Id |
Subject |
Object |
Predicate |
Lexical cue |
T5720 |
84-89 |
NNS |
denotes |
lines |
T5713 |
0-10 |
NN |
denotes |
Generation |
T5714 |
10-12 |
, |
denotes |
, |
T5715 |
12-23 |
NN |
denotes |
maintenance |
T5716 |
24-27 |
CC |
denotes |
and |
T5717 |
28-43 |
NN |
denotes |
differentiation |
T5718 |
44-46 |
IN |
denotes |
of |
T5719 |
47-53 |
NN |
denotes |
CbCln3 |
T5721 |
54-64 |
JJ |
denotes |
cerebellar |
T5722 |
65-73 |
JJ |
denotes |
neuronal |
T5723 |
74-83 |
NN |
denotes |
precursor |
T5724 |
89-151 |
sentence |
denotes |
Cln3Δex7/8 knock-in mice have been previously described [12]. |
T5725 |
90-98 |
NN |
denotes |
Cln3Δex7 |
T5726 |
110-114 |
NNS |
denotes |
mice |
T5727 |
98-99 |
HYPH |
denotes |
/ |
T5728 |
99-100 |
CD |
denotes |
8 |
T5729 |
101-106 |
VB |
denotes |
knock |
T5730 |
106-107 |
HYPH |
denotes |
- |
T5731 |
107-109 |
RP |
denotes |
in |
T5732 |
136-145 |
VBN |
denotes |
described |
T5733 |
115-119 |
VBP |
denotes |
have |
T5734 |
120-124 |
VBN |
denotes |
been |
T5735 |
125-135 |
RB |
denotes |
previously |
T5736 |
146-147 |
-LRB- |
denotes |
[ |
T5737 |
147-149 |
CD |
denotes |
12 |
T5738 |
149-150 |
-RRB- |
denotes |
] |
T5739 |
150-151 |
. |
denotes |
. |
T5740 |
151-331 |
sentence |
denotes |
Littermate pups from heterozygote crosses were used for primary culture establishment, by previously described methods that yield cerebellar granule neuron-enriched cultures [26]. |
T5741 |
152-162 |
NN |
denotes |
Littermate |
T5742 |
163-167 |
NNS |
denotes |
pups |
T5743 |
199-203 |
VBN |
denotes |
used |
T5744 |
168-172 |
IN |
denotes |
from |
T5745 |
173-185 |
NN |
denotes |
heterozygote |
T5746 |
186-193 |
NNS |
denotes |
crosses |
T5747 |
194-198 |
VBD |
denotes |
were |
T5748 |
204-207 |
IN |
denotes |
for |
T5749 |
208-215 |
JJ |
denotes |
primary |
T5750 |
224-237 |
NN |
denotes |
establishment |
T5751 |
216-223 |
NN |
denotes |
culture |
T5752 |
237-239 |
, |
denotes |
, |
T5753 |
239-241 |
IN |
denotes |
by |
T5754 |
242-252 |
RB |
denotes |
previously |
T5755 |
253-262 |
VBN |
denotes |
described |
T5756 |
263-270 |
NNS |
denotes |
methods |
T5757 |
271-275 |
WDT |
denotes |
that |
T5758 |
276-281 |
VBP |
denotes |
yield |
T5759 |
282-292 |
JJ |
denotes |
cerebellar |
T5760 |
301-307 |
NN |
denotes |
neuron |
T5761 |
293-300 |
NN |
denotes |
granule |
T5762 |
308-316 |
VBN |
denotes |
enriched |
T5763 |
307-308 |
HYPH |
denotes |
- |
T5764 |
317-325 |
NNS |
denotes |
cultures |
T5765 |
326-327 |
-LRB- |
denotes |
[ |
T5766 |
327-329 |
CD |
denotes |
26 |
T5767 |
329-330 |
-RRB- |
denotes |
] |
T5768 |
330-331 |
. |
denotes |
. |
T5769 |
331-458 |
sentence |
denotes |
Postnatal day 4 (P4) cerebella were dissected in Hank's Balanced Salt Solution (HBSS, Sigma), supplemented with 35 mM glucose. |
T5770 |
332-341 |
JJ |
denotes |
Postnatal |
T5771 |
342-345 |
NN |
denotes |
day |
T5772 |
353-362 |
NNS |
denotes |
cerebella |
T5773 |
346-347 |
CD |
denotes |
4 |
T5774 |
348-349 |
-LRB- |
denotes |
( |
T5775 |
349-351 |
NN |
denotes |
P4 |
T5776 |
351-352 |
-RRB- |
denotes |
) |
T5777 |
368-377 |
VBN |
denotes |
dissected |
T5778 |
363-367 |
VBD |
denotes |
were |
T5779 |
378-380 |
IN |
denotes |
in |
T5780 |
381-385 |
NNP |
denotes |
Hank |
T5781 |
402-410 |
NNP |
denotes |
Solution |
T5782 |
385-387 |
POS |
denotes |
's |
T5783 |
388-396 |
NNP |
denotes |
Balanced |
T5784 |
397-401 |
NNP |
denotes |
Salt |
T5785 |
411-412 |
-LRB- |
denotes |
( |
T5786 |
412-416 |
NN |
denotes |
HBSS |
T5787 |
416-418 |
, |
denotes |
, |
T5788 |
418-423 |
NNP |
denotes |
Sigma |
T5789 |
423-424 |
-RRB- |
denotes |
) |
T5790 |
424-426 |
, |
denotes |
, |
T5791 |
426-438 |
VBN |
denotes |
supplemented |
T5792 |
439-443 |
IN |
denotes |
with |
T5793 |
444-446 |
CD |
denotes |
35 |
T5794 |
447-449 |
NN |
denotes |
mM |
T5795 |
450-457 |
NN |
denotes |
glucose |
T5796 |
457-458 |
. |
denotes |
. |
T5797 |
458-542 |
sentence |
denotes |
Tail biopsies were also collected for genomic DNA isolation and genotypic analysis. |
T5798 |
459-463 |
NN |
denotes |
Tail |
T5799 |
464-472 |
NNS |
denotes |
biopsies |
T5800 |
483-492 |
VBN |
denotes |
collected |
T5801 |
473-477 |
VBD |
denotes |
were |
T5802 |
478-482 |
RB |
denotes |
also |
T5803 |
493-496 |
IN |
denotes |
for |
T5804 |
497-504 |
JJ |
denotes |
genomic |
T5805 |
509-518 |
NN |
denotes |
isolation |
T5806 |
505-508 |
NN |
denotes |
DNA |
T5807 |
519-522 |
CC |
denotes |
and |
T5808 |
523-532 |
JJ |
denotes |
genotypic |
T5809 |
533-541 |
NN |
denotes |
analysis |
T5810 |
541-542 |
. |
denotes |
. |
T5811 |
542-736 |
sentence |
denotes |
Trypsin/EDTA (10 mg/ml, Sigma) and DNase I (100 μg/ml, Sigma), suspended in HBSS, helped dissociate cerebella for primary culture plating onto 0.01% poly-ornithine (Sigma) coated 100 mm dishes. |
T5812 |
543-550 |
NN |
denotes |
Trypsin |
T5813 |
551-555 |
NN |
denotes |
EDTA |
T5814 |
550-551 |
HYPH |
denotes |
/ |
T5815 |
625-631 |
VBD |
denotes |
helped |
T5816 |
556-557 |
-LRB- |
denotes |
( |
T5817 |
567-572 |
NNP |
denotes |
Sigma |
T5818 |
557-559 |
CD |
denotes |
10 |
T5819 |
560-562 |
NN |
denotes |
mg |
T5820 |
562-563 |
SYM |
denotes |
/ |
T5821 |
563-565 |
NN |
denotes |
ml |
T5822 |
565-567 |
, |
denotes |
, |
T5823 |
572-573 |
-RRB- |
denotes |
) |
T5824 |
574-577 |
CC |
denotes |
and |
T5825 |
578-583 |
NN |
denotes |
DNase |
T5826 |
584-585 |
CD |
denotes |
I |
T5827 |
586-587 |
-LRB- |
denotes |
( |
T5828 |
598-603 |
NNP |
denotes |
Sigma |
T5829 |
587-590 |
CD |
denotes |
100 |
T5830 |
591-593 |
NN |
denotes |
μg |
T5831 |
593-594 |
SYM |
denotes |
/ |
T5832 |
594-596 |
NN |
denotes |
ml |
T5833 |
596-598 |
, |
denotes |
, |
T5834 |
603-604 |
-RRB- |
denotes |
) |
T5835 |
604-606 |
, |
denotes |
, |
T5836 |
606-615 |
VBN |
denotes |
suspended |
T5837 |
616-618 |
IN |
denotes |
in |
T5838 |
619-623 |
NN |
denotes |
HBSS |
T5839 |
623-625 |
, |
denotes |
, |
T5840 |
632-642 |
VB |
denotes |
dissociate |
T5841 |
643-652 |
NNS |
denotes |
cerebella |
T5842 |
653-656 |
IN |
denotes |
for |
T5843 |
657-664 |
JJ |
denotes |
primary |
T5844 |
665-672 |
NN |
denotes |
culture |
T5845 |
673-680 |
NN |
denotes |
plating |
T5846 |
681-685 |
IN |
denotes |
onto |
T5847 |
686-690 |
CD |
denotes |
0.01 |
T5848 |
690-691 |
NN |
denotes |
% |
T5849 |
692-706 |
NN |
denotes |
poly-ornithine |
T5850 |
715-721 |
VBN |
denotes |
coated |
T5851 |
707-708 |
-LRB- |
denotes |
( |
T5852 |
708-713 |
NNP |
denotes |
Sigma |
T5853 |
713-714 |
-RRB- |
denotes |
) |
T5854 |
729-735 |
NNS |
denotes |
dishes |
T5855 |
722-725 |
CD |
denotes |
100 |
T5856 |
726-728 |
NN |
denotes |
mm |
T5857 |
735-736 |
. |
denotes |
. |
T5858 |
736-985 |
sentence |
denotes |
Primary cultures from individual cerebella were cultured overnight at 37°C, 5% CO2, in granule neuron growth media (Dulbecco's Modified Eagle Medium [DMEM, Gibco BRL #11995-065], 10% fetal bovine serum [Sigma #F-2442], supplemented with 24 mM KCl). |
T5859 |
737-744 |
JJ |
denotes |
Primary |
T5860 |
745-753 |
NNS |
denotes |
cultures |
T5861 |
785-793 |
VBN |
denotes |
cultured |
T5862 |
754-758 |
IN |
denotes |
from |
T5863 |
759-769 |
JJ |
denotes |
individual |
T5864 |
770-779 |
NNS |
denotes |
cerebella |
T5865 |
780-784 |
VBD |
denotes |
were |
T5866 |
794-803 |
RB |
denotes |
overnight |
T5867 |
804-806 |
IN |
denotes |
at |
T5868 |
807-809 |
CD |
denotes |
37 |
T5869 |
809-811 |
NN |
denotes |
°C |
T5870 |
811-813 |
, |
denotes |
, |
T5871 |
813-814 |
CD |
denotes |
5 |
T5872 |
814-815 |
NN |
denotes |
% |
T5873 |
816-819 |
NN |
denotes |
CO2 |
T5874 |
819-821 |
, |
denotes |
, |
T5875 |
821-823 |
IN |
denotes |
in |
T5876 |
824-831 |
NN |
denotes |
granule |
T5877 |
832-838 |
NN |
denotes |
neuron |
T5878 |
846-851 |
NNS |
denotes |
media |
T5879 |
839-845 |
NN |
denotes |
growth |
T5880 |
852-853 |
-LRB- |
denotes |
( |
T5881 |
853-861 |
NNP |
denotes |
Dulbecco |
T5882 |
879-885 |
NNP |
denotes |
Medium |
T5883 |
861-863 |
POS |
denotes |
's |
T5884 |
864-872 |
NNP |
denotes |
Modified |
T5885 |
873-878 |
NNP |
denotes |
Eagle |
T5886 |
886-887 |
-LRB- |
denotes |
[ |
T5887 |
887-891 |
NN |
denotes |
DMEM |
T5888 |
891-893 |
, |
denotes |
, |
T5889 |
893-898 |
NNP |
denotes |
Gibco |
T5890 |
903-904 |
NN |
denotes |
# |
T5891 |
899-902 |
NNP |
denotes |
BRL |
T5892 |
904-909 |
CD |
denotes |
11995 |
T5893 |
909-910 |
HYPH |
denotes |
- |
T5894 |
910-913 |
CD |
denotes |
065 |
T5895 |
913-914 |
-RRB- |
denotes |
] |
T5896 |
914-916 |
, |
denotes |
, |
T5897 |
916-918 |
CD |
denotes |
10 |
T5898 |
918-919 |
NN |
denotes |
% |
T5899 |
920-925 |
JJ |
denotes |
fetal |
T5900 |
933-938 |
NN |
denotes |
serum |
T5901 |
926-932 |
JJ |
denotes |
bovine |
T5902 |
939-940 |
-LRB- |
denotes |
[ |
T5903 |
940-945 |
NNP |
denotes |
Sigma |
T5904 |
947-948 |
NN |
denotes |
F |
T5905 |
946-947 |
NN |
denotes |
# |
T5906 |
948-949 |
HYPH |
denotes |
- |
T5907 |
949-953 |
CD |
denotes |
2442 |
T5908 |
953-954 |
-RRB- |
denotes |
] |
T5909 |
954-956 |
, |
denotes |
, |
T5910 |
956-968 |
VBN |
denotes |
supplemented |
T5911 |
969-973 |
IN |
denotes |
with |
T5912 |
974-976 |
CD |
denotes |
24 |
T5913 |
977-979 |
NN |
denotes |
mM |
T5914 |
980-983 |
NN |
denotes |
KCl |
T5915 |
983-984 |
-RRB- |
denotes |
) |
T5916 |
984-985 |
. |
denotes |
. |
T5917 |
985-1198 |
sentence |
denotes |
Infection was performed the following day with defective retrovirus transducing the temperature-sensitive tsA58/U19 large T antigen and a selection neomycin-resistance cassette [27], as previously described [28]. |
T5918 |
986-995 |
NN |
denotes |
Infection |
T5919 |
1000-1009 |
VBN |
denotes |
performed |
T5920 |
996-999 |
VBD |
denotes |
was |
T5921 |
1010-1013 |
DT |
denotes |
the |
T5922 |
1024-1027 |
NN |
denotes |
day |
T5923 |
1014-1023 |
JJ |
denotes |
following |
T5924 |
1028-1032 |
IN |
denotes |
with |
T5925 |
1033-1042 |
JJ |
denotes |
defective |
T5926 |
1043-1053 |
NN |
denotes |
retrovirus |
T5927 |
1054-1065 |
VBG |
denotes |
transducing |
T5928 |
1066-1069 |
DT |
denotes |
the |
T5929 |
1110-1117 |
NN |
denotes |
antigen |
T5930 |
1070-1081 |
NN |
denotes |
temperature |
T5931 |
1082-1091 |
JJ |
denotes |
sensitive |
T5932 |
1081-1082 |
HYPH |
denotes |
- |
T5933 |
1092-1097 |
NN |
denotes |
tsA58 |
T5934 |
1098-1101 |
NN |
denotes |
U19 |
T5935 |
1097-1098 |
HYPH |
denotes |
/ |
T5936 |
1102-1107 |
JJ |
denotes |
large |
T5937 |
1108-1109 |
NN |
denotes |
T |
T5938 |
1118-1121 |
CC |
denotes |
and |
T5939 |
1122-1123 |
DT |
denotes |
a |
T5940 |
1154-1162 |
NN |
denotes |
cassette |
T5941 |
1124-1133 |
NN |
denotes |
selection |
T5942 |
1134-1142 |
NN |
denotes |
neomycin |
T5943 |
1143-1153 |
NN |
denotes |
resistance |
T5944 |
1142-1143 |
HYPH |
denotes |
- |
T5945 |
1163-1164 |
-LRB- |
denotes |
[ |
T5946 |
1164-1166 |
CD |
denotes |
27 |
T5947 |
1166-1167 |
-RRB- |
denotes |
] |
T5948 |
1167-1169 |
, |
denotes |
, |
T5949 |
1169-1171 |
IN |
denotes |
as |
T5950 |
1183-1192 |
VBN |
denotes |
described |
T5951 |
1172-1182 |
RB |
denotes |
previously |
T5952 |
1193-1194 |
-LRB- |
denotes |
[ |
T5953 |
1194-1196 |
CD |
denotes |
28 |
T5954 |
1196-1197 |
-RRB- |
denotes |
] |
T5955 |
1197-1198 |
. |
denotes |
. |
T5956 |
1198-1370 |
sentence |
denotes |
Following infection, cultures were shifted to the tsA58/U19 permissive growth temperature of 33°C and selection was in the same growth media as above, with 200 μg/ml G418. |
T5957 |
1199-1208 |
VBG |
denotes |
Following |
T5958 |
1234-1241 |
VBN |
denotes |
shifted |
T5959 |
1209-1218 |
NN |
denotes |
infection |
T5960 |
1218-1220 |
, |
denotes |
, |
T5961 |
1220-1228 |
NNS |
denotes |
cultures |
T5962 |
1229-1233 |
VBD |
denotes |
were |
T5963 |
1242-1244 |
IN |
denotes |
to |
T5964 |
1245-1248 |
DT |
denotes |
the |
T5965 |
1277-1288 |
NN |
denotes |
temperature |
T5966 |
1249-1254 |
NN |
denotes |
tsA58 |
T5967 |
1255-1258 |
NN |
denotes |
U19 |
T5968 |
1254-1255 |
HYPH |
denotes |
/ |
T5969 |
1259-1269 |
JJ |
denotes |
permissive |
T5970 |
1270-1276 |
NN |
denotes |
growth |
T5971 |
1289-1291 |
IN |
denotes |
of |
T5972 |
1292-1294 |
CD |
denotes |
33 |
T5973 |
1294-1296 |
NN |
denotes |
°C |
T5974 |
1297-1300 |
CC |
denotes |
and |
T5975 |
1301-1310 |
NN |
denotes |
selection |
T5976 |
1311-1314 |
VBD |
denotes |
was |
T5977 |
1315-1317 |
IN |
denotes |
in |
T5978 |
1318-1321 |
DT |
denotes |
the |
T5979 |
1334-1339 |
NNS |
denotes |
media |
T5980 |
1322-1326 |
JJ |
denotes |
same |
T5981 |
1327-1333 |
NN |
denotes |
growth |
T5982 |
1340-1342 |
IN |
denotes |
as |
T5983 |
1343-1348 |
RB |
denotes |
above |
T5984 |
1348-1350 |
, |
denotes |
, |
T5985 |
1350-1354 |
IN |
denotes |
with |
T5986 |
1355-1358 |
CD |
denotes |
200 |
T5987 |
1359-1361 |
NN |
denotes |
μg |
T5988 |
1365-1369 |
NN |
denotes |
G418 |
T5989 |
1361-1362 |
SYM |
denotes |
/ |
T5990 |
1362-1364 |
NN |
denotes |
ml |
T5991 |
1369-1370 |
. |
denotes |
. |
T5992 |
1370-1509 |
sentence |
denotes |
Conditionally immortalized colonies were isolated 3–9 weeks post-infection and expanded for frozen stocks and further sub-clone isolation. |
T5993 |
1371-1384 |
RB |
denotes |
Conditionally |
T5994 |
1385-1397 |
VBN |
denotes |
immortalized |
T5995 |
1398-1406 |
NNS |
denotes |
colonies |
T5996 |
1412-1420 |
VBN |
denotes |
isolated |
T5997 |
1407-1411 |
VBD |
denotes |
were |
T5998 |
1421-1422 |
CD |
denotes |
3 |
T5999 |
1423-1424 |
CD |
denotes |
9 |
T6000 |
1422-1423 |
SYM |
denotes |
– |
T6001 |
1425-1430 |
NNS |
denotes |
weeks |
T6002 |
1431-1445 |
RB |
denotes |
post-infection |
T6003 |
1446-1449 |
CC |
denotes |
and |
T6004 |
1450-1458 |
VBN |
denotes |
expanded |
T6005 |
1459-1462 |
IN |
denotes |
for |
T6006 |
1463-1469 |
JJ |
denotes |
frozen |
T6007 |
1470-1476 |
NNS |
denotes |
stocks |
T6008 |
1477-1480 |
CC |
denotes |
and |
T6009 |
1481-1488 |
JJ |
denotes |
further |
T6010 |
1499-1508 |
NN |
denotes |
isolation |
T6011 |
1489-1498 |
NN |
denotes |
sub-clone |
T6012 |
1508-1509 |
. |
denotes |
. |
T6013 |
1509-1642 |
sentence |
denotes |
Multiple clonal lines were obtained for each genotype and all phenotypes were confirmed in at least 2 independent CbCln3 cell lines. |
T6014 |
1510-1518 |
JJ |
denotes |
Multiple |
T6015 |
1526-1531 |
NNS |
denotes |
lines |
T6016 |
1519-1525 |
JJ |
denotes |
clonal |
T6017 |
1537-1545 |
VBN |
denotes |
obtained |
T6018 |
1532-1536 |
VBD |
denotes |
were |
T6019 |
1546-1549 |
IN |
denotes |
for |
T6020 |
1550-1554 |
DT |
denotes |
each |
T6021 |
1555-1563 |
NN |
denotes |
genotype |
T6022 |
1564-1567 |
CC |
denotes |
and |
T6023 |
1568-1571 |
DT |
denotes |
all |
T6024 |
1572-1582 |
NNS |
denotes |
phenotypes |
T6025 |
1583-1587 |
VBD |
denotes |
were |
T6026 |
1588-1597 |
VBN |
denotes |
confirmed |
T6027 |
1598-1600 |
IN |
denotes |
in |
T6028 |
1601-1603 |
RB |
denotes |
at |
T6029 |
1610-1611 |
CD |
denotes |
2 |
T6030 |
1604-1609 |
RBS |
denotes |
least |
T6031 |
1636-1641 |
NNS |
denotes |
lines |
T6032 |
1612-1623 |
JJ |
denotes |
independent |
T6033 |
1624-1630 |
NN |
denotes |
CbCln3 |
T6034 |
1631-1635 |
NN |
denotes |
cell |
T6035 |
1641-1642 |
. |
denotes |
. |
T6036 |
1642-1767 |
sentence |
denotes |
CbCln3 cell lines were maintained on 0.01% poly-ornithine coated dishes at 30–90% confluency, in 33°C and 5% CO2 atmosphere. |
T6037 |
1643-1649 |
NN |
denotes |
CbCln3 |
T6038 |
1655-1660 |
NNS |
denotes |
lines |
T6039 |
1650-1654 |
NN |
denotes |
cell |
T6040 |
1666-1676 |
VBN |
denotes |
maintained |
T6041 |
1661-1665 |
VBD |
denotes |
were |
T6042 |
1677-1679 |
IN |
denotes |
on |
T6043 |
1680-1684 |
CD |
denotes |
0.01 |
T6044 |
1684-1685 |
NN |
denotes |
% |
T6045 |
1708-1714 |
NNS |
denotes |
dishes |
T6046 |
1686-1690 |
NN |
denotes |
poly |
T6047 |
1691-1700 |
NN |
denotes |
ornithine |
T6048 |
1690-1691 |
HYPH |
denotes |
- |
T6049 |
1701-1707 |
VBN |
denotes |
coated |
T6050 |
1715-1717 |
IN |
denotes |
at |
T6051 |
1718-1720 |
CD |
denotes |
30 |
T6052 |
1721-1723 |
CD |
denotes |
90 |
T6053 |
1720-1721 |
SYM |
denotes |
– |
T6054 |
1723-1724 |
NN |
denotes |
% |
T6055 |
1725-1735 |
NN |
denotes |
confluency |
T6056 |
1735-1737 |
, |
denotes |
, |
T6057 |
1737-1739 |
IN |
denotes |
in |
T6058 |
1740-1742 |
CD |
denotes |
33 |
T6059 |
1742-1744 |
NN |
denotes |
°C |
T6060 |
1745-1748 |
CC |
denotes |
and |
T6061 |
1749-1750 |
CD |
denotes |
5 |
T6062 |
1756-1766 |
NN |
denotes |
atmosphere |
T6063 |
1750-1751 |
SYM |
denotes |
% |
T6064 |
1752-1755 |
NN |
denotes |
CO2 |
T6065 |
1766-1767 |
. |
denotes |
. |
T6066 |
1767-1858 |
sentence |
denotes |
Passage number was recorded (up to ~20 passages), but had no apparent impact on phenotype. |
T6067 |
1768-1775 |
NN |
denotes |
Passage |
T6068 |
1776-1782 |
NN |
denotes |
number |
T6069 |
1787-1795 |
VBN |
denotes |
recorded |
T6070 |
1783-1786 |
VBD |
denotes |
was |
T6071 |
1796-1797 |
-LRB- |
denotes |
( |
T6072 |
1807-1815 |
NNS |
denotes |
passages |
T6073 |
1797-1799 |
IN |
denotes |
up |
T6074 |
1804-1806 |
CD |
denotes |
20 |
T6075 |
1800-1802 |
IN |
denotes |
to |
T6076 |
1803-1804 |
SYM |
denotes |
~ |
T6077 |
1815-1816 |
-RRB- |
denotes |
) |
T6078 |
1816-1818 |
, |
denotes |
, |
T6079 |
1818-1821 |
CC |
denotes |
but |
T6080 |
1822-1825 |
VBD |
denotes |
had |
T6081 |
1826-1828 |
DT |
denotes |
no |
T6082 |
1838-1844 |
NN |
denotes |
impact |
T6083 |
1829-1837 |
JJ |
denotes |
apparent |
T6084 |
1845-1847 |
IN |
denotes |
on |
T6085 |
1848-1857 |
NN |
denotes |
phenotype |
T6086 |
1857-1858 |
. |
denotes |
. |
T6087 |
1858-2026 |
sentence |
denotes |
Neuronal differentiation was as previously described [29] with the following cocktail: 10 ng/ml α-FGF, 250 μM IBMX, 200 nM TPA, 50 μM forskolin, 5 μM dopamine (Sigma). |
T6088 |
1859-1867 |
JJ |
denotes |
Neuronal |
T6089 |
1868-1883 |
NN |
denotes |
differentiation |
T6090 |
1884-1887 |
VBD |
denotes |
was |
T6091 |
1888-1890 |
IN |
denotes |
as |
T6092 |
1902-1911 |
VBN |
denotes |
described |
T6093 |
1891-1901 |
RB |
denotes |
previously |
T6094 |
1912-1913 |
-LRB- |
denotes |
[ |
T6095 |
1913-1915 |
CD |
denotes |
29 |
T6096 |
1915-1916 |
-RRB- |
denotes |
] |
T6097 |
1917-1921 |
IN |
denotes |
with |
T6098 |
1922-1925 |
DT |
denotes |
the |
T6099 |
1936-1944 |
NN |
denotes |
cocktail |
T6100 |
1926-1935 |
JJ |
denotes |
following |
T6101 |
1944-1946 |
: |
denotes |
: |
T6102 |
1946-1948 |
CD |
denotes |
10 |
T6103 |
1949-1951 |
NN |
denotes |
ng |
T6104 |
1957-1960 |
NN |
denotes |
FGF |
T6105 |
1951-1952 |
SYM |
denotes |
/ |
T6106 |
1952-1954 |
NN |
denotes |
ml |
T6107 |
1955-1956 |
NN |
denotes |
α |
T6108 |
1956-1957 |
HYPH |
denotes |
- |
T6109 |
1960-1962 |
, |
denotes |
, |
T6110 |
1962-1965 |
CD |
denotes |
250 |
T6111 |
1966-1968 |
NN |
denotes |
μM |
T6112 |
1969-1973 |
NN |
denotes |
IBMX |
T6113 |
1973-1975 |
, |
denotes |
, |
T6114 |
1975-1978 |
CD |
denotes |
200 |
T6115 |
1979-1981 |
NN |
denotes |
nM |
T6116 |
1982-1985 |
NN |
denotes |
TPA |
T6117 |
1985-1987 |
, |
denotes |
, |
T6118 |
1987-1989 |
CD |
denotes |
50 |
T6119 |
1990-1992 |
NN |
denotes |
μM |
T6120 |
1993-2002 |
NN |
denotes |
forskolin |
T6121 |
2002-2004 |
, |
denotes |
, |
T6122 |
2004-2005 |
CD |
denotes |
5 |
T6123 |
2006-2008 |
NN |
denotes |
μM |
T6124 |
2009-2017 |
NN |
denotes |
dopamine |
T6125 |
2018-2019 |
-LRB- |
denotes |
( |
T6126 |
2019-2024 |
NNP |
denotes |
Sigma |
T6127 |
2024-2025 |
-RRB- |
denotes |
) |
T6128 |
2025-2026 |
. |
denotes |
. |
R3332 |
T5714 |
T5713 |
punct |
", ",Generation |
R3333 |
T5715 |
T5713 |
conj |
maintenance,Generation |
R3334 |
T5716 |
T5715 |
cc |
and,maintenance |
R3335 |
T5717 |
T5715 |
conj |
differentiation,maintenance |
R3336 |
T5718 |
T5713 |
prep |
of,Generation |
R3337 |
T5719 |
T5720 |
nmod |
CbCln3,lines |
R3338 |
T5720 |
T5718 |
pobj |
lines,of |
R3339 |
T5721 |
T5720 |
amod |
cerebellar,lines |
R3340 |
T5722 |
T5723 |
amod |
neuronal,precursor |
R3341 |
T5723 |
T5720 |
compound |
precursor,lines |
R3342 |
T5725 |
T5726 |
nmod |
Cln3Δex7,mice |
R3343 |
T5726 |
T5732 |
nsubjpass |
mice,described |
R3344 |
T5727 |
T5725 |
punct |
/,Cln3Δex7 |
R3345 |
T5728 |
T5725 |
nummod |
8,Cln3Δex7 |
R3346 |
T5729 |
T5726 |
amod |
knock,mice |
R3347 |
T5730 |
T5729 |
punct |
-,knock |
R3348 |
T5731 |
T5729 |
prt |
in,knock |
R3349 |
T5733 |
T5732 |
aux |
have,described |
R3350 |
T5734 |
T5732 |
auxpass |
been,described |
R3351 |
T5735 |
T5732 |
advmod |
previously,described |
R3352 |
T5736 |
T5737 |
punct |
[,12 |
R3353 |
T5737 |
T5732 |
parataxis |
12,described |
R3354 |
T5738 |
T5737 |
punct |
],12 |
R3355 |
T5739 |
T5732 |
punct |
.,described |
R3356 |
T5741 |
T5742 |
compound |
Littermate,pups |
R3357 |
T5742 |
T5743 |
nsubjpass |
pups,used |
R3358 |
T5744 |
T5742 |
prep |
from,pups |
R3359 |
T5745 |
T5746 |
compound |
heterozygote,crosses |
R3360 |
T5746 |
T5744 |
pobj |
crosses,from |
R3361 |
T5747 |
T5743 |
auxpass |
were,used |
R3362 |
T5748 |
T5743 |
prep |
for,used |
R3363 |
T5749 |
T5750 |
amod |
primary,establishment |
R3364 |
T5750 |
T5748 |
pobj |
establishment,for |
R3365 |
T5751 |
T5750 |
compound |
culture,establishment |
R3366 |
T5752 |
T5743 |
punct |
", ",used |
R3367 |
T5753 |
T5743 |
prep |
by,used |
R3368 |
T5754 |
T5755 |
advmod |
previously,described |
R3369 |
T5755 |
T5756 |
amod |
described,methods |
R3370 |
T5756 |
T5753 |
pobj |
methods,by |
R3371 |
T5757 |
T5758 |
dep |
that,yield |
R3372 |
T5758 |
T5756 |
relcl |
yield,methods |
R3373 |
T5759 |
T5760 |
amod |
cerebellar,neuron |
R3374 |
T5760 |
T5762 |
npadvmod |
neuron,enriched |
R3375 |
T5761 |
T5760 |
compound |
granule,neuron |
R3376 |
T5762 |
T5764 |
amod |
enriched,cultures |
R3377 |
T5763 |
T5762 |
punct |
-,enriched |
R3378 |
T5764 |
T5758 |
dobj |
cultures,yield |
R3379 |
T5765 |
T5766 |
punct |
[,26 |
R3380 |
T5766 |
T5758 |
parataxis |
26,yield |
R3381 |
T5767 |
T5766 |
punct |
],26 |
R3382 |
T5768 |
T5743 |
punct |
.,used |
R3383 |
T5770 |
T5771 |
amod |
Postnatal,day |
R3384 |
T5771 |
T5772 |
nmod |
day,cerebella |
R3385 |
T5772 |
T5777 |
nsubjpass |
cerebella,dissected |
R3386 |
T5773 |
T5771 |
nummod |
4,day |
R3387 |
T5774 |
T5771 |
punct |
(,day |
R3388 |
T5775 |
T5771 |
appos |
P4,day |
R3389 |
T5776 |
T5772 |
punct |
),cerebella |
R3390 |
T5778 |
T5777 |
auxpass |
were,dissected |
R3391 |
T5779 |
T5777 |
prep |
in,dissected |
R3392 |
T5780 |
T5781 |
poss |
Hank,Solution |
R3393 |
T5781 |
T5779 |
pobj |
Solution,in |
R3394 |
T5782 |
T5780 |
case |
's,Hank |
R3395 |
T5783 |
T5781 |
compound |
Balanced,Solution |
R3396 |
T5784 |
T5781 |
compound |
Salt,Solution |
R3397 |
T5785 |
T5781 |
punct |
(,Solution |
R3398 |
T5786 |
T5781 |
appos |
HBSS,Solution |
R3399 |
T5787 |
T5788 |
punct |
", ",Sigma |
R3400 |
T5788 |
T5781 |
parataxis |
Sigma,Solution |
R3401 |
T5789 |
T5788 |
punct |
),Sigma |
R3402 |
T5790 |
T5781 |
punct |
", ",Solution |
R3403 |
T5791 |
T5781 |
acl |
supplemented,Solution |
R3404 |
T5792 |
T5791 |
prep |
with,supplemented |
R3405 |
T5793 |
T5794 |
nummod |
35,mM |
R3406 |
T5794 |
T5795 |
compound |
mM,glucose |
R3407 |
T5795 |
T5792 |
pobj |
glucose,with |
R3408 |
T5796 |
T5777 |
punct |
.,dissected |
R3409 |
T5798 |
T5799 |
compound |
Tail,biopsies |
R3410 |
T5799 |
T5800 |
nsubjpass |
biopsies,collected |
R3411 |
T5801 |
T5800 |
auxpass |
were,collected |
R3412 |
T5802 |
T5800 |
advmod |
also,collected |
R3413 |
T5803 |
T5800 |
prep |
for,collected |
R3414 |
T5804 |
T5805 |
amod |
genomic,isolation |
R3415 |
T5805 |
T5803 |
pobj |
isolation,for |
R3416 |
T5806 |
T5805 |
compound |
DNA,isolation |
R3417 |
T5807 |
T5805 |
cc |
and,isolation |
R3418 |
T5808 |
T5809 |
amod |
genotypic,analysis |
R3419 |
T5809 |
T5805 |
conj |
analysis,isolation |
R3420 |
T5810 |
T5800 |
punct |
.,collected |
R3421 |
T5812 |
T5813 |
compound |
Trypsin,EDTA |
R3422 |
T5813 |
T5815 |
nsubj |
EDTA,helped |
R3423 |
T5814 |
T5813 |
punct |
/,EDTA |
R3424 |
T5816 |
T5817 |
punct |
(,Sigma |
R3425 |
T5817 |
T5813 |
parataxis |
Sigma,EDTA |
R3426 |
T5818 |
T5819 |
nummod |
10,mg |
R3427 |
T5819 |
T5817 |
dep |
mg,Sigma |
R3428 |
T5820 |
T5821 |
punct |
/,ml |
R3429 |
T5821 |
T5819 |
prep |
ml,mg |
R3430 |
T5822 |
T5817 |
punct |
", ",Sigma |
R3431 |
T5823 |
T5817 |
punct |
),Sigma |
R3432 |
T5824 |
T5813 |
cc |
and,EDTA |
R3433 |
T5825 |
T5813 |
conj |
DNase,EDTA |
R3434 |
T5826 |
T5825 |
nummod |
I,DNase |
R3435 |
T5827 |
T5828 |
punct |
(,Sigma |
R3436 |
T5828 |
T5825 |
parataxis |
Sigma,DNase |
R3437 |
T5829 |
T5830 |
nummod |
100,μg |
R3438 |
T5830 |
T5828 |
dep |
μg,Sigma |
R3439 |
T5831 |
T5832 |
punct |
/,ml |
R3440 |
T5832 |
T5830 |
prep |
ml,μg |
R3441 |
T5833 |
T5828 |
punct |
", ",Sigma |
R3442 |
T5834 |
T5828 |
punct |
),Sigma |
R3443 |
T5835 |
T5813 |
punct |
", ",EDTA |
R3444 |
T5836 |
T5813 |
acl |
suspended,EDTA |
R3445 |
T5837 |
T5836 |
prep |
in,suspended |
R3446 |
T5838 |
T5837 |
pobj |
HBSS,in |
R3447 |
T5839 |
T5815 |
punct |
", ",helped |
R3448 |
T5840 |
T5815 |
xcomp |
dissociate,helped |
R3449 |
T5841 |
T5840 |
dobj |
cerebella,dissociate |
R3450 |
T5842 |
T5840 |
prep |
for,dissociate |
R3451 |
T5843 |
T5844 |
amod |
primary,culture |
R3452 |
T5844 |
T5845 |
compound |
culture,plating |
R3453 |
T5845 |
T5842 |
pobj |
plating,for |
R3454 |
T5846 |
T5845 |
prep |
onto,plating |
R3455 |
T5847 |
T5848 |
nummod |
0.01,% |
R3456 |
T5848 |
T5849 |
compound |
%,poly-ornithine |
R3457 |
T5849 |
T5850 |
npadvmod |
poly-ornithine,coated |
R3458 |
T5850 |
T5854 |
amod |
coated,dishes |
R3459 |
T5851 |
T5852 |
punct |
(,Sigma |
R3460 |
T5852 |
T5849 |
parataxis |
Sigma,poly-ornithine |
R3461 |
T5853 |
T5852 |
punct |
),Sigma |
R3462 |
T5854 |
T5846 |
pobj |
dishes,onto |
R3463 |
T5855 |
T5856 |
nummod |
100,mm |
R3464 |
T5856 |
T5854 |
compound |
mm,dishes |
R3465 |
T5857 |
T5815 |
punct |
.,helped |
R3466 |
T5859 |
T5860 |
amod |
Primary,cultures |
R3467 |
T5860 |
T5861 |
nsubjpass |
cultures,cultured |
R3468 |
T5862 |
T5860 |
prep |
from,cultures |
R3469 |
T5863 |
T5864 |
amod |
individual,cerebella |
R3470 |
T5864 |
T5862 |
pobj |
cerebella,from |
R3471 |
T5865 |
T5861 |
auxpass |
were,cultured |
R3472 |
T5866 |
T5861 |
advmod |
overnight,cultured |
R3473 |
T5867 |
T5861 |
prep |
at,cultured |
R3474 |
T5868 |
T5869 |
nummod |
37,°C |
R3475 |
T5869 |
T5867 |
pobj |
°C,at |
R3476 |
T5870 |
T5869 |
punct |
", ",°C |
R3477 |
T5871 |
T5872 |
nummod |
5,% |
R3478 |
T5872 |
T5873 |
compound |
%,CO2 |
R3479 |
T5873 |
T5869 |
appos |
CO2,°C |
R3480 |
T5874 |
T5861 |
punct |
", ",cultured |
R3481 |
T5875 |
T5861 |
prep |
in,cultured |
R3482 |
T5876 |
T5877 |
compound |
granule,neuron |
R3483 |
T5877 |
T5878 |
compound |
neuron,media |
R3484 |
T5878 |
T5875 |
pobj |
media,in |
R3485 |
T5879 |
T5878 |
compound |
growth,media |
R3486 |
T5880 |
T5878 |
punct |
(,media |
R3487 |
T5881 |
T5882 |
poss |
Dulbecco,Medium |
R3488 |
T5882 |
T5878 |
appos |
Medium,media |
R3489 |
T5883 |
T5881 |
case |
's,Dulbecco |
R3490 |
T5884 |
T5882 |
compound |
Modified,Medium |
R3491 |
T5885 |
T5882 |
compound |
Eagle,Medium |
R3492 |
T5886 |
T5882 |
punct |
[,Medium |
R3493 |
T5887 |
T5882 |
appos |
DMEM,Medium |
R3494 |
T5888 |
T5882 |
punct |
", ",Medium |
R3495 |
T5889 |
T5890 |
compound |
Gibco,# |
R3496 |
T5890 |
T5882 |
appos |
#,Medium |
R3497 |
T5891 |
T5890 |
compound |
BRL,# |
R3498 |
T5892 |
T5890 |
nummod |
11995,# |
R3499 |
T5893 |
T5890 |
punct |
-,# |
R3500 |
T5894 |
T5890 |
nummod |
065,# |
R3501 |
T5895 |
T5882 |
punct |
],Medium |
R3502 |
T5896 |
T5882 |
punct |
", ",Medium |
R3503 |
T5897 |
T5898 |
nummod |
10,% |
R3504 |
T5898 |
T5882 |
appos |
%,Medium |
R3505 |
T5899 |
T5900 |
amod |
fetal,serum |
R3506 |
T5900 |
T5898 |
appos |
serum,% |
R3507 |
T5901 |
T5900 |
amod |
bovine,serum |
R3508 |
T5902 |
T5900 |
punct |
[,serum |
R3509 |
T5903 |
T5904 |
compound |
Sigma,F |
R3510 |
T5904 |
T5900 |
appos |
F,serum |
R3511 |
T5905 |
T5904 |
compound |
#,F |
R3512 |
T5906 |
T5904 |
punct |
-,F |
R3513 |
T5907 |
T5904 |
nummod |
2442,F |
R3514 |
T5908 |
T5882 |
punct |
],Medium |
R3515 |
T5909 |
T5882 |
punct |
", ",Medium |
R3516 |
T5910 |
T5882 |
acl |
supplemented,Medium |
R3517 |
T5911 |
T5910 |
prep |
with,supplemented |
R3518 |
T5912 |
T5913 |
nummod |
24,mM |
R3519 |
T5913 |
T5914 |
compound |
mM,KCl |
R3520 |
T5914 |
T5911 |
pobj |
KCl,with |
R3521 |
T5915 |
T5861 |
punct |
),cultured |
R3522 |
T5916 |
T5861 |
punct |
.,cultured |
R3523 |
T5918 |
T5919 |
nsubjpass |
Infection,performed |
R3524 |
T5920 |
T5919 |
auxpass |
was,performed |
R3525 |
T5921 |
T5922 |
det |
the,day |
R3526 |
T5922 |
T5919 |
npadvmod |
day,performed |
R3527 |
T5923 |
T5922 |
amod |
following,day |
R3528 |
T5924 |
T5919 |
prep |
with,performed |
R3529 |
T5925 |
T5926 |
amod |
defective,retrovirus |
R3530 |
T5926 |
T5927 |
nsubj |
retrovirus,transducing |
R3531 |
T5927 |
T5924 |
pcomp |
transducing,with |
R3532 |
T5928 |
T5929 |
det |
the,antigen |
R3533 |
T5929 |
T5927 |
dobj |
antigen,transducing |
R3534 |
T5930 |
T5931 |
npadvmod |
temperature,sensitive |
R3535 |
T5931 |
T5929 |
amod |
sensitive,antigen |
R3536 |
T5932 |
T5931 |
punct |
-,sensitive |
R3537 |
T5933 |
T5934 |
nmod |
tsA58,U19 |
R3538 |
T5934 |
T5929 |
nmod |
U19,antigen |
R3539 |
T5935 |
T5934 |
punct |
/,U19 |
R3540 |
T5936 |
T5929 |
amod |
large,antigen |
R3541 |
T5937 |
T5929 |
compound |
T,antigen |
R3542 |
T5938 |
T5929 |
cc |
and,antigen |
R3543 |
T5939 |
T5940 |
det |
a,cassette |
R3544 |
T5940 |
T5929 |
conj |
cassette,antigen |
R3545 |
T5941 |
T5940 |
compound |
selection,cassette |
R3546 |
T5942 |
T5943 |
compound |
neomycin,resistance |
R3547 |
T5943 |
T5940 |
compound |
resistance,cassette |
R3548 |
T5944 |
T5943 |
punct |
-,resistance |
R3549 |
T5945 |
T5946 |
punct |
[,27 |
R3550 |
T5946 |
T5940 |
parataxis |
27,cassette |
R3551 |
T5947 |
T5946 |
punct |
],27 |
R3552 |
T5948 |
T5927 |
punct |
", ",transducing |
R3553 |
T5949 |
T5950 |
mark |
as,described |
R3554 |
T5950 |
T5927 |
advcl |
described,transducing |
R3555 |
T5951 |
T5950 |
advmod |
previously,described |
R3556 |
T5952 |
T5953 |
punct |
[,28 |
R3557 |
T5953 |
T5950 |
parataxis |
28,described |
R3558 |
T5954 |
T5953 |
punct |
],28 |
R3559 |
T5955 |
T5919 |
punct |
.,performed |
R3560 |
T5957 |
T5958 |
prep |
Following,shifted |
R3561 |
T5959 |
T5957 |
pobj |
infection,Following |
R3562 |
T5960 |
T5958 |
punct |
", ",shifted |
R3563 |
T5961 |
T5958 |
nsubjpass |
cultures,shifted |
R3564 |
T5962 |
T5958 |
auxpass |
were,shifted |
R3565 |
T5963 |
T5958 |
prep |
to,shifted |
R3566 |
T5964 |
T5965 |
det |
the,temperature |
R3567 |
T5965 |
T5963 |
pobj |
temperature,to |
R3568 |
T5966 |
T5967 |
compound |
tsA58,U19 |
R3569 |
T5967 |
T5969 |
npadvmod |
U19,permissive |
R3570 |
T5968 |
T5967 |
punct |
/,U19 |
R3571 |
T5969 |
T5965 |
amod |
permissive,temperature |
R3572 |
T5970 |
T5965 |
compound |
growth,temperature |
R3573 |
T5971 |
T5965 |
prep |
of,temperature |
R3574 |
T5972 |
T5973 |
nummod |
33,°C |
R3575 |
T5973 |
T5971 |
pobj |
°C,of |
R3576 |
T5974 |
T5958 |
cc |
and,shifted |
R3577 |
T5975 |
T5976 |
nsubj |
selection,was |
R3578 |
T5976 |
T5958 |
conj |
was,shifted |
R3579 |
T5977 |
T5976 |
prep |
in,was |
R3580 |
T5978 |
T5979 |
det |
the,media |
R3581 |
T5979 |
T5977 |
pobj |
media,in |
R3582 |
T5980 |
T5979 |
amod |
same,media |
R3583 |
T5981 |
T5979 |
compound |
growth,media |
R3584 |
T5982 |
T5979 |
prep |
as,media |
R3585 |
T5983 |
T5982 |
pcomp |
above,as |
R3586 |
T5984 |
T5976 |
punct |
", ",was |
R3587 |
T5985 |
T5976 |
prep |
with,was |
R3588 |
T5986 |
T5987 |
nummod |
200,μg |
R3589 |
T5987 |
T5988 |
nmod |
μg,G418 |
R3590 |
T5988 |
T5985 |
pobj |
G418,with |
R3591 |
T5989 |
T5990 |
punct |
/,ml |
R3592 |
T5990 |
T5987 |
prep |
ml,μg |
R3593 |
T5991 |
T5976 |
punct |
.,was |
R3594 |
T5993 |
T5994 |
advmod |
Conditionally,immortalized |
R3595 |
T5994 |
T5995 |
amod |
immortalized,colonies |
R3596 |
T5995 |
T5996 |
nsubjpass |
colonies,isolated |
R3597 |
T5997 |
T5996 |
auxpass |
were,isolated |
R3598 |
T5998 |
T5999 |
quantmod |
3,9 |
R3599 |
T5999 |
T6001 |
nummod |
9,weeks |
R3600 |
T6000 |
T5999 |
punct |
–,9 |
R3601 |
T6001 |
T6002 |
npadvmod |
weeks,post-infection |
R3602 |
T6002 |
T5996 |
advmod |
post-infection,isolated |
R3603 |
T6003 |
T6004 |
cc |
and,expanded |
R3604 |
T6004 |
T5996 |
dep |
expanded,isolated |
R3605 |
T6005 |
T6004 |
prep |
for,expanded |
R3606 |
T6006 |
T6007 |
amod |
frozen,stocks |
R3607 |
T6007 |
T6005 |
pobj |
stocks,for |
R3608 |
T6008 |
T6007 |
cc |
and,stocks |
R3609 |
T6009 |
T6010 |
amod |
further,isolation |
R3610 |
T6010 |
T6007 |
conj |
isolation,stocks |
R3611 |
T6011 |
T6010 |
compound |
sub-clone,isolation |
R3612 |
T6012 |
T5996 |
punct |
.,isolated |
R3613 |
T6014 |
T6015 |
amod |
Multiple,lines |
R3614 |
T6015 |
T6017 |
nsubjpass |
lines,obtained |
R3615 |
T6016 |
T6015 |
amod |
clonal,lines |
R3616 |
T6018 |
T6017 |
auxpass |
were,obtained |
R3617 |
T6019 |
T6017 |
prep |
for,obtained |
R3618 |
T6020 |
T6021 |
det |
each,genotype |
R3619 |
T6021 |
T6019 |
pobj |
genotype,for |
R3620 |
T6022 |
T6021 |
cc |
and,genotype |
R3621 |
T6023 |
T6024 |
det |
all,phenotypes |
R3622 |
T6024 |
T6021 |
conj |
phenotypes,genotype |
R3623 |
T6025 |
T6026 |
auxpass |
were,confirmed |
R3624 |
T6026 |
T6017 |
dep |
confirmed,obtained |
R3625 |
T6027 |
T6026 |
prep |
in,confirmed |
R3626 |
T6028 |
T6029 |
advmod |
at,2 |
R3627 |
T6029 |
T6031 |
nummod |
2,lines |
R3628 |
T6030 |
T6029 |
advmod |
least,2 |
R3629 |
T6031 |
T6027 |
pobj |
lines,in |
R3630 |
T6032 |
T6031 |
amod |
independent,lines |
R3631 |
T6033 |
T6031 |
compound |
CbCln3,lines |
R3632 |
T6034 |
T6031 |
compound |
cell,lines |
R3633 |
T6035 |
T6017 |
punct |
.,obtained |
R3634 |
T6037 |
T6038 |
compound |
CbCln3,lines |
R3635 |
T6038 |
T6040 |
nsubjpass |
lines,maintained |
R3636 |
T6039 |
T6038 |
compound |
cell,lines |
R3637 |
T6041 |
T6040 |
auxpass |
were,maintained |
R3638 |
T6042 |
T6040 |
prep |
on,maintained |
R3639 |
T6043 |
T6044 |
nummod |
0.01,% |
R3640 |
T6044 |
T6045 |
nmod |
%,dishes |
R3641 |
T6045 |
T6042 |
pobj |
dishes,on |
R3642 |
T6046 |
T6047 |
compound |
poly,ornithine |
R3643 |
T6047 |
T6049 |
npadvmod |
ornithine,coated |
R3644 |
T6048 |
T6047 |
punct |
-,ornithine |
R3645 |
T6049 |
T6045 |
amod |
coated,dishes |
R3646 |
T6050 |
T6040 |
prep |
at,maintained |
R3647 |
T6051 |
T6052 |
quantmod |
30,90 |
R3648 |
T6052 |
T6054 |
nummod |
90,% |
R3649 |
T6053 |
T6052 |
punct |
–,90 |
R3650 |
T6054 |
T6055 |
compound |
%,confluency |
R3651 |
T6055 |
T6050 |
pobj |
confluency,at |
R3652 |
T6056 |
T6040 |
punct |
", ",maintained |
R3653 |
T6057 |
T6040 |
prep |
in,maintained |
R3654 |
T6058 |
T6059 |
nummod |
33,°C |
R3655 |
T6059 |
T6057 |
pobj |
°C,in |
R3656 |
T6060 |
T6059 |
cc |
and,°C |
R3657 |
T6061 |
T6062 |
nummod |
5,atmosphere |
R3658 |
T6062 |
T6059 |
conj |
atmosphere,°C |
R3659 |
T6063 |
T6061 |
punct |
%,5 |
R3660 |
T6064 |
T6062 |
compound |
CO2,atmosphere |
R3661 |
T6065 |
T6040 |
punct |
.,maintained |
R3662 |
T6067 |
T6068 |
compound |
Passage,number |
R3663 |
T6068 |
T6069 |
nsubjpass |
number,recorded |
R3664 |
T6070 |
T6069 |
auxpass |
was,recorded |
R3665 |
T6071 |
T6072 |
punct |
(,passages |
R3666 |
T6072 |
T6069 |
parataxis |
passages,recorded |
R3667 |
T6073 |
T6074 |
quantmod |
up,20 |
R3668 |
T6074 |
T6072 |
nummod |
20,passages |
R3669 |
T6075 |
T6074 |
quantmod |
to,20 |
R3670 |
T6076 |
T6074 |
punct |
~,20 |
R3671 |
T6077 |
T6072 |
punct |
),passages |
R3672 |
T6078 |
T6069 |
punct |
", ",recorded |
R3673 |
T6079 |
T6069 |
cc |
but,recorded |
R3674 |
T6080 |
T6069 |
conj |
had,recorded |
R3675 |
T6081 |
T6082 |
det |
no,impact |
R3676 |
T6082 |
T6080 |
dobj |
impact,had |
R3677 |
T6083 |
T6082 |
amod |
apparent,impact |
R3678 |
T6084 |
T6082 |
prep |
on,impact |
R3679 |
T6085 |
T6084 |
pobj |
phenotype,on |
R3680 |
T6086 |
T6069 |
punct |
.,recorded |
R3681 |
T6088 |
T6089 |
amod |
Neuronal,differentiation |
R3682 |
T6089 |
T6090 |
nsubj |
differentiation,was |
R3683 |
T6091 |
T6092 |
mark |
as,described |
R3684 |
T6092 |
T6090 |
advcl |
described,was |
R3685 |
T6093 |
T6092 |
advmod |
previously,described |
R3686 |
T6094 |
T6095 |
punct |
[,29 |
R3687 |
T6095 |
T6092 |
parataxis |
29,described |
R3688 |
T6096 |
T6095 |
punct |
],29 |
R3689 |
T6097 |
T6090 |
prep |
with,was |
R3690 |
T6098 |
T6099 |
det |
the,cocktail |
R3691 |
T6099 |
T6097 |
pobj |
cocktail,with |
R3692 |
T6100 |
T6099 |
amod |
following,cocktail |
R3693 |
T6101 |
T6099 |
punct |
: ,cocktail |
R3694 |
T6102 |
T6103 |
nummod |
10,ng |
R3695 |
T6103 |
T6104 |
nmod |
ng,FGF |
R3696 |
T6104 |
T6099 |
appos |
FGF,cocktail |
R3697 |
T6105 |
T6106 |
punct |
/,ml |
R3698 |
T6106 |
T6103 |
prep |
ml,ng |
R3699 |
T6107 |
T6104 |
compound |
α,FGF |
R3700 |
T6108 |
T6104 |
punct |
-,FGF |
R3701 |
T6109 |
T6104 |
punct |
", ",FGF |
R3702 |
T6110 |
T6111 |
nummod |
250,μM |
R3703 |
T6111 |
T6112 |
compound |
μM,IBMX |
R3704 |
T6112 |
T6104 |
appos |
IBMX,FGF |
R3705 |
T6113 |
T6104 |
punct |
", ",FGF |
R3706 |
T6114 |
T6115 |
nummod |
200,nM |
R3707 |
T6115 |
T6116 |
compound |
nM,TPA |
R3708 |
T6116 |
T6104 |
appos |
TPA,FGF |
R3709 |
T6117 |
T6104 |
punct |
", ",FGF |
R3710 |
T6118 |
T6119 |
nummod |
50,μM |
R3711 |
T6119 |
T6120 |
compound |
μM,forskolin |
R3712 |
T6120 |
T6104 |
appos |
forskolin,FGF |
R3713 |
T6121 |
T6104 |
punct |
", ",FGF |
R3714 |
T6122 |
T6123 |
nummod |
5,μM |
R3715 |
T6123 |
T6124 |
compound |
μM,dopamine |
R3716 |
T6124 |
T6104 |
appos |
dopamine,FGF |
R3717 |
T6125 |
T6126 |
punct |
(,Sigma |
R3718 |
T6126 |
T6104 |
parataxis |
Sigma,FGF |
R3719 |
T6127 |
T6126 |
punct |
),Sigma |
R3720 |
T6128 |
T6090 |
punct |
.,was |