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PMC:539297 / 27274-29210 JSONTXT

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craft-ca-core-dev

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Id Subject Object Predicate Lexical cue
T5624 0-4 PR:000005591 denotes Cln3
T5625 20-24 NCBITaxon:10088 denotes mice
T5626 192-202 UBERON:0002037 denotes cerebellar
T5627 192-217 CL:0001031 denotes cerebellar granule neuron
T5628 246-251 GO:0007567 denotes natal
T5629 263-272 UBERON:0002037 denotes cerebella
T5630 360-367 CHEBI:17234 denotes glucose
T5631 369-373 UBERON:0002415 denotes Tail
T5632 407-414 SO:0001026 denotes genomic
T5633 453-460 PR:000027795 denotes Trypsin
T5634 553-562 UBERON:0002037 denotes cerebella
T5635 680-689 UBERON:0002037 denotes cerebella
T5636 726-729 CHEBI:16526 denotes CO2
T5637 734-748 CL:0000120 denotes granule neuron
T5638 836-842 NCBITaxon:27592 denotes bovine
T5639 843-848 UBERON:0001977 denotes serum
T5640 890-893 CHEBI:32588 denotes KCl
T5641 953-963 NCBITaxon:11632 denotes retrovirus
T5642 964-975 GO:0009293 denotes transducing
T5643 1020-1027 CHEBI:59132 denotes antigen
T5644 1044-1052 CHEBI:7507 denotes neomycin
T5645 1064-1072 SO:0005853 denotes cassette
T5646 1275-1279 CHEBI:42768 denotes G418
T5647 1534-1536 UBERON:0002037 denotes Cb
T5648 1536-1540 PR:000005591 denotes Cln3
T5649 1553-1555 UBERON:0002037 denotes Cb
T5650 1662-1665 CHEBI:16526 denotes CO2
T5651 1769-1777 CL:0000540 denotes Neuronal
T5652 1846-1854 CHEBI:60004 denotes cocktail
T5653 1865-1870 PR:000007479 denotes α-FGF
T5654 1879-1883 CHEBI:48518 denotes IBMX
T5655 1892-1895 CHEBI:37537 denotes TPA
T5656 1903-1912 CHEBI:42471 denotes forskolin
T5657 1919-1927 CHEBI:18243 denotes dopamine

craft-sa-dev

Id Subject Object Predicate Lexical cue
T5725 0-8 NN denotes Cln3Δex7
T5726 20-24 NNS denotes mice
T5727 8-9 HYPH denotes /
T5728 9-10 CD denotes 8
T5729 11-16 VB denotes knock
T5730 16-17 HYPH denotes -
T5731 17-19 RP denotes in
T5732 46-55 VBN denotes described
T5733 25-29 VBP denotes have
T5734 30-34 VBN denotes been
T5735 35-45 RB denotes previously
T5736 56-57 -LRB- denotes [
T5737 57-59 CD denotes 12
T5738 59-60 -RRB- denotes ]
T5739 60-61 . denotes .
T5740 61-241 sentence denotes Littermate pups from heterozygote crosses were used for primary culture establishment, by previously described methods that yield cerebellar granule neuron-enriched cultures [26].
T5741 62-72 NN denotes Littermate
T5742 73-77 NNS denotes pups
T5743 109-113 VBN denotes used
T5744 78-82 IN denotes from
T5745 83-95 NN denotes heterozygote
T5746 96-103 NNS denotes crosses
T5747 104-108 VBD denotes were
T5748 114-117 IN denotes for
T5749 118-125 JJ denotes primary
T5750 134-147 NN denotes establishment
T5751 126-133 NN denotes culture
T5752 147-149 , denotes ,
T5753 149-151 IN denotes by
T5754 152-162 RB denotes previously
T5755 163-172 VBN denotes described
T5756 173-180 NNS denotes methods
T5757 181-185 WDT denotes that
T5758 186-191 VBP denotes yield
T5759 192-202 JJ denotes cerebellar
T5760 211-217 NN denotes neuron
T5761 203-210 NN denotes granule
T5762 218-226 VBN denotes enriched
T5763 217-218 HYPH denotes -
T5764 227-235 NNS denotes cultures
T5765 236-237 -LRB- denotes [
T5766 237-239 CD denotes 26
T5767 239-240 -RRB- denotes ]
T5768 240-241 . denotes .
T5769 241-368 sentence denotes Postnatal day 4 (P4) cerebella were dissected in Hank's Balanced Salt Solution (HBSS, Sigma), supplemented with 35 mM glucose.
T5770 242-251 JJ denotes Postnatal
T5771 252-255 NN denotes day
T5772 263-272 NNS denotes cerebella
T5773 256-257 CD denotes 4
T5774 258-259 -LRB- denotes (
T5775 259-261 NN denotes P4
T5776 261-262 -RRB- denotes )
T5777 278-287 VBN denotes dissected
T5778 273-277 VBD denotes were
T5779 288-290 IN denotes in
T5780 291-295 NNP denotes Hank
T5781 312-320 NNP denotes Solution
T5782 295-297 POS denotes 's
T5783 298-306 NNP denotes Balanced
T5784 307-311 NNP denotes Salt
T5785 321-322 -LRB- denotes (
T5786 322-326 NN denotes HBSS
T5787 326-328 , denotes ,
T5788 328-333 NNP denotes Sigma
T5789 333-334 -RRB- denotes )
T5790 334-336 , denotes ,
T5791 336-348 VBN denotes supplemented
T5792 349-353 IN denotes with
T5793 354-356 CD denotes 35
T5794 357-359 NN denotes mM
T5795 360-367 NN denotes glucose
T5796 367-368 . denotes .
T5797 368-452 sentence denotes Tail biopsies were also collected for genomic DNA isolation and genotypic analysis.
T5798 369-373 NN denotes Tail
T5799 374-382 NNS denotes biopsies
T5800 393-402 VBN denotes collected
T5801 383-387 VBD denotes were
T5802 388-392 RB denotes also
T5803 403-406 IN denotes for
T5804 407-414 JJ denotes genomic
T5805 419-428 NN denotes isolation
T5806 415-418 NN denotes DNA
T5807 429-432 CC denotes and
T5808 433-442 JJ denotes genotypic
T5809 443-451 NN denotes analysis
T5810 451-452 . denotes .
T5811 452-646 sentence denotes Trypsin/EDTA (10 mg/ml, Sigma) and DNase I (100 μg/ml, Sigma), suspended in HBSS, helped dissociate cerebella for primary culture plating onto 0.01% poly-ornithine (Sigma) coated 100 mm dishes.
T5812 453-460 NN denotes Trypsin
T5813 461-465 NN denotes EDTA
T5814 460-461 HYPH denotes /
T5815 535-541 VBD denotes helped
T5816 466-467 -LRB- denotes (
T5817 477-482 NNP denotes Sigma
T5818 467-469 CD denotes 10
T5819 470-472 NN denotes mg
T5820 472-473 SYM denotes /
T5821 473-475 NN denotes ml
T5822 475-477 , denotes ,
T5823 482-483 -RRB- denotes )
T5824 484-487 CC denotes and
T5825 488-493 NN denotes DNase
T5826 494-495 CD denotes I
T5827 496-497 -LRB- denotes (
T5828 508-513 NNP denotes Sigma
T5829 497-500 CD denotes 100
T5830 501-503 NN denotes μg
T5831 503-504 SYM denotes /
T5832 504-506 NN denotes ml
T5833 506-508 , denotes ,
T5834 513-514 -RRB- denotes )
T5835 514-516 , denotes ,
T5836 516-525 VBN denotes suspended
T5837 526-528 IN denotes in
T5838 529-533 NN denotes HBSS
T5839 533-535 , denotes ,
T5840 542-552 VB denotes dissociate
T5841 553-562 NNS denotes cerebella
T5842 563-566 IN denotes for
T5843 567-574 JJ denotes primary
T5844 575-582 NN denotes culture
T5845 583-590 NN denotes plating
T5846 591-595 IN denotes onto
T5847 596-600 CD denotes 0.01
T5848 600-601 NN denotes %
T5849 602-616 NN denotes poly-ornithine
T5850 625-631 VBN denotes coated
T5851 617-618 -LRB- denotes (
T5852 618-623 NNP denotes Sigma
T5853 623-624 -RRB- denotes )
T5854 639-645 NNS denotes dishes
T5855 632-635 CD denotes 100
T5856 636-638 NN denotes mm
T5857 645-646 . denotes .
T5858 646-895 sentence denotes Primary cultures from individual cerebella were cultured overnight at 37°C, 5% CO2, in granule neuron growth media (Dulbecco's Modified Eagle Medium [DMEM, Gibco BRL #11995-065], 10% fetal bovine serum [Sigma #F-2442], supplemented with 24 mM KCl).
T5859 647-654 JJ denotes Primary
T5860 655-663 NNS denotes cultures
T5861 695-703 VBN denotes cultured
T5862 664-668 IN denotes from
T5863 669-679 JJ denotes individual
T5864 680-689 NNS denotes cerebella
T5865 690-694 VBD denotes were
T5866 704-713 RB denotes overnight
T5867 714-716 IN denotes at
T5868 717-719 CD denotes 37
T5869 719-721 NN denotes °C
T5870 721-723 , denotes ,
T5871 723-724 CD denotes 5
T5872 724-725 NN denotes %
T5873 726-729 NN denotes CO2
T5874 729-731 , denotes ,
T5875 731-733 IN denotes in
T5876 734-741 NN denotes granule
T5877 742-748 NN denotes neuron
T5878 756-761 NNS denotes media
T5879 749-755 NN denotes growth
T5880 762-763 -LRB- denotes (
T5881 763-771 NNP denotes Dulbecco
T5882 789-795 NNP denotes Medium
T5883 771-773 POS denotes 's
T5884 774-782 NNP denotes Modified
T5885 783-788 NNP denotes Eagle
T5886 796-797 -LRB- denotes [
T5887 797-801 NN denotes DMEM
T5888 801-803 , denotes ,
T5889 803-808 NNP denotes Gibco
T5890 813-814 NN denotes #
T5891 809-812 NNP denotes BRL
T5892 814-819 CD denotes 11995
T5893 819-820 HYPH denotes -
T5894 820-823 CD denotes 065
T5895 823-824 -RRB- denotes ]
T5896 824-826 , denotes ,
T5897 826-828 CD denotes 10
T5898 828-829 NN denotes %
T5899 830-835 JJ denotes fetal
T5900 843-848 NN denotes serum
T5901 836-842 JJ denotes bovine
T5902 849-850 -LRB- denotes [
T5903 850-855 NNP denotes Sigma
T5904 857-858 NN denotes F
T5905 856-857 NN denotes #
T5906 858-859 HYPH denotes -
T5907 859-863 CD denotes 2442
T5908 863-864 -RRB- denotes ]
T5909 864-866 , denotes ,
T5910 866-878 VBN denotes supplemented
T5911 879-883 IN denotes with
T5912 884-886 CD denotes 24
T5913 887-889 NN denotes mM
T5914 890-893 NN denotes KCl
T5915 893-894 -RRB- denotes )
T5916 894-895 . denotes .
T5917 895-1108 sentence denotes Infection was performed the following day with defective retrovirus transducing the temperature-sensitive tsA58/U19 large T antigen and a selection neomycin-resistance cassette [27], as previously described [28].
T5918 896-905 NN denotes Infection
T5919 910-919 VBN denotes performed
T5920 906-909 VBD denotes was
T5921 920-923 DT denotes the
T5922 934-937 NN denotes day
T5923 924-933 JJ denotes following
T5924 938-942 IN denotes with
T5925 943-952 JJ denotes defective
T5926 953-963 NN denotes retrovirus
T5927 964-975 VBG denotes transducing
T5928 976-979 DT denotes the
T5929 1020-1027 NN denotes antigen
T5930 980-991 NN denotes temperature
T5931 992-1001 JJ denotes sensitive
T5932 991-992 HYPH denotes -
T5933 1002-1007 NN denotes tsA58
T5934 1008-1011 NN denotes U19
T5935 1007-1008 HYPH denotes /
T5936 1012-1017 JJ denotes large
T5937 1018-1019 NN denotes T
T5938 1028-1031 CC denotes and
T5939 1032-1033 DT denotes a
T5940 1064-1072 NN denotes cassette
T5941 1034-1043 NN denotes selection
T5942 1044-1052 NN denotes neomycin
T5943 1053-1063 NN denotes resistance
T5944 1052-1053 HYPH denotes -
T5945 1073-1074 -LRB- denotes [
T5946 1074-1076 CD denotes 27
T5947 1076-1077 -RRB- denotes ]
T5948 1077-1079 , denotes ,
T5949 1079-1081 IN denotes as
T5950 1093-1102 VBN denotes described
T5951 1082-1092 RB denotes previously
T5952 1103-1104 -LRB- denotes [
T5953 1104-1106 CD denotes 28
T5954 1106-1107 -RRB- denotes ]
T5955 1107-1108 . denotes .
T5956 1108-1280 sentence denotes Following infection, cultures were shifted to the tsA58/U19 permissive growth temperature of 33°C and selection was in the same growth media as above, with 200 μg/ml G418.
T5957 1109-1118 VBG denotes Following
T5958 1144-1151 VBN denotes shifted
T5959 1119-1128 NN denotes infection
T5960 1128-1130 , denotes ,
T5961 1130-1138 NNS denotes cultures
T5962 1139-1143 VBD denotes were
T5963 1152-1154 IN denotes to
T5964 1155-1158 DT denotes the
T5965 1187-1198 NN denotes temperature
T5966 1159-1164 NN denotes tsA58
T5967 1165-1168 NN denotes U19
T5968 1164-1165 HYPH denotes /
T5969 1169-1179 JJ denotes permissive
T5970 1180-1186 NN denotes growth
T5971 1199-1201 IN denotes of
T5972 1202-1204 CD denotes 33
T5973 1204-1206 NN denotes °C
T5974 1207-1210 CC denotes and
T5975 1211-1220 NN denotes selection
T5976 1221-1224 VBD denotes was
T5977 1225-1227 IN denotes in
T5978 1228-1231 DT denotes the
T5979 1244-1249 NNS denotes media
T5980 1232-1236 JJ denotes same
T5981 1237-1243 NN denotes growth
T5982 1250-1252 IN denotes as
T5983 1253-1258 RB denotes above
T5984 1258-1260 , denotes ,
T5985 1260-1264 IN denotes with
T5986 1265-1268 CD denotes 200
T5987 1269-1271 NN denotes μg
T5988 1275-1279 NN denotes G418
T5989 1271-1272 SYM denotes /
T5990 1272-1274 NN denotes ml
T5991 1279-1280 . denotes .
T5992 1280-1419 sentence denotes Conditionally immortalized colonies were isolated 3–9 weeks post-infection and expanded for frozen stocks and further sub-clone isolation.
T5993 1281-1294 RB denotes Conditionally
T5994 1295-1307 VBN denotes immortalized
T5995 1308-1316 NNS denotes colonies
T5996 1322-1330 VBN denotes isolated
T5997 1317-1321 VBD denotes were
T5998 1331-1332 CD denotes 3
T5999 1333-1334 CD denotes 9
T6000 1332-1333 SYM denotes –
T6001 1335-1340 NNS denotes weeks
T6002 1341-1355 RB denotes post-infection
T6003 1356-1359 CC denotes and
T6004 1360-1368 VBN denotes expanded
T6005 1369-1372 IN denotes for
T6006 1373-1379 JJ denotes frozen
T6007 1380-1386 NNS denotes stocks
T6008 1387-1390 CC denotes and
T6009 1391-1398 JJ denotes further
T6010 1409-1418 NN denotes isolation
T6011 1399-1408 NN denotes sub-clone
T6012 1418-1419 . denotes .
T6013 1419-1552 sentence denotes Multiple clonal lines were obtained for each genotype and all phenotypes were confirmed in at least 2 independent CbCln3 cell lines.
T6014 1420-1428 JJ denotes Multiple
T6015 1436-1441 NNS denotes lines
T6016 1429-1435 JJ denotes clonal
T6017 1447-1455 VBN denotes obtained
T6018 1442-1446 VBD denotes were
T6019 1456-1459 IN denotes for
T6020 1460-1464 DT denotes each
T6021 1465-1473 NN denotes genotype
T6022 1474-1477 CC denotes and
T6023 1478-1481 DT denotes all
T6024 1482-1492 NNS denotes phenotypes
T6025 1493-1497 VBD denotes were
T6026 1498-1507 VBN denotes confirmed
T6027 1508-1510 IN denotes in
T6028 1511-1513 RB denotes at
T6029 1520-1521 CD denotes 2
T6030 1514-1519 RBS denotes least
T6031 1546-1551 NNS denotes lines
T6032 1522-1533 JJ denotes independent
T6033 1534-1540 NN denotes CbCln3
T6034 1541-1545 NN denotes cell
T6035 1551-1552 . denotes .
T6036 1552-1677 sentence denotes CbCln3 cell lines were maintained on 0.01% poly-ornithine coated dishes at 30–90% confluency, in 33°C and 5% CO2 atmosphere.
T6037 1553-1559 NN denotes CbCln3
T6038 1565-1570 NNS denotes lines
T6039 1560-1564 NN denotes cell
T6040 1576-1586 VBN denotes maintained
T6041 1571-1575 VBD denotes were
T6042 1587-1589 IN denotes on
T6043 1590-1594 CD denotes 0.01
T6044 1594-1595 NN denotes %
T6045 1618-1624 NNS denotes dishes
T6046 1596-1600 NN denotes poly
T6047 1601-1610 NN denotes ornithine
T6048 1600-1601 HYPH denotes -
T6049 1611-1617 VBN denotes coated
T6050 1625-1627 IN denotes at
T6051 1628-1630 CD denotes 30
T6052 1631-1633 CD denotes 90
T6053 1630-1631 SYM denotes –
T6054 1633-1634 NN denotes %
T6055 1635-1645 NN denotes confluency
T6056 1645-1647 , denotes ,
T6057 1647-1649 IN denotes in
T6058 1650-1652 CD denotes 33
T6059 1652-1654 NN denotes °C
T6060 1655-1658 CC denotes and
T6061 1659-1660 CD denotes 5
T6062 1666-1676 NN denotes atmosphere
T6063 1660-1661 SYM denotes %
T6064 1662-1665 NN denotes CO2
T6065 1676-1677 . denotes .
T6066 1677-1768 sentence denotes Passage number was recorded (up to ~20 passages), but had no apparent impact on phenotype.
T6067 1678-1685 NN denotes Passage
T6068 1686-1692 NN denotes number
T6069 1697-1705 VBN denotes recorded
T6070 1693-1696 VBD denotes was
T6071 1706-1707 -LRB- denotes (
T6072 1717-1725 NNS denotes passages
T6073 1707-1709 IN denotes up
T6074 1714-1716 CD denotes 20
T6075 1710-1712 IN denotes to
T6076 1713-1714 SYM denotes ~
T6077 1725-1726 -RRB- denotes )
T6078 1726-1728 , denotes ,
T6079 1728-1731 CC denotes but
T6080 1732-1735 VBD denotes had
T6081 1736-1738 DT denotes no
T6082 1748-1754 NN denotes impact
T6083 1739-1747 JJ denotes apparent
T6084 1755-1757 IN denotes on
T6085 1758-1767 NN denotes phenotype
T6086 1767-1768 . denotes .
T6087 1768-1936 sentence denotes Neuronal differentiation was as previously described [29] with the following cocktail: 10 ng/ml α-FGF, 250 μM IBMX, 200 nM TPA, 50 μM forskolin, 5 μM dopamine (Sigma).
T6088 1769-1777 JJ denotes Neuronal
T6089 1778-1793 NN denotes differentiation
T6090 1794-1797 VBD denotes was
T6091 1798-1800 IN denotes as
T6092 1812-1821 VBN denotes described
T6093 1801-1811 RB denotes previously
T6094 1822-1823 -LRB- denotes [
T6095 1823-1825 CD denotes 29
T6096 1825-1826 -RRB- denotes ]
T6097 1827-1831 IN denotes with
T6098 1832-1835 DT denotes the
T6099 1846-1854 NN denotes cocktail
T6100 1836-1845 JJ denotes following
T6101 1854-1856 : denotes :
T6102 1856-1858 CD denotes 10
T6103 1859-1861 NN denotes ng
T6104 1867-1870 NN denotes FGF
T6105 1861-1862 SYM denotes /
T6106 1862-1864 NN denotes ml
T6107 1865-1866 NN denotes α
T6108 1866-1867 HYPH denotes -
T6109 1870-1872 , denotes ,
T6110 1872-1875 CD denotes 250
T6111 1876-1878 NN denotes μM
T6112 1879-1883 NN denotes IBMX
T6113 1883-1885 , denotes ,
T6114 1885-1888 CD denotes 200
T6115 1889-1891 NN denotes nM
T6116 1892-1895 NN denotes TPA
T6117 1895-1897 , denotes ,
T6118 1897-1899 CD denotes 50
T6119 1900-1902 NN denotes μM
T6120 1903-1912 NN denotes forskolin
T6121 1912-1914 , denotes ,
T6122 1914-1915 CD denotes 5
T6123 1916-1918 NN denotes μM
T6124 1919-1927 NN denotes dopamine
T6125 1928-1929 -LRB- denotes (
T6126 1929-1934 NNP denotes Sigma
T6127 1934-1935 -RRB- denotes )
T6128 1935-1936 . denotes .
R3342 T5725 T5726 nmod Cln3Δex7,mice
R3343 T5726 T5732 nsubjpass mice,described
R3344 T5727 T5725 punct /,Cln3Δex7
R3345 T5728 T5725 nummod 8,Cln3Δex7
R3346 T5729 T5726 amod knock,mice
R3347 T5730 T5729 punct -,knock
R3348 T5731 T5729 prt in,knock
R3349 T5733 T5732 aux have,described
R3350 T5734 T5732 auxpass been,described
R3351 T5735 T5732 advmod previously,described
R3352 T5736 T5737 punct [,12
R3353 T5737 T5732 parataxis 12,described
R3354 T5738 T5737 punct ],12
R3355 T5739 T5732 punct .,described
R3356 T5741 T5742 compound Littermate,pups
R3357 T5742 T5743 nsubjpass pups,used
R3358 T5744 T5742 prep from,pups
R3359 T5745 T5746 compound heterozygote,crosses
R3360 T5746 T5744 pobj crosses,from
R3361 T5747 T5743 auxpass were,used
R3362 T5748 T5743 prep for,used
R3363 T5749 T5750 amod primary,establishment
R3364 T5750 T5748 pobj establishment,for
R3365 T5751 T5750 compound culture,establishment
R3366 T5752 T5743 punct ", ",used
R3367 T5753 T5743 prep by,used
R3368 T5754 T5755 advmod previously,described
R3369 T5755 T5756 amod described,methods
R3370 T5756 T5753 pobj methods,by
R3371 T5757 T5758 dep that,yield
R3372 T5758 T5756 relcl yield,methods
R3373 T5759 T5760 amod cerebellar,neuron
R3374 T5760 T5762 npadvmod neuron,enriched
R3375 T5761 T5760 compound granule,neuron
R3376 T5762 T5764 amod enriched,cultures
R3377 T5763 T5762 punct -,enriched
R3378 T5764 T5758 dobj cultures,yield
R3379 T5765 T5766 punct [,26
R3380 T5766 T5758 parataxis 26,yield
R3381 T5767 T5766 punct ],26
R3382 T5768 T5743 punct .,used
R3383 T5770 T5771 amod Postnatal,day
R3384 T5771 T5772 nmod day,cerebella
R3385 T5772 T5777 nsubjpass cerebella,dissected
R3386 T5773 T5771 nummod 4,day
R3387 T5774 T5771 punct (,day
R3388 T5775 T5771 appos P4,day
R3389 T5776 T5772 punct ),cerebella
R3390 T5778 T5777 auxpass were,dissected
R3391 T5779 T5777 prep in,dissected
R3392 T5780 T5781 poss Hank,Solution
R3393 T5781 T5779 pobj Solution,in
R3394 T5782 T5780 case 's,Hank
R3395 T5783 T5781 compound Balanced,Solution
R3396 T5784 T5781 compound Salt,Solution
R3397 T5785 T5781 punct (,Solution
R3398 T5786 T5781 appos HBSS,Solution
R3399 T5787 T5788 punct ", ",Sigma
R3400 T5788 T5781 parataxis Sigma,Solution
R3401 T5789 T5788 punct ),Sigma
R3402 T5790 T5781 punct ", ",Solution
R3403 T5791 T5781 acl supplemented,Solution
R3404 T5792 T5791 prep with,supplemented
R3405 T5793 T5794 nummod 35,mM
R3406 T5794 T5795 compound mM,glucose
R3407 T5795 T5792 pobj glucose,with
R3408 T5796 T5777 punct .,dissected
R3409 T5798 T5799 compound Tail,biopsies
R3410 T5799 T5800 nsubjpass biopsies,collected
R3411 T5801 T5800 auxpass were,collected
R3412 T5802 T5800 advmod also,collected
R3413 T5803 T5800 prep for,collected
R3414 T5804 T5805 amod genomic,isolation
R3415 T5805 T5803 pobj isolation,for
R3416 T5806 T5805 compound DNA,isolation
R3417 T5807 T5805 cc and,isolation
R3418 T5808 T5809 amod genotypic,analysis
R3419 T5809 T5805 conj analysis,isolation
R3420 T5810 T5800 punct .,collected
R3421 T5812 T5813 compound Trypsin,EDTA
R3422 T5813 T5815 nsubj EDTA,helped
R3423 T5814 T5813 punct /,EDTA
R3424 T5816 T5817 punct (,Sigma
R3425 T5817 T5813 parataxis Sigma,EDTA
R3426 T5818 T5819 nummod 10,mg
R3427 T5819 T5817 dep mg,Sigma
R3428 T5820 T5821 punct /,ml
R3429 T5821 T5819 prep ml,mg
R3430 T5822 T5817 punct ", ",Sigma
R3431 T5823 T5817 punct ),Sigma
R3432 T5824 T5813 cc and,EDTA
R3433 T5825 T5813 conj DNase,EDTA
R3434 T5826 T5825 nummod I,DNase
R3435 T5827 T5828 punct (,Sigma
R3436 T5828 T5825 parataxis Sigma,DNase
R3437 T5829 T5830 nummod 100,μg
R3438 T5830 T5828 dep μg,Sigma
R3439 T5831 T5832 punct /,ml
R3440 T5832 T5830 prep ml,μg
R3441 T5833 T5828 punct ", ",Sigma
R3442 T5834 T5828 punct ),Sigma
R3443 T5835 T5813 punct ", ",EDTA
R3444 T5836 T5813 acl suspended,EDTA
R3445 T5837 T5836 prep in,suspended
R3446 T5838 T5837 pobj HBSS,in
R3447 T5839 T5815 punct ", ",helped
R3448 T5840 T5815 xcomp dissociate,helped
R3449 T5841 T5840 dobj cerebella,dissociate
R3450 T5842 T5840 prep for,dissociate
R3451 T5843 T5844 amod primary,culture
R3452 T5844 T5845 compound culture,plating
R3453 T5845 T5842 pobj plating,for
R3454 T5846 T5845 prep onto,plating
R3455 T5847 T5848 nummod 0.01,%
R3456 T5848 T5849 compound %,poly-ornithine
R3457 T5849 T5850 npadvmod poly-ornithine,coated
R3458 T5850 T5854 amod coated,dishes
R3459 T5851 T5852 punct (,Sigma
R3460 T5852 T5849 parataxis Sigma,poly-ornithine
R3461 T5853 T5852 punct ),Sigma
R3462 T5854 T5846 pobj dishes,onto
R3463 T5855 T5856 nummod 100,mm
R3464 T5856 T5854 compound mm,dishes
R3465 T5857 T5815 punct .,helped
R3466 T5859 T5860 amod Primary,cultures
R3467 T5860 T5861 nsubjpass cultures,cultured
R3468 T5862 T5860 prep from,cultures
R3469 T5863 T5864 amod individual,cerebella
R3470 T5864 T5862 pobj cerebella,from
R3471 T5865 T5861 auxpass were,cultured
R3472 T5866 T5861 advmod overnight,cultured
R3473 T5867 T5861 prep at,cultured
R3474 T5868 T5869 nummod 37,°C
R3475 T5869 T5867 pobj °C,at
R3476 T5870 T5869 punct ", ",°C
R3477 T5871 T5872 nummod 5,%
R3478 T5872 T5873 compound %,CO2
R3479 T5873 T5869 appos CO2,°C
R3480 T5874 T5861 punct ", ",cultured
R3481 T5875 T5861 prep in,cultured
R3482 T5876 T5877 compound granule,neuron
R3483 T5877 T5878 compound neuron,media
R3484 T5878 T5875 pobj media,in
R3485 T5879 T5878 compound growth,media
R3486 T5880 T5878 punct (,media
R3487 T5881 T5882 poss Dulbecco,Medium
R3488 T5882 T5878 appos Medium,media
R3489 T5883 T5881 case 's,Dulbecco
R3490 T5884 T5882 compound Modified,Medium
R3491 T5885 T5882 compound Eagle,Medium
R3492 T5886 T5882 punct [,Medium
R3493 T5887 T5882 appos DMEM,Medium
R3494 T5888 T5882 punct ", ",Medium
R3495 T5889 T5890 compound Gibco,#
R3496 T5890 T5882 appos #,Medium
R3497 T5891 T5890 compound BRL,#
R3498 T5892 T5890 nummod 11995,#
R3499 T5893 T5890 punct -,#
R3500 T5894 T5890 nummod 065,#
R3501 T5895 T5882 punct ],Medium
R3502 T5896 T5882 punct ", ",Medium
R3503 T5897 T5898 nummod 10,%
R3504 T5898 T5882 appos %,Medium
R3505 T5899 T5900 amod fetal,serum
R3506 T5900 T5898 appos serum,%
R3507 T5901 T5900 amod bovine,serum
R3508 T5902 T5900 punct [,serum
R3509 T5903 T5904 compound Sigma,F
R3510 T5904 T5900 appos F,serum
R3511 T5905 T5904 compound #,F
R3512 T5906 T5904 punct -,F
R3513 T5907 T5904 nummod 2442,F
R3514 T5908 T5882 punct ],Medium
R3515 T5909 T5882 punct ", ",Medium
R3516 T5910 T5882 acl supplemented,Medium
R3517 T5911 T5910 prep with,supplemented
R3518 T5912 T5913 nummod 24,mM
R3519 T5913 T5914 compound mM,KCl
R3520 T5914 T5911 pobj KCl,with
R3521 T5915 T5861 punct ),cultured
R3522 T5916 T5861 punct .,cultured
R3523 T5918 T5919 nsubjpass Infection,performed
R3524 T5920 T5919 auxpass was,performed
R3525 T5921 T5922 det the,day
R3526 T5922 T5919 npadvmod day,performed
R3527 T5923 T5922 amod following,day
R3528 T5924 T5919 prep with,performed
R3529 T5925 T5926 amod defective,retrovirus
R3530 T5926 T5927 nsubj retrovirus,transducing
R3531 T5927 T5924 pcomp transducing,with
R3532 T5928 T5929 det the,antigen
R3533 T5929 T5927 dobj antigen,transducing
R3534 T5930 T5931 npadvmod temperature,sensitive
R3535 T5931 T5929 amod sensitive,antigen
R3536 T5932 T5931 punct -,sensitive
R3537 T5933 T5934 nmod tsA58,U19
R3538 T5934 T5929 nmod U19,antigen
R3539 T5935 T5934 punct /,U19
R3540 T5936 T5929 amod large,antigen
R3541 T5937 T5929 compound T,antigen
R3542 T5938 T5929 cc and,antigen
R3543 T5939 T5940 det a,cassette
R3544 T5940 T5929 conj cassette,antigen
R3545 T5941 T5940 compound selection,cassette
R3546 T5942 T5943 compound neomycin,resistance
R3547 T5943 T5940 compound resistance,cassette
R3548 T5944 T5943 punct -,resistance
R3549 T5945 T5946 punct [,27
R3550 T5946 T5940 parataxis 27,cassette
R3551 T5947 T5946 punct ],27
R3552 T5948 T5927 punct ", ",transducing
R3553 T5949 T5950 mark as,described
R3554 T5950 T5927 advcl described,transducing
R3555 T5951 T5950 advmod previously,described
R3556 T5952 T5953 punct [,28
R3557 T5953 T5950 parataxis 28,described
R3558 T5954 T5953 punct ],28
R3559 T5955 T5919 punct .,performed
R3560 T5957 T5958 prep Following,shifted
R3561 T5959 T5957 pobj infection,Following
R3562 T5960 T5958 punct ", ",shifted
R3563 T5961 T5958 nsubjpass cultures,shifted
R3564 T5962 T5958 auxpass were,shifted
R3565 T5963 T5958 prep to,shifted
R3566 T5964 T5965 det the,temperature
R3567 T5965 T5963 pobj temperature,to
R3568 T5966 T5967 compound tsA58,U19
R3569 T5967 T5969 npadvmod U19,permissive
R3570 T5968 T5967 punct /,U19
R3571 T5969 T5965 amod permissive,temperature
R3572 T5970 T5965 compound growth,temperature
R3573 T5971 T5965 prep of,temperature
R3574 T5972 T5973 nummod 33,°C
R3575 T5973 T5971 pobj °C,of
R3576 T5974 T5958 cc and,shifted
R3577 T5975 T5976 nsubj selection,was
R3578 T5976 T5958 conj was,shifted
R3579 T5977 T5976 prep in,was
R3580 T5978 T5979 det the,media
R3581 T5979 T5977 pobj media,in
R3582 T5980 T5979 amod same,media
R3583 T5981 T5979 compound growth,media
R3584 T5982 T5979 prep as,media
R3585 T5983 T5982 pcomp above,as
R3586 T5984 T5976 punct ", ",was
R3587 T5985 T5976 prep with,was
R3588 T5986 T5987 nummod 200,μg
R3589 T5987 T5988 nmod μg,G418
R3590 T5988 T5985 pobj G418,with
R3591 T5989 T5990 punct /,ml
R3592 T5990 T5987 prep ml,μg
R3593 T5991 T5976 punct .,was
R3594 T5993 T5994 advmod Conditionally,immortalized
R3595 T5994 T5995 amod immortalized,colonies
R3596 T5995 T5996 nsubjpass colonies,isolated
R3597 T5997 T5996 auxpass were,isolated
R3598 T5998 T5999 quantmod 3,9
R3599 T5999 T6001 nummod 9,weeks
R3600 T6000 T5999 punct –,9
R3601 T6001 T6002 npadvmod weeks,post-infection
R3602 T6002 T5996 advmod post-infection,isolated
R3603 T6003 T6004 cc and,expanded
R3604 T6004 T5996 dep expanded,isolated
R3605 T6005 T6004 prep for,expanded
R3606 T6006 T6007 amod frozen,stocks
R3607 T6007 T6005 pobj stocks,for
R3608 T6008 T6007 cc and,stocks
R3609 T6009 T6010 amod further,isolation
R3610 T6010 T6007 conj isolation,stocks
R3611 T6011 T6010 compound sub-clone,isolation
R3612 T6012 T5996 punct .,isolated
R3613 T6014 T6015 amod Multiple,lines
R3614 T6015 T6017 nsubjpass lines,obtained
R3615 T6016 T6015 amod clonal,lines
R3616 T6018 T6017 auxpass were,obtained
R3617 T6019 T6017 prep for,obtained
R3618 T6020 T6021 det each,genotype
R3619 T6021 T6019 pobj genotype,for
R3620 T6022 T6021 cc and,genotype
R3621 T6023 T6024 det all,phenotypes
R3622 T6024 T6021 conj phenotypes,genotype
R3623 T6025 T6026 auxpass were,confirmed
R3624 T6026 T6017 dep confirmed,obtained
R3625 T6027 T6026 prep in,confirmed
R3626 T6028 T6029 advmod at,2
R3627 T6029 T6031 nummod 2,lines
R3628 T6030 T6029 advmod least,2
R3629 T6031 T6027 pobj lines,in
R3630 T6032 T6031 amod independent,lines
R3631 T6033 T6031 compound CbCln3,lines
R3632 T6034 T6031 compound cell,lines
R3633 T6035 T6017 punct .,obtained
R3634 T6037 T6038 compound CbCln3,lines
R3635 T6038 T6040 nsubjpass lines,maintained
R3636 T6039 T6038 compound cell,lines
R3637 T6041 T6040 auxpass were,maintained
R3638 T6042 T6040 prep on,maintained
R3639 T6043 T6044 nummod 0.01,%
R3640 T6044 T6045 nmod %,dishes
R3641 T6045 T6042 pobj dishes,on
R3642 T6046 T6047 compound poly,ornithine
R3643 T6047 T6049 npadvmod ornithine,coated
R3644 T6048 T6047 punct -,ornithine
R3645 T6049 T6045 amod coated,dishes
R3646 T6050 T6040 prep at,maintained
R3647 T6051 T6052 quantmod 30,90
R3648 T6052 T6054 nummod 90,%
R3649 T6053 T6052 punct –,90
R3650 T6054 T6055 compound %,confluency
R3651 T6055 T6050 pobj confluency,at
R3652 T6056 T6040 punct ", ",maintained
R3653 T6057 T6040 prep in,maintained
R3654 T6058 T6059 nummod 33,°C
R3655 T6059 T6057 pobj °C,in
R3656 T6060 T6059 cc and,°C
R3657 T6061 T6062 nummod 5,atmosphere
R3658 T6062 T6059 conj atmosphere,°C
R3659 T6063 T6061 punct %,5
R3660 T6064 T6062 compound CO2,atmosphere
R3661 T6065 T6040 punct .,maintained
R3662 T6067 T6068 compound Passage,number
R3663 T6068 T6069 nsubjpass number,recorded
R3664 T6070 T6069 auxpass was,recorded
R3665 T6071 T6072 punct (,passages
R3666 T6072 T6069 parataxis passages,recorded
R3667 T6073 T6074 quantmod up,20
R3668 T6074 T6072 nummod 20,passages
R3669 T6075 T6074 quantmod to,20
R3670 T6076 T6074 punct ~,20
R3671 T6077 T6072 punct ),passages
R3672 T6078 T6069 punct ", ",recorded
R3673 T6079 T6069 cc but,recorded
R3674 T6080 T6069 conj had,recorded
R3675 T6081 T6082 det no,impact
R3676 T6082 T6080 dobj impact,had
R3677 T6083 T6082 amod apparent,impact
R3678 T6084 T6082 prep on,impact
R3679 T6085 T6084 pobj phenotype,on
R3680 T6086 T6069 punct .,recorded
R3681 T6088 T6089 amod Neuronal,differentiation
R3682 T6089 T6090 nsubj differentiation,was
R3683 T6091 T6092 mark as,described
R3684 T6092 T6090 advcl described,was
R3685 T6093 T6092 advmod previously,described
R3686 T6094 T6095 punct [,29
R3687 T6095 T6092 parataxis 29,described
R3688 T6096 T6095 punct ],29
R3689 T6097 T6090 prep with,was
R3690 T6098 T6099 det the,cocktail
R3691 T6099 T6097 pobj cocktail,with
R3692 T6100 T6099 amod following,cocktail
R3693 T6101 T6099 punct : ,cocktail
R3694 T6102 T6103 nummod 10,ng
R3695 T6103 T6104 nmod ng,FGF
R3696 T6104 T6099 appos FGF,cocktail
R3697 T6105 T6106 punct /,ml
R3698 T6106 T6103 prep ml,ng
R3699 T6107 T6104 compound α,FGF
R3700 T6108 T6104 punct -,FGF
R3701 T6109 T6104 punct ", ",FGF
R3702 T6110 T6111 nummod 250,μM
R3703 T6111 T6112 compound μM,IBMX
R3704 T6112 T6104 appos IBMX,FGF
R3705 T6113 T6104 punct ", ",FGF
R3706 T6114 T6115 nummod 200,nM
R3707 T6115 T6116 compound nM,TPA
R3708 T6116 T6104 appos TPA,FGF
R3709 T6117 T6104 punct ", ",FGF
R3710 T6118 T6119 nummod 50,μM
R3711 T6119 T6120 compound μM,forskolin
R3712 T6120 T6104 appos forskolin,FGF
R3713 T6121 T6104 punct ", ",FGF
R3714 T6122 T6123 nummod 5,μM
R3715 T6123 T6124 compound μM,dopamine
R3716 T6124 T6104 appos dopamine,FGF
R3717 T6125 T6126 punct (,Sigma
R3718 T6126 T6104 parataxis Sigma,FGF
R3719 T6127 T6126 punct ),Sigma
R3720 T6128 T6090 punct .,was

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T5663 0-4 PR_EXT:000005591 denotes Cln3
T5664 20-24 NCBITaxon:10088 denotes mice
T5665 73-77 NCBITaxon_UBERON_EXT:pup denotes pups
T5666 192-202 UBERON:0002037 denotes cerebellar
T5667 192-217 CL:0001031 denotes cerebellar granule neuron
T5668 246-251 GO:0007567 denotes natal
T5669 263-272 UBERON:0002037 denotes cerebella
T5670 307-320 CHEBI_EXT:saline_solution denotes Salt Solution
T5671 360-367 CHEBI:17234 denotes glucose
T5672 369-373 UBERON:0002415 denotes Tail
T5673 407-418 SO_EXT:genomic_DNA denotes genomic DNA
T5674 415-418 CHEBI_SO_EXT:DNA denotes DNA
T5675 433-442 SO_EXT:genotype_or_entity_with_genotype denotes genotypic
T5676 453-460 PR_EXT:000027795 denotes Trypsin
T5677 461-465 CHEBI_EXT:EDTA denotes EDTA
T5678 488-495 GO_PR_EXT:deoxyribonuclease_I denotes DNase I
T5679 553-562 UBERON:0002037 denotes cerebella
T5680 607-616 CHEBI_EXT:ornithine denotes ornithine
T5681 680-689 UBERON:0002037 denotes cerebella
T5682 726-729 CHEBI:16526 denotes CO2
T5683 734-748 CL:0000120 denotes granule neuron
T5684 749-755 GO_EXT:biological_growth_entity_or_process denotes growth
T5685 836-842 NCBITaxon:27592 denotes bovine
T5686 843-848 UBERON:0001977 denotes serum
T5687 890-893 CHEBI:32588 denotes KCl
T5688 953-963 NCBITaxon:11632 denotes retrovirus
T5689 964-975 GO:0009293 denotes transducing
T5690 1020-1027 CHEBI_EXT:59132 denotes antigen
T5691 1044-1052 CHEBI:7507 denotes neomycin
T5692 1064-1072 SO_EXT:0005853 denotes cassette
T5693 1180-1186 GO_EXT:biological_growth_entity_or_process denotes growth
T5694 1237-1243 GO_EXT:biological_growth_entity_or_process denotes growth
T5695 1275-1279 CHEBI:42768 denotes G418
T5696 1429-1435 SO_EXT:sequence_cloned_entity denotes clonal
T5697 1465-1473 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T5698 1534-1536 UBERON:0002037 denotes Cb
T5699 1536-1540 PR_EXT:000005591 denotes Cln3
T5700 1541-1545 CL_GO_EXT:cell denotes cell
T5701 1553-1555 UBERON:0002037 denotes Cb
T5702 1560-1564 CL_GO_EXT:cell denotes cell
T5703 1601-1610 CHEBI_EXT:ornithine denotes ornithine
T5704 1662-1665 CHEBI:16526 denotes CO2
T5705 1769-1777 CL:0000540 denotes Neuronal
T5706 1778-1793 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T5707 1846-1854 CHEBI:60004 denotes cocktail
T5708 1865-1870 PR_EXT:000007479 denotes α-FGF
T5709 1879-1883 CHEBI:48518 denotes IBMX
T5710 1892-1895 CHEBI:37537 denotes TPA
T5711 1903-1912 CHEBI:42471 denotes forskolin
T5712 1919-1927 CHEBI:18243 denotes dopamine

2_test

Id Subject Object Predicate Lexical cue
15588329-12374761-12950162 57-59 12374761 denotes 12
15588329-8248201-12950163 237-239 8248201 denotes 26
15588329-3023876-12950164 1074-1076 3023876 denotes 27
15588329-9671979-12950165 1104-1106 9671979 denotes 28
15588329-11092756-12950166 1823-1825 11092756 denotes 29