
1202-1203 |
. |
denotes |
. |
R4100 |
T6713 |
T6712 |
nummod |
7.4,pH |
R4101 |
T6714 |
T6706 |
punct |
", ",formaldehyde |
R4102 |
T6715 |
T6706 |
prep |
for,formaldehyde |
R4103 |
T6716 |
T6717 |
nummod |
20,minutes |
R4104 |
T6717 |
T6715 |
pobj |
minutes,for |
R4105 |
T6718 |
T6702 |
punct |
", ",with |
R4106 |
T6719 |
T6702 |
cc |
or,with |
R4108 |
T6721 |
T6722 |
npadvmod |
ice,cold |
R4109 |
T6722 |
T6724 |
amod |
cold,acetone |
R4110 |
T6723 |
T6722 |
punct |
-,cold |
R4111 |
T6724 |
T6720 |
pobj |
acetone,with |
R4112 |
T6725 |
T6724 |
compound |
methanol,acetone |
R4113 |
T6726 |
T6724 |
punct |
/,acetone |
R4114 |
T6727 |
T6728 |
punct |
(,1 |
R4115 |
T6728 |
T6724 |
parataxis |
1,acetone |
R4116 |
T6729 |
T6730 |
punct |
:,1 |
R4117 |
T6730 |
T6728 |
prep |
1,1 |
R4252 |
T6871 |
T6870 |
compound |
mounting,medium |
R4253 |
T6872 |
T6873 |
punct |
(,Laboratories |
R4254 |
T6873 |
T6870 |
parataxis |
Laboratories,medium |
R4255 |
T6874 |
T6873 |
compound |
Vector,Laboratories |
R4256 |
T6875 |
T6873 |
punct |
),Laboratories |
R4257 |
T6876 |
T6862 |
cc |
and,coverslipped |
R4258 |
T6877 |
T6862 |
conj |
analyzed,coverslipped |
R4259 |
T6878 |
T6877 |
prep |
on,analyzed |
R4260 |
T6879 |
T6880 |
det |
a,microscope |
R4261 |
T6880 |
T6878 |
pobj |
microscope,on |
R4262 |
T6881 |
T6882 |
nmod |
BioRad,Radiance |
R4263 |
T6882 |
T6880 |
nmod |
Radiance,microscope |
R4264 |
T6883 |
T6882 |
nummod |
2100,Radiance |
R4053 |
T6663 |
T6664 |
prep |
For,seeded |
R4054 |
T6665 |
T6663 |
pobj |
immunostaining,For |
R4055 |
T6666 |
T6664 |
punct |
", ",seeded |
R4056 |
T6667 |
T6664 |
nsubj |
cells,seeded |
R4057 |
T6668 |
T6664 |
aux |
were,seeded |
R4058 |
T6669 |
T6664 |
prep |
at,seeded |
R4059 |
T6670 |
T6671 |
det |
a,density |
R4060 |
T6671 |
T6669 |
pobj |
density,at |
R4061 |
T6672 |
T6671 |
prep |
of,density |
R4062 |
T6673 |
T6674 |
quantmod |
3,5 |
R4063 |
T6674 |
T6676 |
quantmod |
5,104 |
R4064 |
T6675 |
T6674 |
punct |
–,5 |
R4065 |
T6676 |
T6678 |
nummod |
104,cells |
R4066 |
T6677 |
T6676 |
punct |
×,104 |
R4067 |
T6678 |
T6672 |
pobj |
cells,of |
R4068 |
T6679 |
T6678 |
prep |
per,cells |
R4069 |
T6680 |
T6679 |
pobj |
well,per |
R4070 |
T6681 |
T6664 |
prep |
in,seeded |
R4071 |
T6682 |
T6683 |
nummod |
4,well |
R4072 |
T6683 |
T6685 |
compound |
well,slides |
R4073 |
T6684 |
T6683 |
punct |
-,well |
R4074 |
T6685 |
T6681 |
pobj |
slides,in |
R4075 |
T6686 |
T6685 |
compound |
chamber,slides |
R4076 |
T6687 |
T6685 |
punct |
-,slides |
R4077 |
T6688 |
T6664 |
cc |
and,seeded |
R4078 |
T6689 |
T6664 |
conj |
grown,seeded |
R4079 |
T6690 |
T6689 |
advmod |
overnight,grown |
R4080 |
T6691 |
T6689 |
prep |
at,grown |
R4081 |
T6692 |
T6693 |
nummod |
33,°C |
R4082 |
T6693 |
T6691 |
pobj |
°C,at |
R4083 |
T6694 |
T6664 |
punct |
", ",seeded |
R4084 |
T6695 |
T6696 |
mark |
unless,indicated |
R4085 |
T6696 |
T6664 |
advcl |
indicated,seeded |
R4086 |
T6697 |
T6696 |
advmod |
otherwise,indicated |
R4087 |
T6698 |
T6664 |
punct |
.,seeded |
R4088 |
T6700 |
T6701 |
nsubj |
Fixation,was |
R4089 |
T6702 |
T6701 |
prep |
with,was |
R4090 |
T6703 |
T6704 |
npadvmod |
ice,cold |
R4091 |
T6704 |
T6706 |
amod |
cold,formaldehyde |
R4092 |
T6705 |
T6704 |
punct |
-,cold |
R4093 |
T6706 |
T6702 |
pobj |
formaldehyde,with |
R4094 |
T6707 |
T6708 |
nummod |
4,% |
R4095 |
T6708 |
T6706 |
compound |
%,formaldehyde |
R4096 |
T6709 |
T6706 |
prep |
in,formaldehyde |
R4097 |
T6710 |
T6709 |
pobj |
PBS,in |
R4098 |
T6711 |
T6710 |
punct |
", ",PBS |
R4099 |
T6712 |
T6710 |
npadvmod |
pH,PBS |
R4107 |
T6720 |
T6702 |
conj |
with,with |
R4118 |
T6731 |
T6728 |
punct |
),1 |
R4119 |
T6732 |
T6724 |
prep |
for,acetone |
R4120 |
T6733 |
T6734 |
nummod |
10,minutes |
R4121 |
T6734 |
T6732 |
pobj |
minutes,for |
R4122 |
T6735 |
T6724 |
prep |
at,acetone |
R4123 |
T6736 |
T6737 |
punct |
-,20 |
R4124 |
T6737 |
T6738 |
nummod |
20,°C |
R4125 |
T6738 |
T6735 |
pobj |
°C,at |
R4126 |
T6739 |
T6724 |
acl |
followed,acetone |
R4127 |
T6740 |
T6739 |
agent |
by,followed |
R4128 |
T6741 |
T6742 |
compound |
air,drying |
R4129 |
T6742 |
T6740 |
pobj |
drying,by |
R4130 |
T6743 |
T6742 |
punct |
-,drying |
R4131 |
T6744 |
T6745 |
punct |
(,dependent |
R4132 |
T6745 |
T6739 |
parataxis |
dependent,followed |
R4133 |
T6746 |
T6745 |
npadvmod |
antibody,dependent |
R4134 |
T6747 |
T6745 |
punct |
-,dependent |
R4135 |
T6748 |
T6745 |
punct |
),dependent |
R4136 |
T6749 |
T6701 |
punct |
.,was |
R4137 |
T6751 |
T6752 |
nsubjpass |
Cells,washed |
R4138 |
T6753 |
T6752 |
auxpass |
were,washed |
R4139 |
T6754 |
T6752 |
prep |
with,washed |
R4140 |
T6755 |
T6754 |
pobj |
PBS,with |
R4141 |
T6756 |
T6757 |
advmod |
at,2 |
R4142 |
T6757 |
T6752 |
npadvmod |
2,washed |
R4143 |
T6758 |
T6757 |
advmod |
least,2 |
R4144 |
T6759 |
T6757 |
quantmod |
times,2 |
R4145 |
T6760 |
T6752 |
punct |
", ",washed |
R4146 |
T6761 |
T6762 |
nummod |
5,minutes |
R4147 |
T6762 |
T6752 |
npadvmod |
minutes,washed |
R4148 |
T6763 |
T6762 |
prep |
per,minutes |
R4149 |
T6764 |
T6763 |
pobj |
wash,per |
R4150 |
T6765 |
T6752 |
punct |
", ",washed |
R4151 |
T6766 |
T6752 |
prep |
between,washed |
R4152 |
T6767 |
T6766 |
pobj |
each,between |
R4153 |
T6768 |
T6767 |
prep |
of,each |
R4154 |
T6769 |
T6770 |
det |
the,steps |
R4155 |
T6770 |
T6768 |
pobj |
steps,of |
R4156 |
T6771 |
T6770 |
amod |
following,steps |
R4157 |
T6772 |
T6770 |
prep |
of,steps |
R4158 |
T6773 |
T6774 |
det |
the,procedure |
R4159 |
T6774 |
T6772 |
pobj |
procedure,of |
R4160 |
T6775 |
T6774 |
compound |
staining,procedure |
R4161 |
T6776 |
T6770 |
punct |
: ,steps |
R4162 |
T6777 |
T6778 |
nummod |
0.1,M |
R4163 |
T6778 |
T6779 |
compound |
M,glycine |
R4164 |
T6779 |
T6770 |
appos |
glycine,steps |
R4165 |
T6780 |
T6779 |
prep |
in,glycine |
R4166 |
T6781 |
T6780 |
pobj |
PBS,in |
R4167 |
T6782 |
T6779 |
prep |
for,glycine |
R4168 |
T6783 |
T6784 |
nummod |
5,minutes |
R4169 |
T6784 |
T6782 |
pobj |
minutes,for |
R4170 |
T6785 |
T6779 |
punct |
", ",glycine |
R4171 |
T6786 |
T6787 |
nummod |
0.05,% |
R4172 |
T6787 |
T6788 |
nmod |
%,X |
R4173 |
T6788 |
T6779 |
appos |
X,glycine |
R4174 |
T6789 |
T6787 |
cc |
or,% |
R4175 |
T6790 |
T6791 |
nummod |
0.1,% |
R4176 |
T6791 |
T6787 |
conj |
%,% |
R4177 |
T6792 |
T6788 |
punct |
(,X |
R4178 |
T6793 |
T6794 |
npadvmod |
antibody,dependent |
R4179 |
T6794 |
T6788 |
amod |
dependent,X |
R4180 |
T6795 |
T6794 |
punct |
-,dependent |
R4181 |
T6796 |
T6788 |
punct |
),X |
R4182 |
T6797 |
T6788 |
compound |
Triton,X |
R4183 |
T6798 |
T6788 |
punct |
-,X |
R4184 |
T6799 |
T6788 |
nummod |
100,X |
R4185 |
T6800 |
T6801 |
punct |
(,Scientific |
R4186 |
T6801 |
T6788 |
parataxis |
Scientific,X |
R4187 |
T6802 |
T6801 |
compound |
Fisher,Scientific |
R4188 |
T6803 |
T6801 |
punct |
),Scientific |
R4189 |
T6804 |
T6788 |
prep |
in,X |
R4190 |
T6805 |
T6804 |
pobj |
PBS,in |
R4191 |
T6806 |
T6788 |
prep |
for,X |
R4192 |
T6807 |
T6808 |
nummod |
5,minutes |
R4193 |
T6808 |
T6806 |
pobj |
minutes,for |
R4194 |
T6809 |
T6779 |
punct |
", ",glycine |
R4195 |
T6810 |
T6811 |
nummod |
2,% |
R4196 |
T6811 |
T6779 |
appos |
%,glycine |
R4197 |
T6812 |
T6813 |
amod |
bovine,albumin |
R4198 |
T6813 |
T6811 |
appos |
albumin,% |
R4199 |
T6814 |
T6813 |
compound |
serum,albumin |
R4200 |
T6815 |
T6813 |
punct |
(,albumin |
R4201 |
T6816 |
T6813 |
appos |
BSA,albumin |
R4202 |
T6817 |
T6811 |
punct |
),% |
R4203 |
T6818 |
T6811 |
prep |
in,% |
R4204 |
T6819 |
T6818 |
pobj |
PBS,in |
R4205 |
T6820 |
T6811 |
prep |
for,% |
R4206 |
T6821 |
T6822 |
nummod |
30,minutes |
R4207 |
T6822 |
T6820 |
pobj |
minutes,for |
R4208 |
T6823 |
T6779 |
punct |
", ",glycine |
R4209 |
T6824 |
T6825 |
amod |
primary,antibody |
R4210 |
T6825 |
T6779 |
appos |
antibody,glycine |
R4211 |
T6826 |
T6825 |
acl |
diluted,antibody |
R4212 |
T6827 |
T6826 |
prep |
in,diluted |
R4213 |
T6828 |
T6829 |
nummod |
2,% |
R4214 |
T6829 |
T6830 |
compound |
%,PBS |
R4215 |
T6830 |
T6827 |
pobj |
PBS,in |
R4216 |
T6831 |
T6830 |
compound |
BSA,PBS |
R4217 |
T6832 |
T6830 |
punct |
/,PBS |
R4218 |
T6833 |
T6826 |
prep |
for,diluted |
R4219 |
T6834 |
T6835 |
nummod |
90,minutes |
R4220 |
T6835 |
T6833 |
pobj |
minutes,for |
R4221 |
T6836 |
T6779 |
punct |
", ",glycine |
R4222 |
T6837 |
T6838 |
amod |
secondary,antibody |
R4223 |
T6838 |
T6779 |
appos |
antibody,glycine |
R4224 |
T6839 |
T6838 |
acl |
diluted,antibody |
R4225 |
T6840 |
T6839 |
prep |
in,diluted |
R4226 |
T6841 |
T6842 |
nummod |
2,% |
R4227 |
T6842 |
T6843 |
compound |
%,PBS |
R4228 |
T6843 |
T6840 |
pobj |
PBS,in |
R4229 |
T6844 |
T6843 |
compound |
BSA,PBS |
R4230 |
T6845 |
T6843 |
punct |
/,PBS |
R4231 |
T6846 |
T6839 |
prep |
for,diluted |
R4232 |
T6847 |
T6848 |
nummod |
60,minutes |
R4233 |
T6848 |
T6846 |
pobj |
minutes,for |
R4234 |
T6849 |
T6752 |
punct |
.,washed |
R4235 |
T6851 |
T6852 |
det |
All,incubations |
R4236 |
T6852 |
T6853 |
nsubjpass |
incubations,carried |
R4237 |
T6854 |
T6853 |
auxpass |
were,carried |
R4238 |
T6855 |
T6853 |
prt |
out,carried |
R4239 |
T6856 |
T6853 |
prep |
at,carried |
R4240 |
T6857 |
T6858 |
compound |
room,temperature |
R4241 |
T6858 |
T6856 |
pobj |
temperature,at |
R4242 |
T6859 |
T6853 |
punct |
.,carried |
R4243 |
T6861 |
T6862 |
prep |
Following,coverslipped |
R4244 |
T6863 |
T6864 |
compound |
staining,procedures |
R4245 |
T6864 |
T6861 |
pobj |
procedures,Following |
R4246 |
T6865 |
T6862 |
punct |
", ",coverslipped |
R4247 |
T6866 |
T6862 |
nsubjpass |
slides,coverslipped |
R4248 |
T6867 |
T6862 |
auxpass |
were,coverslipped |
R4249 |
T6868 |
T6862 |
prep |
with,coverslipped |
R4250 |
T6869 |
T6870 |
compound |
Vectashield,medium |
R4251 |
T6870 |
T6868 |
pobj |
medium,with |
R4265 |
T6884 |
T6880 |
amod |
confocal,microscope |
R4266 |
T6885 |
T6886 |
punct |
(,Biorad |
R4267 |
T6886 |
T6880 |
parataxis |
Biorad,microscope |
R4268 |
T6887 |
T6886 |
punct |
),Biorad |
R4269 |
T6888 |
T6877 |
punct |
", ",analyzed |
R4270 |
T6889 |
T6877 |
prep |
with,analyzed |
R4271 |
T6890 |
T6891 |
amod |
identical,settings |
R4272 |
T6891 |
T6889 |
pobj |
settings,with |
R4273 |
T6892 |
T6891 |
compound |
exposure,settings |
R4274 |
T6893 |
T6891 |
prep |
for,settings |
R4275 |
T6894 |
T6895 |
amod |
wild,type |
R4276 |
T6895 |
T6897 |
nmod |
type,images |
R4277 |
T6896 |
T6895 |
punct |
-,type |
R4278 |
T6897 |
T6893 |
pobj |
images,for |
R4279 |
T6898 |
T6895 |
cc |
and,type |
R4280 |
T6899 |
T6895 |
conj |
mutant,type |
R4281 |
T6900 |
T6897 |
amod |
like,images |
R4282 |
T6901 |
T6862 |
punct |
.,coverslipped |
R4283 |
T6903 |
T6904 |
det |
All,comparisons |
R4284 |
T6904 |
T6905 |
nsubjpass |
comparisons,performed |
R4285 |
T6906 |
T6904 |
prep |
of,comparisons |
R4286 |
T6907 |
T6908 |
amod |
wild,type |
R4287 |
T6908 |
T6910 |
nmod |
type,staining |
R4288 |
T6909 |
T6908 |
punct |
-,type |
R4289 |
T6910 |
T6906 |
pobj |
staining,of |
R4290 |
T6911 |
T6908 |
cc |
and,type |
R4291 |
T6912 |
T6908 |
conj |
mutant,type |
R4292 |
T6913 |
T6905 |
auxpass |
were,performed |
R4293 |
T6914 |
T6905 |
prep |
in,performed |
R4294 |
T6915 |
T6916 |
compound |
Adobe,Photoshop |
R4295 |
T6916 |
T6914 |
pobj |
Photoshop,in |
R4296 |
T6917 |
T6905 |
prep |
with,performed |
R4297 |
T6918 |
T6919 |
amod |
identical,adjustments |
R4298 |
T6919 |
T6917 |
pobj |
adjustments,with |
R4299 |
T6920 |
T6919 |
nmod |
brightness,adjustments |
R4300 |
T6921 |
T6920 |
cc |
and,brightness |
R4301 |
T6922 |
T6920 |
conj |
contrast,brightness |
R4302 |
T6923 |
T6905 |
punct |
.,performed |