PMC:539297 / 25812-36167 JSON TXT

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craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T6601	5194-5199	GO:0040007	denotes	grown
T6602	5277-5289	CHEBI:16842	denotes	formaldehyde
T6603	5339-5347	CHEBI:17790	denotes	methanol
T6604	5348-5355	CHEBI:15347	denotes	acetone
T6605	5410-5418	GO:0042571	denotes	antibody
T6606	5610-5618	GO:0042571	denotes	antibody
T6607	5630-5642	CHEBI:9750	denotes	Triton X-100
T6608	5688-5694	NCBITaxon:27592	denotes	bovine
T6609	5695-5700	UBERON:0001977	denotes	serum
T6610	5695-5708	PR:000003918	denotes	serum albumin
T6611	5746-5754	GO:0042571	denotes	antibody
T6612	5803-5811	GO:0042571	denotes	antibody
T6613	6371-6375	CHEBI:9754	denotes	Tris
T6614	6388-6400	CHEBI:9750	denotes	Triton X-100
T6615	6402-6410	GO:0016020	denotes	membrane
T6616	6493-6504	CHEBI:7989	denotes	pepstatin A
T6617	6533-6542	CHEBI:6426	denotes	leupeptin
T6618	6787-6790	CHEBI:8984	denotes	SDS
T6619	6853-6857	CHEBI:9754	denotes	tris
T6620	6858-6865	CHEBI:46760	denotes	tricine
T6621	6866-6869	CHEBI:8984	denotes	SDS
T6622	6894-6903	PR:000022190	denotes	subunit c
T5155	9-17	GO:0042571	denotes	Antibody
T5156	36-44	CHEBI:33893	denotes	reagents
T5157	45-51	PR:000011141	denotes	Nestin
T5158	59-62	NCBITaxon:10114	denotes	Rat
T5159	78-84	PR:000002060	denotes	Lamp 1
T5160	112-118	PR:000002061	denotes	Lamp 2
T5161	154-164	GO:0042571	denotes	antibodies
T5162	351-360	PR:000022190	denotes	subunit c
T5163	361-369	GO:0042571	denotes	antibody
T5164	466-476	GO:0042571	denotes	antibodies
T5165	494-498	PR:000007939	denotes	GFAP
T5166	527-536	PR:000004967	denotes	calbindin
T5167	554-558	PR:000006252	denotes	NeuN
T5168	581-585	NCBITaxon:10633	denotes	SV40
T5169	588-595	CHEBI:59132	denotes	antigen
T5170	643-654	PR:000006025	denotes	cathepsin D
T5171	699-711	CHEBI:18070	denotes	cytochrome c
T5172	699-730	PR:000005776	denotes	cytochrome c oxidase subunit IV
T5173	732-736	PR:000005776	denotes	cox4
T5174	822-827	PR:000008131	denotes	GM130
T5175	841-853	GO:0009293	denotes	Transduction
T5176	863-870	PR:000028799	denotes	tubulin
T5177	890-895	PR:000008813	denotes	grp75
T5178	916-930	GO:0005769	denotes	early endosome
T5179	916-940	PR:000006901	denotes	early endosome antigen-1
T5180	931-938	CHEBI:59132	denotes	antigen
T5181	942-946	PR:000006901	denotes	EEA1
T5182	985-990	PR:000013639	denotes	rab 7
T5183	1054-1064	GO:0042571	denotes	antibodies
T5184	1128-1138	MOP:0000779	denotes	conjugated
T5185	1149-1159	GO:0042571	denotes	antibodies
T5186	1246-1252	CHEBI:15956	denotes	biotin
T5187	1294-1301	CHEBI:52071	denotes	dextran
T5188	1302-1306	CHEBI:37926	denotes	FITC
T5189	1320-1342	CHEBI:52117	denotes	Lysotracker Red DND-99
T5620	1419-1421	UBERON:0002037	denotes	Cb
T5621	1421-1425	PR:000005591	denotes	Cln3
T5622	1426-1436	UBERON:0002037	denotes	cerebellar
T5623	1426-1445	CL:1001611	denotes	cerebellar neuronal
T5624	1462-1466	PR:000005591	denotes	Cln3
T5625	1482-1486	NCBITaxon:10088	denotes	mice
T5626	1654-1664	UBERON:0002037	denotes	cerebellar
T5627	1654-1679	CL:0001031	denotes	cerebellar granule neuron
T5628	1708-1713	GO:0007567	denotes	natal
T5629	1725-1734	UBERON:0002037	denotes	cerebella
T5630	1822-1829	CHEBI:17234	denotes	glucose
T5631	1831-1835	UBERON:0002415	denotes	Tail
T5632	1869-1876	SO:0001026	denotes	genomic
T5633	1915-1922	PR:000027795	denotes	Trypsin
T5634	2015-2024	UBERON:0002037	denotes	cerebella
T5635	2142-2151	UBERON:0002037	denotes	cerebella
T5636	2188-2191	CHEBI:16526	denotes	CO2
T5637	2196-2210	CL:0000120	denotes	granule neuron
T5638	2298-2304	NCBITaxon:27592	denotes	bovine
T5639	2305-2310	UBERON:0001977	denotes	serum
T5640	2352-2355	CHEBI:32588	denotes	KCl
T5641	2415-2425	NCBITaxon:11632	denotes	retrovirus
T5642	2426-2437	GO:0009293	denotes	transducing
T5643	2482-2489	CHEBI:59132	denotes	antigen
T5644	2506-2514	CHEBI:7507	denotes	neomycin
T5645	2526-2534	SO:0005853	denotes	cassette
T5646	2737-2741	CHEBI:42768	denotes	G418
T5647	2996-2998	UBERON:0002037	denotes	Cb
T5648	2998-3002	PR:000005591	denotes	Cln3
T5649	3015-3017	UBERON:0002037	denotes	Cb
T5650	3124-3127	CHEBI:16526	denotes	CO2
T5651	3231-3239	CL:0000540	denotes	Neuronal
T5652	3308-3316	CHEBI:60004	denotes	cocktail
T5653	3327-3332	PR:000007479	denotes	α-FGF
T5654	3341-3345	CHEBI:48518	denotes	IBMX
T5655	3354-3357	CHEBI:37537	denotes	TPA
T5656	3365-3374	CHEBI:42471	denotes	forskolin
T5657	3381-3389	CHEBI:18243	denotes	dopamine
T6145	3699-3706	SO:0000112	denotes	primers
T6146	3800-3802	SO:0000028	denotes	bp
T6147	3952-3956	PR:000005591	denotes	Cln3
T6148	3964-3971	SO:0000112	denotes	primers
T6342	4025-4034	PR:000022190	denotes	Subunit c
T6343	4439-4451	CHEBI:16842	denotes	formaldehyde
T6344	4546-4555	PR:000022190	denotes	subunit c
T6345	4675-4689	CHEBI:64276	denotes	glutaraldehyde
T6346	4719-4729	CHEBI:16223	denotes	cacodylate
T6347	5023-5031	GO:0042571	denotes	antibody
T7096	6933-6944	PR:000006025	denotes	Cathepsin D
T7097	6967-6973	UBERON:0000479	denotes	tissue
T7098	7142-7146	CHEBI:9754	denotes	Tris
T7099	7161-7173	CHEBI:9750	denotes	Triton X-100
T7100	7435-7446	PR:000006025	denotes	cathepsin D
T7101	7466-7477	CHEBI:76219	denotes	Fluorogenic
T7102	7488-7499	PR:000006025	denotes	Cathepsin D
T7316	7875-7884	GO:0005764	denotes	Lysosomal
T7317	7898-7914	GO:0006897	denotes	endocytic uptake
T7318	8003-8008	GO:0040007	denotes	grown
T7319	8131-8138	CHEBI:52071	denotes	dextran
T7320	8139-8143	CHEBI:37926	denotes	FITC
T7321	8317-8320	CHEBI:37958	denotes	dye
T7322	8351-8363	CHEBI:16842	denotes	formaldehyde
T7476	8558-8570	GO:0005739	denotes	mitochondria
T7477	8714-8726	GO:0005739	denotes	mitochondria
T7478	8784-8796	GO:0005739	denotes	mitochondria
T7479	9060-9073	GO:0005739	denotes	mitochondrial
T7609	9521-9528	CHEBI:33893	denotes	Reagent
T7610	9556-9566	GO:0019835	denotes	cell lysis
T7768	9865-9882	CHEBI:16240	denotes	Hydrogen peroxide
T7769	10070-10087	CHEBI:16240	denotes	hydrogen peroxide
T7770	10156-10173	CHEBI:16240	denotes	hydrogen peroxide
T7771	10272-10290	GO:0008283	denotes	Cell Proliferation
T6139	3422-3429	SO:0001026	denotes	Genomic
T6140	3453-3457	UBERON:0002415	denotes	tail
T6141	3520-3524	PR:000005591	denotes	Cln3
T6142	3540-3546	SO:0001023	denotes	allele
T6143	3581-3588	SO:0000112	denotes	primers
T6144	3678-3680	SO:0000028	denotes	bp

craft-sa-dev

Id	Subject	Object	Predicate	Lexical cue
T5236	9-17	NN	denotes	Antibody
T5237	36-44	NNS	denotes	reagents
T5238	18-21	CC	denotes	and
T5239	22-26	NN	denotes	cell
T5240	27-35	NN	denotes	staining
T5241	44-298	sentence	denotes	Nestin (clone Rat 401, 2 μg/ml), Lamp 1 (clone 1D4B, 6 μg/ml), and Lamp 2 (clone Abl-93; 6 μg/ml) monoclonal antibodies were obtained from the Developmental Studies Hybridoma Bank, maintained by The University of Iowa, Department of Biological Sciences.
T5242	45-51	NN	denotes	Nestin
T5243	154-164	NNS	denotes	antibodies
T5244	52-53	-LRB-	denotes	(
T5245	70-72	NN	denotes	μg
T5246	53-58	NN	denotes	clone
T5247	59-62	NN	denotes	Rat
T5248	63-66	CD	denotes	401
T5249	66-68	,	denotes	,
T5250	68-69	CD	denotes	2
T5251	72-73	SYM	denotes	/
T5252	73-75	NN	denotes	ml
T5253	75-76	-RRB-	denotes	)
T5254	76-78	,	denotes	,
T5255	78-82	NN	denotes	Lamp
T5256	83-84	CD	denotes	1
T5257	85-86	-LRB-	denotes	(
T5258	100-102	NN	denotes	μg
T5259	86-91	NN	denotes	clone
T5260	92-96	NN	denotes	1D4B
T5261	96-98	,	denotes	,
T5262	98-99	CD	denotes	6
T5263	102-103	SYM	denotes	/
T5264	103-105	NN	denotes	ml
T5265	105-106	-RRB-	denotes	)
T5266	106-108	,	denotes	,
T5267	108-111	CC	denotes	and
T5268	112-116	NN	denotes	Lamp
T5269	117-118	CD	denotes	2
T5270	119-120	-LRB-	denotes	(
T5271	136-138	NN	denotes	μg
T5272	120-125	NN	denotes	clone
T5273	126-129	NN	denotes	Abl
T5274	129-130	HYPH	denotes	-
T5275	130-132	CD	denotes	93
T5276	132-133	:	denotes	;
T5277	134-135	CD	denotes	6
T5278	138-139	SYM	denotes	/
T5279	139-141	NN	denotes	ml
T5280	141-142	-RRB-	denotes	)
T5281	143-153	JJ	denotes	monoclonal
T5282	170-178	VBN	denotes	obtained
T5283	165-169	VBD	denotes	were
T5284	179-183	IN	denotes	from
T5285	184-187	DT	denotes	the
T5286	220-224	NNP	denotes	Bank
T5287	188-201	NNP	denotes	Developmental
T5288	202-209	NNPS	denotes	Studies
T5289	210-219	NNP	denotes	Hybridoma
T5290	224-226	,	denotes	,
T5291	226-236	VBN	denotes	maintained
T5292	237-239	IN	denotes	by
T5293	240-243	DT	denotes	The
T5294	244-254	NNP	denotes	University
T5295	264-274	NNP	denotes	Department
T5296	255-257	IN	denotes	of
T5297	258-262	NNP	denotes	Iowa
T5298	262-264	,	denotes	,
T5299	275-277	IN	denotes	of
T5300	278-288	NNP	denotes	Biological
T5301	289-297	NNPS	denotes	Sciences
T5302	297-298	.	denotes	.
T5303	298-345	sentence	denotes	Batp1 (1 μg/ml) was previously described [12].
T5304	299-304	NN	denotes	Batp1
T5305	330-339	VBN	denotes	described
T5306	305-306	-LRB-	denotes	(
T5307	308-310	NN	denotes	μg
T5308	306-307	CD	denotes	1
T5309	310-311	SYM	denotes	/
T5310	311-313	NN	denotes	ml
T5311	313-314	-RRB-	denotes	)
T5312	315-318	VBD	denotes	was
T5313	319-329	RB	denotes	previously
T5314	340-341	-LRB-	denotes	[
T5315	341-343	CD	denotes	12
T5316	343-344	-RRB-	denotes	]
T5317	344-345	.	denotes	.
T5318	345-454	sentence	denotes	Anti-subunit c antibody (0.7 μg/ml) was kindly provided by Dr. Kominami (Juntendo University, Tokyo, Japan).
T5319	346-358	JJ	denotes	Anti-subunit
T5320	359-360	NN	denotes	c
T5321	361-369	NN	denotes	antibody
T5322	393-401	VBN	denotes	provided
T5323	370-371	-LRB-	denotes	(
T5324	375-377	NN	denotes	μg
T5325	371-374	CD	denotes	0.7
T5326	377-378	SYM	denotes	/
T5327	378-380	NN	denotes	ml
T5328	380-381	-RRB-	denotes	)
T5329	382-385	VBD	denotes	was
T5330	386-392	RB	denotes	kindly
T5331	402-404	IN	denotes	by
T5332	405-408	NNP	denotes	Dr.
T5333	409-417	NNP	denotes	Kominami
T5334	418-419	-LRB-	denotes	(
T5335	428-438	NNP	denotes	University
T5336	419-427	NNP	denotes	Juntendo
T5337	438-440	,	denotes	,
T5338	440-445	NNP	denotes	Tokyo
T5339	445-447	,	denotes	,
T5340	447-452	NNP	denotes	Japan
T5341	452-453	-RRB-	denotes	)
T5342	453-454	.	denotes	.
T5343	454-1027	sentence	denotes	Additional antibodies were as follows: GFAP, 1:2000 (DAKO Corporation); calbindin, 1:5000 (Sigma); NeuN, 10 μg/ml (Chemicon); SV40 T antigen (Pab 101), 2 μg/ml (Santa Cruz Biotechnology); cathepsin D (C-20), 2 μg/ml (Santa Cruz Biotechnology); cytochrome c oxidase subunit IV (cox4), 1:1000 (BD Biosciences Clontech); PDI (H-160), 2 μg/ml (Santa Cruz Biotechnology); GM130, 1 μg/ml (BD Transduction Labs); α-tubulin, 1:15,000 (Sigma); grp75, 1:200 (Stressgen); early endosome antigen-1 (EEA1), 2 μg/ml (Santa Cruz Biotechnology); rab 7, 4 μg/ml (Santa Cruz Biotechnology).
T5344	455-465	JJ	denotes	Additional
T5345	466-476	NNS	denotes	antibodies
T5346	477-481	VBD	denotes	were
T5347	482-484	IN	denotes	as
T5348	485-492	VBZ	denotes	follows
T5349	492-494	:	denotes	:
T5350	494-498	NN	denotes	GFAP
T5351	498-500	,	denotes	,
T5352	500-501	CD	denotes	1
T5353	501-502	SYM	denotes	:
T5354	502-506	CD	denotes	2000
T5355	507-508	-LRB-	denotes	(
T5356	513-524	NN	denotes	Corporation
T5357	508-512	NN	denotes	DAKO
T5358	524-525	-RRB-	denotes	)
T5359	525-526	:	denotes	;
T5360	527-536	NN	denotes	calbindin
T5361	536-538	,	denotes	,
T5362	538-539	CD	denotes	1
T5363	539-540	SYM	denotes	:
T5364	540-544	CD	denotes	5000
T5365	545-546	-LRB-	denotes	(
T5366	546-551	NNP	denotes	Sigma
T5367	551-552	-RRB-	denotes	)
T5368	552-553	:	denotes	;
T5369	554-558	NN	denotes	NeuN
T5370	558-560	,	denotes	,
T5371	560-562	CD	denotes	10
T5372	563-565	NN	denotes	μg
T5373	565-566	SYM	denotes	/
T5374	566-568	NN	denotes	ml
T5375	569-570	-LRB-	denotes	(
T5376	570-578	NNP	denotes	Chemicon
T5377	578-579	-RRB-	denotes	)
T5378	579-580	:	denotes	;
T5379	581-585	NN	denotes	SV40
T5380	588-595	NN	denotes	antigen
T5381	586-587	NN	denotes	T
T5382	596-597	-LRB-	denotes	(
T5383	597-600	NN	denotes	Pab
T5384	601-604	CD	denotes	101
T5385	604-605	-RRB-	denotes	)
T5386	605-607	,	denotes	,
T5387	607-608	CD	denotes	2
T5388	609-611	NN	denotes	μg
T5389	611-612	SYM	denotes	/
T5390	612-614	NN	denotes	ml
T5391	615-616	-LRB-	denotes	(
T5392	627-640	NNP	denotes	Biotechnology
T5393	616-621	NNP	denotes	Santa
T5394	622-626	NNP	denotes	Cruz
T5395	640-641	-RRB-	denotes	)
T5396	641-642	:	denotes	;
T5397	643-652	NN	denotes	cathepsin
T5398	653-654	NN	denotes	D
T5399	655-656	-LRB-	denotes	(
T5400	656-657	NN	denotes	C
T5401	657-658	HYPH	denotes	-
T5402	658-660	CD	denotes	20
T5403	660-661	-RRB-	denotes	)
T5404	661-663	,	denotes	,
T5405	663-664	CD	denotes	2
T5406	665-667	NN	denotes	μg
T5407	667-668	SYM	denotes	/
T5408	668-670	NN	denotes	ml
T5409	671-672	-LRB-	denotes	(
T5410	683-696	NNP	denotes	Biotechnology
T5411	672-677	NNP	denotes	Santa
T5412	678-682	NNP	denotes	Cruz
T5413	696-697	-RRB-	denotes	)
T5414	697-698	:	denotes	;
T5415	699-709	NN	denotes	cytochrome
T5416	720-727	NN	denotes	subunit
T5417	710-711	NN	denotes	c
T5418	712-719	NN	denotes	oxidase
T5419	728-730	CD	denotes	IV
T5420	731-732	-LRB-	denotes	(
T5421	732-736	NN	denotes	cox4
T5422	736-737	-RRB-	denotes	)
T5423	737-739	,	denotes	,
T5424	739-740	CD	denotes	1
T5425	740-741	SYM	denotes	:
T5426	741-745	CD	denotes	1000
T5427	746-747	-LRB-	denotes	(
T5428	762-770	NNP	denotes	Clontech
T5429	747-749	NN	denotes	BD
T5430	750-761	NNP	denotes	Biosciences
T5431	770-771	-RRB-	denotes	)
T5432	771-772	:	denotes	;
T5433	773-776	NN	denotes	PDI
T5434	777-778	-LRB-	denotes	(
T5435	778-779	NN	denotes	H
T5436	779-780	HYPH	denotes	-
T5437	780-783	CD	denotes	160
T5438	783-784	-RRB-	denotes	)
T5439	784-786	,	denotes	,
T5440	786-787	CD	denotes	2
T5441	788-790	NN	denotes	μg
T5442	790-791	SYM	denotes	/
T5443	791-793	NN	denotes	ml
T5444	794-795	-LRB-	denotes	(
T5445	806-819	NNP	denotes	Biotechnology
T5446	795-800	NNP	denotes	Santa
T5447	801-805	NNP	denotes	Cruz
T5448	819-820	-RRB-	denotes	)
T5449	820-821	:	denotes	;
T5450	822-827	NN	denotes	GM130
T5451	827-829	,	denotes	,
T5452	829-830	CD	denotes	1
T5453	831-833	NN	denotes	μg
T5454	833-834	SYM	denotes	/
T5455	834-836	NN	denotes	ml
T5456	837-838	-LRB-	denotes	(
T5457	854-858	NNS	denotes	Labs
T5458	838-840	NN	denotes	BD
T5459	841-853	NN	denotes	Transduction
T5460	858-859	-RRB-	denotes	)
T5461	859-860	:	denotes	;
T5462	861-862	NN	denotes	α
T5463	863-870	NN	denotes	tubulin
T5464	862-863	HYPH	denotes	-
T5465	870-872	,	denotes	,
T5466	872-873	CD	denotes	1
T5467	873-874	SYM	denotes	:
T5468	874-880	CD	denotes	15,000
T5469	881-882	-LRB-	denotes	(
T5470	882-887	NNP	denotes	Sigma
T5471	887-888	-RRB-	denotes	)
T5472	888-889	:	denotes	;
T5473	890-895	NN	denotes	grp75
T5474	895-897	,	denotes	,
T5475	897-898	CD	denotes	1
T5476	898-899	SYM	denotes	:
T5477	899-902	CD	denotes	200
T5478	903-904	-LRB-	denotes	(
T5479	904-913	NNP	denotes	Stressgen
T5480	913-914	-RRB-	denotes	)
T5481	914-915	:	denotes	;
T5482	916-921	JJ	denotes	early
T5483	931-938	NN	denotes	antigen
T5484	922-930	NN	denotes	endosome
T5485	938-939	HYPH	denotes	-
T5486	939-940	CD	denotes	1
T5487	941-942	-LRB-	denotes	(
T5488	942-946	NN	denotes	EEA1
T5489	946-947	-RRB-	denotes	)
T5490	947-949	,	denotes	,
T5491	949-950	CD	denotes	2
T5492	951-953	NN	denotes	μg
T5493	953-954	SYM	denotes	/
T5494	954-956	NN	denotes	ml
T5495	957-958	-LRB-	denotes	(
T5496	969-982	NNP	denotes	Biotechnology
T5497	958-963	NNP	denotes	Santa
T5498	964-968	NNP	denotes	Cruz
T5499	982-983	-RRB-	denotes	)
T5500	983-984	:	denotes	;
T5501	985-988	NN	denotes	rab
T5502	989-990	CD	denotes	7
T5503	990-992	,	denotes	,
T5504	992-993	CD	denotes	4
T5505	994-996	NN	denotes	μg
T5506	996-997	SYM	denotes	/
T5507	997-999	NN	denotes	ml
T5508	1000-1001	-LRB-	denotes	(
T5509	1012-1025	NNP	denotes	Biotechnology
T5510	1001-1006	NNP	denotes	Santa
T5511	1007-1011	NNP	denotes	Cruz
T5512	1025-1026	-RRB-	denotes	)
T5513	1026-1027	.	denotes	.
T5514	1027-1200	sentence	denotes	All fluorescent secondary antibodies were obtained from Jackson ImmunoResearch Laboratories and HRP-conjugated secondary antibodies were obtained from Amersham Biosciences.
T5515	1028-1031	DT	denotes	All
T5516	1054-1064	NNS	denotes	antibodies
T5517	1032-1043	JJ	denotes	fluorescent
T5518	1044-1053	JJ	denotes	secondary
T5519	1070-1078	VBN	denotes	obtained
T5520	1065-1069	VBD	denotes	were
T5521	1079-1083	IN	denotes	from
T5522	1084-1091	NNP	denotes	Jackson
T5523	1107-1119	NNP	denotes	Laboratories
T5524	1092-1106	NNP	denotes	ImmunoResearch
T5525	1120-1123	CC	denotes	and
T5526	1124-1127	NN	denotes	HRP
T5527	1128-1138	VBN	denotes	conjugated
T5528	1127-1128	HYPH	denotes	-
T5529	1149-1159	NNS	denotes	antibodies
T5530	1139-1148	JJ	denotes	secondary
T5531	1165-1173	VBN	denotes	obtained
T5532	1160-1164	VBD	denotes	were
T5533	1174-1178	IN	denotes	from
T5534	1179-1187	NNP	denotes	Amersham
T5535	1188-1199	NNP	denotes	Biosciences
T5536	1199-1200	.	denotes	.
T5537	1200-1370	sentence	denotes	Additional cell markers were as follows: VVL-biotin, 1:2000 (Vector Laboratories), 10,000 MW dextran-FITC, 1 mg/ml and Lysotracker Red DND-99, 500 nM (Molecular Probes).
T5538	1201-1211	JJ	denotes	Additional
T5539	1217-1224	NNS	denotes	markers
T5540	1212-1216	NN	denotes	cell
T5541	1225-1229	VBD	denotes	were
T5542	1230-1232	IN	denotes	as
T5543	1233-1240	VBZ	denotes	follows
T5544	1240-1242	:	denotes	:
T5545	1242-1245	NN	denotes	VVL
T5546	1246-1252	NN	denotes	biotin
T5547	1245-1246	HYPH	denotes	-
T5548	1252-1254	,	denotes	,
T5549	1254-1255	CD	denotes	1
T5550	1255-1256	SYM	denotes	:
T5551	1256-1260	CD	denotes	2000
T5552	1261-1262	-LRB-	denotes	(
T5553	1269-1281	NNP	denotes	Laboratories
T5554	1262-1268	NNP	denotes	Vector
T5555	1281-1282	-RRB-	denotes	)
T5556	1282-1284	,	denotes	,
T5557	1284-1290	CD	denotes	10,000
T5558	1291-1293	NN	denotes	MW
T5559	1302-1306	NN	denotes	FITC
T5560	1294-1301	NN	denotes	dextran
T5561	1301-1302	HYPH	denotes	-
T5562	1306-1308	,	denotes	,
T5563	1308-1309	CD	denotes	1
T5564	1310-1312	NN	denotes	mg
T5565	1312-1313	SYM	denotes	/
T5566	1313-1315	NN	denotes	ml
T5567	1316-1319	CC	denotes	and
T5568	1320-1331	NNP	denotes	Lysotracker
T5569	1336-1339	NN	denotes	DND
T5570	1332-1335	NN	denotes	Red
T5571	1339-1340	HYPH	denotes	-
T5572	1340-1342	CD	denotes	99
T5573	1342-1344	,	denotes	,
T5574	1344-1347	CD	denotes	500
T5575	1348-1350	NN	denotes	nM
T5576	1351-1352	-LRB-	denotes	(
T5577	1362-1368	NNP	denotes	Probes
T5578	1352-1361	NNP	denotes	Molecular
T5579	1368-1369	-RRB-	denotes	)
T5580	1369-1370	.	denotes	.
T5720	1456-1461	NNS	denotes	lines
T5713	1372-1382	NN	denotes	Generation
T5714	1382-1384	,	denotes	,
T5715	1384-1395	NN	denotes	maintenance
T5716	1396-1399	CC	denotes	and
T5717	1400-1415	NN	denotes	differentiation
T5718	1416-1418	IN	denotes	of
T5719	1419-1425	NN	denotes	CbCln3
T5721	1426-1436	JJ	denotes	cerebellar
T5722	1437-1445	JJ	denotes	neuronal
T5723	1446-1455	NN	denotes	precursor
T5724	1461-1523	sentence	denotes	Cln3Δex7/8 knock-in mice have been previously described [12].
T5725	1462-1470	NN	denotes	Cln3Δex7
T5726	1482-1486	NNS	denotes	mice
T5727	1470-1471	HYPH	denotes	/
T5728	1471-1472	CD	denotes	8
T5729	1473-1478	VB	denotes	knock
T5730	1478-1479	HYPH	denotes	-
T5731	1479-1481	RP	denotes	in
T5732	1508-1517	VBN	denotes	described
T5733	1487-1491	VBP	denotes	have
T5734	1492-1496	VBN	denotes	been
T5735	1497-1507	RB	denotes	previously
T5736	1518-1519	-LRB-	denotes	[
T5737	1519-1521	CD	denotes	12
T5738	1521-1522	-RRB-	denotes	]
T5739	1522-1523	.	denotes	.
T5740	1523-1703	sentence	denotes	Littermate pups from heterozygote crosses were used for primary culture establishment, by previously described methods that yield cerebellar granule neuron-enriched cultures [26].
T5741	1524-1534	NN	denotes	Littermate
T5742	1535-1539	NNS	denotes	pups
T5743	1571-1575	VBN	denotes	used
T5744	1540-1544	IN	denotes	from
T5745	1545-1557	NN	denotes	heterozygote
T5746	1558-1565	NNS	denotes	crosses
T5747	1566-1570	VBD	denotes	were
T5748	1576-1579	IN	denotes	for
T5749	1580-1587	JJ	denotes	primary
T5750	1596-1609	NN	denotes	establishment
T5751	1588-1595	NN	denotes	culture
T5752	1609-1611	,	denotes	,
T5753	1611-1613	IN	denotes	by
T5754	1614-1624	RB	denotes	previously
T5755	1625-1634	VBN	denotes	described
T5756	1635-1642	NNS	denotes	methods
T5757	1643-1647	WDT	denotes	that
T5758	1648-1653	VBP	denotes	yield
T5759	1654-1664	JJ	denotes	cerebellar
T5760	1673-1679	NN	denotes	neuron
T5761	1665-1672	NN	denotes	granule
T5762	1680-1688	VBN	denotes	enriched
T5763	1679-1680	HYPH	denotes	-
T5764	1689-1697	NNS	denotes	cultures
T5765	1698-1699	-LRB-	denotes	[
T5766	1699-1701	CD	denotes	26
T5767	1701-1702	-RRB-	denotes	]
T5768	1702-1703	.	denotes	.
T5769	1703-1830	sentence	denotes	Postnatal day 4 (P4) cerebella were dissected in Hank's Balanced Salt Solution (HBSS, Sigma), supplemented with 35 mM glucose.
T5770	1704-1713	JJ	denotes	Postnatal
T5771	1714-1717	NN	denotes	day
T5772	1725-1734	NNS	denotes	cerebella
T5773	1718-1719	CD	denotes	4
T5774	1720-1721	-LRB-	denotes	(
T5775	1721-1723	NN	denotes	P4
T5776	1723-1724	-RRB-	denotes	)
T5777	1740-1749	VBN	denotes	dissected
T5778	1735-1739	VBD	denotes	were
T5779	1750-1752	IN	denotes	in
T5780	1753-1757	NNP	denotes	Hank
T5781	1774-1782	NNP	denotes	Solution
T5782	1757-1759	POS	denotes	's
T5783	1760-1768	NNP	denotes	Balanced
T5784	1769-1773	NNP	denotes	Salt
T5785	1783-1784	-LRB-	denotes	(
T5786	1784-1788	NN	denotes	HBSS
T5787	1788-1790	,	denotes	,
T5788	1790-1795	NNP	denotes	Sigma
T5789	1795-1796	-RRB-	denotes	)
T5790	1796-1798	,	denotes	,
T5791	1798-1810	VBN	denotes	supplemented
T5792	1811-1815	IN	denotes	with
T5793	1816-1818	CD	denotes	35
T5794	1819-1821	NN	denotes	mM
T5795	1822-1829	NN	denotes	glucose
T5796	1829-1830	.	denotes	.
T5797	1830-1914	sentence	denotes	Tail biopsies were also collected for genomic DNA isolation and genotypic analysis.
T5798	1831-1835	NN	denotes	Tail
T5799	1836-1844	NNS	denotes	biopsies
T5800	1855-1864	VBN	denotes	collected
T5801	1845-1849	VBD	denotes	were
T5802	1850-1854	RB	denotes	also
T5803	1865-1868	IN	denotes	for
T5804	1869-1876	JJ	denotes	genomic
T5805	1881-1890	NN	denotes	isolation
T5806	1877-1880	NN	denotes	DNA
T5807	1891-1894	CC	denotes	and
T5808	1895-1904	JJ	denotes	genotypic
T5809	1905-1913	NN	denotes	analysis
T5810	1913-1914	.	denotes	.
T5811	1914-2108	sentence	denotes	Trypsin/EDTA (10 mg/ml, Sigma) and DNase I (100 μg/ml, Sigma), suspended in HBSS, helped dissociate cerebella for primary culture plating onto 0.01% poly-ornithine (Sigma) coated 100 mm dishes.
T5812	1915-1922	NN	denotes	Trypsin
T5813	1923-1927	NN	denotes	EDTA
T5814	1922-1923	HYPH	denotes	/
T5815	1997-2003	VBD	denotes	helped
T5816	1928-1929	-LRB-	denotes	(
T5817	1939-1944	NNP	denotes	Sigma
T5818	1929-1931	CD	denotes	10
T5819	1932-1934	NN	denotes	mg
T5820	1934-1935	SYM	denotes	/
T5821	1935-1937	NN	denotes	ml
T5822	1937-1939	,	denotes	,
T5823	1944-1945	-RRB-	denotes	)
T5824	1946-1949	CC	denotes	and
T5825	1950-1955	NN	denotes	DNase
T5826	1956-1957	CD	denotes	I
T5827	1958-1959	-LRB-	denotes	(
T5828	1970-1975	NNP	denotes	Sigma
T5829	1959-1962	CD	denotes	100
T5830	1963-1965	NN	denotes	μg
T5831	1965-1966	SYM	denotes	/
T5832	1966-1968	NN	denotes	ml
T5833	1968-1970	,	denotes	,
T5834	1975-1976	-RRB-	denotes	)
T5835	1976-1978	,	denotes	,
T5836	1978-1987	VBN	denotes	suspended
T5837	1988-1990	IN	denotes	in
T5838	1991-1995	NN	denotes	HBSS
T5839	1995-1997	,	denotes	,
T5840	2004-2014	VB	denotes	dissociate
T5841	2015-2024	NNS	denotes	cerebella
T5842	2025-2028	IN	denotes	for
T5843	2029-2036	JJ	denotes	primary
T5844	2037-2044	NN	denotes	culture
T5845	2045-2052	NN	denotes	plating
T5846	2053-2057	IN	denotes	onto
T5847	2058-2062	CD	denotes	0.01
T5848	2062-2063	NN	denotes	%
T5849	2064-2078	NN	denotes	poly-ornithine
T5850	2087-2093	VBN	denotes	coated
T5851	2079-2080	-LRB-	denotes	(
T5852	2080-2085	NNP	denotes	Sigma
T5853	2085-2086	-RRB-	denotes	)
T5854	2101-2107	NNS	denotes	dishes
T5855	2094-2097	CD	denotes	100
T5856	2098-2100	NN	denotes	mm
T5857	2107-2108	.	denotes	.
T5858	2108-2357	sentence	denotes	Primary cultures from individual cerebella were cultured overnight at 37°C, 5% CO2, in granule neuron growth media (Dulbecco's Modified Eagle Medium [DMEM, Gibco BRL #11995-065], 10% fetal bovine serum [Sigma #F-2442], supplemented with 24 mM KCl).
T5859	2109-2116	JJ	denotes	Primary
T5860	2117-2125	NNS	denotes	cultures
T5861	2157-2165	VBN	denotes	cultured
T5862	2126-2130	IN	denotes	from
T5863	2131-2141	JJ	denotes	individual
T5864	2142-2151	NNS	denotes	cerebella
T5865	2152-2156	VBD	denotes	were
T5866	2166-2175	RB	denotes	overnight
T5867	2176-2178	IN	denotes	at
T5868	2179-2181	CD	denotes	37
T5869	2181-2183	NN	denotes	°C
T5870	2183-2185	,	denotes	,
T5871	2185-2186	CD	denotes	5
T5872	2186-2187	NN	denotes	%
T5873	2188-2191	NN	denotes	CO2
T5874	2191-2193	,	denotes	,
T5875	2193-2195	IN	denotes	in
T5876	2196-2203	NN	denotes	granule
T5877	2204-2210	NN	denotes	neuron
T5878	2218-2223	NNS	denotes	media
T5879	2211-2217	NN	denotes	growth
T5880	2224-2225	-LRB-	denotes	(
T5881	2225-2233	NNP	denotes	Dulbecco
T5882	2251-2257	NNP	denotes	Medium
T5883	2233-2235	POS	denotes	's
T5884	2236-2244	NNP	denotes	Modified
T5885	2245-2250	NNP	denotes	Eagle
T5886	2258-2259	-LRB-	denotes	[
T5887	2259-2263	NN	denotes	DMEM
T5888	2263-2265	,	denotes	,
T5889	2265-2270	NNP	denotes	Gibco
T5890	2275-2276	NN	denotes	#
T5891	2271-2274	NNP	denotes	BRL
T5892	2276-2281	CD	denotes	11995
T5893	2281-2282	HYPH	denotes	-
T5894	2282-2285	CD	denotes	065
T5895	2285-2286	-RRB-	denotes	]
T5896	2286-2288	,	denotes	,
T5897	2288-2290	CD	denotes	10
T5898	2290-2291	NN	denotes	%
T5899	2292-2297	JJ	denotes	fetal
T5900	2305-2310	NN	denotes	serum
T5901	2298-2304	JJ	denotes	bovine
T5902	2311-2312	-LRB-	denotes	[
T5903	2312-2317	NNP	denotes	Sigma
T5904	2319-2320	NN	denotes	F
T5905	2318-2319	NN	denotes	#
T5906	2320-2321	HYPH	denotes	-
T5907	2321-2325	CD	denotes	2442
T5908	2325-2326	-RRB-	denotes	]
T5909	2326-2328	,	denotes	,
T5910	2328-2340	VBN	denotes	supplemented
T5911	2341-2345	IN	denotes	with
T5912	2346-2348	CD	denotes	24
T5913	2349-2351	NN	denotes	mM
T5914	2352-2355	NN	denotes	KCl
T5915	2355-2356	-RRB-	denotes	)
T5916	2356-2357	.	denotes	.
T5917	2357-2570	sentence	denotes	Infection was performed the following day with defective retrovirus transducing the temperature-sensitive tsA58/U19 large T antigen and a selection neomycin-resistance cassette [27], as previously described [28].
T5918	2358-2367	NN	denotes	Infection
T5919	2372-2381	VBN	denotes	performed
T5920	2368-2371	VBD	denotes	was
T5921	2382-2385	DT	denotes	the
T5922	2396-2399	NN	denotes	day
T5923	2386-2395	JJ	denotes	following
T5924	2400-2404	IN	denotes	with
T5925	2405-2414	JJ	denotes	defective
T5926	2415-2425	NN	denotes	retrovirus
T5927	2426-2437	VBG	denotes	transducing
T5928	2438-2441	DT	denotes	the
T5929	2482-2489	NN	denotes	antigen
T5930	2442-2453	NN	denotes	temperature
T5931	2454-2463	JJ	denotes	sensitive
T5932	2453-2454	HYPH	denotes	-
T5933	2464-2469	NN	denotes	tsA58
T5934	2470-2473	NN	denotes	U19
T5935	2469-2470	HYPH	denotes	/
T5936	2474-2479	JJ	denotes	large
T5937	2480-2481	NN	denotes	T
T5938	2490-2493	CC	denotes	and
T5939	2494-2495	DT	denotes	a
T5940	2526-2534	NN	denotes	cassette
T5941	2496-2505	NN	denotes	selection
T5942	2506-2514	NN	denotes	neomycin
T5943	2515-2525	NN	denotes	resistance
T5944	2514-2515	HYPH	denotes	-
T5945	2535-2536	-LRB-	denotes	[
T5946	2536-2538	CD	denotes	27
T5947	2538-2539	-RRB-	denotes	]
T5948	2539-2541	,	denotes	,
T5949	2541-2543	IN	denotes	as
T5950	2555-2564	VBN	denotes	described
T5951	2544-2554	RB	denotes	previously
T5952	2565-2566	-LRB-	denotes	[
T5953	2566-2568	CD	denotes	28
T5954	2568-2569	-RRB-	denotes	]
T5955	2569-2570	.	denotes	.
T5956	2570-2742	sentence	denotes	Following infection, cultures were shifted to the tsA58/U19 permissive growth temperature of 33°C and selection was in the same growth media as above, with 200 μg/ml G418.
T5957	2571-2580	VBG	denotes	Following
T5958	2606-2613	VBN	denotes	shifted
T5959	2581-2590	NN	denotes	infection
T5960	2590-2592	,	denotes	,
T5961	2592-2600	NNS	denotes	cultures
T5962	2601-2605	VBD	denotes	were
T5963	2614-2616	IN	denotes	to
T5964	2617-2620	DT	denotes	the
T5965	2649-2660	NN	denotes	temperature
T5966	2621-2626	NN	denotes	tsA58
T5967	2627-2630	NN	denotes	U19
T5968	2626-2627	HYPH	denotes	/
T5969	2631-2641	JJ	denotes	permissive
T5970	2642-2648	NN	denotes	growth
T5971	2661-2663	IN	denotes	of
T5972	2664-2666	CD	denotes	33
T5973	2666-2668	NN	denotes	°C
T5974	2669-2672	CC	denotes	and
T5975	2673-2682	NN	denotes	selection
T5976	2683-2686	VBD	denotes	was
T5977	2687-2689	IN	denotes	in
T5978	2690-2693	DT	denotes	the
T5979	2706-2711	NNS	denotes	media
T5980	2694-2698	JJ	denotes	same
T5981	2699-2705	NN	denotes	growth
T5982	2712-2714	IN	denotes	as
T5983	2715-2720	RB	denotes	above
T5984	2720-2722	,	denotes	,
T5985	2722-2726	IN	denotes	with
T5986	2727-2730	CD	denotes	200
T5987	2731-2733	NN	denotes	μg
T5988	2737-2741	NN	denotes	G418
T5989	2733-2734	SYM	denotes	/
T5990	2734-2736	NN	denotes	ml
T5991	2741-2742	.	denotes	.
T5992	2742-2881	sentence	denotes	Conditionally immortalized colonies were isolated 3–9 weeks post-infection and expanded for frozen stocks and further sub-clone isolation.
T5993	2743-2756	RB	denotes	Conditionally
T5994	2757-2769	VBN	denotes	immortalized
T5995	2770-2778	NNS	denotes	colonies
T5996	2784-2792	VBN	denotes	isolated
T5997	2779-2783	VBD	denotes	were
T5998	2793-2794	CD	denotes	3
T5999	2795-2796	CD	denotes	9
T6000	2794-2795	SYM	denotes	–
T6001	2797-2802	NNS	denotes	weeks
T6002	2803-2817	RB	denotes	post-infection
T6003	2818-2821	CC	denotes	and
T6004	2822-2830	VBN	denotes	expanded
T6005	2831-2834	IN	denotes	for
T6006	2835-2841	JJ	denotes	frozen
T6007	2842-2848	NNS	denotes	stocks
T6008	2849-2852	CC	denotes	and
T6009	2853-2860	JJ	denotes	further
T6010	2871-2880	NN	denotes	isolation
T6011	2861-2870	NN	denotes	sub-clone
T6012	2880-2881	.	denotes	.
T6013	2881-3014	sentence	denotes	Multiple clonal lines were obtained for each genotype and all phenotypes were confirmed in at least 2 independent CbCln3 cell lines.
T6014	2882-2890	JJ	denotes	Multiple
T6015	2898-2903	NNS	denotes	lines
T6016	2891-2897	JJ	denotes	clonal
T6017	2909-2917	VBN	denotes	obtained
T6018	2904-2908	VBD	denotes	were
T6019	2918-2921	IN	denotes	for
T6020	2922-2926	DT	denotes	each
T6021	2927-2935	NN	denotes	genotype
T6022	2936-2939	CC	denotes	and
T6023	2940-2943	DT	denotes	all
T6024	2944-2954	NNS	denotes	phenotypes
T6025	2955-2959	VBD	denotes	were
T6026	2960-2969	VBN	denotes	confirmed
T6027	2970-2972	IN	denotes	in
T6028	2973-2975	RB	denotes	at
T6029	2982-2983	CD	denotes	2
T6030	2976-2981	RBS	denotes	least
T6031	3008-3013	NNS	denotes	lines
T6032	2984-2995	JJ	denotes	independent
T6033	2996-3002	NN	denotes	CbCln3
T6034	3003-3007	NN	denotes	cell
T6035	3013-3014	.	denotes	.
T6036	3014-3139	sentence	denotes	CbCln3 cell lines were maintained on 0.01% poly-ornithine coated dishes at 30–90% confluency, in 33°C and 5% CO2 atmosphere.
T6037	3015-3021	NN	denotes	CbCln3
T6038	3027-3032	NNS	denotes	lines
T6039	3022-3026	NN	denotes	cell
T6040	3038-3048	VBN	denotes	maintained
T6041	3033-3037	VBD	denotes	were
T6042	3049-3051	IN	denotes	on
T6043	3052-3056	CD	denotes	0.01
T6044	3056-3057	NN	denotes	%
T6045	3080-3086	NNS	denotes	dishes
T6046	3058-3062	NN	denotes	poly
T6047	3063-3072	NN	denotes	ornithine
T6048	3062-3063	HYPH	denotes	-
T6049	3073-3079	VBN	denotes	coated
T6050	3087-3089	IN	denotes	at
T6051	3090-3092	CD	denotes	30
T6052	3093-3095	CD	denotes	90
T6053	3092-3093	SYM	denotes	–
T6054	3095-3096	NN	denotes	%
T6055	3097-3107	NN	denotes	confluency
T6056	3107-3109	,	denotes	,
T6057	3109-3111	IN	denotes	in
T6058	3112-3114	CD	denotes	33
T6059	3114-3116	NN	denotes	°C
T6060	3117-3120	CC	denotes	and
T6061	3121-3122	CD	denotes	5
T6062	3128-3138	NN	denotes	atmosphere
T6063	3122-3123	SYM	denotes	%
T6064	3124-3127	NN	denotes	CO2
T6065	3138-3139	.	denotes	.
T6066	3139-3230	sentence	denotes	Passage number was recorded (up to ~20 passages), but had no apparent impact on phenotype.
T6067	3140-3147	NN	denotes	Passage
T6068	3148-3154	NN	denotes	number
T6069	3159-3167	VBN	denotes	recorded
T6070	3155-3158	VBD	denotes	was
T6071	3168-3169	-LRB-	denotes	(
T6072	3179-3187	NNS	denotes	passages
T6073	3169-3171	IN	denotes	up
T6074	3176-3178	CD	denotes	20
T6075	3172-3174	IN	denotes	to
T6076	3175-3176	SYM	denotes	~
T6077	3187-3188	-RRB-	denotes	)
T6078	3188-3190	,	denotes	,
T6079	3190-3193	CC	denotes	but
T6080	3194-3197	VBD	denotes	had
T6081	3198-3200	DT	denotes	no
T6082	3210-3216	NN	denotes	impact
T6083	3201-3209	JJ	denotes	apparent
T6084	3217-3219	IN	denotes	on
T6085	3220-3229	NN	denotes	phenotype
T6086	3229-3230	.	denotes	.
T6087	3230-3398	sentence	denotes	Neuronal differentiation was as previously described [29] with the following cocktail: 10 ng/ml α-FGF, 250 μM IBMX, 200 nM TPA, 50 μM forskolin, 5 μM dopamine (Sigma).
T6088	3231-3239	JJ	denotes	Neuronal
T6089	3240-3255	NN	denotes	differentiation
T6090	3256-3259	VBD	denotes	was
T6091	3260-3262	IN	denotes	as
T6092	3274-3283	VBN	denotes	described
T6093	3263-3273	RB	denotes	previously
T6094	3284-3285	-LRB-	denotes	[
T6095	3285-3287	CD	denotes	29
T6096	3287-3288	-RRB-	denotes	]
T6097	3289-3293	IN	denotes	with
T6098	3294-3297	DT	denotes	the
T6099	3308-3316	NN	denotes	cocktail
T6100	3298-3307	JJ	denotes	following
T6101	3316-3318	:	denotes	:
T6102	3318-3320	CD	denotes	10
T6103	3321-3323	NN	denotes	ng
T6104	3329-3332	NN	denotes	FGF
T6105	3323-3324	SYM	denotes	/
T6106	3324-3326	NN	denotes	ml
T6107	3327-3328	NN	denotes	α
T6108	3328-3329	HYPH	denotes	-
T6109	3332-3334	,	denotes	,
T6110	3334-3337	CD	denotes	250
T6111	3338-3340	NN	denotes	μM
T6112	3341-3345	NN	denotes	IBMX
T6113	3345-3347	,	denotes	,
T6114	3347-3350	CD	denotes	200
T6115	3351-3353	NN	denotes	nM
T6116	3354-3357	NN	denotes	TPA
T6117	3357-3359	,	denotes	,
T6118	3359-3361	CD	denotes	50
T6119	3362-3364	NN	denotes	μM
T6120	3365-3374	NN	denotes	forskolin
T6121	3374-3376	,	denotes	,
T6122	3376-3377	CD	denotes	5
T6123	3378-3380	NN	denotes	μM
T6124	3381-3389	NN	denotes	dopamine
T6125	3390-3391	-LRB-	denotes	(
T6126	3391-3396	NNP	denotes	Sigma
T6127	3396-3397	-RRB-	denotes	)
T6128	3397-3398	.	denotes	.
T6176	3453-3457	NN	denotes	tail
T6165	3400-3410	NN	denotes	Genotyping
T6166	3411-3414	CC	denotes	and
T6167	3415-3417	NN	denotes	RT
T6168	3418-3421	NN	denotes	PCR
T6169	3417-3418	HYPH	denotes	-
T6170	3421-3519	sentence	denotes	Genomic DNA was extracted from tail biopsies and cell pellets as described (Cotman et al., 2002).
T6171	3422-3429	JJ	denotes	Genomic
T6172	3430-3433	NN	denotes	DNA
T6173	3438-3447	VBN	denotes	extracted
T6174	3434-3437	VBD	denotes	was
T6175	3448-3452	IN	denotes	from
T6177	3458-3466	NNS	denotes	biopsies
T6178	3467-3470	CC	denotes	and
T6179	3471-3475	NN	denotes	cell
T6180	3476-3483	NNS	denotes	pellets
T6181	3484-3486	IN	denotes	as
T6182	3487-3496	VBN	denotes	described
T6183	3497-3498	-LRB-	denotes	(
T6184	3498-3504	NNP	denotes	Cotman
T6185	3505-3507	FW	denotes	et
T6186	3508-3511	FW	denotes	al.
T6187	3511-3513	,	denotes	,
T6188	3513-3517	CD	denotes	2002
T6189	3517-3518	-RRB-	denotes	)
T6190	3518-3519	.	denotes	.
T6191	3519-3808	sentence	denotes	Cln3Δex7/8 knock-in allele PCR genotyping was with wild-type primers, WtF (5'-CAGCATCTCCTCAGGGCTA-3') and WtR (5'-CCAACATAGAAAGTAGGGTGTGC-3') to yield a ~250 bp band and knock-in primers, 552F (5'-GAGCTTTGTTCTGGTTGCCTTC-3') and Ex9RA (5'-GCAGTCTCTGCCTCGTTTTCT-3') to yield a ~500 bp band.
T6192	3520-3528	NN	denotes	Cln3Δex7
T6193	3540-3546	NN	denotes	allele
T6194	3528-3529	HYPH	denotes	/
T6195	3529-3530	CD	denotes	8
T6196	3531-3536	VB	denotes	knock
T6197	3536-3537	HYPH	denotes	-
T6198	3537-3539	RP	denotes	in
T6199	3551-3561	NN	denotes	genotyping
T6200	3547-3550	NN	denotes	PCR
T6201	3562-3565	VBD	denotes	was
T6202	3566-3570	IN	denotes	with
T6203	3571-3575	JJ	denotes	wild
T6204	3576-3580	NN	denotes	type
T6205	3575-3576	HYPH	denotes	-
T6206	3581-3588	NNS	denotes	primers
T6207	3588-3590	,	denotes	,
T6208	3590-3593	NN	denotes	WtF
T6209	3594-3595	-LRB-	denotes	(
T6210	3595-3596	CD	denotes	5
T6211	3598-3617	NN	denotes	CAGCATCTCCTCAGGGCTA
T6212	3596-3597	SYM	denotes	'
T6213	3597-3598	HYPH	denotes	-
T6214	3617-3618	HYPH	denotes	-
T6215	3618-3619	CD	denotes	3
T6216	3619-3620	SYM	denotes	'
T6217	3620-3621	-RRB-	denotes	)
T6218	3622-3625	CC	denotes	and
T6219	3626-3629	NN	denotes	WtR
T6220	3630-3631	-LRB-	denotes	(
T6221	3631-3632	CD	denotes	5
T6222	3634-3657	NN	denotes	CCAACATAGAAAGTAGGGTGTGC
T6223	3632-3633	SYM	denotes	'
T6224	3633-3634	HYPH	denotes	-
T6225	3657-3658	HYPH	denotes	-
T6226	3658-3659	CD	denotes	3
T6227	3659-3660	SYM	denotes	'
T6228	3660-3661	-RRB-	denotes	)
T6229	3662-3664	TO	denotes	to
T6230	3665-3670	VB	denotes	yield
T6231	3671-3672	DT	denotes	a
T6232	3681-3685	NN	denotes	band
T6233	3673-3674	SYM	denotes	~
T6234	3674-3677	CD	denotes	250
T6235	3678-3680	NN	denotes	bp
T6236	3686-3689	CC	denotes	and
T6237	3690-3695	VB	denotes	knock
T6238	3699-3706	NNS	denotes	primers
T6239	3695-3696	HYPH	denotes	-
T6240	3696-3698	RP	denotes	in
T6241	3706-3708	,	denotes	,
T6242	3708-3712	NN	denotes	552F
T6243	3713-3714	-LRB-	denotes	(
T6244	3714-3715	CD	denotes	5
T6245	3717-3739	NN	denotes	GAGCTTTGTTCTGGTTGCCTTC
T6246	3715-3716	SYM	denotes	'
T6247	3716-3717	HYPH	denotes	-
T6248	3739-3740	HYPH	denotes	-
T6249	3740-3741	CD	denotes	3
T6250	3741-3742	SYM	denotes	'
T6251	3742-3743	-RRB-	denotes	)
T6252	3744-3747	CC	denotes	and
T6253	3748-3753	NN	denotes	Ex9RA
T6254	3754-3755	-LRB-	denotes	(
T6255	3755-3756	CD	denotes	5
T6256	3758-3779	NN	denotes	GCAGTCTCTGCCTCGTTTTCT
T6257	3756-3757	SYM	denotes	'
T6258	3757-3758	HYPH	denotes	-
T6259	3779-3780	HYPH	denotes	-
T6260	3780-3781	CD	denotes	3
T6261	3781-3782	SYM	denotes	'
T6262	3782-3783	-RRB-	denotes	)
T6263	3784-3786	TO	denotes	to
T6264	3787-3792	VB	denotes	yield
T6265	3793-3794	DT	denotes	a
T6266	3803-3807	NN	denotes	band
T6267	3795-3796	SYM	denotes	~
T6268	3796-3799	CD	denotes	500
T6269	3800-3802	NN	denotes	bp
T6270	3807-3808	.	denotes	.
T6271	3808-3927	sentence	denotes	PCR cycling conditions were 95°C for 30 seconds, 58°C for 30 seconds, and 72°C for 35 seconds, repeated for 34 cycles.
T6272	3809-3812	NN	denotes	PCR
T6273	3813-3820	NN	denotes	cycling
T6274	3821-3831	NNS	denotes	conditions
T6275	3832-3836	VBD	denotes	were
T6276	3837-3839	CD	denotes	95
T6277	3839-3841	NN	denotes	°C
T6278	3842-3845	IN	denotes	for
T6279	3846-3848	CD	denotes	30
T6280	3849-3856	NNS	denotes	seconds
T6281	3856-3858	,	denotes	,
T6282	3858-3860	CD	denotes	58
T6283	3860-3862	NN	denotes	°C
T6284	3863-3866	IN	denotes	for
T6285	3867-3869	CD	denotes	30
T6286	3870-3877	NNS	denotes	seconds
T6287	3877-3879	,	denotes	,
T6288	3879-3882	CC	denotes	and
T6289	3883-3885	CD	denotes	72
T6290	3885-3887	NN	denotes	°C
T6291	3888-3891	IN	denotes	for
T6292	3892-3894	CD	denotes	35
T6293	3895-3902	NNS	denotes	seconds
T6294	3902-3904	,	denotes	,
T6295	3904-3912	VBN	denotes	repeated
T6296	3913-3916	IN	denotes	for
T6297	3917-3919	CD	denotes	34
T6298	3920-3926	NNS	denotes	cycles
T6299	3926-3927	.	denotes	.
T6300	3927-4023	sentence	denotes	Total RNA isolation and Cln3 RT-PCR primers and procedures have been previously described [12].
T6301	3928-3933	JJ	denotes	Total
T6302	3938-3947	NN	denotes	isolation
T6303	3934-3937	NN	denotes	RNA
T6304	4008-4017	VBN	denotes	described
T6305	3948-3951	CC	denotes	and
T6306	3952-3956	NN	denotes	Cln3
T6307	3964-3971	NNS	denotes	primers
T6308	3957-3959	NN	denotes	RT
T6309	3960-3963	NN	denotes	PCR
T6310	3959-3960	HYPH	denotes	-
T6311	3972-3975	CC	denotes	and
T6312	3976-3986	NNS	denotes	procedures
T6313	3987-3991	VBP	denotes	have
T6314	3992-3996	VBN	denotes	been
T6315	3997-4007	RB	denotes	previously
T6316	4018-4019	-LRB-	denotes	[
T6317	4019-4021	CD	denotes	12
T6318	4021-4022	-RRB-	denotes	]
T6319	4022-4023	.	denotes	.
T6369	4025-4032	NN	denotes	Subunit
T6370	4033-4034	NN	denotes	c
T6371	4035-4047	NN	denotes	accumulation
T6372	4048-4053	NN	denotes	assay
T6373	4053-4264	sentence	denotes	Cells were seeded into 4-well chamber-slides (Falcon) at a density of 5 × 104 cells per well for microscopy studies, or into 100 mm dishes (Falcon) at a density of 5 × 105 cells per dish for protein extraction.
T6374	4054-4059	NNS	denotes	Cells
T6375	4065-4071	VBN	denotes	seeded
T6376	4060-4064	VBD	denotes	were
T6377	4072-4076	IN	denotes	into
T6378	4077-4078	CD	denotes	4
T6379	4079-4083	NN	denotes	well
T6380	4078-4079	HYPH	denotes	-
T6381	4092-4098	NNS	denotes	slides
T6382	4084-4091	NN	denotes	chamber
T6383	4091-4092	HYPH	denotes	-
T6384	4099-4100	-LRB-	denotes	(
T6385	4100-4106	NNP	denotes	Falcon
T6386	4106-4107	-RRB-	denotes	)
T6387	4108-4110	IN	denotes	at
T6388	4111-4112	DT	denotes	a
T6389	4113-4120	NN	denotes	density
T6390	4121-4123	IN	denotes	of
T6391	4124-4125	CD	denotes	5
T6392	4128-4131	CD	denotes	104
T6393	4126-4127	SYM	denotes	×
T6394	4132-4137	NNS	denotes	cells
T6395	4138-4141	IN	denotes	per
T6396	4142-4146	NN	denotes	well
T6397	4147-4150	IN	denotes	for
T6398	4151-4161	NN	denotes	microscopy
T6399	4162-4169	NNS	denotes	studies
T6400	4169-4171	,	denotes	,
T6401	4171-4173	CC	denotes	or
T6402	4174-4178	IN	denotes	into
T6403	4179-4182	CD	denotes	100
T6404	4183-4185	NN	denotes	mm
T6405	4186-4192	NNS	denotes	dishes
T6406	4193-4194	-LRB-	denotes	(
T6407	4194-4200	NNP	denotes	Falcon
T6408	4200-4201	-RRB-	denotes	)
T6409	4202-4204	IN	denotes	at
T6410	4205-4206	DT	denotes	a
T6411	4207-4214	NN	denotes	density
T6412	4215-4217	IN	denotes	of
T6413	4218-4219	CD	denotes	5
T6414	4222-4225	CD	denotes	105
T6415	4220-4221	SYM	denotes	×
T6416	4226-4231	NNS	denotes	cells
T6417	4232-4235	IN	denotes	per
T6418	4236-4240	NN	denotes	dish
T6419	4241-4244	IN	denotes	for
T6420	4245-4252	NN	denotes	protein
T6421	4253-4263	NN	denotes	extraction
T6422	4263-4264	.	denotes	.
T6423	4264-4382	sentence	denotes	Cells were typically >95% confluent one day post-plating, and the following day was considered 1-day post-confluency.
T6424	4265-4270	NNS	denotes	Cells
T6425	4271-4275	VBD	denotes	were
T6426	4276-4285	RB	denotes	typically
T6427	4286-4287	SYM	denotes	>
T6428	4287-4289	CD	denotes	95
T6429	4289-4290	NN	denotes	%
T6430	4291-4300	JJ	denotes	confluent
T6431	4301-4304	CD	denotes	one
T6432	4305-4308	NN	denotes	day
T6433	4309-4321	RB	denotes	post-plating
T6434	4321-4323	,	denotes	,
T6435	4323-4326	CC	denotes	and
T6436	4327-4330	DT	denotes	the
T6437	4341-4344	NN	denotes	day
T6438	4331-4340	JJ	denotes	following
T6439	4349-4359	VBN	denotes	considered
T6440	4345-4348	VBD	denotes	was
T6441	4360-4361	CD	denotes	1
T6442	4362-4365	NN	denotes	day
T6443	4361-4362	HYPH	denotes	-
T6444	4366-4381	RB	denotes	post-confluency
T6445	4381-4382	.	denotes	.
T6446	4382-4632	sentence	denotes	At the indicated times, cells were either fixed with 4% formaldehyde in phosphate buffered saline (PBS), pH 7.4, for 20 minutes and processed for autofluorescence/subunit c immunostaining, or cell pellets were collected for total protein extraction.
T6447	4383-4385	IN	denotes	At
T6448	4425-4430	VBN	denotes	fixed
T6449	4386-4389	DT	denotes	the
T6450	4400-4405	NNS	denotes	times
T6451	4390-4399	VBN	denotes	indicated
T6452	4405-4407	,	denotes	,
T6453	4407-4412	NNS	denotes	cells
T6454	4413-4417	VBD	denotes	were
T6455	4418-4424	RB	denotes	either
T6456	4431-4435	IN	denotes	with
T6457	4436-4437	CD	denotes	4
T6458	4437-4438	NN	denotes	%
T6459	4439-4451	NN	denotes	formaldehyde
T6460	4452-4454	IN	denotes	in
T6461	4455-4464	NN	denotes	phosphate
T6462	4474-4480	NN	denotes	saline
T6463	4465-4473	VBN	denotes	buffered
T6464	4481-4482	-LRB-	denotes	(
T6465	4482-4485	NN	denotes	PBS
T6466	4485-4486	-RRB-	denotes	)
T6467	4486-4488	,	denotes	,
T6468	4488-4490	NN	denotes	pH
T6469	4491-4494	CD	denotes	7.4
T6470	4494-4496	,	denotes	,
T6471	4496-4499	IN	denotes	for
T6472	4500-4502	CD	denotes	20
T6473	4503-4510	NNS	denotes	minutes
T6474	4511-4514	CC	denotes	and
T6475	4515-4524	VBN	denotes	processed
T6476	4525-4528	IN	denotes	for
T6477	4529-4545	NN	denotes	autofluorescence
T6478	4545-4546	HYPH	denotes	/
T6479	4546-4553	NN	denotes	subunit
T6480	4554-4555	NN	denotes	c
T6481	4556-4570	NN	denotes	immunostaining
T6482	4570-4572	,	denotes	,
T6483	4572-4574	CC	denotes	or
T6484	4575-4579	NN	denotes	cell
T6485	4580-4587	NNS	denotes	pellets
T6486	4593-4602	VBN	denotes	collected
T6487	4588-4592	VBD	denotes	were
T6488	4603-4606	IN	denotes	for
T6489	4607-4612	JJ	denotes	total
T6490	4621-4631	NN	denotes	extraction
T6491	4613-4620	NN	denotes	protein
T6492	4631-4632	.	denotes	.
T6493	4632-4833	sentence	denotes	Alternatively, cells were fixed with 2.5% glutaraldehyde/2% paraformaldehyde in 0.1 M cacodylate buffer, pH 7.4 for 1 hour and subsequently post-fixed and processed for TEM analysis as described [12].
T6494	4633-4646	RB	denotes	Alternatively
T6495	4659-4664	VBN	denotes	fixed
T6496	4646-4648	,	denotes	,
T6497	4648-4653	NNS	denotes	cells
T6498	4654-4658	VBD	denotes	were
T6499	4665-4669	IN	denotes	with
T6500	4670-4673	CD	denotes	2.5
T6501	4673-4674	NN	denotes	%
T6502	4675-4689	NN	denotes	glutaraldehyde
T6503	4689-4690	HYPH	denotes	/
T6504	4690-4691	CD	denotes	2
T6505	4691-4692	NN	denotes	%
T6506	4693-4709	NN	denotes	paraformaldehyde
T6507	4710-4712	IN	denotes	in
T6508	4713-4716	CD	denotes	0.1
T6509	4717-4718	NN	denotes	M
T6510	4730-4736	NN	denotes	buffer
T6511	4719-4729	NN	denotes	cacodylate
T6512	4736-4738	,	denotes	,
T6513	4738-4740	NN	denotes	pH
T6514	4741-4744	CD	denotes	7.4
T6515	4745-4748	IN	denotes	for
T6516	4749-4750	CD	denotes	1
T6517	4751-4755	NN	denotes	hour
T6518	4756-4759	CC	denotes	and
T6519	4760-4772	RB	denotes	subsequently
T6520	4773-4783	VBN	denotes	post-fixed
T6521	4784-4787	CC	denotes	and
T6522	4788-4797	VBN	denotes	processed
T6523	4798-4801	IN	denotes	for
T6524	4802-4805	NN	denotes	TEM
T6525	4806-4814	NN	denotes	analysis
T6526	4815-4817	IN	denotes	as
T6527	4818-4827	VBN	denotes	described
T6528	4828-4829	-LRB-	denotes	[
T6529	4829-4831	CD	denotes	12
T6530	4831-4832	-RRB-	denotes	]
T6531	4832-4833	.	denotes	.
T6532	4833-4928	sentence	denotes	In confocal microscopy studies, autofluorescent signal was observed over multiple wavelengths.
T6533	4834-4836	IN	denotes	In
T6534	4893-4901	VBN	denotes	observed
T6535	4837-4845	JJ	denotes	confocal
T6536	4857-4864	NNS	denotes	studies
T6537	4846-4856	NN	denotes	microscopy
T6538	4864-4866	,	denotes	,
T6539	4866-4881	JJ	denotes	autofluorescent
T6540	4882-4888	NN	denotes	signal
T6541	4889-4892	VBD	denotes	was
T6542	4902-4906	IN	denotes	over
T6543	4907-4915	JJ	denotes	multiple
T6544	4916-4927	NNS	denotes	wavelengths
T6545	4927-4928	.	denotes	.
T6546	4928-5045	sentence	denotes	For co-staining, settings were reduced such that autofluorescent signal did not contribute to antibody label signal.
T6547	4929-4932	IN	denotes	For
T6548	4960-4967	VBN	denotes	reduced
T6549	4933-4944	NN	denotes	co-staining
T6550	4944-4946	,	denotes	,
T6551	4946-4954	NNS	denotes	settings
T6552	4955-4959	VBD	denotes	were
T6553	4968-4972	JJ	denotes	such
T6554	4973-4977	IN	denotes	that
T6555	5009-5019	VB	denotes	contribute
T6556	4978-4993	JJ	denotes	autofluorescent
T6557	4994-5000	NN	denotes	signal
T6558	5001-5004	VBD	denotes	did
T6559	5005-5008	RB	denotes	not
T6560	5020-5022	IN	denotes	to
T6561	5023-5031	NN	denotes	antibody
T6562	5038-5044	NN	denotes	signal
T6563	5032-5037	NN	denotes	label
T6564	5044-5045	.	denotes	.
T6658	5047-5061	NN	denotes	Immunostaining
T6659	5077-5085	NN	denotes	analysis
T6660	5062-5065	CC	denotes	and
T6661	5066-5076	NN	denotes	Immunoblot
T6662	5085-5246	sentence	denotes	For immunostaining, cells were seeded at a density of 3–5 × 104 cells per well in 4-well chamber-slides and grown overnight at 33°C, unless indicated otherwise.
T6663	5086-5089	IN	denotes	For
T6664	5117-5123	VBN	denotes	seeded
T6665	5090-5104	NN	denotes	immunostaining
T6666	5104-5106	,	denotes	,
T6667	5106-5111	NNS	denotes	cells
T6668	5112-5116	VBD	denotes	were
T6669	5124-5126	IN	denotes	at
T6670	5127-5128	DT	denotes	a
T6671	5129-5136	NN	denotes	density
T6672	5137-5139	IN	denotes	of
T6673	5140-5141	CD	denotes	3
T6674	5142-5143	CD	denotes	5
T6675	5141-5142	SYM	denotes	–
T6676	5146-5149	CD	denotes	104
T6677	5144-5145	SYM	denotes	×
T6678	5150-5155	NNS	denotes	cells
T6679	5156-5159	IN	denotes	per
T6680	5160-5164	NN	denotes	well
T6681	5165-5167	IN	denotes	in
T6682	5168-5169	CD	denotes	4
T6683	5170-5174	NN	denotes	well
T6684	5169-5170	HYPH	denotes	-
T6685	5183-5189	NNS	denotes	slides
T6686	5175-5182	NN	denotes	chamber
T6687	5182-5183	HYPH	denotes	-
T6688	5190-5193	CC	denotes	and
T6689	5194-5199	VBN	denotes	grown
T6690	5200-5209	RB	denotes	overnight
T6691	5210-5212	IN	denotes	at
T6692	5213-5215	CD	denotes	33
T6693	5215-5217	NN	denotes	°C
T6694	5217-5219	,	denotes	,
T6695	5219-5225	IN	denotes	unless
T6696	5226-5235	VBN	denotes	indicated
T6697	5236-5245	RB	denotes	otherwise
T6698	5245-5246	.	denotes	.
T6699	5246-5430	sentence	denotes	Fixation was with ice-cold 4% formaldehyde in PBS, pH 7.4, for 20 minutes, or with ice-cold methanol/acetone (1:1) for 10 minutes at -20°C followed by air-drying (antibody-dependent).
T6700	5247-5255	NN	denotes	Fixation
T6701	5256-5259	VBD	denotes	was
T6702	5260-5264	IN	denotes	with
T6703	5265-5268	NN	denotes	ice
T6704	5269-5273	JJ	denotes	cold
T6705	5268-5269	HYPH	denotes	-
T6706	5277-5289	NN	denotes	formaldehyde
T6707	5274-5275	CD	denotes	4
T6708	5275-5276	NN	denotes	%
T6709	5290-5292	IN	denotes	in
T6710	5293-5296	NN	denotes	PBS
T6711	5296-5298	,	denotes	,
T6712	5298-5300	NN	denotes	pH
T6713	5301-5304	CD	denotes	7.4
T6714	5304-5306	,	denotes	,
T6715	5306-5309	IN	denotes	for
T6716	5310-5312	CD	denotes	20
T6717	5313-5320	NNS	denotes	minutes
T6718	5320-5322	,	denotes	,
T6719	5322-5324	CC	denotes	or
T6720	5325-5329	IN	denotes	with
T6721	5330-5333	NN	denotes	ice
T6722	5334-5338	JJ	denotes	cold
T6723	5333-5334	HYPH	denotes	-
T6724	5348-5355	NN	denotes	acetone
T6725	5339-5347	NN	denotes	methanol
T6726	5347-5348	HYPH	denotes	/
T6727	5356-5357	-LRB-	denotes	(
T6728	5357-5358	CD	denotes	1
T6729	5358-5359	SYM	denotes	:
T6730	5359-5360	CD	denotes	1
T6731	5360-5361	-RRB-	denotes	)
T6732	5362-5365	IN	denotes	for
T6733	5366-5368	CD	denotes	10
T6734	5369-5376	NNS	denotes	minutes
T6735	5377-5379	IN	denotes	at
T6736	5380-5381	SYM	denotes	-
T6737	5381-5383	CD	denotes	20
T6738	5383-5385	NN	denotes	°C
T6739	5386-5394	VBN	denotes	followed
T6740	5395-5397	IN	denotes	by
T6741	5398-5401	NN	denotes	air
T6742	5402-5408	NN	denotes	drying
T6743	5401-5402	HYPH	denotes	-
T6744	5409-5410	-LRB-	denotes	(
T6745	5419-5428	JJ	denotes	dependent
T6746	5410-5418	NN	denotes	antibody
T6747	5418-5419	HYPH	denotes	-
T6748	5428-5429	-RRB-	denotes	)
T6749	5429-5430	.	denotes	.
T6750	5430-5849	sentence	denotes	Cells were washed with PBS at least 2 times, 5 minutes per wash, between each of the following steps of the staining procedure: 0.1 M glycine in PBS for 5 minutes, 0.05% or 0.1% (antibody-dependent) Triton X-100 (Fisher Scientific) in PBS for 5 minutes, 2% bovine serum albumin (BSA) in PBS for 30 minutes, primary antibody diluted in 2% BSA/PBS for 90 minutes, secondary antibody diluted in 2% BSA/PBS for 60 minutes.
T6751	5431-5436	NNS	denotes	Cells
T6752	5442-5448	VBN	denotes	washed
T6753	5437-5441	VBD	denotes	were
T6754	5449-5453	IN	denotes	with
T6755	5454-5457	NN	denotes	PBS
T6756	5458-5460	RB	denotes	at
T6757	5467-5468	CD	denotes	2
T6758	5461-5466	RBS	denotes	least
T6759	5469-5474	NNS	denotes	times
T6760	5474-5476	,	denotes	,
T6761	5476-5477	CD	denotes	5
T6762	5478-5485	NNS	denotes	minutes
T6763	5486-5489	IN	denotes	per
T6764	5490-5494	NN	denotes	wash
T6765	5494-5496	,	denotes	,
T6766	5496-5503	IN	denotes	between
T6767	5504-5508	DT	denotes	each
T6768	5509-5511	IN	denotes	of
T6769	5512-5515	DT	denotes	the
T6770	5526-5531	NNS	denotes	steps
T6771	5516-5525	JJ	denotes	following
T6772	5532-5534	IN	denotes	of
T6773	5535-5538	DT	denotes	the
T6774	5548-5557	NN	denotes	procedure
T6775	5539-5547	NN	denotes	staining
T6776	5557-5559	:	denotes	:
T6777	5559-5562	CD	denotes	0.1
T6778	5563-5564	NN	denotes	M
T6779	5565-5572	NN	denotes	glycine
T6780	5573-5575	IN	denotes	in
T6781	5576-5579	NN	denotes	PBS
T6782	5580-5583	IN	denotes	for
T6783	5584-5585	CD	denotes	5
T6784	5586-5593	NNS	denotes	minutes
T6785	5593-5595	,	denotes	,
T6786	5595-5599	CD	denotes	0.05
T6787	5599-5600	NN	denotes	%
T6788	5637-5638	NN	denotes	X
T6789	5601-5603	CC	denotes	or
T6790	5604-5607	CD	denotes	0.1
T6791	5607-5608	NN	denotes	%
T6792	5609-5610	-LRB-	denotes	(
T6793	5610-5618	NN	denotes	antibody
T6794	5619-5628	JJ	denotes	dependent
T6795	5618-5619	HYPH	denotes	-
T6796	5628-5629	-RRB-	denotes	)
T6797	5630-5636	NN	denotes	Triton
T6798	5638-5639	HYPH	denotes	-
T6799	5639-5642	CD	denotes	100
T6800	5643-5644	-LRB-	denotes	(
T6801	5651-5661	NNP	denotes	Scientific
T6802	5644-5650	NNP	denotes	Fisher
T6803	5661-5662	-RRB-	denotes	)
T6804	5663-5665	IN	denotes	in
T6805	5666-5669	NN	denotes	PBS
T6806	5670-5673	IN	denotes	for
T6807	5674-5675	CD	denotes	5
T6808	5676-5683	NNS	denotes	minutes
T6809	5683-5685	,	denotes	,
T6810	5685-5686	CD	denotes	2
T6811	5686-5687	NN	denotes	%
T6812	5688-5694	JJ	denotes	bovine
T6813	5701-5708	NN	denotes	albumin
T6814	5695-5700	NN	denotes	serum
T6815	5709-5710	-LRB-	denotes	(
T6816	5710-5713	NN	denotes	BSA
T6817	5713-5714	-RRB-	denotes	)
T6818	5715-5717	IN	denotes	in
T6819	5718-5721	NN	denotes	PBS
T6820	5722-5725	IN	denotes	for
T6821	5726-5728	CD	denotes	30
T6822	5729-5736	NNS	denotes	minutes
T6823	5736-5738	,	denotes	,
T6824	5738-5745	JJ	denotes	primary
T6825	5746-5754	NN	denotes	antibody
T6826	5755-5762	VBN	denotes	diluted
T6827	5763-5765	IN	denotes	in
T6828	5766-5767	CD	denotes	2
T6829	5767-5768	NN	denotes	%
T6830	5773-5776	NN	denotes	PBS
T6831	5769-5772	NN	denotes	BSA
T6832	5772-5773	HYPH	denotes	/
T6833	5777-5780	IN	denotes	for
T6834	5781-5783	CD	denotes	90
T6835	5784-5791	NNS	denotes	minutes
T6836	5791-5793	,	denotes	,
T6837	5793-5802	JJ	denotes	secondary
T6838	5803-5811	NN	denotes	antibody
T6839	5812-5819	VBN	denotes	diluted
T6840	5820-5822	IN	denotes	in
T6841	5823-5824	CD	denotes	2
T6842	5824-5825	NN	denotes	%
T6843	5830-5833	NN	denotes	PBS
T6844	5826-5829	NN	denotes	BSA
T6845	5829-5830	HYPH	denotes	/
T6846	5834-5837	IN	denotes	for
T6847	5838-5840	CD	denotes	60
T6848	5841-5848	NNS	denotes	minutes
T6849	5848-5849	.	denotes	.
T6850	5849-5903	sentence	denotes	All incubations were carried out at room temperature.
T6851	5850-5853	DT	denotes	All
T6852	5854-5865	NNS	denotes	incubations
T6853	5871-5878	VBN	denotes	carried
T6854	5866-5870	VBD	denotes	were
T6855	5879-5882	RP	denotes	out
T6856	5883-5885	IN	denotes	at
T6857	5886-5890	NN	denotes	room
T6858	5891-5902	NN	denotes	temperature
T6859	5902-5903	.	denotes	.
T6860	5903-6154	sentence	denotes	Following staining procedures, slides were coverslipped with Vectashield mounting medium (Vector Laboratories) and analyzed on a BioRad Radiance 2100 confocal microscope (Biorad), with identical exposure settings for wild-type and mutant like images.
T6861	5904-5913	VBG	denotes	Following
T6862	5947-5959	VBN	denotes	coverslipped
T6863	5914-5922	NN	denotes	staining
T6864	5923-5933	NNS	denotes	procedures
T6865	5933-5935	,	denotes	,
T6866	5935-5941	NNS	denotes	slides
T6867	5942-5946	VBD	denotes	were
T6868	5960-5964	IN	denotes	with
T6869	5965-5976	NNP	denotes	Vectashield
T6870	5986-5992	NN	denotes	medium
T6871	5977-5985	NN	denotes	mounting
T6872	5993-5994	-LRB-	denotes	(
T6873	6001-6013	NNP	denotes	Laboratories
T6874	5994-6000	NNP	denotes	Vector
T6875	6013-6014	-RRB-	denotes	)
T6876	6015-6018	CC	denotes	and
T6877	6019-6027	VBN	denotes	analyzed
T6878	6028-6030	IN	denotes	on
T6879	6031-6032	DT	denotes	a
T6880	6063-6073	NN	denotes	microscope
T6881	6033-6039	NNP	denotes	BioRad
T6882	6040-6048	NNP	denotes	Radiance
T6883	6049-6053	CD	denotes	2100
T6884	6054-6062	JJ	denotes	confocal
T6885	6074-6075	-LRB-	denotes	(
T6886	6075-6081	NNP	denotes	Biorad
T6887	6081-6082	-RRB-	denotes	)
T6888	6082-6084	,	denotes	,
T6889	6084-6088	IN	denotes	with
T6890	6089-6098	JJ	denotes	identical
T6891	6108-6116	NNS	denotes	settings
T6892	6099-6107	NN	denotes	exposure
T6893	6117-6120	IN	denotes	for
T6894	6121-6125	JJ	denotes	wild
T6895	6126-6130	NN	denotes	type
T6896	6125-6126	HYPH	denotes	-
T6897	6147-6153	NNS	denotes	images
T6898	6131-6134	CC	denotes	and
T6899	6135-6141	JJ	denotes	mutant
T6900	6142-6146	JJ	denotes	like
T6901	6153-6154	.	denotes	.
T6902	6154-6289	sentence	denotes	All comparisons of wild-type and mutant staining were performed in Adobe Photoshop with identical brightness and contrast adjustments.
T6903	6155-6158	DT	denotes	All
T6904	6159-6170	NNS	denotes	comparisons
T6905	6209-6218	VBN	denotes	performed
T6906	6171-6173	IN	denotes	of
T6907	6174-6178	JJ	denotes	wild
T6908	6179-6183	NN	denotes	type
T6909	6178-6179	HYPH	denotes	-
T6910	6195-6203	NN	denotes	staining
T6911	6184-6187	CC	denotes	and
T6912	6188-6194	NN	denotes	mutant
T6913	6204-6208	VBD	denotes	were
T6914	6219-6221	IN	denotes	in
T6915	6222-6227	NNP	denotes	Adobe
T6916	6228-6237	NNP	denotes	Photoshop
T6917	6238-6242	IN	denotes	with
T6918	6243-6252	JJ	denotes	identical
T6919	6277-6288	NNS	denotes	adjustments
T6920	6253-6263	NN	denotes	brightness
T6921	6264-6267	CC	denotes	and
T6922	6268-6276	NN	denotes	contrast
T6923	6288-6289	.	denotes	.
T6924	6289-6552	sentence	denotes	Total proteins were isolated from cell pellets by extraction with ice-cold 20 mM Tris, pH 7.4, 1% Triton X-100 (membrane-research grade, Roche), plus protease inhibitors (Complete mini tablet, 0.7 μg/ml pepstatin A, 2 μg/ml aprotinin, 5 μg/ml leupeptin [Roche]).
T6925	6290-6295	JJ	denotes	Total
T6926	6296-6304	NN	denotes	proteins
T6927	6310-6318	VBN	denotes	isolated
T6928	6305-6309	VBD	denotes	were
T6929	6319-6323	IN	denotes	from
T6930	6324-6328	NN	denotes	cell
T6931	6329-6336	NNS	denotes	pellets
T6932	6337-6339	IN	denotes	by
T6933	6340-6350	NN	denotes	extraction
T6934	6351-6355	IN	denotes	with
T6935	6356-6359	NN	denotes	ice
T6936	6371-6375	NN	denotes	Tris
T6937	6359-6360	HYPH	denotes	-
T6938	6360-6364	JJ	denotes	cold
T6939	6365-6367	CD	denotes	20
T6940	6368-6370	NN	denotes	mM
T6941	6375-6377	,	denotes	,
T6942	6377-6379	NN	denotes	pH
T6943	6380-6383	CD	denotes	7.4
T6944	6383-6385	,	denotes	,
T6945	6385-6386	CD	denotes	1
T6946	6386-6387	NN	denotes	%
T6947	6395-6396	NN	denotes	X
T6948	6388-6394	NN	denotes	Triton
T6949	6396-6397	HYPH	denotes	-
T6950	6397-6400	CD	denotes	100
T6951	6401-6402	-LRB-	denotes	(
T6952	6427-6432	NNP	denotes	Roche
T6953	6402-6410	NN	denotes	membrane
T6954	6411-6419	NN	denotes	research
T6955	6410-6411	HYPH	denotes	-
T6956	6420-6425	NN	denotes	grade
T6957	6425-6427	,	denotes	,
T6958	6432-6433	-RRB-	denotes	)
T6959	6433-6435	,	denotes	,
T6960	6435-6439	CC	denotes	plus
T6961	6440-6448	NN	denotes	protease
T6962	6449-6459	NNS	denotes	inhibitors
T6963	6460-6461	-LRB-	denotes	(
T6964	6461-6469	JJ	denotes	Complete
T6965	6475-6481	NN	denotes	tablet
T6966	6470-6474	NN	denotes	mini
T6967	6481-6483	,	denotes	,
T6968	6483-6486	CD	denotes	0.7
T6969	6487-6489	NN	denotes	μg
T6970	6503-6504	NN	denotes	A
T6971	6489-6490	SYM	denotes	/
T6972	6490-6492	NN	denotes	ml
T6973	6493-6502	NN	denotes	pepstatin
T6974	6504-6506	,	denotes	,
T6975	6506-6507	CD	denotes	2
T6976	6508-6510	NN	denotes	μg
T6977	6514-6523	NN	denotes	aprotinin
T6978	6510-6511	SYM	denotes	/
T6979	6511-6513	NN	denotes	ml
T6980	6523-6525	,	denotes	,
T6981	6525-6526	CD	denotes	5
T6982	6527-6529	NN	denotes	μg
T6983	6533-6542	NN	denotes	leupeptin
T6984	6529-6530	SYM	denotes	/
T6985	6530-6532	NN	denotes	ml
T6986	6543-6544	-LRB-	denotes	[
T6987	6544-6549	NNP	denotes	Roche
T6988	6549-6550	-RRB-	denotes	]
T6989	6550-6551	-RRB-	denotes	)
T6990	6551-6552	.	denotes	.
T6991	6552-6697	sentence	denotes	Following homogenization through a 25-gauge needle (~10 passes), extracts were centrifuged at 1000 × g for 10 minutes, at 4°C, to remove debris.
T6992	6553-6562	VBG	denotes	Following
T6993	6632-6643	VBN	denotes	centrifuged
T6994	6563-6577	NN	denotes	homogenization
T6995	6578-6585	IN	denotes	through
T6996	6586-6587	DT	denotes	a
T6997	6597-6603	NN	denotes	needle
T6998	6588-6590	CD	denotes	25
T6999	6591-6596	NN	denotes	gauge
T7000	6590-6591	HYPH	denotes	-
T7001	6604-6605	-LRB-	denotes	(
T7002	6609-6615	NNS	denotes	passes
T7003	6605-6606	SYM	denotes	~
T7004	6606-6608	CD	denotes	10
T7005	6615-6616	-RRB-	denotes	)
T7006	6616-6618	,	denotes	,
T7007	6618-6626	NNS	denotes	extracts
T7008	6627-6631	VBD	denotes	were
T7009	6644-6646	IN	denotes	at
T7010	6647-6651	CD	denotes	1000
T7011	6654-6655	NN	denotes	g
T7012	6652-6653	SYM	denotes	×
T7013	6656-6659	IN	denotes	for
T7014	6660-6662	CD	denotes	10
T7015	6663-6670	NNS	denotes	minutes
T7016	6670-6672	,	denotes	,
T7017	6672-6674	IN	denotes	at
T7018	6675-6676	CD	denotes	4
T7019	6676-6678	NN	denotes	°C
T7020	6678-6680	,	denotes	,
T7021	6680-6682	TO	denotes	to
T7022	6683-6689	VB	denotes	remove
T7023	6690-6696	NN	denotes	debris
T7024	6696-6697	.	denotes	.
T7025	6697-6846	sentence	denotes	Typically, 20–40 μg of protein (determined by Bio-rad Dc Protein Assay) was separated by SDS-PAGE, for subsequent immunoblotting, as described [12].
T7026	6698-6707	RB	denotes	Typically
T7027	6774-6783	VBN	denotes	separated
T7028	6707-6709	,	denotes	,
T7029	6709-6711	CD	denotes	20
T7030	6712-6714	CD	denotes	40
T7031	6711-6712	SYM	denotes	–
T7032	6715-6717	NN	denotes	μg
T7033	6718-6720	IN	denotes	of
T7034	6721-6728	NN	denotes	protein
T7035	6729-6730	-LRB-	denotes	(
T7036	6730-6740	VBN	denotes	determined
T7037	6741-6743	IN	denotes	by
T7038	6744-6747	NNP	denotes	Bio
T7039	6748-6751	NNP	denotes	rad
T7040	6747-6748	HYPH	denotes	-
T7041	6763-6768	NN	denotes	Assay
T7042	6752-6754	NN	denotes	Dc
T7043	6755-6762	NN	denotes	Protein
T7044	6768-6769	-RRB-	denotes	)
T7045	6770-6773	VBD	denotes	was
T7046	6784-6786	IN	denotes	by
T7047	6787-6790	NN	denotes	SDS
T7048	6791-6795	NN	denotes	PAGE
T7049	6790-6791	HYPH	denotes	-
T7050	6795-6797	,	denotes	,
T7051	6797-6800	IN	denotes	for
T7052	6801-6811	JJ	denotes	subsequent
T7053	6812-6826	NN	denotes	immunoblotting
T7054	6826-6828	,	denotes	,
T7055	6828-6830	IN	denotes	as
T7056	6831-6840	VBN	denotes	described
T7057	6841-6842	-LRB-	denotes	[
T7058	6842-6844	CD	denotes	12
T7059	6844-6845	-RRB-	denotes	]
T7060	6845-6846	.	denotes	.
T7061	6846-6931	sentence	denotes	16.5% tris-tricine SDS-PAGE gels were used for subunit c immunoblotting experiments.
T7062	6847-6851	CD	denotes	16.5
T7063	6851-6852	NN	denotes	%
T7064	6875-6879	NNS	denotes	gels
T7065	6853-6857	NN	denotes	tris
T7066	6858-6865	NN	denotes	tricine
T7067	6857-6858	HYPH	denotes	-
T7068	6866-6869	NN	denotes	SDS
T7069	6870-6874	NN	denotes	PAGE
T7070	6869-6870	HYPH	denotes	-
T7071	6885-6889	VBN	denotes	used
T7072	6880-6884	VBD	denotes	were
T7073	6890-6893	IN	denotes	for
T7074	6894-6901	NN	denotes	subunit
T7075	6902-6903	NN	denotes	c
T7076	6919-6930	NNS	denotes	experiments
T7077	6904-6918	NN	denotes	immunoblotting
T7078	6930-6931	.	denotes	.
T7116	6933-6942	NN	denotes	Cathepsin
T7117	6943-6944	NN	denotes	D
T7118	6954-6959	NN	denotes	assay
T7119	6945-6953	NN	denotes	activity
T7120	6959-7220	sentence	denotes	100 mm tissue culture dishes, which were approximately 80–90% confluent, were washed briefly with ice-cold PBS, and total protein extracts were isolated by scraping cells into 10 mM Tris, pH 7.4, 0.1% Triton X-100 followed by incubation on ice, for 20 minutes.
T7121	6960-6963	CD	denotes	100
T7122	6964-6966	NN	denotes	mm
T7123	6982-6988	NNS	denotes	dishes
T7124	6967-6973	NN	denotes	tissue
T7125	6974-6981	NN	denotes	culture
T7126	7038-7044	VBN	denotes	washed
T7127	6988-6990	,	denotes	,
T7128	6990-6995	WDT	denotes	which
T7129	6996-7000	VBD	denotes	were
T7130	7001-7014	RB	denotes	approximately
T7131	7018-7020	CD	denotes	90
T7132	7015-7017	CD	denotes	80
T7133	7017-7018	SYM	denotes	–
T7134	7020-7021	NN	denotes	%
T7135	7022-7031	JJ	denotes	confluent
T7136	7031-7033	,	denotes	,
T7137	7033-7037	VBD	denotes	were
T7138	7045-7052	RB	denotes	briefly
T7139	7053-7057	IN	denotes	with
T7140	7058-7061	NN	denotes	ice
T7141	7062-7066	JJ	denotes	cold
T7142	7061-7062	HYPH	denotes	-
T7143	7067-7070	NN	denotes	PBS
T7144	7070-7072	,	denotes	,
T7145	7072-7075	CC	denotes	and
T7146	7076-7081	JJ	denotes	total
T7147	7090-7098	NNS	denotes	extracts
T7148	7082-7089	NN	denotes	protein
T7149	7104-7112	VBN	denotes	isolated
T7150	7099-7103	VBD	denotes	were
T7151	7113-7115	IN	denotes	by
T7152	7116-7124	VBG	denotes	scraping
T7153	7125-7130	NNS	denotes	cells
T7154	7131-7135	IN	denotes	into
T7155	7136-7138	CD	denotes	10
T7156	7139-7141	NN	denotes	mM
T7157	7142-7146	NN	denotes	Tris
T7158	7146-7148	,	denotes	,
T7159	7148-7150	NN	denotes	pH
T7160	7151-7154	CD	denotes	7.4
T7161	7154-7156	,	denotes	,
T7162	7156-7159	CD	denotes	0.1
T7163	7159-7160	NN	denotes	%
T7164	7168-7169	NN	denotes	X
T7165	7161-7167	NN	denotes	Triton
T7166	7169-7170	HYPH	denotes	-
T7167	7170-7173	CD	denotes	100
T7168	7174-7182	VBN	denotes	followed
T7169	7183-7185	IN	denotes	by
T7170	7186-7196	NN	denotes	incubation
T7171	7197-7199	IN	denotes	on
T7172	7200-7203	NN	denotes	ice
T7173	7203-7205	,	denotes	,
T7174	7205-7208	IN	denotes	for
T7175	7209-7211	CD	denotes	20
T7176	7212-7219	NNS	denotes	minutes
T7177	7219-7220	.	denotes	.
T7178	7220-7379	sentence	denotes	The insoluble material was centrifuged at 14,000 g, the supernatant was isolated, and protein concentration was determined using the Bio-rad Dc Protein Assay.
T7179	7221-7224	DT	denotes	The
T7180	7235-7243	NN	denotes	material
T7181	7225-7234	JJ	denotes	insoluble
T7182	7248-7259	VBN	denotes	centrifuged
T7183	7244-7247	VBD	denotes	was
T7184	7260-7262	IN	denotes	at
T7185	7263-7269	CD	denotes	14,000
T7186	7270-7271	NN	denotes	g
T7187	7271-7273	,	denotes	,
T7188	7273-7276	DT	denotes	the
T7189	7277-7288	NN	denotes	supernatant
T7190	7293-7301	VBN	denotes	isolated
T7191	7289-7292	VBD	denotes	was
T7192	7301-7303	,	denotes	,
T7193	7303-7306	CC	denotes	and
T7194	7307-7314	NN	denotes	protein
T7195	7315-7328	NN	denotes	concentration
T7196	7333-7343	VBN	denotes	determined
T7197	7329-7332	VBD	denotes	was
T7198	7344-7349	VBG	denotes	using
T7199	7350-7353	DT	denotes	the
T7200	7373-7378	NN	denotes	Assay
T7201	7354-7357	NNP	denotes	Bio
T7202	7358-7361	NNP	denotes	rad
T7203	7357-7358	HYPH	denotes	-
T7204	7362-7364	NN	denotes	Dc
T7205	7365-7372	NN	denotes	Protein
T7206	7378-7379	.	denotes	.
T7207	7379-7599	sentence	denotes	50–70 μg of total protein extract were used to measure cathepsin D activity using the Fluorogenic Innozyme™ Cathepsin D Immunocapture Activity Assay Kit (EMD Biosciences) according to the manufacturer's recommendations.
T7208	7380-7382	CD	denotes	50
T7209	7383-7385	CD	denotes	70
T7210	7382-7383	SYM	denotes	–
T7211	7386-7388	NN	denotes	μg
T7212	7419-7423	VBN	denotes	used
T7213	7389-7391	IN	denotes	of
T7214	7392-7397	JJ	denotes	total
T7215	7406-7413	NN	denotes	extract
T7216	7398-7405	NN	denotes	protein
T7217	7414-7418	VBD	denotes	were
T7218	7424-7426	TO	denotes	to
T7219	7427-7434	VB	denotes	measure
T7220	7435-7444	NN	denotes	cathepsin
T7221	7445-7446	NN	denotes	D
T7222	7447-7455	NN	denotes	activity
T7223	7456-7461	VBG	denotes	using
T7224	7462-7465	DT	denotes	the
T7225	7529-7532	NN	denotes	Kit
T7226	7466-7477	JJ	denotes	Fluorogenic
T7227	7498-7499	NN	denotes	D
T7228	7478-7486	NNP	denotes	Innozyme
T7229	7486-7487	SYM	denotes	™
T7230	7488-7497	NN	denotes	Cathepsin
T7231	7500-7513	NN	denotes	Immunocapture
T7232	7514-7522	NN	denotes	Activity
T7233	7523-7528	NN	denotes	Assay
T7234	7533-7534	-LRB-	denotes	(
T7235	7538-7549	NNP	denotes	Biosciences
T7236	7534-7537	NNP	denotes	EMD
T7237	7549-7550	-RRB-	denotes	)
T7238	7551-7560	VBG	denotes	according
T7239	7561-7563	IN	denotes	to
T7240	7564-7567	DT	denotes	the
T7241	7568-7580	NN	denotes	manufacturer
T7242	7583-7598	NNS	denotes	recommendations
T7243	7580-7582	POS	denotes	's
T7244	7598-7599	.	denotes	.
T7245	7599-7873	sentence	denotes	Relative fluorescence was measured using an Analyst AD plate reader (Molecular Devices) with the following filters and settings: excitation filter, 360-35; emission filter, 400-20, Flash lamp with 100 readings/well, 100 ms between readings, and 100,000 μs integration time.
T7246	7600-7608	JJ	denotes	Relative
T7247	7609-7621	NN	denotes	fluorescence
T7248	7626-7634	VBN	denotes	measured
T7249	7622-7625	VBD	denotes	was
T7250	7635-7640	VBG	denotes	using
T7251	7641-7643	DT	denotes	an
T7252	7661-7667	NN	denotes	reader
T7253	7644-7651	NN	denotes	Analyst
T7254	7652-7654	NN	denotes	AD
T7255	7655-7660	NN	denotes	plate
T7256	7668-7669	-LRB-	denotes	(
T7257	7679-7686	NNPS	denotes	Devices
T7258	7669-7678	JJ	denotes	Molecular
T7259	7686-7687	-RRB-	denotes	)
T7260	7688-7692	IN	denotes	with
T7261	7693-7696	DT	denotes	the
T7262	7707-7714	NNS	denotes	filters
T7263	7697-7706	JJ	denotes	following
T7264	7715-7718	CC	denotes	and
T7265	7719-7727	NNS	denotes	settings
T7266	7727-7729	:	denotes	:
T7267	7729-7739	NN	denotes	excitation
T7268	7740-7746	NN	denotes	filter
T7269	7752-7754	CD	denotes	35
T7270	7746-7748	,	denotes	,
T7271	7748-7751	CD	denotes	360
T7272	7751-7752	HYPH	denotes	-
T7273	7754-7755	:	denotes	;
T7274	7756-7764	NN	denotes	emission
T7275	7765-7771	NN	denotes	filter
T7276	7777-7779	CD	denotes	20
T7277	7771-7773	,	denotes	,
T7278	7773-7776	CD	denotes	400
T7279	7776-7777	HYPH	denotes	-
T7280	7779-7781	,	denotes	,
T7281	7781-7786	NN	denotes	Flash
T7282	7787-7791	NN	denotes	lamp
T7283	7792-7796	IN	denotes	with
T7284	7797-7800	CD	denotes	100
T7285	7801-7809	NNS	denotes	readings
T7286	7809-7810	SYM	denotes	/
T7287	7810-7814	NN	denotes	well
T7288	7814-7816	,	denotes	,
T7289	7816-7819	CD	denotes	100
T7290	7820-7822	NN	denotes	ms
T7291	7823-7830	IN	denotes	between
T7292	7831-7839	NNS	denotes	readings
T7293	7839-7841	,	denotes	,
T7294	7841-7844	CC	denotes	and
T7295	7845-7852	CD	denotes	100,000
T7296	7853-7855	NN	denotes	μs
T7297	7868-7872	NN	denotes	time
T7298	7856-7867	NN	denotes	integration
T7299	7872-7873	.	denotes	.
T7337	7875-7884	JJ	denotes	Lysosomal
T7338	7885-7893	NN	denotes	staining
T7339	7894-7897	CC	denotes	and
T7340	7898-7907	JJ	denotes	endocytic
T7341	7908-7914	NN	denotes	uptake
T7342	7914-8027	sentence	denotes	Cells were seeded at a density of 3–5 × 104 cells per well in 4-well chamber-slides and grown overnight at 33°C.
T7343	7915-7920	NNS	denotes	Cells
T7344	7926-7932	VBN	denotes	seeded
T7345	7921-7925	VBD	denotes	were
T7346	7933-7935	IN	denotes	at
T7347	7936-7937	DT	denotes	a
T7348	7938-7945	NN	denotes	density
T7349	7946-7948	IN	denotes	of
T7350	7949-7950	CD	denotes	3
T7351	7951-7952	CD	denotes	5
T7352	7950-7951	SYM	denotes	–
T7353	7955-7958	CD	denotes	104
T7354	7953-7954	SYM	denotes	×
T7355	7959-7964	NNS	denotes	cells
T7356	7965-7968	IN	denotes	per
T7357	7969-7973	NN	denotes	well
T7358	7974-7976	IN	denotes	in
T7359	7977-7978	CD	denotes	4
T7360	7979-7983	NN	denotes	well
T7361	7978-7979	HYPH	denotes	-
T7362	7992-7998	NNS	denotes	slides
T7363	7984-7991	NN	denotes	chamber
T7364	7991-7992	HYPH	denotes	-
T7365	7999-8002	CC	denotes	and
T7366	8003-8008	VBN	denotes	grown
T7367	8009-8018	RB	denotes	overnight
T7368	8019-8021	IN	denotes	at
T7369	8022-8024	CD	denotes	33
T7370	8024-8026	NN	denotes	°C
T7371	8026-8027	.	denotes	.
T7372	8027-8221	sentence	denotes	Growth media was exchanged for fresh, pre-warmed growth media containing 500 nM Lysotracker or 1 mg/ml dextran-FITC, and cells were incubated at 33°C for 45 minutes or 15 minutes, respectively.
T7373	8028-8034	NN	denotes	Growth
T7374	8035-8040	NNS	denotes	media
T7375	8045-8054	VBN	denotes	exchanged
T7376	8041-8044	VBD	denotes	was
T7377	8055-8058	IN	denotes	for
T7378	8059-8064	JJ	denotes	fresh
T7379	8084-8089	NNS	denotes	media
T7380	8064-8066	,	denotes	,
T7381	8066-8076	VBN	denotes	pre-warmed
T7382	8077-8083	NN	denotes	growth
T7383	8090-8100	VBG	denotes	containing
T7384	8101-8104	CD	denotes	500
T7385	8105-8107	NN	denotes	nM
T7386	8108-8119	NNP	denotes	Lysotracker
T7387	8120-8122	CC	denotes	or
T7388	8123-8124	CD	denotes	1
T7389	8125-8127	NN	denotes	mg
T7390	8139-8143	NN	denotes	FITC
T7391	8127-8128	SYM	denotes	/
T7392	8128-8130	NN	denotes	ml
T7393	8131-8138	NN	denotes	dextran
T7394	8138-8139	HYPH	denotes	-
T7395	8143-8145	,	denotes	,
T7396	8145-8148	CC	denotes	and
T7397	8149-8154	NNS	denotes	cells
T7398	8160-8169	VBN	denotes	incubated
T7399	8155-8159	VBD	denotes	were
T7400	8170-8172	IN	denotes	at
T7401	8173-8175	CD	denotes	33
T7402	8175-8177	NN	denotes	°C
T7403	8178-8181	IN	denotes	for
T7404	8182-8184	CD	denotes	45
T7405	8185-8192	NNS	denotes	minutes
T7406	8193-8195	CC	denotes	or
T7407	8196-8198	CD	denotes	15
T7408	8199-8206	NNS	denotes	minutes
T7409	8206-8208	,	denotes	,
T7410	8208-8220	RB	denotes	respectively
T7411	8220-8221	.	denotes	.
T7412	8221-8394	sentence	denotes	Following labeling, cells were immediately placed on ice and washed for 10 minutes in ice-cold dye-free media, and fixed with 4% formaldehyde in PBS, for 20 minutes on ice.
T7413	8222-8231	VBG	denotes	Following
T7414	8265-8271	VBN	denotes	placed
T7415	8232-8240	NN	denotes	labeling
T7416	8240-8242	,	denotes	,
T7417	8242-8247	NNS	denotes	cells
T7418	8248-8252	VBD	denotes	were
T7419	8253-8264	RB	denotes	immediately
T7420	8272-8274	IN	denotes	on
T7421	8275-8278	NN	denotes	ice
T7422	8279-8282	CC	denotes	and
T7423	8283-8289	VBN	denotes	washed
T7424	8290-8293	IN	denotes	for
T7425	8294-8296	CD	denotes	10
T7426	8297-8304	NNS	denotes	minutes
T7427	8305-8307	IN	denotes	in
T7428	8308-8311	NN	denotes	ice
T7429	8312-8316	JJ	denotes	cold
T7430	8311-8312	HYPH	denotes	-
T7431	8326-8331	NNS	denotes	media
T7432	8317-8320	NN	denotes	dye
T7433	8321-8325	JJ	denotes	free
T7434	8320-8321	HYPH	denotes	-
T7435	8331-8333	,	denotes	,
T7436	8333-8336	CC	denotes	and
T7437	8337-8342	VBN	denotes	fixed
T7438	8343-8347	IN	denotes	with
T7439	8348-8349	CD	denotes	4
T7440	8349-8350	NN	denotes	%
T7441	8351-8363	NN	denotes	formaldehyde
T7442	8364-8366	IN	denotes	in
T7443	8367-8370	NN	denotes	PBS
T7444	8370-8372	,	denotes	,
T7445	8372-8375	IN	denotes	for
T7446	8376-8378	CD	denotes	20
T7447	8379-8386	NNS	denotes	minutes
T7448	8387-8389	IN	denotes	on
T7449	8390-8393	NN	denotes	ice
T7450	8393-8394	.	denotes	.
T7451	8394-8531	sentence	denotes	Chambers were removed and slides were coverslipped with Vectashield mounting media for confocal microscopy analysis, as described above.
T7452	8395-8403	NNS	denotes	Chambers
T7453	8409-8416	VBN	denotes	removed
T7454	8404-8408	VBD	denotes	were
T7455	8417-8420	CC	denotes	and
T7456	8421-8427	NNS	denotes	slides
T7457	8433-8445	VBN	denotes	coverslipped
T7458	8428-8432	VBD	denotes	were
T7459	8446-8450	IN	denotes	with
T7460	8451-8462	NNP	denotes	Vectashield
T7461	8472-8477	NNS	denotes	media
T7462	8463-8471	NN	denotes	mounting
T7463	8478-8481	IN	denotes	for
T7464	8482-8490	JJ	denotes	confocal
T7465	8491-8501	NN	denotes	microscopy
T7466	8502-8510	NN	denotes	analysis
T7467	8510-8512	,	denotes	,
T7468	8512-8514	IN	denotes	as
T7469	8515-8524	VBN	denotes	described
T7470	8525-8530	RB	denotes	above
T7471	8530-8531	.	denotes	.
T7484	8533-8545	JJ	denotes	Morphometric
T7485	8546-8554	NN	denotes	analysis
T7486	8555-8557	IN	denotes	of
T7487	8558-8570	NNS	denotes	mitochondria
T7488	8570-8663	sentence	denotes	TEM photomicrographs (10,000 × – 40,000 × magnification) were taken from random grid fields.
T7489	8571-8574	NN	denotes	TEM
T7490	8575-8591	NNS	denotes	photomicrographs
T7491	8633-8638	VBN	denotes	taken
T7492	8592-8593	-LRB-	denotes	(
T7493	8613-8626	NN	denotes	magnification
T7494	8593-8599	CD	denotes	10,000
T7495	8604-8610	CD	denotes	40,000
T7496	8600-8601	SYM	denotes	×
T7497	8602-8603	SYM	denotes	–
T7498	8611-8612	SYM	denotes	×
T7499	8626-8627	-RRB-	denotes	)
T7500	8628-8632	VBD	denotes	were
T7501	8639-8643	IN	denotes	from
T7502	8644-8650	JJ	denotes	random
T7503	8656-8662	NNS	denotes	fields
T7504	8651-8655	NN	denotes	grid
T7505	8662-8663	.	denotes	.
T7506	8663-8900	sentence	denotes	For length measurements, the longest side of each mitochondria was measured in centimeters, and along the length of the mitochondria, width measurements were taken every 2.5–4 mm (dependent on the magnification of the micrograph image).
T7507	8664-8667	IN	denotes	For
T7508	8731-8739	VBN	denotes	measured
T7509	8668-8674	NN	denotes	length
T7510	8675-8687	NNS	denotes	measurements
T7511	8687-8689	,	denotes	,
T7512	8689-8692	DT	denotes	the
T7513	8701-8705	NN	denotes	side
T7514	8693-8700	JJS	denotes	longest
T7515	8706-8708	IN	denotes	of
T7516	8709-8713	DT	denotes	each
T7517	8714-8726	NNS	denotes	mitochondria
T7518	8727-8730	VBD	denotes	was
T7519	8740-8742	IN	denotes	in
T7520	8743-8754	NNS	denotes	centimeters
T7521	8754-8756	,	denotes	,
T7522	8756-8759	CC	denotes	and
T7523	8760-8765	IN	denotes	along
T7524	8822-8827	VBN	denotes	taken
T7525	8766-8769	DT	denotes	the
T7526	8770-8776	NN	denotes	length
T7527	8777-8779	IN	denotes	of
T7528	8780-8783	DT	denotes	the
T7529	8784-8796	NNS	denotes	mitochondria
T7530	8796-8798	,	denotes	,
T7531	8798-8803	NN	denotes	width
T7532	8804-8816	NNS	denotes	measurements
T7533	8817-8821	VBD	denotes	were
T7534	8828-8833	DT	denotes	every
T7535	8840-8842	NN	denotes	mm
T7536	8834-8837	CD	denotes	2.5
T7537	8838-8839	CD	denotes	4
T7538	8837-8838	SYM	denotes	–
T7539	8843-8844	-LRB-	denotes	(
T7540	8844-8853	JJ	denotes	dependent
T7541	8854-8856	IN	denotes	on
T7542	8857-8860	DT	denotes	the
T7543	8861-8874	NN	denotes	magnification
T7544	8875-8877	IN	denotes	of
T7545	8878-8881	DT	denotes	the
T7546	8893-8898	NN	denotes	image
T7547	8882-8892	NN	denotes	micrograph
T7548	8898-8899	-RRB-	denotes	)
T7549	8899-8900	.	denotes	.
T7550	8900-9034	sentence	denotes	Following measurement, all numbers were normalized to reflect one magnification and data was analyzed using Microsoft Excel software.
T7551	8901-8910	VBG	denotes	Following
T7552	8941-8951	VBN	denotes	normalized
T7553	8911-8922	NN	denotes	measurement
T7554	8922-8924	,	denotes	,
T7555	8924-8927	DT	denotes	all
T7556	8928-8935	NNS	denotes	numbers
T7557	8936-8940	VBD	denotes	were
T7558	8952-8954	TO	denotes	to
T7559	8955-8962	VB	denotes	reflect
T7560	8963-8966	CD	denotes	one
T7561	8967-8980	NN	denotes	magnification
T7562	8981-8984	CC	denotes	and
T7563	8985-8989	NNS	denotes	data
T7564	8994-9002	VBN	denotes	analyzed
T7565	8990-8993	VBD	denotes	was
T7566	9003-9008	VBG	denotes	using
T7567	9009-9018	NNP	denotes	Microsoft
T7568	9019-9024	NNP	denotes	Excel
T7569	9025-9033	NN	denotes	software
T7570	9033-9034	.	denotes	.
T7571	9034-9186	sentence	denotes	To ascertain unmagnified mitochondrial size, final measurement data, in centimeters, was converted to nanometers according to scale bar representation.
T7572	9035-9037	TO	denotes	To
T7573	9038-9047	VB	denotes	ascertain
T7574	9124-9133	VBN	denotes	converted
T7575	9048-9059	JJ	denotes	unmagnified
T7576	9074-9078	NN	denotes	size
T7577	9060-9073	JJ	denotes	mitochondrial
T7578	9078-9080	,	denotes	,
T7579	9080-9085	JJ	denotes	final
T7580	9098-9102	NNS	denotes	data
T7581	9086-9097	NN	denotes	measurement
T7582	9102-9104	,	denotes	,
T7583	9104-9106	IN	denotes	in
T7584	9107-9118	NNS	denotes	centimeters
T7585	9118-9120	,	denotes	,
T7586	9120-9123	VBD	denotes	was
T7587	9134-9136	IN	denotes	to
T7588	9137-9147	NNS	denotes	nanometers
T7589	9148-9157	VBG	denotes	according
T7590	9158-9160	IN	denotes	to
T7591	9161-9166	NN	denotes	scale
T7592	9167-9170	NN	denotes	bar
T7593	9171-9185	NN	denotes	representation
T7594	9185-9186	.	denotes	.
T7619	9188-9191	NN	denotes	ATP
T7620	9192-9203	NN	denotes	measurement
T7621	9203-9339	sentence	denotes	ATP was measured by using the CellTiter-GLO® Luminescent Cell Viability kit (Promega), according to the manufacturer's recommendations.
T7622	9204-9207	NN	denotes	ATP
T7623	9212-9220	VBN	denotes	measured
T7624	9208-9211	VBD	denotes	was
T7625	9221-9223	IN	denotes	by
T7626	9224-9229	VBG	denotes	using
T7627	9230-9233	DT	denotes	the
T7628	9276-9279	NN	denotes	kit
T7629	9234-9243	NNP	denotes	CellTiter
T7630	9244-9248	NNP	denotes	GLO®
T7631	9243-9244	HYPH	denotes	-
T7632	9249-9260	JJ	denotes	Luminescent
T7633	9261-9265	NN	denotes	Cell
T7634	9266-9275	NN	denotes	Viability
T7635	9280-9281	-LRB-	denotes	(
T7636	9281-9288	NNP	denotes	Promega
T7637	9288-9289	-RRB-	denotes	)
T7638	9289-9291	,	denotes	,
T7639	9291-9300	VBG	denotes	according
T7640	9301-9303	IN	denotes	to
T7641	9304-9307	DT	denotes	the
T7642	9308-9320	NN	denotes	manufacturer
T7643	9323-9338	NNS	denotes	recommendations
T7644	9320-9322	POS	denotes	's
T7645	9338-9339	.	denotes	.
T7646	9339-9486	sentence	denotes	Briefly, cells were plated in a black opaque-walled 96 well plate (Packard Bioscience) at a density of 20000/well and incubated at 33°C overnight.
T7647	9340-9347	RB	denotes	Briefly
T7648	9360-9366	VBN	denotes	plated
T7649	9347-9349	,	denotes	,
T7650	9349-9354	NNS	denotes	cells
T7651	9355-9359	VBD	denotes	were
T7652	9367-9369	IN	denotes	in
T7653	9370-9371	DT	denotes	a
T7654	9400-9405	NN	denotes	plate
T7655	9372-9377	JJ	denotes	black
T7656	9378-9384	JJ	denotes	opaque
T7657	9385-9391	JJ	denotes	walled
T7658	9384-9385	HYPH	denotes	-
T7659	9392-9394	CD	denotes	96
T7660	9395-9399	NN	denotes	well
T7661	9406-9407	-LRB-	denotes	(
T7662	9415-9425	NNP	denotes	Bioscience
T7663	9407-9414	NNP	denotes	Packard
T7664	9425-9426	-RRB-	denotes	)
T7665	9427-9429	IN	denotes	at
T7666	9430-9431	DT	denotes	a
T7667	9432-9439	NN	denotes	density
T7668	9440-9442	IN	denotes	of
T7669	9443-9448	CD	denotes	20000
T7670	9448-9449	SYM	denotes	/
T7671	9449-9453	NN	denotes	well
T7672	9454-9457	CC	denotes	and
T7673	9458-9467	VBN	denotes	incubated
T7674	9468-9470	IN	denotes	at
T7675	9471-9473	CD	denotes	33
T7676	9473-9475	NN	denotes	°C
T7677	9476-9485	RB	denotes	overnight
T7678	9485-9486	.	denotes	.
T7679	9486-9599	sentence	denotes	The following day, CellTiter-GLO® Reagent was added to each well and cell lysis was induced by mixing 2 minutes.
T7680	9487-9490	DT	denotes	The
T7681	9501-9504	NN	denotes	day
T7682	9491-9500	JJ	denotes	following
T7683	9533-9538	VBN	denotes	added
T7684	9504-9506	,	denotes	,
T7685	9506-9515	NNP	denotes	CellTiter
T7686	9516-9520	NNP	denotes	GLO®
T7687	9515-9516	HYPH	denotes	-
T7688	9521-9528	NN	denotes	Reagent
T7689	9529-9532	VBD	denotes	was
T7690	9539-9541	IN	denotes	to
T7691	9542-9546	DT	denotes	each
T7692	9547-9551	NN	denotes	well
T7693	9552-9555	CC	denotes	and
T7694	9556-9560	NN	denotes	cell
T7695	9561-9566	NN	denotes	lysis
T7696	9571-9578	VBN	denotes	induced
T7697	9567-9570	VBD	denotes	was
T7698	9579-9581	IN	denotes	by
T7699	9582-9588	VBG	denotes	mixing
T7700	9589-9590	CD	denotes	2
T7701	9591-9598	NNS	denotes	minutes
T7702	9598-9599	.	denotes	.
T7703	9599-9653	sentence	denotes	An ATP standard curve was prepared in the same plate.
T7704	9600-9602	DT	denotes	An
T7705	9616-9621	NN	denotes	curve
T7706	9603-9606	NN	denotes	ATP
T7707	9607-9615	JJ	denotes	standard
T7708	9626-9634	VBN	denotes	prepared
T7709	9622-9625	VBD	denotes	was
T7710	9635-9637	IN	denotes	in
T7711	9638-9641	DT	denotes	the
T7712	9647-9652	NN	denotes	plate
T7713	9642-9646	JJ	denotes	same
T7714	9652-9653	.	denotes	.
T7715	9653-9863	sentence	denotes	Before recording luminescence with a microplate luminometer (MicroLumat Plus LB 96V, Berthold Techonologies), the plate was dark adapted for 10 minutes at room temperature to stabilize the luminescence signal.
T7716	9654-9660	IN	denotes	Before
T7717	9783-9790	VBN	denotes	adapted
T7718	9661-9670	VBG	denotes	recording
T7719	9671-9683	NN	denotes	luminescence
T7720	9684-9688	IN	denotes	with
T7721	9689-9690	DT	denotes	a
T7722	9702-9713	NN	denotes	luminometer
T7723	9691-9701	NN	denotes	microplate
T7724	9714-9715	-LRB-	denotes	(
T7725	9715-9725	NNP	denotes	MicroLumat
T7726	9734-9737	NNP	denotes	96V
T7727	9726-9730	NNP	denotes	Plus
T7728	9731-9733	NNP	denotes	LB
T7729	9737-9739	,	denotes	,
T7730	9748-9761	NNP	denotes	Techonologies
T7731	9739-9747	NNP	denotes	Berthold
T7732	9761-9762	-RRB-	denotes	)
T7733	9762-9764	,	denotes	,
T7734	9764-9767	DT	denotes	the
T7735	9768-9773	NN	denotes	plate
T7736	9774-9777	VBD	denotes	was
T7737	9778-9782	NN	denotes	dark
T7738	9791-9794	IN	denotes	for
T7739	9795-9797	CD	denotes	10
T7740	9798-9805	NNS	denotes	minutes
T7741	9806-9808	IN	denotes	at
T7742	9809-9813	NN	denotes	room
T7743	9814-9825	NN	denotes	temperature
T7744	9826-9828	TO	denotes	to
T7745	9829-9838	VB	denotes	stabilize
T7746	9839-9842	DT	denotes	the
T7747	9856-9862	NN	denotes	signal
T7748	9843-9855	NN	denotes	luminescence
T7749	9862-9863	.	denotes	.
T7780	9865-9873	NN	denotes	Hydrogen
T7781	9874-9882	NN	denotes	peroxide
T7782	9883-9892	NN	denotes	treatment
T7783	9893-9898	NN	denotes	assay
T7784	9898-10001	sentence	denotes	Cells were plated at a density of 10,000 cells/well in 96-well plates and incubated at 33°C overnight.
T7785	9899-9904	NNS	denotes	Cells
T7786	9910-9916	VBN	denotes	plated
T7787	9905-9909	VBD	denotes	were
T7788	9917-9919	IN	denotes	at
T7789	9920-9921	DT	denotes	a
T7790	9922-9929	NN	denotes	density
T7791	9930-9932	IN	denotes	of
T7792	9933-9939	CD	denotes	10,000
T7793	9940-9945	NNS	denotes	cells
T7794	9945-9946	SYM	denotes	/
T7795	9946-9950	NN	denotes	well
T7796	9951-9953	IN	denotes	in
T7797	9954-9956	CD	denotes	96
T7798	9957-9961	NN	denotes	well
T7799	9956-9957	HYPH	denotes	-
T7800	9962-9968	NNS	denotes	plates
T7801	9969-9972	CC	denotes	and
T7802	9973-9982	VBN	denotes	incubated
T7803	9983-9985	IN	denotes	at
T7804	9986-9988	CD	denotes	33
T7805	9988-9990	NN	denotes	°C
T7806	9991-10000	RB	denotes	overnight
T7807	10000-10001	.	denotes	.
T7808	10001-10115	sentence	denotes	The following day, fresh media containing varying concentrations of hydrogen peroxide was dispersed to each well.
T7809	10002-10005	DT	denotes	The
T7810	10016-10019	NN	denotes	day
T7811	10006-10015	VBG	denotes	following
T7812	10092-10101	VBN	denotes	dispersed
T7813	10019-10021	,	denotes	,
T7814	10021-10026	JJ	denotes	fresh
T7815	10027-10032	NNS	denotes	media
T7816	10033-10043	VBG	denotes	containing
T7817	10044-10051	VBG	denotes	varying
T7818	10052-10066	NNS	denotes	concentrations
T7819	10067-10069	IN	denotes	of
T7820	10070-10078	NN	denotes	hydrogen
T7821	10079-10087	NN	denotes	peroxide
T7822	10088-10091	VBD	denotes	was
T7823	10102-10104	IN	denotes	to
T7824	10105-10109	DT	denotes	each
T7825	10110-10114	NN	denotes	well
T7826	10114-10115	.	denotes	.
T7827	10115-10355	sentence	denotes	Cells were incubated in the presence of hydrogen peroxide for 24 hours, at 33°C, and viability was measured using the CellTiter-96® AQueous Non-Radioactive Cell Proliferation Assay (Promega), according to the manufacturer's specifications.
T7828	10116-10121	NNS	denotes	Cells
T7829	10127-10136	VBN	denotes	incubated
T7830	10122-10126	VBD	denotes	were
T7831	10137-10139	IN	denotes	in
T7832	10140-10143	DT	denotes	the
T7833	10144-10152	NN	denotes	presence
T7834	10153-10155	IN	denotes	of
T7835	10156-10164	NN	denotes	hydrogen
T7836	10165-10173	NN	denotes	peroxide
T7837	10174-10177	IN	denotes	for
T7838	10178-10180	CD	denotes	24
T7839	10181-10186	NNS	denotes	hours
T7840	10186-10188	,	denotes	,
T7841	10188-10190	IN	denotes	at
T7842	10191-10193	CD	denotes	33
T7843	10193-10195	NN	denotes	°C
T7844	10195-10197	,	denotes	,
T7845	10197-10200	CC	denotes	and
T7846	10201-10210	NN	denotes	viability
T7847	10215-10223	VBN	denotes	measured
T7848	10211-10214	VBD	denotes	was
T7849	10224-10229	VBG	denotes	using
T7850	10230-10233	DT	denotes	the
T7851	10291-10296	NN	denotes	Assay
T7852	10234-10243	NNP	denotes	CellTiter
T7853	10244-10247	NN	denotes	96®
T7854	10243-10244	HYPH	denotes	-
T7855	10248-10255	JJ	denotes	AQueous
T7856	10256-10271	JJ	denotes	Non-Radioactive
T7857	10272-10276	NN	denotes	Cell
T7858	10277-10290	NN	denotes	Proliferation
T7859	10297-10298	-LRB-	denotes	(
T7860	10298-10305	NNP	denotes	Promega
T7861	10305-10306	-RRB-	denotes	)
T7862	10306-10308	,	denotes	,
T7863	10308-10317	VBG	denotes	according
T7864	10318-10320	IN	denotes	to
T7865	10321-10324	DT	denotes	the
T7866	10325-10337	NN	denotes	manufacturer
T7867	10340-10354	NNS	denotes	specifications
T7868	10337-10339	POS	denotes	's
T7869	10354-10355	.	denotes	.
R4100	T6713	T6712	nummod	7.4,pH
R4101	T6714	T6706	punct	", ",formaldehyde
R4102	T6715	T6706	prep	for,formaldehyde
R4103	T6716	T6717	nummod	20,minutes
R4104	T6717	T6715	pobj	minutes,for
R4105	T6718	T6702	punct	", ",with
R4106	T6719	T6702	cc	or,with
R4108	T6721	T6722	npadvmod	ice,cold
R4109	T6722	T6724	amod	cold,acetone
R4110	T6723	T6722	punct	-,cold
R4111	T6724	T6720	pobj	acetone,with
R4112	T6725	T6724	compound	methanol,acetone
R4113	T6726	T6724	punct	/,acetone
R4114	T6727	T6728	punct	(,1
R4115	T6728	T6724	parataxis	1,acetone
R4116	T6729	T6730	punct	:,1
R4117	T6730	T6728	prep	1,1
R4252	T6871	T6870	compound	mounting,medium
R4253	T6872	T6873	punct	(,Laboratories
R4254	T6873	T6870	parataxis	Laboratories,medium
R4255	T6874	T6873	compound	Vector,Laboratories
R4256	T6875	T6873	punct	),Laboratories
R4257	T6876	T6862	cc	and,coverslipped
R4258	T6877	T6862	conj	analyzed,coverslipped
R4259	T6878	T6877	prep	on,analyzed
R4260	T6879	T6880	det	a,microscope
R4261	T6880	T6878	pobj	microscope,on
R4262	T6881	T6882	nmod	BioRad,Radiance
R4263	T6882	T6880	nmod	Radiance,microscope
R4264	T6883	T6882	nummod	2100,Radiance
R3966	T6475	T6448	conj	processed,fixed
R3967	T6476	T6475	prep	for,processed
R3968	T6477	T6476	pobj	autofluorescence,for
R3969	T6478	T6477	punct	/,autofluorescence
R3970	T6479	T6480	compound	subunit,c
R3971	T6480	T6481	compound	c,immunostaining
R3972	T6481	T6477	appos	immunostaining,autofluorescence
R3973	T6482	T6448	punct	", ",fixed
R3974	T6483	T6448	cc	or,fixed
R3975	T6484	T6485	compound	cell,pellets
R3976	T6485	T6486	nsubjpass	pellets,collected
R3977	T6486	T6448	conj	collected,fixed
R3978	T6487	T6486	auxpass	were,collected
R3979	T6488	T6486	prep	for,collected
R3980	T6489	T6490	amod	total,extraction
R3981	T6490	T6488	pobj	extraction,for
R3982	T6491	T6490	compound	protein,extraction
R3983	T6492	T6486	punct	.,collected
R3984	T6494	T6495	advmod	Alternatively,fixed
R3985	T6496	T6495	punct	", ",fixed
R3986	T6497	T6495	nsubjpass	cells,fixed
R3987	T6498	T6495	auxpass	were,fixed
R3988	T6499	T6495	prep	with,fixed
R3989	T6500	T6501	nummod	2.5,%
R3990	T6501	T6502	compound	%,glutaraldehyde
R3991	T6502	T6499	pobj	glutaraldehyde,with
R3992	T6503	T6502	punct	/,glutaraldehyde
R3993	T6504	T6505	nummod	2,%
R3994	T6505	T6506	compound	%,paraformaldehyde
R3995	T6506	T6502	appos	paraformaldehyde,glutaraldehyde
R3996	T6507	T6495	prep	in,fixed
R3997	T6508	T6509	nummod	0.1,M
R3998	T6509	T6510	compound	M,buffer
R3999	T6510	T6507	pobj	buffer,in
R4000	T6511	T6510	compound	cacodylate,buffer
R4001	T6512	T6510	punct	", ",buffer
R4050	T6658	T6659	nmod	Immunostaining,analysis
R4051	T6660	T6658	cc	and,Immunostaining
R4052	T6661	T6658	conj	Immunoblot,Immunostaining
R4053	T6663	T6664	prep	For,seeded
R4054	T6665	T6663	pobj	immunostaining,For
R4055	T6666	T6664	punct	", ",seeded
R4056	T6667	T6664	nsubj	cells,seeded
R4057	T6668	T6664	aux	were,seeded
R4058	T6669	T6664	prep	at,seeded
R4059	T6670	T6671	det	a,density
R4060	T6671	T6669	pobj	density,at
R4061	T6672	T6671	prep	of,density
R4062	T6673	T6674	quantmod	3,5
R4063	T6674	T6676	quantmod	5,104
R4064	T6675	T6674	punct	–,5
R4065	T6676	T6678	nummod	104,cells
R4066	T6677	T6676	punct	×,104
R4067	T6678	T6672	pobj	cells,of
R4068	T6679	T6678	prep	per,cells
R4069	T6680	T6679	pobj	well,per
R4070	T6681	T6664	prep	in,seeded
R4071	T6682	T6683	nummod	4,well
R4072	T6683	T6685	compound	well,slides
R4073	T6684	T6683	punct	-,well
R4074	T6685	T6681	pobj	slides,in
R4075	T6686	T6685	compound	chamber,slides
R4076	T6687	T6685	punct	-,slides
R4077	T6688	T6664	cc	and,seeded
R4078	T6689	T6664	conj	grown,seeded
R4079	T6690	T6689	advmod	overnight,grown
R4080	T6691	T6689	prep	at,grown
R4081	T6692	T6693	nummod	33,°C
R4082	T6693	T6691	pobj	°C,at
R4083	T6694	T6664	punct	", ",seeded
R4084	T6695	T6696	mark	unless,indicated
R4085	T6696	T6664	advcl	indicated,seeded
R4086	T6697	T6696	advmod	otherwise,indicated
R4087	T6698	T6664	punct	.,seeded
R4088	T6700	T6701	nsubj	Fixation,was
R4089	T6702	T6701	prep	with,was
R4090	T6703	T6704	npadvmod	ice,cold
R4091	T6704	T6706	amod	cold,formaldehyde
R4092	T6705	T6704	punct	-,cold
R4093	T6706	T6702	pobj	formaldehyde,with
R4094	T6707	T6708	nummod	4,%
R4095	T6708	T6706	compound	%,formaldehyde
R4096	T6709	T6706	prep	in,formaldehyde
R4097	T6710	T6709	pobj	PBS,in
R4098	T6711	T6710	punct	", ",PBS
R4099	T6712	T6710	npadvmod	pH,PBS
R4107	T6720	T6702	conj	with,with
R4118	T6731	T6728	punct	),1
R4119	T6732	T6724	prep	for,acetone
R4120	T6733	T6734	nummod	10,minutes
R4121	T6734	T6732	pobj	minutes,for
R4122	T6735	T6724	prep	at,acetone
R4123	T6736	T6737	punct	-,20
R4124	T6737	T6738	nummod	20,°C
R4125	T6738	T6735	pobj	°C,at
R4126	T6739	T6724	acl	followed,acetone
R4127	T6740	T6739	agent	by,followed
R4128	T6741	T6742	compound	air,drying
R4129	T6742	T6740	pobj	drying,by
R4130	T6743	T6742	punct	-,drying
R4131	T6744	T6745	punct	(,dependent
R4132	T6745	T6739	parataxis	dependent,followed
R4133	T6746	T6745	npadvmod	antibody,dependent
R4134	T6747	T6745	punct	-,dependent
R4135	T6748	T6745	punct	),dependent
R4136	T6749	T6701	punct	.,was
R4137	T6751	T6752	nsubjpass	Cells,washed
R4138	T6753	T6752	auxpass	were,washed
R4139	T6754	T6752	prep	with,washed
R4140	T6755	T6754	pobj	PBS,with
R4141	T6756	T6757	advmod	at,2
R4142	T6757	T6752	npadvmod	2,washed
R4143	T6758	T6757	advmod	least,2
R4144	T6759	T6757	quantmod	times,2
R4145	T6760	T6752	punct	", ",washed
R4146	T6761	T6762	nummod	5,minutes
R4147	T6762	T6752	npadvmod	minutes,washed
R4148	T6763	T6762	prep	per,minutes
R4149	T6764	T6763	pobj	wash,per
R4150	T6765	T6752	punct	", ",washed
R4151	T6766	T6752	prep	between,washed
R4152	T6767	T6766	pobj	each,between
R4153	T6768	T6767	prep	of,each
R4154	T6769	T6770	det	the,steps
R4155	T6770	T6768	pobj	steps,of
R4156	T6771	T6770	amod	following,steps
R4157	T6772	T6770	prep	of,steps
R4158	T6773	T6774	det	the,procedure
R4159	T6774	T6772	pobj	procedure,of
R4160	T6775	T6774	compound	staining,procedure
R4161	T6776	T6770	punct	: ,steps
R4162	T6777	T6778	nummod	0.1,M
R4163	T6778	T6779	compound	M,glycine
R4164	T6779	T6770	appos	glycine,steps
R4165	T6780	T6779	prep	in,glycine
R4166	T6781	T6780	pobj	PBS,in
R4167	T6782	T6779	prep	for,glycine
R4168	T6783	T6784	nummod	5,minutes
R4169	T6784	T6782	pobj	minutes,for
R4170	T6785	T6779	punct	", ",glycine
R4171	T6786	T6787	nummod	0.05,%
R4172	T6787	T6788	nmod	%,X
R4173	T6788	T6779	appos	X,glycine
R4174	T6789	T6787	cc	or,%
R4175	T6790	T6791	nummod	0.1,%
R4176	T6791	T6787	conj	%,%
R4177	T6792	T6788	punct	(,X
R4178	T6793	T6794	npadvmod	antibody,dependent
R4179	T6794	T6788	amod	dependent,X
R4180	T6795	T6794	punct	-,dependent
R4181	T6796	T6788	punct	),X
R4182	T6797	T6788	compound	Triton,X
R4183	T6798	T6788	punct	-,X
R4184	T6799	T6788	nummod	100,X
R4185	T6800	T6801	punct	(,Scientific
R4186	T6801	T6788	parataxis	Scientific,X
R4187	T6802	T6801	compound	Fisher,Scientific
R4188	T6803	T6801	punct	),Scientific
R4189	T6804	T6788	prep	in,X
R4190	T6805	T6804	pobj	PBS,in
R4191	T6806	T6788	prep	for,X
R4192	T6807	T6808	nummod	5,minutes
R4193	T6808	T6806	pobj	minutes,for
R4194	T6809	T6779	punct	", ",glycine
R4195	T6810	T6811	nummod	2,%
R4196	T6811	T6779	appos	%,glycine
R4197	T6812	T6813	amod	bovine,albumin
R4198	T6813	T6811	appos	albumin,%
R4199	T6814	T6813	compound	serum,albumin
R4200	T6815	T6813	punct	(,albumin
R4201	T6816	T6813	appos	BSA,albumin
R4202	T6817	T6811	punct	),%
R4203	T6818	T6811	prep	in,%
R4204	T6819	T6818	pobj	PBS,in
R4205	T6820	T6811	prep	for,%
R4206	T6821	T6822	nummod	30,minutes
R4207	T6822	T6820	pobj	minutes,for
R4208	T6823	T6779	punct	", ",glycine
R4209	T6824	T6825	amod	primary,antibody
R4210	T6825	T6779	appos	antibody,glycine
R4211	T6826	T6825	acl	diluted,antibody
R4212	T6827	T6826	prep	in,diluted
R4213	T6828	T6829	nummod	2,%
R4214	T6829	T6830	compound	%,PBS
R4215	T6830	T6827	pobj	PBS,in
R4216	T6831	T6830	compound	BSA,PBS
R4217	T6832	T6830	punct	/,PBS
R4218	T6833	T6826	prep	for,diluted
R4219	T6834	T6835	nummod	90,minutes
R4220	T6835	T6833	pobj	minutes,for
R4221	T6836	T6779	punct	", ",glycine
R4222	T6837	T6838	amod	secondary,antibody
R4223	T6838	T6779	appos	antibody,glycine
R4224	T6839	T6838	acl	diluted,antibody
R4225	T6840	T6839	prep	in,diluted
R4226	T6841	T6842	nummod	2,%
R4227	T6842	T6843	compound	%,PBS
R4228	T6843	T6840	pobj	PBS,in
R4229	T6844	T6843	compound	BSA,PBS
R4230	T6845	T6843	punct	/,PBS
R4231	T6846	T6839	prep	for,diluted
R4232	T6847	T6848	nummod	60,minutes
R4233	T6848	T6846	pobj	minutes,for
R4234	T6849	T6752	punct	.,washed
R4235	T6851	T6852	det	All,incubations
R4236	T6852	T6853	nsubjpass	incubations,carried
R4237	T6854	T6853	auxpass	were,carried
R4238	T6855	T6853	prt	out,carried
R4239	T6856	T6853	prep	at,carried
R4240	T6857	T6858	compound	room,temperature
R4241	T6858	T6856	pobj	temperature,at
R4242	T6859	T6853	punct	.,carried
R4243	T6861	T6862	prep	Following,coverslipped
R4244	T6863	T6864	compound	staining,procedures
R4245	T6864	T6861	pobj	procedures,Following
R4246	T6865	T6862	punct	", ",coverslipped
R4247	T6866	T6862	nsubjpass	slides,coverslipped
R4248	T6867	T6862	auxpass	were,coverslipped
R4249	T6868	T6862	prep	with,coverslipped
R4250	T6869	T6870	compound	Vectashield,medium
R4251	T6870	T6868	pobj	medium,with
R4265	T6884	T6880	amod	confocal,microscope
R4266	T6885	T6886	punct	(,Biorad
R4267	T6886	T6880	parataxis	Biorad,microscope
R4268	T6887	T6886	punct	),Biorad
R4269	T6888	T6877	punct	", ",analyzed
R4270	T6889	T6877	prep	with,analyzed
R4271	T6890	T6891	amod	identical,settings
R4272	T6891	T6889	pobj	settings,with
R4273	T6892	T6891	compound	exposure,settings
R4274	T6893	T6891	prep	for,settings
R4275	T6894	T6895	amod	wild,type
R4276	T6895	T6897	nmod	type,images
R4277	T6896	T6895	punct	-,type
R4278	T6897	T6893	pobj	images,for
R4279	T6898	T6895	cc	and,type
R4280	T6899	T6895	conj	mutant,type
R4281	T6900	T6897	amod	like,images
R4282	T6901	T6862	punct	.,coverslipped
R4283	T6903	T6904	det	All,comparisons
R4284	T6904	T6905	nsubjpass	comparisons,performed
R4285	T6906	T6904	prep	of,comparisons
R4286	T6907	T6908	amod	wild,type
R4287	T6908	T6910	nmod	type,staining
R4288	T6909	T6908	punct	-,type
R4289	T6910	T6906	pobj	staining,of
R4290	T6911	T6908	cc	and,type
R4291	T6912	T6908	conj	mutant,type
R4292	T6913	T6905	auxpass	were,performed
R4293	T6914	T6905	prep	in,performed
R4294	T6915	T6916	compound	Adobe,Photoshop
R4295	T6916	T6914	pobj	Photoshop,in
R4296	T6917	T6905	prep	with,performed
R4297	T6918	T6919	amod	identical,adjustments
R4298	T6919	T6917	pobj	adjustments,with
R4299	T6920	T6919	nmod	brightness,adjustments
R4300	T6921	T6920	cc	and,brightness
R4301	T6922	T6920	conj	contrast,brightness
R4302	T6923	T6905	punct	.,performed
R4303	T6925	T6926	amod	Total,proteins
R4304	T6926	T6927	nsubjpass	proteins,isolated
R4305	T6928	T6927	auxpass	were,isolated
R4306	T6929	T6927	prep	from,isolated
R4307	T6930	T6931	compound	cell,pellets
R4308	T6931	T6929	pobj	pellets,from
R4309	T6932	T6927	prep	by,isolated
R4310	T6933	T6932	pobj	extraction,by
R4311	T6934	T6933	prep	with,extraction
R4312	T6935	T6936	nmod	ice,Tris
R4313	T6936	T6934	pobj	Tris,with
R4314	T6937	T6935	punct	-,ice
R4315	T6938	T6935	amod	cold,ice
R4316	T6939	T6940	nummod	20,mM
R4317	T6940	T6936	compound	mM,Tris
R4318	T6941	T6936	punct	", ",Tris
R4319	T6942	T6936	npadvmod	pH,Tris
R4320	T6943	T6942	nummod	7.4,pH
R4321	T6944	T6936	punct	", ",Tris
R4322	T6945	T6946	nummod	1,%
R4323	T6946	T6947	compound	%,X
R4324	T6947	T6936	conj	X,Tris
R4325	T6948	T6947	compound	Triton,X
R4326	T6949	T6947	punct	-,X
R4327	T6950	T6947	nummod	100,X
R4328	T6951	T6952	punct	(,Roche
R4329	T6952	T6947	parataxis	Roche,X
R4330	T6953	T6954	compound	membrane,research
R4331	T6954	T6956	compound	research,grade
R4332	T6955	T6954	punct	-,research
R4333	T6956	T6952	dep	grade,Roche
R4334	T6957	T6952	punct	", ",Roche
R4335	T6958	T6952	punct	),Roche
R4336	T6959	T6947	punct	", ",X
R4337	T6960	T6947	cc	plus,X
R4338	T6961	T6962	compound	protease,inhibitors
R4339	T6962	T6947	conj	inhibitors,X
R4340	T6963	T6962	punct	(,inhibitors
R4341	T6964	T6965	amod	Complete,tablet
R4342	T6965	T6962	appos	tablet,inhibitors
R4343	T6966	T6965	compound	mini,tablet
R4344	T6967	T6965	punct	", ",tablet
R4345	T6968	T6969	nummod	0.7,μg
R4346	T6969	T6970	nmod	μg,A
R4347	T6970	T6965	appos	A,tablet
R4348	T6971	T6972	punct	/,ml
R4349	T6972	T6969	prep	ml,μg
R4350	T6973	T6970	compound	pepstatin,A
R4351	T6974	T6965	punct	", ",tablet
R4352	T6975	T6976	nummod	2,μg
R4353	T6976	T6977	nmod	μg,aprotinin
R4354	T6977	T6965	appos	aprotinin,tablet
R4355	T6978	T6979	punct	/,ml
R4356	T6979	T6976	prep	ml,μg
R4357	T6980	T6965	punct	", ",tablet
R4358	T6981	T6982	nummod	5,μg
R4359	T6982	T6983	nmod	μg,leupeptin
R4360	T6983	T6965	appos	leupeptin,tablet
R4361	T6984	T6985	punct	/,ml
R4362	T6985	T6982	prep	ml,μg
R4363	T6986	T6987	punct	[,Roche
R4364	T6987	T6983	parataxis	Roche,leupeptin
R4365	T6988	T6987	punct	],Roche
R4366	T6989	T6927	punct	),isolated
R4367	T6990	T6927	punct	.,isolated
R4368	T6992	T6993	prep	Following,centrifuged
R4369	T6994	T6992	pobj	homogenization,Following
R4370	T6995	T6994	prep	through,homogenization
R4371	T6996	T6997	det	a,needle
R4372	T6997	T6995	pobj	needle,through
R4373	T6998	T6999	nummod	25,gauge
R4374	T6999	T6997	compound	gauge,needle
R4375	T7000	T6999	punct	-,gauge
R4376	T7001	T7002	punct	(,passes
R4377	T7002	T6994	parataxis	passes,homogenization
R4378	T7003	T7004	punct	~,10
R4379	T7004	T7002	nummod	10,passes
R4380	T7005	T7002	punct	),passes
R4381	T7006	T6993	punct	", ",centrifuged
R4382	T7007	T6993	nsubjpass	extracts,centrifuged
R4383	T7008	T6993	auxpass	were,centrifuged
R4384	T7009	T6993	prep	at,centrifuged
R4385	T7010	T7011	nummod	1000,g
R4386	T7011	T7009	pobj	g,at
R4387	T7012	T7011	punct	×,g
R4388	T7013	T6993	prep	for,centrifuged
R4389	T7014	T7015	nummod	10,minutes
R4390	T7015	T7013	pobj	minutes,for
R4391	T7016	T6993	punct	", ",centrifuged
R4392	T7017	T6993	prep	at,centrifuged
R4393	T7018	T7019	nummod	4,°C
R4394	T7019	T7017	pobj	°C,at
R4395	T7020	T6993	punct	", ",centrifuged
R4396	T7021	T7022	aux	to,remove
R4397	T7022	T6993	advcl	remove,centrifuged
R4398	T7023	T7022	dobj	debris,remove
R4399	T7024	T6993	punct	.,centrifuged
R4400	T7026	T7027	advmod	Typically,separated
R4401	T7028	T7027	punct	", ",separated
R4402	T7029	T7030	quantmod	20,40
R4403	T7030	T7032	nummod	40,μg
R4404	T7031	T7030	punct	–,40
R4405	T7032	T7027	nsubjpass	μg,separated
R4406	T7033	T7032	prep	of,μg
R4407	T7034	T7033	pobj	protein,of
R4408	T7035	T7034	punct	(,protein
R4409	T7036	T7034	acl	determined,protein
R4410	T7037	T7036	agent	by,determined
R4411	T7038	T7039	compound	Bio,rad
R4412	T7039	T7041	compound	rad,Assay
R4413	T7040	T7039	punct	-,rad
R4414	T7041	T7037	pobj	Assay,by
R4415	T7042	T7041	compound	Dc,Assay
R4416	T7043	T7041	compound	Protein,Assay
R4417	T7044	T7027	punct	),separated
R4418	T7045	T7027	auxpass	was,separated
R4419	T7046	T7027	prep	by,separated
R4420	T7047	T7048	compound	SDS,PAGE
R4421	T7048	T7046	pobj	PAGE,by
R4422	T7049	T7048	punct	-,PAGE
R4423	T7050	T7027	punct	", ",separated
R4424	T7051	T7027	prep	for,separated
R4425	T7052	T7053	amod	subsequent,immunoblotting
R4426	T7053	T7051	pobj	immunoblotting,for
R4427	T7054	T7027	punct	", ",separated
R4428	T7055	T7056	mark	as,described
R4429	T7056	T7027	advcl	described,separated
R4430	T7057	T7058	punct	[,12
R4431	T7058	T7027	parataxis	12,separated
R4432	T7059	T7058	punct	],12
R4433	T7060	T7027	punct	.,separated
R4434	T7062	T7063	nummod	16.5,%
R4435	T7063	T7064	compound	%,gels
R4436	T7064	T7071	nsubjpass	gels,used
R4437	T7065	T7066	compound	tris,tricine
R4438	T7066	T7064	compound	tricine,gels
R4439	T7067	T7066	punct	-,tricine
R4440	T7068	T7069	compound	SDS,PAGE
R4441	T7069	T7064	compound	PAGE,gels
R4442	T7070	T7069	punct	-,PAGE
R4443	T7072	T7071	auxpass	were,used
R4444	T7073	T7071	prep	for,used
R4445	T7074	T7075	compound	subunit,c
R4446	T7075	T7076	compound	c,experiments
R4447	T7076	T7073	pobj	experiments,for
R4448	T7077	T7076	compound	immunoblotting,experiments
R4449	T7078	T7071	punct	.,used
R4453	T7121	T7122	nummod	100,mm
R4454	T7122	T7123	compound	mm,dishes
R4455	T7123	T7126	nsubjpass	dishes,washed
R4456	T7124	T7125	compound	tissue,culture
R4457	T7125	T7123	compound	culture,dishes
R4458	T7127	T7123	punct	", ",dishes
R4459	T7128	T7129	dep	which,were
R4460	T7129	T7123	relcl	were,dishes
R4461	T7130	T7131	advmod	approximately,90
R4462	T7131	T7134	nummod	90,%
R4463	T7132	T7131	quantmod	80,90
R4464	T7133	T7131	punct	–,90
R4465	T7134	T7135	npadvmod	%,confluent
R4466	T7135	T7129	acomp	confluent,were
R4467	T7136	T7126	punct	", ",washed
R4468	T7137	T7126	auxpass	were,washed
R4469	T7138	T7126	advmod	briefly,washed
R4470	T7139	T7126	prep	with,washed
R4471	T7140	T7141	npadvmod	ice,cold
R4472	T7141	T7143	amod	cold,PBS
R4473	T7142	T7141	punct	-,cold
R4474	T7143	T7139	pobj	PBS,with
R4475	T7144	T7126	punct	", ",washed
R4476	T7145	T7126	cc	and,washed
R4477	T7146	T7147	amod	total,extracts
R4478	T7147	T7149	nsubjpass	extracts,isolated
R4479	T7148	T7147	compound	protein,extracts
R4480	T7149	T7126	conj	isolated,washed
R4481	T7150	T7149	auxpass	were,isolated
R4482	T7151	T7149	prep	by,isolated
R4483	T7152	T7151	pcomp	scraping,by
R4484	T7153	T7152	dobj	cells,scraping
R4485	T7154	T7152	prep	into,scraping
R4486	T7155	T7156	nummod	10,mM
R4487	T7156	T7157	compound	mM,Tris
R4488	T7157	T7154	pobj	Tris,into
R4489	T7158	T7157	punct	", ",Tris
R4490	T7159	T7157	npadvmod	pH,Tris
R4491	T7160	T7159	nummod	7.4,pH
R4512	T7183	T7182	auxpass	was,centrifuged
R4513	T7184	T7182	prep	at,centrifuged
R4514	T7185	T7186	nummod	"14,000",g
R4515	T7186	T7184	pobj	g,at
R4516	T7187	T7182	punct	", ",centrifuged
R4517	T7188	T7189	det	the,supernatant
R4518	T7189	T7190	nsubjpass	supernatant,isolated
R4520	T7191	T7190	auxpass	was,isolated
R4523	T7194	T7195	compound	protein,concentration
R4524	T7195	T7196	nsubjpass	concentration,determined
R4525	T7196	T7190	conj	determined,isolated
R4526	T7197	T7196	auxpass	was,determined
R4527	T7198	T7196	advcl	using,determined
R4528	T7199	T7200	det	the,Assay
R4529	T7200	T7198	dobj	Assay,using
R4530	T7201	T7202	compound	Bio,rad
R4531	T7202	T7200	compound	rad,Assay
R4532	T7203	T7202	punct	-,rad
R4533	T7204	T7200	compound	Dc,Assay
R4534	T7205	T7200	compound	Protein,Assay
R4535	T7206	T7196	punct	.,determined
R4536	T7208	T7209	quantmod	50,70
R4537	T7209	T7211	nummod	70,μg
R4538	T7210	T7209	punct	–,70
R4539	T7211	T7212	nsubjpass	μg,used
R4540	T7213	T7211	prep	of,μg
R4541	T7214	T7215	amod	total,extract
R4542	T7215	T7213	pobj	extract,of
R4543	T7216	T7215	compound	protein,extract
R4544	T7217	T7212	auxpass	were,used
R4545	T7218	T7219	aux	to,measure
R4546	T7219	T7212	advcl	measure,used
R4547	T7220	T7221	compound	cathepsin,D
R4548	T7221	T7222	compound	D,activity
R4549	T7222	T7219	dobj	activity,measure
R4550	T7223	T7219	advcl	using,measure
R4551	T7224	T7225	det	the,Kit
R4552	T7225	T7223	dobj	Kit,using
R4553	T7226	T7227	amod	Fluorogenic,D
R4554	T7227	T7225	compound	D,Kit
R4555	T7228	T7227	nmod	Innozyme,D
R4556	T7229	T7227	punct	™,D
R4557	T7230	T7227	compound	Cathepsin,D
R4558	T7231	T7225	compound	Immunocapture,Kit
R4559	T7232	T7225	compound	Activity,Kit
R4560	T7233	T7225	compound	Assay,Kit
R4561	T7234	T7235	punct	(,Biosciences
R4562	T7235	T7225	parataxis	Biosciences,Kit
R4563	T7236	T7235	compound	EMD,Biosciences
R4564	T7237	T7235	punct	),Biosciences
R4565	T7238	T7223	prep	according,using
R4566	T7239	T7238	prep	to,according
R4567	T7240	T7241	det	the,manufacturer
R4568	T7241	T7242	poss	manufacturer,recommendations
R4569	T7242	T7239	pobj	recommendations,to
R4570	T7243	T7241	case	's,manufacturer
R4571	T7244	T7212	punct	.,used
R4572	T7246	T7247	amod	Relative,fluorescence
R4573	T7247	T7248	nsubjpass	fluorescence,measured
R4574	T7249	T7248	auxpass	was,measured
R4575	T7250	T7248	advcl	using,measured
R4576	T7251	T7252	det	an,reader
R4577	T7252	T7250	dobj	reader,using
R4578	T7253	T7254	compound	Analyst,AD
R4579	T7254	T7252	compound	AD,reader
R4580	T7255	T7252	compound	plate,reader
R4581	T7256	T7257	punct	(,Devices
R4582	T7257	T7252	parataxis	Devices,reader
R4583	T7258	T7257	amod	Molecular,Devices
R4584	T7259	T7257	punct	),Devices
R4585	T7260	T7250	prep	with,using
R4586	T7261	T7262	det	the,filters
R4587	T7262	T7260	pobj	filters,with
R4588	T7263	T7262	amod	following,filters
R4589	T7264	T7262	cc	and,filters
R4590	T7265	T7262	conj	settings,filters
R4591	T7266	T7248	punct	: ,measured
R4592	T7267	T7268	compound	excitation,filter
R4593	T7268	T7269	dep	filter,35
R4602	T7277	T7276	punct	", ",20
R4603	T7278	T7276	nummod	400,20
R4604	T7279	T7276	punct	-,20
R4605	T7280	T7276	punct	", ",20
R4606	T7281	T7282	compound	Flash,lamp
R4607	T7282	T7276	conj	lamp,20
R4608	T7283	T7282	prep	with,lamp
R4609	T7284	T7285	nummod	100,readings
R4610	T7285	T7283	pobj	readings,with
R4611	T7286	T7287	punct	/,well
R4612	T7287	T7285	prep	well,readings
R4613	T7288	T7282	punct	", ",lamp
R4614	T7289	T7290	nummod	100,ms
R4615	T7290	T7282	conj	ms,lamp
R4616	T7291	T7290	prep	between,ms
R4617	T7292	T7291	pobj	readings,between
R4618	T7293	T7290	punct	", ",ms
R4619	T7294	T7290	cc	and,ms
R4620	T7295	T7296	nummod	"100,000",μs
R4621	T7296	T7297	compound	μs,time
R4622	T7297	T7290	conj	time,ms
R4623	T7298	T7297	compound	integration,time
R4624	T7299	T7248	punct	.,measured
R4625	T7337	T7338	amod	Lysosomal,staining
R4626	T7339	T7338	cc	and,staining
R4627	T7340	T7341	amod	endocytic,uptake
R4628	T7341	T7338	conj	uptake,staining
R4629	T7343	T7344	nsubjpass	Cells,seeded
R4630	T7345	T7344	auxpass	were,seeded
R4631	T7346	T7344	prep	at,seeded
R4632	T7347	T7348	det	a,density
R4633	T7348	T7346	pobj	density,at
R4634	T7349	T7348	prep	of,density
R4635	T7350	T7351	quantmod	3,5
R4636	T7351	T7353	quantmod	5,104
R4637	T7352	T7351	punct	–,5
R4638	T7353	T7355	nummod	104,cells
R4639	T7354	T7353	punct	×,104
R4640	T7355	T7349	pobj	cells,of
R4641	T7356	T7355	prep	per,cells
R4642	T7357	T7356	pobj	well,per
R4643	T7358	T7344	prep	in,seeded
R4644	T7359	T7360	nummod	4,well
R4645	T7360	T7362	compound	well,slides
R4646	T7361	T7360	punct	-,well
R4647	T7362	T7358	pobj	slides,in
R4648	T7363	T7362	compound	chamber,slides
R4649	T7364	T7362	punct	-,slides
R4650	T7365	T7344	cc	and,seeded
R4651	T7366	T7344	conj	grown,seeded
R4652	T7367	T7366	advmod	overnight,grown
R4653	T7368	T7366	prep	at,grown
R4654	T7369	T7370	nummod	33,°C
R4655	T7370	T7368	pobj	°C,at
R4656	T7371	T7344	punct	.,seeded
R4657	T7373	T7374	compound	Growth,media
R4658	T7374	T7375	nsubjpass	media,exchanged
R4659	T7376	T7375	auxpass	was,exchanged
R4660	T7377	T7375	prep	for,exchanged
R4661	T7378	T7379	amod	fresh,media
R4662	T7379	T7377	pobj	media,for
R4663	T7380	T7379	punct	", ",media
R4664	T7381	T7379	amod	pre-warmed,media
R4665	T7382	T7379	compound	growth,media
R4666	T7383	T7379	acl	containing,media
R4667	T7384	T7385	nummod	500,nM
R4668	T7385	T7386	compound	nM,Lysotracker
R4669	T7386	T7383	dobj	Lysotracker,containing
R4670	T7387	T7386	cc	or,Lysotracker
R4671	T7388	T7389	nummod	1,mg
R4672	T7389	T7390	nmod	mg,FITC
R4673	T7390	T7386	conj	FITC,Lysotracker
R4674	T7391	T7392	punct	/,ml
R4675	T7392	T7389	prep	ml,mg
R4676	T7393	T7390	compound	dextran,FITC
R4677	T7394	T7390	punct	-,FITC
R4678	T7395	T7375	punct	", ",exchanged
R4679	T7396	T7375	cc	and,exchanged
R4680	T7397	T7398	nsubjpass	cells,incubated
R4681	T7398	T7375	conj	incubated,exchanged
R4682	T7399	T7398	auxpass	were,incubated
R4683	T7400	T7398	prep	at,incubated
R4684	T7401	T7402	nummod	33,°C
R4685	T7402	T7400	pobj	°C,at
R4686	T7403	T7398	prep	for,incubated
R4687	T7404	T7405	nummod	45,minutes
R4688	T7405	T7403	pobj	minutes,for
R4689	T7406	T7405	cc	or,minutes
R4690	T7407	T7408	nummod	15,minutes
R4691	T7408	T7405	conj	minutes,minutes
R4692	T7409	T7398	punct	", ",incubated
R4693	T7410	T7398	advmod	respectively,incubated
R4694	T7411	T7398	punct	.,incubated
R4695	T7413	T7414	prep	Following,placed
R4696	T7415	T7413	pobj	labeling,Following
R4697	T7416	T7414	punct	", ",placed
R4698	T7417	T7414	nsubjpass	cells,placed
R4699	T7418	T7414	auxpass	were,placed
R4700	T7419	T7414	advmod	immediately,placed
R4701	T7420	T7414	prep	on,placed
R4702	T7421	T7420	pobj	ice,on
R4703	T7422	T7414	cc	and,placed
R4704	T7423	T7414	conj	washed,placed
R4705	T7424	T7423	prep	for,washed
R4706	T7425	T7426	nummod	10,minutes
R4707	T7426	T7424	pobj	minutes,for
R4708	T7427	T7423	prep	in,washed
R4709	T7428	T7429	npadvmod	ice,cold
R4710	T7429	T7431	amod	cold,media
R4711	T7430	T7429	punct	-,cold
R4712	T7431	T7427	pobj	media,in
R4713	T7432	T7433	npadvmod	dye,free
R4714	T7433	T7431	amod	free,media
R4715	T7434	T7433	punct	-,free
R4716	T7435	T7423	punct	", ",washed
R4717	T7436	T7423	cc	and,washed
R4798	T7536	T7537	quantmod	2.5,4
R4718	T7437	T7423	conj	fixed,washed
R4719	T7438	T7437	prep	with,fixed
R4720	T7439	T7440	nummod	4,%
R4721	T7440	T7441	compound	%,formaldehyde
R4722	T7441	T7438	pobj	formaldehyde,with
R4723	T7442	T7441	prep	in,formaldehyde
R4724	T7443	T7442	pobj	PBS,in
R4725	T7444	T7437	punct	", ",fixed
R4726	T7445	T7437	prep	for,fixed
R4727	T7446	T7447	nummod	20,minutes
R4728	T7447	T7445	pobj	minutes,for
R4729	T7448	T7437	prep	on,fixed
R4730	T7449	T7448	pobj	ice,on
R4731	T7450	T7414	punct	.,placed
R4732	T7452	T7453	nsubjpass	Chambers,removed
R4733	T7454	T7453	auxpass	were,removed
R4734	T7455	T7453	cc	and,removed
R4735	T7456	T7457	nsubjpass	slides,coverslipped
R4736	T7457	T7453	conj	coverslipped,removed
R4737	T7458	T7457	auxpass	were,coverslipped
R4738	T7459	T7457	prep	with,coverslipped
R4739	T7460	T7461	compound	Vectashield,media
R4740	T7461	T7459	pobj	media,with
R4741	T7462	T7461	compound	mounting,media
R4742	T7463	T7461	prep	for,media
R4743	T7464	T7465	amod	confocal,microscopy
R4744	T7465	T7466	compound	microscopy,analysis
R4745	T7466	T7463	pobj	analysis,for
R4746	T7467	T7457	punct	", ",coverslipped
R4747	T7468	T7469	mark	as,described
R4748	T7469	T7457	advcl	described,coverslipped
R4749	T7470	T7469	advmod	above,described
R4750	T7471	T7457	punct	.,coverslipped
R4751	T7484	T7485	amod	Morphometric,analysis
R4752	T7486	T7485	prep	of,analysis
R4753	T7487	T7486	pobj	mitochondria,of
R4754	T7489	T7490	compound	TEM,photomicrographs
R4755	T7490	T7491	nsubjpass	photomicrographs,taken
R4756	T7492	T7493	punct	(,magnification
R4757	T7493	T7490	parataxis	magnification,photomicrographs
R4758	T7494	T7495	quantmod	"10,000","40,000"
R4759	T7495	T7493	nummod	"40,000",magnification
R4760	T7496	T7494	punct	×,"10,000"
R4761	T7497	T7495	punct	–,"40,000"
R4762	T7498	T7495	punct	×,"40,000"
R4763	T7499	T7493	punct	),magnification
R4764	T7500	T7491	auxpass	were,taken
R4765	T7501	T7491	prep	from,taken
R4766	T7502	T7503	amod	random,fields
R4767	T7503	T7501	pobj	fields,from
R4768	T7504	T7503	compound	grid,fields
R4769	T7505	T7491	punct	.,taken
R4770	T7507	T7508	prep	For,measured
R4771	T7509	T7510	compound	length,measurements
R4772	T7510	T7507	pobj	measurements,For
R4773	T7511	T7508	punct	", ",measured
R4774	T7512	T7513	det	the,side
R4775	T7513	T7508	nsubjpass	side,measured
R4776	T7514	T7513	amod	longest,side
R4777	T7515	T7513	prep	of,side
R4778	T7516	T7517	det	each,mitochondria
R4779	T7517	T7515	pobj	mitochondria,of
R4780	T7518	T7508	auxpass	was,measured
R4781	T7519	T7508	prep	in,measured
R4782	T7520	T7519	pobj	centimeters,in
R4783	T7521	T7508	punct	", ",measured
R4784	T7522	T7508	cc	and,measured
R4785	T7523	T7524	prep	along,taken
R4786	T7524	T7508	conj	taken,measured
R4787	T7525	T7526	det	the,length
R4788	T7526	T7523	pobj	length,along
R4789	T7527	T7526	prep	of,length
R4790	T7528	T7529	det	the,mitochondria
R4791	T7529	T7527	pobj	mitochondria,of
R4792	T7530	T7524	punct	", ",taken
R4793	T7531	T7532	compound	width,measurements
R4794	T7532	T7524	nsubjpass	measurements,taken
R4795	T7533	T7524	auxpass	were,taken
R4796	T7534	T7535	det	every,mm
R4797	T7535	T7524	npadvmod	mm,taken
R4799	T7537	T7535	nummod	4,mm
R4800	T7538	T7537	punct	–,4
R4801	T7539	T7535	punct	(,mm
R4802	T7540	T7535	amod	dependent,mm
R4803	T7541	T7540	prep	on,dependent
R4804	T7542	T7543	det	the,magnification
R4805	T7543	T7541	pobj	magnification,on
R4806	T7544	T7543	prep	of,magnification
R4807	T7545	T7546	det	the,image
R4808	T7546	T7544	pobj	image,of
R4809	T7547	T7546	compound	micrograph,image
R4810	T7548	T7524	punct	),taken
R4811	T7549	T7524	punct	.,taken
R4812	T7551	T7552	prep	Following,normalized
R4813	T7553	T7551	pobj	measurement,Following
R4814	T7554	T7552	punct	", ",normalized
R4815	T7555	T7556	det	all,numbers
R4816	T7556	T7552	nsubjpass	numbers,normalized
R4817	T7557	T7552	auxpass	were,normalized
R4818	T7558	T7559	aux	to,reflect
R4819	T7559	T7552	advcl	reflect,normalized
R4820	T7560	T7561	nummod	one,magnification
R4821	T7561	T7559	dobj	magnification,reflect
R4822	T7562	T7552	cc	and,normalized
R4823	T7563	T7564	nsubjpass	data,analyzed
R4824	T7564	T7552	conj	analyzed,normalized
R4825	T7565	T7564	auxpass	was,analyzed
R4826	T7566	T7564	advcl	using,analyzed
R4827	T7567	T7568	compound	Microsoft,Excel
R4828	T7568	T7569	compound	Excel,software
R4829	T7569	T7566	dobj	software,using
R4830	T7570	T7564	punct	.,analyzed
R4831	T7572	T7573	aux	To,ascertain
R4832	T7573	T7574	advcl	ascertain,converted
R4833	T7575	T7576	amod	unmagnified,size
R4834	T7576	T7573	dobj	size,ascertain
R4835	T7577	T7576	amod	mitochondrial,size
R4836	T7578	T7574	punct	", ",converted
R4837	T7579	T7580	amod	final,data
R4838	T7580	T7574	nsubjpass	data,converted
R4839	T7581	T7580	compound	measurement,data
R4840	T7582	T7580	punct	", ",data
R4841	T7583	T7580	prep	in,data
R4842	T7584	T7583	pobj	centimeters,in
R4843	T7585	T7574	punct	", ",converted
R4844	T7586	T7574	auxpass	was,converted
R4845	T7587	T7574	prep	to,converted
R4846	T7588	T7587	pobj	nanometers,to
R4847	T7589	T7574	prep	according,converted
R4848	T7590	T7589	prep	to,according
R4849	T7591	T7592	compound	scale,bar
R4850	T7592	T7593	compound	bar,representation
R4851	T7593	T7590	pobj	representation,to
R4852	T7594	T7574	punct	.,converted
R4853	T7619	T7620	compound	ATP,measurement
R4854	T7622	T7623	nsubjpass	ATP,measured
R4855	T7624	T7623	auxpass	was,measured
R4856	T7625	T7623	prep	by,measured
R4857	T7626	T7625	pcomp	using,by
R4858	T7627	T7628	det	the,kit
R4859	T7628	T7626	dobj	kit,using
R4860	T7629	T7630	nmod	CellTiter,GLO®
R4861	T7630	T7628	nmod	GLO®,kit
R4862	T7631	T7630	punct	-,GLO®
R4863	T7632	T7628	amod	Luminescent,kit
R4864	T7633	T7634	compound	Cell,Viability
R4865	T7634	T7628	compound	Viability,kit
R4866	T7635	T7636	punct	(,Promega
R4867	T7636	T7628	parataxis	Promega,kit
R4868	T7637	T7636	punct	),Promega
R4869	T7638	T7623	punct	", ",measured
R4870	T7639	T7623	prep	according,measured
R4871	T7640	T7639	prep	to,according
R4872	T7641	T7642	det	the,manufacturer
R4873	T7642	T7643	poss	manufacturer,recommendations
R4874	T7643	T7640	pobj	recommendations,to
R4875	T7644	T7642	case	's,manufacturer
R4876	T7645	T7623	punct	.,measured
R4877	T7647	T7648	advmod	Briefly,plated
R4878	T7649	T7648	punct	", ",plated
R4879	T7650	T7648	nsubjpass	cells,plated
R4880	T7651	T7648	auxpass	were,plated
R4881	T7652	T7648	prep	in,plated
R4882	T7653	T7654	det	a,plate
R4883	T7654	T7652	pobj	plate,in
R4884	T7655	T7654	amod	black,plate
R4885	T7656	T7657	amod	opaque,walled
R4886	T7657	T7654	amod	walled,plate
R4887	T7658	T7657	punct	-,walled
R4888	T7659	T7660	nummod	96,well
R4889	T7660	T7654	compound	well,plate
R4890	T7661	T7662	punct	(,Bioscience
R4891	T7662	T7654	parataxis	Bioscience,plate
R4892	T7663	T7662	compound	Packard,Bioscience
R4893	T7664	T7662	punct	),Bioscience
R4894	T7665	T7648	prep	at,plated
R4895	T7666	T7667	det	a,density
R4896	T7667	T7665	pobj	density,at
R4897	T7668	T7667	prep	of,density
R4898	T7669	T7668	pobj	20000,of
R4899	T7670	T7671	punct	/,well
R4900	T7671	T7669	prep	well,20000
R4901	T7672	T7648	cc	and,plated
R4902	T7673	T7648	conj	incubated,plated
R4903	T7674	T7673	prep	at,incubated
R4904	T7675	T7676	nummod	33,°C
R4905	T7676	T7674	pobj	°C,at
R4906	T7677	T7673	advmod	overnight,incubated
R4907	T7678	T7648	punct	.,plated
R4908	T7680	T7681	det	The,day
R4909	T7681	T7683	npadvmod	day,added
R4910	T7682	T7681	amod	following,day
R4911	T7684	T7683	punct	", ",added
R4912	T7685	T7686	compound	CellTiter,GLO®
R4913	T7686	T7688	compound	GLO®,Reagent
R4914	T7687	T7686	punct	-,GLO®
R4915	T7688	T7683	nsubjpass	Reagent,added
R4916	T7689	T7683	auxpass	was,added
R4917	T7690	T7683	prep	to,added
R4918	T7691	T7692	det	each,well
R4919	T7692	T7690	pobj	well,to
R4920	T7693	T7683	cc	and,added
R4921	T7694	T7695	compound	cell,lysis
R4922	T7695	T7696	nsubjpass	lysis,induced
R4923	T7696	T7683	conj	induced,added
R4924	T7697	T7696	auxpass	was,induced
R4925	T7698	T7696	prep	by,induced
R4926	T7699	T7698	pcomp	mixing,by
R4927	T7700	T7701	nummod	2,minutes
R4928	T7701	T7699	npadvmod	minutes,mixing
R4929	T7702	T7696	punct	.,induced
R4930	T7704	T7705	det	An,curve
R4931	T7705	T7708	nsubjpass	curve,prepared
R4932	T7706	T7705	nmod	ATP,curve
R4933	T7707	T7705	amod	standard,curve
R4934	T7709	T7708	auxpass	was,prepared
R4935	T7710	T7708	prep	in,prepared
R4936	T7711	T7712	det	the,plate
R4937	T7712	T7710	pobj	plate,in
R4938	T7713	T7712	amod	same,plate
R4939	T7714	T7708	punct	.,prepared
R4940	T7716	T7717	prep	Before,adapted
R4941	T7718	T7716	pcomp	recording,Before
R4942	T7719	T7718	dobj	luminescence,recording
R4943	T7720	T7718	prep	with,recording
R4944	T7721	T7722	det	a,luminometer
R4945	T7722	T7720	pobj	luminometer,with
R4946	T7723	T7722	compound	microplate,luminometer
R4947	T7724	T7722	punct	(,luminometer
R4948	T7725	T7726	compound	MicroLumat,96V
R4949	T7726	T7722	appos	96V,luminometer
R4950	T7727	T7726	compound	Plus,96V
R4951	T7728	T7726	compound	LB,96V
R4952	T7729	T7730	punct	", ",Techonologies
R4953	T7730	T7722	parataxis	Techonologies,luminometer
R4954	T7731	T7730	compound	Berthold,Techonologies
R4955	T7732	T7730	punct	),Techonologies
R4956	T7733	T7717	punct	", ",adapted
R4957	T7734	T7735	det	the,plate
R4958	T7735	T7717	nsubjpass	plate,adapted
R4959	T7736	T7717	auxpass	was,adapted
R4960	T7737	T7717	dep	dark,adapted
R4961	T7738	T7717	prep	for,adapted
R4962	T7739	T7740	nummod	10,minutes
R4963	T7740	T7738	pobj	minutes,for
R4964	T7741	T7717	prep	at,adapted
R4965	T7742	T7743	compound	room,temperature
R4966	T7743	T7741	pobj	temperature,at
R4967	T7744	T7745	aux	to,stabilize
R4968	T7745	T7717	advcl	stabilize,adapted
R4969	T7746	T7747	det	the,signal
R4970	T7747	T7745	dobj	signal,stabilize
R4971	T7748	T7747	compound	luminescence,signal
R4972	T7749	T7717	punct	.,adapted
R4973	T7780	T7781	compound	Hydrogen,peroxide
R4974	T7781	T7782	compound	peroxide,treatment
R4975	T7782	T7783	compound	treatment,assay
R4976	T7785	T7786	nsubjpass	Cells,plated
R4977	T7787	T7786	auxpass	were,plated
R4978	T7788	T7786	prep	at,plated
R4979	T7789	T7790	det	a,density
R4980	T7790	T7788	pobj	density,at
R4981	T7791	T7790	prep	of,density
R4982	T7792	T7793	nummod	"10,000",cells
R4983	T7793	T7791	pobj	cells,of
R4984	T7794	T7795	punct	/,well
R4985	T7795	T7793	prep	well,cells
R4986	T7796	T7786	prep	in,plated
R4987	T7797	T7798	nummod	96,well
R4988	T7798	T7800	compound	well,plates
R4989	T7799	T7798	punct	-,well
R4990	T7800	T7796	pobj	plates,in
R4991	T7801	T7786	cc	and,plated
R4992	T7802	T7786	conj	incubated,plated
R4993	T7803	T7802	prep	at,incubated
R4994	T7804	T7805	nummod	33,°C
R4995	T7805	T7803	pobj	°C,at
R4996	T7806	T7802	advmod	overnight,incubated
R4997	T7807	T7786	punct	.,plated
R4998	T7809	T7810	det	The,day
R4999	T7810	T7812	npadvmod	day,dispersed
R5000	T7811	T7810	amod	following,day
R5001	T7813	T7812	punct	", ",dispersed
R5002	T7814	T7815	amod	fresh,media
R5003	T7815	T7812	nsubjpass	media,dispersed
R5004	T7816	T7815	acl	containing,media
R5005	T7817	T7818	amod	varying,concentrations
R5006	T7818	T7816	dobj	concentrations,containing
R5007	T7819	T7818	prep	of,concentrations
R5008	T7820	T7821	compound	hydrogen,peroxide
R5009	T7821	T7819	pobj	peroxide,of
R5010	T7822	T7812	auxpass	was,dispersed
R5011	T7823	T7812	prep	to,dispersed
R5012	T7824	T7825	det	each,well
R5013	T7825	T7823	pobj	well,to
R5014	T7826	T7812	punct	.,dispersed
R5015	T7828	T7829	nsubjpass	Cells,incubated
R5016	T7830	T7829	auxpass	were,incubated
R5017	T7831	T7829	prep	in,incubated
R5018	T7832	T7833	det	the,presence
R5019	T7833	T7831	pobj	presence,in
R5020	T7834	T7833	prep	of,presence
R5021	T7835	T7836	compound	hydrogen,peroxide
R5022	T7836	T7834	pobj	peroxide,of
R5023	T7837	T7829	prep	for,incubated
R5024	T7838	T7839	nummod	24,hours
R5025	T7839	T7837	pobj	hours,for
R5026	T7840	T7829	punct	", ",incubated
R5027	T7841	T7829	prep	at,incubated
R5028	T7842	T7843	nummod	33,°C
R5029	T7843	T7841	pobj	°C,at
R5030	T7844	T7829	punct	", ",incubated
R5031	T7845	T7829	cc	and,incubated
R5032	T7846	T7847	nsubjpass	viability,measured
R5033	T7847	T7829	conj	measured,incubated
R5034	T7848	T7847	auxpass	was,measured
R5035	T7849	T7847	advcl	using,measured
R5036	T7850	T7851	det	the,Assay
R5037	T7851	T7849	dobj	Assay,using
R5038	T7852	T7853	nmod	CellTiter,96®
R5039	T7853	T7851	nmod	96®,Assay
R5040	T7854	T7853	punct	-,96®
R5041	T7855	T7851	amod	AQueous,Assay
R5042	T7856	T7851	amod	Non-Radioactive,Assay
R5043	T7857	T7851	compound	Cell,Assay
R5044	T7858	T7851	compound	Proliferation,Assay
R5045	T7859	T7860	punct	(,Promega
R5046	T7860	T7851	parataxis	Promega,Assay
R5047	T7861	T7860	punct	),Promega
R5048	T7862	T7849	punct	", ",using
R5049	T7863	T7849	prep	according,using
R5050	T7864	T7863	prep	to,according
R5051	T7865	T7866	det	the,manufacturer
R5052	T7866	T7867	poss	manufacturer,specifications
R5053	T7867	T7864	pobj	specifications,to
R5054	T7868	T7866	case	's,manufacturer
R5055	T7869	T7847	punct	.,measured
R3000	T5236	T5237	nmod	Antibody,reagents
R3001	T5238	T5236	cc	and,Antibody
R3002	T5239	T5240	compound	cell,staining
R3003	T5240	T5236	conj	staining,Antibody
R3004	T5242	T5243	nmod	Nestin,antibodies
R3005	T5243	T5282	nsubjpass	antibodies,obtained
R3006	T5244	T5245	punct	(,μg
R3007	T5245	T5242	parataxis	μg,Nestin
R3008	T5246	T5247	compound	clone,Rat
R3009	T5247	T5245	dep	Rat,μg
R3010	T5248	T5247	nummod	401,Rat
R3011	T5249	T5245	punct	", ",μg
R3012	T5250	T5245	nummod	2,μg
R3013	T5251	T5252	punct	/,ml
R3014	T5252	T5245	prep	ml,μg
R3015	T5253	T5245	punct	),μg
R3016	T5254	T5242	punct	", ",Nestin
R3017	T5255	T5242	conj	Lamp,Nestin
R3018	T5256	T5255	nummod	1,Lamp
R3019	T5257	T5258	punct	(,μg
R3020	T5258	T5255	parataxis	μg,Lamp
R3021	T5259	T5260	compound	clone,1D4B
R3022	T5260	T5258	dep	1D4B,μg
R3023	T5261	T5258	punct	", ",μg
R3024	T5262	T5258	nummod	6,μg
R3025	T5263	T5264	punct	/,ml
R3026	T5264	T5258	prep	ml,μg
R3027	T5265	T5258	punct	),μg
R3028	T5266	T5255	punct	", ",Lamp
R3029	T5267	T5255	cc	and,Lamp
R3030	T5268	T5255	conj	Lamp,Lamp
R3031	T5269	T5268	nummod	2,Lamp
R3032	T5270	T5271	punct	(,μg
R3033	T5271	T5268	parataxis	μg,Lamp
R3034	T5272	T5273	compound	clone,Abl
R3035	T5273	T5271	dep	Abl,μg
R3036	T5274	T5273	punct	-,Abl
R3037	T5275	T5273	nummod	93,Abl
R3038	T5276	T5271	punct	;,μg
R3039	T5277	T5271	nummod	6,μg
R3040	T5278	T5279	punct	/,ml
R3041	T5279	T5271	prep	ml,μg
R3042	T5280	T5271	punct	),μg
R3043	T5281	T5243	amod	monoclonal,antibodies
R3044	T5283	T5282	auxpass	were,obtained
R3045	T5284	T5282	prep	from,obtained
R3046	T5285	T5286	det	the,Bank
R3047	T5286	T5284	pobj	Bank,from
R3048	T5287	T5286	compound	Developmental,Bank
R3049	T5288	T5286	compound	Studies,Bank
R3050	T5289	T5286	compound	Hybridoma,Bank
R3051	T5290	T5286	punct	", ",Bank
R3052	T5291	T5286	acl	maintained,Bank
R3053	T5292	T5291	agent	by,maintained
R3054	T5293	T5294	det	The,University
R3055	T5294	T5295	nmod	University,Department
R3056	T5295	T5292	pobj	Department,by
R3057	T5296	T5294	prep	of,University
R3058	T5297	T5296	pobj	Iowa,of
R3059	T5298	T5295	punct	", ",Department
R3060	T5299	T5295	prep	of,Department
R3061	T5300	T5301	compound	Biological,Sciences
R3062	T5301	T5299	pobj	Sciences,of
R3063	T5302	T5282	punct	.,obtained
R3064	T5304	T5305	nsubjpass	Batp1,described
R3065	T5306	T5307	punct	(,μg
R3066	T5307	T5304	parataxis	μg,Batp1
R3067	T5308	T5307	nummod	1,μg
R3068	T5309	T5310	punct	/,ml
R3069	T5310	T5307	prep	ml,μg
R3070	T5311	T5307	punct	),μg
R3071	T5312	T5305	auxpass	was,described
R3072	T5313	T5305	advmod	previously,described
R3073	T5314	T5315	punct	[,12
R3074	T5315	T5305	parataxis	12,described
R3075	T5316	T5315	punct	],12
R3076	T5317	T5305	punct	.,described
R3077	T5319	T5320	amod	Anti-subunit,c
R3078	T5320	T5321	amod	c,antibody
R3079	T5321	T5322	nsubjpass	antibody,provided
R3080	T5323	T5324	punct	(,μg
R3081	T5324	T5321	parataxis	μg,antibody
R3082	T5325	T5324	nummod	0.7,μg
R3083	T5326	T5327	punct	/,ml
R3084	T5327	T5324	prep	ml,μg
R3085	T5328	T5324	punct	),μg
R3086	T5329	T5322	auxpass	was,provided
R3087	T5330	T5322	advmod	kindly,provided
R3088	T5331	T5322	agent	by,provided
R3089	T5332	T5333	compound	Dr.,Kominami
R3090	T5333	T5331	pobj	Kominami,by
R3091	T5334	T5335	punct	(,University
R3092	T5335	T5333	parataxis	University,Kominami
R3093	T5336	T5335	compound	Juntendo,University
R3094	T5337	T5335	punct	", ",University
R3095	T5338	T5335	npadvmod	Tokyo,University
R3096	T5339	T5335	punct	", ",University
R3097	T5340	T5335	npadvmod	Japan,University
R3098	T5341	T5335	punct	),University
R3099	T5342	T5322	punct	.,provided
R3100	T5344	T5345	amod	Additional,antibodies
R3101	T5345	T5346	nsubj	antibodies,were
R3102	T5347	T5348	mark	as,follows
R3103	T5348	T5346	advcl	follows,were
R3104	T5349	T5346	punct	: ,were
R3105	T5350	T5346	dep	GFAP,were
R3106	T5351	T5350	punct	", ",GFAP
R3107	T5352	T5350	npadvmod	1,GFAP
R3108	T5353	T5354	punct	:,2000
R3109	T5354	T5352	prep	2000,1
R3110	T5355	T5356	punct	(,Corporation
R3111	T5356	T5350	parataxis	Corporation,GFAP
R3112	T5357	T5356	compound	DAKO,Corporation
R3113	T5358	T5356	punct	),Corporation
R3114	T5359	T5350	punct	;,GFAP
R3115	T5360	T5350	appos	calbindin,GFAP
R3116	T5361	T5360	punct	", ",calbindin
R3117	T5362	T5360	npadvmod	1,calbindin
R3118	T5363	T5364	punct	:,5000
R3119	T5364	T5362	prep	5000,1
R3120	T5365	T5366	punct	(,Sigma
R3121	T5366	T5360	parataxis	Sigma,calbindin
R3122	T5367	T5366	punct	),Sigma
R3123	T5368	T5350	punct	;,GFAP
R3124	T5369	T5350	appos	NeuN,GFAP
R3125	T5370	T5369	punct	", ",NeuN
R3126	T5371	T5372	nummod	10,μg
R3127	T5372	T5369	npadvmod	μg,NeuN
R3128	T5373	T5374	punct	/,ml
R3129	T5374	T5372	prep	ml,μg
R3130	T5375	T5376	punct	(,Chemicon
R3131	T5376	T5369	parataxis	Chemicon,NeuN
R3132	T5377	T5376	punct	),Chemicon
R3133	T5378	T5350	punct	;,GFAP
R3134	T5379	T5380	compound	SV40,antigen
R3135	T5380	T5350	appos	antigen,GFAP
R3136	T5381	T5380	compound	T,antigen
R3137	T5382	T5380	punct	(,antigen
R3138	T5383	T5380	appos	Pab,antigen
R3139	T5384	T5383	nummod	101,Pab
R3140	T5385	T5380	punct	),antigen
R3141	T5386	T5380	punct	", ",antigen
R3142	T5387	T5388	nummod	2,μg
R3143	T5388	T5380	npadvmod	μg,antigen
R3144	T5389	T5390	punct	/,ml
R3145	T5390	T5388	prep	ml,μg
R3146	T5391	T5392	punct	(,Biotechnology
R3147	T5392	T5380	parataxis	Biotechnology,antigen
R3148	T5393	T5392	compound	Santa,Biotechnology
R3149	T5394	T5392	compound	Cruz,Biotechnology
R3150	T5395	T5392	punct	),Biotechnology
R3151	T5396	T5350	punct	;,GFAP
R3152	T5397	T5398	compound	cathepsin,D
R3153	T5398	T5350	appos	D,GFAP
R3154	T5399	T5398	punct	(,D
R3155	T5400	T5398	appos	C,D
R3156	T5401	T5400	punct	-,C
R3157	T5402	T5400	nummod	20,C
R3158	T5403	T5398	punct	),D
R3159	T5404	T5398	punct	", ",D
R3160	T5405	T5406	nummod	2,μg
R3161	T5406	T5398	npadvmod	μg,D
R3162	T5407	T5408	punct	/,ml
R3163	T5408	T5406	prep	ml,μg
R3164	T5409	T5410	punct	(,Biotechnology
R3165	T5410	T5398	parataxis	Biotechnology,D
R3166	T5411	T5410	compound	Santa,Biotechnology
R3167	T5412	T5410	compound	Cruz,Biotechnology
R3168	T5413	T5410	punct	),Biotechnology
R3169	T5414	T5350	punct	;,GFAP
R3170	T5415	T5416	compound	cytochrome,subunit
R3171	T5416	T5350	appos	subunit,GFAP
R3172	T5417	T5416	compound	c,subunit
R3173	T5418	T5416	compound	oxidase,subunit
R3174	T5419	T5416	nummod	IV,subunit
R3175	T5420	T5416	punct	(,subunit
R3176	T5421	T5416	appos	cox4,subunit
R3177	T5422	T5416	punct	),subunit
R3178	T5423	T5416	punct	", ",subunit
R3179	T5424	T5416	npadvmod	1,subunit
R3180	T5425	T5426	punct	:,1000
R3181	T5426	T5424	prep	1000,1
R3182	T5427	T5428	punct	(,Clontech
R3183	T5428	T5416	parataxis	Clontech,subunit
R3184	T5429	T5428	compound	BD,Clontech
R3185	T5430	T5428	compound	Biosciences,Clontech
R3186	T5431	T5428	punct	),Clontech
R3187	T5432	T5350	punct	;,GFAP
R3188	T5433	T5350	appos	PDI,GFAP
R3189	T5434	T5433	punct	(,PDI
R3190	T5435	T5433	appos	H,PDI
R3191	T5436	T5435	punct	-,H
R3192	T5437	T5435	nummod	160,H
R3193	T5438	T5433	punct	),PDI
R3194	T5439	T5433	punct	", ",PDI
R3195	T5440	T5441	nummod	2,μg
R3196	T5441	T5433	npadvmod	μg,PDI
R3197	T5442	T5443	punct	/,ml
R3198	T5443	T5441	prep	ml,μg
R3199	T5444	T5445	punct	(,Biotechnology
R3200	T5445	T5433	parataxis	Biotechnology,PDI
R3201	T5446	T5445	compound	Santa,Biotechnology
R3202	T5447	T5445	compound	Cruz,Biotechnology
R3203	T5448	T5445	punct	),Biotechnology
R3204	T5449	T5350	punct	;,GFAP
R3205	T5450	T5350	appos	GM130,GFAP
R3206	T5451	T5450	punct	", ",GM130
R3207	T5452	T5453	nummod	1,μg
R3208	T5453	T5450	npadvmod	μg,GM130
R3209	T5454	T5455	punct	/,ml
R3210	T5455	T5453	prep	ml,μg
R3211	T5456	T5457	punct	(,Labs
R3212	T5457	T5450	parataxis	Labs,GM130
R3213	T5458	T5457	compound	BD,Labs
R3214	T5459	T5457	compound	Transduction,Labs
R3215	T5460	T5457	punct	),Labs
R3216	T5461	T5350	punct	;,GFAP
R3217	T5462	T5463	compound	α,tubulin
R3218	T5463	T5350	appos	tubulin,GFAP
R3219	T5464	T5463	punct	-,tubulin
R3220	T5465	T5463	punct	", ",tubulin
R3221	T5466	T5463	npadvmod	1,tubulin
R3222	T5467	T5468	punct	:,"15,000"
R3223	T5468	T5466	prep	"15,000",1
R3224	T5469	T5470	punct	(,Sigma
R3225	T5470	T5463	parataxis	Sigma,tubulin
R3226	T5471	T5470	punct	),Sigma
R3227	T5472	T5350	punct	;,GFAP
R3228	T5473	T5350	appos	grp75,GFAP
R3229	T5474	T5473	punct	", ",grp75
R3230	T5475	T5473	npadvmod	1,grp75
R3231	T5476	T5477	punct	:,200
R3232	T5477	T5475	prep	200,1
R3233	T5478	T5479	punct	(,Stressgen
R3234	T5479	T5473	parataxis	Stressgen,grp75
R3235	T5480	T5479	punct	),Stressgen
R3236	T5481	T5350	punct	;,GFAP
R3237	T5482	T5483	amod	early,antigen
R3238	T5483	T5350	appos	antigen,GFAP
R3239	T5484	T5483	compound	endosome,antigen
R3240	T5485	T5483	punct	-,antigen
R3241	T5486	T5483	nummod	1,antigen
R3242	T5487	T5483	punct	(,antigen
R3243	T5488	T5483	appos	EEA1,antigen
R3244	T5489	T5483	punct	),antigen
R3245	T5490	T5483	punct	", ",antigen
R3246	T5491	T5492	nummod	2,μg
R3247	T5492	T5483	npadvmod	μg,antigen
R3248	T5493	T5494	punct	/,ml
R3249	T5494	T5492	prep	ml,μg
R3250	T5495	T5496	punct	(,Biotechnology
R3251	T5496	T5483	parataxis	Biotechnology,antigen
R3252	T5497	T5496	compound	Santa,Biotechnology
R3253	T5498	T5496	compound	Cruz,Biotechnology
R3254	T5499	T5496	punct	),Biotechnology
R3255	T5500	T5350	punct	;,GFAP
R3256	T5501	T5350	appos	rab,GFAP
R3257	T5502	T5501	nummod	7,rab
R3258	T5503	T5501	punct	", ",rab
R3259	T5504	T5505	nummod	4,μg
R3260	T5505	T5501	npadvmod	μg,rab
R3261	T5506	T5507	punct	/,ml
R3262	T5507	T5505	prep	ml,μg
R3263	T5508	T5509	punct	(,Biotechnology
R3264	T5509	T5501	parataxis	Biotechnology,rab
R3265	T5510	T5509	compound	Santa,Biotechnology
R3266	T5511	T5509	compound	Cruz,Biotechnology
R3267	T5512	T5509	punct	),Biotechnology
R3268	T5513	T5346	punct	.,were
R3269	T5515	T5516	det	All,antibodies
R3270	T5516	T5519	nsubjpass	antibodies,obtained
R3271	T5517	T5516	amod	fluorescent,antibodies
R3272	T5518	T5516	amod	secondary,antibodies
R3273	T5520	T5519	auxpass	were,obtained
R3274	T5521	T5519	prep	from,obtained
R3275	T5522	T5523	compound	Jackson,Laboratories
R3276	T5523	T5521	pobj	Laboratories,from
R3277	T5524	T5523	compound	ImmunoResearch,Laboratories
R3278	T5525	T5519	cc	and,obtained
R3279	T5526	T5527	npadvmod	HRP,conjugated
R3280	T5527	T5529	amod	conjugated,antibodies
R3281	T5528	T5527	punct	-,conjugated
R3282	T5529	T5531	nsubjpass	antibodies,obtained
R3283	T5530	T5529	amod	secondary,antibodies
R3284	T5531	T5519	conj	obtained,obtained
R3285	T5532	T5531	auxpass	were,obtained
R3286	T5533	T5531	prep	from,obtained
R3287	T5534	T5535	compound	Amersham,Biosciences
R3288	T5535	T5533	pobj	Biosciences,from
R3289	T5536	T5531	punct	.,obtained
R3290	T5538	T5539	amod	Additional,markers
R3291	T5539	T5541	nsubj	markers,were
R3292	T5540	T5539	compound	cell,markers
R3293	T5542	T5543	mark	as,follows
R3294	T5543	T5541	advcl	follows,were
R3295	T5544	T5541	punct	: ,were
R3296	T5545	T5546	compound	VVL,biotin
R3297	T5546	T5541	dep	biotin,were
R3298	T5547	T5546	punct	-,biotin
R3299	T5548	T5546	punct	", ",biotin
R3300	T5549	T5546	npadvmod	1,biotin
R3301	T5550	T5551	punct	:,2000
R3302	T5551	T5549	prep	2000,1
R3303	T5552	T5553	punct	(,Laboratories
R3304	T5553	T5546	parataxis	Laboratories,biotin
R3305	T5554	T5553	compound	Vector,Laboratories
R3306	T5555	T5553	punct	),Laboratories
R3307	T5556	T5546	punct	", ",biotin
R3308	T5557	T5558	nummod	"10,000",MW
R3309	T5558	T5559	compound	MW,FITC
R3310	T5559	T5546	conj	FITC,biotin
R3311	T5560	T5559	compound	dextran,FITC
R3312	T5561	T5559	punct	-,FITC
R3313	T5562	T5559	punct	", ",FITC
R3314	T5563	T5564	nummod	1,mg
R3315	T5564	T5559	npadvmod	mg,FITC
R3316	T5565	T5566	punct	/,ml
R3317	T5566	T5564	prep	ml,mg
R3318	T5567	T5559	cc	and,FITC
R3319	T5568	T5569	compound	Lysotracker,DND
R3320	T5569	T5559	conj	DND,FITC
R3321	T5570	T5569	compound	Red,DND
R3322	T5571	T5569	punct	-,DND
R3323	T5572	T5569	nummod	99,DND
R3324	T5573	T5569	punct	", ",DND
R3325	T5574	T5575	nummod	500,nM
R3326	T5575	T5569	npadvmod	nM,DND
R3327	T5576	T5577	punct	(,Probes
R3328	T5577	T5569	parataxis	Probes,DND
R3329	T5578	T5577	compound	Molecular,Probes
R3330	T5579	T5577	punct	),Probes
R3331	T5580	T5541	punct	.,were
R3332	T5714	T5713	punct	", ",Generation
R3333	T5715	T5713	conj	maintenance,Generation
R3334	T5716	T5715	cc	and,maintenance
R3335	T5717	T5715	conj	differentiation,maintenance
R3336	T5718	T5713	prep	of,Generation
R3337	T5719	T5720	nmod	CbCln3,lines
R3338	T5720	T5718	pobj	lines,of
R3339	T5721	T5720	amod	cerebellar,lines
R3340	T5722	T5723	amod	neuronal,precursor
R3341	T5723	T5720	compound	precursor,lines
R3342	T5725	T5726	nmod	Cln3Δex7,mice
R3343	T5726	T5732	nsubjpass	mice,described
R3344	T5727	T5725	punct	/,Cln3Δex7
R3345	T5728	T5725	nummod	8,Cln3Δex7
R3346	T5729	T5726	amod	knock,mice
R3347	T5730	T5729	punct	-,knock
R3348	T5731	T5729	prt	in,knock
R3349	T5733	T5732	aux	have,described
R3350	T5734	T5732	auxpass	been,described
R3351	T5735	T5732	advmod	previously,described
R3352	T5736	T5737	punct	[,12
R3353	T5737	T5732	parataxis	12,described
R3354	T5738	T5737	punct	],12
R3355	T5739	T5732	punct	.,described
R3356	T5741	T5742	compound	Littermate,pups
R3357	T5742	T5743	nsubjpass	pups,used
R3358	T5744	T5742	prep	from,pups
R3359	T5745	T5746	compound	heterozygote,crosses
R3360	T5746	T5744	pobj	crosses,from
R3361	T5747	T5743	auxpass	were,used
R3362	T5748	T5743	prep	for,used
R3363	T5749	T5750	amod	primary,establishment
R3364	T5750	T5748	pobj	establishment,for
R3365	T5751	T5750	compound	culture,establishment
R3366	T5752	T5743	punct	", ",used
R3367	T5753	T5743	prep	by,used
R3368	T5754	T5755	advmod	previously,described
R3369	T5755	T5756	amod	described,methods
R3370	T5756	T5753	pobj	methods,by
R3371	T5757	T5758	dep	that,yield
R3372	T5758	T5756	relcl	yield,methods
R3373	T5759	T5760	amod	cerebellar,neuron
R3374	T5760	T5762	npadvmod	neuron,enriched
R3375	T5761	T5760	compound	granule,neuron
R3376	T5762	T5764	amod	enriched,cultures
R3377	T5763	T5762	punct	-,enriched
R3378	T5764	T5758	dobj	cultures,yield
R3379	T5765	T5766	punct	[,26
R3380	T5766	T5758	parataxis	26,yield
R3381	T5767	T5766	punct	],26
R3382	T5768	T5743	punct	.,used
R3383	T5770	T5771	amod	Postnatal,day
R3384	T5771	T5772	nmod	day,cerebella
R3385	T5772	T5777	nsubjpass	cerebella,dissected
R3386	T5773	T5771	nummod	4,day
R3387	T5774	T5771	punct	(,day
R3388	T5775	T5771	appos	P4,day
R3389	T5776	T5772	punct	),cerebella
R3390	T5778	T5777	auxpass	were,dissected
R3391	T5779	T5777	prep	in,dissected
R3392	T5780	T5781	poss	Hank,Solution
R3393	T5781	T5779	pobj	Solution,in
R3394	T5782	T5780	case	's,Hank
R3395	T5783	T5781	compound	Balanced,Solution
R3396	T5784	T5781	compound	Salt,Solution
R3397	T5785	T5781	punct	(,Solution
R3398	T5786	T5781	appos	HBSS,Solution
R3399	T5787	T5788	punct	", ",Sigma
R3400	T5788	T5781	parataxis	Sigma,Solution
R3401	T5789	T5788	punct	),Sigma
R3402	T5790	T5781	punct	", ",Solution
R3403	T5791	T5781	acl	supplemented,Solution
R3404	T5792	T5791	prep	with,supplemented
R3405	T5793	T5794	nummod	35,mM
R3406	T5794	T5795	compound	mM,glucose
R3407	T5795	T5792	pobj	glucose,with
R3408	T5796	T5777	punct	.,dissected
R3409	T5798	T5799	compound	Tail,biopsies
R3410	T5799	T5800	nsubjpass	biopsies,collected
R3411	T5801	T5800	auxpass	were,collected
R3412	T5802	T5800	advmod	also,collected
R3413	T5803	T5800	prep	for,collected
R3414	T5804	T5805	amod	genomic,isolation
R3415	T5805	T5803	pobj	isolation,for
R3416	T5806	T5805	compound	DNA,isolation
R3417	T5807	T5805	cc	and,isolation
R3418	T5808	T5809	amod	genotypic,analysis
R3419	T5809	T5805	conj	analysis,isolation
R3420	T5810	T5800	punct	.,collected
R3421	T5812	T5813	compound	Trypsin,EDTA
R3422	T5813	T5815	nsubj	EDTA,helped
R3423	T5814	T5813	punct	/,EDTA
R3424	T5816	T5817	punct	(,Sigma
R3425	T5817	T5813	parataxis	Sigma,EDTA
R3426	T5818	T5819	nummod	10,mg
R3427	T5819	T5817	dep	mg,Sigma
R3428	T5820	T5821	punct	/,ml
R3429	T5821	T5819	prep	ml,mg
R3430	T5822	T5817	punct	", ",Sigma
R3431	T5823	T5817	punct	),Sigma
R3432	T5824	T5813	cc	and,EDTA
R3433	T5825	T5813	conj	DNase,EDTA
R3434	T5826	T5825	nummod	I,DNase
R3435	T5827	T5828	punct	(,Sigma
R3436	T5828	T5825	parataxis	Sigma,DNase
R3437	T5829	T5830	nummod	100,μg
R3438	T5830	T5828	dep	μg,Sigma
R3439	T5831	T5832	punct	/,ml
R3440	T5832	T5830	prep	ml,μg
R3441	T5833	T5828	punct	", ",Sigma
R3442	T5834	T5828	punct	),Sigma
R3443	T5835	T5813	punct	", ",EDTA
R3444	T5836	T5813	acl	suspended,EDTA
R3445	T5837	T5836	prep	in,suspended
R3446	T5838	T5837	pobj	HBSS,in
R3447	T5839	T5815	punct	", ",helped
R3448	T5840	T5815	xcomp	dissociate,helped
R3449	T5841	T5840	dobj	cerebella,dissociate
R3450	T5842	T5840	prep	for,dissociate
R3451	T5843	T5844	amod	primary,culture
R3452	T5844	T5845	compound	culture,plating
R3453	T5845	T5842	pobj	plating,for
R3454	T5846	T5845	prep	onto,plating
R3455	T5847	T5848	nummod	0.01,%
R3456	T5848	T5849	compound	%,poly-ornithine
R3457	T5849	T5850	npadvmod	poly-ornithine,coated
R3458	T5850	T5854	amod	coated,dishes
R3459	T5851	T5852	punct	(,Sigma
R3460	T5852	T5849	parataxis	Sigma,poly-ornithine
R3461	T5853	T5852	punct	),Sigma
R3462	T5854	T5846	pobj	dishes,onto
R3463	T5855	T5856	nummod	100,mm
R3464	T5856	T5854	compound	mm,dishes
R3465	T5857	T5815	punct	.,helped
R3466	T5859	T5860	amod	Primary,cultures
R3467	T5860	T5861	nsubjpass	cultures,cultured
R3468	T5862	T5860	prep	from,cultures
R3469	T5863	T5864	amod	individual,cerebella
R3470	T5864	T5862	pobj	cerebella,from
R3471	T5865	T5861	auxpass	were,cultured
R3472	T5866	T5861	advmod	overnight,cultured
R3473	T5867	T5861	prep	at,cultured
R3474	T5868	T5869	nummod	37,°C
R3475	T5869	T5867	pobj	°C,at
R3476	T5870	T5869	punct	", ",°C
R3477	T5871	T5872	nummod	5,%
R3478	T5872	T5873	compound	%,CO2
R3479	T5873	T5869	appos	CO2,°C
R3480	T5874	T5861	punct	", ",cultured
R3481	T5875	T5861	prep	in,cultured
R3482	T5876	T5877	compound	granule,neuron
R3483	T5877	T5878	compound	neuron,media
R3484	T5878	T5875	pobj	media,in
R3485	T5879	T5878	compound	growth,media
R3486	T5880	T5878	punct	(,media
R3487	T5881	T5882	poss	Dulbecco,Medium
R3488	T5882	T5878	appos	Medium,media
R3489	T5883	T5881	case	's,Dulbecco
R3490	T5884	T5882	compound	Modified,Medium
R3491	T5885	T5882	compound	Eagle,Medium
R3492	T5886	T5882	punct	[,Medium
R3493	T5887	T5882	appos	DMEM,Medium
R3494	T5888	T5882	punct	", ",Medium
R3495	T5889	T5890	compound	Gibco,#
R3496	T5890	T5882	appos	#,Medium
R3497	T5891	T5890	compound	BRL,#
R3498	T5892	T5890	nummod	11995,#
R3499	T5893	T5890	punct	-,#
R3500	T5894	T5890	nummod	065,#
R3501	T5895	T5882	punct	],Medium
R3502	T5896	T5882	punct	", ",Medium
R3503	T5897	T5898	nummod	10,%
R3504	T5898	T5882	appos	%,Medium
R3505	T5899	T5900	amod	fetal,serum
R3506	T5900	T5898	appos	serum,%
R3507	T5901	T5900	amod	bovine,serum
R3508	T5902	T5900	punct	[,serum
R3509	T5903	T5904	compound	Sigma,F
R3510	T5904	T5900	appos	F,serum
R3511	T5905	T5904	compound	#,F
R3512	T5906	T5904	punct	-,F
R3513	T5907	T5904	nummod	2442,F
R3514	T5908	T5882	punct	],Medium
R3515	T5909	T5882	punct	", ",Medium
R3516	T5910	T5882	acl	supplemented,Medium
R3517	T5911	T5910	prep	with,supplemented
R3518	T5912	T5913	nummod	24,mM
R3519	T5913	T5914	compound	mM,KCl
R3520	T5914	T5911	pobj	KCl,with
R3521	T5915	T5861	punct	),cultured
R3522	T5916	T5861	punct	.,cultured
R3523	T5918	T5919	nsubjpass	Infection,performed
R3524	T5920	T5919	auxpass	was,performed
R3525	T5921	T5922	det	the,day
R3526	T5922	T5919	npadvmod	day,performed
R3527	T5923	T5922	amod	following,day
R3528	T5924	T5919	prep	with,performed
R3529	T5925	T5926	amod	defective,retrovirus
R3530	T5926	T5927	nsubj	retrovirus,transducing
R3531	T5927	T5924	pcomp	transducing,with
R3532	T5928	T5929	det	the,antigen
R3533	T5929	T5927	dobj	antigen,transducing
R3534	T5930	T5931	npadvmod	temperature,sensitive
R3535	T5931	T5929	amod	sensitive,antigen
R3536	T5932	T5931	punct	-,sensitive
R3537	T5933	T5934	nmod	tsA58,U19
R3538	T5934	T5929	nmod	U19,antigen
R3539	T5935	T5934	punct	/,U19
R3540	T5936	T5929	amod	large,antigen
R3541	T5937	T5929	compound	T,antigen
R3542	T5938	T5929	cc	and,antigen
R3543	T5939	T5940	det	a,cassette
R3544	T5940	T5929	conj	cassette,antigen
R3545	T5941	T5940	compound	selection,cassette
R3546	T5942	T5943	compound	neomycin,resistance
R3547	T5943	T5940	compound	resistance,cassette
R3548	T5944	T5943	punct	-,resistance
R3549	T5945	T5946	punct	[,27
R3550	T5946	T5940	parataxis	27,cassette
R3551	T5947	T5946	punct	],27
R3552	T5948	T5927	punct	", ",transducing
R3553	T5949	T5950	mark	as,described
R3554	T5950	T5927	advcl	described,transducing
R3555	T5951	T5950	advmod	previously,described
R3556	T5952	T5953	punct	[,28
R3557	T5953	T5950	parataxis	28,described
R3558	T5954	T5953	punct	],28
R3559	T5955	T5919	punct	.,performed
R3560	T5957	T5958	prep	Following,shifted
R3561	T5959	T5957	pobj	infection,Following
R3562	T5960	T5958	punct	", ",shifted
R3563	T5961	T5958	nsubjpass	cultures,shifted
R3564	T5962	T5958	auxpass	were,shifted
R3565	T5963	T5958	prep	to,shifted
R3566	T5964	T5965	det	the,temperature
R3567	T5965	T5963	pobj	temperature,to
R3568	T5966	T5967	compound	tsA58,U19
R3569	T5967	T5969	npadvmod	U19,permissive
R3570	T5968	T5967	punct	/,U19
R3571	T5969	T5965	amod	permissive,temperature
R3572	T5970	T5965	compound	growth,temperature
R3573	T5971	T5965	prep	of,temperature
R3574	T5972	T5973	nummod	33,°C
R3575	T5973	T5971	pobj	°C,of
R3576	T5974	T5958	cc	and,shifted
R3577	T5975	T5976	nsubj	selection,was
R3578	T5976	T5958	conj	was,shifted
R3579	T5977	T5976	prep	in,was
R3580	T5978	T5979	det	the,media
R3581	T5979	T5977	pobj	media,in
R3582	T5980	T5979	amod	same,media
R3583	T5981	T5979	compound	growth,media
R3584	T5982	T5979	prep	as,media
R3585	T5983	T5982	pcomp	above,as
R3586	T5984	T5976	punct	", ",was
R3587	T5985	T5976	prep	with,was
R3588	T5986	T5987	nummod	200,μg
R3589	T5987	T5988	nmod	μg,G418
R3590	T5988	T5985	pobj	G418,with
R3591	T5989	T5990	punct	/,ml
R3592	T5990	T5987	prep	ml,μg
R3593	T5991	T5976	punct	.,was
R3594	T5993	T5994	advmod	Conditionally,immortalized
R3595	T5994	T5995	amod	immortalized,colonies
R3596	T5995	T5996	nsubjpass	colonies,isolated
R3597	T5997	T5996	auxpass	were,isolated
R3598	T5998	T5999	quantmod	3,9
R3599	T5999	T6001	nummod	9,weeks
R3600	T6000	T5999	punct	–,9
R3601	T6001	T6002	npadvmod	weeks,post-infection
R3602	T6002	T5996	advmod	post-infection,isolated
R3603	T6003	T6004	cc	and,expanded
R3604	T6004	T5996	dep	expanded,isolated
R3605	T6005	T6004	prep	for,expanded
R3606	T6006	T6007	amod	frozen,stocks
R3607	T6007	T6005	pobj	stocks,for
R3608	T6008	T6007	cc	and,stocks
R3609	T6009	T6010	amod	further,isolation
R3610	T6010	T6007	conj	isolation,stocks
R3611	T6011	T6010	compound	sub-clone,isolation
R3612	T6012	T5996	punct	.,isolated
R3613	T6014	T6015	amod	Multiple,lines
R3614	T6015	T6017	nsubjpass	lines,obtained
R3615	T6016	T6015	amod	clonal,lines
R3616	T6018	T6017	auxpass	were,obtained
R3617	T6019	T6017	prep	for,obtained
R3618	T6020	T6021	det	each,genotype
R3619	T6021	T6019	pobj	genotype,for
R3620	T6022	T6021	cc	and,genotype
R3621	T6023	T6024	det	all,phenotypes
R3622	T6024	T6021	conj	phenotypes,genotype
R3623	T6025	T6026	auxpass	were,confirmed
R3624	T6026	T6017	dep	confirmed,obtained
R3625	T6027	T6026	prep	in,confirmed
R3626	T6028	T6029	advmod	at,2
R3627	T6029	T6031	nummod	2,lines
R3628	T6030	T6029	advmod	least,2
R3629	T6031	T6027	pobj	lines,in
R3630	T6032	T6031	amod	independent,lines
R3631	T6033	T6031	compound	CbCln3,lines
R3632	T6034	T6031	compound	cell,lines
R3633	T6035	T6017	punct	.,obtained
R3634	T6037	T6038	compound	CbCln3,lines
R3635	T6038	T6040	nsubjpass	lines,maintained
R3636	T6039	T6038	compound	cell,lines
R3637	T6041	T6040	auxpass	were,maintained
R3638	T6042	T6040	prep	on,maintained
R3639	T6043	T6044	nummod	0.01,%
R3640	T6044	T6045	nmod	%,dishes
R3641	T6045	T6042	pobj	dishes,on
R3642	T6046	T6047	compound	poly,ornithine
R3643	T6047	T6049	npadvmod	ornithine,coated
R3644	T6048	T6047	punct	-,ornithine
R3645	T6049	T6045	amod	coated,dishes
R3646	T6050	T6040	prep	at,maintained
R3647	T6051	T6052	quantmod	30,90
R3648	T6052	T6054	nummod	90,%
R3649	T6053	T6052	punct	–,90
R3650	T6054	T6055	compound	%,confluency
R3651	T6055	T6050	pobj	confluency,at
R3652	T6056	T6040	punct	", ",maintained
R3653	T6057	T6040	prep	in,maintained
R3654	T6058	T6059	nummod	33,°C
R3655	T6059	T6057	pobj	°C,in
R3656	T6060	T6059	cc	and,°C
R3657	T6061	T6062	nummod	5,atmosphere
R3658	T6062	T6059	conj	atmosphere,°C
R3659	T6063	T6061	punct	%,5
R3660	T6064	T6062	compound	CO2,atmosphere
R3661	T6065	T6040	punct	.,maintained
R3662	T6067	T6068	compound	Passage,number
R3663	T6068	T6069	nsubjpass	number,recorded
R3664	T6070	T6069	auxpass	was,recorded
R3665	T6071	T6072	punct	(,passages
R3666	T6072	T6069	parataxis	passages,recorded
R3667	T6073	T6074	quantmod	up,20
R3668	T6074	T6072	nummod	20,passages
R3669	T6075	T6074	quantmod	to,20
R3670	T6076	T6074	punct	~,20
R3671	T6077	T6072	punct	),passages
R3672	T6078	T6069	punct	", ",recorded
R3673	T6079	T6069	cc	but,recorded
R3674	T6080	T6069	conj	had,recorded
R3675	T6081	T6082	det	no,impact
R3676	T6082	T6080	dobj	impact,had
R3677	T6083	T6082	amod	apparent,impact
R3678	T6084	T6082	prep	on,impact
R3679	T6085	T6084	pobj	phenotype,on
R3680	T6086	T6069	punct	.,recorded
R3681	T6088	T6089	amod	Neuronal,differentiation
R3682	T6089	T6090	nsubj	differentiation,was
R3683	T6091	T6092	mark	as,described
R3684	T6092	T6090	advcl	described,was
R3685	T6093	T6092	advmod	previously,described
R3686	T6094	T6095	punct	[,29
R3687	T6095	T6092	parataxis	29,described
R3688	T6096	T6095	punct	],29
R3689	T6097	T6090	prep	with,was
R3690	T6098	T6099	det	the,cocktail
R3691	T6099	T6097	pobj	cocktail,with
R3692	T6100	T6099	amod	following,cocktail
R3693	T6101	T6099	punct	: ,cocktail
R3694	T6102	T6103	nummod	10,ng
R3695	T6103	T6104	nmod	ng,FGF
R3696	T6104	T6099	appos	FGF,cocktail
R3697	T6105	T6106	punct	/,ml
R3698	T6106	T6103	prep	ml,ng
R3699	T6107	T6104	compound	α,FGF
R3700	T6108	T6104	punct	-,FGF
R3701	T6109	T6104	punct	", ",FGF
R3702	T6110	T6111	nummod	250,μM
R3703	T6111	T6112	compound	μM,IBMX
R3704	T6112	T6104	appos	IBMX,FGF
R3705	T6113	T6104	punct	", ",FGF
R3706	T6114	T6115	nummod	200,nM
R3707	T6115	T6116	compound	nM,TPA
R3708	T6116	T6104	appos	TPA,FGF
R3709	T6117	T6104	punct	", ",FGF
R3710	T6118	T6119	nummod	50,μM
R3711	T6119	T6120	compound	μM,forskolin
R3712	T6120	T6104	appos	forskolin,FGF
R3713	T6121	T6104	punct	", ",FGF
R3714	T6122	T6123	nummod	5,μM
R3715	T6123	T6124	compound	μM,dopamine
R3716	T6124	T6104	appos	dopamine,FGF
R3717	T6125	T6126	punct	(,Sigma
R3718	T6126	T6104	parataxis	Sigma,FGF
R3719	T6127	T6126	punct	),Sigma
R3720	T6128	T6090	punct	.,was
R3721	T6166	T6165	cc	and,Genotyping
R3722	T6167	T6168	compound	RT,PCR
R3723	T6168	T6165	conj	PCR,Genotyping
R3724	T6169	T6168	punct	-,PCR
R3725	T6171	T6172	amod	Genomic,DNA
R3726	T6172	T6173	nsubjpass	DNA,extracted
R3727	T6174	T6173	auxpass	was,extracted
R3728	T6175	T6173	prep	from,extracted
R3729	T6176	T6177	compound	tail,biopsies
R3730	T6177	T6175	pobj	biopsies,from
R3731	T6178	T6177	cc	and,biopsies
R3732	T6179	T6180	compound	cell,pellets
R3733	T6180	T6177	conj	pellets,biopsies
R3734	T6181	T6182	mark	as,described
R3735	T6182	T6173	advcl	described,extracted
R3736	T6183	T6184	punct	(,Cotman
R3737	T6184	T6173	meta	Cotman,extracted
R3738	T6185	T6184	nmod	et,Cotman
R3739	T6186	T6184	nmod	al.,Cotman
R3740	T6187	T6184	punct	", ",Cotman
R3741	T6188	T6184	nummod	2002,Cotman
R3742	T6189	T6184	punct	),Cotman
R3743	T6190	T6173	punct	.,extracted
R3744	T6192	T6193	nmod	Cln3Δex7,allele
R3745	T6193	T6199	compound	allele,genotyping
R3746	T6194	T6192	punct	/,Cln3Δex7
R3747	T6195	T6192	nummod	8,Cln3Δex7
R3748	T6196	T6193	amod	knock,allele
R3749	T6197	T6196	punct	-,knock
R3750	T6198	T6196	prt	in,knock
R3751	T6199	T6201	nsubj	genotyping,was
R3752	T6200	T6199	compound	PCR,genotyping
R3753	T6202	T6201	prep	with,was
R3754	T6203	T6204	amod	wild,type
R3755	T6204	T6206	compound	type,primers
R3756	T6205	T6204	punct	-,type
R3757	T6206	T6202	pobj	primers,with
R3758	T6207	T6206	punct	", ",primers
R3759	T6208	T6206	appos	WtF,primers
R3760	T6209	T6208	punct	(,WtF
R3761	T6210	T6211	nummod	5,CAGCATCTCCTCAGGGCTA
R3762	T6211	T6208	appos	CAGCATCTCCTCAGGGCTA,WtF
R3763	T6212	T6210	punct	',5
R3764	T6213	T6211	punct	-,CAGCATCTCCTCAGGGCTA
R3765	T6214	T6211	punct	-,CAGCATCTCCTCAGGGCTA
R3766	T6215	T6211	nummod	3,CAGCATCTCCTCAGGGCTA
R3767	T6216	T6211	punct	',CAGCATCTCCTCAGGGCTA
R3768	T6217	T6208	punct	),WtF
R3769	T6218	T6208	cc	and,WtF
R3770	T6219	T6208	conj	WtR,WtF
R3771	T6220	T6219	punct	(,WtR
R3772	T6221	T6222	nummod	5,CCAACATAGAAAGTAGGGTGTGC
R3773	T6222	T6219	appos	CCAACATAGAAAGTAGGGTGTGC,WtR
R3774	T6223	T6221	punct	',5
R3775	T6224	T6222	punct	-,CCAACATAGAAAGTAGGGTGTGC
R3776	T6225	T6222	punct	-,CCAACATAGAAAGTAGGGTGTGC
R3777	T6226	T6222	nummod	3,CCAACATAGAAAGTAGGGTGTGC
R3778	T6227	T6222	punct	',CCAACATAGAAAGTAGGGTGTGC
R3779	T6228	T6201	punct	),was
R3780	T6229	T6230	aux	to,yield
R3781	T6230	T6201	advcl	yield,was
R3782	T6231	T6232	det	a,band
R3783	T6232	T6230	dobj	band,yield
R3784	T6233	T6234	punct	~,250
R3785	T6234	T6235	nummod	250,bp
R3786	T6235	T6232	compound	bp,band
R3787	T6236	T6201	cc	and,was
R3788	T6237	T6238	amod	knock,primers
R3789	T6238	T6201	conj	primers,was
R3790	T6239	T6237	punct	-,knock
R3791	T6240	T6237	prt	in,knock
R3792	T6241	T6238	punct	", ",primers
R3793	T6242	T6238	appos	552F,primers
R3794	T6243	T6242	punct	(,552F
R3795	T6244	T6245	nummod	5,GAGCTTTGTTCTGGTTGCCTTC
R3796	T6245	T6242	appos	GAGCTTTGTTCTGGTTGCCTTC,552F
R3797	T6246	T6244	punct	',5
R3798	T6247	T6245	punct	-,GAGCTTTGTTCTGGTTGCCTTC
R3799	T6248	T6245	punct	-,GAGCTTTGTTCTGGTTGCCTTC
R3800	T6249	T6245	nummod	3,GAGCTTTGTTCTGGTTGCCTTC
R3801	T6250	T6245	punct	',GAGCTTTGTTCTGGTTGCCTTC
R3802	T6251	T6242	punct	),552F
R3803	T6252	T6242	cc	and,552F
R3804	T6253	T6242	conj	Ex9RA,552F
R3805	T6254	T6253	punct	(,Ex9RA
R3806	T6255	T6256	nummod	5,GCAGTCTCTGCCTCGTTTTCT
R3807	T6256	T6253	appos	GCAGTCTCTGCCTCGTTTTCT,Ex9RA
R3808	T6257	T6255	punct	',5
R3809	T6258	T6256	punct	-,GCAGTCTCTGCCTCGTTTTCT
R3810	T6259	T6256	punct	-,GCAGTCTCTGCCTCGTTTTCT
R3811	T6260	T6256	nummod	3,GCAGTCTCTGCCTCGTTTTCT
R3812	T6261	T6256	punct	',GCAGTCTCTGCCTCGTTTTCT
R3813	T6262	T6238	punct	),primers
R3814	T6263	T6264	aux	to,yield
R3815	T6264	T6238	advcl	yield,primers
R3816	T6265	T6266	det	a,band
R3817	T6266	T6264	dobj	band,yield
R3818	T6267	T6268	punct	~,500
R3819	T6268	T6269	nummod	500,bp
R3820	T6269	T6266	compound	bp,band
R3821	T6270	T6201	punct	.,was
R3822	T6272	T6273	compound	PCR,cycling
R3823	T6273	T6274	compound	cycling,conditions
R3824	T6274	T6275	nsubj	conditions,were
R3825	T6276	T6277	nummod	95,°C
R3826	T6277	T6275	attr	°C,were
R3827	T6278	T6277	prep	for,°C
R3828	T6279	T6280	nummod	30,seconds
R3829	T6280	T6278	pobj	seconds,for
R3830	T6281	T6277	punct	", ",°C
R3831	T6282	T6283	nummod	58,°C
R3832	T6283	T6277	conj	°C,°C
R3833	T6284	T6283	prep	for,°C
R3834	T6285	T6286	nummod	30,seconds
R3835	T6286	T6284	pobj	seconds,for
R3836	T6287	T6283	punct	", ",°C
R3837	T6288	T6283	cc	and,°C
R3838	T6289	T6290	nummod	72,°C
R3839	T6290	T6283	conj	°C,°C
R3840	T6291	T6290	prep	for,°C
R3841	T6292	T6293	nummod	35,seconds
R3842	T6293	T6291	pobj	seconds,for
R3843	T6294	T6277	punct	", ",°C
R3844	T6295	T6277	acl	repeated,°C
R3845	T6296	T6295	prep	for,repeated
R3846	T6297	T6298	nummod	34,cycles
R3847	T6298	T6296	pobj	cycles,for
R3848	T6299	T6275	punct	.,were
R3849	T6301	T6302	amod	Total,isolation
R3850	T6302	T6304	nsubjpass	isolation,described
R3851	T6303	T6302	compound	RNA,isolation
R3852	T6305	T6302	cc	and,isolation
R3853	T6306	T6307	compound	Cln3,primers
R3854	T6307	T6302	conj	primers,isolation
R3855	T6308	T6309	compound	RT,PCR
R3856	T6309	T6307	compound	PCR,primers
R3857	T6310	T6309	punct	-,PCR
R3858	T6311	T6307	cc	and,primers
R3859	T6312	T6307	conj	procedures,primers
R3860	T6313	T6304	aux	have,described
R3861	T6314	T6304	auxpass	been,described
R3862	T6315	T6304	advmod	previously,described
R3863	T6316	T6317	punct	[,12
R3864	T6317	T6304	parataxis	12,described
R3865	T6318	T6317	punct	],12
R3866	T6319	T6304	punct	.,described
R3877	T6382	T6381	compound	chamber,slides
R3878	T6383	T6381	punct	-,slides
R3879	T6384	T6385	punct	(,Falcon
R3880	T6385	T6381	parataxis	Falcon,slides
R3881	T6386	T6385	punct	),Falcon
R3882	T6387	T6375	prep	at,seeded
R3883	T6388	T6389	det	a,density
R3884	T6389	T6387	pobj	density,at
R3885	T6390	T6389	prep	of,density
R3886	T6391	T6392	quantmod	5,104
R3887	T6392	T6394	nummod	104,cells
R3888	T6393	T6392	punct	×,104
R3889	T6394	T6390	pobj	cells,of
R3890	T6395	T6394	prep	per,cells
R3891	T6396	T6395	pobj	well,per
R3892	T6397	T6375	prep	for,seeded
R3893	T6398	T6399	compound	microscopy,studies
R3894	T6399	T6397	pobj	studies,for
R3895	T6400	T6375	punct	", ",seeded
R3896	T6401	T6375	cc	or,seeded
R3897	T6402	T6375	conj	into,seeded
R3898	T6403	T6404	nummod	100,mm
R3899	T6404	T6405	compound	mm,dishes
R3900	T6405	T6402	pobj	dishes,into
R3901	T6406	T6407	punct	(,Falcon
R3902	T6407	T6405	parataxis	Falcon,dishes
R3903	T6408	T6407	punct	),Falcon
R3904	T6409	T6402	prep	at,into
R3905	T6410	T6411	det	a,density
R3906	T6411	T6409	pobj	density,at
R3907	T6412	T6411	prep	of,density
R3908	T6413	T6414	quantmod	5,105
R3910	T6415	T6414	punct	×,105
R3935	T6442	T6444	npadvmod	day,post-confluency
R3937	T6444	T6439	advmod	post-confluency,considered
R3938	T6445	T6439	punct	.,considered
R3940	T6449	T6450	det	the,times
R3941	T6450	T6447	pobj	times,At
R3942	T6451	T6450	amod	indicated,times
R3943	T6452	T6448	punct	", ",fixed
R4450	T7116	T7117	compound	Cathepsin,D
R4451	T7117	T7118	compound	D,assay
R4452	T7119	T7118	compound	activity,assay
R4492	T7161	T7157	punct	", ",Tris
R4493	T7162	T7163	nummod	0.1,%
R4494	T7163	T7164	compound	%,X
R4495	T7164	T7157	appos	X,Tris
R4496	T7165	T7164	compound	Triton,X
R4497	T7166	T7164	punct	-,X
R4498	T7167	T7164	nummod	100,X
R4499	T7168	T7157	acl	followed,Tris
R4500	T7169	T7168	agent	by,followed
R4501	T7170	T7169	pobj	incubation,by
R4502	T7171	T7170	prep	on,incubation
R4503	T7172	T7171	pobj	ice,on
R4504	T7173	T7170	punct	", ",incubation
R4505	T7174	T7170	prep	for,incubation
R4506	T7175	T7176	nummod	20,minutes
R4507	T7176	T7174	pobj	minutes,for
R4508	T7177	T7149	punct	.,isolated
R4509	T7179	T7180	det	The,material
R4511	T7181	T7180	amod	insoluble,material
R4510	T7180	T7182	nsubjpass	material,centrifuged
R4519	T7190	T7182	conj	isolated,centrifuged
R4521	T7192	T7190	punct	", ",isolated
R4522	T7193	T7190	cc	and,isolated
R3867	T6369	T6370	compound	Subunit,c
R3868	T6370	T6371	compound	c,accumulation
R3869	T6371	T6372	compound	accumulation,assay
R3870	T6374	T6375	nsubjpass	Cells,seeded
R3871	T6376	T6375	auxpass	were,seeded
R3872	T6377	T6375	prep	into,seeded
R3873	T6378	T6379	nummod	4,well
R4594	T7269	T7248	dep	35,measured
R4595	T7270	T7269	punct	", ",35
R4596	T7271	T7269	nummod	360,35
R4597	T7272	T7269	punct	-,35
R4598	T7273	T7269	punct	;,35
R4599	T7274	T7275	compound	emission,filter
R4600	T7275	T7276	dep	filter,20
R4601	T7276	T7269	conj	20,35
R3874	T6379	T6381	compound	well,slides
R3875	T6380	T6379	punct	-,well
R3876	T6381	T6377	pobj	slides,into
R3909	T6414	T6416	nummod	105,cells
R3911	T6416	T6412	pobj	cells,of
R3912	T6417	T6416	prep	per,cells
R3913	T6418	T6417	pobj	dish,per
R3914	T6419	T6402	prep	for,into
R3915	T6420	T6421	compound	protein,extraction
R3916	T6421	T6419	pobj	extraction,for
R3917	T6422	T6375	punct	.,seeded
R3918	T6424	T6425	nsubj	Cells,were
R3919	T6426	T6425	advmod	typically,were
R3920	T6427	T6428	punct	>,95
R3921	T6428	T6429	nummod	95,%
R3922	T6429	T6430	npadvmod	%,confluent
R3923	T6430	T6425	acomp	confluent,were
R3924	T6431	T6432	nummod	one,day
R3925	T6432	T6433	npadvmod	day,post-plating
R3926	T6433	T6425	advmod	post-plating,were
R3927	T6434	T6425	punct	", ",were
R3928	T6435	T6425	cc	and,were
R3929	T6436	T6437	det	the,day
R3930	T6437	T6439	nsubjpass	day,considered
R3931	T6438	T6437	amod	following,day
R3932	T6439	T6425	conj	considered,were
R3933	T6440	T6439	auxpass	was,considered
R3934	T6441	T6442	nummod	1,day
R3936	T6443	T6442	punct	-,day
R4002	T6513	T6510	npadvmod	pH,buffer
R4003	T6514	T6513	nummod	7.4,pH
R4004	T6515	T6495	prep	for,fixed
R4005	T6516	T6517	nummod	1,hour
R4019	T6530	T6529	punct	],12
R4006	T6517	T6515	pobj	hour,for
R4020	T6531	T6495	punct	.,fixed
R4007	T6518	T6495	cc	and,fixed
R3939	T6447	T6448	prep	At,fixed
R4008	T6519	T6520	advmod	subsequently,post-fixed
R4009	T6520	T6495	conj	post-fixed,fixed
R4010	T6521	T6520	cc	and,post-fixed
R4011	T6522	T6520	conj	processed,post-fixed
R4012	T6523	T6522	prep	for,processed
R4013	T6524	T6525	compound	TEM,analysis
R4014	T6525	T6523	pobj	analysis,for
R4015	T6526	T6527	mark	as,described
R4016	T6527	T6522	advcl	described,processed
R4017	T6528	T6529	punct	[,12
R4018	T6529	T6527	parataxis	12,described
R3944	T6453	T6448	nsubjpass	cells,fixed
R3945	T6454	T6448	auxpass	were,fixed
R3946	T6455	T6448	advmod	either,fixed
R3947	T6456	T6448	prep	with,fixed
R3948	T6457	T6458	nummod	4,%
R3949	T6458	T6459	compound	%,formaldehyde
R3950	T6459	T6456	pobj	formaldehyde,with
R3951	T6460	T6448	prep	in,fixed
R3952	T6461	T6462	nmod	phosphate,saline
R3953	T6462	T6460	pobj	saline,in
R3954	T6463	T6462	amod	buffered,saline
R3955	T6464	T6462	punct	(,saline
R3956	T6465	T6462	appos	PBS,saline
R3957	T6466	T6462	punct	),saline
R3958	T6467	T6462	punct	", ",saline
R3959	T6468	T6462	npadvmod	pH,saline
R3960	T6469	T6468	nummod	7.4,pH
R3961	T6470	T6448	punct	", ",fixed
R3962	T6471	T6448	prep	for,fixed
R3963	T6472	T6473	nummod	20,minutes
R3964	T6473	T6471	pobj	minutes,for
R3965	T6474	T6448	cc	and,fixed
R4021	T6533	T6534	prep	In,observed
R4022	T6535	T6536	amod	confocal,studies
R4023	T6536	T6533	pobj	studies,In
R4024	T6537	T6536	compound	microscopy,studies
R4025	T6538	T6534	punct	", ",observed
R4026	T6539	T6540	amod	autofluorescent,signal
R4027	T6540	T6534	nsubjpass	signal,observed
R4028	T6541	T6534	auxpass	was,observed
R4029	T6542	T6534	prep	over,observed
R4030	T6543	T6544	amod	multiple,wavelengths
R4031	T6544	T6542	pobj	wavelengths,over
R4032	T6545	T6534	punct	.,observed
R4033	T6547	T6548	prep	For,reduced
R4034	T6549	T6547	pobj	co-staining,For
R4035	T6550	T6548	punct	", ",reduced
R4036	T6551	T6548	nsubjpass	settings,reduced
R4037	T6552	T6548	auxpass	were,reduced
R4038	T6553	T6548	advcl	such,reduced
R4039	T6554	T6555	mark	that,contribute
R4041	T6556	T6557	amod	autofluorescent,signal
R4040	T6555	T6553	ccomp	contribute,such
R4042	T6557	T6555	nsubj	signal,contribute
R4043	T6558	T6555	aux	did,contribute
R4044	T6559	T6555	neg	not,contribute
R4045	T6560	T6555	prep	to,contribute
R4046	T6561	T6562	compound	antibody,signal
R4047	T6562	T6560	pobj	signal,to
R4048	T6563	T6562	compound	label,signal
R4049	T6564	T6548	punct	.,reduced

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T6623	5106-5111	CL_GO_EXT:cell	denotes	cells
T6624	5150-5155	CL_GO_EXT:cell	denotes	cells
T6625	5194-5199	GO:0040007	denotes	grown
T6626	5277-5289	CHEBI:16842	denotes	formaldehyde
T6627	5339-5347	CHEBI:17790	denotes	methanol
T6628	5348-5355	CHEBI:15347	denotes	acetone
T6629	5410-5418	GO:0042571	denotes	antibody
T6630	5431-5436	CL_GO_EXT:cell	denotes	Cells
T6631	5565-5572	CHEBI_SO_EXT:glycine	denotes	glycine
T6632	5610-5618	GO:0042571	denotes	antibody
T6633	5630-5642	CHEBI:9750	denotes	Triton X-100
T6634	5688-5694	NCBITaxon:27592	denotes	bovine
T6635	5695-5700	UBERON:0001977	denotes	serum
T6636	5695-5708	PR_EXT:000003918	denotes	serum albumin
T6637	5746-5754	GO:0042571	denotes	antibody
T6638	5803-5811	GO:0042571	denotes	antibody
T6639	6121-6130	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T6640	6135-6141	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T6641	6174-6183	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T5190	9-17	GO:0042571	denotes	Antibody
T5191	22-26	CL_GO_EXT:cell	denotes	cell
T5192	36-44	CHEBI_EXT:33893	denotes	reagents
T5193	45-51	PR_EXT:000011141	denotes	Nestin
T5194	53-58	SO_EXT:sequence_cloned_entity	denotes	clone
T5195	59-62	NCBITaxon:10114	denotes	Rat
T5196	78-84	PR_EXT:000002060	denotes	Lamp 1
T5197	86-91	SO_EXT:sequence_cloned_entity	denotes	clone
T5198	112-118	PR_EXT:000002061	denotes	Lamp 2
T5199	120-125	SO_EXT:sequence_cloned_entity	denotes	clone
T5200	154-164	GO:0042571	denotes	antibodies
T5201	278-288	CHEBI_GO_EXT:biological_process_or_quality_or_role	denotes	Biological
T5202	351-360	PR_EXT:000022190	denotes	subunit c
T5203	361-369	GO:0042571	denotes	antibody
T5204	466-476	GO:0042571	denotes	antibodies
T5205	494-498	PR_EXT:000007939	denotes	GFAP
T5206	527-536	PR_EXT:000004967	denotes	calbindin
T5207	554-558	PR_EXT:000006252	denotes	NeuN
T5208	581-585	NCBITaxon:10633	denotes	SV40
T5209	588-595	CHEBI_EXT:59132	denotes	antigen
T5210	643-654	PR_EXT:000006025	denotes	cathepsin D
T5211	699-711	CHEBI:18070	denotes	cytochrome c
T5212	699-719	GO_EXT:0004129	denotes	cytochrome c oxidase
T5213	699-730	PR_EXT:000005776	denotes	cytochrome c oxidase subunit IV
T5214	732-736	PR_EXT:000005776	denotes	cox4
T5215	773-776	GO_PR_EXT:protein_disulfide_isomerase	denotes	PDI
T5216	822-827	PR_EXT:000008131	denotes	GM130
T5217	841-853	GO:0009293	denotes	Transduction
T5218	863-870	PR_EXT:000028799	denotes	tubulin
T5219	890-895	PR_EXT:000008813	denotes	grp75
T5220	916-930	GO:0005769	denotes	early endosome
T5221	916-940	PR_EXT:000006901	denotes	early endosome antigen-1
T5222	931-938	CHEBI_EXT:59132	denotes	antigen
T5223	942-946	PR_EXT:000006901	denotes	EEA1
T5224	985-990	PR_EXT:000013639	denotes	rab 7
T5225	1054-1064	GO:0042571	denotes	antibodies
T5226	1128-1138	MOP:0000779	denotes	conjugated
T5227	1149-1159	GO:0042571	denotes	antibodies
T5228	1212-1216	CL_GO_EXT:cell	denotes	cell
T5229	1217-1224	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	markers
T5230	1246-1252	CHEBI:15956	denotes	biotin
T5231	1294-1301	CHEBI:52071	denotes	dextran
T5232	1302-1306	CHEBI:37926	denotes	FITC
T5233	1320-1342	CHEBI:52117	denotes	Lysotracker Red DND-99
T5234	1352-1361	CHEBI_EXT:polyatomic_entity_or_group	denotes	Molecular
T5235	1362-1368	CHEBI_SO_EXT:molecular_probe	denotes	Probes
T5658	1400-1415	GO_RO_EXT:developmental_differentiation_process	denotes	differentiation
T5659	1419-1421	UBERON:0002037	denotes	Cb
T5660	1421-1425	PR_EXT:000005591	denotes	Cln3
T5661	1426-1436	UBERON:0002037	denotes	cerebellar
T5662	1426-1445	CL:1001611	denotes	cerebellar neuronal
T5663	1462-1466	PR_EXT:000005591	denotes	Cln3
T5664	1482-1486	NCBITaxon:10088	denotes	mice
T5665	1535-1539	NCBITaxon_UBERON_EXT:pup	denotes	pups
T5666	1654-1664	UBERON:0002037	denotes	cerebellar
T5667	1654-1679	CL:0001031	denotes	cerebellar granule neuron
T5668	1708-1713	GO:0007567	denotes	natal
T5669	1725-1734	UBERON:0002037	denotes	cerebella
T5670	1769-1782	CHEBI_EXT:saline_solution	denotes	Salt Solution
T5671	1822-1829	CHEBI:17234	denotes	glucose
T5672	1831-1835	UBERON:0002415	denotes	Tail
T5673	1869-1880	SO_EXT:genomic_DNA	denotes	genomic DNA
T5674	1877-1880	CHEBI_SO_EXT:DNA	denotes	DNA
T5675	1895-1904	SO_EXT:genotype_or_entity_with_genotype	denotes	genotypic
T5676	1915-1922	PR_EXT:000027795	denotes	Trypsin
T5677	1923-1927	CHEBI_EXT:EDTA	denotes	EDTA
T5678	1950-1957	GO_PR_EXT:deoxyribonuclease_I	denotes	DNase I
T5679	2015-2024	UBERON:0002037	denotes	cerebella
T5680	2069-2078	CHEBI_EXT:ornithine	denotes	ornithine
T5681	2142-2151	UBERON:0002037	denotes	cerebella
T5682	2188-2191	CHEBI:16526	denotes	CO2
T5683	2196-2210	CL:0000120	denotes	granule neuron
T5684	2211-2217	GO_EXT:biological_growth_entity_or_process	denotes	growth
T5685	2298-2304	NCBITaxon:27592	denotes	bovine
T5686	2305-2310	UBERON:0001977	denotes	serum
T5687	2352-2355	CHEBI:32588	denotes	KCl
T5688	2415-2425	NCBITaxon:11632	denotes	retrovirus
T5689	2426-2437	GO:0009293	denotes	transducing
T5690	2482-2489	CHEBI_EXT:59132	denotes	antigen
T5691	2506-2514	CHEBI:7507	denotes	neomycin
T5692	2526-2534	SO_EXT:0005853	denotes	cassette
T5693	2642-2648	GO_EXT:biological_growth_entity_or_process	denotes	growth
T5694	2699-2705	GO_EXT:biological_growth_entity_or_process	denotes	growth
T5695	2737-2741	CHEBI:42768	denotes	G418
T5696	2891-2897	SO_EXT:sequence_cloned_entity	denotes	clonal
T5697	2927-2935	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T5698	2996-2998	UBERON:0002037	denotes	Cb
T5699	2998-3002	PR_EXT:000005591	denotes	Cln3
T5700	3003-3007	CL_GO_EXT:cell	denotes	cell
T5701	3015-3017	UBERON:0002037	denotes	Cb
T5702	3022-3026	CL_GO_EXT:cell	denotes	cell
T5703	3063-3072	CHEBI_EXT:ornithine	denotes	ornithine
T5704	3124-3127	CHEBI:16526	denotes	CO2
T5705	3231-3239	CL:0000540	denotes	Neuronal
T5706	3240-3255	GO_RO_EXT:developmental_differentiation_process	denotes	differentiation
T5707	3308-3316	CHEBI:60004	denotes	cocktail
T5708	3327-3332	PR_EXT:000007479	denotes	α-FGF
T5709	3341-3345	CHEBI:48518	denotes	IBMX
T5710	3354-3357	CHEBI:37537	denotes	TPA
T5711	3365-3374	CHEBI:42471	denotes	forskolin
T5712	3381-3389	CHEBI:18243	denotes	dopamine
T6149	3415-3417	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T6150	3422-3433	SO_EXT:genomic_DNA	denotes	Genomic DNA
T6151	3430-3433	CHEBI_SO_EXT:DNA	denotes	DNA
T6152	3453-3457	UBERON:0002415	denotes	tail
T6153	3471-3475	CL_GO_EXT:cell	denotes	cell
T6154	3520-3524	PR_EXT:000005591	denotes	Cln3
T6155	3540-3546	SO_EXT:0001023	denotes	allele
T6156	3571-3580	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T6157	3581-3588	SO_EXT:0000112	denotes	primers
T6158	3678-3680	SO_EXT:0000028	denotes	bp
T6159	3699-3706	SO_EXT:0000112	denotes	primers
T6160	3800-3802	SO_EXT:0000028	denotes	bp
T6161	3934-3937	CHEBI_SO_EXT:RNA	denotes	RNA
T6162	3952-3956	PR_EXT:000005591	denotes	Cln3
T6163	3957-3959	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T6164	3964-3971	SO_EXT:0000112	denotes	primers
T6348	4025-4034	PR_EXT:000022190	denotes	Subunit c
T6349	4054-4059	CL_GO_EXT:cell	denotes	Cells
T6350	4132-4137	CL_GO_EXT:cell	denotes	cells
T6351	4226-4231	CL_GO_EXT:cell	denotes	cells
T6352	4245-4252	CHEBI_PR_EXT:protein	denotes	protein
T6353	4265-4270	CL_GO_EXT:cell	denotes	Cells
T6354	4407-4412	CL_GO_EXT:cell	denotes	cells
T6356	4455-4464	CHEBI_EXT:phosphate	denotes	phosphate
T6357	4465-4473	CHEBI_CHMO_EXT:buffer_process	denotes	buffered
T6358	4474-4480	CHEBI_EXT:saline_solution	denotes	saline
T6359	4546-4555	PR_EXT:000022190	denotes	subunit c
T6360	4575-4579	CL_GO_EXT:cell	denotes	cell
T6361	4613-4620	CHEBI_PR_EXT:protein	denotes	protein
T6362	4648-4653	CL_GO_EXT:cell	denotes	cells
T6363	4675-4689	CHEBI:64276	denotes	glutaraldehyde
T6364	4693-4709	CHEBI_EXT:paraformaldehye	denotes	paraformaldehyde
T6365	4719-4729	CHEBI:16223	denotes	cacodylate
T6366	4730-4736	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T6367	5023-5031	GO:0042571	denotes	antibody
T6368	5032-5037	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	label
T6642	6188-6194	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T6643	6296-6304	CHEBI_PR_EXT:protein	denotes	proteins
T6644	6324-6328	CL_GO_EXT:cell	denotes	cell
T6645	6371-6375	CHEBI:9754	denotes	Tris
T6646	6388-6400	CHEBI:9750	denotes	Triton X-100
T6647	6402-6410	GO:0016020	denotes	membrane
T6648	6440-6459	CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor	denotes	protease inhibitors
T6649	6493-6504	CHEBI:7989	denotes	pepstatin A
T6650	6533-6542	CHEBI:6426	denotes	leupeptin
T6651	6721-6728	CHEBI_PR_EXT:protein	denotes	protein
T6652	6755-6762	CHEBI_PR_EXT:protein	denotes	Protein
T6653	6787-6790	CHEBI:8984	denotes	SDS
T6654	6853-6857	CHEBI:9754	denotes	tris
T6655	6858-6865	CHEBI:46760	denotes	tricine
T6656	6866-6869	CHEBI:8984	denotes	SDS
T6657	6894-6903	PR_EXT:000022190	denotes	subunit c
T7103	6933-6944	PR_EXT:000006025	denotes	Cathepsin D
T7104	6967-6973	UBERON:0000479	denotes	tissue
T7105	7082-7089	CHEBI_PR_EXT:protein	denotes	protein
T7106	7125-7130	CL_GO_EXT:cell	denotes	cells
T7107	7142-7146	CHEBI:9754	denotes	Tris
T7108	7161-7173	CHEBI:9750	denotes	Triton X-100
T7109	7307-7314	CHEBI_PR_EXT:protein	denotes	protein
T7110	7365-7372	CHEBI_PR_EXT:protein	denotes	Protein
T7111	7398-7405	CHEBI_PR_EXT:protein	denotes	protein
T7112	7435-7446	PR_EXT:000006025	denotes	cathepsin D
T7113	7466-7477	CHEBI_EXT:76219	denotes	Fluorogenic
T7114	7488-7499	PR_EXT:000006025	denotes	Cathepsin D
T7115	7669-7678	CHEBI_EXT:polyatomic_entity_or_group	denotes	Molecular
T7323	7875-7884	GO:0005764	denotes	Lysosomal
T7324	7898-7914	GO:0006897	denotes	endocytic uptake
T7325	7915-7920	CL_GO_EXT:cell	denotes	Cells
T7326	7959-7964	CL_GO_EXT:cell	denotes	cells
T7327	8003-8008	GO:0040007	denotes	grown
T7328	8028-8034	GO_EXT:biological_growth_entity_or_process	denotes	Growth
T7329	8077-8083	GO_EXT:biological_growth_entity_or_process	denotes	growth
T7330	8131-8138	CHEBI:52071	denotes	dextran
T7331	8139-8143	CHEBI:37926	denotes	FITC
T7332	8149-8154	CL_GO_EXT:cell	denotes	cells
T7333	8232-8240	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeling
T7334	8242-8247	CL_GO_EXT:cell	denotes	cells
T7335	8317-8320	CHEBI_EXT:37958	denotes	dye
T7336	8351-8363	CHEBI:16842	denotes	formaldehyde
T7480	8558-8570	GO:0005739	denotes	mitochondria
T7481	8714-8726	GO:0005739	denotes	mitochondria
T7482	8784-8796	GO:0005739	denotes	mitochondria
T7483	9060-9073	GO:0005739	denotes	mitochondrial
T7611	9188-9191	CHEBI_EXT:ATP	denotes	ATP
T7612	9204-9207	CHEBI_EXT:ATP	denotes	ATP
T7613	9261-9265	CL_GO_EXT:cell	denotes	Cell
T7614	9349-9354	CL_GO_EXT:cell	denotes	cells
T7615	9521-9528	CHEBI_EXT:33893	denotes	Reagent
T7616	9556-9560	CL_GO_EXT:cell	denotes	cell
T7617	9556-9566	GO:0019835	denotes	cell lysis
T7618	9603-9606	CHEBI_EXT:ATP	denotes	ATP
T7772	9865-9882	CHEBI:16240	denotes	Hydrogen peroxide
T7773	9899-9904	CL_GO_EXT:cell	denotes	Cells
T7774	9940-9945	CL_GO_EXT:cell	denotes	cells
T7775	10070-10087	CHEBI:16240	denotes	hydrogen peroxide
T7776	10116-10121	CL_GO_EXT:cell	denotes	Cells
T7777	10156-10173	CHEBI:16240	denotes	hydrogen peroxide
T7778	10272-10276	CL_GO_EXT:cell	denotes	Cell
T7779	10272-10290	GO:0008283	denotes	Cell Proliferation
T6355	4439-4451	CHEBI:16842	denotes	formaldehyde

2_test

Id	Subject	Object	Predicate	Lexical cue
15588329-12374761-12950161	341-343	12374761	denotes	12
15588329-12374761-12950162	1519-1521	12374761	denotes	12
15588329-8248201-12950163	1699-1701	8248201	denotes	26
15588329-3023876-12950164	2536-2538	3023876	denotes	27
15588329-9671979-12950165	2566-2568	9671979	denotes	28
15588329-11092756-12950166	3285-3287	11092756	denotes	29
15588329-12374761-12950167	4019-4021	12374761	denotes	12
15588329-12374761-12950168	4829-4831	12374761	denotes	12
15588329-12374761-12950169	6842-6844	12374761	denotes	12

PMC:539297 / 25812-36167 JSONTXT

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craft-ca-core-dev

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2_test

PMC:539297 / 25812-36167 JSON TXT