Id |
Subject |
Object |
Predicate |
Lexical cue |
T5242 |
0-6 |
NN |
denotes |
Nestin |
T5243 |
109-119 |
NNS |
denotes |
antibodies |
T5244 |
7-8 |
-LRB- |
denotes |
( |
T5245 |
25-27 |
NN |
denotes |
μg |
T5246 |
8-13 |
NN |
denotes |
clone |
T5247 |
14-17 |
NN |
denotes |
Rat |
T5248 |
18-21 |
CD |
denotes |
401 |
T5249 |
21-23 |
, |
denotes |
, |
T5250 |
23-24 |
CD |
denotes |
2 |
T5251 |
27-28 |
SYM |
denotes |
/ |
T5252 |
28-30 |
NN |
denotes |
ml |
T5253 |
30-31 |
-RRB- |
denotes |
) |
T5254 |
31-33 |
, |
denotes |
, |
T5255 |
33-37 |
NN |
denotes |
Lamp |
T5256 |
38-39 |
CD |
denotes |
1 |
T5257 |
40-41 |
-LRB- |
denotes |
( |
T5258 |
55-57 |
NN |
denotes |
μg |
T5259 |
41-46 |
NN |
denotes |
clone |
T5260 |
47-51 |
NN |
denotes |
1D4B |
T5261 |
51-53 |
, |
denotes |
, |
T5262 |
53-54 |
CD |
denotes |
6 |
T5263 |
57-58 |
SYM |
denotes |
/ |
T5264 |
58-60 |
NN |
denotes |
ml |
T5265 |
60-61 |
-RRB- |
denotes |
) |
T5266 |
61-63 |
, |
denotes |
, |
T5267 |
63-66 |
CC |
denotes |
and |
T5268 |
67-71 |
NN |
denotes |
Lamp |
T5269 |
72-73 |
CD |
denotes |
2 |
T5270 |
74-75 |
-LRB- |
denotes |
( |
T5271 |
91-93 |
NN |
denotes |
μg |
T5272 |
75-80 |
NN |
denotes |
clone |
T5273 |
81-84 |
NN |
denotes |
Abl |
T5274 |
84-85 |
HYPH |
denotes |
- |
T5275 |
85-87 |
CD |
denotes |
93 |
T5276 |
87-88 |
: |
denotes |
; |
T5277 |
89-90 |
CD |
denotes |
6 |
T5278 |
93-94 |
SYM |
denotes |
/ |
T5279 |
94-96 |
NN |
denotes |
ml |
T5280 |
96-97 |
-RRB- |
denotes |
) |
T5281 |
98-108 |
JJ |
denotes |
monoclonal |
T5282 |
125-133 |
VBN |
denotes |
obtained |
T5283 |
120-124 |
VBD |
denotes |
were |
T5284 |
134-138 |
IN |
denotes |
from |
T5285 |
139-142 |
DT |
denotes |
the |
T5286 |
175-179 |
NNP |
denotes |
Bank |
T5287 |
143-156 |
NNP |
denotes |
Developmental |
T5288 |
157-164 |
NNPS |
denotes |
Studies |
T5289 |
165-174 |
NNP |
denotes |
Hybridoma |
T5290 |
179-181 |
, |
denotes |
, |
T5291 |
181-191 |
VBN |
denotes |
maintained |
T5292 |
192-194 |
IN |
denotes |
by |
T5293 |
195-198 |
DT |
denotes |
The |
T5294 |
199-209 |
NNP |
denotes |
University |
T5295 |
219-229 |
NNP |
denotes |
Department |
T5296 |
210-212 |
IN |
denotes |
of |
T5297 |
213-217 |
NNP |
denotes |
Iowa |
T5298 |
217-219 |
, |
denotes |
, |
T5299 |
230-232 |
IN |
denotes |
of |
T5300 |
233-243 |
NNP |
denotes |
Biological |
T5301 |
244-252 |
NNPS |
denotes |
Sciences |
T5302 |
252-253 |
. |
denotes |
. |
T5303 |
253-300 |
sentence |
denotes |
Batp1 (1 μg/ml) was previously described [12]. |
T5304 |
254-259 |
NN |
denotes |
Batp1 |
T5305 |
285-294 |
VBN |
denotes |
described |
T5306 |
260-261 |
-LRB- |
denotes |
( |
T5307 |
263-265 |
NN |
denotes |
μg |
T5308 |
261-262 |
CD |
denotes |
1 |
T5309 |
265-266 |
SYM |
denotes |
/ |
T5310 |
266-268 |
NN |
denotes |
ml |
T5311 |
268-269 |
-RRB- |
denotes |
) |
T5312 |
270-273 |
VBD |
denotes |
was |
T5313 |
274-284 |
RB |
denotes |
previously |
T5314 |
295-296 |
-LRB- |
denotes |
[ |
T5315 |
296-298 |
CD |
denotes |
12 |
T5316 |
298-299 |
-RRB- |
denotes |
] |
T5317 |
299-300 |
. |
denotes |
. |
T5318 |
300-409 |
sentence |
denotes |
Anti-subunit c antibody (0.7 μg/ml) was kindly provided by Dr. Kominami (Juntendo University, Tokyo, Japan). |
T5319 |
301-313 |
JJ |
denotes |
Anti-subunit |
T5320 |
314-315 |
NN |
denotes |
c |
T5321 |
316-324 |
NN |
denotes |
antibody |
T5322 |
348-356 |
VBN |
denotes |
provided |
T5323 |
325-326 |
-LRB- |
denotes |
( |
T5324 |
330-332 |
NN |
denotes |
μg |
T5325 |
326-329 |
CD |
denotes |
0.7 |
T5326 |
332-333 |
SYM |
denotes |
/ |
T5327 |
333-335 |
NN |
denotes |
ml |
T5328 |
335-336 |
-RRB- |
denotes |
) |
T5329 |
337-340 |
VBD |
denotes |
was |
T5330 |
341-347 |
RB |
denotes |
kindly |
T5331 |
357-359 |
IN |
denotes |
by |
T5332 |
360-363 |
NNP |
denotes |
Dr. |
T5333 |
364-372 |
NNP |
denotes |
Kominami |
T5334 |
373-374 |
-LRB- |
denotes |
( |
T5335 |
383-393 |
NNP |
denotes |
University |
T5336 |
374-382 |
NNP |
denotes |
Juntendo |
T5337 |
393-395 |
, |
denotes |
, |
T5338 |
395-400 |
NNP |
denotes |
Tokyo |
T5339 |
400-402 |
, |
denotes |
, |
T5340 |
402-407 |
NNP |
denotes |
Japan |
T5341 |
407-408 |
-RRB- |
denotes |
) |
T5342 |
408-409 |
. |
denotes |
. |
T5343 |
409-982 |
sentence |
denotes |
Additional antibodies were as follows: GFAP, 1:2000 (DAKO Corporation); calbindin, 1:5000 (Sigma); NeuN, 10 μg/ml (Chemicon); SV40 T antigen (Pab 101), 2 μg/ml (Santa Cruz Biotechnology); cathepsin D (C-20), 2 μg/ml (Santa Cruz Biotechnology); cytochrome c oxidase subunit IV (cox4), 1:1000 (BD Biosciences Clontech); PDI (H-160), 2 μg/ml (Santa Cruz Biotechnology); GM130, 1 μg/ml (BD Transduction Labs); α-tubulin, 1:15,000 (Sigma); grp75, 1:200 (Stressgen); early endosome antigen-1 (EEA1), 2 μg/ml (Santa Cruz Biotechnology); rab 7, 4 μg/ml (Santa Cruz Biotechnology). |
T5344 |
410-420 |
JJ |
denotes |
Additional |
T5345 |
421-431 |
NNS |
denotes |
antibodies |
T5346 |
432-436 |
VBD |
denotes |
were |
T5347 |
437-439 |
IN |
denotes |
as |
T5348 |
440-447 |
VBZ |
denotes |
follows |
T5349 |
447-449 |
: |
denotes |
: |
T5350 |
449-453 |
NN |
denotes |
GFAP |
T5351 |
453-455 |
, |
denotes |
, |
T5352 |
455-456 |
CD |
denotes |
1 |
T5353 |
456-457 |
SYM |
denotes |
: |
T5354 |
457-461 |
CD |
denotes |
2000 |
T5355 |
462-463 |
-LRB- |
denotes |
( |
T5356 |
468-479 |
NN |
denotes |
Corporation |
T5357 |
463-467 |
NN |
denotes |
DAKO |
T5358 |
479-480 |
-RRB- |
denotes |
) |
T5359 |
480-481 |
: |
denotes |
; |
T5360 |
482-491 |
NN |
denotes |
calbindin |
T5361 |
491-493 |
, |
denotes |
, |
T5362 |
493-494 |
CD |
denotes |
1 |
T5363 |
494-495 |
SYM |
denotes |
: |
T5364 |
495-499 |
CD |
denotes |
5000 |
T5365 |
500-501 |
-LRB- |
denotes |
( |
T5366 |
501-506 |
NNP |
denotes |
Sigma |
T5367 |
506-507 |
-RRB- |
denotes |
) |
T5368 |
507-508 |
: |
denotes |
; |
T5369 |
509-513 |
NN |
denotes |
NeuN |
T5370 |
513-515 |
, |
denotes |
, |
T5371 |
515-517 |
CD |
denotes |
10 |
T5372 |
518-520 |
NN |
denotes |
μg |
T5373 |
520-521 |
SYM |
denotes |
/ |
T5374 |
521-523 |
NN |
denotes |
ml |
T5375 |
524-525 |
-LRB- |
denotes |
( |
T5376 |
525-533 |
NNP |
denotes |
Chemicon |
T5377 |
533-534 |
-RRB- |
denotes |
) |
T5378 |
534-535 |
: |
denotes |
; |
T5379 |
536-540 |
NN |
denotes |
SV40 |
T5380 |
543-550 |
NN |
denotes |
antigen |
T5381 |
541-542 |
NN |
denotes |
T |
T5382 |
551-552 |
-LRB- |
denotes |
( |
T5383 |
552-555 |
NN |
denotes |
Pab |
T5384 |
556-559 |
CD |
denotes |
101 |
T5385 |
559-560 |
-RRB- |
denotes |
) |
T5386 |
560-562 |
, |
denotes |
, |
T5387 |
562-563 |
CD |
denotes |
2 |
T5388 |
564-566 |
NN |
denotes |
μg |
T5389 |
566-567 |
SYM |
denotes |
/ |
T5390 |
567-569 |
NN |
denotes |
ml |
T5391 |
570-571 |
-LRB- |
denotes |
( |
T5392 |
582-595 |
NNP |
denotes |
Biotechnology |
T5393 |
571-576 |
NNP |
denotes |
Santa |
T5394 |
577-581 |
NNP |
denotes |
Cruz |
T5395 |
595-596 |
-RRB- |
denotes |
) |
T5396 |
596-597 |
: |
denotes |
; |
T5397 |
598-607 |
NN |
denotes |
cathepsin |
T5398 |
608-609 |
NN |
denotes |
D |
T5399 |
610-611 |
-LRB- |
denotes |
( |
T5400 |
611-612 |
NN |
denotes |
C |
T5401 |
612-613 |
HYPH |
denotes |
- |
T5402 |
613-615 |
CD |
denotes |
20 |
T5403 |
615-616 |
-RRB- |
denotes |
) |
T5404 |
616-618 |
, |
denotes |
, |
T5405 |
618-619 |
CD |
denotes |
2 |
T5406 |
620-622 |
NN |
denotes |
μg |
T5407 |
622-623 |
SYM |
denotes |
/ |
T5408 |
623-625 |
NN |
denotes |
ml |
T5409 |
626-627 |
-LRB- |
denotes |
( |
T5410 |
638-651 |
NNP |
denotes |
Biotechnology |
T5411 |
627-632 |
NNP |
denotes |
Santa |
T5412 |
633-637 |
NNP |
denotes |
Cruz |
T5413 |
651-652 |
-RRB- |
denotes |
) |
T5414 |
652-653 |
: |
denotes |
; |
T5415 |
654-664 |
NN |
denotes |
cytochrome |
T5416 |
675-682 |
NN |
denotes |
subunit |
T5417 |
665-666 |
NN |
denotes |
c |
T5418 |
667-674 |
NN |
denotes |
oxidase |
T5419 |
683-685 |
CD |
denotes |
IV |
T5420 |
686-687 |
-LRB- |
denotes |
( |
T5421 |
687-691 |
NN |
denotes |
cox4 |
T5422 |
691-692 |
-RRB- |
denotes |
) |
T5423 |
692-694 |
, |
denotes |
, |
T5424 |
694-695 |
CD |
denotes |
1 |
T5425 |
695-696 |
SYM |
denotes |
: |
T5426 |
696-700 |
CD |
denotes |
1000 |
T5427 |
701-702 |
-LRB- |
denotes |
( |
T5428 |
717-725 |
NNP |
denotes |
Clontech |
T5429 |
702-704 |
NN |
denotes |
BD |
T5430 |
705-716 |
NNP |
denotes |
Biosciences |
T5431 |
725-726 |
-RRB- |
denotes |
) |
T5432 |
726-727 |
: |
denotes |
; |
T5433 |
728-731 |
NN |
denotes |
PDI |
T5434 |
732-733 |
-LRB- |
denotes |
( |
T5435 |
733-734 |
NN |
denotes |
H |
T5436 |
734-735 |
HYPH |
denotes |
- |
T5437 |
735-738 |
CD |
denotes |
160 |
T5438 |
738-739 |
-RRB- |
denotes |
) |
T5439 |
739-741 |
, |
denotes |
, |
T5440 |
741-742 |
CD |
denotes |
2 |
T5441 |
743-745 |
NN |
denotes |
μg |
T5442 |
745-746 |
SYM |
denotes |
/ |
T5443 |
746-748 |
NN |
denotes |
ml |
T5444 |
749-750 |
-LRB- |
denotes |
( |
T5445 |
761-774 |
NNP |
denotes |
Biotechnology |
T5446 |
750-755 |
NNP |
denotes |
Santa |
T5447 |
756-760 |
NNP |
denotes |
Cruz |
T5448 |
774-775 |
-RRB- |
denotes |
) |
T5449 |
775-776 |
: |
denotes |
; |
T5450 |
777-782 |
NN |
denotes |
GM130 |
T5451 |
782-784 |
, |
denotes |
, |
T5452 |
784-785 |
CD |
denotes |
1 |
T5453 |
786-788 |
NN |
denotes |
μg |
T5454 |
788-789 |
SYM |
denotes |
/ |
T5455 |
789-791 |
NN |
denotes |
ml |
T5456 |
792-793 |
-LRB- |
denotes |
( |
T5457 |
809-813 |
NNS |
denotes |
Labs |
T5458 |
793-795 |
NN |
denotes |
BD |
T5459 |
796-808 |
NN |
denotes |
Transduction |
T5460 |
813-814 |
-RRB- |
denotes |
) |
T5461 |
814-815 |
: |
denotes |
; |
T5462 |
816-817 |
NN |
denotes |
α |
T5463 |
818-825 |
NN |
denotes |
tubulin |
T5464 |
817-818 |
HYPH |
denotes |
- |
T5465 |
825-827 |
, |
denotes |
, |
T5466 |
827-828 |
CD |
denotes |
1 |
T5467 |
828-829 |
SYM |
denotes |
: |
T5468 |
829-835 |
CD |
denotes |
15,000 |
T5469 |
836-837 |
-LRB- |
denotes |
( |
T5470 |
837-842 |
NNP |
denotes |
Sigma |
T5471 |
842-843 |
-RRB- |
denotes |
) |
T5472 |
843-844 |
: |
denotes |
; |
T5473 |
845-850 |
NN |
denotes |
grp75 |
T5474 |
850-852 |
, |
denotes |
, |
T5475 |
852-853 |
CD |
denotes |
1 |
T5476 |
853-854 |
SYM |
denotes |
: |
T5477 |
854-857 |
CD |
denotes |
200 |
T5478 |
858-859 |
-LRB- |
denotes |
( |
T5479 |
859-868 |
NNP |
denotes |
Stressgen |
T5480 |
868-869 |
-RRB- |
denotes |
) |
T5481 |
869-870 |
: |
denotes |
; |
T5482 |
871-876 |
JJ |
denotes |
early |
T5483 |
886-893 |
NN |
denotes |
antigen |
T5484 |
877-885 |
NN |
denotes |
endosome |
T5485 |
893-894 |
HYPH |
denotes |
- |
T5486 |
894-895 |
CD |
denotes |
1 |
T5487 |
896-897 |
-LRB- |
denotes |
( |
T5488 |
897-901 |
NN |
denotes |
EEA1 |
T5489 |
901-902 |
-RRB- |
denotes |
) |
T5490 |
902-904 |
, |
denotes |
, |
T5491 |
904-905 |
CD |
denotes |
2 |
T5492 |
906-908 |
NN |
denotes |
μg |
T5493 |
908-909 |
SYM |
denotes |
/ |
T5494 |
909-911 |
NN |
denotes |
ml |
T5495 |
912-913 |
-LRB- |
denotes |
( |
T5496 |
924-937 |
NNP |
denotes |
Biotechnology |
T5497 |
913-918 |
NNP |
denotes |
Santa |
T5498 |
919-923 |
NNP |
denotes |
Cruz |
T5499 |
937-938 |
-RRB- |
denotes |
) |
T5500 |
938-939 |
: |
denotes |
; |
T5501 |
940-943 |
NN |
denotes |
rab |
T5502 |
944-945 |
CD |
denotes |
7 |
T5503 |
945-947 |
, |
denotes |
, |
T5504 |
947-948 |
CD |
denotes |
4 |
T5505 |
949-951 |
NN |
denotes |
μg |
T5506 |
951-952 |
SYM |
denotes |
/ |
T5507 |
952-954 |
NN |
denotes |
ml |
T5508 |
955-956 |
-LRB- |
denotes |
( |
T5509 |
967-980 |
NNP |
denotes |
Biotechnology |
T5510 |
956-961 |
NNP |
denotes |
Santa |
T5511 |
962-966 |
NNP |
denotes |
Cruz |
T5512 |
980-981 |
-RRB- |
denotes |
) |
T5513 |
981-982 |
. |
denotes |
. |
T5514 |
982-1155 |
sentence |
denotes |
All fluorescent secondary antibodies were obtained from Jackson ImmunoResearch Laboratories and HRP-conjugated secondary antibodies were obtained from Amersham Biosciences. |
T5515 |
983-986 |
DT |
denotes |
All |
T5516 |
1009-1019 |
NNS |
denotes |
antibodies |
T5517 |
987-998 |
JJ |
denotes |
fluorescent |
T5518 |
999-1008 |
JJ |
denotes |
secondary |
T5519 |
1025-1033 |
VBN |
denotes |
obtained |
T5520 |
1020-1024 |
VBD |
denotes |
were |
T5521 |
1034-1038 |
IN |
denotes |
from |
T5522 |
1039-1046 |
NNP |
denotes |
Jackson |
T5523 |
1062-1074 |
NNP |
denotes |
Laboratories |
T5524 |
1047-1061 |
NNP |
denotes |
ImmunoResearch |
T5525 |
1075-1078 |
CC |
denotes |
and |
T5526 |
1079-1082 |
NN |
denotes |
HRP |
T5527 |
1083-1093 |
VBN |
denotes |
conjugated |
T5528 |
1082-1083 |
HYPH |
denotes |
- |
T5529 |
1104-1114 |
NNS |
denotes |
antibodies |
T5530 |
1094-1103 |
JJ |
denotes |
secondary |
T5531 |
1120-1128 |
VBN |
denotes |
obtained |
T5532 |
1115-1119 |
VBD |
denotes |
were |
T5533 |
1129-1133 |
IN |
denotes |
from |
T5534 |
1134-1142 |
NNP |
denotes |
Amersham |
T5535 |
1143-1154 |
NNP |
denotes |
Biosciences |
T5536 |
1154-1155 |
. |
denotes |
. |
T5537 |
1155-1325 |
sentence |
denotes |
Additional cell markers were as follows: VVL-biotin, 1:2000 (Vector Laboratories), 10,000 MW dextran-FITC, 1 mg/ml and Lysotracker Red DND-99, 500 nM (Molecular Probes). |
T5538 |
1156-1166 |
JJ |
denotes |
Additional |
T5539 |
1172-1179 |
NNS |
denotes |
markers |
T5540 |
1167-1171 |
NN |
denotes |
cell |
T5541 |
1180-1184 |
VBD |
denotes |
were |
T5542 |
1185-1187 |
IN |
denotes |
as |
T5543 |
1188-1195 |
VBZ |
denotes |
follows |
T5544 |
1195-1197 |
: |
denotes |
: |
T5545 |
1197-1200 |
NN |
denotes |
VVL |
T5546 |
1201-1207 |
NN |
denotes |
biotin |
T5547 |
1200-1201 |
HYPH |
denotes |
- |
T5548 |
1207-1209 |
, |
denotes |
, |
T5549 |
1209-1210 |
CD |
denotes |
1 |
T5550 |
1210-1211 |
SYM |
denotes |
: |
T5551 |
1211-1215 |
CD |
denotes |
2000 |
T5552 |
1216-1217 |
-LRB- |
denotes |
( |
T5553 |
1224-1236 |
NNP |
denotes |
Laboratories |
T5554 |
1217-1223 |
NNP |
denotes |
Vector |
T5555 |
1236-1237 |
-RRB- |
denotes |
) |
T5556 |
1237-1239 |
, |
denotes |
, |
T5557 |
1239-1245 |
CD |
denotes |
10,000 |
T5558 |
1246-1248 |
NN |
denotes |
MW |
T5559 |
1257-1261 |
NN |
denotes |
FITC |
T5560 |
1249-1256 |
NN |
denotes |
dextran |
T5561 |
1256-1257 |
HYPH |
denotes |
- |
T5562 |
1261-1263 |
, |
denotes |
, |
T5563 |
1263-1264 |
CD |
denotes |
1 |
T5564 |
1265-1267 |
NN |
denotes |
mg |
T5565 |
1267-1268 |
SYM |
denotes |
/ |
T5566 |
1268-1270 |
NN |
denotes |
ml |
T5567 |
1271-1274 |
CC |
denotes |
and |
T5568 |
1275-1286 |
NNP |
denotes |
Lysotracker |
T5569 |
1291-1294 |
NN |
denotes |
DND |
T5570 |
1287-1290 |
NN |
denotes |
Red |
T5571 |
1294-1295 |
HYPH |
denotes |
- |
T5572 |
1295-1297 |
CD |
denotes |
99 |
T5573 |
1297-1299 |
, |
denotes |
, |
T5574 |
1299-1302 |
CD |
denotes |
500 |
T5575 |
1303-1305 |
NN |
denotes |
nM |
T5576 |
1306-1307 |
-LRB- |
denotes |
( |
T5577 |
1317-1323 |
NNP |
denotes |
Probes |
T5578 |
1307-1316 |
NNP |
denotes |
Molecular |
T5579 |
1323-1324 |
-RRB- |
denotes |
) |
T5580 |
1324-1325 |
. |
denotes |
. |
R3004 |
T5242 |
T5243 |
nmod |
Nestin,antibodies |
R3005 |
T5243 |
T5282 |
nsubjpass |
antibodies,obtained |
R3006 |
T5244 |
T5245 |
punct |
(,μg |
R3007 |
T5245 |
T5242 |
parataxis |
μg,Nestin |
R3008 |
T5246 |
T5247 |
compound |
clone,Rat |
R3009 |
T5247 |
T5245 |
dep |
Rat,μg |
R3010 |
T5248 |
T5247 |
nummod |
401,Rat |
R3011 |
T5249 |
T5245 |
punct |
", ",μg |
R3012 |
T5250 |
T5245 |
nummod |
2,μg |
R3013 |
T5251 |
T5252 |
punct |
/,ml |
R3014 |
T5252 |
T5245 |
prep |
ml,μg |
R3015 |
T5253 |
T5245 |
punct |
),μg |
R3016 |
T5254 |
T5242 |
punct |
", ",Nestin |
R3017 |
T5255 |
T5242 |
conj |
Lamp,Nestin |
R3018 |
T5256 |
T5255 |
nummod |
1,Lamp |
R3019 |
T5257 |
T5258 |
punct |
(,μg |
R3020 |
T5258 |
T5255 |
parataxis |
μg,Lamp |
R3021 |
T5259 |
T5260 |
compound |
clone,1D4B |
R3022 |
T5260 |
T5258 |
dep |
1D4B,μg |
R3023 |
T5261 |
T5258 |
punct |
", ",μg |
R3024 |
T5262 |
T5258 |
nummod |
6,μg |
R3025 |
T5263 |
T5264 |
punct |
/,ml |
R3026 |
T5264 |
T5258 |
prep |
ml,μg |
R3027 |
T5265 |
T5258 |
punct |
),μg |
R3028 |
T5266 |
T5255 |
punct |
", ",Lamp |
R3029 |
T5267 |
T5255 |
cc |
and,Lamp |
R3030 |
T5268 |
T5255 |
conj |
Lamp,Lamp |
R3031 |
T5269 |
T5268 |
nummod |
2,Lamp |
R3032 |
T5270 |
T5271 |
punct |
(,μg |
R3033 |
T5271 |
T5268 |
parataxis |
μg,Lamp |
R3034 |
T5272 |
T5273 |
compound |
clone,Abl |
R3035 |
T5273 |
T5271 |
dep |
Abl,μg |
R3036 |
T5274 |
T5273 |
punct |
-,Abl |
R3037 |
T5275 |
T5273 |
nummod |
93,Abl |
R3038 |
T5276 |
T5271 |
punct |
;,μg |
R3039 |
T5277 |
T5271 |
nummod |
6,μg |
R3040 |
T5278 |
T5279 |
punct |
/,ml |
R3041 |
T5279 |
T5271 |
prep |
ml,μg |
R3042 |
T5280 |
T5271 |
punct |
),μg |
R3043 |
T5281 |
T5243 |
amod |
monoclonal,antibodies |
R3044 |
T5283 |
T5282 |
auxpass |
were,obtained |
R3045 |
T5284 |
T5282 |
prep |
from,obtained |
R3046 |
T5285 |
T5286 |
det |
the,Bank |
R3047 |
T5286 |
T5284 |
pobj |
Bank,from |
R3048 |
T5287 |
T5286 |
compound |
Developmental,Bank |
R3049 |
T5288 |
T5286 |
compound |
Studies,Bank |
R3050 |
T5289 |
T5286 |
compound |
Hybridoma,Bank |
R3051 |
T5290 |
T5286 |
punct |
", ",Bank |
R3052 |
T5291 |
T5286 |
acl |
maintained,Bank |
R3053 |
T5292 |
T5291 |
agent |
by,maintained |
R3054 |
T5293 |
T5294 |
det |
The,University |
R3055 |
T5294 |
T5295 |
nmod |
University,Department |
R3056 |
T5295 |
T5292 |
pobj |
Department,by |
R3057 |
T5296 |
T5294 |
prep |
of,University |
R3058 |
T5297 |
T5296 |
pobj |
Iowa,of |
R3059 |
T5298 |
T5295 |
punct |
", ",Department |
R3060 |
T5299 |
T5295 |
prep |
of,Department |
R3061 |
T5300 |
T5301 |
compound |
Biological,Sciences |
R3062 |
T5301 |
T5299 |
pobj |
Sciences,of |
R3063 |
T5302 |
T5282 |
punct |
.,obtained |
R3064 |
T5304 |
T5305 |
nsubjpass |
Batp1,described |
R3065 |
T5306 |
T5307 |
punct |
(,μg |
R3066 |
T5307 |
T5304 |
parataxis |
μg,Batp1 |
R3067 |
T5308 |
T5307 |
nummod |
1,μg |
R3068 |
T5309 |
T5310 |
punct |
/,ml |
R3069 |
T5310 |
T5307 |
prep |
ml,μg |
R3070 |
T5311 |
T5307 |
punct |
),μg |
R3071 |
T5312 |
T5305 |
auxpass |
was,described |
R3072 |
T5313 |
T5305 |
advmod |
previously,described |
R3073 |
T5314 |
T5315 |
punct |
[,12 |
R3074 |
T5315 |
T5305 |
parataxis |
12,described |
R3075 |
T5316 |
T5315 |
punct |
],12 |
R3076 |
T5317 |
T5305 |
punct |
.,described |
R3077 |
T5319 |
T5320 |
amod |
Anti-subunit,c |
R3078 |
T5320 |
T5321 |
amod |
c,antibody |
R3079 |
T5321 |
T5322 |
nsubjpass |
antibody,provided |
R3080 |
T5323 |
T5324 |
punct |
(,μg |
R3081 |
T5324 |
T5321 |
parataxis |
μg,antibody |
R3082 |
T5325 |
T5324 |
nummod |
0.7,μg |
R3083 |
T5326 |
T5327 |
punct |
/,ml |
R3084 |
T5327 |
T5324 |
prep |
ml,μg |
R3085 |
T5328 |
T5324 |
punct |
),μg |
R3086 |
T5329 |
T5322 |
auxpass |
was,provided |
R3087 |
T5330 |
T5322 |
advmod |
kindly,provided |
R3088 |
T5331 |
T5322 |
agent |
by,provided |
R3089 |
T5332 |
T5333 |
compound |
Dr.,Kominami |
R3090 |
T5333 |
T5331 |
pobj |
Kominami,by |
R3091 |
T5334 |
T5335 |
punct |
(,University |
R3092 |
T5335 |
T5333 |
parataxis |
University,Kominami |
R3093 |
T5336 |
T5335 |
compound |
Juntendo,University |
R3094 |
T5337 |
T5335 |
punct |
", ",University |
R3095 |
T5338 |
T5335 |
npadvmod |
Tokyo,University |
R3096 |
T5339 |
T5335 |
punct |
", ",University |
R3097 |
T5340 |
T5335 |
npadvmod |
Japan,University |
R3098 |
T5341 |
T5335 |
punct |
),University |
R3099 |
T5342 |
T5322 |
punct |
.,provided |
R3100 |
T5344 |
T5345 |
amod |
Additional,antibodies |
R3101 |
T5345 |
T5346 |
nsubj |
antibodies,were |
R3102 |
T5347 |
T5348 |
mark |
as,follows |
R3103 |
T5348 |
T5346 |
advcl |
follows,were |
R3104 |
T5349 |
T5346 |
punct |
: ,were |
R3105 |
T5350 |
T5346 |
dep |
GFAP,were |
R3106 |
T5351 |
T5350 |
punct |
", ",GFAP |
R3107 |
T5352 |
T5350 |
npadvmod |
1,GFAP |
R3108 |
T5353 |
T5354 |
punct |
:,2000 |
R3109 |
T5354 |
T5352 |
prep |
2000,1 |
R3110 |
T5355 |
T5356 |
punct |
(,Corporation |
R3111 |
T5356 |
T5350 |
parataxis |
Corporation,GFAP |
R3112 |
T5357 |
T5356 |
compound |
DAKO,Corporation |
R3113 |
T5358 |
T5356 |
punct |
),Corporation |
R3114 |
T5359 |
T5350 |
punct |
;,GFAP |
R3115 |
T5360 |
T5350 |
appos |
calbindin,GFAP |
R3116 |
T5361 |
T5360 |
punct |
", ",calbindin |
R3117 |
T5362 |
T5360 |
npadvmod |
1,calbindin |
R3118 |
T5363 |
T5364 |
punct |
:,5000 |
R3119 |
T5364 |
T5362 |
prep |
5000,1 |
R3120 |
T5365 |
T5366 |
punct |
(,Sigma |
R3121 |
T5366 |
T5360 |
parataxis |
Sigma,calbindin |
R3122 |
T5367 |
T5366 |
punct |
),Sigma |
R3123 |
T5368 |
T5350 |
punct |
;,GFAP |
R3124 |
T5369 |
T5350 |
appos |
NeuN,GFAP |
R3125 |
T5370 |
T5369 |
punct |
", ",NeuN |
R3126 |
T5371 |
T5372 |
nummod |
10,μg |
R3127 |
T5372 |
T5369 |
npadvmod |
μg,NeuN |
R3128 |
T5373 |
T5374 |
punct |
/,ml |
R3129 |
T5374 |
T5372 |
prep |
ml,μg |
R3130 |
T5375 |
T5376 |
punct |
(,Chemicon |
R3131 |
T5376 |
T5369 |
parataxis |
Chemicon,NeuN |
R3132 |
T5377 |
T5376 |
punct |
),Chemicon |
R3133 |
T5378 |
T5350 |
punct |
;,GFAP |
R3134 |
T5379 |
T5380 |
compound |
SV40,antigen |
R3135 |
T5380 |
T5350 |
appos |
antigen,GFAP |
R3136 |
T5381 |
T5380 |
compound |
T,antigen |
R3137 |
T5382 |
T5380 |
punct |
(,antigen |
R3138 |
T5383 |
T5380 |
appos |
Pab,antigen |
R3139 |
T5384 |
T5383 |
nummod |
101,Pab |
R3140 |
T5385 |
T5380 |
punct |
),antigen |
R3141 |
T5386 |
T5380 |
punct |
", ",antigen |
R3142 |
T5387 |
T5388 |
nummod |
2,μg |
R3143 |
T5388 |
T5380 |
npadvmod |
μg,antigen |
R3144 |
T5389 |
T5390 |
punct |
/,ml |
R3145 |
T5390 |
T5388 |
prep |
ml,μg |
R3146 |
T5391 |
T5392 |
punct |
(,Biotechnology |
R3147 |
T5392 |
T5380 |
parataxis |
Biotechnology,antigen |
R3148 |
T5393 |
T5392 |
compound |
Santa,Biotechnology |
R3149 |
T5394 |
T5392 |
compound |
Cruz,Biotechnology |
R3150 |
T5395 |
T5392 |
punct |
),Biotechnology |
R3151 |
T5396 |
T5350 |
punct |
;,GFAP |
R3152 |
T5397 |
T5398 |
compound |
cathepsin,D |
R3153 |
T5398 |
T5350 |
appos |
D,GFAP |
R3154 |
T5399 |
T5398 |
punct |
(,D |
R3155 |
T5400 |
T5398 |
appos |
C,D |
R3156 |
T5401 |
T5400 |
punct |
-,C |
R3157 |
T5402 |
T5400 |
nummod |
20,C |
R3158 |
T5403 |
T5398 |
punct |
),D |
R3159 |
T5404 |
T5398 |
punct |
", ",D |
R3160 |
T5405 |
T5406 |
nummod |
2,μg |
R3161 |
T5406 |
T5398 |
npadvmod |
μg,D |
R3162 |
T5407 |
T5408 |
punct |
/,ml |
R3163 |
T5408 |
T5406 |
prep |
ml,μg |
R3164 |
T5409 |
T5410 |
punct |
(,Biotechnology |
R3165 |
T5410 |
T5398 |
parataxis |
Biotechnology,D |
R3166 |
T5411 |
T5410 |
compound |
Santa,Biotechnology |
R3167 |
T5412 |
T5410 |
compound |
Cruz,Biotechnology |
R3168 |
T5413 |
T5410 |
punct |
),Biotechnology |
R3169 |
T5414 |
T5350 |
punct |
;,GFAP |
R3170 |
T5415 |
T5416 |
compound |
cytochrome,subunit |
R3171 |
T5416 |
T5350 |
appos |
subunit,GFAP |
R3172 |
T5417 |
T5416 |
compound |
c,subunit |
R3173 |
T5418 |
T5416 |
compound |
oxidase,subunit |
R3174 |
T5419 |
T5416 |
nummod |
IV,subunit |
R3175 |
T5420 |
T5416 |
punct |
(,subunit |
R3176 |
T5421 |
T5416 |
appos |
cox4,subunit |
R3177 |
T5422 |
T5416 |
punct |
),subunit |
R3178 |
T5423 |
T5416 |
punct |
", ",subunit |
R3179 |
T5424 |
T5416 |
npadvmod |
1,subunit |
R3180 |
T5425 |
T5426 |
punct |
:,1000 |
R3181 |
T5426 |
T5424 |
prep |
1000,1 |
R3182 |
T5427 |
T5428 |
punct |
(,Clontech |
R3183 |
T5428 |
T5416 |
parataxis |
Clontech,subunit |
R3184 |
T5429 |
T5428 |
compound |
BD,Clontech |
R3185 |
T5430 |
T5428 |
compound |
Biosciences,Clontech |
R3186 |
T5431 |
T5428 |
punct |
),Clontech |
R3187 |
T5432 |
T5350 |
punct |
;,GFAP |
R3188 |
T5433 |
T5350 |
appos |
PDI,GFAP |
R3189 |
T5434 |
T5433 |
punct |
(,PDI |
R3190 |
T5435 |
T5433 |
appos |
H,PDI |
R3191 |
T5436 |
T5435 |
punct |
-,H |
R3192 |
T5437 |
T5435 |
nummod |
160,H |
R3193 |
T5438 |
T5433 |
punct |
),PDI |
R3194 |
T5439 |
T5433 |
punct |
", ",PDI |
R3195 |
T5440 |
T5441 |
nummod |
2,μg |
R3196 |
T5441 |
T5433 |
npadvmod |
μg,PDI |
R3197 |
T5442 |
T5443 |
punct |
/,ml |
R3198 |
T5443 |
T5441 |
prep |
ml,μg |
R3199 |
T5444 |
T5445 |
punct |
(,Biotechnology |
R3200 |
T5445 |
T5433 |
parataxis |
Biotechnology,PDI |
R3201 |
T5446 |
T5445 |
compound |
Santa,Biotechnology |
R3202 |
T5447 |
T5445 |
compound |
Cruz,Biotechnology |
R3203 |
T5448 |
T5445 |
punct |
),Biotechnology |
R3204 |
T5449 |
T5350 |
punct |
;,GFAP |
R3205 |
T5450 |
T5350 |
appos |
GM130,GFAP |
R3206 |
T5451 |
T5450 |
punct |
", ",GM130 |
R3207 |
T5452 |
T5453 |
nummod |
1,μg |
R3208 |
T5453 |
T5450 |
npadvmod |
μg,GM130 |
R3209 |
T5454 |
T5455 |
punct |
/,ml |
R3210 |
T5455 |
T5453 |
prep |
ml,μg |
R3211 |
T5456 |
T5457 |
punct |
(,Labs |
R3212 |
T5457 |
T5450 |
parataxis |
Labs,GM130 |
R3213 |
T5458 |
T5457 |
compound |
BD,Labs |
R3214 |
T5459 |
T5457 |
compound |
Transduction,Labs |
R3215 |
T5460 |
T5457 |
punct |
),Labs |
R3216 |
T5461 |
T5350 |
punct |
;,GFAP |
R3217 |
T5462 |
T5463 |
compound |
α,tubulin |
R3218 |
T5463 |
T5350 |
appos |
tubulin,GFAP |
R3219 |
T5464 |
T5463 |
punct |
-,tubulin |
R3220 |
T5465 |
T5463 |
punct |
", ",tubulin |
R3221 |
T5466 |
T5463 |
npadvmod |
1,tubulin |
R3222 |
T5467 |
T5468 |
punct |
:,"15,000" |
R3223 |
T5468 |
T5466 |
prep |
"15,000",1 |
R3224 |
T5469 |
T5470 |
punct |
(,Sigma |
R3225 |
T5470 |
T5463 |
parataxis |
Sigma,tubulin |
R3226 |
T5471 |
T5470 |
punct |
),Sigma |
R3227 |
T5472 |
T5350 |
punct |
;,GFAP |
R3228 |
T5473 |
T5350 |
appos |
grp75,GFAP |
R3229 |
T5474 |
T5473 |
punct |
", ",grp75 |
R3230 |
T5475 |
T5473 |
npadvmod |
1,grp75 |
R3231 |
T5476 |
T5477 |
punct |
:,200 |
R3232 |
T5477 |
T5475 |
prep |
200,1 |
R3233 |
T5478 |
T5479 |
punct |
(,Stressgen |
R3234 |
T5479 |
T5473 |
parataxis |
Stressgen,grp75 |
R3235 |
T5480 |
T5479 |
punct |
),Stressgen |
R3236 |
T5481 |
T5350 |
punct |
;,GFAP |
R3237 |
T5482 |
T5483 |
amod |
early,antigen |
R3238 |
T5483 |
T5350 |
appos |
antigen,GFAP |
R3239 |
T5484 |
T5483 |
compound |
endosome,antigen |
R3240 |
T5485 |
T5483 |
punct |
-,antigen |
R3241 |
T5486 |
T5483 |
nummod |
1,antigen |
R3242 |
T5487 |
T5483 |
punct |
(,antigen |
R3243 |
T5488 |
T5483 |
appos |
EEA1,antigen |
R3244 |
T5489 |
T5483 |
punct |
),antigen |
R3245 |
T5490 |
T5483 |
punct |
", ",antigen |
R3246 |
T5491 |
T5492 |
nummod |
2,μg |
R3247 |
T5492 |
T5483 |
npadvmod |
μg,antigen |
R3248 |
T5493 |
T5494 |
punct |
/,ml |
R3249 |
T5494 |
T5492 |
prep |
ml,μg |
R3250 |
T5495 |
T5496 |
punct |
(,Biotechnology |
R3251 |
T5496 |
T5483 |
parataxis |
Biotechnology,antigen |
R3252 |
T5497 |
T5496 |
compound |
Santa,Biotechnology |
R3253 |
T5498 |
T5496 |
compound |
Cruz,Biotechnology |
R3254 |
T5499 |
T5496 |
punct |
),Biotechnology |
R3255 |
T5500 |
T5350 |
punct |
;,GFAP |
R3256 |
T5501 |
T5350 |
appos |
rab,GFAP |
R3257 |
T5502 |
T5501 |
nummod |
7,rab |
R3258 |
T5503 |
T5501 |
punct |
", ",rab |
R3259 |
T5504 |
T5505 |
nummod |
4,μg |
R3260 |
T5505 |
T5501 |
npadvmod |
μg,rab |
R3261 |
T5506 |
T5507 |
punct |
/,ml |
R3262 |
T5507 |
T5505 |
prep |
ml,μg |
R3263 |
T5508 |
T5509 |
punct |
(,Biotechnology |
R3264 |
T5509 |
T5501 |
parataxis |
Biotechnology,rab |
R3265 |
T5510 |
T5509 |
compound |
Santa,Biotechnology |
R3266 |
T5511 |
T5509 |
compound |
Cruz,Biotechnology |
R3267 |
T5512 |
T5509 |
punct |
),Biotechnology |
R3268 |
T5513 |
T5346 |
punct |
.,were |
R3269 |
T5515 |
T5516 |
det |
All,antibodies |
R3270 |
T5516 |
T5519 |
nsubjpass |
antibodies,obtained |
R3271 |
T5517 |
T5516 |
amod |
fluorescent,antibodies |
R3272 |
T5518 |
T5516 |
amod |
secondary,antibodies |
R3273 |
T5520 |
T5519 |
auxpass |
were,obtained |
R3274 |
T5521 |
T5519 |
prep |
from,obtained |
R3275 |
T5522 |
T5523 |
compound |
Jackson,Laboratories |
R3276 |
T5523 |
T5521 |
pobj |
Laboratories,from |
R3277 |
T5524 |
T5523 |
compound |
ImmunoResearch,Laboratories |
R3278 |
T5525 |
T5519 |
cc |
and,obtained |
R3279 |
T5526 |
T5527 |
npadvmod |
HRP,conjugated |
R3280 |
T5527 |
T5529 |
amod |
conjugated,antibodies |
R3281 |
T5528 |
T5527 |
punct |
-,conjugated |
R3282 |
T5529 |
T5531 |
nsubjpass |
antibodies,obtained |
R3283 |
T5530 |
T5529 |
amod |
secondary,antibodies |
R3284 |
T5531 |
T5519 |
conj |
obtained,obtained |
R3285 |
T5532 |
T5531 |
auxpass |
were,obtained |
R3286 |
T5533 |
T5531 |
prep |
from,obtained |
R3287 |
T5534 |
T5535 |
compound |
Amersham,Biosciences |
R3288 |
T5535 |
T5533 |
pobj |
Biosciences,from |
R3289 |
T5536 |
T5531 |
punct |
.,obtained |
R3290 |
T5538 |
T5539 |
amod |
Additional,markers |
R3291 |
T5539 |
T5541 |
nsubj |
markers,were |
R3292 |
T5540 |
T5539 |
compound |
cell,markers |
R3293 |
T5542 |
T5543 |
mark |
as,follows |
R3294 |
T5543 |
T5541 |
advcl |
follows,were |
R3295 |
T5544 |
T5541 |
punct |
: ,were |
R3296 |
T5545 |
T5546 |
compound |
VVL,biotin |
R3297 |
T5546 |
T5541 |
dep |
biotin,were |
R3298 |
T5547 |
T5546 |
punct |
-,biotin |
R3299 |
T5548 |
T5546 |
punct |
", ",biotin |
R3300 |
T5549 |
T5546 |
npadvmod |
1,biotin |
R3301 |
T5550 |
T5551 |
punct |
:,2000 |
R3302 |
T5551 |
T5549 |
prep |
2000,1 |
R3303 |
T5552 |
T5553 |
punct |
(,Laboratories |
R3304 |
T5553 |
T5546 |
parataxis |
Laboratories,biotin |
R3305 |
T5554 |
T5553 |
compound |
Vector,Laboratories |
R3306 |
T5555 |
T5553 |
punct |
),Laboratories |
R3307 |
T5556 |
T5546 |
punct |
", ",biotin |
R3308 |
T5557 |
T5558 |
nummod |
"10,000",MW |
R3309 |
T5558 |
T5559 |
compound |
MW,FITC |
R3310 |
T5559 |
T5546 |
conj |
FITC,biotin |
R3311 |
T5560 |
T5559 |
compound |
dextran,FITC |
R3312 |
T5561 |
T5559 |
punct |
-,FITC |
R3313 |
T5562 |
T5559 |
punct |
", ",FITC |
R3314 |
T5563 |
T5564 |
nummod |
1,mg |
R3315 |
T5564 |
T5559 |
npadvmod |
mg,FITC |
R3316 |
T5565 |
T5566 |
punct |
/,ml |
R3317 |
T5566 |
T5564 |
prep |
ml,mg |
R3318 |
T5567 |
T5559 |
cc |
and,FITC |
R3319 |
T5568 |
T5569 |
compound |
Lysotracker,DND |
R3320 |
T5569 |
T5559 |
conj |
DND,FITC |
R3321 |
T5570 |
T5569 |
compound |
Red,DND |
R3322 |
T5571 |
T5569 |
punct |
-,DND |
R3323 |
T5572 |
T5569 |
nummod |
99,DND |
R3324 |
T5573 |
T5569 |
punct |
", ",DND |
R3325 |
T5574 |
T5575 |
nummod |
500,nM |
R3326 |
T5575 |
T5569 |
npadvmod |
nM,DND |
R3327 |
T5576 |
T5577 |
punct |
(,Probes |
R3328 |
T5577 |
T5569 |
parataxis |
Probes,DND |
R3329 |
T5578 |
T5577 |
compound |
Molecular,Probes |
R3330 |
T5579 |
T5577 |
punct |
),Probes |
R3331 |
T5580 |
T5541 |
punct |
.,were |