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PMC:539297 / 30859-32743 JSONTXT

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craft-ca-core-dev

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Id Subject Object Predicate Lexical cue
T6601 147-152 GO:0040007 denotes grown
T6602 230-242 CHEBI:16842 denotes formaldehyde
T6603 292-300 CHEBI:17790 denotes methanol
T6604 301-308 CHEBI:15347 denotes acetone
T6605 363-371 GO:0042571 denotes antibody
T6606 563-571 GO:0042571 denotes antibody
T6607 583-595 CHEBI:9750 denotes Triton X-100
T6608 641-647 NCBITaxon:27592 denotes bovine
T6609 648-653 UBERON:0001977 denotes serum
T6610 648-661 PR:000003918 denotes serum albumin
T6611 699-707 GO:0042571 denotes antibody
T6612 756-764 GO:0042571 denotes antibody
T6613 1324-1328 CHEBI:9754 denotes Tris
T6614 1341-1353 CHEBI:9750 denotes Triton X-100
T6615 1355-1363 GO:0016020 denotes membrane
T6616 1446-1457 CHEBI:7989 denotes pepstatin A
T6617 1486-1495 CHEBI:6426 denotes leupeptin
T6618 1740-1743 CHEBI:8984 denotes SDS
T6619 1806-1810 CHEBI:9754 denotes tris
T6620 1811-1818 CHEBI:46760 denotes tricine
T6621 1819-1822 CHEBI:8984 denotes SDS
T6622 1847-1856 PR:000022190 denotes subunit c

craft-sa-dev

Id Subject Object Predicate Lexical cue
T6658 0-14 NN denotes Immunostaining
T6659 30-38 NN denotes analysis
T6660 15-18 CC denotes and
T6661 19-29 NN denotes Immunoblot
T6662 38-199 sentence denotes For immunostaining, cells were seeded at a density of 3–5 × 104 cells per well in 4-well chamber-slides and grown overnight at 33°C, unless indicated otherwise.
T6663 39-42 IN denotes For
T6664 70-76 VBN denotes seeded
T6665 43-57 NN denotes immunostaining
T6666 57-59 , denotes ,
T6667 59-64 NNS denotes cells
T6668 65-69 VBD denotes were
T6669 77-79 IN denotes at
T6670 80-81 DT denotes a
T6671 82-89 NN denotes density
T6672 90-92 IN denotes of
T6673 93-94 CD denotes 3
T6674 95-96 CD denotes 5
T6675 94-95 SYM denotes –
T6676 99-102 CD denotes 104
T6677 97-98 SYM denotes ×
T6678 103-108 NNS denotes cells
T6679 109-112 IN denotes per
T6680 113-117 NN denotes well
T6681 118-120 IN denotes in
T6682 121-122 CD denotes 4
T6683 123-127 NN denotes well
T6684 122-123 HYPH denotes -
T6685 136-142 NNS denotes slides
T6686 128-135 NN denotes chamber
T6687 135-136 HYPH denotes -
T6688 143-146 CC denotes and
T6689 147-152 VBN denotes grown
T6690 153-162 RB denotes overnight
T6691 163-165 IN denotes at
T6692 166-168 CD denotes 33
T6693 168-170 NN denotes °C
T6694 170-172 , denotes ,
T6695 172-178 IN denotes unless
T6696 179-188 VBN denotes indicated
T6697 189-198 RB denotes otherwise
T6698 198-199 . denotes .
T6699 199-383 sentence denotes Fixation was with ice-cold 4% formaldehyde in PBS, pH 7.4, for 20 minutes, or with ice-cold methanol/acetone (1:1) for 10 minutes at -20°C followed by air-drying (antibody-dependent).
T6700 200-208 NN denotes Fixation
T6701 209-212 VBD denotes was
T6702 213-217 IN denotes with
T6703 218-221 NN denotes ice
T6704 222-226 JJ denotes cold
T6705 221-222 HYPH denotes -
T6706 230-242 NN denotes formaldehyde
T6707 227-228 CD denotes 4
T6708 228-229 NN denotes %
T6709 243-245 IN denotes in
T6710 246-249 NN denotes PBS
T6711 249-251 , denotes ,
T6712 251-253 NN denotes pH
T6713 254-257 CD denotes 7.4
T6714 257-259 , denotes ,
T6715 259-262 IN denotes for
T6716 263-265 CD denotes 20
T6717 266-273 NNS denotes minutes
T6718 273-275 , denotes ,
T6719 275-277 CC denotes or
T6720 278-282 IN denotes with
T6721 283-286 NN denotes ice
T6722 287-291 JJ denotes cold
T6723 286-287 HYPH denotes -
T6724 301-308 NN denotes acetone
T6725 292-300 NN denotes methanol
T6726 300-301 HYPH denotes /
T6727 309-310 -LRB- denotes (
T6728 310-311 CD denotes 1
T6729 311-312 SYM denotes :
T6730 312-313 CD denotes 1
T6731 313-314 -RRB- denotes )
T6732 315-318 IN denotes for
T6733 319-321 CD denotes 10
T6734 322-329 NNS denotes minutes
T6735 330-332 IN denotes at
T6736 333-334 SYM denotes -
T6737 334-336 CD denotes 20
T6738 336-338 NN denotes °C
T6739 339-347 VBN denotes followed
T6740 348-350 IN denotes by
T6741 351-354 NN denotes air
T6742 355-361 NN denotes drying
T6743 354-355 HYPH denotes -
T6744 362-363 -LRB- denotes (
T6745 372-381 JJ denotes dependent
T6746 363-371 NN denotes antibody
T6747 371-372 HYPH denotes -
T6748 381-382 -RRB- denotes )
T6749 382-383 . denotes .
T6750 383-802 sentence denotes Cells were washed with PBS at least 2 times, 5 minutes per wash, between each of the following steps of the staining procedure: 0.1 M glycine in PBS for 5 minutes, 0.05% or 0.1% (antibody-dependent) Triton X-100 (Fisher Scientific) in PBS for 5 minutes, 2% bovine serum albumin (BSA) in PBS for 30 minutes, primary antibody diluted in 2% BSA/PBS for 90 minutes, secondary antibody diluted in 2% BSA/PBS for 60 minutes.
T6751 384-389 NNS denotes Cells
T6752 395-401 VBN denotes washed
T6753 390-394 VBD denotes were
T6754 402-406 IN denotes with
T6755 407-410 NN denotes PBS
T6756 411-413 RB denotes at
T6757 420-421 CD denotes 2
T6758 414-419 RBS denotes least
T6759 422-427 NNS denotes times
T6760 427-429 , denotes ,
T6761 429-430 CD denotes 5
T6762 431-438 NNS denotes minutes
T6763 439-442 IN denotes per
T6764 443-447 NN denotes wash
T6765 447-449 , denotes ,
T6766 449-456 IN denotes between
T6767 457-461 DT denotes each
T6768 462-464 IN denotes of
T6769 465-468 DT denotes the
T6770 479-484 NNS denotes steps
T6771 469-478 JJ denotes following
T6772 485-487 IN denotes of
T6773 488-491 DT denotes the
T6774 501-510 NN denotes procedure
T6775 492-500 NN denotes staining
T6776 510-512 : denotes :
T6777 512-515 CD denotes 0.1
T6778 516-517 NN denotes M
T6779 518-525 NN denotes glycine
T6780 526-528 IN denotes in
T6781 529-532 NN denotes PBS
T6782 533-536 IN denotes for
T6783 537-538 CD denotes 5
T6784 539-546 NNS denotes minutes
T6785 546-548 , denotes ,
T6786 548-552 CD denotes 0.05
T6787 552-553 NN denotes %
T6788 590-591 NN denotes X
T6789 554-556 CC denotes or
T6790 557-560 CD denotes 0.1
T6791 560-561 NN denotes %
T6792 562-563 -LRB- denotes (
T6793 563-571 NN denotes antibody
T6794 572-581 JJ denotes dependent
T6795 571-572 HYPH denotes -
T6796 581-582 -RRB- denotes )
T6797 583-589 NN denotes Triton
T6798 591-592 HYPH denotes -
T6799 592-595 CD denotes 100
T6800 596-597 -LRB- denotes (
T6801 604-614 NNP denotes Scientific
T6802 597-603 NNP denotes Fisher
T6803 614-615 -RRB- denotes )
T6804 616-618 IN denotes in
T6805 619-622 NN denotes PBS
T6806 623-626 IN denotes for
T6807 627-628 CD denotes 5
T6808 629-636 NNS denotes minutes
T6809 636-638 , denotes ,
T6810 638-639 CD denotes 2
T6811 639-640 NN denotes %
T6812 641-647 JJ denotes bovine
T6813 654-661 NN denotes albumin
T6814 648-653 NN denotes serum
T6815 662-663 -LRB- denotes (
T6816 663-666 NN denotes BSA
T6817 666-667 -RRB- denotes )
T6818 668-670 IN denotes in
T6819 671-674 NN denotes PBS
T6820 675-678 IN denotes for
T6821 679-681 CD denotes 30
T6822 682-689 NNS denotes minutes
T6823 689-691 , denotes ,
T6824 691-698 JJ denotes primary
T6825 699-707 NN denotes antibody
T6826 708-715 VBN denotes diluted
T6827 716-718 IN denotes in
T6828 719-720 CD denotes 2
T6829 720-721 NN denotes %
T6830 726-729 NN denotes PBS
T6831 722-725 NN denotes BSA
T6832 725-726 HYPH denotes /
T6833 730-733 IN denotes for
T6834 734-736 CD denotes 90
T6835 737-744 NNS denotes minutes
T6836 744-746 , denotes ,
T6837 746-755 JJ denotes secondary
T6838 756-764 NN denotes antibody
T6839 765-772 VBN denotes diluted
T6840 773-775 IN denotes in
T6841 776-777 CD denotes 2
T6842 777-778 NN denotes %
T6843 783-786 NN denotes PBS
T6844 779-782 NN denotes BSA
T6845 782-783 HYPH denotes /
T6846 787-790 IN denotes for
T6847 791-793 CD denotes 60
T6848 794-801 NNS denotes minutes
T6849 801-802 . denotes .
T6850 802-856 sentence denotes All incubations were carried out at room temperature.
T6851 803-806 DT denotes All
T6852 807-818 NNS denotes incubations
T6853 824-831 VBN denotes carried
T6854 819-823 VBD denotes were
T6855 832-835 RP denotes out
T6856 836-838 IN denotes at
T6857 839-843 NN denotes room
T6858 844-855 NN denotes temperature
T6859 855-856 . denotes .
T6860 856-1107 sentence denotes Following staining procedures, slides were coverslipped with Vectashield mounting medium (Vector Laboratories) and analyzed on a BioRad Radiance 2100 confocal microscope (Biorad), with identical exposure settings for wild-type and mutant like images.
T6861 857-866 VBG denotes Following
T6862 900-912 VBN denotes coverslipped
T6863 867-875 NN denotes staining
T6864 876-886 NNS denotes procedures
T6865 886-888 , denotes ,
T6866 888-894 NNS denotes slides
T6867 895-899 VBD denotes were
T6868 913-917 IN denotes with
T6869 918-929 NNP denotes Vectashield
T6870 939-945 NN denotes medium
T6871 930-938 NN denotes mounting
T6872 946-947 -LRB- denotes (
T6873 954-966 NNP denotes Laboratories
T6874 947-953 NNP denotes Vector
T6875 966-967 -RRB- denotes )
T6876 968-971 CC denotes and
T6877 972-980 VBN denotes analyzed
T6878 981-983 IN denotes on
T6879 984-985 DT denotes a
T6880 1016-1026 NN denotes microscope
T6881 986-992 NNP denotes BioRad
T6882 993-1001 NNP denotes Radiance
T6883 1002-1006 CD denotes 2100
T6884 1007-1015 JJ denotes confocal
T6885 1027-1028 -LRB- denotes (
T6886 1028-1034 NNP denotes Biorad
T6887 1034-1035 -RRB- denotes )
T6888 1035-1037 , denotes ,
T6889 1037-1041 IN denotes with
T6890 1042-1051 JJ denotes identical
T6891 1061-1069 NNS denotes settings
T6892 1052-1060 NN denotes exposure
T6893 1070-1073 IN denotes for
T6894 1074-1078 JJ denotes wild
T6895 1079-1083 NN denotes type
T6896 1078-1079 HYPH denotes -
T6897 1100-1106 NNS denotes images
T6898 1084-1087 CC denotes and
T6899 1088-1094 JJ denotes mutant
T6900 1095-1099 JJ denotes like
T6901 1106-1107 . denotes .
T6902 1107-1242 sentence denotes All comparisons of wild-type and mutant staining were performed in Adobe Photoshop with identical brightness and contrast adjustments.
T6903 1108-1111 DT denotes All
T6904 1112-1123 NNS denotes comparisons
T6905 1162-1171 VBN denotes performed
T6906 1124-1126 IN denotes of
T6907 1127-1131 JJ denotes wild
T6908 1132-1136 NN denotes type
T6909 1131-1132 HYPH denotes -
T6910 1148-1156 NN denotes staining
T6911 1137-1140 CC denotes and
T6912 1141-1147 NN denotes mutant
T6913 1157-1161 VBD denotes were
T6914 1172-1174 IN denotes in
T6915 1175-1180 NNP denotes Adobe
T6916 1181-1190 NNP denotes Photoshop
T6917 1191-1195 IN denotes with
T6918 1196-1205 JJ denotes identical
T6919 1230-1241 NNS denotes adjustments
T6920 1206-1216 NN denotes brightness
T6921 1217-1220 CC denotes and
T6922 1221-1229 NN denotes contrast
T6923 1241-1242 . denotes .
T6924 1242-1505 sentence denotes Total proteins were isolated from cell pellets by extraction with ice-cold 20 mM Tris, pH 7.4, 1% Triton X-100 (membrane-research grade, Roche), plus protease inhibitors (Complete mini tablet, 0.7 μg/ml pepstatin A, 2 μg/ml aprotinin, 5 μg/ml leupeptin [Roche]).
T6925 1243-1248 JJ denotes Total
T6926 1249-1257 NN denotes proteins
T6927 1263-1271 VBN denotes isolated
T6928 1258-1262 VBD denotes were
T6929 1272-1276 IN denotes from
T6930 1277-1281 NN denotes cell
T6931 1282-1289 NNS denotes pellets
T6932 1290-1292 IN denotes by
T6933 1293-1303 NN denotes extraction
T6934 1304-1308 IN denotes with
T6935 1309-1312 NN denotes ice
T6936 1324-1328 NN denotes Tris
T6937 1312-1313 HYPH denotes -
T6938 1313-1317 JJ denotes cold
T6939 1318-1320 CD denotes 20
T6940 1321-1323 NN denotes mM
T6941 1328-1330 , denotes ,
T6942 1330-1332 NN denotes pH
T6943 1333-1336 CD denotes 7.4
T6944 1336-1338 , denotes ,
T6945 1338-1339 CD denotes 1
T6946 1339-1340 NN denotes %
T6947 1348-1349 NN denotes X
T6948 1341-1347 NN denotes Triton
T6949 1349-1350 HYPH denotes -
T6950 1350-1353 CD denotes 100
T6951 1354-1355 -LRB- denotes (
T6952 1380-1385 NNP denotes Roche
T6953 1355-1363 NN denotes membrane
T6954 1364-1372 NN denotes research
T6955 1363-1364 HYPH denotes -
T6956 1373-1378 NN denotes grade
T6957 1378-1380 , denotes ,
T6958 1385-1386 -RRB- denotes )
T6959 1386-1388 , denotes ,
T6960 1388-1392 CC denotes plus
T6961 1393-1401 NN denotes protease
T6962 1402-1412 NNS denotes inhibitors
T6963 1413-1414 -LRB- denotes (
T6964 1414-1422 JJ denotes Complete
T6965 1428-1434 NN denotes tablet
T6966 1423-1427 NN denotes mini
T6967 1434-1436 , denotes ,
T6968 1436-1439 CD denotes 0.7
T6969 1440-1442 NN denotes μg
T6970 1456-1457 NN denotes A
T6971 1442-1443 SYM denotes /
T6972 1443-1445 NN denotes ml
T6973 1446-1455 NN denotes pepstatin
T6974 1457-1459 , denotes ,
T6975 1459-1460 CD denotes 2
T6976 1461-1463 NN denotes μg
T6977 1467-1476 NN denotes aprotinin
T6978 1463-1464 SYM denotes /
T6979 1464-1466 NN denotes ml
T6980 1476-1478 , denotes ,
T6981 1478-1479 CD denotes 5
T6982 1480-1482 NN denotes μg
T6983 1486-1495 NN denotes leupeptin
T6984 1482-1483 SYM denotes /
T6985 1483-1485 NN denotes ml
T6986 1496-1497 -LRB- denotes [
T6987 1497-1502 NNP denotes Roche
T6988 1502-1503 -RRB- denotes ]
T6989 1503-1504 -RRB- denotes )
T6990 1504-1505 . denotes .
T6991 1505-1650 sentence denotes Following homogenization through a 25-gauge needle (~10 passes), extracts were centrifuged at 1000 × g for 10 minutes, at 4°C, to remove debris.
T6992 1506-1515 VBG denotes Following
T6993 1585-1596 VBN denotes centrifuged
T6994 1516-1530 NN denotes homogenization
T6995 1531-1538 IN denotes through
T6996 1539-1540 DT denotes a
T6997 1550-1556 NN denotes needle
T6998 1541-1543 CD denotes 25
T6999 1544-1549 NN denotes gauge
T7000 1543-1544 HYPH denotes -
T7001 1557-1558 -LRB- denotes (
T7002 1562-1568 NNS denotes passes
T7003 1558-1559 SYM denotes ~
T7004 1559-1561 CD denotes 10
T7005 1568-1569 -RRB- denotes )
T7006 1569-1571 , denotes ,
T7007 1571-1579 NNS denotes extracts
T7008 1580-1584 VBD denotes were
T7009 1597-1599 IN denotes at
T7010 1600-1604 CD denotes 1000
T7011 1607-1608 NN denotes g
T7012 1605-1606 SYM denotes ×
T7013 1609-1612 IN denotes for
T7014 1613-1615 CD denotes 10
T7015 1616-1623 NNS denotes minutes
T7016 1623-1625 , denotes ,
T7017 1625-1627 IN denotes at
T7018 1628-1629 CD denotes 4
T7019 1629-1631 NN denotes °C
T7020 1631-1633 , denotes ,
T7021 1633-1635 TO denotes to
T7022 1636-1642 VB denotes remove
T7023 1643-1649 NN denotes debris
T7024 1649-1650 . denotes .
T7025 1650-1799 sentence denotes Typically, 20–40 μg of protein (determined by Bio-rad Dc Protein Assay) was separated by SDS-PAGE, for subsequent immunoblotting, as described [12].
T7026 1651-1660 RB denotes Typically
T7027 1727-1736 VBN denotes separated
T7028 1660-1662 , denotes ,
T7029 1662-1664 CD denotes 20
T7030 1665-1667 CD denotes 40
T7031 1664-1665 SYM denotes –
T7032 1668-1670 NN denotes μg
T7033 1671-1673 IN denotes of
T7034 1674-1681 NN denotes protein
T7035 1682-1683 -LRB- denotes (
T7036 1683-1693 VBN denotes determined
T7037 1694-1696 IN denotes by
T7038 1697-1700 NNP denotes Bio
T7039 1701-1704 NNP denotes rad
T7040 1700-1701 HYPH denotes -
T7041 1716-1721 NN denotes Assay
T7042 1705-1707 NN denotes Dc
T7043 1708-1715 NN denotes Protein
T7044 1721-1722 -RRB- denotes )
T7045 1723-1726 VBD denotes was
T7046 1737-1739 IN denotes by
T7047 1740-1743 NN denotes SDS
T7048 1744-1748 NN denotes PAGE
T7049 1743-1744 HYPH denotes -
T7050 1748-1750 , denotes ,
T7051 1750-1753 IN denotes for
T7052 1754-1764 JJ denotes subsequent
T7053 1765-1779 NN denotes immunoblotting
T7054 1779-1781 , denotes ,
T7055 1781-1783 IN denotes as
T7056 1784-1793 VBN denotes described
T7057 1794-1795 -LRB- denotes [
T7058 1795-1797 CD denotes 12
T7059 1797-1798 -RRB- denotes ]
T7060 1798-1799 . denotes .
T7061 1799-1884 sentence denotes 16.5% tris-tricine SDS-PAGE gels were used for subunit c immunoblotting experiments.
T7062 1800-1804 CD denotes 16.5
T7063 1804-1805 NN denotes %
T7064 1828-1832 NNS denotes gels
T7065 1806-1810 NN denotes tris
T7066 1811-1818 NN denotes tricine
T7067 1810-1811 HYPH denotes -
T7068 1819-1822 NN denotes SDS
T7069 1823-1827 NN denotes PAGE
T7070 1822-1823 HYPH denotes -
T7071 1838-1842 VBN denotes used
T7072 1833-1837 VBD denotes were
T7073 1843-1846 IN denotes for
T7074 1847-1854 NN denotes subunit
T7075 1855-1856 NN denotes c
T7076 1872-1883 NNS denotes experiments
T7077 1857-1871 NN denotes immunoblotting
T7078 1883-1884 . denotes .
R4100 T6713 T6712 nummod 7.4,pH
R4101 T6714 T6706 punct ", ",formaldehyde
R4102 T6715 T6706 prep for,formaldehyde
R4103 T6716 T6717 nummod 20,minutes
R4104 T6717 T6715 pobj minutes,for
R4105 T6718 T6702 punct ", ",with
R4106 T6719 T6702 cc or,with
R4108 T6721 T6722 npadvmod ice,cold
R4109 T6722 T6724 amod cold,acetone
R4110 T6723 T6722 punct -,cold
R4111 T6724 T6720 pobj acetone,with
R4112 T6725 T6724 compound methanol,acetone
R4113 T6726 T6724 punct /,acetone
R4114 T6727 T6728 punct (,1
R4115 T6728 T6724 parataxis 1,acetone
R4116 T6729 T6730 punct :,1
R4117 T6730 T6728 prep 1,1
R4252 T6871 T6870 compound mounting,medium
R4253 T6872 T6873 punct (,Laboratories
R4254 T6873 T6870 parataxis Laboratories,medium
R4255 T6874 T6873 compound Vector,Laboratories
R4256 T6875 T6873 punct ),Laboratories
R4257 T6876 T6862 cc and,coverslipped
R4258 T6877 T6862 conj analyzed,coverslipped
R4259 T6878 T6877 prep on,analyzed
R4260 T6879 T6880 det a,microscope
R4261 T6880 T6878 pobj microscope,on
R4262 T6881 T6882 nmod BioRad,Radiance
R4263 T6882 T6880 nmod Radiance,microscope
R4264 T6883 T6882 nummod 2100,Radiance
R4050 T6658 T6659 nmod Immunostaining,analysis
R4051 T6660 T6658 cc and,Immunostaining
R4052 T6661 T6658 conj Immunoblot,Immunostaining
R4053 T6663 T6664 prep For,seeded
R4054 T6665 T6663 pobj immunostaining,For
R4055 T6666 T6664 punct ", ",seeded
R4056 T6667 T6664 nsubj cells,seeded
R4057 T6668 T6664 aux were,seeded
R4058 T6669 T6664 prep at,seeded
R4059 T6670 T6671 det a,density
R4060 T6671 T6669 pobj density,at
R4061 T6672 T6671 prep of,density
R4062 T6673 T6674 quantmod 3,5
R4063 T6674 T6676 quantmod 5,104
R4064 T6675 T6674 punct –,5
R4065 T6676 T6678 nummod 104,cells
R4066 T6677 T6676 punct ×,104
R4067 T6678 T6672 pobj cells,of
R4068 T6679 T6678 prep per,cells
R4069 T6680 T6679 pobj well,per
R4070 T6681 T6664 prep in,seeded
R4071 T6682 T6683 nummod 4,well
R4072 T6683 T6685 compound well,slides
R4073 T6684 T6683 punct -,well
R4074 T6685 T6681 pobj slides,in
R4075 T6686 T6685 compound chamber,slides
R4076 T6687 T6685 punct -,slides
R4077 T6688 T6664 cc and,seeded
R4078 T6689 T6664 conj grown,seeded
R4079 T6690 T6689 advmod overnight,grown
R4080 T6691 T6689 prep at,grown
R4081 T6692 T6693 nummod 33,°C
R4082 T6693 T6691 pobj °C,at
R4083 T6694 T6664 punct ", ",seeded
R4084 T6695 T6696 mark unless,indicated
R4085 T6696 T6664 advcl indicated,seeded
R4086 T6697 T6696 advmod otherwise,indicated
R4087 T6698 T6664 punct .,seeded
R4088 T6700 T6701 nsubj Fixation,was
R4089 T6702 T6701 prep with,was
R4090 T6703 T6704 npadvmod ice,cold
R4091 T6704 T6706 amod cold,formaldehyde
R4092 T6705 T6704 punct -,cold
R4093 T6706 T6702 pobj formaldehyde,with
R4094 T6707 T6708 nummod 4,%
R4095 T6708 T6706 compound %,formaldehyde
R4096 T6709 T6706 prep in,formaldehyde
R4097 T6710 T6709 pobj PBS,in
R4098 T6711 T6710 punct ", ",PBS
R4099 T6712 T6710 npadvmod pH,PBS
R4107 T6720 T6702 conj with,with
R4118 T6731 T6728 punct ),1
R4119 T6732 T6724 prep for,acetone
R4120 T6733 T6734 nummod 10,minutes
R4121 T6734 T6732 pobj minutes,for
R4122 T6735 T6724 prep at,acetone
R4123 T6736 T6737 punct -,20
R4124 T6737 T6738 nummod 20,°C
R4125 T6738 T6735 pobj °C,at
R4126 T6739 T6724 acl followed,acetone
R4127 T6740 T6739 agent by,followed
R4128 T6741 T6742 compound air,drying
R4129 T6742 T6740 pobj drying,by
R4130 T6743 T6742 punct -,drying
R4131 T6744 T6745 punct (,dependent
R4132 T6745 T6739 parataxis dependent,followed
R4133 T6746 T6745 npadvmod antibody,dependent
R4134 T6747 T6745 punct -,dependent
R4135 T6748 T6745 punct ),dependent
R4136 T6749 T6701 punct .,was
R4137 T6751 T6752 nsubjpass Cells,washed
R4138 T6753 T6752 auxpass were,washed
R4139 T6754 T6752 prep with,washed
R4140 T6755 T6754 pobj PBS,with
R4141 T6756 T6757 advmod at,2
R4142 T6757 T6752 npadvmod 2,washed
R4143 T6758 T6757 advmod least,2
R4144 T6759 T6757 quantmod times,2
R4145 T6760 T6752 punct ", ",washed
R4146 T6761 T6762 nummod 5,minutes
R4147 T6762 T6752 npadvmod minutes,washed
R4148 T6763 T6762 prep per,minutes
R4149 T6764 T6763 pobj wash,per
R4150 T6765 T6752 punct ", ",washed
R4151 T6766 T6752 prep between,washed
R4152 T6767 T6766 pobj each,between
R4153 T6768 T6767 prep of,each
R4154 T6769 T6770 det the,steps
R4155 T6770 T6768 pobj steps,of
R4156 T6771 T6770 amod following,steps
R4157 T6772 T6770 prep of,steps
R4158 T6773 T6774 det the,procedure
R4159 T6774 T6772 pobj procedure,of
R4160 T6775 T6774 compound staining,procedure
R4161 T6776 T6770 punct : ,steps
R4162 T6777 T6778 nummod 0.1,M
R4163 T6778 T6779 compound M,glycine
R4164 T6779 T6770 appos glycine,steps
R4165 T6780 T6779 prep in,glycine
R4166 T6781 T6780 pobj PBS,in
R4167 T6782 T6779 prep for,glycine
R4168 T6783 T6784 nummod 5,minutes
R4169 T6784 T6782 pobj minutes,for
R4170 T6785 T6779 punct ", ",glycine
R4171 T6786 T6787 nummod 0.05,%
R4172 T6787 T6788 nmod %,X
R4173 T6788 T6779 appos X,glycine
R4174 T6789 T6787 cc or,%
R4175 T6790 T6791 nummod 0.1,%
R4176 T6791 T6787 conj %,%
R4177 T6792 T6788 punct (,X
R4178 T6793 T6794 npadvmod antibody,dependent
R4179 T6794 T6788 amod dependent,X
R4180 T6795 T6794 punct -,dependent
R4181 T6796 T6788 punct ),X
R4182 T6797 T6788 compound Triton,X
R4183 T6798 T6788 punct -,X
R4184 T6799 T6788 nummod 100,X
R4185 T6800 T6801 punct (,Scientific
R4186 T6801 T6788 parataxis Scientific,X
R4187 T6802 T6801 compound Fisher,Scientific
R4188 T6803 T6801 punct ),Scientific
R4189 T6804 T6788 prep in,X
R4190 T6805 T6804 pobj PBS,in
R4191 T6806 T6788 prep for,X
R4192 T6807 T6808 nummod 5,minutes
R4193 T6808 T6806 pobj minutes,for
R4194 T6809 T6779 punct ", ",glycine
R4195 T6810 T6811 nummod 2,%
R4196 T6811 T6779 appos %,glycine
R4197 T6812 T6813 amod bovine,albumin
R4198 T6813 T6811 appos albumin,%
R4199 T6814 T6813 compound serum,albumin
R4200 T6815 T6813 punct (,albumin
R4201 T6816 T6813 appos BSA,albumin
R4202 T6817 T6811 punct ),%
R4203 T6818 T6811 prep in,%
R4204 T6819 T6818 pobj PBS,in
R4205 T6820 T6811 prep for,%
R4206 T6821 T6822 nummod 30,minutes
R4207 T6822 T6820 pobj minutes,for
R4208 T6823 T6779 punct ", ",glycine
R4209 T6824 T6825 amod primary,antibody
R4210 T6825 T6779 appos antibody,glycine
R4211 T6826 T6825 acl diluted,antibody
R4212 T6827 T6826 prep in,diluted
R4213 T6828 T6829 nummod 2,%
R4214 T6829 T6830 compound %,PBS
R4215 T6830 T6827 pobj PBS,in
R4216 T6831 T6830 compound BSA,PBS
R4217 T6832 T6830 punct /,PBS
R4218 T6833 T6826 prep for,diluted
R4219 T6834 T6835 nummod 90,minutes
R4220 T6835 T6833 pobj minutes,for
R4221 T6836 T6779 punct ", ",glycine
R4222 T6837 T6838 amod secondary,antibody
R4223 T6838 T6779 appos antibody,glycine
R4224 T6839 T6838 acl diluted,antibody
R4225 T6840 T6839 prep in,diluted
R4226 T6841 T6842 nummod 2,%
R4227 T6842 T6843 compound %,PBS
R4228 T6843 T6840 pobj PBS,in
R4229 T6844 T6843 compound BSA,PBS
R4230 T6845 T6843 punct /,PBS
R4231 T6846 T6839 prep for,diluted
R4232 T6847 T6848 nummod 60,minutes
R4233 T6848 T6846 pobj minutes,for
R4234 T6849 T6752 punct .,washed
R4235 T6851 T6852 det All,incubations
R4236 T6852 T6853 nsubjpass incubations,carried
R4237 T6854 T6853 auxpass were,carried
R4238 T6855 T6853 prt out,carried
R4239 T6856 T6853 prep at,carried
R4240 T6857 T6858 compound room,temperature
R4241 T6858 T6856 pobj temperature,at
R4242 T6859 T6853 punct .,carried
R4243 T6861 T6862 prep Following,coverslipped
R4244 T6863 T6864 compound staining,procedures
R4245 T6864 T6861 pobj procedures,Following
R4246 T6865 T6862 punct ", ",coverslipped
R4247 T6866 T6862 nsubjpass slides,coverslipped
R4248 T6867 T6862 auxpass were,coverslipped
R4249 T6868 T6862 prep with,coverslipped
R4250 T6869 T6870 compound Vectashield,medium
R4251 T6870 T6868 pobj medium,with
R4265 T6884 T6880 amod confocal,microscope
R4266 T6885 T6886 punct (,Biorad
R4267 T6886 T6880 parataxis Biorad,microscope
R4268 T6887 T6886 punct ),Biorad
R4269 T6888 T6877 punct ", ",analyzed
R4270 T6889 T6877 prep with,analyzed
R4271 T6890 T6891 amod identical,settings
R4272 T6891 T6889 pobj settings,with
R4273 T6892 T6891 compound exposure,settings
R4274 T6893 T6891 prep for,settings
R4275 T6894 T6895 amod wild,type
R4276 T6895 T6897 nmod type,images
R4277 T6896 T6895 punct -,type
R4278 T6897 T6893 pobj images,for
R4279 T6898 T6895 cc and,type
R4280 T6899 T6895 conj mutant,type
R4281 T6900 T6897 amod like,images
R4282 T6901 T6862 punct .,coverslipped
R4283 T6903 T6904 det All,comparisons
R4284 T6904 T6905 nsubjpass comparisons,performed
R4285 T6906 T6904 prep of,comparisons
R4286 T6907 T6908 amod wild,type
R4287 T6908 T6910 nmod type,staining
R4288 T6909 T6908 punct -,type
R4289 T6910 T6906 pobj staining,of
R4290 T6911 T6908 cc and,type
R4291 T6912 T6908 conj mutant,type
R4292 T6913 T6905 auxpass were,performed
R4293 T6914 T6905 prep in,performed
R4294 T6915 T6916 compound Adobe,Photoshop
R4295 T6916 T6914 pobj Photoshop,in
R4296 T6917 T6905 prep with,performed
R4297 T6918 T6919 amod identical,adjustments
R4298 T6919 T6917 pobj adjustments,with
R4299 T6920 T6919 nmod brightness,adjustments
R4300 T6921 T6920 cc and,brightness
R4301 T6922 T6920 conj contrast,brightness
R4302 T6923 T6905 punct .,performed
R4303 T6925 T6926 amod Total,proteins
R4304 T6926 T6927 nsubjpass proteins,isolated
R4305 T6928 T6927 auxpass were,isolated
R4306 T6929 T6927 prep from,isolated
R4307 T6930 T6931 compound cell,pellets
R4308 T6931 T6929 pobj pellets,from
R4309 T6932 T6927 prep by,isolated
R4310 T6933 T6932 pobj extraction,by
R4311 T6934 T6933 prep with,extraction
R4312 T6935 T6936 nmod ice,Tris
R4313 T6936 T6934 pobj Tris,with
R4314 T6937 T6935 punct -,ice
R4315 T6938 T6935 amod cold,ice
R4316 T6939 T6940 nummod 20,mM
R4317 T6940 T6936 compound mM,Tris
R4318 T6941 T6936 punct ", ",Tris
R4319 T6942 T6936 npadvmod pH,Tris
R4320 T6943 T6942 nummod 7.4,pH
R4321 T6944 T6936 punct ", ",Tris
R4322 T6945 T6946 nummod 1,%
R4323 T6946 T6947 compound %,X
R4324 T6947 T6936 conj X,Tris
R4325 T6948 T6947 compound Triton,X
R4326 T6949 T6947 punct -,X
R4327 T6950 T6947 nummod 100,X
R4328 T6951 T6952 punct (,Roche
R4329 T6952 T6947 parataxis Roche,X
R4330 T6953 T6954 compound membrane,research
R4331 T6954 T6956 compound research,grade
R4332 T6955 T6954 punct -,research
R4333 T6956 T6952 dep grade,Roche
R4334 T6957 T6952 punct ", ",Roche
R4335 T6958 T6952 punct ),Roche
R4336 T6959 T6947 punct ", ",X
R4337 T6960 T6947 cc plus,X
R4338 T6961 T6962 compound protease,inhibitors
R4339 T6962 T6947 conj inhibitors,X
R4340 T6963 T6962 punct (,inhibitors
R4341 T6964 T6965 amod Complete,tablet
R4342 T6965 T6962 appos tablet,inhibitors
R4343 T6966 T6965 compound mini,tablet
R4344 T6967 T6965 punct ", ",tablet
R4345 T6968 T6969 nummod 0.7,μg
R4346 T6969 T6970 nmod μg,A
R4347 T6970 T6965 appos A,tablet
R4348 T6971 T6972 punct /,ml
R4349 T6972 T6969 prep ml,μg
R4350 T6973 T6970 compound pepstatin,A
R4351 T6974 T6965 punct ", ",tablet
R4352 T6975 T6976 nummod 2,μg
R4353 T6976 T6977 nmod μg,aprotinin
R4354 T6977 T6965 appos aprotinin,tablet
R4355 T6978 T6979 punct /,ml
R4356 T6979 T6976 prep ml,μg
R4357 T6980 T6965 punct ", ",tablet
R4358 T6981 T6982 nummod 5,μg
R4359 T6982 T6983 nmod μg,leupeptin
R4360 T6983 T6965 appos leupeptin,tablet
R4361 T6984 T6985 punct /,ml
R4362 T6985 T6982 prep ml,μg
R4363 T6986 T6987 punct [,Roche
R4364 T6987 T6983 parataxis Roche,leupeptin
R4365 T6988 T6987 punct ],Roche
R4366 T6989 T6927 punct ),isolated
R4367 T6990 T6927 punct .,isolated
R4368 T6992 T6993 prep Following,centrifuged
R4369 T6994 T6992 pobj homogenization,Following
R4370 T6995 T6994 prep through,homogenization
R4371 T6996 T6997 det a,needle
R4372 T6997 T6995 pobj needle,through
R4373 T6998 T6999 nummod 25,gauge
R4374 T6999 T6997 compound gauge,needle
R4375 T7000 T6999 punct -,gauge
R4376 T7001 T7002 punct (,passes
R4377 T7002 T6994 parataxis passes,homogenization
R4378 T7003 T7004 punct ~,10
R4379 T7004 T7002 nummod 10,passes
R4380 T7005 T7002 punct ),passes
R4381 T7006 T6993 punct ", ",centrifuged
R4382 T7007 T6993 nsubjpass extracts,centrifuged
R4383 T7008 T6993 auxpass were,centrifuged
R4384 T7009 T6993 prep at,centrifuged
R4385 T7010 T7011 nummod 1000,g
R4386 T7011 T7009 pobj g,at
R4387 T7012 T7011 punct ×,g
R4388 T7013 T6993 prep for,centrifuged
R4389 T7014 T7015 nummod 10,minutes
R4390 T7015 T7013 pobj minutes,for
R4391 T7016 T6993 punct ", ",centrifuged
R4392 T7017 T6993 prep at,centrifuged
R4393 T7018 T7019 nummod 4,°C
R4394 T7019 T7017 pobj °C,at
R4395 T7020 T6993 punct ", ",centrifuged
R4396 T7021 T7022 aux to,remove
R4397 T7022 T6993 advcl remove,centrifuged
R4398 T7023 T7022 dobj debris,remove
R4399 T7024 T6993 punct .,centrifuged
R4400 T7026 T7027 advmod Typically,separated
R4401 T7028 T7027 punct ", ",separated
R4402 T7029 T7030 quantmod 20,40
R4403 T7030 T7032 nummod 40,μg
R4404 T7031 T7030 punct –,40
R4405 T7032 T7027 nsubjpass μg,separated
R4406 T7033 T7032 prep of,μg
R4407 T7034 T7033 pobj protein,of
R4408 T7035 T7034 punct (,protein
R4409 T7036 T7034 acl determined,protein
R4410 T7037 T7036 agent by,determined
R4411 T7038 T7039 compound Bio,rad
R4412 T7039 T7041 compound rad,Assay
R4413 T7040 T7039 punct -,rad
R4414 T7041 T7037 pobj Assay,by
R4415 T7042 T7041 compound Dc,Assay
R4416 T7043 T7041 compound Protein,Assay
R4417 T7044 T7027 punct ),separated
R4418 T7045 T7027 auxpass was,separated
R4419 T7046 T7027 prep by,separated
R4420 T7047 T7048 compound SDS,PAGE
R4421 T7048 T7046 pobj PAGE,by
R4422 T7049 T7048 punct -,PAGE
R4423 T7050 T7027 punct ", ",separated
R4424 T7051 T7027 prep for,separated
R4425 T7052 T7053 amod subsequent,immunoblotting
R4426 T7053 T7051 pobj immunoblotting,for
R4427 T7054 T7027 punct ", ",separated
R4428 T7055 T7056 mark as,described
R4429 T7056 T7027 advcl described,separated
R4430 T7057 T7058 punct [,12
R4431 T7058 T7027 parataxis 12,separated
R4432 T7059 T7058 punct ],12
R4433 T7060 T7027 punct .,separated
R4434 T7062 T7063 nummod 16.5,%
R4435 T7063 T7064 compound %,gels
R4436 T7064 T7071 nsubjpass gels,used
R4437 T7065 T7066 compound tris,tricine
R4438 T7066 T7064 compound tricine,gels
R4439 T7067 T7066 punct -,tricine
R4440 T7068 T7069 compound SDS,PAGE
R4441 T7069 T7064 compound PAGE,gels
R4442 T7070 T7069 punct -,PAGE
R4443 T7072 T7071 auxpass were,used
R4444 T7073 T7071 prep for,used
R4445 T7074 T7075 compound subunit,c
R4446 T7075 T7076 compound c,experiments
R4447 T7076 T7073 pobj experiments,for
R4448 T7077 T7076 compound immunoblotting,experiments
R4449 T7078 T7071 punct .,used

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T6623 59-64 CL_GO_EXT:cell denotes cells
T6624 103-108 CL_GO_EXT:cell denotes cells
T6625 147-152 GO:0040007 denotes grown
T6626 230-242 CHEBI:16842 denotes formaldehyde
T6627 292-300 CHEBI:17790 denotes methanol
T6628 301-308 CHEBI:15347 denotes acetone
T6629 363-371 GO:0042571 denotes antibody
T6630 384-389 CL_GO_EXT:cell denotes Cells
T6631 518-525 CHEBI_SO_EXT:glycine denotes glycine
T6632 563-571 GO:0042571 denotes antibody
T6633 583-595 CHEBI:9750 denotes Triton X-100
T6634 641-647 NCBITaxon:27592 denotes bovine
T6635 648-653 UBERON:0001977 denotes serum
T6636 648-661 PR_EXT:000003918 denotes serum albumin
T6637 699-707 GO:0042571 denotes antibody
T6638 756-764 GO:0042571 denotes antibody
T6639 1074-1083 SO_EXT:wild_type_entity_or_quality denotes wild-type
T6640 1088-1094 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T6641 1127-1136 SO_EXT:wild_type_entity_or_quality denotes wild-type
T6642 1141-1147 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T6643 1249-1257 CHEBI_PR_EXT:protein denotes proteins
T6644 1277-1281 CL_GO_EXT:cell denotes cell
T6645 1324-1328 CHEBI:9754 denotes Tris
T6646 1341-1353 CHEBI:9750 denotes Triton X-100
T6647 1355-1363 GO:0016020 denotes membrane
T6648 1393-1412 CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor denotes protease inhibitors
T6649 1446-1457 CHEBI:7989 denotes pepstatin A
T6650 1486-1495 CHEBI:6426 denotes leupeptin
T6651 1674-1681 CHEBI_PR_EXT:protein denotes protein
T6652 1708-1715 CHEBI_PR_EXT:protein denotes Protein
T6653 1740-1743 CHEBI:8984 denotes SDS
T6654 1806-1810 CHEBI:9754 denotes tris
T6655 1811-1818 CHEBI:46760 denotes tricine
T6656 1819-1822 CHEBI:8984 denotes SDS
T6657 1847-1856 PR_EXT:000022190 denotes subunit c

2_test

Id Subject Object Predicate Lexical cue
15588329-12374761-12950169 1795-1797 12374761 denotes 12