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PMC:539061 / 60868-62281 JSONTXT

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craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T13242 11-17 NCBITaxon:39107 denotes murine
T13243 18-23 PR:000015308 denotes Snail
T13244 24-32 SO:0000167 denotes promoter
T13245 62-76 SO:0000121 denotes forward primer
T13246 159-173 SO:0000132 denotes reverse primer
T13247 248-253 NCBITaxon:10088 denotes mouse
T13248 254-261 SO:0001026 denotes genomic
T13249 285-296 SO:0000006 denotes PCR product
T13250 388-395 GO:0006266 denotes ligated
T13251 415-421 SO:0000440 denotes vector
T13252 477-485 SO:0000167 denotes promoter
T13253 582-588 SO:0000440 denotes vector
T13254 686-701 SO:0000409 denotes binding element
T13255 801-815 SO:0000121 denotes forward primer
T13256 877-891 SO:0000132 denotes reverse primer
T13257 967-972 PR:000015308 denotes Snail
T13258 981-994 GO:0097617 denotes hybridization
T13259 1022-1028 SO:0000205 denotes 3′ UTR
T13260 1046-1060 SO:0000121 denotes forward primer
T13261 1102-1116 SO:0000132 denotes reverse primer
T13262 1154-1161 SO:0001026 denotes genomic
T13263 1185-1196 SO:0000006 denotes PCR product
T13264 1218-1225 GO:0006266 denotes ligated
T13265 1245-1251 SO:0000440 denotes vector
T13266 1265-1271 SO:0000417 denotes domain
T13267 1275-1280 PR:000009231 denotes Ajuba
T13268 1381-1387 SO:0000440 denotes vector

2_test

Id Subject Object Predicate Lexical cue
15630473-10330178-84810337 1321-1322 10330178 denotes 9
T59433 1321-1322 10330178 denotes 9

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T13270 9-10 CHEBI_SO_EXT:base denotes b
T13271 11-17 NCBITaxon:39107 denotes murine
T13272 18-23 PR_EXT:000015308 denotes Snail
T13273 24-32 SO_EXT:0000167 denotes promoter
T13274 62-76 SO_EXT:0000121 denotes forward primer
T13275 97-105 SO_EXT:biological_sequence denotes sequence
T13276 159-173 SO_EXT:0000132 denotes reverse primer
T13277 194-202 SO_EXT:biological_sequence denotes sequence
T13278 248-253 NCBITaxon:10088 denotes mouse
T13279 254-265 SO_EXT:genomic_DNA denotes genomic DNA
T13280 262-265 CHEBI_SO_EXT:DNA denotes DNA
T13281 285-296 SO_EXT:0000006 denotes PCR product
T13282 388-395 GO:0006266 denotes ligated
T13283 415-421 SO_EXT:0000440 denotes vector
T13284 477-485 SO_EXT:0000167 denotes promoter
T13285 572-575 GO_EXT:0015925 denotes gal
T13286 582-588 SO_EXT:0000440 denotes vector
T13287 658-673 SO_EXT:point_mutation_entity_or_process denotes point mutations
T13288 681-685 GO_PR_EXT:SMAD denotes SMAD
T13289 686-693 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T13290 686-701 SO_EXT:0000409 denotes binding element
T13291 801-815 SO_EXT:0000121 denotes forward primer
T13292 877-891 SO_EXT:0000132 denotes reverse primer
T13293 952-958 CHEBI_SO_EXT:molecular_probe denotes probes
T13294 967-972 PR_EXT:000015308 denotes Snail
T13295 981-994 GO:0097617 denotes hybridization
T13296 1022-1028 SO_EXT:0000205 denotes 3′ UTR
T13297 1046-1060 SO_EXT:0000121 denotes forward primer
T13298 1102-1116 SO_EXT:0000132 denotes reverse primer
T13299 1154-1165 SO_EXT:genomic_DNA denotes genomic DNA
T13300 1162-1165 CHEBI_SO_EXT:DNA denotes DNA
T13301 1185-1196 SO_EXT:0000006 denotes PCR product
T13302 1218-1225 GO:0006266 denotes ligated
T13303 1245-1251 SO_EXT:0000440 denotes vector
T13304 1265-1271 SO_EXT:0000417 denotes domain
T13305 1275-1280 PR_EXT:000009231 denotes Ajuba
T13306 1333-1338 SO_EXT:sequence_fusion_process denotes fused
T13307 1381-1387 SO_EXT:0000440 denotes vector

craft-sa-dev

Id Subject Object Predicate Lexical cue
T13310 0-3 DT denotes The
T13311 24-32 NN denotes promoter
T13312 4-7 CD denotes 2.2
T13313 8-10 NN denotes kb
T13314 7-8 HYPH denotes -
T13315 11-17 JJ denotes murine
T13316 18-23 NN denotes Snail
T13317 37-46 VBN denotes generated
T13318 33-36 VBD denotes was
T13319 47-49 IN denotes by
T13320 50-53 NN denotes PCR
T13321 54-59 VBG denotes using
T13322 60-61 DT denotes a
T13323 70-76 NN denotes primer
T13324 62-69 JJ denotes forward
T13325 77-81 IN denotes with
T13326 82-84 DT denotes an
T13327 97-105 NN denotes sequence
T13328 85-89 NN denotes XbaI
T13329 90-96 NN denotes linker
T13330 105-107 , denotes ,
T13331 107-108 CD denotes 5
T13332 111-141 NN denotes TCTAGAATTGTTTGCTGCTGTATGGTCTTC
T13333 108-109 SYM denotes ′
T13334 109-110 HYPH denotes -
T13335 141-142 HYPH denotes -
T13336 142-143 CD denotes 3
T13337 143-144 SYM denotes ′
T13338 144-146 , denotes ,
T13339 146-151 IN denotes along
T13340 152-156 IN denotes with
T13341 157-158 DT denotes a
T13342 167-173 NN denotes primer
T13343 159-166 JJ denotes reverse
T13344 174-178 IN denotes with
T13345 179-180 DT denotes a
T13346 194-202 NN denotes sequence
T13347 181-186 NN denotes BglII
T13348 187-193 NN denotes linker
T13349 202-204 , denotes ,
T13350 204-205 CD denotes 5
T13351 235-239 NN denotes GTAG
T13352 205-206 SYM denotes ′
T13353 206-207 HYPH denotes -
T13354 208-233 NN denotes AGATCTGTTGGCCAGAGCGACCTAG
T13355 233-234 HYPH denotes -
T13356 239-240 HYPH denotes -
T13357 240-241 CD denotes 3
T13358 241-242 SYM denotes ′
T13359 242-244 , denotes ,
T13360 244-247 CC denotes and
T13361 248-253 NN denotes mouse
T13362 262-265 NN denotes DNA
T13363 254-261 JJ denotes genomic
T13364 266-268 IN denotes as
T13365 269-270 DT denotes a
T13366 271-279 NN denotes template
T13367 279-280 . denotes .
T13368 280-472 sentence denotes The PCR product was purified with the Gel Extraction Kit (Qiagen, Valencia, California, United States) and ligated into pCRII-TOPO TA vector (Invitrogen, Carlsbad, California, United States).
T13369 281-284 DT denotes The
T13370 289-296 NN denotes product
T13371 285-288 NN denotes PCR
T13372 301-309 VBN denotes purified
T13373 297-300 VBD denotes was
T13374 310-314 IN denotes with
T13375 315-318 DT denotes the
T13376 334-337 NN denotes Kit
T13377 319-322 NN denotes Gel
T13378 323-333 NN denotes Extraction
T13379 338-339 -LRB- denotes (
T13380 339-345 NNP denotes Qiagen
T13381 345-347 , denotes ,
T13382 347-355 NNP denotes Valencia
T13383 355-357 , denotes ,
T13384 357-367 NNP denotes California
T13385 367-369 , denotes ,
T13386 369-375 NNP denotes United
T13387 376-382 NNP denotes States
T13388 382-383 -RRB- denotes )
T13389 384-387 CC denotes and
T13390 388-395 VBN denotes ligated
T13391 396-400 IN denotes into
T13392 401-406 NN denotes pCRII
T13393 407-411 NN denotes TOPO
T13394 406-407 HYPH denotes -
T13395 415-421 NN denotes vector
T13396 412-414 NN denotes TA
T13397 422-423 -LRB- denotes (
T13398 423-433 NNP denotes Invitrogen
T13399 433-435 , denotes ,
T13400 435-443 NNP denotes Carlsbad
T13401 443-445 , denotes ,
T13402 445-455 NNP denotes California
T13403 455-457 , denotes ,
T13404 457-463 NNP denotes United
T13405 464-470 NNP denotes States
T13406 470-471 -RRB- denotes )
T13407 471-472 . denotes .
T13408 472-653 sentence denotes The promoter was verified by sequencing and digested with XbaI and BglII and subcloned into the pβ-gal BASIC vector (BD Biosciences Clontech, Palo Alto, California, United States).
T13409 473-476 DT denotes The
T13410 477-485 NN denotes promoter
T13411 490-498 VBN denotes verified
T13412 486-489 VBD denotes was
T13413 499-501 IN denotes by
T13414 502-512 NN denotes sequencing
T13415 513-516 CC denotes and
T13416 517-525 VBN denotes digested
T13417 526-530 IN denotes with
T13418 531-535 NN denotes XbaI
T13419 536-539 CC denotes and
T13420 540-545 NN denotes BglII
T13421 546-549 CC denotes and
T13422 550-559 VBN denotes subcloned
T13423 560-564 IN denotes into
T13424 565-568 DT denotes the
T13425 582-588 NN denotes vector
T13426 569-571 NN denotes pβ
T13427 572-575 NN denotes gal
T13428 571-572 HYPH denotes -
T13429 576-581 NN denotes BASIC
T13430 589-590 -LRB- denotes (
T13431 605-613 NNP denotes Clontech
T13432 590-592 NNP denotes BD
T13433 593-604 NNP denotes Biosciences
T13434 613-615 , denotes ,
T13435 615-619 NNP denotes Palo
T13436 620-624 NNP denotes Alto
T13437 624-626 , denotes ,
T13438 626-636 NNP denotes California
T13439 636-638 , denotes ,
T13440 638-644 NNP denotes United
T13441 645-651 NNP denotes States
T13442 651-652 -RRB- denotes )
T13443 652-653 . denotes .
T13444 653-947 sentence denotes The point mutations in the SMAD binding element was generated with the Quik-Change Kit (Stratagene, La Jolla, California, United States) using the forward primer 5′- GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC-3′ and the reverse primer 5′- GCTTTT- CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC-3′.
T13445 654-657 DT denotes The
T13446 664-673 NNS denotes mutations
T13447 658-663 NN denotes point
T13448 706-715 VBN denotes generated
T13449 674-676 IN denotes in
T13450 677-680 DT denotes the
T13451 694-701 NN denotes element
T13452 681-685 NN denotes SMAD
T13453 686-693 NN denotes binding
T13454 702-705 VBD denotes was
T13455 716-720 IN denotes with
T13456 721-724 DT denotes the
T13457 737-740 NNP denotes Kit
T13458 725-729 NNP denotes Quik
T13459 730-736 NNP denotes Change
T13460 729-730 HYPH denotes -
T13461 741-742 -LRB- denotes (
T13462 742-752 NNP denotes Stratagene
T13463 752-754 , denotes ,
T13464 754-756 NNP denotes La
T13465 757-762 NNP denotes Jolla
T13466 762-764 , denotes ,
T13467 764-774 NNP denotes California
T13468 774-776 , denotes ,
T13469 776-782 NNP denotes United
T13470 783-789 NNP denotes States
T13471 789-790 -RRB- denotes )
T13472 791-796 VBG denotes using
T13473 797-800 DT denotes the
T13474 809-815 NN denotes primer
T13475 801-808 JJ denotes forward
T13476 816-817 CD denotes 5
T13477 820-865 NN denotes GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
T13478 817-818 SYM denotes ′
T13479 818-819 HYPH denotes -
T13480 865-866 HYPH denotes -
T13481 866-867 CD denotes 3
T13482 867-868 SYM denotes ′
T13483 869-872 CC denotes and
T13484 873-876 DT denotes the
T13485 885-891 NN denotes primer
T13486 877-884 JJ denotes reverse
T13487 892-893 CD denotes 5
T13488 904-943 NN denotes CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
T13489 893-894 SYM denotes ′
T13490 894-895 HYPH denotes -
T13491 896-902 NN denotes GCTTTT
T13492 902-903 HYPH denotes -
T13493 943-944 HYPH denotes -
T13494 944-945 CD denotes 3
T13495 945-946 SYM denotes ′
T13496 946-947 . denotes .
T13497 947-1180 sentence denotes The probes for the Snail in situ hybridization were generated against the 3′ UTR by PCR using the forward primer 5′- ACCTTCTCCCGCATGTCCTTGCTCC-3′ and the reverse primer 5′- CTGCTGAGGCATGGTTACAGCTGG-3′, and genomic DNA as a template.
T13498 948-951 DT denotes The
T13499 952-958 NNS denotes probes
T13500 1000-1009 VBN denotes generated
T13501 959-962 IN denotes for
T13502 963-966 DT denotes the
T13503 981-994 NN denotes hybridization
T13504 967-972 NN denotes Snail
T13505 973-975 FW denotes in
T13506 976-980 FW denotes situ
T13507 995-999 VBD denotes were
T13508 1010-1017 IN denotes against
T13509 1018-1021 DT denotes the
T13510 1025-1028 NN denotes UTR
T13511 1022-1023 CD denotes 3
T13512 1023-1024 SYM denotes ′
T13513 1029-1031 IN denotes by
T13514 1032-1035 NN denotes PCR
T13515 1036-1041 VBG denotes using
T13516 1042-1045 DT denotes the
T13517 1054-1060 NN denotes primer
T13518 1046-1053 JJ denotes forward
T13519 1061-1062 CD denotes 5
T13520 1065-1090 NN denotes ACCTTCTCCCGCATGTCCTTGCTCC
T13521 1062-1063 SYM denotes ′
T13522 1063-1064 HYPH denotes -
T13523 1090-1091 HYPH denotes -
T13524 1091-1092 CD denotes 3
T13525 1092-1093 SYM denotes ′
T13526 1094-1097 CC denotes and
T13527 1098-1101 DT denotes the
T13528 1110-1116 NN denotes primer
T13529 1102-1109 JJ denotes reverse
T13530 1117-1118 CD denotes 5
T13531 1121-1145 NN denotes CTGCTGAGGCATGGTTACAGCTGG
T13532 1118-1119 SYM denotes ′
T13533 1119-1120 HYPH denotes -
T13534 1145-1146 HYPH denotes -
T13535 1146-1147 CD denotes 3
T13536 1147-1148 SYM denotes ′
T13537 1148-1150 , denotes ,
T13538 1150-1153 CC denotes and
T13539 1154-1161 JJ denotes genomic
T13540 1162-1165 NN denotes DNA
T13541 1166-1168 IN denotes as
T13542 1169-1170 DT denotes a
T13543 1171-1179 NN denotes template
T13544 1179-1180 . denotes .
T13545 1180-1252 sentence denotes The PCR product was gel purified and ligated into pCRII-TOPO TA vector.
T13546 1181-1184 DT denotes The
T13547 1189-1196 NN denotes product
T13548 1185-1188 NN denotes PCR
T13549 1205-1213 VBN denotes purified
T13550 1197-1200 VBD denotes was
T13551 1201-1204 NN denotes gel
T13552 1214-1217 CC denotes and
T13553 1218-1225 VBN denotes ligated
T13554 1226-1230 IN denotes into
T13555 1231-1236 NN denotes pCRII
T13556 1237-1241 NN denotes TOPO
T13557 1236-1237 HYPH denotes -
T13558 1245-1251 NN denotes vector
T13559 1242-1244 NN denotes TA
T13560 1251-1252 . denotes .
T13561 1252-1413 sentence denotes The pre-LIM domain of Ajuba was generated essentially as described [9], but was fused to GFP by subcloning from the pEGFP-N1 20 vector (BD Biosciences Clontech)
T13562 1253-1256 DT denotes The
T13563 1265-1271 NN denotes domain
T13564 1257-1264 JJ denotes pre-LIM
T13565 1285-1294 VBN denotes generated
T13566 1272-1274 IN denotes of
T13567 1275-1280 NN denotes Ajuba
T13568 1281-1284 VBD denotes was
T13569 1295-1306 RB denotes essentially
T13570 1307-1309 IN denotes as
T13571 1310-1319 VBN denotes described
T13572 1320-1321 -LRB- denotes [
T13573 1321-1322 CD denotes 9
T13574 1322-1323 -RRB- denotes ]
T13575 1323-1325 , denotes ,
T13576 1325-1328 CC denotes but
T13577 1329-1332 VBD denotes was
T13578 1333-1338 VBN denotes fused
T13579 1339-1341 IN denotes to
T13580 1342-1345 NN denotes GFP
T13581 1346-1348 IN denotes by
T13582 1349-1359 VBG denotes subcloning
T13583 1360-1364 IN denotes from
T13584 1365-1368 DT denotes the
T13585 1381-1387 NN denotes vector
T13586 1369-1374 NN denotes pEGFP
T13587 1375-1377 NN denotes N1
T13588 1374-1375 HYPH denotes -
T13589 1378-1380 CD denotes 20
T13590 1388-1389 -LRB- denotes (
T13591 1404-1412 NNP denotes Clontech
T13592 1389-1391 NNP denotes BD
T13593 1392-1403 NNP denotes Biosciences
T13594 1412-1413 -RRB- denotes )
R8600 T13310 T13311 det The,promoter
R8601 T13311 T13317 nsubjpass promoter,generated
R8602 T13312 T13313 nummod 2.2,kb
R8603 T13313 T13311 nmod kb,promoter
R8604 T13314 T13313 punct -,kb
R8605 T13315 T13311 amod murine,promoter
R8606 T13316 T13311 compound Snail,promoter
R8607 T13318 T13317 auxpass was,generated
R8608 T13319 T13317 prep by,generated
R8609 T13320 T13319 pobj PCR,by
R8610 T13321 T13317 advcl using,generated
R8611 T13322 T13323 det a,primer
R8612 T13323 T13321 dobj primer,using
R8613 T13324 T13323 amod forward,primer
R8614 T13325 T13321 prep with,using
R8615 T13326 T13327 det an,sequence
R8616 T13327 T13325 pobj sequence,with
R8617 T13328 T13327 compound XbaI,sequence
R8618 T13329 T13327 compound linker,sequence
R8619 T13330 T13327 punct ", ",sequence
R8620 T13331 T13332 nummod 5,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8621 T13332 T13327 appos TCTAGAATTGTTTGCTGCTGTATGGTCTTC,sequence
R8622 T13333 T13331 punct ′,5
R8623 T13334 T13332 punct -,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8624 T13335 T13332 punct -,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8625 T13336 T13332 nummod 3,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8626 T13337 T13332 punct ′,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8627 T13338 T13327 punct ", ",sequence
R8628 T13339 T13327 cc along,sequence
R8629 T13340 T13339 dep with,along
R8630 T13341 T13342 det a,primer
R8631 T13342 T13327 conj primer,sequence
R8632 T13343 T13342 amod reverse,primer
R8633 T13344 T13342 prep with,primer
R8634 T13345 T13346 det a,sequence
R8635 T13346 T13344 pobj sequence,with
R8636 T13347 T13346 compound BglII,sequence
R8637 T13348 T13346 compound linker,sequence
R8638 T13349 T13346 punct ", ",sequence
R8639 T13350 T13351 nummod 5,GTAG
R8640 T13351 T13346 appos GTAG,sequence
R8641 T13352 T13350 punct ′,5
R8642 T13353 T13351 punct -,GTAG
R8643 T13354 T13351 compound AGATCTGTTGGCCAGAGCGACCTAG,GTAG
R8644 T13355 T13351 punct -,GTAG
R8645 T13356 T13351 punct -,GTAG
R8646 T13357 T13351 nummod 3,GTAG
R8647 T13358 T13351 punct ′,GTAG
R8648 T13359 T13342 punct ", ",primer
R8649 T13360 T13342 cc and,primer
R8650 T13361 T13362 nmod mouse,DNA
R8651 T13362 T13342 conj DNA,primer
R8652 T13363 T13362 amod genomic,DNA
R8653 T13364 T13362 prep as,DNA
R8654 T13365 T13366 det a,template
R8655 T13366 T13364 pobj template,as
R8656 T13367 T13317 punct .,generated
R8657 T13369 T13370 det The,product
R8658 T13370 T13372 nsubjpass product,purified
R8659 T13371 T13370 compound PCR,product
R8660 T13373 T13372 auxpass was,purified
R8661 T13374 T13372 prep with,purified
R8662 T13375 T13376 det the,Kit
R8663 T13376 T13374 pobj Kit,with
R8664 T13377 T13378 compound Gel,Extraction
R8665 T13378 T13376 compound Extraction,Kit
R8666 T13379 T13380 punct (,Qiagen
R8667 T13380 T13376 parataxis Qiagen,Kit
R8668 T13381 T13380 punct ", ",Qiagen
R8669 T13382 T13380 npadvmod Valencia,Qiagen
R8670 T13383 T13380 punct ", ",Qiagen
R8671 T13384 T13380 npadvmod California,Qiagen
R8672 T13385 T13380 punct ", ",Qiagen
R8673 T13386 T13387 compound United,States
R8674 T13387 T13380 npadvmod States,Qiagen
R8675 T13388 T13380 punct ),Qiagen
R8676 T13389 T13372 cc and,purified
R8677 T13390 T13372 conj ligated,purified
R8678 T13391 T13390 prep into,ligated
R8679 T13392 T13393 compound pCRII,TOPO
R8680 T13393 T13395 compound TOPO,vector
R8681 T13394 T13393 punct -,TOPO
R8682 T13395 T13391 pobj vector,into
R8683 T13396 T13395 compound TA,vector
R8684 T13397 T13398 punct (,Invitrogen
R8685 T13398 T13395 parataxis Invitrogen,vector
R8686 T13399 T13398 punct ", ",Invitrogen
R8687 T13400 T13398 npadvmod Carlsbad,Invitrogen
R8688 T13401 T13398 punct ", ",Invitrogen
R8689 T13402 T13398 npadvmod California,Invitrogen
R8690 T13403 T13398 punct ", ",Invitrogen
R8691 T13404 T13405 compound United,States
R8692 T13405 T13398 npadvmod States,Invitrogen
R8693 T13406 T13398 punct ),Invitrogen
R8694 T13407 T13372 punct .,purified
R8695 T13409 T13410 det The,promoter
R8696 T13410 T13411 nsubjpass promoter,verified
R8697 T13412 T13411 auxpass was,verified
R8698 T13413 T13411 prep by,verified
R8699 T13414 T13413 pobj sequencing,by
R8700 T13415 T13411 cc and,verified
R8701 T13416 T13411 conj digested,verified
R8702 T13417 T13416 prep with,digested
R8703 T13418 T13417 pobj XbaI,with
R8704 T13419 T13418 cc and,XbaI
R8705 T13420 T13418 conj BglII,XbaI
R8706 T13421 T13416 cc and,digested
R8707 T13422 T13416 conj subcloned,digested
R8708 T13423 T13422 prep into,subcloned
R8709 T13424 T13425 det the,vector
R8710 T13425 T13423 pobj vector,into
R8711 T13426 T13427 compound pβ,gal
R8712 T13427 T13425 compound gal,vector
R8713 T13428 T13427 punct -,gal
R8714 T13429 T13425 compound BASIC,vector
R8715 T13430 T13431 punct (,Clontech
R8716 T13431 T13425 parataxis Clontech,vector
R8717 T13432 T13431 compound BD,Clontech
R8718 T13433 T13431 compound Biosciences,Clontech
R8719 T13434 T13431 punct ", ",Clontech
R8720 T13435 T13436 compound Palo,Alto
R8721 T13436 T13431 npadvmod Alto,Clontech
R8722 T13437 T13431 punct ", ",Clontech
R8723 T13438 T13431 npadvmod California,Clontech
R8724 T13439 T13431 punct ", ",Clontech
R8725 T13440 T13441 compound United,States
R8726 T13441 T13431 npadvmod States,Clontech
R8727 T13442 T13431 punct ),Clontech
R8728 T13443 T13411 punct .,verified
R8729 T13445 T13446 det The,mutations
R8730 T13446 T13448 nsubjpass mutations,generated
R8731 T13447 T13446 compound point,mutations
R8732 T13449 T13446 prep in,mutations
R8733 T13450 T13451 det the,element
R8734 T13451 T13449 pobj element,in
R8735 T13452 T13451 compound SMAD,element
R8736 T13453 T13451 compound binding,element
R8737 T13454 T13448 auxpass was,generated
R8738 T13455 T13448 prep with,generated
R8739 T13456 T13457 det the,Kit
R8740 T13457 T13455 pobj Kit,with
R8741 T13458 T13459 compound Quik,Change
R8742 T13459 T13457 compound Change,Kit
R8743 T13460 T13459 punct -,Change
R8744 T13461 T13462 punct (,Stratagene
R8745 T13462 T13457 parataxis Stratagene,Kit
R8746 T13463 T13462 punct ", ",Stratagene
R8747 T13464 T13465 compound La,Jolla
R8748 T13465 T13462 npadvmod Jolla,Stratagene
R8749 T13466 T13462 punct ", ",Stratagene
R8750 T13467 T13462 npadvmod California,Stratagene
R8751 T13468 T13462 punct ", ",Stratagene
R8752 T13469 T13470 compound United,States
R8753 T13470 T13462 npadvmod States,Stratagene
R8754 T13471 T13462 punct ),Stratagene
R8755 T13472 T13448 advcl using,generated
R8756 T13473 T13474 det the,primer
R8757 T13474 T13472 dobj primer,using
R8758 T13475 T13474 amod forward,primer
R8759 T13476 T13477 nummod 5,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8760 T13477 T13474 appos GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC,primer
R8761 T13478 T13476 punct ′,5
R8762 T13479 T13477 punct -,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8763 T13480 T13477 punct -,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8764 T13481 T13477 nummod 3,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8765 T13482 T13477 punct ′,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8766 T13483 T13474 cc and,primer
R8767 T13484 T13485 det the,primer
R8768 T13485 T13474 conj primer,primer
R8769 T13486 T13485 amod reverse,primer
R8770 T13487 T13488 nummod 5,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8771 T13488 T13485 appos CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC,primer
R8772 T13489 T13487 punct ′,5
R8773 T13490 T13488 punct -,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8774 T13491 T13488 compound GCTTTT,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8775 T13492 T13488 punct -,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8776 T13493 T13488 punct -,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8777 T13494 T13488 nummod 3,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8778 T13495 T13488 punct ′,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8779 T13496 T13448 punct .,generated
R8780 T13498 T13499 det The,probes
R8781 T13499 T13500 nsubjpass probes,generated
R8782 T13501 T13499 prep for,probes
R8783 T13502 T13503 det the,hybridization
R8784 T13503 T13501 pobj hybridization,for
R8785 T13504 T13503 nmod Snail,hybridization
R8786 T13505 T13506 advmod in,situ
R8787 T13506 T13503 amod situ,hybridization
R8788 T13507 T13500 auxpass were,generated
R8789 T13508 T13500 prep against,generated
R8790 T13509 T13510 det the,UTR
R8791 T13510 T13508 pobj UTR,against
R8792 T13511 T13510 nummod 3,UTR
R8793 T13512 T13511 punct ′,3
R8794 T13513 T13500 prep by,generated
R8795 T13514 T13513 pobj PCR,by
R8796 T13515 T13500 advcl using,generated
R8797 T13516 T13517 det the,primer
R8798 T13517 T13515 dobj primer,using
R8799 T13518 T13517 amod forward,primer
R8800 T13519 T13520 nummod 5,ACCTTCTCCCGCATGTCCTTGCTCC
R8801 T13520 T13517 appos ACCTTCTCCCGCATGTCCTTGCTCC,primer
R8802 T13521 T13519 punct ′,5
R8803 T13522 T13520 punct -,ACCTTCTCCCGCATGTCCTTGCTCC
R8804 T13523 T13520 punct -,ACCTTCTCCCGCATGTCCTTGCTCC
R8805 T13524 T13520 nummod 3,ACCTTCTCCCGCATGTCCTTGCTCC
R8806 T13525 T13520 punct ′,ACCTTCTCCCGCATGTCCTTGCTCC
R8807 T13526 T13517 cc and,primer
R8808 T13527 T13528 det the,primer
R8809 T13528 T13517 conj primer,primer
R8810 T13529 T13528 amod reverse,primer
R8811 T13530 T13531 nummod 5,CTGCTGAGGCATGGTTACAGCTGG
R8812 T13531 T13528 appos CTGCTGAGGCATGGTTACAGCTGG,primer
R8813 T13532 T13530 punct ′,5
R8814 T13533 T13531 punct -,CTGCTGAGGCATGGTTACAGCTGG
R8815 T13534 T13531 punct -,CTGCTGAGGCATGGTTACAGCTGG
R8816 T13535 T13531 nummod 3,CTGCTGAGGCATGGTTACAGCTGG
R8817 T13536 T13531 punct ′,CTGCTGAGGCATGGTTACAGCTGG
R8818 T13537 T13528 punct ", ",primer
R8819 T13538 T13528 cc and,primer
R8820 T13539 T13540 amod genomic,DNA
R8821 T13540 T13528 conj DNA,primer
R8822 T13541 T13540 prep as,DNA
R8823 T13542 T13543 det a,template
R8824 T13543 T13541 pobj template,as
R8825 T13544 T13500 punct .,generated
R8826 T13546 T13547 det The,product
R8827 T13547 T13549 nsubjpass product,purified
R8828 T13548 T13547 compound PCR,product
R8829 T13550 T13549 auxpass was,purified
R8830 T13551 T13549 dep gel,purified
R8831 T13552 T13549 cc and,purified
R8832 T13553 T13549 conj ligated,purified
R8833 T13554 T13553 prep into,ligated
R8834 T13555 T13556 compound pCRII,TOPO
R8835 T13556 T13558 compound TOPO,vector
R8836 T13557 T13556 punct -,TOPO
R8837 T13558 T13554 pobj vector,into
R8838 T13559 T13558 compound TA,vector
R8839 T13560 T13549 punct .,purified
R8840 T13562 T13563 det The,domain
R8841 T13563 T13565 nsubjpass domain,generated
R8842 T13564 T13563 amod pre-LIM,domain
R8843 T13566 T13563 prep of,domain
R8844 T13567 T13566 pobj Ajuba,of
R8845 T13568 T13565 auxpass was,generated
R8846 T13569 T13565 advmod essentially,generated
R8847 T13570 T13571 mark as,described
R8848 T13571 T13565 advcl described,generated
R8849 T13572 T13573 punct [,9
R8850 T13573 T13571 parataxis 9,described
R8851 T13574 T13573 punct ],9
R8852 T13575 T13565 punct ", ",generated
R8853 T13576 T13565 cc but,generated
R8854 T13577 T13578 auxpass was,fused
R8855 T13578 T13565 conj fused,generated
R8856 T13579 T13578 prep to,fused
R8857 T13580 T13579 pobj GFP,to
R8858 T13581 T13578 prep by,fused
R8859 T13582 T13581 pcomp subcloning,by
R8860 T13583 T13582 prep from,subcloning
R8861 T13584 T13585 det the,vector
R8862 T13585 T13583 pobj vector,from
R8863 T13586 T13587 nmod pEGFP,N1
R8864 T13587 T13585 nmod N1,vector
R8865 T13588 T13587 punct -,N1
R8866 T13589 T13585 nummod 20,vector
R8867 T13590 T13591 punct (,Clontech
R8868 T13591 T13585 parataxis Clontech,vector
R8869 T13592 T13591 compound BD,Clontech
R8870 T13593 T13591 compound Biosciences,Clontech
R8871 T13594 T13591 punct ),Clontech