Id |
Subject |
Object |
Predicate |
Lexical cue |
T6658 |
0-14 |
NN |
denotes |
Immunostaining |
T6659 |
30-38 |
NN |
denotes |
analysis |
T6660 |
15-18 |
CC |
denotes |
and |
T6661 |
19-29 |
NN |
denotes |
Immunoblot |
T6662 |
38-199 |
sentence |
denotes |
For immunostaining, cells were seeded at a density of 3–5 × 104 cells per well in 4-well chamber-slides and grown overnight at 33°C, unless indicated otherwise. |
T6663 |
39-42 |
IN |
denotes |
For |
T6664 |
70-76 |
VBN |
denotes |
seeded |
T6665 |
43-57 |
NN |
denotes |
immunostaining |
T6666 |
57-59 |
, |
denotes |
, |
T6667 |
59-64 |
NNS |
denotes |
cells |
T6668 |
65-69 |
VBD |
denotes |
were |
T6669 |
77-79 |
IN |
denotes |
at |
T6670 |
80-81 |
DT |
denotes |
a |
T6671 |
82-89 |
NN |
denotes |
density |
T6672 |
90-92 |
IN |
denotes |
of |
T6673 |
93-94 |
CD |
denotes |
3 |
T6674 |
95-96 |
CD |
denotes |
5 |
T6675 |
94-95 |
SYM |
denotes |
– |
T6676 |
99-102 |
CD |
denotes |
104 |
T6677 |
97-98 |
SYM |
denotes |
× |
T6678 |
103-108 |
NNS |
denotes |
cells |
T6679 |
109-112 |
IN |
denotes |
per |
T6680 |
113-117 |
NN |
denotes |
well |
T6681 |
118-120 |
IN |
denotes |
in |
T6682 |
121-122 |
CD |
denotes |
4 |
T6683 |
123-127 |
NN |
denotes |
well |
T6684 |
122-123 |
HYPH |
denotes |
- |
T6685 |
136-142 |
NNS |
denotes |
slides |
T6686 |
128-135 |
NN |
denotes |
chamber |
T6687 |
135-136 |
HYPH |
denotes |
- |
T6688 |
143-146 |
CC |
denotes |
and |
T6689 |
147-152 |
VBN |
denotes |
grown |
T6690 |
153-162 |
RB |
denotes |
overnight |
T6691 |
163-165 |
IN |
denotes |
at |
T6692 |
166-168 |
CD |
denotes |
33 |
T6693 |
168-170 |
NN |
denotes |
°C |
T6694 |
170-172 |
, |
denotes |
, |
T6695 |
172-178 |
IN |
denotes |
unless |
T6696 |
179-188 |
VBN |
denotes |
indicated |
T6697 |
189-198 |
RB |
denotes |
otherwise |
T6698 |
198-199 |
. |
denotes |
. |
T6699 |
199-383 |
sentence |
denotes |
Fixation was with ice-cold 4% formaldehyde in PBS, pH 7.4, for 20 minutes, or with ice-cold methanol/acetone (1:1) for 10 minutes at -20°C followed by air-drying (antibody-dependent). |
T6700 |
200-208 |
NN |
denotes |
Fixation |
T6701 |
209-212 |
VBD |
denotes |
was |
T6702 |
213-217 |
IN |
denotes |
with |
T6703 |
218-221 |
NN |
denotes |
ice |
T6704 |
222-226 |
JJ |
denotes |
cold |
T6705 |
221-222 |
HYPH |
denotes |
- |
T6706 |
230-242 |
NN |
denotes |
formaldehyde |
T6707 |
227-228 |
CD |
denotes |
4 |
T6708 |
228-229 |
NN |
denotes |
% |
T6709 |
243-245 |
IN |
denotes |
in |
T6710 |
246-249 |
NN |
denotes |
PBS |
T6711 |
249-251 |
, |
denotes |
, |
T6712 |
251-253 |
NN |
denotes |
pH |
T6713 |
254-257 |
CD |
denotes |
7.4 |
T6714 |
257-259 |
, |
denotes |
, |
T6715 |
259-262 |
IN |
denotes |
for |
T6716 |
263-265 |
CD |
denotes |
20 |
T6717 |
266-273 |
NNS |
denotes |
minutes |
T6718 |
273-275 |
, |
denotes |
, |
T6719 |
275-277 |
CC |
denotes |
or |
T6720 |
278-282 |
IN |
denotes |
with |
T6721 |
283-286 |
NN |
denotes |
ice |
T6722 |
287-291 |
JJ |
denotes |
cold |
T6723 |
286-287 |
HYPH |
denotes |
- |
T6724 |
301-308 |
NN |
denotes |
acetone |
T6725 |
292-300 |
NN |
denotes |
methanol |
T6726 |
300-301 |
HYPH |
denotes |
/ |
T6727 |
309-310 |
-LRB- |
denotes |
( |
T6728 |
310-311 |
CD |
denotes |
1 |
T6729 |
311-312 |
SYM |
denotes |
: |
T6730 |
312-313 |
CD |
denotes |
1 |
T6731 |
313-314 |
-RRB- |
denotes |
) |
T6732 |
315-318 |
IN |
denotes |
for |
T6733 |
319-321 |
CD |
denotes |
10 |
T6734 |
322-329 |
NNS |
denotes |
minutes |
T6735 |
330-332 |
IN |
denotes |
at |
T6736 |
333-334 |
SYM |
denotes |
- |
T6737 |
334-336 |
CD |
denotes |
20 |
T6738 |
336-338 |
NN |
denotes |
°C |
T6739 |
339-347 |
VBN |
denotes |
followed |
T6740 |
348-350 |
IN |
denotes |
by |
T6741 |
351-354 |
NN |
denotes |
air |
T6742 |
355-361 |
NN |
denotes |
drying |
T6743 |
354-355 |
HYPH |
denotes |
- |
T6744 |
362-363 |
-LRB- |
denotes |
( |
T6745 |
372-381 |
JJ |
denotes |
dependent |
T6746 |
363-371 |
NN |
denotes |
antibody |
T6747 |
371-372 |
HYPH |
denotes |
- |
T6748 |
381-382 |
-RRB- |
denotes |
) |
T6749 |
382-383 |
. |
denotes |
. |
T6750 |
383-802 |
sentence |
denotes |
Cells were washed with PBS at least 2 times, 5 minutes per wash, between each of the following steps of the staining procedure: 0.1 M glycine in PBS for 5 minutes, 0.05% or 0.1% (antibody-dependent) Triton X-100 (Fisher Scientific) in PBS for 5 minutes, 2% bovine serum albumin (BSA) in PBS for 30 minutes, primary antibody diluted in 2% BSA/PBS for 90 minutes, secondary antibody diluted in 2% BSA/PBS for 60 minutes. |
T6751 |
384-389 |
NNS |
denotes |
Cells |
T6752 |
395-401 |
VBN |
denotes |
washed |
T6753 |
390-394 |
VBD |
denotes |
were |
T6754 |
402-406 |
IN |
denotes |
with |
T6755 |
407-410 |
NN |
denotes |
PBS |
T6756 |
411-413 |
RB |
denotes |
at |
T6757 |
420-421 |
CD |
denotes |
2 |
T6758 |
414-419 |
RBS |
denotes |
least |
T6759 |
422-427 |
NNS |
denotes |
times |
T6760 |
427-429 |
, |
denotes |
, |
T6761 |
429-430 |
CD |
denotes |
5 |
T6762 |
431-438 |
NNS |
denotes |
minutes |
T6763 |
439-442 |
IN |
denotes |
per |
T6764 |
443-447 |
NN |
denotes |
wash |
T6765 |
447-449 |
, |
denotes |
, |
T6766 |
449-456 |
IN |
denotes |
between |
T6767 |
457-461 |
DT |
denotes |
each |
T6768 |
462-464 |
IN |
denotes |
of |
T6769 |
465-468 |
DT |
denotes |
the |
T6770 |
479-484 |
NNS |
denotes |
steps |
T6771 |
469-478 |
JJ |
denotes |
following |
T6772 |
485-487 |
IN |
denotes |
of |
T6773 |
488-491 |
DT |
denotes |
the |
T6774 |
501-510 |
NN |
denotes |
procedure |
T6775 |
492-500 |
NN |
denotes |
staining |
T6776 |
510-512 |
: |
denotes |
: |
T6777 |
512-515 |
CD |
denotes |
0.1 |
T6778 |
516-517 |
NN |
denotes |
M |
T6779 |
518-525 |
NN |
denotes |
glycine |
T6780 |
526-528 |
IN |
denotes |
in |
T6781 |
529-532 |
NN |
denotes |
PBS |
T6782 |
533-536 |
IN |
denotes |
for |
T6783 |
537-538 |
CD |
denotes |
5 |
T6784 |
539-546 |
NNS |
denotes |
minutes |
T6785 |
546-548 |
, |
denotes |
, |
T6786 |
548-552 |
CD |
denotes |
0.05 |
T6787 |
552-553 |
NN |
denotes |
% |
T6788 |
590-591 |
NN |
denotes |
X |
T6789 |
554-556 |
CC |
denotes |
or |
T6790 |
557-560 |
CD |
denotes |
0.1 |
T6791 |
560-561 |
NN |
denotes |
% |
T6792 |
562-563 |
-LRB- |
denotes |
( |
T6793 |
563-571 |
NN |
denotes |
antibody |
T6794 |
572-581 |
JJ |
denotes |
dependent |
T6795 |
571-572 |
HYPH |
denotes |
- |
T6796 |
581-582 |
-RRB- |
denotes |
) |
T6797 |
583-589 |
NN |
denotes |
Triton |
T6798 |
591-592 |
HYPH |
denotes |
- |
T6799 |
592-595 |
CD |
denotes |
100 |
T6800 |
596-597 |
-LRB- |
denotes |
( |
T6801 |
604-614 |
NNP |
denotes |
Scientific |
T6802 |
597-603 |
NNP |
denotes |
Fisher |
T6803 |
614-615 |
-RRB- |
denotes |
) |
T6804 |
616-618 |
IN |
denotes |
in |
T6805 |
619-622 |
NN |
denotes |
PBS |
T6806 |
623-626 |
IN |
denotes |
for |
T6807 |
627-628 |
CD |
denotes |
5 |
T6808 |
629-636 |
NNS |
denotes |
minutes |
T6809 |
636-638 |
, |
denotes |
, |
T6810 |
638-639 |
CD |
denotes |
2 |
T6811 |
639-640 |
NN |
denotes |
% |
T6812 |
641-647 |
JJ |
denotes |
bovine |
T6813 |
654-661 |
NN |
denotes |
albumin |
T6814 |
648-653 |
NN |
denotes |
serum |
T6815 |
662-663 |
-LRB- |
denotes |
( |
T6816 |
663-666 |
NN |
denotes |
BSA |
T6817 |
666-667 |
-RRB- |
denotes |
) |
T6818 |
668-670 |
IN |
denotes |
in |
T6819 |
671-674 |
NN |
denotes |
PBS |
T6820 |
675-678 |
IN |
denotes |
for |
T6821 |
679-681 |
CD |
denotes |
30 |
T6822 |
682-689 |
NNS |
denotes |
minutes |
T6823 |
689-691 |
, |
denotes |
, |
T6824 |
691-698 |
JJ |
denotes |
primary |
T6825 |
699-707 |
NN |
denotes |
antibody |
T6826 |
708-715 |
VBN |
denotes |
diluted |
T6827 |
716-718 |
IN |
denotes |
in |
T6828 |
719-720 |
CD |
denotes |
2 |
T6829 |
720-721 |
NN |
denotes |
% |
T6830 |
726-729 |
NN |
denotes |
PBS |
T6831 |
722-725 |
NN |
denotes |
BSA |
T6832 |
725-726 |
HYPH |
denotes |
/ |
T6833 |
730-733 |
IN |
denotes |
for |
T6834 |
734-736 |
CD |
denotes |
90 |
T6835 |
737-744 |
NNS |
denotes |
minutes |
T6836 |
744-746 |
, |
denotes |
, |
T6837 |
746-755 |
JJ |
denotes |
secondary |
T6838 |
756-764 |
NN |
denotes |
antibody |
T6839 |
765-772 |
VBN |
denotes |
diluted |
T6840 |
773-775 |
IN |
denotes |
in |
T6841 |
776-777 |
CD |
denotes |
2 |
T6842 |
777-778 |
NN |
denotes |
% |
T6843 |
783-786 |
NN |
denotes |
PBS |
T6844 |
779-782 |
NN |
denotes |
BSA |
T6845 |
782-783 |
HYPH |
denotes |
/ |
T6846 |
787-790 |
IN |
denotes |
for |
T6847 |
791-793 |
CD |
denotes |
60 |
T6848 |
794-801 |
NNS |
denotes |
minutes |
T6849 |
801-802 |
. |
denotes |
. |
T6850 |
802-856 |
sentence |
denotes |
All incubations were carried out at room temperature. |
T6851 |
803-806 |
DT |
denotes |
All |
T6852 |
807-818 |
NNS |
denotes |
incubations |
T6853 |
824-831 |
VBN |
denotes |
carried |
T6854 |
819-823 |
VBD |
denotes |
were |
T6855 |
832-835 |
RP |
denotes |
out |
T6856 |
836-838 |
IN |
denotes |
at |
T6857 |
839-843 |
NN |
denotes |
room |
T6858 |
844-855 |
NN |
denotes |
temperature |
T6859 |
855-856 |
. |
denotes |
. |
T6860 |
856-1107 |
sentence |
denotes |
Following staining procedures, slides were coverslipped with Vectashield mounting medium (Vector Laboratories) and analyzed on a BioRad Radiance 2100 confocal microscope (Biorad), with identical exposure settings for wild-type and mutant like images. |
T6861 |
857-866 |
VBG |
denotes |
Following |
T6862 |
900-912 |
VBN |
denotes |
coverslipped |
T6863 |
867-875 |
NN |
denotes |
staining |
T6864 |
876-886 |
NNS |
denotes |
procedures |
T6865 |
886-888 |
, |
denotes |
, |
T6866 |
888-894 |
NNS |
denotes |
slides |
T6867 |
895-899 |
VBD |
denotes |
were |
T6868 |
913-917 |
IN |
denotes |
with |
T6869 |
918-929 |
NNP |
denotes |
Vectashield |
T6870 |
939-945 |
NN |
denotes |
medium |
T6871 |
930-938 |
NN |
denotes |
mounting |
T6872 |
946-947 |
-LRB- |
denotes |
( |
T6873 |
954-966 |
NNP |
denotes |
Laboratories |
T6874 |
947-953 |
NNP |
denotes |
Vector |
T6875 |
966-967 |
-RRB- |
denotes |
) |
T6876 |
968-971 |
CC |
denotes |
and |
T6877 |
972-980 |
VBN |
denotes |
analyzed |
T6878 |
981-983 |
IN |
denotes |
on |
T6879 |
984-985 |
DT |
denotes |
a |
T6880 |
1016-1026 |
NN |
denotes |
microscope |
T6881 |
986-992 |
NNP |
denotes |
BioRad |
T6882 |
993-1001 |
NNP |
denotes |
Radiance |
T6883 |
1002-1006 |
CD |
denotes |
2100 |
T6884 |
1007-1015 |
JJ |
denotes |
confocal |
T6885 |
1027-1028 |
-LRB- |
denotes |
( |
T6886 |
1028-1034 |
NNP |
denotes |
Biorad |
T6887 |
1034-1035 |
-RRB- |
denotes |
) |
T6888 |
1035-1037 |
, |
denotes |
, |
T6889 |
1037-1041 |
IN |
denotes |
with |
T6890 |
1042-1051 |
JJ |
denotes |
identical |
T6891 |
1061-1069 |
NNS |
denotes |
settings |
T6892 |
1052-1060 |
NN |
denotes |
exposure |
T6893 |
1070-1073 |
IN |
denotes |
for |
T6894 |
1074-1078 |
JJ |
denotes |
wild |
T6895 |
1079-1083 |
NN |
denotes |
type |
T6896 |
1078-1079 |
HYPH |
denotes |
- |
T6897 |
1100-1106 |
NNS |
denotes |
images |
T6898 |
1084-1087 |
CC |
denotes |
and |
T6899 |
1088-1094 |
JJ |
denotes |
mutant |
T6900 |
1095-1099 |
JJ |
denotes |
like |
T6901 |
1106-1107 |
. |
denotes |
. |
T6902 |
1107-1242 |
sentence |
denotes |
All comparisons of wild-type and mutant staining were performed in Adobe Photoshop with identical brightness and contrast adjustments. |
T6903 |
1108-1111 |
DT |
denotes |
All |
T6904 |
1112-1123 |
NNS |
denotes |
comparisons |
T6905 |
1162-1171 |
VBN |
denotes |
performed |
T6906 |
1124-1126 |
IN |
denotes |
of |
T6907 |
1127-1131 |
JJ |
denotes |
wild |
T6908 |
1132-1136 |
NN |
denotes |
type |
T6909 |
1131-1132 |
HYPH |
denotes |
- |
T6910 |
1148-1156 |
NN |
denotes |
staining |
T6911 |
1137-1140 |
CC |
denotes |
and |
T6912 |
1141-1147 |
NN |
denotes |
mutant |
T6913 |
1157-1161 |
VBD |
denotes |
were |
T6914 |
1172-1174 |
IN |
denotes |
in |
T6915 |
1175-1180 |
NNP |
denotes |
Adobe |
T6916 |
1181-1190 |
NNP |
denotes |
Photoshop |
T6917 |
1191-1195 |
IN |
denotes |
with |
T6918 |
1196-1205 |
JJ |
denotes |
identical |
T6919 |
1230-1241 |
NNS |
denotes |
adjustments |
T6920 |
1206-1216 |
NN |
denotes |
brightness |
T6921 |
1217-1220 |
CC |
denotes |
and |
T6922 |
1221-1229 |
NN |
denotes |
contrast |
T6923 |
1241-1242 |
. |
denotes |
. |
T6924 |
1242-1505 |
sentence |
denotes |
Total proteins were isolated from cell pellets by extraction with ice-cold 20 mM Tris, pH 7.4, 1% Triton X-100 (membrane-research grade, Roche), plus protease inhibitors (Complete mini tablet, 0.7 μg/ml pepstatin A, 2 μg/ml aprotinin, 5 μg/ml leupeptin [Roche]). |
T6925 |
1243-1248 |
JJ |
denotes |
Total |
T6926 |
1249-1257 |
NN |
denotes |
proteins |
T6927 |
1263-1271 |
VBN |
denotes |
isolated |
T6928 |
1258-1262 |
VBD |
denotes |
were |
T6929 |
1272-1276 |
IN |
denotes |
from |
T6930 |
1277-1281 |
NN |
denotes |
cell |
T6931 |
1282-1289 |
NNS |
denotes |
pellets |
T6932 |
1290-1292 |
IN |
denotes |
by |
T6933 |
1293-1303 |
NN |
denotes |
extraction |
T6934 |
1304-1308 |
IN |
denotes |
with |
T6935 |
1309-1312 |
NN |
denotes |
ice |
T6936 |
1324-1328 |
NN |
denotes |
Tris |
T6937 |
1312-1313 |
HYPH |
denotes |
- |
T6938 |
1313-1317 |
JJ |
denotes |
cold |
T6939 |
1318-1320 |
CD |
denotes |
20 |
T6940 |
1321-1323 |
NN |
denotes |
mM |
T6941 |
1328-1330 |
, |
denotes |
, |
T6942 |
1330-1332 |
NN |
denotes |
pH |
T6943 |
1333-1336 |
CD |
denotes |
7.4 |
T6944 |
1336-1338 |
, |
denotes |
, |
T6945 |
1338-1339 |
CD |
denotes |
1 |
T6946 |
1339-1340 |
NN |
denotes |
% |
T6947 |
1348-1349 |
NN |
denotes |
X |
T6948 |
1341-1347 |
NN |
denotes |
Triton |
T6949 |
1349-1350 |
HYPH |
denotes |
- |
T6950 |
1350-1353 |
CD |
denotes |
100 |
T6951 |
1354-1355 |
-LRB- |
denotes |
( |
T6952 |
1380-1385 |
NNP |
denotes |
Roche |
T6953 |
1355-1363 |
NN |
denotes |
membrane |
T6954 |
1364-1372 |
NN |
denotes |
research |
T6955 |
1363-1364 |
HYPH |
denotes |
- |
T6956 |
1373-1378 |
NN |
denotes |
grade |
T6957 |
1378-1380 |
, |
denotes |
, |
T6958 |
1385-1386 |
-RRB- |
denotes |
) |
T6959 |
1386-1388 |
, |
denotes |
, |
T6960 |
1388-1392 |
CC |
denotes |
plus |
T6961 |
1393-1401 |
NN |
denotes |
protease |
T6962 |
1402-1412 |
NNS |
denotes |
inhibitors |
T6963 |
1413-1414 |
-LRB- |
denotes |
( |
T6964 |
1414-1422 |
JJ |
denotes |
Complete |
T6965 |
1428-1434 |
NN |
denotes |
tablet |
T6966 |
1423-1427 |
NN |
denotes |
mini |
T6967 |
1434-1436 |
, |
denotes |
, |
T6968 |
1436-1439 |
CD |
denotes |
0.7 |
T6969 |
1440-1442 |
NN |
denotes |
μg |
T6970 |
1456-1457 |
NN |
denotes |
A |
T6971 |
1442-1443 |
SYM |
denotes |
/ |
T6972 |
1443-1445 |
NN |
denotes |
ml |
T6973 |
1446-1455 |
NN |
denotes |
pepstatin |
T6974 |
1457-1459 |
, |
denotes |
, |
T6975 |
1459-1460 |
CD |
denotes |
2 |
T6976 |
1461-1463 |
NN |
denotes |
μg |
T6977 |
1467-1476 |
NN |
denotes |
aprotinin |
T6978 |
1463-1464 |
SYM |
denotes |
/ |
T6979 |
1464-1466 |
NN |
denotes |
ml |
T6980 |
1476-1478 |
, |
denotes |
, |
T6981 |
1478-1479 |
CD |
denotes |
5 |
T6982 |
1480-1482 |
NN |
denotes |
μg |
T6983 |
1486-1495 |
NN |
denotes |
leupeptin |
T6984 |
1482-1483 |
SYM |
denotes |
/ |
T6985 |
1483-1485 |
NN |
denotes |
ml |
T6986 |
1496-1497 |
-LRB- |
denotes |
[ |
T6987 |
1497-1502 |
NNP |
denotes |
Roche |
T6988 |
1502-1503 |
-RRB- |
denotes |
] |
T6989 |
1503-1504 |
-RRB- |
denotes |
) |
T6990 |
1504-1505 |
. |
denotes |
. |
T6991 |
1505-1650 |
sentence |
denotes |
Following homogenization through a 25-gauge needle (~10 passes), extracts were centrifuged at 1000 × g for 10 minutes, at 4°C, to remove debris. |
T6992 |
1506-1515 |
VBG |
denotes |
Following |
T6993 |
1585-1596 |
VBN |
denotes |
centrifuged |
T6994 |
1516-1530 |
NN |
denotes |
homogenization |
T6995 |
1531-1538 |
IN |
denotes |
through |
T6996 |
1539-1540 |
DT |
denotes |
a |
T6997 |
1550-1556 |
NN |
denotes |
needle |
T6998 |
1541-1543 |
CD |
denotes |
25 |
T6999 |
1544-1549 |
NN |
denotes |
gauge |
T7000 |
1543-1544 |
HYPH |
denotes |
- |
T7001 |
1557-1558 |
-LRB- |
denotes |
( |
T7002 |
1562-1568 |
NNS |
denotes |
passes |
T7003 |
1558-1559 |
SYM |
denotes |
~ |
T7004 |
1559-1561 |
CD |
denotes |
10 |
T7005 |
1568-1569 |
-RRB- |
denotes |
) |
T7006 |
1569-1571 |
, |
denotes |
, |
T7007 |
1571-1579 |
NNS |
denotes |
extracts |
T7008 |
1580-1584 |
VBD |
denotes |
were |
T7009 |
1597-1599 |
IN |
denotes |
at |
T7010 |
1600-1604 |
CD |
denotes |
1000 |
T7011 |
1607-1608 |
NN |
denotes |
g |
T7012 |
1605-1606 |
SYM |
denotes |
× |
T7013 |
1609-1612 |
IN |
denotes |
for |
T7014 |
1613-1615 |
CD |
denotes |
10 |
T7015 |
1616-1623 |
NNS |
denotes |
minutes |
T7016 |
1623-1625 |
, |
denotes |
, |
T7017 |
1625-1627 |
IN |
denotes |
at |
T7018 |
1628-1629 |
CD |
denotes |
4 |
T7019 |
1629-1631 |
NN |
denotes |
°C |
T7020 |
1631-1633 |
, |
denotes |
, |
T7021 |
1633-1635 |
TO |
denotes |
to |
T7022 |
1636-1642 |
VB |
denotes |
remove |
T7023 |
1643-1649 |
NN |
denotes |
debris |
T7024 |
1649-1650 |
. |
denotes |
. |
T7025 |
1650-1799 |
sentence |
denotes |
Typically, 20–40 μg of protein (determined by Bio-rad Dc Protein Assay) was separated by SDS-PAGE, for subsequent immunoblotting, as described [12]. |
T7026 |
1651-1660 |
RB |
denotes |
Typically |
T7027 |
1727-1736 |
VBN |
denotes |
separated |
T7028 |
1660-1662 |
, |
denotes |
, |
T7029 |
1662-1664 |
CD |
denotes |
20 |
T7030 |
1665-1667 |
CD |
denotes |
40 |
T7031 |
1664-1665 |
SYM |
denotes |
– |
T7032 |
1668-1670 |
NN |
denotes |
μg |
T7033 |
1671-1673 |
IN |
denotes |
of |
T7034 |
1674-1681 |
NN |
denotes |
protein |
T7035 |
1682-1683 |
-LRB- |
denotes |
( |
T7036 |
1683-1693 |
VBN |
denotes |
determined |
T7037 |
1694-1696 |
IN |
denotes |
by |
T7038 |
1697-1700 |
NNP |
denotes |
Bio |
T7039 |
1701-1704 |
NNP |
denotes |
rad |
T7040 |
1700-1701 |
HYPH |
denotes |
- |
T7041 |
1716-1721 |
NN |
denotes |
Assay |
T7042 |
1705-1707 |
NN |
denotes |
Dc |
T7043 |
1708-1715 |
NN |
denotes |
Protein |
T7044 |
1721-1722 |
-RRB- |
denotes |
) |
T7045 |
1723-1726 |
VBD |
denotes |
was |
T7046 |
1737-1739 |
IN |
denotes |
by |
T7047 |
1740-1743 |
NN |
denotes |
SDS |
T7048 |
1744-1748 |
NN |
denotes |
PAGE |
T7049 |
1743-1744 |
HYPH |
denotes |
- |
T7050 |
1748-1750 |
, |
denotes |
, |
T7051 |
1750-1753 |
IN |
denotes |
for |
T7052 |
1754-1764 |
JJ |
denotes |
subsequent |
T7053 |
1765-1779 |
NN |
denotes |
immunoblotting |
T7054 |
1779-1781 |
, |
denotes |
, |
T7055 |
1781-1783 |
IN |
denotes |
as |
T7056 |
1784-1793 |
VBN |
denotes |
described |
T7057 |
1794-1795 |
-LRB- |
denotes |
[ |
T7058 |
1795-1797 |
CD |
denotes |
12 |
T7059 |
1797-1798 |
-RRB- |
denotes |
] |
T7060 |
1798-1799 |
. |
denotes |
. |
T7061 |
1799-1884 |
sentence |
denotes |
16.5% tris-tricine SDS-PAGE gels were used for subunit c immunoblotting experiments. |
T7062 |
1800-1804 |
CD |
denotes |
16.5 |
T7063 |
1804-1805 |
NN |
denotes |
% |
T7064 |
1828-1832 |
NNS |
denotes |
gels |
T7065 |
1806-1810 |
NN |
denotes |
tris |
T7066 |
1811-1818 |
NN |
denotes |
tricine |
T7067 |
1810-1811 |
HYPH |
denotes |
- |
T7068 |
1819-1822 |
NN |
denotes |
SDS |
T7069 |
1823-1827 |
NN |
denotes |
PAGE |
T7070 |
1822-1823 |
HYPH |
denotes |
- |
T7071 |
1838-1842 |
VBN |
denotes |
used |
T7072 |
1833-1837 |
VBD |
denotes |
were |
T7073 |
1843-1846 |
IN |
denotes |
for |
T7074 |
1847-1854 |
NN |
denotes |
subunit |
T7075 |
1855-1856 |
NN |
denotes |
c |
T7076 |
1872-1883 |
NNS |
denotes |
experiments |
T7077 |
1857-1871 |
NN |
denotes |
immunoblotting |
T7078 |
1883-1884 |
. |
denotes |
. |
R4100 |
T6713 |
T6712 |
nummod |
7.4,pH |
R4101 |
T6714 |
T6706 |
punct |
", ",formaldehyde |
R4102 |
T6715 |
T6706 |
prep |
for,formaldehyde |
R4103 |
T6716 |
T6717 |
nummod |
20,minutes |
R4104 |
T6717 |
T6715 |
pobj |
minutes,for |
R4105 |
T6718 |
T6702 |
punct |
", ",with |
R4106 |
T6719 |
T6702 |
cc |
or,with |
R4108 |
T6721 |
T6722 |
npadvmod |
ice,cold |
R4109 |
T6722 |
T6724 |
amod |
cold,acetone |
R4110 |
T6723 |
T6722 |
punct |
-,cold |
R4111 |
T6724 |
T6720 |
pobj |
acetone,with |
R4112 |
T6725 |
T6724 |
compound |
methanol,acetone |
R4113 |
T6726 |
T6724 |
punct |
/,acetone |
R4114 |
T6727 |
T6728 |
punct |
(,1 |
R4115 |
T6728 |
T6724 |
parataxis |
1,acetone |
R4116 |
T6729 |
T6730 |
punct |
:,1 |
R4117 |
T6730 |
T6728 |
prep |
1,1 |
R4252 |
T6871 |
T6870 |
compound |
mounting,medium |
R4253 |
T6872 |
T6873 |
punct |
(,Laboratories |
R4254 |
T6873 |
T6870 |
parataxis |
Laboratories,medium |
R4255 |
T6874 |
T6873 |
compound |
Vector,Laboratories |
R4256 |
T6875 |
T6873 |
punct |
),Laboratories |
R4257 |
T6876 |
T6862 |
cc |
and,coverslipped |
R4258 |
T6877 |
T6862 |
conj |
analyzed,coverslipped |
R4259 |
T6878 |
T6877 |
prep |
on,analyzed |
R4260 |
T6879 |
T6880 |
det |
a,microscope |
R4261 |
T6880 |
T6878 |
pobj |
microscope,on |
R4262 |
T6881 |
T6882 |
nmod |
BioRad,Radiance |
R4263 |
T6882 |
T6880 |
nmod |
Radiance,microscope |
R4264 |
T6883 |
T6882 |
nummod |
2100,Radiance |
R4050 |
T6658 |
T6659 |
nmod |
Immunostaining,analysis |
R4051 |
T6660 |
T6658 |
cc |
and,Immunostaining |
R4052 |
T6661 |
T6658 |
conj |
Immunoblot,Immunostaining |
R4053 |
T6663 |
T6664 |
prep |
For,seeded |
R4054 |
T6665 |
T6663 |
pobj |
immunostaining,For |
R4055 |
T6666 |
T6664 |
punct |
", ",seeded |
R4056 |
T6667 |
T6664 |
nsubj |
cells,seeded |
R4057 |
T6668 |
T6664 |
aux |
were,seeded |
R4058 |
T6669 |
T6664 |
prep |
at,seeded |
R4059 |
T6670 |
T6671 |
det |
a,density |
R4060 |
T6671 |
T6669 |
pobj |
density,at |
R4061 |
T6672 |
T6671 |
prep |
of,density |
R4062 |
T6673 |
T6674 |
quantmod |
3,5 |
R4063 |
T6674 |
T6676 |
quantmod |
5,104 |
R4064 |
T6675 |
T6674 |
punct |
–,5 |
R4065 |
T6676 |
T6678 |
nummod |
104,cells |
R4066 |
T6677 |
T6676 |
punct |
×,104 |
R4067 |
T6678 |
T6672 |
pobj |
cells,of |
R4068 |
T6679 |
T6678 |
prep |
per,cells |
R4069 |
T6680 |
T6679 |
pobj |
well,per |
R4070 |
T6681 |
T6664 |
prep |
in,seeded |
R4071 |
T6682 |
T6683 |
nummod |
4,well |
R4072 |
T6683 |
T6685 |
compound |
well,slides |
R4073 |
T6684 |
T6683 |
punct |
-,well |
R4074 |
T6685 |
T6681 |
pobj |
slides,in |
R4075 |
T6686 |
T6685 |
compound |
chamber,slides |
R4076 |
T6687 |
T6685 |
punct |
-,slides |
R4077 |
T6688 |
T6664 |
cc |
and,seeded |
R4078 |
T6689 |
T6664 |
conj |
grown,seeded |
R4079 |
T6690 |
T6689 |
advmod |
overnight,grown |
R4080 |
T6691 |
T6689 |
prep |
at,grown |
R4081 |
T6692 |
T6693 |
nummod |
33,°C |
R4082 |
T6693 |
T6691 |
pobj |
°C,at |
R4083 |
T6694 |
T6664 |
punct |
", ",seeded |
R4084 |
T6695 |
T6696 |
mark |
unless,indicated |
R4085 |
T6696 |
T6664 |
advcl |
indicated,seeded |
R4086 |
T6697 |
T6696 |
advmod |
otherwise,indicated |
R4087 |
T6698 |
T6664 |
punct |
.,seeded |
R4088 |
T6700 |
T6701 |
nsubj |
Fixation,was |
R4089 |
T6702 |
T6701 |
prep |
with,was |
R4090 |
T6703 |
T6704 |
npadvmod |
ice,cold |
R4091 |
T6704 |
T6706 |
amod |
cold,formaldehyde |
R4092 |
T6705 |
T6704 |
punct |
-,cold |
R4093 |
T6706 |
T6702 |
pobj |
formaldehyde,with |
R4094 |
T6707 |
T6708 |
nummod |
4,% |
R4095 |
T6708 |
T6706 |
compound |
%,formaldehyde |
R4096 |
T6709 |
T6706 |
prep |
in,formaldehyde |
R4097 |
T6710 |
T6709 |
pobj |
PBS,in |
R4098 |
T6711 |
T6710 |
punct |
", ",PBS |
R4099 |
T6712 |
T6710 |
npadvmod |
pH,PBS |
R4107 |
T6720 |
T6702 |
conj |
with,with |
R4118 |
T6731 |
T6728 |
punct |
),1 |
R4119 |
T6732 |
T6724 |
prep |
for,acetone |
R4120 |
T6733 |
T6734 |
nummod |
10,minutes |
R4121 |
T6734 |
T6732 |
pobj |
minutes,for |
R4122 |
T6735 |
T6724 |
prep |
at,acetone |
R4123 |
T6736 |
T6737 |
punct |
-,20 |
R4124 |
T6737 |
T6738 |
nummod |
20,°C |
R4125 |
T6738 |
T6735 |
pobj |
°C,at |
R4126 |
T6739 |
T6724 |
acl |
followed,acetone |
R4127 |
T6740 |
T6739 |
agent |
by,followed |
R4128 |
T6741 |
T6742 |
compound |
air,drying |
R4129 |
T6742 |
T6740 |
pobj |
drying,by |
R4130 |
T6743 |
T6742 |
punct |
-,drying |
R4131 |
T6744 |
T6745 |
punct |
(,dependent |
R4132 |
T6745 |
T6739 |
parataxis |
dependent,followed |
R4133 |
T6746 |
T6745 |
npadvmod |
antibody,dependent |
R4134 |
T6747 |
T6745 |
punct |
-,dependent |
R4135 |
T6748 |
T6745 |
punct |
),dependent |
R4136 |
T6749 |
T6701 |
punct |
.,was |
R4137 |
T6751 |
T6752 |
nsubjpass |
Cells,washed |
R4138 |
T6753 |
T6752 |
auxpass |
were,washed |
R4139 |
T6754 |
T6752 |
prep |
with,washed |
R4140 |
T6755 |
T6754 |
pobj |
PBS,with |
R4141 |
T6756 |
T6757 |
advmod |
at,2 |
R4142 |
T6757 |
T6752 |
npadvmod |
2,washed |
R4143 |
T6758 |
T6757 |
advmod |
least,2 |
R4144 |
T6759 |
T6757 |
quantmod |
times,2 |
R4145 |
T6760 |
T6752 |
punct |
", ",washed |
R4146 |
T6761 |
T6762 |
nummod |
5,minutes |
R4147 |
T6762 |
T6752 |
npadvmod |
minutes,washed |
R4148 |
T6763 |
T6762 |
prep |
per,minutes |
R4149 |
T6764 |
T6763 |
pobj |
wash,per |
R4150 |
T6765 |
T6752 |
punct |
", ",washed |
R4151 |
T6766 |
T6752 |
prep |
between,washed |
R4152 |
T6767 |
T6766 |
pobj |
each,between |
R4153 |
T6768 |
T6767 |
prep |
of,each |
R4154 |
T6769 |
T6770 |
det |
the,steps |
R4155 |
T6770 |
T6768 |
pobj |
steps,of |
R4156 |
T6771 |
T6770 |
amod |
following,steps |
R4157 |
T6772 |
T6770 |
prep |
of,steps |
R4158 |
T6773 |
T6774 |
det |
the,procedure |
R4159 |
T6774 |
T6772 |
pobj |
procedure,of |
R4160 |
T6775 |
T6774 |
compound |
staining,procedure |
R4161 |
T6776 |
T6770 |
punct |
: ,steps |
R4162 |
T6777 |
T6778 |
nummod |
0.1,M |
R4163 |
T6778 |
T6779 |
compound |
M,glycine |
R4164 |
T6779 |
T6770 |
appos |
glycine,steps |
R4165 |
T6780 |
T6779 |
prep |
in,glycine |
R4166 |
T6781 |
T6780 |
pobj |
PBS,in |
R4167 |
T6782 |
T6779 |
prep |
for,glycine |
R4168 |
T6783 |
T6784 |
nummod |
5,minutes |
R4169 |
T6784 |
T6782 |
pobj |
minutes,for |
R4170 |
T6785 |
T6779 |
punct |
", ",glycine |
R4171 |
T6786 |
T6787 |
nummod |
0.05,% |
R4172 |
T6787 |
T6788 |
nmod |
%,X |
R4173 |
T6788 |
T6779 |
appos |
X,glycine |
R4174 |
T6789 |
T6787 |
cc |
or,% |
R4175 |
T6790 |
T6791 |
nummod |
0.1,% |
R4176 |
T6791 |
T6787 |
conj |
%,% |
R4177 |
T6792 |
T6788 |
punct |
(,X |
R4178 |
T6793 |
T6794 |
npadvmod |
antibody,dependent |
R4179 |
T6794 |
T6788 |
amod |
dependent,X |
R4180 |
T6795 |
T6794 |
punct |
-,dependent |
R4181 |
T6796 |
T6788 |
punct |
),X |
R4182 |
T6797 |
T6788 |
compound |
Triton,X |
R4183 |
T6798 |
T6788 |
punct |
-,X |
R4184 |
T6799 |
T6788 |
nummod |
100,X |
R4185 |
T6800 |
T6801 |
punct |
(,Scientific |
R4186 |
T6801 |
T6788 |
parataxis |
Scientific,X |
R4187 |
T6802 |
T6801 |
compound |
Fisher,Scientific |
R4188 |
T6803 |
T6801 |
punct |
),Scientific |
R4189 |
T6804 |
T6788 |
prep |
in,X |
R4190 |
T6805 |
T6804 |
pobj |
PBS,in |
R4191 |
T6806 |
T6788 |
prep |
for,X |
R4192 |
T6807 |
T6808 |
nummod |
5,minutes |
R4193 |
T6808 |
T6806 |
pobj |
minutes,for |
R4194 |
T6809 |
T6779 |
punct |
", ",glycine |
R4195 |
T6810 |
T6811 |
nummod |
2,% |
R4196 |
T6811 |
T6779 |
appos |
%,glycine |
R4197 |
T6812 |
T6813 |
amod |
bovine,albumin |
R4198 |
T6813 |
T6811 |
appos |
albumin,% |
R4199 |
T6814 |
T6813 |
compound |
serum,albumin |
R4200 |
T6815 |
T6813 |
punct |
(,albumin |
R4201 |
T6816 |
T6813 |
appos |
BSA,albumin |
R4202 |
T6817 |
T6811 |
punct |
),% |
R4203 |
T6818 |
T6811 |
prep |
in,% |
R4204 |
T6819 |
T6818 |
pobj |
PBS,in |
R4205 |
T6820 |
T6811 |
prep |
for,% |
R4206 |
T6821 |
T6822 |
nummod |
30,minutes |
R4207 |
T6822 |
T6820 |
pobj |
minutes,for |
R4208 |
T6823 |
T6779 |
punct |
", ",glycine |
R4209 |
T6824 |
T6825 |
amod |
primary,antibody |
R4210 |
T6825 |
T6779 |
appos |
antibody,glycine |
R4211 |
T6826 |
T6825 |
acl |
diluted,antibody |
R4212 |
T6827 |
T6826 |
prep |
in,diluted |
R4213 |
T6828 |
T6829 |
nummod |
2,% |
R4214 |
T6829 |
T6830 |
compound |
%,PBS |
R4215 |
T6830 |
T6827 |
pobj |
PBS,in |
R4216 |
T6831 |
T6830 |
compound |
BSA,PBS |
R4217 |
T6832 |
T6830 |
punct |
/,PBS |
R4218 |
T6833 |
T6826 |
prep |
for,diluted |
R4219 |
T6834 |
T6835 |
nummod |
90,minutes |
R4220 |
T6835 |
T6833 |
pobj |
minutes,for |
R4221 |
T6836 |
T6779 |
punct |
", ",glycine |
R4222 |
T6837 |
T6838 |
amod |
secondary,antibody |
R4223 |
T6838 |
T6779 |
appos |
antibody,glycine |
R4224 |
T6839 |
T6838 |
acl |
diluted,antibody |
R4225 |
T6840 |
T6839 |
prep |
in,diluted |
R4226 |
T6841 |
T6842 |
nummod |
2,% |
R4227 |
T6842 |
T6843 |
compound |
%,PBS |
R4228 |
T6843 |
T6840 |
pobj |
PBS,in |
R4229 |
T6844 |
T6843 |
compound |
BSA,PBS |
R4230 |
T6845 |
T6843 |
punct |
/,PBS |
R4231 |
T6846 |
T6839 |
prep |
for,diluted |
R4232 |
T6847 |
T6848 |
nummod |
60,minutes |
R4233 |
T6848 |
T6846 |
pobj |
minutes,for |
R4234 |
T6849 |
T6752 |
punct |
.,washed |
R4235 |
T6851 |
T6852 |
det |
All,incubations |
R4236 |
T6852 |
T6853 |
nsubjpass |
incubations,carried |
R4237 |
T6854 |
T6853 |
auxpass |
were,carried |
R4238 |
T6855 |
T6853 |
prt |
out,carried |
R4239 |
T6856 |
T6853 |
prep |
at,carried |
R4240 |
T6857 |
T6858 |
compound |
room,temperature |
R4241 |
T6858 |
T6856 |
pobj |
temperature,at |
R4242 |
T6859 |
T6853 |
punct |
.,carried |
R4243 |
T6861 |
T6862 |
prep |
Following,coverslipped |
R4244 |
T6863 |
T6864 |
compound |
staining,procedures |
R4245 |
T6864 |
T6861 |
pobj |
procedures,Following |
R4246 |
T6865 |
T6862 |
punct |
", ",coverslipped |
R4247 |
T6866 |
T6862 |
nsubjpass |
slides,coverslipped |
R4248 |
T6867 |
T6862 |
auxpass |
were,coverslipped |
R4249 |
T6868 |
T6862 |
prep |
with,coverslipped |
R4250 |
T6869 |
T6870 |
compound |
Vectashield,medium |
R4251 |
T6870 |
T6868 |
pobj |
medium,with |
R4265 |
T6884 |
T6880 |
amod |
confocal,microscope |
R4266 |
T6885 |
T6886 |
punct |
(,Biorad |
R4267 |
T6886 |
T6880 |
parataxis |
Biorad,microscope |
R4268 |
T6887 |
T6886 |
punct |
),Biorad |
R4269 |
T6888 |
T6877 |
punct |
", ",analyzed |
R4270 |
T6889 |
T6877 |
prep |
with,analyzed |
R4271 |
T6890 |
T6891 |
amod |
identical,settings |
R4272 |
T6891 |
T6889 |
pobj |
settings,with |
R4273 |
T6892 |
T6891 |
compound |
exposure,settings |
R4274 |
T6893 |
T6891 |
prep |
for,settings |
R4275 |
T6894 |
T6895 |
amod |
wild,type |
R4276 |
T6895 |
T6897 |
nmod |
type,images |
R4277 |
T6896 |
T6895 |
punct |
-,type |
R4278 |
T6897 |
T6893 |
pobj |
images,for |
R4279 |
T6898 |
T6895 |
cc |
and,type |
R4280 |
T6899 |
T6895 |
conj |
mutant,type |
R4281 |
T6900 |
T6897 |
amod |
like,images |
R4282 |
T6901 |
T6862 |
punct |
.,coverslipped |
R4283 |
T6903 |
T6904 |
det |
All,comparisons |
R4284 |
T6904 |
T6905 |
nsubjpass |
comparisons,performed |
R4285 |
T6906 |
T6904 |
prep |
of,comparisons |
R4286 |
T6907 |
T6908 |
amod |
wild,type |
R4287 |
T6908 |
T6910 |
nmod |
type,staining |
R4288 |
T6909 |
T6908 |
punct |
-,type |
R4289 |
T6910 |
T6906 |
pobj |
staining,of |
R4290 |
T6911 |
T6908 |
cc |
and,type |
R4291 |
T6912 |
T6908 |
conj |
mutant,type |
R4292 |
T6913 |
T6905 |
auxpass |
were,performed |
R4293 |
T6914 |
T6905 |
prep |
in,performed |
R4294 |
T6915 |
T6916 |
compound |
Adobe,Photoshop |
R4295 |
T6916 |
T6914 |
pobj |
Photoshop,in |
R4296 |
T6917 |
T6905 |
prep |
with,performed |
R4297 |
T6918 |
T6919 |
amod |
identical,adjustments |
R4298 |
T6919 |
T6917 |
pobj |
adjustments,with |
R4299 |
T6920 |
T6919 |
nmod |
brightness,adjustments |
R4300 |
T6921 |
T6920 |
cc |
and,brightness |
R4301 |
T6922 |
T6920 |
conj |
contrast,brightness |
R4302 |
T6923 |
T6905 |
punct |
.,performed |
R4303 |
T6925 |
T6926 |
amod |
Total,proteins |
R4304 |
T6926 |
T6927 |
nsubjpass |
proteins,isolated |
R4305 |
T6928 |
T6927 |
auxpass |
were,isolated |
R4306 |
T6929 |
T6927 |
prep |
from,isolated |
R4307 |
T6930 |
T6931 |
compound |
cell,pellets |
R4308 |
T6931 |
T6929 |
pobj |
pellets,from |
R4309 |
T6932 |
T6927 |
prep |
by,isolated |
R4310 |
T6933 |
T6932 |
pobj |
extraction,by |
R4311 |
T6934 |
T6933 |
prep |
with,extraction |
R4312 |
T6935 |
T6936 |
nmod |
ice,Tris |
R4313 |
T6936 |
T6934 |
pobj |
Tris,with |
R4314 |
T6937 |
T6935 |
punct |
-,ice |
R4315 |
T6938 |
T6935 |
amod |
cold,ice |
R4316 |
T6939 |
T6940 |
nummod |
20,mM |
R4317 |
T6940 |
T6936 |
compound |
mM,Tris |
R4318 |
T6941 |
T6936 |
punct |
", ",Tris |
R4319 |
T6942 |
T6936 |
npadvmod |
pH,Tris |
R4320 |
T6943 |
T6942 |
nummod |
7.4,pH |
R4321 |
T6944 |
T6936 |
punct |
", ",Tris |
R4322 |
T6945 |
T6946 |
nummod |
1,% |
R4323 |
T6946 |
T6947 |
compound |
%,X |
R4324 |
T6947 |
T6936 |
conj |
X,Tris |
R4325 |
T6948 |
T6947 |
compound |
Triton,X |
R4326 |
T6949 |
T6947 |
punct |
-,X |
R4327 |
T6950 |
T6947 |
nummod |
100,X |
R4328 |
T6951 |
T6952 |
punct |
(,Roche |
R4329 |
T6952 |
T6947 |
parataxis |
Roche,X |
R4330 |
T6953 |
T6954 |
compound |
membrane,research |
R4331 |
T6954 |
T6956 |
compound |
research,grade |
R4332 |
T6955 |
T6954 |
punct |
-,research |
R4333 |
T6956 |
T6952 |
dep |
grade,Roche |
R4334 |
T6957 |
T6952 |
punct |
", ",Roche |
R4335 |
T6958 |
T6952 |
punct |
),Roche |
R4336 |
T6959 |
T6947 |
punct |
", ",X |
R4337 |
T6960 |
T6947 |
cc |
plus,X |
R4338 |
T6961 |
T6962 |
compound |
protease,inhibitors |
R4339 |
T6962 |
T6947 |
conj |
inhibitors,X |
R4340 |
T6963 |
T6962 |
punct |
(,inhibitors |
R4341 |
T6964 |
T6965 |
amod |
Complete,tablet |
R4342 |
T6965 |
T6962 |
appos |
tablet,inhibitors |
R4343 |
T6966 |
T6965 |
compound |
mini,tablet |
R4344 |
T6967 |
T6965 |
punct |
", ",tablet |
R4345 |
T6968 |
T6969 |
nummod |
0.7,μg |
R4346 |
T6969 |
T6970 |
nmod |
μg,A |
R4347 |
T6970 |
T6965 |
appos |
A,tablet |
R4348 |
T6971 |
T6972 |
punct |
/,ml |
R4349 |
T6972 |
T6969 |
prep |
ml,μg |
R4350 |
T6973 |
T6970 |
compound |
pepstatin,A |
R4351 |
T6974 |
T6965 |
punct |
", ",tablet |
R4352 |
T6975 |
T6976 |
nummod |
2,μg |
R4353 |
T6976 |
T6977 |
nmod |
μg,aprotinin |
R4354 |
T6977 |
T6965 |
appos |
aprotinin,tablet |
R4355 |
T6978 |
T6979 |
punct |
/,ml |
R4356 |
T6979 |
T6976 |
prep |
ml,μg |
R4357 |
T6980 |
T6965 |
punct |
", ",tablet |
R4358 |
T6981 |
T6982 |
nummod |
5,μg |
R4359 |
T6982 |
T6983 |
nmod |
μg,leupeptin |
R4360 |
T6983 |
T6965 |
appos |
leupeptin,tablet |
R4361 |
T6984 |
T6985 |
punct |
/,ml |
R4362 |
T6985 |
T6982 |
prep |
ml,μg |
R4363 |
T6986 |
T6987 |
punct |
[,Roche |
R4364 |
T6987 |
T6983 |
parataxis |
Roche,leupeptin |
R4365 |
T6988 |
T6987 |
punct |
],Roche |
R4366 |
T6989 |
T6927 |
punct |
),isolated |
R4367 |
T6990 |
T6927 |
punct |
.,isolated |
R4368 |
T6992 |
T6993 |
prep |
Following,centrifuged |
R4369 |
T6994 |
T6992 |
pobj |
homogenization,Following |
R4370 |
T6995 |
T6994 |
prep |
through,homogenization |
R4371 |
T6996 |
T6997 |
det |
a,needle |
R4372 |
T6997 |
T6995 |
pobj |
needle,through |
R4373 |
T6998 |
T6999 |
nummod |
25,gauge |
R4374 |
T6999 |
T6997 |
compound |
gauge,needle |
R4375 |
T7000 |
T6999 |
punct |
-,gauge |
R4376 |
T7001 |
T7002 |
punct |
(,passes |
R4377 |
T7002 |
T6994 |
parataxis |
passes,homogenization |
R4378 |
T7003 |
T7004 |
punct |
~,10 |
R4379 |
T7004 |
T7002 |
nummod |
10,passes |
R4380 |
T7005 |
T7002 |
punct |
),passes |
R4381 |
T7006 |
T6993 |
punct |
", ",centrifuged |
R4382 |
T7007 |
T6993 |
nsubjpass |
extracts,centrifuged |
R4383 |
T7008 |
T6993 |
auxpass |
were,centrifuged |
R4384 |
T7009 |
T6993 |
prep |
at,centrifuged |
R4385 |
T7010 |
T7011 |
nummod |
1000,g |
R4386 |
T7011 |
T7009 |
pobj |
g,at |
R4387 |
T7012 |
T7011 |
punct |
×,g |
R4388 |
T7013 |
T6993 |
prep |
for,centrifuged |
R4389 |
T7014 |
T7015 |
nummod |
10,minutes |
R4390 |
T7015 |
T7013 |
pobj |
minutes,for |
R4391 |
T7016 |
T6993 |
punct |
", ",centrifuged |
R4392 |
T7017 |
T6993 |
prep |
at,centrifuged |
R4393 |
T7018 |
T7019 |
nummod |
4,°C |
R4394 |
T7019 |
T7017 |
pobj |
°C,at |
R4395 |
T7020 |
T6993 |
punct |
", ",centrifuged |
R4396 |
T7021 |
T7022 |
aux |
to,remove |
R4397 |
T7022 |
T6993 |
advcl |
remove,centrifuged |
R4398 |
T7023 |
T7022 |
dobj |
debris,remove |
R4399 |
T7024 |
T6993 |
punct |
.,centrifuged |
R4400 |
T7026 |
T7027 |
advmod |
Typically,separated |
R4401 |
T7028 |
T7027 |
punct |
", ",separated |
R4402 |
T7029 |
T7030 |
quantmod |
20,40 |
R4403 |
T7030 |
T7032 |
nummod |
40,μg |
R4404 |
T7031 |
T7030 |
punct |
–,40 |
R4405 |
T7032 |
T7027 |
nsubjpass |
μg,separated |
R4406 |
T7033 |
T7032 |
prep |
of,μg |
R4407 |
T7034 |
T7033 |
pobj |
protein,of |
R4408 |
T7035 |
T7034 |
punct |
(,protein |
R4409 |
T7036 |
T7034 |
acl |
determined,protein |
R4410 |
T7037 |
T7036 |
agent |
by,determined |
R4411 |
T7038 |
T7039 |
compound |
Bio,rad |
R4412 |
T7039 |
T7041 |
compound |
rad,Assay |
R4413 |
T7040 |
T7039 |
punct |
-,rad |
R4414 |
T7041 |
T7037 |
pobj |
Assay,by |
R4415 |
T7042 |
T7041 |
compound |
Dc,Assay |
R4416 |
T7043 |
T7041 |
compound |
Protein,Assay |
R4417 |
T7044 |
T7027 |
punct |
),separated |
R4418 |
T7045 |
T7027 |
auxpass |
was,separated |
R4419 |
T7046 |
T7027 |
prep |
by,separated |
R4420 |
T7047 |
T7048 |
compound |
SDS,PAGE |
R4421 |
T7048 |
T7046 |
pobj |
PAGE,by |
R4422 |
T7049 |
T7048 |
punct |
-,PAGE |
R4423 |
T7050 |
T7027 |
punct |
", ",separated |
R4424 |
T7051 |
T7027 |
prep |
for,separated |
R4425 |
T7052 |
T7053 |
amod |
subsequent,immunoblotting |
R4426 |
T7053 |
T7051 |
pobj |
immunoblotting,for |
R4427 |
T7054 |
T7027 |
punct |
", ",separated |
R4428 |
T7055 |
T7056 |
mark |
as,described |
R4429 |
T7056 |
T7027 |
advcl |
described,separated |
R4430 |
T7057 |
T7058 |
punct |
[,12 |
R4431 |
T7058 |
T7027 |
parataxis |
12,separated |
R4432 |
T7059 |
T7058 |
punct |
],12 |
R4433 |
T7060 |
T7027 |
punct |
.,separated |
R4434 |
T7062 |
T7063 |
nummod |
16.5,% |
R4435 |
T7063 |
T7064 |
compound |
%,gels |
R4436 |
T7064 |
T7071 |
nsubjpass |
gels,used |
R4437 |
T7065 |
T7066 |
compound |
tris,tricine |
R4438 |
T7066 |
T7064 |
compound |
tricine,gels |
R4439 |
T7067 |
T7066 |
punct |
-,tricine |
R4440 |
T7068 |
T7069 |
compound |
SDS,PAGE |
R4441 |
T7069 |
T7064 |
compound |
PAGE,gels |
R4442 |
T7070 |
T7069 |
punct |
-,PAGE |
R4443 |
T7072 |
T7071 |
auxpass |
were,used |
R4444 |
T7073 |
T7071 |
prep |
for,used |
R4445 |
T7074 |
T7075 |
compound |
subunit,c |
R4446 |
T7075 |
T7076 |
compound |
c,experiments |
R4447 |
T7076 |
T7073 |
pobj |
experiments,for |
R4448 |
T7077 |
T7076 |
compound |
immunoblotting,experiments |
R4449 |
T7078 |
T7071 |
punct |
.,used |