PMC:539061 / 39641-40523 JSONTXT

{"project":"craft-ca-core-dev","denotations":[{"id":"T6902","span":{"begin":16,"end":22},"obj":"PR:000000183"},{"id":"T6903","span":{"begin":34,"end":39},"obj":"PR:000015308"},{"id":"T6904","span":{"begin":40,"end":48},"obj":"SO:0000167"},{"id":"T6905","span":{"begin":172,"end":177},"obj":"PR:000015308"},{"id":"T6906","span":{"begin":178,"end":186},"obj":"SO:0000167"},{"id":"T6907","span":{"begin":254,"end":267},"obj":"CL:0000312"},{"id":"T6908","span":{"begin":285,"end":290},"obj":"PR:000015308"},{"id":"T6909","span":{"begin":291,"end":299},"obj":"SO:0000167"},{"id":"T6910","span":{"begin":347,"end":352},"obj":"PR:000015308"},{"id":"T6911","span":{"begin":433,"end":439},"obj":"PR:000000183"},{"id":"T6912","span":{"begin":502,"end":508},"obj":"PR:000000183"},{"id":"T6913","span":{"begin":551,"end":566},"obj":"SO:0000409"},{"id":"T6914","span":{"begin":600,"end":605},"obj":"PR:000015308"},{"id":"T6915","span":{"begin":606,"end":630},"obj":"SO:0000315"},{"id":"T6916","span":{"begin":692,"end":705},"obj":"CL:0000312"},{"id":"T6917","span":{"begin":707,"end":713},"obj":"PR:000000183"},{"id":"T6918","span":{"begin":782,"end":787},"obj":"PR:000015308"},{"id":"T6919","span":{"begin":788,"end":792},"obj":"SO:0000704"},{"id":"T6920","span":{"begin":818,"end":823},"obj":"PR:000015308"},{"id":"T6921","span":{"begin":864,"end":872},"obj":"SO:0000167"}],"text":"Within 0.5 h of TGF-β2 treatment, Snail promoter activity had increased 3-fold, and by 2 h, it peaked to approximately 10-fold over control levels (Figure 5D). Thereafter, Snail promoter activity rapidly returned to the basal levels seen in unstimulated keratinocytes. The kinetics of Snail promoter activity closely paralleled those observed for Snail protein induction. Moreover, the stimulatory effects appeared to be specific to TGF-β2, since they were abrogated either by heat inactivation of the TGF-β2 protein or by mutation of a putative SMAD binding element located about 1.8 kb 5′ from the Snail transcription start site (Figure 5D). Taken together, these results suggested that in keratinocytes, TGF-β2 signaling results in a pSMAD2-dependent transient activation of the Snail gene, and that maintenance of Snail protein relies, in part, upon sustained promoter activity."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T7168","span":{"begin":16,"end":22},"obj":"PR_EXT:000000183"},{"id":"T7169","span":{"begin":34,"end":39},"obj":"PR_EXT:000015308"},{"id":"T7170","span":{"begin":40,"end":48},"obj":"SO_EXT:0000167"},{"id":"T7171","span":{"begin":172,"end":177},"obj":"PR_EXT:000015308"},{"id":"T7172","span":{"begin":178,"end":186},"obj":"SO_EXT:0000167"},{"id":"T7173","span":{"begin":254,"end":267},"obj":"CL:0000312"},{"id":"T7174","span":{"begin":285,"end":290},"obj":"PR_EXT:000015308"},{"id":"T7175","span":{"begin":291,"end":299},"obj":"SO_EXT:0000167"},{"id":"T7176","span":{"begin":347,"end":352},"obj":"PR_EXT:000015308"},{"id":"T7177","span":{"begin":353,"end":360},"obj":"CHEBI_PR_EXT:protein"},{"id":"T7178","span":{"begin":433,"end":439},"obj":"PR_EXT:000000183"},{"id":"T7179","span":{"begin":502,"end":508},"obj":"PR_EXT:000000183"},{"id":"T7180","span":{"begin":509,"end":516},"obj":"CHEBI_PR_EXT:protein"},{"id":"T7181","span":{"begin":523,"end":531},"obj":"SO_EXT:sequence_alteration_entity_or_process"},{"id":"T7182","span":{"begin":546,"end":550},"obj":"GO_PR_EXT:SMAD"},{"id":"T7183","span":{"begin":551,"end":558},"obj":"CHEMINF_GO_EXT:chemical_binding_or_bond_formation"},{"id":"T7184","span":{"begin":551,"end":566},"obj":"SO_EXT:0000409"},{"id":"T7185","span":{"begin":586,"end":587},"obj":"CHEBI_SO_EXT:base"},{"id":"T7186","span":{"begin":588,"end":590},"obj":"SO_EXT:sequence_upstreamness"},{"id":"T7187","span":{"begin":600,"end":605},"obj":"PR_EXT:000015308"},{"id":"T7188","span":{"begin":606,"end":619},"obj":"GO_EXT:transcription"},{"id":"T7189","span":{"begin":606,"end":630},"obj":"SO_EXT:0000315"},{"id":"T7190","span":{"begin":692,"end":705},"obj":"CL:0000312"},{"id":"T7191","span":{"begin":707,"end":713},"obj":"PR_EXT:000000183"},{"id":"T7192","span":{"begin":714,"end":723},"obj":"GO_EXT:cell_communication_or_signaling_or_signal_transduction"},{"id":"T7193","span":{"begin":782,"end":787},"obj":"PR_EXT:000015308"},{"id":"T7194","span":{"begin":788,"end":792},"obj":"SO_EXT:0000704"},{"id":"T7195","span":{"begin":818,"end":823},"obj":"PR_EXT:000015308"},{"id":"T7196","span":{"begin":824,"end":831},"obj":"CHEBI_PR_EXT:protein"},{"id":"T7197","span":{"begin":864,"end":872},"obj":"SO_EXT:0000167"}],"text":"Within 0.5 h of TGF-β2 treatment, Snail promoter activity had increased 3-fold, and by 2 h, it peaked to approximately 10-fold over control levels (Figure 5D). Thereafter, Snail promoter activity rapidly returned to the basal levels seen in unstimulated keratinocytes. The kinetics of Snail promoter activity closely paralleled those observed for Snail protein induction. Moreover, the stimulatory effects appeared to be specific to TGF-β2, since they were abrogated either by heat inactivation of the TGF-β2 protein or by mutation of a putative SMAD binding element located about 1.8 kb 5′ from the Snail transcription start site (Figure 5D). Taken together, these results suggested that in keratinocytes, TGF-β2 signaling results in a pSMAD2-dependent transient activation of the Snail gene, and that maintenance of Snail protein relies, in part, upon sustained promoter activity."}

{"project":"craft-sa-dev","denotations":[{"id":"T8080","span":{"begin":0,"end":6},"obj":"IN"},{"id":"T8081","span":{"begin":62,"end":71},"obj":"VBN"},{"id":"T8082","span":{"begin":7,"end":10},"obj":"CD"},{"id":"T8083","span":{"begin":11,"end":12},"obj":"NN"},{"id":"T8084","span":{"begin":13,"end":15},"obj":"IN"},{"id":"T8085","span":{"begin":16,"end":19},"obj":"NN"},{"id":"T8086","span":{"begin":20,"end":22},"obj":"NN"},{"id":"T8087","span":{"begin":19,"end":20},"obj":"HYPH"},{"id":"T8088","span":{"begin":23,"end":32},"obj":"NN"},{"id":"T8089","span":{"begin":32,"end":34},"obj":","},{"id":"T8090","span":{"begin":34,"end":39},"obj":"NN"},{"id":"T8091","span":{"begin":40,"end":48},"obj":"NN"},{"id":"T8092","span":{"begin":49,"end":57},"obj":"NN"},{"id":"T8093","span":{"begin":58,"end":61},"obj":"VBD"},{"id":"T8094","span":{"begin":72,"end":78},"obj":"RB"},{"id":"T8095","span":{"begin":78,"end":80},"obj":","},{"id":"T8096","span":{"begin":80,"end":83},"obj":"CC"},{"id":"T8097","span":{"begin":84,"end":86},"obj":"IN"},{"id":"T8098","span":{"begin":95,"end":101},"obj":"VBD"},{"id":"T8099","span":{"begin":87,"end":88},"obj":"CD"},{"id":"T8100","span":{"begin":89,"end":90},"obj":"NN"},{"id":"T8101","span":{"begin":90,"end":92},"obj":","},{"id":"T8102","span":{"begin":92,"end":94},"obj":"PRP"},{"id":"T8103","span":{"begin":102,"end":104},"obj":"IN"},{"id":"T8104","span":{"begin":105,"end":118},"obj":"RB"},{"id":"T8105","span":{"begin":119,"end":126},"obj":"RB"},{"id":"T8106","span":{"begin":127,"end":131},"obj":"IN"},{"id":"T8107","span":{"begin":132,"end":139},"obj":"NN"},{"id":"T8108","span":{"begin":140,"end":146},"obj":"NNS"},{"id":"T8109","span":{"begin":147,"end":148},"obj":"-LRB-"},{"id":"T8110","span":{"begin":155,"end":157},"obj":"NN"},{"id":"T8111","span":{"begin":148,"end":154},"obj":"NN"},{"id":"T8112","span":{"begin":157,"end":158},"obj":"-RRB-"},{"id":"T8113","span":{"begin":158,"end":159},"obj":"."},{"id":"T8114","span":{"begin":159,"end":268},"obj":"sentence"},{"id":"T8115","span":{"begin":160,"end":170},"obj":"RB"},{"id":"T8116","span":{"begin":204,"end":212},"obj":"VBD"},{"id":"T8117","span":{"begin":170,"end":172},"obj":","},{"id":"T8118","span":{"begin":172,"end":177},"obj":"NN"},{"id":"T8119","span":{"begin":178,"end":186},"obj":"NN"},{"id":"T8120","span":{"begin":187,"end":195},"obj":"NN"},{"id":"T8121","span":{"begin":196,"end":203},"obj":"RB"},{"id":"T8122","span":{"begin":213,"end":215},"obj":"IN"},{"id":"T8123","span":{"begin":216,"end":219},"obj":"DT"},{"id":"T8124","span":{"begin":226,"end":232},"obj":"NNS"},{"id":"T8125","span":{"begin":220,"end":225},"obj":"JJ"},{"id":"T8126","span":{"begin":233,"end":237},"obj":"VBN"},{"id":"T8127","span":{"begin":238,"end":240},"obj":"IN"},{"id":"T8128","span":{"begin":241,"end":253},"obj":"JJ"},{"id":"T8129","span":{"begin":254,"end":267},"obj":"NNS"},{"id":"T8130","span":{"begin":267,"end":268},"obj":"."},{"id":"T8131","span":{"begin":268,"end":371},"obj":"sentence"},{"id":"T8132","span":{"begin":269,"end":272},"obj":"DT"},{"id":"T8133","span":{"begin":273,"end":281},"obj":"NNS"},{"id":"T8134","span":{"begin":317,"end":327},"obj":"VBD"},{"id":"T8135","span":{"begin":282,"end":284},"obj":"IN"},{"id":"T8136","span":{"begin":285,"end":290},"obj":"NN"},{"id":"T8137","span":{"begin":291,"end":299},"obj":"NN"},{"id":"T8138","span":{"begin":300,"end":308},"obj":"NN"},{"id":"T8139","span":{"begin":309,"end":316},"obj":"RB"},{"id":"T8140","span":{"begin":328,"end":333},"obj":"DT"},{"id":"T8141","span":{"begin":334,"end":342},"obj":"VBN"},{"id":"T8142","span":{"begin":343,"end":346},"obj":"IN"},{"id":"T8143","span":{"begin":347,"end":352},"obj":"NN"},{"id":"T8144","span":{"begin":353,"end":360},"obj":"NN"},{"id":"T8145","span":{"begin":361,"end":370},"obj":"NN"},{"id":"T8146","span":{"begin":370,"end":371},"obj":"."},{"id":"T8147","span":{"begin":371,"end":643},"obj":"sentence"},{"id":"T8148","span":{"begin":372,"end":380},"obj":"RB"},{"id":"T8149","span":{"begin":406,"end":414},"obj":"VBD"},{"id":"T8150","span":{"begin":380,"end":382},"obj":","},{"id":"T8151","span":{"begin":382,"end":385},"obj":"DT"},{"id":"T8152","span":{"begin":398,"end":405},"obj":"NNS"},{"id":"T8153","span":{"begin":386,"end":397},"obj":"JJ"},{"id":"T8154","span":{"begin":415,"end":417},"obj":"TO"},{"id":"T8155","span":{"begin":418,"end":420},"obj":"VB"},{"id":"T8156","span":{"begin":421,"end":429},"obj":"JJ"},{"id":"T8157","span":{"begin":430,"end":432},"obj":"IN"},{"id":"T8158","span":{"begin":433,"end":436},"obj":"NN"},{"id":"T8159","span":{"begin":437,"end":439},"obj":"NN"},{"id":"T8160","span":{"begin":436,"end":437},"obj":"HYPH"},{"id":"T8161","span":{"begin":439,"end":441},"obj":","},{"id":"T8162","span":{"begin":441,"end":446},"obj":"IN"},{"id":"T8163","span":{"begin":457,"end":466},"obj":"VBN"},{"id":"T8164","span":{"begin":447,"end":451},"obj":"PRP"},{"id":"T8165","span":{"begin":452,"end":456},"obj":"VBD"},{"id":"T8166","span":{"begin":467,"end":473},"obj":"CC"},{"id":"T8167","span":{"begin":474,"end":476},"obj":"IN"},{"id":"T8168","span":{"begin":477,"end":481},"obj":"NN"},{"id":"T8169","span":{"begin":482,"end":494},"obj":"NN"},{"id":"T8170","span":{"begin":495,"end":497},"obj":"IN"},{"id":"T8171","span":{"begin":498,"end":501},"obj":"DT"},{"id":"T8172","span":{"begin":509,"end":516},"obj":"NN"},{"id":"T8173","span":{"begin":502,"end":505},"obj":"NN"},{"id":"T8174","span":{"begin":506,"end":508},"obj":"NN"},{"id":"T8175","span":{"begin":505,"end":506},"obj":"HYPH"},{"id":"T8176","span":{"begin":517,"end":519},"obj":"CC"},{"id":"T8177","span":{"begin":520,"end":522},"obj":"IN"},{"id":"T8178","span":{"begin":523,"end":531},"obj":"NN"},{"id":"T8179","span":{"begin":532,"end":534},"obj":"IN"},{"id":"T8180","span":{"begin":535,"end":536},"obj":"DT"},{"id":"T8181","span":{"begin":559,"end":566},"obj":"NN"},{"id":"T8182","span":{"begin":537,"end":545},"obj":"JJ"},{"id":"T8183","span":{"begin":546,"end":550},"obj":"NN"},{"id":"T8184","span":{"begin":551,"end":558},"obj":"NN"},{"id":"T8185","span":{"begin":567,"end":574},"obj":"VBN"},{"id":"T8186","span":{"begin":575,"end":580},"obj":"IN"},{"id":"T8187","span":{"begin":581,"end":584},"obj":"CD"},{"id":"T8188","span":{"begin":585,"end":587},"obj":"NN"},{"id":"T8189","span":{"begin":588,"end":589},"obj":"CD"},{"id":"T8190","span":{"begin":589,"end":590},"obj":"SYM"},{"id":"T8191","span":{"begin":591,"end":595},"obj":"IN"},{"id":"T8192","span":{"begin":596,"end":599},"obj":"DT"},{"id":"T8193","span":{"begin":626,"end":630},"obj":"NN"},{"id":"T8194","span":{"begin":600,"end":605},"obj":"NN"},{"id":"T8195","span":{"begin":606,"end":619},"obj":"NN"},{"id":"T8196","span":{"begin":620,"end":625},"obj":"NN"},{"id":"T8197","span":{"begin":631,"end":632},"obj":"-LRB-"},{"id":"T8198","span":{"begin":639,"end":641},"obj":"NN"},{"id":"T8199","span":{"begin":632,"end":638},"obj":"NN"},{"id":"T8200","span":{"begin":641,"end":642},"obj":"-RRB-"},{"id":"T8201","span":{"begin":642,"end":643},"obj":"."},{"id":"T8202","span":{"begin":643,"end":882},"obj":"sentence"},{"id":"T8203","span":{"begin":644,"end":649},"obj":"VBN"},{"id":"T8204","span":{"begin":674,"end":683},"obj":"VBD"},{"id":"T8205","span":{"begin":650,"end":658},"obj":"RB"},{"id":"T8206","span":{"begin":658,"end":660},"obj":","},{"id":"T8207","span":{"begin":660,"end":665},"obj":"DT"},{"id":"T8208","span":{"begin":666,"end":673},"obj":"NNS"},{"id":"T8209","span":{"begin":684,"end":688},"obj":"IN"},{"id":"T8210","span":{"begin":724,"end":731},"obj":"VBZ"},{"id":"T8211","span":{"begin":689,"end":691},"obj":"IN"},{"id":"T8212","span":{"begin":692,"end":705},"obj":"NNS"},{"id":"T8213","span":{"begin":705,"end":707},"obj":","},{"id":"T8214","span":{"begin":707,"end":710},"obj":"NN"},{"id":"T8215","span":{"begin":711,"end":713},"obj":"NN"},{"id":"T8216","span":{"begin":710,"end":711},"obj":"HYPH"},{"id":"T8217","span":{"begin":714,"end":723},"obj":"NN"},{"id":"T8218","span":{"begin":732,"end":734},"obj":"IN"},{"id":"T8219","span":{"begin":735,"end":736},"obj":"DT"},{"id":"T8220","span":{"begin":764,"end":774},"obj":"NN"},{"id":"T8221","span":{"begin":737,"end":743},"obj":"NN"},{"id":"T8222","span":{"begin":744,"end":753},"obj":"JJ"},{"id":"T8223","span":{"begin":743,"end":744},"obj":"HYPH"},{"id":"T8224","span":{"begin":754,"end":763},"obj":"JJ"},{"id":"T8225","span":{"begin":775,"end":777},"obj":"IN"},{"id":"T8226","span":{"begin":778,"end":781},"obj":"DT"},{"id":"T8227","span":{"begin":788,"end":792},"obj":"NN"},{"id":"T8228","span":{"begin":782,"end":787},"obj":"NN"},{"id":"T8229","span":{"begin":792,"end":794},"obj":","},{"id":"T8230","span":{"begin":794,"end":797},"obj":"CC"},{"id":"T8231","span":{"begin":798,"end":802},"obj":"IN"},{"id":"T8232","span":{"begin":832,"end":838},"obj":"VBZ"},{"id":"T8233","span":{"begin":803,"end":814},"obj":"NN"},{"id":"T8234","span":{"begin":815,"end":817},"obj":"IN"},{"id":"T8235","span":{"begin":818,"end":823},"obj":"NN"},{"id":"T8236","span":{"begin":824,"end":831},"obj":"NN"},{"id":"T8237","span":{"begin":838,"end":840},"obj":","},{"id":"T8238","span":{"begin":840,"end":842},"obj":"IN"},{"id":"T8239","span":{"begin":843,"end":847},"obj":"NN"},{"id":"T8240","span":{"begin":847,"end":849},"obj":","},{"id":"T8241","span":{"begin":849,"end":853},"obj":"IN"},{"id":"T8242","span":{"begin":854,"end":863},"obj":"JJ"},{"id":"T8243","span":{"begin":873,"end":881},"obj":"NN"},{"id":"T8244","span":{"begin":864,"end":872},"obj":"NN"},{"id":"T8245","span":{"begin":881,"end":882},"obj":"."}],"relations":[{"id":"R4854","pred":"prep","subj":"T8080","obj":"T8081"},{"id":"R4855","pred":"nummod","subj":"T8082","obj":"T8083"},{"id":"R4856","pred":"pobj","subj":"T8083","obj":"T8080"},{"id":"R4857","pred":"prep","subj":"T8084","obj":"T8083"},{"id":"R4858","pred":"compound","subj":"T8085","obj":"T8086"},{"id":"R4859","pred":"compound","subj":"T8086","obj":"T8088"},{"id":"R4860","pred":"punct","subj":"T8087","obj":"T8086"},{"id":"R4861","pred":"pobj","subj":"T8088","obj":"T8084"},{"id":"R4862","pred":"punct","subj":"T8089","obj":"T8081"},{"id":"R4863","pred":"compound","subj":"T8090","obj":"T8091"},{"id":"R4864","pred":"compound","subj":"T8091","obj":"T8092"},{"id":"R4865","pred":"nsubj","subj":"T8092","obj":"T8081"},{"id":"R4866","pred":"aux","subj":"T8093","obj":"T8081"},{"id":"R4867","pred":"advmod","subj":"T8094","obj":"T8081"},{"id":"R4868","pred":"punct","subj":"T8095","obj":"T8081"},{"id":"R4869","pred":"cc","subj":"T8096","obj":"T8081"},{"id":"R4870","pred":"prep","subj":"T8097","obj":"T8098"},{"id":"R4871","pred":"conj","subj":"T8098","obj":"T8081"},{"id":"R4872","pred":"nummod","subj":"T8099","obj":"T8100"},{"id":"R4873","pred":"pobj","subj":"T8100","obj":"T8097"},{"id":"R4874","pred":"punct","subj":"T8101","obj":"T8098"},{"id":"R4875","pred":"nsubj","subj":"T8102","obj":"T8098"},{"id":"R4876","pred":"prep","subj":"T8103","obj":"T8098"},{"id":"R4877","pred":"advmod","subj":"T8104","obj":"T8105"},{"id":"R4878","pred":"pcomp","subj":"T8105","obj":"T8103"},{"id":"R4879","pred":"prep","subj":"T8106","obj":"T8105"},{"id":"R4880","pred":"compound","subj":"T8107","obj":"T8108"},{"id":"R4881","pred":"pobj","subj":"T8108","obj":"T8106"},{"id":"R4882","pred":"punct","subj":"T8109","obj":"T8110"},{"id":"R4883","pred":"parataxis","subj":"T8110","obj":"T8098"},{"id":"R4884","pred":"compound","subj":"T8111","obj":"T8110"},{"id":"R4885","pred":"punct","subj":"T8112","obj":"T8110"},{"id":"R4886","pred":"punct","subj":"T8113","obj":"T8098"},{"id":"R4887","pred":"advmod","subj":"T8115","obj":"T8116"},{"id":"R4888","pred":"punct","subj":"T8117","obj":"T8116"},{"id":"R4889","pred":"compound","subj":"T8118","obj":"T8119"},{"id":"R4890","pred":"compound","subj":"T8119","obj":"T8120"},{"id":"R4891","pred":"nsubj","subj":"T8120","obj":"T8116"},{"id":"R4892","pred":"advmod","subj":"T8121","obj":"T8116"},{"id":"R4893","pred":"prep","subj":"T8122","obj":"T8116"},{"id":"R4894","pred":"det","subj":"T8123","obj":"T8124"},{"id":"R4895","pred":"pobj","subj":"T8124","obj":"T8122"},{"id":"R4896","pred":"amod","subj":"T8125","obj":"T8124"},{"id":"R4897","pred":"acl","subj":"T8126","obj":"T8124"},{"id":"R4898","pred":"prep","subj":"T8127","obj":"T8126"},{"id":"R4899","pred":"amod","subj":"T8128","obj":"T8129"},{"id":"R4900","pred":"pobj","subj":"T8129","obj":"T8127"},{"id":"R4901","pred":"punct","subj":"T8130","obj":"T8116"},{"id":"R4902","pred":"det","subj":"T8132","obj":"T8133"},{"id":"R4903","pred":"nsubj","subj":"T8133","obj":"T8134"},{"id":"R4904","pred":"prep","subj":"T8135","obj":"T8133"},{"id":"R4905","pred":"compound","subj":"T8136","obj":"T8137"},{"id":"R4906","pred":"compound","subj":"T8137","obj":"T8138"},{"id":"R4907","pred":"pobj","subj":"T8138","obj":"T8135"},{"id":"R4908","pred":"advmod","subj":"T8139","obj":"T8134"},{"id":"R4909","pred":"dobj","subj":"T8140","obj":"T8134"},{"id":"R4910","pred":"acl","subj":"T8141","obj":"T8140"},{"id":"R4911","pred":"prep","subj":"T8142","obj":"T8141"},{"id":"R4912","pred":"compound","subj":"T8143","obj":"T8144"},{"id":"R4913","pred":"compound","subj":"T8144","obj":"T8145"},{"id":"R4914","pred":"pobj","subj":"T8145","obj":"T8142"},{"id":"R4915","pred":"punct","subj":"T8146","obj":"T8134"},{"id":"R4916","pred":"advmod","subj":"T8148","obj":"T8149"},{"id":"R4917","pred":"punct","subj":"T8150","obj":"T8149"},{"id":"R4918","pred":"det","subj":"T8151","obj":"T8152"},{"id":"R4919","pred":"nsubj","subj":"T8152","obj":"T8149"},{"id":"R4920","pred":"amod","subj":"T8153","obj":"T8152"},{"id":"R4921","pred":"aux","subj":"T8154","obj":"T8155"},{"id":"R4922","pred":"xcomp","subj":"T8155","obj":"T8149"},{"id":"R4923","pred":"acomp","subj":"T8156","obj":"T8155"},{"id":"R4924","pred":"prep","subj":"T8157","obj":"T8156"},{"id":"R4925","pred":"compound","subj":"T8158","obj":"T8159"},{"id":"R4926","pred":"pobj","subj":"T8159","obj":"T8157"},{"id":"R4927","pred":"punct","subj":"T8160","obj":"T8159"},{"id":"R4928","pred":"punct","subj":"T8161","obj":"T8155"},{"id":"R4929","pred":"mark","subj":"T8162","obj":"T8163"},{"id":"R4930","pred":"advcl","subj":"T8163","obj":"T8155"},{"id":"R4931","pred":"nsubjpass","subj":"T8164","obj":"T8163"},{"id":"R4932","pred":"auxpass","subj":"T8165","obj":"T8163"},{"id":"R4933","pred":"preconj","subj":"T8166","obj":"T8167"},{"id":"R4934","pred":"prep","subj":"T8167","obj":"T8163"},{"id":"R4935","pred":"compound","subj":"T8168","obj":"T8169"},{"id":"R4936","pred":"pobj","subj":"T8169","obj":"T8167"},{"id":"R4937","pred":"prep","subj":"T8170","obj":"T8169"},{"id":"R4938","pred":"det","subj":"T8171","obj":"T8172"},{"id":"R4939","pred":"pobj","subj":"T8172","obj":"T8170"},{"id":"R4940","pred":"compound","subj":"T8173","obj":"T8174"},{"id":"R4941","pred":"compound","subj":"T8174","obj":"T8172"},{"id":"R4942","pred":"punct","subj":"T8175","obj":"T8174"},{"id":"R4943","pred":"cc","subj":"T8176","obj":"T8167"},{"id":"R4944","pred":"conj","subj":"T8177","obj":"T8167"},{"id":"R4945","pred":"pobj","subj":"T8178","obj":"T8177"},{"id":"R4946","pred":"prep","subj":"T8179","obj":"T8178"},{"id":"R4947","pred":"det","subj":"T8180","obj":"T8181"},{"id":"R4948","pred":"pobj","subj":"T8181","obj":"T8179"},{"id":"R4949","pred":"amod","subj":"T8182","obj":"T8181"},{"id":"R4950","pred":"compound","subj":"T8183","obj":"T8184"},{"id":"R4951","pred":"compound","subj":"T8184","obj":"T8181"},{"id":"R4952","pred":"acl","subj":"T8185","obj":"T8181"},{"id":"R4953","pred":"quantmod","subj":"T8186","obj":"T8187"},{"id":"R4954","pred":"nummod","subj":"T8187","obj":"T8188"},{"id":"R4955","pred":"npadvmod","subj":"T8188","obj":"T8185"},{"id":"R4956","pred":"nummod","subj":"T8189","obj":"T8188"},{"id":"R4957","pred":"punct","subj":"T8190","obj":"T8188"},{"id":"R4958","pred":"prep","subj":"T8191","obj":"T8188"},{"id":"R4959","pred":"det","subj":"T8192","obj":"T8193"},{"id":"R4960","pred":"pobj","subj":"T8193","obj":"T8191"},{"id":"R4961","pred":"compound","subj":"T8194","obj":"T8193"},{"id":"R4962","pred":"compound","subj":"T8195","obj":"T8193"},{"id":"R4963","pred":"compound","subj":"T8196","obj":"T8193"},{"id":"R4964","pred":"punct","subj":"T8197","obj":"T8198"},{"id":"R4965","pred":"parataxis","subj":"T8198","obj":"T8163"},{"id":"R4966","pred":"compound","subj":"T8199","obj":"T8198"},{"id":"R4967","pred":"punct","subj":"T8200","obj":"T8198"},{"id":"R4968","pred":"punct","subj":"T8201","obj":"T8149"},{"id":"R4969","pred":"advcl","subj":"T8203","obj":"T8204"},{"id":"R4970","pred":"advmod","subj":"T8205","obj":"T8203"},{"id":"R4971","pred":"punct","subj":"T8206","obj":"T8204"},{"id":"R4972","pred":"det","subj":"T8207","obj":"T8208"},{"id":"R4973","pred":"nsubj","subj":"T8208","obj":"T8204"},{"id":"R4974","pred":"mark","subj":"T8209","obj":"T8210"},{"id":"R4975","pred":"advcl","subj":"T8210","obj":"T8204"},{"id":"R4976","pred":"prep","subj":"T8211","obj":"T8210"},{"id":"R4977","pred":"pobj","subj":"T8212","obj":"T8211"},{"id":"R4978","pred":"punct","subj":"T8213","obj":"T8210"},{"id":"R4979","pred":"compound","subj":"T8214","obj":"T8215"},{"id":"R4980","pred":"compound","subj":"T8215","obj":"T8217"},{"id":"R4981","pred":"punct","subj":"T8216","obj":"T8215"},{"id":"R4982","pred":"nsubj","subj":"T8217","obj":"T8210"},{"id":"R4983","pred":"prep","subj":"T8218","obj":"T8210"},{"id":"R4984","pred":"det","subj":"T8219","obj":"T8220"},{"id":"R4985","pred":"pobj","subj":"T8220","obj":"T8218"},{"id":"R4986","pred":"npadvmod","subj":"T8221","obj":"T8222"},{"id":"R4987","pred":"amod","subj":"T8222","obj":"T8220"},{"id":"R4988","pred":"punct","subj":"T8223","obj":"T8222"},{"id":"R4989","pred":"amod","subj":"T8224","obj":"T8220"},{"id":"R4990","pred":"prep","subj":"T8225","obj":"T8220"},{"id":"R4991","pred":"det","subj":"T8226","obj":"T8227"},{"id":"R4992","pred":"pobj","subj":"T8227","obj":"T8225"},{"id":"R4993","pred":"compound","subj":"T8228","obj":"T8227"},{"id":"R4994","pred":"punct","subj":"T8229","obj":"T8210"},{"id":"R4995","pred":"cc","subj":"T8230","obj":"T8210"},{"id":"R4996","pred":"mark","subj":"T8231","obj":"T8232"},{"id":"R4997","pred":"conj","subj":"T8232","obj":"T8210"},{"id":"R4998","pred":"nsubj","subj":"T8233","obj":"T8232"},{"id":"R4999","pred":"prep","subj":"T8234","obj":"T8233"},{"id":"R5000","pred":"compound","subj":"T8235","obj":"T8236"},{"id":"R5001","pred":"pobj","subj":"T8236","obj":"T8234"},{"id":"R5002","pred":"punct","subj":"T8237","obj":"T8232"},{"id":"R5003","pred":"prep","subj":"T8238","obj":"T8232"},{"id":"R5004","pred":"pobj","subj":"T8239","obj":"T8238"},{"id":"R5005","pred":"punct","subj":"T8240","obj":"T8232"},{"id":"R5006","pred":"prep","subj":"T8241","obj":"T8232"},{"id":"R5007","pred":"amod","subj":"T8242","obj":"T8243"},{"id":"R5008","pred":"pobj","subj":"T8243","obj":"T8241"},{"id":"R5009","pred":"compound","subj":"T8244","obj":"T8243"},{"id":"R5010","pred":"punct","subj":"T8245","obj":"T8204"}],"text":"Within 0.5 h of TGF-β2 treatment, Snail promoter activity had increased 3-fold, and by 2 h, it peaked to approximately 10-fold over control levels (Figure 5D). Thereafter, Snail promoter activity rapidly returned to the basal levels seen in unstimulated keratinocytes. The kinetics of Snail promoter activity closely paralleled those observed for Snail protein induction. Moreover, the stimulatory effects appeared to be specific to TGF-β2, since they were abrogated either by heat inactivation of the TGF-β2 protein or by mutation of a putative SMAD binding element located about 1.8 kb 5′ from the Snail transcription start site (Figure 5D). Taken together, these results suggested that in keratinocytes, TGF-β2 signaling results in a pSMAD2-dependent transient activation of the Snail gene, and that maintenance of Snail protein relies, in part, upon sustained promoter activity."}