Id |
Subject |
Object |
Predicate |
Lexical cue |
T2526 |
0-10 |
JJ |
denotes |
Homozygous |
T2527 |
24-29 |
NNS |
denotes |
cells |
T2528 |
11-21 |
NN |
denotes |
CbCln3Δex7 |
T2529 |
21-22 |
HYPH |
denotes |
/ |
T2530 |
22-23 |
CD |
denotes |
8 |
T2531 |
59-66 |
VBP |
denotes |
process |
T2532 |
30-33 |
CC |
denotes |
and |
T2533 |
34-42 |
NN |
denotes |
Cln3Δex7 |
T2534 |
54-58 |
NNS |
denotes |
mice |
T2535 |
42-43 |
HYPH |
denotes |
/ |
T2536 |
43-44 |
CD |
denotes |
8 |
T2537 |
45-50 |
VB |
denotes |
knock |
T2538 |
50-51 |
HYPH |
denotes |
- |
T2539 |
51-53 |
RP |
denotes |
in |
T2540 |
67-76 |
NN |
denotes |
cathepsin |
T2541 |
77-78 |
NN |
denotes |
D |
T2542 |
79-89 |
RB |
denotes |
abnormally |
T2543 |
89-282 |
sentence |
denotes |
We next investigated the basis for subunit c accumulation, testing the hypothesis that cathepsin D is abnormal since it is required for ATP synthase subunit c degradation in the lysosome [13]. |
T2544 |
90-92 |
PRP |
denotes |
We |
T2545 |
98-110 |
VBD |
denotes |
investigated |
T2546 |
93-97 |
RB |
denotes |
next |
T2547 |
111-114 |
DT |
denotes |
the |
T2548 |
115-120 |
NN |
denotes |
basis |
T2549 |
121-124 |
IN |
denotes |
for |
T2550 |
125-132 |
NN |
denotes |
subunit |
T2551 |
133-134 |
NN |
denotes |
c |
T2552 |
135-147 |
NN |
denotes |
accumulation |
T2553 |
147-149 |
, |
denotes |
, |
T2554 |
149-156 |
VBG |
denotes |
testing |
T2555 |
157-160 |
DT |
denotes |
the |
T2556 |
161-171 |
NN |
denotes |
hypothesis |
T2557 |
172-176 |
IN |
denotes |
that |
T2558 |
189-191 |
VBZ |
denotes |
is |
T2559 |
177-186 |
NN |
denotes |
cathepsin |
T2560 |
187-188 |
NN |
denotes |
D |
T2561 |
192-200 |
JJ |
denotes |
abnormal |
T2562 |
201-206 |
IN |
denotes |
since |
T2563 |
210-212 |
VBZ |
denotes |
is |
T2564 |
207-209 |
PRP |
denotes |
it |
T2565 |
213-221 |
JJ |
denotes |
required |
T2566 |
222-225 |
IN |
denotes |
for |
T2567 |
226-229 |
NN |
denotes |
ATP |
T2568 |
249-260 |
NN |
denotes |
degradation |
T2569 |
230-238 |
NN |
denotes |
synthase |
T2570 |
239-246 |
NN |
denotes |
subunit |
T2571 |
247-248 |
NN |
denotes |
c |
T2572 |
261-263 |
IN |
denotes |
in |
T2573 |
264-267 |
DT |
denotes |
the |
T2574 |
268-276 |
NN |
denotes |
lysosome |
T2575 |
277-278 |
-LRB- |
denotes |
[ |
T2576 |
278-280 |
CD |
denotes |
13 |
T2577 |
280-281 |
-RRB- |
denotes |
] |
T2578 |
281-282 |
. |
denotes |
. |
T2579 |
282-484 |
sentence |
denotes |
We first tested cathepsin D transport and processing in homozygous CbCln3Δex7/8 cells and Cln3Δex7/8 mice using anti-cathepsin D antibody that recognizes unprocessed and processed cathepsin D isoforms. |
T2580 |
283-285 |
PRP |
denotes |
We |
T2581 |
292-298 |
VBD |
denotes |
tested |
T2582 |
286-291 |
RB |
denotes |
first |
T2583 |
299-308 |
NN |
denotes |
cathepsin |
T2584 |
309-310 |
NN |
denotes |
D |
T2585 |
311-320 |
NN |
denotes |
transport |
T2586 |
321-324 |
CC |
denotes |
and |
T2587 |
325-335 |
NN |
denotes |
processing |
T2588 |
336-338 |
IN |
denotes |
in |
T2589 |
339-349 |
JJ |
denotes |
homozygous |
T2590 |
363-368 |
NNS |
denotes |
cells |
T2591 |
350-360 |
NN |
denotes |
CbCln3Δex7 |
T2592 |
360-361 |
HYPH |
denotes |
/ |
T2593 |
361-362 |
CD |
denotes |
8 |
T2594 |
369-372 |
CC |
denotes |
and |
T2595 |
373-381 |
NN |
denotes |
Cln3Δex7 |
T2596 |
384-388 |
NNS |
denotes |
mice |
T2597 |
381-382 |
HYPH |
denotes |
/ |
T2598 |
382-383 |
CD |
denotes |
8 |
T2599 |
389-394 |
VBG |
denotes |
using |
T2600 |
395-409 |
JJ |
denotes |
anti-cathepsin |
T2601 |
410-411 |
NN |
denotes |
D |
T2602 |
412-420 |
NN |
denotes |
antibody |
T2603 |
421-425 |
WDT |
denotes |
that |
T2604 |
426-436 |
VBZ |
denotes |
recognizes |
T2605 |
437-448 |
JJ |
denotes |
unprocessed |
T2606 |
475-483 |
NNS |
denotes |
isoforms |
T2607 |
449-452 |
CC |
denotes |
and |
T2608 |
453-462 |
VBN |
denotes |
processed |
T2609 |
463-472 |
NN |
denotes |
cathepsin |
T2610 |
473-474 |
NN |
denotes |
D |
T2611 |
483-484 |
. |
denotes |
. |
T2612 |
484-733 |
sentence |
denotes |
Immunostaining of wild-type and homozygous CbCln3Δex7/8 cells revealed a perinuclear and punctate vesicular cathepsin D distribution, consistent with its transport and processing through the secretory pathway and delivery to the lysosome (Fig. 5a). |
T2613 |
485-499 |
NN |
denotes |
Immunostaining |
T2614 |
547-555 |
VBD |
denotes |
revealed |
T2615 |
500-502 |
IN |
denotes |
of |
T2616 |
503-507 |
JJ |
denotes |
wild |
T2617 |
508-512 |
NN |
denotes |
type |
T2618 |
507-508 |
HYPH |
denotes |
- |
T2619 |
541-546 |
NNS |
denotes |
cells |
T2620 |
513-516 |
CC |
denotes |
and |
T2621 |
517-527 |
JJ |
denotes |
homozygous |
T2622 |
528-538 |
NN |
denotes |
CbCln3Δex7 |
T2623 |
538-539 |
HYPH |
denotes |
/ |
T2624 |
539-540 |
CD |
denotes |
8 |
T2625 |
556-557 |
DT |
denotes |
a |
T2626 |
605-617 |
NN |
denotes |
distribution |
T2627 |
558-569 |
JJ |
denotes |
perinuclear |
T2628 |
570-573 |
CC |
denotes |
and |
T2629 |
574-582 |
JJ |
denotes |
punctate |
T2630 |
583-592 |
JJ |
denotes |
vesicular |
T2631 |
593-602 |
NN |
denotes |
cathepsin |
T2632 |
603-604 |
NN |
denotes |
D |
T2633 |
617-619 |
, |
denotes |
, |
T2634 |
619-629 |
JJ |
denotes |
consistent |
T2635 |
630-634 |
IN |
denotes |
with |
T2636 |
635-638 |
PRP$ |
denotes |
its |
T2637 |
639-648 |
NN |
denotes |
transport |
T2638 |
649-652 |
CC |
denotes |
and |
T2639 |
653-663 |
NN |
denotes |
processing |
T2640 |
664-671 |
IN |
denotes |
through |
T2641 |
672-675 |
DT |
denotes |
the |
T2642 |
686-693 |
NN |
denotes |
pathway |
T2643 |
676-685 |
JJ |
denotes |
secretory |
T2644 |
694-697 |
CC |
denotes |
and |
T2645 |
698-706 |
NN |
denotes |
delivery |
T2646 |
707-709 |
IN |
denotes |
to |
T2647 |
710-713 |
DT |
denotes |
the |
T2648 |
714-722 |
NN |
denotes |
lysosome |
T2649 |
723-724 |
-LRB- |
denotes |
( |
T2650 |
729-731 |
NN |
denotes |
5a |
T2651 |
724-728 |
NN |
denotes |
Fig. |
T2652 |
731-732 |
-RRB- |
denotes |
) |
T2653 |
732-733 |
. |
denotes |
. |
T2654 |
733-876 |
sentence |
denotes |
However, in homozygous CbCln3Δex7/8 cells, cathepsin D distribution was less vesicular and more perinuclear-clustered than in wild-type cells. |
T2655 |
734-741 |
RB |
denotes |
However |
T2656 |
802-805 |
VBD |
denotes |
was |
T2657 |
741-743 |
, |
denotes |
, |
T2658 |
743-745 |
IN |
denotes |
in |
T2659 |
746-756 |
JJ |
denotes |
homozygous |
T2660 |
770-775 |
NNS |
denotes |
cells |
T2661 |
757-767 |
NN |
denotes |
CbCln3Δex7 |
T2662 |
767-768 |
HYPH |
denotes |
/ |
T2663 |
768-769 |
CD |
denotes |
8 |
T2664 |
775-777 |
, |
denotes |
, |
T2665 |
777-786 |
NN |
denotes |
cathepsin |
T2666 |
787-788 |
NN |
denotes |
D |
T2667 |
789-801 |
NN |
denotes |
distribution |
T2668 |
806-810 |
RBR |
denotes |
less |
T2669 |
811-820 |
JJ |
denotes |
vesicular |
T2670 |
821-824 |
CC |
denotes |
and |
T2671 |
825-829 |
RBR |
denotes |
more |
T2672 |
842-851 |
VBN |
denotes |
clustered |
T2673 |
830-841 |
JJ |
denotes |
perinuclear |
T2674 |
841-842 |
HYPH |
denotes |
- |
T2675 |
852-856 |
IN |
denotes |
than |
T2676 |
857-859 |
IN |
denotes |
in |
T2677 |
860-864 |
JJ |
denotes |
wild |
T2678 |
865-869 |
NN |
denotes |
type |
T2679 |
864-865 |
HYPH |
denotes |
- |
T2680 |
870-875 |
NNS |
denotes |
cells |
T2681 |
875-876 |
. |
denotes |
. |
T2682 |
876-1022 |
sentence |
denotes |
Immunoblots of homozygous CbCln3Δex7/8 cell and Cln3Δex7/8 tissue extracts also showed altered relative levels of cathepsin D isoforms (Fig. 5b). |
T2683 |
877-888 |
NNS |
denotes |
Immunoblots |
T2684 |
957-963 |
VBD |
denotes |
showed |
T2685 |
889-891 |
IN |
denotes |
of |
T2686 |
892-902 |
JJ |
denotes |
homozygous |
T2687 |
943-951 |
NNS |
denotes |
extracts |
T2688 |
903-913 |
NN |
denotes |
CbCln3Δex7 |
T2689 |
916-920 |
NN |
denotes |
cell |
T2690 |
913-914 |
HYPH |
denotes |
/ |
T2691 |
914-915 |
CD |
denotes |
8 |
T2692 |
921-924 |
CC |
denotes |
and |
T2693 |
925-933 |
NN |
denotes |
Cln3Δex7 |
T2694 |
936-942 |
NN |
denotes |
tissue |
T2695 |
933-934 |
HYPH |
denotes |
/ |
T2696 |
934-935 |
CD |
denotes |
8 |
T2697 |
952-956 |
RB |
denotes |
also |
T2698 |
964-971 |
VBN |
denotes |
altered |
T2699 |
981-987 |
NNS |
denotes |
levels |
T2700 |
972-980 |
JJ |
denotes |
relative |
T2701 |
988-990 |
IN |
denotes |
of |
T2702 |
991-1000 |
NN |
denotes |
cathepsin |
T2703 |
1001-1002 |
NN |
denotes |
D |
T2704 |
1003-1011 |
NNS |
denotes |
isoforms |
T2705 |
1012-1013 |
-LRB- |
denotes |
( |
T2706 |
1018-1020 |
NN |
denotes |
5b |
T2707 |
1013-1017 |
NN |
denotes |
Fig. |
T2708 |
1020-1021 |
-RRB- |
denotes |
) |
T2709 |
1021-1022 |
. |
denotes |
. |
T2710 |
1022-1256 |
sentence |
denotes |
Cathepsin D isoforms, identified by relative molecular weights, represent the ~45 kDa precursor, the ~43 kDa intermediate single chain form of the enzyme, and the 31 kDa heavy chain of the double-chain form of the mature enzyme [14]. |
T2711 |
1023-1032 |
NN |
denotes |
Cathepsin |
T2712 |
1033-1034 |
NN |
denotes |
D |
T2713 |
1035-1043 |
NNS |
denotes |
isoforms |
T2714 |
1087-1096 |
VBP |
denotes |
represent |
T2715 |
1043-1045 |
, |
denotes |
, |
T2716 |
1045-1055 |
VBN |
denotes |
identified |
T2717 |
1056-1058 |
IN |
denotes |
by |
T2718 |
1059-1067 |
JJ |
denotes |
relative |
T2719 |
1078-1085 |
NNS |
denotes |
weights |
T2720 |
1068-1077 |
JJ |
denotes |
molecular |
T2721 |
1085-1087 |
, |
denotes |
, |
T2722 |
1097-1100 |
DT |
denotes |
the |
T2723 |
1109-1118 |
NN |
denotes |
precursor |
T2724 |
1101-1102 |
SYM |
denotes |
~ |
T2725 |
1102-1104 |
CD |
denotes |
45 |
T2726 |
1105-1108 |
NN |
denotes |
kDa |
T2727 |
1118-1120 |
, |
denotes |
, |
T2728 |
1120-1123 |
DT |
denotes |
the |
T2729 |
1158-1162 |
NN |
denotes |
form |
T2730 |
1124-1125 |
SYM |
denotes |
~ |
T2731 |
1125-1127 |
CD |
denotes |
43 |
T2732 |
1128-1131 |
NN |
denotes |
kDa |
T2733 |
1132-1144 |
JJ |
denotes |
intermediate |
T2734 |
1145-1151 |
JJ |
denotes |
single |
T2735 |
1152-1157 |
NN |
denotes |
chain |
T2736 |
1163-1165 |
IN |
denotes |
of |
T2737 |
1166-1169 |
DT |
denotes |
the |
T2738 |
1170-1176 |
NN |
denotes |
enzyme |
T2739 |
1176-1178 |
, |
denotes |
, |
T2740 |
1178-1181 |
CC |
denotes |
and |
T2741 |
1182-1185 |
DT |
denotes |
the |
T2742 |
1199-1204 |
NN |
denotes |
chain |
T2743 |
1186-1188 |
CD |
denotes |
31 |
T2744 |
1189-1192 |
NN |
denotes |
kDa |
T2745 |
1193-1198 |
JJ |
denotes |
heavy |
T2746 |
1205-1207 |
IN |
denotes |
of |
T2747 |
1208-1211 |
DT |
denotes |
the |
T2748 |
1225-1229 |
NN |
denotes |
form |
T2749 |
1212-1218 |
JJ |
denotes |
double |
T2750 |
1219-1224 |
NN |
denotes |
chain |
T2751 |
1218-1219 |
HYPH |
denotes |
- |
T2752 |
1230-1232 |
IN |
denotes |
of |
T2753 |
1233-1236 |
DT |
denotes |
the |
T2754 |
1244-1250 |
NN |
denotes |
enzyme |
T2755 |
1237-1243 |
JJ |
denotes |
mature |
T2756 |
1251-1252 |
-LRB- |
denotes |
[ |
T2757 |
1252-1254 |
CD |
denotes |
14 |
T2758 |
1254-1255 |
-RRB- |
denotes |
] |
T2759 |
1255-1256 |
. |
denotes |
. |
T2760 |
1256-1440 |
sentence |
denotes |
In homozygous CbCln3Δex7/8 cell and Cln3Δex7/8 tissue extracts, the precursor and heavy chains were reduced, and the single chain was slightly elevated compared to wild-type extracts. |
T2761 |
1257-1259 |
IN |
denotes |
In |
T2762 |
1357-1364 |
VBN |
denotes |
reduced |
T2763 |
1260-1270 |
JJ |
denotes |
homozygous |
T2764 |
1311-1319 |
NNS |
denotes |
extracts |
T2765 |
1271-1281 |
NN |
denotes |
CbCln3Δex7 |
T2766 |
1284-1288 |
NN |
denotes |
cell |
T2767 |
1281-1282 |
HYPH |
denotes |
/ |
T2768 |
1282-1283 |
CD |
denotes |
8 |
T2769 |
1289-1292 |
CC |
denotes |
and |
T2770 |
1293-1301 |
NN |
denotes |
Cln3Δex7 |
T2771 |
1304-1310 |
NN |
denotes |
tissue |
T2772 |
1301-1302 |
HYPH |
denotes |
/ |
T2773 |
1302-1303 |
CD |
denotes |
8 |
T2774 |
1319-1321 |
, |
denotes |
, |
T2775 |
1321-1324 |
DT |
denotes |
the |
T2776 |
1325-1334 |
NN |
denotes |
precursor |
T2777 |
1335-1338 |
CC |
denotes |
and |
T2778 |
1339-1344 |
JJ |
denotes |
heavy |
T2779 |
1345-1351 |
NNS |
denotes |
chains |
T2780 |
1352-1356 |
VBD |
denotes |
were |
T2781 |
1364-1366 |
, |
denotes |
, |
T2782 |
1366-1369 |
CC |
denotes |
and |
T2783 |
1370-1373 |
DT |
denotes |
the |
T2784 |
1381-1386 |
NN |
denotes |
chain |
T2785 |
1374-1380 |
JJ |
denotes |
single |
T2786 |
1387-1390 |
VBD |
denotes |
was |
T2787 |
1391-1399 |
RB |
denotes |
slightly |
T2788 |
1400-1408 |
JJ |
denotes |
elevated |
T2789 |
1409-1417 |
VBN |
denotes |
compared |
T2790 |
1418-1420 |
IN |
denotes |
to |
T2838 |
1667-1668 |
DT |
denotes |
a |
T2839 |
1687-1696 |
NN |
denotes |
phenotype |
T2840 |
1669-1678 |
JJ |
denotes |
recessive |
T2841 |
1679-1686 |
NN |
denotes |
disease |
T2842 |
1697-1698 |
-LRB- |
denotes |
( |
T2843 |
1707-1712 |
VBN |
denotes |
shown |
T2844 |
1698-1702 |
NNS |
denotes |
data |
T2845 |
1703-1706 |
RB |
denotes |
not |
T2846 |
1712-1713 |
-RRB- |
denotes |
) |
T2847 |
1713-1714 |
. |
denotes |
. |
T2848 |
1714-1715 |
sentence |
denotes |
|
T2889 |
2960-2969 |
JJ |
denotes |
enzymatic |
T2890 |
2988-2991 |
IN |
denotes |
for |
T2891 |
2992-3003 |
JJ |
denotes |
inefficient |
T2892 |
3014-3022 |
NN |
denotes |
turnover |
T2893 |
3004-3011 |
NN |
denotes |
subunit |
T2894 |
3012-3013 |
NN |
denotes |
c |
T2895 |
3022-3023 |
. |
denotes |
. |
T2896 |
3023-3351 |
sentence |
denotes |
In a fluorogenic in vitro assay, cathepsin D activity in total cellular extracts was not significantly altered in homozygous CbCln3Δex7/8 cells (376 ± 89 RFU/μg total protein), versus wild-type cells (324 ± 58 RFU/μg total protein), although a consistent trend towards increased enzymatic activity in mutant cells was observed. |
T2897 |
3024-3026 |
IN |
denotes |
In |
T2898 |
3127-3134 |
VBN |
denotes |
altered |
T2899 |
3027-3028 |
DT |
denotes |
a |
T2900 |
3050-3055 |
NN |
denotes |
assay |
T2901 |
3029-3040 |
JJ |
denotes |
fluorogenic |
T2902 |
3041-3043 |
FW |
denotes |
in |
T2903 |
3044-3049 |
FW |
denotes |
vitro |
T2904 |
3055-3057 |
, |
denotes |
, |
T2905 |
3057-3066 |
NN |
denotes |
cathepsin |
T2906 |
3067-3068 |
NN |
denotes |
D |
T2907 |
3069-3077 |
NN |
denotes |
activity |
T2908 |
3078-3080 |
IN |
denotes |
in |
T2909 |
3081-3086 |
JJ |
denotes |
total |
T2910 |
3096-3104 |
NNS |
denotes |
extracts |
T2911 |
3087-3095 |
JJ |
denotes |
cellular |
T2912 |
3105-3108 |
VBD |
denotes |
was |
T2913 |
3109-3112 |
RB |
denotes |
not |
T2914 |
3113-3126 |
RB |
denotes |
significantly |
T2915 |
3135-3137 |
IN |
denotes |
in |
T2916 |
3138-3148 |
JJ |
denotes |
homozygous |
T2917 |
3162-3167 |
NNS |
denotes |
cells |
T2918 |
3149-3159 |
NN |
denotes |
CbCln3Δex7 |
T2919 |
3159-3160 |
HYPH |
denotes |
/ |
T2920 |
3160-3161 |
CD |
denotes |
8 |
T2921 |
3168-3169 |
-LRB- |
denotes |
( |
T2922 |
3178-3181 |
NN |
denotes |
RFU |
T2923 |
3169-3172 |
CD |
denotes |
376 |
T2924 |
3175-3177 |
CD |
denotes |
89 |
T2925 |
3173-3174 |
SYM |
denotes |
± |
T2926 |
3181-3182 |
SYM |
denotes |
/ |
T2927 |
3191-3198 |
NN |
denotes |
protein |
T2928 |
3182-3184 |
NN |
denotes |
μg |
T2929 |
3185-3190 |
JJ |
denotes |
total |
T2930 |
3198-3199 |
-RRB- |
denotes |
) |
T2931 |
3199-3201 |
, |
denotes |
, |
T2932 |
3201-3207 |
CC |
denotes |
versus |
T2933 |
3208-3212 |
JJ |
denotes |
wild |
T2934 |
3213-3217 |
NN |
denotes |
type |
T2935 |
3212-3213 |
HYPH |
denotes |
- |
T2936 |
3218-3223 |
NNS |
denotes |
cells |
T2937 |
3224-3225 |
-LRB- |
denotes |
( |
T2938 |
3234-3237 |
NN |
denotes |
RFU |
T2939 |
3225-3228 |
CD |
denotes |
324 |
T2940 |
3231-3233 |
CD |
denotes |
58 |
T2941 |
3229-3230 |
IN |
denotes |
± |
T2942 |
3237-3238 |
SYM |
denotes |
/ |
T2943 |
3247-3254 |
NN |
denotes |
protein |
T2944 |
3238-3240 |
NN |
denotes |
μg |
T2945 |
3241-3246 |
JJ |
denotes |
total |
T2946 |
3254-3255 |
-RRB- |
denotes |
) |
T2947 |
3255-3257 |
, |
denotes |
, |
T2948 |
3257-3265 |
IN |
denotes |
although |
T2949 |
3342-3350 |
VBN |
denotes |
observed |
T2950 |
3266-3267 |
DT |
denotes |
a |
T2951 |
3279-3284 |
NN |
denotes |
trend |
T2952 |
3268-3278 |
JJ |
denotes |
consistent |
T2953 |
3285-3292 |
IN |
denotes |
towards |
T2954 |
3293-3302 |
VBN |
denotes |
increased |
T2955 |
3313-3321 |
NN |
denotes |
activity |
T2956 |
3303-3312 |
JJ |
denotes |
enzymatic |
T2957 |
3322-3324 |
IN |
denotes |
in |
T2958 |
3325-3331 |
NN |
denotes |
mutant |
T2959 |
3332-3337 |
NNS |
denotes |
cells |
T2960 |
3338-3341 |
VBD |
denotes |
was |
T2961 |
3350-3351 |
. |
denotes |
. |
T2962 |
3351-3519 |
sentence |
denotes |
Thus, cathepsin D transport and processing are disrupted in homozygous CbCln3Δex7/8 cells in a manner such that enzymatic activity appears to be relatively unaffected. |
T2963 |
3352-3356 |
RB |
denotes |
Thus |
T2964 |
3399-3408 |
VBN |
denotes |
disrupted |
T2965 |
3356-3358 |
, |
denotes |
, |
T2966 |
3358-3367 |
NN |
denotes |
cathepsin |
T2967 |
3368-3369 |
NN |
denotes |
D |
T2968 |
3370-3379 |
NN |
denotes |
transport |
T2969 |
3380-3383 |
CC |
denotes |
and |
T2970 |
3384-3394 |
NN |
denotes |
processing |
T2971 |
3395-3398 |
VBP |
denotes |
are |
T2972 |
3409-3411 |
IN |
denotes |
in |
T2973 |
3412-3422 |
JJ |
denotes |
homozygous |
T2974 |
3436-3441 |
NNS |
denotes |
cells |
T2975 |
3423-3433 |
NN |
denotes |
CbCln3Δex7 |
T2976 |
3433-3434 |
HYPH |
denotes |
/ |
T2977 |
3434-3435 |
CD |
denotes |
8 |
T2978 |
3442-3444 |
IN |
denotes |
in |
T2979 |
3445-3446 |
DT |
denotes |
a |
T2980 |
3447-3453 |
NN |
denotes |
manner |
T2981 |
3454-3458 |
JJ |
denotes |
such |
T2982 |
3459-3463 |
IN |
denotes |
that |
T2983 |
3483-3490 |
VBZ |
denotes |
appears |
T2984 |
3464-3473 |
JJ |
denotes |
enzymatic |
T2985 |
3474-3482 |
NN |
denotes |
activity |
T2986 |
3491-3493 |
TO |
denotes |
to |
T2987 |
3494-3496 |
VB |
denotes |
be |
T2988 |
3497-3507 |
RB |
denotes |
relatively |
T2989 |
3508-3518 |
JJ |
denotes |
unaffected |
T2990 |
3518-3519 |
. |
denotes |
. |
T9254 |
1725-1734 |
NN |
denotes |
Cathepsin |
T9255 |
1735-1736 |
NN |
denotes |
D |
T9256 |
1737-1749 |
NN |
denotes |
localization |
T9257 |
1750-1753 |
CC |
denotes |
and |
T9258 |
1754-1764 |
NN |
denotes |
processing |
T9259 |
1765-1767 |
IN |
denotes |
in |
T9260 |
1768-1772 |
JJ |
denotes |
wild |
T9261 |
1773-1777 |
NN |
denotes |
type |
T9262 |
1772-1773 |
HYPH |
denotes |
- |
T9263 |
1806-1811 |
NNS |
denotes |
cells |
T9264 |
1778-1781 |
CC |
denotes |
and |
T9265 |
1782-1792 |
JJ |
denotes |
homozygous |
T9266 |
1793-1803 |
NN |
denotes |
CbCln3Δex7 |
T9267 |
1803-1804 |
HYPH |
denotes |
/ |
T9268 |
1804-1805 |
CD |
denotes |
8 |
T9269 |
1811-1995 |
sentence |
denotes |
a. Immunostaining of wild-type and homozygous CbCln3Δex7/8 precursor cells with anti-cathepsin D antibody, recognizing unprocessed and processed forms of cathepsin D protein is shown. |
T9270 |
1812-1813 |
LS |
denotes |
a |
T9271 |
1989-1994 |
VBN |
denotes |
shown |
T9272 |
1813-1814 |
. |
denotes |
. |
T9273 |
1815-1829 |
NN |
denotes |
Immunostaining |
T9274 |
1830-1832 |
IN |
denotes |
of |
T9275 |
1833-1837 |
JJ |
denotes |
wild |
T9276 |
1838-1842 |
NN |
denotes |
type |
T9277 |
1837-1838 |
HYPH |
denotes |
- |
T9278 |
1881-1886 |
NNS |
denotes |
cells |
T9279 |
1843-1846 |
CC |
denotes |
and |
T9280 |
1847-1857 |
JJ |
denotes |
homozygous |
T9281 |
1858-1868 |
NN |
denotes |
CbCln3Δex7 |
T9282 |
1868-1869 |
HYPH |
denotes |
/ |
T9283 |
1869-1870 |
CD |
denotes |
8 |
T9284 |
1871-1880 |
NN |
denotes |
precursor |
T9285 |
1887-1891 |
IN |
denotes |
with |
T9286 |
1892-1906 |
JJ |
denotes |
anti-cathepsin |
T9287 |
1907-1908 |
NN |
denotes |
D |
T9288 |
1909-1917 |
NN |
denotes |
antibody |
T9289 |
1917-1919 |
, |
denotes |
, |
T9290 |
1919-1930 |
VBG |
denotes |
recognizing |
T9291 |
1931-1942 |
JJ |
denotes |
unprocessed |
T9292 |
1957-1962 |
NNS |
denotes |
forms |
T9293 |
1943-1946 |
CC |
denotes |
and |
T9294 |
1947-1956 |
VBN |
denotes |
processed |
T9295 |
1963-1965 |
IN |
denotes |
of |
T9296 |
1966-1975 |
NN |
denotes |
cathepsin |
T9297 |
1976-1977 |
NN |
denotes |
D |
T9298 |
1978-1985 |
NN |
denotes |
protein |
T9299 |
1986-1988 |
VBZ |
denotes |
is |
T9300 |
1994-1995 |
. |
denotes |
. |
T9301 |
1995-2081 |
sentence |
denotes |
CbCln3+/+ cells (left panel) exhibited a perinuclear and cytoplasmic punctate signal. |
T9302 |
1996-2002 |
NN |
denotes |
CbCln3 |
T9303 |
2006-2011 |
NNS |
denotes |
cells |
T9304 |
2002-2003 |
SYM |
denotes |
+ |
T9305 |
2003-2004 |
HYPH |
denotes |
/ |
T9306 |
2004-2005 |
SYM |
denotes |
+ |
T9307 |
2025-2034 |
VBD |
denotes |
exhibited |
T9308 |
2012-2013 |
-LRB- |
denotes |
( |
T9309 |
2018-2023 |
NN |
denotes |
panel |
T9310 |
2013-2017 |
JJ |
denotes |
left |
T9311 |
2023-2024 |
-RRB- |
denotes |
) |
T9312 |
2035-2036 |
DT |
denotes |
a |
T9313 |
2074-2080 |
NN |
denotes |
signal |
T9314 |
2037-2048 |
JJ |
denotes |
perinuclear |
T9315 |
2049-2052 |
CC |
denotes |
and |
T9316 |
2053-2064 |
JJ |
denotes |
cytoplasmic |
T9317 |
2065-2073 |
JJ |
denotes |
punctate |
T9318 |
2080-2081 |
. |
denotes |
. |
T9319 |
2081-2253 |
sentence |
denotes |
Cathepsin D signal in homozygous CbCln3Δex7/8 cells (right panel) was more often perinuclear, with less cytoplasmic punctate signal, compared to wild-type CbCln3+/+ cells. |
T9320 |
2082-2091 |
NN |
denotes |
Cathepsin |
T9321 |
2092-2093 |
NN |
denotes |
D |
T9322 |
2094-2100 |
NN |
denotes |
signal |
T9323 |
2148-2151 |
VBD |
denotes |
was |
T9324 |
2101-2103 |
IN |
denotes |
in |
T9325 |
2104-2114 |
JJ |
denotes |
homozygous |
T9326 |
2128-2133 |
NNS |
denotes |
cells |
T9327 |
2115-2125 |
NN |
denotes |
CbCln3Δex7 |
T9328 |
2125-2126 |
HYPH |
denotes |
/ |
T9329 |
2126-2127 |
CD |
denotes |
8 |
T9330 |
2134-2135 |
-LRB- |
denotes |
( |
T9331 |
2141-2146 |
NN |
denotes |
panel |
T9332 |
2135-2140 |
JJ |
denotes |
right |
T9333 |
2146-2147 |
-RRB- |
denotes |
) |
T9334 |
2152-2156 |
RBR |
denotes |
more |
T9335 |
2157-2162 |
RB |
denotes |
often |
T9336 |
2163-2174 |
JJ |
denotes |
perinuclear |
T9337 |
2174-2176 |
, |
denotes |
, |
T9338 |
2176-2180 |
IN |
denotes |
with |
T9339 |
2181-2185 |
RBR |
denotes |
less |
T9340 |
2207-2213 |
NN |
denotes |
signal |
T9341 |
2186-2197 |
JJ |
denotes |
cytoplasmic |
T9342 |
2198-2206 |
NN |
denotes |
punctate |
T9343 |
2213-2215 |
, |
denotes |
, |
T9344 |
2215-2223 |
VBN |
denotes |
compared |
T9345 |
2224-2226 |
IN |
denotes |
to |
T9346 |
2227-2231 |
JJ |
denotes |
wild |
T9347 |
2232-2236 |
NN |
denotes |
type |
T9348 |
2231-2232 |
HYPH |
denotes |
- |
T9349 |
2247-2252 |
NNS |
denotes |
cells |
T9350 |
2237-2243 |
NN |
denotes |
CbCln3 |
T9351 |
2243-2244 |
SYM |
denotes |
+ |
T9352 |
2244-2245 |
HYPH |
denotes |
/ |
T9353 |
2245-2246 |
SYM |
denotes |
+ |
T9354 |
2252-2253 |
. |
denotes |
. |
T9355 |
2253-2273 |
sentence |
denotes |
40 × magnification. |
T9356 |
2254-2256 |
CD |
denotes |
40 |
T9357 |
2259-2272 |
NN |
denotes |
magnification |
T9358 |
2257-2258 |
SYM |
denotes |
× |
T9359 |
2272-2273 |
. |
denotes |
. |
T9360 |
2273-2418 |
sentence |
denotes |
b. α-Cathepsin D-probed immunoblots of total wild-type versus homozygous Cln3Δex7/8 knock-in tissue or CbCln3Δex7/8 cellular extracts are shown. |
T9361 |
2274-2275 |
LS |
denotes |
b |
T9362 |
2412-2417 |
VBN |
denotes |
shown |
T9363 |
2275-2276 |
. |
denotes |
. |
T9364 |
2277-2278 |
NN |
denotes |
α |
T9365 |
2289-2290 |
NN |
denotes |
D |
T9366 |
2278-2279 |
HYPH |
denotes |
- |
T9367 |
2279-2288 |
NN |
denotes |
Cathepsin |
T9368 |
2291-2297 |
VBN |
denotes |
probed |
T9369 |
2290-2291 |
HYPH |
denotes |
- |
T9370 |
2298-2309 |
NNS |
denotes |
immunoblots |
T9371 |
2310-2312 |
IN |
denotes |
of |
T9372 |
2313-2318 |
JJ |
denotes |
total |
T9373 |
2367-2373 |
NN |
denotes |
tissue |
T9374 |
2319-2323 |
JJ |
denotes |
wild |
T9375 |
2324-2328 |
NN |
denotes |
type |
T9376 |
2323-2324 |
HYPH |
denotes |
- |
T9377 |
2329-2335 |
CC |
denotes |
versus |
T9378 |
2336-2346 |
JJ |
denotes |
homozygous |
T9379 |
2347-2355 |
NN |
denotes |
Cln3Δex7 |
T9380 |
2358-2363 |
VB |
denotes |
knock |
T9381 |
2355-2356 |
HYPH |
denotes |
/ |
T9382 |
2356-2357 |
CD |
denotes |
8 |
T9383 |
2363-2364 |
HYPH |
denotes |
- |
T9384 |
2364-2366 |
RP |
denotes |
in |
T9385 |
2374-2376 |
CC |
denotes |
or |
T9386 |
2377-2387 |
NN |
denotes |
CbCln3Δex7 |
T9387 |
2399-2407 |
NNS |
denotes |
extracts |
T9388 |
2387-2388 |
HYPH |
denotes |
/ |
T9389 |
2388-2389 |
CD |
denotes |
8 |
T9390 |
2390-2398 |
JJ |
denotes |
cellular |
T9391 |
2408-2411 |
VBP |
denotes |
are |
T9392 |
2417-2418 |
. |
denotes |
. |
T9393 |
2418-2631 |
sentence |
denotes |
The ~45 kDa cathepsin D band, representing precursor, was the predominant band in wild-type (wt) tissue and cellular extracts, with lower levels of mature enzyme (single chain, ~43 kDa, and heavy chain, ~31 kDa). |
T9394 |
2419-2422 |
DT |
denotes |
The |
T9395 |
2443-2447 |
NN |
denotes |
band |
T9396 |
2423-2424 |
SYM |
denotes |
~ |
T9397 |
2424-2426 |
CD |
denotes |
45 |
T9398 |
2427-2430 |
NN |
denotes |
kDa |
T9399 |
2431-2440 |
NN |
denotes |
cathepsin |
T9400 |
2441-2442 |
NN |
denotes |
D |
T9401 |
2473-2476 |
VBD |
denotes |
was |
T9402 |
2447-2449 |
, |
denotes |
, |
T9403 |
2449-2461 |
VBG |
denotes |
representing |
T9404 |
2462-2471 |
NN |
denotes |
precursor |
T9405 |
2471-2473 |
, |
denotes |
, |
T9406 |
2477-2480 |
DT |
denotes |
the |
T9407 |
2493-2497 |
NN |
denotes |
band |
T9408 |
2481-2492 |
JJ |
denotes |
predominant |
T9409 |
2498-2500 |
IN |
denotes |
in |
T9410 |
2501-2505 |
JJ |
denotes |
wild |
T9411 |
2506-2510 |
NN |
denotes |
type |
T9412 |
2505-2506 |
HYPH |
denotes |
- |
T9413 |
2511-2512 |
-LRB- |
denotes |
( |
T9414 |
2512-2514 |
NN |
denotes |
wt |
T9415 |
2514-2515 |
-RRB- |
denotes |
) |
T9416 |
2516-2522 |
NN |
denotes |
tissue |
T9417 |
2523-2526 |
CC |
denotes |
and |
T9418 |
2527-2535 |
JJ |
denotes |
cellular |
T9419 |
2536-2544 |
NNS |
denotes |
extracts |
T9420 |
2544-2546 |
, |
denotes |
, |
T9421 |
2546-2550 |
IN |
denotes |
with |
T9422 |
2551-2556 |
JJR |
denotes |
lower |
T9423 |
2557-2563 |
NNS |
denotes |
levels |
T9424 |
2564-2566 |
IN |
denotes |
of |
T9425 |
2567-2573 |
JJ |
denotes |
mature |
T9426 |
2574-2580 |
NN |
denotes |
enzyme |
T9427 |
2581-2582 |
-LRB- |
denotes |
( |
T9428 |
2600-2603 |
NN |
denotes |
kDa |
T9429 |
2582-2588 |
JJ |
denotes |
single |
T9430 |
2589-2594 |
NN |
denotes |
chain |
T9431 |
2594-2596 |
, |
denotes |
, |
T9432 |
2596-2597 |
SYM |
denotes |
~ |
T9433 |
2597-2599 |
CD |
denotes |
43 |
T9434 |
2603-2605 |
, |
denotes |
, |
T9435 |
2605-2608 |
CC |
denotes |
and |
T9436 |
2609-2614 |
JJ |
denotes |
heavy |
T9437 |
2615-2620 |
NN |
denotes |
chain |
T9438 |
2626-2629 |
NN |
denotes |
kDa |
T9439 |
2620-2622 |
, |
denotes |
, |
T9440 |
2622-2623 |
SYM |
denotes |
~ |
T9441 |
2623-2625 |
CD |
denotes |
31 |
T9442 |
2629-2630 |
-RRB- |
denotes |
) |
T9443 |
2630-2631 |
. |
denotes |
. |
T9444 |
2631-2848 |
sentence |
denotes |
Conversely, homozygous Cln3Δex7/8 and CbCln3Δex7/8 mutant (m) extracts exhibited reduced levels of precursor and heavy chain of the double-chain form of the enzyme, with elevated levels of single-chain mature enzyme. |
T9445 |
2632-2642 |
RB |
denotes |
Conversely |
T9446 |
2703-2712 |
VBD |
denotes |
exhibited |
T9447 |
2642-2644 |
, |
denotes |
, |
T9448 |
2644-2654 |
JJ |
denotes |
homozygous |
T9449 |
2694-2702 |
NNS |
denotes |
extracts |
T9450 |
2655-2663 |
NN |
denotes |
Cln3Δex7 |
T9451 |
2663-2664 |
HYPH |
denotes |
/ |
T9452 |
2664-2665 |
CD |
denotes |
8 |
T9453 |
2666-2669 |
CC |
denotes |
and |
T9454 |
2670-2680 |
NN |
denotes |
CbCln3Δex7 |
T9455 |
2680-2681 |
HYPH |
denotes |
/ |
T9456 |
2681-2682 |
CD |
denotes |
8 |
T9457 |
2683-2689 |
NN |
denotes |
mutant |
T9458 |
2690-2691 |
-LRB- |
denotes |
( |
T9459 |
2691-2692 |
NN |
denotes |
m |
T9460 |
2692-2693 |
-RRB- |
denotes |
) |
T9461 |
2713-2720 |
VBN |
denotes |
reduced |
T9462 |
2721-2727 |
NNS |
denotes |
levels |
T9463 |
2728-2730 |
IN |
denotes |
of |
T9464 |
2731-2740 |
NN |
denotes |
precursor |
T9465 |
2741-2744 |
CC |
denotes |
and |
T9466 |
2745-2750 |
JJ |
denotes |
heavy |
T9467 |
2751-2756 |
NN |
denotes |
chain |
T9468 |
2757-2759 |
IN |
denotes |
of |
T9469 |
2760-2763 |
DT |
denotes |
the |
T9470 |
2777-2781 |
NN |
denotes |
form |
T9471 |
2764-2770 |
JJ |
denotes |
double |
T9472 |
2771-2776 |
NN |
denotes |
chain |
T9473 |
2770-2771 |
HYPH |
denotes |
- |
T9474 |
2782-2784 |
IN |
denotes |
of |
T9475 |
2785-2788 |
DT |
denotes |
the |
T9476 |
2789-2795 |
NN |
denotes |
enzyme |
T9477 |
2795-2797 |
, |
denotes |
, |
T9478 |
2797-2801 |
IN |
denotes |
with |
T9479 |
2802-2810 |
JJ |
denotes |
elevated |
T9480 |
2811-2817 |
NNS |
denotes |
levels |
T9481 |
2818-2820 |
IN |
denotes |
of |
T9482 |
2821-2827 |
JJ |
denotes |
single |
T9483 |
2828-2833 |
NN |
denotes |
chain |
T9484 |
2827-2828 |
HYPH |
denotes |
- |
T9485 |
2841-2847 |
NN |
denotes |
enzyme |
T9486 |
2834-2840 |
JJ |
denotes |
mature |
T9487 |
2847-2848 |
. |
denotes |
. |
T2791 |
1421-1425 |
JJ |
denotes |
wild |
T2792 |
1426-1430 |
NN |
denotes |
type |
T2793 |
1425-1426 |
HYPH |
denotes |
- |
T2794 |
1431-1439 |
NNS |
denotes |
extracts |
T2795 |
1439-1440 |
. |
denotes |
. |
T2796 |
1440-1559 |
sentence |
denotes |
The cellular growth media did not contain altered levels of cathepsin D, indicating enzyme secretion was not affected. |
T2797 |
1441-1444 |
DT |
denotes |
The |
T2798 |
1461-1466 |
NNS |
denotes |
media |
T2799 |
1445-1453 |
JJ |
denotes |
cellular |
T2800 |
1454-1460 |
NN |
denotes |
growth |
T2801 |
1475-1482 |
VB |
denotes |
contain |
T2802 |
1467-1470 |
VBD |
denotes |
did |
T2803 |
1471-1474 |
RB |
denotes |
not |
T2804 |
1483-1490 |
VBN |
denotes |
altered |
T2805 |
1491-1497 |
NNS |
denotes |
levels |
T2806 |
1498-1500 |
IN |
denotes |
of |
T2807 |
1501-1510 |
NN |
denotes |
cathepsin |
T2808 |
1511-1512 |
NN |
denotes |
D |
T2809 |
1512-1514 |
, |
denotes |
, |
T2810 |
1514-1524 |
VBG |
denotes |
indicating |
T2811 |
1525-1531 |
NN |
denotes |
enzyme |
T2812 |
1532-1541 |
NN |
denotes |
secretion |
T2813 |
1550-1558 |
VBN |
denotes |
affected |
T2814 |
1542-1545 |
VBD |
denotes |
was |
T2815 |
1546-1549 |
RB |
denotes |
not |
T2816 |
1558-1559 |
. |
denotes |
. |
T2817 |
1559-1714 |
sentence |
denotes |
Heterozygous Cln3Δex7/8 mice and CbCln3Δex7/8 cells were indistinguishable from wild-type, as expected for a recessive disease phenotype (data not shown). |
T2818 |
1560-1572 |
JJ |
denotes |
Heterozygous |
T2819 |
1584-1588 |
NNS |
denotes |
mice |
T2820 |
1573-1581 |
NN |
denotes |
Cln3Δex7 |
T2821 |
1581-1582 |
HYPH |
denotes |
/ |
T2822 |
1582-1583 |
CD |
denotes |
8 |
T2823 |
1612-1616 |
VBD |
denotes |
were |
T2824 |
1589-1592 |
CC |
denotes |
and |
T2825 |
1593-1603 |
NN |
denotes |
CbCln3Δex7 |
T2826 |
1606-1611 |
NNS |
denotes |
cells |
T2827 |
1603-1604 |
HYPH |
denotes |
/ |
T2828 |
1604-1605 |
CD |
denotes |
8 |
T2829 |
1617-1634 |
JJ |
denotes |
indistinguishable |
T2830 |
1635-1639 |
IN |
denotes |
from |
T2831 |
1640-1644 |
JJ |
denotes |
wild |
T2832 |
1645-1649 |
NN |
denotes |
type |
T2833 |
1644-1645 |
HYPH |
denotes |
- |
T2834 |
1649-1651 |
, |
denotes |
, |
T2835 |
1651-1653 |
IN |
denotes |
as |
T2836 |
1654-1662 |
VBN |
denotes |
expected |
T2837 |
1663-1666 |
IN |
denotes |
for |
T2849 |
2849-2868 |
sentence |
denotes |
The impact of the a |
T2850 |
2849-2852 |
DT |
denotes |
The |
T2851 |
2853-2854 |
NN |
denotes |
i |
T2852 |
2854-2855 |
JJ |
denotes |
m |
T2853 |
2855-2856 |
NN |
denotes |
p |
T2854 |
2856-2857 |
CC |
denotes |
a |
T2855 |
2857-2858 |
JJ |
denotes |
c |
T2856 |
2858-2859 |
NNS |
denotes |
t |
T2857 |
2859-2860 |
, |
denotes |
|
T2858 |
2860-2861 |
JJR |
denotes |
o |
T2859 |
2861-2862 |
IN |
denotes |
f |
T2860 |
2863-2864 |
JJ |
denotes |
t |
T2861 |
2864-2865 |
NN |
denotes |
h |
T2862 |
2865-2866 |
JJ |
denotes |
e |
T2863 |
2867-2868 |
NN |
denotes |
a |
T2864 |
2868-2880 |
sentence |
denotes |
ltered cathe |
T2865 |
2868-2869 |
RB |
denotes |
l |
T2866 |
2872-2874 |
VBD |
denotes |
ed |
T2867 |
2870-2872 |
NNS |
denotes |
er |
T2868 |
2869-2870 |
NN |
denotes |
t |
T2869 |
2875-2876 |
VBN |
denotes |
c |
T2870 |
2876-2877 |
CC |
denotes |
a |
T2871 |
2877-2880 |
DT |
denotes |
the |
T2872 |
2880-3023 |
sentence |
denotes |
psin D processing on enzymatic activity was next tested to determine if altered enzymatic activity accounts for inefficient subunit c turnover. |
T2873 |
2880-2881 |
NN |
denotes |
p |
T2874 |
2929-2935 |
VBN |
denotes |
tested |
T2875 |
2887-2897 |
NN |
denotes |
processing |
T2876 |
2881-2884 |
NN |
denotes |
sin |
T2877 |
2885-2886 |
NN |
denotes |
D |
T2878 |
2898-2900 |
IN |
denotes |
on |
T2879 |
2901-2910 |
JJ |
denotes |
enzymatic |
T2880 |
2911-2919 |
NN |
denotes |
activity |
T2881 |
2920-2923 |
VBD |
denotes |
was |
T2882 |
2924-2928 |
RB |
denotes |
next |
T2883 |
2936-2938 |
TO |
denotes |
to |
T2884 |
2939-2948 |
VB |
denotes |
determine |
T2885 |
2949-2951 |
IN |
denotes |
if |
T2886 |
2979-2987 |
VBZ |
denotes |
accounts |
T2887 |
2952-2959 |
VBN |
denotes |
altered |
T2888 |
2970-2978 |
NN |
denotes |
activity |
R5964 |
T9402 |
T9395 |
punct |
", ",band |
R5965 |
T9403 |
T9395 |
acl |
representing,band |
R5966 |
T9404 |
T9403 |
dobj |
precursor,representing |
R5967 |
T9405 |
T9401 |
punct |
", ",was |
R5968 |
T9406 |
T9407 |
det |
the,band |
R5969 |
T9407 |
T9401 |
attr |
band,was |
R5970 |
T9408 |
T9407 |
amod |
predominant,band |
R5971 |
T9409 |
T9407 |
prep |
in,band |
R5834 |
T9260 |
T9261 |
amod |
wild,type |
R5835 |
T9261 |
T9263 |
nmod |
type,cells |
R5836 |
T9262 |
T9261 |
punct |
-,type |
R5838 |
T9264 |
T9261 |
cc |
and,type |
R5839 |
T9265 |
T9261 |
conj |
homozygous,type |
R5840 |
T9266 |
T9263 |
nmod |
CbCln3Δex7,cells |
R5841 |
T9267 |
T9266 |
punct |
/,CbCln3Δex7 |
R5862 |
T9290 |
T9273 |
acl |
recognizing,Immunostaining |
R5863 |
T9291 |
T9292 |
amod |
unprocessed,forms |
R5864 |
T9292 |
T9290 |
dobj |
forms,recognizing |
R5865 |
T9293 |
T9291 |
cc |
and,unprocessed |
R5866 |
T9294 |
T9291 |
conj |
processed,unprocessed |
R5867 |
T9295 |
T9292 |
prep |
of,forms |
R5868 |
T9296 |
T9297 |
compound |
cathepsin,D |
R5869 |
T9297 |
T9298 |
compound |
D,protein |
R5870 |
T9298 |
T9295 |
pobj |
protein,of |
R5871 |
T9299 |
T9271 |
auxpass |
is,shown |
R5872 |
T9300 |
T9271 |
punct |
.,shown |
R5873 |
T9302 |
T9303 |
nmod |
CbCln3,cells |
R5874 |
T9303 |
T9307 |
nsubj |
cells,exhibited |
R5875 |
T9304 |
T9302 |
punct |
+,CbCln3 |
R5876 |
T9305 |
T9302 |
punct |
/,CbCln3 |
R5877 |
T9306 |
T9302 |
punct |
+,CbCln3 |
R5878 |
T9308 |
T9309 |
punct |
(,panel |
R5879 |
T9309 |
T9303 |
parataxis |
panel,cells |
R5880 |
T9310 |
T9309 |
amod |
left,panel |
R5881 |
T9311 |
T9309 |
punct |
),panel |
R5882 |
T9312 |
T9313 |
det |
a,signal |
R5883 |
T9313 |
T9307 |
dobj |
signal,exhibited |
R5884 |
T9314 |
T9313 |
amod |
perinuclear,signal |
R5885 |
T9315 |
T9314 |
cc |
and,perinuclear |
R5886 |
T9316 |
T9314 |
conj |
cytoplasmic,perinuclear |
R5887 |
T9317 |
T9313 |
amod |
punctate,signal |
R5888 |
T9318 |
T9307 |
punct |
.,exhibited |
R5889 |
T9320 |
T9321 |
compound |
Cathepsin,D |
R5890 |
T9321 |
T9322 |
compound |
D,signal |
R5891 |
T9322 |
T9323 |
nsubj |
signal,was |
R5892 |
T9324 |
T9322 |
prep |
in,signal |
R5893 |
T9325 |
T9326 |
amod |
homozygous,cells |
R5894 |
T9326 |
T9324 |
pobj |
cells,in |
R5895 |
T9327 |
T9326 |
nmod |
CbCln3Δex7,cells |
R5896 |
T9328 |
T9327 |
punct |
/,CbCln3Δex7 |
R5897 |
T9329 |
T9327 |
nummod |
8,CbCln3Δex7 |
R5898 |
T9330 |
T9331 |
punct |
(,panel |
R5899 |
T9331 |
T9322 |
parataxis |
panel,signal |
R5900 |
T9332 |
T9331 |
amod |
right,panel |
R5901 |
T9333 |
T9331 |
punct |
),panel |
R5902 |
T9334 |
T9335 |
advmod |
more,often |
R5903 |
T9335 |
T9323 |
advmod |
often,was |
R5904 |
T9336 |
T9323 |
acomp |
perinuclear,was |
R5905 |
T9337 |
T9323 |
punct |
", ",was |
R5906 |
T9338 |
T9323 |
prep |
with,was |
R5907 |
T9339 |
T9340 |
advmod |
less,signal |
R5908 |
T9340 |
T9338 |
pobj |
signal,with |
R5909 |
T9341 |
T9342 |
amod |
cytoplasmic,punctate |
R5910 |
T9342 |
T9340 |
compound |
punctate,signal |
R5911 |
T9343 |
T9340 |
punct |
", ",signal |
R5912 |
T9344 |
T9340 |
prep |
compared,signal |
R5913 |
T9345 |
T9344 |
prep |
to,compared |
R5914 |
T9346 |
T9347 |
amod |
wild,type |
R5915 |
T9347 |
T9349 |
nmod |
type,cells |
R5916 |
T9348 |
T9347 |
punct |
-,type |
R5917 |
T9349 |
T9345 |
pobj |
cells,to |
R5918 |
T9350 |
T9349 |
nmod |
CbCln3,cells |
R5919 |
T9351 |
T9350 |
punct |
+,CbCln3 |
R5920 |
T9352 |
T9350 |
punct |
/,CbCln3 |
R5921 |
T9353 |
T9350 |
punct |
+,CbCln3 |
R5922 |
T9354 |
T9323 |
punct |
.,was |
R5923 |
T9356 |
T9357 |
nummod |
40,magnification |
R5924 |
T9358 |
T9356 |
punct |
×,40 |
R5925 |
T9359 |
T9357 |
punct |
.,magnification |
R5926 |
T9361 |
T9362 |
meta |
b,shown |
R5927 |
T9363 |
T9361 |
punct |
.,b |
R5928 |
T9364 |
T9365 |
compound |
α,D |
R5929 |
T9365 |
T9368 |
npadvmod |
D,probed |
R5930 |
T9366 |
T9365 |
punct |
-,D |
R5931 |
T9367 |
T9365 |
compound |
Cathepsin,D |
R5932 |
T9368 |
T9370 |
amod |
probed,immunoblots |
R5933 |
T9369 |
T9368 |
punct |
-,probed |
R5934 |
T9370 |
T9362 |
nsubjpass |
immunoblots,shown |
R5935 |
T9371 |
T9370 |
prep |
of,immunoblots |
R5936 |
T9372 |
T9373 |
amod |
total,tissue |
R5938 |
T9374 |
T9375 |
amod |
wild,type |
R5939 |
T9375 |
T9373 |
nmod |
type,tissue |
R5940 |
T9376 |
T9375 |
punct |
-,type |
R5959 |
T9396 |
T9397 |
punct |
~,45 |
R5960 |
T9397 |
T9398 |
nummod |
45,kDa |
R5961 |
T9398 |
T9395 |
compound |
kDa,band |
R5962 |
T9399 |
T9400 |
compound |
cathepsin,D |
R5963 |
T9400 |
T9395 |
compound |
D,band |
R5972 |
T9410 |
T9411 |
amod |
wild,type |
R5973 |
T9411 |
T9409 |
pobj |
type,in |
R5974 |
T9412 |
T9411 |
punct |
-,type |
R5975 |
T9413 |
T9411 |
punct |
(,type |
R5976 |
T9414 |
T9411 |
appos |
wt,type |
R5977 |
T9415 |
T9411 |
punct |
),type |
R5978 |
T9416 |
T9411 |
appos |
tissue,type |
R5979 |
T9417 |
T9416 |
cc |
and,tissue |
R5980 |
T9418 |
T9419 |
amod |
cellular,extracts |
R5981 |
T9419 |
T9416 |
conj |
extracts,tissue |
R5982 |
T9420 |
T9407 |
punct |
", ",band |
R5983 |
T9421 |
T9407 |
prep |
with,band |
R5984 |
T9422 |
T9423 |
amod |
lower,levels |
R5985 |
T9423 |
T9421 |
pobj |
levels,with |
R5986 |
T9424 |
T9423 |
prep |
of,levels |
R5987 |
T9425 |
T9426 |
amod |
mature,enzyme |
R5988 |
T9426 |
T9424 |
pobj |
enzyme,of |
R5989 |
T9427 |
T9428 |
punct |
(,kDa |
R5990 |
T9428 |
T9401 |
parataxis |
kDa,was |
R5991 |
T9429 |
T9430 |
amod |
single,chain |
R5992 |
T9430 |
T9428 |
dep |
chain,kDa |
R5993 |
T9431 |
T9428 |
punct |
", ",kDa |
R5994 |
T9432 |
T9433 |
punct |
~,43 |
R5995 |
T9433 |
T9428 |
nummod |
43,kDa |
R5996 |
T9434 |
T9428 |
punct |
", ",kDa |
R5997 |
T9435 |
T9428 |
cc |
and,kDa |
R5998 |
T9436 |
T9437 |
amod |
heavy,chain |
R5999 |
T9437 |
T9438 |
dep |
chain,kDa |
R6000 |
T9438 |
T9428 |
conj |
kDa,kDa |
R6001 |
T9439 |
T9438 |
punct |
", ",kDa |
R6002 |
T9440 |
T9441 |
punct |
~,31 |
R6003 |
T9441 |
T9438 |
nummod |
31,kDa |
R6004 |
T9442 |
T9438 |
punct |
),kDa |
R6005 |
T9443 |
T9401 |
punct |
.,was |
R6006 |
T9445 |
T9446 |
advmod |
Conversely,exhibited |
R6007 |
T9447 |
T9446 |
punct |
", ",exhibited |
R6008 |
T9448 |
T9449 |
amod |
homozygous,extracts |
R6009 |
T9449 |
T9446 |
nsubj |
extracts,exhibited |
R6010 |
T9450 |
T9449 |
nmod |
Cln3Δex7,extracts |
R6011 |
T9451 |
T9450 |
punct |
/,Cln3Δex7 |
R6012 |
T9452 |
T9450 |
nummod |
8,Cln3Δex7 |
R6013 |
T9453 |
T9450 |
cc |
and,Cln3Δex7 |
R6014 |
T9454 |
T9450 |
conj |
CbCln3Δex7,Cln3Δex7 |
R6015 |
T9455 |
T9454 |
punct |
/,CbCln3Δex7 |
R6016 |
T9456 |
T9454 |
nummod |
8,CbCln3Δex7 |
R6017 |
T9457 |
T9449 |
nmod |
mutant,extracts |
R6018 |
T9458 |
T9457 |
punct |
(,mutant |
R6019 |
T9459 |
T9457 |
appos |
m,mutant |
R6020 |
T9460 |
T9449 |
punct |
),extracts |
R6021 |
T9461 |
T9462 |
amod |
reduced,levels |
R6022 |
T9462 |
T9446 |
dobj |
levels,exhibited |
R6023 |
T9463 |
T9462 |
prep |
of,levels |
R6024 |
T9464 |
T9463 |
pobj |
precursor,of |
R6025 |
T9465 |
T9464 |
cc |
and,precursor |
R6026 |
T9466 |
T9467 |
amod |
heavy,chain |
R6027 |
T9467 |
T9464 |
conj |
chain,precursor |
R6028 |
T9468 |
T9464 |
prep |
of,precursor |
R6029 |
T9469 |
T9470 |
det |
the,form |
R6030 |
T9470 |
T9468 |
pobj |
form,of |
R6031 |
T9471 |
T9472 |
amod |
double,chain |
R6032 |
T9472 |
T9470 |
compound |
chain,form |
R6033 |
T9473 |
T9472 |
punct |
-,chain |
R6034 |
T9474 |
T9470 |
prep |
of,form |
R6035 |
T9475 |
T9476 |
det |
the,enzyme |
R6036 |
T9476 |
T9474 |
pobj |
enzyme,of |
R6037 |
T9477 |
T9446 |
punct |
", ",exhibited |
R6038 |
T9478 |
T9446 |
prep |
with,exhibited |
R6039 |
T9479 |
T9480 |
amod |
elevated,levels |
R6040 |
T9480 |
T9478 |
pobj |
levels,with |
R6041 |
T9481 |
T9480 |
prep |
of,levels |
R6042 |
T9482 |
T9483 |
amod |
single,chain |
R6043 |
T9483 |
T9485 |
nmod |
chain,enzyme |
R6044 |
T9484 |
T9483 |
punct |
-,chain |
R6045 |
T9485 |
T9481 |
pobj |
enzyme,of |
R6046 |
T9486 |
T9485 |
amod |
mature,enzyme |
R6047 |
T9487 |
T9446 |
punct |
.,exhibited |
R1375 |
T2526 |
T2527 |
amod |
Homozygous,cells |
R1376 |
T2527 |
T2531 |
nsubj |
cells,process |
R1377 |
T2528 |
T2527 |
nmod |
CbCln3Δex7,cells |
R1378 |
T2529 |
T2528 |
punct |
/,CbCln3Δex7 |
R1379 |
T2530 |
T2528 |
nummod |
8,CbCln3Δex7 |
R1380 |
T2532 |
T2527 |
cc |
and,cells |
R1381 |
T2533 |
T2534 |
nmod |
Cln3Δex7,mice |
R1382 |
T2534 |
T2527 |
conj |
mice,cells |
R1383 |
T2535 |
T2533 |
punct |
/,Cln3Δex7 |
R1384 |
T2536 |
T2533 |
nummod |
8,Cln3Δex7 |
R1385 |
T2537 |
T2534 |
amod |
knock,mice |
R1386 |
T2538 |
T2537 |
punct |
-,knock |
R1387 |
T2539 |
T2537 |
prt |
in,knock |
R1388 |
T2540 |
T2541 |
compound |
cathepsin,D |
R1389 |
T2541 |
T2531 |
dobj |
D,process |
R1390 |
T2542 |
T2531 |
advmod |
abnormally,process |
R1391 |
T2544 |
T2545 |
nsubj |
We,investigated |
R1392 |
T2546 |
T2545 |
advmod |
next,investigated |
R1393 |
T2547 |
T2548 |
det |
the,basis |
R1394 |
T2548 |
T2545 |
dobj |
basis,investigated |
R1395 |
T2549 |
T2548 |
prep |
for,basis |
R1396 |
T2550 |
T2551 |
compound |
subunit,c |
R1397 |
T2551 |
T2552 |
compound |
c,accumulation |
R1398 |
T2552 |
T2549 |
pobj |
accumulation,for |
R1399 |
T2553 |
T2545 |
punct |
", ",investigated |
R1400 |
T2554 |
T2545 |
advcl |
testing,investigated |
R1401 |
T2555 |
T2556 |
det |
the,hypothesis |
R1402 |
T2556 |
T2554 |
dobj |
hypothesis,testing |
R1403 |
T2557 |
T2558 |
mark |
that,is |
R1404 |
T2558 |
T2556 |
acl |
is,hypothesis |
R1405 |
T2559 |
T2560 |
compound |
cathepsin,D |
R1406 |
T2560 |
T2558 |
nsubj |
D,is |
R1407 |
T2561 |
T2558 |
acomp |
abnormal,is |
R1408 |
T2562 |
T2563 |
mark |
since,is |
R1409 |
T2563 |
T2558 |
advcl |
is,is |
R1410 |
T2564 |
T2563 |
nsubj |
it,is |
R1411 |
T2565 |
T2563 |
acomp |
required,is |
R1412 |
T2566 |
T2565 |
prep |
for,required |
R1413 |
T2567 |
T2568 |
compound |
ATP,degradation |
R1414 |
T2568 |
T2566 |
pobj |
degradation,for |
R1415 |
T2569 |
T2568 |
compound |
synthase,degradation |
R1416 |
T2570 |
T2571 |
compound |
subunit,c |
R1417 |
T2571 |
T2568 |
compound |
c,degradation |
R1418 |
T2572 |
T2568 |
prep |
in,degradation |
R1419 |
T2573 |
T2574 |
det |
the,lysosome |
R1420 |
T2574 |
T2572 |
pobj |
lysosome,in |
R1421 |
T2575 |
T2576 |
punct |
[,13 |
R1422 |
T2576 |
T2554 |
parataxis |
13,testing |
R1423 |
T2577 |
T2576 |
punct |
],13 |
R1424 |
T2578 |
T2545 |
punct |
.,investigated |
R1425 |
T2580 |
T2581 |
nsubj |
We,tested |
R1426 |
T2582 |
T2581 |
advmod |
first,tested |
R1427 |
T2583 |
T2584 |
compound |
cathepsin,D |
R1428 |
T2584 |
T2581 |
dobj |
D,tested |
R1429 |
T2585 |
T2584 |
appos |
transport,D |
R1430 |
T2586 |
T2585 |
cc |
and,transport |
R1431 |
T2587 |
T2585 |
conj |
processing,transport |
R1432 |
T2588 |
T2581 |
prep |
in,tested |
R1433 |
T2589 |
T2590 |
amod |
homozygous,cells |
R1434 |
T2590 |
T2588 |
pobj |
cells,in |
R1435 |
T2591 |
T2590 |
nmod |
CbCln3Δex7,cells |
R1436 |
T2592 |
T2591 |
punct |
/,CbCln3Δex7 |
R1437 |
T2593 |
T2591 |
nummod |
8,CbCln3Δex7 |
R1438 |
T2594 |
T2590 |
cc |
and,cells |
R1439 |
T2595 |
T2596 |
nmod |
Cln3Δex7,mice |
R1440 |
T2596 |
T2590 |
conj |
mice,cells |
R1441 |
T2597 |
T2595 |
punct |
/,Cln3Δex7 |
R1442 |
T2598 |
T2595 |
nummod |
8,Cln3Δex7 |
R1443 |
T2599 |
T2581 |
advcl |
using,tested |
R1444 |
T2600 |
T2601 |
amod |
anti-cathepsin,D |
R1445 |
T2601 |
T2602 |
amod |
D,antibody |
R1446 |
T2602 |
T2599 |
dobj |
antibody,using |
R1447 |
T2603 |
T2604 |
dep |
that,recognizes |
R1448 |
T2604 |
T2602 |
relcl |
recognizes,antibody |
R1449 |
T2605 |
T2606 |
amod |
unprocessed,isoforms |
R1450 |
T2606 |
T2604 |
dobj |
isoforms,recognizes |
R1451 |
T2607 |
T2605 |
cc |
and,unprocessed |
R1452 |
T2608 |
T2605 |
conj |
processed,unprocessed |
R1453 |
T2609 |
T2610 |
compound |
cathepsin,D |
R1454 |
T2610 |
T2606 |
compound |
D,isoforms |
R1455 |
T2611 |
T2581 |
punct |
.,tested |
R1456 |
T2613 |
T2614 |
nsubj |
Immunostaining,revealed |
R1457 |
T2615 |
T2613 |
prep |
of,Immunostaining |
R1458 |
T2616 |
T2617 |
amod |
wild,type |
R1459 |
T2617 |
T2619 |
nmod |
type,cells |
R1460 |
T2618 |
T2617 |
punct |
-,type |
R1461 |
T2619 |
T2615 |
pobj |
cells,of |
R1462 |
T2620 |
T2617 |
cc |
and,type |
R1463 |
T2621 |
T2617 |
conj |
homozygous,type |
R1464 |
T2622 |
T2619 |
nmod |
CbCln3Δex7,cells |
R1465 |
T2623 |
T2622 |
punct |
/,CbCln3Δex7 |
R1466 |
T2624 |
T2622 |
nummod |
8,CbCln3Δex7 |
R1467 |
T2625 |
T2626 |
det |
a,distribution |
R1468 |
T2626 |
T2614 |
dobj |
distribution,revealed |
R1469 |
T2627 |
T2626 |
amod |
perinuclear,distribution |
R1470 |
T2628 |
T2627 |
cc |
and,perinuclear |
R1471 |
T2629 |
T2627 |
conj |
punctate,perinuclear |
R1472 |
T2630 |
T2626 |
amod |
vesicular,distribution |
R1473 |
T2631 |
T2632 |
compound |
cathepsin,D |
R1474 |
T2632 |
T2626 |
compound |
D,distribution |
R1475 |
T2633 |
T2614 |
punct |
", ",revealed |
R1476 |
T2634 |
T2614 |
advcl |
consistent,revealed |
R1477 |
T2635 |
T2634 |
prep |
with,consistent |
R1478 |
T2636 |
T2637 |
poss |
its,transport |
R1479 |
T2637 |
T2635 |
pobj |
transport,with |
R1480 |
T2638 |
T2637 |
cc |
and,transport |
R1481 |
T2639 |
T2637 |
conj |
processing,transport |
R1482 |
T2640 |
T2637 |
prep |
through,transport |
R1483 |
T2641 |
T2642 |
det |
the,pathway |
R1484 |
T2642 |
T2640 |
pobj |
pathway,through |
R1485 |
T2643 |
T2642 |
amod |
secretory,pathway |
R1486 |
T2644 |
T2637 |
cc |
and,transport |
R1487 |
T2645 |
T2637 |
conj |
delivery,transport |
R1488 |
T2646 |
T2645 |
prep |
to,delivery |
R1489 |
T2647 |
T2648 |
det |
the,lysosome |
R1490 |
T2648 |
T2646 |
pobj |
lysosome,to |
R1491 |
T2649 |
T2650 |
punct |
(,5a |
R1492 |
T2650 |
T2614 |
parataxis |
5a,revealed |
R1493 |
T2651 |
T2650 |
compound |
Fig.,5a |
R1494 |
T2652 |
T2650 |
punct |
),5a |
R1495 |
T2653 |
T2614 |
punct |
.,revealed |
R1496 |
T2655 |
T2656 |
advmod |
However,was |
R1497 |
T2657 |
T2656 |
punct |
", ",was |
R1498 |
T2658 |
T2656 |
prep |
in,was |
R1499 |
T2659 |
T2660 |
amod |
homozygous,cells |
R1500 |
T2660 |
T2658 |
pobj |
cells,in |
R1501 |
T2661 |
T2660 |
nmod |
CbCln3Δex7,cells |
R1502 |
T2662 |
T2661 |
punct |
/,CbCln3Δex7 |
R1503 |
T2663 |
T2661 |
nummod |
8,CbCln3Δex7 |
R1504 |
T2664 |
T2656 |
punct |
", ",was |
R1505 |
T2665 |
T2666 |
compound |
cathepsin,D |
R1506 |
T2666 |
T2667 |
compound |
D,distribution |
R1507 |
T2667 |
T2656 |
nsubj |
distribution,was |
R1508 |
T2668 |
T2669 |
advmod |
less,vesicular |
R1509 |
T2669 |
T2656 |
acomp |
vesicular,was |
R1510 |
T2670 |
T2669 |
cc |
and,vesicular |
R1511 |
T2671 |
T2672 |
advmod |
more,clustered |
R1512 |
T2672 |
T2669 |
conj |
clustered,vesicular |
R1513 |
T2673 |
T2672 |
amod |
perinuclear,clustered |
R1514 |
T2674 |
T2672 |
punct |
-,clustered |
R1515 |
T2675 |
T2669 |
prep |
than,vesicular |
R1516 |
T2676 |
T2675 |
prep |
in,than |
R1517 |
T2677 |
T2678 |
amod |
wild,type |
R1518 |
T2678 |
T2680 |
compound |
type,cells |
R1519 |
T2679 |
T2678 |
punct |
-,type |
R1520 |
T2680 |
T2676 |
pobj |
cells,in |
R1521 |
T2681 |
T2656 |
punct |
.,was |
R1522 |
T2683 |
T2684 |
nsubj |
Immunoblots,showed |
R1523 |
T2685 |
T2683 |
prep |
of,Immunoblots |
R1524 |
T2686 |
T2687 |
amod |
homozygous,extracts |
R1525 |
T2687 |
T2685 |
pobj |
extracts,of |
R1526 |
T2688 |
T2689 |
nmod |
CbCln3Δex7,cell |
R1527 |
T2689 |
T2687 |
nmod |
cell,extracts |
R1528 |
T2690 |
T2688 |
punct |
/,CbCln3Δex7 |
R1529 |
T2691 |
T2688 |
nummod |
8,CbCln3Δex7 |
R1530 |
T2692 |
T2689 |
cc |
and,cell |
R1531 |
T2693 |
T2694 |
nmod |
Cln3Δex7,tissue |
R1532 |
T2694 |
T2689 |
conj |
tissue,cell |
R1533 |
T2695 |
T2693 |
punct |
/,Cln3Δex7 |
R1534 |
T2696 |
T2693 |
nummod |
8,Cln3Δex7 |
R1535 |
T2697 |
T2684 |
advmod |
also,showed |
R1536 |
T2698 |
T2699 |
amod |
altered,levels |
R1537 |
T2699 |
T2684 |
dobj |
levels,showed |
R1538 |
T2700 |
T2699 |
amod |
relative,levels |
R1539 |
T2701 |
T2699 |
prep |
of,levels |
R1540 |
T2702 |
T2703 |
compound |
cathepsin,D |
R1541 |
T2703 |
T2704 |
compound |
D,isoforms |
R1542 |
T2704 |
T2701 |
pobj |
isoforms,of |
R1543 |
T2705 |
T2706 |
punct |
(,5b |
R1544 |
T2706 |
T2684 |
parataxis |
5b,showed |
R1545 |
T2707 |
T2706 |
compound |
Fig.,5b |
R1546 |
T2708 |
T2706 |
punct |
),5b |
R1547 |
T2709 |
T2684 |
punct |
.,showed |
R1548 |
T2711 |
T2712 |
compound |
Cathepsin,D |
R1549 |
T2712 |
T2713 |
compound |
D,isoforms |
R1550 |
T2713 |
T2714 |
nsubj |
isoforms,represent |
R1551 |
T2715 |
T2713 |
punct |
", ",isoforms |
R1552 |
T2716 |
T2713 |
acl |
identified,isoforms |
R1553 |
T2717 |
T2716 |
agent |
by,identified |
R1554 |
T2718 |
T2719 |
amod |
relative,weights |
R1555 |
T2719 |
T2717 |
pobj |
weights,by |
R1556 |
T2720 |
T2719 |
amod |
molecular,weights |
R1557 |
T2721 |
T2714 |
punct |
", ",represent |
R1558 |
T2722 |
T2723 |
det |
the,precursor |
R1559 |
T2723 |
T2714 |
dobj |
precursor,represent |
R1560 |
T2724 |
T2725 |
punct |
~,45 |
R1561 |
T2725 |
T2726 |
nummod |
45,kDa |
R1562 |
T2726 |
T2723 |
compound |
kDa,precursor |
R1563 |
T2727 |
T2723 |
punct |
", ",precursor |
R1564 |
T2728 |
T2729 |
det |
the,form |
R1565 |
T2729 |
T2723 |
conj |
form,precursor |
R1566 |
T2730 |
T2731 |
punct |
~,43 |
R1567 |
T2731 |
T2732 |
nummod |
43,kDa |
R1568 |
T2732 |
T2729 |
nmod |
kDa,form |
R1569 |
T2733 |
T2729 |
amod |
intermediate,form |
R1570 |
T2734 |
T2735 |
amod |
single,chain |
R1571 |
T2735 |
T2729 |
compound |
chain,form |
R1572 |
T2736 |
T2729 |
prep |
of,form |
R1573 |
T2737 |
T2738 |
det |
the,enzyme |
R1574 |
T2738 |
T2736 |
pobj |
enzyme,of |
R1575 |
T2739 |
T2729 |
punct |
", ",form |
R1576 |
T2740 |
T2729 |
cc |
and,form |
R1577 |
T2741 |
T2742 |
det |
the,chain |
R1578 |
T2742 |
T2729 |
conj |
chain,form |
R1579 |
T2743 |
T2744 |
nummod |
31,kDa |
R1580 |
T2744 |
T2742 |
nmod |
kDa,chain |
R1581 |
T2745 |
T2742 |
amod |
heavy,chain |
R1582 |
T2746 |
T2742 |
prep |
of,chain |
R1583 |
T2747 |
T2748 |
det |
the,form |
R1584 |
T2748 |
T2746 |
pobj |
form,of |
R1585 |
T2749 |
T2750 |
amod |
double,chain |
R1586 |
T2750 |
T2748 |
compound |
chain,form |
R1587 |
T2751 |
T2750 |
punct |
-,chain |
R1588 |
T2752 |
T2748 |
prep |
of,form |
R1589 |
T2753 |
T2754 |
det |
the,enzyme |
R1590 |
T2754 |
T2752 |
pobj |
enzyme,of |
R1591 |
T2755 |
T2754 |
amod |
mature,enzyme |
R1592 |
T2756 |
T2757 |
punct |
[,14 |
R1593 |
T2757 |
T2714 |
parataxis |
14,represent |
R1594 |
T2758 |
T2757 |
punct |
],14 |
R1595 |
T2759 |
T2714 |
punct |
.,represent |
R1596 |
T2761 |
T2762 |
prep |
In,reduced |
R1597 |
T2763 |
T2764 |
amod |
homozygous,extracts |
R1598 |
T2764 |
T2761 |
pobj |
extracts,In |
R1599 |
T2765 |
T2766 |
nmod |
CbCln3Δex7,cell |
R1600 |
T2766 |
T2764 |
nmod |
cell,extracts |
R1601 |
T2767 |
T2765 |
punct |
/,CbCln3Δex7 |
R1602 |
T2768 |
T2765 |
nummod |
8,CbCln3Δex7 |
R1603 |
T2769 |
T2766 |
cc |
and,cell |
R1604 |
T2770 |
T2771 |
nmod |
Cln3Δex7,tissue |
R1605 |
T2771 |
T2766 |
conj |
tissue,cell |
R1606 |
T2772 |
T2770 |
punct |
/,Cln3Δex7 |
R1607 |
T2773 |
T2770 |
nummod |
8,Cln3Δex7 |
R1608 |
T2774 |
T2762 |
punct |
", ",reduced |
R1609 |
T2775 |
T2776 |
det |
the,precursor |
R1610 |
T2776 |
T2762 |
nsubjpass |
precursor,reduced |
R1611 |
T2777 |
T2776 |
cc |
and,precursor |
R1612 |
T2778 |
T2779 |
amod |
heavy,chains |
R1613 |
T2779 |
T2776 |
conj |
chains,precursor |
R1614 |
T2780 |
T2762 |
auxpass |
were,reduced |
R1615 |
T2781 |
T2762 |
punct |
", ",reduced |
R1616 |
T2782 |
T2762 |
cc |
and,reduced |
R1617 |
T2783 |
T2784 |
det |
the,chain |
R1618 |
T2784 |
T2786 |
nsubj |
chain,was |
R1619 |
T2785 |
T2784 |
amod |
single,chain |
R1620 |
T2786 |
T2762 |
conj |
was,reduced |
R1621 |
T2787 |
T2788 |
advmod |
slightly,elevated |
R1622 |
T2788 |
T2786 |
acomp |
elevated,was |
R1623 |
T2789 |
T2786 |
prep |
compared,was |
R1624 |
T2790 |
T2789 |
prep |
to,compared |
R1625 |
T2791 |
T2792 |
amod |
wild,type |
R1626 |
T2792 |
T2794 |
compound |
type,extracts |
R1627 |
T2793 |
T2792 |
punct |
-,type |
R1628 |
T2794 |
T2790 |
pobj |
extracts,to |
R1629 |
T2795 |
T2786 |
punct |
.,was |
R1630 |
T2797 |
T2798 |
det |
The,media |
R1631 |
T2798 |
T2801 |
nsubj |
media,contain |
R1632 |
T2799 |
T2800 |
amod |
cellular,growth |
R1633 |
T2800 |
T2798 |
compound |
growth,media |
R1634 |
T2802 |
T2801 |
aux |
did,contain |
R1635 |
T2803 |
T2801 |
neg |
not,contain |
R1636 |
T2804 |
T2805 |
amod |
altered,levels |
R1637 |
T2805 |
T2801 |
dobj |
levels,contain |
R1638 |
T2806 |
T2805 |
prep |
of,levels |
R1639 |
T2807 |
T2808 |
compound |
cathepsin,D |
R1640 |
T2808 |
T2806 |
pobj |
D,of |
R1641 |
T2809 |
T2801 |
punct |
", ",contain |
R1642 |
T2810 |
T2801 |
advcl |
indicating,contain |
R1700 |
T2892 |
T2890 |
pobj |
turnover,for |
R1701 |
T2893 |
T2894 |
compound |
subunit,c |
R1702 |
T2894 |
T2892 |
compound |
c,turnover |
R1703 |
T2895 |
T2874 |
punct |
.,tested |
R1704 |
T2897 |
T2898 |
prep |
In,altered |
R1705 |
T2899 |
T2900 |
det |
a,assay |
R1706 |
T2900 |
T2897 |
pobj |
assay,In |
R1707 |
T2901 |
T2900 |
amod |
fluorogenic,assay |
R1708 |
T2902 |
T2903 |
advmod |
in,vitro |
R1709 |
T2903 |
T2900 |
amod |
vitro,assay |
R1710 |
T2904 |
T2898 |
punct |
", ",altered |
R1711 |
T2905 |
T2906 |
compound |
cathepsin,D |
R1712 |
T2906 |
T2907 |
compound |
D,activity |
R1713 |
T2907 |
T2898 |
nsubjpass |
activity,altered |
R1714 |
T2908 |
T2907 |
prep |
in,activity |
R1715 |
T2909 |
T2910 |
amod |
total,extracts |
R1716 |
T2910 |
T2908 |
pobj |
extracts,in |
R1717 |
T2911 |
T2910 |
amod |
cellular,extracts |
R1718 |
T2912 |
T2898 |
auxpass |
was,altered |
R1719 |
T2913 |
T2898 |
neg |
not,altered |
R1720 |
T2914 |
T2898 |
advmod |
significantly,altered |
R1721 |
T2915 |
T2898 |
prep |
in,altered |
R1722 |
T2916 |
T2917 |
amod |
homozygous,cells |
R1723 |
T2917 |
T2915 |
pobj |
cells,in |
R1724 |
T2918 |
T2917 |
nmod |
CbCln3Δex7,cells |
R1725 |
T2919 |
T2918 |
punct |
/,CbCln3Δex7 |
R1726 |
T2920 |
T2918 |
nummod |
8,CbCln3Δex7 |
R1727 |
T2921 |
T2922 |
punct |
(,RFU |
R1728 |
T2922 |
T2917 |
parataxis |
RFU,cells |
R1729 |
T2923 |
T2924 |
quantmod |
376,89 |
R1730 |
T2924 |
T2922 |
nummod |
89,RFU |
R1731 |
T2925 |
T2924 |
punct |
±,89 |
R1732 |
T2926 |
T2927 |
punct |
/,protein |
R1733 |
T2927 |
T2922 |
prep |
protein,RFU |
R1734 |
T2928 |
T2927 |
nmod |
μg,protein |
R1735 |
T2929 |
T2927 |
amod |
total,protein |
R1736 |
T2930 |
T2922 |
punct |
),RFU |
R1737 |
T2931 |
T2917 |
punct |
", ",cells |
R1738 |
T2932 |
T2917 |
cc |
versus,cells |
R1739 |
T2933 |
T2934 |
amod |
wild,type |
R1740 |
T2934 |
T2936 |
compound |
type,cells |
R1741 |
T2935 |
T2934 |
punct |
-,type |
R1742 |
T2936 |
T2917 |
conj |
cells,cells |
R1743 |
T2937 |
T2938 |
punct |
(,RFU |
R1744 |
T2938 |
T2936 |
parataxis |
RFU,cells |
R1745 |
T2939 |
T2940 |
quantmod |
324,58 |
R1746 |
T2940 |
T2938 |
nummod |
58,RFU |
R1747 |
T2941 |
T2940 |
quantmod |
±,58 |
R1748 |
T2942 |
T2943 |
punct |
/,protein |
R1749 |
T2943 |
T2938 |
prep |
protein,RFU |
R1750 |
T2944 |
T2943 |
nmod |
μg,protein |
R1751 |
T2945 |
T2943 |
amod |
total,protein |
R1752 |
T2946 |
T2938 |
punct |
),RFU |
R1753 |
T2947 |
T2898 |
punct |
", ",altered |
R1754 |
T2948 |
T2949 |
mark |
although,observed |
R1755 |
T2949 |
T2898 |
advcl |
observed,altered |
R1756 |
T2950 |
T2951 |
det |
a,trend |
R1757 |
T2951 |
T2949 |
nsubjpass |
trend,observed |
R1758 |
T2952 |
T2951 |
amod |
consistent,trend |
R1759 |
T2953 |
T2951 |
prep |
towards,trend |
R1760 |
T2954 |
T2955 |
amod |
increased,activity |
R1761 |
T2955 |
T2953 |
pobj |
activity,towards |
R1762 |
T2956 |
T2955 |
amod |
enzymatic,activity |
R1763 |
T2957 |
T2955 |
prep |
in,activity |
R1764 |
T2958 |
T2959 |
compound |
mutant,cells |
R1765 |
T2959 |
T2957 |
pobj |
cells,in |
R1766 |
T2960 |
T2949 |
auxpass |
was,observed |
R1767 |
T2961 |
T2898 |
punct |
.,altered |
R1768 |
T2963 |
T2964 |
advmod |
Thus,disrupted |
R1769 |
T2965 |
T2964 |
punct |
", ",disrupted |
R1770 |
T2966 |
T2967 |
compound |
cathepsin,D |
R1771 |
T2967 |
T2964 |
nsubjpass |
D,disrupted |
R1772 |
T2968 |
T2967 |
appos |
transport,D |
R1773 |
T2969 |
T2968 |
cc |
and,transport |
R1774 |
T2970 |
T2968 |
conj |
processing,transport |
R1775 |
T2971 |
T2964 |
auxpass |
are,disrupted |
R1776 |
T2972 |
T2964 |
prep |
in,disrupted |
R1777 |
T2973 |
T2974 |
amod |
homozygous,cells |
R1778 |
T2974 |
T2972 |
pobj |
cells,in |
R1779 |
T2975 |
T2974 |
nmod |
CbCln3Δex7,cells |
R1780 |
T2976 |
T2975 |
punct |
/,CbCln3Δex7 |
R1781 |
T2977 |
T2975 |
nummod |
8,CbCln3Δex7 |
R1782 |
T2978 |
T2964 |
prep |
in,disrupted |
R1783 |
T2979 |
T2980 |
det |
a,manner |
R1784 |
T2980 |
T2978 |
pobj |
manner,in |
R1785 |
T2981 |
T2980 |
amod |
such,manner |
R1786 |
T2982 |
T2983 |
mark |
that,appears |
R1787 |
T2983 |
T2981 |
ccomp |
appears,such |
R1788 |
T2984 |
T2985 |
amod |
enzymatic,activity |
R1789 |
T2985 |
T2983 |
nsubj |
activity,appears |
R1790 |
T2986 |
T2987 |
aux |
to,be |
R1791 |
T2987 |
T2983 |
xcomp |
be,appears |
R1792 |
T2988 |
T2989 |
advmod |
relatively,unaffected |
R1793 |
T2989 |
T2987 |
acomp |
unaffected,be |
R1794 |
T2990 |
T2964 |
punct |
.,disrupted |
R5844 |
T9272 |
T9270 |
punct |
.,a |
R1687 |
T2879 |
T2880 |
amod |
enzymatic,activity |
R1688 |
T2880 |
T2878 |
pobj |
activity,on |
R1689 |
T2881 |
T2874 |
auxpass |
was,tested |
R1690 |
T2882 |
T2874 |
advmod |
next,tested |
R1691 |
T2883 |
T2884 |
aux |
to,determine |
R1692 |
T2884 |
T2874 |
advcl |
determine,tested |
R1693 |
T2885 |
T2886 |
mark |
if,accounts |
R1694 |
T2886 |
T2884 |
ccomp |
accounts,determine |
R1695 |
T2887 |
T2888 |
amod |
altered,activity |
R1696 |
T2888 |
T2886 |
nsubj |
activity,accounts |
R1697 |
T2889 |
T2888 |
amod |
enzymatic,activity |
R1698 |
T2890 |
T2886 |
prep |
for,accounts |
R1699 |
T2891 |
T2892 |
amod |
inefficient,turnover |
R5829 |
T9254 |
T9255 |
compound |
Cathepsin,D |
R5830 |
T9256 |
T9255 |
appos |
localization,D |
R5831 |
T9257 |
T9256 |
cc |
and,localization |
R5832 |
T9258 |
T9256 |
conj |
processing,localization |
R5833 |
T9259 |
T9255 |
prep |
in,D |
R5837 |
T9263 |
T9259 |
pobj |
cells,in |
R5842 |
T9268 |
T9266 |
nummod |
8,CbCln3Δex7 |
R5843 |
T9270 |
T9271 |
meta |
a,shown |
R5845 |
T9273 |
T9271 |
nsubjpass |
Immunostaining,shown |
R5846 |
T9274 |
T9273 |
prep |
of,Immunostaining |
R5847 |
T9275 |
T9276 |
amod |
wild,type |
R5848 |
T9276 |
T9278 |
nmod |
type,cells |
R5849 |
T9277 |
T9276 |
punct |
-,type |
R5850 |
T9278 |
T9274 |
pobj |
cells,of |
R5851 |
T9279 |
T9276 |
cc |
and,type |
R5852 |
T9280 |
T9276 |
conj |
homozygous,type |
R5853 |
T9281 |
T9278 |
nmod |
CbCln3Δex7,cells |
R5854 |
T9282 |
T9281 |
punct |
/,CbCln3Δex7 |
R5855 |
T9283 |
T9281 |
nummod |
8,CbCln3Δex7 |
R5856 |
T9284 |
T9278 |
compound |
precursor,cells |
R5857 |
T9285 |
T9273 |
prep |
with,Immunostaining |
R5858 |
T9286 |
T9287 |
amod |
anti-cathepsin,D |
R5859 |
T9287 |
T9288 |
amod |
D,antibody |
R5860 |
T9288 |
T9285 |
pobj |
antibody,with |
R5861 |
T9289 |
T9273 |
punct |
", ",Immunostaining |
R5937 |
T9373 |
T9371 |
pobj |
tissue,of |
R5941 |
T9377 |
T9375 |
cc |
versus,type |
R5942 |
T9378 |
T9379 |
amod |
homozygous,Cln3Δex7 |
R5943 |
T9379 |
T9380 |
npadvmod |
Cln3Δex7,knock |
R5944 |
T9380 |
T9375 |
conj |
knock,type |
R5945 |
T9381 |
T9379 |
punct |
/,Cln3Δex7 |
R5946 |
T9382 |
T9379 |
nummod |
8,Cln3Δex7 |
R5947 |
T9383 |
T9380 |
punct |
-,knock |
R5948 |
T9384 |
T9380 |
prt |
in,knock |
R5949 |
T9385 |
T9373 |
cc |
or,tissue |
R5950 |
T9386 |
T9387 |
nmod |
CbCln3Δex7,extracts |
R5951 |
T9387 |
T9373 |
conj |
extracts,tissue |
R5952 |
T9388 |
T9386 |
punct |
/,CbCln3Δex7 |
R5953 |
T9389 |
T9386 |
nummod |
8,CbCln3Δex7 |
R5954 |
T9390 |
T9387 |
amod |
cellular,extracts |
R5955 |
T9391 |
T9362 |
auxpass |
are,shown |
R5956 |
T9392 |
T9362 |
punct |
.,shown |
R5957 |
T9394 |
T9395 |
det |
The,band |
R5958 |
T9395 |
T9401 |
nsubj |
band,was |
R1643 |
T2811 |
T2812 |
compound |
enzyme,secretion |
R1644 |
T2812 |
T2813 |
nsubjpass |
secretion,affected |
R1645 |
T2813 |
T2810 |
advcl |
affected,indicating |
R1646 |
T2814 |
T2813 |
auxpass |
was,affected |
R1647 |
T2815 |
T2813 |
neg |
not,affected |
R1648 |
T2816 |
T2801 |
punct |
.,contain |
R1649 |
T2818 |
T2819 |
amod |
Heterozygous,mice |
R1650 |
T2819 |
T2823 |
nsubj |
mice,were |
R1651 |
T2820 |
T2819 |
nmod |
Cln3Δex7,mice |
R1652 |
T2821 |
T2820 |
punct |
/,Cln3Δex7 |
R1653 |
T2822 |
T2820 |
nummod |
8,Cln3Δex7 |
R1654 |
T2824 |
T2819 |
cc |
and,mice |
R1655 |
T2825 |
T2826 |
nmod |
CbCln3Δex7,cells |
R1656 |
T2826 |
T2819 |
conj |
cells,mice |
R1657 |
T2827 |
T2825 |
punct |
/,CbCln3Δex7 |
R1658 |
T2828 |
T2825 |
nummod |
8,CbCln3Δex7 |
R1659 |
T2829 |
T2823 |
acomp |
indistinguishable,were |
R1660 |
T2830 |
T2829 |
prep |
from,indistinguishable |
R1661 |
T2831 |
T2832 |
amod |
wild,type |
R1662 |
T2832 |
T2830 |
pobj |
type,from |
R1663 |
T2833 |
T2832 |
punct |
-,type |
R1664 |
T2834 |
T2823 |
punct |
", ",were |
R1665 |
T2835 |
T2836 |
mark |
as,expected |
R1666 |
T2836 |
T2823 |
advcl |
expected,were |
R1667 |
T2837 |
T2836 |
prep |
for,expected |
R1668 |
T2838 |
T2839 |
det |
a,phenotype |
R1669 |
T2839 |
T2837 |
pobj |
phenotype,for |
R1670 |
T2840 |
T2839 |
amod |
recessive,phenotype |
R1671 |
T2841 |
T2839 |
compound |
disease,phenotype |
R1672 |
T2842 |
T2843 |
punct |
(,shown |
R1673 |
T2843 |
T2836 |
parataxis |
shown,expected |
R1674 |
T2844 |
T2843 |
nsubj |
data,shown |
R1675 |
T2845 |
T2843 |
neg |
not,shown |
R1676 |
T2846 |
T2843 |
punct |
),shown |
R1677 |
T2847 |
T2823 |
punct |
.,were |
R1678 |
T2855 |
T2856 |
amod |
c,t |
R1679 |
T2862 |
T2863 |
amod |
e,a |
R1680 |
T2865 |
T2866 |
advmod |
l,ed |
R1681 |
T2867 |
T2866 |
nsubj |
er,ed |
R1682 |
T2868 |
T2867 |
nmod |
t,er |
R1683 |
T2873 |
T2874 |
nsubjpass |
p,tested |
R1684 |
T2876 |
T2877 |
compound |
sin,D |
R1685 |
T2877 |
T2875 |
compound |
D,processing |
R1686 |
T2878 |
T2873 |
prep |
on,p |