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PMC:539297 JSONTXT

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craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T59 0-8 GO:0016020 denotes Membrane
T60 0-20 GO:0055085 denotes Membrane trafficking
T61 25-38 GO:0005739 denotes mitochondrial
T62 61-70 PR:000022190 denotes subunit c
T63 87-97 UBERON:0002037 denotes cerebellar
T64 121-129 CL:0000540 denotes neuronal
T65 280-284 PR:000005591 denotes CLN3
T66 317-325 PR:000005591 denotes battenin
T67 358-371 GO:0005739 denotes mitochondrial
T68 358-395 PR:000022190 denotes mitochondrial ATP synthase, subunit c
T69 419-422 UBERON:0001017 denotes CNS
T70 419-430 CL:0000117 denotes CNS neurons
T71 456-460 PR:000005591 denotes Cln3
T72 476-480 NCBITaxon:10088 denotes mice
T73 524-531 GO:0010467 denotes express
T74 539-547 PR:000005591 denotes battenin
T75 651-665 CL:0000540 denotes neuronal cells
T76 687-692 NCBITaxon:10088 denotes mouse
T77 693-702 UBERON:0002037 denotes cerebella
T78 743-745 UBERON:0002037 denotes Cb
T79 745-749 PR:000005591 denotes Cln3
T80 790-798 CL:0000540 denotes neuronal
T81 854-859 PR:000010124 denotes MAP-2
T82 864-868 PR:000006252 denotes NeuN
T83 879-885 CL:0000540 denotes neuron
T84 909-911 UBERON:0002037 denotes Cb
T85 911-915 PR:000005591 denotes Cln3
T86 938-945 GO:0010467 denotes express
T87 967-975 PR:000005591 denotes battenin
T88 1017-1033 PR:000022190 denotes ATPase subunit c
T89 1099-1110 PR:000006025 denotes cathepsin D
T90 1205-1214 GO:0005764 denotes lysosomal
T91 1254-1265 GO:0006897 denotes endocytosis
T92 1291-1303 GO:0005739 denotes mitochondria
T93 1388-1397 MOP:0000568 denotes oxidative
T94 1457-1465 PR:000005591 denotes battenin
T95 1482-1495 GO:0005622 denotes intracellular
T96 1482-1495 _FRAGMENT denotes intracellular
T97 1505-1516 GO:0046907 denotes trafficking
T98 1496-1504 GO:0016020 denotes membrane
T99 1496-1516 GO:0055085 denotes membrane trafficking
T100 1521-1534 GO:0005739 denotes mitochondrial
T101 1660-1668 CL:0000540 denotes neuronal
T535 2044-2048 SO:0000704 denotes gene
T536 2067-2072 SO:0000147 denotes exons
T537 2097-2105 SO:0000188 denotes intronic
T538 2247-2260 GO:0044754 denotes autolysosomes
T539 2282-2294 _FRAGMENT denotes subunit c of
T540 2299-2333 PR:000022190 denotes mitochondrial ATP synthase complex
T541 2299-2333 GO:0005753 denotes mitochondrial ATP synthase complex
T542 2390-2393 UBERON:0001017 denotes CNS
T543 2390-2401 CL:0000117 denotes CNS neurons
T544 2431-2439 CL:0000540 denotes neuronal
T545 2461-2475 UBERON:0001016 denotes nervous system
T546 2497-2506 PR:000022190 denotes subunit c
T547 2579-2587 CL:0000540 denotes neuronal
T548 2645-2649 PR:000005591 denotes CLN3
T549 2667-2675 PR:000005591 denotes battenin
T550 2689-2693 PR:000005591 denotes CLN3
T551 2697-2703 PR:000005591 denotes cln3 p
T552 2741-2750 GO:0010467 denotes expressed
T553 2763-2771 GO:0016020 denotes membrane
T554 2806-2814 GO:0005764 denotes lysosome
T555 2825-2834 GO:0031982 denotes vesicular
T556 2855-2863 PR:000005591 denotes Battenin
T557 2919-2923 PR:Q9US09 denotes btn1
T558 2935-2939 PR:000005591 denotes CLN3
T559 2940-2948 SO:0000855 denotes ortholog
T560 2966-2974 PR:000005591 denotes battenin
T561 2978-2987 GO:0005764 denotes lysosomal
T562 2988-3002 GO:0006885 denotes pH homeostasis
T563 3007-3027 GO:0006865 denotes amino acid transport
T564 3070-3078 PR:000005591 denotes battenin
T565 3115-3126 SO:0000704 denotes genetically
T566 3140-3145 NCBITaxon:10088 denotes mouse
T567 3153-3157 PR:000005591 denotes Cln3
T568 3173-3177 NCBITaxon:10088 denotes mice
T569 3220-3227 GO:0010467 denotes express
T570 3251-3259 PR:000005591 denotes battenin
T571 3260-3267 SO:0001060 denotes isoform
T572 3292-3302 GO:0042571 denotes antibodies
T573 3347-3351 PR:000005591 denotes Cln3
T574 3367-3371 NCBITaxon:10088 denotes mice
T575 3418-3427 UBERON:0012101 denotes perinatal
T576 3422-3427 GO:0007567 denotes natal
T577 3437-3446 PR:000022190 denotes subunit c
T578 3496-3504 CL:0000540 denotes neuronal
T579 3627-3640 GO:0005739 denotes mitochondrial
T580 3627-3650 PR:000022190 denotes mitochondrial subunit c
T581 3693-3696 UBERON:0001017 denotes CNS
T582 3693-3704 CL:0000117 denotes CNS neurons
T583 3738-3751 CL:0000540 denotes neuronal cell
T584 3816-3836 GO:0008150 denotes biological processes
T585 3869-3879 UBERON:0002037 denotes cerebellar
T586 3869-3888 CL:1001611 denotes cerebellar neuronal
T587 3915-3919 PR:000005591 denotes Cln3
T588 3935-3939 NCBITaxon:10088 denotes mice
T589 3952-3954 UBERON:0002037 denotes Cb
T590 3954-3958 PR:000005591 denotes Cln3
T591 4034-4053 GO:0043227 denotes membrane organelles
T592 4063-4071 GO:0016020 denotes membrane
T593 4063-4083 GO:0055085 denotes membrane trafficking
T594 4096-4109 GO:0005739 denotes mitochondrial
T595 4143-4145 UBERON:0002037 denotes Cb
T1188 4189-4200 SO:0000704 denotes genetically
T1189 4209-4219 UBERON:0002037 denotes cerebellar
T1190 4258-4265 SO:0000704 denotes genetic
T1191 4267-4273 CL:0000540 denotes neuron
T1192 4324-4339 CL:0000120 denotes granule neurons
T1193 4358-4363 GO:0007567 denotes natal
T1194 4375-4384 UBERON:0002037 denotes cerebella
T1195 4416-4420 PR:000005591 denotes Cln3
T1196 4436-4440 NCBITaxon:10088 denotes mice
T1197 4469-4481 CL:0000001 denotes Primary cell
T1198 4504-4519 CL:0000120 denotes granule neurons
T1199 4525-4535 GO:0009293 denotes transduced
T1200 4541-4551 NCBITaxon:11632 denotes retroviral
T1201 4552-4558 SO:0000440 denotes vector
T1202 4579-4587 SO:0005853 denotes cassette
T1203 4620-4624 NCBITaxon:10633 denotes SV40
T1204 4633-4640 CHEBI:59132 denotes antigen
T1205 4652-4656 CHEBI:42768 denotes G418
T1206 4767-4773 PR:000011141 denotes nestin
T1207 4798-4802 PR:000007939 denotes GFAP
T1208 4983-4987 NCBITaxon:10633 denotes SV40
T1209 4996-5003 CHEBI:59132 denotes antigen
T1210 5004-5014 GO:0010467 denotes expression
T1211 5036-5049 GO:0051301 denotes cell division
T1212 5157-5165 CL:0000540 denotes neuronal
T1213 5182-5190 CHEBI:60004 denotes cocktail
T1214 5215-5221 CL:0000540 denotes neuron
T1215 5265-5271 PR:000011141 denotes nestin
T1216 5272-5282 GO:0010467 denotes expression
T1217 5314-5318 PR:000010124 denotes MAP2
T1218 5323-5327 PR:000006252 denotes NeuN
T1219 5328-5338 GO:0010467 denotes expression
T1220 5361-5371 GO:0010467 denotes expression
T1221 5396-5405 PR:000004967 denotes calbindin
T1594 6156-6158 UBERON:0002037 denotes Cb
T1595 6158-6162 PR:000005591 denotes Cln3
T1596 6175-6182 GO:0010467 denotes express
T1597 6190-6198 PR:000005591 denotes battenin
T1598 6242-6244 UBERON:0002037 denotes Cb
T1599 6245-6249 PR:000005591 denotes Cln3
T1600 6324-6328 PR:000005591 denotes Cln3
T1601 6344-6348 NCBITaxon:10088 denotes mice
T1602 6349-6356 GO:0010467 denotes express
T1603 6366-6370 PR:000005591 denotes Cln3
T1604 6376-6382 GO:0008380 denotes splice
T1605 6403-6411 PR:000005591 denotes battenin
T2386 12657-12659 UBERON:0002037 denotes Cb
T2387 12659-12663 PR:000005591 denotes Cln3
T2388 12677-12688 PR:000006025 denotes cathepsin D
T2389 12711-12720 GO:0031982 denotes vesicular
T2390 12734-12741 GO:0005634 denotes nuclear
T2391 12803-12805 UBERON:0002037 denotes Cb
T2392 12805-12809 PR:000005591 denotes Cln3
T2393 12825-12829 PR:000005591 denotes Cln3
T2394 12836-12842 UBERON:0000479 denotes tissue
T2395 12891-12902 PR:000006025 denotes cathepsin D
T2396 12903-12911 SO:0001060 denotes isoforms
T2397 12923-12934 PR:000006025 denotes Cathepsin D
T2398 12935-12943 SO:0001060 denotes isoforms
T2399 13045-13057 SO:0000984 denotes single chain
T2400 13112-13124 SO:0000985 denotes double-chain
T2401 13171-13173 UBERON:0002037 denotes Cb
T2402 13173-13177 PR:000005591 denotes Cln3
T2403 13193-13197 PR:000005591 denotes Cln3
T2404 13204-13210 UBERON:0000479 denotes tissue
T2405 13401-13412 PR:000006025 denotes cathepsin D
T2406 13473-13477 PR:000005591 denotes Cln3
T2407 13484-13488 NCBITaxon:10088 denotes mice
T2408 13493-13495 UBERON:0002037 denotes Cb
T2409 13495-13499 PR:000005591 denotes Cln3
T2410 14753-14754 PR:000006025 denotes i
T2411 14781-14786 PR:000006025 denotes sin D
T2412 14904-14913 PR:000022190 denotes subunit c
T2413 14929-14940 CHEBI:76219 denotes fluorogenic
T2414 14957-14968 PR:000006025 denotes cathepsin D
T2415 15049-15051 UBERON:0002037 denotes Cb
T2416 15051-15055 PR:000005591 denotes Cln3
T2417 15258-15269 PR:000006025 denotes cathepsin D
T2418 15323-15325 UBERON:0002037 denotes Cb
T2419 15325-15329 PR:000005591 denotes Cln3
T3136 16192-16199 GO:0005634 denotes nuclear
T3137 16226-16228 UBERON:0002037 denotes Cb
T3138 16228-16232 PR:000005591 denotes Cln3
T3128 15776-15787 GO:0005802 denotes trans-Golgi
T3129 15880-15897 CHEBI:16249 denotes protein disulfide
T3130 15915-15920 PR:000008131 denotes GM130
T3131 15965-15974 GO:0005764 denotes lysosomal
T3132 16000-16006 PR:000002061 denotes Lamp 2
T3133 16054-16056 UBERON:0002037 denotes Cb
T3134 16056-16060 PR:000005591 denotes Cln3
T3135 16141-16150 GO:0005764 denotes lysosomes
T3139 16239-16248 GO:0005764 denotes lysosomes
T3140 16279-16287 GO:0031982 denotes vesicles
T3141 16330-16339 GO:0005737 denotes cytoplasm
T3142 16362-16368 PR:000002060 denotes Lamp 1
T3143 16439-16445 PR:000002060 denotes Lamp 1
T3144 16450-16456 PR:000002061 denotes Lamp 2
T3145 16519-16521 UBERON:0002037 denotes Cb
T3146 16521-16525 PR:000005591 denotes Cln3
T3147 16618-16627 GO:0005764 denotes lysosomes
T3148 16732-16735 CHEBI:37958 denotes dye
T3149 16770-16776 CHEBI:37527 denotes acidic
T3150 16830-16839 GO:0005764 denotes lysosomal
T3151 16887-16896 GO:0005764 denotes lysosomal
T3152 17823-17824 UBERON:0002037 denotes C
T3153 17824-17825 GO:0005764 denotes o
T3154 17832-17833 GO:0042571 denotes t
T3155 17848-17849 GO:0005634 denotes e
T3156 17854-17855 GO:0005634 denotes l
T3157 17874-17878 PR:000006901 denotes EEA1
T3158 17924-17947 GO:0006907 denotes fluid-phase endocytosis
T3159 17979-17981 UBERON:0002037 denotes Cb
T3160 17981-17985 PR:000005591 denotes Cln3
T3161 18014-18021 CHEBI:52071 denotes dextran
T3162 18022-18026 CHEBI:37926 denotes FITC
T3163 18097-18104 CHEBI:52071 denotes dextran
T3164 18105-18109 CHEBI:37926 denotes FITC
T3165 18178-18187 GO:0006897 denotes endocytic
T3166 18178-18196 GO:0030139 denotes endocytic vesicles
T3167 18228-18235 GO:0005634 denotes nuclear
T3168 18264-18266 UBERON:0002037 denotes Cb
T3169 18266-18270 PR:000005591 denotes Cln3
T3170 18320-18327 CHEBI:52071 denotes dextran
T3171 18328-18332 CHEBI:37926 denotes FITC
T3172 18362-18370 GO:0031982 denotes vesicles
T3192 19693-19705 GO:0005739 denotes mitochondria
T3193 19762-19775 GO:0005739 denotes Mitochondrial
T3194 19812-19814 UBERON:0002037 denotes Cb
T3195 19814-19818 PR:000005591 denotes Cln3
T3196 19915-19917 UBERON:0002037 denotes Cb
T3197 19917-19921 PR:000005591 denotes Cln3
T3198 20046-20063 CHEBI:16240 denotes hydrogen peroxide
T3199 20140-20150 GO:0008152 denotes metabolism
T3200 20155-20164 MOP:0000568 denotes oxidative
T3201 20226-20239 GO:0005739 denotes mitochondrial
T3202 20263-20265 UBERON:0002037 denotes Cb
T3203 20265-20269 PR:000005591 denotes Cln3
T6601 31006-31011 GO:0040007 denotes grown
T6602 31089-31101 CHEBI:16842 denotes formaldehyde
T6603 31151-31159 CHEBI:17790 denotes methanol
T6604 31160-31167 CHEBI:15347 denotes acetone
T6605 31222-31230 GO:0042571 denotes antibody
T6606 31422-31430 GO:0042571 denotes antibody
T6607 31442-31454 CHEBI:9750 denotes Triton X-100
T6608 31500-31506 NCBITaxon:27592 denotes bovine
T6609 31507-31512 UBERON:0001977 denotes serum
T6610 31507-31520 PR:000003918 denotes serum albumin
T6611 31558-31566 GO:0042571 denotes antibody
T6612 31615-31623 GO:0042571 denotes antibody
T6613 32183-32187 CHEBI:9754 denotes Tris
T6614 32200-32212 CHEBI:9750 denotes Triton X-100
T6615 32214-32222 GO:0016020 denotes membrane
T6616 32305-32316 CHEBI:7989 denotes pepstatin A
T6617 32345-32354 CHEBI:6426 denotes leupeptin
T6618 32599-32602 CHEBI:8984 denotes SDS
T6619 32665-32669 CHEBI:9754 denotes tris
T6620 32670-32677 CHEBI:46760 denotes tricine
T6621 32678-32681 CHEBI:8984 denotes SDS
T6622 32706-32715 PR:000022190 denotes subunit c
T4173 22531-22539 CL:0000540 denotes neuronal
T4152 21950-21952 UBERON:0002037 denotes Cb
T4153 21952-21956 PR:000005591 denotes Cln3
T4154 21963-21973 UBERON:0002037 denotes cerebellar
T4155 22010-22021 SO:0000704 denotes genetically
T4156 22031-22037 CL:0000540 denotes neuron
T4157 22080-22082 UBERON:0002037 denotes Cb
T4158 22082-22086 PR:000005591 denotes Cln3
T4159 22099-22106 GO:0010467 denotes express
T4160 22114-22122 PR:000005591 denotes battenin
T4161 22141-22154 GO:0005739 denotes mitochondrial
T4162 22141-22171 PR:000022190 denotes mitochondrial ATPase subunit c
T4163 22191-22205 GO:0007569 denotes aging of cells
T4164 22277-22286 _FRAGMENT denotes endosomal
T4165 22297-22305 GO:0010008 denotes membrane
T4166 22306-22317 GO:0016197 denotes trafficking
T4167 22287-22305 GO:0005765 denotes lysosomal membrane
T4168 22287-22296 _FRAGMENT denotes lysosomal
T4169 22306-22317 GO:0007041 denotes trafficking
T4170 22297-22317 GO:0055085 denotes membrane trafficking
T4171 22330-22343 GO:0005739 denotes mitochondrial
T4172 22370-22379 PR:000022190 denotes subunit c
T4174 22559-22570 PR:000006025 denotes cathepsin D
T4175 22613-22615 UBERON:0002037 denotes Cb
T4176 22615-22619 PR:000005591 denotes Cln3
T4177 22636-22640 PR:000005591 denotes Cln3
T4178 22647-22651 NCBITaxon:10088 denotes mice
T4179 22676-22685 GO:0031982 denotes vesicular
T4180 22676-22697 GO:0016192 denotes vesicular trafficking
T4181 22705-22714 GO:0005764 denotes lysosomal
T4182 22744-22755 PR:000006025 denotes cathepsin D
T4183 22784-22788 PR:000005591 denotes CLN3
T4184 22793-22803 GO:0010467 denotes expression
T4185 22829-22838 GO:0005764 denotes lysosomal
T4186 22846-22857 PR:000006025 denotes cathepsin D
T4187 22879-22888 GO:0005764 denotes lysosomal
T4188 22889-22903 GO:0006885 denotes pH homeostasis
T4189 22956-22967 PR:000006023 denotes cathepsin B
T4190 22976-22980 PR:000016585 denotes CLN2
T4191 22997-23001 PR:000016585 denotes TPPI
T4192 23063-23074 PR:000006025 denotes cathepsin D
T4193 23127-23129 UBERON:0002037 denotes Cb
T4217 23964-23973 PR:000022190 denotes subunit c
T4218 23988-24001 GO:0005739 denotes Mitochondrial
T4219 24074-24080 NCBITaxon:33208 denotes animal
T4220 24137-24146 GO:0006914 denotes autophagy
T4221 24150-24159 GO:0005764 denotes lysosomal
T4222 24217-24225 PR:000005591 denotes battenin
T4223 24248-24261 GO:0005739 denotes mitochondrial
T4224 24303-24316 GO:0005739 denotes mitochondrial
T4225 24343-24352 _FRAGMENT denotes transport
T4226 24368-24370 _FRAGMENT denotes of
T4227 24385-24393 GO:0015031 denotes proteins
T4228 24357-24393 GO:0034982 denotes processing of mitochondrial proteins
T4229 24371-24384 GO:0005739 denotes mitochondrial
T4230 24408-24410 UBERON:0002037 denotes Cb
T4231 24410-24414 PR:000005591 denotes Cln3
T4232 24415-24423 CL:0000540 denotes neuronal
T4233 24440-24448 PR:000005591 denotes battenin
T4234 24477-24482 _FRAGMENT denotes early
T4235 24492-24501 GO:0005769 denotes endosomes
T4236 24487-24501 GO:0005770 denotes late endosomes
T4237 24503-24511 PR:000005591 denotes Battenin
T4238 24541-24543 UBERON:0002037 denotes Cb
T4239 24543-24547 PR:000005591 denotes Cln3
T4240 24554-24562 CL:0000540 denotes neuronal
T4241 24654-24663 GO:0005768 denotes endosomal
T4242 24690-24698 PR:000005591 denotes battenin
T4243 24794-24798 PR:000005591 denotes CLN3
T4244 24799-24807 PR:000005591 denotes battenin
T4245 24873-24887 GO:0005770 denotes late endosomes
T4246 24892-24901 GO:0005764 denotes lysosomes
T4247 24909-24917 CL:0000540 denotes neuronal
T4248 24936-24945 GO:0005764 denotes lysosomes
T4249 24968-24977 GO:0005768 denotes endosomes
T4250 24988-24995 CL:0000540 denotes neurons
T4251 25030-25038 PR:000005591 denotes battenin
T4252 25062-25071 GO:0031982 denotes vesicular
T4253 25102-25110 GO:0016020 denotes membrane
T4254 25102-25122 GO:0055085 denotes membrane trafficking
T4255 25156-25165 GO:0031982 denotes vesicular
T4256 25156-25175 GO:0016192 denotes vesicular transport
T4257 25156-25165 _FRAGMENT denotes vesicular
T4258 25183-25189 GO:0006906 denotes fusion
T4259 25191-25200 GO:0006897 denotes Endocytic
T4260 25205-25214 GO:0005764 denotes lysosomal
T4261 25244-25257 GO:0005739 denotes mitochondrial
T4262 25244-25267 GO:0000422 denotes mitochondrial autophagy
T5155 25821-25829 GO:0042571 denotes Antibody
T5156 25848-25856 CHEBI:33893 denotes reagents
T5157 25857-25863 PR:000011141 denotes Nestin
T5158 25871-25874 NCBITaxon:10114 denotes Rat
T5159 25890-25896 PR:000002060 denotes Lamp 1
T5160 25924-25930 PR:000002061 denotes Lamp 2
T5161 25966-25976 GO:0042571 denotes antibodies
T5162 26163-26172 PR:000022190 denotes subunit c
T5163 26173-26181 GO:0042571 denotes antibody
T5164 26278-26288 GO:0042571 denotes antibodies
T5165 26306-26310 PR:000007939 denotes GFAP
T5166 26339-26348 PR:000004967 denotes calbindin
T5167 26366-26370 PR:000006252 denotes NeuN
T5168 26393-26397 NCBITaxon:10633 denotes SV40
T5169 26400-26407 CHEBI:59132 denotes antigen
T5170 26455-26466 PR:000006025 denotes cathepsin D
T5171 26511-26523 CHEBI:18070 denotes cytochrome c
T5172 26511-26542 PR:000005776 denotes cytochrome c oxidase subunit IV
T5173 26544-26548 PR:000005776 denotes cox4
T5174 26634-26639 PR:000008131 denotes GM130
T5175 26653-26665 GO:0009293 denotes Transduction
T5176 26675-26682 PR:000028799 denotes tubulin
T5177 26702-26707 PR:000008813 denotes grp75
T5178 26728-26742 GO:0005769 denotes early endosome
T5179 26728-26752 PR:000006901 denotes early endosome antigen-1
T5180 26743-26750 CHEBI:59132 denotes antigen
T5181 26754-26758 PR:000006901 denotes EEA1
T5182 26797-26802 PR:000013639 denotes rab 7
T5183 26866-26876 GO:0042571 denotes antibodies
T5184 26940-26950 MOP:0000779 denotes conjugated
T5185 26961-26971 GO:0042571 denotes antibodies
T5186 27058-27064 CHEBI:15956 denotes biotin
T5187 27106-27113 CHEBI:52071 denotes dextran
T5188 27114-27118 CHEBI:37926 denotes FITC
T5189 27132-27154 CHEBI:52117 denotes Lysotracker Red DND-99
T5620 27231-27233 UBERON:0002037 denotes Cb
T5621 27233-27237 PR:000005591 denotes Cln3
T5622 27238-27248 UBERON:0002037 denotes cerebellar
T5623 27238-27257 CL:1001611 denotes cerebellar neuronal
T5624 27274-27278 PR:000005591 denotes Cln3
T5625 27294-27298 NCBITaxon:10088 denotes mice
T5626 27466-27476 UBERON:0002037 denotes cerebellar
T5627 27466-27491 CL:0001031 denotes cerebellar granule neuron
T5628 27520-27525 GO:0007567 denotes natal
T5629 27537-27546 UBERON:0002037 denotes cerebella
T5630 27634-27641 CHEBI:17234 denotes glucose
T5631 27643-27647 UBERON:0002415 denotes Tail
T5632 27681-27688 SO:0001026 denotes genomic
T5633 27727-27734 PR:000027795 denotes Trypsin
T5634 27827-27836 UBERON:0002037 denotes cerebella
T5635 27954-27963 UBERON:0002037 denotes cerebella
T5636 28000-28003 CHEBI:16526 denotes CO2
T5637 28008-28022 CL:0000120 denotes granule neuron
T5638 28110-28116 NCBITaxon:27592 denotes bovine
T5639 28117-28122 UBERON:0001977 denotes serum
T5640 28164-28167 CHEBI:32588 denotes KCl
T5641 28227-28237 NCBITaxon:11632 denotes retrovirus
T5642 28238-28249 GO:0009293 denotes transducing
T5643 28294-28301 CHEBI:59132 denotes antigen
T5644 28318-28326 CHEBI:7507 denotes neomycin
T5645 28338-28346 SO:0005853 denotes cassette
T5646 28549-28553 CHEBI:42768 denotes G418
T5647 28808-28810 UBERON:0002037 denotes Cb
T5648 28810-28814 PR:000005591 denotes Cln3
T5649 28827-28829 UBERON:0002037 denotes Cb
T5650 28936-28939 CHEBI:16526 denotes CO2
T5651 29043-29051 CL:0000540 denotes Neuronal
T5652 29120-29128 CHEBI:60004 denotes cocktail
T5653 29139-29144 PR:000007479 denotes α-FGF
T5654 29153-29157 CHEBI:48518 denotes IBMX
T5655 29166-29169 CHEBI:37537 denotes TPA
T5656 29177-29186 CHEBI:42471 denotes forskolin
T5657 29193-29201 CHEBI:18243 denotes dopamine
T6145 29511-29518 SO:0000112 denotes primers
T6146 29612-29614 SO:0000028 denotes bp
T6147 29764-29768 PR:000005591 denotes Cln3
T6148 29776-29783 SO:0000112 denotes primers
T6342 29837-29846 PR:000022190 denotes Subunit c
T6343 30251-30263 CHEBI:16842 denotes formaldehyde
T6344 30358-30367 PR:000022190 denotes subunit c
T6345 30487-30501 CHEBI:64276 denotes glutaraldehyde
T6346 30531-30541 CHEBI:16223 denotes cacodylate
T6347 30835-30843 GO:0042571 denotes antibody
T7096 32745-32756 PR:000006025 denotes Cathepsin D
T7097 32779-32785 UBERON:0000479 denotes tissue
T7098 32954-32958 CHEBI:9754 denotes Tris
T7099 32973-32985 CHEBI:9750 denotes Triton X-100
T7100 33247-33258 PR:000006025 denotes cathepsin D
T7101 33278-33289 CHEBI:76219 denotes Fluorogenic
T7102 33300-33311 PR:000006025 denotes Cathepsin D
T7316 33687-33696 GO:0005764 denotes Lysosomal
T7317 33710-33726 GO:0006897 denotes endocytic uptake
T7318 33815-33820 GO:0040007 denotes grown
T7319 33943-33950 CHEBI:52071 denotes dextran
T7320 33951-33955 CHEBI:37926 denotes FITC
T7321 34129-34132 CHEBI:37958 denotes dye
T7322 34163-34175 CHEBI:16842 denotes formaldehyde
T7476 34370-34382 GO:0005739 denotes mitochondria
T7477 34526-34538 GO:0005739 denotes mitochondria
T7478 34596-34608 GO:0005739 denotes mitochondria
T7479 34872-34885 GO:0005739 denotes mitochondrial
T7609 35333-35340 CHEBI:33893 denotes Reagent
T7610 35368-35378 GO:0019835 denotes cell lysis
T7768 35677-35694 CHEBI:16240 denotes Hydrogen peroxide
T7769 35882-35899 CHEBI:16240 denotes hydrogen peroxide
T7770 35968-35985 CHEBI:16240 denotes hydrogen peroxide
T7771 36084-36102 GO:0008283 denotes Cell Proliferation
T7898 5427-5435 CL:0000540 denotes Neuronal
T7899 5443-5453 GO:0010467 denotes expression
T7900 5457-5459 UBERON:0002037 denotes Cb
T7901 5459-5463 PR:000005591 denotes Cln3
T7902 5493-5495 UBERON:0002037 denotes Cb
T7903 5495-5499 PR:000005591 denotes Cln3
T7904 5543-5553 GO:0042571 denotes antibodies
T7905 5564-5566 UBERON:0002037 denotes Cb
T7906 5566-5570 PR:000005591 denotes Cln3
T7907 5593-5599 PR:000011141 denotes nestin
T7908 5600-5610 GO:0010467 denotes expression
T7909 5623-5627 PR:000007939 denotes GFAP
T7910 5628-5638 GO:0010467 denotes expression
T7911 5662-5670 CL:0000540 denotes neuronal
T7912 5731-5739 CHEBI:60004 denotes cocktail
T7913 5755-5757 UBERON:0002037 denotes Cb
T7914 5757-5761 PR:000005591 denotes Cln3
T7915 5780-5786 CL:0000540 denotes neuron
T7916 5817-5828 GO:0044297 denotes cell bodies
T7917 5846-5855 GO:0042995 denotes processes
T7918 5861-5865 PR:000010124 denotes MAP2
T7919 5874-5878 PR:000006252 denotes NeuN
T7920 5883-5893 GO:0010467 denotes expression
T7921 5909-5911 UBERON:0002037 denotes Cb
T7922 5911-5915 PR:000005591 denotes Cln3
T7923 5946-5961 CL:0000121 denotes Purkinje neuron
T7924 5969-5978 PR:000004967 denotes calbindin
T7925 5984-5986 UBERON:0002037 denotes Cb
T7926 5986-5990 PR:000005591 denotes Cln3
T7927 6003-6005 UBERON:0002037 denotes Cb
T7928 6005-6009 PR:000005591 denotes Cln3
T8165 7822-7826 PR:000005591 denotes Cln3
T8166 7860-7862 UBERON:0002037 denotes Cb
T8167 7862-7866 PR:000005591 denotes Cln3
T8168 7879-7883 PR:000005591 denotes Cln3
T8169 7890-7897 SO:0001030 denotes forward
T8170 7899-7903 SO:0000147 denotes Exon
T8171 7907-7914 SO:0001031 denotes reverse
T8172 7918-7930 SO:0000006 denotes PCR products
T8173 8006-8011 UBERON:0000955 denotes brain
T8174 8031-8036 UBERON:0000955 denotes Brain
T8175 8054-8075 SO:0000006 denotes PCR reaction products
T8176 8107-8123 CHEBI:4883 denotes ethidium-bromide
T8177 8132-8139 CHEBI:2511 denotes agarose
T8178 8159-8170 SO:0000006 denotes PCR product
T8179 8289-8295 GO:0008380 denotes splice
T8367 8316-8324 PR:000005591 denotes Battenin
T8368 8329-8338 GO:0005764 denotes lysosomal
T8369 8343-8352 GO:0005768 denotes endosomal
T8370 8400-8402 UBERON:0002037 denotes Cb
T8371 8402-8406 PR:000005591 denotes Cln3
T8372 8413-8423 UBERON:0002037 denotes cerebellar
T8373 8475-8477 UBERON:0002037 denotes Cb
T8374 8477-8481 PR:000005591 denotes Cln3
T8375 8509-8511 UBERON:0002037 denotes Cb
T8376 8511-8515 PR:000005591 denotes Cln3
T8377 8530-8540 UBERON:0002037 denotes cerebellar
T8378 8588-8597 GO:0005764 denotes lysosomes
T8379 8599-8605 PR:000002060 denotes Lamp 1
T8380 8608-8623 GO:0005769 denotes early endosomes
T8381 8625-8629 PR:000006901 denotes EEA1
T8382 8636-8650 GO:0005770 denotes late endosomes
T8383 8652-8656 PR:000013639 denotes Rab7
T8384 8706-8710 PR:000006901 denotes EEA1
T8385 8738-8742 PR:000013639 denotes Rab7
T8386 8875-8879 PR:000013639 denotes Rab7
T8387 8926-8932 PR:000002060 denotes Lamp 1
T8388 8993-8995 UBERON:0002037 denotes Cb
T8389 8995-8999 PR:000005591 denotes Cln3
T8390 9067-9071 PR:000006901 denotes EEA1
T8391 9076-9080 PR:000013639 denotes Rab7
T8392 9098-9104 PR:000002060 denotes Lamp 1
T8393 9178-9184 PR:000002060 denotes Lamp 1
T8394 9189-9193 PR:000006901 denotes EEA1
T8395 9227-9231 PR:000013639 denotes Rab7
T8396 9283-9285 UBERON:0002037 denotes Cb
T8397 9285-9289 PR:000005591 denotes Cln3
T8398 9328-9330 UBERON:0002037 denotes Cb
T8399 9330-9334 PR:000005591 denotes Cln3
T8709 10430-10439 PR:000022190 denotes Subunit c
T8710 10467-10469 UBERON:0002037 denotes Cb
T8711 10469-10473 PR:000005591 denotes Cln3
T8712 10480-10490 UBERON:0002037 denotes cerebellar
T8713 10510-10519 PR:000022190 denotes Subunit c
T8714 10606-10608 UBERON:0002037 denotes Cb
T8715 10608-10612 PR:000005591 denotes Cln3
T8716 10655-10657 UBERON:0002037 denotes Cb
T8717 10657-10661 PR:000005591 denotes Cln3
T8718 10684-10693 PR:000022190 denotes subunit c
T8719 10740-10742 UBERON:0002037 denotes Cb
T8720 10742-10746 PR:000005591 denotes Cln3
T8721 10785-10794 PR:000022190 denotes subunit c
T8722 10910-10919 PR:000022190 denotes subunit c
T8723 10996-11005 PR:000022190 denotes subunit c
T8724 11219-11228 PR:000022190 denotes subunit c
T8725 11240-11245 PR:000022190 denotes sub c
T8726 11289-11291 UBERON:0002037 denotes Cb
T8727 11291-11295 PR:000005591 denotes Cln3
T8728 11347-11356 PR:000022190 denotes subunit c
T8729 11445-11457 CHEBI:18070 denotes cytochrome c
T8730 11445-11476 PR:000005776 denotes cytochrome c oxidase subunit IV
T8731 11480-11484 PR:000005776 denotes cox4
T8732 11506-11516 GO:0016234 denotes inclusions
T8733 11553-11555 UBERON:0002037 denotes Cb
T8734 11555-11559 PR:000005591 denotes Cln3
T8735 11590-11603 GO:0005776 denotes autophagosome
T8736 11624-11632 GO:0016020 denotes membrane
T8737 11670-11682 GO:0005739 denotes mitochondria
T8738 11689-11697 CHEBI:10545 denotes electron
T8739 11689-11709 GO:0031045 denotes electron dense cores
T8740 11750-11759 GO:0031982 denotes vesicular
T8741 11780-11788 CHEBI:10545 denotes electron
T8742 11795-11804 GO:0016234 denotes inclusion
T8743 11858-11859 GO:0005739 denotes M
T8744 11861-11873 GO:0005739 denotes mitochondria
T9165 13625-13636 PR:000006025 denotes Cathepsin D
T9166 13693-13695 UBERON:0002037 denotes Cb
T9167 13695-13699 PR:000005591 denotes Cln3
T9168 13758-13760 UBERON:0002037 denotes Cb
T9169 13760-13764 PR:000005591 denotes Cln3
T9170 13797-13808 PR:000006025 denotes cathepsin D
T9171 13809-13817 GO:0042571 denotes antibody
T9172 13866-13877 PR:000006025 denotes cathepsin D
T9173 13896-13898 UBERON:0002037 denotes Cb
T9174 13898-13902 PR:000005591 denotes Cln3
T9175 13941-13948 GO:0005634 denotes nuclear
T9176 13953-13964 GO:0005737 denotes cytoplasmic
T9177 13982-13993 PR:000006025 denotes Cathepsin D
T9178 14015-14017 UBERON:0002037 denotes Cb
T9179 14017-14021 PR:000005591 denotes Cln3
T9180 14067-14074 GO:0005634 denotes nuclear
T9181 14086-14097 GO:0005737 denotes cytoplasmic
T9182 14137-14139 UBERON:0002037 denotes Cb
T9183 14139-14143 PR:000005591 denotes Cln3
T9184 14179-14190 PR:000006025 denotes Cathepsin D
T9185 14247-14251 PR:000005591 denotes Cln3
T9186 14267-14273 UBERON:0000479 denotes tissue
T9187 14277-14279 UBERON:0002037 denotes Cb
T9188 14279-14283 PR:000005591 denotes Cln3
T9189 14331-14342 PR:000006025 denotes cathepsin D
T9190 14416-14422 UBERON:0000479 denotes tissue
T9191 14555-14559 PR:000005591 denotes Cln3
T9192 14570-14572 UBERON:0002037 denotes Cb
T9193 14572-14576 PR:000005591 denotes Cln3
T9194 14664-14676 SO:0000985 denotes double-chain
T9195 14721-14733 SO:0000984 denotes single-chain
T9510 16967-16973 PR:000002061 denotes Lamp 2
T9511 17011-17013 UBERON:0002037 denotes Cb
T9512 17013-17017 PR:000005591 denotes Cln3
T9513 17024-17033 GO:0005764 denotes lysosomes
T9514 17034-17043 GO:0005764 denotes Lysosomal
T9515 17081-17083 UBERON:0002037 denotes Cb
T9516 17083-17087 PR:000005591 denotes Cln3
T9517 17131-17137 PR:000002061 denotes Lamp 2
T9518 17138-17146 GO:0042571 denotes antibody
T9519 17169-17172 CHEBI:37958 denotes dye
T9520 17205-17212 GO:0005634 denotes nuclear
T9521 17223-17232 GO:0005764 denotes lysosomes
T9522 17247-17256 GO:0005764 denotes lysosomes
T9523 17264-17276 _FRAGMENT denotes periphery of
T9524 17287-17292 GO:0071944 denotes cells
T9525 17294-17296 UBERON:0002037 denotes Cb
T9526 17296-17300 PR:000005591 denotes Cln3
T9527 17377-17379 UBERON:0002037 denotes Cb
T9528 17379-17383 PR:000005591 denotes Cln3
T9529 17420-17428 GO:0031982 denotes vesicles
T9530 17451-17458 GO:0005634 denotes nuclear
T9531 17471-17477 PR:000002061 denotes Lamp 2
T9532 17560-17567 GO:0005634 denotes nuclear
T9533 17593-17595 UBERON:0002037 denotes Cb
T9534 17595-17599 PR:000005591 denotes Cln3
T9535 17696-17699 CHEBI:37958 denotes dye
T9536 17726-17728 UBERON:0002037 denotes Cb
T9537 17728-17732 PR:000005591 denotes Cln3
T9758 18429-18440 GO:0006897 denotes Endocytosis
T9759 18483-18485 UBERON:0002037 denotes Cb
T9760 18485-18489 PR:000005591 denotes Cln3
T9761 18502-18509 CHEBI:52071 denotes Dextran
T9762 18510-18514 CHEBI:37926 denotes FITC
T9763 18564-18566 UBERON:0002037 denotes Cb
T9764 18566-18570 PR:000005591 denotes Cln3
T9765 18617-18619 UBERON:0002037 denotes Cb
T9766 18619-18623 PR:000005591 denotes Cln3
T9767 18658-18660 UBERON:0002037 denotes Cb
T9768 18660-18664 PR:000005591 denotes Cln3
T9769 18695-18702 CHEBI:52071 denotes dextran
T9770 18703-18707 CHEBI:37926 denotes FITC
T9771 18736-18743 GO:0005634 denotes nuclear
T9772 18754-18763 GO:0031982 denotes vesicular
T9773 18795-18803 GO:0031982 denotes vesicles
T9774 18848-18855 CHEBI:52071 denotes dextran
T9775 18856-18860 CHEBI:37926 denotes FITC
T9776 18889-18891 UBERON:0002037 denotes Cb
T9777 18891-18895 PR:000005591 denotes Cln3
T9778 18979-18987 GO:0031982 denotes vesicles
T9779 19002-19009 GO:0005634 denotes nuclear
T10001 20293-20306 GO:0005739 denotes Mitochondrial
T10002 20373-20375 UBERON:0002037 denotes Cb
T10003 20375-20379 PR:000005591 denotes Cln3
T10004 20452-20454 UBERON:0002037 denotes Cb
T10005 20454-20458 PR:000005591 denotes Cln3
T10006 20465-20478 GO:0005739 denotes mitochondrial
T10007 20525-20553 GO:0005743 denotes inner mitochondrial membrane
T10008 20562-20567 PR:000008813 denotes grp75
T10009 20603-20615 GO:0005739 denotes mitochondria
T10010 20644-20646 UBERON:0002037 denotes Cb
T10011 20646-20650 PR:000005591 denotes Cln3
T10012 20688-20700 GO:0005739 denotes mitochondria
T10013 20702-20704 UBERON:0002037 denotes Cb
T10014 20704-20708 PR:000005591 denotes Cln3
T10015 20737-20750 GO:0005739 denotes Mitochondrial
T10016 20829-20831 UBERON:0002037 denotes Cb
T10017 20831-20835 PR:000005591 denotes Cln3
T10018 20842-20854 GO:0005739 denotes mitochondria
T10019 20976-20978 UBERON:0002037 denotes Cb
T10020 20978-20982 PR:000005591 denotes Cln3
T10021 21065-21067 UBERON:0002037 denotes Cb
T10022 21067-21071 PR:000005591 denotes Cln3
T10023 21123-21125 UBERON:0002037 denotes Cb
T10024 21125-21129 PR:000005591 denotes Cln3
T10025 21295-21297 UBERON:0002037 denotes Cb
T10026 21297-21301 PR:000005591 denotes Cln3
T10027 21503-21520 CHEBI:16240 denotes hydrogen peroxide
T10028 21552-21554 UBERON:0002037 denotes Cb
T10029 21554-21558 PR:000005591 denotes Cln3
T10030 21602-21611 MOP:0000568 denotes oxidative
T10031 21622-21639 CHEBI:16240 denotes hydrogen peroxide
T10032 21698-21700 UBERON:0002037 denotes Cb
T10033 21700-21704 PR:000005591 denotes Cln3
T10034 21762-21766 CHEBI:16240 denotes H2O2
T10035 21787-21789 UBERON:0002037 denotes Cb
T10036 21789-21793 PR:000005591 denotes Cln3
T10037 21852-21856 CHEBI:16240 denotes H2O2
T5013 25329-25337 GO:0016020 denotes membrane
T5014 25329-25349 GO:0055085 denotes membrane trafficking
T5015 25354-25367 GO:0005739 denotes mitochondrial
T5016 25401-25403 UBERON:0002037 denotes Cb
T5017 25403-25407 PR:000005591 denotes Cln3
T5018 25454-25462 CL:0000540 denotes neuronal
T5019 25509-25525 GO:0031988 denotes membrane vesicle
T5020 25518-25535 GO:0016192 denotes vesicle transport
T5021 25555-25565 GO:0008152 denotes metabolism
T5022 25588-25595 CL:0000540 denotes neurons
T5023 25611-25619 PR:000005591 denotes battenin
T5024 25689-25691 UBERON:0002037 denotes Cb
T5025 25691-25695 PR:000005591 denotes Cln3
T5026 25702-25712 UBERON:0002037 denotes cerebellar
T5027 25770-25778 PR:000005591 denotes battenin
T6139 29234-29241 SO:0001026 denotes Genomic
T6140 29265-29269 UBERON:0002415 denotes tail
T6141 29332-29336 PR:000005591 denotes Cln3
T6142 29352-29358 SO:0001023 denotes allele
T6143 29393-29400 SO:0000112 denotes primers
T6144 29490-29492 SO:0000028 denotes bp
T2362 11911-11913 UBERON:0002037 denotes Cb
T2363 11913-11917 PR:000005591 denotes Cln3
T2364 11934-11938 PR:000005591 denotes Cln3
T2365 11954-11958 NCBITaxon:10088 denotes mice
T2366 11967-11978 PR:000006025 denotes cathepsin D
T2367 12025-12034 PR:000022190 denotes subunit c
T2368 12077-12088 PR:000006025 denotes cathepsin D
T2369 12126-12148 PR:000022190 denotes ATP synthase subunit c
T2370 12168-12176 GO:0005764 denotes lysosome
T2371 12199-12210 PR:000006025 denotes cathepsin D
T2372 12250-12252 UBERON:0002037 denotes Cb
T2373 12252-12256 PR:000005591 denotes Cln3
T2374 12273-12277 PR:000005591 denotes Cln3
T2375 12284-12288 NCBITaxon:10088 denotes mice
T2376 12300-12311 PR:000006025 denotes cathepsin D
T2377 12312-12320 GO:0042571 denotes antibody
T2378 12363-12374 PR:000006025 denotes cathepsin D
T2379 12375-12383 SO:0001060 denotes isoforms
T2380 12428-12430 UBERON:0002037 denotes Cb
T2381 12430-12434 PR:000005591 denotes Cln3
T2382 12462-12469 GO:0005634 denotes nuclear
T2383 12483-12492 GO:0031982 denotes vesicular
T2384 12493-12504 PR:000006025 denotes cathepsin D
T2385 12614-12622 GO:0005764 denotes lysosome
T529 1700-1708 CL:0000540 denotes neuronal
T530 1888-1897 GO:0050890 denotes cognitive
T531 1917-1922 GO:0016265 denotes death
T532 1940-1947 SO:0000704 denotes genetic
T533 2011-2018 SO:0001026 denotes genomic
T534 2039-2043 PR:000005591 denotes CLN3
T1606 6448-6456 GO:0042571 denotes antibody
T1607 6533-6535 UBERON:0002037 denotes Cb
T1608 6535-6539 PR:000005591 denotes Cln3
T1609 6569-6577 PR:000005591 denotes battenin
T1610 6639-6643 PR:000005591 denotes Cln3
T1611 6649-6657 SO:0001060 denotes isoforms
T1612 6774-6778 PR:000005591 denotes Cln3
T1613 6794-6799 UBERON:0000955 denotes brain
T1614 6874-6882 PR:000005591 denotes battenin
T1615 6905-6907 UBERON:0002037 denotes Cb
T1616 6907-6911 PR:000005591 denotes Cln3
T1617 6953-6964 GO:0005737 denotes cytoplasmic
T1618 6966-6975 GO:0031982 denotes vesicular
T1619 7071-7080 GO:0005764 denotes lysosomal
T1620 7089-7094 PR:000002060 denotes Lamp1
T1621 7134-7148 GO:0005769 denotes early endosome
T1622 7134-7158 PR:000006901 denotes early endosome antigen 1
T1623 7149-7156 CHEBI:59132 denotes antigen
T1624 7160-7164 PR:000006901 denotes EEA1
T1625 7174-7188 GO:0005770 denotes late endosomal
T1626 7197-7201 PR:000013639 denotes Rab7
T1627 7251-7270 GO:0055037 denotes recycling endosomes
T1628 7289-7300 PR:000016261 denotes transferrin
T1629 7354-7359 PR:000002060 denotes Lamp1
T1630 7364-7368 PR:000006901 denotes EEA1
T1631 7428-7430 UBERON:0002037 denotes Cb
T1632 7430-7434 PR:000005591 denotes Cln3
T1633 7462-7469 GO:0005634 denotes nuclear
T1634 7510-7514 PR:000013639 denotes Rab7
T1635 7566-7568 UBERON:0002037 denotes Cb
T1636 7568-7572 PR:000005591 denotes Cln3
T1637 7608-7610 UBERON:0002037 denotes Cb
T1638 7610-7614 PR:000005591 denotes Cln3
T1639 7639-7646 CHEBI:60004 denotes mixture
T1640 7650-7654 PR:000005591 denotes Cln3
T1641 7693-7699 SO:0001023 denotes allele
T1642 7715-7721 SO:0001023 denotes allele
T1643 9498-9500 UBERON:0002037 denotes Cb
T1644 9500-9504 PR:000005591 denotes Cln3
T1645 9547-9556 PR:000022190 denotes subunit c
T1646 9557-9566 GO:0016234 denotes inclusion
T1647 9557-9576 GO:0070841 denotes inclusion formation
T1648 9677-9679 UBERON:0002037 denotes Cb
T1649 9679-9683 PR:000005591 denotes Cln3
T1650 9699-9708 PR:000022190 denotes subunit c
T1651 9873-9882 PR:000022190 denotes subunit c
T1652 10041-10043 UBERON:0002037 denotes Cb
T1653 10043-10047 PR:000005591 denotes Cln3
T1654 10119-10127 CHEBI:10545 denotes electron
T1655 10134-10144 GO:0016234 denotes inclusions
T1656 10184-10198 GO:0005776 denotes autophagosomes
T1657 10214-10235 GO:0031045 denotes dense core structures
T1658 10250-10262 GO:0005739 denotes mitochondria
T1659 10281-10289 GO:0031982 denotes vesicles
T1660 10301-10317 GO:0016234 denotes Inclusion bodies
T1661 10322-10336 GO:0005776 denotes autophagosomes
T3117 15432-15434 UBERON:0002037 denotes Cb
T3118 15434-15438 PR:000005591 denotes Cln3
T3119 15465-15484 GO:0043227 denotes membrane organelles
T3120 15526-15537 PR:000006025 denotes cathepsin D
T3121 15548-15556 GO:0016020 denotes membrane
T3122 15548-15568 GO:0055085 denotes membrane trafficking
T3123 15595-15597 UBERON:0002037 denotes Cb
T3124 15597-15601 PR:000005591 denotes Cln3
T3125 15685-15704 GO:0043227 denotes membrane organelles
T3126 15757-15759 GO:0005783 denotes ER
T3127 15761-15770 GO:0005801 denotes cis-Golgi
T3173 18396-18405 GO:0005737 denotes cytoplasm
T3174 19108-19109 GO:0006897 denotes n
T3175 19110-19111 PR:000005591 denotes l
T3176 19115-19116 UBERON:0002037 denotes b
T3177 19124-19125 GO:0005634 denotes u
T3178 19143-19151 GO:0005739 denotes hondrial
T3179 19194-19231 GO:0000422 denotes autophagic engulfment of mitochondria
T3180 19219-19231 GO:0005739 denotes mitochondria
T3181 19261-19263 UBERON:0002037 denotes Cb
T3182 19263-19267 PR:000005591 denotes Cln3
T3183 19279-19292 GO:0005739 denotes mitochondrial
T3184 19318-19331 GO:0005739 denotes Mitochondrial
T3185 19359-19361 UBERON:0002037 denotes Cb
T3186 19361-19365 PR:000005591 denotes Cln3
T3187 19459-19461 UBERON:0002037 denotes Cb
T3188 19461-19465 PR:000005591 denotes Cln3
T3189 19472-19484 GO:0005739 denotes mitochondria
T3190 19512-19517 PR:000008813 denotes grp75
T3191 19593-19605 GO:0005739 denotes mitochondria
T4194 23129-23133 PR:000005591 denotes Cln3
T4195 23147-23158 PR:000006025 denotes cathepsin D
T4196 23225-23236 PR:000006025 denotes cathepsin D
T4197 23273-23282 PR:000022190 denotes subunit c
T4198 23305-23313 _FRAGMENT denotes Aging of
T4199 23338-23343 GO:0007569 denotes cells
T4200 23325-23327 UBERON:0002037 denotes Cb
T4201 23327-23331 PR:000005591 denotes Cln3
T4202 23407-23416 PR:000022190 denotes subunit c
T4203 23435-23453 GO:0043227 denotes membrane organelle
T4204 23474-23483 PR:000022190 denotes subunit c
T4205 23511-23513 UBERON:0002037 denotes Cb
T4206 23513-23517 PR:000005591 denotes Cln3
T4207 23563-23568 GO:0007568 denotes aging
T4208 23584-23593 GO:0005764 denotes Lysosomal
T4209 23598-23607 GO:0005768 denotes endosomal
T4210 23647-23659 GO:0005739 denotes mitochondria
T4211 23742-23744 UBERON:0002037 denotes Cb
T4212 23744-23748 PR:000005591 denotes Cln3
T4213 23795-23803 GO:0016020 denotes membrane
T4214 23795-23815 GO:0055085 denotes membrane trafficking
T4215 23853-23862 PR:000022190 denotes subunit c
T4216 23893-23901 GO:0005764 denotes lysosome
R2367 T4166 T4164 _lexicallyChainedTo trafficking,endosomal
R2368 T4169 T4168 _lexicallyChainedTo trafficking,lysosomal
R2369 T4199 T4198 _lexicallyChainedTo cells,Aging of
R2370 T4226 T4225 _lexicallyChainedTo of,transport
R2371 T4227 T4226 _lexicallyChainedTo proteins,of
R2372 T4235 T4234 _lexicallyChainedTo endosomes,early
R2373 T4258 T4257 _lexicallyChainedTo fusion,vesicular
R264 T540 T539 _lexicallyChainedTo mitochondrial ATP synthase complex,subunit c of
R2366 T4165 T4164 _lexicallyChainedTo membrane,endosomal
R1 T97 T96 _lexicallyChainedTo trafficking,intracellular
R6048 T9524 T9523 _lexicallyChainedTo cells,periphery of

craft-sa-dev

Id Subject Object Predicate Lexical cue
T170 0-8 NN denotes Membrane
T171 9-20 NN denotes trafficking
T172 0-151 sentence denotes Membrane trafficking and mitochondrial abnormalities precede subunit c deposition in a cerebellar cell model of juvenile neuronal ceroid lipofuscinosis
T173 53-60 VBP denotes precede
T174 21-24 CC denotes and
T175 25-38 JJ denotes mitochondrial
T176 39-52 NNS denotes abnormalities
T177 61-68 NN denotes subunit
T178 69-70 NN denotes c
T179 71-81 NN denotes deposition
T180 82-84 IN denotes in
T181 85-86 DT denotes a
T182 103-108 NN denotes model
T183 87-97 JJ denotes cerebellar
T184 98-102 NN denotes cell
T185 109-111 IN denotes of
T186 112-120 JJ denotes juvenile
T188 121-129 JJ denotes neuronal
T189 130-136 NN denotes ceroid
T190 151-152 sentence denotes
T191 162-172 sentence denotes Background
T192 162-172 NN denotes Background
T193 172-294 sentence denotes JNCL is a recessively inherited, childhood-onset neurodegenerative disease most-commonly caused by a ~1 kb CLN3 mutation.
T194 173-177 NN denotes JNCL
T195 178-180 VBZ denotes is
T196 181-182 DT denotes a
T197 240-247 NN denotes disease
T198 183-194 RB denotes recessively
T199 195-204 VBN denotes inherited
T200 204-206 , denotes ,
T201 206-215 NN denotes childhood
T202 216-221 NN denotes onset
T203 215-216 HYPH denotes -
T204 222-239 JJ denotes neurodegenerative
T205 248-252 RBS denotes most
T206 253-261 RB denotes commonly
T207 252-253 HYPH denotes -
T208 262-268 VBN denotes caused
T209 269-271 IN denotes by
T210 272-273 DT denotes a
T211 285-293 NN denotes mutation
T212 274-275 SYM denotes ~
T213 275-276 CD denotes 1
T214 277-279 NN denotes kb
T215 280-284 NN denotes CLN3
T216 293-294 . denotes .
T217 294-431 sentence denotes The resulting loss of battenin activity leads to deposition of mitochondrial ATP synthase, subunit c and a specific loss of CNS neurons.
T218 295-298 DT denotes The
T219 309-313 NN denotes loss
T220 299-308 JJ denotes resulting
T221 335-340 VBZ denotes leads
T222 314-316 IN denotes of
T223 317-325 NN denotes battenin
T224 326-334 NN denotes activity
T225 341-343 IN denotes to
T226 344-354 NN denotes deposition
T227 355-357 IN denotes of
T228 358-371 JJ denotes mitochondrial
T229 376-384 NN denotes synthase
T230 372-375 NN denotes ATP
T231 384-386 , denotes ,
T232 386-393 NN denotes subunit
T233 394-395 NN denotes c
T234 396-399 CC denotes and
T235 400-401 DT denotes a
T236 411-415 NN denotes loss
T237 402-410 JJ denotes specific
T238 416-418 IN denotes of
T239 419-422 NN denotes CNS
T240 423-430 NNS denotes neurons
T241 430-431 . denotes .
T242 431-580 sentence denotes We previously generated Cln3Δex7/8 knock-in mice, which replicate the common JNCL mutation, express mutant battenin and display JNCL-like pathology.
T243 432-434 PRP denotes We
T244 446-455 VBD denotes generated
T245 435-445 RB denotes previously
T246 456-464 NN denotes Cln3Δex7
T247 476-480 NNS denotes mice
T248 464-465 HYPH denotes /
T249 465-466 CD denotes 8
T250 467-472 VB denotes knock
T251 472-473 HYPH denotes -
T252 473-475 RP denotes in
T253 480-482 , denotes ,
T254 482-487 WDT denotes which
T255 488-497 VBP denotes replicate
T256 498-501 DT denotes the
T257 514-522 NN denotes mutation
T258 502-508 JJ denotes common
T259 509-513 NN denotes JNCL
T260 522-524 , denotes ,
T261 524-531 VBP denotes express
T262 532-538 NN denotes mutant
T263 539-547 NN denotes battenin
T264 548-551 CC denotes and
T265 552-559 VBP denotes display
T266 560-564 NN denotes JNCL
T267 565-569 JJ denotes like
T268 564-565 HYPH denotes -
T269 570-579 NN denotes pathology
T270 579-580 . denotes .
T271 580-589 sentence denotes Results
T272 582-589 NNS denotes Results
T273 589-897 sentence denotes To elucidate the consequences of the common JNCL mutation in neuronal cells, we used P4 knock-in mouse cerebella to establish conditionally immortalized CbCln3 wild-type, heterozygous, and homozygous neuronal precursor cell lines, which can be differentiated into MAP-2 and NeuN-positive, neuron-like cells.
T274 590-592 TO denotes To
T275 593-602 VB denotes elucidate
T276 670-674 VBD denotes used
T277 603-606 DT denotes the
T278 607-619 NNS denotes consequences
T279 620-622 IN denotes of
T280 623-626 DT denotes the
T281 639-647 NN denotes mutation
T282 627-633 JJ denotes common
T283 634-638 NN denotes JNCL
T284 648-650 IN denotes in
T285 651-659 JJ denotes neuronal
T286 660-665 NNS denotes cells
T287 665-667 , denotes ,
T288 667-669 PRP denotes we
T289 675-677 NN denotes P4
T290 693-702 NNS denotes cerebella
T291 678-683 VB denotes knock
T292 683-684 HYPH denotes -
T293 684-686 RP denotes in
T294 687-692 NN denotes mouse
T295 703-705 TO denotes to
T296 706-715 VB denotes establish
T297 716-729 RB denotes conditionally
T298 730-742 VBN denotes immortalized
T299 814-819 NNS denotes lines
T300 743-749 NN denotes CbCln3
T301 750-754 JJ denotes wild
T302 755-759 NN denotes type
T303 754-755 HYPH denotes -
T304 759-761 , denotes ,
T305 761-773 JJ denotes heterozygous
T306 773-775 , denotes ,
T307 775-778 CC denotes and
T308 779-789 JJ denotes homozygous
T309 790-798 JJ denotes neuronal
T310 799-808 NN denotes precursor
T311 809-813 NN denotes cell
T312 819-821 , denotes ,
T313 821-826 WDT denotes which
T314 834-848 VBN denotes differentiated
T315 827-830 MD denotes can
T316 831-833 VB denotes be
T317 849-853 IN denotes into
T318 854-857 NN denotes MAP
T319 869-877 JJ denotes positive
T320 857-858 HYPH denotes -
T321 858-859 CD denotes 2
T322 860-863 CC denotes and
T323 864-868 NN denotes NeuN
T324 868-869 HYPH denotes -
T325 891-896 NNS denotes cells
T326 877-879 , denotes ,
T327 879-885 NN denotes neuron
T328 886-890 JJ denotes like
T329 885-886 HYPH denotes -
T330 896-897 . denotes .
T331 897-1034 sentence denotes Homozygous CbCln3Δex7/8 precursor cells express low levels of mutant battenin and, when aged at confluency, accumulate ATPase subunit c.
T332 898-908 JJ denotes Homozygous
T333 932-937 NNS denotes cells
T334 909-919 NN denotes CbCln3Δex7
T335 919-920 HYPH denotes /
T336 920-921 CD denotes 8
T337 922-931 NN denotes precursor
T338 938-945 VBP denotes express
T339 946-949 JJ denotes low
T340 950-956 NNS denotes levels
T341 957-959 IN denotes of
T342 960-966 NN denotes mutant
T343 967-975 NN denotes battenin
T344 976-979 CC denotes and
T345 979-981 , denotes ,
T346 981-985 WRB denotes when
T347 986-990 VBN denotes aged
T348 1006-1016 VBP denotes accumulate
T349 991-993 IN denotes at
T350 994-1004 NN denotes confluency
T351 1004-1006 , denotes ,
T352 1017-1023 NN denotes ATPase
T353 1032-1033 NN denotes c
T354 1024-1031 NN denotes subunit
T355 1033-1034 . denotes .
T356 1034-1277 sentence denotes Recessive phenotypes are also observed at sub-confluent growth; cathepsin D transport and processing are altered, although enzyme activity is not significantly affected, lysosomal size and distribution are altered, and endocytosis is reduced.
T357 1035-1044 JJ denotes Recessive
T358 1045-1055 NNS denotes phenotypes
T359 1065-1073 VBN denotes observed
T360 1056-1059 VBP denotes are
T361 1060-1064 RB denotes also
T362 1074-1076 IN denotes at
T363 1077-1090 JJ denotes sub-confluent
T364 1091-1097 NN denotes growth
T365 1097-1098 : denotes ;
T366 1099-1108 NN denotes cathepsin
T367 1109-1110 NN denotes D
T368 1140-1147 VBN denotes altered
T369 1111-1120 NN denotes transport
T370 1121-1124 CC denotes and
T371 1125-1135 NN denotes processing
T372 1136-1139 VBP denotes are
T373 1147-1149 , denotes ,
T374 1149-1157 IN denotes although
T375 1195-1203 VBN denotes affected
T376 1158-1164 NN denotes enzyme
T377 1165-1173 NN denotes activity
T378 1174-1176 VBZ denotes is
T379 1177-1180 RB denotes not
T380 1181-1194 RB denotes significantly
T381 1241-1248 VBN denotes altered
T382 1203-1205 , denotes ,
T383 1205-1214 JJ denotes lysosomal
T384 1215-1219 NN denotes size
T385 1220-1223 CC denotes and
T386 1224-1236 NN denotes distribution
T387 1237-1240 VBP denotes are
T388 1248-1250 , denotes ,
T389 1250-1253 CC denotes and
T390 1254-1265 NN denotes endocytosis
T391 1269-1276 VBN denotes reduced
T392 1266-1268 VBZ denotes is
T393 1276-1277 . denotes .
T394 1277-1416 sentence denotes In addition, mitochondria are abnormally elongated, cellular ATP levels are decreased, and survival following oxidative stress is reduced.
T395 1278-1280 IN denotes In
T396 1304-1307 VBP denotes are
T397 1281-1289 NN denotes addition
T398 1289-1291 , denotes ,
T399 1291-1303 NNS denotes mitochondria
T400 1308-1318 RB denotes abnormally
T401 1319-1328 JJ denotes elongated
T402 1328-1330 , denotes ,
T403 1330-1338 JJ denotes cellular
T404 1343-1349 NNS denotes levels
T405 1339-1342 NN denotes ATP
T406 1354-1363 VBN denotes decreased
T407 1350-1353 VBP denotes are
T408 1363-1365 , denotes ,
T409 1365-1368 CC denotes and
T410 1369-1377 NN denotes survival
T411 1408-1415 VBN denotes reduced
T412 1378-1387 VBG denotes following
T413 1388-1397 JJ denotes oxidative
T414 1398-1404 NN denotes stress
T415 1405-1407 VBZ denotes is
T416 1415-1416 . denotes .
T417 1416-1429 sentence denotes Conclusions
T418 1418-1429 NNS denotes Conclusions
T419 1429-1544 sentence denotes These findings reveal that battenin is required for intracellular membrane trafficking and mitochondrial function.
T420 1430-1435 DT denotes These
T421 1436-1444 NNS denotes findings
T422 1445-1451 VBP denotes reveal
T423 1452-1456 IN denotes that
T424 1466-1468 VBZ denotes is
T425 1457-1465 NN denotes battenin
T426 1469-1477 JJ denotes required
T427 1478-1481 IN denotes for
T428 1482-1495 JJ denotes intracellular
T429 1505-1516 NN denotes trafficking
T430 1496-1504 NN denotes membrane
T431 1517-1520 CC denotes and
T432 1521-1534 JJ denotes mitochondrial
T433 1535-1543 NN denotes function
T434 1543-1544 . denotes .
T435 1544-1678 sentence denotes Moreover, these deficiencies are likely to be early events in the JNCL disease process and may particularly impact neuronal survival.
T436 1545-1553 RB denotes Moreover
T437 1574-1577 VBP denotes are
T438 1553-1555 , denotes ,
T439 1555-1560 DT denotes these
T440 1561-1573 NNS denotes deficiencies
T441 1578-1584 JJ denotes likely
T442 1585-1587 TO denotes to
T443 1588-1590 VB denotes be
T444 1591-1596 JJ denotes early
T445 1597-1603 NNS denotes events
T446 1604-1606 IN denotes in
T447 1607-1610 DT denotes the
T448 1624-1631 NN denotes process
T449 1611-1615 NN denotes JNCL
T450 1616-1623 NN denotes disease
T451 1632-1635 CC denotes and
T452 1636-1639 MD denotes may
T453 1653-1659 VB denotes impact
T454 1640-1652 RB denotes particularly
T455 1660-1668 JJ denotes neuronal
T456 1669-1677 NN denotes survival
T457 1677-1678 . denotes .
T696 1691-1699 JJ denotes Juvenile
T697 1716-1730 NN denotes lipofuscinosis
T698 1700-1708 JJ denotes neuronal
T699 1709-1715 NN denotes ceroid
T700 1758-1760 VBZ denotes is
T701 1731-1732 -LRB- denotes (
T702 1732-1736 NN denotes JNCL
T703 1736-1737 -RRB- denotes )
T704 1737-1739 , denotes ,
T705 1739-1741 CC denotes or
T706 1742-1748 NNP denotes Batten
T707 1749-1756 NN denotes disease
T708 1756-1758 , denotes ,
T709 1761-1762 DT denotes a
T710 1819-1827 NN denotes disorder
T711 1763-1774 RB denotes recessively
T712 1775-1784 VBN denotes inherited
T713 1785-1794 NN denotes childhood
T714 1794-1795 HYPH denotes -
T715 1795-1800 JJ denotes onset
T716 1801-1818 JJ denotes neurodegenerative
T717 1828-1841 VBN denotes characterized
T718 1842-1844 IN denotes by
T719 1845-1856 JJ denotes progressive
T720 1857-1866 NN denotes blindness
T721 1866-1868 , denotes ,
T722 1868-1876 NNS denotes seizures
T723 1876-1878 , denotes ,
T724 1878-1883 NN denotes motor
T725 1898-1905 NN denotes decline
T726 1884-1887 CC denotes and
T727 1888-1897 JJ denotes cognitive
T728 1905-1907 , denotes ,
T729 1907-1910 CC denotes and
T730 1911-1916 JJ denotes early
T731 1917-1922 NN denotes death
T732 1923-1924 -LRB- denotes [
T733 1924-1925 CD denotes 1
T734 1925-1926 -RRB- denotes ]
T735 1926-1927 . denotes .
T736 1927-2159 sentence denotes The primary genetic defect (>80% disease chromosomes) leading to JNCL is a 1.02 kb genomic DNA deletion in the CLN3 gene, which eliminates exons 7 and 8 and surrounding intronic DNA, predicting a non-functional protein product [2].
T737 1928-1931 DT denotes The
T738 1948-1954 NN denotes defect
T739 1932-1939 JJ denotes primary
T740 1940-1947 JJ denotes genetic
T741 1998-2000 VBZ denotes is
T742 1955-1956 -LRB- denotes (
T743 1969-1980 NNS denotes chromosomes
T744 1956-1957 SYM denotes >
T745 1957-1959 CD denotes 80
T746 1959-1960 NN denotes %
T747 1961-1968 NN denotes disease
T748 1980-1981 -RRB- denotes )
T749 1982-1989 VBG denotes leading
T750 1990-1992 IN denotes to
T751 1993-1997 NN denotes JNCL
T752 2001-2002 DT denotes a
T753 2023-2031 NN denotes deletion
T754 2003-2007 CD denotes 1.02
T755 2008-2010 NN denotes kb
T756 2011-2018 JJ denotes genomic
T757 2019-2022 NN denotes DNA
T758 2032-2034 IN denotes in
T759 2035-2038 DT denotes the
T760 2044-2048 NN denotes gene
T761 2039-2043 NN denotes CLN3
T762 2048-2050 , denotes ,
T763 2050-2055 WDT denotes which
T764 2056-2066 VBZ denotes eliminates
T765 2067-2072 NNS denotes exons
T766 2073-2074 CD denotes 7
T767 2075-2078 CC denotes and
T768 2079-2080 CD denotes 8
T769 2081-2084 CC denotes and
T770 2085-2096 JJ denotes surrounding
T771 2106-2109 NN denotes DNA
T772 2097-2105 JJ denotes intronic
T773 2109-2111 , denotes ,
T774 2111-2121 VBG denotes predicting
T775 2122-2123 DT denotes a
T776 2147-2154 NN denotes product
T777 2124-2138 JJ denotes non-functional
T778 2139-2146 NN denotes protein
T779 2155-2156 -LRB- denotes [
T780 2156-2157 CD denotes 2
T781 2157-2158 -RRB- denotes ]
T782 2158-2159 . denotes .
T783 2159-2340 sentence denotes The pathological hallmark of JNCL is autofluorescent ceroid lipofuscin deposits within autolysosomes that are enriched in subunit c of the mitochondrial ATP synthase complex [3-5].
T784 2160-2163 DT denotes The
T785 2177-2185 NN denotes hallmark
T786 2164-2176 JJ denotes pathological
T787 2194-2196 VBZ denotes is
T788 2186-2188 IN denotes of
T789 2189-2193 NN denotes JNCL
T790 2197-2212 JJ denotes autofluorescent
T791 2231-2239 NNS denotes deposits
T792 2213-2219 NN denotes ceroid
T793 2220-2230 NN denotes lipofuscin
T794 2240-2246 IN denotes within
T795 2247-2260 NNS denotes autolysosomes
T796 2261-2265 WDT denotes that
T797 2266-2269 VBP denotes are
T798 2270-2278 JJ denotes enriched
T799 2279-2281 IN denotes in
T800 2282-2289 NN denotes subunit
T801 2290-2291 NN denotes c
T802 2292-2294 IN denotes of
T803 2295-2298 DT denotes the
T804 2326-2333 NN denotes complex
T805 2299-2312 JJ denotes mitochondrial
T806 2317-2325 NN denotes synthase
T807 2313-2316 NN denotes ATP
T808 2334-2335 -LRB- denotes [
T809 2335-2336 CD denotes 3
T810 2336-2337 SYM denotes -
T811 2337-2338 CD denotes 5
T812 2338-2339 -RRB- denotes ]
T813 2339-2340 . denotes .
T814 2340-2476 sentence denotes Remarkably, these deposits are not only found in CNS neurons but are also abundant in non-neuronal cells outside of the nervous system.
T815 2341-2351 RB denotes Remarkably
T816 2381-2386 VBN denotes found
T817 2351-2353 , denotes ,
T818 2353-2358 DT denotes these
T819 2359-2367 NNS denotes deposits
T820 2368-2371 VBP denotes are
T821 2372-2375 RB denotes not
T822 2376-2380 RB denotes only
T823 2387-2389 IN denotes in
T824 2390-2393 NN denotes CNS
T825 2394-2401 NNS denotes neurons
T826 2402-2405 CC denotes but
T827 2406-2409 VBP denotes are
T828 2410-2414 RB denotes also
T829 2415-2423 JJ denotes abundant
T830 2424-2426 IN denotes in
T831 2427-2439 JJ denotes non-neuronal
T832 2440-2445 NNS denotes cells
T833 2446-2453 IN denotes outside
T834 2454-2456 IN denotes of
T835 2457-2460 DT denotes the
T836 2469-2475 NN denotes system
T837 2461-2468 JJ denotes nervous
T838 2475-2476 . denotes .
T839 2476-2640 sentence denotes The relationship of subunit c deposits to the JNCL disease process, and the underlying reason for the neuronal specificity of the disease remain poorly understood.
T840 2477-2480 DT denotes The
T841 2481-2493 NN denotes relationship
T842 2615-2621 VBP denotes remain
T843 2494-2496 IN denotes of
T844 2497-2504 NN denotes subunit
T845 2505-2506 NN denotes c
T846 2507-2515 NNS denotes deposits
T847 2516-2518 IN denotes to
T848 2519-2522 DT denotes the
T849 2536-2543 NN denotes process
T850 2523-2527 NN denotes JNCL
T851 2528-2535 NN denotes disease
T852 2543-2545 , denotes ,
T853 2545-2548 CC denotes and
T854 2549-2552 DT denotes the
T855 2564-2570 NN denotes reason
T856 2553-2563 JJ denotes underlying
T857 2571-2574 IN denotes for
T858 2575-2578 DT denotes the
T859 2588-2599 NN denotes specificity
T860 2579-2587 JJ denotes neuronal
T861 2600-2602 IN denotes of
T862 2603-2606 DT denotes the
T863 2607-2614 NN denotes disease
T864 2622-2628 RB denotes poorly
T865 2629-2639 VBN denotes understood
T866 2639-2640 . denotes .
T867 2640-2854 sentence denotes The CLN3-encoded protein (battenin, also called CLN3 or cln3 p) is a highly conserved, ubiquitously expressed, multi-pass membrane protein [6] that localizes to the lysosome and other vesicular compartments [7-9].
T868 2641-2644 DT denotes The
T869 2658-2665 NN denotes protein
T870 2645-2649 NN denotes CLN3
T871 2650-2657 VBN denotes encoded
T872 2649-2650 HYPH denotes -
T873 2705-2707 VBZ denotes is
T874 2666-2667 -LRB- denotes (
T875 2667-2675 NN denotes battenin
T876 2675-2677 , denotes ,
T877 2677-2681 RB denotes also
T878 2682-2688 VBN denotes called
T879 2689-2693 NN denotes CLN3
T880 2694-2696 CC denotes or
T881 2697-2701 NN denotes cln3
T882 2702-2703 NN denotes p
T883 2703-2704 -RRB- denotes )
T884 2708-2709 DT denotes a
T885 2772-2779 NN denotes protein
T886 2710-2716 RB denotes highly
T887 2717-2726 JJ denotes conserved
T888 2726-2728 , denotes ,
T889 2728-2740 RB denotes ubiquitously
T890 2741-2750 VBN denotes expressed
T891 2750-2752 , denotes ,
T892 2752-2762 NN denotes multi-pass
T893 2763-2771 NN denotes membrane
T894 2780-2781 -LRB- denotes [
T895 2781-2782 CD denotes 6
T896 2782-2783 -RRB- denotes ]
T897 2784-2788 WDT denotes that
T898 2789-2798 VBZ denotes localizes
T899 2799-2801 IN denotes to
T900 2802-2805 DT denotes the
T901 2806-2814 NN denotes lysosome
T902 2815-2818 CC denotes and
T903 2819-2824 JJ denotes other
T904 2835-2847 NNS denotes compartments
T905 2825-2834 JJ denotes vesicular
T906 2848-2849 -LRB- denotes [
T907 2849-2850 CD denotes 7
T908 2850-2851 SYM denotes -
T909 2851-2852 CD denotes 9
T910 2852-2853 -RRB- denotes ]
T911 2853-2854 . denotes .
T912 2854-3036 sentence denotes Battenin function remains to be elucidated, although studies of btn1, the yeast CLN3 ortholog, have implicated battenin in lysosomal pH homeostasis and amino acid transport [10,11].
T913 2855-2863 NN denotes Battenin
T914 2864-2872 NN denotes function
T915 2873-2880 VBZ denotes remains
T916 2881-2883 TO denotes to
T917 2887-2897 VBN denotes elucidated
T918 2884-2886 VB denotes be
T919 2897-2899 , denotes ,
T920 2899-2907 IN denotes although
T921 2955-2965 VBN denotes implicated
T922 2908-2915 NNS denotes studies
T923 2916-2918 IN denotes of
T924 2919-2923 NN denotes btn1
T925 2923-2925 , denotes ,
T926 2925-2928 DT denotes the
T927 2940-2948 NN denotes ortholog
T928 2929-2934 NN denotes yeast
T929 2935-2939 NN denotes CLN3
T930 2948-2950 , denotes ,
T931 2950-2954 VBP denotes have
T932 2966-2974 NN denotes battenin
T933 2975-2977 IN denotes in
T934 2978-2987 JJ denotes lysosomal
T935 2991-3002 NN denotes homeostasis
T936 2988-2990 NN denotes pH
T937 3003-3006 CC denotes and
T938 3007-3012 NN denotes amino
T939 3013-3017 NN denotes acid
T940 3018-3027 NN denotes transport
T941 3028-3029 -LRB- denotes [
T942 3032-3034 CD denotes 11
T943 3029-3031 CD denotes 10
T944 3031-3032 , denotes ,
T945 3034-3035 -RRB- denotes ]
T946 3035-3036 . denotes .
T947 3036-3152 sentence denotes To explore JNCL pathogenesis and battenin function, we previously generated a genetically precise JNCL mouse model.
T948 3037-3039 TO denotes To
T949 3040-3047 VB denotes explore
T950 3103-3112 VBD denotes generated
T951 3048-3052 NN denotes JNCL
T952 3053-3065 NN denotes pathogenesis
T953 3066-3069 CC denotes and
T954 3070-3078 NN denotes battenin
T955 3079-3087 NN denotes function
T956 3087-3089 , denotes ,
T957 3089-3091 PRP denotes we
T958 3092-3102 RB denotes previously
T959 3113-3114 DT denotes a
T960 3146-3151 NN denotes model
T961 3115-3126 RB denotes genetically
T962 3127-3134 JJ denotes precise
T963 3135-3139 NN denotes JNCL
T964 3140-3145 NN denotes mouse
T965 3151-3152 . denotes .
T966 3152-3335 sentence denotes Cln3Δex7/8 knock-in mice harbor the ~1 kb common JNCL mutation and express a non-truncated mutant battenin isoform that is detectable with antibodies recognizing C-terminal epitopes.
T967 3153-3161 NN denotes Cln3Δex7
T968 3173-3177 NNS denotes mice
T969 3161-3162 HYPH denotes /
T970 3162-3163 CD denotes 8
T971 3164-3169 VB denotes knock
T972 3169-3170 HYPH denotes -
T973 3170-3172 RP denotes in
T974 3178-3184 VBP denotes harbor
T975 3185-3188 DT denotes the
T976 3207-3215 NN denotes mutation
T977 3189-3190 SYM denotes ~
T978 3190-3191 CD denotes 1
T979 3192-3194 NN denotes kb
T980 3195-3201 JJ denotes common
T981 3202-3206 NN denotes JNCL
T982 3216-3219 CC denotes and
T983 3220-3227 VBP denotes express
T984 3228-3229 DT denotes a
T985 3260-3267 NN denotes isoform
T986 3230-3243 JJ denotes non-truncated
T987 3244-3250 NN denotes mutant
T988 3251-3259 NN denotes battenin
T989 3268-3272 WDT denotes that
T990 3273-3275 VBZ denotes is
T991 3276-3286 JJ denotes detectable
T992 3287-3291 IN denotes with
T993 3292-3302 NNS denotes antibodies
T994 3303-3314 VBG denotes recognizing
T995 3315-3316 NN denotes C
T996 3317-3325 JJ denotes terminal
T997 3316-3317 HYPH denotes -
T998 3326-3334 NNS denotes epitopes
T999 3334-3335 . denotes .
T1000 3335-3546 sentence denotes Homozygous Cln3Δex7/8 knock-in mice exhibit a progressive JNCL-like disease, with perinatal onset of subunit c deposition in many cell types and later onset of neuronal dysfunction and behavioral deficits [12].
T1001 3336-3346 JJ denotes Homozygous
T1002 3367-3371 NNS denotes mice
T1003 3347-3355 NN denotes Cln3Δex7
T1004 3355-3356 HYPH denotes /
T1005 3356-3357 CD denotes 8
T1006 3358-3363 VB denotes knock
T1007 3363-3364 HYPH denotes -
T1008 3364-3366 RP denotes in
T1009 3372-3379 VBP denotes exhibit
T1010 3380-3381 DT denotes a
T1011 3404-3411 NN denotes disease
T1012 3382-3393 JJ denotes progressive
T1013 3394-3398 NN denotes JNCL
T1014 3399-3403 JJ denotes like
T1015 3398-3399 HYPH denotes -
T1016 3411-3413 , denotes ,
T1017 3413-3417 IN denotes with
T1018 3418-3427 JJ denotes perinatal
T1019 3428-3433 NN denotes onset
T1020 3434-3436 IN denotes of
T1021 3437-3444 NN denotes subunit
T1022 3445-3446 NN denotes c
T1023 3447-3457 NN denotes deposition
T1024 3458-3460 IN denotes in
T1025 3461-3465 JJ denotes many
T1026 3471-3476 NNS denotes types
T1027 3466-3470 NN denotes cell
T1028 3477-3480 CC denotes and
T1029 3481-3486 JJ denotes later
T1030 3487-3492 NN denotes onset
T1031 3493-3495 IN denotes of
T1032 3496-3504 JJ denotes neuronal
T1033 3505-3516 NN denotes dysfunction
T1034 3517-3520 CC denotes and
T1035 3521-3531 JJ denotes behavioral
T1061 3657-3663 NN denotes manner
T1036 3532-3540 NNS denotes deficits
T1037 3541-3542 -LRB- denotes [
T1038 3542-3544 CD denotes 12
T1039 3544-3545 -RRB- denotes ]
T1040 3545-3546 . denotes .
T1041 3546-3705 sentence denotes These findings suggest that the major JNCL defect leads to abnormal turnover of mitochondrial subunit c, in a manner that selectively compromises CNS neurons.
T1042 3547-3552 DT denotes These
T1043 3553-3561 NNS denotes findings
T1044 3562-3569 VBP denotes suggest
T1045 3570-3574 IN denotes that
T1046 3597-3602 VBZ denotes leads
T1047 3575-3578 DT denotes the
T1048 3590-3596 NN denotes defect
T1049 3579-3584 JJ denotes major
T1050 3585-3589 NN denotes JNCL
T1051 3603-3605 IN denotes to
T1052 3606-3614 JJ denotes abnormal
T1053 3615-3623 NN denotes turnover
T1054 3624-3626 IN denotes of
T1055 3627-3640 JJ denotes mitochondrial
T1056 3649-3650 NN denotes c
T1057 3641-3648 NN denotes subunit
T1058 3650-3652 , denotes ,
T1059 3652-3654 IN denotes in
T1060 3655-3656 DT denotes a
T1062 3664-3668 WDT denotes that
T1063 3681-3692 VBZ denotes compromises
T1064 3669-3680 RB denotes selectively
T1065 3693-3696 NN denotes CNS
T1066 3697-3704 NNS denotes neurons
T1067 3704-3705 . denotes .
T1068 3705-3837 sentence denotes Currently, there is no suitable neuronal cell system to investigate the impact of the common JNCL mutation on biological processes.
T1069 3706-3715 RB denotes Currently
T1070 3723-3725 VBZ denotes is
T1071 3715-3717 , denotes ,
T1072 3717-3722 EX denotes there
T1073 3726-3728 DT denotes no
T1074 3752-3758 NN denotes system
T1075 3729-3737 JJ denotes suitable
T1076 3738-3746 JJ denotes neuronal
T1077 3747-3751 NN denotes cell
T1078 3759-3761 TO denotes to
T1079 3762-3773 VB denotes investigate
T1080 3774-3777 DT denotes the
T1081 3778-3784 NN denotes impact
T1082 3785-3787 IN denotes of
T1083 3788-3791 DT denotes the
T1084 3804-3812 NN denotes mutation
T1085 3792-3798 JJ denotes common
T1086 3799-3803 NN denotes JNCL
T1087 3813-3815 IN denotes on
T1088 3816-3826 JJ denotes biological
T1089 3827-3836 NNS denotes processes
T1090 3836-3837 . denotes .
T1091 3837-3940 sentence denotes Therefore, we have established cerebellar neuronal precursor cell lines from Cln3Δex7/8 knock-in mice.
T1092 3838-3847 RB denotes Therefore
T1093 3857-3868 VBN denotes established
T1094 3847-3849 , denotes ,
T1095 3849-3851 PRP denotes we
T1096 3852-3856 VBP denotes have
T1097 3869-3879 JJ denotes cerebellar
T1098 3904-3909 NNS denotes lines
T1099 3880-3888 JJ denotes neuronal
T1100 3889-3898 NN denotes precursor
T1101 3899-3903 NN denotes cell
T1102 3910-3914 IN denotes from
T1103 3915-3923 NN denotes Cln3Δex7
T1104 3935-3939 NNS denotes mice
T1105 3923-3924 HYPH denotes /
T1106 3924-3925 CD denotes 8
T1107 3926-3931 VB denotes knock
T1108 3931-3932 HYPH denotes -
T1109 3932-3934 RP denotes in
T1110 3939-3940 . denotes .
T1111 3940-4162 sentence denotes Homozygous CbCln3Δex7/8 cells exhibit pathological hallmarks of the disease, and a survey of membrane organelles revealed membrane trafficking defects and mitochondrial dysfunction in homozygous mutant CbCln3Δex7/8 cells.
T1112 3941-3951 JJ denotes Homozygous
T1113 3965-3970 NNS denotes cells
T1114 3952-3962 NN denotes CbCln3Δex7
T1115 3962-3963 HYPH denotes /
T1116 3963-3964 CD denotes 8
T1117 3971-3978 VBP denotes exhibit
T1118 3979-3991 JJ denotes pathological
T1119 3992-4001 NNS denotes hallmarks
T1120 4002-4004 IN denotes of
T1121 4005-4008 DT denotes the
T1122 4009-4016 NN denotes disease
T1123 4016-4018 , denotes ,
T1124 4018-4021 CC denotes and
T1125 4022-4023 DT denotes a
T1126 4024-4030 NN denotes survey
T1127 4054-4062 VBD denotes revealed
T1128 4031-4033 IN denotes of
T1129 4034-4042 NN denotes membrane
T1130 4043-4053 NNS denotes organelles
T1131 4063-4071 NN denotes membrane
T1132 4072-4083 VBG denotes trafficking
T1133 4084-4091 NNS denotes defects
T1134 4092-4095 CC denotes and
T1135 4096-4109 JJ denotes mitochondrial
T1136 4110-4121 NN denotes dysfunction
T1137 4122-4124 IN denotes in
T1138 4125-4135 JJ denotes homozygous
T1139 4136-4142 NN denotes mutant
T1140 4156-4161 NNS denotes cells
T1141 4143-4153 NN denotes CbCln3Δex7
T1142 4153-4154 HYPH denotes /
T1143 4154-4155 CD denotes 8
T1144 4161-4162 . denotes .
T1271 4173-4183 NN denotes Generation
T1272 4184-4186 IN denotes of
T1273 4187-4188 DT denotes a
T1274 4230-4235 NN denotes model
T1275 4189-4200 RB denotes genetically
T1276 4201-4208 JJ denotes precise
T1277 4209-4219 JJ denotes cerebellar
T1278 4220-4224 NN denotes JNCL
T1279 4225-4229 NN denotes cell
T1280 4235-4468 sentence denotes To generate a precise genetic, neuron-derived JNCL cell culture system, we immortalized granule neurons cultured from postnatal day 4 (P4) cerebella of homozygous and heterozygous Cln3Δex7/8 knock-in mice, and wild-type littermates.
T1281 4236-4238 TO denotes To
T1282 4239-4247 VB denotes generate
T1283 4311-4323 VBD denotes immortalized
T1284 4248-4249 DT denotes a
T1285 4300-4306 NN denotes system
T1286 4250-4257 JJ denotes precise
T1287 4258-4265 JJ denotes genetic
T1288 4265-4267 , denotes ,
T1289 4267-4273 NN denotes neuron
T1290 4274-4281 VBN denotes derived
T1291 4273-4274 HYPH denotes -
T1292 4282-4286 NN denotes JNCL
T1293 4292-4299 NN denotes culture
T1294 4287-4291 NN denotes cell
T1295 4306-4308 , denotes ,
T1296 4308-4310 PRP denotes we
T1297 4324-4331 NN denotes granule
T1298 4332-4339 NNS denotes neurons
T1299 4340-4348 VBN denotes cultured
T1300 4349-4353 IN denotes from
T1301 4354-4363 JJ denotes postnatal
T1302 4364-4367 NN denotes day
T1303 4375-4384 NNS denotes cerebella
T1304 4368-4369 CD denotes 4
T1305 4370-4371 -LRB- denotes (
T1306 4371-4373 NN denotes P4
T1307 4373-4374 -RRB- denotes )
T1308 4385-4387 IN denotes of
T1309 4388-4398 JJ denotes homozygous
T1310 4436-4440 NNS denotes mice
T1311 4399-4402 CC denotes and
T1312 4403-4415 JJ denotes heterozygous
T1313 4416-4424 NN denotes Cln3Δex7
T1314 4424-4425 HYPH denotes /
T1315 4425-4426 CD denotes 8
T1316 4427-4432 VB denotes knock
T1317 4432-4433 HYPH denotes -
T1318 4433-4435 RP denotes in
T1319 4440-4442 , denotes ,
T1320 4442-4445 CC denotes and
T1321 4446-4450 JJ denotes wild
T1322 4451-4455 NN denotes type
T1323 4450-4451 HYPH denotes -
T1324 4456-4467 NNS denotes littermates
T1325 4467-4468 . denotes .
T1326 4468-4641 sentence denotes Primary cell cultures enriched for granule neurons were transduced with retroviral vector bearing a selection cassette and temperature-sensitive tsA58 SV40 large T antigen.
T1327 4469-4476 JJ denotes Primary
T1328 4482-4490 NNS denotes cultures
T1329 4477-4481 NN denotes cell
T1330 4525-4535 VBN denotes transduced
T1331 4491-4499 VBN denotes enriched
T1332 4500-4503 IN denotes for
T1333 4504-4511 NN denotes granule
T1334 4512-4519 NNS denotes neurons
T1335 4520-4524 VBD denotes were
T1336 4536-4540 IN denotes with
T1337 4541-4551 JJ denotes retroviral
T1338 4552-4558 NN denotes vector
T1339 4559-4566 VBG denotes bearing
T1340 4567-4568 DT denotes a
T1341 4579-4587 NN denotes cassette
T1342 4569-4578 NN denotes selection
T1343 4588-4591 CC denotes and
T1344 4592-4603 NN denotes temperature
T1345 4604-4613 JJ denotes sensitive
T1346 4603-4604 HYPH denotes -
T1347 4633-4640 NN denotes antigen
T1348 4614-4619 NN denotes tsA58
T1349 4620-4624 NN denotes SV40
T1350 4625-4630 JJ denotes large
T1351 4631-4632 NN denotes T
T1352 4640-4641 . denotes .
T1353 4641-4852 sentence denotes Growth in G418 containing medium at the permissive temperature (33°C) allowed for selection and isolation of multiple clonal nestin-positive (Fig. 1a), and GFAP-negative (Fig. 1b), cell lines for each genotype.
T1354 4642-4648 NN denotes Growth
T1355 4712-4719 VBN denotes allowed
T1356 4649-4651 IN denotes in
T1357 4652-4656 NN denotes G418
T1358 4657-4667 VBG denotes containing
T1359 4668-4674 NN denotes medium
T1360 4675-4677 IN denotes at
T1361 4678-4681 DT denotes the
T1362 4693-4704 NN denotes temperature
T1363 4682-4692 JJ denotes permissive
T1364 4705-4706 -LRB- denotes (
T1365 4706-4708 CD denotes 33
T1366 4708-4710 NN denotes °C
T1367 4710-4711 -RRB- denotes )
T1368 4720-4723 IN denotes for
T1369 4724-4733 NN denotes selection
T1370 4734-4737 CC denotes and
T1371 4738-4747 NN denotes isolation
T1372 4748-4750 IN denotes of
T1373 4751-4759 JJ denotes multiple
T1374 4828-4833 NNS denotes lines
T1375 4760-4766 JJ denotes clonal
T1376 4767-4773 NN denotes nestin
T1377 4774-4782 JJ denotes positive
T1378 4773-4774 HYPH denotes -
T1379 4783-4784 -LRB- denotes (
T1380 4789-4791 NN denotes 1a
T1381 4784-4788 NN denotes Fig.
T1382 4791-4792 -RRB- denotes )
T1383 4792-4794 , denotes ,
T1384 4794-4797 CC denotes and
T1385 4798-4802 NN denotes GFAP
T1386 4803-4811 JJ denotes negative
T1387 4802-4803 HYPH denotes -
T1388 4812-4813 -LRB- denotes (
T1389 4818-4820 NN denotes 1b
T1390 4813-4817 NN denotes Fig.
T1391 4820-4821 -RRB- denotes )
T1392 4821-4823 , denotes ,
T1393 4823-4827 NN denotes cell
T1394 4834-4837 IN denotes for
T1395 4838-4842 DT denotes each
T1396 4843-4851 NN denotes genotype
T1397 4851-4852 . denotes .
T1398 4852-4969 sentence denotes No genotype specific differences were observed in cellular morphology or doubling time (~46 hours) (data not shown).
T1399 4853-4855 DT denotes No
T1400 4874-4885 NNS denotes differences
T1401 4856-4864 NN denotes genotype
T1402 4865-4873 JJ denotes specific
T1403 4891-4899 VBN denotes observed
T1404 4886-4890 VBD denotes were
T1405 4900-4902 IN denotes in
T1406 4903-4911 JJ denotes cellular
T1407 4912-4922 NN denotes morphology
T1408 4923-4925 CC denotes or
T1409 4926-4934 NN denotes doubling
T1410 4935-4939 NN denotes time
T1411 4940-4941 -LRB- denotes (
T1412 4941-4942 SYM denotes ~
T1413 4942-4944 CD denotes 46
T1414 4945-4950 NNS denotes hours
T1415 4950-4951 -RRB- denotes )
T1416 4952-4953 -LRB- denotes (
T1417 4962-4967 VBN denotes shown
T1418 4953-4957 NNS denotes data
T1419 4958-4961 RB denotes not
T1420 4967-4968 -RRB- denotes )
T1421 4968-4969 . denotes .
T1422 4969-5139 sentence denotes As expected, SV40 large T antigen expression was rapidly lost and cell division ceased when cells were shifted to the non-permissive temperature (39°C) (data not shown).
T1423 4970-4972 IN denotes As
T1424 4973-4981 VBN denotes expected
T1425 5027-5031 VBN denotes lost
T1426 4981-4983 , denotes ,
T1427 4983-4987 NN denotes SV40
T1428 5004-5014 NN denotes expression
T1429 4988-4993 JJ denotes large
T1430 4994-4995 NN denotes T
T1431 4996-5003 NN denotes antigen
T1432 5015-5018 VBD denotes was
T1433 5019-5026 RB denotes rapidly
T1434 5032-5035 CC denotes and
T1435 5036-5040 NN denotes cell
T1436 5041-5049 NN denotes division
T1437 5050-5056 VBD denotes ceased
T1438 5057-5061 WRB denotes when
T1439 5073-5080 VBN denotes shifted
T1440 5062-5067 NNS denotes cells
T1441 5068-5072 VBD denotes were
T1442 5081-5083 IN denotes to
T1443 5084-5087 DT denotes the
T1444 5103-5114 NN denotes temperature
T1445 5088-5102 JJ denotes non-permissive
T1446 5115-5116 -LRB- denotes (
T1447 5116-5118 CD denotes 39
T1448 5118-5120 NN denotes °C
T1449 5120-5121 -RRB- denotes )
T1450 5122-5123 -LRB- denotes (
T1451 5132-5137 VBN denotes shown
T1452 5123-5127 NNS denotes data
T1453 5128-5131 RB denotes not
T1454 5137-5138 -RRB- denotes )
T1455 5138-5139 . denotes .
T1456 5139-5416 sentence denotes Upon addition of neuronal differentiation cocktail, precursor cells became neuron-like in morphology and exhibited decreased nestin expression (data not shown) and increased MAP2 and NeuN expression (Fig. 1c,1d), but not expression of the Purkinje marker, calbindin (Fig. 1e).
T1457 5140-5144 IN denotes Upon
T1458 5208-5214 VBD denotes became
T1459 5145-5153 NN denotes addition
T1460 5154-5156 IN denotes of
T1461 5157-5165 JJ denotes neuronal
T1462 5166-5181 NN denotes differentiation
T1463 5182-5190 NN denotes cocktail
T1464 5190-5192 , denotes ,
T1465 5192-5201 NN denotes precursor
T1466 5202-5207 NNS denotes cells
T1467 5215-5221 NN denotes neuron
T1468 5222-5226 JJ denotes like
T1469 5221-5222 HYPH denotes -
T1470 5227-5229 IN denotes in
T1471 5230-5240 NN denotes morphology
T1472 5241-5244 CC denotes and
T1473 5245-5254 VBD denotes exhibited
T1474 5255-5264 VBN denotes decreased
T1475 5272-5282 NN denotes expression
T1476 5265-5271 NN denotes nestin
T1477 5283-5284 -LRB- denotes (
T1478 5293-5298 VBN denotes shown
T1479 5284-5288 NNS denotes data
T1480 5289-5292 RB denotes not
T1481 5298-5299 -RRB- denotes )
T1482 5300-5303 CC denotes and
T1483 5304-5313 VBN denotes increased
T1484 5328-5338 NN denotes expression
T1485 5314-5318 NN denotes MAP2
T1486 5319-5322 CC denotes and
T1487 5323-5327 NN denotes NeuN
T1488 5339-5340 -LRB- denotes (
T1489 5348-5350 NN denotes 1d
T1490 5340-5344 NN denotes Fig.
T1491 5345-5347 NN denotes 1c
T1492 5347-5348 , denotes ,
T1493 5350-5351 -RRB- denotes )
T1494 5351-5353 , denotes ,
T1495 5353-5356 CC denotes but
T1496 5357-5360 RB denotes not
T1497 5361-5371 NN denotes expression
T1498 5372-5374 IN denotes of
T1499 5375-5378 DT denotes the
T1500 5388-5394 NN denotes marker
T1501 5379-5387 NNP denotes Purkinje
T1502 5394-5396 , denotes ,
T1503 5396-5405 NN denotes calbindin
T1504 5406-5407 -LRB- denotes (
T1505 5412-5414 NN denotes 1e
T1506 5407-5411 NN denotes Fig.
T1507 5414-5415 -RRB- denotes )
T1508 5415-5416 . denotes .
T1775 6145-6155 JJ denotes Homozygous
T1776 6169-6174 NNS denotes cells
T1777 6156-6166 NN denotes CbCln3Δex7
T1778 6166-6167 HYPH denotes /
T1779 6167-6168 CD denotes 8
T1780 6175-6182 VBP denotes express
T1781 6183-6189 NN denotes mutant
T1782 6190-6198 NN denotes battenin
T1783 6199-6202 CC denotes and
T1784 6203-6210 VBP denotes display
T1785 6211-6215 NN denotes JNCL
T1786 6216-6220 JJ denotes like
T1787 6215-6216 HYPH denotes -
T1788 6221-6230 NN denotes pathology
T1789 6230-6312 sentence denotes Homozygous Cb Cln3Δex7/8 cells were first examined for JNCL-like characteristics.
T1790 6231-6241 JJ denotes Homozygous
T1791 6256-6261 NNS denotes cells
T1792 6242-6244 NN denotes Cb
T1793 6245-6253 NN denotes Cln3Δex7
T1794 6253-6254 HYPH denotes /
T1795 6254-6255 CD denotes 8
T1796 6273-6281 VBN denotes examined
T1797 6262-6266 VBD denotes were
T1798 6267-6272 RB denotes first
T1799 6282-6285 IN denotes for
T1800 6286-6290 NN denotes JNCL
T1801 6291-6295 JJ denotes like
T1802 6290-6291 HYPH denotes -
T1803 6296-6311 NNS denotes characteristics
T1804 6311-6312 . denotes .
T1805 6312-6494 sentence denotes Homozygous Cln3Δex7/8 knock-in mice express multiple Cln3 mRNA splice variants and mutant battenin protein that is detectable by batp1 antibody recognizing C-terminal epitopes [12].
T1806 6313-6323 JJ denotes Homozygous
T1807 6344-6348 NNS denotes mice
T1808 6324-6332 NN denotes Cln3Δex7
T1809 6332-6333 HYPH denotes /
T1810 6333-6334 CD denotes 8
T1811 6335-6340 VB denotes knock
T1812 6340-6341 HYPH denotes -
T1813 6341-6343 RP denotes in
T1814 6349-6356 VBP denotes express
T1815 6357-6365 JJ denotes multiple
T1816 6383-6391 NNS denotes variants
T1817 6366-6370 NN denotes Cln3
T1818 6376-6382 NN denotes splice
T1819 6371-6375 NN denotes mRNA
T1820 6392-6395 CC denotes and
T1821 6396-6402 NN denotes mutant
T1822 6412-6419 NN denotes protein
T1823 6403-6411 NN denotes battenin
T1824 6420-6424 WDT denotes that
T1825 6425-6427 VBZ denotes is
T1826 6428-6438 JJ denotes detectable
T1827 6439-6441 IN denotes by
T1828 6442-6447 NN denotes batp1
T1829 6448-6456 NN denotes antibody
T1830 6457-6468 VBG denotes recognizing
T1831 6480-6488 NNS denotes epitopes
T1832 6469-6470 NN denotes C
T1833 6471-6479 JJ denotes terminal
T1834 6470-6471 HYPH denotes -
T1835 6489-6490 -LRB- denotes [
T1836 6490-6492 CD denotes 12
T1837 6492-6493 -RRB- denotes ]
T1838 6493-6494 . denotes .
T1839 6494-6616 sentence denotes To assess this molecular phenotype in CbCln3Δex7/8 cells, RT-PCR and anti-battenin (batp1) immunostaining were performed.
T1840 6495-6497 TO denotes To
T1841 6498-6504 VB denotes assess
T1842 6606-6615 VBN denotes performed
T1843 6505-6509 DT denotes this
T1844 6520-6529 NN denotes phenotype
T1845 6510-6519 JJ denotes molecular
T1846 6530-6532 IN denotes in
T1847 6533-6543 NN denotes CbCln3Δex7
T1848 6546-6551 NNS denotes cells
T1849 6543-6544 HYPH denotes /
T1850 6544-6545 CD denotes 8
T1851 6551-6553 , denotes ,
T1852 6553-6555 NN denotes RT
T1853 6556-6559 NN denotes PCR
T1854 6555-6556 HYPH denotes -
T1855 6560-6563 CC denotes and
T1856 6564-6577 JJ denotes anti-battenin
T1857 6586-6600 NN denotes immunostaining
T1858 6578-6579 -LRB- denotes (
T1859 6579-6584 NN denotes batp1
T1860 6584-6585 -RRB- denotes )
T1861 6601-6605 VBD denotes were
T1862 6615-6616 . denotes .
T1863 6616-6823 sentence denotes As shown in Figure 2, Cln3 mRNA isoforms in wild-type and homozygous cells were similar to those observed in total RNA isolated from wild-type or homozygous Cln3Δex7/8 knock-in brain, respectively (Fig. 2).
T1864 6617-6619 IN denotes As
T1865 6620-6625 VBN denotes shown
T1866 6692-6696 VBD denotes were
T1867 6626-6628 IN denotes in
T1868 6629-6635 NN denotes Figure
T1869 6636-6637 CD denotes 2
T1870 6637-6639 , denotes ,
T1871 6639-6643 NN denotes Cln3
T1872 6649-6657 NNS denotes isoforms
T1873 6644-6648 NN denotes mRNA
T1874 6658-6660 IN denotes in
T1875 6661-6665 JJ denotes wild
T1876 6666-6670 NN denotes type
T1877 6665-6666 HYPH denotes -
T1878 6686-6691 NNS denotes cells
T1879 6671-6674 CC denotes and
T1880 6675-6685 JJ denotes homozygous
T1881 6697-6704 JJ denotes similar
T1882 6705-6707 IN denotes to
T1883 6708-6713 DT denotes those
T1884 6714-6722 VBN denotes observed
T1885 6723-6725 IN denotes in
T1886 6726-6731 JJ denotes total
T1887 6732-6735 NN denotes RNA
T1888 6736-6744 VBN denotes isolated
T1889 6745-6749 IN denotes from
T1890 6750-6754 JJ denotes wild
T1891 6755-6759 NN denotes type
T1892 6754-6755 HYPH denotes -
T1893 6794-6799 NN denotes brain
T1894 6760-6762 CC denotes or
T1895 6763-6773 JJ denotes homozygous
T1896 6774-6782 NN denotes Cln3Δex7
T1897 6782-6783 HYPH denotes /
T1898 6783-6784 CD denotes 8
T1899 6785-6790 VB denotes knock
T1900 6790-6791 HYPH denotes -
T1901 6791-6793 RP denotes in
T1902 6799-6801 , denotes ,
T1903 6801-6813 RB denotes respectively
T1904 6814-6815 -LRB- denotes (
T1905 6815-6819 NN denotes Fig.
T1906 6820-6821 CD denotes 2
T1907 6821-6822 -RRB- denotes )
T1908 6822-6823 . denotes .
T1909 6823-7025 sentence denotes In addition, batp1 immunostaining detected mutant battenin product in homozygous CbCln3Δex7/8 cells, in a similar albeit reduced cytoplasmic, vesicular staining pattern as that seen in wild-type cells.
T1910 6824-6826 IN denotes In
T1911 6858-6866 VBD denotes detected
T1912 6827-6835 NN denotes addition
T1913 6835-6837 , denotes ,
T1914 6837-6842 NN denotes batp1
T1915 6843-6857 NN denotes immunostaining
T1916 6867-6873 NN denotes mutant
T1917 6883-6890 NN denotes product
T1918 6874-6882 NN denotes battenin
T1919 6891-6893 IN denotes in
T1920 6894-6904 JJ denotes homozygous
T1921 6918-6923 NNS denotes cells
T1922 6905-6915 NN denotes CbCln3Δex7
T1923 6915-6916 HYPH denotes /
T1924 6916-6917 CD denotes 8
T1925 6923-6925 , denotes ,
T1926 6925-6927 IN denotes in
T1927 6928-6929 DT denotes a
T1928 6985-6992 NN denotes pattern
T1929 6930-6937 JJ denotes similar
T1930 6938-6944 IN denotes albeit
T1931 6945-6952 VBN denotes reduced
T1932 6953-6964 JJ denotes cytoplasmic
T1933 6964-6966 , denotes ,
T1934 6966-6975 JJ denotes vesicular
T1935 6976-6984 NN denotes staining
T1936 6993-6995 IN denotes as
T1937 6996-7000 DT denotes that
T1938 7001-7005 VBN denotes seen
T1939 7006-7008 IN denotes in
T1940 7009-7013 JJ denotes wild
T1941 7014-7018 NN denotes type
T1942 7013-7014 HYPH denotes -
T1943 7019-7024 NNS denotes cells
T1944 7024-7025 . denotes .
T1945 7025-7211 sentence denotes Batp1 signal exhibited some overlap with the lysosomal marker, Lamp1, but had more significant overlap with early endosome antigen 1 (EEA1) and the late endosomal marker, Rab7 (Fig. 3).
T1946 7026-7031 NN denotes Batp1
T1947 7032-7038 NN denotes signal
T1948 7039-7048 VBD denotes exhibited
T1949 7049-7053 DT denotes some
T1950 7054-7061 NN denotes overlap
T1951 7062-7066 IN denotes with
T1952 7067-7070 DT denotes the
T1953 7081-7087 NN denotes marker
T1954 7071-7080 JJ denotes lysosomal
T1955 7087-7089 , denotes ,
T1956 7089-7094 NN denotes Lamp1
T1957 7094-7096 , denotes ,
T1958 7096-7099 CC denotes but
T1959 7100-7103 VBD denotes had
T1960 7104-7108 RBR denotes more
T1961 7109-7120 JJ denotes significant
T1962 7121-7128 NN denotes overlap
T1963 7129-7133 IN denotes with
T1964 7134-7139 JJ denotes early
T1965 7149-7156 NN denotes antigen
T1966 7140-7148 NN denotes endosome
T1967 7157-7158 CD denotes 1
T1968 7159-7160 -LRB- denotes (
T1969 7160-7164 NN denotes EEA1
T1970 7164-7165 -RRB- denotes )
T1971 7166-7169 CC denotes and
T1972 7170-7173 DT denotes the
T1973 7189-7195 NN denotes marker
T1974 7174-7178 JJ denotes late
T1975 7179-7188 JJ denotes endosomal
T1976 7195-7197 , denotes ,
T1977 7197-7201 NN denotes Rab7
T1978 7202-7203 -LRB- denotes (
T1979 7203-7207 NN denotes Fig.
T1980 7208-7209 CD denotes 3
T1981 7209-7210 -RRB- denotes )
T1982 7210-7211 . denotes .
T1983 7211-7339 sentence denotes Only limited overlap was observed with recycling endosomes, as determined by transferrin receptor co-staining (data not shown).
T1984 7212-7216 RB denotes Only
T1985 7225-7232 NN denotes overlap
T1986 7217-7224 VBN denotes limited
T1987 7237-7245 VBN denotes observed
T1988 7233-7236 VBD denotes was
T1989 7246-7250 IN denotes with
T1990 7251-7260 VBG denotes recycling
T1991 7261-7270 NNS denotes endosomes
T1992 7270-7272 , denotes ,
T1993 7272-7274 IN denotes as
T1994 7275-7285 VBN denotes determined
T1995 7286-7288 IN denotes by
T1996 7289-7300 NN denotes transferrin
T1997 7301-7309 NN denotes receptor
T1998 7310-7321 NN denotes co-staining
T1999 7322-7323 -LRB- denotes (
T2000 7332-7337 VBN denotes shown
T2001 7323-7327 NNS denotes data
T2002 7328-7331 RB denotes not
T2003 7337-7338 -RRB- denotes )
T2004 7338-7339 . denotes .
T2005 7339-7594 sentence denotes Intriguingly, Lamp1 and EEA1 immunocytochemical distribution were altered in homozygous CbCln3Δex7/8 cells, with less perinuclear clustering than in wild-type cells, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells (Fig. 3).
T2006 7340-7352 RB denotes Intriguingly
T2007 7406-7413 VBN denotes altered
T2008 7352-7354 , denotes ,
T2009 7354-7359 NN denotes Lamp1
T2010 7388-7400 NN denotes distribution
T2011 7360-7363 CC denotes and
T2012 7364-7368 NN denotes EEA1
T2013 7369-7387 JJ denotes immunocytochemical
T2014 7401-7405 VBD denotes were
T2015 7414-7416 IN denotes in
T2016 7417-7427 JJ denotes homozygous
T2017 7441-7446 NNS denotes cells
T2018 7428-7438 NN denotes CbCln3Δex7
T2019 7438-7439 HYPH denotes /
T2020 7439-7440 CD denotes 8
T2021 7446-7448 , denotes ,
T2022 7448-7452 IN denotes with
T2023 7453-7457 JJR denotes less
T2024 7470-7480 NN denotes clustering
T2025 7458-7469 JJ denotes perinuclear
T2026 7481-7485 IN denotes than
T2027 7486-7488 IN denotes in
T2028 7489-7493 JJ denotes wild
T2029 7494-7498 NN denotes type
T2030 7493-7494 HYPH denotes -
T2031 7499-7504 NNS denotes cells
T2032 7504-7506 , denotes ,
T2033 7506-7509 CC denotes and
T2034 7510-7514 NN denotes Rab7
T2035 7515-7523 NN denotes staining
T2036 7524-7527 VBD denotes was
T2037 7528-7538 RB denotes frequently
T2038 7539-7543 RBR denotes less
T2039 7544-7551 JJ denotes intense
T2040 7552-7554 IN denotes in
T2041 7555-7565 JJ denotes homozygous
T2042 7579-7584 NNS denotes cells
T2043 7566-7576 NN denotes CbCln3Δex7
T2044 7576-7577 HYPH denotes /
T2045 7577-7578 CD denotes 8
T2046 7585-7586 -LRB- denotes (
T2047 7586-7590 NN denotes Fig.
T2048 7591-7592 CD denotes 3
T2049 7592-7593 -RRB- denotes )
T2050 7593-7594 . denotes .
T2051 7594-7801 sentence denotes Heterozygous CbCln3Δex7/8 cells contained a mixture of Cln3 mRNA products from both the wild-type allele and the mutant allele, and batp1 signal was similar to that seen in wild-type cells (data not shown).
T2052 7595-7607 JJ denotes Heterozygous
T2053 7621-7626 NNS denotes cells
T2054 7608-7618 NN denotes CbCln3Δex7
T2055 7618-7619 HYPH denotes /
T2056 7619-7620 CD denotes 8
T2057 7627-7636 VBD denotes contained
T2058 7637-7638 DT denotes a
T2059 7639-7646 NN denotes mixture
T2060 7647-7649 IN denotes of
T2061 7650-7654 NN denotes Cln3
T2062 7660-7668 NNS denotes products
T2063 7655-7659 NN denotes mRNA
T2064 7669-7673 IN denotes from
T2065 7674-7678 CC denotes both
T2066 7693-7699 NN denotes allele
T2067 7679-7682 DT denotes the
T2068 7683-7687 JJ denotes wild
T2069 7688-7692 NN denotes type
T2128 9604-9608 WRB denotes when
T2129 9620-9624 VBN denotes aged
T2130 9609-9614 NNS denotes cells
T2131 9615-9619 VBD denotes were
T2132 9625-9627 IN denotes at
T2133 9628-9638 NN denotes confluency
T2134 9639-9640 -LRB- denotes (
T2135 9640-9641 CD denotes 3
T2136 9643-9647 NNS denotes days
T2137 9641-9642 SYM denotes +
T2138 9648-9663 RB denotes post-confluency
T2139 9663-9664 -RRB- denotes )
T2140 9664-9666 , denotes ,
T2141 9666-9676 JJ denotes homozygous
T2142 9709-9715 NNS denotes levels
T2143 9677-9687 NN denotes CbCln3Δex7
T2144 9687-9688 HYPH denotes /
T2145 9688-9689 CD denotes 8
T2146 9690-9698 JJ denotes cellular
T2147 9699-9706 NN denotes subunit
T2148 9707-9708 NN denotes c
T2149 9716-9720 VBD denotes were
T2150 9730-9736 IN denotes beyond
T2151 9737-9743 JJ denotes normal
T2152 9754-9760 NNS denotes levels
T2153 9744-9748 JJ denotes wild
T2154 9749-9753 NN denotes type
T2155 9748-9749 HYPH denotes -
T2156 9761-9763 IN denotes by
T2157 9764-9778 NN denotes immunostaining
T2158 9779-9780 -LRB- denotes (
T2159 9785-9787 NN denotes 4a
T2160 9780-9784 NN denotes Fig.
T2161 9787-9788 -RRB- denotes )
T2162 9789-9792 CC denotes and
T2163 9793-9803 NN denotes immunoblot
T2164 9804-9812 NN denotes analysis
T2165 9813-9814 -LRB- denotes (
T2166 9819-9821 NN denotes 4b
T2167 9814-9818 NN denotes Fig.
T2168 9821-9822 -RRB- denotes )
T2169 9822-9823 . denotes .
T2170 9823-9938 sentence denotes Autofluorescent signal sometimes overlapped with subunit c signal, but also was elevated more diffusely (Fig. 4a).
T2171 9824-9839 JJ denotes Autofluorescent
T2172 9840-9846 NN denotes signal
T2173 9857-9867 VBD denotes overlapped
T2174 9847-9856 RB denotes sometimes
T2175 9868-9872 IN denotes with
T2176 9873-9880 NN denotes subunit
T2177 9881-9882 NN denotes c
T2178 9883-9889 NN denotes signal
T2179 9889-9891 , denotes ,
T2180 9891-9894 CC denotes but
T2181 9895-9899 RB denotes also
T2182 9900-9903 VBD denotes was
T2183 9904-9912 JJ denotes elevated
T2184 9913-9917 RBR denotes more
T2185 9918-9927 RB denotes diffusely
T2186 9928-9929 -LRB- denotes (
T2187 9934-9936 NN denotes 4a
T2188 9929-9933 NN denotes Fig.
T2189 9936-9937 -RRB- denotes )
T2190 9937-9938 . denotes .
T2191 9938-10300 sentence denotes Moreover, although multilamellar "fingerprint" profiles were not detected, confluency-aged homozygous CbCln3Δex7/8 cells displayed numerous ultrastructural abnormalities including electron dense inclusions characteristic of lipofuscin and large autophagosomes that contained dense core structures, degenerating mitochondria, and many smaller vesicles (Fig. 4c).
T2192 9939-9947 RB denotes Moreover
T2193 10060-10069 VBD denotes displayed
T2194 9947-9949 , denotes ,
T2195 9949-9957 IN denotes although
T2196 10004-10012 VBN denotes detected
T2197 9958-9971 JJ denotes multilamellar
T2198 9986-9994 NNS denotes profiles
T2199 9972-9973 `` denotes "
T2200 9973-9984 NN denotes fingerprint
T2201 9984-9985 '' denotes "
T2202 9995-9999 VBD denotes were
T2203 10000-10003 RB denotes not
T2204 10012-10014 , denotes ,
T2205 10014-10024 JJ denotes confluency
T2206 10025-10029 VBN denotes aged
T2207 10024-10025 HYPH denotes -
T2208 10054-10059 NNS denotes cells
T2209 10030-10040 JJ denotes homozygous
T2210 10041-10051 NN denotes CbCln3Δex7
T2211 10051-10052 HYPH denotes /
T2212 10052-10053 CD denotes 8
T2213 10070-10078 JJ denotes numerous
T2214 10095-10108 NNS denotes abnormalities
T2215 10079-10094 JJ denotes ultrastructural
T2216 10109-10118 VBG denotes including
T2217 10119-10127 NN denotes electron
T2218 10128-10133 JJ denotes dense
T2219 10134-10144 NNS denotes inclusions
T2220 10145-10159 JJ denotes characteristic
T2221 10160-10162 IN denotes of
T2222 10163-10173 NN denotes lipofuscin
T2223 10174-10177 CC denotes and
T2224 10178-10183 JJ denotes large
T2225 10184-10198 NNS denotes autophagosomes
T2226 10199-10203 WDT denotes that
T2227 10204-10213 VBD denotes contained
T2228 10214-10219 JJ denotes dense
T2229 10225-10235 NNS denotes structures
T2230 10220-10224 NN denotes core
T2231 10235-10237 , denotes ,
T2232 10237-10249 VBG denotes degenerating
T2233 10250-10262 NNS denotes mitochondria
T2234 10262-10264 , denotes ,
T2235 10264-10267 CC denotes and
T2236 10268-10272 JJ denotes many
T2237 10281-10289 NNS denotes vesicles
T2238 10273-10280 JJR denotes smaller
T2239 10290-10291 -LRB- denotes (
T2240 10296-10298 NN denotes 4c
T2241 10291-10295 NNP denotes Fig.
T2242 10298-10299 -RRB- denotes )
T2243 10299-10300 . denotes .
T2244 10300-10419 sentence denotes Inclusion bodies and autophagosomes were infrequently observed in confluency-aged wild-type cultures (data not shown).
T2245 10301-10310 NN denotes Inclusion
T2246 10311-10317 NNS denotes bodies
T2247 10355-10363 VBN denotes observed
T2248 10318-10321 CC denotes and
T2249 10322-10336 NNS denotes autophagosomes
T2250 10337-10341 VBD denotes were
T2251 10342-10354 RB denotes infrequently
T2252 10364-10366 IN denotes in
T2253 10367-10377 NN denotes confluency
T2254 10378-10382 VBN denotes aged
T2255 10377-10378 HYPH denotes -
T2256 10393-10401 NNS denotes cultures
T2257 10383-10387 JJ denotes wild
T2258 10388-10392 NN denotes type
T2259 10387-10388 HYPH denotes -
T2260 10402-10403 -LRB- denotes (
T2261 10412-10417 VBN denotes shown
T2262 10403-10407 NNS denotes data
T2263 10408-10411 RB denotes not
T2264 10417-10418 -RRB- denotes )
T2265 10418-10419 . denotes .
T2526 11900-11910 JJ denotes Homozygous
T2527 11924-11929 NNS denotes cells
T2528 11911-11921 NN denotes CbCln3Δex7
T2529 11921-11922 HYPH denotes /
T2530 11922-11923 CD denotes 8
T2531 11959-11966 VBP denotes process
T2532 11930-11933 CC denotes and
T2533 11934-11942 NN denotes Cln3Δex7
T2534 11954-11958 NNS denotes mice
T2535 11942-11943 HYPH denotes /
T2536 11943-11944 CD denotes 8
T2537 11945-11950 VB denotes knock
T2538 11950-11951 HYPH denotes -
T2539 11951-11953 RP denotes in
T2540 11967-11976 NN denotes cathepsin
T2541 11977-11978 NN denotes D
T2542 11979-11989 RB denotes abnormally
T2543 11989-12182 sentence denotes We next investigated the basis for subunit c accumulation, testing the hypothesis that cathepsin D is abnormal since it is required for ATP synthase subunit c degradation in the lysosome [13].
T2544 11990-11992 PRP denotes We
T2545 11998-12010 VBD denotes investigated
T2546 11993-11997 RB denotes next
T2547 12011-12014 DT denotes the
T2548 12015-12020 NN denotes basis
T2549 12021-12024 IN denotes for
T2550 12025-12032 NN denotes subunit
T2551 12033-12034 NN denotes c
T2552 12035-12047 NN denotes accumulation
T2553 12047-12049 , denotes ,
T2554 12049-12056 VBG denotes testing
T2555 12057-12060 DT denotes the
T2556 12061-12071 NN denotes hypothesis
T2557 12072-12076 IN denotes that
T2558 12089-12091 VBZ denotes is
T2559 12077-12086 NN denotes cathepsin
T2560 12087-12088 NN denotes D
T2561 12092-12100 JJ denotes abnormal
T2562 12101-12106 IN denotes since
T2563 12110-12112 VBZ denotes is
T2564 12107-12109 PRP denotes it
T2565 12113-12121 JJ denotes required
T2566 12122-12125 IN denotes for
T2567 12126-12129 NN denotes ATP
T2568 12149-12160 NN denotes degradation
T2569 12130-12138 NN denotes synthase
T2570 12139-12146 NN denotes subunit
T2571 12147-12148 NN denotes c
T2572 12161-12163 IN denotes in
T2573 12164-12167 DT denotes the
T2574 12168-12176 NN denotes lysosome
T2575 12177-12178 -LRB- denotes [
T2576 12178-12180 CD denotes 13
T2577 12180-12181 -RRB- denotes ]
T2578 12181-12182 . denotes .
T2579 12182-12384 sentence denotes We first tested cathepsin D transport and processing in homozygous CbCln3Δex7/8 cells and Cln3Δex7/8 mice using anti-cathepsin D antibody that recognizes unprocessed and processed cathepsin D isoforms.
T2580 12183-12185 PRP denotes We
T2581 12192-12198 VBD denotes tested
T2582 12186-12191 RB denotes first
T2583 12199-12208 NN denotes cathepsin
T2584 12209-12210 NN denotes D
T2585 12211-12220 NN denotes transport
T2586 12221-12224 CC denotes and
T2587 12225-12235 NN denotes processing
T2588 12236-12238 IN denotes in
T2589 12239-12249 JJ denotes homozygous
T2590 12263-12268 NNS denotes cells
T2591 12250-12260 NN denotes CbCln3Δex7
T2592 12260-12261 HYPH denotes /
T2593 12261-12262 CD denotes 8
T2594 12269-12272 CC denotes and
T2595 12273-12281 NN denotes Cln3Δex7
T2596 12284-12288 NNS denotes mice
T2597 12281-12282 HYPH denotes /
T2598 12282-12283 CD denotes 8
T2599 12289-12294 VBG denotes using
T2600 12295-12309 JJ denotes anti-cathepsin
T2601 12310-12311 NN denotes D
T2602 12312-12320 NN denotes antibody
T2603 12321-12325 WDT denotes that
T2604 12326-12336 VBZ denotes recognizes
T2605 12337-12348 JJ denotes unprocessed
T2606 12375-12383 NNS denotes isoforms
T2607 12349-12352 CC denotes and
T2608 12353-12362 VBN denotes processed
T2609 12363-12372 NN denotes cathepsin
T2610 12373-12374 NN denotes D
T2611 12383-12384 . denotes .
T2612 12384-12633 sentence denotes Immunostaining of wild-type and homozygous CbCln3Δex7/8 cells revealed a perinuclear and punctate vesicular cathepsin D distribution, consistent with its transport and processing through the secretory pathway and delivery to the lysosome (Fig. 5a).
T2613 12385-12399 NN denotes Immunostaining
T2614 12447-12455 VBD denotes revealed
T2615 12400-12402 IN denotes of
T2616 12403-12407 JJ denotes wild
T2617 12408-12412 NN denotes type
T2618 12407-12408 HYPH denotes -
T2619 12441-12446 NNS denotes cells
T2620 12413-12416 CC denotes and
T2621 12417-12427 JJ denotes homozygous
T2622 12428-12438 NN denotes CbCln3Δex7
T2623 12438-12439 HYPH denotes /
T2624 12439-12440 CD denotes 8
T2625 12456-12457 DT denotes a
T2626 12505-12517 NN denotes distribution
T2627 12458-12469 JJ denotes perinuclear
T2628 12470-12473 CC denotes and
T2629 12474-12482 JJ denotes punctate
T2630 12483-12492 JJ denotes vesicular
T2631 12493-12502 NN denotes cathepsin
T2632 12503-12504 NN denotes D
T2633 12517-12519 , denotes ,
T2634 12519-12529 JJ denotes consistent
T2635 12530-12534 IN denotes with
T2636 12535-12538 PRP$ denotes its
T2637 12539-12548 NN denotes transport
T2638 12549-12552 CC denotes and
T2639 12553-12563 NN denotes processing
T2640 12564-12571 IN denotes through
T2641 12572-12575 DT denotes the
T2642 12586-12593 NN denotes pathway
T2643 12576-12585 JJ denotes secretory
T2644 12594-12597 CC denotes and
T2645 12598-12606 NN denotes delivery
T2646 12607-12609 IN denotes to
T2647 12610-12613 DT denotes the
T2648 12614-12622 NN denotes lysosome
T2649 12623-12624 -LRB- denotes (
T2650 12629-12631 NN denotes 5a
T2651 12624-12628 NN denotes Fig.
T2652 12631-12632 -RRB- denotes )
T2653 12632-12633 . denotes .
T2654 12633-12776 sentence denotes However, in homozygous CbCln3Δex7/8 cells, cathepsin D distribution was less vesicular and more perinuclear-clustered than in wild-type cells.
T2655 12634-12641 RB denotes However
T2656 12702-12705 VBD denotes was
T2657 12641-12643 , denotes ,
T2658 12643-12645 IN denotes in
T2659 12646-12656 JJ denotes homozygous
T2660 12670-12675 NNS denotes cells
T2661 12657-12667 NN denotes CbCln3Δex7
T2662 12667-12668 HYPH denotes /
T2663 12668-12669 CD denotes 8
T2664 12675-12677 , denotes ,
T2665 12677-12686 NN denotes cathepsin
T2666 12687-12688 NN denotes D
T2667 12689-12701 NN denotes distribution
T2668 12706-12710 RBR denotes less
T2669 12711-12720 JJ denotes vesicular
T2670 12721-12724 CC denotes and
T2671 12725-12729 RBR denotes more
T2672 12742-12751 VBN denotes clustered
T2673 12730-12741 JJ denotes perinuclear
T2674 12741-12742 HYPH denotes -
T2675 12752-12756 IN denotes than
T2676 12757-12759 IN denotes in
T2677 12760-12764 JJ denotes wild
T2678 12765-12769 NN denotes type
T2679 12764-12765 HYPH denotes -
T2680 12770-12775 NNS denotes cells
T2681 12775-12776 . denotes .
T2682 12776-12922 sentence denotes Immunoblots of homozygous CbCln3Δex7/8 cell and Cln3Δex7/8 tissue extracts also showed altered relative levels of cathepsin D isoforms (Fig. 5b).
T2683 12777-12788 NNS denotes Immunoblots
T2684 12857-12863 VBD denotes showed
T2685 12789-12791 IN denotes of
T2686 12792-12802 JJ denotes homozygous
T2687 12843-12851 NNS denotes extracts
T2688 12803-12813 NN denotes CbCln3Δex7
T2689 12816-12820 NN denotes cell
T2690 12813-12814 HYPH denotes /
T2691 12814-12815 CD denotes 8
T2692 12821-12824 CC denotes and
T2693 12825-12833 NN denotes Cln3Δex7
T2694 12836-12842 NN denotes tissue
T2695 12833-12834 HYPH denotes /
T2696 12834-12835 CD denotes 8
T2697 12852-12856 RB denotes also
T2698 12864-12871 VBN denotes altered
T2699 12881-12887 NNS denotes levels
T2700 12872-12880 JJ denotes relative
T2701 12888-12890 IN denotes of
T2702 12891-12900 NN denotes cathepsin
T2703 12901-12902 NN denotes D
T2704 12903-12911 NNS denotes isoforms
T2705 12912-12913 -LRB- denotes (
T2706 12918-12920 NN denotes 5b
T2707 12913-12917 NN denotes Fig.
T2708 12920-12921 -RRB- denotes )
T2709 12921-12922 . denotes .
T2710 12922-13156 sentence denotes Cathepsin D isoforms, identified by relative molecular weights, represent the ~45 kDa precursor, the ~43 kDa intermediate single chain form of the enzyme, and the 31 kDa heavy chain of the double-chain form of the mature enzyme [14].
T2711 12923-12932 NN denotes Cathepsin
T2712 12933-12934 NN denotes D
T2713 12935-12943 NNS denotes isoforms
T2714 12987-12996 VBP denotes represent
T2715 12943-12945 , denotes ,
T2716 12945-12955 VBN denotes identified
T2717 12956-12958 IN denotes by
T2718 12959-12967 JJ denotes relative
T2719 12978-12985 NNS denotes weights
T2720 12968-12977 JJ denotes molecular
T2721 12985-12987 , denotes ,
T2722 12997-13000 DT denotes the
T2723 13009-13018 NN denotes precursor
T2724 13001-13002 SYM denotes ~
T2725 13002-13004 CD denotes 45
T2726 13005-13008 NN denotes kDa
T2727 13018-13020 , denotes ,
T2728 13020-13023 DT denotes the
T2729 13058-13062 NN denotes form
T2730 13024-13025 SYM denotes ~
T2731 13025-13027 CD denotes 43
T2732 13028-13031 NN denotes kDa
T2733 13032-13044 JJ denotes intermediate
T2734 13045-13051 JJ denotes single
T2735 13052-13057 NN denotes chain
T2736 13063-13065 IN denotes of
T2737 13066-13069 DT denotes the
T2738 13070-13076 NN denotes enzyme
T2739 13076-13078 , denotes ,
T2740 13078-13081 CC denotes and
T2741 13082-13085 DT denotes the
T2742 13099-13104 NN denotes chain
T2743 13086-13088 CD denotes 31
T2744 13089-13092 NN denotes kDa
T2745 13093-13098 JJ denotes heavy
T2746 13105-13107 IN denotes of
T2747 13108-13111 DT denotes the
T2748 13125-13129 NN denotes form
T2749 13112-13118 JJ denotes double
T2750 13119-13124 NN denotes chain
T2751 13118-13119 HYPH denotes -
T2752 13130-13132 IN denotes of
T2753 13133-13136 DT denotes the
T2754 13144-13150 NN denotes enzyme
T2755 13137-13143 JJ denotes mature
T2756 13151-13152 -LRB- denotes [
T2757 13152-13154 CD denotes 14
T2758 13154-13155 -RRB- denotes ]
T2759 13155-13156 . denotes .
T2760 13156-13340 sentence denotes In homozygous CbCln3Δex7/8 cell and Cln3Δex7/8 tissue extracts, the precursor and heavy chains were reduced, and the single chain was slightly elevated compared to wild-type extracts.
T2761 13157-13159 IN denotes In
T2762 13257-13264 VBN denotes reduced
T2763 13160-13170 JJ denotes homozygous
T2764 13211-13219 NNS denotes extracts
T2765 13171-13181 NN denotes CbCln3Δex7
T2766 13184-13188 NN denotes cell
T2767 13181-13182 HYPH denotes /
T2768 13182-13183 CD denotes 8
T2769 13189-13192 CC denotes and
T2770 13193-13201 NN denotes Cln3Δex7
T2771 13204-13210 NN denotes tissue
T2772 13201-13202 HYPH denotes /
T2773 13202-13203 CD denotes 8
T2774 13219-13221 , denotes ,
T2775 13221-13224 DT denotes the
T2776 13225-13234 NN denotes precursor
T2777 13235-13238 CC denotes and
T2778 13239-13244 JJ denotes heavy
T2779 13245-13251 NNS denotes chains
T2780 13252-13256 VBD denotes were
T2781 13264-13266 , denotes ,
T2782 13266-13269 CC denotes and
T2783 13270-13273 DT denotes the
T2784 13281-13286 NN denotes chain
T2785 13274-13280 JJ denotes single
T2786 13287-13290 VBD denotes was
T2787 13291-13299 RB denotes slightly
T2788 13300-13308 JJ denotes elevated
T2789 13309-13317 VBN denotes compared
T2790 13318-13320 IN denotes to
T2838 13567-13568 DT denotes a
T2839 13587-13596 NN denotes phenotype
T2840 13569-13578 JJ denotes recessive
T2841 13579-13586 NN denotes disease
T2842 13597-13598 -LRB- denotes (
T2843 13607-13612 VBN denotes shown
T2844 13598-13602 NNS denotes data
T2845 13603-13606 RB denotes not
T2846 13612-13613 -RRB- denotes )
T2847 13613-13614 . denotes .
T2848 13614-13615 sentence denotes
T2889 14860-14869 JJ denotes enzymatic
T2890 14888-14891 IN denotes for
T2891 14892-14903 JJ denotes inefficient
T2892 14914-14922 NN denotes turnover
T2893 14904-14911 NN denotes subunit
T2894 14912-14913 NN denotes c
T2895 14922-14923 . denotes .
T2896 14923-15251 sentence denotes In a fluorogenic in vitro assay, cathepsin D activity in total cellular extracts was not significantly altered in homozygous CbCln3Δex7/8 cells (376 ± 89 RFU/μg total protein), versus wild-type cells (324 ± 58 RFU/μg total protein), although a consistent trend towards increased enzymatic activity in mutant cells was observed.
T2897 14924-14926 IN denotes In
T2898 15027-15034 VBN denotes altered
T2899 14927-14928 DT denotes a
T2900 14950-14955 NN denotes assay
T2901 14929-14940 JJ denotes fluorogenic
T2902 14941-14943 FW denotes in
T2903 14944-14949 FW denotes vitro
T2904 14955-14957 , denotes ,
T2905 14957-14966 NN denotes cathepsin
T2906 14967-14968 NN denotes D
T2907 14969-14977 NN denotes activity
T2908 14978-14980 IN denotes in
T2909 14981-14986 JJ denotes total
T2910 14996-15004 NNS denotes extracts
T2911 14987-14995 JJ denotes cellular
T2912 15005-15008 VBD denotes was
T2913 15009-15012 RB denotes not
T2914 15013-15026 RB denotes significantly
T2915 15035-15037 IN denotes in
T2916 15038-15048 JJ denotes homozygous
T2917 15062-15067 NNS denotes cells
T2918 15049-15059 NN denotes CbCln3Δex7
T2919 15059-15060 HYPH denotes /
T2920 15060-15061 CD denotes 8
T2921 15068-15069 -LRB- denotes (
T2922 15078-15081 NN denotes RFU
T2923 15069-15072 CD denotes 376
T2924 15075-15077 CD denotes 89
T2925 15073-15074 SYM denotes ±
T2926 15081-15082 SYM denotes /
T2927 15091-15098 NN denotes protein
T2928 15082-15084 NN denotes μg
T2929 15085-15090 JJ denotes total
T2930 15098-15099 -RRB- denotes )
T2931 15099-15101 , denotes ,
T2932 15101-15107 CC denotes versus
T2933 15108-15112 JJ denotes wild
T2934 15113-15117 NN denotes type
T2935 15112-15113 HYPH denotes -
T2936 15118-15123 NNS denotes cells
T2937 15124-15125 -LRB- denotes (
T2938 15134-15137 NN denotes RFU
T2939 15125-15128 CD denotes 324
T2940 15131-15133 CD denotes 58
T2941 15129-15130 IN denotes ±
T2942 15137-15138 SYM denotes /
T2943 15147-15154 NN denotes protein
T2944 15138-15140 NN denotes μg
T2945 15141-15146 JJ denotes total
T2946 15154-15155 -RRB- denotes )
T2947 15155-15157 , denotes ,
T2948 15157-15165 IN denotes although
T2949 15242-15250 VBN denotes observed
T2950 15166-15167 DT denotes a
T2951 15179-15184 NN denotes trend
T2952 15168-15178 JJ denotes consistent
T2953 15185-15192 IN denotes towards
T2954 15193-15202 VBN denotes increased
T2955 15213-15221 NN denotes activity
T2956 15203-15212 JJ denotes enzymatic
T2957 15222-15224 IN denotes in
T2958 15225-15231 NN denotes mutant
T2959 15232-15237 NNS denotes cells
T2960 15238-15241 VBD denotes was
T2961 15250-15251 . denotes .
T2962 15251-15419 sentence denotes Thus, cathepsin D transport and processing are disrupted in homozygous CbCln3Δex7/8 cells in a manner such that enzymatic activity appears to be relatively unaffected.
T2963 15252-15256 RB denotes Thus
T2964 15299-15308 VBN denotes disrupted
T2965 15256-15258 , denotes ,
T2966 15258-15267 NN denotes cathepsin
T2967 15268-15269 NN denotes D
T2968 15270-15279 NN denotes transport
T2969 15280-15283 CC denotes and
T2970 15284-15294 NN denotes processing
T2971 15295-15298 VBP denotes are
T2972 15309-15311 IN denotes in
T2973 15312-15322 JJ denotes homozygous
T2974 15336-15341 NNS denotes cells
T2975 15323-15333 NN denotes CbCln3Δex7
T2976 15333-15334 HYPH denotes /
T2977 15334-15335 CD denotes 8
T2978 15342-15344 IN denotes in
T2979 15345-15346 DT denotes a
T2980 15347-15353 NN denotes manner
T2981 15354-15358 JJ denotes such
T2982 15359-15363 IN denotes that
T2983 15383-15390 VBZ denotes appears
T2984 15364-15373 JJ denotes enzymatic
T2985 15374-15382 NN denotes activity
T2986 15391-15393 TO denotes to
T2987 15394-15396 VB denotes be
T2988 15397-15407 RB denotes relatively
T2989 15408-15418 JJ denotes unaffected
T2990 15418-15419 . denotes .
T3349 15421-15431 JJ denotes Homozygous
T3350 15445-15450 NNS denotes cells
T3351 15432-15442 NN denotes CbCln3Δex7
T3352 15442-15443 HYPH denotes /
T3353 15443-15444 CD denotes 8
T3354 15451-15455 VBP denotes show
T3355 15456-15464 JJ denotes abnormal
T3356 15474-15484 NNS denotes organelles
T3357 15465-15473 NN denotes membrane
T3358 15484-15705 sentence denotes The abnormal transport and processing of cathepsin D suggested membrane trafficking disruptions in homozygous CbCln3Δex7/8 cells; therefore, we surveyed the subcellular distribution and morphology of membrane organelles.
T3359 15485-15488 DT denotes The
T3360 15498-15507 NN denotes transport
T3361 15489-15497 JJ denotes abnormal
T3362 15538-15547 VBD denotes suggested
T3363 15508-15511 CC denotes and
T3364 15512-15522 NN denotes processing
T3365 15523-15525 IN denotes of
T3366 15526-15535 NN denotes cathepsin
T3367 15536-15537 NN denotes D
T3368 15629-15637 VBD denotes surveyed
T3369 15548-15556 NN denotes membrane
T3370 15557-15568 NN denotes trafficking
T3371 15569-15580 NNS denotes disruptions
T3372 15581-15583 IN denotes in
T3373 15584-15594 JJ denotes homozygous
T3374 15608-15613 NNS denotes cells
T3375 15595-15605 NN denotes CbCln3Δex7
T3376 15605-15606 HYPH denotes /
T3377 15606-15607 CD denotes 8
T3378 15613-15614 : denotes ;
T3379 15615-15624 RB denotes therefore
T3380 15624-15626 , denotes ,
T3381 15626-15628 PRP denotes we
T3382 15638-15641 DT denotes the
T3383 15654-15666 NN denotes distribution
T3384 15642-15653 JJ denotes subcellular
T3385 15667-15670 CC denotes and
T3386 15671-15681 NN denotes morphology
T3387 15682-15684 IN denotes of
T3388 15685-15693 NN denotes membrane
T3389 15694-15704 NNS denotes organelles
T3390 15704-15705 . denotes .
T3391 15705-15947 sentence denotes Components of the secretory pathway, including the ER, cis-Golgi, and trans-Golgi, did not appear altered from wild-type appearance when labeled with the respective markers, protein disulfide isomerase (PDI), GM130, and VVL (data not shown).
T3392 15706-15716 NNS denotes Components
T3393 15797-15803 VB denotes appear
T3394 15717-15719 IN denotes of
T3395 15720-15723 DT denotes the
T3396 15734-15741 NN denotes pathway
T3397 15724-15733 JJ denotes secretory
T3398 15741-15743 , denotes ,
T3465 16065-16066 CD denotes 8
T3399 15743-15752 VBG denotes including
T3400 15753-15756 DT denotes the
T3401 15757-15759 NN denotes ER
T3402 15759-15761 , denotes ,
T3403 15761-15764 NN denotes cis
T3404 15765-15770 NN denotes Golgi
T3405 15764-15765 HYPH denotes -
T3406 15770-15772 , denotes ,
T3407 15772-15775 CC denotes and
T3408 15776-15781 NN denotes trans
T3409 15782-15787 NN denotes Golgi
T3410 15781-15782 HYPH denotes -
T3411 15787-15789 , denotes ,
T3412 15789-15792 VBD denotes did
T3413 15793-15796 RB denotes not
T3414 15804-15811 JJ denotes altered
T3415 15812-15816 IN denotes from
T3416 15817-15821 JJ denotes wild
T3417 15822-15826 NN denotes type
T3418 15821-15822 HYPH denotes -
T3419 15827-15837 NN denotes appearance
T3420 15838-15842 WRB denotes when
T3421 15843-15850 VBN denotes labeled
T3422 15851-15855 IN denotes with
T3423 15856-15859 DT denotes the
T3424 15871-15878 NNS denotes markers
T3425 15860-15870 JJ denotes respective
T3426 15878-15880 , denotes ,
T3427 15880-15887 NN denotes protein
T3428 15898-15907 NN denotes isomerase
T3429 15888-15897 NN denotes disulfide
T3430 15908-15909 -LRB- denotes (
T3431 15909-15912 NN denotes PDI
T3432 15912-15913 -RRB- denotes )
T3433 15913-15915 , denotes ,
T3434 15915-15920 NN denotes GM130
T3435 15920-15922 , denotes ,
T3436 15922-15925 CC denotes and
T3437 15926-15929 NN denotes VVL
T3438 15930-15931 -LRB- denotes (
T3439 15940-15945 VBN denotes shown
T3440 15931-15935 NNS denotes data
T3441 15936-15939 RB denotes not
T3442 15945-15946 -RRB- denotes )
T3443 15946-15947 . denotes .
T3444 15947-16097 sentence denotes By contrast, the lysosomal markers, Lysotracker and Lamp 2 had significantly altered signal in homozygous CbCln3Δex7/8 cells, versus wild-type cells.
T3445 15948-15950 IN denotes By
T3446 16007-16010 VBD denotes had
T3447 15951-15959 NN denotes contrast
T3448 15959-15961 , denotes ,
T3449 15961-15964 DT denotes the
T3450 15975-15982 NNS denotes markers
T3451 15965-15974 JJ denotes lysosomal
T3452 15982-15984 , denotes ,
T3453 15984-15995 NN denotes Lysotracker
T3454 15996-15999 CC denotes and
T3455 16000-16004 NN denotes Lamp
T3456 16005-16006 CD denotes 2
T3457 16011-16024 RB denotes significantly
T3458 16025-16032 JJ denotes altered
T3459 16033-16039 NN denotes signal
T3460 16040-16042 IN denotes in
T3461 16043-16053 JJ denotes homozygous
T3462 16067-16072 NNS denotes cells
T3463 16054-16064 NN denotes CbCln3Δex7
T3464 16064-16065 HYPH denotes /
T3466 16072-16074 , denotes ,
T3467 16074-16080 CC denotes versus
T3468 16081-16085 JJ denotes wild
T3469 16086-16090 NN denotes type
T3470 16085-16086 HYPH denotes -
T3471 16091-16096 NNS denotes cells
T3472 16096-16097 . denotes .
T3473 16097-16361 sentence denotes Wild-type cells exhibited brightly stained lysosomes that were large and clustered in the perinuclear region whereas homozygous CbCln3Δex7/8 lysosomes were lightly stained, smaller vesicles that were more diffusely scattered in the cytoplasm of the cell (Fig. 6).
T3474 16098-16102 JJ denotes Wild
T3475 16103-16107 NN denotes type
T3476 16102-16103 HYPH denotes -
T3477 16108-16113 NNS denotes cells
T3478 16114-16123 VBD denotes exhibited
T3479 16124-16132 RB denotes brightly
T3480 16133-16140 VBN denotes stained
T3481 16141-16150 NNS denotes lysosomes
T3482 16151-16155 WDT denotes that
T3483 16156-16160 VBD denotes were
T3484 16161-16166 JJ denotes large
T3485 16167-16170 CC denotes and
T3486 16171-16180 JJ denotes clustered
T3487 16181-16183 IN denotes in
T3488 16184-16187 DT denotes the
T3489 16200-16206 NN denotes region
T3490 16188-16199 JJ denotes perinuclear
T3491 16207-16214 IN denotes whereas
T3492 16249-16253 VBD denotes were
T3493 16215-16225 JJ denotes homozygous
T3494 16239-16248 NNS denotes lysosomes
T3495 16226-16236 NN denotes CbCln3Δex7
T3496 16236-16237 HYPH denotes /
T3497 16237-16238 CD denotes 8
T3498 16254-16261 RB denotes lightly
T3499 16262-16269 VBN denotes stained
T3500 16279-16287 NNS denotes vesicles
T3501 16269-16271 , denotes ,
T3502 16271-16278 JJR denotes smaller
T3503 16288-16292 WDT denotes that
T3504 16313-16322 VBN denotes scattered
T3505 16293-16297 VBD denotes were
T3506 16298-16302 RBR denotes more
T3507 16303-16312 RB denotes diffusely
T3508 16323-16325 IN denotes in
T3509 16326-16329 DT denotes the
T3510 16330-16339 NN denotes cytoplasm
T3511 16340-16342 IN denotes of
T3512 16343-16346 DT denotes the
T3513 16347-16351 NN denotes cell
T3514 16352-16353 -LRB- denotes (
T3515 16353-16357 NN denotes Fig.
T3516 16358-16359 CD denotes 6
T3517 16359-16360 -RRB- denotes )
T3518 16360-16361 . denotes .
T3519 16361-16429 sentence denotes Lamp 1 distribution was also altered, as previously noted (Fig. 3).
T3520 16362-16366 NN denotes Lamp
T3521 16369-16381 NN denotes distribution
T3522 16367-16368 CD denotes 1
T3523 16391-16398 VBN denotes altered
T3524 16382-16385 VBD denotes was
T3525 16386-16390 RB denotes also
T3526 16398-16400 , denotes ,
T3527 16400-16402 IN denotes as
T3528 16414-16419 VBN denotes noted
T3533 16427-16428 -RRB- denotes )
T3534 16428-16429 . denotes .
T3535 16429-16697 sentence denotes However, Lamp 1 and Lamp 2 total protein levels were similar in wild-type and homozygous CbCln3Δex7/8 cells by immunoblot analysis, indicating the altered signal likely reflects dispersed lysosomes or altered localization and/or epitope availability (data not shown).
T3536 16430-16437 RB denotes However
T3537 16478-16482 VBD denotes were
T3538 16437-16439 , denotes ,
T3539 16439-16443 NN denotes Lamp
T3540 16471-16477 NNS denotes levels
T3541 16444-16445 CD denotes 1
T3542 16446-16449 CC denotes and
T3543 16450-16454 NN denotes Lamp
T3544 16455-16456 CD denotes 2
T3545 16457-16462 JJ denotes total
T3546 16463-16470 NN denotes protein
T3547 16483-16490 JJ denotes similar
T3548 16491-16493 IN denotes in
T3549 16494-16498 JJ denotes wild
T3550 16499-16503 NN denotes type
T3551 16498-16499 HYPH denotes -
T3552 16532-16537 NNS denotes cells
T3553 16504-16507 CC denotes and
T3554 16508-16518 JJ denotes homozygous
T3555 16519-16529 NN denotes CbCln3Δex7
T3556 16529-16530 HYPH denotes /
T3557 16530-16531 CD denotes 8
T3558 16538-16540 IN denotes by
T3559 16541-16551 NN denotes immunoblot
T3560 16552-16560 NN denotes analysis
T3561 16560-16562 , denotes ,
T3562 16562-16572 VBG denotes indicating
T3563 16573-16576 DT denotes the
T3564 16585-16591 NN denotes signal
T3565 16577-16584 JJ denotes altered
T3566 16599-16607 VBZ denotes reflects
T3567 16592-16598 RB denotes likely
T3568 16608-16617 JJ denotes dispersed
T3569 16618-16627 NNS denotes lysosomes
T3570 16628-16630 CC denotes or
T3571 16631-16638 JJ denotes altered
T3572 16639-16651 NN denotes localization
T3573 16652-16655 CC denotes and
T3574 16655-16656 HYPH denotes /
T3575 16656-16658 CC denotes or
T3576 16659-16666 NN denotes epitope
T3577 16667-16679 NN denotes availability
T3578 16680-16681 -LRB- denotes (
T3579 16690-16695 VBN denotes shown
T3580 16681-16685 NNS denotes data
T3581 16686-16689 RB denotes not
T3582 16695-16696 -RRB- denotes )
T3583 16696-16697 . denotes .
T3584 16697-16849 sentence denotes It is noteworthy that Lysotracker dye, which selectively accumulates in acidic compartments, exhibited the most marked reduction in lysosomal labeling.
T3585 16698-16700 PRP denotes It
T3586 16701-16703 VBZ denotes is
T3587 16704-16714 JJ denotes noteworthy
T3588 16715-16719 IN denotes that
T3589 16791-16800 VBD denotes exhibited
T3590 16720-16731 NN denotes Lysotracker
T3591 16732-16735 NN denotes dye
T3592 16735-16737 , denotes ,
T3593 16737-16742 WDT denotes which
T3594 16755-16766 VBZ denotes accumulates
T3595 16743-16754 RB denotes selectively
T3596 16767-16769 IN denotes in
T3597 16770-16776 JJ denotes acidic
T3598 16777-16789 NNS denotes compartments
T3599 16789-16791 , denotes ,
T3600 16801-16804 DT denotes the
T3601 16817-16826 NN denotes reduction
T3602 16805-16809 RBS denotes most
T3603 16810-16816 JJ denotes marked
T3604 16827-16829 IN denotes in
T3605 16830-16839 JJ denotes lysosomal
T3606 16840-16848 NN denotes labeling
T3607 16848-16849 . denotes .
T3608 16849-16940 sentence denotes This observation may reflect altered lysosomal pH, an established finding in JNCL [10,15].
T3609 16850-16854 DT denotes This
T3610 16855-16866 NN denotes observation
T3611 16871-16878 VB denotes reflect
T3612 16867-16870 MD denotes may
T3613 16879-16886 JJ denotes altered
T3614 16897-16899 NN denotes pH
T3615 16887-16896 JJ denotes lysosomal
T3616 16899-16901 , denotes ,
T3617 16901-16903 DT denotes an
T3618 16916-16923 NN denotes finding
T3619 16904-16915 JJ denotes established
T3620 16924-16926 IN denotes in
T3621 16927-16931 NN denotes JNCL
T3622 16932-16933 -LRB- denotes [
T3623 16936-16938 CD denotes 15
T3624 16933-16935 CD denotes 10
T3625 16935-16936 , denotes ,
T3626 16938-16939 -RRB- denotes ]
T3627 16939-16940 . denotes .
T3628 16940-16941 sentence denotes
T3629 17823-17835 sentence denotes Consistent w
T3695 17999-18001 IN denotes as
T3696 18002-18010 VBN denotes measured
T3697 18011-18013 IN denotes by
T3698 18014-18021 NN denotes dextran
T3699 18022-18026 NN denotes FITC
T3700 18021-18022 HYPH denotes -
T3701 18027-18033 NN denotes uptake
T3702 18034-18035 -LRB- denotes (
T3703 18035-18039 NN denotes Fig.
T3704 18040-18041 CD denotes 7
T3705 18041-18042 -RRB- denotes )
T3706 18042-18043 . denotes .
T3707 18043-18243 sentence denotes Following a 15-minute incubation in media containing dextran-FITC, wild-type and heterozygote cells displayed brightly stained, large endocytic vesicles that were clustered in the perinuclear region.
T3708 18044-18053 VBG denotes Following
T3709 18144-18153 VBD denotes displayed
T3710 18054-18055 DT denotes a
T3711 18066-18076 NN denotes incubation
T3712 18056-18058 CD denotes 15
T3713 18059-18065 NN denotes minute
T3749 18274-18275 HYPH denotes /
T3750 18275-18276 CD denotes 8
T3751 18283-18287 VBD denotes were
T3752 18288-18292 RBR denotes less
T3753 18293-18301 RB denotes brightly
T3754 18310-18314 IN denotes with
T3755 18315-18319 JJS denotes most
T3756 18333-18339 NN denotes signal
T3757 18320-18327 NN denotes dextran
T3758 18328-18332 NN denotes FITC
T3759 18327-18328 HYPH denotes -
T3760 18340-18350 VBG denotes localizing
T3761 18351-18353 IN denotes to
T3762 18354-18361 JJR denotes smaller
T3763 18362-18370 NNS denotes vesicles
T3764 18371-18380 VBN denotes scattered
T3765 18381-18391 IN denotes throughout
T3766 18392-18395 DT denotes the
T3767 18396-18405 NN denotes cytoplasm
T3768 18406-18408 IN denotes of
T3769 18409-18412 DT denotes the
T3770 18413-18417 NN denotes cell
T3771 18417-18418 . denotes .
T3772 18418-18421 sentence denotes Fi
T3773 18419-18421 NN denotes Fi
T3774 19108-19118 sentence denotes nally, bec
T3775 19108-19109 NN denotes n
T3776 19111-19112 NNS denotes l
T3777 19109-19110 CC denotes a
T3778 19110-19111 NN denotes l
T3779 19112-19113 NN denotes y
T3780 19113-19115 , denotes ,
T3781 19115-19117 NN denotes be
T3782 19117-19118 NN denotes c
T3783 19118-19126 sentence denotes ause sub
T3784 19123-19124 NNS denotes s
T3809 19160-19163 CC denotes and
T3810 19164-19167 PRP$ denotes its
T3811 19168-19176 NN denotes turnover
T3812 19177-19185 VBZ denotes proceeds
T3813 19186-19193 IN denotes through
T3814 19194-19204 JJ denotes autophagic
T3815 19205-19215 NN denotes engulfment
T3816 19216-19218 IN denotes of
T3817 19219-19231 NNS denotes mitochondria
T3818 19232-19233 -LRB- denotes [
T3819 19233-19235 CD denotes 13
T3820 19235-19236 -RRB- denotes ]
T3821 19236-19238 , denotes ,
T3822 19238-19240 PRP denotes we
T3823 19250-19260 JJ denotes homozygous
T3824 19261-19271 NN denotes CbCln3Δex7
T3825 19271-19272 HYPH denotes /
T3826 19272-19273 CD denotes 8
T3827 19274-19278 NN denotes cell
T3828 19279-19292 JJ denotes mitochondrial
T3829 19293-19303 NN denotes morphology
T3830 19304-19307 CC denotes and
T3831 19308-19316 NN denotes function
T3832 19316-19317 . denotes .
T3833 19317-19567 sentence denotes Mitochondrial distribution in homozygous CbCln3Δex7/8 cells was indistinguishable from wild-type and heterozygous cells; however, homozygous CbCln3Δex7/8 mitochondria appeared more elongated by grp75 marker immunostaining and TEM analysis (Fig. 8a).
T3834 19318-19331 JJ denotes Mitochondrial
T3835 19332-19344 NN denotes distribution
T3836 19378-19381 VBD denotes was
T3837 19345-19347 IN denotes in
T3838 19348-19358 JJ denotes homozygous
T3839 19372-19377 NNS denotes cells
T3840 19359-19369 NN denotes CbCln3Δex7
T3841 19369-19370 HYPH denotes /
T3842 19370-19371 CD denotes 8
T3843 19485-19493 VBD denotes appeared
T3844 19382-19399 JJ denotes indistinguishable
T3845 19400-19404 IN denotes from
T3846 19405-19409 JJ denotes wild
T3847 19410-19414 NN denotes type
T3848 19409-19410 HYPH denotes -
T3849 19432-19437 NNS denotes cells
T3850 19415-19418 CC denotes and
T3851 19419-19431 JJ denotes heterozygous
T3852 19437-19438 : denotes ;
T3853 19439-19446 RB denotes however
T3854 19446-19448 , denotes ,
T3855 19448-19458 JJ denotes homozygous
T3856 19472-19484 NNS denotes mitochondria
T3857 19459-19469 NN denotes CbCln3Δex7
T3858 19469-19470 HYPH denotes /
T3859 19470-19471 CD denotes 8
T3860 19494-19498 RBR denotes more
T3861 19499-19508 JJ denotes elongated
T3862 19509-19511 IN denotes by
T3863 19512-19517 NN denotes grp75
T3864 19525-19539 NN denotes immunostaining
T3865 19518-19524 NN denotes marker
T3866 19540-19543 CC denotes and
T3867 19544-19547 NN denotes TEM
T3868 19548-19556 NN denotes analysis
T3869 19557-19558 -LRB- denotes (
T3870 19563-19565 NN denotes 8a
T3871 19558-19562 NN denotes Fig.
T3872 19565-19566 -RRB- denotes )
T3873 19566-19567 . denotes .
T3874 19567-19761 sentence denotes 72% of homozygous mutant mitochondria were greater than 0.6 μm in length (range = 0.26 μm to 2.75 μm), while fewer wild-type mitochondria (51%) reached this length (range = 0.15 μm to 2.29 μm).
T3875 19568-19570 CD denotes 72
T3876 19570-19571 NN denotes %
T3877 19606-19610 VBD denotes were
T3878 19572-19574 IN denotes of
T3879 19575-19585 JJ denotes homozygous
T3880 19593-19605 NNS denotes mitochondria
T3881 19586-19592 NN denotes mutant
T3882 19611-19618 JJR denotes greater
T3883 19624-19627 CD denotes 0.6
T3884 19619-19623 IN denotes than
T3885 19628-19630 NN denotes μm
T3886 19631-19633 IN denotes in
T3887 19634-19640 NN denotes length
T3888 19641-19642 -LRB- denotes (
T3889 19655-19657 NN denotes μm
T3890 19642-19647 NN denotes range
T3891 19648-19649 SYM denotes =
T3892 19650-19654 CD denotes 0.26
T3893 19658-19660 IN denotes to
T3894 19661-19665 CD denotes 2.75
T3895 19666-19668 NN denotes μm
T3896 19668-19669 -RRB- denotes )
T3897 19669-19671 , denotes ,
T3898 19671-19676 IN denotes while
T3899 19712-19719 VBD denotes reached
T3900 19677-19682 JJR denotes fewer
T3901 19693-19705 NNS denotes mitochondria
T3902 19683-19687 JJ denotes wild
T3903 19688-19692 NN denotes type
T3904 19687-19688 HYPH denotes -
T3905 19706-19707 -LRB- denotes (
T3906 19709-19710 NN denotes %
T3907 19707-19709 CD denotes 51
T3908 19710-19711 -RRB- denotes )
T3909 19720-19724 DT denotes this
T3910 19725-19731 NN denotes length
T3911 19732-19733 -LRB- denotes (
T3912 19746-19748 NN denotes μm
T3913 19733-19738 NN denotes range
T3914 19739-19740 SYM denotes =
T3915 19741-19745 CD denotes 0.15
T3916 19749-19751 IN denotes to
T3917 19752-19756 CD denotes 2.29
T3918 19757-19759 NN denotes μm
T3919 19759-19760 -RRB- denotes )
T3920 19760-19761 . denotes .
T3921 19761-19848 sentence denotes Mitochondrial width was not altered in homozygous CbCln3Δex7/8 cells (data not shown).
T3922 19762-19775 JJ denotes Mitochondrial
T3923 19776-19781 NN denotes width
T3924 19790-19797 VBN denotes altered
T3925 19782-19785 VBD denotes was
T3926 19786-19789 RB denotes not
T3927 19798-19800 IN denotes in
T3928 19801-19811 JJ denotes homozygous
T3929 19825-19830 NNS denotes cells
T3930 19812-19822 NN denotes CbCln3Δex7
T3931 19822-19823 HYPH denotes /
T3932 19823-19824 CD denotes 8
T3933 19831-19832 -LRB- denotes (
T3934 19841-19846 VBN denotes shown
T3935 19832-19836 NNS denotes data
T3936 19837-19840 RB denotes not
T3937 19846-19847 -RRB- denotes )
T3938 19847-19848 . denotes .
T3939 19848-20181 sentence denotes Moreover, compared to wild-type or heterozygous cells, homozygous CbCln3Δex7/8 cells had significantly reduced cellular ATP levels (1.3 fold less, Fig. 8b) and exhibited reduced survival following hydrogen peroxide treatment (~50% of wild-type survival, Fig. 8c), suggesting impaired energy metabolism and oxidative stress response.
T3940 19849-19857 RB denotes Moreover
T3941 19934-19937 VBD denotes had
T3942 19857-19859 , denotes ,
T3943 19859-19867 VBN denotes compared
T3944 19868-19870 IN denotes to
T3945 19871-19875 JJ denotes wild
T3946 19876-19880 NN denotes type
T3947 19875-19876 HYPH denotes -
T3948 19897-19902 NNS denotes cells
T3949 19881-19883 CC denotes or
T3950 19884-19896 JJ denotes heterozygous
T3951 19902-19904 , denotes ,
T3952 19904-19914 JJ denotes homozygous
T3953 19928-19933 NNS denotes cells
T3954 19915-19925 NN denotes CbCln3Δex7
T3955 19925-19926 HYPH denotes /
T3956 19926-19927 CD denotes 8
T3957 19938-19951 RB denotes significantly
T3958 19952-19959 VBN denotes reduced
T3959 19973-19979 NNS denotes levels
T3960 19960-19968 JJ denotes cellular
T3961 19969-19972 NN denotes ATP
T3962 19980-19981 -LRB- denotes (
T3963 19996-20000 NN denotes Fig.
T3964 19981-19984 CD denotes 1.3
T3965 19990-19994 JJR denotes less
T3966 19985-19989 RB denotes fold
T3967 19994-19996 , denotes ,
T3968 20001-20003 CD denotes 8b
T3969 20003-20004 -RRB- denotes )
T3970 20005-20008 CC denotes and
T3971 20009-20018 VBD denotes exhibited
T3972 20019-20026 VBN denotes reduced
T3973 20027-20035 NN denotes survival
T3974 20036-20045 VBG denotes following
T3975 20046-20054 NN denotes hydrogen
T3976 20055-20063 NN denotes peroxide
T3977 20064-20073 NN denotes treatment
T3978 20074-20075 -LRB- denotes (
T3979 20108-20110 NN denotes 8c
T3980 20075-20076 SYM denotes ~
T3981 20076-20078 CD denotes 50
T3982 20078-20079 NN denotes %
T3983 20080-20082 IN denotes of
T3984 20083-20087 JJ denotes wild
T3985 20088-20092 NN denotes type
T3986 20087-20088 HYPH denotes -
T3987 20093-20101 NN denotes survival
T3988 20101-20103 , denotes ,
T3989 20103-20107 NN denotes Fig.
T3990 20110-20111 -RRB- denotes )
T3991 20111-20113 , denotes ,
T3992 20113-20123 VBG denotes suggesting
T3993 20124-20132 JJ denotes impaired
T3994 20140-20150 NN denotes metabolism
T3995 20133-20139 NN denotes energy
T3996 20151-20154 CC denotes and
T3997 20155-20164 JJ denotes oxidative
T3998 20172-20180 NN denotes response
T3999 20165-20171 NN denotes stress
T4000 20180-20181 . denotes .
T4001 20181-20282 sentence denotes Taken together, these data support impaired mitochondrial function in homozygous CbCln3Δex7/8 cells.
T4002 20182-20187 VBN denotes Taken
T4003 20209-20216 VBP denotes support
T4004 20188-20196 RB denotes together
T4005 20196-20198 , denotes ,
T4006 20198-20203 DT denotes these
T4007 20204-20208 NNS denotes data
T4008 20217-20225 JJ denotes impaired
T4009 20240-20248 NN denotes function
T4010 20226-20239 JJ denotes mitochondrial
T4011 20249-20251 IN denotes in
T4012 20252-20262 JJ denotes homozygous
T4013 20276-20281 NNS denotes cells
T4014 20263-20273 NN denotes CbCln3Δex7
T4015 20273-20274 HYPH denotes /
T4016 20274-20275 CD denotes 8
T4017 20281-20282 . denotes .
T4415 21950-21960 NN denotes CbCln3Δex7
T4416 21984-21989 NNS denotes cells
T4417 21960-21961 HYPH denotes /
T4418 21961-21962 CD denotes 8
T4419 21963-21973 JJ denotes cerebellar
T4420 21974-21983 NN denotes precursor
T4421 21990-21999 VBP denotes represent
T4422 22000-22003 DT denotes the
T4423 22054-22059 NN denotes model
T4424 22004-22009 JJ denotes first
T4425 22010-22021 RB denotes genetically
T4426 22022-22030 JJ denotes accurate
T4427 22031-22037 NN denotes neuron
T4428 22038-22045 JJ denotes derived
T4429 22037-22038 HYPH denotes -
T4430 22046-22053 NN denotes culture
T4431 22060-22062 IN denotes of
T4432 22063-22067 NN denotes JNCL
T4433 22067-22068 . denotes .
T4434 22068-22220 sentence denotes Homozygous CbCln3Δex7/8 cells express mutant battenin and JNCL-hallmark mitochondrial ATPase subunit c accumulation, upon aging of cells at confluency.
T4435 22069-22079 JJ denotes Homozygous
T4436 22093-22098 NNS denotes cells
T4437 22080-22090 NN denotes CbCln3Δex7
T4438 22090-22091 HYPH denotes /
T4439 22091-22092 CD denotes 8
T4440 22099-22106 VBP denotes express
T4441 22107-22113 NN denotes mutant
T4442 22114-22122 NN denotes battenin
T4443 22123-22126 CC denotes and
T4444 22127-22131 NN denotes JNCL
T4445 22132-22140 NN denotes hallmark
T4446 22131-22132 HYPH denotes -
T4447 22172-22184 NN denotes accumulation
T4448 22141-22154 JJ denotes mitochondrial
T4449 22155-22161 NN denotes ATPase
T4450 22162-22169 NN denotes subunit
T4451 22170-22171 NN denotes c
T4452 22184-22186 , denotes ,
T4453 22186-22190 IN denotes upon
T4454 22191-22196 NN denotes aging
T4455 22197-22199 IN denotes of
T4456 22200-22205 NNS denotes cells
T4457 22206-22208 IN denotes at
T4458 22209-22219 NN denotes confluency
T4459 22219-22220 . denotes .
T4460 22220-22417 sentence denotes Moreover, this is the first study to indicate recessive endosomal/lysosomal membrane trafficking defects and mitochondrial dysfunction that precedes subunit c deposition in an accurate JNCL model.
T4461 22221-22229 RB denotes Moreover
T4462 22236-22238 VBZ denotes is
T4463 22229-22231 , denotes ,
T4464 22231-22235 DT denotes this
T4465 22239-22242 DT denotes the
T4466 22249-22254 NN denotes study
T4467 22243-22248 JJ denotes first
T4468 22255-22257 TO denotes to
T4469 22258-22266 VB denotes indicate
T4470 22267-22276 JJ denotes recessive
T4471 22318-22325 NNS denotes defects
T4472 22277-22286 JJ denotes endosomal
T4473 22287-22296 JJ denotes lysosomal
T4474 22286-22287 HYPH denotes /
T4475 22297-22305 NN denotes membrane
T4476 22306-22317 NN denotes trafficking
T4477 22326-22329 CC denotes and
T4478 22330-22343 JJ denotes mitochondrial
T4479 22344-22355 NN denotes dysfunction
T4480 22356-22360 WDT denotes that
T4481 22361-22369 VBZ denotes precedes
T4482 22370-22377 NN denotes subunit
T4483 22378-22379 NN denotes c
T4484 22380-22390 NN denotes deposition
T4485 22391-22393 IN denotes in
T4486 22394-22396 DT denotes an
T4487 22411-22416 NN denotes model
T4488 22397-22405 JJ denotes accurate
T4489 22406-22410 NN denotes JNCL
T4490 22416-22417 . denotes .
T4491 22417-22549 sentence denotes Importantly, these defects are likely to be early events in the JNCL disease process and may particularly impact neuronal function.
T4492 22418-22429 RB denotes Importantly
T4493 22445-22448 VBP denotes are
T4494 22429-22431 , denotes ,
T4495 22431-22436 DT denotes these
T4496 22437-22444 NNS denotes defects
T4497 22449-22455 JJ denotes likely
T4498 22456-22458 TO denotes to
T4499 22459-22461 VB denotes be
T4500 22462-22467 JJ denotes early
T4501 22468-22474 NNS denotes events
T4502 22475-22477 IN denotes in
T4503 22478-22481 DT denotes the
T4504 22495-22502 NN denotes process
T4505 22482-22486 NN denotes JNCL
T4506 22487-22494 NN denotes disease
T4507 22503-22506 CC denotes and
T4508 22507-22510 MD denotes may
T4509 22524-22530 VB denotes impact
T4510 22511-22523 RB denotes particularly
T4511 22531-22539 JJ denotes neuronal
T4512 22540-22548 NN denotes function
T4513 22548-22549 . denotes .
T4514 22549-22775 sentence denotes Abnormal cathepsin D localization and processing in homozygous CbCln3Δex7/8 cells and Cln3Δex7/8 mice likely reflects altered vesicular trafficking and/or lysosomal pH, which is known to impact cathepsin D processing [14,16].
T4515 22550-22558 JJ denotes Abnormal
T4516 22569-22570 NN denotes D
T4517 22559-22568 NN denotes cathepsin
T4518 22659-22667 VBZ denotes reflects
T4519 22571-22583 NN denotes localization
T4520 22584-22587 CC denotes and
T4521 22588-22598 NN denotes processing
T4522 22599-22601 IN denotes in
T4523 22602-22612 JJ denotes homozygous
T4524 22626-22631 NNS denotes cells
T4525 22613-22623 NN denotes CbCln3Δex7
T4526 22623-22624 HYPH denotes /
T4527 22624-22625 CD denotes 8
T4528 22632-22635 CC denotes and
T4529 22636-22644 NN denotes Cln3Δex7
T4530 22647-22651 NNS denotes mice
T4531 22644-22645 HYPH denotes /
T4532 22645-22646 CD denotes 8
T4533 22652-22658 RB denotes likely
T4534 22668-22675 VBN denotes altered
T4535 22686-22697 NN denotes trafficking
T4536 22676-22685 JJ denotes vesicular
T4537 22698-22701 CC denotes and
T4538 22701-22702 HYPH denotes /
T4539 22702-22704 CC denotes or
T4540 22705-22714 JJ denotes lysosomal
T4541 22715-22717 NN denotes pH
T4542 22717-22719 , denotes ,
T4543 22719-22724 WDT denotes which
T4544 22728-22733 VBN denotes known
T4545 22725-22727 VBZ denotes is
T4546 22734-22736 TO denotes to
T4547 22737-22743 VB denotes impact
T4548 22744-22753 NN denotes cathepsin
T4549 22754-22755 NN denotes D
T4550 22756-22766 NN denotes processing
T4551 22767-22768 -LRB- denotes [
T4552 22771-22773 CD denotes 16
T4553 22768-22770 CD denotes 14
T4554 22770-22771 , denotes ,
T4555 22773-22774 -RRB- denotes ]
T4556 22774-22775 . denotes .
T4557 22775-22933 sentence denotes Indeed, CLN3 overexpression in HEK-293 cells altered lysosomal pH and cathepsin D processing [17], and lysosomal pH homeostasis is disrupted in JNCL [10,15].
T4558 22776-22782 RB denotes Indeed
T4559 22821-22828 VBD denotes altered
T4620 23037-23049 RB denotes Nevertheless
T4560 22782-22784 , denotes ,
T4561 22784-22788 NN denotes CLN3
T4562 22789-22803 NN denotes overexpression
T4563 22804-22806 IN denotes in
T4564 22807-22810 NN denotes HEK
T4565 22815-22820 NNS denotes cells
T4566 22810-22811 HYPH denotes -
T4567 22811-22814 CD denotes 293
T4568 22829-22838 JJ denotes lysosomal
T4569 22839-22841 NN denotes pH
T4570 22858-22868 NN denotes processing
T4571 22842-22845 CC denotes and
T4572 22846-22855 NN denotes cathepsin
T4573 22856-22857 NN denotes D
T4574 22869-22870 -LRB- denotes [
T4575 22870-22872 CD denotes 17
T4576 22872-22873 -RRB- denotes ]
T4577 22873-22875 , denotes ,
T4578 22875-22878 CC denotes and
T4579 22879-22888 JJ denotes lysosomal
T4580 22889-22891 NN denotes pH
T4581 22892-22903 NN denotes homeostasis
T4582 22907-22916 VBN denotes disrupted
T4583 22904-22906 VBZ denotes is
T4584 22917-22919 IN denotes in
T4585 22920-22924 NN denotes JNCL
T4586 22925-22926 -LRB- denotes [
T4587 22929-22931 CD denotes 15
T4588 22926-22928 CD denotes 10
T4589 22928-22929 , denotes ,
T4590 22931-22932 -RRB- denotes ]
T4591 22932-22933 . denotes .
T4592 22933-23036 sentence denotes It is noteworthy that cathepsin B and the CLN2-encoded enzyme, TPPI, are also altered in JNCL [18-20].
T4593 22934-22936 PRP denotes It
T4594 22937-22939 VBZ denotes is
T4595 22940-22950 JJ denotes noteworthy
T4596 22951-22955 IN denotes that
T4597 23012-23019 VBN denotes altered
T4598 22956-22965 NN denotes cathepsin
T4599 22966-22967 NN denotes B
T4600 22968-22971 CC denotes and
T4601 22972-22975 DT denotes the
T4602 22989-22995 NN denotes enzyme
T4603 22976-22980 NN denotes CLN2
T4604 22981-22988 VBN denotes encoded
T4605 22980-22981 HYPH denotes -
T4606 22995-22997 , denotes ,
T4607 22997-23001 NN denotes TPPI
T4608 23001-23003 , denotes ,
T4609 23003-23006 VBP denotes are
T4610 23007-23011 RB denotes also
T4611 23020-23022 IN denotes in
T4612 23023-23027 NN denotes JNCL
T4613 23028-23029 -LRB- denotes [
T4614 23029-23031 CD denotes 18
T4615 23031-23032 SYM denotes -
T4616 23032-23034 CD denotes 20
T4617 23034-23035 -RRB- denotes ]
T4618 23035-23036 . denotes .
T4619 23036-23208 sentence denotes Nevertheless, despite the cathepsin D protein alterations that are observed in homozygous CbCln3Δex7/8 cells, cathepsin D enzymatic activity does not appear to be reduced.
T4621 23187-23193 VB denotes appear
T4622 23049-23051 , denotes ,
T4623 23051-23058 IN denotes despite
T4624 23059-23062 DT denotes the
T4625 23083-23094 NNS denotes alterations
T4626 23063-23072 NN denotes cathepsin
T4627 23073-23074 NN denotes D
T4628 23075-23082 NN denotes protein
T4629 23095-23099 WDT denotes that
T4630 23104-23112 VBN denotes observed
T4631 23100-23103 VBP denotes are
T4632 23113-23115 IN denotes in
T4633 23116-23126 JJ denotes homozygous
T4634 23140-23145 NNS denotes cells
T4635 23127-23137 NN denotes CbCln3Δex7
T4636 23137-23138 HYPH denotes /
T4637 23138-23139 CD denotes 8
T4638 23145-23147 , denotes ,
T4639 23147-23156 NN denotes cathepsin
T4640 23157-23158 NN denotes D
T4641 23169-23177 NN denotes activity
T4642 23159-23168 JJ denotes enzymatic
T4643 23178-23182 VBZ denotes does
T4644 23183-23186 RB denotes not
T4645 23194-23196 TO denotes to
T4646 23200-23207 VBN denotes reduced
T4647 23197-23199 VB denotes be
T4648 23207-23208 . denotes .
T4649 23208-23304 sentence denotes Thus, decreased cathepsin D activity is unlikely to account for subunit c accumulation in JNCL.
T4650 23209-23213 RB denotes Thus
T4651 23246-23248 VBZ denotes is
T4652 23213-23215 , denotes ,
T4653 23215-23224 VBN denotes decreased
T4654 23237-23245 NN denotes activity
T4655 23225-23234 NN denotes cathepsin
T4656 23235-23236 NN denotes D
T4657 23249-23257 JJ denotes unlikely
T4658 23258-23260 TO denotes to
T4659 23261-23268 VB denotes account
T4660 23269-23272 IN denotes for
T4661 23273-23280 NN denotes subunit
T4662 23281-23282 NN denotes c
T4663 23283-23295 NN denotes accumulation
T4664 23296-23298 IN denotes in
T4665 23299-23303 NN denotes JNCL
T4666 23303-23304 . denotes .
T4667 23304-23425 sentence denotes Aging of homozygous CbCln3Δex7/8 cells at confluency is necessary to induce significantly accumulated subunit c protein.
T4668 23305-23310 NN denotes Aging
T4669 23358-23360 VBZ denotes is
T4670 23311-23313 IN denotes of
T4671 23314-23324 JJ denotes homozygous
T4672 23338-23343 NNS denotes cells
T4673 23325-23335 NN denotes CbCln3Δex7
T4674 23335-23336 HYPH denotes /
T4675 23336-23337 CD denotes 8
T4676 23344-23346 IN denotes at
T4677 23347-23357 NN denotes confluency
T4678 23361-23370 JJ denotes necessary
T4679 23371-23373 TO denotes to
T4680 23374-23380 VB denotes induce
T4681 23381-23394 RB denotes significantly
T4682 23395-23406 VBN denotes accumulated
T4683 23417-23424 NN denotes protein
T4684 23407-23414 NN denotes subunit
T4685 23415-23416 NN denotes c
T4686 23424-23425 . denotes .
T4687 23425-23583 sentence denotes However, membrane organelle disruptions precede subunit c accumulation in homozygous CbCln3Δex7/8 cells, since they are observed without aging at confluency.
T4688 23426-23433 RB denotes However
T4689 23466-23473 VBP denotes precede
T4690 23433-23435 , denotes ,
T4691 23435-23443 NN denotes membrane
T4692 23444-23453 NN denotes organelle
T4693 23454-23465 NNS denotes disruptions
T4694 23474-23481 NN denotes subunit
T4695 23482-23483 NN denotes c
T4696 23484-23496 NN denotes accumulation
T4697 23497-23499 IN denotes in
T4698 23500-23510 JJ denotes homozygous
T4699 23524-23529 NNS denotes cells
T4700 23511-23521 NN denotes CbCln3Δex7
T4701 23521-23522 HYPH denotes /
T4702 23522-23523 CD denotes 8
T4703 23529-23531 , denotes ,
T4704 23531-23536 IN denotes since
T4705 23546-23554 VBN denotes observed
T4706 23537-23541 PRP denotes they
T4707 23542-23545 VBP denotes are
T4708 23555-23562 IN denotes without
T4709 23563-23568 VBG denotes aging
T4710 23569-23571 IN denotes at
T4711 23572-23582 NN denotes confluency
T4712 23582-23583 . denotes .
T4713 23583-23764 sentence denotes Lysosomal and endosomal size and distribution are altered, and mitochondria are abnormally elongated and functionally compromised in sub-confluent homozygous CbCln3Δex7/8 cultures.
T4714 23584-23593 JJ denotes Lysosomal
T4715 23608-23612 NN denotes size
T4716 23594-23597 CC denotes and
T4717 23598-23607 JJ denotes endosomal
T4718 23634-23641 VBN denotes altered
T4719 23613-23616 CC denotes and
T4720 23617-23629 NN denotes distribution
T4721 23630-23633 VBP denotes are
T4722 23641-23643 , denotes ,
T4723 23643-23646 CC denotes and
T4724 23647-23659 NNS denotes mitochondria
T4725 23660-23663 VBP denotes are
T4726 23664-23674 RB denotes abnormally
T4727 23675-23684 VBN denotes elongated
T4728 23685-23688 CC denotes and
T4729 23689-23701 RB denotes functionally
T4730 23702-23713 VBN denotes compromised
T4731 23714-23716 IN denotes in
T4732 23717-23730 JJ denotes sub-confluent
T4733 23755-23763 NNS denotes cultures
T4734 23731-23741 JJ denotes homozygous
T4735 23742-23752 NN denotes CbCln3Δex7
T4736 23752-23753 HYPH denotes /
T4737 23753-23754 CD denotes 8
T4738 23763-23764 . denotes .
T4739 23764-23987 sentence denotes These observations argue that membrane trafficking defects do not result from excessive subunit c accumulation compromising the lysosome, but rather are early events in the disease process preceding subunit c accumulation.
T4740 23765-23770 DT denotes These
T4741 23771-23783 NNS denotes observations
T4742 23784-23789 VBP denotes argue
T4743 23790-23794 IN denotes that
T4744 23831-23837 VB denotes result
T4745 23795-23803 NN denotes membrane
T4746 23804-23815 NN denotes trafficking
T4747 23816-23823 NNS denotes defects
T4748 23824-23826 VBP denotes do
T4749 23827-23830 RB denotes not
T4750 23838-23842 IN denotes from
T4751 23843-23852 JJ denotes excessive
T4752 23863-23875 NN denotes accumulation
T4753 23853-23860 NN denotes subunit
T4754 23861-23862 NN denotes c
T4755 23876-23888 VBG denotes compromising
T4756 23889-23892 DT denotes the
T4757 23893-23901 NN denotes lysosome
T4758 23901-23903 , denotes ,
T4759 23903-23906 CC denotes but
T4760 23907-23913 RB denotes rather
T4761 23914-23917 VBP denotes are
T4762 23918-23923 JJ denotes early
T4763 23924-23930 NNS denotes events
T4764 23931-23933 IN denotes in
T4765 23934-23937 DT denotes the
T4766 23946-23953 NN denotes process
T4767 23938-23945 NN denotes disease
T4768 23954-23963 VBG denotes preceding
T4769 23964-23971 NN denotes subunit
T4770 23972-23973 NN denotes c
T4771 23974-23986 NN denotes accumulation
T4772 23986-23987 . denotes .
T4773 23987-24182 sentence denotes Mitochondrial abnormalities, which have also been reported in JNCL patients and other animal models [21-23], may result from ineffective turnover by autophagy, a lysosomal-targeted pathway [24].
T4774 23988-24001 JJ denotes Mitochondrial
T4775 24002-24015 NNS denotes abnormalities
T4776 24101-24107 VB denotes result
T4777 24015-24017 , denotes ,
T4778 24017-24022 WDT denotes which
T4779 24038-24046 VBN denotes reported
T4780 24023-24027 VBP denotes have
T4781 24028-24032 RB denotes also
T4782 24033-24037 VBN denotes been
T4783 24047-24049 IN denotes in
T4784 24050-24054 NN denotes JNCL
T4785 24055-24063 NNS denotes patients
T4786 24064-24067 CC denotes and
T4787 24068-24073 JJ denotes other
T4788 24081-24087 NNS denotes models
T4789 24074-24080 NN denotes animal
T4790 24088-24089 -LRB- denotes [
T4791 24089-24091 CD denotes 21
T4792 24091-24092 SYM denotes -
T4793 24092-24094 CD denotes 23
T4794 24094-24095 -RRB- denotes ]
T4795 24095-24097 , denotes ,
T4796 24097-24100 MD denotes may
T4797 24108-24112 IN denotes from
T4798 24113-24124 JJ denotes ineffective
T4799 24125-24133 NN denotes turnover
T4800 24134-24136 IN denotes by
T4801 24137-24146 NN denotes autophagy
T4802 24146-24148 , denotes ,
T4803 24148-24149 DT denotes a
T4804 24169-24176 NN denotes pathway
T4805 24150-24159 JJ denotes lysosomal
T4806 24160-24168 VBN denotes targeted
T4807 24159-24160 HYPH denotes -
T4808 24177-24178 -LRB- denotes [
T4809 24178-24180 CD denotes 24
T4810 24180-24181 -RRB- denotes ]
T4811 24181-24182 . denotes .
T4812 24182-24394 sentence denotes Alternatively, or simultaneously, battenin deficiency may impact mitochondrial function upstream of turnover, affecting mitochondrial biogenesis and/or altered transport and processing of mitochondrial proteins.
T4813 24183-24196 RB denotes Alternatively
T4814 24241-24247 VB denotes impact
T4815 24196-24198 , denotes ,
T4816 24198-24200 CC denotes or
T4817 24201-24215 RB denotes simultaneously
T4818 24215-24217 , denotes ,
T4819 24217-24225 NN denotes battenin
T4820 24226-24236 NN denotes deficiency
T4821 24237-24240 MD denotes may
T4822 24248-24261 JJ denotes mitochondrial
T4823 24262-24270 NN denotes function
T4824 24271-24279 RB denotes upstream
T4825 24280-24282 IN denotes of
T4826 24283-24291 NN denotes turnover
T4827 24291-24293 , denotes ,
T4828 24293-24302 VBG denotes affecting
T4829 24303-24316 JJ denotes mitochondrial
T4830 24317-24327 NN denotes biogenesis
T4831 24328-24331 CC denotes and
T4832 24331-24332 HYPH denotes /
T4833 24332-24334 CC denotes or
T4834 24335-24342 VBN denotes altered
T4835 24343-24352 NN denotes transport
T4836 24353-24356 CC denotes and
T4837 24357-24367 NN denotes processing
T4838 24368-24370 IN denotes of
T4839 24371-24384 JJ denotes mitochondrial
T4840 24385-24393 NN denotes proteins
T4841 24393-24394 . denotes .
T4842 24394-24502 sentence denotes In wild-type CbCln3 neuronal precursor cells battenin primarily co-localizes with early and late endosomes.
T4843 24395-24397 IN denotes In
T4844 24459-24471 VBZ denotes co-localizes
T4845 24398-24402 JJ denotes wild
T4846 24403-24407 NN denotes type
T4847 24402-24403 HYPH denotes -
T4848 24434-24439 NNS denotes cells
T4849 24408-24414 NN denotes CbCln3
T4850 24415-24423 JJ denotes neuronal
T4904 24838-24846 VBN denotes reported
T4905 24779-24784 JJ denotes other
T4851 24424-24433 NN denotes precursor
T4852 24440-24448 NN denotes battenin
T4853 24449-24458 RB denotes primarily
T4854 24472-24476 IN denotes with
T4855 24477-24482 JJ denotes early
T4856 24492-24501 NNS denotes endosomes
T4857 24483-24486 CC denotes and
T4858 24487-24491 JJ denotes late
T4859 24501-24502 . denotes .
T4860 24502-24775 sentence denotes Battenin immunostaining in homozygous CbCln3Δex7/8 neuronal precursors is significantly reduced in abundance, but mutant signal also co-localizes with endosomal markers suggesting mutant battenin protein with C-terminal epitopes is trafficked similar to wild-type protein.
T4861 24503-24511 NN denotes Battenin
T4862 24512-24526 NN denotes immunostaining
T4863 24591-24598 VBN denotes reduced
T4864 24527-24529 IN denotes in
T4865 24530-24540 JJ denotes homozygous
T4866 24563-24573 NNS denotes precursors
T4867 24541-24551 NN denotes CbCln3Δex7
T4868 24551-24552 HYPH denotes /
T4869 24552-24553 CD denotes 8
T4870 24554-24562 JJ denotes neuronal
T4871 24574-24576 VBZ denotes is
T4872 24577-24590 RB denotes significantly
T4873 24599-24601 IN denotes in
T4874 24602-24611 NN denotes abundance
T4875 24611-24613 , denotes ,
T4876 24613-24616 CC denotes but
T4877 24617-24623 NN denotes mutant
T4878 24624-24630 NN denotes signal
T4879 24636-24648 VBZ denotes co-localizes
T4880 24631-24635 RB denotes also
T4881 24649-24653 IN denotes with
T4882 24654-24663 JJ denotes endosomal
T4883 24664-24671 NNS denotes markers
T4884 24672-24682 VBG denotes suggesting
T4885 24683-24689 NN denotes mutant
T4886 24699-24706 NN denotes protein
T4887 24690-24698 NN denotes battenin
T4888 24735-24745 VBN denotes trafficked
T4889 24707-24711 IN denotes with
T4890 24712-24713 NN denotes C
T4891 24714-24722 JJ denotes terminal
T4892 24713-24714 HYPH denotes -
T4893 24723-24731 NNS denotes epitopes
T4894 24732-24734 VBZ denotes is
T4895 24746-24753 JJ denotes similar
T4896 24754-24756 IN denotes to
T4897 24757-24761 JJ denotes wild
T4898 24762-24766 NN denotes type
T4899 24761-24762 HYPH denotes -
T4900 24767-24774 NN denotes protein
T4901 24774-24775 . denotes .
T4902 24775-24996 sentence denotes In other studies, CLN3/battenin protein localization has been reported to partially overlap with late endosomes and lysosomes in non-neuronal cells [7], and to lysosomes, synaptosomes [8] and endosomes [9,25] in neurons.
T4903 24776-24778 IN denotes In
T4906 24785-24792 NNS denotes studies
T4907 24792-24794 , denotes ,
T4908 24794-24798 NN denotes CLN3
T4909 24816-24828 NN denotes localization
T4910 24798-24799 HYPH denotes /
T4911 24799-24807 NN denotes battenin
T4912 24808-24815 NN denotes protein
T4913 24829-24832 VBZ denotes has
T4914 24833-24837 VBN denotes been
T4915 24847-24849 TO denotes to
T4916 24860-24867 VB denotes overlap
T4917 24850-24859 RB denotes partially
T4918 24868-24872 IN denotes with
T4919 24873-24877 JJ denotes late
T4920 24878-24887 NNS denotes endosomes
T4921 24888-24891 CC denotes and
T4922 24892-24901 NNS denotes lysosomes
T4923 24902-24904 IN denotes in
T4924 24905-24917 JJ denotes non-neuronal
T4925 24918-24923 NNS denotes cells
T4926 24924-24925 -LRB- denotes [
T4927 24925-24926 CD denotes 7
T4928 24926-24927 -RRB- denotes ]
T4929 24927-24929 , denotes ,
T4930 24929-24932 CC denotes and
T4931 24933-24935 IN denotes to
T4932 24936-24945 NNS denotes lysosomes
T4933 24945-24947 , denotes ,
T4934 24947-24959 NNS denotes synaptosomes
T4935 24960-24961 -LRB- denotes [
T4936 24961-24962 CD denotes 8
T4937 24962-24963 -RRB- denotes ]
T4938 24964-24967 CC denotes and
T4939 24968-24977 NNS denotes endosomes
T4940 24978-24979 -LRB- denotes [
T4941 24981-24983 CD denotes 25
T4942 24979-24980 CD denotes 9
T4943 24980-24981 , denotes ,
T4944 24983-24984 -RRB- denotes ]
T4971 25156-25165 JJ denotes vesicular
T4972 25166-25175 NN denotes transport
T4973 25176-25179 CC denotes and
T4974 25179-25180 HYPH denotes /
T4975 25180-25182 CC denotes or
T4976 25183-25189 NN denotes fusion
T4977 25189-25190 . denotes .
T4978 25190-25311 sentence denotes Endocytic and lysosomal-targeted pathways, including mitochondrial autophagy, are specifically implicated in this study.
T4979 25191-25200 JJ denotes Endocytic
T4980 25215-25223 VBN denotes targeted
T4981 25201-25204 CC denotes and
T4982 25205-25214 JJ denotes lysosomal
T4983 25214-25215 HYPH denotes -
T4984 25224-25232 NNS denotes pathways
T4985 25286-25296 VBN denotes implicated
T4986 25232-25234 , denotes ,
T4987 25234-25243 VBG denotes including
T4988 25244-25257 JJ denotes mitochondrial
T4989 25258-25267 NN denotes autophagy
T4990 25267-25269 , denotes ,
T4991 25269-25272 VBP denotes are
T4992 25273-25285 RB denotes specifically
T4993 25297-25299 IN denotes in
T4994 25300-25304 DT denotes this
T4995 25305-25310 NN denotes study
T4996 25310-25311 . denotes .
T5075 25526-25535 NN denotes transport
T5076 25535-25537 , denotes ,
T5077 25537-25540 CC denotes and
T5078 25541-25542 DT denotes a
T5079 25555-25565 NN denotes metabolism
T5080 25543-25547 JJ denotes high
T5081 25548-25554 NN denotes energy
T5082 25547-25548 HYPH denotes -
T5083 25578-25587 VB denotes sensitize
T5084 25566-25569 MD denotes may
T5085 25570-25577 RB denotes further
T5086 25588-25595 NNS denotes neurons
T5087 25596-25598 IN denotes to
T5088 25599-25602 DT denotes the
T5089 25603-25607 NN denotes loss
T5090 25608-25610 IN denotes of
T5091 25611-25619 NN denotes battenin
T5092 25620-25628 NN denotes activity
T5093 25628-25629 . denotes .
T5094 25629-25810 sentence denotes Thus, our panel of wild-type, heterozygous, and homozygous CbCln3Δex7/8 cerebellar cells provide an ideal model system to further elucidate battenin function and JNCL pathogenesis.
T5095 25630-25634 RB denotes Thus
T5096 25719-25726 VBP denotes provide
T5097 25634-25636 , denotes ,
T5098 25636-25639 PRP$ denotes our
T5099 25640-25645 NN denotes panel
T5100 25646-25648 IN denotes of
T5101 25649-25653 JJ denotes wild
T5102 25654-25658 NN denotes type
T5103 25653-25654 HYPH denotes -
T5104 25713-25718 NNS denotes cells
T5105 25658-25660 , denotes ,
T5106 25660-25672 JJ denotes heterozygous
T5107 25672-25674 , denotes ,
T5108 25674-25677 CC denotes and
T5109 25678-25688 JJ denotes homozygous
T5110 25689-25699 NN denotes CbCln3Δex7
T5111 25699-25700 HYPH denotes /
T5112 25700-25701 CD denotes 8
T5113 25702-25712 JJ denotes cerebellar
T5114 25727-25729 DT denotes an
T5115 25742-25748 NN denotes system
T5116 25730-25735 JJ denotes ideal
T5117 25736-25741 NN denotes model
T5118 25749-25751 TO denotes to
T5119 25760-25769 VB denotes elucidate
T5120 25752-25759 RBR denotes further
T5121 25770-25778 NN denotes battenin
T5122 25779-25787 NN denotes function
T5123 25788-25791 CC denotes and
T5124 25792-25796 NN denotes JNCL
T5125 25797-25809 NN denotes pathogenesis
T5126 25809-25810 . denotes .
T5236 25821-25829 NN denotes Antibody
T5237 25848-25856 NNS denotes reagents
T5238 25830-25833 CC denotes and
T5239 25834-25838 NN denotes cell
T5240 25839-25847 NN denotes staining
T5241 25856-26110 sentence denotes Nestin (clone Rat 401, 2 μg/ml), Lamp 1 (clone 1D4B, 6 μg/ml), and Lamp 2 (clone Abl-93; 6 μg/ml) monoclonal antibodies were obtained from the Developmental Studies Hybridoma Bank, maintained by The University of Iowa, Department of Biological Sciences.
T5242 25857-25863 NN denotes Nestin
T5243 25966-25976 NNS denotes antibodies
T5244 25864-25865 -LRB- denotes (
T5245 25882-25884 NN denotes μg
T5246 25865-25870 NN denotes clone
T5247 25871-25874 NN denotes Rat
T5248 25875-25878 CD denotes 401
T5249 25878-25880 , denotes ,
T5250 25880-25881 CD denotes 2
T5251 25884-25885 SYM denotes /
T5252 25885-25887 NN denotes ml
T5253 25887-25888 -RRB- denotes )
T5254 25888-25890 , denotes ,
T5255 25890-25894 NN denotes Lamp
T5256 25895-25896 CD denotes 1
T5257 25897-25898 -LRB- denotes (
T5258 25912-25914 NN denotes μg
T5259 25898-25903 NN denotes clone
T5260 25904-25908 NN denotes 1D4B
T5261 25908-25910 , denotes ,
T5262 25910-25911 CD denotes 6
T5263 25914-25915 SYM denotes /
T5264 25915-25917 NN denotes ml
T5265 25917-25918 -RRB- denotes )
T5266 25918-25920 , denotes ,
T5267 25920-25923 CC denotes and
T5268 25924-25928 NN denotes Lamp
T5269 25929-25930 CD denotes 2
T5270 25931-25932 -LRB- denotes (
T5271 25948-25950 NN denotes μg
T5272 25932-25937 NN denotes clone
T5273 25938-25941 NN denotes Abl
T5274 25941-25942 HYPH denotes -
T5275 25942-25944 CD denotes 93
T5276 25944-25945 : denotes ;
T5277 25946-25947 CD denotes 6
T5278 25950-25951 SYM denotes /
T5279 25951-25953 NN denotes ml
T5280 25953-25954 -RRB- denotes )
T5281 25955-25965 JJ denotes monoclonal
T5282 25982-25990 VBN denotes obtained
T5283 25977-25981 VBD denotes were
T5284 25991-25995 IN denotes from
T5285 25996-25999 DT denotes the
T5286 26032-26036 NNP denotes Bank
T5287 26000-26013 NNP denotes Developmental
T5288 26014-26021 NNPS denotes Studies
T5289 26022-26031 NNP denotes Hybridoma
T5290 26036-26038 , denotes ,
T5291 26038-26048 VBN denotes maintained
T5292 26049-26051 IN denotes by
T5293 26052-26055 DT denotes The
T5294 26056-26066 NNP denotes University
T5295 26076-26086 NNP denotes Department
T5296 26067-26069 IN denotes of
T5297 26070-26074 NNP denotes Iowa
T5298 26074-26076 , denotes ,
T5299 26087-26089 IN denotes of
T5300 26090-26100 NNP denotes Biological
T5301 26101-26109 NNPS denotes Sciences
T5302 26109-26110 . denotes .
T5303 26110-26157 sentence denotes Batp1 (1 μg/ml) was previously described [12].
T5304 26111-26116 NN denotes Batp1
T5305 26142-26151 VBN denotes described
T5306 26117-26118 -LRB- denotes (
T5307 26120-26122 NN denotes μg
T5308 26118-26119 CD denotes 1
T5309 26122-26123 SYM denotes /
T5310 26123-26125 NN denotes ml
T5311 26125-26126 -RRB- denotes )
T5312 26127-26130 VBD denotes was
T5313 26131-26141 RB denotes previously
T5314 26152-26153 -LRB- denotes [
T5315 26153-26155 CD denotes 12
T5316 26155-26156 -RRB- denotes ]
T5317 26156-26157 . denotes .
T5318 26157-26266 sentence denotes Anti-subunit c antibody (0.7 μg/ml) was kindly provided by Dr. Kominami (Juntendo University, Tokyo, Japan).
T5319 26158-26170 JJ denotes Anti-subunit
T5320 26171-26172 NN denotes c
T5321 26173-26181 NN denotes antibody
T5322 26205-26213 VBN denotes provided
T5323 26182-26183 -LRB- denotes (
T5324 26187-26189 NN denotes μg
T5325 26183-26186 CD denotes 0.7
T5326 26189-26190 SYM denotes /
T5327 26190-26192 NN denotes ml
T5328 26192-26193 -RRB- denotes )
T5329 26194-26197 VBD denotes was
T5330 26198-26204 RB denotes kindly
T5331 26214-26216 IN denotes by
T5332 26217-26220 NNP denotes Dr.
T5333 26221-26229 NNP denotes Kominami
T5334 26230-26231 -LRB- denotes (
T5335 26240-26250 NNP denotes University
T5336 26231-26239 NNP denotes Juntendo
T5337 26250-26252 , denotes ,
T5338 26252-26257 NNP denotes Tokyo
T5339 26257-26259 , denotes ,
T5340 26259-26264 NNP denotes Japan
T5341 26264-26265 -RRB- denotes )
T5342 26265-26266 . denotes .
T5343 26266-26839 sentence denotes Additional antibodies were as follows: GFAP, 1:2000 (DAKO Corporation); calbindin, 1:5000 (Sigma); NeuN, 10 μg/ml (Chemicon); SV40 T antigen (Pab 101), 2 μg/ml (Santa Cruz Biotechnology); cathepsin D (C-20), 2 μg/ml (Santa Cruz Biotechnology); cytochrome c oxidase subunit IV (cox4), 1:1000 (BD Biosciences Clontech); PDI (H-160), 2 μg/ml (Santa Cruz Biotechnology); GM130, 1 μg/ml (BD Transduction Labs); α-tubulin, 1:15,000 (Sigma); grp75, 1:200 (Stressgen); early endosome antigen-1 (EEA1), 2 μg/ml (Santa Cruz Biotechnology); rab 7, 4 μg/ml (Santa Cruz Biotechnology).
T5344 26267-26277 JJ denotes Additional
T5345 26278-26288 NNS denotes antibodies
T5346 26289-26293 VBD denotes were
T5347 26294-26296 IN denotes as
T5348 26297-26304 VBZ denotes follows
T5349 26304-26306 : denotes :
T5350 26306-26310 NN denotes GFAP
T5351 26310-26312 , denotes ,
T5352 26312-26313 CD denotes 1
T5353 26313-26314 SYM denotes :
T5354 26314-26318 CD denotes 2000
T5355 26319-26320 -LRB- denotes (
T5356 26325-26336 NN denotes Corporation
T5357 26320-26324 NN denotes DAKO
T5358 26336-26337 -RRB- denotes )
T5359 26337-26338 : denotes ;
T5360 26339-26348 NN denotes calbindin
T5361 26348-26350 , denotes ,
T5362 26350-26351 CD denotes 1
T5363 26351-26352 SYM denotes :
T5364 26352-26356 CD denotes 5000
T5365 26357-26358 -LRB- denotes (
T5366 26358-26363 NNP denotes Sigma
T5367 26363-26364 -RRB- denotes )
T5368 26364-26365 : denotes ;
T5369 26366-26370 NN denotes NeuN
T5370 26370-26372 , denotes ,
T5371 26372-26374 CD denotes 10
T5372 26375-26377 NN denotes μg
T5373 26377-26378 SYM denotes /
T5374 26378-26380 NN denotes ml
T5375 26381-26382 -LRB- denotes (
T5376 26382-26390 NNP denotes Chemicon
T5377 26390-26391 -RRB- denotes )
T5378 26391-26392 : denotes ;
T5379 26393-26397 NN denotes SV40
T5380 26400-26407 NN denotes antigen
T5381 26398-26399 NN denotes T
T5382 26408-26409 -LRB- denotes (
T5383 26409-26412 NN denotes Pab
T5384 26413-26416 CD denotes 101
T5385 26416-26417 -RRB- denotes )
T5386 26417-26419 , denotes ,
T5387 26419-26420 CD denotes 2
T5388 26421-26423 NN denotes μg
T5389 26423-26424 SYM denotes /
T5390 26424-26426 NN denotes ml
T5391 26427-26428 -LRB- denotes (
T5392 26439-26452 NNP denotes Biotechnology
T5393 26428-26433 NNP denotes Santa
T5394 26434-26438 NNP denotes Cruz
T5395 26452-26453 -RRB- denotes )
T5396 26453-26454 : denotes ;
T5397 26455-26464 NN denotes cathepsin
T5398 26465-26466 NN denotes D
T5399 26467-26468 -LRB- denotes (
T5400 26468-26469 NN denotes C
T5401 26469-26470 HYPH denotes -
T5402 26470-26472 CD denotes 20
T5403 26472-26473 -RRB- denotes )
T5404 26473-26475 , denotes ,
T5405 26475-26476 CD denotes 2
T5406 26477-26479 NN denotes μg
T5407 26479-26480 SYM denotes /
T5408 26480-26482 NN denotes ml
T5409 26483-26484 -LRB- denotes (
T5410 26495-26508 NNP denotes Biotechnology
T5411 26484-26489 NNP denotes Santa
T5412 26490-26494 NNP denotes Cruz
T5413 26508-26509 -RRB- denotes )
T5414 26509-26510 : denotes ;
T5415 26511-26521 NN denotes cytochrome
T5416 26532-26539 NN denotes subunit
T5417 26522-26523 NN denotes c
T5418 26524-26531 NN denotes oxidase
T5419 26540-26542 CD denotes IV
T5420 26543-26544 -LRB- denotes (
T5421 26544-26548 NN denotes cox4
T5422 26548-26549 -RRB- denotes )
T5423 26549-26551 , denotes ,
T5424 26551-26552 CD denotes 1
T5425 26552-26553 SYM denotes :
T5426 26553-26557 CD denotes 1000
T5427 26558-26559 -LRB- denotes (
T5428 26574-26582 NNP denotes Clontech
T5429 26559-26561 NN denotes BD
T5430 26562-26573 NNP denotes Biosciences
T5431 26582-26583 -RRB- denotes )
T5432 26583-26584 : denotes ;
T5433 26585-26588 NN denotes PDI
T5434 26589-26590 -LRB- denotes (
T5435 26590-26591 NN denotes H
T5436 26591-26592 HYPH denotes -
T5437 26592-26595 CD denotes 160
T5438 26595-26596 -RRB- denotes )
T5439 26596-26598 , denotes ,
T5440 26598-26599 CD denotes 2
T5441 26600-26602 NN denotes μg
T5442 26602-26603 SYM denotes /
T5443 26603-26605 NN denotes ml
T5444 26606-26607 -LRB- denotes (
T5445 26618-26631 NNP denotes Biotechnology
T5446 26607-26612 NNP denotes Santa
T5447 26613-26617 NNP denotes Cruz
T5448 26631-26632 -RRB- denotes )
T5449 26632-26633 : denotes ;
T5450 26634-26639 NN denotes GM130
T5451 26639-26641 , denotes ,
T5452 26641-26642 CD denotes 1
T5453 26643-26645 NN denotes μg
T5454 26645-26646 SYM denotes /
T5455 26646-26648 NN denotes ml
T5456 26649-26650 -LRB- denotes (
T5457 26666-26670 NNS denotes Labs
T5458 26650-26652 NN denotes BD
T5459 26653-26665 NN denotes Transduction
T5460 26670-26671 -RRB- denotes )
T5461 26671-26672 : denotes ;
T5462 26673-26674 NN denotes α
T5463 26675-26682 NN denotes tubulin
T5464 26674-26675 HYPH denotes -
T5465 26682-26684 , denotes ,
T5466 26684-26685 CD denotes 1
T5467 26685-26686 SYM denotes :
T5468 26686-26692 CD denotes 15,000
T5469 26693-26694 -LRB- denotes (
T5470 26694-26699 NNP denotes Sigma
T5471 26699-26700 -RRB- denotes )
T5472 26700-26701 : denotes ;
T5473 26702-26707 NN denotes grp75
T5474 26707-26709 , denotes ,
T5475 26709-26710 CD denotes 1
T5476 26710-26711 SYM denotes :
T5477 26711-26714 CD denotes 200
T5478 26715-26716 -LRB- denotes (
T5479 26716-26725 NNP denotes Stressgen
T5480 26725-26726 -RRB- denotes )
T5481 26726-26727 : denotes ;
T5482 26728-26733 JJ denotes early
T5483 26743-26750 NN denotes antigen
T5484 26734-26742 NN denotes endosome
T5485 26750-26751 HYPH denotes -
T5486 26751-26752 CD denotes 1
T5487 26753-26754 -LRB- denotes (
T5488 26754-26758 NN denotes EEA1
T5489 26758-26759 -RRB- denotes )
T5490 26759-26761 , denotes ,
T5491 26761-26762 CD denotes 2
T5492 26763-26765 NN denotes μg
T5493 26765-26766 SYM denotes /
T5494 26766-26768 NN denotes ml
T5495 26769-26770 -LRB- denotes (
T5496 26781-26794 NNP denotes Biotechnology
T5497 26770-26775 NNP denotes Santa
T5498 26776-26780 NNP denotes Cruz
T5499 26794-26795 -RRB- denotes )
T5500 26795-26796 : denotes ;
T5501 26797-26800 NN denotes rab
T5502 26801-26802 CD denotes 7
T5503 26802-26804 , denotes ,
T5504 26804-26805 CD denotes 4
T5505 26806-26808 NN denotes μg
T5506 26808-26809 SYM denotes /
T5507 26809-26811 NN denotes ml
T5508 26812-26813 -LRB- denotes (
T5509 26824-26837 NNP denotes Biotechnology
T5510 26813-26818 NNP denotes Santa
T5511 26819-26823 NNP denotes Cruz
T5512 26837-26838 -RRB- denotes )
T5513 26838-26839 . denotes .
T5514 26839-27012 sentence denotes All fluorescent secondary antibodies were obtained from Jackson ImmunoResearch Laboratories and HRP-conjugated secondary antibodies were obtained from Amersham Biosciences.
T5515 26840-26843 DT denotes All
T5516 26866-26876 NNS denotes antibodies
T5517 26844-26855 JJ denotes fluorescent
T5518 26856-26865 JJ denotes secondary
T5519 26882-26890 VBN denotes obtained
T5520 26877-26881 VBD denotes were
T5521 26891-26895 IN denotes from
T5522 26896-26903 NNP denotes Jackson
T5523 26919-26931 NNP denotes Laboratories
T5524 26904-26918 NNP denotes ImmunoResearch
T5525 26932-26935 CC denotes and
T5526 26936-26939 NN denotes HRP
T5527 26940-26950 VBN denotes conjugated
T5528 26939-26940 HYPH denotes -
T5529 26961-26971 NNS denotes antibodies
T5530 26951-26960 JJ denotes secondary
T5531 26977-26985 VBN denotes obtained
T5532 26972-26976 VBD denotes were
T5533 26986-26990 IN denotes from
T5534 26991-26999 NNP denotes Amersham
T5535 27000-27011 NNP denotes Biosciences
T5536 27011-27012 . denotes .
T5537 27012-27182 sentence denotes Additional cell markers were as follows: VVL-biotin, 1:2000 (Vector Laboratories), 10,000 MW dextran-FITC, 1 mg/ml and Lysotracker Red DND-99, 500 nM (Molecular Probes).
T5538 27013-27023 JJ denotes Additional
T5539 27029-27036 NNS denotes markers
T5540 27024-27028 NN denotes cell
T5541 27037-27041 VBD denotes were
T5542 27042-27044 IN denotes as
T5543 27045-27052 VBZ denotes follows
T5544 27052-27054 : denotes :
T5545 27054-27057 NN denotes VVL
T5546 27058-27064 NN denotes biotin
T5547 27057-27058 HYPH denotes -
T5548 27064-27066 , denotes ,
T5549 27066-27067 CD denotes 1
T5550 27067-27068 SYM denotes :
T5551 27068-27072 CD denotes 2000
T5552 27073-27074 -LRB- denotes (
T5553 27081-27093 NNP denotes Laboratories
T5554 27074-27080 NNP denotes Vector
T5555 27093-27094 -RRB- denotes )
T5556 27094-27096 , denotes ,
T5557 27096-27102 CD denotes 10,000
T5558 27103-27105 NN denotes MW
T5559 27114-27118 NN denotes FITC
T5560 27106-27113 NN denotes dextran
T5561 27113-27114 HYPH denotes -
T5562 27118-27120 , denotes ,
T5563 27120-27121 CD denotes 1
T5564 27122-27124 NN denotes mg
T5565 27124-27125 SYM denotes /
T5566 27125-27127 NN denotes ml
T5567 27128-27131 CC denotes and
T5568 27132-27143 NNP denotes Lysotracker
T5569 27148-27151 NN denotes DND
T5570 27144-27147 NN denotes Red
T5571 27151-27152 HYPH denotes -
T5572 27152-27154 CD denotes 99
T5573 27154-27156 , denotes ,
T5574 27156-27159 CD denotes 500
T5575 27160-27162 NN denotes nM
T5576 27163-27164 -LRB- denotes (
T5577 27174-27180 NNP denotes Probes
T5578 27164-27173 NNP denotes Molecular
T5579 27180-27181 -RRB- denotes )
T5580 27181-27182 . denotes .
T5720 27268-27273 NNS denotes lines
T5713 27184-27194 NN denotes Generation
T5714 27194-27196 , denotes ,
T5715 27196-27207 NN denotes maintenance
T5716 27208-27211 CC denotes and
T5717 27212-27227 NN denotes differentiation
T5718 27228-27230 IN denotes of
T5719 27231-27237 NN denotes CbCln3
T5721 27238-27248 JJ denotes cerebellar
T5722 27249-27257 JJ denotes neuronal
T5723 27258-27267 NN denotes precursor
T5724 27273-27335 sentence denotes Cln3Δex7/8 knock-in mice have been previously described [12].
T5725 27274-27282 NN denotes Cln3Δex7
T5726 27294-27298 NNS denotes mice
T5727 27282-27283 HYPH denotes /
T5728 27283-27284 CD denotes 8
T5729 27285-27290 VB denotes knock
T5730 27290-27291 HYPH denotes -
T5731 27291-27293 RP denotes in
T5732 27320-27329 VBN denotes described
T5733 27299-27303 VBP denotes have
T5734 27304-27308 VBN denotes been
T5735 27309-27319 RB denotes previously
T5736 27330-27331 -LRB- denotes [
T5737 27331-27333 CD denotes 12
T5738 27333-27334 -RRB- denotes ]
T5739 27334-27335 . denotes .
T5740 27335-27515 sentence denotes Littermate pups from heterozygote crosses were used for primary culture establishment, by previously described methods that yield cerebellar granule neuron-enriched cultures [26].
T5741 27336-27346 NN denotes Littermate
T5742 27347-27351 NNS denotes pups
T5743 27383-27387 VBN denotes used
T5744 27352-27356 IN denotes from
T5745 27357-27369 NN denotes heterozygote
T5746 27370-27377 NNS denotes crosses
T5747 27378-27382 VBD denotes were
T5748 27388-27391 IN denotes for
T5749 27392-27399 JJ denotes primary
T5750 27408-27421 NN denotes establishment
T5751 27400-27407 NN denotes culture
T5752 27421-27423 , denotes ,
T5753 27423-27425 IN denotes by
T5754 27426-27436 RB denotes previously
T5755 27437-27446 VBN denotes described
T5756 27447-27454 NNS denotes methods
T5757 27455-27459 WDT denotes that
T5758 27460-27465 VBP denotes yield
T5759 27466-27476 JJ denotes cerebellar
T5760 27485-27491 NN denotes neuron
T5761 27477-27484 NN denotes granule
T5762 27492-27500 VBN denotes enriched
T5763 27491-27492 HYPH denotes -
T5764 27501-27509 NNS denotes cultures
T5765 27510-27511 -LRB- denotes [
T5766 27511-27513 CD denotes 26
T5767 27513-27514 -RRB- denotes ]
T5768 27514-27515 . denotes .
T5769 27515-27642 sentence denotes Postnatal day 4 (P4) cerebella were dissected in Hank's Balanced Salt Solution (HBSS, Sigma), supplemented with 35 mM glucose.
T5770 27516-27525 JJ denotes Postnatal
T5771 27526-27529 NN denotes day
T5772 27537-27546 NNS denotes cerebella
T5773 27530-27531 CD denotes 4
T5774 27532-27533 -LRB- denotes (
T5775 27533-27535 NN denotes P4
T5776 27535-27536 -RRB- denotes )
T5777 27552-27561 VBN denotes dissected
T5778 27547-27551 VBD denotes were
T5779 27562-27564 IN denotes in
T5780 27565-27569 NNP denotes Hank
T5781 27586-27594 NNP denotes Solution
T5782 27569-27571 POS denotes 's
T5783 27572-27580 NNP denotes Balanced
T5784 27581-27585 NNP denotes Salt
T5785 27595-27596 -LRB- denotes (
T5786 27596-27600 NN denotes HBSS
T5787 27600-27602 , denotes ,
T5788 27602-27607 NNP denotes Sigma
T5789 27607-27608 -RRB- denotes )
T5790 27608-27610 , denotes ,
T5791 27610-27622 VBN denotes supplemented
T5792 27623-27627 IN denotes with
T5793 27628-27630 CD denotes 35
T5794 27631-27633 NN denotes mM
T5795 27634-27641 NN denotes glucose
T5796 27641-27642 . denotes .
T5797 27642-27726 sentence denotes Tail biopsies were also collected for genomic DNA isolation and genotypic analysis.
T5798 27643-27647 NN denotes Tail
T5799 27648-27656 NNS denotes biopsies
T5800 27667-27676 VBN denotes collected
T5801 27657-27661 VBD denotes were
T5802 27662-27666 RB denotes also
T5803 27677-27680 IN denotes for
T5804 27681-27688 JJ denotes genomic
T5805 27693-27702 NN denotes isolation
T5806 27689-27692 NN denotes DNA
T5807 27703-27706 CC denotes and
T5808 27707-27716 JJ denotes genotypic
T5809 27717-27725 NN denotes analysis
T5810 27725-27726 . denotes .
T5811 27726-27920 sentence denotes Trypsin/EDTA (10 mg/ml, Sigma) and DNase I (100 μg/ml, Sigma), suspended in HBSS, helped dissociate cerebella for primary culture plating onto 0.01% poly-ornithine (Sigma) coated 100 mm dishes.
T5812 27727-27734 NN denotes Trypsin
T5813 27735-27739 NN denotes EDTA
T5814 27734-27735 HYPH denotes /
T5815 27809-27815 VBD denotes helped
T5816 27740-27741 -LRB- denotes (
T5817 27751-27756 NNP denotes Sigma
T5818 27741-27743 CD denotes 10
T5819 27744-27746 NN denotes mg
T5820 27746-27747 SYM denotes /
T5821 27747-27749 NN denotes ml
T5822 27749-27751 , denotes ,
T5823 27756-27757 -RRB- denotes )
T5824 27758-27761 CC denotes and
T5825 27762-27767 NN denotes DNase
T5826 27768-27769 CD denotes I
T5827 27770-27771 -LRB- denotes (
T5828 27782-27787 NNP denotes Sigma
T5829 27771-27774 CD denotes 100
T5830 27775-27777 NN denotes μg
T5831 27777-27778 SYM denotes /
T5832 27778-27780 NN denotes ml
T5833 27780-27782 , denotes ,
T5834 27787-27788 -RRB- denotes )
T5835 27788-27790 , denotes ,
T5836 27790-27799 VBN denotes suspended
T5837 27800-27802 IN denotes in
T5838 27803-27807 NN denotes HBSS
T5839 27807-27809 , denotes ,
T5840 27816-27826 VB denotes dissociate
T5841 27827-27836 NNS denotes cerebella
T5842 27837-27840 IN denotes for
T5843 27841-27848 JJ denotes primary
T5844 27849-27856 NN denotes culture
T5845 27857-27864 NN denotes plating
T5846 27865-27869 IN denotes onto
T5847 27870-27874 CD denotes 0.01
T5848 27874-27875 NN denotes %
T5849 27876-27890 NN denotes poly-ornithine
T5850 27899-27905 VBN denotes coated
T5851 27891-27892 -LRB- denotes (
T5852 27892-27897 NNP denotes Sigma
T5853 27897-27898 -RRB- denotes )
T5854 27913-27919 NNS denotes dishes
T5855 27906-27909 CD denotes 100
T5856 27910-27912 NN denotes mm
T5857 27919-27920 . denotes .
T5858 27920-28169 sentence denotes Primary cultures from individual cerebella were cultured overnight at 37°C, 5% CO2, in granule neuron growth media (Dulbecco's Modified Eagle Medium [DMEM, Gibco BRL #11995-065], 10% fetal bovine serum [Sigma #F-2442], supplemented with 24 mM KCl).
T5859 27921-27928 JJ denotes Primary
T5860 27929-27937 NNS denotes cultures
T5861 27969-27977 VBN denotes cultured
T5862 27938-27942 IN denotes from
T5863 27943-27953 JJ denotes individual
T5864 27954-27963 NNS denotes cerebella
T5865 27964-27968 VBD denotes were
T5866 27978-27987 RB denotes overnight
T5867 27988-27990 IN denotes at
T5868 27991-27993 CD denotes 37
T5869 27993-27995 NN denotes °C
T5870 27995-27997 , denotes ,
T5871 27997-27998 CD denotes 5
T5872 27998-27999 NN denotes %
T5873 28000-28003 NN denotes CO2
T5874 28003-28005 , denotes ,
T5875 28005-28007 IN denotes in
T5876 28008-28015 NN denotes granule
T5877 28016-28022 NN denotes neuron
T5878 28030-28035 NNS denotes media
T5879 28023-28029 NN denotes growth
T5880 28036-28037 -LRB- denotes (
T5881 28037-28045 NNP denotes Dulbecco
T5882 28063-28069 NNP denotes Medium
T5883 28045-28047 POS denotes 's
T5884 28048-28056 NNP denotes Modified
T5885 28057-28062 NNP denotes Eagle
T5886 28070-28071 -LRB- denotes [
T5887 28071-28075 NN denotes DMEM
T5888 28075-28077 , denotes ,
T5889 28077-28082 NNP denotes Gibco
T5890 28087-28088 NN denotes #
T5891 28083-28086 NNP denotes BRL
T5892 28088-28093 CD denotes 11995
T5893 28093-28094 HYPH denotes -
T5894 28094-28097 CD denotes 065
T5895 28097-28098 -RRB- denotes ]
T5896 28098-28100 , denotes ,
T5897 28100-28102 CD denotes 10
T5898 28102-28103 NN denotes %
T5899 28104-28109 JJ denotes fetal
T5900 28117-28122 NN denotes serum
T5901 28110-28116 JJ denotes bovine
T5902 28123-28124 -LRB- denotes [
T5903 28124-28129 NNP denotes Sigma
T5904 28131-28132 NN denotes F
T5905 28130-28131 NN denotes #
T5906 28132-28133 HYPH denotes -
T5907 28133-28137 CD denotes 2442
T5908 28137-28138 -RRB- denotes ]
T5909 28138-28140 , denotes ,
T5910 28140-28152 VBN denotes supplemented
T5911 28153-28157 IN denotes with
T5912 28158-28160 CD denotes 24
T5913 28161-28163 NN denotes mM
T5914 28164-28167 NN denotes KCl
T5915 28167-28168 -RRB- denotes )
T5916 28168-28169 . denotes .
T5917 28169-28382 sentence denotes Infection was performed the following day with defective retrovirus transducing the temperature-sensitive tsA58/U19 large T antigen and a selection neomycin-resistance cassette [27], as previously described [28].
T5918 28170-28179 NN denotes Infection
T5919 28184-28193 VBN denotes performed
T5920 28180-28183 VBD denotes was
T5921 28194-28197 DT denotes the
T5922 28208-28211 NN denotes day
T5923 28198-28207 JJ denotes following
T5924 28212-28216 IN denotes with
T5925 28217-28226 JJ denotes defective
T5926 28227-28237 NN denotes retrovirus
T5927 28238-28249 VBG denotes transducing
T5928 28250-28253 DT denotes the
T5929 28294-28301 NN denotes antigen
T5930 28254-28265 NN denotes temperature
T5931 28266-28275 JJ denotes sensitive
T5932 28265-28266 HYPH denotes -
T5933 28276-28281 NN denotes tsA58
T5934 28282-28285 NN denotes U19
T5935 28281-28282 HYPH denotes /
T5936 28286-28291 JJ denotes large
T5937 28292-28293 NN denotes T
T5938 28302-28305 CC denotes and
T5939 28306-28307 DT denotes a
T5940 28338-28346 NN denotes cassette
T5941 28308-28317 NN denotes selection
T5942 28318-28326 NN denotes neomycin
T5943 28327-28337 NN denotes resistance
T5944 28326-28327 HYPH denotes -
T5945 28347-28348 -LRB- denotes [
T5946 28348-28350 CD denotes 27
T5947 28350-28351 -RRB- denotes ]
T5948 28351-28353 , denotes ,
T5949 28353-28355 IN denotes as
T5950 28367-28376 VBN denotes described
T5951 28356-28366 RB denotes previously
T5952 28377-28378 -LRB- denotes [
T5953 28378-28380 CD denotes 28
T5954 28380-28381 -RRB- denotes ]
T5955 28381-28382 . denotes .
T5956 28382-28554 sentence denotes Following infection, cultures were shifted to the tsA58/U19 permissive growth temperature of 33°C and selection was in the same growth media as above, with 200 μg/ml G418.
T5957 28383-28392 VBG denotes Following
T5958 28418-28425 VBN denotes shifted
T5959 28393-28402 NN denotes infection
T5960 28402-28404 , denotes ,
T5961 28404-28412 NNS denotes cultures
T5962 28413-28417 VBD denotes were
T5963 28426-28428 IN denotes to
T5964 28429-28432 DT denotes the
T5965 28461-28472 NN denotes temperature
T5966 28433-28438 NN denotes tsA58
T5967 28439-28442 NN denotes U19
T5968 28438-28439 HYPH denotes /
T5969 28443-28453 JJ denotes permissive
T5970 28454-28460 NN denotes growth
T5971 28473-28475 IN denotes of
T5972 28476-28478 CD denotes 33
T5973 28478-28480 NN denotes °C
T5974 28481-28484 CC denotes and
T5975 28485-28494 NN denotes selection
T5976 28495-28498 VBD denotes was
T5977 28499-28501 IN denotes in
T5978 28502-28505 DT denotes the
T5979 28518-28523 NNS denotes media
T5980 28506-28510 JJ denotes same
T5981 28511-28517 NN denotes growth
T5982 28524-28526 IN denotes as
T5983 28527-28532 RB denotes above
T5984 28532-28534 , denotes ,
T5985 28534-28538 IN denotes with
T5986 28539-28542 CD denotes 200
T5987 28543-28545 NN denotes μg
T5988 28549-28553 NN denotes G418
T5989 28545-28546 SYM denotes /
T5990 28546-28548 NN denotes ml
T5991 28553-28554 . denotes .
T5992 28554-28693 sentence denotes Conditionally immortalized colonies were isolated 3–9 weeks post-infection and expanded for frozen stocks and further sub-clone isolation.
T5993 28555-28568 RB denotes Conditionally
T5994 28569-28581 VBN denotes immortalized
T5995 28582-28590 NNS denotes colonies
T5996 28596-28604 VBN denotes isolated
T5997 28591-28595 VBD denotes were
T5998 28605-28606 CD denotes 3
T5999 28607-28608 CD denotes 9
T6000 28606-28607 SYM denotes –
T6001 28609-28614 NNS denotes weeks
T6002 28615-28629 RB denotes post-infection
T6003 28630-28633 CC denotes and
T6004 28634-28642 VBN denotes expanded
T6005 28643-28646 IN denotes for
T6006 28647-28653 JJ denotes frozen
T6007 28654-28660 NNS denotes stocks
T6008 28661-28664 CC denotes and
T6009 28665-28672 JJ denotes further
T6010 28683-28692 NN denotes isolation
T6011 28673-28682 NN denotes sub-clone
T6012 28692-28693 . denotes .
T6013 28693-28826 sentence denotes Multiple clonal lines were obtained for each genotype and all phenotypes were confirmed in at least 2 independent CbCln3 cell lines.
T6014 28694-28702 JJ denotes Multiple
T6015 28710-28715 NNS denotes lines
T6016 28703-28709 JJ denotes clonal
T6017 28721-28729 VBN denotes obtained
T6018 28716-28720 VBD denotes were
T6019 28730-28733 IN denotes for
T6020 28734-28738 DT denotes each
T6021 28739-28747 NN denotes genotype
T6022 28748-28751 CC denotes and
T6023 28752-28755 DT denotes all
T6024 28756-28766 NNS denotes phenotypes
T6025 28767-28771 VBD denotes were
T6026 28772-28781 VBN denotes confirmed
T6027 28782-28784 IN denotes in
T6028 28785-28787 RB denotes at
T6029 28794-28795 CD denotes 2
T6030 28788-28793 RBS denotes least
T6031 28820-28825 NNS denotes lines
T6032 28796-28807 JJ denotes independent
T6033 28808-28814 NN denotes CbCln3
T6034 28815-28819 NN denotes cell
T6035 28825-28826 . denotes .
T6036 28826-28951 sentence denotes CbCln3 cell lines were maintained on 0.01% poly-ornithine coated dishes at 30–90% confluency, in 33°C and 5% CO2 atmosphere.
T6037 28827-28833 NN denotes CbCln3
T6038 28839-28844 NNS denotes lines
T6039 28834-28838 NN denotes cell
T6040 28850-28860 VBN denotes maintained
T6041 28845-28849 VBD denotes were
T6042 28861-28863 IN denotes on
T6043 28864-28868 CD denotes 0.01
T6044 28868-28869 NN denotes %
T6045 28892-28898 NNS denotes dishes
T6046 28870-28874 NN denotes poly
T6047 28875-28884 NN denotes ornithine
T6048 28874-28875 HYPH denotes -
T6049 28885-28891 VBN denotes coated
T6050 28899-28901 IN denotes at
T6051 28902-28904 CD denotes 30
T6052 28905-28907 CD denotes 90
T6053 28904-28905 SYM denotes –
T6054 28907-28908 NN denotes %
T6055 28909-28919 NN denotes confluency
T6056 28919-28921 , denotes ,
T6057 28921-28923 IN denotes in
T6058 28924-28926 CD denotes 33
T6059 28926-28928 NN denotes °C
T6060 28929-28932 CC denotes and
T6061 28933-28934 CD denotes 5
T6062 28940-28950 NN denotes atmosphere
T6063 28934-28935 SYM denotes %
T6064 28936-28939 NN denotes CO2
T6065 28950-28951 . denotes .
T6066 28951-29042 sentence denotes Passage number was recorded (up to ~20 passages), but had no apparent impact on phenotype.
T6067 28952-28959 NN denotes Passage
T6068 28960-28966 NN denotes number
T6069 28971-28979 VBN denotes recorded
T6070 28967-28970 VBD denotes was
T6071 28980-28981 -LRB- denotes (
T6072 28991-28999 NNS denotes passages
T6073 28981-28983 IN denotes up
T6074 28988-28990 CD denotes 20
T6075 28984-28986 IN denotes to
T6076 28987-28988 SYM denotes ~
T6077 28999-29000 -RRB- denotes )
T6078 29000-29002 , denotes ,
T6079 29002-29005 CC denotes but
T6080 29006-29009 VBD denotes had
T6081 29010-29012 DT denotes no
T6082 29022-29028 NN denotes impact
T6083 29013-29021 JJ denotes apparent
T6084 29029-29031 IN denotes on
T6085 29032-29041 NN denotes phenotype
T6086 29041-29042 . denotes .
T6087 29042-29210 sentence denotes Neuronal differentiation was as previously described [29] with the following cocktail: 10 ng/ml α-FGF, 250 μM IBMX, 200 nM TPA, 50 μM forskolin, 5 μM dopamine (Sigma).
T6088 29043-29051 JJ denotes Neuronal
T6089 29052-29067 NN denotes differentiation
T6090 29068-29071 VBD denotes was
T6091 29072-29074 IN denotes as
T6092 29086-29095 VBN denotes described
T6093 29075-29085 RB denotes previously
T6094 29096-29097 -LRB- denotes [
T6095 29097-29099 CD denotes 29
T6096 29099-29100 -RRB- denotes ]
T6097 29101-29105 IN denotes with
T6098 29106-29109 DT denotes the
T6099 29120-29128 NN denotes cocktail
T6100 29110-29119 JJ denotes following
T6101 29128-29130 : denotes :
T6102 29130-29132 CD denotes 10
T6103 29133-29135 NN denotes ng
T6104 29141-29144 NN denotes FGF
T6105 29135-29136 SYM denotes /
T6106 29136-29138 NN denotes ml
T6107 29139-29140 NN denotes α
T6108 29140-29141 HYPH denotes -
T6109 29144-29146 , denotes ,
T6110 29146-29149 CD denotes 250
T6111 29150-29152 NN denotes μM
T6112 29153-29157 NN denotes IBMX
T6113 29157-29159 , denotes ,
T6114 29159-29162 CD denotes 200
T6115 29163-29165 NN denotes nM
T6116 29166-29169 NN denotes TPA
T6117 29169-29171 , denotes ,
T6118 29171-29173 CD denotes 50
T6119 29174-29176 NN denotes μM
T6120 29177-29186 NN denotes forskolin
T6121 29186-29188 , denotes ,
T6122 29188-29189 CD denotes 5
T6123 29190-29192 NN denotes μM
T6124 29193-29201 NN denotes dopamine
T6125 29202-29203 -LRB- denotes (
T6126 29203-29208 NNP denotes Sigma
T6127 29208-29209 -RRB- denotes )
T6128 29209-29210 . denotes .
T6176 29265-29269 NN denotes tail
T6165 29212-29222 NN denotes Genotyping
T6166 29223-29226 CC denotes and
T6167 29227-29229 NN denotes RT
T6168 29230-29233 NN denotes PCR
T6169 29229-29230 HYPH denotes -
T6170 29233-29331 sentence denotes Genomic DNA was extracted from tail biopsies and cell pellets as described (Cotman et al., 2002).
T6171 29234-29241 JJ denotes Genomic
T6172 29242-29245 NN denotes DNA
T6173 29250-29259 VBN denotes extracted
T6174 29246-29249 VBD denotes was
T6175 29260-29264 IN denotes from
T6177 29270-29278 NNS denotes biopsies
T6178 29279-29282 CC denotes and
T6179 29283-29287 NN denotes cell
T6180 29288-29295 NNS denotes pellets
T6181 29296-29298 IN denotes as
T6182 29299-29308 VBN denotes described
T6183 29309-29310 -LRB- denotes (
T6184 29310-29316 NNP denotes Cotman
T6185 29317-29319 FW denotes et
T6186 29320-29323 FW denotes al.
T6187 29323-29325 , denotes ,
T6188 29325-29329 CD denotes 2002
T6189 29329-29330 -RRB- denotes )
T6190 29330-29331 . denotes .
T6191 29331-29620 sentence denotes Cln3Δex7/8 knock-in allele PCR genotyping was with wild-type primers, WtF (5'-CAGCATCTCCTCAGGGCTA-3') and WtR (5'-CCAACATAGAAAGTAGGGTGTGC-3') to yield a ~250 bp band and knock-in primers, 552F (5'-GAGCTTTGTTCTGGTTGCCTTC-3') and Ex9RA (5'-GCAGTCTCTGCCTCGTTTTCT-3') to yield a ~500 bp band.
T6192 29332-29340 NN denotes Cln3Δex7
T6193 29352-29358 NN denotes allele
T6194 29340-29341 HYPH denotes /
T6195 29341-29342 CD denotes 8
T6196 29343-29348 VB denotes knock
T6197 29348-29349 HYPH denotes -
T6198 29349-29351 RP denotes in
T6199 29363-29373 NN denotes genotyping
T6200 29359-29362 NN denotes PCR
T6201 29374-29377 VBD denotes was
T6202 29378-29382 IN denotes with
T6203 29383-29387 JJ denotes wild
T6204 29388-29392 NN denotes type
T6205 29387-29388 HYPH denotes -
T6206 29393-29400 NNS denotes primers
T6207 29400-29402 , denotes ,
T6208 29402-29405 NN denotes WtF
T6209 29406-29407 -LRB- denotes (
T6210 29407-29408 CD denotes 5
T6211 29410-29429 NN denotes CAGCATCTCCTCAGGGCTA
T6212 29408-29409 SYM denotes '
T6213 29409-29410 HYPH denotes -
T6214 29429-29430 HYPH denotes -
T6215 29430-29431 CD denotes 3
T6216 29431-29432 SYM denotes '
T6217 29432-29433 -RRB- denotes )
T6218 29434-29437 CC denotes and
T6219 29438-29441 NN denotes WtR
T6220 29442-29443 -LRB- denotes (
T6221 29443-29444 CD denotes 5
T6222 29446-29469 NN denotes CCAACATAGAAAGTAGGGTGTGC
T6223 29444-29445 SYM denotes '
T6224 29445-29446 HYPH denotes -
T6225 29469-29470 HYPH denotes -
T6226 29470-29471 CD denotes 3
T6227 29471-29472 SYM denotes '
T6228 29472-29473 -RRB- denotes )
T6229 29474-29476 TO denotes to
T6230 29477-29482 VB denotes yield
T6231 29483-29484 DT denotes a
T6232 29493-29497 NN denotes band
T6233 29485-29486 SYM denotes ~
T6234 29486-29489 CD denotes 250
T6235 29490-29492 NN denotes bp
T6236 29498-29501 CC denotes and
T6237 29502-29507 VB denotes knock
T6238 29511-29518 NNS denotes primers
T6239 29507-29508 HYPH denotes -
T6240 29508-29510 RP denotes in
T6241 29518-29520 , denotes ,
T6242 29520-29524 NN denotes 552F
T6243 29525-29526 -LRB- denotes (
T6244 29526-29527 CD denotes 5
T6245 29529-29551 NN denotes GAGCTTTGTTCTGGTTGCCTTC
T6246 29527-29528 SYM denotes '
T6247 29528-29529 HYPH denotes -
T6248 29551-29552 HYPH denotes -
T6249 29552-29553 CD denotes 3
T6250 29553-29554 SYM denotes '
T6251 29554-29555 -RRB- denotes )
T6252 29556-29559 CC denotes and
T6253 29560-29565 NN denotes Ex9RA
T6254 29566-29567 -LRB- denotes (
T6255 29567-29568 CD denotes 5
T6256 29570-29591 NN denotes GCAGTCTCTGCCTCGTTTTCT
T6257 29568-29569 SYM denotes '
T6258 29569-29570 HYPH denotes -
T6259 29591-29592 HYPH denotes -
T6260 29592-29593 CD denotes 3
T6261 29593-29594 SYM denotes '
T6262 29594-29595 -RRB- denotes )
T6263 29596-29598 TO denotes to
T6264 29599-29604 VB denotes yield
T6265 29605-29606 DT denotes a
T6266 29615-29619 NN denotes band
T6267 29607-29608 SYM denotes ~
T6268 29608-29611 CD denotes 500
T6269 29612-29614 NN denotes bp
T6270 29619-29620 . denotes .
T6271 29620-29739 sentence denotes PCR cycling conditions were 95°C for 30 seconds, 58°C for 30 seconds, and 72°C for 35 seconds, repeated for 34 cycles.
T6272 29621-29624 NN denotes PCR
T6273 29625-29632 NN denotes cycling
T6274 29633-29643 NNS denotes conditions
T6275 29644-29648 VBD denotes were
T6276 29649-29651 CD denotes 95
T6277 29651-29653 NN denotes °C
T6278 29654-29657 IN denotes for
T6279 29658-29660 CD denotes 30
T6280 29661-29668 NNS denotes seconds
T6281 29668-29670 , denotes ,
T6282 29670-29672 CD denotes 58
T6283 29672-29674 NN denotes °C
T6284 29675-29678 IN denotes for
T6285 29679-29681 CD denotes 30
T6286 29682-29689 NNS denotes seconds
T6287 29689-29691 , denotes ,
T6288 29691-29694 CC denotes and
T6289 29695-29697 CD denotes 72
T6290 29697-29699 NN denotes °C
T6291 29700-29703 IN denotes for
T6292 29704-29706 CD denotes 35
T6293 29707-29714 NNS denotes seconds
T6294 29714-29716 , denotes ,
T6295 29716-29724 VBN denotes repeated
T6296 29725-29728 IN denotes for
T6297 29729-29731 CD denotes 34
T6298 29732-29738 NNS denotes cycles
T6299 29738-29739 . denotes .
T6300 29739-29835 sentence denotes Total RNA isolation and Cln3 RT-PCR primers and procedures have been previously described [12].
T6301 29740-29745 JJ denotes Total
T6302 29750-29759 NN denotes isolation
T6303 29746-29749 NN denotes RNA
T6304 29820-29829 VBN denotes described
T6305 29760-29763 CC denotes and
T6306 29764-29768 NN denotes Cln3
T6307 29776-29783 NNS denotes primers
T6308 29769-29771 NN denotes RT
T6309 29772-29775 NN denotes PCR
T6310 29771-29772 HYPH denotes -
T6311 29784-29787 CC denotes and
T6312 29788-29798 NNS denotes procedures
T6313 29799-29803 VBP denotes have
T6314 29804-29808 VBN denotes been
T6315 29809-29819 RB denotes previously
T6316 29830-29831 -LRB- denotes [
T6317 29831-29833 CD denotes 12
T6318 29833-29834 -RRB- denotes ]
T6319 29834-29835 . denotes .
T6369 29837-29844 NN denotes Subunit
T6370 29845-29846 NN denotes c
T6371 29847-29859 NN denotes accumulation
T6372 29860-29865 NN denotes assay
T6373 29865-30076 sentence denotes Cells were seeded into 4-well chamber-slides (Falcon) at a density of 5 × 104 cells per well for microscopy studies, or into 100 mm dishes (Falcon) at a density of 5 × 105 cells per dish for protein extraction.
T6374 29866-29871 NNS denotes Cells
T6375 29877-29883 VBN denotes seeded
T6376 29872-29876 VBD denotes were
T6377 29884-29888 IN denotes into
T6378 29889-29890 CD denotes 4
T6379 29891-29895 NN denotes well
T6380 29890-29891 HYPH denotes -
T6381 29904-29910 NNS denotes slides
T6382 29896-29903 NN denotes chamber
T6383 29903-29904 HYPH denotes -
T6384 29911-29912 -LRB- denotes (
T6385 29912-29918 NNP denotes Falcon
T6386 29918-29919 -RRB- denotes )
T6387 29920-29922 IN denotes at
T6388 29923-29924 DT denotes a
T6389 29925-29932 NN denotes density
T6390 29933-29935 IN denotes of
T6391 29936-29937 CD denotes 5
T6392 29940-29943 CD denotes 104
T6393 29938-29939 SYM denotes ×
T6394 29944-29949 NNS denotes cells
T6395 29950-29953 IN denotes per
T6396 29954-29958 NN denotes well
T6397 29959-29962 IN denotes for
T6398 29963-29973 NN denotes microscopy
T6399 29974-29981 NNS denotes studies
T6400 29981-29983 , denotes ,
T6401 29983-29985 CC denotes or
T6402 29986-29990 IN denotes into
T6403 29991-29994 CD denotes 100
T6404 29995-29997 NN denotes mm
T6405 29998-30004 NNS denotes dishes
T6406 30005-30006 -LRB- denotes (
T6407 30006-30012 NNP denotes Falcon
T6408 30012-30013 -RRB- denotes )
T6409 30014-30016 IN denotes at
T6410 30017-30018 DT denotes a
T6411 30019-30026 NN denotes density
T6412 30027-30029 IN denotes of
T6413 30030-30031 CD denotes 5
T6414 30034-30037 CD denotes 105
T6415 30032-30033 SYM denotes ×
T6416 30038-30043 NNS denotes cells
T6417 30044-30047 IN denotes per
T6418 30048-30052 NN denotes dish
T6419 30053-30056 IN denotes for
T6420 30057-30064 NN denotes protein
T6421 30065-30075 NN denotes extraction
T6422 30075-30076 . denotes .
T6423 30076-30194 sentence denotes Cells were typically >95% confluent one day post-plating, and the following day was considered 1-day post-confluency.
T6424 30077-30082 NNS denotes Cells
T6425 30083-30087 VBD denotes were
T6426 30088-30097 RB denotes typically
T6427 30098-30099 SYM denotes >
T6428 30099-30101 CD denotes 95
T6429 30101-30102 NN denotes %
T6430 30103-30112 JJ denotes confluent
T6431 30113-30116 CD denotes one
T6432 30117-30120 NN denotes day
T6433 30121-30133 RB denotes post-plating
T6434 30133-30135 , denotes ,
T6435 30135-30138 CC denotes and
T6436 30139-30142 DT denotes the
T6437 30153-30156 NN denotes day
T6438 30143-30152 JJ denotes following
T6439 30161-30171 VBN denotes considered
T6440 30157-30160 VBD denotes was
T6441 30172-30173 CD denotes 1
T6442 30174-30177 NN denotes day
T6443 30173-30174 HYPH denotes -
T6444 30178-30193 RB denotes post-confluency
T6445 30193-30194 . denotes .
T6446 30194-30444 sentence denotes At the indicated times, cells were either fixed with 4% formaldehyde in phosphate buffered saline (PBS), pH 7.4, for 20 minutes and processed for autofluorescence/subunit c immunostaining, or cell pellets were collected for total protein extraction.
T6447 30195-30197 IN denotes At
T6448 30237-30242 VBN denotes fixed
T6449 30198-30201 DT denotes the
T6450 30212-30217 NNS denotes times
T6451 30202-30211 VBN denotes indicated
T6452 30217-30219 , denotes ,
T6453 30219-30224 NNS denotes cells
T6454 30225-30229 VBD denotes were
T6455 30230-30236 RB denotes either
T6456 30243-30247 IN denotes with
T6457 30248-30249 CD denotes 4
T6458 30249-30250 NN denotes %
T6459 30251-30263 NN denotes formaldehyde
T6460 30264-30266 IN denotes in
T6461 30267-30276 NN denotes phosphate
T6462 30286-30292 NN denotes saline
T6463 30277-30285 VBN denotes buffered
T6464 30293-30294 -LRB- denotes (
T6465 30294-30297 NN denotes PBS
T6466 30297-30298 -RRB- denotes )
T6467 30298-30300 , denotes ,
T6468 30300-30302 NN denotes pH
T6469 30303-30306 CD denotes 7.4
T6470 30306-30308 , denotes ,
T6471 30308-30311 IN denotes for
T6472 30312-30314 CD denotes 20
T6473 30315-30322 NNS denotes minutes
T6474 30323-30326 CC denotes and
T6475 30327-30336 VBN denotes processed
T6476 30337-30340 IN denotes for
T6477 30341-30357 NN denotes autofluorescence
T6478 30357-30358 HYPH denotes /
T6479 30358-30365 NN denotes subunit
T6480 30366-30367 NN denotes c
T6481 30368-30382 NN denotes immunostaining
T6482 30382-30384 , denotes ,
T6483 30384-30386 CC denotes or
T6484 30387-30391 NN denotes cell
T6485 30392-30399 NNS denotes pellets
T6486 30405-30414 VBN denotes collected
T6487 30400-30404 VBD denotes were
T6488 30415-30418 IN denotes for
T6489 30419-30424 JJ denotes total
T6490 30433-30443 NN denotes extraction
T6491 30425-30432 NN denotes protein
T6492 30443-30444 . denotes .
T6493 30444-30645 sentence denotes Alternatively, cells were fixed with 2.5% glutaraldehyde/2% paraformaldehyde in 0.1 M cacodylate buffer, pH 7.4 for 1 hour and subsequently post-fixed and processed for TEM analysis as described [12].
T6494 30445-30458 RB denotes Alternatively
T6495 30471-30476 VBN denotes fixed
T6496 30458-30460 , denotes ,
T6497 30460-30465 NNS denotes cells
T6498 30466-30470 VBD denotes were
T6499 30477-30481 IN denotes with
T6500 30482-30485 CD denotes 2.5
T6501 30485-30486 NN denotes %
T6502 30487-30501 NN denotes glutaraldehyde
T6503 30501-30502 HYPH denotes /
T6504 30502-30503 CD denotes 2
T6505 30503-30504 NN denotes %
T6506 30505-30521 NN denotes paraformaldehyde
T6507 30522-30524 IN denotes in
T6508 30525-30528 CD denotes 0.1
T6509 30529-30530 NN denotes M
T6510 30542-30548 NN denotes buffer
T6511 30531-30541 NN denotes cacodylate
T6512 30548-30550 , denotes ,
T6513 30550-30552 NN denotes pH
T6514 30553-30556 CD denotes 7.4
T6515 30557-30560 IN denotes for
T6516 30561-30562 CD denotes 1
T6517 30563-30567 NN denotes hour
T6518 30568-30571 CC denotes and
T6519 30572-30584 RB denotes subsequently
T6520 30585-30595 VBN denotes post-fixed
T6521 30596-30599 CC denotes and
T6522 30600-30609 VBN denotes processed
T6523 30610-30613 IN denotes for
T6524 30614-30617 NN denotes TEM
T6525 30618-30626 NN denotes analysis
T6526 30627-30629 IN denotes as
T6527 30630-30639 VBN denotes described
T6528 30640-30641 -LRB- denotes [
T6529 30641-30643 CD denotes 12
T6530 30643-30644 -RRB- denotes ]
T6531 30644-30645 . denotes .
T6532 30645-30740 sentence denotes In confocal microscopy studies, autofluorescent signal was observed over multiple wavelengths.
T6533 30646-30648 IN denotes In
T6534 30705-30713 VBN denotes observed
T6535 30649-30657 JJ denotes confocal
T6536 30669-30676 NNS denotes studies
T6537 30658-30668 NN denotes microscopy
T6538 30676-30678 , denotes ,
T6539 30678-30693 JJ denotes autofluorescent
T6540 30694-30700 NN denotes signal
T6541 30701-30704 VBD denotes was
T6542 30714-30718 IN denotes over
T6543 30719-30727 JJ denotes multiple
T6544 30728-30739 NNS denotes wavelengths
T6545 30739-30740 . denotes .
T6546 30740-30857 sentence denotes For co-staining, settings were reduced such that autofluorescent signal did not contribute to antibody label signal.
T6547 30741-30744 IN denotes For
T6548 30772-30779 VBN denotes reduced
T6549 30745-30756 NN denotes co-staining
T6550 30756-30758 , denotes ,
T6551 30758-30766 NNS denotes settings
T6552 30767-30771 VBD denotes were
T6553 30780-30784 JJ denotes such
T6554 30785-30789 IN denotes that
T6555 30821-30831 VB denotes contribute
T6556 30790-30805 JJ denotes autofluorescent
T6557 30806-30812 NN denotes signal
T6558 30813-30816 VBD denotes did
T6559 30817-30820 RB denotes not
T6560 30832-30834 IN denotes to
T6561 30835-30843 NN denotes antibody
T6562 30850-30856 NN denotes signal
T6563 30844-30849 NN denotes label
T6564 30856-30857 . denotes .
T6658 30859-30873 NN denotes Immunostaining
T6659 30889-30897 NN denotes analysis
T6660 30874-30877 CC denotes and
T6661 30878-30888 NN denotes Immunoblot
T6662 30897-31058 sentence denotes For immunostaining, cells were seeded at a density of 3–5 × 104 cells per well in 4-well chamber-slides and grown overnight at 33°C, unless indicated otherwise.
T6663 30898-30901 IN denotes For
T6664 30929-30935 VBN denotes seeded
T6665 30902-30916 NN denotes immunostaining
T6666 30916-30918 , denotes ,
T6667 30918-30923 NNS denotes cells
T6668 30924-30928 VBD denotes were
T6669 30936-30938 IN denotes at
T6670 30939-30940 DT denotes a
T6671 30941-30948 NN denotes density
T6672 30949-30951 IN denotes of
T6673 30952-30953 CD denotes 3
T6674 30954-30955 CD denotes 5
T6675 30953-30954 SYM denotes –
T6676 30958-30961 CD denotes 104
T6677 30956-30957 SYM denotes ×
T6678 30962-30967 NNS denotes cells
T6679 30968-30971 IN denotes per
T6680 30972-30976 NN denotes well
T6681 30977-30979 IN denotes in
T6682 30980-30981 CD denotes 4
T6683 30982-30986 NN denotes well
T6684 30981-30982 HYPH denotes -
T6685 30995-31001 NNS denotes slides
T6686 30987-30994 NN denotes chamber
T6687 30994-30995 HYPH denotes -
T6688 31002-31005 CC denotes and
T6689 31006-31011 VBN denotes grown
T6690 31012-31021 RB denotes overnight
T6691 31022-31024 IN denotes at
T6692 31025-31027 CD denotes 33
T6693 31027-31029 NN denotes °C
T6694 31029-31031 , denotes ,
T6695 31031-31037 IN denotes unless
T6696 31038-31047 VBN denotes indicated
T6697 31048-31057 RB denotes otherwise
T6698 31057-31058 . denotes .
T6699 31058-31242 sentence denotes Fixation was with ice-cold 4% formaldehyde in PBS, pH 7.4, for 20 minutes, or with ice-cold methanol/acetone (1:1) for 10 minutes at -20°C followed by air-drying (antibody-dependent).
T6700 31059-31067 NN denotes Fixation
T6701 31068-31071 VBD denotes was
T6702 31072-31076 IN denotes with
T6703 31077-31080 NN denotes ice
T6704 31081-31085 JJ denotes cold
T6705 31080-31081 HYPH denotes -
T6706 31089-31101 NN denotes formaldehyde
T6707 31086-31087 CD denotes 4
T6708 31087-31088 NN denotes %
T6709 31102-31104 IN denotes in
T6710 31105-31108 NN denotes PBS
T6711 31108-31110 , denotes ,
T6712 31110-31112 NN denotes pH
T6713 31113-31116 CD denotes 7.4
T6714 31116-31118 , denotes ,
T6715 31118-31121 IN denotes for
T6716 31122-31124 CD denotes 20
T6717 31125-31132 NNS denotes minutes
T6718 31132-31134 , denotes ,
T6719 31134-31136 CC denotes or
T6720 31137-31141 IN denotes with
T6721 31142-31145 NN denotes ice
T6722 31146-31150 JJ denotes cold
T6723 31145-31146 HYPH denotes -
T6724 31160-31167 NN denotes acetone
T6725 31151-31159 NN denotes methanol
T6726 31159-31160 HYPH denotes /
T6727 31168-31169 -LRB- denotes (
T6728 31169-31170 CD denotes 1
T6729 31170-31171 SYM denotes :
T6730 31171-31172 CD denotes 1
T6731 31172-31173 -RRB- denotes )
T6732 31174-31177 IN denotes for
T6733 31178-31180 CD denotes 10
T6734 31181-31188 NNS denotes minutes
T6735 31189-31191 IN denotes at
T6736 31192-31193 SYM denotes -
T6737 31193-31195 CD denotes 20
T6738 31195-31197 NN denotes °C
T6739 31198-31206 VBN denotes followed
T6740 31207-31209 IN denotes by
T6741 31210-31213 NN denotes air
T6742 31214-31220 NN denotes drying
T6743 31213-31214 HYPH denotes -
T6744 31221-31222 -LRB- denotes (
T6745 31231-31240 JJ denotes dependent
T6746 31222-31230 NN denotes antibody
T6747 31230-31231 HYPH denotes -
T6748 31240-31241 -RRB- denotes )
T6749 31241-31242 . denotes .
T6750 31242-31661 sentence denotes Cells were washed with PBS at least 2 times, 5 minutes per wash, between each of the following steps of the staining procedure: 0.1 M glycine in PBS for 5 minutes, 0.05% or 0.1% (antibody-dependent) Triton X-100 (Fisher Scientific) in PBS for 5 minutes, 2% bovine serum albumin (BSA) in PBS for 30 minutes, primary antibody diluted in 2% BSA/PBS for 90 minutes, secondary antibody diluted in 2% BSA/PBS for 60 minutes.
T6751 31243-31248 NNS denotes Cells
T6752 31254-31260 VBN denotes washed
T6753 31249-31253 VBD denotes were
T6754 31261-31265 IN denotes with
T6755 31266-31269 NN denotes PBS
T6756 31270-31272 RB denotes at
T6757 31279-31280 CD denotes 2
T6758 31273-31278 RBS denotes least
T6759 31281-31286 NNS denotes times
T6760 31286-31288 , denotes ,
T6761 31288-31289 CD denotes 5
T6762 31290-31297 NNS denotes minutes
T6763 31298-31301 IN denotes per
T6764 31302-31306 NN denotes wash
T6765 31306-31308 , denotes ,
T6766 31308-31315 IN denotes between
T6767 31316-31320 DT denotes each
T6768 31321-31323 IN denotes of
T6769 31324-31327 DT denotes the
T6770 31338-31343 NNS denotes steps
T6771 31328-31337 JJ denotes following
T6772 31344-31346 IN denotes of
T6773 31347-31350 DT denotes the
T6774 31360-31369 NN denotes procedure
T6775 31351-31359 NN denotes staining
T6776 31369-31371 : denotes :
T6777 31371-31374 CD denotes 0.1
T6778 31375-31376 NN denotes M
T6779 31377-31384 NN denotes glycine
T6780 31385-31387 IN denotes in
T6781 31388-31391 NN denotes PBS
T6782 31392-31395 IN denotes for
T6783 31396-31397 CD denotes 5
T6784 31398-31405 NNS denotes minutes
T6785 31405-31407 , denotes ,
T6786 31407-31411 CD denotes 0.05
T6787 31411-31412 NN denotes %
T6788 31449-31450 NN denotes X
T6789 31413-31415 CC denotes or
T6790 31416-31419 CD denotes 0.1
T6791 31419-31420 NN denotes %
T6792 31421-31422 -LRB- denotes (
T6793 31422-31430 NN denotes antibody
T6794 31431-31440 JJ denotes dependent
T6795 31430-31431 HYPH denotes -
T6796 31440-31441 -RRB- denotes )
T6797 31442-31448 NN denotes Triton
T6798 31450-31451 HYPH denotes -
T6799 31451-31454 CD denotes 100
T6800 31455-31456 -LRB- denotes (
T6801 31463-31473 NNP denotes Scientific
T6802 31456-31462 NNP denotes Fisher
T6803 31473-31474 -RRB- denotes )
T6804 31475-31477 IN denotes in
T6805 31478-31481 NN denotes PBS
T6806 31482-31485 IN denotes for
T6807 31486-31487 CD denotes 5
T6808 31488-31495 NNS denotes minutes
T6809 31495-31497 , denotes ,
T6810 31497-31498 CD denotes 2
T6811 31498-31499 NN denotes %
T6812 31500-31506 JJ denotes bovine
T6813 31513-31520 NN denotes albumin
T6814 31507-31512 NN denotes serum
T6815 31521-31522 -LRB- denotes (
T6816 31522-31525 NN denotes BSA
T6817 31525-31526 -RRB- denotes )
T6818 31527-31529 IN denotes in
T6819 31530-31533 NN denotes PBS
T6820 31534-31537 IN denotes for
T6821 31538-31540 CD denotes 30
T6822 31541-31548 NNS denotes minutes
T6823 31548-31550 , denotes ,
T6824 31550-31557 JJ denotes primary
T6825 31558-31566 NN denotes antibody
T6826 31567-31574 VBN denotes diluted
T6827 31575-31577 IN denotes in
T6828 31578-31579 CD denotes 2
T6829 31579-31580 NN denotes %
T6830 31585-31588 NN denotes PBS
T6831 31581-31584 NN denotes BSA
T6832 31584-31585 HYPH denotes /
T6833 31589-31592 IN denotes for
T6834 31593-31595 CD denotes 90
T6835 31596-31603 NNS denotes minutes
T6836 31603-31605 , denotes ,
T6837 31605-31614 JJ denotes secondary
T6838 31615-31623 NN denotes antibody
T6839 31624-31631 VBN denotes diluted
T6840 31632-31634 IN denotes in
T6841 31635-31636 CD denotes 2
T6842 31636-31637 NN denotes %
T6843 31642-31645 NN denotes PBS
T6844 31638-31641 NN denotes BSA
T6845 31641-31642 HYPH denotes /
T6846 31646-31649 IN denotes for
T6847 31650-31652 CD denotes 60
T6848 31653-31660 NNS denotes minutes
T6849 31660-31661 . denotes .
T6850 31661-31715 sentence denotes All incubations were carried out at room temperature.
T6851 31662-31665 DT denotes All
T6852 31666-31677 NNS denotes incubations
T6853 31683-31690 VBN denotes carried
T6854 31678-31682 VBD denotes were
T6855 31691-31694 RP denotes out
T6856 31695-31697 IN denotes at
T6857 31698-31702 NN denotes room
T6858 31703-31714 NN denotes temperature
T6859 31714-31715 . denotes .
T6860 31715-31966 sentence denotes Following staining procedures, slides were coverslipped with Vectashield mounting medium (Vector Laboratories) and analyzed on a BioRad Radiance 2100 confocal microscope (Biorad), with identical exposure settings for wild-type and mutant like images.
T6861 31716-31725 VBG denotes Following
T6862 31759-31771 VBN denotes coverslipped
T6863 31726-31734 NN denotes staining
T6864 31735-31745 NNS denotes procedures
T6865 31745-31747 , denotes ,
T6866 31747-31753 NNS denotes slides
T6867 31754-31758 VBD denotes were
T6868 31772-31776 IN denotes with
T6869 31777-31788 NNP denotes Vectashield
T6870 31798-31804 NN denotes medium
T6871 31789-31797 NN denotes mounting
T6872 31805-31806 -LRB- denotes (
T6873 31813-31825 NNP denotes Laboratories
T6874 31806-31812 NNP denotes Vector
T6875 31825-31826 -RRB- denotes )
T6876 31827-31830 CC denotes and
T6877 31831-31839 VBN denotes analyzed
T6878 31840-31842 IN denotes on
T6879 31843-31844 DT denotes a
T6880 31875-31885 NN denotes microscope
T6881 31845-31851 NNP denotes BioRad
T6882 31852-31860 NNP denotes Radiance
T6883 31861-31865 CD denotes 2100
T6884 31866-31874 JJ denotes confocal
T6885 31886-31887 -LRB- denotes (
T6886 31887-31893 NNP denotes Biorad
T6887 31893-31894 -RRB- denotes )
T6888 31894-31896 , denotes ,
T6889 31896-31900 IN denotes with
T6890 31901-31910 JJ denotes identical
T6891 31920-31928 NNS denotes settings
T6892 31911-31919 NN denotes exposure
T6893 31929-31932 IN denotes for
T6894 31933-31937 JJ denotes wild
T6895 31938-31942 NN denotes type
T6896 31937-31938 HYPH denotes -
T6897 31959-31965 NNS denotes images
T6898 31943-31946 CC denotes and
T6899 31947-31953 JJ denotes mutant
T6900 31954-31958 JJ denotes like
T6901 31965-31966 . denotes .
T6902 31966-32101 sentence denotes All comparisons of wild-type and mutant staining were performed in Adobe Photoshop with identical brightness and contrast adjustments.
T6903 31967-31970 DT denotes All
T6904 31971-31982 NNS denotes comparisons
T6905 32021-32030 VBN denotes performed
T6906 31983-31985 IN denotes of
T6907 31986-31990 JJ denotes wild
T6908 31991-31995 NN denotes type
T6909 31990-31991 HYPH denotes -
T6910 32007-32015 NN denotes staining
T6911 31996-31999 CC denotes and
T6912 32000-32006 NN denotes mutant
T6913 32016-32020 VBD denotes were
T6914 32031-32033 IN denotes in
T6915 32034-32039 NNP denotes Adobe
T6916 32040-32049 NNP denotes Photoshop
T6917 32050-32054 IN denotes with
T6918 32055-32064 JJ denotes identical
T6919 32089-32100 NNS denotes adjustments
T6920 32065-32075 NN denotes brightness
T6921 32076-32079 CC denotes and
T6922 32080-32088 NN denotes contrast
T6923 32100-32101 . denotes .
T6924 32101-32364 sentence denotes Total proteins were isolated from cell pellets by extraction with ice-cold 20 mM Tris, pH 7.4, 1% Triton X-100 (membrane-research grade, Roche), plus protease inhibitors (Complete mini tablet, 0.7 μg/ml pepstatin A, 2 μg/ml aprotinin, 5 μg/ml leupeptin [Roche]).
T6925 32102-32107 JJ denotes Total
T6926 32108-32116 NN denotes proteins
T6927 32122-32130 VBN denotes isolated
T6928 32117-32121 VBD denotes were
T6929 32131-32135 IN denotes from
T6930 32136-32140 NN denotes cell
T6931 32141-32148 NNS denotes pellets
T6932 32149-32151 IN denotes by
T6933 32152-32162 NN denotes extraction
T6934 32163-32167 IN denotes with
T6935 32168-32171 NN denotes ice
T6936 32183-32187 NN denotes Tris
T6937 32171-32172 HYPH denotes -
T6938 32172-32176 JJ denotes cold
T6939 32177-32179 CD denotes 20
T6940 32180-32182 NN denotes mM
T6941 32187-32189 , denotes ,
T6942 32189-32191 NN denotes pH
T6943 32192-32195 CD denotes 7.4
T6944 32195-32197 , denotes ,
T6945 32197-32198 CD denotes 1
T6946 32198-32199 NN denotes %
T6947 32207-32208 NN denotes X
T6948 32200-32206 NN denotes Triton
T6949 32208-32209 HYPH denotes -
T6950 32209-32212 CD denotes 100
T6951 32213-32214 -LRB- denotes (
T6952 32239-32244 NNP denotes Roche
T6953 32214-32222 NN denotes membrane
T6954 32223-32231 NN denotes research
T6955 32222-32223 HYPH denotes -
T6956 32232-32237 NN denotes grade
T6957 32237-32239 , denotes ,
T6958 32244-32245 -RRB- denotes )
T6959 32245-32247 , denotes ,
T6960 32247-32251 CC denotes plus
T6961 32252-32260 NN denotes protease
T6962 32261-32271 NNS denotes inhibitors
T6963 32272-32273 -LRB- denotes (
T6964 32273-32281 JJ denotes Complete
T6965 32287-32293 NN denotes tablet
T6966 32282-32286 NN denotes mini
T6967 32293-32295 , denotes ,
T6968 32295-32298 CD denotes 0.7
T6969 32299-32301 NN denotes μg
T6970 32315-32316 NN denotes A
T6971 32301-32302 SYM denotes /
T6972 32302-32304 NN denotes ml
T6973 32305-32314 NN denotes pepstatin
T6974 32316-32318 , denotes ,
T6975 32318-32319 CD denotes 2
T6976 32320-32322 NN denotes μg
T6977 32326-32335 NN denotes aprotinin
T6978 32322-32323 SYM denotes /
T6979 32323-32325 NN denotes ml
T6980 32335-32337 , denotes ,
T6981 32337-32338 CD denotes 5
T6982 32339-32341 NN denotes μg
T6983 32345-32354 NN denotes leupeptin
T6984 32341-32342 SYM denotes /
T6985 32342-32344 NN denotes ml
T6986 32355-32356 -LRB- denotes [
T6987 32356-32361 NNP denotes Roche
T6988 32361-32362 -RRB- denotes ]
T6989 32362-32363 -RRB- denotes )
T6990 32363-32364 . denotes .
T6991 32364-32509 sentence denotes Following homogenization through a 25-gauge needle (~10 passes), extracts were centrifuged at 1000 × g for 10 minutes, at 4°C, to remove debris.
T6992 32365-32374 VBG denotes Following
T6993 32444-32455 VBN denotes centrifuged
T6994 32375-32389 NN denotes homogenization
T6995 32390-32397 IN denotes through
T6996 32398-32399 DT denotes a
T6997 32409-32415 NN denotes needle
T6998 32400-32402 CD denotes 25
T6999 32403-32408 NN denotes gauge
T7000 32402-32403 HYPH denotes -
T7001 32416-32417 -LRB- denotes (
T7002 32421-32427 NNS denotes passes
T7003 32417-32418 SYM denotes ~
T7004 32418-32420 CD denotes 10
T7005 32427-32428 -RRB- denotes )
T7006 32428-32430 , denotes ,
T7007 32430-32438 NNS denotes extracts
T7008 32439-32443 VBD denotes were
T7009 32456-32458 IN denotes at
T7010 32459-32463 CD denotes 1000
T7011 32466-32467 NN denotes g
T7012 32464-32465 SYM denotes ×
T7013 32468-32471 IN denotes for
T7014 32472-32474 CD denotes 10
T7015 32475-32482 NNS denotes minutes
T7016 32482-32484 , denotes ,
T7017 32484-32486 IN denotes at
T7018 32487-32488 CD denotes 4
T7019 32488-32490 NN denotes °C
T7020 32490-32492 , denotes ,
T7021 32492-32494 TO denotes to
T7022 32495-32501 VB denotes remove
T7023 32502-32508 NN denotes debris
T7024 32508-32509 . denotes .
T7025 32509-32658 sentence denotes Typically, 20–40 μg of protein (determined by Bio-rad Dc Protein Assay) was separated by SDS-PAGE, for subsequent immunoblotting, as described [12].
T7026 32510-32519 RB denotes Typically
T7027 32586-32595 VBN denotes separated
T7028 32519-32521 , denotes ,
T7029 32521-32523 CD denotes 20
T7030 32524-32526 CD denotes 40
T7031 32523-32524 SYM denotes –
T7032 32527-32529 NN denotes μg
T7033 32530-32532 IN denotes of
T7034 32533-32540 NN denotes protein
T7035 32541-32542 -LRB- denotes (
T7036 32542-32552 VBN denotes determined
T7037 32553-32555 IN denotes by
T7038 32556-32559 NNP denotes Bio
T7039 32560-32563 NNP denotes rad
T7040 32559-32560 HYPH denotes -
T7041 32575-32580 NN denotes Assay
T7042 32564-32566 NN denotes Dc
T7043 32567-32574 NN denotes Protein
T7044 32580-32581 -RRB- denotes )
T7045 32582-32585 VBD denotes was
T7046 32596-32598 IN denotes by
T7047 32599-32602 NN denotes SDS
T7048 32603-32607 NN denotes PAGE
T7049 32602-32603 HYPH denotes -
T7050 32607-32609 , denotes ,
T7051 32609-32612 IN denotes for
T7052 32613-32623 JJ denotes subsequent
T7053 32624-32638 NN denotes immunoblotting
T7054 32638-32640 , denotes ,
T7055 32640-32642 IN denotes as
T7056 32643-32652 VBN denotes described
T7057 32653-32654 -LRB- denotes [
T7058 32654-32656 CD denotes 12
T7059 32656-32657 -RRB- denotes ]
T7060 32657-32658 . denotes .
T7061 32658-32743 sentence denotes 16.5% tris-tricine SDS-PAGE gels were used for subunit c immunoblotting experiments.
T7062 32659-32663 CD denotes 16.5
T7063 32663-32664 NN denotes %
T7064 32687-32691 NNS denotes gels
T7065 32665-32669 NN denotes tris
T7066 32670-32677 NN denotes tricine
T7067 32669-32670 HYPH denotes -
T7068 32678-32681 NN denotes SDS
T7069 32682-32686 NN denotes PAGE
T7070 32681-32682 HYPH denotes -
T7071 32697-32701 VBN denotes used
T7072 32692-32696 VBD denotes were
T7073 32702-32705 IN denotes for
T7074 32706-32713 NN denotes subunit
T7075 32714-32715 NN denotes c
T7076 32731-32742 NNS denotes experiments
T7077 32716-32730 NN denotes immunoblotting
T7078 32742-32743 . denotes .
T7116 32745-32754 NN denotes Cathepsin
T7117 32755-32756 NN denotes D
T7118 32766-32771 NN denotes assay
T7119 32757-32765 NN denotes activity
T7120 32771-33032 sentence denotes 100 mm tissue culture dishes, which were approximately 80–90% confluent, were washed briefly with ice-cold PBS, and total protein extracts were isolated by scraping cells into 10 mM Tris, pH 7.4, 0.1% Triton X-100 followed by incubation on ice, for 20 minutes.
T7121 32772-32775 CD denotes 100
T7122 32776-32778 NN denotes mm
T7123 32794-32800 NNS denotes dishes
T7124 32779-32785 NN denotes tissue
T7125 32786-32793 NN denotes culture
T7126 32850-32856 VBN denotes washed
T7127 32800-32802 , denotes ,
T7128 32802-32807 WDT denotes which
T7129 32808-32812 VBD denotes were
T7130 32813-32826 RB denotes approximately
T7131 32830-32832 CD denotes 90
T7132 32827-32829 CD denotes 80
T7133 32829-32830 SYM denotes –
T7134 32832-32833 NN denotes %
T7135 32834-32843 JJ denotes confluent
T7136 32843-32845 , denotes ,
T7137 32845-32849 VBD denotes were
T7138 32857-32864 RB denotes briefly
T7139 32865-32869 IN denotes with
T7140 32870-32873 NN denotes ice
T7141 32874-32878 JJ denotes cold
T7142 32873-32874 HYPH denotes -
T7143 32879-32882 NN denotes PBS
T7144 32882-32884 , denotes ,
T7145 32884-32887 CC denotes and
T7146 32888-32893 JJ denotes total
T7147 32902-32910 NNS denotes extracts
T7148 32894-32901 NN denotes protein
T7149 32916-32924 VBN denotes isolated
T7150 32911-32915 VBD denotes were
T7151 32925-32927 IN denotes by
T7152 32928-32936 VBG denotes scraping
T7153 32937-32942 NNS denotes cells
T7154 32943-32947 IN denotes into
T7155 32948-32950 CD denotes 10
T7156 32951-32953 NN denotes mM
T7157 32954-32958 NN denotes Tris
T7158 32958-32960 , denotes ,
T7159 32960-32962 NN denotes pH
T7160 32963-32966 CD denotes 7.4
T7161 32966-32968 , denotes ,
T7162 32968-32971 CD denotes 0.1
T7163 32971-32972 NN denotes %
T7164 32980-32981 NN denotes X
T7165 32973-32979 NN denotes Triton
T7166 32981-32982 HYPH denotes -
T7167 32982-32985 CD denotes 100
T7168 32986-32994 VBN denotes followed
T7169 32995-32997 IN denotes by
T7170 32998-33008 NN denotes incubation
T7171 33009-33011 IN denotes on
T7172 33012-33015 NN denotes ice
T7173 33015-33017 , denotes ,
T7174 33017-33020 IN denotes for
T7175 33021-33023 CD denotes 20
T7176 33024-33031 NNS denotes minutes
T7177 33031-33032 . denotes .
T7178 33032-33191 sentence denotes The insoluble material was centrifuged at 14,000 g, the supernatant was isolated, and protein concentration was determined using the Bio-rad Dc Protein Assay.
T7179 33033-33036 DT denotes The
T7180 33047-33055 NN denotes material
T7181 33037-33046 JJ denotes insoluble
T7182 33060-33071 VBN denotes centrifuged
T7183 33056-33059 VBD denotes was
T7184 33072-33074 IN denotes at
T7185 33075-33081 CD denotes 14,000
T7186 33082-33083 NN denotes g
T7187 33083-33085 , denotes ,
T7188 33085-33088 DT denotes the
T7189 33089-33100 NN denotes supernatant
T7190 33105-33113 VBN denotes isolated
T7191 33101-33104 VBD denotes was
T7192 33113-33115 , denotes ,
T7193 33115-33118 CC denotes and
T7194 33119-33126 NN denotes protein
T7195 33127-33140 NN denotes concentration
T7196 33145-33155 VBN denotes determined
T7197 33141-33144 VBD denotes was
T7198 33156-33161 VBG denotes using
T7199 33162-33165 DT denotes the
T7200 33185-33190 NN denotes Assay
T7201 33166-33169 NNP denotes Bio
T7202 33170-33173 NNP denotes rad
T7203 33169-33170 HYPH denotes -
T7204 33174-33176 NN denotes Dc
T7205 33177-33184 NN denotes Protein
T7206 33190-33191 . denotes .
T7207 33191-33411 sentence denotes 50–70 μg of total protein extract were used to measure cathepsin D activity using the Fluorogenic Innozyme™ Cathepsin D Immunocapture Activity Assay Kit (EMD Biosciences) according to the manufacturer's recommendations.
T7208 33192-33194 CD denotes 50
T7209 33195-33197 CD denotes 70
T7210 33194-33195 SYM denotes –
T7211 33198-33200 NN denotes μg
T7212 33231-33235 VBN denotes used
T7213 33201-33203 IN denotes of
T7214 33204-33209 JJ denotes total
T7215 33218-33225 NN denotes extract
T7216 33210-33217 NN denotes protein
T7217 33226-33230 VBD denotes were
T7218 33236-33238 TO denotes to
T7219 33239-33246 VB denotes measure
T7220 33247-33256 NN denotes cathepsin
T7221 33257-33258 NN denotes D
T7222 33259-33267 NN denotes activity
T7223 33268-33273 VBG denotes using
T7224 33274-33277 DT denotes the
T7225 33341-33344 NN denotes Kit
T7226 33278-33289 JJ denotes Fluorogenic
T7227 33310-33311 NN denotes D
T7228 33290-33298 NNP denotes Innozyme
T7229 33298-33299 SYM denotes ™
T7230 33300-33309 NN denotes Cathepsin
T7231 33312-33325 NN denotes Immunocapture
T7232 33326-33334 NN denotes Activity
T7233 33335-33340 NN denotes Assay
T7234 33345-33346 -LRB- denotes (
T7235 33350-33361 NNP denotes Biosciences
T7236 33346-33349 NNP denotes EMD
T7237 33361-33362 -RRB- denotes )
T7238 33363-33372 VBG denotes according
T7239 33373-33375 IN denotes to
T7240 33376-33379 DT denotes the
T7241 33380-33392 NN denotes manufacturer
T7242 33395-33410 NNS denotes recommendations
T7243 33392-33394 POS denotes 's
T7244 33410-33411 . denotes .
T7245 33411-33685 sentence denotes Relative fluorescence was measured using an Analyst AD plate reader (Molecular Devices) with the following filters and settings: excitation filter, 360-35; emission filter, 400-20, Flash lamp with 100 readings/well, 100 ms between readings, and 100,000 μs integration time.
T7246 33412-33420 JJ denotes Relative
T7247 33421-33433 NN denotes fluorescence
T7248 33438-33446 VBN denotes measured
T7249 33434-33437 VBD denotes was
T7250 33447-33452 VBG denotes using
T7251 33453-33455 DT denotes an
T7252 33473-33479 NN denotes reader
T7253 33456-33463 NN denotes Analyst
T7254 33464-33466 NN denotes AD
T7255 33467-33472 NN denotes plate
T7256 33480-33481 -LRB- denotes (
T7257 33491-33498 NNPS denotes Devices
T7258 33481-33490 JJ denotes Molecular
T7259 33498-33499 -RRB- denotes )
T7260 33500-33504 IN denotes with
T7261 33505-33508 DT denotes the
T7262 33519-33526 NNS denotes filters
T7263 33509-33518 JJ denotes following
T7264 33527-33530 CC denotes and
T7265 33531-33539 NNS denotes settings
T7266 33539-33541 : denotes :
T7267 33541-33551 NN denotes excitation
T7268 33552-33558 NN denotes filter
T7269 33564-33566 CD denotes 35
T7270 33558-33560 , denotes ,
T7271 33560-33563 CD denotes 360
T7272 33563-33564 HYPH denotes -
T7273 33566-33567 : denotes ;
T7274 33568-33576 NN denotes emission
T7275 33577-33583 NN denotes filter
T7276 33589-33591 CD denotes 20
T7277 33583-33585 , denotes ,
T7278 33585-33588 CD denotes 400
T7279 33588-33589 HYPH denotes -
T7280 33591-33593 , denotes ,
T7281 33593-33598 NN denotes Flash
T7282 33599-33603 NN denotes lamp
T7283 33604-33608 IN denotes with
T7284 33609-33612 CD denotes 100
T7285 33613-33621 NNS denotes readings
T7286 33621-33622 SYM denotes /
T7287 33622-33626 NN denotes well
T7288 33626-33628 , denotes ,
T7289 33628-33631 CD denotes 100
T7290 33632-33634 NN denotes ms
T7291 33635-33642 IN denotes between
T7292 33643-33651 NNS denotes readings
T7293 33651-33653 , denotes ,
T7294 33653-33656 CC denotes and
T7295 33657-33664 CD denotes 100,000
T7296 33665-33667 NN denotes μs
T7297 33680-33684 NN denotes time
T7298 33668-33679 NN denotes integration
T7299 33684-33685 . denotes .
T7337 33687-33696 JJ denotes Lysosomal
T7338 33697-33705 NN denotes staining
T7339 33706-33709 CC denotes and
T7340 33710-33719 JJ denotes endocytic
T7341 33720-33726 NN denotes uptake
T7342 33726-33839 sentence denotes Cells were seeded at a density of 3–5 × 104 cells per well in 4-well chamber-slides and grown overnight at 33°C.
T7343 33727-33732 NNS denotes Cells
T7344 33738-33744 VBN denotes seeded
T7345 33733-33737 VBD denotes were
T7346 33745-33747 IN denotes at
T7347 33748-33749 DT denotes a
T7348 33750-33757 NN denotes density
T7349 33758-33760 IN denotes of
T7350 33761-33762 CD denotes 3
T7351 33763-33764 CD denotes 5
T7352 33762-33763 SYM denotes –
T7353 33767-33770 CD denotes 104
T7354 33765-33766 SYM denotes ×
T7355 33771-33776 NNS denotes cells
T7356 33777-33780 IN denotes per
T7357 33781-33785 NN denotes well
T7358 33786-33788 IN denotes in
T7359 33789-33790 CD denotes 4
T7360 33791-33795 NN denotes well
T7361 33790-33791 HYPH denotes -
T7362 33804-33810 NNS denotes slides
T7363 33796-33803 NN denotes chamber
T7364 33803-33804 HYPH denotes -
T7365 33811-33814 CC denotes and
T7366 33815-33820 VBN denotes grown
T7367 33821-33830 RB denotes overnight
T7368 33831-33833 IN denotes at
T7369 33834-33836 CD denotes 33
T7370 33836-33838 NN denotes °C
T7371 33838-33839 . denotes .
T7372 33839-34033 sentence denotes Growth media was exchanged for fresh, pre-warmed growth media containing 500 nM Lysotracker or 1 mg/ml dextran-FITC, and cells were incubated at 33°C for 45 minutes or 15 minutes, respectively.
T7373 33840-33846 NN denotes Growth
T7374 33847-33852 NNS denotes media
T7375 33857-33866 VBN denotes exchanged
T7376 33853-33856 VBD denotes was
T7377 33867-33870 IN denotes for
T7378 33871-33876 JJ denotes fresh
T7379 33896-33901 NNS denotes media
T7380 33876-33878 , denotes ,
T7381 33878-33888 VBN denotes pre-warmed
T7382 33889-33895 NN denotes growth
T7383 33902-33912 VBG denotes containing
T7384 33913-33916 CD denotes 500
T7385 33917-33919 NN denotes nM
T7386 33920-33931 NNP denotes Lysotracker
T7387 33932-33934 CC denotes or
T7388 33935-33936 CD denotes 1
T7389 33937-33939 NN denotes mg
T7390 33951-33955 NN denotes FITC
T7391 33939-33940 SYM denotes /
T7392 33940-33942 NN denotes ml
T7393 33943-33950 NN denotes dextran
T7394 33950-33951 HYPH denotes -
T7395 33955-33957 , denotes ,
T7396 33957-33960 CC denotes and
T7397 33961-33966 NNS denotes cells
T7398 33972-33981 VBN denotes incubated
T7399 33967-33971 VBD denotes were
T7400 33982-33984 IN denotes at
T7401 33985-33987 CD denotes 33
T7402 33987-33989 NN denotes °C
T7403 33990-33993 IN denotes for
T7404 33994-33996 CD denotes 45
T7405 33997-34004 NNS denotes minutes
T7406 34005-34007 CC denotes or
T7407 34008-34010 CD denotes 15
T7408 34011-34018 NNS denotes minutes
T7409 34018-34020 , denotes ,
T7410 34020-34032 RB denotes respectively
T7411 34032-34033 . denotes .
T7412 34033-34206 sentence denotes Following labeling, cells were immediately placed on ice and washed for 10 minutes in ice-cold dye-free media, and fixed with 4% formaldehyde in PBS, for 20 minutes on ice.
T7413 34034-34043 VBG denotes Following
T7414 34077-34083 VBN denotes placed
T7415 34044-34052 NN denotes labeling
T7416 34052-34054 , denotes ,
T7417 34054-34059 NNS denotes cells
T7418 34060-34064 VBD denotes were
T7419 34065-34076 RB denotes immediately
T7420 34084-34086 IN denotes on
T7421 34087-34090 NN denotes ice
T7422 34091-34094 CC denotes and
T7423 34095-34101 VBN denotes washed
T7424 34102-34105 IN denotes for
T7425 34106-34108 CD denotes 10
T7426 34109-34116 NNS denotes minutes
T7427 34117-34119 IN denotes in
T7428 34120-34123 NN denotes ice
T7429 34124-34128 JJ denotes cold
T7430 34123-34124 HYPH denotes -
T7431 34138-34143 NNS denotes media
T7432 34129-34132 NN denotes dye
T7433 34133-34137 JJ denotes free
T7434 34132-34133 HYPH denotes -
T7435 34143-34145 , denotes ,
T7436 34145-34148 CC denotes and
T7437 34149-34154 VBN denotes fixed
T7438 34155-34159 IN denotes with
T7439 34160-34161 CD denotes 4
T7440 34161-34162 NN denotes %
T7441 34163-34175 NN denotes formaldehyde
T7442 34176-34178 IN denotes in
T7443 34179-34182 NN denotes PBS
T7444 34182-34184 , denotes ,
T7445 34184-34187 IN denotes for
T7446 34188-34190 CD denotes 20
T7447 34191-34198 NNS denotes minutes
T7448 34199-34201 IN denotes on
T7449 34202-34205 NN denotes ice
T7450 34205-34206 . denotes .
T7451 34206-34343 sentence denotes Chambers were removed and slides were coverslipped with Vectashield mounting media for confocal microscopy analysis, as described above.
T7452 34207-34215 NNS denotes Chambers
T7453 34221-34228 VBN denotes removed
T7454 34216-34220 VBD denotes were
T7455 34229-34232 CC denotes and
T7456 34233-34239 NNS denotes slides
T7457 34245-34257 VBN denotes coverslipped
T7458 34240-34244 VBD denotes were
T7459 34258-34262 IN denotes with
T7460 34263-34274 NNP denotes Vectashield
T7461 34284-34289 NNS denotes media
T7462 34275-34283 NN denotes mounting
T7463 34290-34293 IN denotes for
T7464 34294-34302 JJ denotes confocal
T7465 34303-34313 NN denotes microscopy
T7466 34314-34322 NN denotes analysis
T7467 34322-34324 , denotes ,
T7468 34324-34326 IN denotes as
T7469 34327-34336 VBN denotes described
T7470 34337-34342 RB denotes above
T7471 34342-34343 . denotes .
T7484 34345-34357 JJ denotes Morphometric
T7485 34358-34366 NN denotes analysis
T7486 34367-34369 IN denotes of
T7487 34370-34382 NNS denotes mitochondria
T7488 34382-34475 sentence denotes TEM photomicrographs (10,000 × – 40,000 × magnification) were taken from random grid fields.
T7489 34383-34386 NN denotes TEM
T7490 34387-34403 NNS denotes photomicrographs
T7491 34445-34450 VBN denotes taken
T7492 34404-34405 -LRB- denotes (
T7493 34425-34438 NN denotes magnification
T7494 34405-34411 CD denotes 10,000
T7495 34416-34422 CD denotes 40,000
T7496 34412-34413 SYM denotes ×
T7497 34414-34415 SYM denotes –
T7498 34423-34424 SYM denotes ×
T7499 34438-34439 -RRB- denotes )
T7500 34440-34444 VBD denotes were
T7501 34451-34455 IN denotes from
T7502 34456-34462 JJ denotes random
T7503 34468-34474 NNS denotes fields
T7504 34463-34467 NN denotes grid
T7505 34474-34475 . denotes .
T7506 34475-34712 sentence denotes For length measurements, the longest side of each mitochondria was measured in centimeters, and along the length of the mitochondria, width measurements were taken every 2.5–4 mm (dependent on the magnification of the micrograph image).
T7507 34476-34479 IN denotes For
T7508 34543-34551 VBN denotes measured
T7509 34480-34486 NN denotes length
T7510 34487-34499 NNS denotes measurements
T7511 34499-34501 , denotes ,
T7512 34501-34504 DT denotes the
T7513 34513-34517 NN denotes side
T7514 34505-34512 JJS denotes longest
T7515 34518-34520 IN denotes of
T7516 34521-34525 DT denotes each
T7517 34526-34538 NNS denotes mitochondria
T7518 34539-34542 VBD denotes was
T7519 34552-34554 IN denotes in
T7520 34555-34566 NNS denotes centimeters
T7521 34566-34568 , denotes ,
T7522 34568-34571 CC denotes and
T7523 34572-34577 IN denotes along
T7524 34634-34639 VBN denotes taken
T7525 34578-34581 DT denotes the
T7526 34582-34588 NN denotes length
T7527 34589-34591 IN denotes of
T7528 34592-34595 DT denotes the
T7529 34596-34608 NNS denotes mitochondria
T7530 34608-34610 , denotes ,
T7531 34610-34615 NN denotes width
T7532 34616-34628 NNS denotes measurements
T7533 34629-34633 VBD denotes were
T7534 34640-34645 DT denotes every
T7535 34652-34654 NN denotes mm
T7536 34646-34649 CD denotes 2.5
T7537 34650-34651 CD denotes 4
T7538 34649-34650 SYM denotes –
T7539 34655-34656 -LRB- denotes (
T7540 34656-34665 JJ denotes dependent
T7541 34666-34668 IN denotes on
T7542 34669-34672 DT denotes the
T7543 34673-34686 NN denotes magnification
T7544 34687-34689 IN denotes of
T7545 34690-34693 DT denotes the
T7546 34705-34710 NN denotes image
T7547 34694-34704 NN denotes micrograph
T7548 34710-34711 -RRB- denotes )
T7549 34711-34712 . denotes .
T7550 34712-34846 sentence denotes Following measurement, all numbers were normalized to reflect one magnification and data was analyzed using Microsoft Excel software.
T7551 34713-34722 VBG denotes Following
T7552 34753-34763 VBN denotes normalized
T7553 34723-34734 NN denotes measurement
T7554 34734-34736 , denotes ,
T7555 34736-34739 DT denotes all
T7556 34740-34747 NNS denotes numbers
T7557 34748-34752 VBD denotes were
T7558 34764-34766 TO denotes to
T7559 34767-34774 VB denotes reflect
T7560 34775-34778 CD denotes one
T7561 34779-34792 NN denotes magnification
T7562 34793-34796 CC denotes and
T7563 34797-34801 NNS denotes data
T7564 34806-34814 VBN denotes analyzed
T7565 34802-34805 VBD denotes was
T7566 34815-34820 VBG denotes using
T7567 34821-34830 NNP denotes Microsoft
T7568 34831-34836 NNP denotes Excel
T7569 34837-34845 NN denotes software
T7570 34845-34846 . denotes .
T7571 34846-34998 sentence denotes To ascertain unmagnified mitochondrial size, final measurement data, in centimeters, was converted to nanometers according to scale bar representation.
T7572 34847-34849 TO denotes To
T7573 34850-34859 VB denotes ascertain
T7574 34936-34945 VBN denotes converted
T7575 34860-34871 JJ denotes unmagnified
T7576 34886-34890 NN denotes size
T7577 34872-34885 JJ denotes mitochondrial
T7578 34890-34892 , denotes ,
T7579 34892-34897 JJ denotes final
T7580 34910-34914 NNS denotes data
T7581 34898-34909 NN denotes measurement
T7582 34914-34916 , denotes ,
T7583 34916-34918 IN denotes in
T7584 34919-34930 NNS denotes centimeters
T7585 34930-34932 , denotes ,
T7586 34932-34935 VBD denotes was
T7587 34946-34948 IN denotes to
T7588 34949-34959 NNS denotes nanometers
T7589 34960-34969 VBG denotes according
T7590 34970-34972 IN denotes to
T7591 34973-34978 NN denotes scale
T7592 34979-34982 NN denotes bar
T7593 34983-34997 NN denotes representation
T7594 34997-34998 . denotes .
T7619 35000-35003 NN denotes ATP
T7620 35004-35015 NN denotes measurement
T7621 35015-35151 sentence denotes ATP was measured by using the CellTiter-GLO® Luminescent Cell Viability kit (Promega), according to the manufacturer's recommendations.
T7622 35016-35019 NN denotes ATP
T7623 35024-35032 VBN denotes measured
T7624 35020-35023 VBD denotes was
T7625 35033-35035 IN denotes by
T7626 35036-35041 VBG denotes using
T7627 35042-35045 DT denotes the
T7628 35088-35091 NN denotes kit
T7629 35046-35055 NNP denotes CellTiter
T7630 35056-35060 NNP denotes GLO®
T7631 35055-35056 HYPH denotes -
T7632 35061-35072 JJ denotes Luminescent
T7633 35073-35077 NN denotes Cell
T7634 35078-35087 NN denotes Viability
T7635 35092-35093 -LRB- denotes (
T7636 35093-35100 NNP denotes Promega
T7637 35100-35101 -RRB- denotes )
T7638 35101-35103 , denotes ,
T7639 35103-35112 VBG denotes according
T7640 35113-35115 IN denotes to
T7641 35116-35119 DT denotes the
T7642 35120-35132 NN denotes manufacturer
T7643 35135-35150 NNS denotes recommendations
T7644 35132-35134 POS denotes 's
T7645 35150-35151 . denotes .
T7646 35151-35298 sentence denotes Briefly, cells were plated in a black opaque-walled 96 well plate (Packard Bioscience) at a density of 20000/well and incubated at 33°C overnight.
T7647 35152-35159 RB denotes Briefly
T7648 35172-35178 VBN denotes plated
T7649 35159-35161 , denotes ,
T7650 35161-35166 NNS denotes cells
T7651 35167-35171 VBD denotes were
T7652 35179-35181 IN denotes in
T7653 35182-35183 DT denotes a
T7654 35212-35217 NN denotes plate
T7655 35184-35189 JJ denotes black
T7656 35190-35196 JJ denotes opaque
T7657 35197-35203 JJ denotes walled
T7658 35196-35197 HYPH denotes -
T7659 35204-35206 CD denotes 96
T7660 35207-35211 NN denotes well
T7661 35218-35219 -LRB- denotes (
T7662 35227-35237 NNP denotes Bioscience
T7663 35219-35226 NNP denotes Packard
T7664 35237-35238 -RRB- denotes )
T7665 35239-35241 IN denotes at
T7666 35242-35243 DT denotes a
T7667 35244-35251 NN denotes density
T7668 35252-35254 IN denotes of
T7669 35255-35260 CD denotes 20000
T7670 35260-35261 SYM denotes /
T7671 35261-35265 NN denotes well
T7672 35266-35269 CC denotes and
T7673 35270-35279 VBN denotes incubated
T7674 35280-35282 IN denotes at
T7675 35283-35285 CD denotes 33
T7676 35285-35287 NN denotes °C
T7677 35288-35297 RB denotes overnight
T7678 35297-35298 . denotes .
T7679 35298-35411 sentence denotes The following day, CellTiter-GLO® Reagent was added to each well and cell lysis was induced by mixing 2 minutes.
T7680 35299-35302 DT denotes The
T7681 35313-35316 NN denotes day
T7682 35303-35312 JJ denotes following
T7683 35345-35350 VBN denotes added
T7684 35316-35318 , denotes ,
T7685 35318-35327 NNP denotes CellTiter
T7686 35328-35332 NNP denotes GLO®
T7687 35327-35328 HYPH denotes -
T7688 35333-35340 NN denotes Reagent
T7689 35341-35344 VBD denotes was
T7690 35351-35353 IN denotes to
T7691 35354-35358 DT denotes each
T7692 35359-35363 NN denotes well
T7693 35364-35367 CC denotes and
T7694 35368-35372 NN denotes cell
T7695 35373-35378 NN denotes lysis
T7696 35383-35390 VBN denotes induced
T7697 35379-35382 VBD denotes was
T7698 35391-35393 IN denotes by
T7699 35394-35400 VBG denotes mixing
T7700 35401-35402 CD denotes 2
T7701 35403-35410 NNS denotes minutes
T7702 35410-35411 . denotes .
T7703 35411-35465 sentence denotes An ATP standard curve was prepared in the same plate.
T7704 35412-35414 DT denotes An
T7705 35428-35433 NN denotes curve
T7706 35415-35418 NN denotes ATP
T7707 35419-35427 JJ denotes standard
T7708 35438-35446 VBN denotes prepared
T7709 35434-35437 VBD denotes was
T7710 35447-35449 IN denotes in
T7711 35450-35453 DT denotes the
T7712 35459-35464 NN denotes plate
T7713 35454-35458 JJ denotes same
T7714 35464-35465 . denotes .
T7715 35465-35675 sentence denotes Before recording luminescence with a microplate luminometer (MicroLumat Plus LB 96V, Berthold Techonologies), the plate was dark adapted for 10 minutes at room temperature to stabilize the luminescence signal.
T7716 35466-35472 IN denotes Before
T7717 35595-35602 VBN denotes adapted
T7718 35473-35482 VBG denotes recording
T7719 35483-35495 NN denotes luminescence
T7720 35496-35500 IN denotes with
T7721 35501-35502 DT denotes a
T7722 35514-35525 NN denotes luminometer
T7723 35503-35513 NN denotes microplate
T7724 35526-35527 -LRB- denotes (
T7725 35527-35537 NNP denotes MicroLumat
T7726 35546-35549 NNP denotes 96V
T7727 35538-35542 NNP denotes Plus
T7728 35543-35545 NNP denotes LB
T7729 35549-35551 , denotes ,
T7730 35560-35573 NNP denotes Techonologies
T7731 35551-35559 NNP denotes Berthold
T7732 35573-35574 -RRB- denotes )
T7733 35574-35576 , denotes ,
T7734 35576-35579 DT denotes the
T7735 35580-35585 NN denotes plate
T7736 35586-35589 VBD denotes was
T7737 35590-35594 NN denotes dark
T7738 35603-35606 IN denotes for
T7739 35607-35609 CD denotes 10
T7740 35610-35617 NNS denotes minutes
T7741 35618-35620 IN denotes at
T7742 35621-35625 NN denotes room
T7743 35626-35637 NN denotes temperature
T7744 35638-35640 TO denotes to
T7745 35641-35650 VB denotes stabilize
T7746 35651-35654 DT denotes the
T7747 35668-35674 NN denotes signal
T7748 35655-35667 NN denotes luminescence
T7749 35674-35675 . denotes .
T7780 35677-35685 NN denotes Hydrogen
T7781 35686-35694 NN denotes peroxide
T7782 35695-35704 NN denotes treatment
T7783 35705-35710 NN denotes assay
T7784 35710-35813 sentence denotes Cells were plated at a density of 10,000 cells/well in 96-well plates and incubated at 33°C overnight.
T7785 35711-35716 NNS denotes Cells
T7786 35722-35728 VBN denotes plated
T7787 35717-35721 VBD denotes were
T7788 35729-35731 IN denotes at
T7789 35732-35733 DT denotes a
T7790 35734-35741 NN denotes density
T7791 35742-35744 IN denotes of
T7792 35745-35751 CD denotes 10,000
T7793 35752-35757 NNS denotes cells
T7794 35757-35758 SYM denotes /
T7795 35758-35762 NN denotes well
T7796 35763-35765 IN denotes in
T7797 35766-35768 CD denotes 96
T7798 35769-35773 NN denotes well
T7799 35768-35769 HYPH denotes -
T7800 35774-35780 NNS denotes plates
T7801 35781-35784 CC denotes and
T7802 35785-35794 VBN denotes incubated
T7803 35795-35797 IN denotes at
T7804 35798-35800 CD denotes 33
T7805 35800-35802 NN denotes °C
T7806 35803-35812 RB denotes overnight
T7807 35812-35813 . denotes .
T7808 35813-35927 sentence denotes The following day, fresh media containing varying concentrations of hydrogen peroxide was dispersed to each well.
T7809 35814-35817 DT denotes The
T7810 35828-35831 NN denotes day
T7811 35818-35827 VBG denotes following
T7812 35904-35913 VBN denotes dispersed
T7813 35831-35833 , denotes ,
T7814 35833-35838 JJ denotes fresh
T7815 35839-35844 NNS denotes media
T7816 35845-35855 VBG denotes containing
T7817 35856-35863 VBG denotes varying
T7818 35864-35878 NNS denotes concentrations
T7819 35879-35881 IN denotes of
T7820 35882-35890 NN denotes hydrogen
T7821 35891-35899 NN denotes peroxide
T7822 35900-35903 VBD denotes was
T7823 35914-35916 IN denotes to
T7824 35917-35921 DT denotes each
T7825 35922-35926 NN denotes well
T7826 35926-35927 . denotes .
T7827 35927-36167 sentence denotes Cells were incubated in the presence of hydrogen peroxide for 24 hours, at 33°C, and viability was measured using the CellTiter-96® AQueous Non-Radioactive Cell Proliferation Assay (Promega), according to the manufacturer's specifications.
T7828 35928-35933 NNS denotes Cells
T7829 35939-35948 VBN denotes incubated
T7830 35934-35938 VBD denotes were
T7831 35949-35951 IN denotes in
T7832 35952-35955 DT denotes the
T7833 35956-35964 NN denotes presence
T7834 35965-35967 IN denotes of
T7835 35968-35976 NN denotes hydrogen
T7836 35977-35985 NN denotes peroxide
T7837 35986-35989 IN denotes for
T7838 35990-35992 CD denotes 24
T7839 35993-35998 NNS denotes hours
T7840 35998-36000 , denotes ,
T7841 36000-36002 IN denotes at
T7842 36003-36005 CD denotes 33
T7843 36005-36007 NN denotes °C
T7844 36007-36009 , denotes ,
T7845 36009-36012 CC denotes and
T7846 36013-36022 NN denotes viability
T7847 36027-36035 VBN denotes measured
T7848 36023-36026 VBD denotes was
T7849 36036-36041 VBG denotes using
T7850 36042-36045 DT denotes the
T7851 36103-36108 NN denotes Assay
T7852 36046-36055 NNP denotes CellTiter
T7853 36056-36059 NN denotes 96®
T7854 36055-36056 HYPH denotes -
T7855 36060-36067 JJ denotes AQueous
T7856 36068-36083 JJ denotes Non-Radioactive
T7857 36084-36088 NN denotes Cell
T7858 36089-36102 NN denotes Proliferation
T7859 36109-36110 -LRB- denotes (
T7860 36110-36117 NNP denotes Promega
T7861 36117-36118 -RRB- denotes )
T7862 36118-36120 , denotes ,
T7863 36120-36129 VBG denotes according
T7864 36130-36132 IN denotes to
T7865 36133-36136 DT denotes the
T7866 36137-36149 NN denotes manufacturer
T7867 36152-36166 NNS denotes specifications
T7868 36149-36151 POS denotes 's
T7869 36166-36167 . denotes .
T7982 5427-5435 JJ denotes Neuronal
T7983 5436-5442 NN denotes marker
T7984 5443-5453 NN denotes expression
T7985 5557-5562 VBN denotes shown
T7986 5454-5456 IN denotes in
T7987 5457-5463 NN denotes CbCln3
T7988 5467-5472 NNS denotes cells
T7989 5463-5464 SYM denotes +
T7990 5464-5465 HYPH denotes /
T7991 5465-5466 SYM denotes +
T7992 5473-5489 NN denotes Characterization
T7993 5490-5492 IN denotes of
T7994 5493-5499 NN denotes CbCln3
T7995 5503-5508 NNS denotes cells
T7996 5499-5500 SYM denotes +
T7997 5500-5501 HYPH denotes /
T7998 5501-5502 SYM denotes +
T7999 5509-5511 IN denotes by
T8000 5512-5530 NN denotes immunofluorescence
T8001 5531-5535 IN denotes with
T8002 5536-5542 NN denotes marker
T8003 5543-5553 NNS denotes antibodies
T8004 5554-5556 VBZ denotes is
T8005 5562-5563 . denotes .
T8006 5563-5690 sentence denotes CbCln3+/+ precursors exhibit nestin expression (a) but not GFAP expression (b), consistent with a neuronal precursor identity.
T8007 5564-5570 NN denotes CbCln3
T8008 5574-5584 NNS denotes precursors
T8009 5570-5571 SYM denotes +
T8010 5571-5572 HYPH denotes /
T8011 5572-5573 SYM denotes +
T8012 5585-5592 VBP denotes exhibit
T8013 5593-5599 NN denotes nestin
T8014 5600-5610 NN denotes expression
T8015 5611-5612 -LRB- denotes (
T8016 5612-5613 NN denotes a
T8017 5613-5614 -RRB- denotes )
T8018 5615-5618 CC denotes but
T8019 5619-5622 RB denotes not
T8020 5623-5627 NN denotes GFAP
T8021 5628-5638 NN denotes expression
T8022 5639-5640 -LRB- denotes (
T8023 5640-5641 NN denotes b
T8024 5641-5642 -RRB- denotes )
T8025 5642-5644 , denotes ,
T8026 5644-5654 JJ denotes consistent
T8027 5655-5659 IN denotes with
T8028 5660-5661 DT denotes a
T8029 5681-5689 NN denotes identity
T8030 5662-5670 JJ denotes neuronal
T8031 5671-5680 NN denotes precursor
T8032 5689-5690 . denotes .
T8033 5690-5908 sentence denotes Upon stimulation with a differentiation cocktail (see Methods), CbCln3+/+ cells achieved neuron-like morphology, with rounded cell bodies and extension of processes, and MAP2 (c) and NeuN (d) expression was increased.
T8034 5691-5695 IN denotes Upon
T8035 5771-5779 VBD denotes achieved
T8036 5696-5707 NN denotes stimulation
T8037 5708-5712 IN denotes with
T8038 5713-5714 DT denotes a
T8039 5731-5739 NN denotes cocktail
T8040 5715-5730 NN denotes differentiation
T8041 5740-5741 -LRB- denotes (
T8042 5741-5744 VB denotes see
T8043 5745-5752 NNS denotes Methods
T8044 5752-5753 -RRB- denotes )
T8045 5753-5755 , denotes ,
T8046 5755-5761 NN denotes CbCln3
T8047 5765-5770 NNS denotes cells
T8048 5761-5762 SYM denotes +
T8049 5762-5763 HYPH denotes /
T8050 5763-5764 SYM denotes +
T8051 5780-5786 NN denotes neuron
T8052 5787-5791 JJ denotes like
T8053 5786-5787 HYPH denotes -
T8054 5792-5802 NN denotes morphology
T8055 5802-5804 , denotes ,
T8056 5804-5808 IN denotes with
T8057 5809-5816 JJ denotes rounded
T8058 5822-5828 NNS denotes bodies
T8059 5817-5821 NN denotes cell
T8060 5829-5832 CC denotes and
T8061 5833-5842 NN denotes extension
T8062 5843-5845 IN denotes of
T8063 5846-5855 NNS denotes processes
T8064 5855-5857 , denotes ,
T8065 5857-5860 CC denotes and
T8066 5861-5865 NN denotes MAP2
T8067 5883-5893 NN denotes expression
T8068 5866-5867 -LRB- denotes (
T8069 5867-5868 NN denotes c
T8070 5868-5869 -RRB- denotes )
T8071 5870-5873 CC denotes and
T8072 5874-5878 NN denotes NeuN
T8073 5879-5880 -LRB- denotes (
T8074 5880-5881 NN denotes d
T8075 5881-5882 -RRB- denotes )
T8076 5898-5907 VBN denotes increased
T8077 5894-5897 VBD denotes was
T8078 5907-5908 . denotes .
T8079 5908-5983 sentence denotes CbCln3+/+ cells are negative for the Purkinje neuron marker calbindin (e).
T8080 5909-5915 NN denotes CbCln3
T8081 5919-5924 NNS denotes cells
T8082 5915-5916 SYM denotes +
T8083 5916-5917 HYPH denotes /
T8084 5917-5918 SYM denotes +
T8085 5925-5928 VBP denotes are
T8086 5929-5937 JJ denotes negative
T8087 5938-5941 IN denotes for
T8088 5942-5945 DT denotes the
T8089 5962-5968 NN denotes marker
T8090 5946-5954 NNP denotes Purkinje
T8091 5955-5961 NN denotes neuron
T8092 5969-5978 NN denotes calbindin
T8093 5979-5980 -LRB- denotes (
T8094 5980-5981 NN denotes e
T8095 5981-5982 -RRB- denotes )
T8096 5982-5983 . denotes .
T8097 5983-6088 sentence denotes CbCln3+/Δex7/8 and CbCln3Δex7/8/Δex7/8 cell lines exhibited identical marker immunofluorescence results.
T8098 5984-5990 NN denotes CbCln3
T8099 5992-5996 NN denotes Δex7
T8100 5990-5991 SYM denotes +
T8101 5991-5992 HYPH denotes /
T8102 6028-6033 NNS denotes lines
T8103 5996-5997 HYPH denotes /
T8104 5997-5998 CD denotes 8
T8105 5999-6002 CC denotes and
T8106 6003-6013 NN denotes CbCln3Δex7
T8107 6016-6020 NN denotes Δex7
T8108 6013-6014 HYPH denotes /
T8109 6014-6015 CD denotes 8
T8110 6015-6016 HYPH denotes /
T8111 6020-6021 HYPH denotes /
T8112 6021-6022 CD denotes 8
T8113 6023-6027 NN denotes cell
T8114 6034-6043 VBD denotes exhibited
T8115 6044-6053 JJ denotes identical
T8116 6080-6087 NNS denotes results
T8117 6054-6060 NN denotes marker
T8118 6061-6079 NN denotes immunofluorescence
T8119 6087-6088 . denotes .
T8120 6088-6143 sentence denotes a, b) 20 × magnification; c, d, e) 40 × magnification.
T8121 6089-6090 LS denotes a
T8122 6100-6113 NN denotes magnification
T8123 6090-6092 , denotes ,
T8124 6092-6093 LS denotes b
T8125 6093-6094 -RRB- denotes )
T8126 6095-6097 CD denotes 20
T8127 6098-6099 SYM denotes ×
T8128 6113-6114 : denotes ;
T8129 6115-6116 LS denotes c
T8130 6129-6142 NN denotes magnification
T8131 6116-6118 , denotes ,
T8132 6118-6119 LS denotes d
T8133 6119-6121 , denotes ,
T8134 6121-6122 LS denotes e
T8135 6122-6123 -RRB- denotes )
T8136 6124-6126 CD denotes 40
T8137 6127-6128 SYM denotes ×
T8138 6142-6143 . denotes .
T8230 7870-7871 HYPH denotes /
T8215 7812-7814 NN denotes RT
T8216 7815-7818 NN denotes PCR
T8217 7814-7815 HYPH denotes -
T8218 7935-7940 VBN denotes shown
T8219 7819-7821 IN denotes of
T8220 7822-7826 NN denotes Cln3
T8221 7827-7831 NN denotes mRNA
T8222 7832-7834 IN denotes in
T8223 7835-7839 JJ denotes wild
T8224 7840-7844 NN denotes type
T8225 7839-7840 HYPH denotes -
T8226 7873-7878 NNS denotes cells
T8227 7845-7848 CC denotes and
T8228 7849-7859 JJ denotes homozygous
T8229 7860-7870 NN denotes CbCln3Δex7
T8231 7871-7872 CD denotes 8
T8232 7879-7883 NN denotes Cln3
T8233 7922-7930 NNS denotes products
T8234 7884-7889 NN denotes Exon1
T8235 7890-7897 JJ denotes forward
T8236 7889-7890 HYPH denotes -
T8237 7907-7914 JJ denotes reverse
T8238 7897-7899 , denotes ,
T8239 7899-7903 NN denotes Exon
T8240 7904-7906 CD denotes 15
T8241 7906-7907 HYPH denotes -
T8242 7915-7917 NN denotes RT
T8243 7918-7921 NN denotes PCR
T8244 7917-7918 HYPH denotes -
T8245 7931-7934 VBP denotes are
T8246 7940-7942 , denotes ,
T8247 7942-7946 IN denotes from
T8248 7947-7952 JJ denotes total
T8249 8026-8029 NN denotes RNA
T8250 7953-7957 JJ denotes wild
T8251 7958-7962 NN denotes type
T8252 7957-7958 HYPH denotes -
T8253 7963-7964 -LRB- denotes (
T8254 7964-7965 SYM denotes +
T8255 7966-7967 SYM denotes +
T8256 7965-7966 HYPH denotes /
T8257 7967-7968 -RRB- denotes )
T8258 7969-7971 CC denotes or
T8259 7972-7982 JJ denotes homozygous
T8260 7983-7989 NN denotes mutant
T8261 7990-7991 -LRB- denotes (
T8262 7991-7995 NN denotes Δex7
T8263 7995-7996 HYPH denotes /
T8264 7996-7997 CD denotes 8
T8265 7997-7998 HYPH denotes /
T8266 7998-8002 NN denotes Δex7
T8267 8002-8003 HYPH denotes /
T8268 8003-8004 CD denotes 8
T8269 8004-8005 -RRB- denotes )
T8270 8006-8011 NN denotes brain
T8271 8012-8015 CC denotes and
T8272 8016-8020 NN denotes cell
T8273 8021-8025 NN denotes line
T8274 8029-8030 . denotes .
T8275 8030-8145 sentence denotes Brain and cell line RT-PCR reaction products had identical band patterns on ethidium-bromide stained agarose gels.
T8276 8031-8036 NN denotes Brain
T8277 8067-8075 NNS denotes products
T8278 8037-8040 CC denotes and
T8279 8041-8045 NN denotes cell
T8280 8046-8050 NN denotes line
T8281 8051-8053 NN denotes RT
T8282 8054-8057 NN denotes PCR
T8283 8053-8054 HYPH denotes -
T8284 8058-8066 NN denotes reaction
T8285 8076-8079 VBD denotes had
T8286 8080-8089 JJ denotes identical
T8287 8095-8103 NNS denotes patterns
T8288 8090-8094 NN denotes band
T8289 8104-8106 IN denotes on
T8290 8107-8115 NN denotes ethidium
T8291 8116-8123 NN denotes bromide
T8292 8115-8116 HYPH denotes -
T8293 8124-8131 VBN denotes stained
T8294 8140-8144 NNS denotes gels
T8295 8132-8139 NN denotes agarose
T8296 8144-8145 . denotes .
T8297 8145-8305 sentence denotes Wild-type RT-PCR product was a single ~1.6 kb band and mutant products were ~1.6, ~1.5, ~1.4, ~1.35, and ~1.3 kb, representing multiple mutant splice variants.
T8298 8146-8150 JJ denotes Wild
T8299 8151-8155 NN denotes type
T8300 8150-8151 HYPH denotes -
T8301 8163-8170 NN denotes product
T8302 8156-8158 NN denotes RT
T8303 8159-8162 NN denotes PCR
T8304 8158-8159 HYPH denotes -
T8305 8171-8174 VBD denotes was
T8306 8175-8176 DT denotes a
T8307 8192-8196 NN denotes band
T8308 8177-8183 JJ denotes single
T8309 8184-8185 SYM denotes ~
T8310 8185-8188 CD denotes 1.6
T8311 8189-8191 NN denotes kb
T8312 8197-8200 CC denotes and
T8313 8201-8207 NN denotes mutant
T8314 8208-8216 NNS denotes products
T8315 8217-8221 VBD denotes were
T8316 8222-8223 SYM denotes ~
T8317 8223-8226 CD denotes 1.6
T8318 8256-8258 NN denotes kb
T8319 8226-8228 , denotes ,
T8320 8228-8229 SYM denotes ~
T8321 8229-8232 CD denotes 1.5
T8322 8232-8234 , denotes ,
T8323 8234-8235 SYM denotes ~
T8324 8235-8238 CD denotes 1.4
T8325 8238-8240 , denotes ,
T8326 8240-8241 SYM denotes ~
T8327 8241-8245 CD denotes 1.35
T8328 8245-8247 , denotes ,
T8329 8247-8250 CC denotes and
T8330 8251-8252 SYM denotes ~
T8331 8252-8255 NN denotes 1.3
T8332 8258-8260 , denotes ,
T8333 8260-8272 VBG denotes representing
T8334 8273-8281 JJ denotes multiple
T8335 8296-8304 NNS denotes variants
T8336 8282-8288 NN denotes mutant
T8337 8289-8295 NN denotes splice
T8338 8304-8305 . denotes .
T8445 8316-8324 NN denotes Battenin
T8446 8353-8359 NN denotes marker
T8447 8325-8328 CC denotes and
T8448 8329-8338 JJ denotes lysosomal
T8449 8339-8342 CC denotes and
T8450 8343-8352 JJ denotes endosomal
T8451 8360-8371 NN denotes co-staining
T8452 8560-8565 VBN denotes shown
T8453 8372-8374 IN denotes in
T8454 8375-8379 JJ denotes wild
T8455 8380-8384 NN denotes type
T8456 8379-8380 HYPH denotes -
T8457 8434-8439 NNS denotes cells
T8458 8385-8388 CC denotes and
T8459 8389-8399 JJ denotes homozygous
T8460 8400-8410 NN denotes CbCln3Δex7
T8461 8424-8433 NN denotes precursor
T8462 8410-8411 HYPH denotes /
T8463 8411-8412 CD denotes 8
T8464 8413-8423 JJ denotes cerebellar
T8465 8440-8445 NN denotes Batp1
T8466 8446-8460 NN denotes immunostaining
T8467 8461-8463 IN denotes of
T8468 8464-8468 JJ denotes wild
T8469 8469-8473 NN denotes type
T8470 8468-8469 HYPH denotes -
T8471 8474-8475 -LRB- denotes (
T8472 8475-8481 NN denotes CbCln3
T8473 8481-8482 SYM denotes +
T8474 8482-8483 HYPH denotes /
T8475 8483-8484 SYM denotes +
T8476 8484-8485 -RRB- denotes )
T8477 8486-8489 CC denotes and
T8478 8490-8500 JJ denotes homozygous
T8479 8501-8507 NN denotes mutant
T8480 8551-8556 NNS denotes cells
T8481 8508-8509 -LRB- denotes (
T8482 8509-8519 NN denotes CbCln3Δex7
T8483 8522-8526 NN denotes Δex7
T8484 8519-8520 HYPH denotes /
T8485 8520-8521 CD denotes 8
T8486 8521-8522 HYPH denotes /
T8487 8526-8527 HYPH denotes /
T8488 8527-8528 CD denotes 8
T8489 8528-8529 -RRB- denotes )
T8490 8530-8540 JJ denotes cerebellar
T8491 8541-8550 NN denotes precursor
T8492 8557-8559 VBZ denotes is
T8493 8565-8567 , denotes ,
T8494 8567-8571 IN denotes with
T8495 8572-8583 NN denotes co-staining
T8496 8584-8587 IN denotes for
T8497 8588-8597 NNS denotes lysosomes
T8498 8598-8599 -LRB- denotes (
T8499 8599-8603 NN denotes Lamp
T8500 8604-8605 CD denotes 1
T8501 8605-8606 -RRB- denotes )
T8502 8606-8608 , denotes ,
T8503 8608-8613 JJ denotes early
T8504 8614-8623 NNS denotes endosomes
T8505 8624-8625 -LRB- denotes (
T8506 8625-8629 NN denotes EEA1
T8507 8629-8630 -RRB- denotes )
T8508 8630-8632 , denotes ,
T8509 8632-8635 CC denotes and
T8510 8636-8640 JJ denotes late
T8511 8641-8650 NNS denotes endosomes
T8512 8651-8652 -LRB- denotes (
T8513 8652-8656 NN denotes Rab7
T8514 8656-8657 -RRB- denotes )
T8515 8657-8658 . denotes .
T8516 8658-8829 sentence denotes Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge).
T8517 8659-8670 JJ denotes Significant
T8518 8671-8678 NN denotes overlap
T8519 8773-8777 VBN denotes seen
T8520 8679-8681 IN denotes of
T8521 8682-8687 NN denotes Batp1
T8522 8688-8694 NN denotes signal
T8523 8695-8696 -LRB- denotes (
T8524 8696-8699 JJ denotes red
T8525 8699-8700 -RRB- denotes )
T8526 8701-8705 IN denotes with
T8527 8706-8710 NN denotes EEA1
T8528 8711-8712 -LRB- denotes (
T8529 8726-8732 NNS denotes panels
T8530 8712-8717 JJ denotes green
T8531 8717-8719 , denotes ,
T8532 8719-8725 JJ denotes middle
T8533 8732-8733 -RRB- denotes )
T8534 8734-8737 CC denotes and
T8535 8738-8742 NN denotes Rab7
T8536 8743-8744 -LRB- denotes (
T8537 8758-8764 NNS denotes panels
T8538 8744-8749 JJ denotes green
T8539 8749-8751 , denotes ,
T8540 8751-8757 JJ denotes bottom
T8541 8764-8765 -RRB- denotes )
T8542 8766-8769 MD denotes can
T8543 8770-8772 VB denotes be
T8544 8778-8780 IN denotes as
T8545 8781-8787 JJ denotes yellow
T8546 8788-8792 WRB denotes when
T8547 8814-8820 VBN denotes merged
T8548 8793-8796 DT denotes the
T8549 8801-8809 NNS denotes channels
T8550 8797-8800 CD denotes two
T8551 8810-8813 VBP denotes are
T8552 8821-8822 -LRB- denotes (
T8553 8822-8827 NNP denotes Merge
T8554 8827-8828 -RRB- denotes )
T8555 8828-8829 . denotes .
T8556 8829-8880 sentence denotes The degree of Batp1 overlap is greatest with Rab7.
T8557 8830-8833 DT denotes The
T8558 8834-8840 NN denotes degree
T8559 8858-8860 VBZ denotes is
T8560 8841-8843 IN denotes of
T8561 8844-8849 NN denotes Batp1
T8562 8850-8857 NN denotes overlap
T8563 8861-8869 JJS denotes greatest
T8564 8870-8874 IN denotes with
T8565 8875-8879 NN denotes Rab7
T8566 8879-8880 . denotes .
T8567 8880-8965 sentence denotes Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen.
T8568 8881-8885 RB denotes Only
T8569 8894-8901 NN denotes overlap
T8570 8886-8893 VBN denotes limited
T8571 8960-8964 VBN denotes seen
T8572 8902-8909 IN denotes between
T8573 8910-8915 NN denotes Batp1
T8574 8916-8917 -LRB- denotes (
T8575 8917-8920 JJ denotes red
T8576 8920-8921 -RRB- denotes )
T8577 8922-8925 CC denotes and
T8578 8926-8930 NN denotes Lamp
T8579 8931-8932 CD denotes 1
T8580 8933-8934 -LRB- denotes (
T8581 8945-8951 NNS denotes panels
T8582 8934-8939 JJ denotes green
T8583 8939-8941 , denotes ,
T8584 8941-8944 JJ denotes top
T8585 8951-8952 -RRB- denotes )
T8586 8953-8956 MD denotes can
T8587 8957-8959 VB denotes be
T8588 8964-8965 . denotes .
T8589 8965-9168 sentence denotes Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels.
T8590 8966-8971 NN denotes Batp1
T8591 8972-8978 NN denotes signal
T8592 9012-9014 VBZ denotes is
T8593 8979-8981 IN denotes in
T8594 8982-8992 JJ denotes homozygous
T8595 9006-9011 NNS denotes cells
T8596 8993-9003 NN denotes CbCln3Δex7
T8597 9003-9004 HYPH denotes /
T8598 9004-9005 CD denotes 8
T8599 9015-9028 RB denotes significantly
T8600 9029-9036 JJ denotes reduced
T8601 9036-9038 , denotes ,
T8602 9038-9041 CC denotes but
T8603 9042-9053 JJ denotes significant
T8604 9054-9061 NN denotes overlap
T8605 9121-9125 VBN denotes seen
T8606 9062-9066 IN denotes with
T8607 9067-9071 NN denotes EEA1
T8608 9072-9075 CC denotes and
T8609 9076-9080 NN denotes Rab7
T8610 9080-9082 , denotes ,
T8611 9082-9085 CC denotes and
T8612 9086-9090 RB denotes very
T8613 9091-9097 JJ denotes little
T8614 9105-9112 NN denotes overlap
T8615 9098-9102 NNP denotes Lamp
T8616 9103-9104 CD denotes 1
T8617 9112-9114 , denotes ,
T8618 9114-9117 MD denotes can
T8619 9118-9120 VB denotes be
T8620 9126-9128 IN denotes as
T8621 9129-9135 JJ denotes yellow
T8622 9136-9138 IN denotes in
T8623 9139-9142 DT denotes the
T8624 9161-9167 NNS denotes panels
T8625 9143-9153 JJ denotes respective
T8626 9154-9160 JJ denotes merged
T8627 9167-9168 . denotes .
T8628 9168-9302 sentence denotes Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells.
T8629 9169-9176 RB denotes Notably
T8630 9207-9213 VBP denotes appear
T8631 9176-9178 , denotes ,
T8632 9178-9182 NN denotes Lamp
T8633 9194-9206 NN denotes localization
T8634 9183-9184 CD denotes 1
T8635 9185-9188 CC denotes and
T8636 9189-9193 NN denotes EEA1
T8637 9214-9221 JJ denotes altered
T8638 9221-9223 , denotes ,
T8639 9223-9226 CC denotes and
T8640 9227-9231 NN denotes Rab7
T8641 9232-9240 NN denotes staining
T8642 9241-9244 VBD denotes was
T8643 9245-9255 RB denotes frequently
T8644 9256-9260 RBR denotes less
T8645 9261-9268 JJ denotes intense
T8646 9269-9271 IN denotes in
T8647 9272-9282 JJ denotes homozygous
T8648 9296-9301 NNS denotes cells
T8649 9283-9293 NN denotes CbCln3Δex7
T8650 9293-9294 HYPH denotes /
T8651 9294-9295 CD denotes 8
T8652 9301-9302 . denotes .
T8653 9302-9404 sentence denotes Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings.
T8654 9303-9307 JJ denotes Wild
T8655 9308-9312 NN denotes type
T8656 9307-9308 HYPH denotes -
T8657 9362-9370 VBN denotes captured
T8658 9313-9316 CC denotes and
T8659 9317-9327 JJ denotes homozygous
T8660 9328-9338 NN denotes CbCln3Δex7
T8661 9350-9356 NNS denotes images
T8662 9338-9339 HYPH denotes /
T8663 9339-9340 CD denotes 8
T8664 9341-9349 JJ denotes confocal
T8665 9357-9361 VBD denotes were
T8666 9371-9375 IN denotes with
T8667 9376-9385 JJ denotes identical
T8668 9395-9403 NNS denotes settings
T8669 9386-9394 NN denotes exposure
T8670 9403-9404 . denotes .
T8671 9404-9424 sentence denotes 60 × magnification.
T8672 9405-9407 CD denotes 60
T8673 9410-9423 NN denotes magnification
T8674 9408-9409 SYM denotes ×
T8675 9423-9424 . denotes .
T8805 10430-10437 NN denotes Subunit
T8806 10438-10439 NN denotes c
T8807 10440-10452 NN denotes accumulation
T8808 10453-10455 IN denotes in
T8809 10456-10466 JJ denotes homozygous
T8810 10501-10506 NNS denotes cells
T8811 10467-10477 NN denotes CbCln3Δex7
T8812 10477-10478 HYPH denotes /
T8813 10478-10479 CD denotes 8
T8814 10480-10490 JJ denotes cerebellar
T8815 10491-10500 NN denotes precursor
T8816 10506-10634 sentence denotes a. Subunit c immunostaining and autofluorescence of 7-day confluency-aged wild-type and homozygous CbCln3Δex7/8 cells is shown.
T8817 10507-10508 LS denotes a
T8818 10628-10633 VBN denotes shown
T8819 10508-10509 . denotes .
T8820 10510-10517 NN denotes Subunit
T8821 10518-10519 NN denotes c
T8822 10520-10534 NN denotes immunostaining
T8823 10535-10538 CC denotes and
T8824 10539-10555 NN denotes autofluorescence
T8825 10556-10558 IN denotes of
T8826 10559-10560 CD denotes 7
T8827 10561-10564 NN denotes day
T8828 10560-10561 HYPH denotes -
T8829 10565-10575 NN denotes confluency
T8830 10576-10580 VBN denotes aged
T8831 10575-10576 HYPH denotes -
T8832 10619-10624 NNS denotes cells
T8833 10581-10585 JJ denotes wild
T8834 10586-10590 NN denotes type
T8835 10585-10586 HYPH denotes -
T8836 10591-10594 CC denotes and
T8837 10595-10605 JJ denotes homozygous
T8838 10606-10616 NN denotes CbCln3Δex7
T8839 10616-10617 HYPH denotes /
T8840 10617-10618 CD denotes 8
T8841 10625-10627 VBZ denotes is
T8842 10633-10634 . denotes .
T8843 10634-10730 sentence denotes Wild-type cultures (CbCln3+/+) exhibited limited subunit c immunostaining and autofluorescence.
T8844 10635-10639 JJ denotes Wild
T8845 10640-10644 NN denotes type
T8846 10639-10640 HYPH denotes -
T8847 10645-10653 NNS denotes cultures
T8848 10666-10675 VBD denotes exhibited
T8849 10654-10655 -LRB- denotes (
T8850 10655-10661 NN denotes CbCln3
T8851 10661-10662 SYM denotes +
T8852 10662-10663 HYPH denotes /
T8853 10663-10664 SYM denotes +
T8854 10664-10665 -RRB- denotes )
T8855 10676-10683 JJ denotes limited
T8856 10692-10693 NN denotes c
T8857 10684-10691 NN denotes subunit
T8858 10694-10708 NN denotes immunostaining
T8859 10709-10712 CC denotes and
T8860 10713-10729 NN denotes autofluorescence
T8861 10729-10730 . denotes .
T8862 10730-10802 sentence denotes However, CbCln3Δex7/8/Δex7/8 cells contained numerous subunit c puncta.
T8863 10731-10738 RB denotes However
T8864 10766-10775 VBD denotes contained
T8865 10738-10740 , denotes ,
T8866 10740-10750 NN denotes CbCln3Δex7
T8867 10753-10757 NN denotes Δex7
T8868 10750-10751 HYPH denotes /
T8869 10751-10752 CD denotes 8
T8870 10752-10753 HYPH denotes /
T8871 10760-10765 NNS denotes cells
T8872 10757-10758 HYPH denotes /
T8873 10758-10759 CD denotes 8
T8874 10776-10784 JJ denotes numerous
T8875 10795-10801 NNS denotes puncta
T8876 10785-10792 NN denotes subunit
T8877 10793-10794 NN denotes c
T8878 10801-10802 . denotes .
T8879 10802-10949 sentence denotes Autofluorescence (7 days AF) was also significantly elevated (right panels), although limited overlap with subunit c puncta was observed (arrows).
T8880 10803-10819 NN denotes Autofluorescence
T8881 10832-10835 VBD denotes was
T8882 10820-10821 -LRB- denotes (
T8883 10828-10830 NN denotes AF
T8884 10821-10822 CD denotes 7
T8885 10823-10827 NNS denotes days
T8886 10830-10831 -RRB- denotes )
T8887 10836-10840 RB denotes also
T8888 10841-10854 RB denotes significantly
T8889 10855-10863 VBN denotes elevated
T8890 10864-10865 -LRB- denotes (
T8891 10871-10877 NNS denotes panels
T8892 10865-10870 JJ denotes right
T8893 10877-10878 -RRB- denotes )
T8894 10878-10880 , denotes ,
T8895 10880-10888 IN denotes although
T8896 10931-10939 VBN denotes observed
T8897 10889-10896 JJ denotes limited
T8898 10897-10904 NN denotes overlap
T8899 10905-10909 IN denotes with
T8900 10910-10917 NN denotes subunit
T8901 10918-10919 NN denotes c
T8902 10920-10926 NNS denotes puncta
T8903 10927-10930 VBD denotes was
T8904 10940-10941 -LRB- denotes (
T8905 10941-10947 NNS denotes arrows
T8906 10947-10948 -RRB- denotes )
T8907 10948-10949 . denotes .
T8908 10949-10969 sentence denotes 40 × magnification.
T8909 10950-10952 CD denotes 40
T8910 10955-10968 NN denotes magnification
T8911 10953-10954 SYM denotes ×
T8912 10968-10969 . denotes .
T8913 10969-11072 sentence denotes b. Immunoblot analysis of subunit c protein at sub-confluency or 7-day confluency incubation is shown.
T8914 10970-10971 LS denotes b
T8915 11066-11071 VBN denotes shown
T8916 10971-10972 . denotes .
T8917 10973-10983 NN denotes Immunoblot
T8918 10984-10992 NN denotes analysis
T8919 10993-10995 IN denotes of
T8920 10996-11003 NN denotes subunit
T8921 11004-11005 NN denotes c
T8922 11006-11013 NN denotes protein
T8923 11014-11016 IN denotes at
T8924 11017-11031 NN denotes sub-confluency
T8925 11052-11062 NN denotes incubation
T8926 11032-11034 CC denotes or
T8927 11035-11036 CD denotes 7
T8928 11037-11040 NN denotes day
T8929 11036-11037 HYPH denotes -
T8930 11041-11051 NN denotes confluency
T8931 11063-11065 VBZ denotes is
T8932 11071-11072 . denotes .
T8933 11072-11247 sentence denotes Total protein extracts from sub-confluency wild-type (+/+) and homozygous mutant (Δex7/8/Δex7/8) cultures contained approximately equal levels of subunit c protein (α-sub c).
T8934 11073-11078 JJ denotes Total
T8935 11087-11095 NNS denotes extracts
T8936 11079-11086 NN denotes protein
T8937 11179-11188 VBD denotes contained
T8938 11096-11100 IN denotes from
T8939 11101-11115 NN denotes sub-confluency
T8940 11170-11178 NNS denotes cultures
T8941 11116-11120 JJ denotes wild
T8942 11121-11125 NN denotes type
T8943 11120-11121 HYPH denotes -
T8944 11126-11127 -LRB- denotes (
T8945 11127-11128 SYM denotes +
T8946 11129-11130 SYM denotes +
T8947 11128-11129 HYPH denotes /
T8948 11130-11131 -RRB- denotes )
T8949 11132-11135 CC denotes and
T8950 11136-11146 JJ denotes homozygous
T8951 11147-11153 NN denotes mutant
T8952 11154-11155 -LRB- denotes (
T8953 11155-11159 NN denotes Δex7
T8954 11159-11160 HYPH denotes /
T8955 11160-11161 CD denotes 8
T8956 11161-11162 HYPH denotes /
T8957 11162-11166 NN denotes Δex7
T8958 11166-11167 HYPH denotes /
T8959 11167-11168 CD denotes 8
T8960 11168-11169 -RRB- denotes )
T8961 11189-11202 RB denotes approximately
T8962 11203-11208 JJ denotes equal
T8963 11209-11215 NNS denotes levels
T8964 11216-11218 IN denotes of
T8965 11219-11226 NN denotes subunit
T8966 11227-11228 NN denotes c
T8967 11229-11236 NN denotes protein
T8968 11237-11238 -LRB- denotes (
T8969 11238-11239 NN denotes α
T8970 11244-11245 NN denotes c
T8971 11239-11240 HYPH denotes -
T8972 11240-11243 JJ denotes sub
T8973 11245-11246 -RRB- denotes )
T8974 11246-11247 . denotes .
T8975 11247-11410 sentence denotes 7-day confluency extract from homozygous CbCln3Δex7/8 cells (Δex7/8/Δex7/8) had elevated levels of subunit c protein (~1.5X), relative to wild-type extract (+/+).
T8976 11248-11249 CD denotes 7
T8977 11250-11253 NN denotes day
T8978 11249-11250 HYPH denotes -
T8979 11254-11264 NN denotes confluency
T8980 11265-11272 NN denotes extract
T8981 11324-11327 VBD denotes had
T8982 11273-11277 IN denotes from
T8983 11278-11288 JJ denotes homozygous
T8984 11302-11307 NNS denotes cells
T8985 11289-11299 NN denotes CbCln3Δex7
T8986 11299-11300 HYPH denotes /
T8987 11300-11301 CD denotes 8
T8988 11308-11309 -LRB- denotes (
T8989 11309-11313 NN denotes Δex7
T8990 11313-11314 HYPH denotes /
T8991 11314-11315 CD denotes 8
T8992 11315-11316 HYPH denotes /
T8993 11316-11320 NN denotes Δex7
T8994 11320-11321 HYPH denotes /
T8995 11321-11322 CD denotes 8
T8996 11322-11323 -RRB- denotes )
T8997 11328-11336 VBN denotes elevated
T8998 11337-11343 NNS denotes levels
T8999 11344-11346 IN denotes of
T9000 11347-11354 NN denotes subunit
T9001 11355-11356 NN denotes c
T9002 11357-11364 NN denotes protein
T9003 11365-11366 -LRB- denotes (
T9004 11366-11367 SYM denotes ~
T9005 11367-11370 CD denotes 1.5
T9006 11370-11371 SYM denotes X
T9007 11371-11372 -RRB- denotes )
T9008 11372-11374 , denotes ,
T9009 11374-11382 JJ denotes relative
T9010 11383-11385 IN denotes to
T9011 11386-11390 JJ denotes wild
T9012 11391-11395 NN denotes type
T9013 11390-11391 HYPH denotes -
T9014 11396-11403 NN denotes extract
T9015 11404-11405 -LRB- denotes (
T9016 11405-11406 SYM denotes +
T9017 11407-11408 SYM denotes +
T9018 11406-11407 HYPH denotes /
T9019 11408-11409 -RRB- denotes )
T9020 11409-11410 . denotes .
T9021 11410-11486 sentence denotes Protein levels were normalized to cytochrome c oxidase subunit IV (α-cox4).
T9022 11411-11418 NN denotes Protein
T9023 11419-11425 NNS denotes levels
T9024 11431-11441 VBN denotes normalized
T9025 11426-11430 VBD denotes were
T9026 11442-11444 IN denotes to
T9027 11445-11455 NN denotes cytochrome
T9028 11456-11457 NN denotes c
T9029 11458-11465 NN denotes oxidase
T9030 11466-11473 NN denotes subunit
T9031 11474-11476 CD denotes IV
T9032 11477-11478 -LRB- denotes (
T9033 11478-11479 NN denotes α
T9034 11480-11484 NN denotes cox4
T9035 11479-11480 HYPH denotes -
T9036 11484-11485 -RRB- denotes )
T9037 11485-11486 . denotes .
T9038 11486-11581 sentence denotes c. TEM analysis of inclusions in 7-day confluency-aged homozygous CbCln3Δex7/8 cells is shown.
T9039 11487-11488 LS denotes c
T9040 11575-11580 VBN denotes shown
T9041 11488-11489 . denotes .
T9042 11490-11493 NN denotes TEM
T9043 11494-11502 NN denotes analysis
T9044 11503-11505 IN denotes of
T9045 11506-11516 NNS denotes inclusions
T9046 11517-11519 IN denotes in
T9047 11520-11521 CD denotes 7
T9048 11522-11525 NN denotes day
T9049 11521-11522 HYPH denotes -
T9050 11526-11536 NN denotes confluency
T9051 11537-11541 VBN denotes aged
T9052 11536-11537 HYPH denotes -
T9053 11566-11571 NNS denotes cells
T9054 11542-11552 JJ denotes homozygous
T9055 11553-11563 NN denotes CbCln3Δex7
T9056 11563-11564 HYPH denotes /
T9057 11564-11565 CD denotes 8
T9058 11572-11574 VBZ denotes is
T9059 11580-11581 . denotes .
T9060 11581-11771 sentence denotes A large autophagosome contained by double membrane (arrows) is filled with degenerating mitochondria (Md), electron dense cores (left and right of *) and other smaller vesicular structures.
T9061 11582-11583 DT denotes A
T9062 11590-11603 NN denotes autophagosome
T9063 11584-11589 JJ denotes large
T9064 11645-11651 VBN denotes filled
T9065 11604-11613 VBN denotes contained
T9066 11614-11616 IN denotes by
T9067 11617-11623 JJ denotes double
T9068 11624-11632 NN denotes membrane
T9069 11633-11634 -LRB- denotes (
T9070 11634-11640 NNS denotes arrows
T9071 11640-11641 -RRB- denotes )
T9072 11642-11644 VBZ denotes is
T9073 11652-11656 IN denotes with
T9074 11657-11669 VBG denotes degenerating
T9075 11670-11682 NNS denotes mitochondria
T9076 11683-11684 -LRB- denotes (
T9077 11684-11686 NN denotes Md
T9078 11686-11687 -RRB- denotes )
T9079 11687-11689 , denotes ,
T9080 11689-11697 NN denotes electron
T9081 11704-11709 NNS denotes cores
T9082 11698-11703 JJ denotes dense
T9083 11710-11711 -LRB- denotes (
T9084 11711-11715 JJ denotes left
T9085 11716-11719 CC denotes and
T9086 11720-11725 JJ denotes right
T9087 11726-11728 IN denotes of
T9088 11729-11730 SYM denotes *
T9089 11730-11731 -RRB- denotes )
T9090 11732-11735 CC denotes and
T9091 11736-11741 JJ denotes other
T9092 11760-11770 NNS denotes structures
T9093 11742-11749 JJR denotes smaller
T9094 11750-11759 JJ denotes vesicular
T9095 11770-11771 . denotes .
T9096 11771-11857 sentence denotes A large electron-dense inclusion, with a lipofuscin (Ln) appearance, is also present.
T9097 11772-11773 DT denotes A
T9098 11795-11804 NN denotes inclusion
T9099 11774-11779 JJ denotes large
T9100 11780-11788 NN denotes electron
T9101 11789-11794 JJ denotes dense
T9102 11788-11789 HYPH denotes -
T9103 11841-11843 VBZ denotes is
T9104 11804-11806 , denotes ,
T9105 11806-11810 IN denotes with
T9106 11811-11812 DT denotes a
T9107 11829-11839 NN denotes appearance
T9108 11813-11823 NN denotes lipofuscin
T9109 11824-11825 -LRB- denotes (
T9110 11825-11827 NN denotes Ln
T9111 11827-11828 -RRB- denotes )
T9112 11839-11841 , denotes ,
T9113 11844-11848 RB denotes also
T9114 11849-11856 JJ denotes present
T9115 11856-11857 . denotes .
T9116 11857-11874 sentence denotes M, mitochondria.
T9117 11858-11859 NN denotes M
T9118 11859-11861 , denotes ,
T9119 11861-11873 NNS denotes mitochondria
T9120 11873-11874 . denotes .
T9121 11874-11898 sentence denotes 10,000 × magnification.
T9122 11875-11881 CD denotes 10,000
T9123 11884-11897 NN denotes magnification
T9124 11882-11883 SYM denotes ×
T9125 11897-11898 . denotes .
T9254 13625-13634 NN denotes Cathepsin
T9255 13635-13636 NN denotes D
T9256 13637-13649 NN denotes localization
T9257 13650-13653 CC denotes and
T9258 13654-13664 NN denotes processing
T9259 13665-13667 IN denotes in
T9260 13668-13672 JJ denotes wild
T9261 13673-13677 NN denotes type
T9262 13672-13673 HYPH denotes -
T9263 13706-13711 NNS denotes cells
T9264 13678-13681 CC denotes and
T9265 13682-13692 JJ denotes homozygous
T9266 13693-13703 NN denotes CbCln3Δex7
T9267 13703-13704 HYPH denotes /
T9268 13704-13705 CD denotes 8
T9269 13711-13895 sentence denotes a. Immunostaining of wild-type and homozygous CbCln3Δex7/8 precursor cells with anti-cathepsin D antibody, recognizing unprocessed and processed forms of cathepsin D protein is shown.
T9270 13712-13713 LS denotes a
T9271 13889-13894 VBN denotes shown
T9272 13713-13714 . denotes .
T9273 13715-13729 NN denotes Immunostaining
T9274 13730-13732 IN denotes of
T9275 13733-13737 JJ denotes wild
T9276 13738-13742 NN denotes type
T9277 13737-13738 HYPH denotes -
T9278 13781-13786 NNS denotes cells
T9279 13743-13746 CC denotes and
T9280 13747-13757 JJ denotes homozygous
T9281 13758-13768 NN denotes CbCln3Δex7
T9282 13768-13769 HYPH denotes /
T9283 13769-13770 CD denotes 8
T9284 13771-13780 NN denotes precursor
T9285 13787-13791 IN denotes with
T9286 13792-13806 JJ denotes anti-cathepsin
T9287 13807-13808 NN denotes D
T9288 13809-13817 NN denotes antibody
T9289 13817-13819 , denotes ,
T9290 13819-13830 VBG denotes recognizing
T9291 13831-13842 JJ denotes unprocessed
T9292 13857-13862 NNS denotes forms
T9293 13843-13846 CC denotes and
T9294 13847-13856 VBN denotes processed
T9295 13863-13865 IN denotes of
T9296 13866-13875 NN denotes cathepsin
T9297 13876-13877 NN denotes D
T9298 13878-13885 NN denotes protein
T9299 13886-13888 VBZ denotes is
T9300 13894-13895 . denotes .
T9301 13895-13981 sentence denotes CbCln3+/+ cells (left panel) exhibited a perinuclear and cytoplasmic punctate signal.
T9302 13896-13902 NN denotes CbCln3
T9303 13906-13911 NNS denotes cells
T9304 13902-13903 SYM denotes +
T9305 13903-13904 HYPH denotes /
T9306 13904-13905 SYM denotes +
T9307 13925-13934 VBD denotes exhibited
T9308 13912-13913 -LRB- denotes (
T9309 13918-13923 NN denotes panel
T9310 13913-13917 JJ denotes left
T9311 13923-13924 -RRB- denotes )
T9312 13935-13936 DT denotes a
T9313 13974-13980 NN denotes signal
T9314 13937-13948 JJ denotes perinuclear
T9315 13949-13952 CC denotes and
T9316 13953-13964 JJ denotes cytoplasmic
T9317 13965-13973 JJ denotes punctate
T9318 13980-13981 . denotes .
T9319 13981-14153 sentence denotes Cathepsin D signal in homozygous CbCln3Δex7/8 cells (right panel) was more often perinuclear, with less cytoplasmic punctate signal, compared to wild-type CbCln3+/+ cells.
T9320 13982-13991 NN denotes Cathepsin
T9321 13992-13993 NN denotes D
T9322 13994-14000 NN denotes signal
T9323 14048-14051 VBD denotes was
T9324 14001-14003 IN denotes in
T9325 14004-14014 JJ denotes homozygous
T9326 14028-14033 NNS denotes cells
T9327 14015-14025 NN denotes CbCln3Δex7
T9328 14025-14026 HYPH denotes /
T9329 14026-14027 CD denotes 8
T9330 14034-14035 -LRB- denotes (
T9331 14041-14046 NN denotes panel
T9332 14035-14040 JJ denotes right
T9333 14046-14047 -RRB- denotes )
T9334 14052-14056 RBR denotes more
T9335 14057-14062 RB denotes often
T9336 14063-14074 JJ denotes perinuclear
T9337 14074-14076 , denotes ,
T9338 14076-14080 IN denotes with
T9339 14081-14085 RBR denotes less
T9340 14107-14113 NN denotes signal
T9341 14086-14097 JJ denotes cytoplasmic
T9342 14098-14106 NN denotes punctate
T9343 14113-14115 , denotes ,
T9344 14115-14123 VBN denotes compared
T9345 14124-14126 IN denotes to
T9346 14127-14131 JJ denotes wild
T9347 14132-14136 NN denotes type
T9348 14131-14132 HYPH denotes -
T9349 14147-14152 NNS denotes cells
T9350 14137-14143 NN denotes CbCln3
T9351 14143-14144 SYM denotes +
T9352 14144-14145 HYPH denotes /
T9353 14145-14146 SYM denotes +
T9354 14152-14153 . denotes .
T9355 14153-14173 sentence denotes 40 × magnification.
T9356 14154-14156 CD denotes 40
T9357 14159-14172 NN denotes magnification
T9358 14157-14158 SYM denotes ×
T9359 14172-14173 . denotes .
T9360 14173-14318 sentence denotes b. α-Cathepsin D-probed immunoblots of total wild-type versus homozygous Cln3Δex7/8 knock-in tissue or CbCln3Δex7/8 cellular extracts are shown.
T9361 14174-14175 LS denotes b
T9362 14312-14317 VBN denotes shown
T9363 14175-14176 . denotes .
T9364 14177-14178 NN denotes α
T9365 14189-14190 NN denotes D
T9366 14178-14179 HYPH denotes -
T9367 14179-14188 NN denotes Cathepsin
T9368 14191-14197 VBN denotes probed
T9369 14190-14191 HYPH denotes -
T9370 14198-14209 NNS denotes immunoblots
T9371 14210-14212 IN denotes of
T9372 14213-14218 JJ denotes total
T9373 14267-14273 NN denotes tissue
T9374 14219-14223 JJ denotes wild
T9375 14224-14228 NN denotes type
T9376 14223-14224 HYPH denotes -
T9377 14229-14235 CC denotes versus
T9378 14236-14246 JJ denotes homozygous
T9379 14247-14255 NN denotes Cln3Δex7
T9380 14258-14263 VB denotes knock
T9381 14255-14256 HYPH denotes /
T9382 14256-14257 CD denotes 8
T9383 14263-14264 HYPH denotes -
T9384 14264-14266 RP denotes in
T9385 14274-14276 CC denotes or
T9386 14277-14287 NN denotes CbCln3Δex7
T9387 14299-14307 NNS denotes extracts
T9388 14287-14288 HYPH denotes /
T9389 14288-14289 CD denotes 8
T9390 14290-14298 JJ denotes cellular
T9391 14308-14311 VBP denotes are
T9392 14317-14318 . denotes .
T9393 14318-14531 sentence denotes The ~45 kDa cathepsin D band, representing precursor, was the predominant band in wild-type (wt) tissue and cellular extracts, with lower levels of mature enzyme (single chain, ~43 kDa, and heavy chain, ~31 kDa).
T9394 14319-14322 DT denotes The
T9395 14343-14347 NN denotes band
T9396 14323-14324 SYM denotes ~
T9397 14324-14326 CD denotes 45
T9398 14327-14330 NN denotes kDa
T9399 14331-14340 NN denotes cathepsin
T9400 14341-14342 NN denotes D
T9401 14373-14376 VBD denotes was
T9402 14347-14349 , denotes ,
T9403 14349-14361 VBG denotes representing
T9404 14362-14371 NN denotes precursor
T9405 14371-14373 , denotes ,
T9406 14377-14380 DT denotes the
T9407 14393-14397 NN denotes band
T9408 14381-14392 JJ denotes predominant
T9409 14398-14400 IN denotes in
T9410 14401-14405 JJ denotes wild
T9411 14406-14410 NN denotes type
T9412 14405-14406 HYPH denotes -
T9413 14411-14412 -LRB- denotes (
T9414 14412-14414 NN denotes wt
T9415 14414-14415 -RRB- denotes )
T9416 14416-14422 NN denotes tissue
T9417 14423-14426 CC denotes and
T9418 14427-14435 JJ denotes cellular
T9419 14436-14444 NNS denotes extracts
T9420 14444-14446 , denotes ,
T9421 14446-14450 IN denotes with
T9422 14451-14456 JJR denotes lower
T9423 14457-14463 NNS denotes levels
T9424 14464-14466 IN denotes of
T9425 14467-14473 JJ denotes mature
T9426 14474-14480 NN denotes enzyme
T9427 14481-14482 -LRB- denotes (
T9428 14500-14503 NN denotes kDa
T9429 14482-14488 JJ denotes single
T9430 14489-14494 NN denotes chain
T9431 14494-14496 , denotes ,
T9432 14496-14497 SYM denotes ~
T9433 14497-14499 CD denotes 43
T9434 14503-14505 , denotes ,
T9435 14505-14508 CC denotes and
T9436 14509-14514 JJ denotes heavy
T9437 14515-14520 NN denotes chain
T9438 14526-14529 NN denotes kDa
T9439 14520-14522 , denotes ,
T9440 14522-14523 SYM denotes ~
T9441 14523-14525 CD denotes 31
T9442 14529-14530 -RRB- denotes )
T9443 14530-14531 . denotes .
T9444 14531-14748 sentence denotes Conversely, homozygous Cln3Δex7/8 and CbCln3Δex7/8 mutant (m) extracts exhibited reduced levels of precursor and heavy chain of the double-chain form of the enzyme, with elevated levels of single-chain mature enzyme.
T9445 14532-14542 RB denotes Conversely
T9446 14603-14612 VBD denotes exhibited
T9447 14542-14544 , denotes ,
T9448 14544-14554 JJ denotes homozygous
T9449 14594-14602 NNS denotes extracts
T9450 14555-14563 NN denotes Cln3Δex7
T9451 14563-14564 HYPH denotes /
T9452 14564-14565 CD denotes 8
T9453 14566-14569 CC denotes and
T9454 14570-14580 NN denotes CbCln3Δex7
T9455 14580-14581 HYPH denotes /
T9456 14581-14582 CD denotes 8
T9457 14583-14589 NN denotes mutant
T9458 14590-14591 -LRB- denotes (
T9459 14591-14592 NN denotes m
T9460 14592-14593 -RRB- denotes )
T9461 14613-14620 VBN denotes reduced
T9462 14621-14627 NNS denotes levels
T9463 14628-14630 IN denotes of
T9464 14631-14640 NN denotes precursor
T9465 14641-14644 CC denotes and
T9466 14645-14650 JJ denotes heavy
T9467 14651-14656 NN denotes chain
T9468 14657-14659 IN denotes of
T9469 14660-14663 DT denotes the
T9470 14677-14681 NN denotes form
T9471 14664-14670 JJ denotes double
T9472 14671-14676 NN denotes chain
T9473 14670-14671 HYPH denotes -
T9474 14682-14684 IN denotes of
T9475 14685-14688 DT denotes the
T9476 14689-14695 NN denotes enzyme
T9477 14695-14697 , denotes ,
T9478 14697-14701 IN denotes with
T9479 14702-14710 JJ denotes elevated
T9480 14711-14717 NNS denotes levels
T9481 14718-14720 IN denotes of
T9482 14721-14727 JJ denotes single
T9483 14728-14733 NN denotes chain
T9484 14727-14728 HYPH denotes -
T9485 14741-14747 NN denotes enzyme
T9486 14734-14740 JJ denotes mature
T9487 14747-14748 . denotes .
T9581 16951-16962 NN denotes Lysotracker
T9582 16974-16982 NN denotes labeling
T9583 16963-16966 CC denotes and
T9584 16967-16971 NN denotes Lamp
T9585 16972-16973 CD denotes 2
T9586 17150-17155 VBN denotes shown
T9587 16983-16985 IN denotes of
T9588 16986-16990 JJ denotes wild
T9589 16991-16995 NN denotes type
T9590 16990-16991 HYPH denotes -
T9591 17024-17033 NNS denotes lysosomes
T9592 16996-16999 CC denotes and
T9593 17000-17010 JJ denotes homozygous
T9594 17011-17021 NN denotes CbCln3Δex7
T9595 17021-17022 HYPH denotes /
T9596 17022-17023 CD denotes 8
T9597 17034-17043 JJ denotes Lysosomal
T9598 17044-17052 NN denotes labeling
T9599 17053-17055 IN denotes of
T9600 17056-17060 JJ denotes wild
T9601 17061-17065 NN denotes type
T9602 17060-17061 HYPH denotes -
T9603 17104-17109 NNS denotes cells
T9604 17066-17069 CC denotes and
T9605 17070-17080 JJ denotes homozygous
T9606 17081-17091 NN denotes CbCln3Δex7
T9607 17091-17092 HYPH denotes /
T9608 17092-17093 CD denotes 8
T9609 17094-17103 NN denotes precursor
T9610 17110-17114 IN denotes with
T9611 17115-17126 NN denotes lysotracker
T9612 17138-17146 NN denotes antibody
T9613 17127-17130 CC denotes and
T9614 17131-17135 NN denotes Lamp
T9615 17136-17137 CD denotes 2
T9616 17147-17149 VBZ denotes is
T9617 17155-17156 . denotes .
T9618 17156-17305 sentence denotes Lysotracker dye (top panels) labeled large, perinuclear-clustered lysosomes and scattered lysosomes in the periphery of wild-type cells (CbCln3+/+).
T9619 17157-17168 NN denotes Lysotracker
T9620 17169-17172 NN denotes dye
T9621 17186-17193 VBN denotes labeled
T9622 17173-17174 -LRB- denotes (
T9623 17178-17184 NNS denotes panels
T9624 17174-17177 JJ denotes top
T9625 17184-17185 -RRB- denotes )
T9626 17194-17199 JJ denotes large
T9627 17223-17232 NNS denotes lysosomes
T9628 17199-17201 , denotes ,
T9629 17201-17212 JJ denotes perinuclear
T9630 17213-17222 VBN denotes clustered
T9631 17212-17213 HYPH denotes -
T9632 17233-17236 CC denotes and
T9633 17237-17246 VBN denotes scattered
T9634 17247-17256 NNS denotes lysosomes
T9635 17257-17259 IN denotes in
T9636 17260-17263 DT denotes the
T9637 17264-17273 NN denotes periphery
T9638 17274-17276 IN denotes of
T9639 17277-17281 JJ denotes wild
T9640 17282-17286 NN denotes type
T9641 17281-17282 HYPH denotes -
T9642 17287-17292 NNS denotes cells
T9643 17293-17294 -LRB- denotes (
T9644 17294-17300 NN denotes CbCln3
T9645 17300-17301 SYM denotes +
T9646 17301-17302 HYPH denotes /
T9647 17302-17303 SYM denotes +
T9648 17303-17304 -RRB- denotes )
T9649 17304-17305 . denotes .
T9650 17305-17470 sentence denotes Lysotracker stain was dramatically reduced in homozygous mutant cells (CbCln3Δex7/8/Δex7/8), with smaller labeled vesicles and less apparent perinuclear clustering.
T9651 17306-17317 NN denotes Lysotracker
T9652 17318-17323 NN denotes stain
T9653 17324-17327 VBD denotes was
T9654 17328-17340 RB denotes dramatically
T9655 17341-17348 JJ denotes reduced
T9656 17349-17351 IN denotes in
T9657 17352-17362 JJ denotes homozygous
T9658 17370-17375 NNS denotes cells
T9659 17363-17369 NN denotes mutant
T9660 17376-17377 -LRB- denotes (
T9661 17377-17387 NN denotes CbCln3Δex7
T9662 17387-17388 HYPH denotes /
T9663 17388-17389 CD denotes 8
T9664 17389-17390 HYPH denotes /
T9665 17390-17394 NN denotes Δex7
T9666 17394-17395 HYPH denotes /
T9667 17395-17396 CD denotes 8
T9668 17396-17397 -RRB- denotes )
T9669 17397-17399 , denotes ,
T9670 17399-17403 IN denotes with
T9671 17404-17411 JJR denotes smaller
T9672 17420-17428 NNS denotes vesicles
T9673 17412-17419 VBN denotes labeled
T9674 17429-17432 CC denotes and
T9675 17433-17437 RBR denotes less
T9676 17438-17446 JJ denotes apparent
T9677 17459-17469 NN denotes clustering
T9678 17447-17458 JJ denotes perinuclear
T9679 17469-17470 . denotes .
T9680 17470-17700 sentence denotes Lamp 2 (bottom panels) immunostaining also showed reduced signal intensity with less perinuclear clustering in homozygous CbCln3Δex7/8 cells, although the effect was somewhat less dramatic than that observed with Lysotracker dye.
T9681 17471-17475 NN denotes Lamp
T9682 17494-17508 NN denotes immunostaining
T9683 17476-17477 CD denotes 2
T9684 17478-17479 -LRB- denotes (
T9685 17486-17492 NNS denotes panels
T9686 17479-17485 JJ denotes bottom
T9687 17492-17493 -RRB- denotes )
T9688 17514-17520 VBD denotes showed
T9689 17509-17513 RB denotes also
T9690 17521-17528 VBN denotes reduced
T9691 17536-17545 NN denotes intensity
T9692 17529-17535 NN denotes signal
T9693 17546-17550 IN denotes with
T9694 17551-17555 RBR denotes less
T9695 17568-17578 NN denotes clustering
T9696 17556-17567 JJ denotes perinuclear
T9697 17579-17581 IN denotes in
T9698 17582-17592 JJ denotes homozygous
T9699 17606-17611 NNS denotes cells
T9700 17593-17603 NN denotes CbCln3Δex7
T9701 17603-17604 HYPH denotes /
T9702 17604-17605 CD denotes 8
T9703 17611-17613 , denotes ,
T9704 17613-17621 IN denotes although
T9705 17633-17636 VBD denotes was
T9706 17622-17625 DT denotes the
T9707 17626-17632 NN denotes effect
T9708 17637-17645 RB denotes somewhat
T9709 17646-17650 RBR denotes less
T9710 17651-17659 JJ denotes dramatic
T9711 17660-17664 IN denotes than
T9712 17665-17669 DT denotes that
T9713 17670-17678 VBN denotes observed
T9714 17679-17683 IN denotes with
T9715 17684-17695 NN denotes Lysotracker
T9716 17696-17699 NN denotes dye
T9717 17699-17700 . denotes .
T9718 17700-17802 sentence denotes Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings.
T9719 17701-17705 JJ denotes Wild
T9720 17706-17710 NN denotes type
T9721 17705-17706 HYPH denotes -
T9722 17748-17754 NNS denotes images
T9723 17711-17714 CC denotes and
T9724 17715-17725 JJ denotes homozygous
T9725 17726-17736 NN denotes CbCln3Δex7
T9726 17736-17737 HYPH denotes /
T9727 17737-17738 CD denotes 8
T9728 17739-17747 JJ denotes confocal
T9729 17760-17768 VBN denotes captured
T9730 17755-17759 VBD denotes were
T9731 17769-17773 IN denotes with
T9732 17774-17783 JJ denotes identical
T9733 17793-17801 NNS denotes settings
T9734 17784-17792 NN denotes exposure
T9735 17801-17802 . denotes .
T9736 17802-17822 sentence denotes 60 × magnification.
T9737 17803-17805 CD denotes 60
T9738 17808-17821 NN denotes magnification
T9739 17806-17807 SYM denotes ×
T9740 17821-17822 . denotes .
T9816 18429-18440 NN denotes Endocytosis
T9817 18596-18601 VBN denotes shown
T9818 18441-18443 IN denotes in
T9819 18444-18448 JJ denotes wild
T9820 18449-18453 NN denotes type
T9821 18448-18449 HYPH denotes -
T9822 18496-18501 NNS denotes cells
T9823 18453-18455 , denotes ,
T9824 18455-18467 JJ denotes heterozygous
T9825 18468-18471 CC denotes and
T9826 18472-18482 JJ denotes homozygous
T9827 18483-18493 NN denotes CbCln3Δex7
T9828 18493-18494 HYPH denotes /
T9829 18494-18495 CD denotes 8
T9830 18502-18509 NN denotes Dextran
T9831 18510-18514 NN denotes FITC
T9832 18509-18510 HYPH denotes -
T9833 18515-18521 NN denotes uptake
T9834 18522-18524 IN denotes in
T9835 18525-18529 JJ denotes wild
T9836 18530-18534 NN denotes type
T9837 18529-18530 HYPH denotes -
T9838 18587-18592 NNS denotes cells
T9839 18534-18536 , denotes ,
T9840 18536-18548 JJ denotes heterozygous
T9841 18549-18552 CC denotes and
T9842 18553-18563 JJ denotes homozygous
T9843 18564-18574 NN denotes CbCln3Δex7
T9844 18574-18575 HYPH denotes /
T9845 18575-18576 CD denotes 8
T9846 18577-18586 NN denotes precursor
T9847 18593-18595 VBZ denotes is
T9848 18601-18602 . denotes .
T9849 18602-18834 sentence denotes In wild-type (CbCln3+/+, left panel) and heterozygous (CbCln3+/Δex7/8, middle panel) cells, dextran-FITC label was observed in a perinuclear-clustered vesicular pattern with scattered labeled vesicles also present in the periphery.
T9850 18603-18605 IN denotes In
T9851 18718-18726 VBN denotes observed
T9852 18606-18610 JJ denotes wild
T9853 18611-18615 NN denotes type
T9854 18610-18611 HYPH denotes -
T9855 18688-18693 NNS denotes cells
T9856 18616-18617 -LRB- denotes (
T9857 18617-18623 NN denotes CbCln3
T9858 18623-18624 SYM denotes +
T9859 18624-18625 HYPH denotes /
T9860 18625-18626 SYM denotes +
T9861 18626-18628 , denotes ,
T9862 18633-18638 NN denotes panel
T9863 18628-18632 JJ denotes left
T9864 18638-18639 -RRB- denotes )
T9865 18640-18643 CC denotes and
T9866 18644-18656 JJ denotes heterozygous
T9867 18657-18658 -LRB- denotes (
T9868 18681-18686 NN denotes panel
T9869 18658-18664 NN denotes CbCln3
T9870 18666-18670 NN denotes Δex7
T9871 18664-18665 SYM denotes +
T9872 18665-18666 HYPH denotes /
T9873 18670-18671 HYPH denotes /
T9874 18671-18672 CD denotes 8
T9875 18672-18674 , denotes ,
T9876 18674-18680 JJ denotes middle
T9877 18686-18687 -RRB- denotes )
T9878 18693-18695 , denotes ,
T9879 18695-18702 NN denotes dextran
T9880 18703-18707 NN denotes FITC
T9881 18702-18703 HYPH denotes -
T9882 18708-18713 NN denotes label
T9883 18714-18717 VBD denotes was
T9884 18727-18729 IN denotes in
T9885 18730-18731 DT denotes a
T9886 18764-18771 NN denotes pattern
T9887 18732-18743 JJ denotes perinuclear
T9888 18744-18753 VBN denotes clustered
T9889 18743-18744 HYPH denotes -
T9890 18754-18763 JJ denotes vesicular
T9891 18772-18776 IN denotes with
T9892 18777-18786 VBN denotes scattered
T9893 18795-18803 NNS denotes vesicles
T9894 18787-18794 VBN denotes labeled
T9895 18804-18808 RB denotes also
T9896 18809-18816 JJ denotes present
T9897 18817-18819 IN denotes in
T9898 18820-18823 DT denotes the
T9899 18824-18833 NN denotes periphery
T9900 18833-18834 . denotes .
T9901 18834-19021 sentence denotes In contrast, dextran-FITC label of homozygous mutant (CbCln3Δex7/8/Δex7/8, right panel) cells was reduced overall and exhibited smaller stained vesicles with less perinuclear clustering.
T9902 18835-18837 IN denotes In
T9903 18933-18940 VBN denotes reduced
T9904 18838-18846 NN denotes contrast
T9905 18846-18848 , denotes ,
T9906 18848-18855 NN denotes dextran
T9907 18856-18860 NN denotes FITC
T9908 18855-18856 HYPH denotes -
T9909 18861-18866 NN denotes label
T9910 18867-18869 IN denotes of
T9911 18870-18880 JJ denotes homozygous
T9912 18881-18887 NN denotes mutant
T9913 18923-18928 NNS denotes cells
T9914 18888-18889 -LRB- denotes (
T9915 18889-18899 NN denotes CbCln3Δex7
T9916 18902-18906 NN denotes Δex7
T9917 18899-18900 HYPH denotes /
T9918 18900-18901 CD denotes 8
T9919 18901-18902 HYPH denotes /
T9920 18906-18907 HYPH denotes /
T9921 18907-18908 CD denotes 8
T9922 18908-18910 , denotes ,
T9923 18916-18921 NN denotes panel
T9924 18910-18915 JJ denotes right
T9925 18921-18922 -RRB- denotes )
T9926 18929-18932 VBD denotes was
T9927 18941-18948 RB denotes overall
T9928 18949-18952 CC denotes and
T9929 18953-18962 VBD denotes exhibited
T9930 18963-18970 JJR denotes smaller
T9931 18979-18987 NNS denotes vesicles
T9932 18971-18978 VBN denotes stained
T9933 18988-18992 IN denotes with
T9934 18993-18997 RBR denotes less
T9935 19010-19020 NN denotes clustering
T9936 18998-19009 JJ denotes perinuclear
T9937 19020-19021 . denotes .
T9938 19021-19085 sentence denotes Confocal images were captured with identical exposure settings.
T9939 19022-19030 JJ denotes Confocal
T9940 19031-19037 NNS denotes images
T9941 19043-19051 VBN denotes captured
T9942 19038-19042 VBD denotes were
T9943 19052-19056 IN denotes with
T9944 19057-19066 JJ denotes identical
T9945 19076-19084 NNS denotes settings
T9946 19067-19075 NN denotes exposure
T9947 19084-19085 . denotes .
T9948 19085-19105 sentence denotes 40 × magnification.
T9949 19086-19088 CD denotes 40
T9950 19091-19104 NN denotes magnification
T9951 19089-19090 SYM denotes ×
T9952 19104-19105 . denotes .
T10102 20293-20306 JJ denotes Mitochondrial
T10103 20307-20317 NN denotes morphology
T10104 20318-20321 CC denotes and
T10105 20322-20330 NN denotes function
T10106 20331-20333 IN denotes in
T10107 20334-20338 JJ denotes wild
T10108 20339-20343 NN denotes type
T10109 20338-20339 HYPH denotes -
T10110 20386-20391 NNS denotes cells
T10111 20343-20345 , denotes ,
T10112 20345-20357 JJ denotes heterozygous
T10113 20358-20361 CC denotes and
T10114 20362-20372 JJ denotes homozygous
T10115 20373-20383 NN denotes CbCln3Δex7
T10116 20383-20384 HYPH denotes /
T10117 20384-20385 CD denotes 8
T10118 20391-20500 sentence denotes a. Confocal and TEM micrographs of wild-type and homozygous CbCln3Δex7/8 mitochondrial morphology are shown.
T10119 20392-20393 LS denotes a
T10120 20494-20499 VBN denotes shown
T10121 20393-20394 . denotes .
T10122 20395-20403 JJ denotes Confocal
T10123 20412-20423 NNS denotes micrographs
T10124 20404-20407 CC denotes and
T10125 20408-20411 NN denotes TEM
T10126 20424-20426 IN denotes of
T10127 20427-20431 JJ denotes wild
T10128 20432-20436 NN denotes type
T10129 20431-20432 HYPH denotes -
T10130 20479-20489 NN denotes morphology
T10131 20437-20440 CC denotes and
T10132 20441-20451 JJ denotes homozygous
T10133 20452-20462 NN denotes CbCln3Δex7
T10134 20462-20463 HYPH denotes /
T10135 20463-20464 CD denotes 8
T10136 20465-20478 JJ denotes mitochondrial
T10137 20490-20493 VBP denotes are
T10138 20499-20500 . denotes .
T10139 20500-20736 sentence denotes Immunostaining with the inner mitochondrial membrane marker, grp75 (top panels) highlighted elongated mitochondria in homozygous mutant cells (CbCln3Δex7/8/Δex7/8), relative to wild-type mitochondria (CbCln3+/+) (insets, zoom = 2.75x).
T10140 20501-20515 NN denotes Immunostaining
T10141 20581-20592 VBD denotes highlighted
T10142 20516-20520 IN denotes with
T10143 20521-20524 DT denotes the
T10144 20554-20560 NN denotes marker
T10145 20525-20530 JJ denotes inner
T10146 20531-20544 JJ denotes mitochondrial
T10147 20545-20553 NN denotes membrane
T10148 20560-20562 , denotes ,
T10149 20562-20567 NN denotes grp75
T10150 20568-20569 -LRB- denotes (
T10151 20573-20579 NNS denotes panels
T10152 20569-20572 JJ denotes top
T10153 20579-20580 -RRB- denotes )
T10154 20593-20602 VBN denotes elongated
T10155 20603-20615 NNS denotes mitochondria
T10156 20616-20618 IN denotes in
T10157 20619-20629 JJ denotes homozygous
T10158 20637-20642 NNS denotes cells
T10159 20630-20636 NN denotes mutant
T10160 20643-20644 -LRB- denotes (
T10161 20644-20654 NN denotes CbCln3Δex7
T10162 20657-20661 NN denotes Δex7
T10163 20654-20655 HYPH denotes /
T10164 20655-20656 CD denotes 8
T10165 20656-20657 HYPH denotes /
T10166 20661-20662 HYPH denotes /
T10167 20662-20663 CD denotes 8
T10168 20663-20664 -RRB- denotes )
T10169 20664-20666 , denotes ,
T10170 20666-20674 JJ denotes relative
T10171 20675-20677 IN denotes to
T10172 20678-20682 JJ denotes wild
T10173 20683-20687 NN denotes type
T10174 20682-20683 HYPH denotes -
T10175 20688-20700 NNS denotes mitochondria
T10176 20701-20702 -LRB- denotes (
T10177 20702-20708 NN denotes CbCln3
T10178 20708-20709 SYM denotes +
T10179 20709-20710 HYPH denotes /
T10180 20710-20711 SYM denotes +
T10181 20711-20712 -RRB- denotes )
T10182 20713-20714 -LRB- denotes (
T10183 20714-20720 NNS denotes insets
T10184 20720-20722 , denotes ,
T10185 20722-20726 NN denotes zoom
T10186 20729-20733 CD denotes 2.75
T10187 20727-20728 SYM denotes =
T10188 20733-20734 SYM denotes x
T10189 20734-20735 -RRB- denotes )
T10190 20735-20736 . denotes .
T10191 20736-20807 sentence denotes Mitochondrial distribution was not altered from the wild-type pattern.
T10192 20737-20750 JJ denotes Mitochondrial
T10193 20751-20763 NN denotes distribution
T10194 20772-20779 VBN denotes altered
T10195 20764-20767 VBD denotes was
T10196 20768-20771 RB denotes not
T10197 20780-20784 IN denotes from
T10198 20785-20788 DT denotes the
T10199 20799-20806 NN denotes pattern
T10200 20789-20793 JJ denotes wild
T10201 20794-20798 NN denotes type
T10202 20793-20794 HYPH denotes -
T10203 20806-20807 . denotes .
T10204 20807-20890 sentence denotes Elongated homozygous CbCln3Δex7/8 mitochondria were also observed by TEM analysis.
T10205 20808-20817 VBN denotes Elongated
T10206 20842-20854 NNS denotes mitochondria
T10207 20818-20828 JJ denotes homozygous
T10208 20829-20839 NN denotes CbCln3Δex7
T10209 20839-20840 HYPH denotes /
T10210 20840-20841 CD denotes 8
T10211 20865-20873 VBN denotes observed
T10212 20855-20859 VBD denotes were
T10213 20860-20864 RB denotes also
T10214 20874-20876 IN denotes by
T10215 20877-20880 NN denotes TEM
T10216 20881-20889 NN denotes analysis
T10217 20889-20890 . denotes .
T10218 20890-20910 sentence denotes 60 × magnification.
T10219 20891-20893 CD denotes 60
T10220 20896-20909 NN denotes magnification
T10221 20894-20895 SYM denotes ×
T10222 20909-20910 . denotes .
T10223 20910-21015 sentence denotes b. Cellular ATP levels in wild-type, heterozygous and homozygous CbCln3Δex7/8 precursor cells are shown.
T10224 20911-20912 LS denotes b
T10225 21009-21014 VBN denotes shown
T10226 20912-20913 . denotes .
T10227 20914-20922 JJ denotes Cellular
T10228 20923-20926 NN denotes ATP
T10229 20927-20933 NNS denotes levels
T10230 20934-20936 IN denotes in
T10231 20937-20941 JJ denotes wild
T10232 20942-20946 NN denotes type
T10233 20941-20942 HYPH denotes -
T10234 20999-21004 NNS denotes cells
T10235 20946-20948 , denotes ,
T10236 20948-20960 JJ denotes heterozygous
T10237 20961-20964 CC denotes and
T10238 20965-20975 JJ denotes homozygous
T10239 20976-20986 NN denotes CbCln3Δex7
T10240 20986-20987 HYPH denotes /
T10241 20987-20988 CD denotes 8
T10242 20989-20998 NN denotes precursor
T10243 21005-21008 VBP denotes are
T10244 21014-21015 . denotes .
T10245 21015-21267 sentence denotes Wild-type (open bar) and heterozygous (gray bar) CbCln3Δex7/8 cells contained ~39 μM ATP, while homozygous CbCln3Δex7/8 cells (black bar) contained ~1.3 fold reduced levels of ATP (~30 μM), which was statistically significant in a t-test (p < 0.0001).
T10246 21016-21020 JJ denotes Wild
T10247 21021-21025 NN denotes type
T10248 21020-21021 HYPH denotes -
T10249 21078-21083 NNS denotes cells
T10250 21026-21027 -LRB- denotes (
T10251 21032-21035 NN denotes bar
T10252 21027-21031 JJ denotes open
T10253 21035-21036 -RRB- denotes )
T10254 21037-21040 CC denotes and
T10255 21041-21053 JJ denotes heterozygous
T10256 21054-21055 -LRB- denotes (
T10257 21060-21063 NN denotes bar
T10258 21055-21059 JJ denotes gray
T10259 21063-21064 -RRB- denotes )
T10260 21065-21075 NN denotes CbCln3Δex7
T10261 21075-21076 HYPH denotes /
T10262 21076-21077 CD denotes 8
T10263 21084-21093 VBD denotes contained
T10264 21094-21095 SYM denotes ~
T10265 21095-21097 CD denotes 39
T10266 21098-21100 NN denotes μM
T10267 21101-21104 NN denotes ATP
T10268 21104-21106 , denotes ,
T10269 21106-21111 IN denotes while
T10270 21154-21163 VBD denotes contained
T10271 21112-21122 JJ denotes homozygous
T10272 21136-21141 NNS denotes cells
T10273 21123-21133 NN denotes CbCln3Δex7
T10274 21133-21134 HYPH denotes /
T10275 21134-21135 CD denotes 8
T10276 21142-21143 -LRB- denotes (
T10277 21149-21152 NN denotes bar
T10278 21143-21148 JJ denotes black
T10279 21152-21153 -RRB- denotes )
T10280 21164-21165 SYM denotes ~
T10281 21165-21168 CD denotes 1.3
T10282 21174-21181 VBN denotes reduced
T10283 21169-21173 RB denotes fold
T10284 21182-21188 NNS denotes levels
T10285 21189-21191 IN denotes of
T10286 21192-21195 NN denotes ATP
T10287 21196-21197 -LRB- denotes (
T10288 21197-21198 SYM denotes ~
T10289 21198-21200 CD denotes 30
T10290 21201-21203 NN denotes μM
T10291 21203-21204 -RRB- denotes )
T10292 21204-21206 , denotes ,
T10293 21206-21211 WDT denotes which
T10294 21212-21215 VBD denotes was
T10295 21216-21229 RB denotes statistically
T10296 21230-21241 JJ denotes significant
T10297 21242-21244 IN denotes in
T10298 21245-21246 DT denotes a
T10299 21249-21253 NN denotes test
T10300 21247-21248 NN denotes t
T10301 21248-21249 HYPH denotes -
T10302 21254-21255 -LRB- denotes (
T10303 21259-21265 CD denotes 0.0001
T10304 21255-21256 NN denotes p
T10305 21257-21258 SYM denotes <
T10306 21265-21266 -RRB- denotes )
T10307 21266-21267 . denotes .
T10308 21267-21387 sentence denotes Wild-type and heterozygous CbCln3Δex7/8 cellular ATP levels were not statistically different from each other (p > 0.4).
T10309 21268-21272 JJ denotes Wild
T10310 21273-21277 NN denotes type
T10311 21272-21273 HYPH denotes -
T10312 21321-21327 NNS denotes levels
T10313 21278-21281 CC denotes and
T10314 21282-21294 JJ denotes heterozygous
T10315 21295-21305 NN denotes CbCln3Δex7
T10316 21306-21307 NN denotes 8
T10317 21305-21306 HYPH denotes /
T10318 21308-21316 JJ denotes cellular
T10319 21317-21320 NN denotes ATP
T10320 21328-21332 VBD denotes were
T10321 21333-21336 RB denotes not
T10322 21337-21350 RB denotes statistically
T10323 21351-21360 JJ denotes different
T10324 21361-21365 IN denotes from
T10325 21366-21370 DT denotes each
T10326 21371-21376 JJ denotes other
T10327 21377-21378 -LRB- denotes (
T10328 21382-21385 CD denotes 0.4
T10329 21378-21379 NN denotes p
T10330 21380-21381 SYM denotes >
T10331 21385-21386 -RRB- denotes )
T10332 21386-21387 . denotes .
T10333 21387-21467 sentence denotes A representative of triplicate experiments is shown (n = 6 in each experiment).
T10334 21388-21389 DT denotes A
T10335 21390-21404 NN denotes representative
T10336 21434-21439 VBN denotes shown
T10337 21405-21407 IN denotes of
T10338 21408-21418 JJ denotes triplicate
T10339 21419-21430 NNS denotes experiments
T10340 21431-21433 VBZ denotes is
T10341 21440-21441 -LRB- denotes (
T10342 21445-21446 CD denotes 6
T10343 21441-21442 NN denotes n
T10344 21443-21444 SYM denotes =
T10345 21447-21449 IN denotes in
T10346 21450-21454 DT denotes each
T10347 21455-21465 NN denotes experiment
T10348 21465-21466 -RRB- denotes )
T10349 21466-21467 . denotes .
T10350 21467-21540 sentence denotes c. Cell survival following 24-hour hydrogen peroxide treatment is shown.
T10351 21468-21469 LS denotes c
T10352 21534-21539 VBN denotes shown
T10353 21469-21470 . denotes .
T10354 21471-21475 NN denotes Cell
T10355 21476-21484 NN denotes survival
T10356 21485-21494 VBG denotes following
T10357 21495-21497 CD denotes 24
T10358 21498-21502 NN denotes hour
T10359 21497-21498 HYPH denotes -
T10360 21521-21530 NN denotes treatment
T10361 21503-21511 NN denotes hydrogen
T10362 21512-21520 NN denotes peroxide
T10363 21531-21533 VBZ denotes is
T10364 21539-21540 . denotes .
T10365 21540-21650 sentence denotes Homozygous CbCln3Δex7/8 cells were ~2-fold more sensitive to oxidative stress by hydrogen peroxide treatment.
T10366 21541-21551 JJ denotes Homozygous
T10367 21565-21570 NNS denotes cells
T10368 21552-21562 NN denotes CbCln3Δex7
T10369 21562-21563 HYPH denotes /
T10370 21563-21564 CD denotes 8
T10371 21571-21575 VBD denotes were
T10372 21576-21577 SYM denotes ~
T10373 21577-21583 RB denotes 2-fold
T10374 21584-21588 RBR denotes more
T10375 21589-21598 JJ denotes sensitive
T10376 21599-21601 IN denotes to
T10377 21602-21611 JJ denotes oxidative
T10378 21612-21618 NN denotes stress
T10379 21619-21621 IN denotes by
T10380 21622-21630 NN denotes hydrogen
T10381 21631-21639 NN denotes peroxide
T10382 21640-21649 NN denotes treatment
T10383 21649-21650 . denotes .
T10384 21650-21857 sentence denotes Wild-type (circle) and heterozygous (triangle) CbCln3Δex7/8 cells exhibited ~50% survival rates with 75–100 μM H2O2, whereas homozygous CbCln3Δex7/8 cells (squares) had a ~50% survival rate with 50 μM H2O2.
T10385 21651-21655 JJ denotes Wild
T10386 21656-21660 NN denotes type
T10387 21655-21656 HYPH denotes -
T10388 21711-21716 NNS denotes cells
T10389 21661-21662 -LRB- denotes (
T10390 21662-21668 NN denotes circle
T10391 21668-21669 -RRB- denotes )
T10392 21670-21673 CC denotes and
T10393 21674-21686 JJ denotes heterozygous
T10394 21687-21688 -LRB- denotes (
T10395 21688-21696 NN denotes triangle
T10396 21696-21697 -RRB- denotes )
T10397 21698-21708 NN denotes CbCln3Δex7
T10398 21708-21709 HYPH denotes /
T10399 21709-21710 CD denotes 8
T10400 21717-21726 VBD denotes exhibited
T10401 21727-21728 SYM denotes ~
T10402 21728-21730 CD denotes 50
T10403 21730-21731 NN denotes %
T10404 21741-21746 NNS denotes rates
T10405 21732-21740 NN denotes survival
T10406 21747-21751 IN denotes with
T10407 21752-21754 CD denotes 75
T10408 21755-21758 CD denotes 100
T10409 21754-21755 SYM denotes –
T10410 21759-21761 NN denotes μM
T10411 21762-21766 NN denotes H2O2
T10412 21766-21768 , denotes ,
T10413 21768-21775 IN denotes whereas
T10414 21816-21819 VBD denotes had
T10415 21776-21786 JJ denotes homozygous
T10416 21800-21805 NNS denotes cells
T10417 21787-21797 NN denotes CbCln3Δex7
T10418 21797-21798 HYPH denotes /
T10419 21798-21799 CD denotes 8
T10420 21806-21807 -LRB- denotes (
T10421 21807-21814 NNS denotes squares
T10422 21814-21815 -RRB- denotes )
T10423 21820-21821 DT denotes a
T10424 21836-21840 NN denotes rate
T10425 21822-21823 SYM denotes ~
T10426 21823-21825 CD denotes 50
T10427 21825-21826 NN denotes %
T10428 21827-21835 NN denotes survival
T10429 21841-21845 IN denotes with
T10430 21846-21848 CD denotes 50
T10431 21849-21851 NN denotes μM
T10432 21852-21856 NN denotes H2O2
T10433 21856-21857 . denotes .
T10434 21857-21937 sentence denotes A representative of triplicate experiments is shown (n = 4 in each experiment).
T10435 21858-21859 DT denotes A
T10436 21860-21874 NN denotes representative
T10437 21904-21909 VBN denotes shown
T10438 21875-21877 IN denotes of
T10439 21878-21888 JJ denotes triplicate
T10440 21889-21900 NNS denotes experiments
T10441 21901-21903 VBZ denotes is
T10442 21910-21911 -LRB- denotes (
T10443 21915-21916 CD denotes 4
T10444 21911-21912 NN denotes n
T10445 21913-21914 SYM denotes =
T10446 21917-21919 IN denotes in
T10447 21920-21924 DT denotes each
T10448 21925-21935 NN denotes experiment
T10449 21935-21936 -RRB- denotes )
T10450 21936-21937 . denotes .
T5059 25411-25412 HYPH denotes /
T5047 25325-25328 DT denotes The
T5048 25338-25349 NN denotes trafficking
T5049 25329-25337 NN denotes membrane
T5050 25420-25423 VBP denotes are
T5051 25350-25353 CC denotes and
T5052 25354-25367 JJ denotes mitochondrial
T5053 25368-25376 NNS denotes deficits
T5054 25377-25386 VBN denotes uncovered
T5055 25387-25389 IN denotes in
T5056 25390-25400 JJ denotes homozygous
T5057 25414-25419 NNS denotes cells
T5058 25401-25411 NN denotes CbCln3Δex7
T5060 25412-25413 CD denotes 8
T5061 25424-25430 JJ denotes likely
T5062 25431-25433 TO denotes to
T5063 25447-25453 VB denotes impact
T5064 25434-25446 RB denotes particularly
T5065 25454-25462 JJ denotes neuronal
T5066 25463-25471 NN denotes function
T5067 25471-25472 . denotes .
T5068 25472-25629 sentence denotes Neurotransmission heavily relies on membrane vesicle transport, and a high-energy metabolism may further sensitize neurons to the loss of battenin activity.
T5069 25473-25490 NN denotes Neurotransmission
T5070 25499-25505 VBZ denotes relies
T5071 25491-25498 RB denotes heavily
T5072 25506-25508 IN denotes on
T5073 25509-25517 NN denotes membrane
T5074 25518-25525 NN denotes vesicle
T3675 17896-17898 IN denotes by
T3803 19142-19143 sentence denotes c
T3804 19142-19143 NN denotes c
T3805 19143-19317 sentence denotes hondrial protein and its turnover proceeds through autophagic engulfment of mitochondria [13], we analyzed homozygous CbCln3Δex7/8 cell mitochondrial morphology and function.
T3806 19241-19249 VBD denotes analyzed
T3807 19152-19159 NN denotes protein
T3808 19143-19151 JJ denotes hondrial
T187 137-151 NN denotes lipofuscinosis
T4957 25050-25051 DT denotes a
T4958 25052-25058 NN denotes subset
T4959 25059-25061 IN denotes of
T4960 25062-25071 JJ denotes vesicular
T4961 25072-25084 NNS denotes compartments
T2791 13321-13325 JJ denotes wild
T2792 13326-13330 NN denotes type
T2793 13325-13326 HYPH denotes -
T2794 13331-13339 NNS denotes extracts
T2795 13339-13340 . denotes .
T2796 13340-13459 sentence denotes The cellular growth media did not contain altered levels of cathepsin D, indicating enzyme secretion was not affected.
T2797 13341-13344 DT denotes The
T2798 13361-13366 NNS denotes media
T2799 13345-13353 JJ denotes cellular
T2800 13354-13360 NN denotes growth
T2801 13375-13382 VB denotes contain
T2802 13367-13370 VBD denotes did
T2803 13371-13374 RB denotes not
T2804 13383-13390 VBN denotes altered
T2805 13391-13397 NNS denotes levels
T2806 13398-13400 IN denotes of
T2807 13401-13410 NN denotes cathepsin
T2808 13411-13412 NN denotes D
T2809 13412-13414 , denotes ,
T2810 13414-13424 VBG denotes indicating
T2811 13425-13431 NN denotes enzyme
T2812 13432-13441 NN denotes secretion
T2813 13450-13458 VBN denotes affected
T2814 13442-13445 VBD denotes was
T2815 13446-13449 RB denotes not
T2816 13458-13459 . denotes .
T2817 13459-13614 sentence denotes Heterozygous Cln3Δex7/8 mice and CbCln3Δex7/8 cells were indistinguishable from wild-type, as expected for a recessive disease phenotype (data not shown).
T2818 13460-13472 JJ denotes Heterozygous
T2819 13484-13488 NNS denotes mice
T2820 13473-13481 NN denotes Cln3Δex7
T2821 13481-13482 HYPH denotes /
T2822 13482-13483 CD denotes 8
T2823 13512-13516 VBD denotes were
T2824 13489-13492 CC denotes and
T2825 13493-13503 NN denotes CbCln3Δex7
T2826 13506-13511 NNS denotes cells
T2827 13503-13504 HYPH denotes /
T2828 13504-13505 CD denotes 8
T2829 13517-13534 JJ denotes indistinguishable
T2830 13535-13539 IN denotes from
T2831 13540-13544 JJ denotes wild
T2832 13545-13549 NN denotes type
T2833 13544-13545 HYPH denotes -
T2834 13549-13551 , denotes ,
T2835 13551-13553 IN denotes as
T2836 13554-13562 VBN denotes expected
T2837 13563-13566 IN denotes for
T2849 14749-14768 sentence denotes The impact of the a
T2850 14749-14752 DT denotes The
T2851 14753-14754 NN denotes i
T2852 14754-14755 JJ denotes m
T2853 14755-14756 NN denotes p
T2854 14756-14757 CC denotes a
T2855 14757-14758 JJ denotes c
T2856 14758-14759 NNS denotes t
T2857 14759-14760 , denotes
T2858 14760-14761 JJR denotes o
T2859 14761-14762 IN denotes f
T2860 14763-14764 JJ denotes t
T2861 14764-14765 NN denotes h
T2862 14765-14766 JJ denotes e
T2863 14767-14768 NN denotes a
T2864 14768-14780 sentence denotes ltered cathe
T2865 14768-14769 RB denotes l
T2866 14772-14774 VBD denotes ed
T2867 14770-14772 NNS denotes er
T2868 14769-14770 NN denotes t
T2869 14775-14776 VBN denotes c
T2870 14776-14777 CC denotes a
T2871 14777-14780 DT denotes the
T2872 14780-14923 sentence denotes psin D processing on enzymatic activity was next tested to determine if altered enzymatic activity accounts for inefficient subunit c turnover.
T2873 14780-14781 NN denotes p
T2874 14829-14835 VBN denotes tested
T2875 14787-14797 NN denotes processing
T2876 14781-14784 NN denotes sin
T2877 14785-14786 NN denotes D
T2878 14798-14800 IN denotes on
T2879 14801-14810 JJ denotes enzymatic
T2880 14811-14819 NN denotes activity
T2881 14820-14823 VBD denotes was
T2882 14824-14828 RB denotes next
T2883 14836-14838 TO denotes to
T2884 14839-14848 VB denotes determine
T2885 14849-14851 IN denotes if
T2886 14879-14887 VBZ denotes accounts
T2887 14852-14859 VBN denotes altered
T2888 14870-14878 NN denotes activity
T4962 25085-25092 VBG denotes linking
T4963 25093-25101 JJ denotes multiple
T2070 7687-7688 HYPH denotes -
T2071 7700-7703 CC denotes and
T2072 7704-7707 DT denotes the
T2073 7715-7721 NN denotes allele
T2074 7708-7714 NN denotes mutant
T2075 7721-7723 , denotes ,
T2076 7723-7726 CC denotes and
T2077 7727-7732 NN denotes batp1
T2078 7733-7739 NN denotes signal
T2079 7740-7743 VBD denotes was
T2080 7744-7751 JJ denotes similar
T2081 7752-7754 IN denotes to
T2082 7755-7759 DT denotes that
T2083 7760-7764 VBN denotes seen
T2084 7765-7767 IN denotes in
T2085 7768-7772 JJ denotes wild
T2086 7773-7777 NN denotes type
T2087 7772-7773 HYPH denotes -
T2088 7778-7783 NNS denotes cells
T2089 7784-7785 -LRB- denotes (
T2090 7794-7799 VBN denotes shown
T2091 7785-7789 NNS denotes data
T2092 7790-7793 RB denotes not
T2093 7799-7800 -RRB- denotes )
T2094 7800-7801 . denotes .
T4964 25123-25131 NNS denotes pathways
T4965 25102-25110 NN denotes membrane
T2095 7801-9594 sentence denotes Figure 2 RT-PCR of Cln3 mRNA in wild-type and homozygous CbCln3Δex7/8 cells Cln3 Exon1-forward, Exon 15-reverse RT-PCR products are shown, from total wild-type (+/+) or homozygous mutant (Δex7/8/Δex7/8) brain and cell line RNA. Brain and cell line RT-PCR reaction products had identical band patterns on ethidium-bromide stained agarose gels. Wild-type RT-PCR product was a single ~1.6 kb band and mutant products were ~1.6, ~1.5, ~1.4, ~1.35, and ~1.3 kb, representing multiple mutant splice variants. Figure 3 Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. During sub-confluent growth conditions, neither wild-type nor homozygous CbCln3Δex7/8 cells displayed autofluorescence or subunit c inclusion formation (data not shown).
T2096 9425-9431 IN denotes During
T2097 9517-9526 VBD denotes displayed
T2098 9432-9445 JJ denotes sub-confluent
T2099 9453-9463 NNS denotes conditions
T2100 9446-9452 NN denotes growth
T2101 9463-9465 , denotes ,
T2102 9465-9472 CC denotes neither
T2103 9478-9482 NN denotes type
T2104 9473-9477 JJ denotes wild
T2105 9477-9478 HYPH denotes -
T2106 9511-9516 NNS denotes cells
T2107 9483-9486 CC denotes nor
T2108 9487-9497 JJ denotes homozygous
T2109 9498-9508 NN denotes CbCln3Δex7
T2110 9508-9509 HYPH denotes /
T2111 9509-9510 CD denotes 8
T2112 9527-9543 NN denotes autofluorescence
T2113 9567-9576 NN denotes formation
T2114 9544-9546 CC denotes or
T2115 9547-9554 NN denotes subunit
T2116 9555-9556 NN denotes c
T2117 9557-9566 NN denotes inclusion
T2118 9577-9578 -LRB- denotes (
T2119 9587-9592 VBN denotes shown
T2120 9578-9582 NNS denotes data
T2121 9583-9586 RB denotes not
T2122 9592-9593 -RRB- denotes )
T2123 9593-9594 . denotes .
T2124 9594-9823 sentence denotes However, when cells were aged at confluency (3+ days post-confluency), homozygous CbCln3Δex7/8 cellular subunit c levels were elevated beyond normal wild-type levels by immunostaining (Fig. 4a) and immunoblot analysis (Fig. 4b).
T2125 9595-9602 RB denotes However
T2126 9721-9729 VBN denotes elevated
T2127 9602-9604 , denotes ,
T4966 25111-25122 NN denotes trafficking
T4967 25131-25133 , denotes ,
T4968 25133-25140 RB denotes perhaps
T3529 16403-16413 RB denotes previously
T3530 16420-16421 -LRB- denotes (
T3531 16421-16425 NN denotes Fig.
T3532 16426-16427 CD denotes 3
T3630 17834-17835 VBN denotes w
T3631 17824-17825 NNS denotes o
T3632 17823-17824 NN denotes C
T3633 17825-17826 NN denotes n
T3634 17826-17827 JJ denotes s
T3635 17827-17828 IN denotes i
T3636 17828-17831 NN denotes ste
T3637 17832-17833 NN denotes t
T3638 17831-17832 CC denotes n
T3639 17835-17840 sentence denotes ith t
T3640 17835-17836 JJ denotes i
T3641 17836-17837 VBN denotes t
T3642 17837-17838 DT denotes h
T3643 17839-17840 NN denotes t
T3644 17840-17849 sentence denotes he altere
T3645 17840-17841 JJ denotes h
T3646 17841-17842 NNS denotes e
T3647 17843-17845 JJR denotes al
T3648 17845-17846 JJ denotes t
T3649 17846-17849 JJ denotes ere
T3650 17849-17861 sentence denotes d early endo
T3651 17849-17850 VBD denotes d
T3652 17851-17852 VBN denotes e
T3653 17852-17853 NN denotes a
T3654 17853-17855 JJ denotes rl
T3655 17855-17856 JJ denotes y
T3656 17857-17858 NN denotes e
T3657 17858-17859 IN denotes n
T3658 17859-17860 VBN denotes d
T3659 17860-17861 NN denotes o
T3660 17861-17866 sentence denotes some
T3661 17863-17865 NNS denotes me
T3662 17861-17862 JJ denotes s
T3663 17862-17863 JJ denotes o
T3664 17866-17868 sentence denotes ma
T3665 17866-17868 NN denotes ma
T3666 17868-18043 sentence denotes rker (EEA1) signal observed by immunostaining (Fig. 3), fluid-phase endocytosis was also altered in homozygous CbCln3Δex7/8 cells, as measured by dextran-FITC uptake (Fig. 7).
T3667 17957-17964 VBN denotes altered
T3668 17880-17886 NN denotes signal
T3669 17868-17869 JJ denotes r
T3670 17869-17872 NN denotes ker
T3671 17873-17874 -LRB- denotes (
T3672 17874-17878 NN denotes EEA1
T3673 17878-17879 -RRB- denotes )
T3674 17887-17895 VBN denotes observed
T3676 17899-17913 NN denotes immunostaining
T3677 17914-17915 -LRB- denotes (
T3678 17915-17919 NN denotes Fig.
T3679 17920-17921 CD denotes 3
T3680 17921-17922 -RRB- denotes )
T3681 17922-17924 , denotes ,
T3682 17924-17929 NN denotes fluid
T3683 17930-17935 NN denotes phase
T3684 17929-17930 HYPH denotes -
T3685 17936-17947 NN denotes endocytosis
T3686 17948-17951 VBD denotes was
T3687 17952-17956 RB denotes also
T3688 17965-17967 IN denotes in
T3689 17968-17978 JJ denotes homozygous
T3690 17992-17997 NNS denotes cells
T3691 17979-17989 NN denotes CbCln3Δex7
T3692 17989-17990 HYPH denotes /
T3693 17990-17991 CD denotes 8
T3694 17997-17999 , denotes ,
T3714 18058-18059 HYPH denotes -
T3715 18077-18079 IN denotes in
T3716 18080-18085 NNS denotes media
T3717 18086-18096 VBG denotes containing
T3718 18097-18104 NN denotes dextran
T3719 18105-18109 NN denotes FITC
T3720 18104-18105 HYPH denotes -
T3721 18109-18111 , denotes ,
T3722 18111-18115 JJ denotes wild
T3723 18116-18120 NN denotes type
T3724 18115-18116 HYPH denotes -
T3725 18138-18143 NNS denotes cells
T3726 18121-18124 CC denotes and
T3727 18125-18137 NN denotes heterozygote
T3728 18154-18162 RB denotes brightly
T3729 18163-18170 VBN denotes stained
T3730 18188-18196 NNS denotes vesicles
T3731 18170-18172 , denotes ,
T3732 18172-18177 JJ denotes large
T3733 18178-18187 JJ denotes endocytic
T3734 18197-18201 WDT denotes that
T3735 18207-18216 VBN denotes clustered
T3736 18202-18206 VBD denotes were
T3737 18217-18219 IN denotes in
T3738 18220-18223 DT denotes the
T3739 18236-18242 NN denotes region
T3740 18224-18235 JJ denotes perinuclear
T3741 18242-18243 . denotes .
T3742 18243-18418 sentence denotes However, homozygous CbCln3Δex7/8 cells were less brightly stained with most dextran-FITC signal localizing to smaller vesicles scattered throughout the cytoplasm of the cell.
T3743 18244-18251 RB denotes However
T3744 18302-18309 VBN denotes stained
T3745 18251-18253 , denotes ,
T3746 18253-18263 JJ denotes homozygous
T3747 18277-18282 NNS denotes cells
T3748 18264-18274 NN denotes CbCln3Δex7
T3785 19118-19119 NN denotes a
T3786 19119-19121 JJ denotes us
T3787 19121-19122 NN denotes e
T3788 19122-19123 , denotes
T3789 19124-19125 JJ denotes u
T3790 19125-19126 VBN denotes b
T3791 19126-19138 sentence denotes unit c is a
T3792 19126-19127 JJ denotes u
T3793 19127-19128 NN denotes n
T3794 19131-19132 NNS denotes c
T3795 19128-19130 JJ denotes it
T3796 19133-19134 VBD denotes i
T3797 19134-19135 JJR denotes s
T3798 19136-19137 VBN denotes a
T3799 19138-19142 sentence denotes mito
T3800 19138-19139 NNS denotes m
T3801 19139-19141 IN denotes it
T3802 19141-19142 NN denotes o
T4969 25141-25152 VBG denotes functioning
T4945 24985-24987 IN denotes in
T4946 24988-24995 NNS denotes neurons
T4947 24995-24996 . denotes .
T4948 24996-25190 sentence denotes These data jointly indicate that battenin resides in a subset of vesicular compartments linking multiple membrane trafficking pathways, perhaps functioning in vesicular transport and/or fusion.
T4949 24997-25002 DT denotes These
T4950 25003-25007 NNS denotes data
T4951 25016-25024 VBP denotes indicate
T4952 25008-25015 RB denotes jointly
T4953 25025-25029 IN denotes that
T4954 25039-25046 VBZ denotes resides
T4955 25030-25038 NN denotes battenin
T4956 25047-25049 IN denotes in
T4970 25153-25155 IN denotes in
R4100 T6713 T6712 nummod 7.4,pH
R4101 T6714 T6706 punct ", ",formaldehyde
R4102 T6715 T6706 prep for,formaldehyde
R4103 T6716 T6717 nummod 20,minutes
R4104 T6717 T6715 pobj minutes,for
R4105 T6718 T6702 punct ", ",with
R4106 T6719 T6702 cc or,with
R4108 T6721 T6722 npadvmod ice,cold
R4109 T6722 T6724 amod cold,acetone
R4110 T6723 T6722 punct -,cold
R4111 T6724 T6720 pobj acetone,with
R4112 T6725 T6724 compound methanol,acetone
R4113 T6726 T6724 punct /,acetone
R4114 T6727 T6728 punct (,1
R4115 T6728 T6724 parataxis 1,acetone
R4116 T6729 T6730 punct :,1
R4117 T6730 T6728 prep 1,1
R4252 T6871 T6870 compound mounting,medium
R4253 T6872 T6873 punct (,Laboratories
R4254 T6873 T6870 parataxis Laboratories,medium
R4255 T6874 T6873 compound Vector,Laboratories
R4256 T6875 T6873 punct ),Laboratories
R4257 T6876 T6862 cc and,coverslipped
R4258 T6877 T6862 conj analyzed,coverslipped
R4259 T6878 T6877 prep on,analyzed
R4260 T6879 T6880 det a,microscope
R4261 T6880 T6878 pobj microscope,on
R4262 T6881 T6882 nmod BioRad,Radiance
R4263 T6882 T6880 nmod Radiance,microscope
R4264 T6883 T6882 nummod 2100,Radiance
R5964 T9402 T9395 punct ", ",band
R5965 T9403 T9395 acl representing,band
R5966 T9404 T9403 dobj precursor,representing
R5967 T9405 T9401 punct ", ",was
R5968 T9406 T9407 det the,band
R5969 T9407 T9401 attr band,was
R5970 T9408 T9407 amod predominant,band
R5971 T9409 T9407 prep in,band
R6097 T9631 T9630 punct -,clustered
R6098 T9632 T9627 cc and,lysosomes
R6099 T9633 T9634 amod scattered,lysosomes
R6100 T9634 T9627 conj lysosomes,lysosomes
R6101 T9635 T9621 prep in,labeled
R6156 T9694 T9695 advmod less,clustering
R6157 T9695 T9693 pobj clustering,with
R6158 T9696 T9695 amod perinuclear,clustering
R6202 T9820 T9822 nmod type,cells
R6408 T10188 T10186 punct x,2.75
R6409 T10189 T10183 punct ),insets
R6410 T10190 T10141 punct .,highlighted
R6411 T10192 T10193 amod Mitochondrial,distribution
R6521 T10312 T10320 nsubj levels,were
R6522 T10313 T10310 cc and,type
R6523 T10314 T10310 conj heterozygous,type
R6524 T10315 T10316 nmod CbCln3Δex7,8
R6525 T10316 T10312 nmod 8,levels
R6526 T10317 T10316 punct /,8
R6527 T10318 T10319 amod cellular,ATP
R6528 T10319 T10312 compound ATP,levels
R6529 T10321 T10320 neg not,were
R6530 T10322 T10323 advmod statistically,different
R6531 T10323 T10320 acomp different,were
R6549 T10343 T10342 nsubj n,6
R6550 T10344 T10342 punct =,6
R6551 T10345 T10342 prep in,6
R6552 T10346 T10347 det each,experiment
R6553 T10347 T10345 pobj experiment,in
R6554 T10348 T10342 punct ),6
R6555 T10349 T10336 punct .,shown
R6556 T10351 T10352 meta c,shown
R6557 T10353 T10351 punct .,c
R6558 T10354 T10355 compound Cell,survival
R6559 T10355 T10352 nsubjpass survival,shown
R6560 T10356 T10355 prep following,survival
R6561 T10357 T10358 nummod 24,hour
R6562 T10358 T10360 compound hour,treatment
R6563 T10359 T10358 punct -,hour
R6564 T10360 T10356 pobj treatment,following
R6565 T10361 T10362 compound hydrogen,peroxide
R6566 T10362 T10360 compound peroxide,treatment
R6567 T10363 T10352 auxpass is,shown
R6568 T10364 T10352 punct .,shown
R6569 T10366 T10367 amod Homozygous,cells
R6570 T10367 T10371 nsubj cells,were
R6571 T10368 T10367 nmod CbCln3Δex7,cells
R6572 T10369 T10368 punct /,CbCln3Δex7
R6573 T10370 T10368 nummod 8,CbCln3Δex7
R6574 T10372 T10373 punct ~,2-fold
R6575 T10373 T10374 advmod 2-fold,more
R6576 T10374 T10375 advmod more,sensitive
R6577 T10375 T10371 acomp sensitive,were
R6578 T10376 T10375 prep to,sensitive
R6579 T10377 T10378 amod oxidative,stress
R6580 T10378 T10376 pobj stress,to
R6581 T10379 T10378 prep by,stress
R6582 T10380 T10381 compound hydrogen,peroxide
R6604 T10404 T10400 dobj rates,exhibited
R6605 T10405 T10404 compound survival,rates
R6606 T10406 T10404 prep with,rates
R6607 T10407 T10408 quantmod 75,100
R6608 T10408 T10410 nummod 100,μM
R6609 T10409 T10408 punct –,100
R6610 T10410 T10411 compound μM,H2O2
R6611 T10411 T10406 pobj H2O2,with
R6612 T10412 T10400 punct ", ",exhibited
R6613 T10413 T10414 mark whereas,had
R6614 T10414 T10400 advcl had,exhibited
R6615 T10415 T10416 amod homozygous,cells
R6616 T10416 T10414 nsubj cells,had
R6623 T10423 T10424 det a,rate
R6624 T10424 T10414 dobj rate,had
R6625 T10425 T10426 punct ~,50
R6626 T10426 T10427 nummod 50,%
R6627 T10427 T10424 compound %,rate
R6628 T10428 T10424 compound survival,rate
R6629 T10429 T10424 prep with,rate
R6630 T10430 T10431 nummod 50,μM
R6631 T10431 T10432 compound μM,H2O2
R6632 T10432 T10429 pobj H2O2,with
R85 T264 T261 cc and,express
R86 T265 T261 conj display,express
R3966 T6475 T6448 conj processed,fixed
R3967 T6476 T6475 prep for,processed
R3968 T6477 T6476 pobj autofluorescence,for
R3969 T6478 T6477 punct /,autofluorescence
R3970 T6479 T6480 compound subunit,c
R3971 T6480 T6481 compound c,immunostaining
R3972 T6481 T6477 appos immunostaining,autofluorescence
R3973 T6482 T6448 punct ", ",fixed
R3974 T6483 T6448 cc or,fixed
R3975 T6484 T6485 compound cell,pellets
R3976 T6485 T6486 nsubjpass pellets,collected
R3977 T6486 T6448 conj collected,fixed
R3978 T6487 T6486 auxpass were,collected
R3979 T6488 T6486 prep for,collected
R3980 T6489 T6490 amod total,extraction
R3981 T6490 T6488 pobj extraction,for
R3982 T6491 T6490 compound protein,extraction
R3983 T6492 T6486 punct .,collected
R3984 T6494 T6495 advmod Alternatively,fixed
R3985 T6496 T6495 punct ", ",fixed
R3986 T6497 T6495 nsubjpass cells,fixed
R3987 T6498 T6495 auxpass were,fixed
R3988 T6499 T6495 prep with,fixed
R3989 T6500 T6501 nummod 2.5,%
R3990 T6501 T6502 compound %,glutaraldehyde
R3991 T6502 T6499 pobj glutaraldehyde,with
R3992 T6503 T6502 punct /,glutaraldehyde
R3993 T6504 T6505 nummod 2,%
R3994 T6505 T6506 compound %,paraformaldehyde
R3995 T6506 T6502 appos paraformaldehyde,glutaraldehyde
R3996 T6507 T6495 prep in,fixed
R3997 T6508 T6509 nummod 0.1,M
R3998 T6509 T6510 compound M,buffer
R3999 T6510 T6507 pobj buffer,in
R4000 T6511 T6510 compound cacodylate,buffer
R4001 T6512 T6510 punct ", ",buffer
R4050 T6658 T6659 nmod Immunostaining,analysis
R4051 T6660 T6658 cc and,Immunostaining
R4052 T6661 T6658 conj Immunoblot,Immunostaining
R4053 T6663 T6664 prep For,seeded
R4054 T6665 T6663 pobj immunostaining,For
R4055 T6666 T6664 punct ", ",seeded
R4056 T6667 T6664 nsubj cells,seeded
R4057 T6668 T6664 aux were,seeded
R4058 T6669 T6664 prep at,seeded
R4059 T6670 T6671 det a,density
R4060 T6671 T6669 pobj density,at
R4061 T6672 T6671 prep of,density
R4062 T6673 T6674 quantmod 3,5
R4063 T6674 T6676 quantmod 5,104
R4064 T6675 T6674 punct –,5
R4065 T6676 T6678 nummod 104,cells
R4066 T6677 T6676 punct ×,104
R4067 T6678 T6672 pobj cells,of
R4068 T6679 T6678 prep per,cells
R4069 T6680 T6679 pobj well,per
R4070 T6681 T6664 prep in,seeded
R4071 T6682 T6683 nummod 4,well
R4072 T6683 T6685 compound well,slides
R4073 T6684 T6683 punct -,well
R4074 T6685 T6681 pobj slides,in
R4075 T6686 T6685 compound chamber,slides
R4076 T6687 T6685 punct -,slides
R4077 T6688 T6664 cc and,seeded
R4078 T6689 T6664 conj grown,seeded
R4079 T6690 T6689 advmod overnight,grown
R4080 T6691 T6689 prep at,grown
R4081 T6692 T6693 nummod 33,°C
R4082 T6693 T6691 pobj °C,at
R4083 T6694 T6664 punct ", ",seeded
R4084 T6695 T6696 mark unless,indicated
R4085 T6696 T6664 advcl indicated,seeded
R4086 T6697 T6696 advmod otherwise,indicated
R4087 T6698 T6664 punct .,seeded
R4088 T6700 T6701 nsubj Fixation,was
R4089 T6702 T6701 prep with,was
R4090 T6703 T6704 npadvmod ice,cold
R4091 T6704 T6706 amod cold,formaldehyde
R4092 T6705 T6704 punct -,cold
R4093 T6706 T6702 pobj formaldehyde,with
R4094 T6707 T6708 nummod 4,%
R4095 T6708 T6706 compound %,formaldehyde
R4096 T6709 T6706 prep in,formaldehyde
R4097 T6710 T6709 pobj PBS,in
R4098 T6711 T6710 punct ", ",PBS
R4099 T6712 T6710 npadvmod pH,PBS
R4107 T6720 T6702 conj with,with
R4118 T6731 T6728 punct ),1
R4119 T6732 T6724 prep for,acetone
R4120 T6733 T6734 nummod 10,minutes
R4121 T6734 T6732 pobj minutes,for
R4122 T6735 T6724 prep at,acetone
R4123 T6736 T6737 punct -,20
R4124 T6737 T6738 nummod 20,°C
R4125 T6738 T6735 pobj °C,at
R4126 T6739 T6724 acl followed,acetone
R4127 T6740 T6739 agent by,followed
R4128 T6741 T6742 compound air,drying
R4129 T6742 T6740 pobj drying,by
R4130 T6743 T6742 punct -,drying
R4131 T6744 T6745 punct (,dependent
R4132 T6745 T6739 parataxis dependent,followed
R4133 T6746 T6745 npadvmod antibody,dependent
R4134 T6747 T6745 punct -,dependent
R4135 T6748 T6745 punct ),dependent
R4136 T6749 T6701 punct .,was
R4137 T6751 T6752 nsubjpass Cells,washed
R4138 T6753 T6752 auxpass were,washed
R4139 T6754 T6752 prep with,washed
R4140 T6755 T6754 pobj PBS,with
R4141 T6756 T6757 advmod at,2
R4142 T6757 T6752 npadvmod 2,washed
R4143 T6758 T6757 advmod least,2
R4144 T6759 T6757 quantmod times,2
R4145 T6760 T6752 punct ", ",washed
R4146 T6761 T6762 nummod 5,minutes
R4147 T6762 T6752 npadvmod minutes,washed
R4148 T6763 T6762 prep per,minutes
R4149 T6764 T6763 pobj wash,per
R4150 T6765 T6752 punct ", ",washed
R4151 T6766 T6752 prep between,washed
R4152 T6767 T6766 pobj each,between
R4153 T6768 T6767 prep of,each
R4154 T6769 T6770 det the,steps
R4155 T6770 T6768 pobj steps,of
R4156 T6771 T6770 amod following,steps
R4157 T6772 T6770 prep of,steps
R4158 T6773 T6774 det the,procedure
R4159 T6774 T6772 pobj procedure,of
R4160 T6775 T6774 compound staining,procedure
R4161 T6776 T6770 punct : ,steps
R4162 T6777 T6778 nummod 0.1,M
R4163 T6778 T6779 compound M,glycine
R4164 T6779 T6770 appos glycine,steps
R4165 T6780 T6779 prep in,glycine
R4166 T6781 T6780 pobj PBS,in
R4167 T6782 T6779 prep for,glycine
R4168 T6783 T6784 nummod 5,minutes
R4169 T6784 T6782 pobj minutes,for
R4170 T6785 T6779 punct ", ",glycine
R4171 T6786 T6787 nummod 0.05,%
R4172 T6787 T6788 nmod %,X
R4173 T6788 T6779 appos X,glycine
R4174 T6789 T6787 cc or,%
R4175 T6790 T6791 nummod 0.1,%
R4176 T6791 T6787 conj %,%
R4177 T6792 T6788 punct (,X
R4178 T6793 T6794 npadvmod antibody,dependent
R4179 T6794 T6788 amod dependent,X
R4180 T6795 T6794 punct -,dependent
R4181 T6796 T6788 punct ),X
R4182 T6797 T6788 compound Triton,X
R4183 T6798 T6788 punct -,X
R4184 T6799 T6788 nummod 100,X
R4185 T6800 T6801 punct (,Scientific
R4186 T6801 T6788 parataxis Scientific,X
R4187 T6802 T6801 compound Fisher,Scientific
R4188 T6803 T6801 punct ),Scientific
R4189 T6804 T6788 prep in,X
R4190 T6805 T6804 pobj PBS,in
R4191 T6806 T6788 prep for,X
R4192 T6807 T6808 nummod 5,minutes
R4193 T6808 T6806 pobj minutes,for
R4194 T6809 T6779 punct ", ",glycine
R4195 T6810 T6811 nummod 2,%
R4196 T6811 T6779 appos %,glycine
R4197 T6812 T6813 amod bovine,albumin
R4198 T6813 T6811 appos albumin,%
R4199 T6814 T6813 compound serum,albumin
R4200 T6815 T6813 punct (,albumin
R4201 T6816 T6813 appos BSA,albumin
R4202 T6817 T6811 punct ),%
R4203 T6818 T6811 prep in,%
R4204 T6819 T6818 pobj PBS,in
R4205 T6820 T6811 prep for,%
R4206 T6821 T6822 nummod 30,minutes
R4207 T6822 T6820 pobj minutes,for
R4208 T6823 T6779 punct ", ",glycine
R4209 T6824 T6825 amod primary,antibody
R4210 T6825 T6779 appos antibody,glycine
R4211 T6826 T6825 acl diluted,antibody
R4212 T6827 T6826 prep in,diluted
R4213 T6828 T6829 nummod 2,%
R4214 T6829 T6830 compound %,PBS
R4215 T6830 T6827 pobj PBS,in
R4216 T6831 T6830 compound BSA,PBS
R4217 T6832 T6830 punct /,PBS
R4218 T6833 T6826 prep for,diluted
R4219 T6834 T6835 nummod 90,minutes
R4220 T6835 T6833 pobj minutes,for
R4221 T6836 T6779 punct ", ",glycine
R4222 T6837 T6838 amod secondary,antibody
R4223 T6838 T6779 appos antibody,glycine
R4224 T6839 T6838 acl diluted,antibody
R4225 T6840 T6839 prep in,diluted
R4226 T6841 T6842 nummod 2,%
R4227 T6842 T6843 compound %,PBS
R4228 T6843 T6840 pobj PBS,in
R4229 T6844 T6843 compound BSA,PBS
R4230 T6845 T6843 punct /,PBS
R4231 T6846 T6839 prep for,diluted
R4232 T6847 T6848 nummod 60,minutes
R4233 T6848 T6846 pobj minutes,for
R4234 T6849 T6752 punct .,washed
R4235 T6851 T6852 det All,incubations
R4236 T6852 T6853 nsubjpass incubations,carried
R4237 T6854 T6853 auxpass were,carried
R4238 T6855 T6853 prt out,carried
R4239 T6856 T6853 prep at,carried
R4240 T6857 T6858 compound room,temperature
R4241 T6858 T6856 pobj temperature,at
R4242 T6859 T6853 punct .,carried
R4243 T6861 T6862 prep Following,coverslipped
R4244 T6863 T6864 compound staining,procedures
R4245 T6864 T6861 pobj procedures,Following
R4246 T6865 T6862 punct ", ",coverslipped
R4247 T6866 T6862 nsubjpass slides,coverslipped
R4248 T6867 T6862 auxpass were,coverslipped
R4249 T6868 T6862 prep with,coverslipped
R4250 T6869 T6870 compound Vectashield,medium
R4251 T6870 T6868 pobj medium,with
R4265 T6884 T6880 amod confocal,microscope
R4266 T6885 T6886 punct (,Biorad
R4267 T6886 T6880 parataxis Biorad,microscope
R4268 T6887 T6886 punct ),Biorad
R4269 T6888 T6877 punct ", ",analyzed
R4270 T6889 T6877 prep with,analyzed
R4271 T6890 T6891 amod identical,settings
R4272 T6891 T6889 pobj settings,with
R4273 T6892 T6891 compound exposure,settings
R4274 T6893 T6891 prep for,settings
R4275 T6894 T6895 amod wild,type
R4276 T6895 T6897 nmod type,images
R4277 T6896 T6895 punct -,type
R4278 T6897 T6893 pobj images,for
R4279 T6898 T6895 cc and,type
R4280 T6899 T6895 conj mutant,type
R4281 T6900 T6897 amod like,images
R4282 T6901 T6862 punct .,coverslipped
R4283 T6903 T6904 det All,comparisons
R4284 T6904 T6905 nsubjpass comparisons,performed
R4285 T6906 T6904 prep of,comparisons
R4286 T6907 T6908 amod wild,type
R4287 T6908 T6910 nmod type,staining
R4288 T6909 T6908 punct -,type
R4289 T6910 T6906 pobj staining,of
R4290 T6911 T6908 cc and,type
R4291 T6912 T6908 conj mutant,type
R4292 T6913 T6905 auxpass were,performed
R4293 T6914 T6905 prep in,performed
R4294 T6915 T6916 compound Adobe,Photoshop
R4295 T6916 T6914 pobj Photoshop,in
R4296 T6917 T6905 prep with,performed
R4297 T6918 T6919 amod identical,adjustments
R4298 T6919 T6917 pobj adjustments,with
R4299 T6920 T6919 nmod brightness,adjustments
R4300 T6921 T6920 cc and,brightness
R4301 T6922 T6920 conj contrast,brightness
R4302 T6923 T6905 punct .,performed
R4303 T6925 T6926 amod Total,proteins
R4304 T6926 T6927 nsubjpass proteins,isolated
R4305 T6928 T6927 auxpass were,isolated
R4306 T6929 T6927 prep from,isolated
R4307 T6930 T6931 compound cell,pellets
R4308 T6931 T6929 pobj pellets,from
R4309 T6932 T6927 prep by,isolated
R4310 T6933 T6932 pobj extraction,by
R4311 T6934 T6933 prep with,extraction
R4312 T6935 T6936 nmod ice,Tris
R4313 T6936 T6934 pobj Tris,with
R4314 T6937 T6935 punct -,ice
R4315 T6938 T6935 amod cold,ice
R4316 T6939 T6940 nummod 20,mM
R4317 T6940 T6936 compound mM,Tris
R4318 T6941 T6936 punct ", ",Tris
R4319 T6942 T6936 npadvmod pH,Tris
R4320 T6943 T6942 nummod 7.4,pH
R4321 T6944 T6936 punct ", ",Tris
R4322 T6945 T6946 nummod 1,%
R4323 T6946 T6947 compound %,X
R4324 T6947 T6936 conj X,Tris
R4325 T6948 T6947 compound Triton,X
R4326 T6949 T6947 punct -,X
R4327 T6950 T6947 nummod 100,X
R4328 T6951 T6952 punct (,Roche
R4329 T6952 T6947 parataxis Roche,X
R4330 T6953 T6954 compound membrane,research
R4331 T6954 T6956 compound research,grade
R4332 T6955 T6954 punct -,research
R4333 T6956 T6952 dep grade,Roche
R4334 T6957 T6952 punct ", ",Roche
R4335 T6958 T6952 punct ),Roche
R4336 T6959 T6947 punct ", ",X
R4337 T6960 T6947 cc plus,X
R4338 T6961 T6962 compound protease,inhibitors
R4339 T6962 T6947 conj inhibitors,X
R4340 T6963 T6962 punct (,inhibitors
R4341 T6964 T6965 amod Complete,tablet
R4342 T6965 T6962 appos tablet,inhibitors
R4343 T6966 T6965 compound mini,tablet
R4344 T6967 T6965 punct ", ",tablet
R4345 T6968 T6969 nummod 0.7,μg
R4346 T6969 T6970 nmod μg,A
R4347 T6970 T6965 appos A,tablet
R4348 T6971 T6972 punct /,ml
R4349 T6972 T6969 prep ml,μg
R4350 T6973 T6970 compound pepstatin,A
R4351 T6974 T6965 punct ", ",tablet
R4352 T6975 T6976 nummod 2,μg
R4353 T6976 T6977 nmod μg,aprotinin
R4354 T6977 T6965 appos aprotinin,tablet
R4355 T6978 T6979 punct /,ml
R4356 T6979 T6976 prep ml,μg
R4357 T6980 T6965 punct ", ",tablet
R4358 T6981 T6982 nummod 5,μg
R4359 T6982 T6983 nmod μg,leupeptin
R4360 T6983 T6965 appos leupeptin,tablet
R4361 T6984 T6985 punct /,ml
R4362 T6985 T6982 prep ml,μg
R4363 T6986 T6987 punct [,Roche
R4364 T6987 T6983 parataxis Roche,leupeptin
R4365 T6988 T6987 punct ],Roche
R4366 T6989 T6927 punct ),isolated
R4367 T6990 T6927 punct .,isolated
R4368 T6992 T6993 prep Following,centrifuged
R4369 T6994 T6992 pobj homogenization,Following
R4370 T6995 T6994 prep through,homogenization
R4371 T6996 T6997 det a,needle
R4372 T6997 T6995 pobj needle,through
R4373 T6998 T6999 nummod 25,gauge
R4374 T6999 T6997 compound gauge,needle
R4375 T7000 T6999 punct -,gauge
R4376 T7001 T7002 punct (,passes
R4377 T7002 T6994 parataxis passes,homogenization
R4378 T7003 T7004 punct ~,10
R4379 T7004 T7002 nummod 10,passes
R4380 T7005 T7002 punct ),passes
R4381 T7006 T6993 punct ", ",centrifuged
R4382 T7007 T6993 nsubjpass extracts,centrifuged
R4383 T7008 T6993 auxpass were,centrifuged
R4384 T7009 T6993 prep at,centrifuged
R4385 T7010 T7011 nummod 1000,g
R4386 T7011 T7009 pobj g,at
R4387 T7012 T7011 punct ×,g
R4388 T7013 T6993 prep for,centrifuged
R4389 T7014 T7015 nummod 10,minutes
R4390 T7015 T7013 pobj minutes,for
R4391 T7016 T6993 punct ", ",centrifuged
R4392 T7017 T6993 prep at,centrifuged
R4393 T7018 T7019 nummod 4,°C
R4394 T7019 T7017 pobj °C,at
R4395 T7020 T6993 punct ", ",centrifuged
R4396 T7021 T7022 aux to,remove
R4397 T7022 T6993 advcl remove,centrifuged
R4398 T7023 T7022 dobj debris,remove
R4399 T7024 T6993 punct .,centrifuged
R4400 T7026 T7027 advmod Typically,separated
R4401 T7028 T7027 punct ", ",separated
R4402 T7029 T7030 quantmod 20,40
R4403 T7030 T7032 nummod 40,μg
R4404 T7031 T7030 punct –,40
R4405 T7032 T7027 nsubjpass μg,separated
R4406 T7033 T7032 prep of,μg
R4407 T7034 T7033 pobj protein,of
R4408 T7035 T7034 punct (,protein
R4409 T7036 T7034 acl determined,protein
R4410 T7037 T7036 agent by,determined
R4411 T7038 T7039 compound Bio,rad
R4412 T7039 T7041 compound rad,Assay
R4413 T7040 T7039 punct -,rad
R4414 T7041 T7037 pobj Assay,by
R4415 T7042 T7041 compound Dc,Assay
R4416 T7043 T7041 compound Protein,Assay
R4417 T7044 T7027 punct ),separated
R4418 T7045 T7027 auxpass was,separated
R4419 T7046 T7027 prep by,separated
R4420 T7047 T7048 compound SDS,PAGE
R4421 T7048 T7046 pobj PAGE,by
R4422 T7049 T7048 punct -,PAGE
R4423 T7050 T7027 punct ", ",separated
R4424 T7051 T7027 prep for,separated
R4425 T7052 T7053 amod subsequent,immunoblotting
R4426 T7053 T7051 pobj immunoblotting,for
R4427 T7054 T7027 punct ", ",separated
R4428 T7055 T7056 mark as,described
R4429 T7056 T7027 advcl described,separated
R4430 T7057 T7058 punct [,12
R4431 T7058 T7027 parataxis 12,separated
R4432 T7059 T7058 punct ],12
R4433 T7060 T7027 punct .,separated
R4434 T7062 T7063 nummod 16.5,%
R4435 T7063 T7064 compound %,gels
R4436 T7064 T7071 nsubjpass gels,used
R4437 T7065 T7066 compound tris,tricine
R4438 T7066 T7064 compound tricine,gels
R4439 T7067 T7066 punct -,tricine
R4440 T7068 T7069 compound SDS,PAGE
R4441 T7069 T7064 compound PAGE,gels
R4442 T7070 T7069 punct -,PAGE
R4443 T7072 T7071 auxpass were,used
R4444 T7073 T7071 prep for,used
R4445 T7074 T7075 compound subunit,c
R4446 T7075 T7076 compound c,experiments
R4447 T7076 T7073 pobj experiments,for
R4448 T7077 T7076 compound immunoblotting,experiments
R4449 T7078 T7071 punct .,used
R4453 T7121 T7122 nummod 100,mm
R4454 T7122 T7123 compound mm,dishes
R4455 T7123 T7126 nsubjpass dishes,washed
R4456 T7124 T7125 compound tissue,culture
R4457 T7125 T7123 compound culture,dishes
R4458 T7127 T7123 punct ", ",dishes
R4459 T7128 T7129 dep which,were
R4460 T7129 T7123 relcl were,dishes
R4461 T7130 T7131 advmod approximately,90
R4462 T7131 T7134 nummod 90,%
R4463 T7132 T7131 quantmod 80,90
R4464 T7133 T7131 punct –,90
R4465 T7134 T7135 npadvmod %,confluent
R4466 T7135 T7129 acomp confluent,were
R4467 T7136 T7126 punct ", ",washed
R4468 T7137 T7126 auxpass were,washed
R4469 T7138 T7126 advmod briefly,washed
R4470 T7139 T7126 prep with,washed
R4471 T7140 T7141 npadvmod ice,cold
R4472 T7141 T7143 amod cold,PBS
R4473 T7142 T7141 punct -,cold
R4474 T7143 T7139 pobj PBS,with
R4475 T7144 T7126 punct ", ",washed
R4476 T7145 T7126 cc and,washed
R4477 T7146 T7147 amod total,extracts
R4478 T7147 T7149 nsubjpass extracts,isolated
R4479 T7148 T7147 compound protein,extracts
R4480 T7149 T7126 conj isolated,washed
R4481 T7150 T7149 auxpass were,isolated
R4482 T7151 T7149 prep by,isolated
R4483 T7152 T7151 pcomp scraping,by
R4484 T7153 T7152 dobj cells,scraping
R4485 T7154 T7152 prep into,scraping
R4486 T7155 T7156 nummod 10,mM
R4487 T7156 T7157 compound mM,Tris
R4488 T7157 T7154 pobj Tris,into
R4489 T7158 T7157 punct ", ",Tris
R4490 T7159 T7157 npadvmod pH,Tris
R4491 T7160 T7159 nummod 7.4,pH
R4512 T7183 T7182 auxpass was,centrifuged
R4513 T7184 T7182 prep at,centrifuged
R4514 T7185 T7186 nummod "14,000",g
R4515 T7186 T7184 pobj g,at
R4516 T7187 T7182 punct ", ",centrifuged
R4517 T7188 T7189 det the,supernatant
R4518 T7189 T7190 nsubjpass supernatant,isolated
R4520 T7191 T7190 auxpass was,isolated
R4523 T7194 T7195 compound protein,concentration
R4524 T7195 T7196 nsubjpass concentration,determined
R4525 T7196 T7190 conj determined,isolated
R4526 T7197 T7196 auxpass was,determined
R4527 T7198 T7196 advcl using,determined
R4528 T7199 T7200 det the,Assay
R4529 T7200 T7198 dobj Assay,using
R4530 T7201 T7202 compound Bio,rad
R4531 T7202 T7200 compound rad,Assay
R4532 T7203 T7202 punct -,rad
R4533 T7204 T7200 compound Dc,Assay
R4534 T7205 T7200 compound Protein,Assay
R4535 T7206 T7196 punct .,determined
R4536 T7208 T7209 quantmod 50,70
R4537 T7209 T7211 nummod 70,μg
R4538 T7210 T7209 punct –,70
R4539 T7211 T7212 nsubjpass μg,used
R4540 T7213 T7211 prep of,μg
R4541 T7214 T7215 amod total,extract
R4542 T7215 T7213 pobj extract,of
R4543 T7216 T7215 compound protein,extract
R4544 T7217 T7212 auxpass were,used
R4545 T7218 T7219 aux to,measure
R4546 T7219 T7212 advcl measure,used
R4547 T7220 T7221 compound cathepsin,D
R4548 T7221 T7222 compound D,activity
R4549 T7222 T7219 dobj activity,measure
R4550 T7223 T7219 advcl using,measure
R4551 T7224 T7225 det the,Kit
R4552 T7225 T7223 dobj Kit,using
R4553 T7226 T7227 amod Fluorogenic,D
R4554 T7227 T7225 compound D,Kit
R4555 T7228 T7227 nmod Innozyme,D
R4556 T7229 T7227 punct ™,D
R4557 T7230 T7227 compound Cathepsin,D
R4558 T7231 T7225 compound Immunocapture,Kit
R4559 T7232 T7225 compound Activity,Kit
R4560 T7233 T7225 compound Assay,Kit
R4561 T7234 T7235 punct (,Biosciences
R4562 T7235 T7225 parataxis Biosciences,Kit
R4563 T7236 T7235 compound EMD,Biosciences
R4564 T7237 T7235 punct ),Biosciences
R4565 T7238 T7223 prep according,using
R4566 T7239 T7238 prep to,according
R4567 T7240 T7241 det the,manufacturer
R4568 T7241 T7242 poss manufacturer,recommendations
R4569 T7242 T7239 pobj recommendations,to
R4570 T7243 T7241 case 's,manufacturer
R4571 T7244 T7212 punct .,used
R4572 T7246 T7247 amod Relative,fluorescence
R4573 T7247 T7248 nsubjpass fluorescence,measured
R4574 T7249 T7248 auxpass was,measured
R4575 T7250 T7248 advcl using,measured
R4576 T7251 T7252 det an,reader
R4577 T7252 T7250 dobj reader,using
R4578 T7253 T7254 compound Analyst,AD
R4579 T7254 T7252 compound AD,reader
R4580 T7255 T7252 compound plate,reader
R4581 T7256 T7257 punct (,Devices
R4582 T7257 T7252 parataxis Devices,reader
R4583 T7258 T7257 amod Molecular,Devices
R4584 T7259 T7257 punct ),Devices
R4585 T7260 T7250 prep with,using
R4586 T7261 T7262 det the,filters
R4587 T7262 T7260 pobj filters,with
R4588 T7263 T7262 amod following,filters
R4589 T7264 T7262 cc and,filters
R4590 T7265 T7262 conj settings,filters
R4591 T7266 T7248 punct : ,measured
R4592 T7267 T7268 compound excitation,filter
R4593 T7268 T7269 dep filter,35
R4602 T7277 T7276 punct ", ",20
R4603 T7278 T7276 nummod 400,20
R4604 T7279 T7276 punct -,20
R4605 T7280 T7276 punct ", ",20
R4606 T7281 T7282 compound Flash,lamp
R4607 T7282 T7276 conj lamp,20
R4608 T7283 T7282 prep with,lamp
R4609 T7284 T7285 nummod 100,readings
R4610 T7285 T7283 pobj readings,with
R4611 T7286 T7287 punct /,well
R4612 T7287 T7285 prep well,readings
R4613 T7288 T7282 punct ", ",lamp
R4614 T7289 T7290 nummod 100,ms
R4615 T7290 T7282 conj ms,lamp
R4616 T7291 T7290 prep between,ms
R4617 T7292 T7291 pobj readings,between
R4618 T7293 T7290 punct ", ",ms
R4619 T7294 T7290 cc and,ms
R4620 T7295 T7296 nummod "100,000",μs
R4621 T7296 T7297 compound μs,time
R4622 T7297 T7290 conj time,ms
R4623 T7298 T7297 compound integration,time
R4624 T7299 T7248 punct .,measured
R4625 T7337 T7338 amod Lysosomal,staining
R4626 T7339 T7338 cc and,staining
R4627 T7340 T7341 amod endocytic,uptake
R4628 T7341 T7338 conj uptake,staining
R4629 T7343 T7344 nsubjpass Cells,seeded
R4630 T7345 T7344 auxpass were,seeded
R4631 T7346 T7344 prep at,seeded
R4632 T7347 T7348 det a,density
R4633 T7348 T7346 pobj density,at
R4634 T7349 T7348 prep of,density
R4635 T7350 T7351 quantmod 3,5
R4636 T7351 T7353 quantmod 5,104
R4637 T7352 T7351 punct –,5
R4638 T7353 T7355 nummod 104,cells
R4639 T7354 T7353 punct ×,104
R4640 T7355 T7349 pobj cells,of
R4641 T7356 T7355 prep per,cells
R4642 T7357 T7356 pobj well,per
R4643 T7358 T7344 prep in,seeded
R4644 T7359 T7360 nummod 4,well
R4645 T7360 T7362 compound well,slides
R4646 T7361 T7360 punct -,well
R4647 T7362 T7358 pobj slides,in
R4648 T7363 T7362 compound chamber,slides
R4649 T7364 T7362 punct -,slides
R4650 T7365 T7344 cc and,seeded
R4651 T7366 T7344 conj grown,seeded
R4652 T7367 T7366 advmod overnight,grown
R4653 T7368 T7366 prep at,grown
R4654 T7369 T7370 nummod 33,°C
R4655 T7370 T7368 pobj °C,at
R4656 T7371 T7344 punct .,seeded
R4657 T7373 T7374 compound Growth,media
R4658 T7374 T7375 nsubjpass media,exchanged
R4659 T7376 T7375 auxpass was,exchanged
R4660 T7377 T7375 prep for,exchanged
R4661 T7378 T7379 amod fresh,media
R4662 T7379 T7377 pobj media,for
R4663 T7380 T7379 punct ", ",media
R4664 T7381 T7379 amod pre-warmed,media
R4665 T7382 T7379 compound growth,media
R4666 T7383 T7379 acl containing,media
R4667 T7384 T7385 nummod 500,nM
R4668 T7385 T7386 compound nM,Lysotracker
R4669 T7386 T7383 dobj Lysotracker,containing
R4670 T7387 T7386 cc or,Lysotracker
R4671 T7388 T7389 nummod 1,mg
R4672 T7389 T7390 nmod mg,FITC
R4673 T7390 T7386 conj FITC,Lysotracker
R4674 T7391 T7392 punct /,ml
R4675 T7392 T7389 prep ml,mg
R4676 T7393 T7390 compound dextran,FITC
R4677 T7394 T7390 punct -,FITC
R4678 T7395 T7375 punct ", ",exchanged
R4679 T7396 T7375 cc and,exchanged
R4680 T7397 T7398 nsubjpass cells,incubated
R4681 T7398 T7375 conj incubated,exchanged
R4682 T7399 T7398 auxpass were,incubated
R4683 T7400 T7398 prep at,incubated
R4684 T7401 T7402 nummod 33,°C
R4685 T7402 T7400 pobj °C,at
R4686 T7403 T7398 prep for,incubated
R4687 T7404 T7405 nummod 45,minutes
R4688 T7405 T7403 pobj minutes,for
R4689 T7406 T7405 cc or,minutes
R4690 T7407 T7408 nummod 15,minutes
R4691 T7408 T7405 conj minutes,minutes
R4692 T7409 T7398 punct ", ",incubated
R4693 T7410 T7398 advmod respectively,incubated
R4694 T7411 T7398 punct .,incubated
R4695 T7413 T7414 prep Following,placed
R4696 T7415 T7413 pobj labeling,Following
R4697 T7416 T7414 punct ", ",placed
R4698 T7417 T7414 nsubjpass cells,placed
R4699 T7418 T7414 auxpass were,placed
R4700 T7419 T7414 advmod immediately,placed
R4701 T7420 T7414 prep on,placed
R4702 T7421 T7420 pobj ice,on
R4703 T7422 T7414 cc and,placed
R4704 T7423 T7414 conj washed,placed
R4705 T7424 T7423 prep for,washed
R4706 T7425 T7426 nummod 10,minutes
R4707 T7426 T7424 pobj minutes,for
R4708 T7427 T7423 prep in,washed
R4709 T7428 T7429 npadvmod ice,cold
R4710 T7429 T7431 amod cold,media
R4711 T7430 T7429 punct -,cold
R4712 T7431 T7427 pobj media,in
R4713 T7432 T7433 npadvmod dye,free
R4714 T7433 T7431 amod free,media
R4715 T7434 T7433 punct -,free
R4716 T7435 T7423 punct ", ",washed
R4717 T7436 T7423 cc and,washed
R4798 T7536 T7537 quantmod 2.5,4
R4718 T7437 T7423 conj fixed,washed
R4719 T7438 T7437 prep with,fixed
R4720 T7439 T7440 nummod 4,%
R4721 T7440 T7441 compound %,formaldehyde
R4722 T7441 T7438 pobj formaldehyde,with
R4723 T7442 T7441 prep in,formaldehyde
R4724 T7443 T7442 pobj PBS,in
R4725 T7444 T7437 punct ", ",fixed
R4726 T7445 T7437 prep for,fixed
R4727 T7446 T7447 nummod 20,minutes
R4728 T7447 T7445 pobj minutes,for
R4729 T7448 T7437 prep on,fixed
R4730 T7449 T7448 pobj ice,on
R4731 T7450 T7414 punct .,placed
R4732 T7452 T7453 nsubjpass Chambers,removed
R4733 T7454 T7453 auxpass were,removed
R4734 T7455 T7453 cc and,removed
R4735 T7456 T7457 nsubjpass slides,coverslipped
R4736 T7457 T7453 conj coverslipped,removed
R4737 T7458 T7457 auxpass were,coverslipped
R4738 T7459 T7457 prep with,coverslipped
R4739 T7460 T7461 compound Vectashield,media
R4740 T7461 T7459 pobj media,with
R4741 T7462 T7461 compound mounting,media
R4742 T7463 T7461 prep for,media
R4743 T7464 T7465 amod confocal,microscopy
R4744 T7465 T7466 compound microscopy,analysis
R4745 T7466 T7463 pobj analysis,for
R4746 T7467 T7457 punct ", ",coverslipped
R4747 T7468 T7469 mark as,described
R4748 T7469 T7457 advcl described,coverslipped
R4749 T7470 T7469 advmod above,described
R4750 T7471 T7457 punct .,coverslipped
R4751 T7484 T7485 amod Morphometric,analysis
R4752 T7486 T7485 prep of,analysis
R4753 T7487 T7486 pobj mitochondria,of
R4754 T7489 T7490 compound TEM,photomicrographs
R4755 T7490 T7491 nsubjpass photomicrographs,taken
R4756 T7492 T7493 punct (,magnification
R4757 T7493 T7490 parataxis magnification,photomicrographs
R4758 T7494 T7495 quantmod "10,000","40,000"
R4759 T7495 T7493 nummod "40,000",magnification
R4760 T7496 T7494 punct ×,"10,000"
R4761 T7497 T7495 punct –,"40,000"
R4762 T7498 T7495 punct ×,"40,000"
R4763 T7499 T7493 punct ),magnification
R4764 T7500 T7491 auxpass were,taken
R4765 T7501 T7491 prep from,taken
R4766 T7502 T7503 amod random,fields
R4767 T7503 T7501 pobj fields,from
R4768 T7504 T7503 compound grid,fields
R4769 T7505 T7491 punct .,taken
R4770 T7507 T7508 prep For,measured
R4771 T7509 T7510 compound length,measurements
R4772 T7510 T7507 pobj measurements,For
R4773 T7511 T7508 punct ", ",measured
R4774 T7512 T7513 det the,side
R4775 T7513 T7508 nsubjpass side,measured
R4776 T7514 T7513 amod longest,side
R4777 T7515 T7513 prep of,side
R4778 T7516 T7517 det each,mitochondria
R4779 T7517 T7515 pobj mitochondria,of
R4780 T7518 T7508 auxpass was,measured
R4781 T7519 T7508 prep in,measured
R4782 T7520 T7519 pobj centimeters,in
R4783 T7521 T7508 punct ", ",measured
R4784 T7522 T7508 cc and,measured
R4785 T7523 T7524 prep along,taken
R4786 T7524 T7508 conj taken,measured
R4787 T7525 T7526 det the,length
R4788 T7526 T7523 pobj length,along
R4789 T7527 T7526 prep of,length
R4790 T7528 T7529 det the,mitochondria
R4791 T7529 T7527 pobj mitochondria,of
R4792 T7530 T7524 punct ", ",taken
R4793 T7531 T7532 compound width,measurements
R4794 T7532 T7524 nsubjpass measurements,taken
R4795 T7533 T7524 auxpass were,taken
R4796 T7534 T7535 det every,mm
R4797 T7535 T7524 npadvmod mm,taken
R4799 T7537 T7535 nummod 4,mm
R4800 T7538 T7537 punct –,4
R4801 T7539 T7535 punct (,mm
R4802 T7540 T7535 amod dependent,mm
R4803 T7541 T7540 prep on,dependent
R4804 T7542 T7543 det the,magnification
R4805 T7543 T7541 pobj magnification,on
R4806 T7544 T7543 prep of,magnification
R4807 T7545 T7546 det the,image
R4808 T7546 T7544 pobj image,of
R4809 T7547 T7546 compound micrograph,image
R4810 T7548 T7524 punct ),taken
R4811 T7549 T7524 punct .,taken
R4812 T7551 T7552 prep Following,normalized
R4813 T7553 T7551 pobj measurement,Following
R4814 T7554 T7552 punct ", ",normalized
R4815 T7555 T7556 det all,numbers
R4816 T7556 T7552 nsubjpass numbers,normalized
R4817 T7557 T7552 auxpass were,normalized
R4818 T7558 T7559 aux to,reflect
R4819 T7559 T7552 advcl reflect,normalized
R4820 T7560 T7561 nummod one,magnification
R4821 T7561 T7559 dobj magnification,reflect
R4822 T7562 T7552 cc and,normalized
R4823 T7563 T7564 nsubjpass data,analyzed
R4824 T7564 T7552 conj analyzed,normalized
R4825 T7565 T7564 auxpass was,analyzed
R4826 T7566 T7564 advcl using,analyzed
R4827 T7567 T7568 compound Microsoft,Excel
R4828 T7568 T7569 compound Excel,software
R4829 T7569 T7566 dobj software,using
R4830 T7570 T7564 punct .,analyzed
R4831 T7572 T7573 aux To,ascertain
R4832 T7573 T7574 advcl ascertain,converted
R4833 T7575 T7576 amod unmagnified,size
R4834 T7576 T7573 dobj size,ascertain
R4835 T7577 T7576 amod mitochondrial,size
R4836 T7578 T7574 punct ", ",converted
R4837 T7579 T7580 amod final,data
R4838 T7580 T7574 nsubjpass data,converted
R4839 T7581 T7580 compound measurement,data
R4840 T7582 T7580 punct ", ",data
R4841 T7583 T7580 prep in,data
R4842 T7584 T7583 pobj centimeters,in
R4843 T7585 T7574 punct ", ",converted
R4844 T7586 T7574 auxpass was,converted
R4845 T7587 T7574 prep to,converted
R4846 T7588 T7587 pobj nanometers,to
R4847 T7589 T7574 prep according,converted
R4848 T7590 T7589 prep to,according
R4849 T7591 T7592 compound scale,bar
R4850 T7592 T7593 compound bar,representation
R4851 T7593 T7590 pobj representation,to
R4852 T7594 T7574 punct .,converted
R4853 T7619 T7620 compound ATP,measurement
R4854 T7622 T7623 nsubjpass ATP,measured
R4855 T7624 T7623 auxpass was,measured
R4856 T7625 T7623 prep by,measured
R4857 T7626 T7625 pcomp using,by
R4858 T7627 T7628 det the,kit
R4859 T7628 T7626 dobj kit,using
R4860 T7629 T7630 nmod CellTiter,GLO®
R4861 T7630 T7628 nmod GLO®,kit
R4862 T7631 T7630 punct -,GLO®
R4863 T7632 T7628 amod Luminescent,kit
R4864 T7633 T7634 compound Cell,Viability
R4865 T7634 T7628 compound Viability,kit
R4866 T7635 T7636 punct (,Promega
R4867 T7636 T7628 parataxis Promega,kit
R4868 T7637 T7636 punct ),Promega
R4869 T7638 T7623 punct ", ",measured
R4870 T7639 T7623 prep according,measured
R4871 T7640 T7639 prep to,according
R4872 T7641 T7642 det the,manufacturer
R4873 T7642 T7643 poss manufacturer,recommendations
R4874 T7643 T7640 pobj recommendations,to
R4875 T7644 T7642 case 's,manufacturer
R4876 T7645 T7623 punct .,measured
R4877 T7647 T7648 advmod Briefly,plated
R4878 T7649 T7648 punct ", ",plated
R4879 T7650 T7648 nsubjpass cells,plated
R4880 T7651 T7648 auxpass were,plated
R4881 T7652 T7648 prep in,plated
R4882 T7653 T7654 det a,plate
R4883 T7654 T7652 pobj plate,in
R4884 T7655 T7654 amod black,plate
R4885 T7656 T7657 amod opaque,walled
R4886 T7657 T7654 amod walled,plate
R4887 T7658 T7657 punct -,walled
R4888 T7659 T7660 nummod 96,well
R4889 T7660 T7654 compound well,plate
R4890 T7661 T7662 punct (,Bioscience
R4891 T7662 T7654 parataxis Bioscience,plate
R4892 T7663 T7662 compound Packard,Bioscience
R4893 T7664 T7662 punct ),Bioscience
R4894 T7665 T7648 prep at,plated
R4895 T7666 T7667 det a,density
R4896 T7667 T7665 pobj density,at
R4897 T7668 T7667 prep of,density
R4898 T7669 T7668 pobj 20000,of
R4899 T7670 T7671 punct /,well
R4900 T7671 T7669 prep well,20000
R4901 T7672 T7648 cc and,plated
R4902 T7673 T7648 conj incubated,plated
R4903 T7674 T7673 prep at,incubated
R4904 T7675 T7676 nummod 33,°C
R4905 T7676 T7674 pobj °C,at
R4906 T7677 T7673 advmod overnight,incubated
R4907 T7678 T7648 punct .,plated
R4908 T7680 T7681 det The,day
R4909 T7681 T7683 npadvmod day,added
R4910 T7682 T7681 amod following,day
R4911 T7684 T7683 punct ", ",added
R4912 T7685 T7686 compound CellTiter,GLO®
R4913 T7686 T7688 compound GLO®,Reagent
R4914 T7687 T7686 punct -,GLO®
R4915 T7688 T7683 nsubjpass Reagent,added
R4916 T7689 T7683 auxpass was,added
R4917 T7690 T7683 prep to,added
R4918 T7691 T7692 det each,well
R4919 T7692 T7690 pobj well,to
R4920 T7693 T7683 cc and,added
R4921 T7694 T7695 compound cell,lysis
R4922 T7695 T7696 nsubjpass lysis,induced
R4923 T7696 T7683 conj induced,added
R4924 T7697 T7696 auxpass was,induced
R4925 T7698 T7696 prep by,induced
R4926 T7699 T7698 pcomp mixing,by
R4927 T7700 T7701 nummod 2,minutes
R4928 T7701 T7699 npadvmod minutes,mixing
R4929 T7702 T7696 punct .,induced
R4930 T7704 T7705 det An,curve
R4931 T7705 T7708 nsubjpass curve,prepared
R4932 T7706 T7705 nmod ATP,curve
R4933 T7707 T7705 amod standard,curve
R4934 T7709 T7708 auxpass was,prepared
R4935 T7710 T7708 prep in,prepared
R4936 T7711 T7712 det the,plate
R4937 T7712 T7710 pobj plate,in
R4938 T7713 T7712 amod same,plate
R4939 T7714 T7708 punct .,prepared
R4940 T7716 T7717 prep Before,adapted
R4941 T7718 T7716 pcomp recording,Before
R4942 T7719 T7718 dobj luminescence,recording
R4943 T7720 T7718 prep with,recording
R4944 T7721 T7722 det a,luminometer
R4945 T7722 T7720 pobj luminometer,with
R4946 T7723 T7722 compound microplate,luminometer
R4947 T7724 T7722 punct (,luminometer
R4948 T7725 T7726 compound MicroLumat,96V
R4949 T7726 T7722 appos 96V,luminometer
R4950 T7727 T7726 compound Plus,96V
R4951 T7728 T7726 compound LB,96V
R4952 T7729 T7730 punct ", ",Techonologies
R4953 T7730 T7722 parataxis Techonologies,luminometer
R4954 T7731 T7730 compound Berthold,Techonologies
R4955 T7732 T7730 punct ),Techonologies
R4956 T7733 T7717 punct ", ",adapted
R4957 T7734 T7735 det the,plate
R4958 T7735 T7717 nsubjpass plate,adapted
R4959 T7736 T7717 auxpass was,adapted
R4960 T7737 T7717 dep dark,adapted
R4961 T7738 T7717 prep for,adapted
R4962 T7739 T7740 nummod 10,minutes
R4963 T7740 T7738 pobj minutes,for
R4964 T7741 T7717 prep at,adapted
R4965 T7742 T7743 compound room,temperature
R4966 T7743 T7741 pobj temperature,at
R4967 T7744 T7745 aux to,stabilize
R4968 T7745 T7717 advcl stabilize,adapted
R4969 T7746 T7747 det the,signal
R4970 T7747 T7745 dobj signal,stabilize
R4971 T7748 T7747 compound luminescence,signal
R4972 T7749 T7717 punct .,adapted
R4973 T7780 T7781 compound Hydrogen,peroxide
R4974 T7781 T7782 compound peroxide,treatment
R4975 T7782 T7783 compound treatment,assay
R4976 T7785 T7786 nsubjpass Cells,plated
R4977 T7787 T7786 auxpass were,plated
R4978 T7788 T7786 prep at,plated
R4979 T7789 T7790 det a,density
R4980 T7790 T7788 pobj density,at
R4981 T7791 T7790 prep of,density
R4982 T7792 T7793 nummod "10,000",cells
R4983 T7793 T7791 pobj cells,of
R4984 T7794 T7795 punct /,well
R4985 T7795 T7793 prep well,cells
R4986 T7796 T7786 prep in,plated
R4987 T7797 T7798 nummod 96,well
R4988 T7798 T7800 compound well,plates
R4989 T7799 T7798 punct -,well
R4990 T7800 T7796 pobj plates,in
R4991 T7801 T7786 cc and,plated
R4992 T7802 T7786 conj incubated,plated
R4993 T7803 T7802 prep at,incubated
R4994 T7804 T7805 nummod 33,°C
R4995 T7805 T7803 pobj °C,at
R4996 T7806 T7802 advmod overnight,incubated
R4997 T7807 T7786 punct .,plated
R4998 T7809 T7810 det The,day
R4999 T7810 T7812 npadvmod day,dispersed
R5000 T7811 T7810 amod following,day
R5001 T7813 T7812 punct ", ",dispersed
R5002 T7814 T7815 amod fresh,media
R5003 T7815 T7812 nsubjpass media,dispersed
R5004 T7816 T7815 acl containing,media
R5005 T7817 T7818 amod varying,concentrations
R5006 T7818 T7816 dobj concentrations,containing
R5007 T7819 T7818 prep of,concentrations
R5008 T7820 T7821 compound hydrogen,peroxide
R5009 T7821 T7819 pobj peroxide,of
R5010 T7822 T7812 auxpass was,dispersed
R5011 T7823 T7812 prep to,dispersed
R5012 T7824 T7825 det each,well
R5013 T7825 T7823 pobj well,to
R5014 T7826 T7812 punct .,dispersed
R5015 T7828 T7829 nsubjpass Cells,incubated
R5016 T7830 T7829 auxpass were,incubated
R5017 T7831 T7829 prep in,incubated
R5018 T7832 T7833 det the,presence
R5019 T7833 T7831 pobj presence,in
R5020 T7834 T7833 prep of,presence
R5021 T7835 T7836 compound hydrogen,peroxide
R5022 T7836 T7834 pobj peroxide,of
R5023 T7837 T7829 prep for,incubated
R5024 T7838 T7839 nummod 24,hours
R5025 T7839 T7837 pobj hours,for
R5026 T7840 T7829 punct ", ",incubated
R5027 T7841 T7829 prep at,incubated
R5028 T7842 T7843 nummod 33,°C
R5029 T7843 T7841 pobj °C,at
R5030 T7844 T7829 punct ", ",incubated
R5031 T7845 T7829 cc and,incubated
R5032 T7846 T7847 nsubjpass viability,measured
R5033 T7847 T7829 conj measured,incubated
R5034 T7848 T7847 auxpass was,measured
R5035 T7849 T7847 advcl using,measured
R5036 T7850 T7851 det the,Assay
R5037 T7851 T7849 dobj Assay,using
R5038 T7852 T7853 nmod CellTiter,96®
R5039 T7853 T7851 nmod 96®,Assay
R5040 T7854 T7853 punct -,96®
R5041 T7855 T7851 amod AQueous,Assay
R5042 T7856 T7851 amod Non-Radioactive,Assay
R5043 T7857 T7851 compound Cell,Assay
R5044 T7858 T7851 compound Proliferation,Assay
R5045 T7859 T7860 punct (,Promega
R5046 T7860 T7851 parataxis Promega,Assay
R5047 T7861 T7860 punct ),Promega
R5048 T7862 T7849 punct ", ",using
R5049 T7863 T7849 prep according,using
R5050 T7864 T7863 prep to,according
R5051 T7865 T7866 det the,manufacturer
R5052 T7866 T7867 poss manufacturer,specifications
R5053 T7867 T7864 pobj specifications,to
R5054 T7868 T7866 case 's,manufacturer
R5055 T7869 T7847 punct .,measured
R5063 T7990 T7987 punct /,CbCln3
R5064 T7991 T7987 punct +,CbCln3
R5065 T7992 T7985 nsubjpass Characterization,shown
R5066 T7993 T7992 prep of,Characterization
R5067 T7994 T7995 nmod CbCln3,cells
R5068 T7995 T7993 pobj cells,of
R5069 T7996 T7994 punct +,CbCln3
R5070 T7997 T7994 punct /,CbCln3
R5071 T7998 T7994 punct +,CbCln3
R5072 T7999 T7992 prep by,Characterization
R5073 T8000 T7999 pobj immunofluorescence,by
R5074 T8001 T8000 prep with,immunofluorescence
R5075 T8002 T8003 compound marker,antibodies
R5076 T8003 T8001 pobj antibodies,with
R5077 T8004 T7985 auxpass is,shown
R5078 T8005 T7985 punct .,shown
R5079 T8007 T8008 nmod CbCln3,precursors
R5080 T8008 T8012 nsubj precursors,exhibit
R5081 T8009 T8007 punct +,CbCln3
R5082 T8010 T8007 punct /,CbCln3
R5083 T8011 T8007 punct +,CbCln3
R5084 T8013 T8014 compound nestin,expression
R5085 T8014 T8012 dobj expression,exhibit
R5086 T8015 T8016 punct (,a
R5087 T8016 T8014 parataxis a,expression
R5088 T8017 T8016 punct ),a
R5089 T8018 T8014 cc but,expression
R5090 T8019 T8018 neg not,but
R5091 T8020 T8021 compound GFAP,expression
R5092 T8021 T8014 conj expression,expression
R5093 T8022 T8023 punct (,b
R5094 T8023 T8021 parataxis b,expression
R5095 T8024 T8023 punct ),b
R5096 T8025 T8012 punct ", ",exhibit
R5097 T8026 T8012 advcl consistent,exhibit
R5098 T8027 T8026 prep with,consistent
R5099 T8028 T8029 det a,identity
R5100 T8029 T8027 pobj identity,with
R5101 T8030 T8029 amod neuronal,identity
R5102 T8031 T8029 compound precursor,identity
R5103 T8032 T8012 punct .,exhibit
R5104 T8034 T8035 prep Upon,achieved
R5105 T8036 T8034 pobj stimulation,Upon
R5106 T8037 T8036 prep with,stimulation
R5107 T8038 T8039 det a,cocktail
R5108 T8039 T8037 pobj cocktail,with
R5109 T8040 T8039 compound differentiation,cocktail
R5110 T8041 T8042 punct (,see
R5111 T8042 T8036 parataxis see,stimulation
R5112 T8043 T8042 dobj Methods,see
R5113 T8044 T8042 punct ),see
R5114 T8045 T8035 punct ", ",achieved
R5115 T8046 T8047 nmod CbCln3,cells
R5117 T8048 T8046 punct +,CbCln3
R5120 T8051 T8052 npadvmod neuron,like
R5121 T8052 T8054 amod like,morphology
R5122 T8053 T8052 punct -,like
R5123 T8054 T8035 dobj morphology,achieved
R5124 T8055 T8035 punct ", ",achieved
R5125 T8056 T8035 prep with,achieved
R5126 T8057 T8058 amod rounded,bodies
R5127 T8058 T8056 pobj bodies,with
R5128 T8059 T8058 compound cell,bodies
R5129 T8060 T8058 cc and,bodies
R5130 T8061 T8058 conj extension,bodies
R5131 T8062 T8061 prep of,extension
R5132 T8063 T8062 pobj processes,of
R5133 T8064 T8035 punct ", ",achieved
R5134 T8065 T8035 cc and,achieved
R5135 T8066 T8067 nmod MAP2,expression
R5136 T8067 T8076 nsubjpass expression,increased
R5137 T8068 T8069 punct (,c
R5138 T8069 T8066 parataxis c,MAP2
R5139 T8070 T8069 punct ),c
R5140 T8071 T8066 cc and,MAP2
R5141 T8072 T8066 conj NeuN,MAP2
R5142 T8073 T8074 punct (,d
R5143 T8074 T8072 parataxis d,NeuN
R5144 T8075 T8074 punct ),d
R5145 T8076 T8035 conj increased,achieved
R5146 T8077 T8076 auxpass was,increased
R5147 T8078 T8076 punct .,increased
R5148 T8080 T8081 nmod CbCln3,cells
R5149 T8081 T8085 nsubj cells,are
R5150 T8082 T8080 punct +,CbCln3
R5151 T8083 T8080 punct /,CbCln3
R5152 T8084 T8080 punct +,CbCln3
R5153 T8086 T8085 acomp negative,are
R5154 T8087 T8086 prep for,negative
R5155 T8088 T8089 det the,marker
R5156 T8089 T8087 pobj marker,for
R5157 T8090 T8089 compound Purkinje,marker
R5158 T8091 T8089 compound neuron,marker
R5159 T8092 T8089 appos calbindin,marker
R5160 T8093 T8094 punct (,e
R5161 T8094 T8089 parataxis e,marker
R5162 T8095 T8094 punct ),e
R5163 T8096 T8085 punct .,are
R5164 T8098 T8099 nmod CbCln3,Δex7
R5165 T8099 T8102 nmod Δex7,lines
R5166 T8100 T8099 punct +,Δex7
R5167 T8101 T8099 punct /,Δex7
R5168 T8102 T8114 nsubj lines,exhibited
R5169 T8103 T8099 punct /,Δex7
R5170 T8104 T8099 nummod 8,Δex7
R5171 T8105 T8099 cc and,Δex7
R5172 T8106 T8107 nmod CbCln3Δex7,Δex7
R5173 T8107 T8099 conj Δex7,Δex7
R5174 T8108 T8107 punct /,Δex7
R5175 T8109 T8107 nummod 8,Δex7
R5176 T8110 T8107 punct /,Δex7
R5177 T8111 T8107 punct /,Δex7
R5178 T8112 T8107 nummod 8,Δex7
R5179 T8113 T8102 compound cell,lines
R5180 T8115 T8116 amod identical,results
R5181 T8116 T8114 dobj results,exhibited
R5182 T8117 T8118 compound marker,immunofluorescence
R5183 T8118 T8116 compound immunofluorescence,results
R5184 T8119 T8114 punct .,exhibited
R5185 T8121 T8122 meta a,magnification
R5186 T8123 T8121 punct ", ",a
R5187 T8124 T8121 dep b,a
R5188 T8125 T8121 punct ),a
R5189 T8126 T8122 nummod 20,magnification
R5190 T8127 T8126 punct ×,20
R5191 T8128 T8122 punct ;,magnification
R5195 T8132 T8129 dep d,c
R5196 T8133 T8129 punct ", ",c
R5197 T8134 T8129 dep e,c
R5198 T8135 T8129 punct ),c
R5199 T8136 T8130 nummod 40,magnification
R5200 T8137 T8136 punct ×,40
R5201 T8138 T8122 punct .,magnification
R5202 T8215 T8216 compound RT,PCR
R5203 T8216 T8218 dep PCR,shown
R5204 T8217 T8216 punct -,PCR
R5205 T8219 T8216 prep of,PCR
R5206 T8220 T8221 compound Cln3,mRNA
R5207 T8221 T8219 pobj mRNA,of
R5208 T8222 T8216 prep in,PCR
R5209 T8223 T8224 amod wild,type
R5210 T8224 T8226 nmod type,cells
R5211 T8225 T8224 punct -,type
R5212 T8226 T8222 pobj cells,in
R5213 T8227 T8224 cc and,type
R5214 T8228 T8224 conj homozygous,type
R5215 T8229 T8226 nmod CbCln3Δex7,cells
R5216 T8230 T8229 punct /,CbCln3Δex7
R5217 T8231 T8229 nummod 8,CbCln3Δex7
R5218 T8232 T8233 nmod Cln3,products
R5219 T8233 T8218 nsubjpass products,shown
R5220 T8234 T8235 npadvmod Exon1,forward
R5221 T8235 T8237 amod forward,reverse
R5222 T8236 T8235 punct -,forward
R5223 T8237 T8233 nmod reverse,products
R5224 T8238 T8237 punct ", ",reverse
R5225 T8239 T8237 npadvmod Exon,reverse
R5226 T8240 T8239 nummod 15,Exon
R5227 T8241 T8237 punct -,reverse
R5228 T8242 T8243 compound RT,PCR
R5229 T8243 T8233 compound PCR,products
R5230 T8244 T8243 punct -,PCR
R5231 T8245 T8218 auxpass are,shown
R5232 T8246 T8218 punct ", ",shown
R5233 T8247 T8218 prep from,shown
R5234 T8248 T8249 amod total,RNA
R5235 T8249 T8247 pobj RNA,from
R5236 T8250 T8251 amod wild,type
R5237 T8251 T8249 nmod type,RNA
R5238 T8252 T8251 punct -,type
R5239 T8253 T8251 punct (,type
R5240 T8254 T8255 punct +,+
R5241 T8255 T8251 appos +,type
R5242 T8256 T8255 punct /,+
R5243 T8257 T8251 punct ),type
R5244 T8258 T8251 cc or,type
R5245 T8259 T8260 amod homozygous,mutant
R5246 T8260 T8251 conj mutant,type
R5247 T8261 T8260 punct (,mutant
R5248 T8262 T8260 appos Δex7,mutant
R5249 T8263 T8262 punct /,Δex7
R5250 T8264 T8262 nummod 8,Δex7
R5251 T8265 T8262 punct /,Δex7
R5252 T8266 T8262 appos Δex7,Δex7
R5253 T8267 T8266 punct /,Δex7
R5254 T8268 T8266 nummod 8,Δex7
R5255 T8269 T8249 punct ),RNA
R5256 T8270 T8249 nmod brain,RNA
R5257 T8271 T8270 cc and,brain
R5258 T8272 T8273 compound cell,line
R5259 T8273 T8270 conj line,brain
R5260 T8274 T8218 punct .,shown
R5261 T8276 T8277 nmod Brain,products
R5262 T8277 T8285 nsubj products,had
R5263 T8278 T8276 cc and,Brain
R5264 T8279 T8280 compound cell,line
R5265 T8280 T8276 conj line,Brain
R5266 T8281 T8282 compound RT,PCR
R5267 T8282 T8277 compound PCR,products
R5268 T8283 T8282 punct -,PCR
R5269 T8284 T8277 compound reaction,products
R5270 T8286 T8287 amod identical,patterns
R5271 T8287 T8285 dobj patterns,had
R5272 T8288 T8287 compound band,patterns
R5273 T8289 T8285 prep on,had
R5274 T8290 T8291 compound ethidium,bromide
R5275 T8291 T8293 npadvmod bromide,stained
R5276 T8292 T8291 punct -,bromide
R5277 T8293 T8294 amod stained,gels
R5278 T8294 T8289 pobj gels,on
R5279 T8295 T8294 compound agarose,gels
R5280 T8296 T8285 punct .,had
R5281 T8298 T8299 amod Wild,type
R5282 T8299 T8301 compound type,product
R5283 T8300 T8299 punct -,type
R5284 T8301 T8305 nsubj product,was
R5285 T8302 T8303 compound RT,PCR
R5286 T8303 T8301 compound PCR,product
R5287 T8304 T8303 punct -,PCR
R5288 T8306 T8307 det a,band
R5289 T8307 T8305 attr band,was
R5290 T8308 T8307 amod single,band
R5291 T8309 T8310 punct ~,1.6
R5292 T8310 T8311 nummod 1.6,kb
R5293 T8311 T8307 compound kb,band
R5294 T8312 T8305 cc and,was
R5295 T8313 T8314 compound mutant,products
R5296 T8314 T8315 nsubj products,were
R5297 T8315 T8305 conj were,was
R5298 T8316 T8317 punct ~,1.6
R5299 T8317 T8318 nummod 1.6,kb
R5300 T8318 T8315 attr kb,were
R5301 T8319 T8317 punct ", ",1.6
R5302 T8320 T8321 punct ~,1.5
R5303 T8321 T8317 conj 1.5,1.6
R5304 T8322 T8321 punct ", ",1.5
R5305 T8323 T8324 punct ~,1.4
R5306 T8324 T8321 conj 1.4,1.5
R5307 T8325 T8324 punct ", ",1.4
R5308 T8326 T8327 punct ~,1.35
R5309 T8327 T8324 conj 1.35,1.4
R5310 T8328 T8327 punct ", ",1.35
R5311 T8329 T8327 cc and,1.35
R5312 T8330 T8331 punct ~,1.3
R5313 T8331 T8327 conj 1.3,1.35
R5314 T8332 T8315 punct ", ",were
R5315 T8333 T8315 advcl representing,were
R5316 T8334 T8335 amod multiple,variants
R5317 T8335 T8333 dobj variants,representing
R5318 T8336 T8337 compound mutant,splice
R5319 T8337 T8335 compound splice,variants
R5320 T8338 T8315 punct .,were
R5343 T8468 T8469 amod wild,type
R5344 T8469 T8467 pobj type,of
R5345 T8470 T8469 punct -,type
R5346 T8471 T8469 punct (,type
R5347 T8472 T8469 appos CbCln3,type
R5348 T8473 T8472 punct +,CbCln3
R5349 T8474 T8472 punct /,CbCln3
R5350 T8475 T8472 punct +,CbCln3
R5351 T8476 T8469 punct ),type
R5352 T8477 T8469 cc and,type
R5353 T8478 T8479 amod homozygous,mutant
R5354 T8479 T8480 nmod mutant,cells
R5356 T8481 T8479 punct (,mutant
R5357 T8482 T8483 nmod CbCln3Δex7,Δex7
R5358 T8483 T8479 appos Δex7,mutant
R5359 T8484 T8483 punct /,Δex7
R5360 T8485 T8483 nummod 8,Δex7
R5361 T8486 T8483 punct /,Δex7
R5362 T8487 T8483 punct /,Δex7
R5363 T8488 T8483 nummod 8,Δex7
R5364 T8489 T8480 punct ),cells
R5365 T8490 T8480 amod cerebellar,cells
R5366 T8491 T8480 compound precursor,cells
R5367 T8492 T8452 auxpass is,shown
R5368 T8493 T8452 punct ", ",shown
R5369 T8494 T8452 prep with,shown
R5370 T8495 T8494 pobj co-staining,with
R5371 T8496 T8495 prep for,co-staining
R5372 T8497 T8496 pobj lysosomes,for
R5373 T8498 T8497 punct (,lysosomes
R5374 T8499 T8497 appos Lamp,lysosomes
R5375 T8500 T8499 nummod 1,Lamp
R5376 T8501 T8497 punct ),lysosomes
R5377 T8502 T8497 punct ", ",lysosomes
R5378 T8503 T8504 amod early,endosomes
R5379 T8504 T8497 conj endosomes,lysosomes
R5380 T8505 T8504 punct (,endosomes
R5381 T8506 T8504 appos EEA1,endosomes
R5382 T8507 T8504 punct ),endosomes
R5383 T8508 T8504 punct ", ",endosomes
R5384 T8509 T8504 cc and,endosomes
R5385 T8510 T8511 amod late,endosomes
R5386 T8511 T8504 conj endosomes,endosomes
R5387 T8512 T8511 punct (,endosomes
R5388 T8513 T8511 appos Rab7,endosomes
R5389 T8514 T8452 punct ),shown
R5390 T8515 T8452 punct .,shown
R5391 T8517 T8518 amod Significant,overlap
R5392 T8518 T8519 nsubjpass overlap,seen
R5393 T8520 T8518 prep of,overlap
R5394 T8521 T8522 compound Batp1,signal
R5395 T8522 T8520 pobj signal,of
R5396 T8523 T8524 punct (,red
R5397 T8524 T8522 parataxis red,signal
R5398 T8525 T8524 punct ),red
R5399 T8526 T8518 prep with,overlap
R5400 T8527 T8526 pobj EEA1,with
R5401 T8528 T8529 punct (,panels
R5402 T8529 T8527 parataxis panels,EEA1
R5403 T8530 T8529 amod green,panels
R5404 T8531 T8529 punct ", ",panels
R5405 T8532 T8529 amod middle,panels
R5406 T8533 T8529 punct ),panels
R5407 T8534 T8527 cc and,EEA1
R5408 T8535 T8527 conj Rab7,EEA1
R5409 T8536 T8537 punct (,panels
R5410 T8537 T8535 parataxis panels,Rab7
R5411 T8538 T8537 amod green,panels
R5412 T8539 T8537 punct ", ",panels
R5413 T8540 T8537 amod bottom,panels
R5414 T8541 T8537 punct ),panels
R5415 T8542 T8519 aux can,seen
R5416 T8543 T8519 auxpass be,seen
R5417 T8544 T8519 prep as,seen
R5418 T8545 T8544 amod yellow,as
R5419 T8546 T8547 advmod when,merged
R5420 T8547 T8519 advcl merged,seen
R5421 T8548 T8549 det the,channels
R5422 T8549 T8547 nsubjpass channels,merged
R5423 T8550 T8549 nummod two,channels
R5424 T8551 T8547 auxpass are,merged
R5425 T8552 T8553 punct (,Merge
R5426 T8553 T8547 parataxis Merge,merged
R5427 T8554 T8553 punct ),Merge
R5428 T8555 T8519 punct .,seen
R5429 T8557 T8558 det The,degree
R5430 T8558 T8559 nsubj degree,is
R5431 T8560 T8558 prep of,degree
R5432 T8561 T8562 compound Batp1,overlap
R5433 T8562 T8560 pobj overlap,of
R5434 T8563 T8559 acomp greatest,is
R5435 T8564 T8559 prep with,is
R5436 T8565 T8564 pobj Rab7,with
R5437 T8566 T8559 punct .,is
R5438 T8568 T8569 advmod Only,overlap
R5439 T8569 T8571 nsubjpass overlap,seen
R5440 T8570 T8569 amod limited,overlap
R5441 T8572 T8569 prep between,overlap
R5442 T8573 T8572 pobj Batp1,between
R5443 T8574 T8575 punct (,red
R5444 T8575 T8573 parataxis red,Batp1
R5445 T8576 T8575 punct ),red
R5446 T8577 T8573 cc and,Batp1
R5447 T8578 T8573 conj Lamp,Batp1
R5448 T8579 T8578 nummod 1,Lamp
R5449 T8580 T8581 punct (,panels
R5450 T8581 T8578 parataxis panels,Lamp
R5451 T8582 T8581 amod green,panels
R5452 T8583 T8581 punct ", ",panels
R5453 T8584 T8581 amod top,panels
R5454 T8585 T8581 punct ),panels
R5455 T8586 T8571 aux can,seen
R5456 T8587 T8571 auxpass be,seen
R5457 T8588 T8571 punct .,seen
R5458 T8590 T8591 compound Batp1,signal
R5459 T8591 T8592 nsubj signal,is
R5460 T8593 T8591 prep in,signal
R5461 T8594 T8595 amod homozygous,cells
R5462 T8595 T8593 pobj cells,in
R5463 T8596 T8595 nmod CbCln3Δex7,cells
R5464 T8597 T8596 punct /,CbCln3Δex7
R5465 T8598 T8596 nummod 8,CbCln3Δex7
R5466 T8599 T8600 advmod significantly,reduced
R5467 T8600 T8592 acomp reduced,is
R5468 T8601 T8592 punct ", ",is
R5469 T8602 T8592 cc but,is
R5470 T8603 T8604 amod significant,overlap
R5471 T8604 T8605 nsubjpass overlap,seen
R5472 T8605 T8592 conj seen,is
R5473 T8606 T8604 prep with,overlap
R5474 T8607 T8606 pobj EEA1,with
R5475 T8608 T8607 cc and,EEA1
R5476 T8609 T8607 conj Rab7,EEA1
R5477 T8610 T8604 punct ", ",overlap
R5478 T8611 T8604 cc and,overlap
R5479 T8612 T8613 advmod very,little
R5480 T8613 T8614 amod little,overlap
R5481 T8614 T8604 conj overlap,overlap
R5482 T8615 T8614 nmod Lamp,overlap
R5483 T8616 T8615 nummod 1,Lamp
R5484 T8617 T8605 punct ", ",seen
R5485 T8618 T8605 aux can,seen
R5486 T8619 T8605 auxpass be,seen
R5487 T8620 T8605 prep as,seen
R5488 T8621 T8620 amod yellow,as
R5489 T8622 T8605 prep in,seen
R5490 T8623 T8624 det the,panels
R5491 T8624 T8622 pobj panels,in
R5492 T8625 T8624 amod respective,panels
R5493 T8626 T8624 amod merged,panels
R5494 T8627 T8605 punct .,seen
R5495 T8629 T8630 advmod Notably,appear
R5496 T8631 T8630 punct ", ",appear
R5497 T8632 T8633 nmod Lamp,localization
R5498 T8633 T8630 nsubj localization,appear
R5499 T8634 T8632 nummod 1,Lamp
R5500 T8635 T8632 cc and,Lamp
R5501 T8636 T8632 conj EEA1,Lamp
R5502 T8637 T8630 oprd altered,appear
R5503 T8638 T8630 punct ", ",appear
R5504 T8639 T8630 cc and,appear
R5537 T8805 T8806 compound Subunit,c
R5538 T8806 T8807 compound c,accumulation
R5539 T8808 T8807 prep in,accumulation
R5540 T8809 T8810 amod homozygous,cells
R5541 T8810 T8808 pobj cells,in
R5542 T8811 T8810 nmod CbCln3Δex7,cells
R5543 T8812 T8811 punct /,CbCln3Δex7
R5544 T8813 T8811 nummod 8,CbCln3Δex7
R5545 T8814 T8815 amod cerebellar,precursor
R5546 T8815 T8810 compound precursor,cells
R5547 T8817 T8818 meta a,shown
R5548 T8819 T8817 punct .,a
R5549 T8820 T8821 compound Subunit,c
R5550 T8821 T8818 nsubjpass c,shown
R5551 T8822 T8821 appos immunostaining,c
R5552 T8823 T8822 cc and,immunostaining
R5553 T8824 T8822 conj autofluorescence,immunostaining
R5554 T8825 T8821 prep of,c
R5555 T8826 T8827 nummod 7,day
R5556 T8827 T8829 compound day,confluency
R5557 T8828 T8827 punct -,day
R5558 T8829 T8830 npadvmod confluency,aged
R5559 T8830 T8832 amod aged,cells
R5560 T8831 T8830 punct -,aged
R5561 T8832 T8825 pobj cells,of
R5562 T8833 T8834 amod wild,type
R5563 T8834 T8832 nmod type,cells
R5564 T8835 T8834 punct -,type
R5565 T8836 T8834 cc and,type
R5566 T8837 T8834 conj homozygous,type
R5567 T8838 T8832 nmod CbCln3Δex7,cells
R5568 T8839 T8838 punct /,CbCln3Δex7
R5569 T8840 T8838 nummod 8,CbCln3Δex7
R5570 T8841 T8818 auxpass is,shown
R5571 T8842 T8818 punct .,shown
R5572 T8844 T8845 amod Wild,type
R5573 T8845 T8847 compound type,cultures
R5574 T8846 T8845 punct -,type
R5575 T8847 T8848 nsubj cultures,exhibited
R5576 T8849 T8847 punct (,cultures
R5577 T8850 T8847 appos CbCln3,cultures
R5578 T8851 T8850 punct +,CbCln3
R5579 T8852 T8850 punct /,CbCln3
R5580 T8853 T8850 punct +,CbCln3
R5581 T8854 T8848 punct ),exhibited
R5582 T8855 T8856 amod limited,c
R5583 T8856 T8848 dobj c,exhibited
R5584 T8857 T8856 compound subunit,c
R5585 T8858 T8856 appos immunostaining,c
R5586 T8859 T8858 cc and,immunostaining
R5587 T8860 T8858 conj autofluorescence,immunostaining
R5588 T8861 T8848 punct .,exhibited
R5589 T8863 T8864 advmod However,contained
R5590 T8865 T8864 punct ", ",contained
R5591 T8866 T8867 nmod CbCln3Δex7,Δex7
R5592 T8867 T8871 nmod Δex7,cells
R5593 T8868 T8867 punct /,Δex7
R5594 T8869 T8867 nummod 8,Δex7
R5595 T8870 T8867 punct /,Δex7
R5596 T8871 T8864 nsubj cells,contained
R5597 T8872 T8867 punct /,Δex7
R5598 T8873 T8867 nummod 8,Δex7
R5599 T8874 T8875 amod numerous,puncta
R5600 T8875 T8864 dobj puncta,contained
R5601 T8876 T8877 compound subunit,c
R5602 T8877 T8875 compound c,puncta
R5603 T8878 T8864 punct .,contained
R5604 T8880 T8881 nsubj Autofluorescence,was
R5605 T8882 T8883 punct (,AF
R5606 T8883 T8880 parataxis AF,Autofluorescence
R5607 T8884 T8885 nummod 7,days
R5608 T8885 T8883 compound days,AF
R5609 T8886 T8883 punct ),AF
R5610 T8887 T8881 advmod also,was
R5611 T8888 T8889 advmod significantly,elevated
R5612 T8889 T8881 acomp elevated,was
R5613 T8890 T8891 punct (,panels
R5614 T8891 T8889 parataxis panels,elevated
R5615 T8892 T8891 amod right,panels
R5616 T8893 T8891 punct ),panels
R5617 T8894 T8881 punct ", ",was
R5618 T8895 T8896 mark although,observed
R5619 T8896 T8881 advcl observed,was
R5620 T8897 T8898 amod limited,overlap
R5621 T8898 T8896 nsubjpass overlap,observed
R5622 T8899 T8898 prep with,overlap
R5623 T8900 T8901 compound subunit,c
R5624 T8901 T8902 compound c,puncta
R5625 T8902 T8899 pobj puncta,with
R5626 T8903 T8896 auxpass was,observed
R5627 T8904 T8905 punct (,arrows
R5628 T8905 T8896 parataxis arrows,observed
R5629 T8906 T8905 punct ),arrows
R5630 T8907 T8881 punct .,was
R5631 T8909 T8910 nummod 40,magnification
R5632 T8911 T8909 punct ×,40
R5633 T8912 T8910 punct .,magnification
R5634 T8914 T8915 meta b,shown
R5635 T8916 T8914 punct .,b
R5636 T8917 T8918 compound Immunoblot,analysis
R5637 T8918 T8915 nsubjpass analysis,shown
R5638 T8919 T8918 prep of,analysis
R5639 T8920 T8921 compound subunit,c
R5640 T8921 T8922 compound c,protein
R5641 T8922 T8919 pobj protein,of
R5642 T8923 T8918 prep at,analysis
R5643 T8924 T8925 nmod sub-confluency,incubation
R5644 T8925 T8923 pobj incubation,at
R5645 T8926 T8924 cc or,sub-confluency
R5646 T8927 T8928 nummod 7,day
R5647 T8928 T8930 compound day,confluency
R5648 T8929 T8928 punct -,day
R5649 T8930 T8924 conj confluency,sub-confluency
R5650 T8931 T8915 auxpass is,shown
R5651 T8932 T8915 punct .,shown
R5652 T8934 T8935 amod Total,extracts
R5653 T8935 T8937 nsubj extracts,contained
R5654 T8936 T8935 compound protein,extracts
R5655 T8938 T8935 prep from,extracts
R5656 T8939 T8940 nmod sub-confluency,cultures
R5657 T8940 T8938 pobj cultures,from
R5658 T8941 T8942 amod wild,type
R5659 T8942 T8940 nmod type,cultures
R5660 T8943 T8942 punct -,type
R5661 T8944 T8942 punct (,type
R5662 T8945 T8946 punct +,+
R5663 T8946 T8942 appos +,type
R5664 T8947 T8946 punct /,+
R5665 T8948 T8942 punct ),type
R5666 T8949 T8942 cc and,type
R5667 T8950 T8951 amod homozygous,mutant
R5668 T8951 T8942 conj mutant,type
R5669 T8952 T8951 punct (,mutant
R5670 T8953 T8951 appos Δex7,mutant
R5671 T8954 T8953 punct /,Δex7
R5672 T8955 T8953 nummod 8,Δex7
R5673 T8956 T8953 punct /,Δex7
R5674 T8957 T8953 appos Δex7,Δex7
R5675 T8958 T8957 punct /,Δex7
R5676 T8959 T8957 nummod 8,Δex7
R5677 T8960 T8940 punct ),cultures
R5678 T8961 T8962 advmod approximately,equal
R5679 T8962 T8963 amod equal,levels
R5680 T8963 T8937 dobj levels,contained
R5681 T8964 T8963 prep of,levels
R5682 T8965 T8966 compound subunit,c
R5683 T8966 T8967 compound c,protein
R5684 T8967 T8964 pobj protein,of
R5685 T8968 T8967 punct (,protein
R5686 T8969 T8970 nmod α,c
R5687 T8970 T8967 appos c,protein
R5688 T8971 T8969 punct -,α
R5689 T8972 T8969 amod sub,α
R5690 T8973 T8937 punct ),contained
R5691 T8974 T8937 punct .,contained
R5692 T8976 T8977 nummod 7,day
R5693 T8977 T8979 compound day,confluency
R5694 T8978 T8977 punct -,day
R5695 T8979 T8980 compound confluency,extract
R5696 T8980 T8981 nsubj extract,had
R5697 T8982 T8980 prep from,extract
R5698 T8983 T8984 amod homozygous,cells
R5699 T8984 T8982 pobj cells,from
R5700 T8985 T8984 nmod CbCln3Δex7,cells
R5701 T8986 T8985 punct /,CbCln3Δex7
R5702 T8987 T8985 nummod 8,CbCln3Δex7
R5703 T8988 T8984 punct (,cells
R5704 T8989 T8984 appos Δex7,cells
R5705 T8990 T8989 punct /,Δex7
R5706 T8991 T8989 nummod 8,Δex7
R5707 T8992 T8989 punct /,Δex7
R5708 T8993 T8989 appos Δex7,Δex7
R5709 T8994 T8993 punct /,Δex7
R5710 T8995 T8993 nummod 8,Δex7
R5711 T8996 T8981 punct ),had
R5712 T8997 T8998 amod elevated,levels
R5713 T8998 T8981 dobj levels,had
R5714 T8999 T8998 prep of,levels
R5715 T9000 T9001 compound subunit,c
R5716 T9001 T9002 compound c,protein
R5717 T9002 T8999 pobj protein,of
R5718 T9003 T8998 punct (,levels
R5719 T9004 T9005 punct ~,1.5
R5720 T9005 T8998 npadvmod 1.5,levels
R5721 T9006 T9005 punct X,1.5
R5722 T9007 T8998 punct ),levels
R5723 T9008 T8998 punct ", ",levels
R5724 T9009 T8998 amod relative,levels
R5725 T9010 T9009 prep to,relative
R5726 T9011 T9012 amod wild,type
R5727 T9012 T9014 compound type,extract
R5728 T9013 T9012 punct -,type
R5729 T9014 T9010 pobj extract,to
R5730 T9015 T9014 punct (,extract
R5731 T9016 T9017 punct +,+
R5732 T9017 T9014 appos +,extract
R5733 T9018 T9017 punct /,+
R5734 T9019 T8981 punct ),had
R5735 T9020 T8981 punct .,had
R5736 T9022 T9023 compound Protein,levels
R5737 T9023 T9024 nsubjpass levels,normalized
R5738 T9025 T9024 auxpass were,normalized
R5739 T9026 T9024 prep to,normalized
R5740 T9027 T9028 compound cytochrome,c
R5741 T9028 T9029 compound c,oxidase
R5742 T9029 T9030 compound oxidase,subunit
R5743 T9030 T9026 pobj subunit,to
R5744 T9031 T9030 nummod IV,subunit
R5745 T9032 T9030 punct (,subunit
R5746 T9033 T9034 compound α,cox4
R5747 T9034 T9030 appos cox4,subunit
R5748 T9035 T9034 punct -,cox4
R5749 T9036 T9024 punct ),normalized
R5750 T9037 T9024 punct .,normalized
R5751 T9039 T9040 meta c,shown
R5752 T9041 T9039 punct .,c
R5753 T9042 T9043 compound TEM,analysis
R5754 T9043 T9040 nsubjpass analysis,shown
R5755 T9044 T9043 prep of,analysis
R5756 T9045 T9044 pobj inclusions,of
R5757 T9046 T9043 prep in,analysis
R5758 T9047 T9048 nummod 7,day
R5759 T9048 T9050 compound day,confluency
R5760 T9049 T9048 punct -,day
R5761 T9050 T9051 npadvmod confluency,aged
R5762 T9051 T9053 amod aged,cells
R5763 T9052 T9051 punct -,aged
R5764 T9053 T9046 pobj cells,in
R5765 T9054 T9053 amod homozygous,cells
R5766 T9055 T9053 nmod CbCln3Δex7,cells
R5767 T9056 T9055 punct /,CbCln3Δex7
R5768 T9057 T9055 nummod 8,CbCln3Δex7
R5769 T9058 T9040 auxpass is,shown
R5770 T9059 T9040 punct .,shown
R5771 T9061 T9062 det A,autophagosome
R5772 T9062 T9064 nsubjpass autophagosome,filled
R5773 T9063 T9062 amod large,autophagosome
R5774 T9065 T9062 acl contained,autophagosome
R5775 T9066 T9065 agent by,contained
R5776 T9067 T9068 amod double,membrane
R5777 T9068 T9066 pobj membrane,by
R5778 T9069 T9070 punct (,arrows
R5779 T9070 T9065 parataxis arrows,contained
R5780 T9071 T9070 punct ),arrows
R5781 T9072 T9064 auxpass is,filled
R5782 T9073 T9064 prep with,filled
R5783 T9074 T9075 amod degenerating,mitochondria
R5784 T9075 T9073 pobj mitochondria,with
R5785 T9076 T9075 punct (,mitochondria
R5786 T9077 T9075 appos Md,mitochondria
R5787 T9078 T9075 punct ),mitochondria
R5788 T9079 T9075 punct ", ",mitochondria
R5789 T9080 T9081 nmod electron,cores
R5790 T9081 T9075 conj cores,mitochondria
R5791 T9082 T9081 amod dense,cores
R5792 T9083 T9084 punct (,left
R5793 T9084 T9081 parataxis left,cores
R5794 T9085 T9084 cc and,left
R5795 T9086 T9084 conj right,left
R5796 T9087 T9084 prep of,left
R5797 T9088 T9087 punct *,of
R5798 T9089 T9084 punct ),left
R5799 T9090 T9081 cc and,cores
R5800 T9091 T9092 amod other,structures
R5801 T9092 T9081 conj structures,cores
R5802 T9093 T9092 amod smaller,structures
R5803 T9094 T9092 amod vesicular,structures
R5804 T9095 T9064 punct .,filled
R5805 T9097 T9098 det A,inclusion
R5806 T9098 T9103 nsubj inclusion,is
R5807 T9099 T9098 amod large,inclusion
R5808 T9100 T9101 npadvmod electron,dense
R5809 T9101 T9098 amod dense,inclusion
R5810 T9102 T9101 punct -,dense
R5811 T9104 T9098 punct ", ",inclusion
R5812 T9105 T9098 prep with,inclusion
R5813 T9106 T9107 det a,appearance
R5814 T9107 T9105 pobj appearance,with
R5815 T9108 T9107 nmod lipofuscin,appearance
R5816 T9109 T9108 punct (,lipofuscin
R5817 T9110 T9108 appos Ln,lipofuscin
R5818 T9111 T9107 punct ),appearance
R5819 T9112 T9103 punct ", ",is
R5820 T9113 T9103 advmod also,is
R5821 T9114 T9103 acomp present,is
R5822 T9115 T9103 punct .,is
R5823 T9118 T9117 punct ", ",M
R5824 T9119 T9117 appos mitochondria,M
R5825 T9120 T9117 punct .,M
R5826 T9122 T9123 nummod "10,000",magnification
R5827 T9124 T9122 punct ×,"10,000"
R5828 T9125 T9123 punct .,magnification
R5834 T9260 T9261 amod wild,type
R5835 T9261 T9263 nmod type,cells
R5836 T9262 T9261 punct -,type
R5838 T9264 T9261 cc and,type
R5839 T9265 T9261 conj homozygous,type
R5840 T9266 T9263 nmod CbCln3Δex7,cells
R5841 T9267 T9266 punct /,CbCln3Δex7
R5862 T9290 T9273 acl recognizing,Immunostaining
R5863 T9291 T9292 amod unprocessed,forms
R5864 T9292 T9290 dobj forms,recognizing
R5865 T9293 T9291 cc and,unprocessed
R5866 T9294 T9291 conj processed,unprocessed
R5867 T9295 T9292 prep of,forms
R5868 T9296 T9297 compound cathepsin,D
R5869 T9297 T9298 compound D,protein
R5870 T9298 T9295 pobj protein,of
R5871 T9299 T9271 auxpass is,shown
R5872 T9300 T9271 punct .,shown
R5873 T9302 T9303 nmod CbCln3,cells
R5874 T9303 T9307 nsubj cells,exhibited
R5875 T9304 T9302 punct +,CbCln3
R5876 T9305 T9302 punct /,CbCln3
R5877 T9306 T9302 punct +,CbCln3
R5878 T9308 T9309 punct (,panel
R5879 T9309 T9303 parataxis panel,cells
R5880 T9310 T9309 amod left,panel
R5881 T9311 T9309 punct ),panel
R5882 T9312 T9313 det a,signal
R5883 T9313 T9307 dobj signal,exhibited
R5884 T9314 T9313 amod perinuclear,signal
R5885 T9315 T9314 cc and,perinuclear
R5886 T9316 T9314 conj cytoplasmic,perinuclear
R5887 T9317 T9313 amod punctate,signal
R5888 T9318 T9307 punct .,exhibited
R5889 T9320 T9321 compound Cathepsin,D
R5890 T9321 T9322 compound D,signal
R5891 T9322 T9323 nsubj signal,was
R5892 T9324 T9322 prep in,signal
R5893 T9325 T9326 amod homozygous,cells
R5894 T9326 T9324 pobj cells,in
R5895 T9327 T9326 nmod CbCln3Δex7,cells
R5896 T9328 T9327 punct /,CbCln3Δex7
R5897 T9329 T9327 nummod 8,CbCln3Δex7
R5898 T9330 T9331 punct (,panel
R5899 T9331 T9322 parataxis panel,signal
R5900 T9332 T9331 amod right,panel
R5901 T9333 T9331 punct ),panel
R5902 T9334 T9335 advmod more,often
R5903 T9335 T9323 advmod often,was
R5904 T9336 T9323 acomp perinuclear,was
R5905 T9337 T9323 punct ", ",was
R5906 T9338 T9323 prep with,was
R5907 T9339 T9340 advmod less,signal
R5908 T9340 T9338 pobj signal,with
R5909 T9341 T9342 amod cytoplasmic,punctate
R5910 T9342 T9340 compound punctate,signal
R5911 T9343 T9340 punct ", ",signal
R5912 T9344 T9340 prep compared,signal
R5913 T9345 T9344 prep to,compared
R5914 T9346 T9347 amod wild,type
R5915 T9347 T9349 nmod type,cells
R5916 T9348 T9347 punct -,type
R5917 T9349 T9345 pobj cells,to
R5918 T9350 T9349 nmod CbCln3,cells
R5919 T9351 T9350 punct +,CbCln3
R5920 T9352 T9350 punct /,CbCln3
R5921 T9353 T9350 punct +,CbCln3
R5922 T9354 T9323 punct .,was
R5923 T9356 T9357 nummod 40,magnification
R5924 T9358 T9356 punct ×,40
R5925 T9359 T9357 punct .,magnification
R5926 T9361 T9362 meta b,shown
R5927 T9363 T9361 punct .,b
R5928 T9364 T9365 compound α,D
R5929 T9365 T9368 npadvmod D,probed
R5930 T9366 T9365 punct -,D
R5931 T9367 T9365 compound Cathepsin,D
R5932 T9368 T9370 amod probed,immunoblots
R5933 T9369 T9368 punct -,probed
R5934 T9370 T9362 nsubjpass immunoblots,shown
R5935 T9371 T9370 prep of,immunoblots
R5936 T9372 T9373 amod total,tissue
R5938 T9374 T9375 amod wild,type
R5939 T9375 T9373 nmod type,tissue
R5940 T9376 T9375 punct -,type
R5959 T9396 T9397 punct ~,45
R5960 T9397 T9398 nummod 45,kDa
R5961 T9398 T9395 compound kDa,band
R5962 T9399 T9400 compound cathepsin,D
R5963 T9400 T9395 compound D,band
R5972 T9410 T9411 amod wild,type
R5973 T9411 T9409 pobj type,in
R5974 T9412 T9411 punct -,type
R5975 T9413 T9411 punct (,type
R5976 T9414 T9411 appos wt,type
R5977 T9415 T9411 punct ),type
R5978 T9416 T9411 appos tissue,type
R5979 T9417 T9416 cc and,tissue
R5980 T9418 T9419 amod cellular,extracts
R5981 T9419 T9416 conj extracts,tissue
R5982 T9420 T9407 punct ", ",band
R5983 T9421 T9407 prep with,band
R5984 T9422 T9423 amod lower,levels
R5985 T9423 T9421 pobj levels,with
R5986 T9424 T9423 prep of,levels
R5987 T9425 T9426 amod mature,enzyme
R5988 T9426 T9424 pobj enzyme,of
R5989 T9427 T9428 punct (,kDa
R5990 T9428 T9401 parataxis kDa,was
R5991 T9429 T9430 amod single,chain
R5992 T9430 T9428 dep chain,kDa
R5993 T9431 T9428 punct ", ",kDa
R5994 T9432 T9433 punct ~,43
R5995 T9433 T9428 nummod 43,kDa
R5996 T9434 T9428 punct ", ",kDa
R5997 T9435 T9428 cc and,kDa
R5998 T9436 T9437 amod heavy,chain
R5999 T9437 T9438 dep chain,kDa
R6000 T9438 T9428 conj kDa,kDa
R6001 T9439 T9438 punct ", ",kDa
R6002 T9440 T9441 punct ~,31
R6003 T9441 T9438 nummod 31,kDa
R6004 T9442 T9438 punct ),kDa
R6005 T9443 T9401 punct .,was
R6006 T9445 T9446 advmod Conversely,exhibited
R6007 T9447 T9446 punct ", ",exhibited
R6008 T9448 T9449 amod homozygous,extracts
R6009 T9449 T9446 nsubj extracts,exhibited
R6010 T9450 T9449 nmod Cln3Δex7,extracts
R6011 T9451 T9450 punct /,Cln3Δex7
R6012 T9452 T9450 nummod 8,Cln3Δex7
R6013 T9453 T9450 cc and,Cln3Δex7
R6014 T9454 T9450 conj CbCln3Δex7,Cln3Δex7
R6015 T9455 T9454 punct /,CbCln3Δex7
R6016 T9456 T9454 nummod 8,CbCln3Δex7
R6017 T9457 T9449 nmod mutant,extracts
R6018 T9458 T9457 punct (,mutant
R6019 T9459 T9457 appos m,mutant
R6020 T9460 T9449 punct ),extracts
R6021 T9461 T9462 amod reduced,levels
R6022 T9462 T9446 dobj levels,exhibited
R6023 T9463 T9462 prep of,levels
R6024 T9464 T9463 pobj precursor,of
R6025 T9465 T9464 cc and,precursor
R6026 T9466 T9467 amod heavy,chain
R6027 T9467 T9464 conj chain,precursor
R6028 T9468 T9464 prep of,precursor
R6029 T9469 T9470 det the,form
R6030 T9470 T9468 pobj form,of
R6031 T9471 T9472 amod double,chain
R6032 T9472 T9470 compound chain,form
R6033 T9473 T9472 punct -,chain
R6034 T9474 T9470 prep of,form
R6035 T9475 T9476 det the,enzyme
R6036 T9476 T9474 pobj enzyme,of
R6037 T9477 T9446 punct ", ",exhibited
R6038 T9478 T9446 prep with,exhibited
R6039 T9479 T9480 amod elevated,levels
R6040 T9480 T9478 pobj levels,with
R6041 T9481 T9480 prep of,levels
R6042 T9482 T9483 amod single,chain
R6043 T9483 T9485 nmod chain,enzyme
R6044 T9484 T9483 punct -,chain
R6045 T9485 T9481 pobj enzyme,of
R6046 T9486 T9485 amod mature,enzyme
R6047 T9487 T9446 punct .,exhibited
R6050 T9581 T9582 nmod Lysotracker,labeling
R6051 T9582 T9586 dep labeling,shown
R6052 T9583 T9581 cc and,Lysotracker
R6053 T9584 T9581 conj Lamp,Lysotracker
R6054 T9585 T9584 nummod 2,Lamp
R6055 T9587 T9582 prep of,labeling
R6056 T9588 T9589 amod wild,type
R6057 T9589 T9591 nmod type,lysosomes
R6058 T9590 T9589 punct -,type
R6060 T9592 T9589 cc and,type
R6090 T9624 T9623 amod top,panels
R6091 T9625 T9623 punct ),panels
R6092 T9626 T9627 amod large,lysosomes
R6093 T9627 T9621 dobj lysosomes,labeled
R6094 T9628 T9627 punct ", ",lysosomes
R6095 T9629 T9630 amod perinuclear,clustered
R6096 T9630 T9627 amod clustered,lysosomes
R6102 T9636 T9637 det the,periphery
R6108 T9642 T9638 pobj cells,of
R6109 T9643 T9642 punct (,cells
R6110 T9644 T9642 appos CbCln3,cells
R6111 T9645 T9644 punct +,CbCln3
R6112 T9646 T9644 punct /,CbCln3
R6113 T9647 T9644 punct +,CbCln3
R6114 T9648 T9621 punct ),labeled
R6115 T9649 T9621 punct .,labeled
R6116 T9651 T9652 compound Lysotracker,stain
R6117 T9652 T9653 nsubj stain,was
R6118 T9654 T9653 advmod dramatically,was
R6119 T9655 T9653 acomp reduced,was
R6120 T9656 T9653 prep in,was
R6121 T9657 T9658 amod homozygous,cells
R6122 T9658 T9656 pobj cells,in
R6123 T9659 T9658 compound mutant,cells
R6124 T9660 T9658 punct (,cells
R6125 T9661 T9658 appos CbCln3Δex7,cells
R6126 T9662 T9661 punct /,CbCln3Δex7
R6127 T9663 T9661 nummod 8,CbCln3Δex7
R6128 T9664 T9661 punct /,CbCln3Δex7
R6129 T9665 T9661 appos Δex7,CbCln3Δex7
R6130 T9666 T9665 punct /,Δex7
R6131 T9667 T9665 nummod 8,Δex7
R6132 T9668 T9653 punct ),was
R6133 T9669 T9653 punct ", ",was
R6134 T9670 T9653 prep with,was
R6135 T9671 T9672 amod smaller,vesicles
R6136 T9672 T9670 pobj vesicles,with
R6137 T9673 T9672 amod labeled,vesicles
R6138 T9674 T9672 cc and,vesicles
R6139 T9675 T9676 advmod less,apparent
R6140 T9676 T9677 amod apparent,clustering
R6141 T9677 T9672 conj clustering,vesicles
R6142 T9678 T9677 amod perinuclear,clustering
R6143 T9679 T9653 punct .,was
R6144 T9681 T9682 nmod Lamp,immunostaining
R6145 T9682 T9688 nsubj immunostaining,showed
R6146 T9683 T9681 nummod 2,Lamp
R6147 T9684 T9685 punct (,panels
R6148 T9685 T9681 parataxis panels,Lamp
R6149 T9686 T9685 amod bottom,panels
R6150 T9687 T9685 punct ),panels
R6151 T9689 T9688 advmod also,showed
R6152 T9690 T9691 amod reduced,intensity
R6153 T9691 T9688 dobj intensity,showed
R6154 T9692 T9691 compound signal,intensity
R6155 T9693 T9688 prep with,showed
R6159 T9697 T9688 prep in,showed
R6160 T9698 T9699 amod homozygous,cells
R6161 T9699 T9697 pobj cells,in
R6162 T9700 T9699 nmod CbCln3Δex7,cells
R6163 T9701 T9700 punct /,CbCln3Δex7
R6164 T9702 T9700 nummod 8,CbCln3Δex7
R6165 T9703 T9688 punct ", ",showed
R6166 T9704 T9705 mark although,was
R6167 T9705 T9688 advcl was,showed
R6168 T9706 T9707 det the,effect
R6169 T9707 T9705 nsubj effect,was
R6170 T9708 T9709 advmod somewhat,less
R6171 T9709 T9710 advmod less,dramatic
R6172 T9710 T9705 acomp dramatic,was
R6173 T9711 T9710 prep than,dramatic
R6174 T9712 T9711 pobj that,than
R6175 T9713 T9712 acl observed,that
R6176 T9714 T9713 prep with,observed
R6177 T9715 T9716 compound Lysotracker,dye
R6178 T9716 T9714 pobj dye,with
R6179 T9717 T9688 punct .,showed
R6180 T9719 T9720 amod Wild,type
R6181 T9720 T9722 nmod type,images
R6182 T9721 T9720 punct -,type
R6183 T9722 T9729 nsubjpass images,captured
R6184 T9723 T9720 cc and,type
R6185 T9724 T9720 conj homozygous,type
R6186 T9725 T9722 nmod CbCln3Δex7,images
R6187 T9726 T9725 punct /,CbCln3Δex7
R6188 T9727 T9725 nummod 8,CbCln3Δex7
R6189 T9728 T9722 amod confocal,images
R6190 T9730 T9729 auxpass were,captured
R6191 T9731 T9729 prep with,captured
R6192 T9732 T9733 amod identical,settings
R6193 T9733 T9731 pobj settings,with
R6194 T9734 T9733 compound exposure,settings
R6195 T9735 T9729 punct .,captured
R6196 T9737 T9738 nummod 60,magnification
R6197 T9739 T9737 punct ×,60
R6198 T9740 T9738 punct .,magnification
R6199 T9816 T9817 dep Endocytosis,shown
R6200 T9818 T9816 prep in,Endocytosis
R6201 T9819 T9820 amod wild,type
R6203 T9821 T9820 punct -,type
R6204 T9822 T9818 pobj cells,in
R6205 T9823 T9820 punct ", ",type
R6206 T9824 T9820 conj heterozygous,type
R6207 T9825 T9824 cc and,heterozygous
R6208 T9826 T9824 conj homozygous,heterozygous
R6209 T9827 T9822 nmod CbCln3Δex7,cells
R6210 T9828 T9827 punct /,CbCln3Δex7
R6211 T9829 T9827 nummod 8,CbCln3Δex7
R6212 T9830 T9831 compound Dextran,FITC
R6213 T9831 T9833 compound FITC,uptake
R6214 T9832 T9831 punct -,FITC
R6215 T9833 T9817 nsubjpass uptake,shown
R6216 T9834 T9833 prep in,uptake
R6217 T9835 T9836 amod wild,type
R6218 T9836 T9838 nmod type,cells
R6219 T9837 T9836 punct -,type
R6221 T9839 T9836 punct ", ",type
R6222 T9840 T9836 conj heterozygous,type
R6223 T9841 T9840 cc and,heterozygous
R6224 T9842 T9840 conj homozygous,heterozygous
R6235 T9855 T9850 pobj cells,In
R6248 T9868 T9866 parataxis panel,heterozygous
R6249 T9869 T9870 nmod CbCln3,Δex7
R6250 T9870 T9868 dep Δex7,panel
R6251 T9871 T9870 punct +,Δex7
R6252 T9872 T9870 punct /,Δex7
R6253 T9873 T9870 punct /,Δex7
R6254 T9874 T9870 nummod 8,Δex7
R6255 T9875 T9868 punct ", ",panel
R6256 T9876 T9868 amod middle,panel
R6257 T9877 T9868 punct ),panel
R6258 T9878 T9851 punct ", ",observed
R6259 T9879 T9880 compound dextran,FITC
R6260 T9880 T9882 compound FITC,label
R6261 T9881 T9880 punct -,FITC
R6262 T9882 T9851 nsubjpass label,observed
R6263 T9883 T9851 auxpass was,observed
R6264 T9884 T9851 prep in,observed
R6265 T9885 T9886 det a,pattern
R6266 T9886 T9884 pobj pattern,in
R6267 T9887 T9888 amod perinuclear,clustered
R6268 T9888 T9886 amod clustered,pattern
R6269 T9889 T9888 punct -,clustered
R6270 T9890 T9886 amod vesicular,pattern
R6271 T9891 T9851 prep with,observed
R6272 T9892 T9893 amod scattered,vesicles
R6274 T9894 T9893 amod labeled,vesicles
R6276 T9896 T9893 relcl present,vesicles
R6277 T9897 T9896 prep in,present
R6278 T9898 T9899 det the,periphery
R6279 T9899 T9897 pobj periphery,in
R6280 T9900 T9851 punct .,observed
R6281 T9902 T9903 prep In,reduced
R6282 T9904 T9902 pobj contrast,In
R6283 T9905 T9903 punct ", ",reduced
R6284 T9906 T9907 compound dextran,FITC
R6285 T9907 T9909 compound FITC,label
R6286 T9908 T9907 punct -,FITC
R6287 T9909 T9903 nsubjpass label,reduced
R6288 T9910 T9909 prep of,label
R6289 T9911 T9912 amod homozygous,mutant
R6290 T9912 T9913 nmod mutant,cells
R6291 T9913 T9910 pobj cells,of
R6292 T9914 T9912 punct (,mutant
R6293 T9915 T9916 nmod CbCln3Δex7,Δex7
R6294 T9916 T9912 appos Δex7,mutant
R6295 T9917 T9916 punct /,Δex7
R6296 T9918 T9916 nummod 8,Δex7
R6297 T9919 T9916 punct /,Δex7
R6298 T9920 T9916 punct /,Δex7
R6299 T9921 T9916 nummod 8,Δex7
R6300 T9922 T9923 punct ", ",panel
R6301 T9923 T9912 parataxis panel,mutant
R6302 T9924 T9923 amod right,panel
R6303 T9925 T9923 punct ),panel
R6304 T9926 T9903 auxpass was,reduced
R6305 T9927 T9903 advmod overall,reduced
R6306 T9928 T9903 cc and,reduced
R6307 T9929 T9903 conj exhibited,reduced
R6308 T9930 T9931 amod smaller,vesicles
R6309 T9931 T9929 dobj vesicles,exhibited
R6310 T9932 T9931 amod stained,vesicles
R6311 T9933 T9931 prep with,vesicles
R6312 T9934 T9935 advmod less,clustering
R6313 T9935 T9933 pobj clustering,with
R6314 T9936 T9935 amod perinuclear,clustering
R6315 T9937 T9903 punct .,reduced
R6316 T9939 T9940 amod Confocal,images
R6317 T9940 T9941 nsubjpass images,captured
R6318 T9942 T9941 auxpass were,captured
R6319 T9943 T9941 prep with,captured
R6320 T9944 T9945 amod identical,settings
R6321 T9945 T9943 pobj settings,with
R6322 T9946 T9945 compound exposure,settings
R6323 T9947 T9941 punct .,captured
R6324 T9949 T9950 nummod 40,magnification
R6325 T9951 T9949 punct ×,40
R6326 T9952 T9950 punct .,magnification
R6327 T10102 T10103 amod Mitochondrial,morphology
R6328 T10104 T10103 cc and,morphology
R6329 T10105 T10103 conj function,morphology
R6330 T10106 T10103 prep in,morphology
R6331 T10107 T10108 amod wild,type
R6332 T10108 T10110 nmod type,cells
R6333 T10109 T10108 punct -,type
R6334 T10110 T10106 pobj cells,in
R6335 T10111 T10108 punct ", ",type
R6336 T10112 T10108 conj heterozygous,type
R6337 T10113 T10112 cc and,heterozygous
R6338 T10114 T10112 conj homozygous,heterozygous
R6339 T10115 T10110 nmod CbCln3Δex7,cells
R6340 T10116 T10115 punct /,CbCln3Δex7
R6341 T10117 T10115 nummod 8,CbCln3Δex7
R6342 T10119 T10120 meta a,shown
R6343 T10121 T10119 punct .,a
R6344 T10122 T10123 amod Confocal,micrographs
R6345 T10123 T10120 nsubjpass micrographs,shown
R6346 T10124 T10122 cc and,Confocal
R6347 T10125 T10122 conj TEM,Confocal
R6348 T10126 T10123 prep of,micrographs
R6349 T10127 T10128 amod wild,type
R6350 T10128 T10130 nmod type,morphology
R6351 T10129 T10128 punct -,type
R6352 T10130 T10126 pobj morphology,of
R6353 T10131 T10128 cc and,type
R6354 T10132 T10128 conj homozygous,type
R6355 T10133 T10130 nmod CbCln3Δex7,morphology
R6356 T10134 T10133 punct /,CbCln3Δex7
R6357 T10135 T10133 nummod 8,CbCln3Δex7
R6358 T10136 T10130 amod mitochondrial,morphology
R6359 T10137 T10120 auxpass are,shown
R6360 T10138 T10120 punct .,shown
R6361 T10140 T10141 nsubj Immunostaining,highlighted
R6362 T10142 T10140 prep with,Immunostaining
R6363 T10143 T10144 det the,marker
R6364 T10144 T10142 pobj marker,with
R6365 T10145 T10144 amod inner,marker
R6366 T10146 T10144 amod mitochondrial,marker
R6367 T10147 T10144 compound membrane,marker
R6368 T10148 T10144 punct ", ",marker
R6369 T10149 T10144 appos grp75,marker
R6370 T10150 T10151 punct (,panels
R6371 T10151 T10140 parataxis panels,Immunostaining
R6372 T10152 T10151 amod top,panels
R6373 T10153 T10151 punct ),panels
R6374 T10154 T10155 amod elongated,mitochondria
R6375 T10155 T10141 dobj mitochondria,highlighted
R6376 T10156 T10141 prep in,highlighted
R6377 T10157 T10158 amod homozygous,cells
R6378 T10158 T10156 pobj cells,in
R6379 T10159 T10158 compound mutant,cells
R6380 T10160 T10158 punct (,cells
R6381 T10161 T10162 nmod CbCln3Δex7,Δex7
R6382 T10162 T10158 appos Δex7,cells
R6383 T10163 T10162 punct /,Δex7
R6384 T10164 T10162 nummod 8,Δex7
R6385 T10165 T10162 punct /,Δex7
R6386 T10166 T10162 punct /,Δex7
R6387 T10167 T10162 nummod 8,Δex7
R6388 T10168 T10141 punct ),highlighted
R6389 T10169 T10141 punct ", ",highlighted
R6390 T10170 T10141 advcl relative,highlighted
R6391 T10171 T10170 prep to,relative
R6392 T10172 T10173 amod wild,type
R6393 T10173 T10175 compound type,mitochondria
R6394 T10174 T10173 punct -,type
R6395 T10175 T10171 pobj mitochondria,to
R6396 T10176 T10175 punct (,mitochondria
R6397 T10177 T10175 appos CbCln3,mitochondria
R6398 T10178 T10177 punct +,CbCln3
R6399 T10179 T10177 punct /,CbCln3
R6400 T10180 T10177 punct +,CbCln3
R6401 T10181 T10170 punct ),relative
R6402 T10182 T10183 punct (,insets
R6403 T10183 T10170 parataxis insets,relative
R6404 T10184 T10183 punct ", ",insets
R6405 T10185 T10186 nsubj zoom,2.75
R6406 T10186 T10183 ccomp 2.75,insets
R6407 T10187 T10186 punct =,2.75
R6412 T10193 T10194 nsubjpass distribution,altered
R6413 T10195 T10194 auxpass was,altered
R6414 T10196 T10194 neg not,altered
R6415 T10197 T10194 prep from,altered
R6416 T10198 T10199 det the,pattern
R6417 T10199 T10197 pobj pattern,from
R6418 T10200 T10201 amod wild,type
R6419 T10201 T10199 compound type,pattern
R6420 T10202 T10201 punct -,type
R6421 T10203 T10194 punct .,altered
R6422 T10205 T10206 amod Elongated,mitochondria
R6423 T10206 T10211 nsubjpass mitochondria,observed
R6424 T10207 T10206 amod homozygous,mitochondria
R6425 T10208 T10206 nmod CbCln3Δex7,mitochondria
R6426 T10209 T10208 punct /,CbCln3Δex7
R6427 T10210 T10208 nummod 8,CbCln3Δex7
R6428 T10212 T10211 auxpass were,observed
R6429 T10213 T10211 advmod also,observed
R6430 T10214 T10211 prep by,observed
R6431 T10215 T10216 compound TEM,analysis
R6432 T10216 T10214 pobj analysis,by
R6433 T10217 T10211 punct .,observed
R6434 T10219 T10220 nummod 60,magnification
R6435 T10221 T10219 punct ×,60
R6436 T10222 T10220 punct .,magnification
R6437 T10224 T10225 meta b,shown
R6438 T10226 T10224 punct .,b
R6439 T10227 T10228 amod Cellular,ATP
R6440 T10228 T10229 compound ATP,levels
R6441 T10229 T10225 nsubjpass levels,shown
R6442 T10230 T10229 prep in,levels
R6443 T10231 T10232 amod wild,type
R6444 T10232 T10234 nmod type,cells
R6445 T10233 T10232 punct -,type
R6446 T10234 T10230 pobj cells,in
R6447 T10235 T10232 punct ", ",type
R6448 T10236 T10232 conj heterozygous,type
R6449 T10237 T10236 cc and,heterozygous
R6450 T10238 T10236 conj homozygous,heterozygous
R6451 T10239 T10234 nmod CbCln3Δex7,cells
R6452 T10240 T10239 punct /,CbCln3Δex7
R6453 T10241 T10239 nummod 8,CbCln3Δex7
R6454 T10242 T10234 compound precursor,cells
R6455 T10243 T10225 auxpass are,shown
R6456 T10244 T10225 punct .,shown
R6457 T10246 T10247 amod Wild,type
R6458 T10247 T10249 nmod type,cells
R6459 T10248 T10247 punct -,type
R6460 T10249 T10263 nsubj cells,contained
R6461 T10250 T10251 punct (,bar
R6462 T10251 T10247 parataxis bar,type
R6463 T10252 T10251 amod open,bar
R6464 T10253 T10251 punct ),bar
R6465 T10254 T10247 cc and,type
R6466 T10255 T10247 conj heterozygous,type
R6467 T10256 T10257 punct (,bar
R6468 T10257 T10255 parataxis bar,heterozygous
R6469 T10258 T10257 amod gray,bar
R6470 T10259 T10257 punct ),bar
R6471 T10260 T10249 nmod CbCln3Δex7,cells
R6472 T10261 T10260 punct /,CbCln3Δex7
R6473 T10262 T10260 nummod 8,CbCln3Δex7
R6474 T10264 T10265 punct ~,39
R6475 T10265 T10266 nummod 39,μM
R6476 T10266 T10267 compound μM,ATP
R6477 T10267 T10263 dobj ATP,contained
R6478 T10268 T10263 punct ", ",contained
R6479 T10269 T10270 mark while,contained
R6480 T10270 T10263 advcl contained,contained
R6481 T10271 T10272 amod homozygous,cells
R6482 T10272 T10270 nsubj cells,contained
R6483 T10273 T10272 nmod CbCln3Δex7,cells
R6484 T10274 T10273 punct /,CbCln3Δex7
R6485 T10275 T10273 nummod 8,CbCln3Δex7
R6486 T10276 T10277 punct (,bar
R6487 T10277 T10272 parataxis bar,cells
R6488 T10278 T10277 amod black,bar
R6489 T10279 T10277 punct ),bar
R6490 T10280 T10281 punct ~,1.3
R6491 T10281 T10282 npadvmod 1.3,reduced
R6492 T10282 T10284 amod reduced,levels
R6493 T10283 T10281 advmod fold,1.3
R6494 T10284 T10270 dobj levels,contained
R6495 T10285 T10284 prep of,levels
R6496 T10286 T10285 pobj ATP,of
R6497 T10287 T10284 punct (,levels
R6498 T10288 T10289 punct ~,30
R6499 T10289 T10290 nummod 30,μM
R6500 T10290 T10284 appos μM,levels
R6501 T10291 T10284 punct ),levels
R6502 T10292 T10284 punct ", ",levels
R6503 T10293 T10294 dep which,was
R6504 T10294 T10284 relcl was,levels
R6505 T10295 T10296 advmod statistically,significant
R6506 T10296 T10294 acomp significant,was
R6507 T10297 T10294 prep in,was
R6508 T10298 T10299 det a,test
R6509 T10299 T10297 pobj test,in
R6510 T10300 T10299 compound t,test
R6511 T10301 T10299 punct -,test
R6512 T10302 T10303 punct (,0.0001
R6513 T10303 T10294 parataxis 0.0001,was
R6514 T10304 T10303 nsubj p,0.0001
R6515 T10305 T10303 punct <,0.0001
R6516 T10306 T10303 punct ),0.0001
R6517 T10307 T10263 punct .,contained
R6518 T10309 T10310 amod Wild,type
R6519 T10310 T10312 nmod type,levels
R6520 T10311 T10310 punct -,type
R6532 T10324 T10323 prep from,different
R6533 T10325 T10326 det each,other
R6534 T10326 T10324 pobj other,from
R6535 T10327 T10328 punct (,0.4
R6536 T10328 T10320 parataxis 0.4,were
R6537 T10329 T10328 nsubj p,0.4
R6538 T10330 T10328 punct >,0.4
R6539 T10331 T10328 punct ),0.4
R6540 T10332 T10320 punct .,were
R6541 T10334 T10335 det A,representative
R6542 T10335 T10336 nsubjpass representative,shown
R6543 T10337 T10335 prep of,representative
R6544 T10338 T10339 amod triplicate,experiments
R6545 T10339 T10337 pobj experiments,of
R6546 T10340 T10336 auxpass is,shown
R6547 T10341 T10342 punct (,6
R6548 T10342 T10336 parataxis 6,shown
R6583 T10381 T10382 compound peroxide,treatment
R6584 T10382 T10379 pobj treatment,by
R6585 T10383 T10371 punct .,were
R6586 T10385 T10386 amod Wild,type
R6587 T10386 T10388 nmod type,cells
R6588 T10387 T10386 punct -,type
R6589 T10388 T10400 nsubj cells,exhibited
R6590 T10389 T10390 punct (,circle
R6591 T10390 T10386 parataxis circle,type
R6592 T10391 T10390 punct ),circle
R6593 T10392 T10386 cc and,type
R6594 T10393 T10386 conj heterozygous,type
R6595 T10394 T10395 punct (,triangle
R6596 T10395 T10393 parataxis triangle,heterozygous
R6597 T10396 T10395 punct ),triangle
R6598 T10397 T10388 nmod CbCln3Δex7,cells
R6599 T10398 T10397 punct /,CbCln3Δex7
R6600 T10399 T10397 nummod 8,CbCln3Δex7
R6601 T10401 T10402 punct ~,50
R6602 T10402 T10403 nummod 50,%
R6603 T10403 T10404 compound %,rates
R6617 T10417 T10416 nmod CbCln3Δex7,cells
R6618 T10418 T10417 punct /,CbCln3Δex7
R6619 T10419 T10417 nummod 8,CbCln3Δex7
R6620 T10420 T10421 punct (,squares
R6621 T10421 T10416 parataxis squares,cells
R6622 T10422 T10421 punct ),squares
R6633 T10433 T10400 punct .,exhibited
R6634 T10435 T10436 det A,representative
R6635 T10436 T10437 nsubjpass representative,shown
R6636 T10438 T10436 prep of,representative
R6637 T10439 T10440 amod triplicate,experiments
R6638 T10440 T10438 pobj experiments,of
R6639 T10441 T10437 auxpass is,shown
R6640 T10442 T10443 punct (,4
R6641 T10443 T10437 parataxis 4,shown
R6642 T10444 T10443 nsubj n,4
R6643 T10445 T10443 punct =,4
R6644 T10446 T10443 prep in,4
R6645 T10447 T10448 det each,experiment
R6646 T10448 T10446 pobj experiment,in
R6647 T10449 T10443 punct ),4
R6648 T10450 T10437 punct .,shown
R84 T263 T261 dobj battenin,express
R3 T170 T171 compound Membrane,trafficking
R4 T171 T173 nsubj trafficking,precede
R5 T174 T171 cc and,trafficking
R6 T175 T176 amod mitochondrial,abnormalities
R7 T176 T171 conj abnormalities,trafficking
R8 T177 T178 compound subunit,c
R9 T178 T179 compound c,deposition
R10 T179 T173 dobj deposition,precede
R11 T180 T173 prep in,precede
R12 T181 T182 det a,model
R14 T183 T184 amod cerebellar,cell
R15 T184 T182 compound cell,model
R16 T185 T182 prep of,model
R17 T186 T187 amod juvenile,lipofuscinosis
R18 T188 T187 amod neuronal,lipofuscinosis
R19 T189 T187 compound ceroid,lipofuscinosis
R20 T194 T195 nsubj JNCL,is
R21 T196 T197 det a,disease
R22 T197 T195 attr disease,is
R23 T198 T199 advmod recessively,inherited
R24 T199 T197 amod inherited,disease
R25 T200 T197 punct ", ",disease
R26 T201 T202 nmod childhood,onset
R27 T202 T197 nmod onset,disease
R28 T203 T202 punct -,onset
R29 T204 T197 amod neurodegenerative,disease
R30 T205 T206 advmod most,commonly
R32 T207 T206 punct -,commonly
R33 T208 T197 acl caused,disease
R34 T209 T208 agent by,caused
R35 T210 T211 det a,mutation
R36 T211 T209 pobj mutation,by
R37 T212 T213 punct ~,1
R38 T213 T214 nummod 1,kb
R39 T214 T211 compound kb,mutation
R40 T215 T211 compound CLN3,mutation
R41 T216 T195 punct .,is
R42 T218 T219 det The,loss
R44 T220 T219 amod resulting,loss
R45 T222 T219 prep of,loss
R46 T223 T224 compound battenin,activity
R47 T224 T222 pobj activity,of
R48 T225 T221 prep to,leads
R49 T226 T225 pobj deposition,to
R50 T227 T226 prep of,deposition
R51 T228 T229 amod mitochondrial,synthase
R53 T230 T229 compound ATP,synthase
R54 T231 T229 punct ", ",synthase
R55 T232 T233 compound subunit,c
R56 T233 T229 npadvmod c,synthase
R57 T234 T226 cc and,deposition
R58 T235 T236 det a,loss
R60 T237 T236 amod specific,loss
R61 T238 T236 prep of,loss
R62 T239 T240 compound CNS,neurons
R63 T240 T238 pobj neurons,of
R64 T241 T221 punct .,leads
R65 T243 T244 nsubj We,generated
R66 T245 T244 advmod previously,generated
R67 T246 T247 nmod Cln3Δex7,mice
R68 T247 T244 dobj mice,generated
R69 T248 T246 punct /,Cln3Δex7
R70 T249 T246 nummod 8,Cln3Δex7
R71 T250 T247 amod knock,mice
R72 T251 T250 punct -,knock
R73 T252 T250 prt in,knock
R74 T253 T247 punct ", ",mice
R75 T254 T255 dep which,replicate
R76 T255 T247 relcl replicate,mice
R77 T256 T257 det the,mutation
R78 T257 T255 dobj mutation,replicate
R79 T258 T257 amod common,mutation
R80 T259 T257 compound JNCL,mutation
R81 T260 T255 punct ", ",replicate
R82 T261 T255 conj express,replicate
R83 T262 T263 compound mutant,battenin
R87 T266 T267 npadvmod JNCL,like
R89 T268 T267 punct -,like
R90 T269 T265 dobj pathology,display
R91 T270 T244 punct .,generated
R92 T274 T275 aux To,elucidate
R93 T275 T276 advcl elucidate,used
R94 T277 T278 det the,consequences
R95 T278 T275 dobj consequences,elucidate
R96 T279 T278 prep of,consequences
R97 T280 T281 det the,mutation
R98 T281 T279 pobj mutation,of
R99 T282 T281 amod common,mutation
R100 T283 T281 compound JNCL,mutation
R101 T284 T278 prep in,consequences
R102 T285 T286 amod neuronal,cells
R103 T286 T284 pobj cells,in
R104 T287 T276 punct ", ",used
R105 T288 T276 nsubj we,used
R106 T289 T290 nmod P4,cerebella
R107 T290 T276 dobj cerebella,used
R108 T291 T290 amod knock,cerebella
R109 T292 T291 punct -,knock
R110 T293 T291 prt in,knock
R111 T294 T290 compound mouse,cerebella
R112 T295 T296 aux to,establish
R113 T296 T276 advcl establish,used
R114 T297 T298 advmod conditionally,immortalized
R115 T298 T299 amod immortalized,lines
R116 T299 T296 dobj lines,establish
R117 T300 T299 nmod CbCln3,lines
R118 T301 T302 amod wild,type
R119 T302 T299 nmod type,lines
R120 T303 T302 punct -,type
R121 T304 T302 punct ", ",type
R122 T305 T302 conj heterozygous,type
R123 T306 T305 punct ", ",heterozygous
R124 T307 T305 cc and,heterozygous
R125 T308 T305 conj homozygous,heterozygous
R126 T309 T310 amod neuronal,precursor
R127 T310 T299 compound precursor,lines
R128 T311 T299 compound cell,lines
R129 T312 T299 punct ", ",lines
R130 T313 T314 dep which,differentiated
R131 T314 T299 relcl differentiated,lines
R132 T315 T314 aux can,differentiated
R133 T316 T314 auxpass be,differentiated
R134 T317 T314 prep into,differentiated
R135 T318 T319 npadvmod MAP,positive
R136 T319 T325 amod positive,cells
R137 T320 T318 punct -,MAP
R138 T321 T318 nummod 2,MAP
R139 T322 T318 cc and,MAP
R140 T323 T318 conj NeuN,MAP
R141 T324 T319 punct -,positive
R142 T325 T317 pobj cells,into
R143 T326 T325 punct ", ",cells
R144 T327 T328 npadvmod neuron,like
R145 T328 T325 amod like,cells
R146 T329 T328 punct -,like
R147 T330 T276 punct .,used
R148 T332 T333 amod Homozygous,cells
R149 T333 T338 nsubj cells,express
R150 T334 T333 nmod CbCln3Δex7,cells
R151 T335 T334 punct /,CbCln3Δex7
R152 T336 T334 nummod 8,CbCln3Δex7
R153 T337 T333 compound precursor,cells
R154 T339 T340 amod low,levels
R155 T340 T338 dobj levels,express
R156 T341 T340 prep of,levels
R157 T342 T343 compound mutant,battenin
R158 T343 T341 pobj battenin,of
R159 T344 T338 cc and,express
R160 T345 T338 punct ", ",express
R161 T346 T347 advmod when,aged
R162 T347 T348 advcl aged,accumulate
R163 T348 T338 conj accumulate,express
R164 T349 T347 prep at,aged
R165 T350 T349 pobj confluency,at
R166 T351 T348 punct ", ",accumulate
R167 T352 T353 compound ATPase,c
R169 T354 T353 compound subunit,c
R170 T355 T338 punct .,express
R171 T357 T358 amod Recessive,phenotypes
R172 T358 T359 nsubjpass phenotypes,observed
R173 T360 T359 auxpass are,observed
R174 T361 T359 advmod also,observed
R175 T362 T359 prep at,observed
R176 T363 T364 amod sub-confluent,growth
R177 T364 T362 pobj growth,at
R178 T365 T359 punct ;,observed
R179 T366 T367 compound cathepsin,D
R180 T367 T368 nsubjpass D,altered
R181 T368 T359 conj altered,observed
R182 T369 T367 appos transport,D
R183 T370 T369 cc and,transport
R184 T371 T369 conj processing,transport
R185 T372 T368 auxpass are,altered
R186 T373 T368 punct ", ",altered
R187 T374 T375 mark although,affected
R188 T375 T381 advcl affected,altered
R189 T376 T377 compound enzyme,activity
R190 T377 T375 nsubjpass activity,affected
R191 T378 T375 auxpass is,affected
R192 T379 T375 neg not,affected
R193 T380 T375 advmod significantly,affected
R194 T381 T368 conj altered,altered
R195 T382 T381 punct ", ",altered
R196 T383 T384 amod lysosomal,size
R197 T384 T381 nsubjpass size,altered
R198 T385 T384 cc and,size
R199 T386 T384 conj distribution,size
R200 T387 T381 auxpass are,altered
R201 T388 T381 punct ", ",altered
R202 T389 T381 cc and,altered
R203 T390 T391 nsubjpass endocytosis,reduced
R205 T392 T391 auxpass is,reduced
R206 T393 T391 punct .,reduced
R207 T395 T396 prep In,are
R208 T397 T395 pobj addition,In
R209 T398 T396 punct ", ",are
R210 T399 T396 nsubj mitochondria,are
R211 T400 T401 advmod abnormally,elongated
R212 T401 T396 acomp elongated,are
R213 T402 T396 punct ", ",are
R214 T403 T404 amod cellular,levels
R216 T405 T404 compound ATP,levels
R218 T407 T406 auxpass are,decreased
R219 T408 T406 punct ", ",decreased
R220 T409 T406 cc and,decreased
R221 T410 T411 nsubjpass survival,reduced
R222 T411 T406 conj reduced,decreased
R223 T412 T410 prep following,survival
R224 T413 T414 amod oxidative,stress
R225 T414 T412 pobj stress,following
R226 T415 T411 auxpass is,reduced
R227 T416 T396 punct .,are
R228 T420 T421 det These,findings
R229 T421 T422 nsubj findings,reveal
R230 T423 T424 mark that,is
R232 T425 T424 nsubj battenin,is
R233 T426 T424 acomp required,is
R234 T427 T426 prep for,required
R235 T428 T429 amod intracellular,trafficking
R237 T430 T429 compound membrane,trafficking
R238 T431 T429 cc and,trafficking
R239 T432 T433 amod mitochondrial,function
R240 T433 T429 conj function,trafficking
R241 T434 T422 punct .,reveal
R242 T436 T437 advmod Moreover,are
R243 T438 T437 punct ", ",are
R244 T439 T440 det these,deficiencies
R245 T440 T437 nsubj deficiencies,are
R246 T441 T437 acomp likely,are
R247 T442 T443 aux to,be
R248 T443 T441 xcomp be,likely
R249 T444 T445 amod early,events
R250 T445 T443 attr events,be
R251 T446 T445 prep in,events
R252 T447 T448 det the,process
R253 T448 T446 pobj process,in
R254 T449 T448 compound JNCL,process
R255 T450 T448 compound disease,process
R256 T451 T437 cc and,are
R257 T452 T453 aux may,impact
R259 T454 T453 advmod particularly,impact
R260 T455 T456 amod neuronal,survival
R261 T456 T453 dobj survival,impact
R262 T457 T437 punct .,are
R263 T187 T185 pobj lipofuscinosis,of
R266 T696 T697 amod Juvenile,lipofuscinosis
R267 T697 T700 nsubj lipofuscinosis,is
R268 T698 T697 amod neuronal,lipofuscinosis
R269 T699 T697 compound ceroid,lipofuscinosis
R270 T701 T697 punct (,lipofuscinosis
R271 T702 T697 appos JNCL,lipofuscinosis
R272 T703 T697 punct ),lipofuscinosis
R273 T704 T697 punct ", ",lipofuscinosis
R274 T705 T697 cc or,lipofuscinosis
R275 T706 T707 compound Batten,disease
R276 T707 T697 conj disease,lipofuscinosis
R277 T708 T700 punct ", ",is
R278 T709 T710 det a,disorder
R279 T710 T700 attr disorder,is
R280 T711 T712 advmod recessively,inherited
R281 T712 T710 amod inherited,disorder
R282 T713 T710 nmod childhood,disorder
R283 T714 T713 punct -,childhood
R284 T715 T713 amod onset,childhood
R285 T716 T710 amod neurodegenerative,disorder
R286 T717 T710 acl characterized,disorder
R287 T718 T717 agent by,characterized
R288 T719 T720 amod progressive,blindness
R289 T720 T718 pobj blindness,by
R290 T721 T720 punct ", ",blindness
R291 T722 T720 conj seizures,blindness
R292 T723 T722 punct ", ",seizures
R293 T724 T725 nmod motor,decline
R294 T725 T722 conj decline,seizures
R295 T726 T724 cc and,motor
R296 T727 T724 conj cognitive,motor
R297 T728 T725 punct ", ",decline
R298 T729 T725 cc and,decline
R299 T730 T731 amod early,death
R300 T731 T725 conj death,decline
R301 T732 T733 punct [,1
R302 T733 T717 parataxis 1,characterized
R303 T734 T733 punct ],1
R304 T735 T700 punct .,is
R305 T737 T738 det The,defect
R306 T738 T741 nsubj defect,is
R307 T739 T738 amod primary,defect
R308 T740 T738 amod genetic,defect
R309 T742 T743 punct (,chromosomes
R311 T744 T745 punct >,80
R312 T745 T746 nummod 80,%
R313 T746 T743 compound %,chromosomes
R314 T747 T743 compound disease,chromosomes
R315 T748 T743 punct ),chromosomes
R316 T749 T738 acl leading,defect
R317 T750 T749 prep to,leading
R318 T751 T750 pobj JNCL,to
R319 T752 T753 det a,deletion
R321 T754 T755 nummod 1.02,kb
R322 T755 T753 nmod kb,deletion
R323 T756 T753 amod genomic,deletion
R324 T757 T753 compound DNA,deletion
R325 T758 T753 prep in,deletion
R326 T759 T760 det the,gene
R327 T760 T758 pobj gene,in
R328 T761 T760 compound CLN3,gene
R329 T762 T753 punct ", ",deletion
R330 T763 T764 dep which,eliminates
R331 T764 T753 relcl eliminates,deletion
R332 T765 T766 nmod exons,7
R333 T766 T764 dobj 7,eliminates
R334 T767 T766 cc and,7
R335 T768 T766 conj 8,7
R336 T769 T766 cc and,7
R337 T770 T771 amod surrounding,DNA
R338 T771 T766 conj DNA,7
R339 T772 T771 amod intronic,DNA
R340 T773 T741 punct ", ",is
R341 T774 T741 advcl predicting,is
R342 T775 T776 det a,product
R343 T776 T774 dobj product,predicting
R344 T777 T776 amod non-functional,product
R345 T778 T776 compound protein,product
R346 T779 T780 punct [,2
R347 T780 T741 parataxis 2,is
R348 T781 T780 punct ],2
R349 T782 T741 punct .,is
R350 T784 T785 det The,hallmark
R351 T785 T787 nsubj hallmark,is
R352 T786 T785 amod pathological,hallmark
R353 T788 T785 prep of,hallmark
R354 T789 T788 pobj JNCL,of
R355 T790 T791 amod autofluorescent,deposits
R356 T791 T787 attr deposits,is
R357 T792 T791 compound ceroid,deposits
R358 T793 T791 compound lipofuscin,deposits
R359 T794 T791 prep within,deposits
R360 T795 T794 pobj autolysosomes,within
R361 T796 T797 dep that,are
R362 T797 T791 relcl are,deposits
R363 T798 T797 acomp enriched,are
R364 T799 T797 prep in,are
R365 T800 T801 compound subunit,c
R366 T801 T799 pobj c,in
R367 T802 T801 prep of,c
R368 T803 T804 det the,complex
R369 T804 T802 pobj complex,of
R370 T805 T806 amod mitochondrial,synthase
R371 T806 T804 compound synthase,complex
R372 T807 T806 compound ATP,synthase
R373 T808 T809 punct [,3
R374 T809 T787 parataxis 3,is
R375 T810 T811 punct -,5
R376 T811 T809 prep 5,3
R377 T812 T809 punct ],3
R378 T813 T787 punct .,is
R379 T815 T816 advmod Remarkably,found
R380 T817 T816 punct ", ",found
R381 T818 T819 det these,deposits
R382 T819 T816 nsubjpass deposits,found
R383 T820 T816 auxpass are,found
R384 T821 T816 preconj not,found
R385 T822 T821 advmod only,not
R386 T823 T816 prep in,found
R387 T824 T825 compound CNS,neurons
R388 T825 T823 pobj neurons,in
R389 T826 T816 cc but,found
R390 T827 T816 conj are,found
R391 T828 T827 advmod also,are
R392 T829 T827 acomp abundant,are
R393 T830 T827 prep in,are
R394 T831 T832 amod non-neuronal,cells
R395 T832 T830 pobj cells,in
R396 T833 T832 prep outside,cells
R397 T834 T833 prep of,outside
R398 T835 T836 det the,system
R399 T836 T834 pobj system,of
R400 T837 T836 amod nervous,system
R401 T838 T816 punct .,found
R402 T840 T841 det The,relationship
R403 T841 T842 nsubj relationship,remain
R404 T843 T841 prep of,relationship
R405 T844 T845 compound subunit,c
R406 T845 T846 compound c,deposits
R407 T846 T843 pobj deposits,of
R408 T847 T841 prep to,relationship
R409 T848 T849 det the,process
R410 T849 T847 pobj process,to
R411 T850 T851 compound JNCL,disease
R412 T851 T849 compound disease,process
R413 T852 T841 punct ", ",relationship
R414 T853 T841 cc and,relationship
R415 T854 T855 det the,reason
R416 T855 T841 conj reason,relationship
R417 T856 T855 amod underlying,reason
R418 T857 T855 prep for,reason
R419 T858 T859 det the,specificity
R420 T859 T857 pobj specificity,for
R421 T860 T859 amod neuronal,specificity
R422 T861 T859 prep of,specificity
R423 T862 T863 det the,disease
R424 T863 T861 pobj disease,of
R425 T864 T865 advmod poorly,understood
R426 T865 T842 acomp understood,remain
R427 T866 T842 punct .,remain
R428 T868 T869 det The,protein
R429 T869 T873 nsubj protein,is
R430 T870 T871 npadvmod CLN3,encoded
R431 T871 T869 amod encoded,protein
R432 T872 T871 punct -,encoded
R433 T874 T869 punct (,protein
R434 T875 T869 appos battenin,protein
R435 T876 T875 punct ", ",battenin
R436 T877 T878 advmod also,called
R437 T878 T875 acl called,battenin
R438 T879 T878 oprd CLN3,called
R439 T880 T879 cc or,CLN3
R440 T881 T882 compound cln3,p
R441 T882 T879 conj p,CLN3
R442 T883 T873 punct ),is
R443 T884 T885 det a,protein
R444 T885 T873 attr protein,is
R445 T886 T887 advmod highly,conserved
R446 T887 T885 amod conserved,protein
R447 T888 T885 punct ", ",protein
R448 T889 T890 advmod ubiquitously,expressed
R449 T890 T885 amod expressed,protein
R450 T891 T885 punct ", ",protein
R451 T892 T885 compound multi-pass,protein
R452 T893 T885 compound membrane,protein
R453 T894 T895 punct [,6
R454 T895 T885 parataxis 6,protein
R455 T896 T895 punct ],6
R456 T897 T898 dep that,localizes
R457 T898 T885 relcl localizes,protein
R458 T899 T898 prep to,localizes
R459 T900 T901 det the,lysosome
R460 T901 T899 pobj lysosome,to
R461 T902 T901 cc and,lysosome
R462 T903 T904 amod other,compartments
R463 T904 T901 conj compartments,lysosome
R464 T905 T904 amod vesicular,compartments
R465 T906 T907 punct [,7
R466 T907 T898 parataxis 7,localizes
R467 T908 T909 punct -,9
R468 T909 T907 prep 9,7
R469 T910 T907 punct ],7
R470 T911 T873 punct .,is
R471 T913 T914 compound Battenin,function
R472 T914 T915 nsubj function,remains
R473 T916 T917 aux to,elucidated
R474 T917 T915 xcomp elucidated,remains
R475 T918 T917 auxpass be,elucidated
R476 T919 T917 punct ", ",elucidated
R477 T920 T921 mark although,implicated
R478 T921 T917 advcl implicated,elucidated
R479 T922 T921 nsubj studies,implicated
R480 T923 T922 prep of,studies
R481 T924 T923 pobj btn1,of
R482 T925 T924 punct ", ",btn1
R483 T926 T927 det the,ortholog
R484 T927 T924 appos ortholog,btn1
R485 T928 T927 compound yeast,ortholog
R486 T929 T927 compound CLN3,ortholog
R487 T930 T921 punct ", ",implicated
R488 T931 T921 aux have,implicated
R489 T932 T921 dobj battenin,implicated
R490 T933 T921 prep in,implicated
R491 T934 T935 amod lysosomal,homeostasis
R492 T935 T933 pobj homeostasis,in
R493 T936 T935 compound pH,homeostasis
R494 T937 T935 cc and,homeostasis
R495 T938 T939 compound amino,acid
R496 T939 T940 compound acid,transport
R497 T940 T935 conj transport,homeostasis
R498 T941 T942 punct [,11
R499 T942 T921 parataxis 11,implicated
R500 T943 T942 nummod 10,11
R501 T944 T942 punct ",",11
R502 T945 T942 punct ],11
R503 T946 T915 punct .,remains
R504 T948 T949 aux To,explore
R505 T949 T950 advcl explore,generated
R506 T951 T952 compound JNCL,pathogenesis
R507 T952 T949 dobj pathogenesis,explore
R508 T953 T952 cc and,pathogenesis
R509 T954 T955 compound battenin,function
R510 T955 T952 conj function,pathogenesis
R511 T956 T950 punct ", ",generated
R512 T957 T950 nsubj we,generated
R513 T958 T950 advmod previously,generated
R514 T959 T960 det a,model
R516 T961 T962 advmod genetically,precise
R517 T962 T960 amod precise,model
R518 T963 T960 compound JNCL,model
R519 T964 T960 compound mouse,model
R520 T965 T950 punct .,generated
R521 T967 T968 nmod Cln3Δex7,mice
R522 T968 T974 nsubj mice,harbor
R523 T969 T967 punct /,Cln3Δex7
R524 T970 T967 nummod 8,Cln3Δex7
R525 T971 T968 amod knock,mice
R526 T972 T971 punct -,knock
R527 T973 T971 prt in,knock
R528 T975 T976 det the,mutation
R529 T976 T974 dobj mutation,harbor
R530 T977 T978 punct ~,1
R531 T978 T979 nummod 1,kb
R532 T979 T976 nmod kb,mutation
R533 T980 T976 amod common,mutation
R534 T981 T976 compound JNCL,mutation
R535 T982 T974 cc and,harbor
R536 T983 T974 conj express,harbor
R537 T984 T985 det a,isoform
R538 T985 T983 dobj isoform,express
R539 T986 T985 amod non-truncated,isoform
R540 T987 T988 compound mutant,battenin
R541 T988 T985 compound battenin,isoform
R542 T989 T990 dep that,is
R543 T990 T985 relcl is,isoform
R544 T991 T990 acomp detectable,is
R545 T992 T990 prep with,is
R546 T993 T992 pobj antibodies,with
R547 T994 T993 acl recognizing,antibodies
R548 T995 T996 npadvmod C,terminal
R549 T996 T998 amod terminal,epitopes
R550 T997 T996 punct -,terminal
R551 T998 T994 dobj epitopes,recognizing
R552 T999 T974 punct .,harbor
R553 T1001 T1002 amod Homozygous,mice
R554 T1002 T1009 nsubj mice,exhibit
R555 T1003 T1002 nmod Cln3Δex7,mice
R556 T1004 T1003 punct /,Cln3Δex7
R557 T1005 T1003 nummod 8,Cln3Δex7
R558 T1006 T1002 amod knock,mice
R559 T1007 T1006 punct -,knock
R560 T1008 T1006 prt in,knock
R561 T1010 T1011 det a,disease
R563 T1012 T1011 amod progressive,disease
R564 T1013 T1014 npadvmod JNCL,like
R565 T1014 T1011 amod like,disease
R566 T1015 T1014 punct -,like
R567 T1016 T1011 punct ", ",disease
R568 T1017 T1011 prep with,disease
R569 T1018 T1019 amod perinatal,onset
R570 T1019 T1017 pobj onset,with
R571 T1020 T1019 prep of,onset
R572 T1021 T1022 compound subunit,c
R573 T1022 T1023 compound c,deposition
R574 T1023 T1020 pobj deposition,of
R575 T1024 T1023 prep in,deposition
R576 T1025 T1026 amod many,types
R577 T1026 T1024 pobj types,in
R578 T1027 T1026 compound cell,types
R579 T1028 T1019 cc and,onset
R580 T1029 T1030 amod later,onset
R581 T1030 T1019 conj onset,onset
R582 T1031 T1030 prep of,onset
R583 T1032 T1033 amod neuronal,dysfunction
R584 T1033 T1031 pobj dysfunction,of
R585 T1034 T1033 cc and,dysfunction
R586 T1035 T1036 amod behavioral,deficits
R587 T1036 T1033 conj deficits,dysfunction
R588 T1037 T1038 punct [,12
R589 T1038 T1009 parataxis 12,exhibit
R590 T1039 T1038 punct ],12
R591 T1040 T1009 punct .,exhibit
R592 T1042 T1043 det These,findings
R593 T1043 T1044 nsubj findings,suggest
R594 T1045 T1046 mark that,leads
R596 T1047 T1048 det the,defect
R597 T1048 T1046 nsubj defect,leads
R598 T1049 T1048 amod major,defect
R599 T1050 T1048 compound JNCL,defect
R600 T1051 T1046 prep to,leads
R601 T1052 T1053 amod abnormal,turnover
R602 T1053 T1051 pobj turnover,to
R603 T1054 T1053 prep of,turnover
R604 T1055 T1056 amod mitochondrial,c
R605 T1056 T1054 pobj c,of
R606 T1057 T1056 compound subunit,c
R607 T1058 T1046 punct ", ",leads
R608 T1059 T1046 prep in,leads
R609 T1060 T1061 det a,manner
R610 T1061 T1059 pobj manner,in
R611 T1062 T1063 dep that,compromises
R612 T1063 T1061 relcl compromises,manner
R613 T1064 T1063 advmod selectively,compromises
R614 T1065 T1066 compound CNS,neurons
R615 T1066 T1063 dobj neurons,compromises
R616 T1067 T1044 punct .,suggest
R617 T1069 T1070 advmod Currently,is
R618 T1071 T1070 punct ", ",is
R619 T1072 T1070 expl there,is
R620 T1073 T1074 det no,system
R621 T1074 T1070 attr system,is
R622 T1075 T1074 amod suitable,system
R623 T1076 T1077 amod neuronal,cell
R624 T1077 T1074 compound cell,system
R625 T1078 T1079 aux to,investigate
R626 T1079 T1074 advcl investigate,system
R627 T1080 T1081 det the,impact
R628 T1081 T1079 dobj impact,investigate
R629 T1082 T1081 prep of,impact
R630 T1083 T1084 det the,mutation
R631 T1084 T1082 pobj mutation,of
R632 T1085 T1084 amod common,mutation
R633 T1086 T1084 compound JNCL,mutation
R634 T1087 T1081 prep on,impact
R635 T1088 T1089 amod biological,processes
R636 T1089 T1087 pobj processes,on
R637 T1090 T1070 punct .,is
R638 T1092 T1093 advmod Therefore,established
R639 T1094 T1093 punct ", ",established
R640 T1095 T1093 nsubj we,established
R641 T1096 T1093 aux have,established
R642 T1097 T1098 amod cerebellar,lines
R643 T1098 T1093 dobj lines,established
R644 T1099 T1098 amod neuronal,lines
R645 T1100 T1098 compound precursor,lines
R646 T1101 T1098 compound cell,lines
R647 T1102 T1093 prep from,established
R648 T1103 T1104 nmod Cln3Δex7,mice
R649 T1104 T1102 pobj mice,from
R650 T1105 T1103 punct /,Cln3Δex7
R651 T1106 T1103 nummod 8,Cln3Δex7
R652 T1107 T1104 amod knock,mice
R653 T1108 T1107 punct -,knock
R654 T1109 T1107 prt in,knock
R655 T1110 T1093 punct .,established
R656 T1112 T1113 amod Homozygous,cells
R657 T1113 T1117 nsubj cells,exhibit
R658 T1114 T1113 nmod CbCln3Δex7,cells
R659 T1115 T1114 punct /,CbCln3Δex7
R660 T1116 T1114 nummod 8,CbCln3Δex7
R661 T1118 T1119 amod pathological,hallmarks
R662 T1119 T1117 dobj hallmarks,exhibit
R663 T1120 T1119 prep of,hallmarks
R664 T1121 T1122 det the,disease
R665 T1122 T1120 pobj disease,of
R666 T1123 T1117 punct ", ",exhibit
R667 T1124 T1117 cc and,exhibit
R668 T1125 T1126 det a,survey
R669 T1126 T1127 nsubj survey,revealed
R670 T1127 T1117 conj revealed,exhibit
R671 T1128 T1126 prep of,survey
R672 T1129 T1130 compound membrane,organelles
R673 T1130 T1128 pobj organelles,of
R674 T1131 T1132 npadvmod membrane,trafficking
R675 T1132 T1133 amod trafficking,defects
R676 T1133 T1127 dobj defects,revealed
R677 T1134 T1133 cc and,defects
R678 T1135 T1136 amod mitochondrial,dysfunction
R679 T1136 T1133 conj dysfunction,defects
R680 T1137 T1127 prep in,revealed
R681 T1138 T1139 amod homozygous,mutant
R682 T1139 T1140 nmod mutant,cells
R683 T1140 T1137 pobj cells,in
R684 T1141 T1140 nmod CbCln3Δex7,cells
R685 T1142 T1141 punct /,CbCln3Δex7
R686 T1143 T1141 nummod 8,CbCln3Δex7
R687 T1144 T1127 punct .,revealed
R688 T1272 T1271 prep of,Generation
R689 T1273 T1274 det a,model
R690 T1274 T1272 pobj model,of
R691 T1275 T1276 advmod genetically,precise
R692 T1276 T1274 amod precise,model
R693 T1277 T1274 amod cerebellar,model
R694 T1278 T1274 compound JNCL,model
R695 T1279 T1274 compound cell,model
R696 T1281 T1282 aux To,generate
R697 T1282 T1283 advcl generate,immortalized
R698 T1284 T1285 det a,system
R699 T1285 T1282 dobj system,generate
R700 T1286 T1285 amod precise,system
R701 T1287 T1285 amod genetic,system
R702 T1288 T1285 punct ", ",system
R703 T1289 T1290 npadvmod neuron,derived
R704 T1290 T1285 amod derived,system
R705 T1291 T1290 punct -,derived
R706 T1292 T1293 compound JNCL,culture
R709 T1295 T1283 punct ", ",immortalized
R710 T1296 T1283 nsubj we,immortalized
R711 T1297 T1298 compound granule,neurons
R712 T1298 T1283 dobj neurons,immortalized
R714 T1300 T1299 prep from,cultured
R715 T1301 T1302 amod postnatal,day
R716 T1302 T1303 nmod day,cerebella
R718 T1304 T1302 nummod 4,day
R719 T1305 T1302 punct (,day
R720 T1306 T1302 appos P4,day
R721 T1307 T1303 punct ),cerebella
R722 T1308 T1303 prep of,cerebella
R723 T1309 T1310 amod homozygous,mice
R724 T1310 T1308 pobj mice,of
R725 T1311 T1309 cc and,homozygous
R726 T1312 T1309 conj heterozygous,homozygous
R727 T1313 T1310 nmod Cln3Δex7,mice
R728 T1314 T1313 punct /,Cln3Δex7
R729 T1315 T1313 nummod 8,Cln3Δex7
R730 T1316 T1310 amod knock,mice
R731 T1317 T1316 punct -,knock
R732 T1318 T1316 prt in,knock
R733 T1319 T1310 punct ", ",mice
R734 T1320 T1310 cc and,mice
R735 T1321 T1322 amod wild,type
R736 T1322 T1324 compound type,littermates
R737 T1323 T1322 punct -,type
R738 T1324 T1310 conj littermates,mice
R739 T1325 T1283 punct .,immortalized
R740 T1327 T1328 amod Primary,cultures
R741 T1328 T1330 nsubjpass cultures,transduced
R742 T1329 T1328 compound cell,cultures
R743 T1331 T1328 acl enriched,cultures
R744 T1332 T1331 prep for,enriched
R745 T1333 T1334 compound granule,neurons
R746 T1334 T1332 pobj neurons,for
R747 T1335 T1330 auxpass were,transduced
R748 T1336 T1330 prep with,transduced
R749 T1337 T1338 amod retroviral,vector
R750 T1338 T1336 pobj vector,with
R751 T1339 T1338 acl bearing,vector
R752 T1340 T1341 det a,cassette
R753 T1341 T1339 dobj cassette,bearing
R754 T1342 T1341 compound selection,cassette
R755 T1343 T1341 cc and,cassette
R756 T1344 T1345 npadvmod temperature,sensitive
R757 T1345 T1347 amod sensitive,antigen
R758 T1346 T1345 punct -,sensitive
R760 T1348 T1349 nmod tsA58,SV40
R761 T1349 T1347 nmod SV40,antigen
R762 T1350 T1347 amod large,antigen
R763 T1351 T1347 compound T,antigen
R764 T1352 T1330 punct .,transduced
R765 T1354 T1355 nsubj Growth,allowed
R766 T1356 T1354 prep in,Growth
R767 T1357 T1358 npadvmod G418,containing
R768 T1358 T1359 amod containing,medium
R769 T1359 T1356 pobj medium,in
R770 T1360 T1354 prep at,Growth
R771 T1361 T1362 det the,temperature
R772 T1362 T1360 pobj temperature,at
R773 T1363 T1362 amod permissive,temperature
R774 T1364 T1362 punct (,temperature
R775 T1365 T1366 nummod 33,°C
R776 T1366 T1362 appos °C,temperature
R777 T1367 T1355 punct ),allowed
R778 T1368 T1355 prep for,allowed
R779 T1369 T1368 pobj selection,for
R780 T1370 T1369 cc and,selection
R781 T1371 T1369 conj isolation,selection
R782 T1372 T1369 prep of,selection
R783 T1373 T1374 amod multiple,lines
R784 T1374 T1372 pobj lines,of
R785 T1375 T1376 amod clonal,nestin
R786 T1376 T1377 npadvmod nestin,positive
R787 T1377 T1374 amod positive,lines
R788 T1378 T1377 punct -,positive
R789 T1379 T1380 punct (,1a
R790 T1380 T1377 parataxis 1a,positive
R791 T1381 T1380 compound Fig.,1a
R792 T1382 T1380 punct ),1a
R793 T1383 T1377 punct ", ",positive
R794 T1384 T1377 cc and,positive
R795 T1385 T1386 npadvmod GFAP,negative
R796 T1386 T1377 conj negative,positive
R797 T1387 T1386 punct -,negative
R798 T1388 T1389 punct (,1b
R799 T1389 T1386 parataxis 1b,negative
R800 T1390 T1389 compound Fig.,1b
R801 T1391 T1389 punct ),1b
R802 T1392 T1374 punct ", ",lines
R803 T1393 T1374 compound cell,lines
R804 T1394 T1374 prep for,lines
R805 T1395 T1396 det each,genotype
R806 T1396 T1394 pobj genotype,for
R807 T1397 T1355 punct .,allowed
R808 T1399 T1400 det No,differences
R809 T1400 T1403 nsubjpass differences,observed
R810 T1401 T1400 nmod genotype,differences
R811 T1402 T1400 amod specific,differences
R812 T1404 T1403 auxpass were,observed
R813 T1405 T1403 prep in,observed
R814 T1406 T1407 amod cellular,morphology
R815 T1407 T1405 pobj morphology,in
R816 T1408 T1407 cc or,morphology
R817 T1409 T1410 compound doubling,time
R818 T1410 T1407 conj time,morphology
R819 T1411 T1410 punct (,time
R820 T1412 T1413 punct ~,46
R821 T1413 T1414 nummod 46,hours
R822 T1414 T1410 appos hours,time
R823 T1415 T1403 punct ),observed
R824 T1416 T1417 punct (,shown
R825 T1417 T1403 parataxis shown,observed
R826 T1418 T1417 nsubj data,shown
R827 T1419 T1417 neg not,shown
R828 T1420 T1417 punct ),shown
R829 T1421 T1403 punct .,observed
R830 T1423 T1424 mark As,expected
R831 T1424 T1425 advcl expected,lost
R832 T1426 T1425 punct ", ",lost
R833 T1427 T1428 nmod SV40,expression
R834 T1428 T1425 nsubjpass expression,lost
R835 T1429 T1428 amod large,expression
R836 T1430 T1431 compound T,antigen
R837 T1431 T1428 compound antigen,expression
R838 T1432 T1425 auxpass was,lost
R839 T1433 T1425 advmod rapidly,lost
R840 T1434 T1425 cc and,lost
R841 T1435 T1436 compound cell,division
R842 T1436 T1437 nsubj division,ceased
R843 T1437 T1425 conj ceased,lost
R844 T1438 T1439 advmod when,shifted
R845 T1439 T1437 advcl shifted,ceased
R846 T1440 T1439 nsubjpass cells,shifted
R847 T1441 T1439 auxpass were,shifted
R848 T1442 T1439 prep to,shifted
R849 T1443 T1444 det the,temperature
R850 T1444 T1442 pobj temperature,to
R851 T1445 T1444 amod non-permissive,temperature
R852 T1446 T1444 punct (,temperature
R853 T1447 T1448 nummod 39,°C
R854 T1448 T1444 appos °C,temperature
R855 T1449 T1439 punct ),shifted
R856 T1450 T1451 punct (,shown
R857 T1451 T1439 parataxis shown,shifted
R858 T1452 T1451 nsubj data,shown
R859 T1453 T1451 neg not,shown
R860 T1454 T1451 punct ),shown
R861 T1455 T1437 punct .,ceased
R862 T1457 T1458 prep Upon,became
R863 T1459 T1457 pobj addition,Upon
R864 T1460 T1459 prep of,addition
R865 T1461 T1462 amod neuronal,differentiation
R866 T1462 T1463 compound differentiation,cocktail
R867 T1463 T1460 pobj cocktail,of
R868 T1464 T1458 punct ", ",became
R869 T1465 T1466 compound precursor,cells
R870 T1466 T1458 nsubj cells,became
R871 T1467 T1468 npadvmod neuron,like
R872 T1468 T1458 acomp like,became
R873 T1469 T1468 punct -,like
R874 T1470 T1458 prep in,became
R875 T1471 T1470 pobj morphology,in
R876 T1472 T1458 cc and,became
R877 T1473 T1458 conj exhibited,became
R878 T1474 T1475 amod decreased,expression
R879 T1475 T1473 dobj expression,exhibited
R880 T1476 T1475 compound nestin,expression
R881 T1477 T1478 punct (,shown
R882 T1478 T1475 parataxis shown,expression
R883 T1479 T1478 nsubj data,shown
R884 T1480 T1478 neg not,shown
R885 T1481 T1478 punct ),shown
R886 T1482 T1475 cc and,expression
R887 T1483 T1484 amod increased,expression
R888 T1484 T1475 conj expression,expression
R889 T1485 T1484 nmod MAP2,expression
R890 T1486 T1485 cc and,MAP2
R891 T1487 T1485 conj NeuN,MAP2
R892 T1488 T1489 punct (,1d
R893 T1489 T1484 parataxis 1d,expression
R894 T1490 T1489 nmod Fig.,1d
R895 T1491 T1489 nmod 1c,1d
R896 T1492 T1489 punct ",",1d
R897 T1493 T1489 punct ),1d
R898 T1494 T1475 punct ", ",expression
R899 T1495 T1475 cc but,expression
R900 T1496 T1495 neg not,but
R901 T1497 T1475 conj expression,expression
R902 T1498 T1497 prep of,expression
R903 T1499 T1500 det the,marker
R904 T1500 T1498 pobj marker,of
R905 T1501 T1500 compound Purkinje,marker
R906 T1502 T1500 punct ", ",marker
R907 T1503 T1500 appos calbindin,marker
R908 T1504 T1505 punct (,1e
R909 T1505 T1497 parataxis 1e,expression
R910 T1506 T1505 compound Fig.,1e
R911 T1507 T1505 punct ),1e
R912 T1508 T1458 punct .,became
R913 T1775 T1776 amod Homozygous,cells
R914 T1776 T1780 nsubj cells,express
R915 T1777 T1776 nmod CbCln3Δex7,cells
R916 T1778 T1777 punct /,CbCln3Δex7
R917 T1779 T1777 nummod 8,CbCln3Δex7
R918 T1781 T1782 compound mutant,battenin
R919 T1782 T1780 dobj battenin,express
R920 T1783 T1780 cc and,express
R921 T1784 T1780 conj display,express
R922 T1785 T1786 npadvmod JNCL,like
R923 T1786 T1788 amod like,pathology
R924 T1787 T1786 punct -,like
R925 T1788 T1784 dobj pathology,display
R926 T1790 T1791 amod Homozygous,cells
R927 T1791 T1796 nsubjpass cells,examined
R928 T1792 T1793 nmod Cb,Cln3Δex7
R929 T1793 T1791 nmod Cln3Δex7,cells
R930 T1794 T1793 punct /,Cln3Δex7
R931 T1795 T1793 nummod 8,Cln3Δex7
R932 T1797 T1796 auxpass were,examined
R933 T1798 T1796 advmod first,examined
R934 T1799 T1796 prep for,examined
R935 T1800 T1801 npadvmod JNCL,like
R936 T1801 T1803 amod like,characteristics
R937 T1802 T1801 punct -,like
R938 T1803 T1799 pobj characteristics,for
R939 T1804 T1796 punct .,examined
R940 T1806 T1807 amod Homozygous,mice
R941 T1807 T1814 nsubj mice,express
R942 T1808 T1807 nmod Cln3Δex7,mice
R943 T1809 T1808 punct /,Cln3Δex7
R944 T1810 T1808 nummod 8,Cln3Δex7
R945 T1811 T1807 amod knock,mice
R946 T1812 T1811 punct -,knock
R947 T1813 T1811 prt in,knock
R948 T1815 T1816 amod multiple,variants
R949 T1816 T1814 dobj variants,express
R950 T1817 T1818 compound Cln3,splice
R951 T1818 T1816 compound splice,variants
R952 T1819 T1818 compound mRNA,splice
R953 T1820 T1816 cc and,variants
R954 T1821 T1822 compound mutant,protein
R955 T1822 T1816 conj protein,variants
R956 T1823 T1822 compound battenin,protein
R957 T1824 T1825 dep that,is
R958 T1825 T1822 relcl is,protein
R959 T1826 T1825 acomp detectable,is
R960 T1827 T1825 prep by,is
R961 T1828 T1829 compound batp1,antibody
R962 T1829 T1830 npadvmod antibody,recognizing
R963 T1830 T1831 amod recognizing,epitopes
R964 T1831 T1827 pobj epitopes,by
R965 T1832 T1833 npadvmod C,terminal
R966 T1833 T1831 amod terminal,epitopes
R967 T1834 T1833 punct -,terminal
R968 T1835 T1836 punct [,12
R969 T1836 T1814 parataxis 12,express
R970 T1837 T1836 punct ],12
R971 T1838 T1814 punct .,express
R972 T1840 T1841 aux To,assess
R973 T1841 T1842 advcl assess,performed
R974 T1843 T1844 det this,phenotype
R975 T1844 T1841 dobj phenotype,assess
R976 T1845 T1844 amod molecular,phenotype
R977 T1846 T1841 prep in,assess
R978 T1847 T1848 nmod CbCln3Δex7,cells
R979 T1848 T1846 pobj cells,in
R980 T1849 T1847 punct /,CbCln3Δex7
R981 T1850 T1847 nummod 8,CbCln3Δex7
R982 T1851 T1842 punct ", ",performed
R983 T1852 T1853 compound RT,PCR
R984 T1853 T1842 nsubjpass PCR,performed
R985 T1854 T1853 punct -,PCR
R986 T1855 T1853 cc and,PCR
R987 T1856 T1857 nmod anti-battenin,immunostaining
R988 T1857 T1853 conj immunostaining,PCR
R989 T1858 T1856 punct (,anti-battenin
R990 T1859 T1856 appos batp1,anti-battenin
R991 T1860 T1857 punct ),immunostaining
R992 T1861 T1842 auxpass were,performed
R993 T1862 T1842 punct .,performed
R994 T1864 T1865 mark As,shown
R995 T1865 T1866 advcl shown,were
R996 T1867 T1865 prep in,shown
R997 T1868 T1867 pobj Figure,in
R998 T1869 T1868 nummod 2,Figure
R999 T1870 T1866 punct ", ",were
R1000 T1871 T1872 compound Cln3,isoforms
R1001 T1872 T1866 nsubj isoforms,were
R1002 T1873 T1872 compound mRNA,isoforms
R1003 T1874 T1872 prep in,isoforms
R1004 T1875 T1876 amod wild,type
R1005 T1876 T1878 nmod type,cells
R1006 T1877 T1876 punct -,type
R1007 T1878 T1874 pobj cells,in
R1008 T1879 T1876 cc and,type
R1009 T1880 T1876 conj homozygous,type
R1010 T1881 T1866 acomp similar,were
R1011 T1882 T1881 prep to,similar
R1012 T1883 T1882 pobj those,to
R1013 T1884 T1883 acl observed,those
R1014 T1885 T1884 prep in,observed
R1015 T1886 T1887 amod total,RNA
R1016 T1887 T1885 pobj RNA,in
R1017 T1888 T1887 acl isolated,RNA
R1018 T1889 T1888 prep from,isolated
R1019 T1890 T1891 amod wild,type
R1020 T1891 T1893 nmod type,brain
R1021 T1892 T1891 punct -,type
R1022 T1893 T1889 pobj brain,from
R1023 T1894 T1891 cc or,type
R1024 T1895 T1891 conj homozygous,type
R1025 T1896 T1893 nmod Cln3Δex7,brain
R1026 T1897 T1896 punct /,Cln3Δex7
R1027 T1898 T1896 nummod 8,Cln3Δex7
R1028 T1899 T1893 amod knock,brain
R1029 T1900 T1899 punct -,knock
R1030 T1901 T1899 prt in,knock
R1031 T1902 T1866 punct ", ",were
R1032 T1903 T1866 advmod respectively,were
R1033 T1904 T1905 punct (,Fig.
R1034 T1905 T1866 parataxis Fig.,were
R1035 T1906 T1905 nummod 2,Fig.
R1036 T1907 T1905 punct ),Fig.
R1037 T1908 T1866 punct .,were
R1038 T1910 T1911 prep In,detected
R1039 T1912 T1910 pobj addition,In
R1040 T1913 T1911 punct ", ",detected
R1041 T1914 T1915 compound batp1,immunostaining
R1042 T1915 T1911 nsubj immunostaining,detected
R1043 T1916 T1917 compound mutant,product
R1044 T1917 T1911 dobj product,detected
R1045 T1918 T1917 compound battenin,product
R1046 T1919 T1917 prep in,product
R1047 T1920 T1921 amod homozygous,cells
R1048 T1921 T1919 pobj cells,in
R1049 T1922 T1921 nmod CbCln3Δex7,cells
R1050 T1923 T1922 punct /,CbCln3Δex7
R1051 T1924 T1922 nummod 8,CbCln3Δex7
R1052 T1925 T1917 punct ", ",product
R1053 T1926 T1917 prep in,product
R1054 T1927 T1928 det a,pattern
R1055 T1928 T1926 pobj pattern,in
R1056 T1929 T1928 amod similar,pattern
R1057 T1930 T1931 mark albeit,reduced
R1058 T1931 T1929 parataxis reduced,similar
R1059 T1932 T1928 amod cytoplasmic,pattern
R1060 T1933 T1928 punct ", ",pattern
R1061 T1934 T1928 amod vesicular,pattern
R1062 T1935 T1928 compound staining,pattern
R1063 T1936 T1928 prep as,pattern
R1064 T1937 T1936 pobj that,as
R1065 T1938 T1937 acl seen,that
R1066 T1939 T1938 prep in,seen
R1067 T1940 T1941 amod wild,type
R1068 T1941 T1943 compound type,cells
R1069 T1942 T1941 punct -,type
R1070 T1943 T1939 pobj cells,in
R1071 T1944 T1911 punct .,detected
R1072 T1946 T1947 compound Batp1,signal
R1073 T1947 T1948 nsubj signal,exhibited
R1074 T1949 T1950 det some,overlap
R1075 T1950 T1948 dobj overlap,exhibited
R1076 T1951 T1948 prep with,exhibited
R1077 T1952 T1953 det the,marker
R1078 T1953 T1951 pobj marker,with
R1079 T1954 T1953 amod lysosomal,marker
R1080 T1955 T1953 punct ", ",marker
R1081 T1956 T1953 appos Lamp1,marker
R1082 T1957 T1948 punct ", ",exhibited
R1083 T1958 T1948 cc but,exhibited
R1084 T1959 T1948 conj had,exhibited
R1085 T1960 T1961 advmod more,significant
R1086 T1961 T1962 amod significant,overlap
R1087 T1962 T1959 dobj overlap,had
R1088 T1963 T1959 prep with,had
R1089 T1964 T1965 amod early,antigen
R1090 T1965 T1963 pobj antigen,with
R1091 T1966 T1965 compound endosome,antigen
R1092 T1967 T1965 nummod 1,antigen
R1093 T1968 T1965 punct (,antigen
R1094 T1969 T1965 appos EEA1,antigen
R1095 T1970 T1965 punct ),antigen
R1096 T1971 T1965 cc and,antigen
R1097 T1972 T1973 det the,marker
R1098 T1973 T1965 conj marker,antigen
R1099 T1974 T1973 amod late,marker
R1100 T1975 T1973 amod endosomal,marker
R1101 T1976 T1973 punct ", ",marker
R1102 T1977 T1973 appos Rab7,marker
R1103 T1978 T1979 punct (,Fig.
R1104 T1979 T1959 parataxis Fig.,had
R1105 T1980 T1979 nummod 3,Fig.
R1106 T1981 T1979 punct ),Fig.
R1107 T1982 T1948 punct .,exhibited
R1108 T1984 T1985 advmod Only,overlap
R1109 T1985 T1987 nsubjpass overlap,observed
R1110 T1986 T1985 amod limited,overlap
R1111 T1988 T1987 auxpass was,observed
R1112 T1989 T1987 prep with,observed
R1113 T1990 T1991 amod recycling,endosomes
R1114 T1991 T1989 pobj endosomes,with
R1115 T1992 T1987 punct ", ",observed
R1116 T1993 T1994 mark as,determined
R1117 T1994 T1987 advcl determined,observed
R1118 T1995 T1994 agent by,determined
R1119 T1996 T1997 compound transferrin,receptor
R1120 T1997 T1998 compound receptor,co-staining
R1121 T1998 T1995 pobj co-staining,by
R1122 T1999 T2000 punct (,shown
R1123 T2000 T1987 parataxis shown,observed
R1124 T2001 T2000 nsubj data,shown
R1125 T2002 T2000 neg not,shown
R1126 T2003 T2000 punct ),shown
R1127 T2004 T1987 punct .,observed
R1128 T2006 T2007 advmod Intriguingly,altered
R1129 T2008 T2007 punct ", ",altered
R1130 T2009 T2010 nmod Lamp1,distribution
R1131 T2010 T2007 nsubjpass distribution,altered
R1132 T2011 T2009 cc and,Lamp1
R1133 T2012 T2009 conj EEA1,Lamp1
R1134 T2013 T2010 amod immunocytochemical,distribution
R1135 T2014 T2007 auxpass were,altered
R1136 T2015 T2007 prep in,altered
R1137 T2016 T2017 amod homozygous,cells
R1138 T2017 T2015 pobj cells,in
R1139 T2018 T2017 nmod CbCln3Δex7,cells
R1140 T2019 T2018 punct /,CbCln3Δex7
R1141 T2020 T2018 nummod 8,CbCln3Δex7
R1142 T2021 T2007 punct ", ",altered
R1143 T2022 T2007 prep with,altered
R1144 T2023 T2024 amod less,clustering
R1145 T2024 T2022 pobj clustering,with
R1146 T2025 T2024 amod perinuclear,clustering
R1147 T2026 T2024 prep than,clustering
R1148 T2027 T2026 prep in,than
R1149 T2028 T2029 amod wild,type
R1150 T2029 T2031 compound type,cells
R1151 T2030 T2029 punct -,type
R1152 T2031 T2027 pobj cells,in
R1153 T2032 T2007 punct ", ",altered
R1154 T2033 T2007 cc and,altered
R1155 T2034 T2035 compound Rab7,staining
R1156 T2035 T2036 nsubj staining,was
R1157 T2036 T2007 conj was,altered
R1158 T2037 T2036 advmod frequently,was
R1159 T2038 T2039 advmod less,intense
R1160 T2039 T2036 acomp intense,was
R1161 T2040 T2039 prep in,intense
R1162 T2041 T2042 amod homozygous,cells
R1163 T2042 T2040 pobj cells,in
R1164 T2043 T2042 nmod CbCln3Δex7,cells
R1165 T2044 T2043 punct /,CbCln3Δex7
R1166 T2045 T2043 nummod 8,CbCln3Δex7
R1167 T2046 T2047 punct (,Fig.
R1168 T2047 T2036 parataxis Fig.,was
R1169 T2048 T2047 nummod 3,Fig.
R1170 T2049 T2047 punct ),Fig.
R1171 T2050 T2036 punct .,was
R1211 T2092 T2090 neg not,shown
R1212 T2093 T2090 punct ),shown
R1213 T2094 T2079 punct .,was
R1240 T2123 T2097 punct .,displayed
R1241 T2125 T2126 advmod However,elevated
R1242 T2127 T2126 punct ", ",elevated
R1243 T2128 T2129 advmod when,aged
R1244 T2129 T2126 advcl aged,elevated
R1245 T2130 T2129 nsubjpass cells,aged
R1246 T2131 T2129 auxpass were,aged
R1247 T2132 T2129 prep at,aged
R1248 T2133 T2132 pobj confluency,at
R1249 T2134 T2133 punct (,confluency
R1250 T2135 T2136 nummod 3,days
R1251 T2136 T2138 npadvmod days,post-confluency
R1252 T2137 T2135 punct +,3
R1253 T2138 T2133 advmod post-confluency,confluency
R1254 T2139 T2126 punct ),elevated
R1255 T2140 T2126 punct ", ",elevated
R1256 T2141 T2142 amod homozygous,levels
R1257 T2142 T2126 nsubjpass levels,elevated
R1258 T2143 T2142 nmod CbCln3Δex7,levels
R1259 T2144 T2143 punct /,CbCln3Δex7
R1260 T2145 T2143 nummod 8,CbCln3Δex7
R1261 T2146 T2142 amod cellular,levels
R1262 T2147 T2148 compound subunit,c
R1263 T2148 T2142 compound c,levels
R1264 T2149 T2126 auxpass were,elevated
R1265 T2150 T2126 prep beyond,elevated
R1266 T2151 T2152 amod normal,levels
R1267 T2152 T2150 pobj levels,beyond
R1268 T2153 T2154 amod wild,type
R1269 T2154 T2152 compound type,levels
R1270 T2155 T2154 punct -,type
R1271 T2156 T2126 prep by,elevated
R1272 T2157 T2156 pobj immunostaining,by
R1273 T2158 T2159 punct (,4a
R1274 T2159 T2157 parataxis 4a,immunostaining
R1275 T2160 T2159 compound Fig.,4a
R1276 T2161 T2159 punct ),4a
R1277 T2162 T2157 cc and,immunostaining
R1278 T2163 T2164 compound immunoblot,analysis
R1279 T2164 T2157 conj analysis,immunostaining
R1280 T2165 T2166 punct (,4b
R1281 T2166 T2164 parataxis 4b,analysis
R1282 T2167 T2166 compound Fig.,4b
R1283 T2168 T2166 punct ),4b
R1284 T2169 T2126 punct .,elevated
R1285 T2171 T2172 amod Autofluorescent,signal
R1286 T2172 T2173 nsubj signal,overlapped
R1287 T2174 T2173 advmod sometimes,overlapped
R1288 T2175 T2173 prep with,overlapped
R1289 T2176 T2177 compound subunit,c
R1290 T2177 T2178 compound c,signal
R1291 T2178 T2175 pobj signal,with
R1292 T2179 T2173 punct ", ",overlapped
R1293 T2180 T2173 cc but,overlapped
R1294 T2181 T2180 advmod also,but
R1295 T2182 T2173 conj was,overlapped
R1296 T2183 T2182 acomp elevated,was
R1297 T2184 T2185 advmod more,diffusely
R1298 T2185 T2182 advmod diffusely,was
R1299 T2186 T2187 punct (,4a
R1300 T2187 T2182 parataxis 4a,was
R1301 T2188 T2187 compound Fig.,4a
R1302 T2189 T2187 punct ),4a
R1303 T2190 T2173 punct .,overlapped
R1304 T2192 T2193 advmod Moreover,displayed
R1305 T2194 T2193 punct ", ",displayed
R1306 T2195 T2196 mark although,detected
R1307 T2196 T2193 advcl detected,displayed
R1308 T2197 T2198 amod multilamellar,profiles
R1309 T2198 T2196 nsubjpass profiles,detected
R1310 T2199 T2198 punct """",profiles
R1311 T2200 T2198 nmod fingerprint,profiles
R1312 T2201 T2198 punct """",profiles
R1313 T2202 T2196 auxpass were,detected
R1314 T2203 T2196 neg not,detected
R1315 T2204 T2193 punct ", ",displayed
R1316 T2205 T2206 amod confluency,aged
R1317 T2206 T2208 amod aged,cells
R1318 T2207 T2206 punct -,aged
R1319 T2208 T2193 nsubj cells,displayed
R1320 T2209 T2208 amod homozygous,cells
R1321 T2210 T2208 nmod CbCln3Δex7,cells
R1322 T2211 T2210 punct /,CbCln3Δex7
R1323 T2212 T2210 nummod 8,CbCln3Δex7
R1324 T2213 T2214 amod numerous,abnormalities
R1325 T2214 T2193 dobj abnormalities,displayed
R1326 T2215 T2214 amod ultrastructural,abnormalities
R1327 T2216 T2214 prep including,abnormalities
R1328 T2217 T2218 npadvmod electron,dense
R1329 T2218 T2219 amod dense,inclusions
R1330 T2219 T2216 pobj inclusions,including
R1331 T2220 T2219 amod characteristic,inclusions
R1332 T2221 T2220 prep of,characteristic
R1333 T2222 T2221 pobj lipofuscin,of
R1334 T2223 T2219 cc and,inclusions
R1335 T2224 T2225 amod large,autophagosomes
R1336 T2225 T2219 conj autophagosomes,inclusions
R1337 T2226 T2227 dep that,contained
R1338 T2227 T2225 relcl contained,autophagosomes
R1339 T2228 T2229 amod dense,structures
R1340 T2229 T2227 dobj structures,contained
R1341 T2230 T2229 compound core,structures
R1342 T2231 T2229 punct ", ",structures
R1343 T2232 T2233 amod degenerating,mitochondria
R1344 T2233 T2229 conj mitochondria,structures
R1345 T2234 T2233 punct ", ",mitochondria
R1346 T2235 T2233 cc and,mitochondria
R1347 T2236 T2237 amod many,vesicles
R1348 T2237 T2233 conj vesicles,mitochondria
R1349 T2238 T2237 amod smaller,vesicles
R1350 T2239 T2240 punct (,4c
R1351 T2240 T2227 parataxis 4c,contained
R1352 T2241 T2240 compound Fig.,4c
R1353 T2242 T2240 punct ),4c
R1354 T2243 T2193 punct .,displayed
R1355 T2245 T2246 compound Inclusion,bodies
R1356 T2246 T2247 nsubjpass bodies,observed
R1357 T2248 T2246 cc and,bodies
R1358 T2249 T2246 conj autophagosomes,bodies
R1359 T2250 T2247 auxpass were,observed
R1360 T2251 T2247 advmod infrequently,observed
R1361 T2252 T2247 prep in,observed
R1362 T2253 T2254 npadvmod confluency,aged
R1363 T2254 T2256 amod aged,cultures
R1364 T2255 T2254 punct -,aged
R1365 T2256 T2252 pobj cultures,in
R1366 T2257 T2258 amod wild,type
R1367 T2258 T2256 compound type,cultures
R1368 T2259 T2258 punct -,type
R1369 T2260 T2261 punct (,shown
R1370 T2261 T2247 parataxis shown,observed
R1371 T2262 T2261 nsubj data,shown
R1372 T2263 T2261 neg not,shown
R1373 T2264 T2261 punct ),shown
R1374 T2265 T2247 punct .,observed
R1375 T2526 T2527 amod Homozygous,cells
R1376 T2527 T2531 nsubj cells,process
R1377 T2528 T2527 nmod CbCln3Δex7,cells
R1378 T2529 T2528 punct /,CbCln3Δex7
R1379 T2530 T2528 nummod 8,CbCln3Δex7
R1380 T2532 T2527 cc and,cells
R1381 T2533 T2534 nmod Cln3Δex7,mice
R1382 T2534 T2527 conj mice,cells
R1383 T2535 T2533 punct /,Cln3Δex7
R1384 T2536 T2533 nummod 8,Cln3Δex7
R1385 T2537 T2534 amod knock,mice
R1386 T2538 T2537 punct -,knock
R1387 T2539 T2537 prt in,knock
R1388 T2540 T2541 compound cathepsin,D
R1389 T2541 T2531 dobj D,process
R1390 T2542 T2531 advmod abnormally,process
R1391 T2544 T2545 nsubj We,investigated
R1392 T2546 T2545 advmod next,investigated
R1393 T2547 T2548 det the,basis
R1394 T2548 T2545 dobj basis,investigated
R1395 T2549 T2548 prep for,basis
R1396 T2550 T2551 compound subunit,c
R1397 T2551 T2552 compound c,accumulation
R1398 T2552 T2549 pobj accumulation,for
R1399 T2553 T2545 punct ", ",investigated
R1400 T2554 T2545 advcl testing,investigated
R1401 T2555 T2556 det the,hypothesis
R1402 T2556 T2554 dobj hypothesis,testing
R1403 T2557 T2558 mark that,is
R1404 T2558 T2556 acl is,hypothesis
R1405 T2559 T2560 compound cathepsin,D
R1406 T2560 T2558 nsubj D,is
R1407 T2561 T2558 acomp abnormal,is
R1408 T2562 T2563 mark since,is
R1409 T2563 T2558 advcl is,is
R1410 T2564 T2563 nsubj it,is
R1411 T2565 T2563 acomp required,is
R1412 T2566 T2565 prep for,required
R1413 T2567 T2568 compound ATP,degradation
R1414 T2568 T2566 pobj degradation,for
R1415 T2569 T2568 compound synthase,degradation
R1416 T2570 T2571 compound subunit,c
R1417 T2571 T2568 compound c,degradation
R1418 T2572 T2568 prep in,degradation
R1419 T2573 T2574 det the,lysosome
R1420 T2574 T2572 pobj lysosome,in
R1421 T2575 T2576 punct [,13
R1422 T2576 T2554 parataxis 13,testing
R1423 T2577 T2576 punct ],13
R1424 T2578 T2545 punct .,investigated
R1425 T2580 T2581 nsubj We,tested
R1426 T2582 T2581 advmod first,tested
R1427 T2583 T2584 compound cathepsin,D
R1428 T2584 T2581 dobj D,tested
R1429 T2585 T2584 appos transport,D
R1430 T2586 T2585 cc and,transport
R1431 T2587 T2585 conj processing,transport
R1432 T2588 T2581 prep in,tested
R1433 T2589 T2590 amod homozygous,cells
R1434 T2590 T2588 pobj cells,in
R1435 T2591 T2590 nmod CbCln3Δex7,cells
R1436 T2592 T2591 punct /,CbCln3Δex7
R1437 T2593 T2591 nummod 8,CbCln3Δex7
R1438 T2594 T2590 cc and,cells
R1439 T2595 T2596 nmod Cln3Δex7,mice
R1440 T2596 T2590 conj mice,cells
R1441 T2597 T2595 punct /,Cln3Δex7
R1442 T2598 T2595 nummod 8,Cln3Δex7
R1443 T2599 T2581 advcl using,tested
R1444 T2600 T2601 amod anti-cathepsin,D
R1445 T2601 T2602 amod D,antibody
R1446 T2602 T2599 dobj antibody,using
R1447 T2603 T2604 dep that,recognizes
R1448 T2604 T2602 relcl recognizes,antibody
R1449 T2605 T2606 amod unprocessed,isoforms
R1450 T2606 T2604 dobj isoforms,recognizes
R1451 T2607 T2605 cc and,unprocessed
R1452 T2608 T2605 conj processed,unprocessed
R1453 T2609 T2610 compound cathepsin,D
R1454 T2610 T2606 compound D,isoforms
R1455 T2611 T2581 punct .,tested
R1456 T2613 T2614 nsubj Immunostaining,revealed
R1457 T2615 T2613 prep of,Immunostaining
R1458 T2616 T2617 amod wild,type
R1459 T2617 T2619 nmod type,cells
R1460 T2618 T2617 punct -,type
R1461 T2619 T2615 pobj cells,of
R1462 T2620 T2617 cc and,type
R1463 T2621 T2617 conj homozygous,type
R1464 T2622 T2619 nmod CbCln3Δex7,cells
R1465 T2623 T2622 punct /,CbCln3Δex7
R1466 T2624 T2622 nummod 8,CbCln3Δex7
R1467 T2625 T2626 det a,distribution
R1468 T2626 T2614 dobj distribution,revealed
R1469 T2627 T2626 amod perinuclear,distribution
R1470 T2628 T2627 cc and,perinuclear
R1471 T2629 T2627 conj punctate,perinuclear
R1472 T2630 T2626 amod vesicular,distribution
R1473 T2631 T2632 compound cathepsin,D
R1474 T2632 T2626 compound D,distribution
R1475 T2633 T2614 punct ", ",revealed
R1476 T2634 T2614 advcl consistent,revealed
R1477 T2635 T2634 prep with,consistent
R1478 T2636 T2637 poss its,transport
R1479 T2637 T2635 pobj transport,with
R1480 T2638 T2637 cc and,transport
R1481 T2639 T2637 conj processing,transport
R1482 T2640 T2637 prep through,transport
R1483 T2641 T2642 det the,pathway
R1484 T2642 T2640 pobj pathway,through
R1485 T2643 T2642 amod secretory,pathway
R1486 T2644 T2637 cc and,transport
R1487 T2645 T2637 conj delivery,transport
R1488 T2646 T2645 prep to,delivery
R1489 T2647 T2648 det the,lysosome
R1490 T2648 T2646 pobj lysosome,to
R1491 T2649 T2650 punct (,5a
R1492 T2650 T2614 parataxis 5a,revealed
R1493 T2651 T2650 compound Fig.,5a
R1494 T2652 T2650 punct ),5a
R1495 T2653 T2614 punct .,revealed
R1496 T2655 T2656 advmod However,was
R1497 T2657 T2656 punct ", ",was
R1498 T2658 T2656 prep in,was
R1499 T2659 T2660 amod homozygous,cells
R1500 T2660 T2658 pobj cells,in
R1501 T2661 T2660 nmod CbCln3Δex7,cells
R1502 T2662 T2661 punct /,CbCln3Δex7
R1503 T2663 T2661 nummod 8,CbCln3Δex7
R1504 T2664 T2656 punct ", ",was
R1505 T2665 T2666 compound cathepsin,D
R1506 T2666 T2667 compound D,distribution
R1507 T2667 T2656 nsubj distribution,was
R1508 T2668 T2669 advmod less,vesicular
R1509 T2669 T2656 acomp vesicular,was
R1510 T2670 T2669 cc and,vesicular
R1511 T2671 T2672 advmod more,clustered
R1512 T2672 T2669 conj clustered,vesicular
R1513 T2673 T2672 amod perinuclear,clustered
R1514 T2674 T2672 punct -,clustered
R1515 T2675 T2669 prep than,vesicular
R1516 T2676 T2675 prep in,than
R1517 T2677 T2678 amod wild,type
R1518 T2678 T2680 compound type,cells
R1519 T2679 T2678 punct -,type
R1520 T2680 T2676 pobj cells,in
R1521 T2681 T2656 punct .,was
R1522 T2683 T2684 nsubj Immunoblots,showed
R1523 T2685 T2683 prep of,Immunoblots
R1524 T2686 T2687 amod homozygous,extracts
R1525 T2687 T2685 pobj extracts,of
R1526 T2688 T2689 nmod CbCln3Δex7,cell
R1527 T2689 T2687 nmod cell,extracts
R1528 T2690 T2688 punct /,CbCln3Δex7
R1529 T2691 T2688 nummod 8,CbCln3Δex7
R1530 T2692 T2689 cc and,cell
R1531 T2693 T2694 nmod Cln3Δex7,tissue
R1532 T2694 T2689 conj tissue,cell
R1533 T2695 T2693 punct /,Cln3Δex7
R1534 T2696 T2693 nummod 8,Cln3Δex7
R1535 T2697 T2684 advmod also,showed
R1536 T2698 T2699 amod altered,levels
R1537 T2699 T2684 dobj levels,showed
R1538 T2700 T2699 amod relative,levels
R1539 T2701 T2699 prep of,levels
R1540 T2702 T2703 compound cathepsin,D
R1541 T2703 T2704 compound D,isoforms
R1542 T2704 T2701 pobj isoforms,of
R1543 T2705 T2706 punct (,5b
R1544 T2706 T2684 parataxis 5b,showed
R1545 T2707 T2706 compound Fig.,5b
R1546 T2708 T2706 punct ),5b
R1547 T2709 T2684 punct .,showed
R1548 T2711 T2712 compound Cathepsin,D
R1549 T2712 T2713 compound D,isoforms
R1550 T2713 T2714 nsubj isoforms,represent
R1551 T2715 T2713 punct ", ",isoforms
R1552 T2716 T2713 acl identified,isoforms
R1553 T2717 T2716 agent by,identified
R1554 T2718 T2719 amod relative,weights
R1555 T2719 T2717 pobj weights,by
R1556 T2720 T2719 amod molecular,weights
R1557 T2721 T2714 punct ", ",represent
R1558 T2722 T2723 det the,precursor
R1559 T2723 T2714 dobj precursor,represent
R1560 T2724 T2725 punct ~,45
R1561 T2725 T2726 nummod 45,kDa
R1562 T2726 T2723 compound kDa,precursor
R1563 T2727 T2723 punct ", ",precursor
R1564 T2728 T2729 det the,form
R1565 T2729 T2723 conj form,precursor
R1566 T2730 T2731 punct ~,43
R1567 T2731 T2732 nummod 43,kDa
R1568 T2732 T2729 nmod kDa,form
R1569 T2733 T2729 amod intermediate,form
R1570 T2734 T2735 amod single,chain
R1571 T2735 T2729 compound chain,form
R1572 T2736 T2729 prep of,form
R1573 T2737 T2738 det the,enzyme
R1574 T2738 T2736 pobj enzyme,of
R1575 T2739 T2729 punct ", ",form
R1576 T2740 T2729 cc and,form
R1577 T2741 T2742 det the,chain
R1578 T2742 T2729 conj chain,form
R1579 T2743 T2744 nummod 31,kDa
R1580 T2744 T2742 nmod kDa,chain
R1581 T2745 T2742 amod heavy,chain
R1582 T2746 T2742 prep of,chain
R1583 T2747 T2748 det the,form
R1584 T2748 T2746 pobj form,of
R1585 T2749 T2750 amod double,chain
R1586 T2750 T2748 compound chain,form
R1587 T2751 T2750 punct -,chain
R1588 T2752 T2748 prep of,form
R1589 T2753 T2754 det the,enzyme
R1590 T2754 T2752 pobj enzyme,of
R1591 T2755 T2754 amod mature,enzyme
R1592 T2756 T2757 punct [,14
R1593 T2757 T2714 parataxis 14,represent
R1594 T2758 T2757 punct ],14
R1595 T2759 T2714 punct .,represent
R1596 T2761 T2762 prep In,reduced
R1597 T2763 T2764 amod homozygous,extracts
R1598 T2764 T2761 pobj extracts,In
R1599 T2765 T2766 nmod CbCln3Δex7,cell
R1600 T2766 T2764 nmod cell,extracts
R1601 T2767 T2765 punct /,CbCln3Δex7
R1602 T2768 T2765 nummod 8,CbCln3Δex7
R1603 T2769 T2766 cc and,cell
R1604 T2770 T2771 nmod Cln3Δex7,tissue
R1605 T2771 T2766 conj tissue,cell
R1606 T2772 T2770 punct /,Cln3Δex7
R1607 T2773 T2770 nummod 8,Cln3Δex7
R1608 T2774 T2762 punct ", ",reduced
R1609 T2775 T2776 det the,precursor
R1610 T2776 T2762 nsubjpass precursor,reduced
R1611 T2777 T2776 cc and,precursor
R1612 T2778 T2779 amod heavy,chains
R1613 T2779 T2776 conj chains,precursor
R1614 T2780 T2762 auxpass were,reduced
R1615 T2781 T2762 punct ", ",reduced
R1616 T2782 T2762 cc and,reduced
R1617 T2783 T2784 det the,chain
R1618 T2784 T2786 nsubj chain,was
R1619 T2785 T2784 amod single,chain
R1620 T2786 T2762 conj was,reduced
R1621 T2787 T2788 advmod slightly,elevated
R1622 T2788 T2786 acomp elevated,was
R1623 T2789 T2786 prep compared,was
R1624 T2790 T2789 prep to,compared
R1625 T2791 T2792 amod wild,type
R1626 T2792 T2794 compound type,extracts
R1627 T2793 T2792 punct -,type
R1628 T2794 T2790 pobj extracts,to
R1629 T2795 T2786 punct .,was
R1630 T2797 T2798 det The,media
R1631 T2798 T2801 nsubj media,contain
R1632 T2799 T2800 amod cellular,growth
R1633 T2800 T2798 compound growth,media
R1634 T2802 T2801 aux did,contain
R1635 T2803 T2801 neg not,contain
R1636 T2804 T2805 amod altered,levels
R1637 T2805 T2801 dobj levels,contain
R1638 T2806 T2805 prep of,levels
R1639 T2807 T2808 compound cathepsin,D
R1640 T2808 T2806 pobj D,of
R1641 T2809 T2801 punct ", ",contain
R1642 T2810 T2801 advcl indicating,contain
R1700 T2892 T2890 pobj turnover,for
R1701 T2893 T2894 compound subunit,c
R1702 T2894 T2892 compound c,turnover
R1703 T2895 T2874 punct .,tested
R1704 T2897 T2898 prep In,altered
R1705 T2899 T2900 det a,assay
R1706 T2900 T2897 pobj assay,In
R1707 T2901 T2900 amod fluorogenic,assay
R1708 T2902 T2903 advmod in,vitro
R1709 T2903 T2900 amod vitro,assay
R1710 T2904 T2898 punct ", ",altered
R1711 T2905 T2906 compound cathepsin,D
R1712 T2906 T2907 compound D,activity
R1713 T2907 T2898 nsubjpass activity,altered
R1714 T2908 T2907 prep in,activity
R1715 T2909 T2910 amod total,extracts
R1716 T2910 T2908 pobj extracts,in
R1717 T2911 T2910 amod cellular,extracts
R1718 T2912 T2898 auxpass was,altered
R1719 T2913 T2898 neg not,altered
R1720 T2914 T2898 advmod significantly,altered
R1721 T2915 T2898 prep in,altered
R1722 T2916 T2917 amod homozygous,cells
R1723 T2917 T2915 pobj cells,in
R1724 T2918 T2917 nmod CbCln3Δex7,cells
R1725 T2919 T2918 punct /,CbCln3Δex7
R1726 T2920 T2918 nummod 8,CbCln3Δex7
R1727 T2921 T2922 punct (,RFU
R1728 T2922 T2917 parataxis RFU,cells
R1729 T2923 T2924 quantmod 376,89
R1730 T2924 T2922 nummod 89,RFU
R1731 T2925 T2924 punct ±,89
R1732 T2926 T2927 punct /,protein
R1733 T2927 T2922 prep protein,RFU
R1734 T2928 T2927 nmod μg,protein
R1735 T2929 T2927 amod total,protein
R1736 T2930 T2922 punct ),RFU
R1737 T2931 T2917 punct ", ",cells
R1738 T2932 T2917 cc versus,cells
R1739 T2933 T2934 amod wild,type
R1740 T2934 T2936 compound type,cells
R1741 T2935 T2934 punct -,type
R1742 T2936 T2917 conj cells,cells
R1743 T2937 T2938 punct (,RFU
R1744 T2938 T2936 parataxis RFU,cells
R1745 T2939 T2940 quantmod 324,58
R1746 T2940 T2938 nummod 58,RFU
R1747 T2941 T2940 quantmod ±,58
R1748 T2942 T2943 punct /,protein
R1749 T2943 T2938 prep protein,RFU
R1750 T2944 T2943 nmod μg,protein
R1751 T2945 T2943 amod total,protein
R1752 T2946 T2938 punct ),RFU
R1753 T2947 T2898 punct ", ",altered
R1754 T2948 T2949 mark although,observed
R1755 T2949 T2898 advcl observed,altered
R1756 T2950 T2951 det a,trend
R1757 T2951 T2949 nsubjpass trend,observed
R1758 T2952 T2951 amod consistent,trend
R1759 T2953 T2951 prep towards,trend
R1760 T2954 T2955 amod increased,activity
R1761 T2955 T2953 pobj activity,towards
R1762 T2956 T2955 amod enzymatic,activity
R1763 T2957 T2955 prep in,activity
R1764 T2958 T2959 compound mutant,cells
R1765 T2959 T2957 pobj cells,in
R1766 T2960 T2949 auxpass was,observed
R1767 T2961 T2898 punct .,altered
R1768 T2963 T2964 advmod Thus,disrupted
R1769 T2965 T2964 punct ", ",disrupted
R1770 T2966 T2967 compound cathepsin,D
R1771 T2967 T2964 nsubjpass D,disrupted
R1772 T2968 T2967 appos transport,D
R1773 T2969 T2968 cc and,transport
R1774 T2970 T2968 conj processing,transport
R1775 T2971 T2964 auxpass are,disrupted
R1776 T2972 T2964 prep in,disrupted
R1777 T2973 T2974 amod homozygous,cells
R1778 T2974 T2972 pobj cells,in
R1779 T2975 T2974 nmod CbCln3Δex7,cells
R1780 T2976 T2975 punct /,CbCln3Δex7
R1781 T2977 T2975 nummod 8,CbCln3Δex7
R1782 T2978 T2964 prep in,disrupted
R1783 T2979 T2980 det a,manner
R1784 T2980 T2978 pobj manner,in
R1785 T2981 T2980 amod such,manner
R1786 T2982 T2983 mark that,appears
R1787 T2983 T2981 ccomp appears,such
R1788 T2984 T2985 amod enzymatic,activity
R1789 T2985 T2983 nsubj activity,appears
R1790 T2986 T2987 aux to,be
R1791 T2987 T2983 xcomp be,appears
R1792 T2988 T2989 advmod relatively,unaffected
R1793 T2989 T2987 acomp unaffected,be
R1794 T2990 T2964 punct .,disrupted
R1795 T3349 T3350 amod Homozygous,cells
R1796 T3350 T3354 nsubj cells,show
R1797 T3351 T3350 nmod CbCln3Δex7,cells
R1798 T3352 T3351 punct /,CbCln3Δex7
R1799 T3353 T3351 nummod 8,CbCln3Δex7
R1800 T3355 T3356 amod abnormal,organelles
R1801 T3356 T3354 dobj organelles,show
R1802 T3357 T3356 compound membrane,organelles
R1803 T3359 T3360 det The,transport
R1804 T3360 T3362 nsubj transport,suggested
R1805 T3361 T3360 amod abnormal,transport
R1806 T3362 T3368 ccomp suggested,surveyed
R1807 T3363 T3360 cc and,transport
R1808 T3364 T3360 conj processing,transport
R1809 T3365 T3360 prep of,transport
R1810 T3366 T3367 compound cathepsin,D
R1811 T3367 T3365 pobj D,of
R1812 T3369 T3370 compound membrane,trafficking
R1813 T3370 T3371 compound trafficking,disruptions
R1814 T3371 T3362 dobj disruptions,suggested
R1815 T3372 T3362 prep in,suggested
R1816 T3373 T3374 amod homozygous,cells
R1817 T3374 T3372 pobj cells,in
R1818 T3375 T3374 nmod CbCln3Δex7,cells
R1819 T3376 T3375 punct /,CbCln3Δex7
R1820 T3377 T3375 nummod 8,CbCln3Δex7
R1821 T3378 T3368 punct ;,surveyed
R1822 T3379 T3368 advmod therefore,surveyed
R1823 T3380 T3368 punct ", ",surveyed
R1824 T3381 T3368 nsubj we,surveyed
R1825 T3382 T3383 det the,distribution
R1826 T3383 T3368 dobj distribution,surveyed
R1827 T3384 T3383 amod subcellular,distribution
R1828 T3385 T3383 cc and,distribution
R1829 T3386 T3383 conj morphology,distribution
R1830 T3387 T3383 prep of,distribution
R1831 T3388 T3389 compound membrane,organelles
R1832 T3389 T3387 pobj organelles,of
R1833 T3390 T3368 punct .,surveyed
R1834 T3392 T3393 nsubj Components,appear
R1835 T3394 T3392 prep of,Components
R1836 T3395 T3396 det the,pathway
R1837 T3396 T3394 pobj pathway,of
R1838 T3397 T3396 amod secretory,pathway
R1839 T3398 T3392 punct ", ",Components
R1840 T3399 T3392 prep including,Components
R1841 T3400 T3401 det the,ER
R1842 T3401 T3399 pobj ER,including
R1843 T3402 T3401 punct ", ",ER
R1844 T3403 T3404 compound cis,Golgi
R1845 T3404 T3401 conj Golgi,ER
R1846 T3405 T3404 punct -,Golgi
R1847 T3406 T3404 punct ", ",Golgi
R1848 T3407 T3404 cc and,Golgi
R1849 T3408 T3409 compound trans,Golgi
R1850 T3409 T3404 conj Golgi,Golgi
R1851 T3410 T3409 punct -,Golgi
R1852 T3411 T3393 punct ", ",appear
R1853 T3412 T3393 aux did,appear
R1854 T3413 T3393 neg not,appear
R1855 T3414 T3393 oprd altered,appear
R1856 T3415 T3414 prep from,altered
R1857 T3416 T3417 amod wild,type
R1858 T3417 T3419 compound type,appearance
R1859 T3418 T3417 punct -,type
R1860 T3419 T3415 pobj appearance,from
R1861 T3420 T3421 advmod when,labeled
R1862 T3421 T3393 advcl labeled,appear
R1863 T3422 T3421 prep with,labeled
R1864 T3423 T3424 det the,markers
R1865 T3424 T3422 pobj markers,with
R1866 T3425 T3424 amod respective,markers
R1867 T3426 T3424 punct ", ",markers
R1868 T3427 T3428 compound protein,isomerase
R1869 T3428 T3424 appos isomerase,markers
R1870 T3429 T3428 compound disulfide,isomerase
R1871 T3430 T3428 punct (,isomerase
R1872 T3431 T3428 appos PDI,isomerase
R1873 T3432 T3428 punct ),isomerase
R1874 T3433 T3428 punct ", ",isomerase
R1875 T3434 T3428 conj GM130,isomerase
R1876 T3435 T3434 punct ", ",GM130
R1877 T3436 T3434 cc and,GM130
R1878 T3437 T3434 conj VVL,GM130
R1879 T3438 T3439 punct (,shown
R1880 T3439 T3393 parataxis shown,appear
R1881 T3440 T3439 nsubj data,shown
R1882 T3441 T3439 neg not,shown
R1883 T3442 T3439 punct ),shown
R1884 T3443 T3393 punct .,appear
R1885 T3445 T3446 prep By,had
R1886 T3447 T3445 pobj contrast,By
R1887 T3448 T3446 punct ", ",had
R1888 T3449 T3450 det the,markers
R1889 T3450 T3446 nsubj markers,had
R1890 T3451 T3450 amod lysosomal,markers
R1891 T3452 T3450 punct ", ",markers
R1892 T3453 T3450 appos Lysotracker,markers
R1893 T3454 T3453 cc and,Lysotracker
R1894 T3455 T3453 conj Lamp,Lysotracker
R1895 T3456 T3455 nummod 2,Lamp
R1896 T3457 T3458 advmod significantly,altered
R1897 T3458 T3459 amod altered,signal
R1898 T3459 T3446 dobj signal,had
R1899 T3460 T3446 prep in,had
R1900 T3461 T3462 amod homozygous,cells
R1901 T3462 T3460 pobj cells,in
R1902 T3463 T3462 nmod CbCln3Δex7,cells
R1903 T3464 T3463 punct /,CbCln3Δex7
R1904 T3465 T3463 nummod 8,CbCln3Δex7
R1905 T3466 T3462 punct ", ",cells
R1906 T3467 T3462 cc versus,cells
R1907 T3468 T3469 amod wild,type
R1908 T3469 T3471 compound type,cells
R1909 T3470 T3469 punct -,type
R1910 T3471 T3462 conj cells,cells
R1911 T3472 T3446 punct .,had
R1912 T3474 T3475 amod Wild,type
R1913 T3475 T3477 compound type,cells
R1914 T3476 T3475 punct -,type
R1915 T3477 T3478 nsubj cells,exhibited
R1916 T3479 T3480 advmod brightly,stained
R1917 T3480 T3481 amod stained,lysosomes
R1918 T3481 T3478 dobj lysosomes,exhibited
R1919 T3482 T3483 dep that,were
R1920 T3483 T3481 relcl were,lysosomes
R1921 T3484 T3483 acomp large,were
R1922 T3485 T3484 cc and,large
R1923 T3486 T3484 conj clustered,large
R1924 T3487 T3483 prep in,were
R1925 T3488 T3489 det the,region
R1926 T3489 T3487 pobj region,in
R1927 T3490 T3489 amod perinuclear,region
R1928 T3491 T3492 mark whereas,were
R1929 T3492 T3483 advcl were,were
R1930 T3493 T3494 amod homozygous,lysosomes
R1931 T3494 T3492 nsubj lysosomes,were
R1932 T3495 T3494 nmod CbCln3Δex7,lysosomes
R1933 T3496 T3495 punct /,CbCln3Δex7
R1934 T3497 T3495 nummod 8,CbCln3Δex7
R1935 T3498 T3499 advmod lightly,stained
R1936 T3499 T3500 amod stained,vesicles
R1937 T3500 T3492 attr vesicles,were
R1938 T3501 T3500 punct ", ",vesicles
R1939 T3502 T3500 amod smaller,vesicles
R1940 T3503 T3504 dep that,scattered
R1941 T3504 T3500 relcl scattered,vesicles
R1942 T3505 T3504 auxpass were,scattered
R1943 T3506 T3507 advmod more,diffusely
R1944 T3507 T3504 advmod diffusely,scattered
R1945 T3508 T3504 prep in,scattered
R1946 T3509 T3510 det the,cytoplasm
R1947 T3510 T3508 pobj cytoplasm,in
R1948 T3511 T3510 prep of,cytoplasm
R1949 T3512 T3513 det the,cell
R1950 T3513 T3511 pobj cell,of
R1951 T3514 T3515 punct (,Fig.
R1952 T3515 T3478 parataxis Fig.,exhibited
R1953 T3516 T3515 nummod 6,Fig.
R1954 T3517 T3515 punct ),Fig.
R1955 T3518 T3478 punct .,exhibited
R1956 T3520 T3521 nmod Lamp,distribution
R1957 T3521 T3523 nsubjpass distribution,altered
R1958 T3522 T3520 nummod 1,Lamp
R1959 T3524 T3523 auxpass was,altered
R1960 T3525 T3523 advmod also,altered
R1961 T3526 T3523 punct ", ",altered
R1962 T3527 T3528 mark as,noted
R1963 T3528 T3523 advcl noted,altered
R1964 T3529 T3528 advmod previously,noted
R1965 T3530 T3531 punct (,Fig.
R1966 T3531 T3523 parataxis Fig.,altered
R1967 T3532 T3531 nummod 3,Fig.
R1968 T3533 T3531 punct ),Fig.
R1969 T3534 T3523 punct .,altered
R1970 T3536 T3537 advmod However,were
R1971 T3538 T3537 punct ", ",were
R1972 T3539 T3540 nmod Lamp,levels
R1973 T3540 T3537 nsubj levels,were
R1974 T3541 T3539 nummod 1,Lamp
R1975 T3542 T3539 cc and,Lamp
R1976 T3543 T3539 conj Lamp,Lamp
R1977 T3544 T3543 nummod 2,Lamp
R1978 T3545 T3546 amod total,protein
R1979 T3546 T3540 compound protein,levels
R1980 T3547 T3537 acomp similar,were
R1981 T3548 T3537 prep in,were
R1982 T3549 T3550 amod wild,type
R1984 T3551 T3550 punct -,type
R1992 T3559 T3560 compound immunoblot,analysis
R1993 T3560 T3558 pobj analysis,by
R1994 T3561 T3537 punct ", ",were
R1995 T3562 T3537 advcl indicating,were
R1996 T3563 T3564 det the,signal
R1997 T3564 T3566 nsubj signal,reflects
R1998 T3565 T3564 amod altered,signal
R1999 T3566 T3562 advcl reflects,indicating
R2000 T3567 T3566 advmod likely,reflects
R2001 T3568 T3569 amod dispersed,lysosomes
R2002 T3569 T3566 dobj lysosomes,reflects
R2003 T3570 T3569 cc or,lysosomes
R2004 T3571 T3572 amod altered,localization
R2005 T3572 T3569 conj localization,lysosomes
R2006 T3573 T3572 cc and,localization
R2007 T3574 T3572 punct /,localization
R2008 T3575 T3572 cc or,localization
R2009 T3576 T3577 compound epitope,availability
R2010 T3577 T3572 conj availability,localization
R2011 T3578 T3579 punct (,shown
R2012 T3579 T3562 parataxis shown,indicating
R2013 T3580 T3579 nsubj data,shown
R2014 T3581 T3579 neg not,shown
R2015 T3582 T3579 punct ),shown
R2016 T3583 T3537 punct .,were
R2017 T3585 T3586 nsubj It,is
R2018 T3587 T3586 acomp noteworthy,is
R2019 T3588 T3589 mark that,exhibited
R2020 T3589 T3586 ccomp exhibited,is
R2021 T3590 T3591 compound Lysotracker,dye
R2022 T3591 T3589 nsubj dye,exhibited
R2023 T3592 T3591 punct ", ",dye
R2024 T3593 T3594 dep which,accumulates
R2025 T3594 T3591 relcl accumulates,dye
R2026 T3595 T3594 advmod selectively,accumulates
R2027 T3596 T3594 prep in,accumulates
R2028 T3597 T3598 amod acidic,compartments
R2029 T3598 T3596 pobj compartments,in
R2030 T3599 T3589 punct ", ",exhibited
R2031 T3600 T3601 det the,reduction
R2032 T3601 T3589 dobj reduction,exhibited
R2033 T3602 T3603 advmod most,marked
R2034 T3603 T3601 amod marked,reduction
R2035 T3604 T3601 prep in,reduction
R2036 T3605 T3606 amod lysosomal,labeling
R2037 T3606 T3604 pobj labeling,in
R2038 T3607 T3586 punct .,is
R2039 T3609 T3610 det This,observation
R2040 T3610 T3611 nsubj observation,reflect
R2041 T3612 T3611 aux may,reflect
R2042 T3613 T3614 amod altered,pH
R2043 T3614 T3611 dobj pH,reflect
R2044 T3615 T3614 amod lysosomal,pH
R2045 T3616 T3614 punct ", ",pH
R2046 T3617 T3618 det an,finding
R2047 T3618 T3614 appos finding,pH
R2048 T3619 T3618 amod established,finding
R2049 T3620 T3618 prep in,finding
R2050 T3621 T3620 pobj JNCL,in
R2051 T3622 T3623 punct [,15
R2052 T3623 T3611 parataxis 15,reflect
R2053 T3624 T3623 nummod 10,15
R2054 T3625 T3623 punct ",",15
R2055 T3626 T3623 punct ],15
R2056 T3627 T3611 punct .,reflect
R2079 T3683 T3685 compound phase,endocytosis
R2080 T3684 T3683 punct -,phase
R2081 T3685 T3667 nsubjpass endocytosis,altered
R2082 T3686 T3667 auxpass was,altered
R2083 T3687 T3667 advmod also,altered
R2084 T3688 T3667 prep in,altered
R2085 T3689 T3690 amod homozygous,cells
R2086 T3690 T3688 pobj cells,in
R2087 T3691 T3690 nmod CbCln3Δex7,cells
R2088 T3692 T3691 punct /,CbCln3Δex7
R2089 T3693 T3691 nummod 8,CbCln3Δex7
R2090 T3694 T3667 punct ", ",altered
R2091 T3695 T3696 mark as,measured
R2092 T3696 T3667 advcl measured,altered
R2093 T3697 T3696 agent by,measured
R2094 T3698 T3699 compound dextran,FITC
R2095 T3699 T3701 compound FITC,uptake
R2096 T3700 T3699 punct -,FITC
R2097 T3701 T3697 pobj uptake,by
R2098 T3702 T3703 punct (,Fig.
R2099 T3703 T3667 parataxis Fig.,altered
R2100 T3704 T3703 nummod 7,Fig.
R2101 T3705 T3703 punct ),Fig.
R2102 T3706 T3667 punct .,altered
R2103 T3708 T3709 prep Following,displayed
R2104 T3710 T3711 det a,incubation
R2105 T3711 T3708 pobj incubation,Following
R2106 T3712 T3713 nummod 15,minute
R2107 T3713 T3711 compound minute,incubation
R2108 T3714 T3713 punct -,minute
R2109 T3715 T3711 prep in,incubation
R2110 T3716 T3715 pobj media,in
R2111 T3717 T3716 acl containing,media
R2112 T3718 T3719 compound dextran,FITC
R2113 T3719 T3717 dobj FITC,containing
R2114 T3720 T3719 punct -,FITC
R2115 T3721 T3709 punct ", ",displayed
R2116 T3722 T3723 amod wild,type
R2117 T3723 T3725 nmod type,cells
R2118 T3724 T3723 punct -,type
R2119 T3725 T3709 nsubj cells,displayed
R2120 T3726 T3723 cc and,type
R2121 T3727 T3723 conj heterozygote,type
R2122 T3728 T3729 advmod brightly,stained
R2123 T3729 T3730 amod stained,vesicles
R2124 T3730 T3709 dobj vesicles,displayed
R2125 T3731 T3730 punct ", ",vesicles
R2126 T3732 T3730 amod large,vesicles
R2127 T3733 T3730 amod endocytic,vesicles
R2128 T3734 T3735 dep that,clustered
R2129 T3735 T3730 relcl clustered,vesicles
R2130 T3736 T3735 auxpass were,clustered
R2131 T3737 T3735 prep in,clustered
R2132 T3738 T3739 det the,region
R2133 T3739 T3737 pobj region,in
R2134 T3740 T3739 amod perinuclear,region
R2135 T3741 T3709 punct .,displayed
R2136 T3743 T3744 advmod However,stained
R2137 T3745 T3744 punct ", ",stained
R2138 T3746 T3747 amod homozygous,cells
R2139 T3747 T3744 nsubjpass cells,stained
R2140 T3748 T3747 nmod CbCln3Δex7,cells
R2141 T3749 T3748 punct /,CbCln3Δex7
R2142 T3750 T3748 nummod 8,CbCln3Δex7
R2143 T3751 T3744 auxpass were,stained
R2144 T3752 T3753 advmod less,brightly
R2145 T3753 T3744 advmod brightly,stained
R2146 T3754 T3744 prep with,stained
R2147 T3755 T3756 amod most,signal
R2148 T3756 T3760 nsubj signal,localizing
R2149 T3757 T3758 compound dextran,FITC
R2150 T3758 T3756 compound FITC,signal
R2151 T3759 T3758 punct -,FITC
R2152 T3760 T3754 pcomp localizing,with
R2153 T3761 T3760 prep to,localizing
R2154 T3762 T3763 amod smaller,vesicles
R2155 T3763 T3761 pobj vesicles,to
R2156 T3764 T3763 acl scattered,vesicles
R2157 T3765 T3764 prep throughout,scattered
R2158 T3766 T3767 det the,cytoplasm
R2159 T3767 T3765 pobj cytoplasm,throughout
R2187 T3829 T3824 appos morphology,CbCln3Δex7
R2188 T3830 T3829 cc and,morphology
R2189 T3831 T3829 conj function,morphology
R2190 T3832 T3806 punct .,analyzed
R2191 T3834 T3835 amod Mitochondrial,distribution
R2192 T3835 T3836 nsubj distribution,was
R2193 T3836 T3843 ccomp was,appeared
R2194 T3837 T3835 prep in,distribution
R2195 T3838 T3839 amod homozygous,cells
R2196 T3839 T3837 pobj cells,in
R2197 T3840 T3839 nmod CbCln3Δex7,cells
R2198 T3841 T3840 punct /,CbCln3Δex7
R2199 T3842 T3840 nummod 8,CbCln3Δex7
R2200 T3844 T3836 acomp indistinguishable,was
R2201 T3845 T3844 prep from,indistinguishable
R2202 T3846 T3847 amod wild,type
R2203 T3847 T3849 nmod type,cells
R2204 T3848 T3847 punct -,type
R2205 T3849 T3845 pobj cells,from
R2206 T3850 T3847 cc and,type
R2207 T3851 T3847 conj heterozygous,type
R2208 T3852 T3843 punct ;,appeared
R2209 T3853 T3843 advmod however,appeared
R2210 T3854 T3843 punct ", ",appeared
R2211 T3855 T3856 amod homozygous,mitochondria
R2212 T3856 T3843 nsubj mitochondria,appeared
R2213 T3857 T3856 nmod CbCln3Δex7,mitochondria
R2214 T3858 T3857 punct /,CbCln3Δex7
R2215 T3859 T3857 nummod 8,CbCln3Δex7
R2216 T3860 T3861 advmod more,elongated
R2217 T3861 T3843 oprd elongated,appeared
R2218 T3862 T3861 prep by,elongated
R2219 T3863 T3864 compound grp75,immunostaining
R2220 T3864 T3862 pobj immunostaining,by
R2221 T3865 T3864 compound marker,immunostaining
R2222 T3866 T3864 cc and,immunostaining
R2223 T3867 T3868 compound TEM,analysis
R2224 T3868 T3864 conj analysis,immunostaining
R2225 T3869 T3870 punct (,8a
R2226 T3870 T3843 parataxis 8a,appeared
R2227 T3871 T3870 compound Fig.,8a
R2228 T3872 T3870 punct ),8a
R2229 T3873 T3843 punct .,appeared
R2230 T3875 T3876 nummod 72,%
R2231 T3876 T3877 nsubj %,were
R2232 T3878 T3876 prep of,%
R2233 T3879 T3880 amod homozygous,mitochondria
R2234 T3880 T3878 pobj mitochondria,of
R2235 T3881 T3880 compound mutant,mitochondria
R2236 T3882 T3883 amod greater,0.6
R2237 T3883 T3885 nummod 0.6,μm
R2238 T3884 T3883 quantmod than,0.6
R2239 T3885 T3877 attr μm,were
R2240 T3886 T3885 prep in,μm
R2241 T3887 T3886 pobj length,in
R2242 T3888 T3889 punct (,μm
R2243 T3889 T3877 parataxis μm,were
R2244 T3890 T3889 nsubj range,μm
R2245 T3891 T3889 punct =,μm
R2246 T3892 T3889 nummod 0.26,μm
R2247 T3893 T3889 prep to,μm
R2248 T3894 T3895 nummod 2.75,μm
R2249 T3895 T3893 pobj μm,to
R2250 T3896 T3889 punct ),μm
R2251 T3897 T3877 punct ", ",were
R2252 T3898 T3899 mark while,reached
R2253 T3899 T3877 advcl reached,were
R2254 T3900 T3901 amod fewer,mitochondria
R2255 T3901 T3899 nsubj mitochondria,reached
R2256 T3902 T3903 amod wild,type
R2257 T3903 T3901 compound type,mitochondria
R2258 T3904 T3903 punct -,type
R2259 T3905 T3906 punct (,%
R2260 T3906 T3901 parataxis %,mitochondria
R2261 T3907 T3906 nummod 51,%
R2262 T3908 T3906 punct ),%
R2263 T3909 T3910 det this,length
R2264 T3910 T3899 dobj length,reached
R2265 T3911 T3912 punct (,μm
R2266 T3912 T3899 parataxis μm,reached
R2267 T3913 T3912 nsubj range,μm
R2268 T3914 T3912 punct =,μm
R2269 T3915 T3912 nummod 0.15,μm
R2270 T3916 T3912 prep to,μm
R2271 T3917 T3918 nummod 2.29,μm
R2272 T3918 T3916 pobj μm,to
R2273 T3919 T3912 punct ),μm
R2274 T3920 T3877 punct .,were
R2275 T3922 T3923 amod Mitochondrial,width
R2276 T3923 T3924 nsubjpass width,altered
R2277 T3925 T3924 auxpass was,altered
R2278 T3926 T3924 neg not,altered
R2279 T3927 T3924 prep in,altered
R2280 T3928 T3929 amod homozygous,cells
R2281 T3929 T3927 pobj cells,in
R2282 T3930 T3929 nmod CbCln3Δex7,cells
R2283 T3931 T3930 punct /,CbCln3Δex7
R2284 T3932 T3930 nummod 8,CbCln3Δex7
R2285 T3933 T3934 punct (,shown
R2286 T3934 T3924 parataxis shown,altered
R2287 T3935 T3934 nsubj data,shown
R2288 T3936 T3934 neg not,shown
R2289 T3937 T3934 punct ),shown
R2290 T3938 T3924 punct .,altered
R2291 T3940 T3941 advmod Moreover,had
R2292 T3942 T3941 punct ", ",had
R2293 T3943 T3941 prep compared,had
R2294 T3944 T3943 prep to,compared
R2295 T3945 T3946 amod wild,type
R2296 T3946 T3948 nmod type,cells
R2297 T3947 T3946 punct -,type
R2298 T3948 T3944 pobj cells,to
R2299 T3949 T3946 cc or,type
R2300 T3950 T3946 conj heterozygous,type
R2301 T3951 T3941 punct ", ",had
R2302 T3952 T3953 amod homozygous,cells
R2303 T3953 T3941 nsubj cells,had
R2304 T3954 T3953 nmod CbCln3Δex7,cells
R2305 T3955 T3954 punct /,CbCln3Δex7
R2306 T3956 T3954 nummod 8,CbCln3Δex7
R2307 T3957 T3958 advmod significantly,reduced
R2308 T3958 T3959 amod reduced,levels
R2309 T3959 T3941 dobj levels,had
R2310 T3960 T3961 amod cellular,ATP
R2311 T3961 T3959 compound ATP,levels
R2312 T3962 T3963 punct (,Fig.
R2313 T3963 T3941 parataxis Fig.,had
R2314 T3964 T3965 npadvmod 1.3,less
R2315 T3965 T3963 amod less,Fig.
R2316 T3966 T3964 advmod fold,1.3
R2317 T3967 T3963 punct ", ",Fig.
R2318 T3968 T3963 nummod 8b,Fig.
R2319 T3969 T3963 punct ),Fig.
R2320 T3970 T3941 cc and,had
R2321 T3971 T3941 conj exhibited,had
R2322 T3972 T3973 amod reduced,survival
R2323 T3973 T3971 dobj survival,exhibited
R2324 T3974 T3971 prep following,exhibited
R2325 T3975 T3976 compound hydrogen,peroxide
R2326 T3976 T3977 compound peroxide,treatment
R2327 T3977 T3974 pobj treatment,following
R2328 T3978 T3979 punct (,8c
R2329 T3979 T3971 parataxis 8c,exhibited
R2330 T3980 T3981 punct ~,50
R2331 T3981 T3982 nummod 50,%
R2332 T3982 T3979 dep %,8c
R2333 T3983 T3982 prep of,%
R2334 T3984 T3985 amod wild,type
R2335 T3985 T3987 compound type,survival
R2336 T3986 T3985 punct -,type
R2337 T3987 T3983 pobj survival,of
R2338 T3988 T3979 punct ", ",8c
R2339 T3989 T3979 compound Fig.,8c
R2340 T3990 T3979 punct ),8c
R2341 T3991 T3941 punct ", ",had
R2342 T3992 T3941 advcl suggesting,had
R2343 T3993 T3994 amod impaired,metabolism
R2344 T3994 T3992 dobj metabolism,suggesting
R2345 T3995 T3994 compound energy,metabolism
R2346 T3996 T3994 cc and,metabolism
R2347 T3997 T3998 amod oxidative,response
R2348 T3998 T3994 conj response,metabolism
R2349 T3999 T3998 compound stress,response
R2350 T4000 T3941 punct .,had
R2351 T4002 T4003 advcl Taken,support
R2352 T4004 T4002 advmod together,Taken
R2353 T4005 T4003 punct ", ",support
R2354 T4006 T4007 det these,data
R2355 T4007 T4003 nsubj data,support
R2356 T4008 T4009 amod impaired,function
R2357 T4009 T4003 dobj function,support
R2358 T4010 T4009 amod mitochondrial,function
R2359 T4011 T4009 prep in,function
R2360 T4012 T4013 amod homozygous,cells
R2361 T4013 T4011 pobj cells,in
R2362 T4014 T4013 nmod CbCln3Δex7,cells
R2363 T4015 T4014 punct /,CbCln3Δex7
R2364 T4016 T4014 nummod 8,CbCln3Δex7
R2365 T4017 T4003 punct .,support
R2382 T4415 T4416 nmod CbCln3Δex7,cells
R2383 T4416 T4421 nsubj cells,represent
R2384 T4417 T4415 punct /,CbCln3Δex7
R2385 T4418 T4415 nummod 8,CbCln3Δex7
R2386 T4419 T4420 amod cerebellar,precursor
R2387 T4420 T4416 compound precursor,cells
R2388 T4422 T4423 det the,model
R2389 T4423 T4421 dobj model,represent
R2390 T4424 T4423 amod first,model
R2391 T4425 T4426 advmod genetically,accurate
R2392 T4426 T4423 amod accurate,model
R2393 T4427 T4428 npadvmod neuron,derived
R2394 T4428 T4423 amod derived,model
R2395 T4429 T4428 punct -,derived
R2396 T4430 T4423 compound culture,model
R2397 T4431 T4423 prep of,model
R2398 T4432 T4431 pobj JNCL,of
R2399 T4433 T4421 punct .,represent
R2400 T4435 T4436 amod Homozygous,cells
R2401 T4436 T4440 nsubj cells,express
R2402 T4437 T4436 nmod CbCln3Δex7,cells
R2403 T4438 T4437 punct /,CbCln3Δex7
R2404 T4439 T4437 nummod 8,CbCln3Δex7
R2405 T4441 T4442 compound mutant,battenin
R2406 T4442 T4440 dobj battenin,express
R2407 T4443 T4442 cc and,battenin
R2408 T4444 T4445 nmod JNCL,hallmark
R2409 T4445 T4447 nmod hallmark,accumulation
R2410 T4446 T4445 punct -,hallmark
R2411 T4447 T4442 conj accumulation,battenin
R2412 T4448 T4447 amod mitochondrial,accumulation
R2413 T4449 T4447 compound ATPase,accumulation
R2414 T4450 T4451 compound subunit,c
R2415 T4451 T4447 compound c,accumulation
R2416 T4452 T4440 punct ", ",express
R2417 T4453 T4440 prep upon,express
R2418 T4454 T4453 pobj aging,upon
R2419 T4455 T4454 prep of,aging
R2420 T4456 T4455 pobj cells,of
R2421 T4457 T4456 prep at,cells
R2422 T4458 T4457 pobj confluency,at
R2423 T4459 T4440 punct .,express
R2424 T4461 T4462 advmod Moreover,is
R2425 T4463 T4462 punct ", ",is
R2426 T4464 T4462 nsubj this,is
R2427 T4465 T4466 det the,study
R2428 T4466 T4462 attr study,is
R2429 T4467 T4466 amod first,study
R2430 T4468 T4469 aux to,indicate
R2431 T4469 T4466 advcl indicate,study
R2432 T4470 T4471 amod recessive,defects
R2433 T4471 T4469 dobj defects,indicate
R2434 T4472 T4473 amod endosomal,lysosomal
R2435 T4473 T4475 amod lysosomal,membrane
R2436 T4474 T4473 punct /,lysosomal
R2437 T4475 T4471 compound membrane,defects
R2438 T4476 T4471 compound trafficking,defects
R2439 T4477 T4471 cc and,defects
R2440 T4478 T4479 amod mitochondrial,dysfunction
R2441 T4479 T4471 conj dysfunction,defects
R2442 T4480 T4481 dep that,precedes
R2443 T4481 T4471 relcl precedes,defects
R2444 T4482 T4483 compound subunit,c
R2445 T4483 T4484 compound c,deposition
R2446 T4484 T4481 dobj deposition,precedes
R2447 T4485 T4481 prep in,precedes
R2448 T4486 T4487 det an,model
R2449 T4487 T4485 pobj model,in
R2450 T4488 T4487 amod accurate,model
R2451 T4489 T4487 compound JNCL,model
R2452 T4490 T4462 punct .,is
R2453 T4492 T4493 advmod Importantly,are
R2454 T4494 T4493 punct ", ",are
R2455 T4495 T4496 det these,defects
R2456 T4496 T4493 nsubj defects,are
R2457 T4497 T4493 acomp likely,are
R2458 T4498 T4499 aux to,be
R2459 T4499 T4497 xcomp be,likely
R2460 T4500 T4501 amod early,events
R2461 T4501 T4499 attr events,be
R2462 T4502 T4501 prep in,events
R2463 T4503 T4504 det the,process
R2464 T4504 T4502 pobj process,in
R2465 T4505 T4504 compound JNCL,process
R2466 T4506 T4504 compound disease,process
R2467 T4507 T4493 cc and,are
R2468 T4508 T4509 aux may,impact
R2469 T4509 T4493 conj impact,are
R2470 T4510 T4509 advmod particularly,impact
R2471 T4511 T4512 amod neuronal,function
R2472 T4512 T4509 dobj function,impact
R2473 T4513 T4493 punct .,are
R2474 T4515 T4516 amod Abnormal,D
R2475 T4516 T4518 nsubj D,reflects
R2476 T4517 T4516 compound cathepsin,D
R2477 T4519 T4516 appos localization,D
R2478 T4520 T4519 cc and,localization
R2479 T4521 T4519 conj processing,localization
R2480 T4522 T4516 prep in,D
R2481 T4523 T4524 amod homozygous,cells
R2482 T4524 T4522 pobj cells,in
R2483 T4525 T4524 nmod CbCln3Δex7,cells
R2484 T4526 T4525 punct /,CbCln3Δex7
R2485 T4527 T4525 nummod 8,CbCln3Δex7
R2486 T4528 T4524 cc and,cells
R2487 T4529 T4530 nmod Cln3Δex7,mice
R2488 T4530 T4524 conj mice,cells
R2489 T4531 T4529 punct /,Cln3Δex7
R2490 T4532 T4529 nummod 8,Cln3Δex7
R2491 T4533 T4518 advmod likely,reflects
R2492 T4534 T4535 amod altered,trafficking
R2493 T4535 T4518 dobj trafficking,reflects
R2494 T4536 T4535 amod vesicular,trafficking
R2495 T4537 T4535 cc and,trafficking
R2496 T4538 T4535 punct /,trafficking
R2497 T4539 T4535 cc or,trafficking
R2498 T4540 T4541 amod lysosomal,pH
R2499 T4541 T4535 conj pH,trafficking
R2500 T4542 T4535 punct ", ",trafficking
R2501 T4543 T4544 dep which,known
R2502 T4544 T4535 relcl known,trafficking
R2503 T4545 T4544 auxpass is,known
R2504 T4546 T4547 aux to,impact
R2505 T4547 T4544 xcomp impact,known
R2506 T4548 T4549 compound cathepsin,D
R2507 T4549 T4550 compound D,processing
R2508 T4550 T4547 dobj processing,impact
R2509 T4551 T4552 punct [,16
R2510 T4552 T4544 parataxis 16,known
R2511 T4553 T4552 nummod 14,16
R2512 T4554 T4552 punct ",",16
R2513 T4555 T4552 punct ],16
R2514 T4556 T4518 punct .,reflects
R2515 T4558 T4559 advmod Indeed,altered
R2516 T4560 T4559 punct ", ",altered
R2517 T4561 T4562 compound CLN3,overexpression
R2518 T4562 T4559 nsubj overexpression,altered
R2519 T4563 T4562 prep in,overexpression
R2520 T4564 T4565 nmod HEK,cells
R2521 T4565 T4563 pobj cells,in
R2522 T4566 T4564 punct -,HEK
R2523 T4567 T4564 nummod 293,HEK
R2524 T4568 T4569 amod lysosomal,pH
R2525 T4569 T4570 nmod pH,processing
R2526 T4570 T4559 dobj processing,altered
R2527 T4571 T4569 cc and,pH
R2528 T4572 T4573 compound cathepsin,D
R2529 T4573 T4569 conj D,pH
R2530 T4574 T4575 punct [,17
R2531 T4575 T4559 parataxis 17,altered
R2532 T4576 T4575 punct ],17
R2533 T4577 T4559 punct ", ",altered
R2534 T4578 T4559 cc and,altered
R2535 T4579 T4580 amod lysosomal,pH
R2536 T4580 T4581 compound pH,homeostasis
R2537 T4581 T4582 nsubjpass homeostasis,disrupted
R2538 T4582 T4559 conj disrupted,altered
R2539 T4583 T4582 auxpass is,disrupted
R2540 T4584 T4582 prep in,disrupted
R2541 T4585 T4584 pobj JNCL,in
R2542 T4586 T4587 punct [,15
R2543 T4587 T4582 parataxis 15,disrupted
R2544 T4588 T4587 nummod 10,15
R2545 T4589 T4587 punct ",",15
R2546 T4590 T4587 punct ],15
R2547 T4591 T4582 punct .,disrupted
R2548 T4593 T4594 nsubj It,is
R2549 T4595 T4594 acomp noteworthy,is
R2550 T4596 T4597 mark that,altered
R2551 T4597 T4594 ccomp altered,is
R2552 T4598 T4599 compound cathepsin,B
R2553 T4599 T4597 nsubjpass B,altered
R2554 T4600 T4599 cc and,B
R2555 T4601 T4602 det the,enzyme
R2556 T4602 T4599 conj enzyme,B
R2557 T4603 T4604 npadvmod CLN2,encoded
R2558 T4604 T4602 amod encoded,enzyme
R2559 T4605 T4604 punct -,encoded
R2560 T4606 T4602 punct ", ",enzyme
R2561 T4607 T4602 appos TPPI,enzyme
R2562 T4608 T4597 punct ", ",altered
R2563 T4609 T4597 auxpass are,altered
R2564 T4610 T4597 advmod also,altered
R2565 T4611 T4597 prep in,altered
R2566 T4612 T4611 pobj JNCL,in
R2567 T4613 T4614 punct [,18
R2568 T4614 T4597 parataxis 18,altered
R2569 T4615 T4616 punct -,20
R2570 T4616 T4614 prep 20,18
R2571 T4617 T4614 punct ],18
R2572 T4618 T4594 punct .,is
R2573 T4620 T4621 advmod Nevertheless,appear
R2574 T4622 T4621 punct ", ",appear
R2575 T4623 T4621 prep despite,appear
R2576 T4624 T4625 det the,alterations
R2577 T4625 T4623 pobj alterations,despite
R2578 T4626 T4627 compound cathepsin,D
R2579 T4627 T4625 compound D,alterations
R2580 T4628 T4625 compound protein,alterations
R2581 T4629 T4630 dep that,observed
R2582 T4630 T4625 relcl observed,alterations
R2583 T4631 T4630 auxpass are,observed
R2584 T4632 T4630 prep in,observed
R2585 T4633 T4634 amod homozygous,cells
R2586 T4634 T4632 pobj cells,in
R2587 T4635 T4634 nmod CbCln3Δex7,cells
R2588 T4636 T4635 punct /,CbCln3Δex7
R2589 T4637 T4635 nummod 8,CbCln3Δex7
R2590 T4638 T4621 punct ", ",appear
R2591 T4639 T4640 nmod cathepsin,D
R2592 T4640 T4641 nmod D,activity
R2593 T4641 T4621 nsubj activity,appear
R2594 T4642 T4641 amod enzymatic,activity
R2595 T4643 T4621 aux does,appear
R2596 T4644 T4621 neg not,appear
R2597 T4645 T4646 aux to,reduced
R2598 T4646 T4621 xcomp reduced,appear
R2599 T4647 T4646 auxpass be,reduced
R2600 T4648 T4621 punct .,appear
R2601 T4650 T4651 advmod Thus,is
R2602 T4652 T4651 punct ", ",is
R2603 T4653 T4654 amod decreased,activity
R2604 T4654 T4651 nsubj activity,is
R2605 T4655 T4656 compound cathepsin,D
R2606 T4656 T4654 compound D,activity
R2607 T4657 T4651 acomp unlikely,is
R2608 T4658 T4659 aux to,account
R2609 T4659 T4657 xcomp account,unlikely
R2610 T4660 T4659 prep for,account
R2611 T4661 T4662 compound subunit,c
R2612 T4662 T4663 compound c,accumulation
R2613 T4663 T4660 pobj accumulation,for
R2614 T4664 T4659 prep in,account
R2615 T4665 T4664 pobj JNCL,in
R2616 T4666 T4651 punct .,is
R2617 T4668 T4669 nsubj Aging,is
R2618 T4670 T4668 prep of,Aging
R2619 T4671 T4672 amod homozygous,cells
R2620 T4672 T4670 pobj cells,of
R2621 T4673 T4672 nmod CbCln3Δex7,cells
R2622 T4674 T4673 punct /,CbCln3Δex7
R2623 T4675 T4673 nummod 8,CbCln3Δex7
R2624 T4676 T4668 prep at,Aging
R2625 T4677 T4676 pobj confluency,at
R2626 T4678 T4669 acomp necessary,is
R2627 T4679 T4680 aux to,induce
R2628 T4680 T4678 xcomp induce,necessary
R2629 T4681 T4682 advmod significantly,accumulated
R2630 T4682 T4683 amod accumulated,protein
R2631 T4683 T4680 dobj protein,induce
R2632 T4684 T4685 compound subunit,c
R2633 T4685 T4683 compound c,protein
R2634 T4686 T4669 punct .,is
R2635 T4688 T4689 advmod However,precede
R2636 T4690 T4689 punct ", ",precede
R2637 T4691 T4692 compound membrane,organelle
R2638 T4692 T4693 compound organelle,disruptions
R2639 T4693 T4689 nsubj disruptions,precede
R2640 T4694 T4695 compound subunit,c
R2641 T4695 T4696 compound c,accumulation
R2642 T4696 T4689 dobj accumulation,precede
R2643 T4697 T4689 prep in,precede
R2644 T4698 T4699 amod homozygous,cells
R2645 T4699 T4697 pobj cells,in
R2646 T4700 T4699 nmod CbCln3Δex7,cells
R2647 T4701 T4700 punct /,CbCln3Δex7
R2648 T4702 T4700 nummod 8,CbCln3Δex7
R2649 T4703 T4689 punct ", ",precede
R2650 T4704 T4705 mark since,observed
R2651 T4705 T4689 advcl observed,precede
R2652 T4706 T4705 nsubjpass they,observed
R2653 T4707 T4705 auxpass are,observed
R2654 T4708 T4705 prep without,observed
R2655 T4709 T4708 pobj aging,without
R2656 T4710 T4705 prep at,observed
R2657 T4711 T4710 pobj confluency,at
R2658 T4712 T4689 punct .,precede
R2659 T4714 T4715 amod Lysosomal,size
R2660 T4715 T4718 nsubjpass size,altered
R2661 T4716 T4714 cc and,Lysosomal
R2662 T4717 T4714 conj endosomal,Lysosomal
R2663 T4719 T4715 cc and,size
R2664 T4720 T4715 conj distribution,size
R2665 T4721 T4718 auxpass are,altered
R2666 T4722 T4718 punct ", ",altered
R2667 T4723 T4718 cc and,altered
R2668 T4724 T4725 nsubj mitochondria,are
R2669 T4725 T4718 conj are,altered
R2670 T4726 T4727 advmod abnormally,elongated
R2671 T4727 T4725 acomp elongated,are
R2672 T4728 T4727 cc and,elongated
R2673 T4729 T4730 advmod functionally,compromised
R2674 T4730 T4727 conj compromised,elongated
R2675 T4731 T4725 prep in,are
R2676 T4732 T4733 amod sub-confluent,cultures
R2677 T4733 T4731 pobj cultures,in
R2678 T4734 T4733 amod homozygous,cultures
R2679 T4735 T4733 nmod CbCln3Δex7,cultures
R2680 T4736 T4735 punct /,CbCln3Δex7
R2681 T4737 T4735 nummod 8,CbCln3Δex7
R2682 T4738 T4725 punct .,are
R2683 T4740 T4741 det These,observations
R2684 T4741 T4742 nsubj observations,argue
R2685 T4743 T4744 mark that,result
R2686 T4744 T4742 ccomp result,argue
R2687 T4745 T4746 compound membrane,trafficking
R2688 T4746 T4747 compound trafficking,defects
R2689 T4747 T4744 nsubj defects,result
R2690 T4748 T4744 aux do,result
R2691 T4749 T4744 neg not,result
R2692 T4750 T4744 prep from,result
R2693 T4751 T4752 amod excessive,accumulation
R2694 T4752 T4755 nsubj accumulation,compromising
R2695 T4753 T4754 compound subunit,c
R2696 T4754 T4752 compound c,accumulation
R2697 T4755 T4750 pcomp compromising,from
R2698 T4756 T4757 det the,lysosome
R2699 T4757 T4755 dobj lysosome,compromising
R2700 T4758 T4744 punct ", ",result
R2701 T4759 T4744 cc but,result
R2702 T4760 T4759 advmod rather,but
R2703 T4761 T4744 conj are,result
R2704 T4762 T4763 amod early,events
R2705 T4763 T4761 attr events,are
R2706 T4764 T4763 prep in,events
R2707 T4765 T4766 det the,process
R2708 T4766 T4764 pobj process,in
R2709 T4767 T4766 compound disease,process
R2710 T4768 T4763 acl preceding,events
R2711 T4769 T4770 compound subunit,c
R2712 T4770 T4771 compound c,accumulation
R2713 T4771 T4768 dobj accumulation,preceding
R2714 T4772 T4742 punct .,argue
R2715 T4774 T4775 amod Mitochondrial,abnormalities
R2716 T4775 T4776 nsubj abnormalities,result
R2717 T4777 T4775 punct ", ",abnormalities
R2718 T4778 T4779 dep which,reported
R2719 T4779 T4775 relcl reported,abnormalities
R2720 T4780 T4779 aux have,reported
R2721 T4781 T4779 advmod also,reported
R2722 T4782 T4779 auxpass been,reported
R2723 T4783 T4779 prep in,reported
R2724 T4784 T4785 compound JNCL,patients
R2725 T4785 T4783 pobj patients,in
R2726 T4786 T4785 cc and,patients
R2727 T4787 T4788 amod other,models
R2728 T4788 T4785 conj models,patients
R2729 T4789 T4788 compound animal,models
R2730 T4790 T4791 punct [,21
R2731 T4791 T4779 parataxis 21,reported
R2732 T4792 T4793 punct -,23
R2733 T4793 T4791 prep 23,21
R2734 T4794 T4791 punct ],21
R2735 T4795 T4776 punct ", ",result
R2736 T4796 T4776 aux may,result
R2737 T4797 T4776 prep from,result
R2738 T4798 T4799 amod ineffective,turnover
R2739 T4799 T4797 pobj turnover,from
R2740 T4800 T4799 prep by,turnover
R2741 T4801 T4800 pobj autophagy,by
R2742 T4802 T4801 punct ", ",autophagy
R2743 T4803 T4804 det a,pathway
R2744 T4804 T4801 appos pathway,autophagy
R2745 T4805 T4806 amod lysosomal,targeted
R2746 T4806 T4804 amod targeted,pathway
R2747 T4807 T4806 punct -,targeted
R2748 T4808 T4809 punct [,24
R2749 T4809 T4776 parataxis 24,result
R2750 T4810 T4809 punct ],24
R2751 T4811 T4776 punct .,result
R2752 T4813 T4814 advmod Alternatively,impact
R2753 T4815 T4813 punct ", ",Alternatively
R2754 T4816 T4813 cc or,Alternatively
R2755 T4817 T4813 conj simultaneously,Alternatively
R2756 T4818 T4814 punct ", ",impact
R2757 T4819 T4820 compound battenin,deficiency
R2758 T4820 T4814 nsubj deficiency,impact
R2759 T4821 T4814 aux may,impact
R2760 T4822 T4823 amod mitochondrial,function
R2761 T4823 T4814 dobj function,impact
R2762 T4824 T4823 advmod upstream,function
R2763 T4825 T4824 prep of,upstream
R2764 T4826 T4825 pobj turnover,of
R2765 T4827 T4814 punct ", ",impact
R2766 T4828 T4814 advcl affecting,impact
R2767 T4829 T4830 amod mitochondrial,biogenesis
R2768 T4830 T4828 dobj biogenesis,affecting
R2769 T4831 T4830 cc and,biogenesis
R2770 T4832 T4831 punct /,and
R2771 T4833 T4831 cc or,and
R2772 T4834 T4835 amod altered,transport
R2773 T4835 T4830 conj transport,biogenesis
R2774 T4836 T4835 cc and,transport
R2775 T4837 T4835 conj processing,transport
R2776 T4838 T4835 prep of,transport
R2777 T4839 T4840 amod mitochondrial,proteins
R2778 T4840 T4838 pobj proteins,of
R2779 T4841 T4814 punct .,impact
R2780 T4843 T4844 prep In,co-localizes
R2781 T4845 T4846 amod wild,type
R2782 T4846 T4848 nmod type,cells
R2783 T4847 T4846 punct -,type
R2784 T4848 T4843 pobj cells,In
R2785 T4849 T4848 nmod CbCln3,cells
R2786 T4850 T4851 amod neuronal,precursor
R2787 T4851 T4848 compound precursor,cells
R2788 T4852 T4844 nsubj battenin,co-localizes
R2789 T4853 T4844 advmod primarily,co-localizes
R2790 T4854 T4844 prep with,co-localizes
R2791 T4855 T4856 amod early,endosomes
R2792 T4856 T4854 pobj endosomes,with
R2793 T4857 T4855 cc and,early
R2794 T4858 T4855 conj late,early
R2795 T4859 T4844 punct .,co-localizes
R2796 T4861 T4862 compound Battenin,immunostaining
R2797 T4862 T4863 nsubjpass immunostaining,reduced
R2798 T4864 T4862 prep in,immunostaining
R2799 T4865 T4866 amod homozygous,precursors
R2800 T4866 T4864 pobj precursors,in
R2801 T4867 T4866 nmod CbCln3Δex7,precursors
R2802 T4868 T4867 punct /,CbCln3Δex7
R2803 T4869 T4867 nummod 8,CbCln3Δex7
R2804 T4870 T4866 amod neuronal,precursors
R2805 T4871 T4863 auxpass is,reduced
R2806 T4872 T4863 advmod significantly,reduced
R2807 T4873 T4863 prep in,reduced
R2808 T4874 T4873 pobj abundance,in
R2809 T4875 T4863 punct ", ",reduced
R2810 T4876 T4863 cc but,reduced
R2811 T4877 T4878 compound mutant,signal
R2812 T4878 T4879 nsubj signal,co-localizes
R2813 T4879 T4863 conj co-localizes,reduced
R2814 T4880 T4879 advmod also,co-localizes
R2815 T4881 T4879 prep with,co-localizes
R2816 T4882 T4883 amod endosomal,markers
R2817 T4883 T4881 pobj markers,with
R2818 T4884 T4879 advcl suggesting,co-localizes
R2819 T4885 T4886 compound mutant,protein
R2820 T4886 T4888 nsubjpass protein,trafficked
R2821 T4887 T4886 compound battenin,protein
R2822 T4888 T4884 advcl trafficked,suggesting
R2823 T4889 T4886 prep with,protein
R2824 T4890 T4891 npadvmod C,terminal
R2825 T4891 T4893 amod terminal,epitopes
R2826 T4892 T4891 punct -,terminal
R2827 T4893 T4889 pobj epitopes,with
R2828 T4894 T4888 auxpass is,trafficked
R2829 T4895 T4888 advcl similar,trafficked
R2830 T4896 T4895 prep to,similar
R2831 T4897 T4898 amod wild,type
R2832 T4898 T4900 compound type,protein
R2833 T4899 T4898 punct -,type
R2834 T4900 T4896 pobj protein,to
R2835 T4901 T4879 punct .,co-localizes
R2836 T4903 T4904 prep In,reported
R2837 T4905 T4906 amod other,studies
R2838 T4906 T4903 pobj studies,In
R2839 T4907 T4904 punct ", ",reported
R2840 T4908 T4909 nmod CLN3,localization
R2841 T4909 T4904 nsubjpass localization,reported
R2842 T4910 T4908 punct /,CLN3
R2843 T4911 T4912 compound battenin,protein
R2844 T4912 T4908 appos protein,CLN3
R2845 T4913 T4904 aux has,reported
R2846 T4914 T4904 auxpass been,reported
R2847 T4915 T4916 aux to,overlap
R2848 T4916 T4904 xcomp overlap,reported
R2849 T4917 T4916 advmod partially,overlap
R2850 T4918 T4916 prep with,overlap
R2858 T4926 T4927 punct [,7
R2859 T4927 T4916 parataxis 7,overlap
R2860 T4928 T4927 punct ],7
R2861 T4929 T4916 punct ", ",overlap
R2862 T4930 T4916 cc and,overlap
R2863 T4931 T4916 conj to,overlap
R2864 T4932 T4931 pobj lysosomes,to
R2865 T4933 T4932 punct ", ",lysosomes
R2866 T4934 T4932 conj synaptosomes,lysosomes
R2867 T4935 T4936 punct [,8
R2868 T4936 T4934 parataxis 8,synaptosomes
R2869 T4937 T4936 punct ],8
R2870 T4938 T4934 cc and,synaptosomes
R2871 T4939 T4934 conj endosomes,synaptosomes
R2872 T4940 T4941 punct [,25
R2873 T4941 T4939 parataxis 25,endosomes
R2874 T4942 T4941 nummod 9,25
R2875 T4943 T4941 punct ",",25
R2876 T4944 T4941 punct ],25
R2877 T4945 T4931 prep in,to
R2878 T4946 T4945 pobj neurons,in
R2879 T4947 T4904 punct .,reported
R2880 T4949 T4950 det These,data
R2881 T4950 T4951 nsubj data,indicate
R2882 T4952 T4951 advmod jointly,indicate
R2883 T4953 T4954 mark that,resides
R2884 T4954 T4951 ccomp resides,indicate
R2885 T4955 T4954 nsubj battenin,resides
R2886 T4956 T4954 prep in,resides
R2887 T4957 T4958 det a,subset
R2888 T4958 T4956 pobj subset,in
R2889 T4959 T4958 prep of,subset
R2890 T4960 T4961 amod vesicular,compartments
R2891 T4961 T4959 pobj compartments,of
R2892 T4962 T4961 acl linking,compartments
R2893 T4963 T4964 amod multiple,pathways
R2894 T4964 T4962 dobj pathways,linking
R2895 T4965 T4966 compound membrane,trafficking
R2896 T4966 T4964 compound trafficking,pathways
R2897 T4967 T4954 punct ", ",resides
R2898 T4968 T4969 advmod perhaps,functioning
R2899 T4969 T4954 advcl functioning,resides
R2900 T4970 T4969 prep in,functioning
R2901 T4971 T4972 amod vesicular,transport
R2902 T4972 T4970 pobj transport,in
R2903 T4973 T4972 cc and,transport
R2904 T4974 T4973 punct /,and
R2905 T4975 T4973 cc or,and
R2906 T4976 T4972 conj fusion,transport
R2907 T4977 T4951 punct .,indicate
R2908 T4979 T4980 amod Endocytic,targeted
R2909 T4980 T4984 amod targeted,pathways
R2910 T4981 T4979 cc and,Endocytic
R2911 T4982 T4979 conj lysosomal,Endocytic
R2912 T4983 T4980 punct -,targeted
R2913 T4984 T4985 nsubjpass pathways,implicated
R2914 T4986 T4984 punct ", ",pathways
R2915 T4987 T4984 prep including,pathways
R2916 T4988 T4989 amod mitochondrial,autophagy
R2917 T4989 T4987 pobj autophagy,including
R2918 T4990 T4985 punct ", ",implicated
R2919 T4991 T4985 auxpass are,implicated
R2920 T4992 T4985 advmod specifically,implicated
R2921 T4993 T4985 prep in,implicated
R2922 T4994 T4995 det this,study
R2923 T4995 T4993 pobj study,in
R2924 T4996 T4985 punct .,implicated
R2953 T5078 T5079 det a,metabolism
R2954 T5079 T5083 nsubj metabolism,sensitize
R2955 T5080 T5081 amod high,energy
R2956 T5081 T5079 compound energy,metabolism
R2957 T5082 T5081 punct -,energy
R2958 T5083 T5070 conj sensitize,relies
R2959 T5084 T5083 aux may,sensitize
R2960 T5085 T5083 advmod further,sensitize
R2961 T5086 T5083 dobj neurons,sensitize
R2962 T5087 T5083 prep to,sensitize
R2963 T5088 T5089 det the,loss
R2964 T5089 T5087 pobj loss,to
R2965 T5090 T5089 prep of,loss
R2966 T5091 T5092 compound battenin,activity
R2967 T5092 T5090 pobj activity,of
R2968 T5093 T5083 punct .,sensitize
R2969 T5095 T5096 advmod Thus,provide
R2970 T5097 T5096 punct ", ",provide
R2971 T5098 T5099 poss our,panel
R2972 T5099 T5096 nsubj panel,provide
R2973 T5100 T5099 prep of,panel
R2974 T5101 T5102 amod wild,type
R2975 T5102 T5104 nmod type,cells
R2976 T5103 T5102 punct -,type
R2977 T5104 T5100 pobj cells,of
R2978 T5105 T5102 punct ", ",type
R2979 T5106 T5102 conj heterozygous,type
R2980 T5107 T5106 punct ", ",heterozygous
R2981 T5108 T5106 cc and,heterozygous
R2982 T5109 T5106 conj homozygous,heterozygous
R2983 T5110 T5104 nmod CbCln3Δex7,cells
R2984 T5111 T5110 punct /,CbCln3Δex7
R2985 T5112 T5110 nummod 8,CbCln3Δex7
R2986 T5113 T5104 amod cerebellar,cells
R2987 T5114 T5115 det an,system
R2988 T5115 T5096 dobj system,provide
R2989 T5116 T5115 amod ideal,system
R2990 T5117 T5115 compound model,system
R2991 T5118 T5119 aux to,elucidate
R2992 T5119 T5115 advcl elucidate,system
R2993 T5120 T5119 advmod further,elucidate
R2994 T5121 T5122 compound battenin,function
R2995 T5122 T5119 dobj function,elucidate
R2996 T5123 T5122 cc and,function
R2997 T5124 T5125 compound JNCL,pathogenesis
R2998 T5125 T5122 conj pathogenesis,function
R2999 T5126 T5096 punct .,provide
R3000 T5236 T5237 nmod Antibody,reagents
R3001 T5238 T5236 cc and,Antibody
R3002 T5239 T5240 compound cell,staining
R3003 T5240 T5236 conj staining,Antibody
R3004 T5242 T5243 nmod Nestin,antibodies
R3005 T5243 T5282 nsubjpass antibodies,obtained
R3006 T5244 T5245 punct (,μg
R3007 T5245 T5242 parataxis μg,Nestin
R3008 T5246 T5247 compound clone,Rat
R3009 T5247 T5245 dep Rat,μg
R3010 T5248 T5247 nummod 401,Rat
R3011 T5249 T5245 punct ", ",μg
R3012 T5250 T5245 nummod 2,μg
R3013 T5251 T5252 punct /,ml
R3014 T5252 T5245 prep ml,μg
R3015 T5253 T5245 punct ),μg
R3016 T5254 T5242 punct ", ",Nestin
R3017 T5255 T5242 conj Lamp,Nestin
R3018 T5256 T5255 nummod 1,Lamp
R3019 T5257 T5258 punct (,μg
R3020 T5258 T5255 parataxis μg,Lamp
R3021 T5259 T5260 compound clone,1D4B
R3022 T5260 T5258 dep 1D4B,μg
R3023 T5261 T5258 punct ", ",μg
R3024 T5262 T5258 nummod 6,μg
R3025 T5263 T5264 punct /,ml
R3026 T5264 T5258 prep ml,μg
R3027 T5265 T5258 punct ),μg
R3028 T5266 T5255 punct ", ",Lamp
R3029 T5267 T5255 cc and,Lamp
R3030 T5268 T5255 conj Lamp,Lamp
R3031 T5269 T5268 nummod 2,Lamp
R3032 T5270 T5271 punct (,μg
R3033 T5271 T5268 parataxis μg,Lamp
R3034 T5272 T5273 compound clone,Abl
R3035 T5273 T5271 dep Abl,μg
R3036 T5274 T5273 punct -,Abl
R3037 T5275 T5273 nummod 93,Abl
R3038 T5276 T5271 punct ;,μg
R3039 T5277 T5271 nummod 6,μg
R3040 T5278 T5279 punct /,ml
R3041 T5279 T5271 prep ml,μg
R3042 T5280 T5271 punct ),μg
R3043 T5281 T5243 amod monoclonal,antibodies
R3044 T5283 T5282 auxpass were,obtained
R3045 T5284 T5282 prep from,obtained
R3046 T5285 T5286 det the,Bank
R3047 T5286 T5284 pobj Bank,from
R3048 T5287 T5286 compound Developmental,Bank
R3049 T5288 T5286 compound Studies,Bank
R3050 T5289 T5286 compound Hybridoma,Bank
R3051 T5290 T5286 punct ", ",Bank
R3052 T5291 T5286 acl maintained,Bank
R3053 T5292 T5291 agent by,maintained
R3054 T5293 T5294 det The,University
R3055 T5294 T5295 nmod University,Department
R3056 T5295 T5292 pobj Department,by
R3057 T5296 T5294 prep of,University
R3058 T5297 T5296 pobj Iowa,of
R3059 T5298 T5295 punct ", ",Department
R3060 T5299 T5295 prep of,Department
R3061 T5300 T5301 compound Biological,Sciences
R3062 T5301 T5299 pobj Sciences,of
R3063 T5302 T5282 punct .,obtained
R3064 T5304 T5305 nsubjpass Batp1,described
R3065 T5306 T5307 punct (,μg
R3066 T5307 T5304 parataxis μg,Batp1
R3067 T5308 T5307 nummod 1,μg
R3068 T5309 T5310 punct /,ml
R3069 T5310 T5307 prep ml,μg
R3070 T5311 T5307 punct ),μg
R3071 T5312 T5305 auxpass was,described
R3072 T5313 T5305 advmod previously,described
R3073 T5314 T5315 punct [,12
R3074 T5315 T5305 parataxis 12,described
R3075 T5316 T5315 punct ],12
R3076 T5317 T5305 punct .,described
R3077 T5319 T5320 amod Anti-subunit,c
R3078 T5320 T5321 amod c,antibody
R3079 T5321 T5322 nsubjpass antibody,provided
R3080 T5323 T5324 punct (,μg
R3081 T5324 T5321 parataxis μg,antibody
R3082 T5325 T5324 nummod 0.7,μg
R3083 T5326 T5327 punct /,ml
R3084 T5327 T5324 prep ml,μg
R3085 T5328 T5324 punct ),μg
R3086 T5329 T5322 auxpass was,provided
R3087 T5330 T5322 advmod kindly,provided
R3088 T5331 T5322 agent by,provided
R3089 T5332 T5333 compound Dr.,Kominami
R3090 T5333 T5331 pobj Kominami,by
R3091 T5334 T5335 punct (,University
R3092 T5335 T5333 parataxis University,Kominami
R3093 T5336 T5335 compound Juntendo,University
R3094 T5337 T5335 punct ", ",University
R3095 T5338 T5335 npadvmod Tokyo,University
R3096 T5339 T5335 punct ", ",University
R3097 T5340 T5335 npadvmod Japan,University
R3098 T5341 T5335 punct ),University
R3099 T5342 T5322 punct .,provided
R3100 T5344 T5345 amod Additional,antibodies
R3101 T5345 T5346 nsubj antibodies,were
R3102 T5347 T5348 mark as,follows
R3103 T5348 T5346 advcl follows,were
R3104 T5349 T5346 punct : ,were
R3105 T5350 T5346 dep GFAP,were
R3106 T5351 T5350 punct ", ",GFAP
R3107 T5352 T5350 npadvmod 1,GFAP
R3108 T5353 T5354 punct :,2000
R3109 T5354 T5352 prep 2000,1
R3110 T5355 T5356 punct (,Corporation
R3111 T5356 T5350 parataxis Corporation,GFAP
R3112 T5357 T5356 compound DAKO,Corporation
R3113 T5358 T5356 punct ),Corporation
R3114 T5359 T5350 punct ;,GFAP
R3115 T5360 T5350 appos calbindin,GFAP
R3116 T5361 T5360 punct ", ",calbindin
R3117 T5362 T5360 npadvmod 1,calbindin
R3118 T5363 T5364 punct :,5000
R3119 T5364 T5362 prep 5000,1
R3120 T5365 T5366 punct (,Sigma
R3121 T5366 T5360 parataxis Sigma,calbindin
R3122 T5367 T5366 punct ),Sigma
R3123 T5368 T5350 punct ;,GFAP
R3124 T5369 T5350 appos NeuN,GFAP
R3125 T5370 T5369 punct ", ",NeuN
R3126 T5371 T5372 nummod 10,μg
R3127 T5372 T5369 npadvmod μg,NeuN
R3128 T5373 T5374 punct /,ml
R3129 T5374 T5372 prep ml,μg
R3130 T5375 T5376 punct (,Chemicon
R3131 T5376 T5369 parataxis Chemicon,NeuN
R3132 T5377 T5376 punct ),Chemicon
R3133 T5378 T5350 punct ;,GFAP
R3134 T5379 T5380 compound SV40,antigen
R3135 T5380 T5350 appos antigen,GFAP
R3136 T5381 T5380 compound T,antigen
R3137 T5382 T5380 punct (,antigen
R3138 T5383 T5380 appos Pab,antigen
R3139 T5384 T5383 nummod 101,Pab
R3140 T5385 T5380 punct ),antigen
R3141 T5386 T5380 punct ", ",antigen
R3142 T5387 T5388 nummod 2,μg
R3143 T5388 T5380 npadvmod μg,antigen
R3144 T5389 T5390 punct /,ml
R3145 T5390 T5388 prep ml,μg
R3146 T5391 T5392 punct (,Biotechnology
R3147 T5392 T5380 parataxis Biotechnology,antigen
R3148 T5393 T5392 compound Santa,Biotechnology
R3149 T5394 T5392 compound Cruz,Biotechnology
R3150 T5395 T5392 punct ),Biotechnology
R3151 T5396 T5350 punct ;,GFAP
R3152 T5397 T5398 compound cathepsin,D
R3153 T5398 T5350 appos D,GFAP
R3154 T5399 T5398 punct (,D
R3155 T5400 T5398 appos C,D
R3156 T5401 T5400 punct -,C
R3157 T5402 T5400 nummod 20,C
R3158 T5403 T5398 punct ),D
R3159 T5404 T5398 punct ", ",D
R3160 T5405 T5406 nummod 2,μg
R3161 T5406 T5398 npadvmod μg,D
R3162 T5407 T5408 punct /,ml
R3163 T5408 T5406 prep ml,μg
R3164 T5409 T5410 punct (,Biotechnology
R3165 T5410 T5398 parataxis Biotechnology,D
R3166 T5411 T5410 compound Santa,Biotechnology
R3167 T5412 T5410 compound Cruz,Biotechnology
R3168 T5413 T5410 punct ),Biotechnology
R3169 T5414 T5350 punct ;,GFAP
R3170 T5415 T5416 compound cytochrome,subunit
R3171 T5416 T5350 appos subunit,GFAP
R3172 T5417 T5416 compound c,subunit
R3173 T5418 T5416 compound oxidase,subunit
R3174 T5419 T5416 nummod IV,subunit
R3175 T5420 T5416 punct (,subunit
R3176 T5421 T5416 appos cox4,subunit
R3177 T5422 T5416 punct ),subunit
R3178 T5423 T5416 punct ", ",subunit
R3179 T5424 T5416 npadvmod 1,subunit
R3180 T5425 T5426 punct :,1000
R3181 T5426 T5424 prep 1000,1
R3182 T5427 T5428 punct (,Clontech
R3183 T5428 T5416 parataxis Clontech,subunit
R3184 T5429 T5428 compound BD,Clontech
R3185 T5430 T5428 compound Biosciences,Clontech
R3186 T5431 T5428 punct ),Clontech
R3187 T5432 T5350 punct ;,GFAP
R3188 T5433 T5350 appos PDI,GFAP
R3189 T5434 T5433 punct (,PDI
R3190 T5435 T5433 appos H,PDI
R3191 T5436 T5435 punct -,H
R3192 T5437 T5435 nummod 160,H
R3193 T5438 T5433 punct ),PDI
R3194 T5439 T5433 punct ", ",PDI
R3195 T5440 T5441 nummod 2,μg
R3196 T5441 T5433 npadvmod μg,PDI
R3197 T5442 T5443 punct /,ml
R3198 T5443 T5441 prep ml,μg
R3199 T5444 T5445 punct (,Biotechnology
R3200 T5445 T5433 parataxis Biotechnology,PDI
R3201 T5446 T5445 compound Santa,Biotechnology
R3202 T5447 T5445 compound Cruz,Biotechnology
R3203 T5448 T5445 punct ),Biotechnology
R3204 T5449 T5350 punct ;,GFAP
R3205 T5450 T5350 appos GM130,GFAP
R3206 T5451 T5450 punct ", ",GM130
R3207 T5452 T5453 nummod 1,μg
R3208 T5453 T5450 npadvmod μg,GM130
R3209 T5454 T5455 punct /,ml
R3210 T5455 T5453 prep ml,μg
R3211 T5456 T5457 punct (,Labs
R3212 T5457 T5450 parataxis Labs,GM130
R3213 T5458 T5457 compound BD,Labs
R3214 T5459 T5457 compound Transduction,Labs
R3215 T5460 T5457 punct ),Labs
R3216 T5461 T5350 punct ;,GFAP
R3217 T5462 T5463 compound α,tubulin
R3218 T5463 T5350 appos tubulin,GFAP
R3219 T5464 T5463 punct -,tubulin
R3220 T5465 T5463 punct ", ",tubulin
R3221 T5466 T5463 npadvmod 1,tubulin
R3222 T5467 T5468 punct :,"15,000"
R3223 T5468 T5466 prep "15,000",1
R3224 T5469 T5470 punct (,Sigma
R3225 T5470 T5463 parataxis Sigma,tubulin
R3226 T5471 T5470 punct ),Sigma
R3227 T5472 T5350 punct ;,GFAP
R3228 T5473 T5350 appos grp75,GFAP
R3229 T5474 T5473 punct ", ",grp75
R3230 T5475 T5473 npadvmod 1,grp75
R3231 T5476 T5477 punct :,200
R3232 T5477 T5475 prep 200,1
R3233 T5478 T5479 punct (,Stressgen
R3234 T5479 T5473 parataxis Stressgen,grp75
R3235 T5480 T5479 punct ),Stressgen
R3236 T5481 T5350 punct ;,GFAP
R3237 T5482 T5483 amod early,antigen
R3238 T5483 T5350 appos antigen,GFAP
R3239 T5484 T5483 compound endosome,antigen
R3240 T5485 T5483 punct -,antigen
R3241 T5486 T5483 nummod 1,antigen
R3242 T5487 T5483 punct (,antigen
R3243 T5488 T5483 appos EEA1,antigen
R3244 T5489 T5483 punct ),antigen
R3245 T5490 T5483 punct ", ",antigen
R3246 T5491 T5492 nummod 2,μg
R3247 T5492 T5483 npadvmod μg,antigen
R3248 T5493 T5494 punct /,ml
R3249 T5494 T5492 prep ml,μg
R3250 T5495 T5496 punct (,Biotechnology
R3251 T5496 T5483 parataxis Biotechnology,antigen
R3252 T5497 T5496 compound Santa,Biotechnology
R3253 T5498 T5496 compound Cruz,Biotechnology
R3254 T5499 T5496 punct ),Biotechnology
R3255 T5500 T5350 punct ;,GFAP
R3256 T5501 T5350 appos rab,GFAP
R3257 T5502 T5501 nummod 7,rab
R3258 T5503 T5501 punct ", ",rab
R3259 T5504 T5505 nummod 4,μg
R3260 T5505 T5501 npadvmod μg,rab
R3261 T5506 T5507 punct /,ml
R3262 T5507 T5505 prep ml,μg
R3263 T5508 T5509 punct (,Biotechnology
R3264 T5509 T5501 parataxis Biotechnology,rab
R3265 T5510 T5509 compound Santa,Biotechnology
R3266 T5511 T5509 compound Cruz,Biotechnology
R3267 T5512 T5509 punct ),Biotechnology
R3268 T5513 T5346 punct .,were
R3269 T5515 T5516 det All,antibodies
R3270 T5516 T5519 nsubjpass antibodies,obtained
R3271 T5517 T5516 amod fluorescent,antibodies
R3272 T5518 T5516 amod secondary,antibodies
R3273 T5520 T5519 auxpass were,obtained
R3274 T5521 T5519 prep from,obtained
R3275 T5522 T5523 compound Jackson,Laboratories
R3276 T5523 T5521 pobj Laboratories,from
R3277 T5524 T5523 compound ImmunoResearch,Laboratories
R3278 T5525 T5519 cc and,obtained
R3279 T5526 T5527 npadvmod HRP,conjugated
R3280 T5527 T5529 amod conjugated,antibodies
R3281 T5528 T5527 punct -,conjugated
R3282 T5529 T5531 nsubjpass antibodies,obtained
R3283 T5530 T5529 amod secondary,antibodies
R3284 T5531 T5519 conj obtained,obtained
R3285 T5532 T5531 auxpass were,obtained
R3286 T5533 T5531 prep from,obtained
R3287 T5534 T5535 compound Amersham,Biosciences
R3288 T5535 T5533 pobj Biosciences,from
R3289 T5536 T5531 punct .,obtained
R3290 T5538 T5539 amod Additional,markers
R3291 T5539 T5541 nsubj markers,were
R3292 T5540 T5539 compound cell,markers
R3293 T5542 T5543 mark as,follows
R3294 T5543 T5541 advcl follows,were
R3295 T5544 T5541 punct : ,were
R3296 T5545 T5546 compound VVL,biotin
R3297 T5546 T5541 dep biotin,were
R3298 T5547 T5546 punct -,biotin
R3299 T5548 T5546 punct ", ",biotin
R3300 T5549 T5546 npadvmod 1,biotin
R3301 T5550 T5551 punct :,2000
R3302 T5551 T5549 prep 2000,1
R3303 T5552 T5553 punct (,Laboratories
R3304 T5553 T5546 parataxis Laboratories,biotin
R3305 T5554 T5553 compound Vector,Laboratories
R3306 T5555 T5553 punct ),Laboratories
R3307 T5556 T5546 punct ", ",biotin
R3308 T5557 T5558 nummod "10,000",MW
R3309 T5558 T5559 compound MW,FITC
R3310 T5559 T5546 conj FITC,biotin
R3311 T5560 T5559 compound dextran,FITC
R3312 T5561 T5559 punct -,FITC
R3313 T5562 T5559 punct ", ",FITC
R3314 T5563 T5564 nummod 1,mg
R3315 T5564 T5559 npadvmod mg,FITC
R3316 T5565 T5566 punct /,ml
R3317 T5566 T5564 prep ml,mg
R3318 T5567 T5559 cc and,FITC
R3319 T5568 T5569 compound Lysotracker,DND
R3320 T5569 T5559 conj DND,FITC
R3321 T5570 T5569 compound Red,DND
R3322 T5571 T5569 punct -,DND
R3323 T5572 T5569 nummod 99,DND
R3324 T5573 T5569 punct ", ",DND
R3325 T5574 T5575 nummod 500,nM
R3326 T5575 T5569 npadvmod nM,DND
R3327 T5576 T5577 punct (,Probes
R3328 T5577 T5569 parataxis Probes,DND
R3329 T5578 T5577 compound Molecular,Probes
R3330 T5579 T5577 punct ),Probes
R3331 T5580 T5541 punct .,were
R3332 T5714 T5713 punct ", ",Generation
R3333 T5715 T5713 conj maintenance,Generation
R3334 T5716 T5715 cc and,maintenance
R3335 T5717 T5715 conj differentiation,maintenance
R3336 T5718 T5713 prep of,Generation
R3337 T5719 T5720 nmod CbCln3,lines
R3338 T5720 T5718 pobj lines,of
R3339 T5721 T5720 amod cerebellar,lines
R3340 T5722 T5723 amod neuronal,precursor
R3341 T5723 T5720 compound precursor,lines
R3342 T5725 T5726 nmod Cln3Δex7,mice
R3343 T5726 T5732 nsubjpass mice,described
R3344 T5727 T5725 punct /,Cln3Δex7
R3345 T5728 T5725 nummod 8,Cln3Δex7
R3346 T5729 T5726 amod knock,mice
R3347 T5730 T5729 punct -,knock
R3348 T5731 T5729 prt in,knock
R3349 T5733 T5732 aux have,described
R3350 T5734 T5732 auxpass been,described
R3351 T5735 T5732 advmod previously,described
R3352 T5736 T5737 punct [,12
R3353 T5737 T5732 parataxis 12,described
R3354 T5738 T5737 punct ],12
R3355 T5739 T5732 punct .,described
R3356 T5741 T5742 compound Littermate,pups
R3357 T5742 T5743 nsubjpass pups,used
R3358 T5744 T5742 prep from,pups
R3359 T5745 T5746 compound heterozygote,crosses
R3360 T5746 T5744 pobj crosses,from
R3361 T5747 T5743 auxpass were,used
R3362 T5748 T5743 prep for,used
R3363 T5749 T5750 amod primary,establishment
R3364 T5750 T5748 pobj establishment,for
R3365 T5751 T5750 compound culture,establishment
R3366 T5752 T5743 punct ", ",used
R3367 T5753 T5743 prep by,used
R3368 T5754 T5755 advmod previously,described
R3369 T5755 T5756 amod described,methods
R3370 T5756 T5753 pobj methods,by
R3371 T5757 T5758 dep that,yield
R3372 T5758 T5756 relcl yield,methods
R3373 T5759 T5760 amod cerebellar,neuron
R3374 T5760 T5762 npadvmod neuron,enriched
R3375 T5761 T5760 compound granule,neuron
R3376 T5762 T5764 amod enriched,cultures
R3377 T5763 T5762 punct -,enriched
R3378 T5764 T5758 dobj cultures,yield
R3379 T5765 T5766 punct [,26
R3380 T5766 T5758 parataxis 26,yield
R3381 T5767 T5766 punct ],26
R3382 T5768 T5743 punct .,used
R3383 T5770 T5771 amod Postnatal,day
R3384 T5771 T5772 nmod day,cerebella
R3385 T5772 T5777 nsubjpass cerebella,dissected
R3386 T5773 T5771 nummod 4,day
R3387 T5774 T5771 punct (,day
R3388 T5775 T5771 appos P4,day
R3389 T5776 T5772 punct ),cerebella
R3390 T5778 T5777 auxpass were,dissected
R3391 T5779 T5777 prep in,dissected
R3392 T5780 T5781 poss Hank,Solution
R3393 T5781 T5779 pobj Solution,in
R3394 T5782 T5780 case 's,Hank
R3395 T5783 T5781 compound Balanced,Solution
R3396 T5784 T5781 compound Salt,Solution
R3397 T5785 T5781 punct (,Solution
R3398 T5786 T5781 appos HBSS,Solution
R3399 T5787 T5788 punct ", ",Sigma
R3400 T5788 T5781 parataxis Sigma,Solution
R3401 T5789 T5788 punct ),Sigma
R3402 T5790 T5781 punct ", ",Solution
R3403 T5791 T5781 acl supplemented,Solution
R3404 T5792 T5791 prep with,supplemented
R3405 T5793 T5794 nummod 35,mM
R3406 T5794 T5795 compound mM,glucose
R3407 T5795 T5792 pobj glucose,with
R3408 T5796 T5777 punct .,dissected
R3409 T5798 T5799 compound Tail,biopsies
R3410 T5799 T5800 nsubjpass biopsies,collected
R3411 T5801 T5800 auxpass were,collected
R3412 T5802 T5800 advmod also,collected
R3413 T5803 T5800 prep for,collected
R3414 T5804 T5805 amod genomic,isolation
R3415 T5805 T5803 pobj isolation,for
R3416 T5806 T5805 compound DNA,isolation
R3417 T5807 T5805 cc and,isolation
R3418 T5808 T5809 amod genotypic,analysis
R3419 T5809 T5805 conj analysis,isolation
R3420 T5810 T5800 punct .,collected
R3421 T5812 T5813 compound Trypsin,EDTA
R3422 T5813 T5815 nsubj EDTA,helped
R3423 T5814 T5813 punct /,EDTA
R3424 T5816 T5817 punct (,Sigma
R3425 T5817 T5813 parataxis Sigma,EDTA
R3426 T5818 T5819 nummod 10,mg
R3427 T5819 T5817 dep mg,Sigma
R3428 T5820 T5821 punct /,ml
R3429 T5821 T5819 prep ml,mg
R3430 T5822 T5817 punct ", ",Sigma
R3431 T5823 T5817 punct ),Sigma
R3432 T5824 T5813 cc and,EDTA
R3433 T5825 T5813 conj DNase,EDTA
R3434 T5826 T5825 nummod I,DNase
R3435 T5827 T5828 punct (,Sigma
R3436 T5828 T5825 parataxis Sigma,DNase
R3437 T5829 T5830 nummod 100,μg
R3438 T5830 T5828 dep μg,Sigma
R3439 T5831 T5832 punct /,ml
R3440 T5832 T5830 prep ml,μg
R3441 T5833 T5828 punct ", ",Sigma
R3442 T5834 T5828 punct ),Sigma
R3443 T5835 T5813 punct ", ",EDTA
R3444 T5836 T5813 acl suspended,EDTA
R3445 T5837 T5836 prep in,suspended
R3446 T5838 T5837 pobj HBSS,in
R3447 T5839 T5815 punct ", ",helped
R3448 T5840 T5815 xcomp dissociate,helped
R3449 T5841 T5840 dobj cerebella,dissociate
R3450 T5842 T5840 prep for,dissociate
R3451 T5843 T5844 amod primary,culture
R3452 T5844 T5845 compound culture,plating
R3453 T5845 T5842 pobj plating,for
R3454 T5846 T5845 prep onto,plating
R3455 T5847 T5848 nummod 0.01,%
R3456 T5848 T5849 compound %,poly-ornithine
R3457 T5849 T5850 npadvmod poly-ornithine,coated
R3458 T5850 T5854 amod coated,dishes
R3459 T5851 T5852 punct (,Sigma
R3460 T5852 T5849 parataxis Sigma,poly-ornithine
R3461 T5853 T5852 punct ),Sigma
R3462 T5854 T5846 pobj dishes,onto
R3463 T5855 T5856 nummod 100,mm
R3464 T5856 T5854 compound mm,dishes
R3465 T5857 T5815 punct .,helped
R3466 T5859 T5860 amod Primary,cultures
R3467 T5860 T5861 nsubjpass cultures,cultured
R3468 T5862 T5860 prep from,cultures
R3469 T5863 T5864 amod individual,cerebella
R3470 T5864 T5862 pobj cerebella,from
R3471 T5865 T5861 auxpass were,cultured
R3472 T5866 T5861 advmod overnight,cultured
R3473 T5867 T5861 prep at,cultured
R3474 T5868 T5869 nummod 37,°C
R3475 T5869 T5867 pobj °C,at
R3476 T5870 T5869 punct ", ",°C
R3477 T5871 T5872 nummod 5,%
R3478 T5872 T5873 compound %,CO2
R3479 T5873 T5869 appos CO2,°C
R3480 T5874 T5861 punct ", ",cultured
R3481 T5875 T5861 prep in,cultured
R3482 T5876 T5877 compound granule,neuron
R3483 T5877 T5878 compound neuron,media
R3484 T5878 T5875 pobj media,in
R3485 T5879 T5878 compound growth,media
R3486 T5880 T5878 punct (,media
R3487 T5881 T5882 poss Dulbecco,Medium
R3488 T5882 T5878 appos Medium,media
R3489 T5883 T5881 case 's,Dulbecco
R3490 T5884 T5882 compound Modified,Medium
R3491 T5885 T5882 compound Eagle,Medium
R3492 T5886 T5882 punct [,Medium
R3493 T5887 T5882 appos DMEM,Medium
R3494 T5888 T5882 punct ", ",Medium
R3495 T5889 T5890 compound Gibco,#
R3496 T5890 T5882 appos #,Medium
R3497 T5891 T5890 compound BRL,#
R3498 T5892 T5890 nummod 11995,#
R3499 T5893 T5890 punct -,#
R3500 T5894 T5890 nummod 065,#
R3501 T5895 T5882 punct ],Medium
R3502 T5896 T5882 punct ", ",Medium
R3503 T5897 T5898 nummod 10,%
R3504 T5898 T5882 appos %,Medium
R3505 T5899 T5900 amod fetal,serum
R3506 T5900 T5898 appos serum,%
R3507 T5901 T5900 amod bovine,serum
R3508 T5902 T5900 punct [,serum
R3509 T5903 T5904 compound Sigma,F
R3510 T5904 T5900 appos F,serum
R3511 T5905 T5904 compound #,F
R3512 T5906 T5904 punct -,F
R3513 T5907 T5904 nummod 2442,F
R3514 T5908 T5882 punct ],Medium
R3515 T5909 T5882 punct ", ",Medium
R3516 T5910 T5882 acl supplemented,Medium
R3517 T5911 T5910 prep with,supplemented
R3518 T5912 T5913 nummod 24,mM
R3519 T5913 T5914 compound mM,KCl
R3520 T5914 T5911 pobj KCl,with
R3521 T5915 T5861 punct ),cultured
R3522 T5916 T5861 punct .,cultured
R3523 T5918 T5919 nsubjpass Infection,performed
R3524 T5920 T5919 auxpass was,performed
R3525 T5921 T5922 det the,day
R3526 T5922 T5919 npadvmod day,performed
R3527 T5923 T5922 amod following,day
R3528 T5924 T5919 prep with,performed
R3529 T5925 T5926 amod defective,retrovirus
R3530 T5926 T5927 nsubj retrovirus,transducing
R3531 T5927 T5924 pcomp transducing,with
R3532 T5928 T5929 det the,antigen
R3533 T5929 T5927 dobj antigen,transducing
R3534 T5930 T5931 npadvmod temperature,sensitive
R3535 T5931 T5929 amod sensitive,antigen
R3536 T5932 T5931 punct -,sensitive
R3537 T5933 T5934 nmod tsA58,U19
R3538 T5934 T5929 nmod U19,antigen
R3539 T5935 T5934 punct /,U19
R3540 T5936 T5929 amod large,antigen
R3541 T5937 T5929 compound T,antigen
R3542 T5938 T5929 cc and,antigen
R3543 T5939 T5940 det a,cassette
R3544 T5940 T5929 conj cassette,antigen
R3545 T5941 T5940 compound selection,cassette
R3546 T5942 T5943 compound neomycin,resistance
R3547 T5943 T5940 compound resistance,cassette
R3548 T5944 T5943 punct -,resistance
R3549 T5945 T5946 punct [,27
R3550 T5946 T5940 parataxis 27,cassette
R3551 T5947 T5946 punct ],27
R3552 T5948 T5927 punct ", ",transducing
R3553 T5949 T5950 mark as,described
R3554 T5950 T5927 advcl described,transducing
R3555 T5951 T5950 advmod previously,described
R3556 T5952 T5953 punct [,28
R3557 T5953 T5950 parataxis 28,described
R3558 T5954 T5953 punct ],28
R3559 T5955 T5919 punct .,performed
R3560 T5957 T5958 prep Following,shifted
R3561 T5959 T5957 pobj infection,Following
R3562 T5960 T5958 punct ", ",shifted
R3563 T5961 T5958 nsubjpass cultures,shifted
R3564 T5962 T5958 auxpass were,shifted
R3565 T5963 T5958 prep to,shifted
R3566 T5964 T5965 det the,temperature
R3567 T5965 T5963 pobj temperature,to
R3568 T5966 T5967 compound tsA58,U19
R3569 T5967 T5969 npadvmod U19,permissive
R3570 T5968 T5967 punct /,U19
R3571 T5969 T5965 amod permissive,temperature
R3572 T5970 T5965 compound growth,temperature
R3573 T5971 T5965 prep of,temperature
R3574 T5972 T5973 nummod 33,°C
R3575 T5973 T5971 pobj °C,of
R3576 T5974 T5958 cc and,shifted
R3577 T5975 T5976 nsubj selection,was
R3578 T5976 T5958 conj was,shifted
R3579 T5977 T5976 prep in,was
R3580 T5978 T5979 det the,media
R3581 T5979 T5977 pobj media,in
R3582 T5980 T5979 amod same,media
R3583 T5981 T5979 compound growth,media
R3584 T5982 T5979 prep as,media
R3585 T5983 T5982 pcomp above,as
R3586 T5984 T5976 punct ", ",was
R3587 T5985 T5976 prep with,was
R3588 T5986 T5987 nummod 200,μg
R3589 T5987 T5988 nmod μg,G418
R3590 T5988 T5985 pobj G418,with
R3591 T5989 T5990 punct /,ml
R3592 T5990 T5987 prep ml,μg
R3593 T5991 T5976 punct .,was
R3594 T5993 T5994 advmod Conditionally,immortalized
R3595 T5994 T5995 amod immortalized,colonies
R3596 T5995 T5996 nsubjpass colonies,isolated
R3597 T5997 T5996 auxpass were,isolated
R3598 T5998 T5999 quantmod 3,9
R3599 T5999 T6001 nummod 9,weeks
R3600 T6000 T5999 punct –,9
R3601 T6001 T6002 npadvmod weeks,post-infection
R3602 T6002 T5996 advmod post-infection,isolated
R3603 T6003 T6004 cc and,expanded
R3604 T6004 T5996 dep expanded,isolated
R3605 T6005 T6004 prep for,expanded
R3606 T6006 T6007 amod frozen,stocks
R3607 T6007 T6005 pobj stocks,for
R3608 T6008 T6007 cc and,stocks
R3609 T6009 T6010 amod further,isolation
R3610 T6010 T6007 conj isolation,stocks
R3611 T6011 T6010 compound sub-clone,isolation
R3612 T6012 T5996 punct .,isolated
R3613 T6014 T6015 amod Multiple,lines
R3614 T6015 T6017 nsubjpass lines,obtained
R3615 T6016 T6015 amod clonal,lines
R3616 T6018 T6017 auxpass were,obtained
R3617 T6019 T6017 prep for,obtained
R3618 T6020 T6021 det each,genotype
R3619 T6021 T6019 pobj genotype,for
R3620 T6022 T6021 cc and,genotype
R3621 T6023 T6024 det all,phenotypes
R3622 T6024 T6021 conj phenotypes,genotype
R3623 T6025 T6026 auxpass were,confirmed
R3624 T6026 T6017 dep confirmed,obtained
R3625 T6027 T6026 prep in,confirmed
R3626 T6028 T6029 advmod at,2
R3627 T6029 T6031 nummod 2,lines
R3628 T6030 T6029 advmod least,2
R3629 T6031 T6027 pobj lines,in
R3630 T6032 T6031 amod independent,lines
R3631 T6033 T6031 compound CbCln3,lines
R3632 T6034 T6031 compound cell,lines
R3633 T6035 T6017 punct .,obtained
R3634 T6037 T6038 compound CbCln3,lines
R3635 T6038 T6040 nsubjpass lines,maintained
R3636 T6039 T6038 compound cell,lines
R3637 T6041 T6040 auxpass were,maintained
R3638 T6042 T6040 prep on,maintained
R3639 T6043 T6044 nummod 0.01,%
R3640 T6044 T6045 nmod %,dishes
R3641 T6045 T6042 pobj dishes,on
R3642 T6046 T6047 compound poly,ornithine
R3643 T6047 T6049 npadvmod ornithine,coated
R3644 T6048 T6047 punct -,ornithine
R3645 T6049 T6045 amod coated,dishes
R3646 T6050 T6040 prep at,maintained
R3647 T6051 T6052 quantmod 30,90
R3648 T6052 T6054 nummod 90,%
R3649 T6053 T6052 punct –,90
R3650 T6054 T6055 compound %,confluency
R3651 T6055 T6050 pobj confluency,at
R3652 T6056 T6040 punct ", ",maintained
R3653 T6057 T6040 prep in,maintained
R3654 T6058 T6059 nummod 33,°C
R3655 T6059 T6057 pobj °C,in
R3656 T6060 T6059 cc and,°C
R3657 T6061 T6062 nummod 5,atmosphere
R3658 T6062 T6059 conj atmosphere,°C
R3659 T6063 T6061 punct %,5
R3660 T6064 T6062 compound CO2,atmosphere
R3661 T6065 T6040 punct .,maintained
R3662 T6067 T6068 compound Passage,number
R3663 T6068 T6069 nsubjpass number,recorded
R3664 T6070 T6069 auxpass was,recorded
R3665 T6071 T6072 punct (,passages
R3666 T6072 T6069 parataxis passages,recorded
R3667 T6073 T6074 quantmod up,20
R3668 T6074 T6072 nummod 20,passages
R3669 T6075 T6074 quantmod to,20
R3670 T6076 T6074 punct ~,20
R3671 T6077 T6072 punct ),passages
R3672 T6078 T6069 punct ", ",recorded
R3673 T6079 T6069 cc but,recorded
R3674 T6080 T6069 conj had,recorded
R3675 T6081 T6082 det no,impact
R3676 T6082 T6080 dobj impact,had
R3677 T6083 T6082 amod apparent,impact
R3678 T6084 T6082 prep on,impact
R3679 T6085 T6084 pobj phenotype,on
R3680 T6086 T6069 punct .,recorded
R3681 T6088 T6089 amod Neuronal,differentiation
R3682 T6089 T6090 nsubj differentiation,was
R3683 T6091 T6092 mark as,described
R3684 T6092 T6090 advcl described,was
R3685 T6093 T6092 advmod previously,described
R3686 T6094 T6095 punct [,29
R3687 T6095 T6092 parataxis 29,described
R3688 T6096 T6095 punct ],29
R3689 T6097 T6090 prep with,was
R3690 T6098 T6099 det the,cocktail
R3691 T6099 T6097 pobj cocktail,with
R3692 T6100 T6099 amod following,cocktail
R3693 T6101 T6099 punct : ,cocktail
R3694 T6102 T6103 nummod 10,ng
R3695 T6103 T6104 nmod ng,FGF
R3696 T6104 T6099 appos FGF,cocktail
R3697 T6105 T6106 punct /,ml
R3698 T6106 T6103 prep ml,ng
R3699 T6107 T6104 compound α,FGF
R3700 T6108 T6104 punct -,FGF
R3701 T6109 T6104 punct ", ",FGF
R3702 T6110 T6111 nummod 250,μM
R3703 T6111 T6112 compound μM,IBMX
R3704 T6112 T6104 appos IBMX,FGF
R3705 T6113 T6104 punct ", ",FGF
R3706 T6114 T6115 nummod 200,nM
R3707 T6115 T6116 compound nM,TPA
R3708 T6116 T6104 appos TPA,FGF
R3709 T6117 T6104 punct ", ",FGF
R3710 T6118 T6119 nummod 50,μM
R3711 T6119 T6120 compound μM,forskolin
R3712 T6120 T6104 appos forskolin,FGF
R3713 T6121 T6104 punct ", ",FGF
R3714 T6122 T6123 nummod 5,μM
R3715 T6123 T6124 compound μM,dopamine
R3716 T6124 T6104 appos dopamine,FGF
R3717 T6125 T6126 punct (,Sigma
R3718 T6126 T6104 parataxis Sigma,FGF
R3719 T6127 T6126 punct ),Sigma
R3720 T6128 T6090 punct .,was
R3721 T6166 T6165 cc and,Genotyping
R3722 T6167 T6168 compound RT,PCR
R3723 T6168 T6165 conj PCR,Genotyping
R3724 T6169 T6168 punct -,PCR
R3725 T6171 T6172 amod Genomic,DNA
R3726 T6172 T6173 nsubjpass DNA,extracted
R3727 T6174 T6173 auxpass was,extracted
R3728 T6175 T6173 prep from,extracted
R3729 T6176 T6177 compound tail,biopsies
R3730 T6177 T6175 pobj biopsies,from
R3731 T6178 T6177 cc and,biopsies
R3732 T6179 T6180 compound cell,pellets
R3733 T6180 T6177 conj pellets,biopsies
R3734 T6181 T6182 mark as,described
R3735 T6182 T6173 advcl described,extracted
R3736 T6183 T6184 punct (,Cotman
R3737 T6184 T6173 meta Cotman,extracted
R3738 T6185 T6184 nmod et,Cotman
R3739 T6186 T6184 nmod al.,Cotman
R3740 T6187 T6184 punct ", ",Cotman
R3741 T6188 T6184 nummod 2002,Cotman
R3742 T6189 T6184 punct ),Cotman
R3743 T6190 T6173 punct .,extracted
R3744 T6192 T6193 nmod Cln3Δex7,allele
R3745 T6193 T6199 compound allele,genotyping
R3746 T6194 T6192 punct /,Cln3Δex7
R3747 T6195 T6192 nummod 8,Cln3Δex7
R3748 T6196 T6193 amod knock,allele
R3749 T6197 T6196 punct -,knock
R3750 T6198 T6196 prt in,knock
R3751 T6199 T6201 nsubj genotyping,was
R3752 T6200 T6199 compound PCR,genotyping
R3753 T6202 T6201 prep with,was
R3754 T6203 T6204 amod wild,type
R3755 T6204 T6206 compound type,primers
R3756 T6205 T6204 punct -,type
R3757 T6206 T6202 pobj primers,with
R3758 T6207 T6206 punct ", ",primers
R3759 T6208 T6206 appos WtF,primers
R3760 T6209 T6208 punct (,WtF
R3761 T6210 T6211 nummod 5,CAGCATCTCCTCAGGGCTA
R3762 T6211 T6208 appos CAGCATCTCCTCAGGGCTA,WtF
R3763 T6212 T6210 punct ',5
R3764 T6213 T6211 punct -,CAGCATCTCCTCAGGGCTA
R3765 T6214 T6211 punct -,CAGCATCTCCTCAGGGCTA
R3766 T6215 T6211 nummod 3,CAGCATCTCCTCAGGGCTA
R3767 T6216 T6211 punct ',CAGCATCTCCTCAGGGCTA
R3768 T6217 T6208 punct ),WtF
R3769 T6218 T6208 cc and,WtF
R3770 T6219 T6208 conj WtR,WtF
R3771 T6220 T6219 punct (,WtR
R3772 T6221 T6222 nummod 5,CCAACATAGAAAGTAGGGTGTGC
R3773 T6222 T6219 appos CCAACATAGAAAGTAGGGTGTGC,WtR
R3774 T6223 T6221 punct ',5
R3775 T6224 T6222 punct -,CCAACATAGAAAGTAGGGTGTGC
R3776 T6225 T6222 punct -,CCAACATAGAAAGTAGGGTGTGC
R3777 T6226 T6222 nummod 3,CCAACATAGAAAGTAGGGTGTGC
R3778 T6227 T6222 punct ',CCAACATAGAAAGTAGGGTGTGC
R3779 T6228 T6201 punct ),was
R3780 T6229 T6230 aux to,yield
R3781 T6230 T6201 advcl yield,was
R3782 T6231 T6232 det a,band
R3783 T6232 T6230 dobj band,yield
R3784 T6233 T6234 punct ~,250
R3785 T6234 T6235 nummod 250,bp
R3786 T6235 T6232 compound bp,band
R3787 T6236 T6201 cc and,was
R3788 T6237 T6238 amod knock,primers
R3789 T6238 T6201 conj primers,was
R3790 T6239 T6237 punct -,knock
R3791 T6240 T6237 prt in,knock
R3792 T6241 T6238 punct ", ",primers
R3793 T6242 T6238 appos 552F,primers
R3794 T6243 T6242 punct (,552F
R3795 T6244 T6245 nummod 5,GAGCTTTGTTCTGGTTGCCTTC
R3796 T6245 T6242 appos GAGCTTTGTTCTGGTTGCCTTC,552F
R3797 T6246 T6244 punct ',5
R3798 T6247 T6245 punct -,GAGCTTTGTTCTGGTTGCCTTC
R3799 T6248 T6245 punct -,GAGCTTTGTTCTGGTTGCCTTC
R3800 T6249 T6245 nummod 3,GAGCTTTGTTCTGGTTGCCTTC
R3801 T6250 T6245 punct ',GAGCTTTGTTCTGGTTGCCTTC
R3802 T6251 T6242 punct ),552F
R3803 T6252 T6242 cc and,552F
R3804 T6253 T6242 conj Ex9RA,552F
R3805 T6254 T6253 punct (,Ex9RA
R3806 T6255 T6256 nummod 5,GCAGTCTCTGCCTCGTTTTCT
R3807 T6256 T6253 appos GCAGTCTCTGCCTCGTTTTCT,Ex9RA
R3808 T6257 T6255 punct ',5
R3809 T6258 T6256 punct -,GCAGTCTCTGCCTCGTTTTCT
R3810 T6259 T6256 punct -,GCAGTCTCTGCCTCGTTTTCT
R3811 T6260 T6256 nummod 3,GCAGTCTCTGCCTCGTTTTCT
R3812 T6261 T6256 punct ',GCAGTCTCTGCCTCGTTTTCT
R3813 T6262 T6238 punct ),primers
R3814 T6263 T6264 aux to,yield
R3815 T6264 T6238 advcl yield,primers
R3816 T6265 T6266 det a,band
R3817 T6266 T6264 dobj band,yield
R3818 T6267 T6268 punct ~,500
R3819 T6268 T6269 nummod 500,bp
R3820 T6269 T6266 compound bp,band
R3821 T6270 T6201 punct .,was
R3822 T6272 T6273 compound PCR,cycling
R3823 T6273 T6274 compound cycling,conditions
R3824 T6274 T6275 nsubj conditions,were
R3825 T6276 T6277 nummod 95,°C
R3826 T6277 T6275 attr °C,were
R3827 T6278 T6277 prep for,°C
R3828 T6279 T6280 nummod 30,seconds
R3829 T6280 T6278 pobj seconds,for
R3830 T6281 T6277 punct ", ",°C
R3831 T6282 T6283 nummod 58,°C
R3832 T6283 T6277 conj °C,°C
R3833 T6284 T6283 prep for,°C
R3834 T6285 T6286 nummod 30,seconds
R3835 T6286 T6284 pobj seconds,for
R3836 T6287 T6283 punct ", ",°C
R3837 T6288 T6283 cc and,°C
R3838 T6289 T6290 nummod 72,°C
R3839 T6290 T6283 conj °C,°C
R3840 T6291 T6290 prep for,°C
R3841 T6292 T6293 nummod 35,seconds
R3842 T6293 T6291 pobj seconds,for
R3843 T6294 T6277 punct ", ",°C
R3844 T6295 T6277 acl repeated,°C
R3845 T6296 T6295 prep for,repeated
R3846 T6297 T6298 nummod 34,cycles
R3847 T6298 T6296 pobj cycles,for
R3848 T6299 T6275 punct .,were
R3849 T6301 T6302 amod Total,isolation
R3850 T6302 T6304 nsubjpass isolation,described
R3851 T6303 T6302 compound RNA,isolation
R3852 T6305 T6302 cc and,isolation
R3853 T6306 T6307 compound Cln3,primers
R3854 T6307 T6302 conj primers,isolation
R3855 T6308 T6309 compound RT,PCR
R3856 T6309 T6307 compound PCR,primers
R3857 T6310 T6309 punct -,PCR
R3858 T6311 T6307 cc and,primers
R3859 T6312 T6307 conj procedures,primers
R3860 T6313 T6304 aux have,described
R3861 T6314 T6304 auxpass been,described
R3862 T6315 T6304 advmod previously,described
R3863 T6316 T6317 punct [,12
R3864 T6317 T6304 parataxis 12,described
R3865 T6318 T6317 punct ],12
R3866 T6319 T6304 punct .,described
R3877 T6382 T6381 compound chamber,slides
R3878 T6383 T6381 punct -,slides
R3879 T6384 T6385 punct (,Falcon
R3880 T6385 T6381 parataxis Falcon,slides
R3881 T6386 T6385 punct ),Falcon
R3882 T6387 T6375 prep at,seeded
R3883 T6388 T6389 det a,density
R3884 T6389 T6387 pobj density,at
R3885 T6390 T6389 prep of,density
R3886 T6391 T6392 quantmod 5,104
R3887 T6392 T6394 nummod 104,cells
R3888 T6393 T6392 punct ×,104
R3889 T6394 T6390 pobj cells,of
R3890 T6395 T6394 prep per,cells
R3891 T6396 T6395 pobj well,per
R3892 T6397 T6375 prep for,seeded
R3893 T6398 T6399 compound microscopy,studies
R3894 T6399 T6397 pobj studies,for
R3895 T6400 T6375 punct ", ",seeded
R3896 T6401 T6375 cc or,seeded
R3897 T6402 T6375 conj into,seeded
R3898 T6403 T6404 nummod 100,mm
R3899 T6404 T6405 compound mm,dishes
R3900 T6405 T6402 pobj dishes,into
R3901 T6406 T6407 punct (,Falcon
R3902 T6407 T6405 parataxis Falcon,dishes
R3903 T6408 T6407 punct ),Falcon
R3904 T6409 T6402 prep at,into
R3905 T6410 T6411 det a,density
R3906 T6411 T6409 pobj density,at
R3907 T6412 T6411 prep of,density
R3908 T6413 T6414 quantmod 5,105
R3910 T6415 T6414 punct ×,105
R3935 T6442 T6444 npadvmod day,post-confluency
R3937 T6444 T6439 advmod post-confluency,considered
R3938 T6445 T6439 punct .,considered
R3940 T6449 T6450 det the,times
R3941 T6450 T6447 pobj times,At
R3942 T6451 T6450 amod indicated,times
R3943 T6452 T6448 punct ", ",fixed
R6234 T9854 T9853 punct -,type
R6236 T9856 T9853 punct (,type
R6237 T9857 T9853 appos CbCln3,type
R6238 T9858 T9857 punct +,CbCln3
R6239 T9859 T9857 punct /,CbCln3
R6240 T9860 T9857 punct +,CbCln3
R6241 T9861 T9862 punct ", ",panel
R6273 T9893 T9891 pobj vesicles,with
R6275 T9895 T9896 advmod also,present
R5192 T8129 T8130 meta c,magnification
R5193 T8130 T8122 appos magnification,magnification
R5194 T8131 T8129 punct ", ",c
R5518 T8654 T8655 amod Wild,type
R5844 T9272 T9270 punct .,a
R2930 T5053 T5048 conj deficits,trafficking
R2931 T5054 T5048 acl uncovered,trafficking
R2932 T5055 T5054 prep in,uncovered
R2933 T5056 T5057 amod homozygous,cells
R2934 T5057 T5055 pobj cells,in
R2935 T5058 T5057 nmod CbCln3Δex7,cells
R2936 T5059 T5058 punct /,CbCln3Δex7
R2937 T5060 T5058 nummod 8,CbCln3Δex7
R2938 T5061 T5050 acomp likely,are
R2939 T5062 T5063 aux to,impact
R2940 T5063 T5061 xcomp impact,likely
R2941 T5064 T5063 advmod particularly,impact
R2942 T5065 T5066 amod neuronal,function
R2943 T5066 T5063 dobj function,impact
R2944 T5067 T5050 punct .,are
R2945 T5069 T5070 nsubj Neurotransmission,relies
R2946 T5071 T5070 advmod heavily,relies
R2947 T5072 T5070 prep on,relies
R2948 T5073 T5074 compound membrane,vesicle
R2949 T5074 T5075 compound vesicle,transport
R2950 T5075 T5072 pobj transport,on
R2951 T5076 T5070 punct ", ",relies
R2952 T5077 T5070 cc and,relies
R1687 T2879 T2880 amod enzymatic,activity
R1688 T2880 T2878 pobj activity,on
R1689 T2881 T2874 auxpass was,tested
R1690 T2882 T2874 advmod next,tested
R1691 T2883 T2884 aux to,determine
R1692 T2884 T2874 advcl determine,tested
R1693 T2885 T2886 mark if,accounts
R1694 T2886 T2884 ccomp accounts,determine
R6220 T9838 T9834 pobj cells,in
R6225 T9843 T9838 nmod CbCln3Δex7,cells
R6226 T9844 T9843 punct /,CbCln3Δex7
R6227 T9845 T9843 nummod 8,CbCln3Δex7
R6228 T9846 T9838 compound precursor,cells
R6229 T9847 T9817 auxpass is,shown
R6230 T9848 T9817 punct .,shown
R6231 T9850 T9851 prep In,observed
R6232 T9852 T9853 amod wild,type
R6233 T9853 T9855 nmod type,cells
R6242 T9862 T9853 parataxis panel,type
R6243 T9863 T9862 amod left,panel
R6244 T9864 T9862 punct ),panel
R6245 T9865 T9853 cc and,type
R6246 T9866 T9853 conj heterozygous,type
R6247 T9867 T9868 punct (,panel
R1695 T2887 T2888 amod altered,activity
R5056 T7982 T7983 amod Neuronal,marker
R5057 T7983 T7984 compound marker,expression
R5058 T7984 T7985 dep expression,shown
R5059 T7986 T7984 prep in,expression
R5060 T7987 T7988 nmod CbCln3,cells
R5061 T7988 T7986 pobj cells,in
R5062 T7989 T7987 punct +,CbCln3
R5116 T8047 T8035 nsubj cells,achieved
R5118 T8049 T8046 punct /,CbCln3
R5119 T8050 T8046 punct +,CbCln3
R6059 T9591 T9587 pobj lysosomes,of
R6061 T9593 T9589 conj homozygous,type
R6062 T9594 T9591 nmod CbCln3Δex7,lysosomes
R6063 T9595 T9594 punct /,CbCln3Δex7
R6064 T9596 T9594 nummod 8,CbCln3Δex7
R6065 T9597 T9598 amod Lysosomal,labeling
R6066 T9598 T9586 nsubjpass labeling,shown
R6067 T9599 T9598 prep of,labeling
R6068 T9600 T9601 amod wild,type
R6069 T9601 T9603 nmod type,cells
R6070 T9602 T9601 punct -,type
R6071 T9603 T9599 pobj cells,of
R6072 T9604 T9601 cc and,type
R6073 T9605 T9606 amod homozygous,CbCln3Δex7
R6074 T9606 T9601 conj CbCln3Δex7,type
R6075 T9607 T9606 punct /,CbCln3Δex7
R6076 T9608 T9606 nummod 8,CbCln3Δex7
R6077 T9609 T9603 compound precursor,cells
R6078 T9610 T9598 prep with,labeling
R6079 T9611 T9612 nmod lysotracker,antibody
R6080 T9612 T9610 pobj antibody,with
R6081 T9613 T9611 cc and,lysotracker
R6082 T9614 T9611 conj Lamp,lysotracker
R6083 T9615 T9614 nummod 2,Lamp
R6084 T9616 T9586 auxpass is,shown
R6085 T9617 T9586 punct .,shown
R6086 T9619 T9620 compound Lysotracker,dye
R6087 T9620 T9621 nsubj dye,labeled
R6088 T9622 T9623 punct (,panels
R6089 T9623 T9620 parataxis panels,dye
R1696 T2888 T2886 nsubj activity,accounts
R6103 T9637 T9635 pobj periphery,in
R6104 T9638 T9637 prep of,periphery
R6105 T9639 T9640 amod wild,type
R6106 T9640 T9642 compound type,cells
R6107 T9641 T9640 punct -,type
R4450 T7116 T7117 compound Cathepsin,D
R4451 T7117 T7118 compound D,assay
R4452 T7119 T7118 compound activity,assay
R4492 T7161 T7157 punct ", ",Tris
R4493 T7162 T7163 nummod 0.1,%
R4494 T7163 T7164 compound %,X
R4495 T7164 T7157 appos X,Tris
R4496 T7165 T7164 compound Triton,X
R4497 T7166 T7164 punct -,X
R4498 T7167 T7164 nummod 100,X
R4499 T7168 T7157 acl followed,Tris
R4500 T7169 T7168 agent by,followed
R4501 T7170 T7169 pobj incubation,by
R4502 T7171 T7170 prep on,incubation
R4503 T7172 T7171 pobj ice,on
R4504 T7173 T7170 punct ", ",incubation
R4505 T7174 T7170 prep for,incubation
R4506 T7175 T7176 nummod 20,minutes
R4507 T7176 T7174 pobj minutes,for
R4508 T7177 T7149 punct .,isolated
R4509 T7179 T7180 det The,material
R4511 T7181 T7180 amod insoluble,material
R4510 T7180 T7182 nsubjpass material,centrifuged
R4519 T7190 T7182 conj isolated,centrifuged
R4521 T7192 T7190 punct ", ",isolated
R4522 T7193 T7190 cc and,isolated
R3867 T6369 T6370 compound Subunit,c
R3868 T6370 T6371 compound c,accumulation
R1697 T2889 T2888 amod enzymatic,activity
R3869 T6371 T6372 compound accumulation,assay
R3870 T6374 T6375 nsubjpass Cells,seeded
R3871 T6376 T6375 auxpass were,seeded
R3872 T6377 T6375 prep into,seeded
R3873 T6378 T6379 nummod 4,well
R4594 T7269 T7248 dep 35,measured
R4595 T7270 T7269 punct ", ",35
R4596 T7271 T7269 nummod 360,35
R4597 T7272 T7269 punct -,35
R4598 T7273 T7269 punct ;,35
R4599 T7274 T7275 compound emission,filter
R4600 T7275 T7276 dep filter,20
R4601 T7276 T7269 conj 20,35
R3874 T6379 T6381 compound well,slides
R3875 T6380 T6379 punct -,well
R3876 T6381 T6377 pobj slides,into
R3909 T6414 T6416 nummod 105,cells
R3911 T6416 T6412 pobj cells,of
R3912 T6417 T6416 prep per,cells
R3913 T6418 T6417 pobj dish,per
R3914 T6419 T6402 prep for,into
R3915 T6420 T6421 compound protein,extraction
R3916 T6421 T6419 pobj extraction,for
R3917 T6422 T6375 punct .,seeded
R3918 T6424 T6425 nsubj Cells,were
R3919 T6426 T6425 advmod typically,were
R3920 T6427 T6428 punct >,95
R3921 T6428 T6429 nummod 95,%
R3922 T6429 T6430 npadvmod %,confluent
R3923 T6430 T6425 acomp confluent,were
R3924 T6431 T6432 nummod one,day
R3925 T6432 T6433 npadvmod day,post-plating
R3926 T6433 T6425 advmod post-plating,were
R3927 T6434 T6425 punct ", ",were
R3928 T6435 T6425 cc and,were
R3929 T6436 T6437 det the,day
R3930 T6437 T6439 nsubjpass day,considered
R3931 T6438 T6437 amod following,day
R3932 T6439 T6425 conj considered,were
R3933 T6440 T6439 auxpass was,considered
R3934 T6441 T6442 nummod 1,day
R1698 T2890 T2886 prep for,accounts
R3936 T6443 T6442 punct -,day
R1699 T2891 T2892 amod inefficient,turnover
R2060 T3636 T3637 nmod ste,t
R2061 T3637 T3635 pobj t,i
R4002 T6513 T6510 npadvmod pH,buffer
R4003 T6514 T6513 nummod 7.4,pH
R4004 T6515 T6495 prep for,fixed
R4005 T6516 T6517 nummod 1,hour
R4019 T6530 T6529 punct ],12
R4006 T6517 T6515 pobj hour,for
R4020 T6531 T6495 punct .,fixed
R4007 T6518 T6495 cc and,fixed
R3939 T6447 T6448 prep At,fixed
R4008 T6519 T6520 advmod subsequently,post-fixed
R4009 T6520 T6495 conj post-fixed,fixed
R708 T1294 T1293 compound cell,culture
R707 T1293 T1285 compound culture,system
R4010 T6521 T6520 cc and,post-fixed
R4011 T6522 T6520 conj processed,post-fixed
R4012 T6523 T6522 prep for,processed
R4013 T6524 T6525 compound TEM,analysis
R4014 T6525 T6523 pobj analysis,for
R4015 T6526 T6527 mark as,described
R4016 T6527 T6522 advcl described,processed
R4017 T6528 T6529 punct [,12
R2062 T3638 T3636 cc n,ste
R4018 T6529 T6527 parataxis 12,described
R713 T1299 T1298 acl cultured,neurons
R3944 T6453 T6448 nsubjpass cells,fixed
R3945 T6454 T6448 auxpass were,fixed
R3946 T6455 T6448 advmod either,fixed
R3947 T6456 T6448 prep with,fixed
R3948 T6457 T6458 nummod 4,%
R3949 T6458 T6459 compound %,formaldehyde
R3950 T6459 T6456 pobj formaldehyde,with
R3951 T6460 T6448 prep in,fixed
R3952 T6461 T6462 nmod phosphate,saline
R3953 T6462 T6460 pobj saline,in
R3954 T6463 T6462 amod buffered,saline
R3955 T6464 T6462 punct (,saline
R3956 T6465 T6462 appos PBS,saline
R3957 T6466 T6462 punct ),saline
R3958 T6467 T6462 punct ", ",saline
R3959 T6468 T6462 npadvmod pH,saline
R3960 T6469 T6468 nummod 7.4,pH
R3961 T6470 T6448 punct ", ",fixed
R3962 T6471 T6448 prep for,fixed
R3963 T6472 T6473 nummod 20,minutes
R3964 T6473 T6471 pobj minutes,for
R3965 T6474 T6448 cc and,fixed
R4021 T6533 T6534 prep In,observed
R4022 T6535 T6536 amod confocal,studies
R4023 T6536 T6533 pobj studies,In
R4024 T6537 T6536 compound microscopy,studies
R4025 T6538 T6534 punct ", ",observed
R4026 T6539 T6540 amod autofluorescent,signal
R4027 T6540 T6534 nsubjpass signal,observed
R4028 T6541 T6534 auxpass was,observed
R4029 T6542 T6534 prep over,observed
R4030 T6543 T6544 amod multiple,wavelengths
R4031 T6544 T6542 pobj wavelengths,over
R4032 T6545 T6534 punct .,observed
R4033 T6547 T6548 prep For,reduced
R4034 T6549 T6547 pobj co-staining,For
R4035 T6550 T6548 punct ", ",reduced
R4036 T6551 T6548 nsubjpass settings,reduced
R4037 T6552 T6548 auxpass were,reduced
R4038 T6553 T6548 advcl such,reduced
R4039 T6554 T6555 mark that,contribute
R4041 T6556 T6557 amod autofluorescent,signal
R4040 T6555 T6553 ccomp contribute,such
R4042 T6557 T6555 nsubj signal,contribute
R4043 T6558 T6555 aux did,contribute
R4044 T6559 T6555 neg not,contribute
R4045 T6560 T6555 prep to,contribute
R4046 T6561 T6562 compound antibody,signal
R4047 T6562 T6560 pobj signal,to
R4048 T6563 T6562 compound label,signal
R4049 T6564 T6548 punct .,reduced
R5321 T8445 T8446 nmod Battenin,marker
R5323 T8447 T8445 cc and,Battenin
R5324 T8448 T8445 conj lysosomal,Battenin
R5322 T8446 T8451 compound marker,co-staining
R5325 T8449 T8448 cc and,lysosomal
R5326 T8450 T8448 conj endosomal,lysosomal
R5327 T8451 T8452 dep co-staining,shown
R5328 T8453 T8451 prep in,co-staining
R5329 T8454 T8455 amod wild,type
R5330 T8455 T8457 nmod type,cells
R5331 T8456 T8455 punct -,type
R5332 T8457 T8453 pobj cells,in
R5333 T8458 T8455 cc and,type
R5334 T8459 T8455 conj homozygous,type
R5335 T8460 T8461 nmod CbCln3Δex7,precursor
R5336 T8461 T8457 compound precursor,cells
R5337 T8462 T8460 punct /,CbCln3Δex7
R5338 T8463 T8460 nummod 8,CbCln3Δex7
R5339 T8464 T8461 amod cerebellar,precursor
R5340 T8465 T8466 compound Batp1,immunostaining
R5341 T8466 T8452 nsubjpass immunostaining,shown
R5342 T8467 T8466 prep of,immunostaining
R5355 T8480 T8469 conj cells,type
R5505 T8640 T8641 compound Rab7,staining
R5506 T8641 T8642 nsubj staining,was
R5507 T8642 T8630 conj was,appear
R5508 T8643 T8642 advmod frequently,was
R5509 T8644 T8645 advmod less,intense
R5510 T8645 T8642 acomp intense,was
R5511 T8646 T8642 prep in,was
R5512 T8647 T8648 amod homozygous,cells
R5513 T8648 T8646 pobj cells,in
R5514 T8649 T8648 nmod CbCln3Δex7,cells
R5515 T8650 T8649 punct /,CbCln3Δex7
R5516 T8651 T8649 nummod 8,CbCln3Δex7
R5517 T8652 T8642 punct .,was
R5519 T8655 T8657 nsubjpass type,captured
R5520 T8656 T8655 punct -,type
R5521 T8658 T8655 cc and,type
R5522 T8659 T8660 amod homozygous,CbCln3Δex7
R5523 T8660 T8661 nmod CbCln3Δex7,images
R5524 T8661 T8655 conj images,type
R5525 T8662 T8660 punct /,CbCln3Δex7
R5526 T8663 T8660 nummod 8,CbCln3Δex7
R5527 T8664 T8661 amod confocal,images
R5528 T8665 T8657 auxpass were,captured
R5529 T8666 T8657 prep with,captured
R5530 T8667 T8668 amod identical,settings
R5531 T8668 T8666 pobj settings,with
R5532 T8669 T8668 compound exposure,settings
R5533 T8670 T8657 punct .,captured
R5534 T8672 T8673 nummod 60,magnification
R5535 T8674 T8672 punct ×,60
R5536 T8675 T8673 punct .,magnification
R5829 T9254 T9255 compound Cathepsin,D
R5830 T9256 T9255 appos localization,D
R5831 T9257 T9256 cc and,localization
R5832 T9258 T9256 conj processing,localization
R5833 T9259 T9255 prep in,D
R5837 T9263 T9259 pobj cells,in
R5842 T9268 T9266 nummod 8,CbCln3Δex7
R5843 T9270 T9271 meta a,shown
R5845 T9273 T9271 nsubjpass Immunostaining,shown
R5846 T9274 T9273 prep of,Immunostaining
R5847 T9275 T9276 amod wild,type
R5848 T9276 T9278 nmod type,cells
R5849 T9277 T9276 punct -,type
R5850 T9278 T9274 pobj cells,of
R5851 T9279 T9276 cc and,type
R5852 T9280 T9276 conj homozygous,type
R5853 T9281 T9278 nmod CbCln3Δex7,cells
R5854 T9282 T9281 punct /,CbCln3Δex7
R5855 T9283 T9281 nummod 8,CbCln3Δex7
R5856 T9284 T9278 compound precursor,cells
R5857 T9285 T9273 prep with,Immunostaining
R5858 T9286 T9287 amod anti-cathepsin,D
R5859 T9287 T9288 amod D,antibody
R5860 T9288 T9285 pobj antibody,with
R5861 T9289 T9273 punct ", ",Immunostaining
R5937 T9373 T9371 pobj tissue,of
R5941 T9377 T9375 cc versus,type
R5942 T9378 T9379 amod homozygous,Cln3Δex7
R5943 T9379 T9380 npadvmod Cln3Δex7,knock
R5944 T9380 T9375 conj knock,type
R5945 T9381 T9379 punct /,Cln3Δex7
R5946 T9382 T9379 nummod 8,Cln3Δex7
R5947 T9383 T9380 punct -,knock
R5948 T9384 T9380 prt in,knock
R5949 T9385 T9373 cc or,tissue
R5950 T9386 T9387 nmod CbCln3Δex7,extracts
R5951 T9387 T9373 conj extracts,tissue
R5952 T9388 T9386 punct /,CbCln3Δex7
R5953 T9389 T9386 nummod 8,CbCln3Δex7
R5954 T9390 T9387 amod cellular,extracts
R5955 T9391 T9362 auxpass are,shown
R5956 T9392 T9362 punct .,shown
R5957 T9394 T9395 det The,band
R5958 T9395 T9401 nsubj band,was
R2925 T5047 T5048 det The,trafficking
R2926 T5048 T5050 nsubj trafficking,are
R2927 T5049 T5048 compound membrane,trafficking
R2928 T5051 T5048 cc and,trafficking
R2929 T5052 T5053 amod mitochondrial,deficits
R717 T1303 T1300 pobj cerebella,from
R759 T1347 T1341 conj antigen,cassette
R13 T182 T180 pobj model,in
R31 T206 T208 advmod commonly,caused
R43 T219 T221 nsubj loss,leads
R52 T229 T227 pobj synthase,of
R59 T236 T226 conj loss,deposition
R88 T267 T269 amod like,pathology
R168 T353 T348 dobj c,accumulate
R204 T391 T381 conj reduced,altered
R215 T404 T406 nsubjpass levels,decreased
R217 T406 T396 conj decreased,are
R231 T424 T422 ccomp is,reveal
R236 T429 T427 pobj trafficking,for
R258 T453 T437 conj impact,are
R1643 T2811 T2812 compound enzyme,secretion
R1644 T2812 T2813 nsubjpass secretion,affected
R1645 T2813 T2810 advcl affected,indicating
R1646 T2814 T2813 auxpass was,affected
R1647 T2815 T2813 neg not,affected
R1648 T2816 T2801 punct .,contain
R1649 T2818 T2819 amod Heterozygous,mice
R1650 T2819 T2823 nsubj mice,were
R1651 T2820 T2819 nmod Cln3Δex7,mice
R1652 T2821 T2820 punct /,Cln3Δex7
R1653 T2822 T2820 nummod 8,Cln3Δex7
R1654 T2824 T2819 cc and,mice
R1655 T2825 T2826 nmod CbCln3Δex7,cells
R1656 T2826 T2819 conj cells,mice
R1657 T2827 T2825 punct /,CbCln3Δex7
R1658 T2828 T2825 nummod 8,CbCln3Δex7
R1659 T2829 T2823 acomp indistinguishable,were
R1660 T2830 T2829 prep from,indistinguishable
R1661 T2831 T2832 amod wild,type
R1662 T2832 T2830 pobj type,from
R1663 T2833 T2832 punct -,type
R1664 T2834 T2823 punct ", ",were
R1665 T2835 T2836 mark as,expected
R1666 T2836 T2823 advcl expected,were
R1667 T2837 T2836 prep for,expected
R1668 T2838 T2839 det a,phenotype
R1669 T2839 T2837 pobj phenotype,for
R1670 T2840 T2839 amod recessive,phenotype
R1671 T2841 T2839 compound disease,phenotype
R1672 T2842 T2843 punct (,shown
R1673 T2843 T2836 parataxis shown,expected
R1674 T2844 T2843 nsubj data,shown
R1675 T2845 T2843 neg not,shown
R1676 T2846 T2843 punct ),shown
R1677 T2847 T2823 punct .,were
R1678 T2855 T2856 amod c,t
R1679 T2862 T2863 amod e,a
R1680 T2865 T2866 advmod l,ed
R1681 T2867 T2866 nsubj er,ed
R1682 T2868 T2867 nmod t,er
R1683 T2873 T2874 nsubjpass p,tested
R1684 T2876 T2877 compound sin,D
R1685 T2877 T2875 compound D,processing
R1686 T2878 T2873 prep on,p
R310 T743 T738 parataxis chromosomes,defect
R320 T753 T741 attr deletion,is
R515 T960 T950 dobj model,generated
R562 T1011 T1009 dobj disease,exhibit
R595 T1046 T1044 ccomp leads,suggest
R1172 T2052 T2053 amod Heterozygous,cells
R1173 T2053 T2057 nsubj cells,contained
R1174 T2054 T2053 nmod CbCln3Δex7,cells
R1175 T2055 T2054 punct /,CbCln3Δex7
R1176 T2056 T2054 nummod 8,CbCln3Δex7
R1177 T2058 T2059 det a,mixture
R1178 T2059 T2057 dobj mixture,contained
R1179 T2060 T2059 prep of,mixture
R1180 T2061 T2062 compound Cln3,products
R1181 T2062 T2060 pobj products,of
R1182 T2063 T2062 compound mRNA,products
R1183 T2064 T2059 prep from,mixture
R1184 T2065 T2066 preconj both,allele
R1185 T2066 T2064 pobj allele,from
R1186 T2067 T2066 det the,allele
R1187 T2068 T2069 amod wild,type
R1188 T2069 T2066 compound type,allele
R1189 T2070 T2069 punct -,type
R1190 T2071 T2066 cc and,allele
R1191 T2072 T2073 det the,allele
R1192 T2073 T2066 conj allele,allele
R1193 T2074 T2073 compound mutant,allele
R1194 T2075 T2057 punct ", ",contained
R1195 T2076 T2057 cc and,contained
R1196 T2077 T2078 compound batp1,signal
R1197 T2078 T2079 nsubj signal,was
R1198 T2079 T2057 conj was,contained
R1199 T2080 T2079 acomp similar,was
R1200 T2081 T2080 prep to,similar
R1201 T2082 T2081 pobj that,to
R1202 T2083 T2082 acl seen,that
R1203 T2084 T2083 prep in,seen
R1204 T2085 T2086 amod wild,type
R1205 T2086 T2088 compound type,cells
R1206 T2087 T2086 punct -,type
R1207 T2088 T2084 pobj cells,in
R1208 T2089 T2090 punct (,shown
R1209 T2090 T2079 parataxis shown,was
R1210 T2091 T2090 nsubj data,shown
R1214 T2096 T2097 prep During,displayed
R1215 T2098 T2099 amod sub-confluent,conditions
R1216 T2099 T2096 pobj conditions,During
R1217 T2100 T2099 compound growth,conditions
R1218 T2101 T2097 punct ", ",displayed
R1219 T2102 T2103 preconj neither,type
R1220 T2103 T2106 nmod type,cells
R1221 T2104 T2103 amod wild,type
R1222 T2105 T2103 punct -,type
R1223 T2106 T2097 nsubj cells,displayed
R1224 T2107 T2103 cc nor,type
R1225 T2108 T2109 amod homozygous,CbCln3Δex7
R1226 T2109 T2103 conj CbCln3Δex7,type
R1227 T2110 T2109 punct /,CbCln3Δex7
R1228 T2111 T2109 nummod 8,CbCln3Δex7
R1229 T2112 T2113 nmod autofluorescence,formation
R1230 T2113 T2097 dobj formation,displayed
R1231 T2114 T2112 cc or,autofluorescence
R1232 T2115 T2116 compound subunit,c
R1233 T2116 T2117 compound c,inclusion
R1234 T2117 T2112 conj inclusion,autofluorescence
R1235 T2118 T2119 punct (,shown
R1236 T2119 T2097 parataxis shown,displayed
R1237 T2120 T2119 nsubj data,shown
R1238 T2121 T2119 neg not,shown
R1239 T2122 T2119 punct ),shown
R1983 T3550 T3552 nmod type,cells
R1985 T3552 T3548 pobj cells,in
R1986 T3553 T3550 cc and,type
R1987 T3554 T3550 conj homozygous,type
R1988 T3555 T3552 nmod CbCln3Δex7,cells
R1989 T3556 T3555 punct /,CbCln3Δex7
R1990 T3557 T3555 nummod 8,CbCln3Δex7
R1991 T3558 T3537 prep by,were
R2057 T3632 T3631 nmod C,o
R2058 T3633 T3630 nsubjpass n,w
R2059 T3635 T3633 prep i,n
R2063 T3640 T3641 amod i,t
R2064 T3645 T3646 amod h,e
R2065 T3663 T3661 amod o,me
R2066 T3669 T3668 amod r,signal
R2067 T3670 T3668 nmod ker,signal
R2068 T3671 T3670 punct (,ker
R2069 T3672 T3670 appos EEA1,ker
R2070 T3673 T3668 punct ),signal
R2071 T3674 T3668 acl observed,signal
R2072 T3675 T3674 prep by,observed
R2073 T3676 T3675 pobj immunostaining,by
R2074 T3677 T3678 punct (,Fig.
R2075 T3679 T3678 nummod 3,Fig.
R2076 T3680 T3678 punct ),Fig.
R2077 T3681 T3667 punct ", ",altered
R2078 T3682 T3683 compound fluid,phase
R2160 T3768 T3767 prep of,cytoplasm
R2161 T3769 T3770 det the,cell
R2162 T3770 T3768 pobj cell,of
R2163 T3771 T3744 punct .,stained
R2164 T3778 T3776 nmod l,l
R2165 T3780 T3779 punct ", ",y
R2166 T3792 T3793 amod u,n
R2167 T3793 T3794 nmod n,c
R2168 T3808 T3807 amod hondrial,protein
R2169 T3810 T3811 poss its,turnover
R2170 T3811 T3812 nsubj turnover,proceeds
R2171 T3813 T3812 prep through,proceeds
R2172 T3814 T3815 amod autophagic,engulfment
R2173 T3815 T3813 pobj engulfment,through
R2174 T3816 T3815 prep of,engulfment
R2175 T3817 T3816 pobj mitochondria,of
R2176 T3818 T3819 punct [,13
R2177 T3819 T3812 parataxis 13,proceeds
R2178 T3820 T3819 punct ],13
R2179 T3821 T3806 punct ", ",analyzed
R2180 T3822 T3806 nsubj we,analyzed
R2181 T3823 T3824 amod homozygous,CbCln3Δex7
R2182 T3824 T3806 dobj CbCln3Δex7,analyzed
R2183 T3825 T3824 punct /,CbCln3Δex7
R2184 T3826 T3824 nummod 8,CbCln3Δex7
R2185 T3827 T3828 npadvmod cell,mitochondrial
R2186 T3828 T3824 amod mitochondrial,CbCln3Δex7
R2851 T4919 T4920 amod late,endosomes
R2852 T4920 T4918 pobj endosomes,with
R2853 T4921 T4920 cc and,endosomes
R2854 T4922 T4920 conj lysosomes,endosomes
R2855 T4923 T4916 prep in,overlap
R2856 T4924 T4925 amod non-neuronal,cells
R2857 T4925 T4923 pobj cells,in

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T102 0-8 GO:0016020 denotes Membrane
T103 0-20 GO:0055085 denotes Membrane trafficking
T104 25-38 GO:0005739 denotes mitochondrial
T105 61-70 PR_EXT:000022190 denotes subunit c
T106 87-97 UBERON:0002037 denotes cerebellar
T107 98-102 CL_GO_EXT:cell denotes cell
T108 112-120 PATO_UBERON_EXT:juvenile_organism denotes juvenile
T109 121-129 CL:0000540 denotes neuronal
T110 206-215 UBERON_EXT:childhood denotes childhood
T111 278-279 CHEBI_SO_EXT:base denotes b
T112 280-284 PR_EXT:000005591 denotes CLN3
T113 285-293 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T114 317-325 PR_EXT:000005591 denotes battenin
T115 358-371 GO:0005739 denotes mitochondrial
T116 358-395 PR_EXT:000022190 denotes mitochondrial ATP synthase, subunit c
T117 372-375 CHEBI_EXT:ATP denotes ATP
T118 419-422 UBERON:0001017 denotes CNS
T119 419-430 CL:0000117 denotes CNS neurons
T120 456-460 PR_EXT:000005591 denotes Cln3
T121 476-480 NCBITaxon:10088 denotes mice
T122 514-522 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T123 524-531 GO:0010467 denotes express
T124 532-538 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T125 539-547 PR_EXT:000005591 denotes battenin
T126 639-647 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T127 651-665 CL:0000540 denotes neuronal cells
T128 660-665 CL_GO_EXT:cell denotes cells
T129 687-692 NCBITaxon:10088 denotes mouse
T130 693-702 UBERON:0002037 denotes cerebella
T131 743-745 UBERON:0002037 denotes Cb
T132 745-749 PR_EXT:000005591 denotes Cln3
T133 750-759 SO_EXT:wild_type_entity_or_quality denotes wild-type
T134 790-798 CL:0000540 denotes neuronal
T135 809-813 CL_GO_EXT:cell denotes cell
T136 834-848 GO_PATO_RO_EXT:developmental_differentiation_process_or_quality denotes differentiated
T137 854-859 PR_EXT:000010124 denotes MAP-2
T138 864-868 PR_EXT:000006252 denotes NeuN
T139 879-885 CL:0000540 denotes neuron
T140 891-896 CL_GO_EXT:cell denotes cells
T141 909-911 UBERON:0002037 denotes Cb
T142 911-915 PR_EXT:000005591 denotes Cln3
T143 932-937 CL_GO_EXT:cell denotes cells
T144 938-945 GO:0010467 denotes express
T145 960-966 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T146 967-975 PR_EXT:000005591 denotes battenin
T147 986-990 GO_PATO_EXT:aging_or_agedness denotes aged
T148 1017-1023 GO_EXT:0016887 denotes ATPase
T149 1017-1033 PR_EXT:000022190 denotes ATPase subunit c
T150 1091-1097 GO_EXT:biological_growth_entity_or_process denotes growth
T151 1099-1110 PR_EXT:000006025 denotes cathepsin D
T152 1111-1120 GO_EXT:biological_routing_or_transport denotes transport
T153 1125-1135 SO_EXT:sequence_alteration_process denotes processing
T154 1158-1164 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T155 1205-1214 GO:0005764 denotes lysosomal
T156 1254-1265 GO:0006897 denotes endocytosis
T157 1291-1303 GO:0005739 denotes mitochondria
T158 1330-1338 CL_GO_EXT:cell denotes cellular
T159 1339-1342 CHEBI_EXT:ATP denotes ATP
T160 1388-1397 MOP:0000568 denotes oxidative
T161 1457-1465 PR_EXT:000005591 denotes battenin
T162 1482-1495 GO:0005622 denotes intracellular
T163 1482-1495 _FRAGMENT denotes intracellular
T164 1505-1516 GO:0046907 denotes trafficking
T165 1487-1495 CL_GO_EXT:cell denotes cellular
T166 1496-1504 GO:0016020 denotes membrane
T167 1496-1516 GO:0055085 denotes membrane trafficking
T168 1521-1534 GO:0005739 denotes mitochondrial
T169 1660-1668 CL:0000540 denotes neuronal
T619 2390-2393 UBERON:0001017 denotes CNS
T620 2390-2401 CL:0000117 denotes CNS neurons
T621 2431-2439 CL:0000540 denotes neuronal
T622 2440-2445 CL_GO_EXT:cell denotes cells
T623 2461-2475 UBERON:0001016 denotes nervous system
T624 2497-2506 PR_EXT:000022190 denotes subunit c
T625 2579-2587 CL:0000540 denotes neuronal
T626 2645-2649 PR_EXT:000005591 denotes CLN3
T627 2650-2657 SO_EXT:sequence_coding_function denotes encoded
T628 2658-2665 CHEBI_PR_EXT:protein denotes protein
T629 2667-2675 PR_EXT:000005591 denotes battenin
T630 2689-2693 PR_EXT:000005591 denotes CLN3
T631 2697-2703 PR_EXT:000005591 denotes cln3 p
T632 2717-2726 SO_EXT:biological_conservation_process_or_quality denotes conserved
T633 2741-2750 GO:0010467 denotes expressed
T634 2763-2771 GO:0016020 denotes membrane
T635 2772-2779 CHEBI_PR_EXT:protein denotes protein
T636 2789-2798 GO_PATO_EXT:biological_localization_process_or_quality denotes localizes
T637 2806-2814 GO:0005764 denotes lysosome
T638 2825-2834 GO:0031982 denotes vesicular
T639 2855-2863 PR_EXT:000005591 denotes Battenin
T640 2919-2923 PR_EXT:Q9US09 denotes btn1
T641 2929-2934 NCBITaxon_EXT:yeast denotes yeast
T642 2935-2939 PR_EXT:000005591 denotes CLN3
T643 2940-2948 SO_EXT:0000855 denotes ortholog
T644 2966-2974 PR_EXT:000005591 denotes battenin
T645 2978-2987 GO:0005764 denotes lysosomal
T646 2988-3002 GO:0006885 denotes pH homeostasis
T647 3007-3017 CHEBI_SO_EXT:amino_acid denotes amino acid
T648 3007-3027 GO:0006865 denotes amino acid transport
T649 3070-3078 PR_EXT:000005591 denotes battenin
T650 3115-3126 SO_EXT:0000704 denotes genetically
T651 3140-3145 NCBITaxon:10088 denotes mouse
T652 3153-3157 PR_EXT:000005591 denotes Cln3
T653 3173-3177 NCBITaxon:10088 denotes mice
T654 3193-3194 CHEBI_SO_EXT:base denotes b
T655 3207-3215 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T656 3220-3227 GO:0010467 denotes express
T657 3234-3243 SO_EXT:sequence_truncation_process denotes truncated
T658 3244-3250 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T659 3251-3259 PR_EXT:000005591 denotes battenin
T660 3260-3267 SO_EXT:0001060 denotes isoform
T661 3292-3302 GO:0042571 denotes antibodies
T662 3315-3325 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T663 3326-3334 CHEBI_SO_EXT:epitope denotes epitopes
T664 3347-3351 PR_EXT:000005591 denotes Cln3
T665 3367-3371 NCBITaxon:10088 denotes mice
T666 3418-3427 UBERON:0012101 denotes perinatal
T667 3422-3427 GO:0007567 denotes natal
T668 3437-3446 PR_EXT:000022190 denotes subunit c
T669 3466-3470 CL_GO_EXT:cell denotes cell
T670 3496-3504 CL:0000540 denotes neuronal
T671 3521-3531 GO_PATO_EXT:biological_behavior denotes behavioral
T672 3627-3640 GO:0005739 denotes mitochondrial
T673 3627-3650 PR_EXT:000022190 denotes mitochondrial subunit c
T674 3693-3696 UBERON:0001017 denotes CNS
T675 3693-3704 CL:0000117 denotes CNS neurons
T676 3738-3751 CL:0000540 denotes neuronal cell
T677 3747-3751 CL_GO_EXT:cell denotes cell
T678 3804-3812 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T679 3816-3826 CHEBI_GO_EXT:biological_process_or_quality_or_role denotes biological
T680 3816-3836 GO:0008150 denotes biological processes
T681 3869-3879 UBERON:0002037 denotes cerebellar
T682 3869-3888 CL:1001611 denotes cerebellar neuronal
T683 3899-3903 CL_GO_EXT:cell denotes cell
T684 3915-3919 PR_EXT:000005591 denotes Cln3
T685 3935-3939 NCBITaxon:10088 denotes mice
T686 3952-3954 UBERON:0002037 denotes Cb
T687 3954-3958 PR_EXT:000005591 denotes Cln3
T688 3965-3970 CL_GO_EXT:cell denotes cells
T689 4034-4053 GO:0043227 denotes membrane organelles
T690 4063-4071 GO:0016020 denotes membrane
T691 4063-4083 GO:0055085 denotes membrane trafficking
T692 4096-4109 GO:0005739 denotes mitochondrial
T693 4136-4142 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T694 4143-4145 UBERON:0002037 denotes Cb
T695 4156-4161 CL_GO_EXT:cell denotes cells
T1222 4189-4200 SO_EXT:0000704 denotes genetically
T1223 4209-4219 UBERON:0002037 denotes cerebellar
T1224 4225-4229 CL_GO_EXT:cell denotes cell
T1225 4258-4265 SO_EXT:0000704 denotes genetic
T1226 4267-4273 CL:0000540 denotes neuron
T1227 4287-4291 CL_GO_EXT:cell denotes cell
T1228 4324-4339 CL:0000120 denotes granule neurons
T1229 4358-4363 GO:0007567 denotes natal
T1230 4375-4384 UBERON:0002037 denotes cerebella
T1231 4416-4420 PR_EXT:000005591 denotes Cln3
T1232 4436-4440 NCBITaxon:10088 denotes mice
T1233 4446-4455 SO_EXT:wild_type_entity_or_quality denotes wild-type
T1234 4469-4481 CL:0000001 denotes Primary cell
T1235 4477-4481 CL_GO_EXT:cell denotes cell
T1236 4504-4519 CL:0000120 denotes granule neurons
T1237 4525-4535 GO:0009293 denotes transduced
T1238 4541-4551 NCBITaxon:11632 denotes retroviral
T1239 4552-4558 SO_EXT:0000440 denotes vector
T1240 4579-4587 SO_EXT:0005853 denotes cassette
T1241 4620-4624 NCBITaxon:10633 denotes SV40
T1242 4633-4640 CHEBI_EXT:59132 denotes antigen
T1243 4642-4648 GO_EXT:biological_growth_entity_or_process denotes Growth
T1244 4652-4656 CHEBI:42768 denotes G418
T1245 4760-4766 SO_EXT:sequence_cloned_entity denotes clonal
T1246 4767-4773 PR_EXT:000011141 denotes nestin
T1247 4798-4802 PR_EXT:000007939 denotes GFAP
T1248 4823-4827 CL_GO_EXT:cell denotes cell
T1249 4843-4851 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T1250 4856-4864 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T1251 4903-4911 CL_GO_EXT:cell denotes cellular
T1252 4983-4987 NCBITaxon:10633 denotes SV40
T1253 4996-5003 CHEBI_EXT:59132 denotes antigen
T1254 5004-5014 GO:0010467 denotes expression
T1255 5036-5040 CL_GO_EXT:cell denotes cell
T1256 5036-5049 GO:0051301 denotes cell division
T1257 5062-5067 CL_GO_EXT:cell denotes cells
T1258 5157-5165 CL:0000540 denotes neuronal
T1259 5166-5181 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T1260 5182-5190 CHEBI:60004 denotes cocktail
T1261 5202-5207 CL_GO_EXT:cell denotes cells
T1262 5215-5221 CL:0000540 denotes neuron
T1263 5265-5271 PR_EXT:000011141 denotes nestin
T1264 5272-5282 GO:0010467 denotes expression
T1265 5314-5318 PR_EXT:000010124 denotes MAP2
T1266 5323-5327 PR_EXT:000006252 denotes NeuN
T1267 5328-5338 GO:0010467 denotes expression
T1268 5361-5371 GO:0010467 denotes expression
T1269 5388-5394 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T1270 5396-5405 PR_EXT:000004967 denotes calbindin
T1674 6366-6370 PR_EXT:000005591 denotes Cln3
T1662 6156-6158 UBERON:0002037 denotes Cb
T1663 6158-6162 PR_EXT:000005591 denotes Cln3
T1664 6169-6174 CL_GO_EXT:cell denotes cells
T1665 6175-6182 GO:0010467 denotes express
T1666 6183-6189 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T1667 6190-6198 PR_EXT:000005591 denotes battenin
T1668 6242-6244 UBERON:0002037 denotes Cb
T1669 6245-6249 PR_EXT:000005591 denotes Cln3
T1670 6256-6261 CL_GO_EXT:cell denotes cells
T1671 6324-6328 PR_EXT:000005591 denotes Cln3
T1672 6344-6348 NCBITaxon:10088 denotes mice
T1673 6349-6356 GO:0010467 denotes express
T1675 6371-6375 CHEBI_SO_EXT:mRNA denotes mRNA
T1676 6376-6382 GO:0008380 denotes splice
T1677 6376-6391 SO_EXT:alternative_splice_variant denotes splice variants
T1678 6396-6402 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T1679 6403-6411 PR_EXT:000005591 denotes battenin
T1680 6412-6419 CHEBI_PR_EXT:protein denotes protein
T1681 6448-6456 GO:0042571 denotes antibody
T1702 6907-6911 PR_EXT:000005591 denotes Cln3
T1703 6918-6923 CL_GO_EXT:cell denotes cells
T1704 6953-6964 GO:0005737 denotes cytoplasmic
T1705 6966-6975 GO:0031982 denotes vesicular
T1706 7009-7018 SO_EXT:wild_type_entity_or_quality denotes wild-type
T1707 7019-7024 CL_GO_EXT:cell denotes cells
T1708 7071-7080 GO:0005764 denotes lysosomal
T1709 7081-7087 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T1710 7089-7094 PR_EXT:000002060 denotes Lamp1
T1711 7134-7148 GO:0005769 denotes early endosome
T1712 7134-7158 PR_EXT:000006901 denotes early endosome antigen 1
T1713 7149-7156 CHEBI_EXT:59132 denotes antigen
T1714 7160-7164 PR_EXT:000006901 denotes EEA1
T1715 7174-7188 GO:0005770 denotes late endosomal
T1716 7189-7195 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T1717 7197-7201 PR_EXT:000013639 denotes Rab7
T1718 7251-7270 GO:0055037 denotes recycling endosomes
T1719 7289-7300 PR_EXT:000016261 denotes transferrin
T1720 7289-7309 GO_EXT:0004998 denotes transferrin receptor
T1721 7354-7359 PR_EXT:000002060 denotes Lamp1
T1722 7364-7368 PR_EXT:000006901 denotes EEA1
T1723 7428-7430 UBERON:0002037 denotes Cb
T1724 7430-7434 PR_EXT:000005591 denotes Cln3
T1725 7441-7446 CL_GO_EXT:cell denotes cells
T1726 7462-7469 GO:0005634 denotes nuclear
T1727 7489-7498 SO_EXT:wild_type_entity_or_quality denotes wild-type
T1728 7499-7504 CL_GO_EXT:cell denotes cells
T1729 7510-7514 PR_EXT:000013639 denotes Rab7
T1730 7566-7568 UBERON:0002037 denotes Cb
T1731 7568-7572 PR_EXT:000005591 denotes Cln3
T1732 7579-7584 CL_GO_EXT:cell denotes cells
T1733 7608-7610 UBERON:0002037 denotes Cb
T1734 7610-7614 PR_EXT:000005591 denotes Cln3
T1735 7621-7626 CL_GO_EXT:cell denotes cells
T1736 7639-7646 CHEBI:60004 denotes mixture
T1737 7650-7654 PR_EXT:000005591 denotes Cln3
T1738 7655-7659 CHEBI_SO_EXT:mRNA denotes mRNA
T1739 7683-7692 SO_EXT:wild_type_entity_or_quality denotes wild-type
T1740 7693-7699 SO_EXT:0001023 denotes allele
T1741 7708-7714 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T1742 7715-7721 SO_EXT:0001023 denotes allele
T1743 7768-7777 SO_EXT:wild_type_entity_or_quality denotes wild-type
T1744 7778-7783 CL_GO_EXT:cell denotes cells
T2420 11911-11913 UBERON:0002037 denotes Cb
T2421 11913-11917 PR_EXT:000005591 denotes Cln3
T2422 11924-11929 CL_GO_EXT:cell denotes cells
T2423 11934-11938 PR_EXT:000005591 denotes Cln3
T2424 11954-11958 NCBITaxon:10088 denotes mice
T2425 11959-11966 SO_EXT:sequence_alteration_process denotes process
T2426 11967-11978 PR_EXT:000006025 denotes cathepsin D
T2427 12025-12034 PR_EXT:000022190 denotes subunit c
T2428 12077-12088 PR_EXT:000006025 denotes cathepsin D
T2429 12126-12129 CHEBI_EXT:ATP denotes ATP
T2430 12126-12148 PR_EXT:000022190 denotes ATP synthase subunit c
T2431 12168-12176 GO:0005764 denotes lysosome
T2432 12199-12210 PR_EXT:000006025 denotes cathepsin D
T2433 12211-12220 GO_EXT:biological_routing_or_transport denotes transport
T2434 12225-12235 SO_EXT:sequence_alteration_process denotes processing
T2435 12250-12252 UBERON:0002037 denotes Cb
T2436 12252-12256 PR_EXT:000005591 denotes Cln3
T2437 12263-12268 CL_GO_EXT:cell denotes cells
T2438 12273-12277 PR_EXT:000005591 denotes Cln3
T2439 12284-12288 NCBITaxon:10088 denotes mice
T2440 12300-12311 PR_EXT:000006025 denotes cathepsin D
T2441 12312-12320 GO:0042571 denotes antibody
T2442 12339-12348 SO_EXT:sequence_alteration_process denotes processed
T2443 12353-12362 SO_EXT:sequence_alteration_process denotes processed
T2477 13070-13076 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T2478 13112-13124 SO:0000985 denotes double-chain
T2479 13144-13150 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T2480 13171-13173 UBERON:0002037 denotes Cb
T2481 13173-13177 PR_EXT:000005591 denotes Cln3
T2482 13184-13188 CL_GO_EXT:cell denotes cell
T2483 13193-13197 PR_EXT:000005591 denotes Cln3
T2484 13204-13210 UBERON:0000479 denotes tissue
T2485 13321-13330 SO_EXT:wild_type_entity_or_quality denotes wild-type
T2486 13345-13353 CL_GO_EXT:cell denotes cellular
T2487 13354-13360 GO_EXT:biological_growth_entity_or_process denotes growth
T2488 13401-13412 PR_EXT:000006025 denotes cathepsin D
T2489 13425-13431 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T2490 13432-13441 GO_EXT:secretion_entity_or_process denotes secretion
T2491 13473-13477 PR_EXT:000005591 denotes Cln3
T2492 13484-13488 NCBITaxon:10088 denotes mice
T2493 13493-13495 UBERON:0002037 denotes Cb
T2494 13495-13499 PR_EXT:000005591 denotes Cln3
T2495 13506-13511 CL_GO_EXT:cell denotes cells
T2496 13540-13549 SO_EXT:wild_type_entity_or_quality denotes wild-type
T2497 14753-14754 PR_EXT:000006025 denotes i
T2498 14755-14756 SO_EXT:wild_type_entity_or_quality denotes p
T2499 14757-14758 CL_GO_EXT:cell denotes c
T2500 14769-14770 SO_EXT:sequence_altered_entity_or_alteration_process denotes t
T2501 14781-14786 PR_EXT:000006025 denotes sin D
T2502 14787-14797 SO_EXT:sequence_alteration_process denotes processing
T2503 14801-14810 CHEBI_GO_SO_EXT:enzyme denotes enzymatic
T2504 14860-14869 CHEBI_GO_SO_EXT:enzyme denotes enzymatic
T2505 14904-14913 PR_EXT:000022190 denotes subunit c
T2506 14929-14940 CHEBI_EXT:76219 denotes fluorogenic
T2507 14957-14968 PR_EXT:000006025 denotes cathepsin D
T2508 14987-14995 CL_GO_EXT:cell denotes cellular
T2509 15049-15051 UBERON:0002037 denotes Cb
T2510 15051-15055 PR_EXT:000005591 denotes Cln3
T2511 15062-15067 CL_GO_EXT:cell denotes cells
T2512 15091-15098 CHEBI_PR_EXT:protein denotes protein
T2513 15108-15117 SO_EXT:wild_type_entity_or_quality denotes wild-type
T2514 15118-15123 CL_GO_EXT:cell denotes cells
T2515 15147-15154 CHEBI_PR_EXT:protein denotes protein
T2516 15203-15212 CHEBI_GO_SO_EXT:enzyme denotes enzymatic
T2517 15225-15231 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T2518 15232-15237 CL_GO_EXT:cell denotes cells
T2519 15258-15269 PR_EXT:000006025 denotes cathepsin D
T2520 15270-15279 GO_EXT:biological_routing_or_transport denotes transport
T2521 15284-15294 SO_EXT:sequence_alteration_process denotes processing
T2522 15323-15325 UBERON:0002037 denotes Cb
T2523 15325-15329 PR_EXT:000005591 denotes Cln3
T2524 15336-15341 CL_GO_EXT:cell denotes cells
T2525 15364-15373 CHEBI_GO_SO_EXT:enzyme denotes enzymatic
T3213 15595-15597 UBERON:0002037 denotes Cb
T3214 15597-15601 PR_EXT:000005591 denotes Cln3
T3215 15608-15613 CL_GO_EXT:cell denotes cells
T3216 15642-15653 GO_UBERON_EXT:cellular_component_or_cell_part denotes subcellular
T3217 15645-15653 CL_GO_EXT:cell denotes cellular
T3218 15685-15704 GO:0043227 denotes membrane organelles
T3219 15724-15741 GO_EXT:secretory_pathway denotes secretory pathway
T3220 15757-15759 GO:0005783 denotes ER
T3221 15761-15770 GO:0005801 denotes cis-Golgi
T3222 15776-15787 GO:0005802 denotes trans-Golgi
T3223 15817-15826 SO_EXT:wild_type_entity_or_quality denotes wild-type
T3224 15843-15850 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T3225 15871-15878 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T3226 15880-15897 CHEBI:16249 denotes protein disulfide
T3227 15880-15907 GO_PR_EXT:protein_disulfide_isomerase denotes protein disulfide isomerase
T3228 15909-15912 GO_PR_EXT:protein_disulfide_isomerase denotes PDI
T3229 15915-15920 PR_EXT:000008131 denotes GM130
T3230 15965-15974 GO:0005764 denotes lysosomal
T3231 15975-15982 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T3232 16000-16006 PR_EXT:000002061 denotes Lamp 2
T3233 16054-16056 UBERON:0002037 denotes Cb
T3234 16056-16060 PR_EXT:000005591 denotes Cln3
T3235 16067-16072 CL_GO_EXT:cell denotes cells
T3236 16081-16090 SO_EXT:wild_type_entity_or_quality denotes wild-type
T3237 16091-16096 CL_GO_EXT:cell denotes cells
T3238 16098-16107 SO_EXT:wild_type_entity_or_quality denotes Wild-type
T3239 16108-16113 CL_GO_EXT:cell denotes cells
T3240 16141-16150 GO:0005764 denotes lysosomes
T3241 16192-16199 GO:0005634 denotes nuclear
T3242 16226-16228 UBERON:0002037 denotes Cb
T3243 16228-16232 PR_EXT:000005591 denotes Cln3
T3244 16239-16248 GO:0005764 denotes lysosomes
T3245 16279-16287 GO:0031982 denotes vesicles
T3246 16330-16339 GO:0005737 denotes cytoplasm
T3247 16347-16351 CL_GO_EXT:cell denotes cell
T3248 16362-16368 PR_EXT:000002060 denotes Lamp 1
T3249 16439-16445 PR_EXT:000002060 denotes Lamp 1
T3250 16450-16456 PR_EXT:000002061 denotes Lamp 2
T3251 16463-16470 CHEBI_PR_EXT:protein denotes protein
T3252 16494-16503 SO_EXT:wild_type_entity_or_quality denotes wild-type
T3253 16519-16521 UBERON:0002037 denotes Cb
T3254 16521-16525 PR_EXT:000005591 denotes Cln3
T3255 16532-16537 CL_GO_EXT:cell denotes cells
T3256 16618-16627 GO:0005764 denotes lysosomes
T3257 16639-16651 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T3258 16659-16666 CHEBI_SO_EXT:epitope denotes epitope
T3259 16732-16735 CHEBI_EXT:37958 denotes dye
T3260 16770-16776 CHEBI_EXT:37527 denotes acidic
T3261 16830-16839 GO:0005764 denotes lysosomal
T3262 16840-16848 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeling
T3263 16887-16896 GO:0005764 denotes lysosomal
T3264 17823-17824 UBERON:0002037 denotes C
T3265 17824-17825 GO:0005764 denotes o
T3266 17825-17826 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes n
T3267 17832-17833 GO:0042571 denotes t
T3307 19152-19159 CHEBI_PR_EXT:protein denotes protein
T3308 19194-19231 GO:0000422 denotes autophagic engulfment of mitochondria
T3309 19219-19231 GO:0005739 denotes mitochondria
T3310 19261-19263 UBERON:0002037 denotes Cb
T3311 19263-19267 PR_EXT:000005591 denotes Cln3
T3312 19274-19278 CL_GO_EXT:cell denotes cell
T3313 19279-19292 GO:0005739 denotes mitochondrial
T3314 19318-19331 GO:0005739 denotes Mitochondrial
T3315 19359-19361 UBERON:0002037 denotes Cb
T3316 19361-19365 PR_EXT:000005591 denotes Cln3
T3317 19372-19377 CL_GO_EXT:cell denotes cells
T3318 19405-19414 SO_EXT:wild_type_entity_or_quality denotes wild-type
T3319 19432-19437 CL_GO_EXT:cell denotes cells
T3320 19459-19461 UBERON:0002037 denotes Cb
T3321 19461-19465 PR_EXT:000005591 denotes Cln3
T3322 19472-19484 GO:0005739 denotes mitochondria
T3323 19512-19517 PR_EXT:000008813 denotes grp75
T3324 19518-19524 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T3325 19586-19592 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T3326 19593-19605 GO:0005739 denotes mitochondria
T3327 19683-19692 SO_EXT:wild_type_entity_or_quality denotes wild-type
T3328 19693-19705 GO:0005739 denotes mitochondria
T3329 19762-19775 GO:0005739 denotes Mitochondrial
T3330 19812-19814 UBERON:0002037 denotes Cb
T3331 19814-19818 PR_EXT:000005591 denotes Cln3
T3332 19825-19830 CL_GO_EXT:cell denotes cells
T3333 19871-19880 SO_EXT:wild_type_entity_or_quality denotes wild-type
T3334 19897-19902 CL_GO_EXT:cell denotes cells
T3335 19915-19917 UBERON:0002037 denotes Cb
T3336 19917-19921 PR_EXT:000005591 denotes Cln3
T3337 19928-19933 CL_GO_EXT:cell denotes cells
T3338 19960-19968 CL_GO_EXT:cell denotes cellular
T3339 19969-19972 CHEBI_EXT:ATP denotes ATP
T3340 20046-20063 CHEBI:16240 denotes hydrogen peroxide
T3341 20083-20092 SO_EXT:wild_type_entity_or_quality denotes wild-type
T3342 20140-20150 GO:0008152 denotes metabolism
T3343 20155-20164 MOP:0000568 denotes oxidative
T3344 20172-20180 GO_EXT:reaction_or_response denotes response
T3345 20226-20239 GO:0005739 denotes mitochondrial
T3346 20263-20265 UBERON:0002037 denotes Cb
T3347 20265-20269 PR_EXT:000005591 denotes Cln3
T3348 20276-20281 CL_GO_EXT:cell denotes cells
T6623 30918-30923 CL_GO_EXT:cell denotes cells
T6624 30962-30967 CL_GO_EXT:cell denotes cells
T6625 31006-31011 GO:0040007 denotes grown
T6626 31089-31101 CHEBI:16842 denotes formaldehyde
T6627 31151-31159 CHEBI:17790 denotes methanol
T6628 31160-31167 CHEBI:15347 denotes acetone
T6629 31222-31230 GO:0042571 denotes antibody
T6630 31243-31248 CL_GO_EXT:cell denotes Cells
T6631 31377-31384 CHEBI_SO_EXT:glycine denotes glycine
T6632 31422-31430 GO:0042571 denotes antibody
T6633 31442-31454 CHEBI:9750 denotes Triton X-100
T6634 31500-31506 NCBITaxon:27592 denotes bovine
T6635 31507-31512 UBERON:0001977 denotes serum
T6636 31507-31520 PR_EXT:000003918 denotes serum albumin
T6637 31558-31566 GO:0042571 denotes antibody
T6638 31615-31623 GO:0042571 denotes antibody
T6639 31933-31942 SO_EXT:wild_type_entity_or_quality denotes wild-type
T6640 31947-31953 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T6641 31986-31995 SO_EXT:wild_type_entity_or_quality denotes wild-type
T4263 21950-21952 UBERON:0002037 denotes Cb
T4264 21952-21956 PR_EXT:000005591 denotes Cln3
T4265 21963-21973 UBERON:0002037 denotes cerebellar
T4266 21984-21989 CL_GO_EXT:cell denotes cells
T4267 22010-22021 SO_EXT:0000704 denotes genetically
T4268 22031-22037 CL:0000540 denotes neuron
T4269 22080-22082 UBERON:0002037 denotes Cb
T4270 22082-22086 PR_EXT:000005591 denotes Cln3
T4271 22093-22098 CL_GO_EXT:cell denotes cells
T4272 22099-22106 GO:0010467 denotes express
T4273 22107-22113 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T4274 22114-22122 PR_EXT:000005591 denotes battenin
T4275 22141-22154 GO:0005739 denotes mitochondrial
T4276 22141-22171 PR_EXT:000022190 denotes mitochondrial ATPase subunit c
T4277 22155-22161 GO_EXT:0016887 denotes ATPase
T4278 22191-22205 GO:0007569 denotes aging of cells
T4279 22200-22205 CL_GO_EXT:cell denotes cells
T4280 22277-22286 _FRAGMENT denotes endosomal
T4281 22297-22305 GO:0010008 denotes membrane
T4282 22306-22317 GO:0016197 denotes trafficking
T4283 22287-22305 GO:0005765 denotes lysosomal membrane
T4284 22287-22296 _FRAGMENT denotes lysosomal
T4285 22306-22317 GO:0007041 denotes trafficking
T4286 22297-22317 GO:0055085 denotes membrane trafficking
T4287 22330-22343 GO:0005739 denotes mitochondrial
T4288 22370-22379 PR_EXT:000022190 denotes subunit c
T4289 22531-22539 CL:0000540 denotes neuronal
T4290 22559-22570 PR_EXT:000006025 denotes cathepsin D
T4291 22571-22583 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T4292 22588-22598 SO_EXT:sequence_alteration_process denotes processing
T4293 22613-22615 UBERON:0002037 denotes Cb
T4294 22615-22619 PR_EXT:000005591 denotes Cln3
T4295 22626-22631 CL_GO_EXT:cell denotes cells
T4296 22636-22640 PR_EXT:000005591 denotes Cln3
T4297 22647-22651 NCBITaxon:10088 denotes mice
T4298 22676-22685 GO:0031982 denotes vesicular
T4299 22676-22697 GO:0016192 denotes vesicular trafficking
T4300 22705-22714 GO:0005764 denotes lysosomal
T4301 22744-22755 PR_EXT:000006025 denotes cathepsin D
T4302 22756-22766 SO_EXT:sequence_alteration_process denotes processing
T4303 22784-22788 PR_EXT:000005591 denotes CLN3
T4304 22793-22803 GO:0010467 denotes expression
T4305 22815-22820 CL_GO_EXT:cell denotes cells
T4306 22829-22838 GO:0005764 denotes lysosomal
T4307 22846-22857 PR_EXT:000006025 denotes cathepsin D
T4308 22858-22868 SO_EXT:sequence_alteration_process denotes processing
T4309 22879-22888 GO:0005764 denotes lysosomal
T4310 22889-22903 GO:0006885 denotes pH homeostasis
T4311 22956-22967 PR_EXT:000006023 denotes cathepsin B
T4312 22976-22980 PR_EXT:000016585 denotes CLN2
T4313 22981-22988 SO_EXT:sequence_coding_function denotes encoded
T4314 22989-22995 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T4315 22997-23001 PR_EXT:000016585 denotes TPPI
T4316 23012-23019 SO_EXT:sequence_alteration_process denotes altered
T4317 23063-23074 PR_EXT:000006025 denotes cathepsin D
T4318 23075-23082 CHEBI_PR_EXT:protein denotes protein
T4319 23083-23094 SO_EXT:sequence_alteration_entity_or_process denotes alterations
T4320 23127-23129 UBERON:0002037 denotes Cb
T4321 23129-23133 PR_EXT:000005591 denotes Cln3
T4322 23140-23145 CL_GO_EXT:cell denotes cells
T4323 23147-23158 PR_EXT:000006025 denotes cathepsin D
T4324 23159-23168 CHEBI_GO_SO_EXT:enzyme denotes enzymatic
T4325 23225-23236 PR_EXT:000006025 denotes cathepsin D
T4326 23273-23282 PR_EXT:000022190 denotes subunit c
T4327 23305-23313 _FRAGMENT denotes Aging of
T4328 23338-23343 GO:0007569 denotes cells
T4329 23325-23327 UBERON:0002037 denotes Cb
T4330 23327-23331 PR_EXT:000005591 denotes Cln3
T4331 23338-23343 CL_GO_EXT:cell denotes cells
T4332 23407-23416 PR_EXT:000022190 denotes subunit c
T4333 23417-23424 CHEBI_PR_EXT:protein denotes protein
T4334 23435-23453 GO:0043227 denotes membrane organelle
T4335 23474-23483 PR_EXT:000022190 denotes subunit c
T4336 23511-23513 UBERON:0002037 denotes Cb
T4337 23513-23517 PR_EXT:000005591 denotes Cln3
T4338 23524-23529 CL_GO_EXT:cell denotes cells
T4339 23563-23568 GO:0007568 denotes aging
T4340 23584-23593 GO:0005764 denotes Lysosomal
T4341 23598-23607 GO:0005768 denotes endosomal
T4342 23647-23659 GO:0005739 denotes mitochondria
T4343 23742-23744 UBERON:0002037 denotes Cb
T4344 23744-23748 PR_EXT:000005591 denotes Cln3
T4345 23795-23803 GO:0016020 denotes membrane
T4346 23795-23815 GO:0055085 denotes membrane trafficking
T4347 23853-23862 PR_EXT:000022190 denotes subunit c
T4348 23893-23901 GO:0005764 denotes lysosome
T4349 23964-23973 PR_EXT:000022190 denotes subunit c
T4350 23988-24001 GO:0005739 denotes Mitochondrial
T4351 24074-24080 NCBITaxon:33208 denotes animal
T4352 24137-24146 GO:0006914 denotes autophagy
T4353 24150-24159 GO:0005764 denotes lysosomal
T4354 24217-24225 PR_EXT:000005591 denotes battenin
T4355 24248-24261 GO:0005739 denotes mitochondrial
T4356 24303-24316 GO:0005739 denotes mitochondrial
T4357 24317-24327 GO_EXT:biogenesis_or_biosynthesis denotes biogenesis
T4358 24343-24352 _FRAGMENT denotes transport
T4359 24368-24370 _FRAGMENT denotes of
T4360 24385-24393 GO:0015031 denotes proteins
T4361 24357-24367 SO_EXT:sequence_alteration_process denotes processing
T4362 24357-24393 GO:0034982 denotes processing of mitochondrial proteins
T4363 24371-24384 GO:0005739 denotes mitochondrial
T4364 24385-24393 CHEBI_PR_EXT:protein denotes proteins
T4365 24398-24407 SO_EXT:wild_type_entity_or_quality denotes wild-type
T4366 24408-24410 UBERON:0002037 denotes Cb
T4367 24410-24414 PR_EXT:000005591 denotes Cln3
T4368 24415-24423 CL:0000540 denotes neuronal
T4369 24434-24439 CL_GO_EXT:cell denotes cells
T4370 24440-24448 PR_EXT:000005591 denotes battenin
T4371 24462-24471 GO_PATO_EXT:biological_localization_process_or_quality denotes localizes
T4372 24477-24482 _FRAGMENT denotes early
T4373 24492-24501 GO:0005769 denotes endosomes
T4374 24487-24501 GO:0005770 denotes late endosomes
T4375 24503-24511 PR_EXT:000005591 denotes Battenin
T4376 24541-24543 UBERON:0002037 denotes Cb
T4377 24543-24547 PR_EXT:000005591 denotes Cln3
T4378 24554-24562 CL:0000540 denotes neuronal
T4379 24617-24623 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T4380 24639-24648 GO_PATO_EXT:biological_localization_process_or_quality denotes localizes
T4381 24654-24663 GO:0005768 denotes endosomal
T4382 24664-24671 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T4383 24683-24689 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T4384 24690-24698 PR_EXT:000005591 denotes battenin
T4385 24699-24706 CHEBI_PR_EXT:protein denotes protein
T4386 24712-24722 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T4387 24723-24731 CHEBI_SO_EXT:epitope denotes epitopes
T4388 24735-24745 GO_EXT:biological_routing_or_transport denotes trafficked
T4389 24757-24766 SO_EXT:wild_type_entity_or_quality denotes wild-type
T4390 24767-24774 CHEBI_PR_EXT:protein denotes protein
T4391 24794-24798 PR_EXT:000005591 denotes CLN3
T4392 24799-24807 PR_EXT:000005591 denotes battenin
T5035 25473-25490 GO_EXT:neurotransmission denotes Neurotransmission
T5036 25509-25525 GO:0031988 denotes membrane vesicle
T5037 25518-25535 GO:0016192 denotes vesicle transport
T5038 25555-25565 GO:0008152 denotes metabolism
T5039 25588-25595 CL:0000540 denotes neurons
T5040 25611-25619 PR_EXT:000005591 denotes battenin
T5041 25649-25658 SO_EXT:wild_type_entity_or_quality denotes wild-type
T5042 25689-25691 UBERON:0002037 denotes Cb
T5043 25691-25695 PR_EXT:000005591 denotes Cln3
T5044 25702-25712 UBERON:0002037 denotes cerebellar
T5045 25713-25718 CL_GO_EXT:cell denotes cells
T5046 25770-25778 PR_EXT:000005591 denotes battenin
T5190 25821-25829 GO:0042571 denotes Antibody
T5191 25834-25838 CL_GO_EXT:cell denotes cell
T5192 25848-25856 CHEBI_EXT:33893 denotes reagents
T5193 25857-25863 PR_EXT:000011141 denotes Nestin
T5194 25865-25870 SO_EXT:sequence_cloned_entity denotes clone
T5195 25871-25874 NCBITaxon:10114 denotes Rat
T5196 25890-25896 PR_EXT:000002060 denotes Lamp 1
T5197 25898-25903 SO_EXT:sequence_cloned_entity denotes clone
T5198 25924-25930 PR_EXT:000002061 denotes Lamp 2
T5199 25932-25937 SO_EXT:sequence_cloned_entity denotes clone
T5200 25966-25976 GO:0042571 denotes antibodies
T5201 26090-26100 CHEBI_GO_EXT:biological_process_or_quality_or_role denotes Biological
T5202 26163-26172 PR_EXT:000022190 denotes subunit c
T5203 26173-26181 GO:0042571 denotes antibody
T5204 26278-26288 GO:0042571 denotes antibodies
T5205 26306-26310 PR_EXT:000007939 denotes GFAP
T5206 26339-26348 PR_EXT:000004967 denotes calbindin
T5207 26366-26370 PR_EXT:000006252 denotes NeuN
T5208 26393-26397 NCBITaxon:10633 denotes SV40
T5209 26400-26407 CHEBI_EXT:59132 denotes antigen
T5210 26455-26466 PR_EXT:000006025 denotes cathepsin D
T5211 26511-26523 CHEBI:18070 denotes cytochrome c
T5212 26511-26531 GO_EXT:0004129 denotes cytochrome c oxidase
T5213 26511-26542 PR_EXT:000005776 denotes cytochrome c oxidase subunit IV
T5214 26544-26548 PR_EXT:000005776 denotes cox4
T5215 26585-26588 GO_PR_EXT:protein_disulfide_isomerase denotes PDI
T5216 26634-26639 PR_EXT:000008131 denotes GM130
T5217 26653-26665 GO:0009293 denotes Transduction
T5218 26675-26682 PR_EXT:000028799 denotes tubulin
T5219 26702-26707 PR_EXT:000008813 denotes grp75
T5220 26728-26742 GO:0005769 denotes early endosome
T5221 26728-26752 PR_EXT:000006901 denotes early endosome antigen-1
T5222 26743-26750 CHEBI_EXT:59132 denotes antigen
T5223 26754-26758 PR_EXT:000006901 denotes EEA1
T5224 26797-26802 PR_EXT:000013639 denotes rab 7
T5225 26866-26876 GO:0042571 denotes antibodies
T5226 26940-26950 MOP:0000779 denotes conjugated
T5227 26961-26971 GO:0042571 denotes antibodies
T5228 27024-27028 CL_GO_EXT:cell denotes cell
T5229 27029-27036 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T5230 27058-27064 CHEBI:15956 denotes biotin
T5231 27106-27113 CHEBI:52071 denotes dextran
T5232 27114-27118 CHEBI:37926 denotes FITC
T5233 27132-27154 CHEBI:52117 denotes Lysotracker Red DND-99
T5234 27164-27173 CHEBI_EXT:polyatomic_entity_or_group denotes Molecular
T5235 27174-27180 CHEBI_SO_EXT:molecular_probe denotes Probes
T5658 27212-27227 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T5659 27231-27233 UBERON:0002037 denotes Cb
T5660 27233-27237 PR_EXT:000005591 denotes Cln3
T5661 27238-27248 UBERON:0002037 denotes cerebellar
T5662 27238-27257 CL:1001611 denotes cerebellar neuronal
T5663 27274-27278 PR_EXT:000005591 denotes Cln3
T5664 27294-27298 NCBITaxon:10088 denotes mice
T5665 27347-27351 NCBITaxon_UBERON_EXT:pup denotes pups
T5666 27466-27476 UBERON:0002037 denotes cerebellar
T5667 27466-27491 CL:0001031 denotes cerebellar granule neuron
T5668 27520-27525 GO:0007567 denotes natal
T5669 27537-27546 UBERON:0002037 denotes cerebella
T5670 27581-27594 CHEBI_EXT:saline_solution denotes Salt Solution
T5671 27634-27641 CHEBI:17234 denotes glucose
T5672 27643-27647 UBERON:0002415 denotes Tail
T5673 27681-27692 SO_EXT:genomic_DNA denotes genomic DNA
T5674 27689-27692 CHEBI_SO_EXT:DNA denotes DNA
T5675 27707-27716 SO_EXT:genotype_or_entity_with_genotype denotes genotypic
T5676 27727-27734 PR_EXT:000027795 denotes Trypsin
T5677 27735-27739 CHEBI_EXT:EDTA denotes EDTA
T5678 27762-27769 GO_PR_EXT:deoxyribonuclease_I denotes DNase I
T5679 27827-27836 UBERON:0002037 denotes cerebella
T5680 27881-27890 CHEBI_EXT:ornithine denotes ornithine
T5681 27954-27963 UBERON:0002037 denotes cerebella
T5682 28000-28003 CHEBI:16526 denotes CO2
T5683 28008-28022 CL:0000120 denotes granule neuron
T5684 28023-28029 GO_EXT:biological_growth_entity_or_process denotes growth
T5685 28110-28116 NCBITaxon:27592 denotes bovine
T5686 28117-28122 UBERON:0001977 denotes serum
T5687 28164-28167 CHEBI:32588 denotes KCl
T5688 28227-28237 NCBITaxon:11632 denotes retrovirus
T5689 28238-28249 GO:0009293 denotes transducing
T5690 28294-28301 CHEBI_EXT:59132 denotes antigen
T5691 28318-28326 CHEBI:7507 denotes neomycin
T5692 28338-28346 SO_EXT:0005853 denotes cassette
T5693 28454-28460 GO_EXT:biological_growth_entity_or_process denotes growth
T5694 28511-28517 GO_EXT:biological_growth_entity_or_process denotes growth
T5695 28549-28553 CHEBI:42768 denotes G418
T5696 28703-28709 SO_EXT:sequence_cloned_entity denotes clonal
T5697 28739-28747 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T5698 28808-28810 UBERON:0002037 denotes Cb
T5699 28810-28814 PR_EXT:000005591 denotes Cln3
T5700 28815-28819 CL_GO_EXT:cell denotes cell
T5701 28827-28829 UBERON:0002037 denotes Cb
T5702 28834-28838 CL_GO_EXT:cell denotes cell
T5703 28875-28884 CHEBI_EXT:ornithine denotes ornithine
T5704 28936-28939 CHEBI:16526 denotes CO2
T5705 29043-29051 CL:0000540 denotes Neuronal
T5706 29052-29067 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T5707 29120-29128 CHEBI:60004 denotes cocktail
T5708 29139-29144 PR_EXT:000007479 denotes α-FGF
T5709 29153-29157 CHEBI:48518 denotes IBMX
T5710 29166-29169 CHEBI:37537 denotes TPA
T5711 29177-29186 CHEBI:42471 denotes forskolin
T5712 29193-29201 CHEBI:18243 denotes dopamine
T6149 29227-29229 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T6150 29234-29245 SO_EXT:genomic_DNA denotes Genomic DNA
T6151 29242-29245 CHEBI_SO_EXT:DNA denotes DNA
T6152 29265-29269 UBERON:0002415 denotes tail
T6153 29283-29287 CL_GO_EXT:cell denotes cell
T6154 29332-29336 PR_EXT:000005591 denotes Cln3
T6155 29352-29358 SO_EXT:0001023 denotes allele
T6156 29383-29392 SO_EXT:wild_type_entity_or_quality denotes wild-type
T6157 29393-29400 SO_EXT:0000112 denotes primers
T6158 29490-29492 SO_EXT:0000028 denotes bp
T6159 29511-29518 SO_EXT:0000112 denotes primers
T6160 29612-29614 SO_EXT:0000028 denotes bp
T6161 29746-29749 CHEBI_SO_EXT:RNA denotes RNA
T6162 29764-29768 PR_EXT:000005591 denotes Cln3
T6163 29769-29771 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T6164 29776-29783 SO_EXT:0000112 denotes primers
T6348 29837-29846 PR_EXT:000022190 denotes Subunit c
T6349 29866-29871 CL_GO_EXT:cell denotes Cells
T6350 29944-29949 CL_GO_EXT:cell denotes cells
T6351 30038-30043 CL_GO_EXT:cell denotes cells
T6352 30057-30064 CHEBI_PR_EXT:protein denotes protein
T6353 30077-30082 CL_GO_EXT:cell denotes Cells
T6354 30219-30224 CL_GO_EXT:cell denotes cells
T6356 30267-30276 CHEBI_EXT:phosphate denotes phosphate
T6357 30277-30285 CHEBI_CHMO_EXT:buffer_process denotes buffered
T6358 30286-30292 CHEBI_EXT:saline_solution denotes saline
T6359 30358-30367 PR_EXT:000022190 denotes subunit c
T6360 30387-30391 CL_GO_EXT:cell denotes cell
T6361 30425-30432 CHEBI_PR_EXT:protein denotes protein
T6362 30460-30465 CL_GO_EXT:cell denotes cells
T6363 30487-30501 CHEBI:64276 denotes glutaraldehyde
T6364 30505-30521 CHEBI_EXT:paraformaldehye denotes paraformaldehyde
T6365 30531-30541 CHEBI:16223 denotes cacodylate
T6366 30542-30548 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T6367 30835-30843 GO:0042571 denotes antibody
T6368 30844-30849 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes label
T6642 32000-32006 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T6643 32108-32116 CHEBI_PR_EXT:protein denotes proteins
T6644 32136-32140 CL_GO_EXT:cell denotes cell
T6645 32183-32187 CHEBI:9754 denotes Tris
T6646 32200-32212 CHEBI:9750 denotes Triton X-100
T6647 32214-32222 GO:0016020 denotes membrane
T6648 32252-32271 CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor denotes protease inhibitors
T6649 32305-32316 CHEBI:7989 denotes pepstatin A
T6650 32345-32354 CHEBI:6426 denotes leupeptin
T6651 32533-32540 CHEBI_PR_EXT:protein denotes protein
T6652 32567-32574 CHEBI_PR_EXT:protein denotes Protein
T6653 32599-32602 CHEBI:8984 denotes SDS
T6654 32665-32669 CHEBI:9754 denotes tris
T6655 32670-32677 CHEBI:46760 denotes tricine
T6656 32678-32681 CHEBI:8984 denotes SDS
T6657 32706-32715 PR_EXT:000022190 denotes subunit c
T7103 32745-32756 PR_EXT:000006025 denotes Cathepsin D
T7104 32779-32785 UBERON:0000479 denotes tissue
T7105 32894-32901 CHEBI_PR_EXT:protein denotes protein
T7106 32937-32942 CL_GO_EXT:cell denotes cells
T7107 32954-32958 CHEBI:9754 denotes Tris
T7108 32973-32985 CHEBI:9750 denotes Triton X-100
T7109 33119-33126 CHEBI_PR_EXT:protein denotes protein
T7110 33177-33184 CHEBI_PR_EXT:protein denotes Protein
T7111 33210-33217 CHEBI_PR_EXT:protein denotes protein
T7112 33247-33258 PR_EXT:000006025 denotes cathepsin D
T7113 33278-33289 CHEBI_EXT:76219 denotes Fluorogenic
T7114 33300-33311 PR_EXT:000006025 denotes Cathepsin D
T7115 33481-33490 CHEBI_EXT:polyatomic_entity_or_group denotes Molecular
T7323 33687-33696 GO:0005764 denotes Lysosomal
T7324 33710-33726 GO:0006897 denotes endocytic uptake
T7325 33727-33732 CL_GO_EXT:cell denotes Cells
T7326 33771-33776 CL_GO_EXT:cell denotes cells
T7327 33815-33820 GO:0040007 denotes grown
T7328 33840-33846 GO_EXT:biological_growth_entity_or_process denotes Growth
T7329 33889-33895 GO_EXT:biological_growth_entity_or_process denotes growth
T7330 33943-33950 CHEBI:52071 denotes dextran
T7331 33951-33955 CHEBI:37926 denotes FITC
T7332 33961-33966 CL_GO_EXT:cell denotes cells
T7333 34044-34052 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeling
T7334 34054-34059 CL_GO_EXT:cell denotes cells
T7335 34129-34132 CHEBI_EXT:37958 denotes dye
T7336 34163-34175 CHEBI:16842 denotes formaldehyde
T7480 34370-34382 GO:0005739 denotes mitochondria
T7481 34526-34538 GO:0005739 denotes mitochondria
T7482 34596-34608 GO:0005739 denotes mitochondria
T7483 34872-34885 GO:0005739 denotes mitochondrial
T7611 35000-35003 CHEBI_EXT:ATP denotes ATP
T7612 35016-35019 CHEBI_EXT:ATP denotes ATP
T7613 35073-35077 CL_GO_EXT:cell denotes Cell
T7614 35161-35166 CL_GO_EXT:cell denotes cells
T7615 35333-35340 CHEBI_EXT:33893 denotes Reagent
T7616 35368-35372 CL_GO_EXT:cell denotes cell
T7617 35368-35378 GO:0019835 denotes cell lysis
T7618 35415-35418 CHEBI_EXT:ATP denotes ATP
T7772 35677-35694 CHEBI:16240 denotes Hydrogen peroxide
T7773 35711-35716 CL_GO_EXT:cell denotes Cells
T7774 35752-35757 CL_GO_EXT:cell denotes cells
T7775 35882-35899 CHEBI:16240 denotes hydrogen peroxide
T7776 35928-35933 CL_GO_EXT:cell denotes Cells
T7777 35968-35985 CHEBI:16240 denotes hydrogen peroxide
T7778 36084-36088 CL_GO_EXT:cell denotes Cell
T7779 36084-36102 GO:0008283 denotes Cell Proliferation
T7929 5427-5435 CL:0000540 denotes Neuronal
T7930 5436-5442 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T7931 5443-5453 GO:0010467 denotes expression
T7932 5457-5459 UBERON:0002037 denotes Cb
T7933 5459-5463 PR_EXT:000005591 denotes Cln3
T7934 5463-5464 SO_EXT:normal_or_wild_type_or_present denotes +
T7935 5465-5466 SO_EXT:normal_or_wild_type_or_present denotes +
T7936 5467-5472 CL_GO_EXT:cell denotes cells
T7937 5493-5495 UBERON:0002037 denotes Cb
T7938 5495-5499 PR_EXT:000005591 denotes Cln3
T7939 5499-5500 SO_EXT:normal_or_wild_type_or_present denotes +
T7940 5501-5502 SO_EXT:normal_or_wild_type_or_present denotes +
T7941 5503-5508 CL_GO_EXT:cell denotes cells
T7942 5536-5542 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T7943 5543-5553 GO:0042571 denotes antibodies
T7944 5564-5566 UBERON:0002037 denotes Cb
T7945 5566-5570 PR_EXT:000005591 denotes Cln3
T7946 5570-5571 SO_EXT:normal_or_wild_type_or_present denotes +
T7947 5572-5573 SO_EXT:normal_or_wild_type_or_present denotes +
T7948 5593-5599 PR_EXT:000011141 denotes nestin
T7949 5600-5610 GO:0010467 denotes expression
T7950 5623-5627 PR_EXT:000007939 denotes GFAP
T7951 5628-5638 GO:0010467 denotes expression
T7952 5662-5670 CL:0000540 denotes neuronal
T7953 5715-5730 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T7954 5731-5739 CHEBI:60004 denotes cocktail
T7955 5755-5757 UBERON:0002037 denotes Cb
T7956 5757-5761 PR_EXT:000005591 denotes Cln3
T7957 5761-5762 SO_EXT:normal_or_wild_type_or_present denotes +
T7958 5763-5764 SO_EXT:normal_or_wild_type_or_present denotes +
T7959 5765-5770 CL_GO_EXT:cell denotes cells
T7960 5780-5786 CL:0000540 denotes neuron
T7961 5817-5821 CL_GO_EXT:cell denotes cell
T7962 5817-5828 GO:0044297 denotes cell bodies
T7963 5846-5855 GO:0042995 denotes processes
T7964 5861-5865 PR_EXT:000010124 denotes MAP2
T7965 5874-5878 PR_EXT:000006252 denotes NeuN
T7966 5883-5893 GO:0010467 denotes expression
T7967 5909-5911 UBERON:0002037 denotes Cb
T7968 5911-5915 PR_EXT:000005591 denotes Cln3
T7969 5915-5916 SO_EXT:normal_or_wild_type_or_present denotes +
T7970 5917-5918 SO_EXT:normal_or_wild_type_or_present denotes +
T7971 5919-5924 CL_GO_EXT:cell denotes cells
T7972 5946-5961 CL:0000121 denotes Purkinje neuron
T7973 5962-5968 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T7974 5969-5978 PR_EXT:000004967 denotes calbindin
T7975 5984-5986 UBERON:0002037 denotes Cb
T7976 5986-5990 PR_EXT:000005591 denotes Cln3
T7977 5990-5991 SO_EXT:normal_or_wild_type_or_present denotes +
T7978 6003-6005 UBERON:0002037 denotes Cb
T7979 6005-6009 PR_EXT:000005591 denotes Cln3
T7980 6023-6027 CL_GO_EXT:cell denotes cell
T7981 6054-6060 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T8180 7812-7814 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T8181 7822-7826 PR_EXT:000005591 denotes Cln3
T8182 7827-7831 CHEBI_SO_EXT:mRNA denotes mRNA
T8183 7835-7844 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8184 7860-7862 UBERON:0002037 denotes Cb
T8185 7862-7866 PR_EXT:000005591 denotes Cln3
T8186 7873-7878 CL_GO_EXT:cell denotes cells
T8187 7879-7883 PR_EXT:000005591 denotes Cln3
T8188 7890-7897 SO:0001030 denotes forward
T8189 7899-7903 SO_EXT:0000147 denotes Exon
T8190 7907-7914 SO:0001031 denotes reverse
T8191 7915-7917 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T8192 7918-7930 SO_EXT:0000006 denotes PCR products
T8193 7953-7962 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8194 7964-7965 SO_EXT:normal_or_wild_type_or_present denotes +
T8195 7966-7967 SO_EXT:normal_or_wild_type_or_present denotes +
T8196 7983-7989 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8197 8006-8011 UBERON:0000955 denotes brain
T8198 8016-8020 CL_GO_EXT:cell denotes cell
T8199 8026-8029 CHEBI_SO_EXT:RNA denotes RNA
T8200 8031-8036 UBERON:0000955 denotes Brain
T8201 8041-8045 CL_GO_EXT:cell denotes cell
T8202 8051-8053 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T8203 8054-8075 SO_EXT:0000006 denotes PCR reaction products
T8204 8107-8123 CHEBI:4883 denotes ethidium-bromide
T8205 8132-8139 CHEBI:2511 denotes agarose
T8206 8146-8155 SO_EXT:wild_type_entity_or_quality denotes Wild-type
T8207 8156-8158 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T8208 8159-8170 SO_EXT:0000006 denotes PCR product
T8209 8190-8191 CHEBI_SO_EXT:base denotes b
T8210 8201-8207 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8211 8257-8258 CHEBI_SO_EXT:base denotes b
T8212 8282-8288 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8213 8289-8295 GO:0008380 denotes splice
T8214 8289-8304 SO_EXT:alternative_splice_variant denotes splice variants
T8400 8316-8324 PR_EXT:000005591 denotes Battenin
T8401 8329-8338 GO:0005764 denotes lysosomal
T8402 8343-8352 GO:0005768 denotes endosomal
T8403 8353-8359 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T8404 8375-8384 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8405 8400-8402 UBERON:0002037 denotes Cb
T8406 8402-8406 PR_EXT:000005591 denotes Cln3
T8407 8413-8423 UBERON:0002037 denotes cerebellar
T8408 8434-8439 CL_GO_EXT:cell denotes cells
T8409 8464-8473 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8410 8475-8477 UBERON:0002037 denotes Cb
T8411 8477-8481 PR_EXT:000005591 denotes Cln3
T8412 8481-8482 SO_EXT:normal_or_wild_type_or_present denotes +
T8413 8483-8484 SO_EXT:normal_or_wild_type_or_present denotes +
T8414 8501-8507 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8415 8509-8511 UBERON:0002037 denotes Cb
T8416 8511-8515 PR_EXT:000005591 denotes Cln3
T8417 8530-8540 UBERON:0002037 denotes cerebellar
T8418 8551-8556 CL_GO_EXT:cell denotes cells
T8419 8588-8597 GO:0005764 denotes lysosomes
T8420 8599-8605 PR_EXT:000002060 denotes Lamp 1
T8421 8608-8623 GO:0005769 denotes early endosomes
T8422 8625-8629 PR_EXT:000006901 denotes EEA1
T8423 8636-8650 GO:0005770 denotes late endosomes
T8424 8652-8656 PR_EXT:000013639 denotes Rab7
T8425 8706-8710 PR_EXT:000006901 denotes EEA1
T8426 8738-8742 PR_EXT:000013639 denotes Rab7
T8427 8875-8879 PR_EXT:000013639 denotes Rab7
T8428 8926-8932 PR_EXT:000002060 denotes Lamp 1
T8429 8993-8995 UBERON:0002037 denotes Cb
T8430 8995-8999 PR_EXT:000005591 denotes Cln3
T8431 9006-9011 CL_GO_EXT:cell denotes cells
T8432 9067-9071 PR_EXT:000006901 denotes EEA1
T8433 9076-9080 PR_EXT:000013639 denotes Rab7
T8434 9098-9104 PR_EXT:000002060 denotes Lamp 1
T8435 9178-9184 PR_EXT:000002060 denotes Lamp 1
T8436 9189-9193 PR_EXT:000006901 denotes EEA1
T8437 9194-9206 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T8438 9227-9231 PR_EXT:000013639 denotes Rab7
T8439 9283-9285 UBERON:0002037 denotes Cb
T8440 9285-9289 PR_EXT:000005591 denotes Cln3
T8441 9296-9301 CL_GO_EXT:cell denotes cells
T8442 9303-9312 SO_EXT:wild_type_entity_or_quality denotes Wild-type
T8443 9328-9330 UBERON:0002037 denotes Cb
T8444 9330-9334 PR_EXT:000005591 denotes Cln3
T8745 10430-10439 PR_EXT:000022190 denotes Subunit c
T8746 10467-10469 UBERON:0002037 denotes Cb
T8747 10469-10473 PR_EXT:000005591 denotes Cln3
T8748 10480-10490 UBERON:0002037 denotes cerebellar
T8749 10501-10506 CL_GO_EXT:cell denotes cells
T8750 10510-10519 PR_EXT:000022190 denotes Subunit c
T8751 10576-10580 GO_PATO_EXT:aging_or_agedness denotes aged
T8752 10581-10590 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8753 10606-10608 UBERON:0002037 denotes Cb
T8754 10608-10612 PR_EXT:000005591 denotes Cln3
T8755 10619-10624 CL_GO_EXT:cell denotes cells
T8756 10635-10644 SO_EXT:wild_type_entity_or_quality denotes Wild-type
T8757 10655-10657 UBERON:0002037 denotes Cb
T8758 10657-10661 PR_EXT:000005591 denotes Cln3
T8759 10661-10662 SO_EXT:normal_or_wild_type_or_present denotes +
T8760 10663-10664 SO_EXT:normal_or_wild_type_or_present denotes +
T8761 10684-10693 PR_EXT:000022190 denotes subunit c
T8762 10740-10742 UBERON:0002037 denotes Cb
T8763 10742-10746 PR_EXT:000005591 denotes Cln3
T8764 10760-10765 CL_GO_EXT:cell denotes cells
T8765 10785-10794 PR_EXT:000022190 denotes subunit c
T8766 10910-10919 PR_EXT:000022190 denotes subunit c
T8767 10996-11005 PR_EXT:000022190 denotes subunit c
T8768 11006-11013 CHEBI_PR_EXT:protein denotes protein
T8769 11079-11086 CHEBI_PR_EXT:protein denotes protein
T8770 11116-11125 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8771 11127-11128 SO_EXT:normal_or_wild_type_or_present denotes +
T8772 11129-11130 SO_EXT:normal_or_wild_type_or_present denotes +
T8773 11147-11153 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T8774 11219-11228 PR_EXT:000022190 denotes subunit c
T8775 11229-11236 CHEBI_PR_EXT:protein denotes protein
T8776 11240-11245 PR_EXT:000022190 denotes sub c
T8777 11289-11291 UBERON:0002037 denotes Cb
T8778 11291-11295 PR_EXT:000005591 denotes Cln3
T8779 11302-11307 CL_GO_EXT:cell denotes cells
T8780 11347-11356 PR_EXT:000022190 denotes subunit c
T8781 11357-11364 CHEBI_PR_EXT:protein denotes protein
T8782 11386-11395 SO_EXT:wild_type_entity_or_quality denotes wild-type
T8783 11405-11406 SO_EXT:normal_or_wild_type_or_present denotes +
T8784 11407-11408 SO_EXT:normal_or_wild_type_or_present denotes +
T8785 11411-11418 CHEBI_PR_EXT:protein denotes Protein
T8786 11445-11457 CHEBI:18070 denotes cytochrome c
T8787 11445-11465 GO_EXT:0004129 denotes cytochrome c oxidase
T8788 11445-11476 PR_EXT:000005776 denotes cytochrome c oxidase subunit IV
T8789 11480-11484 PR_EXT:000005776 denotes cox4
T8790 11506-11516 GO:0016234 denotes inclusions
T8791 11537-11541 GO_PATO_EXT:aging_or_agedness denotes aged
T8792 11553-11555 UBERON:0002037 denotes Cb
T8793 11555-11559 PR_EXT:000005591 denotes Cln3
T8794 11566-11571 CL_GO_EXT:cell denotes cells
T8795 11590-11603 GO:0005776 denotes autophagosome
T8796 11624-11632 GO:0016020 denotes membrane
T8797 11670-11682 GO:0005739 denotes mitochondria
T8798 11689-11697 CHEBI:10545 denotes electron
T8799 11689-11709 GO:0031045 denotes electron dense cores
T8800 11750-11759 GO:0031982 denotes vesicular
T8801 11780-11788 CHEBI:10545 denotes electron
T8802 11795-11804 GO:0016234 denotes inclusion
T8803 11858-11859 GO:0005739 denotes M
T8804 11861-11873 GO:0005739 denotes mitochondria
T9196 13625-13636 PR_EXT:000006025 denotes Cathepsin D
T9197 13637-13649 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T9198 13654-13664 SO_EXT:sequence_alteration_process denotes processing
T9199 13668-13677 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9200 13693-13695 UBERON:0002037 denotes Cb
T9201 13695-13699 PR_EXT:000005591 denotes Cln3
T9202 13706-13711 CL_GO_EXT:cell denotes cells
T9203 13733-13742 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9204 13758-13760 UBERON:0002037 denotes Cb
T9205 13760-13764 PR_EXT:000005591 denotes Cln3
T9206 13781-13786 CL_GO_EXT:cell denotes cells
T9207 13797-13808 PR_EXT:000006025 denotes cathepsin D
T9208 13809-13817 GO:0042571 denotes antibody
T9209 13833-13842 SO_EXT:sequence_alteration_process denotes processed
T9210 13847-13856 SO_EXT:sequence_alteration_process denotes processed
T9211 13866-13877 PR_EXT:000006025 denotes cathepsin D
T9212 13878-13885 CHEBI_PR_EXT:protein denotes protein
T9213 13896-13898 UBERON:0002037 denotes Cb
T9214 13898-13902 PR_EXT:000005591 denotes Cln3
T9215 13902-13903 SO_EXT:normal_or_wild_type_or_present denotes +
T9216 13904-13905 SO_EXT:normal_or_wild_type_or_present denotes +
T9217 13906-13911 CL_GO_EXT:cell denotes cells
T9218 13941-13948 GO:0005634 denotes nuclear
T9219 13953-13964 GO:0005737 denotes cytoplasmic
T9220 13982-13993 PR_EXT:000006025 denotes Cathepsin D
T9221 14015-14017 UBERON:0002037 denotes Cb
T9222 14017-14021 PR_EXT:000005591 denotes Cln3
T9223 14028-14033 CL_GO_EXT:cell denotes cells
T9224 14067-14074 GO:0005634 denotes nuclear
T9225 14086-14097 GO:0005737 denotes cytoplasmic
T9226 14127-14136 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9227 14137-14139 UBERON:0002037 denotes Cb
T9228 14139-14143 PR_EXT:000005591 denotes Cln3
T9229 14143-14144 SO_EXT:normal_or_wild_type_or_present denotes +
T9230 14145-14146 SO_EXT:normal_or_wild_type_or_present denotes +
T9231 14147-14152 CL_GO_EXT:cell denotes cells
T9232 14179-14190 PR_EXT:000006025 denotes Cathepsin D
T9233 14219-14228 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9234 14247-14251 PR_EXT:000005591 denotes Cln3
T9235 14267-14273 UBERON:0000479 denotes tissue
T9236 14277-14279 UBERON:0002037 denotes Cb
T9237 14279-14283 PR_EXT:000005591 denotes Cln3
T9238 14290-14298 CL_GO_EXT:cell denotes cellular
T9239 14331-14342 PR_EXT:000006025 denotes cathepsin D
T9240 14401-14410 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9241 14412-14414 SO_EXT:wild_type_entity_or_quality denotes wt
T9242 14416-14422 UBERON:0000479 denotes tissue
T9243 14427-14435 CL_GO_EXT:cell denotes cellular
T9244 14474-14480 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T9245 14555-14559 PR_EXT:000005591 denotes Cln3
T9246 14570-14572 UBERON:0002037 denotes Cb
T9247 14572-14576 PR_EXT:000005591 denotes Cln3
T9248 14583-14589 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T9249 14591-14592 SO_EXT:sequence_altered_entity_or_alteration_process denotes m
T9250 14664-14676 SO:0000985 denotes double-chain
T9251 14689-14695 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T9252 14721-14733 SO:0000984 denotes single-chain
T9253 14741-14747 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T9538 16967-16973 PR_EXT:000002061 denotes Lamp 2
T9539 16974-16982 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeling
T9540 16986-16995 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9541 17011-17013 UBERON:0002037 denotes Cb
T9542 17013-17017 PR_EXT:000005591 denotes Cln3
T9543 17024-17033 GO:0005764 denotes lysosomes
T9544 17034-17043 GO:0005764 denotes Lysosomal
T9545 17044-17052 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeling
T9546 17056-17065 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9547 17081-17083 UBERON:0002037 denotes Cb
T9548 17083-17087 PR_EXT:000005591 denotes Cln3
T9549 17104-17109 CL_GO_EXT:cell denotes cells
T9550 17131-17137 PR_EXT:000002061 denotes Lamp 2
T9551 17138-17146 GO:0042571 denotes antibody
T9552 17169-17172 CHEBI_EXT:37958 denotes dye
T9553 17186-17193 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T9554 17205-17212 GO:0005634 denotes nuclear
T9555 17223-17232 GO:0005764 denotes lysosomes
T9556 17247-17256 GO:0005764 denotes lysosomes
T9557 17264-17276 _FRAGMENT denotes periphery of
T9558 17287-17292 GO:0071944 denotes cells
T9559 17277-17286 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9560 17287-17292 CL_GO_EXT:cell denotes cells
T9561 17294-17296 UBERON:0002037 denotes Cb
T9562 17296-17300 PR_EXT:000005591 denotes Cln3
T9563 17300-17301 SO_EXT:normal_or_wild_type_or_present denotes +
T9564 17302-17303 SO_EXT:normal_or_wild_type_or_present denotes +
T9565 17363-17369 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T9566 17370-17375 CL_GO_EXT:cell denotes cells
T9567 17377-17379 UBERON:0002037 denotes Cb
T9568 17379-17383 PR_EXT:000005591 denotes Cln3
T9569 17412-17419 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T9570 17420-17428 GO:0031982 denotes vesicles
T9571 17451-17458 GO:0005634 denotes nuclear
T9572 17471-17477 PR_EXT:000002061 denotes Lamp 2
T9573 17560-17567 GO:0005634 denotes nuclear
T9574 17593-17595 UBERON:0002037 denotes Cb
T9575 17595-17599 PR_EXT:000005591 denotes Cln3
T9576 17606-17611 CL_GO_EXT:cell denotes cells
T9577 17696-17699 CHEBI_EXT:37958 denotes dye
T9578 17701-17710 SO_EXT:wild_type_entity_or_quality denotes Wild-type
T9579 17726-17728 UBERON:0002037 denotes Cb
T9580 17728-17732 PR_EXT:000005591 denotes Cln3
T9780 18429-18440 GO:0006897 denotes Endocytosis
T9781 18444-18453 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9782 18483-18485 UBERON:0002037 denotes Cb
T9783 18485-18489 PR_EXT:000005591 denotes Cln3
T9784 18496-18501 CL_GO_EXT:cell denotes cells
T9785 18502-18509 CHEBI:52071 denotes Dextran
T9786 18510-18514 CHEBI:37926 denotes FITC
T9787 18525-18534 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9788 18564-18566 UBERON:0002037 denotes Cb
T9789 18566-18570 PR_EXT:000005591 denotes Cln3
T9790 18587-18592 CL_GO_EXT:cell denotes cells
T9791 18606-18615 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9792 18617-18619 UBERON:0002037 denotes Cb
T9793 18619-18623 PR_EXT:000005591 denotes Cln3
T9794 18623-18624 SO_EXT:normal_or_wild_type_or_present denotes +
T9795 18625-18626 SO_EXT:normal_or_wild_type_or_present denotes +
T9796 18658-18660 UBERON:0002037 denotes Cb
T9797 18660-18664 PR_EXT:000005591 denotes Cln3
T9798 18664-18665 SO_EXT:normal_or_wild_type_or_present denotes +
T9799 18688-18693 CL_GO_EXT:cell denotes cells
T9800 18695-18702 CHEBI:52071 denotes dextran
T9801 18703-18707 CHEBI:37926 denotes FITC
T9802 18708-18713 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes label
T9803 18736-18743 GO:0005634 denotes nuclear
T9804 18754-18763 GO:0031982 denotes vesicular
T9805 18787-18794 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T9806 18795-18803 GO:0031982 denotes vesicles
T9807 18848-18855 CHEBI:52071 denotes dextran
T9808 18856-18860 CHEBI:37926 denotes FITC
T9809 18861-18866 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes label
T9810 18881-18887 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T9811 18889-18891 UBERON:0002037 denotes Cb
T9812 18891-18895 PR_EXT:000005591 denotes Cln3
T9813 18923-18928 CL_GO_EXT:cell denotes cells
T9814 18979-18987 GO:0031982 denotes vesicles
T9815 19002-19009 GO:0005634 denotes nuclear
T10038 20293-20306 GO:0005739 denotes Mitochondrial
T10039 20334-20343 SO_EXT:wild_type_entity_or_quality denotes wild-type
T10040 20373-20375 UBERON:0002037 denotes Cb
T10041 20375-20379 PR_EXT:000005591 denotes Cln3
T10042 20386-20391 CL_GO_EXT:cell denotes cells
T10043 20427-20436 SO_EXT:wild_type_entity_or_quality denotes wild-type
T10044 20452-20454 UBERON:0002037 denotes Cb
T10045 20454-20458 PR_EXT:000005591 denotes Cln3
T10046 20465-20478 GO:0005739 denotes mitochondrial
T10047 20525-20553 GO:0005743 denotes inner mitochondrial membrane
T10048 20554-20560 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T10049 20562-20567 PR_EXT:000008813 denotes grp75
T10050 20603-20615 GO:0005739 denotes mitochondria
T10051 20630-20636 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T10052 20637-20642 CL_GO_EXT:cell denotes cells
T10053 20644-20646 UBERON:0002037 denotes Cb
T10054 20646-20650 PR_EXT:000005591 denotes Cln3
T10055 20678-20687 SO_EXT:wild_type_entity_or_quality denotes wild-type
T10056 20688-20700 GO:0005739 denotes mitochondria
T10057 20702-20704 UBERON:0002037 denotes Cb
T10058 20704-20708 PR_EXT:000005591 denotes Cln3
T10059 20708-20709 SO_EXT:normal_or_wild_type_or_present denotes +
T10060 20710-20711 SO_EXT:normal_or_wild_type_or_present denotes +
T10061 20737-20750 GO:0005739 denotes Mitochondrial
T10062 20789-20798 SO_EXT:wild_type_entity_or_quality denotes wild-type
T10063 20829-20831 UBERON:0002037 denotes Cb
T10064 20831-20835 PR_EXT:000005591 denotes Cln3
T10065 20842-20854 GO:0005739 denotes mitochondria
T10066 20914-20922 CL_GO_EXT:cell denotes Cellular
T10067 20923-20926 CHEBI_EXT:ATP denotes ATP
T10068 20937-20946 SO_EXT:wild_type_entity_or_quality denotes wild-type
T10069 20976-20978 UBERON:0002037 denotes Cb
T10070 20978-20982 PR_EXT:000005591 denotes Cln3
T10071 20999-21004 CL_GO_EXT:cell denotes cells
T10072 21016-21025 SO_EXT:wild_type_entity_or_quality denotes Wild-type
T10073 21065-21067 UBERON:0002037 denotes Cb
T10074 21067-21071 PR_EXT:000005591 denotes Cln3
T10075 21078-21083 CL_GO_EXT:cell denotes cells
T10076 21101-21104 CHEBI_EXT:ATP denotes ATP
T10077 21123-21125 UBERON:0002037 denotes Cb
T10078 21125-21129 PR_EXT:000005591 denotes Cln3
T10079 21136-21141 CL_GO_EXT:cell denotes cells
T10080 21192-21195 CHEBI_EXT:ATP denotes ATP
T10081 21268-21277 SO_EXT:wild_type_entity_or_quality denotes Wild-type
T10082 21295-21297 UBERON:0002037 denotes Cb
T10083 21297-21301 PR_EXT:000005591 denotes Cln3
T10084 21308-21316 CL_GO_EXT:cell denotes cellular
T10085 21317-21320 CHEBI_EXT:ATP denotes ATP
T10086 21471-21475 CL_GO_EXT:cell denotes Cell
T10087 21503-21520 CHEBI:16240 denotes hydrogen peroxide
T10088 21552-21554 UBERON:0002037 denotes Cb
T10089 21554-21558 PR_EXT:000005591 denotes Cln3
T10090 21565-21570 CL_GO_EXT:cell denotes cells
T10091 21602-21611 MOP:0000568 denotes oxidative
T10092 21622-21639 CHEBI:16240 denotes hydrogen peroxide
T10093 21651-21660 SO_EXT:wild_type_entity_or_quality denotes Wild-type
T10094 21698-21700 UBERON:0002037 denotes Cb
T10095 21700-21704 PR_EXT:000005591 denotes Cln3
T10096 21711-21716 CL_GO_EXT:cell denotes cells
T10097 21762-21766 CHEBI:16240 denotes H2O2
T10098 21787-21789 UBERON:0002037 denotes Cb
T10099 21789-21793 PR_EXT:000005591 denotes Cln3
T10100 21800-21805 CL_GO_EXT:cell denotes cells
T10101 21852-21856 CHEBI:16240 denotes H2O2
T1751 9557-9566 GO:0016234 denotes inclusion
T1752 9557-9576 GO:0070841 denotes inclusion formation
T1753 9609-9614 CL_GO_EXT:cell denotes cells
T3211 15548-15556 GO:0016020 denotes membrane
T3212 15548-15568 GO:0055085 denotes membrane trafficking
T5028 25329-25337 GO:0016020 denotes membrane
T5029 25329-25349 GO:0055085 denotes membrane trafficking
T5030 25354-25367 GO:0005739 denotes mitochondrial
T5031 25401-25403 UBERON:0002037 denotes Cb
T5032 25403-25407 PR_EXT:000005591 denotes Cln3
T5033 25414-25419 CL_GO_EXT:cell denotes cells
T5034 25454-25462 CL:0000540 denotes neuronal
T6355 30251-30263 CHEBI:16842 denotes formaldehyde
T2444 12363-12374 PR_EXT:000006025 denotes cathepsin D
T2445 12375-12383 SO_EXT:0001060 denotes isoforms
T2446 12403-12412 SO_EXT:wild_type_entity_or_quality denotes wild-type
T2447 12428-12430 UBERON:0002037 denotes Cb
T2448 12430-12434 PR_EXT:000005591 denotes Cln3
T2449 12441-12446 CL_GO_EXT:cell denotes cells
T2450 12462-12469 GO:0005634 denotes nuclear
T2451 12483-12492 GO:0031982 denotes vesicular
T2452 12493-12504 PR_EXT:000006025 denotes cathepsin D
T2453 12539-12548 GO_EXT:biological_routing_or_transport denotes transport
T2454 12553-12563 SO_EXT:sequence_alteration_process denotes processing
T2455 12576-12593 GO_EXT:secretory_pathway denotes secretory pathway
T2456 12598-12606 GO_EXT:biological_routing_or_transport denotes delivery
T2457 12614-12622 GO:0005764 denotes lysosome
T2458 12657-12659 UBERON:0002037 denotes Cb
T2459 12659-12663 PR_EXT:000005591 denotes Cln3
T2460 12670-12675 CL_GO_EXT:cell denotes cells
T2461 12677-12688 PR_EXT:000006025 denotes cathepsin D
T2462 12711-12720 GO:0031982 denotes vesicular
T2463 12734-12741 GO:0005634 denotes nuclear
T2464 12760-12769 SO_EXT:wild_type_entity_or_quality denotes wild-type
T2465 12770-12775 CL_GO_EXT:cell denotes cells
T2466 12803-12805 UBERON:0002037 denotes Cb
T2467 12805-12809 PR_EXT:000005591 denotes Cln3
T2468 12816-12820 CL_GO_EXT:cell denotes cell
T2469 12825-12829 PR_EXT:000005591 denotes Cln3
T2470 12836-12842 UBERON:0000479 denotes tissue
T2471 12891-12902 PR_EXT:000006025 denotes cathepsin D
T2472 12903-12911 SO_EXT:0001060 denotes isoforms
T2473 12923-12934 PR_EXT:000006025 denotes Cathepsin D
T2474 12935-12943 SO_EXT:0001060 denotes isoforms
T2475 12968-12977 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T2476 13045-13057 SO:0000984 denotes single chain
T596 1691-1699 PATO_UBERON_EXT:juvenile_organism denotes Juvenile
T597 1700-1708 CL:0000540 denotes neuronal
T598 1785-1794 UBERON_EXT:childhood denotes childhood
T599 1878-1883 GO_EXT:biological_movement_or_translocation_process denotes motor
T600 1888-1897 GO:0050890 denotes cognitive
T601 1917-1922 GO:0016265 denotes death
T602 1940-1947 SO_EXT:0000704 denotes genetic
T603 1969-1980 GO_SO_EXT:chromosome denotes chromosomes
T604 2009-2010 CHEBI_SO_EXT:base denotes b
T605 2011-2022 SO_EXT:genomic_DNA denotes genomic DNA
T606 2019-2022 CHEBI_SO_EXT:DNA denotes DNA
T607 2023-2031 SO_EXT:sequence_deletion_entity_or_process denotes deletion
T608 2039-2043 PR_EXT:000005591 denotes CLN3
T609 2044-2048 SO_EXT:0000704 denotes gene
T610 2067-2072 SO_EXT:0000147 denotes exons
T611 2097-2105 SO_EXT:0000188 denotes intronic
T612 2106-2109 CHEBI_SO_EXT:DNA denotes DNA
T613 2139-2146 CHEBI_PR_EXT:protein denotes protein
T614 2247-2260 GO:0044754 denotes autolysosomes
T615 2282-2294 _FRAGMENT denotes subunit c of
T616 2299-2333 PR_EXT:000022190 denotes mitochondrial ATP synthase complex
T617 2299-2333 GO:0005753 denotes mitochondrial ATP synthase complex
T618 2313-2316 CHEBI_EXT:ATP denotes ATP
T1682 6469-6479 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T1683 6480-6488 CHEBI_SO_EXT:epitope denotes epitopes
T1684 6510-6519 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T1685 6533-6535 UBERON:0002037 denotes Cb
T1686 6535-6539 PR_EXT:000005591 denotes Cln3
T1687 6546-6551 CL_GO_EXT:cell denotes cells
T1688 6553-6555 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T1689 6569-6577 PR_EXT:000005591 denotes battenin
T1690 6639-6643 PR_EXT:000005591 denotes Cln3
T1691 6644-6648 CHEBI_SO_EXT:mRNA denotes mRNA
T1692 6649-6657 SO_EXT:0001060 denotes isoforms
T1693 6661-6670 SO_EXT:wild_type_entity_or_quality denotes wild-type
T1694 6686-6691 CL_GO_EXT:cell denotes cells
T1695 6732-6735 CHEBI_SO_EXT:RNA denotes RNA
T1696 6750-6759 SO_EXT:wild_type_entity_or_quality denotes wild-type
T1697 6774-6778 PR_EXT:000005591 denotes Cln3
T1698 6794-6799 UBERON:0000955 denotes brain
T1699 6867-6873 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T1700 6874-6882 PR_EXT:000005591 denotes battenin
T1701 6905-6907 UBERON:0002037 denotes Cb
T1745 9446-9452 GO_EXT:biological_growth_entity_or_process denotes growth
T1746 9473-9482 SO_EXT:wild_type_entity_or_quality denotes wild-type
T1747 9498-9500 UBERON:0002037 denotes Cb
T1748 9500-9504 PR_EXT:000005591 denotes Cln3
T1749 9511-9516 CL_GO_EXT:cell denotes cells
T1750 9547-9556 PR_EXT:000022190 denotes subunit c
T1754 9620-9624 GO_PATO_EXT:aging_or_agedness denotes aged
T1755 9677-9679 UBERON:0002037 denotes Cb
T1756 9679-9683 PR_EXT:000005591 denotes Cln3
T1757 9690-9698 CL_GO_EXT:cell denotes cellular
T1758 9699-9708 PR_EXT:000022190 denotes subunit c
T1759 9744-9753 SO_EXT:wild_type_entity_or_quality denotes wild-type
T1760 9873-9882 PR_EXT:000022190 denotes subunit c
T1761 10025-10029 GO_PATO_EXT:aging_or_agedness denotes aged
T1762 10041-10043 UBERON:0002037 denotes Cb
T1763 10043-10047 PR_EXT:000005591 denotes Cln3
T1764 10054-10059 CL_GO_EXT:cell denotes cells
T1765 10119-10127 CHEBI:10545 denotes electron
T1766 10134-10144 GO:0016234 denotes inclusions
T1767 10184-10198 GO:0005776 denotes autophagosomes
T1768 10214-10235 GO:0031045 denotes dense core structures
T1769 10250-10262 GO:0005739 denotes mitochondria
T1770 10281-10289 GO:0031982 denotes vesicles
T1771 10301-10317 GO:0016234 denotes Inclusion bodies
T1772 10322-10336 GO:0005776 denotes autophagosomes
T1773 10378-10382 GO_PATO_EXT:aging_or_agedness denotes aged
T1774 10383-10392 SO_EXT:wild_type_entity_or_quality denotes wild-type
T3204 15432-15434 UBERON:0002037 denotes Cb
T3205 15434-15438 PR_EXT:000005591 denotes Cln3
T3206 15445-15450 CL_GO_EXT:cell denotes cells
T3207 15465-15484 GO:0043227 denotes membrane organelles
T3208 15498-15507 GO_EXT:biological_routing_or_transport denotes transport
T3209 15512-15522 SO_EXT:sequence_alteration_process denotes processing
T3210 15526-15537 PR_EXT:000006025 denotes cathepsin D
T3268 17839-17840 SO_EXT:wild_type_entity_or_quality denotes t
T3269 17841-17842 CL_GO_EXT:cell denotes e
T3270 17848-17849 GO:0005634 denotes e
T3271 17854-17855 GO:0005634 denotes l
T3272 17869-17872 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes ker
T3273 17874-17878 PR_EXT:000006901 denotes EEA1
T3274 17924-17947 GO:0006907 denotes fluid-phase endocytosis
T3275 17979-17981 UBERON:0002037 denotes Cb
T3276 17981-17985 PR_EXT:000005591 denotes Cln3
T3277 17992-17997 CL_GO_EXT:cell denotes cells
T3278 18014-18021 CHEBI:52071 denotes dextran
T3279 18022-18026 CHEBI:37926 denotes FITC
T3280 18097-18104 CHEBI:52071 denotes dextran
T3281 18105-18109 CHEBI:37926 denotes FITC
T3282 18111-18120 SO_EXT:wild_type_entity_or_quality denotes wild-type
T3283 18138-18143 CL_GO_EXT:cell denotes cells
T3284 18178-18187 GO:0006897 denotes endocytic
T3285 18178-18196 GO:0030139 denotes endocytic vesicles
T3286 18228-18235 GO:0005634 denotes nuclear
T3287 18264-18266 UBERON:0002037 denotes Cb
T3288 18266-18270 PR_EXT:000005591 denotes Cln3
T3289 18277-18282 CL_GO_EXT:cell denotes cells
T3290 18320-18327 CHEBI:52071 denotes dextran
T3291 18328-18332 CHEBI:37926 denotes FITC
T3292 18340-18350 GO_PATO_EXT:biological_localization_process_or_quality denotes localizing
T3293 18362-18370 GO:0031982 denotes vesicles
T3294 18396-18405 GO:0005737 denotes cytoplasm
T3295 18413-18417 CL_GO_EXT:cell denotes cell
T3296 19108-19109 GO:0006897 denotes n
T3297 19110-19111 PR_EXT:000005591 denotes l
T3298 19111-19112 CL_GO_EXT:cell denotes l
T3299 19112-19113 SO_EXT:wild_type_entity_or_quality denotes y
T3300 19115-19116 UBERON:0002037 denotes b
T3301 19123-19124 CL_GO_EXT:cell denotes s
T3302 19124-19125 GO:0005634 denotes u
T3303 19125-19126 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes b
T3304 19127-19128 SO_EXT:sequence_altered_entity_or_alteration_process denotes n
T3305 19131-19132 CL_GO_EXT:cell denotes c
T3306 19143-19151 GO:0005739 denotes hondrial
T4393 24808-24815 CHEBI_PR_EXT:protein denotes protein
T4394 24816-24828 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T4395 24873-24887 GO:0005770 denotes late endosomes
T4396 24892-24901 GO:0005764 denotes lysosomes
T4397 24909-24917 CL:0000540 denotes neuronal
T4398 24918-24923 CL_GO_EXT:cell denotes cells
T4399 24936-24945 GO:0005764 denotes lysosomes
T4400 24968-24977 GO:0005768 denotes endosomes
T4401 24988-24995 CL:0000540 denotes neurons
T4402 25030-25038 PR_EXT:000005591 denotes battenin
T4403 25062-25071 GO:0031982 denotes vesicular
T4404 25102-25110 GO:0016020 denotes membrane
T4405 25102-25122 GO:0055085 denotes membrane trafficking
T4406 25156-25165 GO:0031982 denotes vesicular
T4407 25156-25175 GO:0016192 denotes vesicular transport
T4408 25156-25165 _FRAGMENT denotes vesicular
T4409 25183-25189 GO:0006906 denotes fusion
T4410 25191-25200 GO:0006897 denotes Endocytic
T4411 25205-25214 GO:0005764 denotes lysosomal
T4412 25215-25223 GO_EXT:biological_routing_or_transport denotes targeted
T4413 25244-25257 GO:0005739 denotes mitochondrial
T4414 25244-25267 GO:0000422 denotes mitochondrial autophagy
R2 T164 T163 _lexicallyChainedTo trafficking,intracellular
R265 T616 T615 _lexicallyChainedTo mitochondrial ATP synthase complex,subunit c of
R2374 T4281 T4280 _lexicallyChainedTo membrane,endosomal
R2375 T4282 T4280 _lexicallyChainedTo trafficking,endosomal
R2376 T4285 T4284 _lexicallyChainedTo trafficking,lysosomal
R2377 T4328 T4327 _lexicallyChainedTo cells,Aging of
R2378 T4359 T4358 _lexicallyChainedTo of,transport
R2379 T4360 T4359 _lexicallyChainedTo proteins,of
R2380 T4373 T4372 _lexicallyChainedTo endosomes,early
R2381 T4409 T4408 _lexicallyChainedTo fusion,vesicular
R6049 T9558 T9557 _lexicallyChainedTo cells,periphery of

2_test

Id Subject Object Predicate Lexical cue
15588329-3146319-12950135 1924-1925 3146319 denotes 1
15588329-7553855-12950136 2156-2157 7553855 denotes 2
15588329-1535180-12950137 2335-2336 1535180 denotes 3
15588329-1535179-12950137 2335-2336 1535179 denotes 3
15588329-3146325-12950137 2335-2336 3146325 denotes 3
15588329-12706816-12950138 2781-2782 12706816 denotes 6
15588329-14622109-12950139 2849-2850 14622109 denotes 7
15588329-11590129-12950139 2849-2850 11590129 denotes 7
15588329-14699076-12950139 2849-2850 14699076 denotes 7
15588329-10319861-12950140 3029-3031 10319861 denotes 10
15588329-14660799-12950141 3032-3034 14660799 denotes 11
15588329-12374761-12950142 3542-3544 12374761 denotes 12
15588329-12374761-12950143 6490-6492 12374761 denotes 12
15588329-12440525-12950144 12178-12180 12440525 denotes 13
15588329-12350228-12950145 13152-13154 12350228 denotes 14
15588329-10319861-12950146 16933-16935 10319861 denotes 10
15588329-11722572-12950147 16936-16938 11722572 denotes 15
15588329-12440525-12950148 19233-19235 12440525 denotes 13
15588329-12350228-12950149 22768-22770 12350228 denotes 14
15588329-7550114-12950150 22771-22773 7550114 denotes 16
15588329-10924275-12950151 22870-22872 10924275 denotes 17
15588329-10319861-12950152 22926-22928 10319861 denotes 10
15588329-11722572-12950153 22929-22931 11722572 denotes 15
15588329-3146318-12950154 23029-23031 3146318 denotes 18
15588329-11589013-12950154 23029-23031 11589013 denotes 18
15588329-10527801-12950154 23029-23031 10527801 denotes 18
15588329-7668331-12950155 24089-24091 7668331 denotes 21
15588329-10191128-12950155 24089-24091 10191128 denotes 21
15588329-11588986-12950155 24089-24091 11588986 denotes 21
15588329-11099404-12950156 24178-24180 11099404 denotes 24
15588329-14622109-12950157 24925-24926 14622109 denotes 7
15588329-11590129-12950158 24961-24962 11590129 denotes 8
15588329-14699076-12950159 24979-24980 14699076 denotes 9
15588329-15240864-12950160 24981-24983 15240864 denotes 25
15588329-12374761-12950161 26153-26155 12374761 denotes 12
15588329-12374761-12950162 27331-27333 12374761 denotes 12
15588329-8248201-12950163 27511-27513 8248201 denotes 26
15588329-3023876-12950164 28348-28350 3023876 denotes 27
15588329-9671979-12950165 28378-28380 9671979 denotes 28
15588329-11092756-12950166 29097-29099 11092756 denotes 29
15588329-12374761-12950167 29831-29833 12374761 denotes 12
15588329-12374761-12950168 30641-30643 12374761 denotes 12
15588329-12374761-12950169 32654-32656 12374761 denotes 12