PMC:539061 / 35309-35877 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/539061","sourcedb":"PMC","sourceid":"539061","source_url":"http://www.ncbi.nlm.nih.gov/pmc/539061","text":"(A) Failure of Wnt and noggin signaling to induce Snail in cultured keratinocytes. Primary mouse keratinocytes were treated with Wnt- and/or noggin-conditioned medium (+) or the corresponding control medium (–). These conditions are known to activate the LEF-1/β-catenin reporter TOPGal and down-regulate the E-cadherin promoter (see [4] for details). Using Western blot analyses, cellular proteins were then analyzed for Snail, LEF-1, β-catenin, and tubulin. Proteins from keratinocytes transfected with K14-Snail were used as a positive control for Snail expression.","tracks":[{"project":"2_test","denotations":[{"id":"15630473-12646922-84810285","span":{"begin":335,"end":336},"obj":"12646922"},{"id":"T81547","span":{"begin":335,"end":336},"obj":"12646922"}],"attributes":[{"subj":"15630473-12646922-84810285","pred":"source","obj":"2_test"},{"subj":"T81547","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ecbf93","default":true}]}]}}