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craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T12420 57858-57862 NCBITaxon:10088 denotes Mice
T12421 57867-57870 PR:000009454 denotes K14
T12422 57871-57876 PR:000015308 denotes Snail
T12423 57880-57885 NCBITaxon:10088 denotes mouse
T12424 57927-57932 PR:000015308 denotes Snail
T12425 57936-57943 SO:0000155 denotes plasmid
T12426 58011-58016 PR:P23940 denotes BamHI
T12427 58049-58052 PR:000009454 denotes K14
T12428 58053-58059 SO:0000440 denotes vector
T12429 58113-58120 GO:0005634 denotes nucleus
T12430 58124-58131 UBERON:0000922 denotes embryos
T12431 58141-58145 NCBITaxon:10088 denotes mice
T12432 58151-58154 PR:000009454 denotes K14
T12433 58155-58161 PR:000000364 denotes Smad 2
T12434 58165-58170 NCBITaxon:10088 denotes mouse
T12435 58209-58215 PR:000000183 denotes TGF-β2
T12436 58230-58235 NCBITaxon:10088 denotes mouse
T12437 58263-58266 PR:000014841 denotes shh
T12438 58270-58275 NCBITaxon:10088 denotes mouse
T12439 58292-58297 NCBITaxon:10088 denotes mouse
T12625 58402-58415 CL:0000312 denotes keratinocytes
T12626 58441-58447 GO:0019835 denotes lysing
T12627 58457-58462 GO:0019835 denotes lysis
T12628 58474-58479 CHEBI:63016 denotes NP-40
T12629 58484-58503 CHEBI:9177 denotes sodium deoxycholate
T12630 58511-58515 CHEBI:9754 denotes Tris
T12631 58516-58518 CHEBI:17996 denotes Cl
T12632 58536-58540 CHEBI:26710 denotes NaCl
T12633 58557-58572 CHEBI:35607 denotes sodium vanadate
T12634 58579-58608 CHEBI:8102 denotes phenylmethylsulfonyl fluoride
T12635 58687-58693 UBERON:0000479 denotes tissue
T12636 58702-58708 UBERON:0000479 denotes tissue
T12637 58843-58855 CHEBI:9750 denotes Triton X-100
T12638 58887-58891 CHEBI:26710 denotes NaCl
T12639 58896-58915 CHEBI:9177 denotes sodium deoxycholate
T12640 58926-58929 CHEBI:8984 denotes SDS
T12641 59303-59311 GO:0042571 denotes antibody
T12642 59481-59486 GO:0019835 denotes lysis
T12643 59523-59531 GO:0042571 denotes antibody
T12644 59532-59539 CHEBI:59132 denotes antigen
T12645 59624-59627 CHEBI:8984 denotes SDS
T12646 59652-59666 CHEBI:53325 denotes nitrocellulose
T13008 59863-59876 CL:0000312 denotes keratinocytes
T13009 59951-59964 GO:0009294 denotes transfections
T13010 59995-60002 CHEBI:33893 denotes reagent
T13011 60146-60154 SO:0000167 denotes promoter
T13012 60353-60360 NCBITaxon:6134 denotes Runella
T13013 60378-60389 GO:0009294 denotes transfected
T13014 60416-60428 GO:0009294 denotes transfection
T13015 60658-60671 CL:0000312 denotes keratinocytes
T13016 60677-60682 UBERON:0001977 denotes serum
T13017 60760-60765 UBERON:0001977 denotes serum
T13018 60800-60806 PR:000000021 denotes noggin
T13242 60879-60885 NCBITaxon:39107 denotes murine
T13243 60886-60891 PR:000015308 denotes Snail
T13244 60892-60900 SO:0000167 denotes promoter
T13245 60930-60944 SO:0000121 denotes forward primer
T13246 61027-61041 SO:0000132 denotes reverse primer
T13247 61116-61121 NCBITaxon:10088 denotes mouse
T13248 61122-61129 SO:0001026 denotes genomic
T13249 61153-61164 SO:0000006 denotes PCR product
T13250 61256-61263 GO:0006266 denotes ligated
T13251 61283-61289 SO:0000440 denotes vector
T13252 61345-61353 SO:0000167 denotes promoter
T13253 61450-61456 SO:0000440 denotes vector
T13254 61554-61569 SO:0000409 denotes binding element
T13255 61669-61683 SO:0000121 denotes forward primer
T13256 61745-61759 SO:0000132 denotes reverse primer
T13257 61835-61840 PR:000015308 denotes Snail
T13258 61849-61862 GO:0097617 denotes hybridization
T13259 61890-61896 SO:0000205 denotes 3′ UTR
T13260 61914-61928 SO:0000121 denotes forward primer
T13261 61970-61984 SO:0000132 denotes reverse primer
T13262 62022-62029 SO:0001026 denotes genomic
T13263 62053-62064 SO:0000006 denotes PCR product
T13264 62086-62093 GO:0006266 denotes ligated
T13265 62113-62119 SO:0000440 denotes vector
T13266 62133-62139 SO:0000417 denotes domain
T13267 62143-62148 PR:000009231 denotes Ajuba
T13268 62249-62255 SO:0000440 denotes vector
T13608 62291-62304 GO:0097617 denotes hybridization
T13609 62323-62329 SO:0000440 denotes vector
T13610 62343-62352 _FRAGMENT denotes region of
T13611 62360-62363 SO:0000837 denotes UTR
T13612 62357-62363 SO:0000205 denotes 3′ UTR
T13613 62367-62372 PR:000015308 denotes Snail
T13614 62408-62419 CHEBI:42098 denotes digoxigenin
T13615 62438-62447 SO:0000077 denotes antisense
T13616 62516-62521 PR:P23940 denotes BamH1
T13617 62542-62556 GO:0097617 denotes hybridizations
T13618 62605-62610 NCBITaxon:10088 denotes mouse
T13619 62611-62618 UBERON:0000922 denotes embryos
T13620 62691-62701 GO:0097617 denotes hybridized
T13621 62733-62743 GO:0097617 denotes hybridized
T13622 62835-62838 CHEBI:42098 denotes dig
T13623 62846-62854 GO:0042571 denotes antibody
T13624 62936-62939 CHEBI:7586 denotes NBT
T13625 62944-62948 CHEBI:75508 denotes BCIP
T13809 63030-63036 UBERON:0000479 denotes Tissue
T13810 63229-63239 GO:0042571 denotes antibodies
T13811 63241-63247 UBERON:0000479 denotes Tissue
T13812 63444-63452 GO:0042571 denotes antibody
T13813 63475-63480 PR:000015308 denotes Snail
T13814 63515-63522 CHEBI:59132 denotes antigen
T13815 63543-63557 CHEBI:53258 denotes sodium citrate
T13816 63571-63578 CHEBI:59132 denotes Antigen
T13980 63780-63793 CL:0000312 denotes keratinocytes
T13981 63802-63808 UBERON:0000479 denotes tissue
T13982 63910-63917 SO:0000112 denotes primers
T13983 63963-63970 SO:0000112 denotes primers
T13984 63989-63994 PR:000015308 denotes Snail
T13985 63995-64002 SO:0001030 denotes forward
T13986 64033-64038 PR:000015308 denotes Snail
T13987 64039-64046 SO:0001031 denotes reverse
T13988 64081-64088 SO:0001030 denotes forward
T13989 64132-64139 SO:0001031 denotes reverse
T66 41-46 PR:000015308 denotes Snail
T67 50-63 UBERON:0002073 denotes Hair Follicle
T68 50-77 GO:0031069 denotes Hair Follicle Morphogenesis
T69 150-163 GO:0048513 denotes organogenesis
T70 200-216 UBERON:0010136 denotes epithelial sheet
T71 295-299 UBERON:0001037 denotes hair
T72 304-317 GO:0009653 denotes morphogenesis
T73 352-357 NCBITaxon:10088 denotes mouse
T74 378-391 CL:0000312 denotes keratinocytes
T75 433-446 GO:0005576 denotes extracellular
T76 491-504 GO:0008283 denotes proliferation
T77 559-588 PR:000000183 denotes transforming growth factor β2
T78 659-664 PR:000015308 denotes Snail
T79 686-693 CHEBI:52290 denotes mitogen
T80 753-762 UBERON:0007376 denotes epidermis
T81 764-769 PR:000015308 denotes Snail
T82 773-783 GO:0010467 denotes expression
T83 798-811 GO:0008283 denotes proliferation
T84 860-870 PR:000001447 denotes E-cadherin
T85 988-1006 GO:0005911 denotes cell-cell contacts
T86 1052-1067 GO:0000187 denotes MAPK activation
T87 1207-1220 GO:0008283 denotes proliferation
T88 1398-1402 UBERON:0001037 denotes hair
T571 1640-1649 NCBITaxon:40674 denotes Mammalian
T572 1675-1688 GO:0009653 denotes morphogenesis
T573 1821-1833 _FRAGMENT denotes formation of
T574 1839-1845 GO:0048513 denotes organs
T575 1839-1845 UBERON:0000062 denotes organs
T576 1857-1862 UBERON:0002048 denotes lungs
T577 1864-1875 UBERON:0002240 denotes spinal cord
T578 1877-1891 UBERON:0001911 denotes mammary glands
T579 1897-1911 UBERON:0002073 denotes hair follicles
T580 1943-1953 UBERON:0000483 denotes epithelial
T581 1943-1959 CL:0000066 denotes epithelial cells
T582 2032-2048 UBERON:0010136 denotes epithelial sheet
T583 2083-2093 UBERON:0003104 denotes mesenchyme
T584 2103-2116 GO:0009653 denotes morphogenesis
T585 2171-2181 UBERON:0000483 denotes epithelium
T586 2186-2196 UBERON:0003104 denotes mesenchyme
T587 2228-2233 UBERON:0000062 denotes organ
T588 2256-2262 GO:0065007 denotes govern
T589 2339-2364 PR:000000034 denotes bone morphogenic proteins
T590 2366-2370 PR:000000034 denotes BMPs
T591 2373-2402 PR:000000046 denotes transforming growth factor βs
T592 2404-2410 PR:000000046 denotes TGF-βs
T593 2417-2427 CL:0000057 denotes fibroblast
T594 2473-2485 GO:0009986 denotes cell surface
T595 2491-2499 GO:0016020 denotes membrane
T596 2517-2525 GO:0005576 denotes external
T597 2526-2545 CHEBI:62488 denotes signaling molecules
T598 2563-2595 GO:0035556 denotes cascades of intracellular events
T599 2575-2588 GO:0005622 denotes intracellular
T600 2625-2629 SO:0000704 denotes gene
T601 2625-2640 GO:0010467 denotes gene expression
T602 2787-2800 GO:0009653 denotes morphogenetic
T603 3002-3011 CHEBI:35224 denotes effectors
T604 3188-3204 UBERON:0010136 denotes epithelial sheet
T605 3260-3273 _FRAGMENT denotes proliferation
T606 3309-3311 _FRAGMENT denotes of
T607 3321-3326 GO:0008283 denotes cells
T608 3396-3409 GO:0005576 denotes extracellular
T609 3410-3420 UBERON:0000483 denotes epithelial
T610 3421-3432 UBERON:0003104 denotes mesenchymal
T611 3490-3506 _FRAGMENT denotes determination of
T612 3516-3526 GO:0001709 denotes cell fates
T613 3644-3650 UBERON:0000062 denotes organs
T614 3652-3666 UBERON:0002073 denotes hair follicles
T615 3708-3722 UBERON:0005153 denotes epithelial bud
T616 3734-3743 NCBITaxon:40674 denotes Mammalian
T617 3744-3759 UBERON:0019204 denotes skin epithelium
T618 3800-3810 UBERON:0000924 denotes ectodermal
T619 3800-3816 CL:0000221 denotes ectodermal cells
T620 3850-3861 UBERON:0003104 denotes mesenchymal
T621 3850-3867 CL:0000134 denotes mesenchymal cells
T622 3941-3951 UBERON:0000483 denotes epithelial
T623 3952-3963 UBERON:0003104 denotes mesenchymal
T624 4040-4050 UBERON:0000483 denotes epithelial
T625 4040-4056 CL:0000066 denotes epithelial cells
T626 4103-4114 UBERON:0003104 denotes mesenchymal
T627 4103-4120 CL:0000134 denotes mesenchymal cells
T628 4154-4168 UBERON:0000412 denotes dermal papilla
T629 4211-4224 UBERON:0002073 denotes hair follicle
T630 4279-4293 UBERON:0000412 denotes dermal papilla
T631 4355-4361 UBERON:0002067 denotes dermis
T632 4508-4518 UBERON:0000483 denotes epithelial
T633 4523-4534 UBERON:0003104 denotes mesenchymal
T634 4557-4560 PR:000000034 denotes BMP
T635 4579-4585 PR:000000021 denotes noggin
T636 4617-4643 PR:000009751 denotes lymphoid enhancer factor-1
T637 4626-4634 SO:0000165 denotes enhancer
T638 4644-4653 PR:000002198 denotes β-catenin
T639 4655-4660 PR:000009751 denotes LEF-1
T640 4661-4670 PR:000002198 denotes β-catenin
T641 4681-4685 SO:0000704 denotes gene
T642 4711-4727 UBERON:0011817 denotes follicle placode
T643 4854-4858 SO:0000704 denotes gene
T644 4868-4878 PR:000001447 denotes E-cadherin
T645 4897-4907 UBERON:0000483 denotes epithelial
T646 4908-4916 PR:000001327 denotes cadherin
T647 4937-4945 GO:0016020 denotes membrane
T648 4954-4986 GO:0005913 denotes intercellular adherens junctions
T649 4988-4991 GO:0005912 denotes AJs
T650 5031-5041 PR:000001447 denotes E-cadherin
T651 5072-5077 NCBITaxon:10088 denotes mouse
T652 5084-5097 UBERON:0002073 denotes hair follicle
T653 5084-5111 GO:0031069 denotes hair follicle morphogenesis
T654 5140-5150 PR:000001447 denotes E-cadherin
T655 5190-5199 GO:0065007 denotes governing
T656 5244-5257 GO:0009653 denotes morphogenesis
T657 5268-5273 PR:000009751 denotes LEF-1
T658 5275-5285 PR:000001447 denotes E-cadherin
T659 5300-5309 PR:000002198 denotes β-catenin
T660 5351-5361 PR:000001447 denotes E-cadherin
T661 5362-5371 PR:000002198 denotes β-catenin
T662 5372-5381 GO:0032991 denotes complexes
T663 5442-5462 GO:0030041 denotes actin polymerization
T664 5448-5462 MOP:0000629 denotes polymerization
T665 5503-5506 GO:0005912 denotes AJs
T666 5525-5537 GO:0005856 denotes cytoskeleton
T667 5548-5564 UBERON:0010136 denotes epithelial sheet
T668 5612-5617 PR:P20154 denotes Lin-1
T669 5619-5624 PR:000009116 denotes Isl-1
T670 5626-5631 PR:P09088 denotes Mec-3
T671 5646-5651 PR:000009231 denotes Ajuba
T672 5669-5674 PR:000018242 denotes zyxin
T673 5775-5782 CHEBI:52290 denotes mitogen
T674 5852-5855 GO:0005912 denotes AJs
T675 5892-5904 GO:0005856 denotes cytoskeletal
T676 5930-5937 GO:0005634 denotes nuclear
T677 5942-5953 GO:0005737 denotes cytoplasmic
T678 6015-6025 PR:000001447 denotes E-cadherin
T679 6169-6178 GO:0065007 denotes governing
T680 6179-6189 PR:000001447 denotes E-cadherin
T681 6190-6198 SO:0000167 denotes promoter
T682 6257-6262 PR:000009751 denotes LEF-1
T683 6263-6272 PR:000002198 denotes β-catenin
T684 6273-6285 SO:0000409 denotes binding site
T685 6314-6319 PR:000015308 denotes Snail
T686 6320-6324 PR:000015309 denotes Slug
T687 6414-6419 PR:000015308 denotes Snail
T688 6443-6453 PR:000001447 denotes E-cadherin
T689 6476-6486 UBERON:0000483 denotes epithelial
T690 6476-6513 GO:0001837 denotes epithelial to mesenchymal transitions
T691 6490-6501 UBERON:0003104 denotes mesenchymal
T692 6515-6519 GO:0001837 denotes EMTs
T693 6576-6586 UBERON:0000483 denotes epithelial
T694 6576-6592 CL:0000066 denotes epithelial cells
T695 6598-6604 _FRAGMENT denotes motile
T696 6617-6622 CL:0000219 denotes cells
T697 6605-6616 UBERON:0003104 denotes mesenchymal
T698 6605-6622 CL:0000134 denotes mesenchymal cells
T699 6662-6665 GO:0001837 denotes EMT
T700 6674-6684 PR:000001447 denotes E-cadherin
T701 6867-6875 PR:000001327 denotes cadherin
T702 6876-6886 GO:0010467 denotes expression
T703 6917-6922 PR:000015308 denotes Snail
T704 6961-6966 PR:000009751 denotes LEF-1
T705 6967-6976 PR:000002198 denotes β-catenin
T706 7002-7012 PR:000001447 denotes E-cadherin
T707 7013-7021 SO:0000167 denotes promoter
T708 7077-7082 PR:000015308 denotes Snail
T709 7086-7095 GO:0010467 denotes expressed
T710 7125-7129 UBERON:0001037 denotes hair
T711 7150-7160 PR:000001447 denotes E-cadherin
T712 7195-7208 GO:0008283 denotes proliferation
T713 7233-7238 PR:000015308 denotes Snail
T714 7458-7462 UBERON:0001037 denotes hair
T715 7507-7517 PR:000001447 denotes E-cadherin
T716 7529-7542 GO:0008283 denotes proliferation
T717 7615-7620 PR:000015308 denotes Snail
T718 7628-7632 UBERON:0001037 denotes hair
T719 7688-7701 GO:0016055 denotes Wnt signaling
T720 7706-7709 PR:000000034 denotes BMP
T721 7722-7727 PR:000009751 denotes LEF-1
T722 7728-7737 PR:000002198 denotes β-catenin
T723 7775-7780 PR:000015308 denotes Snail
T724 7781-7785 SO:0000704 denotes gene
T725 7781-7796 GO:0010467 denotes gene expression
T726 7800-7809 UBERON:0000922 denotes embryonic
T727 7815-7828 CL:0000312 denotes keratinocytes
T728 7885-7889 SO:0000704 denotes gene
T729 7922-7928 PR:000000183 denotes TGF-β2
T730 7933-8003 PR:000000364 denotes small phenotype– and mothers against decapentaplegic–related protein 2
T731 8005-8010 PR:000000364 denotes SMAD2
T732 8047-8052 PR:000015308 denotes Snail
T733 8053-8057 SO:0000704 denotes gene
T734 8053-8068 GO:0010467 denotes gene expression
T735 8072-8087 UBERON:0019204 denotes skin epithelium
T736 8107-8113 PR:000000183 denotes TGF-β2
T737 8128-8133 PR:000015308 denotes Snail
T738 8134-8138 SO:0000704 denotes gene
T739 8134-8149 GO:0010467 denotes gene expression
T740 8151-8164 UBERON:0005086 denotes hair placodes
T741 8255-8260 PR:000015308 denotes Snail
T742 8292-8315 GO:0001708 denotes cell fate specification
T743 8369-8382 GO:0008283 denotes proliferation
T2400 8408-8413 PR:000015308 denotes Snail
T2401 8435-8444 GO:0010467 denotes Expressed
T2402 8464-8468 UBERON:0001037 denotes Hair
T2403 8491-8504 GO:0009653 denotes Morphogenesis
T2404 8514-8519 PR:000015308 denotes Snail
T2405 8571-8575 GO:0001837 denotes EMTs
T2406 8692-8715 GO:0005911 denotes intercellular junctions
T2407 8768-8773 PR:000015308 denotes Snail
T2408 8836-8846 UBERON:0000483 denotes epithelial
T2409 8878-8891 UBERON:0002073 denotes hair follicle
T2410 8958-8963 PR:000015308 denotes Snail
T2411 8985-8990 PR:000009751 denotes LEF-1
T2412 8991-9000 PR:000002198 denotes β-catenin
T2413 9020-9030 PR:000001447 denotes E-cadherin
T2414 9031-9041 GO:0010467 denotes expression
T2415 9045-9058 CL:0000312 denotes keratinocytes
T2416 9109-9114 PR:000009751 denotes LEF-1
T2417 9115-9124 PR:000002198 denotes β-catenin
T2418 9128-9141 UBERON:0002073 denotes hair follicle
T2419 9128-9155 GO:0031069 denotes hair follicle morphogenesis
T2420 9196-9201 PR:000015308 denotes Snail
T2421 9202-9206 PR:000015309 denotes Slug
T2422 9295-9300 PR:000015308 denotes Snail
T2423 9306-9316 GO:0010467 denotes expression
T2424 9341-9354 GO:0009790 denotes embryogenesis
T2425 9369-9383 UBERON:0002073 denotes hair follicles
T2426 9446-9451 PR:000015308 denotes Snail
T2427 9460-9469 GO:0010467 denotes expressed
T2428 9515-9528 GO:0097617 denotes hybridization
T2429 9562-9567 PR:000015308 denotes Snail
T2430 9568-9590 SO:0000205 denotes 3′ untranslated region
T2431 9568-9570 _FRAGMENT denotes 3′
T2432 9592-9595 SO:0000205 denotes UTR
T2433 9573-9583 GO:0006412 denotes translated
T2434 9598-9607 UBERON:0000922 denotes Embryonic
T2435 9674-9687 GO:0009653 denotes morphogenesis
T2436 9734-9739 PR:000015308 denotes Snail
T2437 9740-9750 GO:0010467 denotes expression
T2438 9819-9832 GO:0097617 denotes hybridization
T2439 9857-9867 UBERON:0000483 denotes epithelium
T2440 9879-9883 UBERON:0001037 denotes hair
T2441 10017-10030 GO:0097617 denotes hybridization
T2442 10068-10073 PR:000015308 denotes Snail
T2443 10079-10089 GO:0010467 denotes expression
T2444 10139-10149 GO:0097617 denotes hybridized
T2445 10213-10226 GO:0009653 denotes morphogenesis
T2446 10251-10262 GO:0097617 denotes Hybridizing
T2447 10263-10267 UBERON:0001037 denotes hair
T2448 10319-10330 GO:0097617 denotes hybridizing
T2449 12805-12810 PR:000015308 denotes Snail
T2450 12816-12826 GO:0010467 denotes expression
T2451 12878-12886 GO:0042571 denotes antibody
T2452 12975-12982 SO:0000417 denotes domains
T2453 13024-13032 GO:0042571 denotes antibody
T2454 13082-13095 CL:0000312 denotes keratinocytes
T2455 13147-13160 CL:0000312 denotes keratinocytes
T2456 13173-13183 GO:0010467 denotes expressing
T2457 13212-13217 PR:000015308 denotes Snail
T2458 13243-13251 GO:0042571 denotes antibody
T2459 13315-13320 PR:000015308 denotes Snail
T2460 13360-13369 UBERON:0000922 denotes embryonic
T2461 13370-13375 NCBITaxon:10088 denotes mouse
T2462 13444-13457 UBERON:0002073 denotes hair follicle
T2463 13444-13471 GO:0031069 denotes hair follicle morphogenesis
T2464 13515-13519 UBERON:0010164 denotes hair
T2465 13527-13532 NCBITaxon:10088 denotes mouse
T2466 13639-13644 PR:000015308 denotes Snail
T2467 13669-13678 UBERON:0007376 denotes epidermis
T2468 13759-13772 _FRAGMENT denotes commitment of
T2469 13785-13790 _FRAGMENT denotes cells
T2470 13817-13819 _FRAGMENT denotes to
T2471 13827-13836 GO:0045165 denotes cell fate
T2472 13785-13793 _FRAGMENT denotes cells of
T2473 13808-13816 CL:0000221 denotes ectoderm
T2474 13798-13816 UBERON:0000924 denotes embryonic ectoderm
T2475 13822-13826 UBERON:0001037 denotes hair
T2476 13822-13831 CL:0002559 denotes hair cell
T2477 13878-13891 GO:0097617 denotes hybridization
T2478 13906-13911 PR:000015308 denotes Snail
T2479 13912-13920 GO:0042571 denotes antibody
T2480 13934-13938 _FRAGMENT denotes hair
T2481 13952-13961 UBERON:0002073 denotes follicles
T2482 14037-14042 PR:000015308 denotes Snail
T2483 14043-14051 GO:0042571 denotes antibody
T2484 14124-14129 PR:000015308 denotes Snail
T2485 14157-14166 GO:0010467 denotes expressed
T2486 14170-14183 CL:0000312 denotes keratinocytes
T2487 14273-14278 PR:000015308 denotes Snail
T2488 14287-14300 GO:0097617 denotes hybridization
T2489 14326-14331 PR:000015308 denotes Snail
T2490 14332-14342 GO:0010467 denotes expression
T2491 14350-14354 UBERON:0001037 denotes hair
T2492 14413-14418 PR:000015308 denotes Snail
T2493 14448-14457 _FRAGMENT denotes nuclei of
T2494 14471-14476 GO:0005634 denotes cells
T2495 14462-14466 UBERON:0001037 denotes hair
T2496 14523-14528 PR:000015308 denotes Snail
T2497 14605-14610 PR:000015308 denotes Snail
T2498 14683-14696 GO:0009653 denotes morphogenesis
T2499 14698-14703 PR:000015308 denotes Snail
T2500 14733-14744 UBERON:0010512 denotes guard hairs
T2501 14774-14779 UBERON:0001037 denotes hairs
T2502 14841-14846 NCBITaxon:10088 denotes mouse
T2503 14847-14851 UBERON:0010166 denotes coat
T2504 14909-14919 GO:0042571 denotes antibodies
T2505 14932-14945 GO:0008283 denotes proliferating
T2506 14946-14953 GO:0005634 denotes nuclear
T2507 14954-14961 CHEBI:59132 denotes antigen
T2508 14962-14966 PR:000010425 denotes Ki67
T2509 14982-14987 PR:000015308 denotes Snail
T2510 14988-14998 GO:0010467 denotes expression
T2511 15070-15083 GO:0008283 denotes proliferation
T2512 15139-15149 GO:0042571 denotes antibodies
T2513 15230-15243 GO:0008283 denotes proliferating
T2514 15245-15249 PR:000010425 denotes Ki67
T2515 15313-15317 UBERON:0001037 denotes hair
T2516 15349-15354 PR:000015308 denotes Snail
T2517 15369-15373 PR:000010425 denotes Ki67
T2518 15402-15406 UBERON:0001037 denotes hair
T2519 15455-15460 PR:000009751 denotes LEF-1
T2520 15461-15470 PR:000002198 denotes β-catenin
T2521 15506-15518 UBERON:0005086 denotes hair placode
T2522 15555-15559 UBERON:0001037 denotes hair
T2523 15575-15593 _FRAGMENT denotes down-regulation in
T2524 15605-15615 GO:0010629 denotes expression
T2525 15594-15604 PR:000001447 denotes E-cadherin
T3694 15654-15664 GO:0010467 denotes Expression
T3695 15668-15673 PR:000015308 denotes Snail
T3696 15685-15694 UBERON:0007376 denotes Epidermal
T3697 15700-15713 GO:0008283 denotes proliferation
T3698 15748-15753 NCBITaxon:10088 denotes Mouse
T3699 15781-15786 PR:000015308 denotes Snail
T3700 15787-15797 GO:0010467 denotes expression
T3701 15814-15818 UBERON:0001037 denotes hair
T3702 15846-15859 GO:0008283 denotes proliferation
T3703 15915-15925 GO:0010467 denotes expressing
T3704 15926-15931 PR:000015308 denotes Snail
T3705 15945-15950 NCBITaxon:10088 denotes mouse
T3706 15951-15965 UBERON:0001003 denotes skin epidermis
T3707 16001-16006 PR:000015308 denotes Snail
T3708 16062-16067 PR:000015308 denotes Snail
T3709 16107-16110 PR:000009454 denotes K14
T3710 16111-16116 PR:000015308 denotes Snail
T3711 16124-16131 NCBITaxon:33208 denotes animals
T3712 16149-16158 GO:0010467 denotes expressed
T3713 16163-16172 SO:0000902 denotes transgene
T3714 16213-16217 NCBITaxon:10088 denotes Mice
T3715 16238-16247 SO:0000902 denotes transgene
T3716 16273-16277 GO:0016265 denotes died
T3717 16298-16303 GO:0007567 denotes birth
T3718 16341-16345 NCBITaxon:10088 denotes mice
T3719 16355-16364 SO:0000902 denotes transgene
T3720 16418-16423 PR:000015308 denotes Snail
T3721 16424-16428 SO:0000704 denotes gene
T3722 16424-16439 GO:0010467 denotes gene expression
T3723 16566-16571 GO:0007567 denotes birth
T3724 16576-16581 PR:000015308 denotes Snail
T3725 16585-16592 NCBITaxon:33208 denotes animals
T3726 16593-16597 GO:0040007 denotes grew
T3727 16652-16657 UBERON:0002415 denotes tails
T3728 16725-16729 NCBITaxon:10088 denotes mice
T3729 16764-16773 UBERON:0007376 denotes epidermal
T3730 16853-16858 PR:000015308 denotes Snail
T3731 16905-16915 GO:0010467 denotes expressing
T3732 16916-16923 GO:0005634 denotes nuclear
T3733 16934-16939 PR:000015308 denotes Snail
T3734 17005-17018 GO:0008283 denotes proliferation
T3735 17035-17045 GO:0042571 denotes antibodies
T3736 17058-17071 GO:0008283 denotes proliferating
T3737 17072-17079 GO:0005634 denotes nuclear
T3738 17080-17087 CHEBI:59132 denotes antigen
T3739 17088-17092 PR:000010425 denotes Ki67
T3740 17214-17224 GO:0010467 denotes expressing
T3741 17261-17278 UBERON:0010402 denotes suprabasal layers
T3742 19310-19320 GO:0010467 denotes Expression
T3743 19328-19333 PR:000015308 denotes Snail
T3744 19334-19343 SO:0000902 denotes transgene
T3745 19409-19418 UBERON:0007376 denotes epidermis
T3746 19486-19499 GO:0008283 denotes proliferating
T3747 19512-19517 PR:000015308 denotes Snail
T3748 19518-19528 GO:0010467 denotes expression
T3749 19557-19563 _FRAGMENT denotes layers
T3750 19569-19576 UBERON:0002026 denotes spinous
T3751 19664-19673 PR:000009481 denotes keratin 5
T3752 19675-19677 PR:000009481 denotes K5
T3753 19717-19728 UBERON:0002025 denotes basal layer
T3754 19769-19774 PR:000015308 denotes Snail
T3755 19780-19784 NCBITaxon:10088 denotes mice
T3756 19801-19813 GO:0007369 denotes gastrulation
T3757 19862-19867 PR:000015308 denotes Snail
T3758 19880-19896 GO:0043588 denotes skin development
T3759 19937-19947 GO:0010467 denotes expression
T3760 19951-19956 PR:000015308 denotes Snail
T3761 19986-19988 PR:000009481 denotes K5
T3762 19990-19994 PR:000010425 denotes Ki67
T3763 20006-20018 UBERON:0010402 denotes suprabasally
T3764 21927-21940 GO:0008283 denotes proliferation
T3765 22010-22020 UBERON:0000483 denotes epithelial
T3766 22055-22064 UBERON:0007376 denotes epidermal
T3767 22112-22117 PR:000015308 denotes Snail
T3768 22122-22131 GO:0010467 denotes expressed
T3769 22383-22393 GO:0042571 denotes antibodies
T3770 22420-22434 GO:0030056 denotes hemidesmosomes
T3771 22469-22490 CL:0002187 denotes basal epidermal cells
T3772 22475-22484 UBERON:0007376 denotes epidermal
T3773 22624-22628 UBERON:0001037 denotes hair
T3774 22636-22645 UBERON:0000922 denotes embryonic
T3775 22658-22662 NCBITaxon:10088 denotes mice
T3776 22668-22673 PR:000015308 denotes Snail
T3777 22686-22695 GO:0010467 denotes expressed
T3778 22748-22757 UBERON:0007376 denotes epidermis
T3779 22767-22773 UBERON:0002067 denotes dermis
T3780 22797-22802 UBERON:0007023 denotes adult
T3781 22806-22813 NCBITaxon:33208 denotes animals
T3782 22895-22904 UBERON:0007376 denotes epidermis
T4751 22958-22967 UBERON:0007376 denotes Epidermal
T4752 22973-22986 GO:0008283 denotes proliferation
T4753 23010-23012 GO:0005912 denotes AJ
T4754 23025-23030 PR:000015308 denotes Snail
T4755 23034-23038 NCBITaxon:10088 denotes Mice
T4756 23054-23064 PR:000001447 denotes E-cadherin
T4757 23065-23073 SO:0000167 denotes promoter
T4758 23097-23102 PR:000015308 denotes Snail
T4759 23152-23162 PR:000001447 denotes E-cadherin
T4760 23185-23190 PR:000015308 denotes Snail
T4761 23191-23201 GO:0010467 denotes expressing
T4762 23202-23206 UBERON:0001037 denotes hair
T4763 23239-23249 PR:000001447 denotes E-cadherin
T4764 23260-23262 GO:0005912 denotes AJ
T4765 23309-23318 UBERON:0007376 denotes epidermis
T4766 23328-23333 PR:000015308 denotes Snail
T4767 23408-23418 PR:000001447 denotes E-cadherin
T4768 23489-23497 GO:0042571 denotes antibody
T4769 23521-23523 GO:0005912 denotes AJ
T4770 23534-23543 PR:000002198 denotes β-catenin
T4771 23548-23553 PR:000009231 denotes Ajuba
T4772 23653-23662 PR:000002198 denotes β-catenin
T4773 23667-23672 PR:000009231 denotes Ajuba
T4774 23696-23707 GO:0005737 denotes cytoplasmic
T4775 23723-23740 GO:0005911 denotes cell-cell borders
T4776 27772-27781 UBERON:0007376 denotes epidermis
T4777 27866-27869 UBERON:0001690 denotes ear
T4778 27904-27909 PR:000015308 denotes Snail
T4779 27923-27932 GO:0010467 denotes expressed
T4780 27972-27976 UBERON:0001137 denotes back
T4781 27986-27989 UBERON:0001690 denotes ear
T4782 28014-28024 PR:000001447 denotes E-cadherin
T4783 28076-28085 PR:000002198 denotes β-catenin
T4784 28090-28095 PR:000009231 denotes Ajuba
T4785 28364-28369 PR:000015308 denotes Snail
T4786 28390-28392 GO:0005912 denotes AJ
T4787 28390-28402 GO:0034333 denotes AJ formation
T4788 28429-28439 PR:000001447 denotes E-cadherin
T4789 28440-28444 SO:0000704 denotes gene
T4790 28440-28455 GO:0010467 denotes gene expression
T4791 28567-28577 GO:0010467 denotes expression
T4792 28581-28591 PR:000001447 denotes E-cadherin
T4793 28595-28600 PR:000015308 denotes Snail
T4794 28601-28611 GO:0010467 denotes expressing
T4795 28612-28625 CL:0000312 denotes keratinocytes
T4796 28662-28673 GO:0009294 denotes transfected
T4797 28674-28687 CL:0000312 denotes keratinocytes
T4798 28688-28698 GO:0010467 denotes expressing
T4799 28709-28714 PR:000015308 denotes Snail
T4800 28735-28745 PR:000001447 denotes E-cadherin
T4801 28763-28780 GO:0005911 denotes cell-cell borders
T4802 28784-28794 GO:0010467 denotes expression
T4803 28818-28828 PR:000001447 denotes E-cadherin
T4804 28846-28862 GO:0005911 denotes cell-cell border
T4805 28879-28889 PR:000001447 denotes E-cadherin
T4806 28920-28936 GO:0005911 denotes cell-cell border
T4807 29005-29015 GO:0010467 denotes expression
T4808 29087-29092 PR:000015308 denotes Snail
T4809 29196-29206 PR:000001447 denotes E-cadherin
T4810 29227-29238 GO:0006412 denotes translation
T4811 29289-29291 GO:0005912 denotes AJ
T4812 29332-29341 GO:0016020 denotes membranes
T4813 29346-29369 GO:0005911 denotes intercellular junctions
T4814 29475-29490 UBERON:0019204 denotes skin epithelium
T4815 29513-29517 UBERON:0001037 denotes hair
T4816 29677-29682 PR:000015308 denotes Snail
T4817 29686-29695 UBERON:0007376 denotes epidermis
T4818 29700-29704 UBERON:0001037 denotes hair
T4819 29804-29806 GO:0005912 denotes AJ
T4820 29892-29897 PR:000015308 denotes Snail
T4821 29901-29910 UBERON:0007376 denotes epidermis
T4822 29990-30000 PR:000001447 denotes E-cadherin
T4823 30020-30025 PR:000015308 denotes Snail
T4824 30047-30060 GO:0008283 denotes proliferation
T4825 30104-30115 GO:0009294 denotes transfected
T4826 30116-30129 CL:0000312 denotes keratinocytes
T4827 30130-30140 GO:0010467 denotes expressing
T4828 30141-30146 PR:000015308 denotes Snail
T4829 30237-30247 GO:0010467 denotes expression
T4830 30251-30261 PR:000001447 denotes E-cadherin
T4831 30267-30272 PR:000015308 denotes Snail
T4832 30363-30365 GO:0005912 denotes AJ
T4833 30402-30413 GO:0051235 denotes sequestered
T4834 30421-30436 GO:0005886 denotes plasma membrane
T4835 30458-30471 GO:0008283 denotes proliferation
T4836 30495-30498 GO:0005912 denotes AJs
T4837 30572-30582 PR:000001447 denotes E-cadherin
T4838 30590-30603 _FRAGMENT denotes translocation
T4839 30617-30619 _FRAGMENT denotes to
T4840 30624-30631 GO:0051170 denotes nucleus
T4841 30607-30616 PR:000002198 denotes β-catenin
T4842 30624-30631 GO:0005634 denotes nucleus
T4843 30657-30662 SO:0000704 denotes genes
T4844 30672-30681 GO:0065007 denotes regulated
T4845 30689-30694 PR:000009751 denotes LEF-1
T4846 30695-30701 CL:0000084 denotes T cell
T4847 30774-30781 GO:0005634 denotes nuclear
T4848 30813-30818 PR:000015308 denotes Snail
T4849 30822-30831 UBERON:0007376 denotes epidermis
T4850 30903-30907 SO:0000704 denotes gene
T4851 30934-30943 PR:000002198 denotes β-catenin
T4852 30993-31000 GO:0005634 denotes nuclear
T4853 31001-31010 PR:000002198 denotes β-catenin
T4854 31021-31030 UBERON:0007376 denotes epidermis
T4855 31036-31042 GO:0007618 denotes mating
T4856 31047-31052 PR:000015308 denotes Snail
T4857 31056-31060 NCBITaxon:10088 denotes mice
T4858 31089-31094 NCBITaxon:10088 denotes mouse
T4859 31125-31130 PR:000009751 denotes LEF-1
T4860 31135-31144 PR:000002198 denotes β-catenin
T4861 31208-31219 GO:0005737 denotes cytoplasmic
T4862 31220-31225 PR:000009231 denotes Ajuba
T4863 31295-31300 PR:000015308 denotes Snail
T4864 31304-31313 UBERON:0007376 denotes epidermis
T4865 31377-31382 PR:000009231 denotes Ajuba
T4866 31407-31445 PR:000008224 denotes growth factor receptor-bound protein-2
T4867 31447-31452 PR:000008224 denotes Grb-2
T4868 31454-31470 PR:P26675 denotes son of sevenless
T4869 31472-31475 PR:P26675 denotes Sos
T4870 31541-31559 GO:0000187 denotes activation of MAPK
T4871 31647-31652 PR:000009231 denotes Ajuba
T4872 31695-31700 PR:000015308 denotes Snail
T4873 31701-31711 GO:0010467 denotes expressing
T4874 31712-31721 UBERON:0007376 denotes epidermis
T4875 31738-31743 PR:000009231 denotes Ajuba
T4876 31773-31778 PR:000008224 denotes Grb-2
T4877 31831-31836 PR:000015308 denotes Snail
T4878 31840-31844 NCBITaxon:10088 denotes mice
T4879 31891-31898 NCBITaxon:33208 denotes animals
T4880 31969-31978 UBERON:0007376 denotes epidermis
T4881 31990-31995 PR:000008224 denotes Grb-2
T4882 31996-32001 PR:000009231 denotes Ajuba
T4883 32222-32227 PR:000015308 denotes Snail
T4884 32256-32266 PR:000001447 denotes E-cadherin
T4885 32313-32318 PR:000009231 denotes Ajuba
T4886 32336-32341 PR:000008224 denotes Grb-2
T4887 32342-32345 PR:P26675 denotes Sos
T4888 32374-32379 PR:000008224 denotes Grb-2
T4889 32380-32383 PR:P26675 denotes Sos
T4890 32402-32407 PR:000009231 denotes Ajuba
T4891 32470-32482 CL:0000312 denotes keratinocyte
T4892 32493-32503 GO:0010467 denotes expression
T4893 32507-32512 PR:000009231 denotes Ajuba
T4894 32610-32623 CL:0000312 denotes keratinocytes
T4895 32638-32643 UBERON:0001977 denotes serum
T4896 32652-32665 CL:0000312 denotes keratinocytes
T4897 32683-32694 GO:0009294 denotes transfected
T4898 32703-32708 PR:000009231 denotes Ajuba
T4899 32709-32719 GO:0010467 denotes expression
T4900 32720-32726 SO:0000440 denotes vector
T4901 32800-32811 GO:0009294 denotes transfected
T4902 32821-32824 PR:000009454 denotes K14
T4903 32833-32842 SO:0000902 denotes transgene
T4904 32931-32940 CHEBI:35222 denotes inhibitor
T4905 32974-32979 PR:000008224 denotes Grb-2
T4906 32980-32983 PR:P26675 denotes Sos
T4907 33038-33043 PR:000009231 denotes Ajuba
T4908 33054-33060 SO:0000417 denotes domain
T4909 33097-33102 PR:000008224 denotes Grb-2
T4910 33105-33108 PR:000027234 denotes Src
T4911 33109-33117 SO:0000857 denotes homology
T4912 33120-33126 SO:0000417 denotes domain
T4913 33142-33148 SO:0000417 denotes domain
T4914 33157-33166 GO:0010467 denotes expressed
T4915 33170-33175 UBERON:0001977 denotes serum
T4916 33184-33197 CL:0000312 denotes keratinocytes
T4917 33288-33297 CHEBI:35222 denotes inhibitor
T4918 33318-33323 PR:000008224 denotes Grb-2
T4919 33324-33327 PR:P26675 denotes Sos
T4920 33400-33409 GO:0005829 denotes cytosolic
T4921 33410-33415 PR:000009231 denotes Ajuba
T4922 33424-33429 PR:000009231 denotes Ajuba
T4923 33440-33446 SO:0000417 denotes domain
T4924 33466-33471 PR:000008224 denotes Grb-2
T4925 33472-33475 PR:P26675 denotes Sos
T4926 33640-33645 PR:000015308 denotes Snail
T4927 33646-33656 GO:0010467 denotes expression
T4928 33661-33674 GO:0008283 denotes proliferation
T4929 33693-33708 UBERON:0019204 denotes skin epithelium
T4930 33746-33749 GO:0005912 denotes AJs
T6799 33914-33927 _FRAGMENT denotes Regulation of
T6800 33934-33949 GO:0010468 denotes Gene Expression
T6801 33928-33933 PR:000015308 denotes Snail
T6802 33934-33938 SO:0000704 denotes Gene
T6803 33979-33985 PR:000000183 denotes TGF-β2
T6804 34014-34018 UBERON:0001037 denotes Hair
T6805 34045-34050 PR:000015308 denotes Snail
T6806 34056-34066 GO:0010467 denotes expression
T6807 34074-34078 UBERON:0001037 denotes hair
T6808 34129-34139 GO:0065007 denotes regulating
T6809 34144-34149 PR:000015308 denotes Snail
T6810 34150-34154 SO:0000704 denotes gene
T6811 34169-34182 GO:0005576 denotes extracellular
T6812 34232-34242 GO:0010467 denotes expression
T6813 34256-34261 PR:000015308 denotes Snail
T6814 34312-34316 PR:000000034 denotes BMPs
T6815 34328-34334 PR:000000046 denotes TGF-βs
T6816 34348-34352 UBERON:0001037 denotes hair
T6817 34386-34391 PR:000015308 denotes Snail
T6818 34399-34408 GO:0010467 denotes expressed
T6819 34426-34439 CL:0000312 denotes keratinocytes
T6820 34445-34452 GO:0046903 denotes secrete
T6821 34460-34464 PR:000000034 denotes BMPs
T6822 34519-34522 _FRAGMENT denotes Wnt
T6823 34533-34542 GO:0016055 denotes signaling
T6824 34527-34532 PR:000000046 denotes TGF-β
T6825 34527-34542 GO:0007179 denotes TGF-β signaling
T6826 34573-34578 PR:000015308 denotes Snail
T6827 34663-34669 PR:000017443 denotes Wnt-3a
T6828 34689-34702 CL:0000312 denotes keratinocytes
T6829 34749-34752 PR:000000034 denotes BMP
T6830 34753-34762 CHEBI:35222 denotes inhibitor
T6831 34763-34769 PR:000000021 denotes noggin
T6832 34785-34790 PR:000009751 denotes LEF-1
T6833 34791-34801 GO:0010467 denotes expression
T6834 34853-34863 PR:000001447 denotes E-cadherin
T6835 34864-34872 SO:0000167 denotes promoter
T6836 34887-34892 PR:000009751 denotes LEF-1
T6837 34893-34902 PR:000002198 denotes β-catenin
T6838 34922-34926 SO:0000704 denotes gene
T6839 34955-34960 PR:000015308 denotes Snail
T6840 34961-34971 GO:0010467 denotes expression
T6841 35064-35070 PR:000000021 denotes noggin
T6842 35074-35087 UBERON:0002073 denotes hair follicle
T6843 35180-35189 GO:0065007 denotes governing
T6844 35190-35195 PR:000015308 denotes Snail
T6845 35196-35206 GO:0010467 denotes expression
T6846 35210-35223 CL:0000312 denotes keratinocytes
T6847 37336-37342 PR:000000182 denotes TGF-β1
T6848 37368-37373 PR:000015308 denotes Snail
T6849 37392-37403 CL:0000182 denotes hepatocytes
T6850 37408-37413 UBERON:0000948 denotes heart
T6851 37427-37440 CL:0000312 denotes keratinocytes
T6852 37451-37457 PR:000000182 denotes TGF-β1
T6853 37467-37479 CL:0000312 denotes keratinocyte
T6854 37563-37576 UBERON:0002073 denotes hair follicle
T6855 37642-37647 PR:000000046 denotes TGF-β
T6856 37648-37653 SO:0000704 denotes genes
T6857 37664-37670 PR:000000183 denotes TGF-β2
T6858 37718-37722 UBERON:0001037 denotes hair
T6859 37782-37788 PR:000000183 denotes TGF-β2
T6860 37810-37820 GO:0065007 denotes regulating
T6861 37821-37826 PR:000015308 denotes Snail
T6862 37827-37837 GO:0010467 denotes expression
T6863 37841-37854 CL:0000312 denotes keratinocytes
T6864 37873-37887 _FRAGMENT denotes basal layer of
T6865 37892-37901 UBERON:0002025 denotes epidermis
T6866 37996-38009 CL:0000312 denotes keratinocytes
T6867 38101-38111 UBERON:0000483 denotes epithelial
T6868 38101-38117 CL:0000066 denotes epithelial cells
T6869 38171-38184 CL:0000312 denotes keratinocytes
T6870 38214-38220 PR:000000183 denotes TGF-β2
T6871 38263-38268 PR:000015308 denotes Snail
T6872 38308-38313 PR:000015308 denotes Snail
T6873 38412-38417 PR:000015308 denotes Snail
T6874 38503-38509 PR:000000183 denotes TGF-β2
T6875 38609-38614 PR:000000046 denotes TGF-β
T6876 38755-38763 CHEBI:35224 denotes effector
T6877 38764-38769 PR:000000364 denotes SMAD2
T6878 38771-38776 PR:000015308 denotes Snail
T6879 38777-38787 GO:0010467 denotes expression
T6880 38840-38845 PR:000015308 denotes Snail
T6881 38846-38856 GO:0010467 denotes expression
T6882 38888-38893 PR:000000364 denotes SMAD2
T6883 38995-39000 PR:000015308 denotes Snail
T6884 39001-39011 GO:0010467 denotes expression
T6885 39062-39067 PR:000015308 denotes Snail
T6886 39068-39076 SO:0000167 denotes promoter
T6887 39089-39102 CL:0000312 denotes keratinocytes
T6888 39125-39131 PR:000000183 denotes TGF-β2
T6889 39197-39206 SO:0000902 denotes transgene
T6890 39254-39261 GO:0065007 denotes control
T6891 39289-39297 SO:0000167 denotes promoter
T6892 39327-39356 SO:0000315 denotes transcription initiation site
T6893 39352-39359 _FRAGMENT denotes site of
T6894 39376-39380 SO:0000842 denotes gene
T6895 39364-39369 NCBITaxon:10088 denotes mouse
T6896 39370-39375 PR:000015308 denotes Snail
T6897 39405-39417 GO:0009294 denotes transfection
T6898 39419-39432 CL:0000312 denotes keratinocytes
T6899 39451-39457 PR:000000183 denotes TGF-β2
T6900 39487-39496 SO:0000902 denotes transgene
T6901 39557-39562 PR:000015308 denotes Snail
T6902 39657-39663 PR:000000183 denotes TGF-β2
T6903 39675-39680 PR:000015308 denotes Snail
T6904 39681-39689 SO:0000167 denotes promoter
T6905 39813-39818 PR:000015308 denotes Snail
T6906 39819-39827 SO:0000167 denotes promoter
T6907 39895-39908 CL:0000312 denotes keratinocytes
T6908 39926-39931 PR:000015308 denotes Snail
T6909 39932-39940 SO:0000167 denotes promoter
T6910 39988-39993 PR:000015308 denotes Snail
T6911 40074-40080 PR:000000183 denotes TGF-β2
T6912 40143-40149 PR:000000183 denotes TGF-β2
T6913 40192-40207 SO:0000409 denotes binding element
T6914 40241-40246 PR:000015308 denotes Snail
T6915 40247-40271 SO:0000315 denotes transcription start site
T6916 40333-40346 CL:0000312 denotes keratinocytes
T6917 40348-40354 PR:000000183 denotes TGF-β2
T6918 40423-40428 PR:000015308 denotes Snail
T6919 40429-40433 SO:0000704 denotes gene
T6920 40459-40464 PR:000015308 denotes Snail
T6921 40505-40513 SO:0000167 denotes promoter
T6922 40539-40544 PR:000015308 denotes Snail
T6923 40545-40549 SO:0000704 denotes gene
T6924 40575-40581 PR:000000183 denotes TGF-β2
T6925 40599-40612 CL:0000312 denotes keratinocytes
T6926 40650-40655 PR:000015308 denotes Snail
T6927 40694-40707 UBERON:0002073 denotes hair follicle
T6928 40694-40721 GO:0031069 denotes hair follicle morphogenesis
T6929 40725-40734 UBERON:0000922 denotes embryonic
T6930 40735-40740 NCBITaxon:10088 denotes mouse
T6931 40763-40769 PR:000000183 denotes TGF-β2
T6932 40792-40797 PR:000015308 denotes Snail
T6933 40811-40815 UBERON:0001037 denotes hair
T6934 40865-40871 PR:000000183 denotes TGF-β2
T6935 40876-40885 GO:0010467 denotes expressed
T6936 40903-40907 UBERON:0001037 denotes hair
T6937 40965-40971 PR:000000183 denotes TGF-β2
T6938 40972-40980 GO:0042571 denotes antibody
T6939 41000-41004 UBERON:0001037 denotes hair
T6940 41117-41121 NCBITaxon:10088 denotes mice
T6941 41139-41145 PR:000000183 denotes TGF-β2
T6942 41204-41212 CHEBI:35224 denotes effector
T6943 41216-41222 PR:000000183 denotes TGF-β2
T6944 41251-41260 GO:0010467 denotes expressed
T6945 41276-41282 PR:000000183 denotes TGF-β2
T6946 41289-41293 UBERON:0001037 denotes hair
T6947 41366-41372 PR:000000183 denotes TGF-β2
T6948 41373-41380 SO:0001060 denotes isoform
T6949 41389-41399 GO:0010467 denotes expression
T6950 41408-41414 PR:000000182 denotes TGF-β1
T6951 41419-41425 PR:000000183 denotes TGF-β2
T6952 41440-41444 UBERON:0001037 denotes hair
T6953 42509-42514 PR:000015308 denotes Snail
T6954 42519-42525 PR:000000183 denotes TGF-β2
T6955 42553-42558 PR:000015308 denotes Snail
T6956 42559-42569 GO:0010467 denotes expression
T6957 42573-42579 PR:000000183 denotes TGF-β2
T6958 42585-42589 UBERON:0001037 denotes hair
T6959 42631-42636 PR:000015308 denotes Snail
T6960 42675-42681 PR:000000183 denotes TGF-β2
T6961 42687-42694 UBERON:0000922 denotes embryos
T6962 42820-42825 PR:000015308 denotes Snail
T6963 42855-42861 PR:000000183 denotes TGF-β2
T6964 42867-42871 UBERON:0001037 denotes hair
T6965 42942-42946 UBERON:0001037 denotes hair
T6966 43008-43011 PR:000009454 denotes K14
T6967 43012-43017 PR:000000364 denotes Smad2
T6968 43021-43025 NCBITaxon:10088 denotes mice
T6969 43050-43055 PR:000000046 denotes TGF-β
T6970 43050-43065 GO:0007179 denotes TGF-β signaling
T6971 43080-43085 PR:000015308 denotes Snail
T6972 43171-43176 GO:0007567 denotes natal
T6973 43258-43264 PR:000000183 denotes TGF-β2
T6974 43304-43309 PR:000015308 denotes Snail
T6975 43310-43314 SO:0000704 denotes gene
T6976 43310-43325 GO:0010467 denotes gene expression
T6977 43368-43372 UBERON:0001037 denotes hair
T6978 43422-43427 PR:000015308 denotes Snail
T6979 43497-43501 UBERON:0001037 denotes hair
T6980 43523-43529 PR:000000183 denotes TGF-β2
T6981 43632-43638 PR:000000183 denotes TGF-β2
T6982 43678-43688 UBERON:0000483 denotes epithelial
T6983 43746-43759 GO:0009653 denotes morphogenesis
T6984 43843-43849 PR:000000183 denotes TGF-β2
T6985 43898-43908 GO:0042571 denotes antibodies
T6986 43917-43928 PR:000009140 denotes β4 integrin
T6987 43955-43967 CL:0000312 denotes keratinocyte
T6988 44048-44054 PR:000000183 denotes TGF-β2
T6989 44114-44127 GO:0008283 denotes proliferation
T6990 44158-44162 UBERON:0001037 denotes hair
T6991 44187-44191 PR:000010425 denotes Ki67
T6992 44249-44255 PR:000000183 denotes TGF-β2
T6993 44267-44272 PR:000015308 denotes Snail
T6994 44273-44283 GO:0010467 denotes expression
T6995 44359-44369 GO:0010467 denotes expression
T6996 44373-44378 SO:0000704 denotes genes
T6997 44411-44416 PR:000015308 denotes Snail
T6998 44443-44448 PR:000015308 denotes Snail
T6999 44476-44486 PR:000001447 denotes E-cadherin
T7000 44487-44491 SO:0000704 denotes gene
T7001 44531-44541 PR:000001447 denotes E-cadherin
T7002 44545-44551 PR:000000183 denotes TGF-β2
T7003 44586-44590 UBERON:0001037 denotes hair
T7004 44599-44605 PR:000000183 denotes TGF-β2
T7005 44758-44771 GO:0005576 denotes extracellular
T7006 44788-44794 PR:000000021 denotes noggin
T7007 44816-44829 _FRAGMENT denotes generation of
T7008 44862-44869 GO:0065003 denotes complex
T7009 44832-44837 PR:000009751 denotes LEF-1
T7010 44838-44847 PR:000002198 denotes β-catenin
T7011 44862-44869 GO:0032991 denotes complex
T7012 44881-44891 PR:000001447 denotes E-cadherin
T7013 44922-44926 UBERON:0001037 denotes hair
T7014 44989-44995 PR:000000183 denotes TGF-β2
T7015 45016-45023 GO:0005634 denotes nuclear
T7016 45024-45029 PR:000009751 denotes LEF-1
T7017 45034-45043 PR:000002198 denotes β-catenin
T7018 45073-45076 _FRAGMENT denotes Wnt
T7019 45084-45101 GO:0016055 denotes signaling pathway
T7020 45077-45083 PR:000000021 denotes noggin
T9069 45500-45513 GO:0009653 denotes morphogenesis
T9070 45556-45566 UBERON:0000483 denotes epithelium
T9071 45571-45581 UBERON:0003104 denotes mesenchyme
T9072 45582-45588 GO:0065007 denotes govern
T9073 45693-45700 GO:0005634 denotes nuclear
T9074 45705-45714 GO:0005829 denotes cytosolic
T9075 45781-45786 UBERON:0000062 denotes organ
T9076 45781-45800 GO:0009887 denotes organ morphogenesis
T9077 46066-46074 _FRAGMENT denotes cellular
T9078 46087-46100 GO:0008283 denotes proliferation
T9079 46106-46121 GO:0030154 denotes differentiation
T9080 46208-46212 UBERON:0001037 denotes hair
T9319 46271-46284 UBERON:0002073 denotes Hair Follicle
T9320 46271-46298 GO:0031069 denotes Hair Follicle Morphogenesis
T9321 46317-46321 UBERON:0001037 denotes hair
T9322 46326-46339 GO:0009653 denotes morphogenesis
T9323 46381-46391 UBERON:0000483 denotes epithelium
T9324 46396-46399 PR:000000034 denotes BMP
T9325 46439-46449 UBERON:0003104 denotes mesenchyme
T9326 46480-46508 GO:0005667 denotes transcription factor complex
T9327 46519-46528 PR:000002198 denotes β-catenin
T9328 46533-46538 PR:000009751 denotes LEF-1
T9329 46589-46602 UBERON:0002073 denotes hair follicle
T9330 46625-46639 PR:000014841 denotes Sonic hedgehog
T9331 46641-46644 PR:000014841 denotes Shh
T9332 46650-46656 PR:000000183 denotes TGF-β2
T9333 46705-46718 GO:0009653 denotes morphogenesis
T9334 46739-46748 PR:000002198 denotes β-catenin
T9335 46814-46818 UBERON:0001037 denotes hair
T9336 46853-46858 PR:000009751 denotes LEF-1
T9337 46860-46863 PR:000014841 denotes Shh
T9338 46868-46874 PR:000000183 denotes TGF-β2
T9339 46939-46949 UBERON:0010512 denotes guard hair
T9340 46951-46964 GO:0009653 denotes morphogenesis
T9341 47019-47024 UBERON:0001037 denotes hairs
T9342 47047-47065 GO:0065007 denotes regulatory control
T9343 47067-47078 UBERON:0010512 denotes Guard hairs
T9344 47102-47107 PR:000009751 denotes LEF-1
T9345 47112-47118 PR:000000183 denotes TGF-β2
T9346 47161-47168 GO:0010467 denotes express
T9347 47169-47174 PR:000015308 denotes Snail
T9348 47235-47245 PR:000001447 denotes E-cadherin
T9349 47249-47258 GO:0065007 denotes regulated
T9350 47262-47273 UBERON:0010512 denotes guard hairs
T9351 47333-47338 PR:000015308 denotes Snail
T9352 47362-47366 PR:000015309 denotes Slug
T9353 47370-47375 PR:000027825 denotes twist
T9354 47427-47436 PR:000002198 denotes β-catenin
T9355 47467-47472 PR:000009751 denotes LEF-1
T9356 47477-47480 PR:000015643 denotes Sry
T9357 47677-47683 PR:000000046 denotes TGF-βs
T9358 47719-47732 CL:0000312 denotes keratinocytes
T9359 47742-47752 GO:0007049 denotes cell cycle
T9360 47771-47777 PR:000000183 denotes TGF-β2
T9361 47829-47836 GO:0040007 denotes growing
T9362 47853-47862 _FRAGMENT denotes phases of
T9363 47873-47883 GO:0044851 denotes hair cycle
T9364 47867-47872 UBERON:0007023 denotes adult
T9365 47873-47877 UBERON:0001037 denotes hair
T9366 48000-48009 GO:0006915 denotes apoptosis
T9367 48065-48071 PR:000000182 denotes TGF-β1
T9368 48110-48117 GO:0040007 denotes growing
T9369 48131-48136 GO:0007567 denotes natal
T9370 48137-48151 UBERON:0002073 denotes hair follicles
T9371 48153-48159 PR:000000183 denotes TGF-β2
T9372 48182-48191 UBERON:0000922 denotes embryonic
T9373 48275-48281 PR:000000183 denotes TGF-β2
T9374 48284-48293 UBERON:0000922 denotes embryonic
T9375 48294-48304 GO:0010467 denotes expression
T9376 48333-48339 PR:000000183 denotes TGF-β2
T9377 48495-48501 PR:000000046 denotes TGF-βs
T9378 48520-48526 PR:000000183 denotes TGF-β2
T9379 48544-48548 PR:000010425 denotes Ki67
T9380 48549-48559 GO:0010467 denotes expression
T9381 48564-48579 GO:0000187 denotes MAPK activation
T9382 48621-48634 UBERON:0002073 denotes hair follicle
T9383 48635-48648 CL:0000312 denotes keratinocytes
T9384 48693-48699 PR:000000183 denotes TGF-β2
T9385 48763-48776 PR:000000046 denotes TGF-β factors
T9386 49025-49030 SO:0000704 denotes genes
T9387 49072-49078 PR:000000183 denotes TGF-β2
T9388 49082-49095 GO:0008283 denotes proliferation
T9389 49107-49111 UBERON:0001037 denotes hair
T9390 49271-49284 CL:0000312 denotes keratinocytes
T9391 49329-49334 PR:000015308 denotes Snail
T9392 49335-49339 SO:0000704 denotes gene
T9393 49365-49370 PR:000000046 denotes TGF-β
T9394 49365-49380 GO:0007179 denotes TGF-β signaling
T9395 49429-49434 PR:000015308 denotes Snail
T9396 49435-49439 SO:0000704 denotes gene
T9397 49435-49450 GO:0010467 denotes gene expression
T9398 49481-49485 UBERON:0001037 denotes hair
T9399 49526-49536 UBERON:0000483 denotes epithelial
T9400 49542-49555 GO:0008283 denotes proliferation
T9401 49560-49565 PR:000015308 denotes Snail
T9402 49566-49575 SO:0000902 denotes transgene
T9403 49576-49586 GO:0010467 denotes expression
T9404 49644-49650 PR:000000183 denotes TGF-β2
T9405 49655-49660 PR:000015308 denotes Snail
T9406 49710-49716 PR:000000183 denotes TGF-β2
T9407 49718-49723 PR:000015308 denotes Snail
T9408 49724-49734 GO:0010467 denotes expression
T9409 49762-49766 UBERON:0001037 denotes hair
T9410 49792-49795 PR:000009454 denotes K14
T9411 49796-49801 PR:000000364 denotes Smad2
T9412 49808-49813 PR:000015308 denotes Snail
T9413 49900-49906 PR:000000183 denotes TGF-β2
T9414 49956-49961 PR:000015308 denotes Snail
T9415 50005-50010 PR:000015308 denotes Snail
T9416 50025-50034 GO:0010467 denotes expressed
T9417 50042-50055 UBERON:0002073 denotes hair follicle
T9418 50042-50069 GO:0031069 denotes hair follicle morphogenesis
T9419 50126-50136 GO:0010467 denotes expression
T9420 50143-50148 PR:000015308 denotes Snail
T9421 50156-50161 GO:0007567 denotes natal
T9422 50218-50228 UBERON:0000483 denotes epithelial
T9423 50218-50255 GO:0001837 denotes epithelial to mesenchymal transitions
T9424 50232-50243 UBERON:0003104 denotes mesenchymal
T9425 50303-50308 PR:000015308 denotes Snail
T9426 50309-50319 GO:0010467 denotes expression
T9427 50354-50367 UBERON:0002073 denotes hair follicle
T9428 50354-50381 GO:0031069 denotes hair follicle morphogenesis
T9429 50421-50430 UBERON:0010166 denotes hair coat
T9430 50434-50437 PR:000009454 denotes K14
T9431 50438-50443 PR:000015308 denotes Snail
T9432 50447-50451 NCBITaxon:10088 denotes mice
T9433 50571-50576 UBERON:0001037 denotes hairs
T9434 50610-50619 UBERON:0007376 denotes epidermis
T9435 50706-50710 NCBITaxon:10088 denotes mice
T9436 50738-50741 PR:000009454 denotes K14
T9437 50742-50750 SO:0000167 denotes promoter
T9438 50777-50791 _FRAGMENT denotes basal layer of
T9439 50796-50805 UBERON:0002025 denotes epidermis
T9440 50814-50831 _FRAGMENT denotes outer root sheath
T9441 50838-50840 _FRAGMENT denotes of
T9442 50845-50858 UBERON:0005942 denotes hair follicle
T9443 50833-50836 _FRAGMENT denotes ORS
T9444 50905-50914 UBERON:0000922 denotes embryonic
T9445 50915-50919 UBERON:0001037 denotes hair
T9446 50947-50952 GO:0007567 denotes natal
T9447 50953-50957 UBERON:0001037 denotes hair
T9448 50980-50983 PR:000009454 denotes K14
T9449 50984-50992 SO:0000167 denotes promoter
T9450 51020-51023 UBERON:0005942 denotes ORS
T9451 51029-51038 UBERON:0007376 denotes epidermis
T9452 51114-51117 UBERON:0005942 denotes ORS
T9453 51129-51134 PR:000015308 denotes Snail
T9454 51197-51202 PR:000015308 denotes Snail
T9455 51241-51251 GO:0010467 denotes expression
T9456 51289-51299 GO:0065007 denotes regulatory
T9457 51316-51322 GO:0065007 denotes govern
T9458 51323-51325 GO:0005912 denotes AJ
T9459 51323-51335 GO:0034333 denotes AJ formation
T9460 51352-51365 GO:0008283 denotes proliferation
T9461 51373-51385 UBERON:0005942 denotes follicle ORS
T9462 51459-51463 NCBITaxon:10088 denotes mice
T9463 51505-51510 PR:000015308 denotes Snail
T10792 51574-51584 UBERON:0000483 denotes Epithelial
T10793 51601-51614 GO:0008283 denotes Proliferation
T10794 51622-51626 UBERON:0001037 denotes Hair
T10795 51675-51688 UBERON:0002073 denotes hair follicle
T10796 51675-51702 GO:0031069 denotes hair follicle morphogenesis
T10797 51704-51714 PR:000001447 denotes E-cadherin
T10798 51715-51719 SO:0000704 denotes gene
T10799 51715-51730 GO:0010467 denotes gene expression
T10800 51897-51902 PR:000009751 denotes LEF-1
T10801 51903-51912 PR:000002198 denotes β-catenin
T10802 51913-51941 GO:0005667 denotes transcription factor complex
T10803 51996-52001 PR:000009751 denotes LEF-1
T10804 52006-52018 SO:0000409 denotes binding site
T10805 52026-52036 PR:000001447 denotes E-cadherin
T10806 52037-52045 SO:0000167 denotes promoter
T10807 52122-52127 PR:000009751 denotes LEF-1
T10808 52128-52137 PR:000002198 denotes β-catenin
T10809 52170-52180 PR:000001447 denotes E-cadherin
T10810 52181-52185 SO:0000704 denotes gene
T10811 52181-52196 GO:0010467 denotes gene expression
T10812 52205-52218 CL:0000312 denotes keratinocytes
T10813 52275-52280 PR:000015308 denotes Snail
T10814 52320-52333 CL:0000312 denotes keratinocytes
T10815 52382-52387 PR:000015308 denotes Snail
T10816 52391-52400 GO:0010467 denotes expressed
T10817 52479-52485 PR:000000021 denotes noggin
T10818 52491-52500 UBERON:0000922 denotes embryonic
T10819 52507-52512 PR:000009751 denotes LEF-1
T10820 52513-52523 GO:0010467 denotes expression
T10821 52557-52562 PR:000009751 denotes LEF-1
T10822 52563-52572 PR:000002198 denotes β-catenin
T10823 52582-52586 SO:0000704 denotes gene
T10824 52657-52667 PR:000001447 denotes E-cadherin
T10825 52714-52717 _FRAGMENT denotes Wnt
T10826 52725-52734 GO:0016055 denotes signaling
T10827 52718-52724 PR:000000021 denotes noggin
T10828 52738-52748 GO:0065007 denotes regulating
T10829 52772-52785 GO:0009653 denotes morphogenesis
T10830 52803-52807 SO:0000704 denotes gene
T10831 52865-52870 PR:000015308 denotes Snail
T10832 52909-52927 _FRAGMENT denotes down-regulation in
T10833 52939-52954 GO:0010629 denotes gene expression
T10834 52928-52938 PR:000001447 denotes E-cadherin
T10835 52939-52943 SO:0000704 denotes gene
T10836 53025-53028 PR:000009454 denotes K14
T10837 53029-53034 PR:000015308 denotes Snail
T10838 53038-53047 UBERON:0007376 denotes epidermis
T10839 53067-53085 _FRAGMENT denotes down-regulation in
T10840 53097-53107 GO:0010629 denotes expression
T10841 53086-53096 PR:000001447 denotes E-cadherin
T10842 53207-53217 PR:000001447 denotes E-cadherin
T10843 53265-53278 UBERON:0002073 denotes hair follicle
T10844 53265-53292 GO:0031069 denotes hair follicle morphogenesis
T10845 53327-53336 UBERON:0007376 denotes epidermal
T10846 53342-53355 GO:0008283 denotes proliferation
T10847 53368-53371 PR:000009454 denotes K14
T10848 53372-53377 PR:000015308 denotes Snail
T10849 53428-53441 GO:0008283 denotes proliferation
T10850 53446-53451 PR:000015308 denotes Snail
T10851 53455-53461 PR:000000183 denotes TGF-β2
T10852 53467-53471 UBERON:0001037 denotes hair
T10853 53525-53535 PR:000001447 denotes E-cadherin
T10854 53552-53565 GO:0009653 denotes morphogenesis
T10855 53706-53716 GO:0065007 denotes regulation
T10856 53720-53730 PR:000001447 denotes E-cadherin
T10857 53732-53737 PR:000015308 denotes Snail
T10858 53804-53806 GO:0005912 denotes AJ
T10859 53855-53860 PR:000009231 denotes Ajuba
T10860 53923-53928 PR:000008224 denotes Grb-2
T10861 53992-54005 CL:0000312 denotes keratinocytes
T10862 54078-54085 GO:0010467 denotes express
T10863 54086-54091 PR:000015308 denotes Snail
T10864 54093-54098 PR:000009231 denotes Ajuba
T10865 54128-54133 PR:000008224 denotes Grb-2
T10866 54171-54184 CL:0000312 denotes keratinocytes
T10867 54224-54229 PR:000015308 denotes Snail
T10868 54230-54241 GO:0009294 denotes transfected
T10869 54245-54250 PR:000009231 denotes Ajuba
T10870 54251-54262 GO:0009294 denotes transfected
T10871 54263-54276 CL:0000312 denotes keratinocytes
T10872 54349-54354 PR:000008224 denotes Grb-2
T10873 54370-54375 PR:000009231 denotes Ajuba
T10874 54414-54416 GO:0005912 denotes AJ
T10875 54475-54488 GO:0008283 denotes proliferation
T10876 54500-54510 UBERON:0000483 denotes epithelial
T10877 54500-54510 _FRAGMENT denotes epithelial
T10878 54554-54558 CL:0000066 denotes cell
T10879 54530-54536 NCBITaxon:9608 denotes canine
T10880 54537-54543 UBERON:0002113 denotes kidney
T10881 54566-54571 PR:000015308 denotes Snail
T10882 54596-54606 GO:0007049 denotes cell cycle
T10883 54734-54739 PR:000015308 denotes Snail
T10884 54756-54766 UBERON:0000483 denotes epithelial
T10885 54807-54812 PR:000015308 denotes Snail
T10886 54939-54944 PR:000015308 denotes Snail
T10887 54945-54955 GO:0010467 denotes expression
T10888 55030-55035 NCBITaxon:10088 denotes mouse
T10889 55333-55335 GO:0005912 denotes AJ
T10890 55369-55382 GO:0008283 denotes proliferation
T10891 55459-55469 UBERON:0000483 denotes epithelial
T10892 55470-55483 GO:0008283 denotes proliferation
T10893 55508-55510 GO:0005912 denotes AJ
T10894 55833-55846 GO:0008283 denotes proliferation
T10895 55850-55860 UBERON:0000483 denotes epithelial
T10896 55850-55874 GO:0002009 denotes epithelial morphogenesis
T11940 56104-56112 CHEBI:33893 denotes Reagents
T11941 56121-56131 GO:0042571 denotes antibodies
T11942 56151-56161 PR:000001447 denotes E-cadherin
T11943 56213-56222 PR:000002198 denotes β-catenin
T11944 56231-56238 PR:000028799 denotes tubulin
T11945 56284-56289 PR:000009231 denotes Ajuba
T11946 56364-56375 PR:000009140 denotes β4 integrin
T11947 56376-56381 PR:000009140 denotes CD104
T11948 56437-56446 GO:0005610 denotes laminin 5
T11949 56523-56525 PR:000009481 denotes K5
T11950 56527-56529 PR:000009450 denotes K1
T11951 56531-56539 PR:000009882 denotes loricrin
T11952 56553-56563 PR:000009167 denotes involucrin
T11953 56565-56574 PR:000007551 denotes fillagrin
T11954 56693-56698 PR:000008224 denotes Grb-2
T11955 56760-56770 PR:000005245 denotes P-cadherin
T11956 56866-56874 PR:000017298 denotes vimentin
T11957 56924-56928 PR:000010425 denotes Ki67
T11958 57130-57136 PR:000000183 denotes TGF-β2
T11959 57204-57208 CHEBI:37926 denotes FITC
T11960 57211-57220 CHEBI:51247 denotes Texas Red
T11961 57230-57240 MOP:0000779 denotes conjugated
T11962 57251-57261 GO:0042571 denotes antibodies
T11963 57338-57350 MOP:0000093 denotes Biotinylated
T11964 57361-57371 GO:0042571 denotes antibodies
T11965 57502-57507 PR:000015308 denotes Snail
T11966 57508-57516 GO:0042571 denotes antibody
T11967 57534-57545 NCBITaxon:10140 denotes Guinea pigs
T11968 57598-57604 NCBITaxon:39107 denotes murine
T11969 57605-57610 PR:000015308 denotes Snail
T11970 57685-57690 NCBITaxon:9606 denotes human
T11971 57691-57697 PR:000000183 denotes TGF-β2
T11972 57779-57785 PR:000000183 denotes TGF-β2
T65 30-36 PR:000000183 denotes TGF-β2
T7021 45145-45158 UBERON:0002073 denotes hair follicle
T7022 45145-45172 GO:0031069 denotes hair follicle morphogenesis
T7023 45174-45180 PR:000000183 denotes TGF-β2
T7024 45211-45217 PR:000000021 denotes noggin
T7025 45244-45248 UBERON:0001037 denotes hair
T7026 45287-45292 PR:000015308 denotes Snail
T7027 45293-45297 SO:0000704 denotes gene
T7028 45293-45308 GO:0010467 denotes gene expression
T7029 45310-45316 PR:000000183 denotes TGF-β2
T7030 45388-45398 PR:000001447 denotes E-cadherin
R5885 T9363 T9362 _lexicallyChainedTo hair cycle,phases of
R5886 T9439 T9438 _lexicallyChainedTo epidermis,basal layer of
R5887 T9441 T9440 _lexicallyChainedTo of,outer root sheath
R5888 T9441 T9443 _lexicallyChainedTo of,ORS
R5889 T9442 T9441 _lexicallyChainedTo hair follicle,of
R6870 T10840 T10839 _lexicallyChainedTo expression,down-regulation in
R6871 T10878 T10877 _lexicallyChainedTo cell,epithelial
R2287 T3750 T3749 _lexicallyChainedTo spinous,layers
R5768 T9078 T9077 _lexicallyChainedTo proliferation,cellular
R5769 T9079 T9077 _lexicallyChainedTo differentiation,cellular
R6868 T10826 T10825 _lexicallyChainedTo signaling,Wnt
R6869 T10833 T10832 _lexicallyChainedTo gene expression,down-regulation in
R8872 T13611 T13610 _lexicallyChainedTo UTR,region of
R215 T574 T573 _lexicallyChainedTo organs,formation of
R216 T606 T605 _lexicallyChainedTo of,proliferation
R217 T607 T606 _lexicallyChainedTo cells,of
R218 T612 T611 _lexicallyChainedTo cell fates,determination of
R219 T696 T695 _lexicallyChainedTo cells,motile
R1417 T2432 T2431 _lexicallyChainedTo UTR,3′
R1418 T2469 T2468 _lexicallyChainedTo cells,commitment of
R1419 T2470 T2469 _lexicallyChainedTo to,cells
R1420 T2471 T2470 _lexicallyChainedTo cell fate,to
R1421 T2473 T2472 _lexicallyChainedTo ectoderm,cells of
R1422 T2481 T2480 _lexicallyChainedTo follicles,hair
R1423 T2494 T2493 _lexicallyChainedTo cells,nuclei of
R1424 T2524 T2523 _lexicallyChainedTo expression,down-regulation in
R2874 T4839 T4838 _lexicallyChainedTo to,translocation
R2875 T4840 T4839 _lexicallyChainedTo nucleus,to
R4159 T6800 T6799 _lexicallyChainedTo Gene Expression,Regulation of
R4160 T6823 T6822 _lexicallyChainedTo signaling,Wnt
R4161 T6865 T6864 _lexicallyChainedTo epidermis,basal layer of
R4162 T6894 T6893 _lexicallyChainedTo gene,site of
R4163 T7008 T7007 _lexicallyChainedTo complex,generation of
R4164 T7019 T7018 _lexicallyChainedTo signaling pathway,Wnt

2_test

Id Subject Object Predicate Lexical cue
15630473-9988217-84810234 1913-1914 9988217 denotes 1
15630473-11841536-84810235 2671-2672 11841536 denotes 2
15630473-1566372-84810236 4001-4002 1566372 denotes 3
15630473-12646922-84810237 4729-4730 12646922 denotes 4
15630473-12600316-84810238 4994-4995 12600316 denotes 5
15630473-12646922-84810239 5259-5260 12646922 denotes 4
15630473-9722621-84810240 5566-5567 9722621 denotes 6
15630473-10660044-84810241 5568-5569 10660044 denotes 7
15630473-12361600-84810242 5570-5571 12361600 denotes 8
15630473-10330178-84810243 5824-5825 10330178 denotes 9
15630473-12417594-84810244 5826-5828 12417594 denotes 10
15630473-11944044-84810245 6107-6109 11944044 denotes 11
15630473-10655587-84810246 6383-6385 10655587 denotes 12
15630473-10655586-84810247 6386-6388 10655586 denotes 13
15630473-12508111-84810248 6389-6391 12508111 denotes 14
15630473-12665527-84810249 6392-6394 12665527 denotes 15
15630473-11994736-84810250 6624-6626 11994736 denotes 16
15630473-12189386-84810251 6627-6629 12189386 denotes 17
15630473-12646922-84810252 7032-7033 12646922 denotes 4
15630473-14644200-84810253 8717-8719 14644200 denotes 18
15630473-12646922-84810254 9060-9061 12646922 denotes 4
15630473-12646922-84810255 9157-9158 12646922 denotes 4
15630473-7958926-84810256 9159-9161 7958926 denotes 19
15630473-10655587-84810257 14578-14580 10655587 denotes 12
15630473-10655586-84810258 14581-14583 10655586 denotes 13
15630473-10498690-84810259 15526-15528 10498690 denotes 20
15630473-12646922-84810260 15638-15639 12646922 denotes 4
15630473-10655587-84810261 16096-16098 10655587 denotes 12
15630473-11689706-84810262 19815-19817 11689706 denotes 21
15630473-12646922-84810263 23133-23134 12646922 denotes 4
15630473-10655587-84810264 23135-23137 10655587 denotes 12
15630473-10655586-84810265 23138-23140 10655586 denotes 13
15630473-10660044-84810266 26676-26677 10660044 denotes 7
15630473-11062015-84810267 29258-29260 11062015 denotes 22
15630473-10542129-84810268 30747-30749 10542129 denotes 23
15630473-11564756-84810269 30750-30752 11564756 denotes 24
15630473-11381089-84810270 30753-30755 11381089 denotes 25
15630473-10201372-84810271 30959-30961 10201372 denotes 26
15630473-10498690-84810272 31096-31098 10498690 denotes 20
15630473-12417594-84810273 31372-31374 12417594 denotes 10
15630473-10330178-84810274 31561-31562 10330178 denotes 9
15630473-7527391-84810275 33033-33035 7527391 denotes 27
15630473-10330178-84810276 33128-33129 10330178 denotes 9
15630473-11841536-84810277 34370-34371 11841536 denotes 2
15630473-11994736-84810278 34372-34374 11994736 denotes 16
15630473-7626420-84810279 34375-34377 7626420 denotes 28
15630473-12646922-84810280 34803-34804 12646922 denotes 4
15630473-12646922-84810281 34938-34939 12646922 denotes 4
15630473-12646922-84810282 35103-35104 12646922 denotes 4
15630473-10559902-84810283 35105-35107 10559902 denotes 29
15630473-11371349-84810284 35108-35110 11371349 denotes 30
15630473-12646922-84810285 35644-35645 12646922 denotes 4
15630473-12665527-84810286 37415-37417 12665527 denotes 15
15630473-11017879-84810287 37419-37421 11017879 denotes 31
15630473-10433821-84810288 37578-37580 10433821 denotes 32
15630473-10433821-84810289 37734-37736 10433821 denotes 32
15630473-1955457-84810290 40952-40954 1955457 denotes 33
15630473-9217007-84810291 41173-41175 9217007 denotes 34
15630473-1955457-84810292 41749-41751 1955457 denotes 33
15630473-11456453-84810293 42132-42134 11456453 denotes 35
15630473-11456453-84810294 43075-43077 11456453 denotes 35
15630473-12665527-84810295 43478-43480 12665527 denotes 15
15630473-10433821-84810296 43588-43590 10433821 denotes 32
15630473-10655587-84810297 44493-44495 10655587 denotes 12
15630473-10655586-84810298 44496-44498 10655586 denotes 13
15630473-12646922-84810299 46609-46610 12646922 denotes 4
15630473-10559902-84810300 46611-46613 10559902 denotes 29
15630473-11371349-84810301 46614-46616 11371349 denotes 30
15630473-9845363-84810302 46617-46619 9845363 denotes 36
15630473-12015971-84810303 46620-46622 12015971 denotes 37
15630473-11371349-84810304 46804-46806 11371349 denotes 30
15630473-10433821-84810305 46876-46878 10433821 denotes 32
15630473-9768360-84810306 46879-46881 9768360 denotes 38
15630473-15210113-84810307 47529-47531 15210113 denotes 40
15630473-12809600-84810308 47754-47756 12809600 denotes 41
15630473-14708594-84810309 48012-48014 14708594 denotes 42
15630473-10433821-84810310 48227-48229 10433821 denotes 32
15630473-1955457-84810311 48315-48317 1955457 denotes 33
15630473-9684281-84810312 49227-49229 9684281 denotes 43
15630473-7685120-84810313 49286-49288 7685120 denotes 44
15630473-8732677-84810314 49289-49291 8732677 denotes 45
15630473-9122204-84810315 49292-49294 9122204 denotes 46
15630473-12665527-84810316 49382-49384 12665527 denotes 15
15630473-14644200-84810317 50383-50385 14644200 denotes 18
15630473-12646922-84810318 51824-51825 12646922 denotes 4
15630473-10498690-84810319 51943-51945 10498690 denotes 20
15630473-12646922-84810320 52220-52221 12646922 denotes 4
15630473-12646922-84810321 52787-52788 12646922 denotes 4
15630473-12646922-84810322 53310-53311 12646922 denotes 4
15630473-10660044-84810323 53312-53313 10660044 denotes 7
15630473-10330178-84810324 53951-53952 10330178 denotes 9
15630473-12417594-84810325 53953-53955 12417594 denotes 10
15630473-15155580-84810326 54672-54674 15155580 denotes 47
15630473-10660044-84810327 55521-55522 10660044 denotes 7
15630473-10542129-84810328 55523-55525 10542129 denotes 23
15630473-11564756-84810329 55526-55528 11564756 denotes 24
15630473-14623871-84810330 55529-55531 14623871 denotes 48
15630473-10411913-84810331 58061-58063 10411913 denotes 49
15630473-9217007-84810332 58254-58256 9217007 denotes 34
15630473-9768360-84810333 58277-58279 9768360 denotes 38
15630473-10498690-84810334 58299-58301 10498690 denotes 20
15630473-12646922-84810335 59937-59938 12646922 denotes 4
15630473-12646922-84810336 60852-60853 12646922 denotes 4
15630473-10330178-84810337 62189-62190 10330178 denotes 9
T56986 1913-1914 9988217 denotes 1
T26441 2671-2672 11841536 denotes 2
T35543 4001-4002 1566372 denotes 3
T8676 4729-4730 12646922 denotes 4
T8006 4994-4995 12600316 denotes 5
T43029 5259-5260 12646922 denotes 4
T83519 5566-5567 9722621 denotes 6
T99899 5568-5569 10660044 denotes 7
T86430 5570-5571 12361600 denotes 8
T24011 5824-5825 10330178 denotes 9
T57385 5826-5828 12417594 denotes 10
T38439 6107-6109 11944044 denotes 11
T86626 6383-6385 10655587 denotes 12
T40333 6386-6388 10655586 denotes 13
T36104 6389-6391 12508111 denotes 14
T598 6392-6394 12665527 denotes 15
T66466 6624-6626 11994736 denotes 16
T44343 6627-6629 12189386 denotes 17
T77084 7032-7033 12646922 denotes 4
T89400 8717-8719 14644200 denotes 18
T54398 9060-9061 12646922 denotes 4
T55026 9157-9158 12646922 denotes 4
T82528 9159-9161 7958926 denotes 19
T99055 14578-14580 10655587 denotes 12
T23817 14581-14583 10655586 denotes 13
T93454 15526-15528 10498690 denotes 20
T72430 15638-15639 12646922 denotes 4
T65812 16096-16098 10655587 denotes 12
T54526 19815-19817 11689706 denotes 21
T84644 23133-23134 12646922 denotes 4
T72423 23135-23137 10655587 denotes 12
T34693 23138-23140 10655586 denotes 13
T30922 26676-26677 10660044 denotes 7
T81771 29258-29260 11062015 denotes 22
T68612 30747-30749 10542129 denotes 23
T96348 30750-30752 11564756 denotes 24
T2510 30753-30755 11381089 denotes 25
T95501 30959-30961 10201372 denotes 26
T10879 31096-31098 10498690 denotes 20
T22135 31372-31374 12417594 denotes 10
T49386 31561-31562 10330178 denotes 9
T43778 33033-33035 7527391 denotes 27
T81408 33128-33129 10330178 denotes 9
T39921 34370-34371 11841536 denotes 2
T6199 34372-34374 11994736 denotes 16
T84568 34375-34377 7626420 denotes 28
T5343 34803-34804 12646922 denotes 4
T23589 34938-34939 12646922 denotes 4
T72158 35103-35104 12646922 denotes 4
T26019 35105-35107 10559902 denotes 29
T73597 35108-35110 11371349 denotes 30
T81547 35644-35645 12646922 denotes 4
T97701 37415-37417 12665527 denotes 15
T28640 37419-37421 11017879 denotes 31
T33595 37578-37580 10433821 denotes 32
T50094 37734-37736 10433821 denotes 32
T16047 40952-40954 1955457 denotes 33
T66515 41173-41175 9217007 denotes 34
T63420 41749-41751 1955457 denotes 33
T83640 42132-42134 11456453 denotes 35
T29049 43075-43077 11456453 denotes 35
T92403 43478-43480 12665527 denotes 15
T88967 43588-43590 10433821 denotes 32
T40416 44493-44495 10655587 denotes 12
T34163 44496-44498 10655586 denotes 13
T58164 46609-46610 12646922 denotes 4
T54633 46611-46613 10559902 denotes 29
T83144 46614-46616 11371349 denotes 30
T56620 46617-46619 9845363 denotes 36
T69133 46620-46622 12015971 denotes 37
T61236 46804-46806 11371349 denotes 30
T21749 46876-46878 10433821 denotes 32
T2540 46879-46881 9768360 denotes 38
T30495 47529-47531 15210113 denotes 40
T44318 47754-47756 12809600 denotes 41
T56843 48012-48014 14708594 denotes 42
T14593 48227-48229 10433821 denotes 32
T83401 48315-48317 1955457 denotes 33
T22810 49227-49229 9684281 denotes 43
T51175 49286-49288 7685120 denotes 44
T29304 49289-49291 8732677 denotes 45
T58249 49292-49294 9122204 denotes 46
T8352 49382-49384 12665527 denotes 15
T28228 50383-50385 14644200 denotes 18
T15084 51824-51825 12646922 denotes 4
T49798 51943-51945 10498690 denotes 20
T78831 52220-52221 12646922 denotes 4
T97560 52787-52788 12646922 denotes 4
T76971 53310-53311 12646922 denotes 4
T78287 53312-53313 10660044 denotes 7
T51345 53951-53952 10330178 denotes 9
T41075 53953-53955 12417594 denotes 10
T85116 54672-54674 15155580 denotes 47
T7746 55521-55522 10660044 denotes 7
T26662 55523-55525 10542129 denotes 23
T19066 55526-55528 11564756 denotes 24
T22662 55529-55531 14623871 denotes 48
T29636 58061-58063 10411913 denotes 49
T80996 58254-58256 9217007 denotes 34
T72165 58277-58279 9768360 denotes 38
T59270 58299-58301 10498690 denotes 20
T55664 59937-59938 12646922 denotes 4
T80769 60852-60853 12646922 denotes 4
T59433 62189-62190 10330178 denotes 9

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T12440 57858-57862 NCBITaxon:10088 denotes Mice
T12441 57867-57870 PR_EXT:000009454 denotes K14
T12442 57871-57876 PR_EXT:000015308 denotes Snail
T12443 57877-57879 SO_EXT:transgenic_entity denotes Tg
T12444 57880-57885 NCBITaxon:10088 denotes mouse
T12445 57927-57932 PR_EXT:000015308 denotes Snail
T12446 57936-57943 SO_EXT:0000155 denotes plasmid
T12447 58011-58016 PR_EXT:P23940 denotes BamHI
T12448 58049-58052 PR_EXT:000009454 denotes K14
T12449 58053-58059 SO_EXT:0000440 denotes vector
T12450 58070-58080 SO_EXT:sequence_linearization denotes linearized
T12451 58081-58090 SO_EXT:engineered_biological_sequence denotes construct
T12452 58113-58120 GO:0005634 denotes nucleus
T12453 58124-58131 UBERON:0000922 denotes embryos
T12454 58141-58145 NCBITaxon:10088 denotes mice
T12455 58151-58154 PR_EXT:000009454 denotes K14
T12456 58155-58161 PR_EXT:000000364 denotes Smad 2
T12457 58162-58164 SO_EXT:transgenic_entity denotes Tg
T12458 58165-58170 NCBITaxon:10088 denotes mouse
T12459 58209-58215 PR_EXT:000000183 denotes TGF-β2
T12460 58230-58235 NCBITaxon:10088 denotes mouse
T12461 58263-58266 PR_EXT:000014841 denotes shh
T12462 58270-58275 NCBITaxon:10088 denotes mouse
T12463 58292-58297 NCBITaxon:10088 denotes mouse
T12647 58372-58379 CHEBI_PR_EXT:protein denotes Protein
T12648 58402-58415 CL:0000312 denotes keratinocytes
T12649 58441-58447 GO:0019835 denotes lysing
T12650 58448-58453 CL_GO_EXT:cell denotes cells
T12651 58457-58462 GO:0019835 denotes lysis
T12652 58463-58469 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T12653 58474-58479 CHEBI:63016 denotes NP-40
T12654 58484-58503 CHEBI:9177 denotes sodium deoxycholate
T12655 58511-58515 CHEBI:9754 denotes Tris
T12656 58516-58518 CHEBI:17996 denotes Cl
T12657 58536-58540 CHEBI:26710 denotes NaCl
T12658 58557-58572 CHEBI:35607 denotes sodium vanadate
T12659 58579-58608 CHEBI:8102 denotes phenylmethylsulfonyl fluoride
T12660 58614-58633 CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor denotes protease inhibitors
T12661 58658-58665 CHEBI_CHMO_EXT:buffer_solution denotes bβuffer
T12662 58682-58686 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T12663 58687-58693 UBERON:0000479 denotes tissue
T12664 58702-58708 UBERON:0000479 denotes tissue
T12665 58736-58744 CHEBI_EXT:nitrogen denotes nitrogen
T12666 58832-58838 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T12667 58843-58855 CHEBI:9750 denotes Triton X-100
T12668 58874-58878 CHEBI_EXT:EDTA denotes EDTA
T12669 58887-58891 CHEBI:26710 denotes NaCl
T12670 58896-58915 CHEBI:9177 denotes sodium deoxycholate
T12671 58926-58929 CHEBI:8984 denotes SDS
T12672 58935-58954 CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor denotes protease inhibitors
T12673 58966-58972 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T12674 58988-58992 CL_GO_EXT:cell denotes cell
T12675 59216-59223 CHEBI_PR_EXT:protein denotes Protein
T12676 59303-59311 GO:0042571 denotes antibody
T12677 59344-59351 CHEBI_PR_EXT:protein denotes Protein
T12678 59481-59486 GO:0019835 denotes lysis
T12679 59487-59493 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T12680 59503-59510 CHEBI_PR_EXT:protein denotes Protein
T12681 59523-59531 GO:0042571 denotes antibody
T12682 59532-59539 CHEBI_EXT:59132 denotes antigen
T12683 59574-59580 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T12684 59624-59627 CHEBI:8984 denotes SDS
T12685 59637-59648 GO_EXT:biological_movement_or_translocation_process denotes transferred
T12686 59652-59666 CHEBI:53325 denotes nitrocellulose
T13019 59842-59846 CL_GO_EXT:cell denotes Cell
T13020 59863-59876 CL:0000312 denotes keratinocytes
T13021 59897-59904 CHEBI_EXT:calcium denotes calcium
T13022 59951-59964 GO:0009294 denotes transfections
T13023 59995-60002 CHEBI_EXT:33893 denotes reagent
T13024 60106-60121 GO_EXT:0004565 denotes β-galactosidase
T13025 60125-60135 GO_EXT:0045289 denotes luciferase
T13026 60146-60154 SO_EXT:0000167 denotes promoter
T13027 60282-60292 GO_EXT:0045289 denotes luciferase
T13028 60353-60360 NCBITaxon:6134 denotes Runella
T13029 60353-60371 GO_EXT:0050248 denotes Runella luciferase
T13030 60378-60389 GO:0009294 denotes transfected
T13031 60395-60400 CL_GO_EXT:cell denotes cells
T13032 60416-60428 GO:0009294 denotes transfection
T13033 60633-60648 GO_PATO_EXT:phosphorylation_process denotes phosphorylation
T13034 60652-60656 GO_PR_EXT:MAP_kinase denotes MAPK
T13035 60658-60671 CL:0000312 denotes keratinocytes
T13036 60677-60682 UBERON:0001977 denotes serum
T13037 60722-60727 CL_GO_EXT:cell denotes cells
T13038 60760-60765 UBERON:0001977 denotes serum
T13039 60780-60785 CL_GO_EXT:cell denotes cells
T13040 60800-60806 PR_EXT:000000021 denotes noggin
T13269 60857-60867 SO_EXT:engineered_biological_sequence denotes Constructs
T13270 60877-60878 CHEBI_SO_EXT:base denotes b
T13271 60879-60885 NCBITaxon:39107 denotes murine
T13272 60886-60891 PR_EXT:000015308 denotes Snail
T13273 60892-60900 SO_EXT:0000167 denotes promoter
T13274 60930-60944 SO_EXT:0000121 denotes forward primer
T13275 60965-60973 SO_EXT:biological_sequence denotes sequence
T13276 61027-61041 SO_EXT:0000132 denotes reverse primer
T13277 61062-61070 SO_EXT:biological_sequence denotes sequence
T13278 61116-61121 NCBITaxon:10088 denotes mouse
T13279 61122-61133 SO_EXT:genomic_DNA denotes genomic DNA
T13280 61130-61133 CHEBI_SO_EXT:DNA denotes DNA
T13281 61153-61164 SO_EXT:0000006 denotes PCR product
T13282 61256-61263 GO:0006266 denotes ligated
T13283 61283-61289 SO_EXT:0000440 denotes vector
T13284 61345-61353 SO_EXT:0000167 denotes promoter
T13285 61440-61443 GO_EXT:0015925 denotes gal
T13286 61450-61456 SO_EXT:0000440 denotes vector
T13287 61526-61541 SO_EXT:point_mutation_entity_or_process denotes point mutations
T13288 61549-61553 GO_PR_EXT:SMAD denotes SMAD
T13289 61554-61561 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T13290 61554-61569 SO_EXT:0000409 denotes binding element
T13291 61669-61683 SO_EXT:0000121 denotes forward primer
T13292 61745-61759 SO_EXT:0000132 denotes reverse primer
T13293 61820-61826 CHEBI_SO_EXT:molecular_probe denotes probes
T13294 61835-61840 PR_EXT:000015308 denotes Snail
T13295 61849-61862 GO:0097617 denotes hybridization
T13296 61890-61896 SO_EXT:0000205 denotes 3′ UTR
T13297 61914-61928 SO_EXT:0000121 denotes forward primer
T13298 61970-61984 SO_EXT:0000132 denotes reverse primer
T13299 62022-62033 SO_EXT:genomic_DNA denotes genomic DNA
T13300 62030-62033 CHEBI_SO_EXT:DNA denotes DNA
T13301 62053-62064 SO_EXT:0000006 denotes PCR product
T13302 62086-62093 GO:0006266 denotes ligated
T13303 62113-62119 SO_EXT:0000440 denotes vector
T13304 62133-62139 SO_EXT:0000417 denotes domain
T13305 62143-62148 PR_EXT:000009231 denotes Ajuba
T13306 62201-62206 SO_EXT:sequence_fusion_process denotes fused
T13307 62249-62255 SO_EXT:0000440 denotes vector
T13626 62291-62304 GO:0097617 denotes hybridization
T13627 62323-62329 SO_EXT:0000440 denotes vector
T13628 62343-62352 _FRAGMENT denotes region of
T13629 62360-62363 SO_EXT:0000837 denotes UTR
T13630 62357-62363 SO_EXT:0000205 denotes 3′ UTR
T13631 62367-62372 PR_EXT:000015308 denotes Snail
T13632 62408-62419 CHEBI:42098 denotes digoxigenin
T13633 62420-62427 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T13634 62438-62447 SO:0000077 denotes antisense
T13635 62448-62458 CHEBI_SO_EXT:molecular_probe denotes riboprobes
T13636 62483-62489 CHEBI_SO_EXT:molecular_probe denotes probes
T13637 62516-62521 PR_EXT:P23940 denotes BamH1
T13638 62542-62556 GO:0097617 denotes hybridizations
T13639 62605-62610 NCBITaxon:10088 denotes mouse
T13640 62611-62618 UBERON:0000922 denotes embryos
T13641 62652-62655 CHEBI_EXT:paraformaldehye denotes PFA
T13642 62691-62701 GO:0097617 denotes hybridized
T13643 62733-62743 GO:0097617 denotes hybridized
T13644 62753-62758 CHEBI_SO_EXT:molecular_probe denotes probe
T13645 62835-62838 CHEBI:42098 denotes dig
T13646 62846-62854 GO:0042571 denotes antibody
T13647 62936-62939 CHEBI:7586 denotes NBT
T13648 62944-62948 CHEBI:75508 denotes BCIP
T13817 63030-63036 UBERON:0000479 denotes Tissue
T13818 63154-63170 CHEBI_EXT:paraformaldehye denotes paraformaldehyde
T13819 63229-63239 GO:0042571 denotes antibodies
T13820 63241-63247 UBERON:0000479 denotes Tissue
T13821 63298-63314 CHEBI_EXT:paraformaldehye denotes paraformaldehyde
T13822 63444-63452 GO:0042571 denotes antibody
T13823 63475-63480 PR_EXT:000015308 denotes Snail
T13824 63501-63509 PR_EXT:cyclin_D denotes cyclin D
T13825 63515-63522 CHEBI_EXT:59132 denotes antigen
T13826 63543-63557 CHEBI:53258 denotes sodium citrate
T13827 63571-63578 CHEBI_EXT:59132 denotes Antigen
T13990 63750-63752 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T13991 63757-63760 CHEBI_SO_EXT:RNA denotes RNA
T13992 63780-63793 CL:0000312 denotes keratinocytes
T13993 63797-63801 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T13994 63802-63808 UBERON:0000479 denotes tissue
T13995 63876-63880 SO_EXT:cDNA denotes cDNA
T13996 63901-63906 CHEBI_SO_EXT:oligonucleotide denotes oligo
T13997 63907-63909 CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group denotes dT
T13998 63910-63917 SO_EXT:0000112 denotes primers
T13999 63963-63970 SO_EXT:0000112 denotes primers
T14000 63989-63994 PR_EXT:000015308 denotes Snail
T14001 63995-64002 SO:0001030 denotes forward
T14002 64033-64038 PR_EXT:000015308 denotes Snail
T14003 64039-64046 SO:0001031 denotes reverse
T14004 64075-64080 GO_PR_EXT:glyceraldehyde_3_phosphate_dehydrogenase denotes GAPDH
T14005 64081-64088 SO:0001030 denotes forward
T14006 64126-64131 GO_PR_EXT:glyceraldehyde_3_phosphate_dehydrogenase denotes GAPDH
T14007 64132-64139 SO:0001031 denotes reverse
T89 2-19 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes Signaling Pathway
T90 30-36 PR_EXT:000000183 denotes TGF-β2
T91 41-46 PR_EXT:000015308 denotes Snail
T92 50-63 UBERON:0002073 denotes Hair Follicle
T93 50-77 GO:0031069 denotes Hair Follicle Morphogenesis
T94 150-163 GO:0048513 denotes organogenesis
T95 173-178 CL_GO_EXT:cell denotes cells
T96 200-216 UBERON:0010136 denotes epithelial sheet
T97 295-299 UBERON:0001037 denotes hair
T98 304-317 GO:0009653 denotes morphogenesis
T99 345-351 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T100 352-357 NCBITaxon:10088 denotes mouse
T101 378-391 CL:0000312 denotes keratinocytes
T102 433-446 GO:0005576 denotes extracellular
T103 438-446 CL_GO_EXT:cell denotes cellular
T104 469-477 GO_PATO_EXT:biological_adhesion_entity_or_process denotes adhesion
T105 491-504 GO:0008283 denotes proliferation
T106 518-534 UBERON_EXT:cell_clump_or_cluster_or_group_or_mass_or_population denotes cluster of cells
T107 529-534 CL_GO_EXT:cell denotes cells
T108 559-588 PR_EXT:000000183 denotes transforming growth factor β2
T109 572-578 GO_EXT:biological_growth_entity_or_process denotes growth
T110 572-585 GO_EXT:0008083 denotes growth factor
T111 589-598 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T112 638-651 GO_EXT:transcription denotes transcription
T113 638-658 GO_EXT:transcription_factor denotes transcription factor
T114 659-664 PR_EXT:000015308 denotes Snail
T115 686-693 CHEBI_EXT:52290 denotes mitogen
T116 686-718 GO_PR_EXT:MAP_kinase denotes mitogen-activated protein kinase
T117 704-711 CHEBI_PR_EXT:protein denotes protein
T118 720-724 GO_PR_EXT:MAP_kinase denotes MAPK
T119 753-762 UBERON:0007376 denotes epidermis
T120 764-769 PR_EXT:000015308 denotes Snail
T121 773-783 GO:0010467 denotes expression
T122 798-811 GO:0008283 denotes proliferation
T123 836-844 CL_GO_EXT:cell denotes cellular
T124 845-853 GO_PATO_EXT:biological_adhesion_entity_or_process denotes adhesion
T125 860-870 PR_EXT:000001447 denotes E-cadherin
T126 874-891 GO_EXT:transcription denotes transcriptionally
T127 892-906 GO_EXT:negative_regulation denotes down-regulated
T128 919-927 GO_PATO_EXT:biological_adhesion_entity_or_process denotes adhesion
T129 928-936 CHEBI_PR_EXT:protein denotes proteins
T130 960-969 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T131 974-982 GO_EXT:biological_release denotes released
T132 988-1006 GO:0005911 denotes cell-cell contacts
T133 988-992 _FRAGMENT denotes cell
T134 1121-1126 CL_GO_EXT:cell denotes cells
T135 993-997 CL_GO_EXT:cell denotes cell
T136 1052-1056 GO_PR_EXT:MAP_kinase denotes MAPK
T137 1052-1067 GO:0000187 denotes MAPK activation
T138 1168-1183 GO_EXT:transcription denotes transcriptional
T139 1207-1220 GO:0008283 denotes proliferation
T140 1275-1292 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling pathway
T141 1338-1348 GO_EXT:0016015 denotes morphogens
T142 1364-1379 GO_EXT:transcription denotes transcriptional
T143 1392-1397 GO_EXT:biological_direction_or_guidance denotes guide
T144 1398-1402 UBERON:0001037 denotes hair
T145 1439-1443 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T744 1640-1649 NCBITaxon:40674 denotes Mammalian
T745 1675-1688 GO:0009653 denotes morphogenesis
T746 1762-1777 UBERON_EXT:cell_clump_or_cluster_or_group_or_mass_or_population denotes masses of cells
T747 1772-1777 CL_GO_EXT:cell denotes cells
T748 1821-1833 _FRAGMENT denotes formation of
T749 1839-1845 GO:0048513 denotes organs
T750 1839-1845 UBERON:0000062 denotes organs
T751 1857-1862 UBERON:0002048 denotes lungs
T752 1864-1875 UBERON:0002240 denotes spinal cord
T753 1877-1891 UBERON:0001911 denotes mammary glands
T754 1897-1911 UBERON:0002073 denotes hair follicles
T755 1934-1942 GO_PATO_EXT:biological_adhesion_or_attachedness denotes adhering
T756 1943-1953 UBERON:0000483 denotes epithelial
T757 1943-1959 CL:0000066 denotes epithelial cells
T758 1954-1959 CL_GO_EXT:cell denotes cells
T759 2000-2012 GO_UBERON_EXT:basal_lamina denotes basal lamina
T760 2032-2048 UBERON:0010136 denotes epithelial sheet
T761 2083-2093 UBERON:0003104 denotes mesenchyme
T762 2103-2116 GO:0009653 denotes morphogenesis
T763 2120-2126 GO_EXT:biological_direction_or_guidance denotes guided
T764 2171-2181 UBERON:0000483 denotes epithelium
T765 2186-2196 UBERON:0003104 denotes mesenchyme
T766 2228-2233 UBERON:0000062 denotes organ
T767 2256-2262 GO:0065007 denotes govern
T768 2267-2273 GO_EXT:biological_growth_entity_or_process denotes growth
T769 2296-2305 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T770 2306-2328 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes communication pathways
T771 2339-2343 UBERON_EXT:bone_element_or_tissue denotes bone
T772 2339-2364 PR_EXT:000000034 denotes bone morphogenic proteins
T773 2344-2355 GO_EXT:0016015 denotes morphogenic
T774 2356-2364 CHEBI_PR_EXT:protein denotes proteins
T775 2366-2370 PR_EXT:000000034 denotes BMPs
T776 2373-2402 PR_EXT:000000046 denotes transforming growth factor βs
T777 2386-2392 GO_EXT:biological_growth_entity_or_process denotes growth
T778 2386-2399 GO_EXT:0008083 denotes growth factor
T779 2404-2410 PR_EXT:000000046 denotes TGF-βs
T780 2417-2427 CL:0000057 denotes fibroblast
T781 2428-2434 GO_EXT:biological_growth_entity_or_process denotes growth
T782 2428-2442 GO_EXT:0008083 denotes growth factors
T783 2473-2477 CL_GO_EXT:cell denotes cell
T784 2473-2485 GO:0009986 denotes cell surface
T785 2491-2499 GO:0016020 denotes membrane
T786 2500-2509 GO_EXT:0004872 denotes receptors
T787 2517-2525 GO:0005576 denotes external
T788 2526-2535 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T789 2526-2545 CHEBI_EXT:62488 denotes signaling molecules
T790 2563-2595 GO:0035556 denotes cascades of intracellular events
T791 2575-2588 GO:0005622 denotes intracellular
T792 2580-2588 CL_GO_EXT:cell denotes cellular
T793 2625-2629 SO_EXT:0000704 denotes gene
T794 2625-2640 GO:0010467 denotes gene expression
T795 2642-2648 GO_EXT:biological_growth_entity_or_process denotes growth
T796 2654-2669 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T797 2787-2800 GO:0009653 denotes morphogenetic
T798 2883-2892 GO_PATO_EXT:biological_patterning_process_or_quality denotes patterned
T799 3002-3011 CHEBI_EXT:35224 denotes effectors
T800 3048-3052 CL_GO_EXT:cell denotes cell
T801 3147-3155 GO_PATO_EXT:biological_behavior denotes behavior
T802 3172-3177 CL_GO_EXT:cell denotes cells
T803 3188-3204 UBERON:0010136 denotes epithelial sheet
T805 3309-3311 _FRAGMENT denotes of
T806 3321-3326 GO:0008283 denotes cells
T807 3321-3326 CL_GO_EXT:cell denotes cells
T808 3376-3388 GO_UBERON_EXT:basal_lamina denotes basal lamina
T809 3396-3409 GO:0005576 denotes extracellular
T810 3401-3409 CL_GO_EXT:cell denotes cellular
T811 3410-3420 UBERON:0000483 denotes epithelial
T812 3421-3432 UBERON:0003104 denotes mesenchymal
T813 3490-3506 _FRAGMENT denotes determination of
T814 3516-3526 GO:0001709 denotes cell fates
T815 3516-3520 CL_GO_EXT:cell denotes cell
T816 3612-3616 CL_GO_EXT:cell denotes cell
T817 3644-3650 UBERON:0000062 denotes organs
T818 3652-3666 UBERON:0002073 denotes hair follicles
T819 3708-3722 UBERON:0005153 denotes epithelial bud
T820 3734-3743 NCBITaxon:40674 denotes Mammalian
T821 3744-3759 UBERON:0019204 denotes skin epithelium
T822 3800-3810 UBERON:0000924 denotes ectodermal
T823 3800-3816 CL:0000221 denotes ectodermal cells
T824 3811-3816 CL_GO_EXT:cell denotes cells
T825 3850-3861 UBERON:0003104 denotes mesenchymal
T826 3850-3867 CL:0000134 denotes mesenchymal cells
T827 3862-3867 CL_GO_EXT:cell denotes cells
T828 3881-3885 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T829 3941-3951 UBERON:0000483 denotes epithelial
T830 3952-3963 UBERON:0003104 denotes mesenchymal
T831 4029-4039 _FRAGMENT denotes cluster of
T832 4051-4056 UBERON_EXT:cell_clump_or_cluster_or_group_or_mass_or_population denotes cells
T833 4040-4050 UBERON:0000483 denotes epithelial
T834 4040-4056 CL:0000066 denotes epithelial cells
T835 4051-4056 CL_GO_EXT:cell denotes cells
T836 4062-4069 UBERON_EXT:placode denotes placode
T837 4083-4091 _FRAGMENT denotes group of
T838 4115-4120 UBERON_EXT:cell_clump_or_cluster_or_group_or_mass_or_population denotes cells
T839 4103-4114 UBERON:0003104 denotes mesenchymal
T840 4103-4120 CL:0000134 denotes mesenchymal cells
T841 4115-4120 CL_GO_EXT:cell denotes cells
T842 4154-4168 UBERON:0000412 denotes dermal papilla
T843 4211-4224 UBERON:0002073 denotes hair follicle
T844 4259-4266 UBERON_EXT:placode denotes placode
T845 4279-4293 UBERON:0000412 denotes dermal papilla
T846 4312-4318 GO_EXT:biological_growth_entity_or_process denotes growth
T847 4326-4334 UBERON_EXT:follicle denotes follicle
T848 4355-4361 UBERON:0002067 denotes dermis
T849 4371-4377 GO_EXT:biological_growth_entity_or_process denotes growth
T850 4392-4407 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T851 4453-4461 UBERON_EXT:follicle denotes follicle
T852 4508-4518 UBERON:0000483 denotes epithelial
T853 4523-4534 UBERON:0003104 denotes mesenchymal
T854 4557-4560 PR_EXT:000000034 denotes BMP
T855 4579-4585 PR_EXT:000000021 denotes noggin
T856 4617-4625 CL_UBERON_EXT:lymphocyte_or_lymphoid_progenitor_or_system_or_tissue denotes lymphoid
T857 4617-4643 PR_EXT:000009751 denotes lymphoid enhancer factor-1
T858 4626-4634 SO_EXT:0000165 denotes enhancer
T859 4644-4653 PR_EXT:000002198 denotes β-catenin
T860 4655-4660 PR_EXT:000009751 denotes LEF-1
T861 4661-4670 PR_EXT:000002198 denotes β-catenin
T862 4681-4685 SO_EXT:0000704 denotes gene
T863 4686-4699 GO_EXT:transcription denotes transcription
T864 4711-4727 UBERON:0011817 denotes follicle placode
T865 4804-4822 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling pathways
T866 4831-4846 GO_EXT:negative_regulation denotes down-regulation
T867 4854-4858 SO_EXT:0000704 denotes gene
T868 4859-4867 SO_EXT:sequence_coding_function denotes encoding
T869 4868-4878 PR_EXT:000001447 denotes E-cadherin
T870 4897-4907 UBERON:0000483 denotes epithelial
T871 4908-4916 PR_EXT:000001327 denotes cadherin
T872 4937-4945 GO:0016020 denotes membrane
T873 4954-4986 GO:0005913 denotes intercellular adherens junctions
T874 4959-4967 CL_GO_EXT:cell denotes cellular
T875 4988-4991 GO:0005912 denotes AJs
T876 5031-5041 PR_EXT:000001447 denotes E-cadherin
T877 5045-5059 SO_EXT:transgenic_entity denotes transgenically
T878 5072-5077 NCBITaxon:10088 denotes mouse
T879 5078-5082 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T880 5084-5097 UBERON:0002073 denotes hair follicle
T881 5084-5111 GO:0031069 denotes hair follicle morphogenesis
T882 5140-5150 PR_EXT:000001447 denotes E-cadherin
T883 5151-5166 GO_EXT:negative_regulation denotes down-regulation
T884 5190-5199 GO:0065007 denotes governing
T885 5204-5212 GO_PATO_EXT:biological_adhesion_entity_or_process denotes adhesion
T886 5244-5257 GO:0009653 denotes morphogenesis
T887 5268-5273 PR_EXT:000009751 denotes LEF-1
T888 5275-5285 PR_EXT:000001447 denotes E-cadherin
T889 5291-5296 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binds
T890 5300-5309 PR_EXT:000002198 denotes β-catenin
T891 5323-5327 CL_GO_EXT:cell denotes cell
T892 5328-5332 CL_GO_EXT:cell denotes cell
T893 5351-5361 PR_EXT:000001447 denotes E-cadherin
T894 5362-5371 PR_EXT:000002198 denotes β-catenin
T895 5372-5381 GO:0032991 denotes complexes
T896 5382-5389 GO_EXT:biological_recruitment denotes recruit
T897 5390-5399 PR_EXT:alpha_catenin denotes α-catenin
T898 5442-5462 GO:0030041 denotes actin polymerization
T899 5448-5462 MOP:0000629 denotes polymerization
T900 5503-5506 GO:0005912 denotes AJs
T901 5525-5537 GO:0005856 denotes cytoskeleton
T902 5548-5564 UBERON:0010136 denotes epithelial sheet
T903 5574-5583 PR_EXT:alpha_catenin denotes α-Catenin
T904 5589-5594 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binds
T905 5612-5617 PR_EXT:P20154 denotes Lin-1
T906 5619-5624 PR_EXT:000009116 denotes Isl-1
T907 5626-5631 PR_EXT:P09088 denotes Mec-3
T908 5638-5645 CHEBI_PR_EXT:protein denotes protein
T909 5646-5651 PR_EXT:000009231 denotes Ajuba
T910 5669-5674 PR_EXT:000018242 denotes zyxin
T911 5685-5693 CHEBI_PR_EXT:protein denotes proteins
T912 5737-5745 GO_PATO_EXT:biological_adhesion_entity_or_process denotes adhesion
T913 5775-5782 CHEBI_EXT:52290 denotes mitogen
T914 5775-5807 GO_PR_EXT:MAP_kinase denotes mitogen-activated protein kinase
T915 5793-5800 CHEBI_PR_EXT:protein denotes protein
T916 5809-5813 GO_PR_EXT:MAP_kinase denotes MAPK
T917 5852-5855 GO:0005912 denotes AJs
T918 5878-5886 GO_PATO_EXT:biological_adhesion_entity_or_process denotes adhesion
T919 5892-5904 GO:0005856 denotes cytoskeletal
T920 5930-5937 GO:0005634 denotes nuclear
T921 5942-5953 GO:0005737 denotes cytoplasmic
T922 5954-5963 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T923 6015-6025 PR_EXT:000001447 denotes E-cadherin
T924 6169-6178 GO:0065007 denotes governing
T925 6179-6189 PR_EXT:000001447 denotes E-cadherin
T926 6190-6198 SO_EXT:0000167 denotes promoter
T927 6257-6262 PR_EXT:000009751 denotes LEF-1
T928 6263-6272 PR_EXT:000002198 denotes β-catenin
T929 6273-6280 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T930 6273-6285 SO_EXT:0000409 denotes binding site
T931 6305-6309 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes bind
T932 6314-6319 PR_EXT:000015308 denotes Snail
T933 6320-6324 PR_EXT:000015309 denotes Slug
T934 6335-6339 CHEBI_EXT:zinc denotes zinc
T935 6347-6362 GO_EXT:transcription denotes transcriptional
T936 6373-6381 CHEBI_PR_EXT:protein denotes proteins
T937 6414-6419 PR_EXT:000015308 denotes Snail
T938 6424-6439 GO_EXT:negative_regulation denotes down-regulation
T939 6443-6453 PR_EXT:000001447 denotes E-cadherin
T940 6476-6486 UBERON:0000483 denotes epithelial
T941 6476-6513 GO:0001837 denotes epithelial to mesenchymal transitions
T942 6490-6501 UBERON:0003104 denotes mesenchymal
T943 6515-6519 GO:0001837 denotes EMTs
T944 6567-6575 GO_PATO_EXT:biological_adhesion_or_attachedness denotes adhering
T945 6576-6586 UBERON:0000483 denotes epithelial
T946 6576-6592 CL:0000066 denotes epithelial cells
T947 6587-6592 CL_GO_EXT:cell denotes cells
T948 6598-6604 _FRAGMENT denotes motile
T949 6617-6622 CL:0000219 denotes cells
T950 6605-6616 UBERON:0003104 denotes mesenchymal
T951 6605-6622 CL:0000134 denotes mesenchymal cells
T952 6617-6622 CL_GO_EXT:cell denotes cells
T953 6662-6665 GO:0001837 denotes EMT
T954 6674-6684 PR_EXT:000001447 denotes E-cadherin
T955 6706-6720 GO_EXT:negative_regulation denotes down-regulated
T956 6748-6756 GO_PATO_EXT:biological_adhesion_or_adhesiveness denotes adhesive
T957 6867-6875 PR_EXT:000001327 denotes cadherin
T958 6876-6886 GO:0010467 denotes expression
T959 6894-6909 GO_EXT:transcription denotes transcriptional
T960 6917-6922 PR_EXT:000015308 denotes Snail
T961 6961-6966 PR_EXT:000009751 denotes LEF-1
T962 6967-6976 PR_EXT:000002198 denotes β-catenin
T963 6980-7001 GO_EXT:negative_regulation denotes negatively modulating
T964 7002-7012 PR_EXT:000001447 denotes E-cadherin
T965 7013-7021 SO_EXT:0000167 denotes promoter
T966 7077-7082 PR_EXT:000015308 denotes Snail
T967 7086-7095 GO:0010467 denotes expressed
T968 7125-7129 UBERON:0001037 denotes hair
T969 7150-7160 PR_EXT:000001447 denotes E-cadherin
T970 7161-7176 GO_EXT:negative_regulation denotes down-regulation
T971 7195-7208 GO:0008283 denotes proliferation
T972 7233-7238 PR_EXT:000015308 denotes Snail
T973 7287-7295 UBERON_EXT:follicle denotes follicle
T974 7301-7307 GO_EXT:biological_growth_entity_or_process denotes growth
T975 7312-7322 GO_PATO_EXT:biological_maturation denotes maturation
T976 7458-7462 UBERON:0001037 denotes hair
T977 7488-7503 GO_EXT:negative_regulation denotes down-regulation
T978 7507-7517 PR_EXT:000001447 denotes E-cadherin
T979 7529-7542 GO:0008283 denotes proliferation
T980 7567-7582 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T981 7615-7620 PR_EXT:000015308 denotes Snail
T982 7628-7632 UBERON:0001037 denotes hair
T983 7657-7661 CHEBI_SO_EXT:mRNA denotes mRNA
T984 7688-7701 GO:0016055 denotes Wnt signaling
T985 7706-7709 PR_EXT:000000034 denotes BMP
T986 7722-7727 PR_EXT:000009751 denotes LEF-1
T987 7728-7737 PR_EXT:000002198 denotes β-catenin
T988 7775-7780 PR_EXT:000015308 denotes Snail
T989 7781-7785 SO_EXT:0000704 denotes gene
T990 7781-7796 GO:0010467 denotes gene expression
T991 7800-7809 UBERON:0000922 denotes embryonic
T992 7810-7814 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T993 7815-7828 CL:0000312 denotes keratinocytes
T994 7864-7874 SO_EXT:transgenic_entity denotes transgenic
T995 7876-7878 SO_EXT:transgenic_entity denotes Tg
T996 7885-7889 SO_EXT:0000704 denotes gene
T997 7922-7928 PR_EXT:000000183 denotes TGF-β2
T998 7933-8003 PR_EXT:000000364 denotes small phenotype– and mothers against decapentaplegic–related protein 2
T999 7994-8001 CHEBI_PR_EXT:protein denotes protein
T1000 8005-8010 PR_EXT:000000364 denotes SMAD2
T1001 8012-8021 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T1002 8047-8052 PR_EXT:000015308 denotes Snail
T1003 8053-8057 SO_EXT:0000704 denotes gene
T1004 8053-8068 GO:0010467 denotes gene expression
T1005 8072-8087 UBERON:0019204 denotes skin epithelium
T1006 8107-8113 PR_EXT:000000183 denotes TGF-β2
T1007 8114-8123 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T1008 8128-8133 PR_EXT:000015308 denotes Snail
T1009 8134-8138 SO_EXT:0000704 denotes gene
T1010 8134-8149 GO:0010467 denotes gene expression
T1011 8151-8164 UBERON:0005086 denotes hair placodes
T1012 8187-8195 UBERON_EXT:follicle denotes follicle
T1013 8201-8207 GO_EXT:biological_growth_entity_or_process denotes growth
T1014 8255-8260 PR_EXT:000015308 denotes Snail
T1015 8292-8296 CL_GO_EXT:cell denotes cell
T1016 8292-8315 GO:0001708 denotes cell fate specification
T1017 8369-8382 GO:0008283 denotes proliferation
T1018 8387-8396 GO_EXT:biological_movement_or_translocation_process denotes migration
T2526 8408-8413 PR_EXT:000015308 denotes Snail
T2527 8414-8418 CHEBI_SO_EXT:mRNA denotes mRNA
T2528 8423-8430 CHEBI_PR_EXT:protein denotes Protein
T2529 8435-8444 GO:0010467 denotes Expressed
T2530 8464-8468 UBERON:0001037 denotes Hair
T2531 8482-8490 UBERON_EXT:follicle denotes Follicle
T2532 8491-8504 GO:0009653 denotes Morphogenesis
T2533 8514-8519 PR_EXT:000015308 denotes Snail
T2534 8571-8575 GO:0001837 denotes EMTs
T2535 8639-8654 GO_EXT:negative_regulation denotes down-regulation
T2536 8692-8715 GO:0005911 denotes intercellular junctions
T2537 8697-8705 CL_GO_EXT:cell denotes cellular
T2538 8768-8773 PR_EXT:000015308 denotes Snail
T2539 8836-8846 UBERON:0000483 denotes epithelial
T2540 8878-8891 UBERON:0002073 denotes hair follicle
T2541 8958-8963 PR_EXT:000015308 denotes Snail
T2542 8985-8990 PR_EXT:000009751 denotes LEF-1
T2543 8991-9000 PR_EXT:000002198 denotes β-catenin
T2544 9004-9019 GO_EXT:negative_regulation denotes down-regulating
T2545 9020-9030 PR_EXT:000001447 denotes E-cadherin
T2546 9031-9041 GO:0010467 denotes expression
T2547 9045-9058 CL:0000312 denotes keratinocytes
T2548 9109-9114 PR_EXT:000009751 denotes LEF-1
T2549 9115-9124 PR_EXT:000002198 denotes β-catenin
T2550 9128-9141 UBERON:0002073 denotes hair follicle
T2551 9128-9155 GO:0031069 denotes hair follicle morphogenesis
T2552 9196-9201 PR_EXT:000015308 denotes Snail
T2553 9202-9206 PR_EXT:000015309 denotes Slug
T2554 9295-9300 PR_EXT:000015308 denotes Snail
T2555 9301-9305 CHEBI_SO_EXT:mRNA denotes mRNA
T2556 9306-9316 GO:0010467 denotes expression
T2557 9336-9340 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T2558 9341-9354 GO:0009790 denotes embryogenesis
T2559 9369-9383 UBERON:0002073 denotes hair follicles
T2560 9446-9451 PR_EXT:000015308 denotes Snail
T2561 9452-9456 CHEBI_SO_EXT:mRNA denotes mRNA
T2562 9460-9469 GO:0010467 denotes expressed
T2563 9488-9492 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T2564 9515-9528 GO:0097617 denotes hybridization
T2565 9542-9547 CHEBI_SO_EXT:molecular_probe denotes probe
T2566 9562-9567 PR_EXT:000015308 denotes Snail
T2567 9568-9590 SO_EXT:0000205 denotes 3′ untranslated region
T2568 9568-9570 _FRAGMENT denotes 3′
T2569 9592-9595 SO_EXT:0000205 denotes UTR
T2570 9573-9583 GO:0006412 denotes translated
T2571 9598-9607 UBERON:0000922 denotes Embryonic
T2572 9665-9673 UBERON_EXT:follicle denotes follicle
T2573 9674-9687 GO:0009653 denotes morphogenesis
T2574 9734-9739 PR_EXT:000015308 denotes Snail
T2575 9740-9750 GO:0010467 denotes expression
T2576 9819-9832 GO:0097617 denotes hybridization
T2577 9857-9867 UBERON:0000483 denotes epithelium
T2578 9879-9883 UBERON:0001037 denotes hair
T2579 9906-9915 UBERON_EXT:follicle denotes follicles
T2580 10017-10030 GO:0097617 denotes hybridization
T2581 10068-10073 PR_EXT:000015308 denotes Snail
T2582 10074-10078 CHEBI_SO_EXT:mRNA denotes mRNA
T2583 10079-10089 GO:0010467 denotes expression
T2584 10097-10105 UBERON_EXT:follicle denotes follicle
T2585 10139-10149 GO:0097617 denotes hybridized
T2586 10150-10154 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T2587 10175-10184 UBERON_EXT:follicle denotes follicles
T2588 10213-10226 GO:0009653 denotes morphogenesis
T2589 10251-10262 GO:0097617 denotes Hybridizing
T2590 10263-10267 UBERON:0001037 denotes hair
T2591 10319-10330 GO:0097617 denotes hybridizing
T2592 10331-10340 UBERON_EXT:follicle denotes follicles
T2593 12805-12810 PR_EXT:000015308 denotes Snail
T2594 12811-12815 CHEBI_SO_EXT:mRNA denotes mRNA
T2595 12816-12826 GO:0010467 denotes expression
T2596 12847-12854 CHEBI_PR_EXT:protein denotes protein
T2597 12878-12886 GO:0042571 denotes antibody
T2598 12899-12909 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminal
T2599 12910-12918 SO_EXT:biological_sequence denotes sequence
T2600 12932-12940 SO_EXT:sequence_upstreamness denotes upstream
T2601 12953-12962 SO_EXT:biological_conservation_process_or_quality denotes conserved
T2602 12963-12967 CHEBI_EXT:zinc denotes zinc
T2603 12975-12982 SO_EXT:0000417 denotes domains
T2604 13024-13032 GO:0042571 denotes antibody
T2605 13059-13067 CHEBI_PR_EXT:protein denotes proteins
T2606 13082-13095 CL:0000312 denotes keratinocytes
T2607 13147-13160 CL:0000312 denotes keratinocytes
T2608 13173-13183 GO:0010467 denotes expressing
T2609 13205-13211 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T2610 13212-13217 PR_EXT:000015308 denotes Snail
T2611 13218-13225 CHEBI_PR_EXT:protein denotes protein
T2612 13243-13251 GO:0042571 denotes antibody
T2613 13315-13320 PR_EXT:000015308 denotes Snail
T2614 13360-13369 UBERON:0000922 denotes embryonic
T2615 13370-13375 NCBITaxon:10088 denotes mouse
T2616 13376-13380 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T2617 13444-13457 UBERON:0002073 denotes hair follicle
T2618 13444-13471 GO:0031069 denotes hair follicle morphogenesis
T2619 13486-13493 PATO_UBERON_EXT:neonate_or_newborn denotes newborn
T2620 13515-13519 UBERON:0010164 denotes hair
T2621 13527-13532 NCBITaxon:10088 denotes mouse
T2622 13639-13644 PR_EXT:000015308 denotes Snail
T2623 13669-13678 UBERON:0007376 denotes epidermis
T2624 13702-13709 UBERON_EXT:placode denotes placode
T2625 13759-13772 _FRAGMENT denotes commitment of
T2626 13785-13790 _FRAGMENT denotes cells
T2627 13817-13819 _FRAGMENT denotes to
T2628 13827-13836 GO:0045165 denotes cell fate
T2629 13785-13790 CL_GO_EXT:cell denotes cells
T2630 13785-13793 _FRAGMENT denotes cells of
T2631 13808-13816 CL:0000221 denotes ectoderm
T2632 13798-13816 UBERON:0000924 denotes embryonic ectoderm
T2633 13822-13826 UBERON:0001037 denotes hair
T2634 13822-13831 CL:0002559 denotes hair cell
T2635 13827-13831 CL_GO_EXT:cell denotes cell
T2636 13878-13891 GO:0097617 denotes hybridization
T2637 13906-13911 PR_EXT:000015308 denotes Snail
T2638 13912-13920 GO:0042571 denotes antibody
T2639 13921-13928 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T2640 13934-13938 _FRAGMENT denotes hair
T2641 13952-13961 UBERON:0002073 denotes follicles
T2642 14037-14042 PR_EXT:000015308 denotes Snail
T2643 14043-14051 GO:0042571 denotes antibody
T2644 14091-14098 CHEBI_PR_EXT:protein denotes protein
T2645 14124-14129 PR_EXT:000015308 denotes Snail
T2646 14157-14166 GO:0010467 denotes expressed
T2647 14170-14183 CL:0000312 denotes keratinocytes
T2648 14191-14195 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T2649 14273-14278 PR_EXT:000015308 denotes Snail
T2650 14287-14300 GO:0097617 denotes hybridization
T2651 14326-14331 PR_EXT:000015308 denotes Snail
T2652 14332-14342 GO:0010467 denotes expression
T2653 14350-14354 UBERON:0001037 denotes hair
T2654 14413-14418 PR_EXT:000015308 denotes Snail
T2655 14419-14426 CHEBI_PR_EXT:protein denotes protein
T2656 14431-14440 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T2657 14448-14457 _FRAGMENT denotes nuclei of
T2658 14471-14476 GO:0005634 denotes cells
T2659 14462-14466 UBERON:0001037 denotes hair
T2660 14471-14476 CL_GO_EXT:cell denotes cells
T2661 14523-14528 PR_EXT:000015308 denotes Snail
T2662 14551-14566 GO_EXT:transcription denotes transcriptional
T2663 14605-14610 PR_EXT:000015308 denotes Snail
T2664 14611-14619 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeling
T2665 14674-14682 UBERON_EXT:follicle denotes follicle
T2666 14683-14696 GO:0009653 denotes morphogenesis
T2667 14698-14703 PR_EXT:000015308 denotes Snail
T2668 14733-14744 UBERON:0010512 denotes guard hairs
T2669 14774-14779 UBERON:0001037 denotes hairs
T2670 14841-14846 NCBITaxon:10088 denotes mouse
T2671 14847-14851 UBERON:0010166 denotes coat
T2672 14909-14919 GO:0042571 denotes antibodies
T2673 14932-14945 GO:0008283 denotes proliferating
T2674 14946-14953 GO:0005634 denotes nuclear
T2675 14954-14961 CHEBI_EXT:59132 denotes antigen
T2676 14962-14966 PR_EXT:000010425 denotes Ki67
T2677 14982-14987 PR_EXT:000015308 denotes Snail
T2678 14988-14998 GO:0010467 denotes expression
T2679 15048-15056 UBERON_EXT:follicle denotes follicle
T2680 15070-15083 GO:0008283 denotes proliferation
T2681 15093-15099 GO_EXT:biological_growth_entity_or_process denotes growth
T2682 15139-15149 GO:0042571 denotes antibodies
T2683 15170-15184 GO_PATO_EXT:phosphorylation_process_or_quality denotes phosphorylated
T2684 15194-15198 GO_PR_EXT:MAP_kinase denotes MAPK
T2685 15207-15213 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes marked
T2686 15230-15243 GO:0008283 denotes proliferating
T2687 15245-15249 PR_EXT:000010425 denotes Ki67
T2688 15260-15265 CL_GO_EXT:cell denotes cells
T2689 15286-15291 CL_GO_EXT:cell denotes cells
T2690 15313-15317 UBERON:0001037 denotes hair
T2691 15349-15354 PR_EXT:000015308 denotes Snail
T2692 15369-15373 PR_EXT:000010425 denotes Ki67
T2693 15402-15406 UBERON:0001037 denotes hair
T2694 15455-15460 PR_EXT:000009751 denotes LEF-1
T2695 15461-15470 PR_EXT:000002198 denotes β-catenin
T2696 15506-15518 UBERON:0005086 denotes hair placode
T2697 15545-15553 UBERON_EXT:placode denotes placodes
T2698 15555-15559 UBERON:0001037 denotes hair
T2699 15575-15593 _FRAGMENT denotes down-regulation in
T2700 15605-15615 GO:0010629 denotes expression
T2701 15594-15604 PR_EXT:000001447 denotes E-cadherin
T3783 15654-15664 GO:0010467 denotes Expression
T3784 15668-15673 PR_EXT:000015308 denotes Snail
T3785 15685-15694 UBERON:0007376 denotes Epidermal
T3786 15700-15713 GO:0008283 denotes proliferation
T3787 15718-15733 GO_RO_EXT:developmental_differentiation_process denotes Differentiation
T3788 15745-15747 SO_EXT:transgenic_entity denotes Tg
T3789 15748-15753 NCBITaxon:10088 denotes Mouse
T3790 15754-15758 UBERON_EXT:zone_of_skin_or_skin_of_body denotes Skin
T3791 15781-15786 PR_EXT:000015308 denotes Snail
T3792 15787-15797 GO:0010467 denotes expression
T3793 15814-15818 UBERON:0001037 denotes hair
T3794 15846-15859 GO:0008283 denotes proliferation
T3795 15915-15925 GO:0010467 denotes expressing
T3796 15926-15931 PR_EXT:000015308 denotes Snail
T3797 15945-15950 NCBITaxon:10088 denotes mouse
T3798 15951-15965 UBERON:0001003 denotes skin epidermis
T3799 15982-15984 SO_EXT:transgenic_entity denotes Tg
T3800 16001-16006 PR_EXT:000015308 denotes Snail
T3801 16023-16030 CHEBI_SO_EXT:epitope denotes epitope
T3802 16062-16067 PR_EXT:000015308 denotes Snail
T3803 16070-16085 GO_EXT:transcription denotes transcriptional
T3804 16107-16110 PR_EXT:000009454 denotes K14
T3805 16111-16116 PR_EXT:000015308 denotes Snail
T3806 16121-16123 SO_EXT:transgenic_entity denotes Tg
T3807 16124-16131 NCBITaxon:33208 denotes animals
T3808 16149-16158 GO:0010467 denotes expressed
T3809 16163-16172 SO_EXT:0000902 denotes transgene
T3810 16213-16217 NCBITaxon:10088 denotes Mice
T3811 16238-16247 SO_EXT:0000902 denotes transgene
T3812 16262-16266 CL_GO_EXT:cell denotes cell
T3813 16273-16277 GO:0016265 denotes died
T3814 16298-16303 GO:0007567 denotes birth
T3815 16330-16332 SO_EXT:transgenic_entity denotes Tg
T3816 16341-16345 NCBITaxon:10088 denotes mice
T3817 16355-16364 SO_EXT:0000902 denotes transgene
T3818 16418-16423 PR_EXT:000015308 denotes Snail
T3819 16424-16428 SO_EXT:0000704 denotes gene
T3820 16424-16439 GO:0010467 denotes gene expression
T3821 16505-16516 GO_EXT:killing denotes sacrificing
T3822 16566-16571 GO:0007567 denotes birth
T3823 16576-16581 PR_EXT:000015308 denotes Snail
T3824 16582-16584 SO_EXT:transgenic_entity denotes Tg
T3825 16585-16592 NCBITaxon:33208 denotes animals
T3826 16593-16597 GO:0040007 denotes grew
T3827 16652-16657 UBERON:0002415 denotes tails
T3828 16669-16673 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T3829 16725-16729 NCBITaxon:10088 denotes mice
T3830 16764-16773 UBERON:0007376 denotes epidermal
T3831 16850-16852 SO_EXT:transgenic_entity denotes Tg
T3832 16853-16858 PR_EXT:000015308 denotes Snail
T3833 16859-16866 CHEBI_PR_EXT:protein denotes protein
T3834 16905-16915 GO:0010467 denotes expressing
T3835 16916-16923 GO:0005634 denotes nuclear
T3836 16927-16933 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T3837 16934-16939 PR_EXT:000015308 denotes Snail
T3838 17005-17018 GO:0008283 denotes proliferation
T3839 17035-17045 GO:0042571 denotes antibodies
T3840 17058-17071 GO:0008283 denotes proliferating
T3841 17072-17079 GO:0005634 denotes nuclear
T3842 17080-17087 CHEBI_EXT:59132 denotes antigen
T3843 17088-17092 PR_EXT:000010425 denotes Ki67
T3844 17139-17144 CL_GO_EXT:cell denotes cells
T3845 17208-17213 CL_GO_EXT:cell denotes cells
T3846 17214-17224 GO:0010467 denotes expressing
T3847 17225-17234 PR_EXT:keratin_6 denotes keratin 6
T3848 17261-17278 UBERON:0010402 denotes suprabasal layers
T3849 17287-17300 GO_PATO_EXT:cell_proliferation_or_proliferativity denotes proliferative
T3850 17301-17305 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T3851 19310-19320 GO:0010467 denotes Expression
T3852 19328-19333 PR_EXT:000015308 denotes Snail
T3853 19334-19343 SO_EXT:0000902 denotes transgene
T3854 19367-19382 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T3855 19395-19408 GO_PATO_EXT:cell_proliferation_or_proliferativity denotes proliferative
T3856 19409-19418 UBERON:0007376 denotes epidermis
T3857 19486-19499 GO:0008283 denotes proliferating
T3858 19512-19517 PR_EXT:000015308 denotes Snail
T3859 19518-19528 GO:0010467 denotes expression
T3860 19557-19563 _FRAGMENT denotes layers
T3861 19569-19576 UBERON:0002026 denotes spinous
T3862 19664-19673 PR_EXT:000009481 denotes keratin 5
T3863 19675-19677 PR_EXT:000009481 denotes K5
T3864 19717-19728 UBERON:0002025 denotes basal layer
T3865 19769-19774 PR_EXT:000015308 denotes Snail
T3866 19775-19779 SO_EXT:sequence_nullness denotes null
T3867 19780-19784 NCBITaxon:10088 denotes mice
T3868 19801-19813 GO:0007369 denotes gastrulation
T3869 19862-19867 PR_EXT:000015308 denotes Snail
T3870 19880-19884 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T3871 19880-19896 GO:0043588 denotes skin development
T3872 19937-19947 GO:0010467 denotes expression
T3873 19951-19956 PR_EXT:000015308 denotes Snail
T3874 19957-19964 CHEBI_PR_EXT:protein denotes protein
T3875 19986-19988 PR_EXT:000009481 denotes K5
T3876 19990-19994 PR_EXT:000010425 denotes Ki67
T3877 20006-20018 UBERON:0010402 denotes suprabasally
T3878 21927-21940 GO:0008283 denotes proliferation
T3879 21945-21960 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T3880 22010-22020 UBERON:0000483 denotes epithelial
T3881 22055-22064 UBERON:0007376 denotes epidermal
T3882 22112-22117 PR_EXT:000015308 denotes Snail
T3883 22122-22131 GO:0010467 denotes expressed
T3884 22137-22150 GO_PATO_EXT:cell_proliferation_or_proliferativity denotes proliferative
T3885 22151-22158 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T3886 22210-22218 PR_EXT:cyclin_D denotes cyclin D
T3887 22304-22321 GO_UBERON_EXT:basement_membrane denotes basement membrane
T3888 22383-22393 GO:0042571 denotes antibodies
T3889 22420-22434 GO:0030056 denotes hemidesmosomes
T3890 22457-22465 GO_PATO_EXT:biological_adhesion_entity_or_process denotes adhesion
T3891 22469-22490 CL:0002187 denotes basal epidermal cells
T3892 22475-22484 UBERON:0007376 denotes epidermal
T3893 22485-22490 CL_GO_EXT:cell denotes cells
T3894 22509-22521 GO_UBERON_EXT:basal_lamina denotes basal lamina
T3895 22599-22616 GO_UBERON_EXT:basement_membrane denotes basement membrane
T3896 22624-22628 UBERON:0001037 denotes hair
T3897 22636-22645 UBERON:0000922 denotes embryonic
T3898 22650-22657 PATO_UBERON_EXT:neonate_or_newborn denotes newborn
T3899 22658-22662 NCBITaxon:10088 denotes mice
T3900 22668-22673 PR_EXT:000015308 denotes Snail
T3901 22686-22695 GO:0010467 denotes expressed
T3902 22715-22732 GO_UBERON_EXT:basement_membrane denotes basement membrane
T3903 22748-22757 UBERON:0007376 denotes epidermis
T3904 22767-22773 UBERON:0002067 denotes dermis
T3905 22797-22802 UBERON:0007023 denotes adult
T3906 22803-22805 SO_EXT:transgenic_entity denotes Tg
T3907 22806-22813 NCBITaxon:33208 denotes animals
T3908 22895-22904 UBERON:0007376 denotes epidermis
T3909 22924-22932 UBERON_EXT:follicle denotes follicle
T4931 22958-22967 UBERON:0007376 denotes Epidermal
T4932 22973-22986 GO:0008283 denotes proliferation
T4933 22991-23006 GO_EXT:negative_regulation denotes Down-regulation
T4934 23010-23012 GO:0005912 denotes AJ
T4935 23013-23021 CHEBI_PR_EXT:protein denotes Proteins
T4936 23025-23030 PR_EXT:000015308 denotes Snail
T4937 23031-23033 SO_EXT:transgenic_entity denotes Tg
T4938 23034-23038 NCBITaxon:10088 denotes Mice
T4939 23054-23064 PR_EXT:000001447 denotes E-cadherin
T4940 23065-23073 SO_EXT:0000167 denotes promoter
T4941 23097-23102 PR_EXT:000015308 denotes Snail
T4942 23152-23162 PR_EXT:000001447 denotes E-cadherin
T4943 23167-23181 GO_EXT:negative_regulation denotes down-regulated
T4944 23185-23190 PR_EXT:000015308 denotes Snail
T4945 23191-23201 GO:0010467 denotes expressing
T4946 23202-23206 UBERON:0001037 denotes hair
T4947 23239-23249 PR_EXT:000001447 denotes E-cadherin
T4948 23260-23262 GO:0005912 denotes AJ
T4949 23263-23271 CHEBI_PR_EXT:protein denotes proteins
T4950 23295-23308 GO_PATO_EXT:cell_proliferation_or_proliferativity denotes proliferative
T4951 23309-23318 UBERON:0007376 denotes epidermis
T4952 23325-23327 SO_EXT:transgenic_entity denotes Tg
T4953 23328-23333 PR_EXT:000015308 denotes Snail
T4954 23408-23418 PR_EXT:000001447 denotes E-cadherin
T4955 23423-23432 PR_EXT:alpha_catenin denotes α-catenin
T4956 23489-23497 GO:0042571 denotes antibody
T4957 23521-23523 GO:0005912 denotes AJ
T4958 23524-23532 CHEBI_PR_EXT:protein denotes proteins
T4959 23534-23543 PR_EXT:000002198 denotes β-catenin
T4960 23548-23553 PR_EXT:000009231 denotes Ajuba
T4961 23637-23649 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T4962 23653-23662 PR_EXT:000002198 denotes β-catenin
T4963 23667-23672 PR_EXT:000009231 denotes Ajuba
T4964 23696-23707 GO:0005737 denotes cytoplasmic
T4965 23723-23727 CL_GO_EXT:cell denotes cell
T4966 23723-23740 GO:0005911 denotes cell-cell borders
T4967 23728-23732 CL_GO_EXT:cell denotes cell
T4968 27772-27781 UBERON:0007376 denotes epidermis
T4969 27866-27869 UBERON:0001690 denotes ear
T4970 27870-27874 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T4971 27904-27909 PR_EXT:000015308 denotes Snail
T4972 27910-27917 CHEBI_PR_EXT:protein denotes protein
T4973 27923-27932 GO:0010467 denotes expressed
T4974 27972-27976 UBERON:0001137 denotes back
T4975 27977-27981 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T4976 27986-27989 UBERON:0001690 denotes ear
T4977 27990-27994 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T4978 28014-28024 PR_EXT:000001447 denotes E-cadherin
T4979 28029-28038 PR_EXT:alpha_catenin denotes α-catenin
T4980 28076-28085 PR_EXT:000002198 denotes β-catenin
T4981 28090-28095 PR_EXT:000009231 denotes Ajuba
T4982 28287-28296 PR_EXT:alpha_catenin denotes α-catenin
T4983 28334-28349 GO_EXT:transcription denotes transcriptional
T4984 28364-28369 PR_EXT:000015308 denotes Snail
T4985 28390-28392 GO:0005912 denotes AJ
T4986 28390-28402 GO:0034333 denotes AJ formation
T4987 28429-28439 PR_EXT:000001447 denotes E-cadherin
T4988 28440-28444 SO_EXT:0000704 denotes gene
T4989 28440-28455 GO:0010467 denotes gene expression
T4990 28519-28528 PR_EXT:alpha_catenin denotes α-catenin
T4991 28567-28577 GO:0010467 denotes expression
T4992 28581-28591 PR_EXT:000001447 denotes E-cadherin
T4993 28595-28600 PR_EXT:000015308 denotes Snail
T4994 28601-28611 GO:0010467 denotes expressing
T4995 28612-28625 CL:0000312 denotes keratinocytes
T4996 28662-28673 GO:0009294 denotes transfected
T4997 28674-28687 CL:0000312 denotes keratinocytes
T4998 28688-28698 GO:0010467 denotes expressing
T4999 28702-28708 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T5000 28709-28714 PR_EXT:000015308 denotes Snail
T5001 28735-28745 PR_EXT:000001447 denotes E-cadherin
T5002 28750-28759 PR_EXT:alpha_catenin denotes α-catenin
T5003 28763-28767 CL_GO_EXT:cell denotes cell
T5004 28763-28780 GO:0005911 denotes cell-cell borders
T5005 28768-28772 CL_GO_EXT:cell denotes cell
T5006 28784-28794 GO:0010467 denotes expression
T5007 28811-28817 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes tagged
T5008 28818-28828 PR_EXT:000001447 denotes E-cadherin
T5009 28846-28850 CL_GO_EXT:cell denotes cell
T5010 28846-28862 GO:0005911 denotes cell-cell border
T5011 28851-28855 CL_GO_EXT:cell denotes cell
T5012 28863-28875 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T5013 28879-28889 PR_EXT:000001447 denotes E-cadherin
T5014 28890-28897 CHEBI_PR_EXT:protein denotes protein
T5015 28908-28915 SO_EXT:sequence_rescue_process denotes rescued
T5016 28920-28924 CL_GO_EXT:cell denotes cell
T5017 28920-28936 GO:0005911 denotes cell-cell border
T5018 28925-28929 CL_GO_EXT:cell denotes cell
T5019 28949-28958 PR_EXT:alpha_catenin denotes α-catenin
T5020 28995-29004 PR_EXT:alpha_catenin denotes α-catenin
T5021 29005-29015 GO:0010467 denotes expression
T5022 29020-29032 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T5023 29087-29092 PR_EXT:000015308 denotes Snail
T5024 29093-29110 GO_EXT:transcription denotes transcriptionally
T5025 29121-29130 PR_EXT:alpha_catenin denotes α-catenin
T5026 29196-29206 PR_EXT:000001447 denotes E-cadherin
T5027 29227-29238 GO:0006412 denotes translation
T5028 29242-29251 PR_EXT:alpha_catenin denotes α-catenin
T5029 29252-29256 CHEBI_SO_EXT:mRNA denotes mRNA
T5030 29289-29291 GO:0005912 denotes AJ
T5031 29292-29299 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T5032 29301-29303 SO_EXT:transgenic_entity denotes Tg
T5033 29304-29308 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T5034 29332-29341 GO:0016020 denotes membranes
T5035 29346-29369 GO:0005911 denotes intercellular junctions
T5036 29351-29359 CL_GO_EXT:cell denotes cellular
T5037 29475-29490 UBERON:0019204 denotes skin epithelium
T5038 29513-29517 UBERON:0001037 denotes hair
T5039 29533-29548 GO_EXT:negative_regulation denotes down-regulation
T5040 29561-29569 CHEBI_PR_EXT:protein denotes proteins
T5041 29588-29592 CL_GO_EXT:cell denotes cell
T5042 29593-29597 CL_GO_EXT:cell denotes cell
T5043 29633-29641 CL_GO_EXT:cell denotes cellular
T5044 29642-29650 GO_PATO_EXT:biological_adhesion_entity_or_process denotes adhesion
T5045 29677-29682 PR_EXT:000015308 denotes Snail
T5046 29683-29685 SO_EXT:transgenic_entity denotes Tg
T5047 29686-29695 UBERON:0007376 denotes epidermis
T5048 29700-29704 UBERON:0001037 denotes hair
T5049 29731-29744 GO_PATO_EXT:cell_proliferation_or_proliferativity denotes proliferative
T5050 29785-29800 GO_EXT:negative_regulation denotes down-regulation
T5051 29804-29806 GO:0005912 denotes AJ
T5052 29807-29815 CHEBI_PR_EXT:protein denotes proteins
T5053 29892-29897 PR_EXT:000015308 denotes Snail
T5054 29898-29900 SO_EXT:transgenic_entity denotes Tg
T5055 29901-29910 UBERON:0007376 denotes epidermis
T5056 29990-30000 PR_EXT:000001447 denotes E-cadherin
T5057 30020-30025 PR_EXT:000015308 denotes Snail
T5058 30047-30060 GO:0008283 denotes proliferation
T5059 30104-30115 GO:0009294 denotes transfected
T5060 30116-30129 CL:0000312 denotes keratinocytes
T5061 30130-30140 GO:0010467 denotes expressing
T5062 30141-30146 PR_EXT:000015308 denotes Snail
T5063 30216-30221 CL_GO_EXT:cell denotes cells
T5064 30237-30247 GO:0010467 denotes expression
T5065 30251-30261 PR_EXT:000001447 denotes E-cadherin
T5066 30267-30272 PR_EXT:000015308 denotes Snail
T5067 30363-30365 GO:0005912 denotes AJ
T5068 30377-30384 CHEBI_PR_EXT:protein denotes protein
T5069 30402-30413 GO:0051235 denotes sequestered
T5070 30421-30436 GO:0005886 denotes plasma membrane
T5071 30458-30471 GO:0008283 denotes proliferation
T5072 30472-30489 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling pathway
T5073 30495-30498 GO:0005912 denotes AJs
T5074 30553-30568 GO_EXT:negative_regulation denotes down-regulation
T5075 30572-30582 PR_EXT:000001447 denotes E-cadherin
T5076 30590-30603 _FRAGMENT denotes translocation
T5077 30617-30619 _FRAGMENT denotes to
T5078 30624-30631 GO:0051170 denotes nucleus
T5079 30607-30616 PR_EXT:000002198 denotes β-catenin
T5080 30624-30631 GO:0005634 denotes nucleus
T5081 30657-30662 SO_EXT:0000704 denotes genes
T5082 30672-30681 GO:0065007 denotes regulated
T5083 30689-30694 PR_EXT:000009751 denotes LEF-1
T5084 30695-30701 CL:0000084 denotes T cell
T5085 30697-30701 CL_GO_EXT:cell denotes cell
T5086 30725-30728 CHEBI_SO_EXT:DNA denotes DNA
T5087 30729-30736 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T5088 30737-30745 CHEBI_PR_EXT:protein denotes proteins
T5089 30774-30781 GO:0005634 denotes nuclear
T5090 30782-30790 PR_EXT:cyclin_D denotes cyclin D
T5091 30799-30812 GO_PATO_EXT:cell_proliferation_or_proliferativity denotes proliferative
T5092 30813-30818 PR_EXT:000015308 denotes Snail
T5093 30819-30821 SO_EXT:transgenic_entity denotes Tg
T5094 30822-30831 UBERON:0007376 denotes epidermis
T5095 30894-30902 PR_EXT:cyclin_D denotes cyclin D
T5096 30903-30907 SO_EXT:0000704 denotes gene
T5097 30934-30943 PR_EXT:000002198 denotes β-catenin
T5098 30944-30957 GO_EXT:transcription denotes transcription
T5099 30993-31000 GO:0005634 denotes nuclear
T5100 31001-31010 PR_EXT:000002198 denotes β-catenin
T5101 31018-31020 SO_EXT:transgenic_entity denotes Tg
T5102 31021-31030 UBERON:0007376 denotes epidermis
T5103 31036-31042 GO:0007618 denotes mating
T5104 31047-31052 PR_EXT:000015308 denotes Snail
T5105 31053-31055 SO_EXT:transgenic_entity denotes Tg
T5106 31056-31060 NCBITaxon:10088 denotes mice
T5107 31089-31094 NCBITaxon:10088 denotes mouse
T5108 31125-31130 PR_EXT:000009751 denotes LEF-1
T5109 31135-31144 PR_EXT:000002198 denotes β-catenin
T5110 31208-31219 GO:0005737 denotes cytoplasmic
T5111 31220-31225 PR_EXT:000009231 denotes Ajuba
T5112 31265-31278 GO_PATO_EXT:cell_proliferation_or_proliferativity denotes proliferative
T5113 31295-31300 PR_EXT:000015308 denotes Snail
T5114 31301-31303 SO_EXT:transgenic_entity denotes Tg
T5115 31304-31313 UBERON:0007376 denotes epidermis
T5116 31356-31360 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes bind
T5117 31361-31370 PR_EXT:alpha_catenin denotes α-catenin
T5118 31377-31382 PR_EXT:000009231 denotes Ajuba
T5119 31407-31413 GO_EXT:biological_growth_entity_or_process denotes growth
T5120 31407-31420 GO_EXT:0008083 denotes growth factor
T5121 31407-31445 PR_EXT:000008224 denotes growth factor receptor-bound protein-2
T5122 31421-31429 GO_EXT:0004872 denotes receptor
T5123 31430-31435 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes bound
T5124 31436-31443 CHEBI_PR_EXT:protein denotes protein
T5125 31447-31452 PR_EXT:000008224 denotes Grb-2
T5126 31454-31470 PR_EXT:P26675 denotes son of sevenless
T5127 31472-31475 PR_EXT:P26675 denotes Sos
T5128 31482-31492 CHEBI_SO_EXT:nucleotide denotes nucleotide
T5129 31482-31508 GO_EXT:nucleotide_exchange_factor denotes nucleotide exchange factor
T5130 31541-31559 GO:0000187 denotes activation of MAPK
T5131 31555-31559 GO_PR_EXT:MAP_kinase denotes MAPK
T5132 31605-31607 SO_EXT:transgenic_entity denotes Tg
T5133 31608-31612 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T5134 31647-31652 PR_EXT:000009231 denotes Ajuba
T5135 31676-31683 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T5136 31695-31700 PR_EXT:000015308 denotes Snail
T5137 31701-31711 GO:0010467 denotes expressing
T5138 31712-31721 UBERON:0007376 denotes epidermis
T5139 31738-31743 PR_EXT:000009231 denotes Ajuba
T5140 31773-31778 PR_EXT:000008224 denotes Grb-2
T5141 31801-31808 CHEBI_PR_EXT:protein denotes protein
T5142 31822-31827 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skins
T5143 31831-31836 PR_EXT:000015308 denotes Snail
T5144 31837-31839 SO_EXT:transgenic_entity denotes Tg
T5145 31840-31844 NCBITaxon:10088 denotes mice
T5146 31876-31885 SO_EXT:wild_type_entity_or_quality denotes wild-type
T5147 31887-31889 SO_EXT:wild_type_entity_or_quality denotes WT
T5148 31891-31898 NCBITaxon:33208 denotes animals
T5149 31954-31963 PR_EXT:alpha_catenin denotes α-catenin
T5150 31964-31968 SO_EXT:sequence_nullness denotes null
T5151 31969-31978 UBERON:0007376 denotes epidermis
T5152 31990-31995 PR_EXT:000008224 denotes Grb-2
T5153 31996-32001 PR_EXT:000009231 denotes Ajuba
T5154 32080-32087 CHEBI_PR_EXT:protein denotes protein
T5155 32121-32125 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T5156 32194-32203 PR_EXT:alpha_catenin denotes α-catenin
T5157 32222-32227 PR_EXT:000015308 denotes Snail
T5158 32237-32252 GO_EXT:negative_regulation denotes down-regulation
T5159 32256-32266 PR_EXT:000001447 denotes E-cadherin
T5160 32273-32282 PR_EXT:alpha_catenin denotes α-catenin
T5161 32313-32318 PR_EXT:000009231 denotes Ajuba
T5162 32336-32341 PR_EXT:000008224 denotes Grb-2
T5163 32342-32345 PR_EXT:P26675 denotes Sos
T5164 32374-32379 PR_EXT:000008224 denotes Grb-2
T5165 32380-32383 PR_EXT:P26675 denotes Sos
T5166 32388-32397 PR_EXT:alpha_catenin denotes α-catenin
T5167 32402-32407 PR_EXT:000009231 denotes Ajuba
T5168 32445-32458 GO_PATO_EXT:cell_proliferation_or_proliferativity denotes proliferative
T5169 32470-32482 CL:0000312 denotes keratinocyte
T5170 32493-32503 GO:0010467 denotes expression
T5171 32507-32512 PR_EXT:000009231 denotes Ajuba
T5172 32591-32595 GO_PR_EXT:MAP_kinase denotes MAPK
T5173 32607-32609 SO_EXT:wild_type_entity_or_quality denotes WT
T5174 32610-32623 CL:0000312 denotes keratinocytes
T5175 32638-32643 UBERON:0001977 denotes serum
T5176 32652-32665 CL:0000312 denotes keratinocytes
T5177 32683-32694 GO:0009294 denotes transfected
T5178 32703-32708 PR_EXT:000009231 denotes Ajuba
T5179 32709-32719 GO:0010467 denotes expression
T5180 32720-32726 SO_EXT:0000440 denotes vector
T5181 32800-32811 GO:0009294 denotes transfected
T5182 32821-32824 PR_EXT:000009454 denotes K14
T5183 32833-32842 SO_EXT:0000902 denotes transgene
T5184 32891-32896 CL_GO_EXT:cell denotes cells
T5185 32923-32930 CHEBI_SO_EXT:peptide_or_peptide_region denotes peptide
T5186 32931-32940 CHEBI_EXT:35222 denotes inhibitor
T5187 32974-32979 PR_EXT:000008224 denotes Grb-2
T5188 32980-32983 PR_EXT:P26675 denotes Sos
T5189 33038-33043 PR_EXT:000009231 denotes Ajuba
T5190 33054-33060 SO_EXT:0000417 denotes domain
T5191 33097-33102 PR_EXT:000008224 denotes Grb-2
T5192 33105-33108 PR_EXT:000027234 denotes Src
T5193 33109-33117 SO:0000857 denotes homology
T5194 33120-33126 SO_EXT:0000417 denotes domain
T5195 33142-33148 SO_EXT:0000417 denotes domain
T5196 33157-33166 GO:0010467 denotes expressed
T5197 33170-33175 UBERON:0001977 denotes serum
T5198 33184-33197 CL:0000312 denotes keratinocytes
T5199 33280-33287 CHEBI_SO_EXT:peptide_or_peptide_region denotes peptide
T5200 33288-33297 CHEBI_EXT:35222 denotes inhibitor
T5201 33318-33323 PR_EXT:000008224 denotes Grb-2
T5202 33324-33327 PR_EXT:P26675 denotes Sos
T5203 33400-33409 GO:0005829 denotes cytosolic
T5204 33410-33415 PR_EXT:000009231 denotes Ajuba
T5205 33424-33429 PR_EXT:000009231 denotes Ajuba
T5206 33440-33446 SO_EXT:0000417 denotes domain
T5207 33466-33471 PR_EXT:000008224 denotes Grb-2
T5208 33472-33475 PR_EXT:P26675 denotes Sos
T5209 33508-33518 CHEBI_SO_EXT:nucleotide denotes nucleotide
T5210 33572-33576 GO_PR_EXT:MAP_kinase denotes MAPK
T5211 33640-33645 PR_EXT:000015308 denotes Snail
T5212 33646-33656 GO:0010467 denotes expression
T5213 33661-33674 GO:0008283 denotes proliferation
T5214 33693-33708 UBERON:0019204 denotes skin epithelium
T5215 33710-33723 GO_PATO_EXT:cell_proliferation_or_proliferativity denotes proliferative
T5216 33746-33749 GO:0005912 denotes AJs
T5217 33884-33893 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T7031 33914-33927 _FRAGMENT denotes Regulation of
T7032 33934-33949 GO:0010468 denotes Gene Expression
T7033 33928-33933 PR_EXT:000015308 denotes Snail
T7034 33934-33938 SO_EXT:0000704 denotes Gene
T7035 33979-33985 PR_EXT:000000183 denotes TGF-β2
T7036 33986-33995 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes Signaling
T7037 34014-34018 UBERON:0001037 denotes Hair
T7038 34045-34050 PR_EXT:000015308 denotes Snail
T7039 34051-34055 CHEBI_SO_EXT:mRNA denotes mRNA
T7040 34056-34066 GO:0010467 denotes expression
T7041 34074-34078 UBERON:0001037 denotes hair
T7043 34144-34149 PR_EXT:000015308 denotes Snail
T7044 34150-34154 SO_EXT:0000704 denotes gene
T7045 34169-34182 GO:0005576 denotes extracellular
T7046 34174-34182 CL_GO_EXT:cell denotes cellular
T7047 34213-34217 CL_GO_EXT:cell denotes cell
T7048 34232-34242 GO:0010467 denotes expression
T7049 34256-34261 PR_EXT:000015308 denotes Snail
T7050 34312-34316 PR_EXT:000000034 denotes BMPs
T7051 34328-34334 PR_EXT:000000046 denotes TGF-βs
T7052 34348-34352 UBERON:0001037 denotes hair
T7053 34386-34391 PR_EXT:000015308 denotes Snail
T7054 34399-34408 GO:0010467 denotes expressed
T7055 34421-34425 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T7056 34426-34439 CL:0000312 denotes keratinocytes
T7057 34445-34452 GO:0046903 denotes secrete
T7058 34460-34464 PR_EXT:000000034 denotes BMPs
T7059 34519-34522 _FRAGMENT denotes Wnt
T7060 34533-34542 GO:0016055 denotes signaling
T7061 34527-34532 PR_EXT:000000046 denotes TGF-β
T7062 34527-34542 GO:0007179 denotes TGF-β signaling
T7063 34573-34578 PR_EXT:000015308 denotes Snail
T7064 34597-34601 CL_GO_EXT:cell denotes cell
T7065 34645-34660 _FRAGMENT denotes transmission of
T7066 34670-34676 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signal
T7067 34663-34669 PR_EXT:000017443 denotes Wnt-3a
T7068 34689-34702 CL:0000312 denotes keratinocytes
T7069 34749-34752 PR_EXT:000000034 denotes BMP
T7070 34753-34762 CHEBI_EXT:35222 denotes inhibitor
T7071 34763-34769 PR_EXT:000000021 denotes noggin
T7072 34785-34790 PR_EXT:000009751 denotes LEF-1
T7073 34791-34801 GO:0010467 denotes expression
T7074 34834-34848 GO_EXT:negative_regulation denotes down-regulated
T7075 34853-34863 PR_EXT:000001447 denotes E-cadherin
T7076 34864-34872 SO_EXT:0000167 denotes promoter
T7077 34887-34892 PR_EXT:000009751 denotes LEF-1
T7078 34893-34902 PR_EXT:000002198 denotes β-catenin
T7079 34922-34926 SO_EXT:0000704 denotes gene
T7080 34955-34960 PR_EXT:000015308 denotes Snail
T7081 34961-34971 GO:0010467 denotes expression
T7082 35064-35070 PR_EXT:000000021 denotes noggin
T7083 35074-35087 UBERON:0002073 denotes hair follicle
T7084 35180-35189 GO:0065007 denotes governing
T7085 35190-35195 PR_EXT:000015308 denotes Snail
T7086 35196-35206 GO:0010467 denotes expression
T7087 35210-35223 CL:0000312 denotes keratinocytes
T7088 37336-37342 PR_EXT:000000182 denotes TGF-β1
T7089 37368-37373 PR_EXT:000015308 denotes Snail
T7090 37392-37403 CL:0000182 denotes hepatocytes
T7091 37408-37413 UBERON:0000948 denotes heart
T7092 37427-37440 CL:0000312 denotes keratinocytes
T7093 37451-37457 PR_EXT:000000182 denotes TGF-β1
T7094 37467-37479 CL:0000312 denotes keratinocyte
T7095 37480-37486 GO_EXT:biological_growth_entity_or_process denotes growth
T7096 37563-37576 UBERON:0002073 denotes hair follicle
T7097 37607-37616 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T7098 37642-37647 PR_EXT:000000046 denotes TGF-β
T7099 37648-37653 SO_EXT:0000704 denotes genes
T7100 37664-37670 PR_EXT:000000183 denotes TGF-β2
T7101 37671-37675 SO_EXT:sequence_nullness denotes null
T7102 37690-37698 UBERON_EXT:follicle denotes follicle
T7103 37718-37722 UBERON:0001037 denotes hair
T7104 37782-37788 PR_EXT:000000183 denotes TGF-β2
T7105 37810-37820 GO:0065007 denotes regulating
T7106 37821-37826 PR_EXT:000015308 denotes Snail
T7107 37827-37837 GO:0010467 denotes expression
T7108 37841-37854 CL:0000312 denotes keratinocytes
T7109 37873-37887 _FRAGMENT denotes basal layer of
T7110 37892-37901 UBERON:0002025 denotes epidermis
T7111 37922-37926 CL_GO_EXT:cell denotes cell
T7112 37974-37982 UBERON_EXT:placode denotes placodal
T7113 37983-37988 CL_GO_EXT:cell denotes cells
T7114 37996-38009 CL:0000312 denotes keratinocytes
T7115 38089-38097 GO_PATO_EXT:biological_behavior denotes behavior
T7116 38101-38111 UBERON:0000483 denotes epithelial
T7117 38101-38117 CL:0000066 denotes epithelial cells
T7118 38112-38117 CL_GO_EXT:cell denotes cells
T7119 38125-38132 UBERON_EXT:placode denotes placode
T7120 38171-38184 CL:0000312 denotes keratinocytes
T7121 38214-38220 PR_EXT:000000183 denotes TGF-β2
T7122 38263-38268 PR_EXT:000015308 denotes Snail
T7123 38308-38313 PR_EXT:000015308 denotes Snail
T7124 38314-38321 CHEBI_PR_EXT:protein denotes protein
T7125 38412-38417 PR_EXT:000015308 denotes Snail
T7126 38469-38475 GO_EXT:biological_growth_entity_or_process denotes growth
T7127 38469-38482 GO_EXT:0008083 denotes growth factor
T7128 38503-38509 PR_EXT:000000183 denotes TGF-β2
T7129 38547-38555 GO_EXT:reaction_or_response denotes response
T7130 38609-38614 PR_EXT:000000046 denotes TGF-β
T7131 38609-38623 GO_EXT:0005024 denotes TGF-β receptor
T7132 38721-38736 GO_PATO_EXT:phosphorylation_process denotes phosphorylation
T7133 38755-38763 CHEBI_EXT:35224 denotes effector
T7134 38764-38769 PR_EXT:000000364 denotes SMAD2
T7135 38771-38776 PR_EXT:000015308 denotes Snail
T7136 38777-38787 GO:0010467 denotes expression
T7137 38840-38845 PR_EXT:000015308 denotes Snail
T7138 38846-38856 GO:0010467 denotes expression
T7139 38873-38887 GO_PATO_EXT:phosphorylation_process_or_quality denotes phosphorylated
T7140 38888-38893 PR_EXT:000000364 denotes SMAD2
T7141 38995-39000 PR_EXT:000015308 denotes Snail
T7142 39001-39011 GO:0010467 denotes expression
T7143 39034-39038 CHEBI_SO_EXT:mRNA denotes mRNA
T7144 39062-39067 PR_EXT:000015308 denotes Snail
T7145 39068-39076 SO_EXT:0000167 denotes promoter
T7146 39089-39102 CL:0000312 denotes keratinocytes
T7147 39125-39131 PR_EXT:000000183 denotes TGF-β2
T7148 39132-39141 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T7149 39197-39206 SO_EXT:0000902 denotes transgene
T7150 39219-39234 GO_EXT:0004565 denotes β-galactosidase
T7151 39254-39261 GO:0065007 denotes control
T7152 39284-39285 CHEBI_SO_EXT:base denotes b
T7153 39289-39297 SO_EXT:0000167 denotes promoter
T7154 39298-39306 SO_EXT:biological_sequence denotes sequence
T7155 39315-39317 SO_EXT:sequence_upstreamness denotes 5′
T7156 39327-39340 GO_EXT:transcription denotes transcription
T7157 39327-39356 SO_EXT:0000315 denotes transcription initiation site
T7158 39352-39359 _FRAGMENT denotes site of
T7159 39376-39380 SO_EXT:0000842 denotes gene
T7160 39364-39369 NCBITaxon:10088 denotes mouse
T7161 39370-39375 PR_EXT:000015308 denotes Snail
T7162 39405-39417 GO:0009294 denotes transfection
T7163 39419-39432 CL:0000312 denotes keratinocytes
T7164 39451-39457 PR_EXT:000000183 denotes TGF-β2
T7165 39487-39496 SO_EXT:0000902 denotes transgene
T7166 39557-39562 PR_EXT:000015308 denotes Snail
T7167 39563-39570 CHEBI_PR_EXT:protein denotes protein
T7168 39657-39663 PR_EXT:000000183 denotes TGF-β2
T7169 39675-39680 PR_EXT:000015308 denotes Snail
T7170 39681-39689 SO_EXT:0000167 denotes promoter
T7171 39813-39818 PR_EXT:000015308 denotes Snail
T7172 39819-39827 SO_EXT:0000167 denotes promoter
T7173 39895-39908 CL:0000312 denotes keratinocytes
T7174 39926-39931 PR_EXT:000015308 denotes Snail
T7175 39932-39940 SO_EXT:0000167 denotes promoter
T7176 39988-39993 PR_EXT:000015308 denotes Snail
T7177 39994-40001 CHEBI_PR_EXT:protein denotes protein
T7178 40074-40080 PR_EXT:000000183 denotes TGF-β2
T7179 40143-40149 PR_EXT:000000183 denotes TGF-β2
T7180 40150-40157 CHEBI_PR_EXT:protein denotes protein
T7181 40164-40172 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T7182 40187-40191 GO_PR_EXT:SMAD denotes SMAD
T7183 40192-40199 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T7184 40192-40207 SO_EXT:0000409 denotes binding element
T7185 40227-40228 CHEBI_SO_EXT:base denotes b
T7186 40229-40231 SO_EXT:sequence_upstreamness denotes 5′
T7187 40241-40246 PR_EXT:000015308 denotes Snail
T7188 40247-40260 GO_EXT:transcription denotes transcription
T7189 40247-40271 SO_EXT:0000315 denotes transcription start site
T7190 40333-40346 CL:0000312 denotes keratinocytes
T7191 40348-40354 PR_EXT:000000183 denotes TGF-β2
T7192 40355-40364 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T7193 40423-40428 PR_EXT:000015308 denotes Snail
T7194 40429-40433 SO_EXT:0000704 denotes gene
T7195 40459-40464 PR_EXT:000015308 denotes Snail
T7196 40465-40472 CHEBI_PR_EXT:protein denotes protein
T7197 40505-40513 SO_EXT:0000167 denotes promoter
T7198 40539-40544 PR_EXT:000015308 denotes Snail
T7199 40545-40549 SO_EXT:0000704 denotes gene
T7200 40554-40561 CHEBI_PR_EXT:protein denotes protein
T7201 40575-40581 PR_EXT:000000183 denotes TGF-β2
T7202 40599-40612 CL:0000312 denotes keratinocytes
T7203 40650-40655 PR_EXT:000015308 denotes Snail
T7204 40656-40660 CHEBI_SO_EXT:mRNA denotes mRNA
T7205 40665-40672 CHEBI_PR_EXT:protein denotes protein
T7206 40694-40707 UBERON:0002073 denotes hair follicle
T7207 40694-40721 GO:0031069 denotes hair follicle morphogenesis
T7208 40725-40734 UBERON:0000922 denotes embryonic
T7209 40735-40740 NCBITaxon:10088 denotes mouse
T7210 40741-40745 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T7211 40763-40769 PR_EXT:000000183 denotes TGF-β2
T7212 40792-40797 PR_EXT:000015308 denotes Snail
T7213 40811-40815 UBERON:0001037 denotes hair
T7214 40865-40871 PR_EXT:000000183 denotes TGF-β2
T7215 40876-40885 GO:0010467 denotes expressed
T7216 40903-40907 UBERON:0001037 denotes hair
T7217 40965-40971 PR_EXT:000000183 denotes TGF-β2
T7218 40972-40980 GO:0042571 denotes antibody
T7219 40981-40988 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T7220 41000-41004 UBERON:0001037 denotes hair
T7221 41028-41036 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeling
T7222 41098-41106 UBERON_EXT:follicle denotes follicle
T7223 41117-41121 NCBITaxon:10088 denotes mice
T7224 41139-41145 PR_EXT:000000183 denotes TGF-β2
T7225 41146-41150 SO_EXT:sequence_nullness denotes null
T7226 41151-41159 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T7227 41204-41212 CHEBI_EXT:35224 denotes effector
T7228 41216-41222 PR_EXT:000000183 denotes TGF-β2
T7229 41223-41232 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T7230 41251-41260 GO:0010467 denotes expressed
T7231 41264-41266 SO_EXT:wild_type_entity_or_quality denotes WT
T7232 41276-41282 PR_EXT:000000183 denotes TGF-β2
T7233 41283-41287 SO_EXT:sequence_nullness denotes null
T7234 41289-41293 UBERON:0001037 denotes hair
T7235 41366-41372 PR_EXT:000000183 denotes TGF-β2
T7236 41373-41380 SO_EXT:0001060 denotes isoform
T7237 41389-41399 GO:0010467 denotes expression
T7238 41408-41414 PR_EXT:000000182 denotes TGF-β1
T7239 41419-41425 PR_EXT:000000183 denotes TGF-β2
T7240 41440-41444 UBERON:0001037 denotes hair
T7241 42509-42514 PR_EXT:000015308 denotes Snail
T7242 42519-42525 PR_EXT:000000183 denotes TGF-β2
T7243 42553-42558 PR_EXT:000015308 denotes Snail
T7244 42559-42569 GO:0010467 denotes expression
T7245 42573-42579 PR_EXT:000000183 denotes TGF-β2
T7246 42580-42584 SO_EXT:sequence_nullness denotes null
T7247 42585-42589 UBERON:0001037 denotes hair
T7248 42631-42636 PR_EXT:000015308 denotes Snail
T7249 42637-42644 CHEBI_PR_EXT:protein denotes protein
T7250 42667-42671 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T7251 42675-42681 PR_EXT:000000183 denotes TGF-β2
T7252 42682-42686 SO_EXT:sequence_nullness denotes null
T7253 42687-42694 UBERON:0000922 denotes embryos
T7254 42791-42806 GO_EXT:transcription denotes transcriptional
T7255 42820-42825 PR_EXT:000015308 denotes Snail
T7256 42826-42831 CHEBI_SO_EXT:mRNA denotes mRNAs
T7257 42855-42861 PR_EXT:000000183 denotes TGF-β2
T7258 42862-42866 SO_EXT:sequence_nullness denotes null
T7259 42867-42871 UBERON:0001037 denotes hair
T7260 42942-42946 UBERON:0001037 denotes hair
T7261 42966-42970 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T7262 43008-43011 PR_EXT:000009454 denotes K14
T7263 43012-43017 PR_EXT:000000364 denotes Smad2
T7264 43018-43020 SO_EXT:transgenic_entity denotes Tg
T7265 43021-43025 NCBITaxon:10088 denotes mice
T7266 43050-43055 PR_EXT:000000046 denotes TGF-β
T7267 43050-43065 GO:0007179 denotes TGF-β signaling
T7268 43069-43073 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T7269 43080-43085 PR_EXT:000015308 denotes Snail
T7270 43086-43093 CHEBI_PR_EXT:protein denotes protein
T7271 43171-43176 GO:0007567 denotes natal
T7272 43177-43181 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T7273 43258-43264 PR_EXT:000000183 denotes TGF-β2
T7274 43304-43309 PR_EXT:000015308 denotes Snail
T7275 43310-43314 SO_EXT:0000704 denotes gene
T7276 43310-43325 GO:0010467 denotes gene expression
T7277 43368-43372 UBERON:0001037 denotes hair
T7278 43422-43427 PR_EXT:000015308 denotes Snail
T7279 43497-43501 UBERON:0001037 denotes hair
T7280 43523-43529 PR_EXT:000000183 denotes TGF-β2
T7281 43530-43534 SO_EXT:sequence_nullness denotes null
T7282 43535-43539 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T7283 43632-43638 PR_EXT:000000183 denotes TGF-β2
T7284 43639-43648 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T7285 43678-43688 UBERON:0000483 denotes epithelial
T7286 43746-43759 GO:0009653 denotes morphogenesis
T7287 43790-43807 GO_UBERON_EXT:basement_membrane denotes basement membrane
T7288 43843-43849 PR_EXT:000000183 denotes TGF-β2
T7289 43850-43854 SO_EXT:sequence_nullness denotes null
T7290 43898-43908 GO:0042571 denotes antibodies
T7291 43917-43928 PR_EXT:000009140 denotes β4 integrin
T7292 43920-43928 GO_PR_EXT:integrin denotes integrin
T7293 43955-43967 CL:0000312 denotes keratinocyte
T7294 43977-43985 GO_PATO_EXT:biological_adhesion_entity_or_process denotes adhesion
T7295 44004-44021 GO_UBERON_EXT:basement_membrane denotes basement membrane
T7296 44048-44054 PR_EXT:000000183 denotes TGF-β2
T7297 44055-44064 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T7298 44114-44127 GO:0008283 denotes proliferation
T7329 44892-44905 GO_EXT:transcription denotes transcription
T7330 44922-44926 UBERON:0001037 denotes hair
T7331 44982-44984 SO_EXT:wild_type_entity_or_quality denotes WT
T7332 44989-44995 PR_EXT:000000183 denotes TGF-β2
T7333 44996-45000 SO_EXT:sequence_nullness denotes null
T7335 45024-45029 PR_EXT:000009751 denotes LEF-1
T7336 45034-45043 PR_EXT:000002198 denotes β-catenin
T7337 45044-45056 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T7338 45073-45076 _FRAGMENT denotes Wnt
T7339 45084-45101 GO:0016055 denotes signaling pathway
T7340 45077-45083 PR_EXT:000000021 denotes noggin
T7341 45145-45158 UBERON:0002073 denotes hair follicle
T7342 45145-45172 GO:0031069 denotes hair follicle morphogenesis
T7343 45174-45180 PR_EXT:000000183 denotes TGF-β2
T7344 45211-45217 PR_EXT:000000021 denotes noggin
T7345 45244-45248 UBERON:0001037 denotes hair
T7346 45287-45292 PR_EXT:000015308 denotes Snail
T7347 45293-45297 SO_EXT:0000704 denotes gene
T7348 45293-45308 GO:0010467 denotes gene expression
T7349 45310-45316 PR_EXT:000000183 denotes TGF-β2
T7350 45360-45370 GO_EXT:0016015 denotes morphogens
T7351 45374-45387 GO_EXT:negative_regulation denotes down-regulate
T7352 45388-45398 PR_EXT:000001447 denotes E-cadherin
T7353 45446-45459 GO_PATO_EXT:cell_proliferation_or_proliferativity denotes proliferative
T9081 45500-45513 GO:0009653 denotes morphogenesis
T9082 45528-45537 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T9083 45556-45566 UBERON:0000483 denotes epithelium
T9084 45571-45581 UBERON:0003104 denotes mesenchyme
T9085 45582-45588 GO:0065007 denotes govern
T9086 45589-45604 GO_EXT:transcription denotes transcriptional
T9087 45606-45614 GO_PATO_EXT:biological_adhesion_or_adhesiveness denotes adhesive
T9088 45630-45638 GO_EXT:biological_movement_or_translocation_process denotes motility
T9089 45664-45679 UBERON_EXT:cell_clump_or_cluster_or_group_or_mass_or_population denotes groups of cells
T9090 45674-45679 CL_GO_EXT:cell denotes cells
T9091 45693-45700 GO:0005634 denotes nuclear
T9092 45705-45714 GO:0005829 denotes cytosolic
T9093 45781-45786 UBERON:0000062 denotes organ
T9094 45781-45800 GO:0009887 denotes organ morphogenesis
T9095 45984-45989 CL_GO_EXT:cell denotes cells
T9096 46066-46074 CL_GO_EXT:cell denotes cellular
T9097 46066-46074 _FRAGMENT denotes cellular
T9098 46087-46100 GO:0008283 denotes proliferation
T9099 46106-46121 GO:0030154 denotes differentiation
T9100 46208-46212 UBERON:0001037 denotes hair
T9101 46241-46245 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T9464 46248-46257 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes Signaling
T9465 46271-46284 UBERON:0002073 denotes Hair Follicle
T9466 46271-46298 GO:0031069 denotes Hair Follicle Morphogenesis
T9467 46317-46321 UBERON:0001037 denotes hair
T9468 46326-46339 GO:0009653 denotes morphogenesis
T9469 46381-46391 UBERON:0000483 denotes epithelium
T9470 46396-46399 PR_EXT:000000034 denotes BMP
T9471 46439-46449 UBERON:0003104 denotes mesenchyme
T9472 46480-46493 GO_EXT:transcription denotes transcription
T9473 46480-46500 GO_EXT:transcription_factor denotes transcription factor
T9474 46480-46508 GO:0005667 denotes transcription factor complex
T9475 46519-46528 PR_EXT:000002198 denotes β-catenin
T9476 46533-46538 PR_EXT:000009751 denotes LEF-1
T9477 46589-46602 UBERON:0002073 denotes hair follicle
T9478 46625-46639 PR_EXT:000014841 denotes Sonic hedgehog
T9479 46641-46644 PR_EXT:000014841 denotes Shh
T9480 46650-46656 PR_EXT:000000183 denotes TGF-β2
T9481 46657-46666 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T9482 46696-46704 UBERON_EXT:follicle denotes follicle
T9483 46705-46718 GO:0009653 denotes morphogenesis
T9484 46739-46748 PR_EXT:000002198 denotes β-catenin
T9485 46749-46753 SO_EXT:sequence_nullness denotes null
T9486 46754-46758 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T9487 46769-46777 UBERON_EXT:follicle denotes follicle
T9488 46814-46818 UBERON:0001037 denotes hair
T9489 46853-46858 PR_EXT:000009751 denotes LEF-1
T9490 46860-46863 PR_EXT:000014841 denotes Shh
T9491 46868-46874 PR_EXT:000000183 denotes TGF-β2
T9492 46923-46931 UBERON_EXT:follicle denotes follicle
T9493 46939-46949 UBERON:0010512 denotes guard hair
T9494 46951-46964 GO:0009653 denotes morphogenesis
T9495 47019-47024 UBERON:0001037 denotes hairs
T9496 47047-47065 GO:0065007 denotes regulatory control
T9497 47067-47078 UBERON:0010512 denotes Guard hairs
T9498 47102-47107 PR_EXT:000009751 denotes LEF-1
T9499 47112-47118 PR_EXT:000000183 denotes TGF-β2
T9500 47161-47168 GO:0010467 denotes express
T9501 47169-47174 PR_EXT:000015308 denotes Snail
T9502 47235-47245 PR_EXT:000001447 denotes E-cadherin
T9503 47249-47258 GO:0065007 denotes regulated
T9504 47262-47273 UBERON:0010512 denotes guard hairs
T9505 47333-47338 PR_EXT:000015308 denotes Snail
T9506 47362-47366 PR_EXT:000015309 denotes Slug
T9507 47370-47375 PR_EXT:000027825 denotes twist
T9508 47395-47408 GO_EXT:transcription denotes transcription
T9509 47395-47416 GO_EXT:transcription_factor denotes transcription factors
T9510 47427-47436 PR_EXT:000002198 denotes β-catenin
T9511 47467-47472 PR_EXT:000009751 denotes LEF-1
T9512 47477-47480 PR_EXT:000015643 denotes Sry
T9513 47598-47615 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling pathway
T9514 47677-47683 PR_EXT:000000046 denotes TGF-βs
T9515 47719-47732 CL:0000312 denotes keratinocytes
T9516 47742-47746 CL_GO_EXT:cell denotes cell
T9517 47742-47752 GO:0007049 denotes cell cycle
T9518 47771-47777 PR_EXT:000000183 denotes TGF-β2
T9519 47778-47785 CHEBI_PR_EXT:protein denotes protein
T9520 47829-47836 GO:0040007 denotes growing
T9521 47853-47862 _FRAGMENT denotes phases of
T9522 47873-47883 GO:0044851 denotes hair cycle
T9523 47867-47872 UBERON:0007023 denotes adult
T9524 47873-47877 UBERON:0001037 denotes hair
T9525 47975-47981 GO_EXT:biological_growth_entity_or_process denotes growth
T9526 48000-48009 GO:0006915 denotes apoptosis
T9527 48065-48071 PR_EXT:000000182 denotes TGF-β1
T9528 48072-48076 SO_EXT:sequence_nullness denotes null
T9529 48077-48081 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T9530 48110-48117 GO:0040007 denotes growing
T9531 48131-48136 GO:0007567 denotes natal
T9532 48137-48151 UBERON:0002073 denotes hair follicles
T9533 48153-48159 PR_EXT:000000183 denotes TGF-β2
T9534 48160-48164 SO_EXT:sequence_nullness denotes null
T9535 48165-48169 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T9536 48182-48191 UBERON:0000922 denotes embryonic
T9537 48201-48209 UBERON_EXT:follicle denotes follicle
T9538 48275-48281 PR_EXT:000000183 denotes TGF-β2
T9539 48284-48293 UBERON:0000922 denotes embryonic
T9540 48294-48304 GO:0010467 denotes expression
T9541 48333-48339 PR_EXT:000000183 denotes TGF-β2
T9542 48340-48344 SO_EXT:sequence_nullness denotes null
T9543 48388-48394 GO_EXT:biological_growth_entity_or_process denotes growth
T9544 48495-48501 PR_EXT:000000046 denotes TGF-βs
T9545 48520-48526 PR_EXT:000000183 denotes TGF-β2
T9546 48544-48548 PR_EXT:000010425 denotes Ki67
T9547 48549-48559 GO:0010467 denotes expression
T9548 48564-48568 GO_PR_EXT:MAP_kinase denotes MAPK
T9549 48564-48579 GO:0000187 denotes MAPK activation
T9550 48621-48634 UBERON:0002073 denotes hair follicle
T9551 48635-48648 CL:0000312 denotes keratinocytes
T9552 48684-48689 GO_EXT:reaction_or_response denotes react
T9553 48693-48699 PR_EXT:000000183 denotes TGF-β2
T9554 48700-48709 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T9555 48763-48776 PR_EXT:000000046 denotes TGF-β factors
T9556 48863-48872 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes signaling
T9557 48922-48935 GO_PATO_EXT:cell_proliferation_or_proliferativity denotes proliferative
T9558 49013-49017 GO_PR_EXT:SMAD denotes SMAD
T9559 49025-49030 SO_EXT:0000704 denotes genes
T9560 49072-49078 PR_EXT:000000183 denotes TGF-β2
T9561 49082-49095 GO:0008283 denotes proliferation
T9562 49107-49111 UBERON:0001037 denotes hair
T9563 49187-49191 CL_GO_EXT:cell denotes cell
T9564 49271-49284 CL:0000312 denotes keratinocytes
T9565 49329-49334 PR_EXT:000015308 denotes Snail
T9566 49335-49339 SO_EXT:0000704 denotes gene
T9567 49365-49370 PR_EXT:000000046 denotes TGF-β
T9568 49365-49380 GO:0007179 denotes TGF-β signaling
T9569 49429-49434 PR_EXT:000015308 denotes Snail
T9570 49435-49439 SO_EXT:0000704 denotes gene
T9571 49435-49450 GO:0010467 denotes gene expression
T9572 49481-49485 UBERON:0001037 denotes hair
T9573 49526-49536 UBERON:0000483 denotes epithelial
T9574 49542-49555 GO:0008283 denotes proliferation
T9575 49560-49565 PR_EXT:000015308 denotes Snail
T9576 49566-49575 SO_EXT:0000902 denotes transgene
T9577 49576-49586 GO:0010467 denotes expression
T9578 49644-49650 PR_EXT:000000183 denotes TGF-β2
T9579 49655-49660 PR_EXT:000015308 denotes Snail
T9580 49710-49716 PR_EXT:000000183 denotes TGF-β2
T9581 49718-49723 PR_EXT:000015308 denotes Snail
T9582 49724-49734 GO:0010467 denotes expression
T9583 49755-49761 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T9584 49762-49766 UBERON:0001037 denotes hair
T9585 49792-49795 PR_EXT:000009454 denotes K14
T9586 49796-49801 PR_EXT:000000364 denotes Smad2
T9587 49802-49806 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T9588 49808-49813 PR_EXT:000015308 denotes Snail
T9589 49900-49906 PR_EXT:000000183 denotes TGF-β2
T9590 49956-49961 PR_EXT:000015308 denotes Snail
T9591 50005-50010 PR_EXT:000015308 denotes Snail
T9592 50025-50034 GO:0010467 denotes expressed
T9593 50042-50055 UBERON:0002073 denotes hair follicle
T9594 50042-50069 GO:0031069 denotes hair follicle morphogenesis
T9595 50126-50136 GO:0010467 denotes expression
T9596 50140-50142 SO_EXT:transgenic_entity denotes Tg
T9597 50143-50148 PR_EXT:000015308 denotes Snail
T9598 50156-50161 GO:0007567 denotes natal
T9599 50162-50166 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T9600 50197-50208 CHEBI_EXT:biochemical denotes biochemical
T9601 50218-50228 UBERON:0000483 denotes epithelial
T9602 50218-50255 GO:0001837 denotes epithelial to mesenchymal transitions
T9603 50232-50243 UBERON:0003104 denotes mesenchymal
T9604 50303-50308 PR_EXT:000015308 denotes Snail
T9605 50309-50319 GO:0010467 denotes expression
T9606 50354-50367 UBERON:0002073 denotes hair follicle
T9607 50354-50381 GO:0031069 denotes hair follicle morphogenesis
T9608 50421-50430 UBERON:0010166 denotes hair coat
T9609 50434-50437 PR_EXT:000009454 denotes K14
T9610 50438-50443 PR_EXT:000015308 denotes Snail
T9611 50444-50446 SO_EXT:transgenic_entity denotes Tg
T9612 50447-50451 NCBITaxon:10088 denotes mice
T9613 50485-50493 UBERON_EXT:follicle denotes follicle
T9614 50571-50576 UBERON:0001037 denotes hairs
T9615 50607-50609 SO_EXT:transgenic_entity denotes Tg
T9616 50610-50619 UBERON:0007376 denotes epidermis
T9617 50676-50684 UBERON_EXT:follicle denotes follicle
T9618 50706-50710 NCBITaxon:10088 denotes mice
T9619 50738-50741 PR_EXT:000009454 denotes K14
T9620 50742-50750 SO_EXT:0000167 denotes promoter
T9621 50777-50791 _FRAGMENT denotes basal layer of
T9622 50796-50805 UBERON:0002025 denotes epidermis
T9623 50814-50831 _FRAGMENT denotes outer root sheath
T9624 50838-50840 _FRAGMENT denotes of
T9625 50845-50858 UBERON:0005942 denotes hair follicle
T9626 50833-50836 _FRAGMENT denotes ORS
T9627 50876-50890 GO_EXT:negative_regulation denotes down-regulated
T9628 50905-50914 UBERON:0000922 denotes embryonic
T9629 50915-50919 UBERON:0001037 denotes hair
T9630 50947-50952 GO:0007567 denotes natal
T9631 50953-50957 UBERON:0001037 denotes hair
T9632 50969-50974 CL_GO_EXT:cell denotes cells
T9633 50980-50983 PR_EXT:000009454 denotes K14
T9634 50984-50992 SO_EXT:0000167 denotes promoter
T9635 51020-51023 UBERON:0005942 denotes ORS
T9636 51029-51038 UBERON:0007376 denotes epidermis
T9637 51098-51106 GO_EXT:reaction_or_response denotes response
T9638 51114-51117 UBERON:0005942 denotes ORS
T9639 51129-51134 PR_EXT:000015308 denotes Snail
T9640 51197-51202 PR_EXT:000015308 denotes Snail
T9641 51241-51251 GO:0010467 denotes expression
T9642 51289-51299 GO:0065007 denotes regulatory
T9643 51316-51322 GO:0065007 denotes govern
T9644 51323-51325 GO:0005912 denotes AJ
T9645 51323-51335 GO:0034333 denotes AJ formation
T9646 51337-51346 GO_EXT:biological_movement_or_translocation_process denotes migration
T9647 51352-51365 GO:0008283 denotes proliferation
T9648 51373-51385 UBERON:0005942 denotes follicle ORS
T9649 51459-51463 NCBITaxon:10088 denotes mice
T9650 51474-51478 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T9651 51505-51510 PR_EXT:000015308 denotes Snail
T9652 51511-51520 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T10897 51537-51546 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes Signaling
T10898 51548-51563 GO_EXT:transcription denotes Transcriptional
T10899 51574-51584 UBERON:0000483 denotes Epithelial
T10900 51601-51614 GO:0008283 denotes Proliferation
T10901 51622-51626 UBERON:0001037 denotes Hair
T10902 51675-51688 UBERON:0002073 denotes hair follicle
T10903 51675-51702 GO:0031069 denotes hair follicle morphogenesis
T10904 51704-51714 PR_EXT:000001447 denotes E-cadherin
T10905 51715-51719 SO_EXT:0000704 denotes gene
T10906 51715-51730 GO:0010467 denotes gene expression
T10907 51734-51748 GO_EXT:negative_regulation denotes down-regulated
T10908 51803-51808 CL_GO_EXT:cell denotes cells
T10909 51818-51822 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T10910 51897-51902 PR_EXT:000009751 denotes LEF-1
T10911 51903-51912 PR_EXT:000002198 denotes β-catenin
T10912 51913-51926 GO_EXT:transcription denotes transcription
T10913 51913-51933 GO_EXT:transcription_factor denotes transcription factor
T10914 51913-51941 GO:0005667 denotes transcription factor complex
T10915 51996-52001 PR_EXT:000009751 denotes LEF-1
T10916 52006-52013 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T10917 52006-52018 SO_EXT:0000409 denotes binding site
T10918 52026-52036 PR_EXT:000001447 denotes E-cadherin
T10919 52037-52045 SO_EXT:0000167 denotes promoter
T10920 52122-52127 PR_EXT:000009751 denotes LEF-1
T10921 52128-52137 PR_EXT:000002198 denotes β-catenin
T10922 52170-52180 PR_EXT:000001447 denotes E-cadherin
T10923 52181-52185 SO_EXT:0000704 denotes gene
T10924 52181-52196 GO:0010467 denotes gene expression
T10925 52200-52204 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T10926 52205-52218 CL:0000312 denotes keratinocytes
T10927 52275-52280 PR_EXT:000015308 denotes Snail
T10928 52320-52333 CL:0000312 denotes keratinocytes
T10929 52382-52387 PR_EXT:000015308 denotes Snail
T10930 52391-52400 GO:0010467 denotes expressed
T10931 52435-52450 GO_PATO_EXT:physiological_process_or_quality denotes physiologically
T10932 52479-52485 PR_EXT:000000021 denotes noggin
T10933 52486-52490 SO_EXT:sequence_nullness denotes null
T10934 52491-52500 UBERON:0000922 denotes embryonic
T10935 52501-52505 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T10936 52507-52512 PR_EXT:000009751 denotes LEF-1
T10937 52513-52523 GO:0010467 denotes expression
T10938 52557-52562 PR_EXT:000009751 denotes LEF-1
T10939 52563-52572 PR_EXT:000002198 denotes β-catenin
T10940 52582-52586 SO_EXT:0000704 denotes gene
T10941 52618-52626 UBERON_EXT:placode denotes placodes
T10942 52657-52667 PR_EXT:000001447 denotes E-cadherin
T10943 52668-52683 GO_EXT:negative_regulation denotes down-regulation
T10944 52714-52717 _FRAGMENT denotes Wnt
T10945 52725-52734 GO:0016055 denotes signaling
T10946 52718-52724 PR_EXT:000000021 denotes noggin
T10947 52738-52748 GO:0065007 denotes regulating
T10948 52763-52771 UBERON_EXT:follicle denotes follicle
T10949 52772-52785 GO:0009653 denotes morphogenesis
T10950 52803-52807 SO_EXT:0000704 denotes gene
T10951 52865-52870 PR_EXT:000015308 denotes Snail
T10952 52909-52927 _FRAGMENT denotes down-regulation in
T10953 52939-52954 GO:0010629 denotes gene expression
T10954 52928-52938 PR_EXT:000001447 denotes E-cadherin
T10955 52939-52943 SO_EXT:0000704 denotes gene
T10956 52974-52982 UBERON_EXT:follicle denotes follicle
T10957 53025-53028 PR_EXT:000009454 denotes K14
T10958 53029-53034 PR_EXT:000015308 denotes Snail
T10959 53035-53037 SO_EXT:transgenic_entity denotes Tg
T10960 53038-53047 UBERON:0007376 denotes epidermis
T10961 53067-53085 _FRAGMENT denotes down-regulation in
T10962 53097-53107 GO:0010629 denotes expression
T10963 53086-53096 PR_EXT:000001447 denotes E-cadherin
T10964 53157-53161 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T10965 53207-53217 PR_EXT:000001447 denotes E-cadherin
T10966 53254-53263 PR_EXT:alpha_catenin denotes α-catenin
T10967 53265-53278 UBERON:0002073 denotes hair follicle
T10968 53265-53292 GO:0031069 denotes hair follicle morphogenesis
T10969 53327-53336 UBERON:0007376 denotes epidermal
T10970 53342-53355 GO:0008283 denotes proliferation
T10971 53368-53371 PR_EXT:000009454 denotes K14
T10972 53372-53377 PR_EXT:000015308 denotes Snail
T10973 53378-53380 SO_EXT:transgenic_entity denotes Tg
T10974 53381-53385 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T10975 53428-53441 GO:0008283 denotes proliferation
T10976 53446-53451 PR_EXT:000015308 denotes Snail
T10977 53455-53461 PR_EXT:000000183 denotes TGF-β2
T10978 53462-53466 SO_EXT:sequence_nullness denotes null
T10979 53467-53471 UBERON:0001037 denotes hair
T10980 53506-53521 GO_EXT:negative_regulation denotes down-regulation
T10981 53525-53535 PR_EXT:000001447 denotes E-cadherin
T10982 53543-53551 UBERON_EXT:follicle denotes follicle
T10983 53552-53565 GO:0009653 denotes morphogenesis
T10984 53610-53623 GO_PATO_EXT:cell_proliferation_or_proliferativity denotes proliferative
T10985 53639-53644 CL_GO_EXT:cell denotes cells
T10986 53650-53652 SO_EXT:transgenic_entity denotes Tg
T10987 53706-53716 GO:0065007 denotes regulation
T10988 53720-53730 PR_EXT:000001447 denotes E-cadherin
T10989 53732-53737 PR_EXT:000015308 denotes Snail
T10990 53763-53774 GO_UBERON_EXT:cellular_component_or_cell_part denotes subcellular
T10991 53766-53774 CL_GO_EXT:cell denotes cellular
T10992 53775-53787 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T10993 53804-53806 GO:0005912 denotes AJ
T10994 53818-53826 CHEBI_PR_EXT:protein denotes proteins
T10995 53855-53860 PR_EXT:000009231 denotes Ajuba
T10996 53918-53922 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes bind
T10997 53923-53928 PR_EXT:000008224 denotes Grb-2
T10998 53940-53949 PR_EXT:alpha_catenin denotes α-catenin
T10999 53987-53991 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T11000 53992-54005 CL:0000312 denotes keratinocytes
T11001 54039-54043 SO_EXT:sequence_nullness denotes null
T11002 54044-54052 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T11003 54056-54065 PR_EXT:alpha_catenin denotes α-catenin
T11004 54078-54085 GO:0010467 denotes express
T11005 54086-54091 PR_EXT:000015308 denotes Snail
T11006 54093-54098 PR_EXT:000009231 denotes Ajuba
T11007 54128-54133 PR_EXT:000008224 denotes Grb-2
T11008 54168-54170 SO_EXT:wild_type_entity_or_quality denotes WT
T11009 54171-54184 CL:0000312 denotes keratinocytes
T11010 54224-54229 PR_EXT:000015308 denotes Snail
T11011 54230-54241 GO:0009294 denotes transfected
T11012 54245-54250 PR_EXT:000009231 denotes Ajuba
T11013 54251-54262 GO:0009294 denotes transfected
T11014 54263-54276 CL:0000312 denotes keratinocytes
T11015 54319-54323 GO_PR_EXT:MAP_kinase denotes MAPK
T11016 54349-54354 PR_EXT:000008224 denotes Grb-2
T11017 54370-54375 PR_EXT:000009231 denotes Ajuba
T11018 54414-54416 GO:0005912 denotes AJ
T11019 54417-54425 CHEBI_PR_EXT:protein denotes proteins
T11020 54475-54488 GO:0008283 denotes proliferation
T11021 54500-54510 UBERON:0000483 denotes epithelial
T11022 54500-54510 _FRAGMENT denotes epithelial
T11023 54554-54558 CL:0000066 denotes cell
T11024 54530-54536 NCBITaxon:9608 denotes canine
T11025 54537-54543 UBERON:0002113 denotes kidney
T11026 54554-54558 CL_GO_EXT:cell denotes cell
T11027 54566-54571 PR_EXT:000015308 denotes Snail
T11028 54596-54600 CL_GO_EXT:cell denotes cell
T11029 54596-54606 GO:0007049 denotes cell cycle
T11030 54631-54639 GO_EXT:biological_movement_or_translocation_process denotes motility
T11031 54734-54739 PR_EXT:000015308 denotes Snail
T11032 54743-54751 GO_EXT:biological_movement_or_translocation_process denotes motility
T11033 54756-54766 UBERON:0000483 denotes epithelial
T11034 54807-54812 PR_EXT:000015308 denotes Snail
T11035 54824-54837 GO_PATO_EXT:cell_proliferation_or_proliferativity denotes proliferative
T11036 54903-54911 GO_EXT:reaction_or_response denotes response
T11037 54925-54929 CL_GO_EXT:cell denotes cell
T11038 54939-54944 PR_EXT:000015308 denotes Snail
T11039 54945-54955 GO:0010467 denotes expression
T11040 55030-55035 NCBITaxon:10088 denotes mouse
T11041 55098-55102 CL_GO_EXT:cell denotes cell
T11042 55314-55329 GO_EXT:negative_regulation denotes down-regulation
T11043 55333-55335 GO:0005912 denotes AJ
T11044 55336-55344 CHEBI_PR_EXT:protein denotes proteins
T11045 55369-55382 GO:0008283 denotes proliferation
T11046 55459-55469 UBERON:0000483 denotes epithelial
T11047 55470-55483 GO:0008283 denotes proliferation
T11048 55489-55504 GO_EXT:negative_regulation denotes down-regulation
T11049 55508-55510 GO:0005912 denotes AJ
T11050 55511-55519 CHEBI_PR_EXT:protein denotes proteins
T11051 55708-55712 CL_GO_EXT:cell denotes cell
T11052 55800-55808 CL_GO_EXT:cell denotes cellular
T11053 55833-55846 GO:0008283 denotes proliferation
T11054 55850-55860 UBERON:0000483 denotes epithelial
T11055 55850-55874 GO:0002009 denotes epithelial morphogenesis
T11056 55930-55939 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T11973 56104-56112 CHEBI_EXT:33893 denotes Reagents
T11974 56121-56131 GO:0042571 denotes antibodies
T11975 56151-56161 PR_EXT:000001447 denotes E-cadherin
T11976 56202-56211 PR_EXT:alpha_catenin denotes α-catenin
T11977 56213-56222 PR_EXT:000002198 denotes β-catenin
T11978 56231-56238 PR_EXT:000028799 denotes tubulin
T11979 56284-56289 PR_EXT:000009231 denotes Ajuba
T11980 56364-56375 PR_EXT:000009140 denotes β4 integrin
T11981 56367-56375 GO_PR_EXT:integrin denotes integrin
T11982 56376-56381 PR_EXT:000009140 denotes CD104
T11983 56437-56446 GO:0005610 denotes laminin 5
T11984 56523-56525 PR_EXT:000009481 denotes K5
T11985 56527-56529 PR_EXT:000009450 denotes K1
T11986 56531-56539 PR_EXT:000009882 denotes loricrin
T11987 56553-56563 PR_EXT:000009167 denotes involucrin
T11988 56565-56574 PR_EXT:000007551 denotes fillagrin
T11989 56623-56627 GO_PR_EXT:MAP_kinase denotes MAPK
T11990 56637-56641 CL_GO_EXT:cell denotes Cell
T11991 56642-56651 GO_EXT:cell_communication_or_signaling_or_signal_transduction denotes Signaling
T11992 56693-56698 PR_EXT:000008224 denotes Grb-2
T11993 56760-56770 PR_EXT:000005245 denotes P-cadherin
T11994 56851-56863 CHEBI_EXT:biochemical denotes Biochemicals
T11995 56866-56874 PR_EXT:000017298 denotes vimentin
T11996 56924-56928 PR_EXT:000010425 denotes Ki67
T11997 56981-56990 PR_EXT:keratin_6 denotes keratin 6
T11998 57067-57075 PR_EXT:cyclin_D denotes cyclin D
T11999 57130-57136 PR_EXT:000000183 denotes TGF-β2
T12000 57204-57208 CHEBI:37926 denotes FITC
T12001 57211-57220 CHEBI:51247 denotes Texas Red
T12002 57230-57240 MOP:0000779 denotes conjugated
T12003 57251-57261 GO:0042571 denotes antibodies
T12004 57338-57350 MOP:0000093 denotes Biotinylated
T12005 57361-57371 GO:0042571 denotes antibodies
T12006 57502-57507 PR_EXT:000015308 denotes Snail
T12007 57508-57516 GO:0042571 denotes antibody
T12008 57534-57545 NCBITaxon:10140 denotes Guinea pigs
T12009 57575-57585 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminal
T12010 57586-57594 SO_EXT:biological_sequence denotes sequence
T12011 57598-57604 NCBITaxon:39107 denotes murine
T12012 57605-57610 PR_EXT:000015308 denotes Snail
T12013 57611-57616 SO_EXT:sequence_fusion_process denotes fused
T12014 57620-57623 GO_PR_EXT:glutathione_S_transferase denotes GST
T12015 57673-57684 GO_SO_EXT:sequence_rearrangement_process denotes Recombinant
T12016 57685-57690 NCBITaxon:9606 denotes human
T12017 57691-57697 PR_EXT:000000183 denotes TGF-β2
T12018 57779-57785 PR_EXT:000000183 denotes TGF-β2
T12019 57815-57826 GO_SO_EXT:sequence_rearrangement_process denotes recombinant
T12020 57827-57834 CHEBI_PR_EXT:protein denotes protein
T804 3260-3273 _FRAGMENT denotes proliferation
T7042 34129-34139 GO:0065007 denotes regulating
T7299 44158-44162 UBERON:0001037 denotes hair
T7300 44187-44191 PR_EXT:000010425 denotes Ki67
T7301 44249-44255 PR_EXT:000000183 denotes TGF-β2
T7302 44267-44272 PR_EXT:000015308 denotes Snail
T7303 44273-44283 GO:0010467 denotes expression
T7304 44317-44326 GO_EXT:0016015 denotes morphogen
T7305 44359-44369 GO:0010467 denotes expression
T7306 44373-44378 SO_EXT:0000704 denotes genes
T7307 44411-44416 PR_EXT:000015308 denotes Snail
T7308 44443-44448 PR_EXT:000015308 denotes Snail
T7309 44476-44486 PR_EXT:000001447 denotes E-cadherin
T7310 44487-44491 SO_EXT:0000704 denotes gene
T7311 44531-44541 PR_EXT:000001447 denotes E-cadherin
T7312 44545-44551 PR_EXT:000000183 denotes TGF-β2
T7313 44552-44556 SO_EXT:sequence_nullness denotes null
T7314 44586-44590 UBERON:0001037 denotes hair
T7315 44599-44605 PR_EXT:000000183 denotes TGF-β2
T7316 44606-44610 SO_EXT:sequence_nullness denotes null
T7317 44611-44615 UBERON_EXT:zone_of_skin_or_skin_of_body denotes skin
T7318 44681-44683 SO_EXT:wild_type_entity_or_quality denotes WT
T7319 44758-44771 GO:0005576 denotes extracellular
T7320 44763-44771 CL_GO_EXT:cell denotes cellular
T7321 44788-44794 PR_EXT:000000021 denotes noggin
T7322 44816-44829 _FRAGMENT denotes generation of
T7323 44862-44869 GO:0065003 denotes complex
T7324 44832-44837 PR_EXT:000009751 denotes LEF-1
T7325 44838-44847 PR_EXT:000002198 denotes β-catenin
T7326 44848-44861 GO_EXT:transcription denotes transcription
T7327 44862-44869 GO:0032991 denotes complex
T7328 44881-44891 PR_EXT:000001447 denotes E-cadherin
T7334 45016-45023 GO:0005634 denotes nuclear
R1427 T2627 T2626 _lexicallyChainedTo to,cells
R1428 T2628 T2627 _lexicallyChainedTo cell fate,to
R1429 T2631 T2630 _lexicallyChainedTo ectoderm,cells of
R1430 T2641 T2640 _lexicallyChainedTo follicles,hair
R1431 T2658 T2657 _lexicallyChainedTo cells,nuclei of
R1432 T2700 T2699 _lexicallyChainedTo expression,down-regulation in
R2288 T3861 T3860 _lexicallyChainedTo spinous,layers
R2876 T5077 T5076 _lexicallyChainedTo to,translocation
R2877 T5078 T5077 _lexicallyChainedTo nucleus,to
R4165 T7032 T7031 _lexicallyChainedTo Gene Expression,Regulation of
R4166 T7060 T7059 _lexicallyChainedTo signaling,Wnt
R4167 T7066 T7065 _lexicallyChainedTo signal,transmission of
R4168 T7110 T7109 _lexicallyChainedTo epidermis,basal layer of
R4169 T7159 T7158 _lexicallyChainedTo gene,site of
R4170 T7323 T7322 _lexicallyChainedTo complex,generation of
R4171 T7339 T7338 _lexicallyChainedTo signaling pathway,Wnt
R5770 T9098 T9097 _lexicallyChainedTo proliferation,cellular
R5771 T9099 T9097 _lexicallyChainedTo differentiation,cellular
R5890 T9522 T9521 _lexicallyChainedTo hair cycle,phases of
R5891 T9622 T9621 _lexicallyChainedTo epidermis,basal layer of
R5892 T9624 T9623 _lexicallyChainedTo of,outer root sheath
R5893 T9624 T9626 _lexicallyChainedTo of,ORS
R5894 T9625 T9624 _lexicallyChainedTo hair follicle,of
R6872 T10945 T10944 _lexicallyChainedTo signaling,Wnt
R6873 T10953 T10952 _lexicallyChainedTo gene expression,down-regulation in
R6874 T10962 T10961 _lexicallyChainedTo expression,down-regulation in
R6875 T11023 T11022 _lexicallyChainedTo cell,epithelial
R8873 T13629 T13628 _lexicallyChainedTo UTR,region of
R220 T749 T748 _lexicallyChainedTo organs,formation of
R221 T805 T804 _lexicallyChainedTo of,proliferation
R222 T806 T805 _lexicallyChainedTo cells,of
R223 T814 T813 _lexicallyChainedTo cell fates,determination of
R224 T832 T831 _lexicallyChainedTo cells,cluster of
R225 T838 T837 _lexicallyChainedTo cells,group of
R226 T949 T948 _lexicallyChainedTo cells,motile
R1425 T2569 T2568 _lexicallyChainedTo UTR,3′
R1426 T2626 T2625 _lexicallyChainedTo cells,commitment of
R1 T134 T133 _lexicallyChainedTo cells,cell

craft-sa-dev

Id Subject Object Predicate Lexical cue
T12464 57858-57862 NNS denotes Mice
T12465 57862-58065 sentence denotes The K14-Snail Tg mouse was generated by digesting the pcDNA3-mm Snail-HA plasmid (G. de Herreros, Universitat Pompeu, Fabra, Barcelona, Spain) with BamHI and NotI and subcloned into the K14 vector [49].
T12466 57863-57866 DT denotes The
T12467 57880-57885 NN denotes mouse
T12468 57867-57870 NN denotes K14
T12469 57871-57876 NN denotes Snail
T12470 57870-57871 HYPH denotes -
T12471 57877-57879 NN denotes Tg
T12472 57890-57899 VBN denotes generated
T12473 57886-57889 VBD denotes was
T12474 57900-57902 IN denotes by
T12475 57903-57912 VBG denotes digesting
T12476 57913-57916 DT denotes the
T12477 57936-57943 NN denotes plasmid
T12478 57917-57923 NN denotes pcDNA3
T12479 57924-57926 NN denotes mm
T12480 57923-57924 HYPH denotes -
T12481 57927-57932 NN denotes Snail
T12482 57933-57935 NN denotes HA
T12483 57932-57933 HYPH denotes -
T12484 57944-57945 -LRB- denotes (
T12485 57951-57959 NNP denotes Herreros
T12486 57945-57947 NNP denotes G.
T12487 57948-57950 NNP denotes de
T12488 57959-57961 , denotes ,
T12489 57961-57972 NNP denotes Universitat
T12490 57973-57979 NNP denotes Pompeu
T12491 57979-57981 , denotes ,
T12492 57981-57986 NNP denotes Fabra
T12493 57986-57988 , denotes ,
T12494 57988-57997 NNP denotes Barcelona
T12495 57997-57999 , denotes ,
T12496 57999-58004 NNP denotes Spain
T12497 58004-58005 -RRB- denotes )
T12498 58006-58010 IN denotes with
T12499 58011-58016 NN denotes BamHI
T12500 58017-58020 CC denotes and
T12501 58021-58025 NN denotes NotI
T12502 58026-58029 CC denotes and
T12503 58030-58039 VBN denotes subcloned
T12504 58040-58044 IN denotes into
T12505 58045-58048 DT denotes the
T12506 58053-58059 NN denotes vector
T12507 58049-58052 NN denotes K14
T12508 58060-58061 -LRB- denotes [
T12509 58061-58063 CD denotes 49
T12510 58063-58064 -RRB- denotes ]
T12511 58064-58065 . denotes .
T12512 58065-58146 sentence denotes The linearized construct was injected into the nucleus of embryos from CD1 mice.
T12513 58066-58069 DT denotes The
T12514 58081-58090 NN denotes construct
T12515 58070-58080 VBN denotes linearized
T12516 58095-58103 VBN denotes injected
T12517 58091-58094 VBD denotes was
T12518 58104-58108 IN denotes into
T12519 58109-58112 DT denotes the
T12520 58113-58120 NN denotes nucleus
T12521 58121-58123 IN denotes of
T12522 58124-58131 NNS denotes embryos
T12523 58132-58136 IN denotes from
T12524 58137-58140 NN denotes CD1
T12525 58141-58145 NNS denotes mice
T12526 58145-58146 . denotes .
T12527 58146-58204 sentence denotes The K14-Smad 2 Tg mouse was reported in Ito et al., 2001.
T12528 58147-58150 DT denotes The
T12529 58165-58170 NN denotes mouse
T12530 58151-58154 NN denotes K14
T12531 58155-58159 NN denotes Smad
T12532 58154-58155 HYPH denotes -
T12533 58160-58161 CD denotes 2
T12534 58162-58164 NN denotes Tg
T12535 58175-58183 VBN denotes reported
T12536 58171-58174 VBD denotes was
T12537 58184-58186 IN denotes in
T12538 58187-58190 NNP denotes Ito
T12539 58191-58193 FW denotes et
T12540 58194-58197 FW denotes al.
T12541 58197-58199 , denotes ,
T12542 58199-58203 CD denotes 2001
T12543 58203-58204 . denotes .
T12544 58204-58258 sentence denotes The TGF-β2 knockout (KO) mouse was described in [34].
T12545 58205-58208 DT denotes The
T12546 58230-58235 NN denotes mouse
T12547 58209-58212 NN denotes TGF
T12548 58213-58215 NN denotes β2
T12549 58212-58213 HYPH denotes -
T12550 58216-58224 NN denotes knockout
T12551 58225-58226 -LRB- denotes (
T12552 58226-58228 NN denotes KO
T12553 58228-58229 -RRB- denotes )
T12554 58240-58249 VBN denotes described
T12555 58236-58239 VBD denotes was
T12556 58250-58252 IN denotes in
T12557 58253-58254 -LRB- denotes [
T12558 58254-58256 NN denotes 34
T12559 58256-58257 -RRB- denotes ]
T12560 58257-58258 . denotes .
T12561 58258-58333 sentence denotes The shh KO mouse [38] and TOPGal mouse [20] have previously been reported.
T12562 58259-58262 DT denotes The
T12563 58270-58275 NN denotes mouse
T12564 58263-58266 NN denotes shh
T12565 58267-58269 NN denotes KO
T12566 58324-58332 VBN denotes reported
T12567 58276-58277 -LRB- denotes [
T12568 58277-58279 CD denotes 38
T12569 58279-58280 -RRB- denotes ]
T12570 58281-58284 CC denotes and
T12571 58285-58291 NN denotes TOPGal
T12572 58292-58297 NN denotes mouse
T12573 58298-58299 -LRB- denotes [
T12574 58299-58301 CD denotes 20
T12575 58301-58302 -RRB- denotes ]
T12576 58303-58307 VBP denotes have
T12577 58308-58318 RB denotes previously
T12578 58319-58323 VBN denotes been
T12579 58332-58333 . denotes .
T12687 58335-58342 NNP denotes Western
T12688 58343-58347 NN denotes blot
T12689 58348-58351 CC denotes and
T12690 58352-58371 NN denotes immunoprecipitation
T12691 58371-58677 sentence denotes Protein extracts from primary keratinocytes were generated either by lysing cells in lysis buffer (1% NP-40, 1% sodium deoxycholate, 20 mM Tris-Cl [pH 7.4], 140 mM NaCl containing 1 mM sodium vanadate, 2 mM phenylmethylsulfonyl fluoride, and protease inhibitors) or directly in Laemmli bβuffer and boiled.
T12692 58372-58379 NN denotes Protein
T12693 58380-58388 NNS denotes extracts
T12694 58421-58430 VBN denotes generated
T12695 58389-58393 IN denotes from
T12696 58394-58401 JJ denotes primary
T12697 58402-58415 NNS denotes keratinocytes
T12698 58416-58420 VBD denotes were
T12699 58431-58437 CC denotes either
T12700 58438-58440 IN denotes by
T12701 58441-58447 VBG denotes lysing
T12702 58448-58453 NNS denotes cells
T12703 58454-58456 IN denotes in
T12704 58457-58462 NN denotes lysis
T12705 58463-58469 NN denotes buffer
T12706 58470-58471 -LRB- denotes (
T12707 58474-58476 NN denotes NP
T12708 58471-58472 CD denotes 1
T12709 58472-58473 NN denotes %
T12710 58476-58477 HYPH denotes -
T12711 58477-58479 CD denotes 40
T12712 58479-58481 , denotes ,
T12713 58481-58482 CD denotes 1
T12714 58482-58483 NN denotes %
T12715 58491-58503 NN denotes deoxycholate
T12716 58484-58490 NN denotes sodium
T12717 58503-58505 , denotes ,
T12718 58505-58507 CD denotes 20
T12719 58508-58510 NN denotes mM
T12720 58516-58518 NN denotes Cl
T12721 58511-58515 NN denotes Tris
T12722 58515-58516 HYPH denotes -
T12723 58519-58520 -LRB- denotes [
T12724 58520-58522 NN denotes pH
T12725 58523-58526 CD denotes 7.4
T12726 58526-58527 -RRB- denotes ]
T12727 58527-58529 , denotes ,
T12728 58529-58532 CD denotes 140
T12729 58533-58535 NN denotes mM
T12730 58536-58540 NN denotes NaCl
T12731 58541-58551 VBG denotes containing
T12732 58552-58553 CD denotes 1
T12733 58554-58556 NN denotes mM
T12734 58564-58572 NN denotes vanadate
T12735 58557-58563 NN denotes sodium
T12736 58572-58574 , denotes ,
T12737 58574-58575 CD denotes 2
T12738 58576-58578 NN denotes mM
T12739 58600-58608 NN denotes fluoride
T12740 58579-58599 NN denotes phenylmethylsulfonyl
T12741 58608-58610 , denotes ,
T12742 58610-58613 CC denotes and
T12743 58614-58622 NN denotes protease
T12744 58623-58633 NNS denotes inhibitors
T12745 58633-58634 -RRB- denotes )
T12746 58635-58637 CC denotes or
T12747 58638-58646 RB denotes directly
T12748 58647-58649 IN denotes in
T12749 58650-58657 NNP denotes Laemmli
T12750 58658-58665 NN denotes bβuffer
T12751 58666-58669 CC denotes and
T12752 58670-58676 VBN denotes boiled
T12753 58676-58677 . denotes .
T12754 58677-58826 sentence denotes For skin tissue: Frozen tissue was pulverized in a liquid nitrogen-cooled Gevebesmascher and the powder scraped into a chilled microcentrifuge tube.
T12755 58678-58681 IN denotes For
T12756 58713-58723 VBN denotes pulverized
T12757 58682-58686 NN denotes skin
T12758 58687-58693 NN denotes tissue
T12759 58693-58695 : denotes :
T12760 58695-58701 JJ denotes Frozen
T12761 58702-58708 NN denotes tissue
T12762 58709-58712 VBD denotes was
T12763 58724-58726 IN denotes in
T12764 58727-58728 DT denotes a
T12765 58752-58766 NN denotes Gevebesmascher
T12766 58729-58735 JJ denotes liquid
T12767 58736-58744 NN denotes nitrogen
T12768 58745-58751 VBN denotes cooled
T12769 58744-58745 HYPH denotes -
T12770 58767-58770 CC denotes and
T12771 58771-58774 DT denotes the
T12772 58775-58781 NN denotes powder
T12773 58782-58789 VBN denotes scraped
T12774 58790-58794 IN denotes into
T12775 58795-58796 DT denotes a
T12776 58821-58825 NN denotes tube
T12777 58797-58804 JJ denotes chilled
T12778 58805-58820 NN denotes microcentrifuge
T12779 58825-58826 . denotes .
T12780 58826-58983 sentence denotes RIPA buffer (1% Triton X-100 in PBS with 10 mM EDTA, 150 mN NaCl, 1% sodium deoxycholate, and 0.1% SDS) and protease inhibitors or Laemmli buffer was added.
T12781 58827-58831 NN denotes RIPA
T12782 58832-58838 NN denotes buffer
T12783 58977-58982 VBN denotes added
T12784 58839-58840 -LRB- denotes (
T12785 58850-58851 NN denotes X
T12786 58840-58841 CD denotes 1
T12787 58841-58842 NN denotes %
T12788 58843-58849 NN denotes Triton
T12789 58851-58852 HYPH denotes -
T12790 58852-58855 CD denotes 100
T12791 58856-58858 IN denotes in
T12792 58859-58862 NN denotes PBS
T12793 58863-58867 IN denotes with
T12794 58868-58870 CD denotes 10
T12795 58871-58873 NN denotes mM
T12796 58874-58878 NN denotes EDTA
T12797 58878-58880 , denotes ,
T12798 58880-58883 CD denotes 150
T12799 58884-58886 NN denotes mN
T12800 58887-58891 NN denotes NaCl
T12801 58891-58893 , denotes ,
T12802 58893-58894 CD denotes 1
T12803 58894-58895 NN denotes %
T12804 58903-58915 NN denotes deoxycholate
T12805 58896-58902 NN denotes sodium
T12806 58915-58917 , denotes ,
T12807 58917-58920 CC denotes and
T12808 58921-58924 CD denotes 0.1
T12809 58924-58925 NN denotes %
T12810 58926-58929 NN denotes SDS
T12811 58929-58930 -RRB- denotes )
T12812 58931-58934 CC denotes and
T12813 58935-58943 NN denotes protease
T12814 58944-58954 NNS denotes inhibitors
T12815 58955-58957 CC denotes or
T12816 58958-58965 NNP denotes Laemmli
T12817 58966-58972 NN denotes buffer
T12818 58973-58976 VBD denotes was
T12819 58982-58983 . denotes .
T12820 58983-59077 sentence denotes The cell suspension was sonicated three times for 15 s and centrifuged at 14,000 rpm at 4 °C.
T12821 58984-58987 DT denotes The
T12822 58993-59003 NN denotes suspension
T12823 58988-58992 NN denotes cell
T12824 59008-59017 VBN denotes sonicated
T12825 59004-59007 VBD denotes was
T12826 59018-59023 CD denotes three
T12827 59024-59029 NNS denotes times
T12828 59030-59033 IN denotes for
T12829 59034-59036 CD denotes 15
T12830 59037-59038 NN denotes s
T12831 59039-59042 CC denotes and
T12832 59043-59054 VBN denotes centrifuged
T12833 59055-59057 IN denotes at
T12834 59058-59064 CD denotes 14,000
T12835 59065-59068 NN denotes rpm
T12836 59069-59071 IN denotes at
T12837 59072-59073 CD denotes 4
T12838 59074-59076 NN denotes °C
T12839 59076-59077 . denotes .
T12840 59077-59152 sentence denotes The supernatant was separated from the pellet and used in the experiments.
T12841 59078-59081 DT denotes The
T12842 59082-59093 NN denotes supernatant
T12843 59098-59107 VBN denotes separated
T12844 59094-59097 VBD denotes was
T12845 59108-59112 IN denotes from
T12846 59113-59116 DT denotes the
T12847 59117-59123 NN denotes pellet
T12848 59124-59127 CC denotes and
T12849 59128-59132 VBN denotes used
T12850 59133-59135 IN denotes in
T12851 59136-59139 DT denotes the
T12852 59140-59151 NNS denotes experiments
T12853 59151-59152 . denotes .
T12854 59152-59343 sentence denotes Extracts subjected to immunoprecipitation were precleared with Protein G Sepharose (Amersham, Piscataway, New York, United States) and incubated with antibody with rocking overnight at 4 °C.
T12855 59153-59161 NNS denotes Extracts
T12856 59200-59210 VBN denotes precleared
T12857 59162-59171 VBN denotes subjected
T12858 59172-59174 IN denotes to
T12859 59175-59194 NN denotes immunoprecipitation
T12860 59195-59199 VBD denotes were
T12861 59211-59215 IN denotes with
T12862 59216-59223 NN denotes Protein
T12863 59224-59225 NN denotes G
T12864 59226-59235 NN denotes Sepharose
T12865 59236-59237 -LRB- denotes (
T12866 59237-59245 NNP denotes Amersham
T12867 59245-59247 , denotes ,
T12868 59247-59257 NNP denotes Piscataway
T12869 59257-59259 , denotes ,
T12870 59259-59262 NNP denotes New
T12871 59263-59267 NNP denotes York
T12872 59267-59269 , denotes ,
T12873 59269-59275 NNP denotes United
T12874 59276-59282 NNP denotes States
T12875 59282-59283 -RRB- denotes )
T12876 59284-59287 CC denotes and
T12877 59288-59297 VBN denotes incubated
T12878 59298-59302 IN denotes with
T12879 59303-59311 NN denotes antibody
T12880 59312-59316 IN denotes with
T12881 59317-59324 NN denotes rocking
T12882 59325-59334 RB denotes overnight
T12883 59335-59337 IN denotes at
T12884 59338-59339 CD denotes 4
T12885 59340-59342 NN denotes °C
T12886 59342-59343 . denotes .
T12887 59343-59430 sentence denotes Protein G Sepharose was added and samples were incubated for 1 h at 4 °C with rocking.
T12888 59344-59351 NN denotes Protein
T12889 59352-59353 NN denotes G
T12890 59354-59363 NN denotes Sepharose
T12891 59368-59373 VBN denotes added
T12892 59364-59367 VBD denotes was
T12893 59374-59377 CC denotes and
T12894 59378-59385 NNS denotes samples
T12895 59391-59400 VBN denotes incubated
T12896 59386-59390 VBD denotes were
T12897 59401-59404 IN denotes for
T12898 59405-59406 CD denotes 1
T12899 59407-59408 NN denotes h
T12900 59409-59411 IN denotes at
T12901 59412-59413 CD denotes 4
T12902 59414-59416 NN denotes °C
T12903 59417-59421 IN denotes with
T12904 59422-59429 NN denotes rocking
T12905 59429-59430 . denotes .
T12906 59430-59603 sentence denotes Samples were washed three times for 5 min each in lysis buffer, and the Protein G Sepharose-antibody-antigen pellet was resuspended in Laemmli buffer and boiled for 10 min.
T12907 59431-59438 NNS denotes Samples
T12908 59444-59450 VBN denotes washed
T12909 59439-59443 VBD denotes were
T12910 59451-59456 CD denotes three
T12911 59457-59462 NNS denotes times
T12912 59463-59466 IN denotes for
T12913 59467-59468 CD denotes 5
T12914 59469-59472 NN denotes min
T12915 59473-59477 RB denotes each
T12916 59478-59480 IN denotes in
T12917 59481-59486 NN denotes lysis
T12918 59487-59493 NN denotes buffer
T12919 59493-59495 , denotes ,
T12920 59495-59498 CC denotes and
T12921 59499-59502 DT denotes the
T12922 59540-59546 NN denotes pellet
T12923 59503-59510 NN denotes Protein
T12924 59511-59512 NN denotes G
T12925 59532-59539 NN denotes antigen
T12926 59513-59522 NN denotes Sepharose
T12927 59522-59523 HYPH denotes -
T12928 59523-59531 NN denotes antibody
T12929 59531-59532 HYPH denotes -
T12930 59551-59562 VBN denotes resuspended
T12931 59547-59550 VBD denotes was
T12932 59563-59565 IN denotes in
T12933 59566-59573 NNP denotes Laemmli
T12934 59574-59580 NN denotes buffer
T12935 59581-59584 CC denotes and
T12936 59585-59591 VBN denotes boiled
T12937 59592-59595 IN denotes for
T12938 59596-59598 CD denotes 10
T12939 59599-59602 NN denotes min
T12940 59602-59603 . denotes .
T12941 59603-59749 sentence denotes Samples were run on SDS-PAGE and transferred to nitrocellulose membrane (Schleicher and Schuell Bioscience, Keene, New Hampshire, United States).
T12942 59604-59611 NNS denotes Samples
T12943 59617-59620 VBN denotes run
T12944 59612-59616 VBD denotes were
T12945 59621-59623 IN denotes on
T12946 59624-59627 NN denotes SDS
T12947 59628-59632 NN denotes PAGE
T12948 59627-59628 HYPH denotes -
T12949 59633-59636 CC denotes and
T12950 59637-59648 VBN denotes transferred
T12951 59649-59651 IN denotes to
T12952 59652-59666 NN denotes nitrocellulose
T12953 59667-59675 NN denotes membrane
T12954 59676-59677 -LRB- denotes (
T12955 59700-59710 NNP denotes Bioscience
T12956 59677-59687 NNP denotes Schleicher
T12957 59688-59691 CC denotes and
T12958 59692-59699 NNP denotes Schuell
T12959 59710-59712 , denotes ,
T12960 59712-59717 NNP denotes Keene
T12961 59717-59719 , denotes ,
T12962 59719-59722 NNP denotes New
T12963 59723-59732 NNP denotes Hampshire
T12964 59732-59734 , denotes ,
T12965 59734-59740 NNP denotes United
T12966 59741-59747 NNP denotes States
T12967 59747-59748 -RRB- denotes )
T12968 59748-59749 . denotes .
T12969 59749-59840 sentence denotes Western blot signals were developed using the enhanced chemiluminescence kit from Amersham
T12970 59750-59757 NNP denotes Western
T12971 59758-59762 NN denotes blot
T12972 59763-59770 NNS denotes signals
T12973 59776-59785 VBN denotes developed
T12974 59771-59775 VBD denotes were
T12975 59786-59791 VBG denotes using
T12976 59792-59795 DT denotes the
T12977 59823-59826 NN denotes kit
T12978 59796-59804 VBN denotes enhanced
T12979 59805-59822 NN denotes chemiluminescence
T12980 59827-59831 IN denotes from
T12981 59832-59840 NNP denotes Amersham
T13041 59842-59846 NN denotes Cell
T13042 59847-59854 NN denotes culture
T13043 59854-59940 sentence denotes Primary keratinocytes were culture in low-calcium medium as previously described [4].
T13044 59855-59862 JJ denotes Primary
T13045 59863-59876 NNS denotes keratinocytes
T13046 59882-59889 VBN denotes culture
T13047 59877-59881 VBD denotes were
T13048 59890-59892 IN denotes in
T13049 59893-59896 JJ denotes low
T13050 59897-59904 NN denotes calcium
T13051 59896-59897 HYPH denotes -
T13052 59905-59911 NN denotes medium
T13053 59912-59914 IN denotes as
T13054 59926-59935 VBN denotes described
T13055 59915-59925 RB denotes previously
T13056 59936-59937 -LRB- denotes [
T13057 59937-59938 CD denotes 4
T13058 59938-59939 -RRB- denotes ]
T13059 59939-59940 . denotes .
T13060 59940-60090 sentence denotes Transient transfections were carried out with FuGENE6 reagent (Roche, Indianapolis, Indiana, United States) according to the manufacturer's protocol.
T13061 59941-59950 JJ denotes Transient
T13062 59951-59964 NNS denotes transfections
T13063 59970-59977 VBN denotes carried
T13064 59965-59969 VBD denotes were
T13065 59978-59981 RP denotes out
T13066 59982-59986 IN denotes with
T13067 59987-59994 NN denotes FuGENE6
T13068 59995-60002 NN denotes reagent
T13069 60003-60004 -LRB- denotes (
T13070 60004-60009 NNP denotes Roche
T13071 60009-60011 , denotes ,
T13072 60011-60023 NNP denotes Indianapolis
T13073 60023-60025 , denotes ,
T13074 60025-60032 NNP denotes Indiana
T13075 60032-60034 , denotes ,
T13076 60034-60040 NNP denotes United
T13077 60041-60047 NNP denotes States
T13078 60047-60048 -RRB- denotes )
T13079 60049-60058 VBG denotes according
T13080 60059-60061 IN denotes to
T13081 60062-60065 DT denotes the
T13082 60066-60078 NN denotes manufacturer
T13083 60081-60089 NN denotes protocol
T13084 60078-60080 POS denotes 's
T13085 60089-60090 . denotes .
T13086 60090-60352 sentence denotes Measurement of β-galactosidase or luciferase levels in promoter activity studies were carried out with the Galacto-Lite assay kit (TROPIX, Bedford, Massachusetts, United States) and the Dual luciferase (Promega, Madison, Wisconsin, United States), respectively.
T13087 60091-60102 NN denotes Measurement
T13088 60177-60184 VBN denotes carried
T13089 60103-60105 IN denotes of
T13090 60106-60107 NN denotes β
T13091 60108-60121 NN denotes galactosidase
T13092 60107-60108 HYPH denotes -
T13093 60136-60142 NNS denotes levels
T13094 60122-60124 CC denotes or
T13095 60125-60135 NN denotes luciferase
T13096 60143-60145 IN denotes in
T13097 60146-60154 NN denotes promoter
T13098 60155-60163 NN denotes activity
T13099 60164-60171 NNS denotes studies
T13100 60172-60176 VBD denotes were
T13101 60185-60188 RP denotes out
T13102 60189-60193 IN denotes with
T13103 60194-60197 DT denotes the
T13104 60217-60220 NN denotes kit
T13105 60198-60205 NNP denotes Galacto
T13106 60206-60210 NNP denotes Lite
T13107 60205-60206 HYPH denotes -
T13108 60211-60216 NN denotes assay
T13109 60221-60222 -LRB- denotes (
T13110 60222-60228 NNP denotes TROPIX
T13111 60228-60230 , denotes ,
T13112 60230-60237 NNP denotes Bedford
T13113 60237-60239 , denotes ,
T13114 60239-60252 NNP denotes Massachusetts
T13115 60252-60254 , denotes ,
T13116 60254-60260 NNP denotes United
T13117 60261-60267 NNP denotes States
T13118 60267-60268 -RRB- denotes )
T13119 60269-60272 CC denotes and
T13120 60273-60276 DT denotes the
T13121 60282-60292 NN denotes luciferase
T13122 60277-60281 JJ denotes Dual
T13123 60293-60294 -LRB- denotes (
T13124 60294-60301 NNP denotes Promega
T13125 60301-60303 , denotes ,
T13126 60303-60310 NNP denotes Madison
T13127 60310-60312 , denotes ,
T13128 60312-60321 NNP denotes Wisconsin
T13129 60321-60323 , denotes ,
T13130 60323-60329 NNP denotes United
T13131 60330-60336 NNP denotes States
T13132 60336-60337 -RRB- denotes )
T13133 60337-60339 , denotes ,
T13134 60339-60351 RB denotes respectively
T13135 60351-60352 . denotes .
T13136 60352-60440 sentence denotes Runella luciferase was cotransfected into cells to correct for transfection efficiency.
T13137 60353-60360 NN denotes Runella
T13138 60361-60371 NN denotes luciferase
T13139 60376-60389 VBN denotes cotransfected
T13140 60372-60375 VBD denotes was
T13141 60390-60394 IN denotes into
T13142 60395-60400 NNS denotes cells
T13143 60401-60403 TO denotes to
T13144 60404-60411 VB denotes correct
T13145 60412-60415 IN denotes for
T13146 60416-60428 NN denotes transfection
T13147 60429-60439 NN denotes efficiency
T13148 60439-60440 . denotes .
T13149 60440-60511 sentence denotes Experiments were done in triplicate and repeated at least three times.
T13150 60441-60452 NNS denotes Experiments
T13151 60458-60462 VBN denotes done
T13152 60453-60457 VBD denotes were
T13153 60463-60465 IN denotes in
T13154 60466-60476 NN denotes triplicate
T13155 60477-60480 CC denotes and
T13156 60481-60489 VBN denotes repeated
T13157 60490-60492 RB denotes at
T13158 60499-60504 CD denotes three
T13159 60493-60498 RBS denotes least
T13160 60505-60510 NNS denotes times
T13161 60510-60511 . denotes .
T13162 60511-60606 sentence denotes Measurements were done on a luminometer (MGM Instruments, Hamden, Connecticut, United States).
T13163 60512-60524 NNS denotes Measurements
T13164 60530-60534 VBN denotes done
T13165 60525-60529 VBD denotes were
T13166 60535-60537 IN denotes on
T13167 60538-60539 DT denotes a
T13168 60540-60551 NN denotes luminometer
T13169 60552-60553 -LRB- denotes (
T13170 60557-60568 NNP denotes Instruments
T13171 60553-60556 NNP denotes MGM
T13172 60568-60570 , denotes ,
T13173 60570-60576 NNP denotes Hamden
T13174 60576-60578 , denotes ,
T13175 60578-60589 NNP denotes Connecticut
T13176 60589-60591 , denotes ,
T13177 60591-60597 NNP denotes United
T13178 60598-60604 NNP denotes States
T13179 60604-60605 -RRB- denotes )
T13180 60605-60606 . denotes .
T13181 60606-60766 sentence denotes For experiments measuring phosphorylation of MAPK, keratinocytes were serum starved for 3 h prior to harvesting of cells by incubation in medium lacking serum.
T13182 60607-60610 IN denotes For
T13183 60683-60690 VBN denotes starved
T13184 60611-60622 NNS denotes experiments
T13185 60623-60632 VBG denotes measuring
T13186 60633-60648 NN denotes phosphorylation
T13187 60649-60651 IN denotes of
T13188 60652-60656 NN denotes MAPK
T13189 60656-60658 , denotes ,
T13190 60658-60671 NNS denotes keratinocytes
T13191 60672-60676 VBD denotes were
T13192 60677-60682 NN denotes serum
T13193 60691-60694 IN denotes for
T13194 60695-60696 CD denotes 3
T13195 60697-60698 NN denotes h
T13196 60699-60704 JJ denotes prior
T13197 60705-60707 IN denotes to
T13198 60708-60718 NN denotes harvesting
T13199 60719-60721 IN denotes of
T13200 60722-60727 NNS denotes cells
T13201 60728-60730 IN denotes by
T13202 60731-60741 NN denotes incubation
T13203 60742-60744 IN denotes in
T13204 60745-60751 NN denotes medium
T13205 60752-60759 VBG denotes lacking
T13206 60760-60765 NN denotes serum
T13207 60765-60766 . denotes .
T13208 60766-60855 sentence denotes Treatment of cells with Wnt- and noggin-conditioned medium was previously described [4].
T13209 60767-60776 NN denotes Treatment
T13210 60841-60850 VBN denotes described
T13211 60777-60779 IN denotes of
T13212 60780-60785 NNS denotes cells
T13213 60786-60790 IN denotes with
T13214 60791-60794 NN denotes Wnt
T13215 60807-60818 VBN denotes conditioned
T13216 60794-60795 HYPH denotes -
T13217 60796-60799 CC denotes and
T13218 60800-60806 NN denotes noggin
T13219 60806-60807 HYPH denotes -
T13220 60819-60825 NN denotes medium
T13221 60826-60829 VBD denotes was
T13222 60830-60840 RB denotes previously
T13223 60851-60852 -LRB- denotes [
T13224 60852-60853 CD denotes 4
T13225 60853-60854 -RRB- denotes ]
T13226 60854-60855 . denotes .
T13308 60857-60867 NNS denotes Constructs
T13309 60867-61148 sentence denotes The 2.2-kb murine Snail promoter was generated by PCR using a forward primer with an XbaI linker sequence, 5′- TCTAGAATTGTTTGCTGCTGTATGGTCTTC-3′, along with a reverse primer with a BglII linker sequence, 5′- AGATCTGTTGGCCAGAGCGACCTAG- GTAG-3′, and mouse genomic DNA as a template.
T13310 60868-60871 DT denotes The
T13311 60892-60900 NN denotes promoter
T13312 60872-60875 CD denotes 2.2
T13313 60876-60878 NN denotes kb
T13314 60875-60876 HYPH denotes -
T13315 60879-60885 JJ denotes murine
T13316 60886-60891 NN denotes Snail
T13317 60905-60914 VBN denotes generated
T13318 60901-60904 VBD denotes was
T13319 60915-60917 IN denotes by
T13320 60918-60921 NN denotes PCR
T13321 60922-60927 VBG denotes using
T13322 60928-60929 DT denotes a
T13323 60938-60944 NN denotes primer
T13324 60930-60937 JJ denotes forward
T13325 60945-60949 IN denotes with
T13326 60950-60952 DT denotes an
T13327 60965-60973 NN denotes sequence
T13328 60953-60957 NN denotes XbaI
T13329 60958-60964 NN denotes linker
T13330 60973-60975 , denotes ,
T13331 60975-60976 CD denotes 5
T13332 60979-61009 NN denotes TCTAGAATTGTTTGCTGCTGTATGGTCTTC
T13333 60976-60977 SYM denotes ′
T13334 60977-60978 HYPH denotes -
T13335 61009-61010 HYPH denotes -
T13336 61010-61011 CD denotes 3
T13337 61011-61012 SYM denotes ′
T13338 61012-61014 , denotes ,
T13339 61014-61019 IN denotes along
T13340 61020-61024 IN denotes with
T13341 61025-61026 DT denotes a
T13342 61035-61041 NN denotes primer
T13343 61027-61034 JJ denotes reverse
T13344 61042-61046 IN denotes with
T13345 61047-61048 DT denotes a
T13346 61062-61070 NN denotes sequence
T13347 61049-61054 NN denotes BglII
T13348 61055-61061 NN denotes linker
T13349 61070-61072 , denotes ,
T13350 61072-61073 CD denotes 5
T13351 61103-61107 NN denotes GTAG
T13352 61073-61074 SYM denotes ′
T13353 61074-61075 HYPH denotes -
T13354 61076-61101 NN denotes AGATCTGTTGGCCAGAGCGACCTAG
T13355 61101-61102 HYPH denotes -
T13356 61107-61108 HYPH denotes -
T13357 61108-61109 CD denotes 3
T13358 61109-61110 SYM denotes ′
T13359 61110-61112 , denotes ,
T13360 61112-61115 CC denotes and
T13361 61116-61121 NN denotes mouse
T13362 61130-61133 NN denotes DNA
T13363 61122-61129 JJ denotes genomic
T13364 61134-61136 IN denotes as
T13365 61137-61138 DT denotes a
T13366 61139-61147 NN denotes template
T13367 61147-61148 . denotes .
T13368 61148-61340 sentence denotes The PCR product was purified with the Gel Extraction Kit (Qiagen, Valencia, California, United States) and ligated into pCRII-TOPO TA vector (Invitrogen, Carlsbad, California, United States).
T13369 61149-61152 DT denotes The
T13370 61157-61164 NN denotes product
T13371 61153-61156 NN denotes PCR
T13372 61169-61177 VBN denotes purified
T13373 61165-61168 VBD denotes was
T13374 61178-61182 IN denotes with
T13375 61183-61186 DT denotes the
T13376 61202-61205 NN denotes Kit
T13377 61187-61190 NN denotes Gel
T13378 61191-61201 NN denotes Extraction
T13379 61206-61207 -LRB- denotes (
T13380 61207-61213 NNP denotes Qiagen
T13381 61213-61215 , denotes ,
T13382 61215-61223 NNP denotes Valencia
T13383 61223-61225 , denotes ,
T13384 61225-61235 NNP denotes California
T13385 61235-61237 , denotes ,
T13386 61237-61243 NNP denotes United
T13387 61244-61250 NNP denotes States
T13388 61250-61251 -RRB- denotes )
T13389 61252-61255 CC denotes and
T13390 61256-61263 VBN denotes ligated
T13391 61264-61268 IN denotes into
T13392 61269-61274 NN denotes pCRII
T13393 61275-61279 NN denotes TOPO
T13394 61274-61275 HYPH denotes -
T13395 61283-61289 NN denotes vector
T13396 61280-61282 NN denotes TA
T13397 61290-61291 -LRB- denotes (
T13398 61291-61301 NNP denotes Invitrogen
T13399 61301-61303 , denotes ,
T13400 61303-61311 NNP denotes Carlsbad
T13401 61311-61313 , denotes ,
T13402 61313-61323 NNP denotes California
T13403 61323-61325 , denotes ,
T13404 61325-61331 NNP denotes United
T13405 61332-61338 NNP denotes States
T13406 61338-61339 -RRB- denotes )
T13407 61339-61340 . denotes .
T13408 61340-61521 sentence denotes The promoter was verified by sequencing and digested with XbaI and BglII and subcloned into the pβ-gal BASIC vector (BD Biosciences Clontech, Palo Alto, California, United States).
T13409 61341-61344 DT denotes The
T13410 61345-61353 NN denotes promoter
T13411 61358-61366 VBN denotes verified
T13412 61354-61357 VBD denotes was
T13413 61367-61369 IN denotes by
T13414 61370-61380 NN denotes sequencing
T13415 61381-61384 CC denotes and
T13416 61385-61393 VBN denotes digested
T13417 61394-61398 IN denotes with
T13418 61399-61403 NN denotes XbaI
T13419 61404-61407 CC denotes and
T13420 61408-61413 NN denotes BglII
T13421 61414-61417 CC denotes and
T13422 61418-61427 VBN denotes subcloned
T13423 61428-61432 IN denotes into
T13424 61433-61436 DT denotes the
T13425 61450-61456 NN denotes vector
T13426 61437-61439 NN denotes pβ
T13427 61440-61443 NN denotes gal
T13428 61439-61440 HYPH denotes -
T13429 61444-61449 NN denotes BASIC
T13430 61457-61458 -LRB- denotes (
T13431 61473-61481 NNP denotes Clontech
T13432 61458-61460 NNP denotes BD
T13433 61461-61472 NNP denotes Biosciences
T13434 61481-61483 , denotes ,
T13435 61483-61487 NNP denotes Palo
T13436 61488-61492 NNP denotes Alto
T13437 61492-61494 , denotes ,
T13438 61494-61504 NNP denotes California
T13439 61504-61506 , denotes ,
T13440 61506-61512 NNP denotes United
T13441 61513-61519 NNP denotes States
T13442 61519-61520 -RRB- denotes )
T13443 61520-61521 . denotes .
T13444 61521-61815 sentence denotes The point mutations in the SMAD binding element was generated with the Quik-Change Kit (Stratagene, La Jolla, California, United States) using the forward primer 5′- GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC-3′ and the reverse primer 5′- GCTTTT- CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC-3′.
T13445 61522-61525 DT denotes The
T13446 61532-61541 NNS denotes mutations
T13447 61526-61531 NN denotes point
T13448 61574-61583 VBN denotes generated
T13449 61542-61544 IN denotes in
T13450 61545-61548 DT denotes the
T13451 61562-61569 NN denotes element
T13452 61549-61553 NN denotes SMAD
T13453 61554-61561 NN denotes binding
T13454 61570-61573 VBD denotes was
T13455 61584-61588 IN denotes with
T13456 61589-61592 DT denotes the
T13457 61605-61608 NNP denotes Kit
T13458 61593-61597 NNP denotes Quik
T13459 61598-61604 NNP denotes Change
T13460 61597-61598 HYPH denotes -
T13461 61609-61610 -LRB- denotes (
T13462 61610-61620 NNP denotes Stratagene
T13463 61620-61622 , denotes ,
T13464 61622-61624 NNP denotes La
T13465 61625-61630 NNP denotes Jolla
T13466 61630-61632 , denotes ,
T13467 61632-61642 NNP denotes California
T13468 61642-61644 , denotes ,
T13469 61644-61650 NNP denotes United
T13470 61651-61657 NNP denotes States
T13471 61657-61658 -RRB- denotes )
T13472 61659-61664 VBG denotes using
T13473 61665-61668 DT denotes the
T13474 61677-61683 NN denotes primer
T13475 61669-61676 JJ denotes forward
T13476 61684-61685 CD denotes 5
T13477 61688-61733 NN denotes GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
T13478 61685-61686 SYM denotes ′
T13479 61686-61687 HYPH denotes -
T13480 61733-61734 HYPH denotes -
T13481 61734-61735 CD denotes 3
T13482 61735-61736 SYM denotes ′
T13483 61737-61740 CC denotes and
T13484 61741-61744 DT denotes the
T13485 61753-61759 NN denotes primer
T13486 61745-61752 JJ denotes reverse
T13487 61760-61761 CD denotes 5
T13488 61772-61811 NN denotes CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
T13489 61761-61762 SYM denotes ′
T13490 61762-61763 HYPH denotes -
T13491 61764-61770 NN denotes GCTTTT
T13492 61770-61771 HYPH denotes -
T13493 61811-61812 HYPH denotes -
T13494 61812-61813 CD denotes 3
T13495 61813-61814 SYM denotes ′
T13496 61814-61815 . denotes .
T13497 61815-62048 sentence denotes The probes for the Snail in situ hybridization were generated against the 3′ UTR by PCR using the forward primer 5′- ACCTTCTCCCGCATGTCCTTGCTCC-3′ and the reverse primer 5′- CTGCTGAGGCATGGTTACAGCTGG-3′, and genomic DNA as a template.
T13498 61816-61819 DT denotes The
T13499 61820-61826 NNS denotes probes
T13500 61868-61877 VBN denotes generated
T13501 61827-61830 IN denotes for
T13502 61831-61834 DT denotes the
T13503 61849-61862 NN denotes hybridization
T13504 61835-61840 NN denotes Snail
T13505 61841-61843 FW denotes in
T13506 61844-61848 FW denotes situ
T13507 61863-61867 VBD denotes were
T13508 61878-61885 IN denotes against
T13509 61886-61889 DT denotes the
T13510 61893-61896 NN denotes UTR
T13511 61890-61891 CD denotes 3
T13512 61891-61892 SYM denotes ′
T13513 61897-61899 IN denotes by
T13514 61900-61903 NN denotes PCR
T13515 61904-61909 VBG denotes using
T13516 61910-61913 DT denotes the
T13517 61922-61928 NN denotes primer
T13518 61914-61921 JJ denotes forward
T13519 61929-61930 CD denotes 5
T13520 61933-61958 NN denotes ACCTTCTCCCGCATGTCCTTGCTCC
T13521 61930-61931 SYM denotes ′
T13522 61931-61932 HYPH denotes -
T13523 61958-61959 HYPH denotes -
T13524 61959-61960 CD denotes 3
T13525 61960-61961 SYM denotes ′
T13526 61962-61965 CC denotes and
T13527 61966-61969 DT denotes the
T13528 61978-61984 NN denotes primer
T13529 61970-61977 JJ denotes reverse
T13530 61985-61986 CD denotes 5
T13531 61989-62013 NN denotes CTGCTGAGGCATGGTTACAGCTGG
T13532 61986-61987 SYM denotes ′
T13533 61987-61988 HYPH denotes -
T13534 62013-62014 HYPH denotes -
T13535 62014-62015 CD denotes 3
T13536 62015-62016 SYM denotes ′
T13537 62016-62018 , denotes ,
T13538 62018-62021 CC denotes and
T13539 62022-62029 JJ denotes genomic
T13540 62030-62033 NN denotes DNA
T13541 62034-62036 IN denotes as
T13542 62037-62038 DT denotes a
T13543 62039-62047 NN denotes template
T13544 62047-62048 . denotes .
T13545 62048-62120 sentence denotes The PCR product was gel purified and ligated into pCRII-TOPO TA vector.
T13546 62049-62052 DT denotes The
T13547 62057-62064 NN denotes product
T13548 62053-62056 NN denotes PCR
T13549 62073-62081 VBN denotes purified
T13550 62065-62068 VBD denotes was
T13551 62069-62072 NN denotes gel
T13552 62082-62085 CC denotes and
T13553 62086-62093 VBN denotes ligated
T13554 62094-62098 IN denotes into
T13555 62099-62104 NN denotes pCRII
T13556 62105-62109 NN denotes TOPO
T13557 62104-62105 HYPH denotes -
T13558 62113-62119 NN denotes vector
T13559 62110-62112 NN denotes TA
T13560 62119-62120 . denotes .
T13561 62120-62281 sentence denotes The pre-LIM domain of Ajuba was generated essentially as described [9], but was fused to GFP by subcloning from the pEGFP-N1 20 vector (BD Biosciences Clontech)
T13562 62121-62124 DT denotes The
T13563 62133-62139 NN denotes domain
T13564 62125-62132 JJ denotes pre-LIM
T13565 62153-62162 VBN denotes generated
T13566 62140-62142 IN denotes of
T13567 62143-62148 NN denotes Ajuba
T13568 62149-62152 VBD denotes was
T13569 62163-62174 RB denotes essentially
T13570 62175-62177 IN denotes as
T13571 62178-62187 VBN denotes described
T13572 62188-62189 -LRB- denotes [
T13573 62189-62190 CD denotes 9
T13574 62190-62191 -RRB- denotes ]
T13575 62191-62193 , denotes ,
T13576 62193-62196 CC denotes but
T13577 62197-62200 VBD denotes was
T13578 62201-62206 VBN denotes fused
T13579 62207-62209 IN denotes to
T13580 62210-62213 NN denotes GFP
T13581 62214-62216 IN denotes by
T13582 62217-62227 VBG denotes subcloning
T13583 62228-62232 IN denotes from
T13584 62233-62236 DT denotes the
T13585 62249-62255 NN denotes vector
T13586 62237-62242 NN denotes pEGFP
T13587 62243-62245 NN denotes N1
T13588 62242-62243 HYPH denotes -
T13589 62246-62248 CD denotes 20
T13590 62256-62257 -LRB- denotes (
T13591 62272-62280 NNP denotes Clontech
T13592 62257-62259 NNP denotes BD
T13593 62260-62271 NNP denotes Biosciences
T13594 62280-62281 -RRB- denotes )
T13649 62283-62285 FW denotes In
T13650 62286-62290 FW denotes situ
T13651 62291-62304 NN denotes hybridization
T13652 62304-62467 sentence denotes The pCRII-TOPO TA vector containing a region of the 3′ UTR of Snail was used as a template to generate digoxigenin-labeled sense and antisense riboprobes (Roche).
T13653 62305-62308 DT denotes The
T13654 62323-62329 NN denotes vector
T13655 62309-62314 NN denotes pCRII
T13656 62315-62319 NN denotes TOPO
T13657 62314-62315 HYPH denotes -
T13658 62320-62322 NN denotes TA
T13659 62377-62381 VBN denotes used
T13660 62330-62340 VBG denotes containing
T13661 62341-62342 DT denotes a
T13662 62343-62349 NN denotes region
T13663 62350-62352 IN denotes of
T13664 62353-62356 DT denotes the
T13665 62360-62363 NN denotes UTR
T13666 62357-62358 CD denotes 3
T13667 62358-62359 SYM denotes ′
T13668 62364-62366 IN denotes of
T13669 62367-62372 NN denotes Snail
T13670 62373-62376 VBD denotes was
T13671 62382-62384 IN denotes as
T13672 62385-62386 DT denotes a
T13673 62387-62395 NN denotes template
T13674 62396-62398 TO denotes to
T13675 62399-62407 VB denotes generate
T13676 62408-62419 NN denotes digoxigenin
T13677 62420-62427 VBN denotes labeled
T13678 62419-62420 HYPH denotes -
T13679 62448-62458 NNS denotes riboprobes
T13680 62428-62433 NN denotes sense
T13681 62434-62437 CC denotes and
T13682 62438-62447 NN denotes antisense
T13683 62459-62460 -LRB- denotes (
T13684 62460-62465 NNP denotes Roche
T13685 62465-62466 -RRB- denotes )
T13686 62466-62467 . denotes .
T13687 62467-62533 sentence denotes The respective probes were obtained by XhoI and BamH1 digestions.
T13688 62468-62471 DT denotes The
T13689 62483-62489 NNS denotes probes
T13690 62472-62482 JJ denotes respective
T13691 62495-62503 VBN denotes obtained
T13692 62490-62494 VBD denotes were
T13693 62504-62506 IN denotes by
T13694 62507-62511 NN denotes XhoI
T13695 62522-62532 NNS denotes digestions
T13696 62512-62515 CC denotes and
T13697 62516-62521 NN denotes BamH1
T13698 62532-62533 . denotes .
T13699 62533-62619 sentence denotes In situ hybridizations were performed on 10-μm thick sections of E17.5 mouse embryos.
T13700 62534-62536 FW denotes In
T13701 62537-62541 FW denotes situ
T13702 62542-62556 NNS denotes hybridizations
T13703 62562-62571 VBN denotes performed
T13704 62557-62561 VBD denotes were
T13705 62572-62574 IN denotes on
T13706 62575-62577 CD denotes 10
T13707 62578-62580 NN denotes μm
T13708 62577-62578 HYPH denotes -
T13709 62581-62586 JJ denotes thick
T13710 62587-62595 NNS denotes sections
T13711 62596-62598 IN denotes of
T13712 62599-62604 NN denotes E17.5
T13713 62611-62618 NNS denotes embryos
T13714 62605-62610 NN denotes mouse
T13715 62618-62619 . denotes .
T13716 62619-62902 sentence denotes The sections were fixed with 4% PFA for 10 min at room temperature, prehybridized at room temperature for 4.5 h, hybridized with the probe (2 μg/ml) at 55 °C for 12–14 h, blocked with 10% NGS, and treated with anti-dig Fab-AP antibody (Roche #1093274) at a 1:2,500 dilution for 3 h.
T13717 62620-62623 DT denotes The
T13718 62624-62632 NNS denotes sections
T13719 62638-62643 VBN denotes fixed
T13720 62633-62637 VBD denotes were
T13721 62644-62648 IN denotes with
T13722 62649-62650 CD denotes 4
T13723 62650-62651 NN denotes %
T13724 62652-62655 NN denotes PFA
T13725 62656-62659 IN denotes for
T13726 62660-62662 CD denotes 10
T13727 62663-62666 NN denotes min
T13728 62667-62669 IN denotes at
T13729 62670-62674 NN denotes room
T13730 62675-62686 NN denotes temperature
T13731 62686-62688 , denotes ,
T13732 62688-62701 VBN denotes prehybridized
T13733 62702-62704 IN denotes at
T13734 62705-62709 NN denotes room
T13735 62710-62721 NN denotes temperature
T13736 62722-62725 IN denotes for
T13737 62726-62729 CD denotes 4.5
T13738 62730-62731 NN denotes h
T13739 62731-62733 , denotes ,
T13740 62733-62743 VBN denotes hybridized
T13741 62744-62748 IN denotes with
T13742 62749-62752 DT denotes the
T13743 62753-62758 NN denotes probe
T13744 62759-62760 -LRB- denotes (
T13745 62762-62764 NN denotes μg
T13746 62760-62761 CD denotes 2
T13747 62764-62765 SYM denotes /
T13748 62765-62767 NN denotes ml
T13749 62767-62768 -RRB- denotes )
T13750 62769-62771 IN denotes at
T13751 62772-62774 CD denotes 55
T13752 62775-62777 NN denotes °C
T13753 62778-62781 IN denotes for
T13754 62782-62784 CD denotes 12
T13755 62785-62787 CD denotes 14
T13756 62784-62785 SYM denotes –
T13757 62788-62789 NN denotes h
T13758 62789-62791 , denotes ,
T13759 62791-62798 VBN denotes blocked
T13760 62799-62803 IN denotes with
T13761 62804-62806 CD denotes 10
T13762 62806-62807 NN denotes %
T13763 62808-62811 NN denotes NGS
T13764 62811-62813 , denotes ,
T13765 62813-62816 CC denotes and
T13766 62817-62824 VBN denotes treated
T13767 62825-62829 IN denotes with
T13768 62830-62838 JJ denotes anti-dig
T13769 62846-62854 NN denotes antibody
T13770 62839-62842 NN denotes Fab
T13771 62843-62845 NN denotes AP
T13772 62842-62843 HYPH denotes -
T13773 62855-62856 -LRB- denotes (
T13774 62856-62861 NNP denotes Roche
T13775 62862-62863 SYM denotes #
T13776 62863-62870 CD denotes 1093274
T13777 62870-62871 -RRB- denotes )
T13778 62872-62874 IN denotes at
T13779 62875-62876 DT denotes a
T13780 62885-62893 NN denotes dilution
T13781 62877-62878 CD denotes 1
T13782 62879-62884 CD denotes 2,500
T13783 62878-62879 SYM denotes :
T13784 62894-62897 IN denotes for
T13785 62898-62899 CD denotes 3
T13786 62900-62901 NN denotes h
T13787 62901-62902 . denotes .
T13788 62902-62984 sentence denotes The sections were incubated with NBT and BCIP until adequate signal was detected.
T13789 62903-62906 DT denotes The
T13790 62907-62915 NNS denotes sections
T13791 62921-62930 VBN denotes incubated
T13792 62916-62920 VBD denotes were
T13793 62931-62935 IN denotes with
T13794 62936-62939 NN denotes NBT
T13795 62940-62943 CC denotes and
T13796 62944-62948 NN denotes BCIP
T13797 62949-62954 IN denotes until
T13798 62975-62983 VBN denotes detected
T13799 62955-62963 JJ denotes adequate
T13800 62964-62970 NN denotes signal
T13801 62971-62974 VBD denotes was
T13802 62983-62984 . denotes .
T13828 62986-63004 NN denotes Immunofluorescence
T13829 63005-63008 CC denotes and
T13830 63009-63029 NN denotes immunohistochemistry
T13831 63029-63127 sentence denotes Tissue samples for immunofluorescence were frozen in OCT and sectioned 10 μm thick on a cryostat.
T13832 63030-63036 NN denotes Tissue
T13833 63037-63044 NNS denotes samples
T13834 63073-63079 VBN denotes frozen
T13835 63045-63048 IN denotes for
T13836 63049-63067 NN denotes immunofluorescence
T13837 63068-63072 VBD denotes were
T13838 63080-63082 IN denotes in
T13839 63083-63086 NN denotes OCT
T13840 63087-63090 CC denotes and
T13841 63091-63100 VBN denotes sectioned
T13842 63101-63103 CD denotes 10
T13843 63104-63106 NN denotes μm
T13844 63107-63112 JJ denotes thick
T13845 63113-63115 IN denotes on
T13846 63116-63117 DT denotes a
T13847 63118-63126 NN denotes cryostat
T13848 63126-63127 . denotes .
T13849 63127-63240 sentence denotes Sections were fixed in 4% paraformaldehyde for 10 min at room temperature, blocked, and stained with antibodies.
T13850 63128-63136 NNS denotes Sections
T13851 63142-63147 VBN denotes fixed
T13852 63137-63141 VBD denotes were
T13853 63148-63150 IN denotes in
T13854 63151-63152 CD denotes 4
T13855 63152-63153 NN denotes %
T13856 63154-63170 NN denotes paraformaldehyde
T13857 63171-63174 IN denotes for
T13858 63175-63177 CD denotes 10
T13859 63178-63181 NN denotes min
T13860 63182-63184 IN denotes at
T13861 63185-63189 NN denotes room
T13862 63190-63201 NN denotes temperature
T13863 63201-63203 , denotes ,
T13864 63203-63210 VBN denotes blocked
T13865 63210-63212 , denotes ,
T13866 63212-63215 CC denotes and
T13867 63216-63223 VBN denotes stained
T13868 63224-63228 IN denotes with
T13869 63229-63239 NNS denotes antibodies
T13870 63239-63240 . denotes .
T13871 63240-63353 sentence denotes Tissue samples for immunohistochemistry were fixed in 4% paraformaldehyde, dehydrated, and embedded in paraffin.
T13872 63241-63247 NN denotes Tissue
T13873 63248-63255 NNS denotes samples
T13874 63286-63291 VBN denotes fixed
T13875 63256-63259 IN denotes for
T13876 63260-63280 NN denotes immunohistochemistry
T13877 63281-63285 VBD denotes were
T13878 63292-63294 IN denotes in
T13879 63295-63296 CD denotes 4
T13880 63296-63297 NN denotes %
T13881 63298-63314 NN denotes paraformaldehyde
T13882 63314-63316 , denotes ,
T13883 63316-63326 VBN denotes dehydrated
T13884 63326-63328 , denotes ,
T13885 63328-63331 CC denotes and
T13886 63332-63340 VBN denotes embedded
T13887 63341-63343 IN denotes in
T13888 63344-63352 NN denotes paraffin
T13889 63352-63353 . denotes .
T13890 63353-63453 sentence denotes Samples were sectioned on a microtome (10 μm thick) and rehydrated prior to staining with antibody.
T13891 63354-63361 NNS denotes Samples
T13892 63367-63376 VBN denotes sectioned
T13893 63362-63366 VBD denotes were
T13894 63377-63379 IN denotes on
T13895 63380-63381 DT denotes a
T13896 63382-63391 NN denotes microtome
T13897 63392-63393 -LRB- denotes (
T13898 63399-63404 JJ denotes thick
T13899 63393-63395 CD denotes 10
T13900 63396-63398 NN denotes μm
T13901 63404-63405 -RRB- denotes )
T13902 63406-63409 CC denotes and
T13903 63410-63420 VBD denotes rehydrated
T13904 63421-63426 IN denotes prior
T13905 63427-63429 IN denotes to
T13906 63430-63438 VBG denotes staining
T13907 63439-63443 IN denotes with
T13908 63444-63452 NN denotes antibody
T13909 63452-63453 . denotes .
T13910 63453-63639 sentence denotes Samples stained with Snail, pMAPK, pSmad2, and cyclin D were antigen unmasked with 10 mM sodium citrate (pH 6) in an Antigen Retriever 2100 (Pickcell Laboratories, Leiden, Netherlands).
T13911 63454-63461 NNS denotes Samples
T13912 63523-63531 VBN denotes unmasked
T13913 63462-63469 VBN denotes stained
T13914 63470-63474 IN denotes with
T13915 63475-63480 NN denotes Snail
T13916 63480-63482 , denotes ,
T13917 63482-63487 NN denotes pMAPK
T13918 63487-63489 , denotes ,
T13919 63489-63495 NN denotes pSmad2
T13920 63495-63497 , denotes ,
T13921 63497-63500 CC denotes and
T13922 63501-63507 NN denotes cyclin
T13923 63508-63509 NN denotes D
T13924 63510-63514 VBD denotes were
T13925 63515-63522 NN denotes antigen
T13926 63532-63536 IN denotes with
T13927 63537-63539 CD denotes 10
T13928 63540-63542 NN denotes mM
T13929 63550-63557 NN denotes citrate
T13930 63543-63549 NN denotes sodium
T13931 63558-63559 -LRB- denotes (
T13932 63559-63561 NN denotes pH
T13933 63562-63563 CD denotes 6
T13934 63563-63564 -RRB- denotes )
T13935 63565-63567 IN denotes in
T13936 63568-63570 DT denotes an
T13937 63579-63588 NNP denotes Retriever
T13938 63571-63578 NNP denotes Antigen
T13939 63589-63593 CD denotes 2100
T13940 63594-63595 -LRB- denotes (
T13941 63604-63616 NNP denotes Laboratories
T13942 63595-63603 NNP denotes Pickcell
T13943 63616-63618 , denotes ,
T13944 63618-63624 NNP denotes Leiden
T13945 63624-63626 , denotes ,
T13946 63626-63637 NNP denotes Netherlands
T13947 63637-63638 -RRB- denotes )
T13948 63638-63639 . denotes .
T13949 63639-63748 sentence denotes The DAB substrate kit (Vector Labs) was used according to manufacturer's instructions to develop the signal.
T13950 63640-63643 DT denotes The
T13951 63658-63661 NN denotes kit
T13952 63644-63647 NN denotes DAB
T13953 63648-63657 NN denotes substrate
T13954 63680-63684 VBN denotes used
T13955 63662-63663 -LRB- denotes (
T13956 63670-63674 NNPS denotes Labs
T13957 63663-63669 NNP denotes Vector
T13958 63674-63675 -RRB- denotes )
T13959 63676-63679 VBD denotes was
T13960 63685-63694 VBG denotes according
T13961 63695-63697 IN denotes to
T13962 63698-63710 NN denotes manufacturer
T13963 63713-63725 NNS denotes instructions
T13964 63710-63712 POS denotes 's
T13965 63726-63728 TO denotes to
T13966 63729-63736 VB denotes develop
T13967 63737-63740 DT denotes the
T13968 63741-63747 NN denotes signal
T13969 63747-63748 . denotes .
T14009 63753-63756 NN denotes PCR
T14010 63752-63753 HYPH denotes -
T14011 63756-63875 sentence denotes RNA was extracted from keratinocytes or skin tissue with Trizol (Invitrogen) according to the manufacturer's protocol.
T14012 63757-63760 NN denotes RNA
T14013 63765-63774 VBN denotes extracted
T14014 63761-63764 VBD denotes was
T14015 63775-63779 IN denotes from
T14016 63780-63793 NNS denotes keratinocytes
T14017 63794-63796 CC denotes or
T14018 63797-63801 NN denotes skin
T14019 63802-63808 NN denotes tissue
T14020 63809-63813 IN denotes with
T14021 63814-63820 NN denotes Trizol
T14022 63821-63822 -LRB- denotes (
T14023 63822-63832 NNP denotes Invitrogen
T14024 63832-63833 -RRB- denotes )
T14025 63834-63843 VBG denotes according
T14026 63844-63846 IN denotes to
T14027 63847-63850 DT denotes the
T14028 63851-63863 NN denotes manufacturer
T14029 63866-63874 NN denotes protocol
T14030 63863-63865 POS denotes 's
T14031 63874-63875 . denotes .
T14032 63875-63958 sentence denotes cDNA was generated using oligo-dT primers and the Superscript II kit (Invitrogen).
T14033 63876-63880 NN denotes cDNA
T14034 63885-63894 VBN denotes generated
T14065 64029-64030 CD denotes 3
T14066 64030-64031 SYM denotes ′
T14067 64031-64032 : denotes ;
T14068 64033-64038 NN denotes Snail
T14069 64039-64046 NN denotes reverse
T14070 64046-64048 : denotes :
T14071 64048-64049 CD denotes 5
T14072 64052-64070 NN denotes GCGAGGGCCTCCGGAGCA
T14073 64049-64050 SYM denotes ′
T14074 64050-64051 HYPH denotes -
T14075 64070-64071 HYPH denotes -
T14076 64071-64072 CD denotes 3
T14077 64072-64073 SYM denotes ′
T14078 64073-64074 : denotes ;
T14079 64075-64080 NN denotes GAPDH
T14080 64081-64088 NN denotes forward
T14081 64089-64090 CD denotes 5
T14082 64093-64117 NN denotes CGTAGACAAAATGGTGAAGGTCGG
T14083 64090-64091 SYM denotes ′
T14084 64091-64092 HYPH denotes -
T14085 64117-64118 HYPH denotes -
T14086 64118-64119 CD denotes 3
T14087 64119-64120 SYM denotes ′
T14088 64120-64121 : denotes ;
T14089 64122-64125 CC denotes and
T14090 64126-64131 NN denotes GAPDH
T14091 64132-64139 NN denotes reverse
T14092 64139-64141 : denotes :
T14093 64141-64142 CD denotes 5
T14094 64145-64167 NN denotes AAGCAGTTGGTGGTGCAGGATG
T14095 64142-64143 SYM denotes ′
T14096 64143-64144 HYPH denotes -
T14097 64167-64168 HYPH denotes -
T14098 64168-64169 CD denotes 3
T14099 64169-64170 SYM denotes ′
T14100 64170-64171 . denotes .
T147 12-19 NN denotes Pathway
T148 0-77 sentence denotes A Signaling Pathway Involving TGF-β2 and Snail in Hair Follicle Morphogenesis
T149 2-11 NN denotes Signaling
T150 20-29 VBG denotes Involving
T151 30-33 NN denotes TGF
T152 34-36 NN denotes β2
T153 33-34 HYPH denotes -
T154 37-40 CC denotes and
T155 41-46 NN denotes Snail
T156 47-49 IN denotes in
T157 50-54 NN denotes Hair
T158 55-63 NN denotes Follicle
T159 64-77 NN denotes Morphogenesis
T160 77-78 sentence denotes
T161 129-288 sentence denotes In a common theme of organogenesis, certain cells within a multipotent epithelial sheet exchange signals with their neighbors and develop into a bud structure.
T162 129-131 IN denotes In
T163 217-225 VB denotes exchange
T164 132-133 DT denotes a
T165 141-146 NN denotes theme
T166 134-140 JJ denotes common
T167 147-149 IN denotes of
T168 150-163 NN denotes organogenesis
T169 163-165 , denotes ,
T170 165-172 JJ denotes certain
T171 173-178 NNS denotes cells
T172 179-185 IN denotes within
T173 186-187 DT denotes a
T174 211-216 NN denotes sheet
T175 188-199 JJ denotes multipotent
T176 200-210 JJ denotes epithelial
T177 226-233 NNS denotes signals
T178 234-238 IN denotes with
T179 239-244 PRP$ denotes their
T180 245-254 NNS denotes neighbors
T181 255-258 CC denotes and
T182 259-266 VBP denotes develop
T183 267-271 IN denotes into
T184 272-273 DT denotes a
T185 278-287 NN denotes structure
T186 274-277 NN denotes bud
T187 287-288 . denotes .
T188 288-544 sentence denotes Using hair bud morphogenesis as a paradigm, we employed mutant mouse models and cultured keratinocytes to dissect the contributions of multiple extracellular cues in orchestrating adhesion dynamics and proliferation to shape the cluster of cells involved.
T189 289-294 VBG denotes Using
T190 336-344 VBD denotes employed
T191 295-299 NN denotes hair
T192 300-303 NN denotes bud
T193 304-317 NN denotes morphogenesis
T194 318-320 IN denotes as
T195 321-322 DT denotes a
T196 323-331 NN denotes paradigm
T197 331-333 , denotes ,
T198 333-335 PRP denotes we
T199 345-351 NN denotes mutant
T200 352-357 NN denotes mouse
T201 358-364 NNS denotes models
T202 365-368 CC denotes and
T203 369-377 VBN denotes cultured
T204 378-391 NNS denotes keratinocytes
T205 392-394 TO denotes to
T206 395-402 VB denotes dissect
T207 403-406 DT denotes the
T208 407-420 NNS denotes contributions
T209 421-423 IN denotes of
T210 424-432 JJ denotes multiple
T211 447-451 NNS denotes cues
T212 433-446 JJ denotes extracellular
T213 452-454 IN denotes in
T214 455-468 VBG denotes orchestrating
T215 469-477 NN denotes adhesion
T216 478-486 NNS denotes dynamics
T217 487-490 CC denotes and
T218 491-504 NN denotes proliferation
T219 505-507 TO denotes to
T220 508-513 VB denotes shape
T221 514-517 DT denotes the
T222 518-525 NN denotes cluster
T223 526-528 IN denotes of
T224 529-534 NNS denotes cells
T225 535-543 VBN denotes involved
T226 543-544 . denotes .
T227 544-745 sentence denotes We found that transforming growth factor β2 signaling is necessary to transiently induce the transcription factor Snail and activate the Ras-mitogen-activated protein kinase (MAPK) pathway in the bud.
T228 545-547 PRP denotes We
T229 548-553 VBD denotes found
T230 554-558 IN denotes that
T231 599-601 VBZ denotes is
T232 559-571 VBG denotes transforming
T233 572-578 NN denotes growth
T234 586-588 NN denotes β2
T235 579-585 NN denotes factor
T236 589-598 NN denotes signaling
T237 602-611 JJ denotes necessary
T238 612-614 TO denotes to
T239 627-633 VB denotes induce
T240 615-626 RB denotes transiently
T241 634-637 DT denotes the
T242 659-664 NN denotes Snail
T243 638-651 NN denotes transcription
T244 652-658 NN denotes factor
T245 665-668 CC denotes and
T246 669-677 VB denotes activate
T247 678-681 DT denotes the
T248 726-733 NN denotes pathway
T249 682-685 NN denotes Ras
T250 712-718 NN denotes kinase
T251 685-686 HYPH denotes -
T252 686-693 NN denotes mitogen
T253 694-703 VBN denotes activated
T254 693-694 HYPH denotes -
T255 704-711 NN denotes protein
T256 719-720 -LRB- denotes (
T257 720-724 NN denotes MAPK
T258 724-725 -RRB- denotes )
T259 734-736 IN denotes in
T260 737-740 DT denotes the
T261 741-744 NN denotes bud
T262 744-745 . denotes .
T263 745-854 sentence denotes In the epidermis, Snail misexpression leads to hyperproliferation and a reduction in intercellular adhesion.
T264 746-748 IN denotes In
T265 784-789 VBZ denotes leads
T266 749-752 DT denotes the
T267 753-762 NN denotes epidermis
T268 762-764 , denotes ,
T269 764-769 NN denotes Snail
T270 770-783 NN denotes misexpression
T271 790-792 IN denotes to
T272 793-811 NN denotes hyperproliferation
T273 812-815 CC denotes and
T274 816-817 DT denotes a
T275 818-827 NN denotes reduction
T276 828-830 IN denotes in
T277 831-844 JJ denotes intercellular
T278 845-853 NN denotes adhesion
T279 853-854 . denotes .
T280 854-1068 sentence denotes When E-cadherin is transcriptionally down-regulated, associated adhesion proteins with dual functions in signaling are released from cell-cell contacts, a process which we demonstrate leads to Ras-MAPK activation.
T281 855-859 WRB denotes When
T282 897-906 VBN denotes regulated
T283 860-861 NN denotes E
T284 862-870 NN denotes cadherin
T285 861-862 HYPH denotes -
T286 871-873 VBZ denotes is
T287 874-891 RB denotes transcriptionally
T288 892-896 RB denotes down
T289 896-897 HYPH denotes -
T290 974-982 VBN denotes released
T291 906-908 , denotes ,
T292 908-918 VBN denotes associated
T293 928-936 NN denotes proteins
T294 919-927 NN denotes adhesion
T295 937-941 IN denotes with
T296 942-946 JJ denotes dual
T297 947-956 NNS denotes functions
T298 957-959 IN denotes in
T299 960-969 NN denotes signaling
T300 970-973 VBP denotes are
T301 983-987 IN denotes from
T302 988-992 NN denotes cell
T303 993-997 NN denotes cell
T304 992-993 HYPH denotes -
T305 998-1006 NNS denotes contacts
T306 1006-1008 , denotes ,
T307 1008-1009 DT denotes a
T308 1010-1017 NN denotes process
T309 1018-1023 WDT denotes which
T310 1027-1038 VBP denotes demonstrate
T311 1024-1026 PRP denotes we
T312 1039-1044 VBZ denotes leads
T313 1045-1047 IN denotes to
T314 1048-1051 NN denotes Ras
T315 1052-1056 NN denotes MAPK
T316 1051-1052 HYPH denotes -
T317 1057-1067 NN denotes activation
T318 1067-1068 . denotes .
T319 1068-1263 sentence denotes These studies provide insights into how multipotent cells within a sheet are stimulated to undergo transcriptional changes that result in proliferation, junctional remodeling, and bud formation.
T320 1069-1074 DT denotes These
T321 1075-1082 NNS denotes studies
T322 1083-1090 VBP denotes provide
T323 1091-1099 NNS denotes insights
T324 1100-1104 IN denotes into
T325 1105-1108 WRB denotes how
T326 1146-1156 VBN denotes stimulated
T327 1109-1120 JJ denotes multipotent
T328 1121-1126 NNS denotes cells
T329 1127-1133 IN denotes within
T330 1134-1135 DT denotes a
T331 1136-1141 NN denotes sheet
T332 1142-1145 VBP denotes are
T333 1157-1159 TO denotes to
T334 1160-1167 VB denotes undergo
T335 1168-1183 JJ denotes transcriptional
T336 1184-1191 NNS denotes changes
T337 1192-1196 WDT denotes that
T338 1197-1203 VBP denotes result
T339 1204-1206 IN denotes in
T340 1207-1220 NN denotes proliferation
T341 1220-1222 , denotes ,
T342 1222-1232 JJ denotes junctional
T343 1233-1243 NN denotes remodeling
T344 1243-1245 , denotes ,
T345 1245-1248 CC denotes and
T346 1249-1252 NN denotes bud
T347 1253-1262 NN denotes formation
T348 1262-1263 . denotes .
T349 1263-1444 sentence denotes This novel signaling pathway further weaves together the web of different morphogens and downstream transcriptional events that guide hair bud formation within the developing skin.
T350 1264-1268 DT denotes This
T351 1285-1292 NN denotes pathway
T352 1269-1274 JJ denotes novel
T353 1275-1284 NN denotes signaling
T354 1301-1307 VBZ denotes weaves
T355 1293-1300 RB denotes further
T356 1308-1316 RP denotes together
T357 1317-1320 DT denotes the
T358 1321-1324 NN denotes web
T359 1325-1327 IN denotes of
T360 1328-1337 JJ denotes different
T361 1338-1348 NNS denotes morphogens
T362 1349-1352 CC denotes and
T363 1353-1363 JJ denotes downstream
T364 1380-1386 NNS denotes events
T365 1364-1379 JJ denotes transcriptional
T366 1387-1391 WDT denotes that
T367 1392-1397 VBP denotes guide
T368 1398-1402 NN denotes hair
T369 1403-1406 NN denotes bud
T370 1407-1416 NN denotes formation
T371 1417-1423 IN denotes within
T372 1424-1427 DT denotes the
T373 1439-1443 NN denotes skin
T374 1428-1438 VBG denotes developing
T375 1443-1444 . denotes .
T1019 1640-1649 JJ denotes Mammalian
T1020 1650-1661 NN denotes development
T1021 1662-1670 VBZ denotes involves
T1022 1671-1674 DT denotes the
T1023 1675-1688 NN denotes morphogenesis
T1024 1689-1691 IN denotes of
T1025 1692-1699 JJ denotes complex
T1026 1718-1728 NNS denotes structures
T1027 1700-1705 CD denotes three
T1028 1706-1717 JJ denotes dimensional
T1029 1705-1706 HYPH denotes -
T1030 1729-1733 IN denotes from
T1031 1734-1743 RB denotes seemingly
T1032 1744-1751 JJ denotes uniform
T1033 1752-1758 NNS denotes sheets
T1034 1759-1761 CC denotes or
T1035 1762-1768 NNS denotes masses
T1036 1769-1771 IN denotes of
T1037 1772-1777 NNS denotes cells
T1038 1777-1778 . denotes .
T1039 1778-1916 sentence denotes A simple bud-like structure initiates the formation of many organs, including lungs, spinal cord, mammary glands, and hair follicles [1].
T1040 1779-1780 DT denotes A
T1041 1797-1806 NN denotes structure
T1042 1781-1787 JJ denotes simple
T1043 1788-1791 NN denotes bud
T1044 1792-1796 JJ denotes like
T1045 1791-1792 HYPH denotes -
T1046 1807-1816 VBZ denotes initiates
T1047 1817-1820 DT denotes the
T1048 1821-1830 NN denotes formation
T1049 1831-1833 IN denotes of
T1050 1834-1838 JJ denotes many
T1051 1839-1845 NNS denotes organs
T1052 1845-1847 , denotes ,
T1053 1847-1856 VBG denotes including
T1054 1857-1862 NNS denotes lungs
T1055 1862-1864 , denotes ,
T1056 1864-1870 JJ denotes spinal
T1057 1871-1875 NN denotes cord
T1058 1875-1877 , denotes ,
T1059 1877-1884 JJ denotes mammary
T1060 1885-1891 NNS denotes glands
T1061 1891-1893 , denotes ,
T1062 1893-1896 CC denotes and
T1063 1897-1901 NN denotes hair
T1064 1902-1911 NNS denotes follicles
T1065 1912-1913 -LRB- denotes [
T1066 1913-1914 CD denotes 1
T1067 1914-1915 -RRB- denotes ]
T1068 1915-1916 . denotes .
T1069 1916-2094 sentence denotes The multipotent, adhering epithelial cells are typically attached to an underlying basal lamina that polarizes the epithelial sheet and separates it from surrounding mesenchyme.
T1070 1917-1920 DT denotes The
T1071 1954-1959 NNS denotes cells
T1072 1921-1932 JJ denotes multipotent
T1073 1932-1934 , denotes ,
T1074 1934-1942 VBG denotes adhering
T1075 1943-1953 JJ denotes epithelial
T1076 1974-1982 VBN denotes attached
T1077 1960-1963 VBP denotes are
T1078 1964-1973 RB denotes typically
T1079 1983-1985 IN denotes to
T1080 1986-1988 DT denotes an
T1081 2006-2012 NN denotes lamina
T1082 1989-1999 VBG denotes underlying
T1083 2000-2005 JJ denotes basal
T1084 2013-2017 WDT denotes that
T1085 2018-2027 VBZ denotes polarizes
T1086 2028-2031 DT denotes the
T1087 2043-2048 NN denotes sheet
T1088 2032-2042 JJ denotes epithelial
T1089 2049-2052 CC denotes and
T1090 2053-2062 VBZ denotes separates
T1091 2063-2065 PRP denotes it
T1092 2066-2070 IN denotes from
T1093 2071-2082 VBG denotes surrounding
T1094 2083-2093 NN denotes mesenchyme
T1095 2093-2094 . denotes .
T1096 2094-2274 sentence denotes Budding morphogenesis is guided by a reciprocal exchange of signals between epithelium and mesenchyme to specify the identity of the organ that will form and to govern its growth.
T1097 2095-2102 VBG denotes Budding
T1098 2103-2116 NN denotes morphogenesis
T1099 2120-2126 VBN denotes guided
T1100 2117-2119 VBZ denotes is
T1101 2127-2129 IN denotes by
T1102 2130-2131 DT denotes a
T1103 2143-2151 NN denotes exchange
T1104 2132-2142 JJ denotes reciprocal
T1105 2152-2154 IN denotes of
T1106 2155-2162 NNS denotes signals
T1107 2163-2170 IN denotes between
T1108 2171-2181 NN denotes epithelium
T1109 2182-2185 CC denotes and
T1110 2186-2196 NN denotes mesenchyme
T1111 2197-2199 TO denotes to
T1112 2200-2207 VB denotes specify
T1113 2208-2211 DT denotes the
T1114 2212-2220 NN denotes identity
T1115 2221-2223 IN denotes of
T1116 2224-2227 DT denotes the
T1117 2228-2233 NN denotes organ
T1118 2234-2238 WDT denotes that
T1119 2244-2248 VB denotes form
T1120 2239-2243 MD denotes will
T1121 2249-2252 CC denotes and
T1122 2253-2255 TO denotes to
T1123 2256-2262 VB denotes govern
T1124 2263-2266 PRP$ denotes its
T1125 2267-2273 NN denotes growth
T1126 2273-2274 . denotes .
T1127 2274-2450 sentence denotes At the helm of these molecular communication pathways are Wnts, bone morphogenic proteins (BMPs), transforming growth factor βs (TGF-βs), and fibroblast growth factors (FGFs).
T1128 2275-2277 IN denotes At
T1129 2329-2332 VBP denotes are
T1130 2278-2281 DT denotes the
T1131 2282-2286 NN denotes helm
T1132 2287-2289 IN denotes of
T1133 2290-2295 DT denotes these
T1134 2320-2328 NNS denotes pathways
T1135 2296-2305 JJ denotes molecular
T1136 2306-2319 NN denotes communication
T1137 2333-2337 NNS denotes Wnts
T1138 2337-2339 , denotes ,
T1139 2339-2343 NN denotes bone
T1140 2356-2364 NN denotes proteins
T1141 2344-2355 JJ denotes morphogenic
T1142 2365-2366 -LRB- denotes (
T1143 2366-2370 NNS denotes BMPs
T1144 2370-2371 -RRB- denotes )
T1145 2371-2373 , denotes ,
T1146 2373-2385 VBG denotes transforming
T1147 2400-2402 NNS denotes βs
T1148 2386-2392 NN denotes growth
T1149 2393-2399 NN denotes factor
T1150 2403-2404 -LRB- denotes (
T1151 2404-2407 NN denotes TGF
T1152 2408-2410 NNS denotes βs
T1153 2407-2408 HYPH denotes -
T1154 2410-2411 -RRB- denotes )
T1155 2411-2413 , denotes ,
T1156 2413-2416 CC denotes and
T1157 2417-2427 NN denotes fibroblast
T1158 2435-2442 NNS denotes factors
T1159 2428-2434 NN denotes growth
T1160 2443-2444 -LRB- denotes (
T1161 2444-2448 NNS denotes FGFs
T1162 2448-2449 -RRB- denotes )
T1163 2449-2450 . denotes .
T1164 2450-2674 sentence denotes Through activation of cell surface transmembrane receptors, these external signaling molecules trigger distinct cascades of intracellular events that culminate in changes in gene expression, growth, and differentiation [2].
T1165 2451-2458 IN denotes Through
T1166 2546-2553 VBP denotes trigger
T1167 2459-2469 NN denotes activation
T1168 2470-2472 IN denotes of
T1169 2473-2477 NN denotes cell
T1170 2478-2485 NN denotes surface
T1171 2500-2509 NNS denotes receptors
T1172 2486-2499 NN denotes transmembrane
T1173 2509-2511 , denotes ,
T1174 2511-2516 DT denotes these
T1175 2536-2545 NNS denotes molecules
T1176 2517-2525 JJ denotes external
T1177 2526-2535 NN denotes signaling
T1178 2554-2562 JJ denotes distinct
T1179 2563-2571 NNS denotes cascades
T1180 2572-2574 IN denotes of
T1181 2575-2588 JJ denotes intracellular
T1182 2589-2595 NNS denotes events
T1183 2596-2600 WDT denotes that
T1184 2601-2610 VBP denotes culminate
T1185 2611-2613 IN denotes in
T1186 2614-2621 NNS denotes changes
T1187 2622-2624 IN denotes in
T1188 2625-2629 NN denotes gene
T1189 2630-2640 NN denotes expression
T1190 2640-2642 , denotes ,
T1191 2642-2648 NN denotes growth
T1192 2648-2650 , denotes ,
T1193 2650-2653 CC denotes and
T1194 2654-2669 NN denotes differentiation
T1195 2670-2671 -LRB- denotes [
T1196 2671-2672 CD denotes 2
T1197 2672-2673 -RRB- denotes ]
T1198 2673-2674 . denotes .
T1199 2674-2847 sentence denotes How this constellation of signals collaborates in tailoring each budding process so that it executes a distinct morphogenetic program has yet to be comprehensively defined.
T1200 2675-2678 WRB denotes How
T1201 2709-2721 VBZ denotes collaborates
T1202 2679-2683 DT denotes this
T1203 2684-2697 NN denotes constellation
T1204 2698-2700 IN denotes of
T1205 2701-2708 NNS denotes signals
T1206 2809-2812 VBZ denotes has
T1207 2722-2724 IN denotes in
T1208 2725-2734 VBG denotes tailoring
T1209 2735-2739 DT denotes each
T1210 2748-2755 NN denotes process
T1211 2740-2747 NN denotes budding
T1212 2756-2758 IN denotes so
T1213 2767-2775 VBZ denotes executes
T1214 2759-2763 IN denotes that
T1215 2764-2766 PRP denotes it
T1216 2776-2777 DT denotes a
T1217 2801-2808 NN denotes program
T1218 2778-2786 JJ denotes distinct
T1219 2787-2800 JJ denotes morphogenetic
T1220 2813-2816 RB denotes yet
T1221 2839-2846 VBN denotes defined
T1222 2817-2819 TO denotes to
T1223 2820-2822 VB denotes be
T1224 2823-2838 RB denotes comprehensively
T1225 2846-2847 . denotes .
T1226 2847-3073 sentence denotes However, the process appears to be patterned at the initial stages of bud formation, since the relative importance of these pathways and their downstream effectors differ as buds begin to develop and cell fates are specified.
T1227 2848-2855 RB denotes However
T1228 2869-2876 VBZ denotes appears
T1229 2855-2857 , denotes ,
T1230 2857-2860 DT denotes the
T1231 2861-2868 NN denotes process
T1232 2877-2879 TO denotes to
T1233 2883-2892 VBN denotes patterned
T1234 2880-2882 VB denotes be
T1235 2893-2895 IN denotes at
T1236 2896-2899 DT denotes the
T1237 2908-2914 NNS denotes stages
T1238 2900-2907 JJ denotes initial
T1239 2915-2917 IN denotes of
T1240 2918-2921 NN denotes bud
T1241 2922-2931 NN denotes formation
T1242 2931-2933 , denotes ,
T1243 2933-2938 IN denotes since
T1244 3012-3018 VBP denotes differ
T1245 2939-2942 DT denotes the
T1246 2952-2962 NN denotes importance
T1247 2943-2951 JJ denotes relative
T1248 2963-2965 IN denotes of
T1249 2966-2971 DT denotes these
T1250 2972-2980 NNS denotes pathways
T1251 2981-2984 CC denotes and
T1252 2985-2990 PRP$ denotes their
T1253 3002-3011 NNS denotes effectors
T1254 2991-3001 JJ denotes downstream
T1255 3019-3021 IN denotes as
T1256 3027-3032 VBP denotes begin
T1257 3022-3026 NNS denotes buds
T1258 3033-3035 TO denotes to
T1259 3036-3043 VB denotes develop
T1260 3044-3047 CC denotes and
T1261 3048-3052 NN denotes cell
T1262 3053-3058 NNS denotes fates
T1263 3063-3072 VBN denotes specified
T1264 3059-3062 VBP denotes are
T1265 3072-3073 . denotes .
T1266 3073-3205 sentence denotes The development of a bud requires a number of coordinated changes in the behavior of the targeted cells within an epithelial sheet.
T1267 3074-3077 DT denotes The
T1268 3078-3089 NN denotes development
T1269 3099-3107 VBZ denotes requires
T1270 3090-3092 IN denotes of
T1271 3093-3094 DT denotes a
T1272 3095-3098 NN denotes bud
T1273 3108-3109 DT denotes a
T1274 3110-3116 NN denotes number
T1275 3117-3119 IN denotes of
T1276 3120-3131 VBN denotes coordinated
T1277 3132-3139 NNS denotes changes
T1278 3140-3142 IN denotes in
T1279 3143-3146 DT denotes the
T1280 3147-3155 NN denotes behavior
T1281 3156-3158 IN denotes of
T1282 3159-3162 DT denotes the
T1283 3172-3177 NNS denotes cells
T1284 3163-3171 VBN denotes targeted
T1285 3178-3184 IN denotes within
T1286 3185-3187 DT denotes an
T1287 3199-3204 NN denotes sheet
T1288 3188-3198 JJ denotes epithelial
T1289 3204-3205 . denotes .
T1290 3205-3389 sentence denotes The process must be accompanied by alterations in the proliferation, polarity, shape, and adhesiveness of selected cells, as well as by modifications in their underlying basal lamina.
T1291 3206-3209 DT denotes The
T1292 3210-3217 NN denotes process
T1293 3226-3237 VBN denotes accompanied
T1294 3218-3222 MD denotes must
T1295 3223-3225 VB denotes be
T1296 3238-3240 IN denotes by
T1297 3241-3252 NNS denotes alterations
T1298 3253-3255 IN denotes in
T1299 3256-3259 DT denotes the
T1300 3260-3273 NN denotes proliferation
T1301 3273-3275 , denotes ,
T1302 3275-3283 NN denotes polarity
T1303 3283-3285 , denotes ,
T1304 3285-3290 NN denotes shape
T1305 3290-3292 , denotes ,
T1306 3292-3295 CC denotes and
T1307 3296-3308 NN denotes adhesiveness
T1308 3309-3311 IN denotes of
T1309 3312-3320 VBN denotes selected
T1310 3321-3326 NNS denotes cells
T1311 3326-3328 , denotes ,
T1312 3328-3330 RB denotes as
T1313 3336-3338 IN denotes as
T1314 3331-3335 RB denotes well
T1315 3339-3341 IN denotes by
T1316 3342-3355 NNS denotes modifications
T1317 3356-3358 IN denotes in
T1318 3359-3364 PRP$ denotes their
T1319 3382-3388 NN denotes lamina
T1320 3365-3375 VBG denotes underlying
T1321 3376-3381 JJ denotes basal
T1322 3388-3389 . denotes .
T1323 3389-3617 sentence denotes Thus, extracellular epithelial-mesenchymal crosstalk must be intricately orchestrated to couple the determination of distinct cell fates with the contemporaneous remodeling of the physical and structural properties of the cell.
T1324 3390-3394 RB denotes Thus
T1325 3463-3475 VBN denotes orchestrated
T1326 3394-3396 , denotes ,
T1327 3396-3409 JJ denotes extracellular
T1328 3433-3442 NN denotes crosstalk
T1329 3410-3420 JJ denotes epithelial
T1330 3421-3432 JJ denotes mesenchymal
T1331 3420-3421 HYPH denotes -
T1332 3443-3447 MD denotes must
T1333 3448-3450 VB denotes be
T1334 3451-3462 RB denotes intricately
T1335 3476-3478 TO denotes to
T1336 3479-3485 VB denotes couple
T1337 3486-3489 DT denotes the
T1338 3490-3503 NN denotes determination
T1339 3504-3506 IN denotes of
T1340 3507-3515 JJ denotes distinct
T1341 3521-3526 NNS denotes fates
T1342 3516-3520 NN denotes cell
T1343 3527-3531 IN denotes with
T1344 3532-3535 DT denotes the
T1345 3552-3562 NN denotes remodeling
T1346 3536-3551 JJ denotes contemporaneous
T1347 3563-3565 IN denotes of
T1348 3566-3569 DT denotes the
T1349 3594-3604 NNS denotes properties
T1350 3570-3578 JJ denotes physical
T1351 3579-3582 CC denotes and
T1352 3583-3593 JJ denotes structural
T1353 3605-3607 IN denotes of
T1354 3608-3611 DT denotes the
T1355 3612-3616 NN denotes cell
T1356 3616-3617 . denotes .
T1357 3617-3733 sentence denotes Among the few dispensable organs, hair follicles offer an excellent model system to study epithelial bud formation.
T1358 3618-3623 IN denotes Among
T1359 3667-3672 VBP denotes offer
T1360 3624-3627 DT denotes the
T1361 3644-3650 NNS denotes organs
T1362 3628-3631 JJ denotes few
T1363 3632-3643 JJ denotes dispensable
T1364 3650-3652 , denotes ,
T1365 3652-3656 NN denotes hair
T1366 3657-3666 NNS denotes follicles
T1367 3673-3675 DT denotes an
T1368 3692-3698 NN denotes system
T1369 3676-3685 JJ denotes excellent
T1370 3686-3691 NN denotes model
T1371 3699-3701 TO denotes to
T1372 3702-3707 VB denotes study
T1373 3708-3718 JJ denotes epithelial
T1374 3719-3722 NN denotes bud
T1375 3723-3732 NN denotes formation
T1376 3732-3733 . denotes .
T1377 3733-3817 sentence denotes Mammalian skin epithelium begins as a single sheet of multipotent ectodermal cells.
T1378 3734-3743 JJ denotes Mammalian
T1379 3749-3759 NN denotes epithelium
T1380 3744-3748 NN denotes skin
T1381 3760-3766 VBZ denotes begins
T1382 3767-3769 IN denotes as
T1383 3770-3771 DT denotes a
T1384 3779-3784 NN denotes sheet
T1385 3772-3778 JJ denotes single
T1386 3785-3787 IN denotes of
T1387 3788-3799 JJ denotes multipotent
T1388 3811-3816 NNS denotes cells
T1389 3800-3810 JJ denotes ectodermal
T1390 3816-3817 . denotes .
T1391 3817-4004 sentence denotes During development, specialized mesenchymal cells populate the skin in a spatially defined pattern to initiate the complex epithelial-mesenchymal crosstalk that will specify the bud [3].
T1392 3818-3824 IN denotes During
T1393 3868-3876 VBP denotes populate
T1394 3825-3836 NN denotes development
T1395 3836-3838 , denotes ,
T1396 3838-3849 JJ denotes specialized
T1397 3862-3867 NNS denotes cells
T1398 3850-3861 JJ denotes mesenchymal
T1399 3877-3880 DT denotes the
T1400 3881-3885 NN denotes skin
T1401 3886-3888 IN denotes in
T1402 3889-3890 DT denotes a
T1403 3909-3916 NN denotes pattern
T1404 3891-3900 RB denotes spatially
T1405 3901-3908 VBN denotes defined
T1406 3917-3919 TO denotes to
T1407 3920-3928 VB denotes initiate
T1408 3929-3932 DT denotes the
T1409 3964-3973 NN denotes crosstalk
T1410 3933-3940 JJ denotes complex
T1411 3941-3951 JJ denotes epithelial
T1412 3952-3963 JJ denotes mesenchymal
T1413 3951-3952 HYPH denotes -
T1414 3974-3978 WDT denotes that
T1415 3984-3991 VB denotes specify
T1416 3979-3983 MD denotes will
T1417 3992-3995 DT denotes the
T1418 3996-3999 NN denotes bud
T1419 4000-4001 -LRB- denotes [
T1420 4001-4002 CD denotes 3
T1421 4002-4003 -RRB- denotes ]
T1422 4003-4004 . denotes .
T1423 4004-4225 sentence denotes Once committed, a small cluster of epithelial cells, the placode, instructs a group of underlying mesenchymal cells to condense and form the nascent dermal papilla, which will be a permanent fixture of the hair follicle.
T1424 4005-4009 IN denotes Once
T1425 4010-4019 VBN denotes committed
T1426 4071-4080 VBZ denotes instructs
T1427 4019-4021 , denotes ,
T1428 4021-4022 DT denotes a
T1429 4029-4036 NN denotes cluster
T1430 4023-4028 JJ denotes small
T1431 4037-4039 IN denotes of
T1432 4040-4050 JJ denotes epithelial
T1433 4051-4056 NNS denotes cells
T1434 4056-4058 , denotes ,
T1435 4058-4061 DT denotes the
T1436 4062-4069 NN denotes placode
T1437 4069-4071 , denotes ,
T1438 4081-4082 DT denotes a
T1439 4083-4088 NN denotes group
T1440 4124-4132 VB denotes condense
T1441 4089-4091 IN denotes of
T1442 4092-4102 VBG denotes underlying
T1443 4115-4120 NNS denotes cells
T1444 4103-4114 JJ denotes mesenchymal
T1445 4121-4123 TO denotes to
T1446 4133-4136 CC denotes and
T1447 4137-4141 VB denotes form
T1448 4142-4145 DT denotes the
T1449 4161-4168 NN denotes papilla
T1450 4146-4153 JJ denotes nascent
T1451 4154-4160 JJ denotes dermal
T1452 4168-4170 , denotes ,
T1453 4170-4175 WDT denotes which
T1454 4181-4183 VB denotes be
T1455 4176-4180 MD denotes will
T1456 4184-4185 DT denotes a
T1457 4196-4203 NN denotes fixture
T1458 4186-4195 JJ denotes permanent
T1459 4204-4206 IN denotes of
T1460 4207-4210 DT denotes the
T1461 4216-4224 NN denotes follicle
T1462 4211-4215 NN denotes hair
T1463 4224-4225 . denotes .
T1464 4225-4462 sentence denotes Subsequent exchanges between the placode and nascent dermal papilla result in further growth of the follicle into the underlying dermis, or down-growth, and eventual differentiation into the six concentric layers of the mature follicle.
T1465 4226-4236 JJ denotes Subsequent
T1466 4237-4246 NNS denotes exchanges
T1467 4294-4300 VBP denotes result
T1468 4247-4254 IN denotes between
T1469 4255-4258 DT denotes the
T1470 4259-4266 NN denotes placode
T1471 4267-4270 CC denotes and
T1472 4271-4278 JJ denotes nascent
T1473 4286-4293 NN denotes papilla
T1474 4279-4285 JJ denotes dermal
T1475 4301-4303 IN denotes in
T1476 4304-4311 JJ denotes further
T1477 4312-4318 NN denotes growth
T1478 4319-4321 IN denotes of
T1479 4322-4325 DT denotes the
T1480 4326-4334 NN denotes follicle
T1481 4335-4339 IN denotes into
T1482 4340-4343 DT denotes the
T1483 4355-4361 NN denotes dermis
T1484 4344-4354 JJ denotes underlying
T1485 4361-4363 , denotes ,
T1486 4363-4365 CC denotes or
T1487 4366-4370 JJ denotes down
T1488 4371-4377 NN denotes growth
T1489 4370-4371 HYPH denotes -
T1490 4377-4379 , denotes ,
T1491 4379-4382 CC denotes and
T1492 4383-4391 JJ denotes eventual
T1493 4392-4407 NN denotes differentiation
T1494 4408-4412 IN denotes into
T1495 4413-4416 DT denotes the
T1496 4432-4438 NNS denotes layers
T1497 4417-4420 CD denotes six
T1498 4421-4431 JJ denotes concentric
T1499 4439-4441 IN denotes of
T1500 4442-4445 DT denotes the
T1501 4453-4461 NN denotes follicle
T1502 4446-4452 JJ denotes mature
T1503 4461-4462 . denotes .
T1504 4462-4732 sentence denotes Previously, we delineated how two respective epithelial and mesenchymal signals, Wnts and the BMP-inhibitory factor noggin, function in concert to induce lymphoid enhancer factor-1/β-catenin (LEF-1/β-catenin)-mediated gene transcription within the follicle placode [4].
T1505 4463-4473 RB denotes Previously
T1506 4478-4488 VBD denotes delineated
T1507 4473-4475 , denotes ,
T1508 4475-4477 PRP denotes we
T1509 4489-4492 WRB denotes how
T1510 4587-4595 VBP denotes function
T1511 4493-4496 CD denotes two
T1512 4535-4542 NNS denotes signals
T1513 4497-4507 JJ denotes respective
T1514 4508-4518 JJ denotes epithelial
T1515 4519-4522 CC denotes and
T1516 4523-4534 JJ denotes mesenchymal
T1517 4542-4544 , denotes ,
T1518 4544-4548 NNS denotes Wnts
T1519 4549-4552 CC denotes and
T1520 4553-4556 DT denotes the
T1521 4572-4578 NN denotes factor
T1522 4557-4560 NN denotes BMP
T1523 4561-4571 JJ denotes inhibitory
T1524 4560-4561 HYPH denotes -
T1525 4579-4585 NN denotes noggin
T1526 4585-4587 , denotes ,
T1527 4596-4598 IN denotes in
T1528 4599-4606 NN denotes concert
T1529 4607-4609 TO denotes to
T1530 4610-4616 VB denotes induce
T1531 4617-4625 JJ denotes lymphoid
T1532 4635-4641 NN denotes factor
T1533 4626-4634 NN denotes enhancer
T1534 4672-4680 VBN denotes mediated
T1535 4641-4642 HYPH denotes -
T1536 4642-4643 CD denotes 1
T1537 4643-4644 HYPH denotes /
T1538 4644-4645 NN denotes β
T1539 4646-4653 NN denotes catenin
T1540 4645-4646 HYPH denotes -
T1541 4654-4655 -LRB- denotes (
T1542 4655-4658 NN denotes LEF
T1543 4658-4659 HYPH denotes -
T1544 4659-4660 CD denotes 1
T1545 4660-4661 HYPH denotes /
T1546 4661-4662 NN denotes β
T1547 4663-4670 NN denotes catenin
T1548 4662-4663 HYPH denotes -
T1549 4670-4671 -RRB- denotes )
T1550 4671-4672 HYPH denotes -
T1551 4686-4699 NN denotes transcription
T1552 4681-4685 NN denotes gene
T1553 4700-4706 IN denotes within
T1554 4707-4710 DT denotes the
T1555 4720-4727 NN denotes placode
T1556 4711-4719 NN denotes follicle
T1557 4728-4729 -LRB- denotes [
T1558 4729-4730 CD denotes 4
T1559 4730-4731 -RRB- denotes ]
T1560 4731-4732 . denotes .
T1561 4732-4997 sentence denotes The downstream changes elicited through convergence of these two early signaling pathways include down-regulation of the gene encoding E-cadherin, the prototypical epithelial cadherin that forms the transmembrane core of intercellular adherens junctions (AJs) [5].
T1562 4733-4736 DT denotes The
T1563 4748-4755 NNS denotes changes
T1564 4737-4747 JJ denotes downstream
T1565 4823-4830 VBP denotes include
T1566 4756-4764 VBN denotes elicited
T1567 4765-4772 IN denotes through
T1568 4773-4784 NN denotes convergence
T1569 4785-4787 IN denotes of
T1570 4788-4793 DT denotes these
T1571 4814-4822 NNS denotes pathways
T1572 4794-4797 CD denotes two
T1573 4798-4803 JJ denotes early
T1574 4804-4813 NN denotes signaling
T1575 4831-4835 JJ denotes down
T1576 4836-4846 NN denotes regulation
T1577 4835-4836 HYPH denotes -
T1578 4847-4849 IN denotes of
T1579 4850-4853 DT denotes the
T1580 4854-4858 NN denotes gene
T1581 4859-4867 VBG denotes encoding
T1582 4868-4869 NN denotes E
T1583 4870-4878 NN denotes cadherin
T1584 4869-4870 HYPH denotes -
T1585 4878-4880 , denotes ,
T1586 4880-4883 DT denotes the
T1587 4908-4916 NN denotes cadherin
T1588 4884-4896 JJ denotes prototypical
T1589 4897-4907 JJ denotes epithelial
T1590 4917-4921 WDT denotes that
T1591 4922-4927 VBZ denotes forms
T1592 4928-4931 DT denotes the
T1593 4946-4950 NN denotes core
T1594 4932-4945 JJ denotes transmembrane
T1595 4951-4953 IN denotes of
T1596 4954-4967 JJ denotes intercellular
T1597 4977-4986 NNS denotes junctions
T1598 4968-4976 NN denotes adherens
T1599 4987-4988 -LRB- denotes (
T1600 4988-4991 NNS denotes AJs
T1601 4991-4992 -RRB- denotes )
T1602 4993-4994 -LRB- denotes [
T1603 4994-4995 CD denotes 5
T1604 4995-4996 -RRB- denotes ]
T1605 4996-4997 . denotes .
T1606 4997-5262 sentence denotes We subsequently showed that when E-cadherin is transgenically elevated in mouse skin, hair follicle morphogenesis is blocked, suggesting that E-cadherin down-regulation is a critical event in governing the adhesion dynamics necessary for budding morphogenesis [4].
T1607 4998-5000 PRP denotes We
T1608 5014-5020 VBD denotes showed
T1609 5001-5013 RB denotes subsequently
T1610 5021-5025 IN denotes that
T1611 5115-5122 VBN denotes blocked
T1612 5026-5030 WRB denotes when
T1613 5060-5068 VBN denotes elevated
T1614 5031-5032 NN denotes E
T1615 5033-5041 NN denotes cadherin
T1616 5032-5033 HYPH denotes -
T1617 5042-5044 VBZ denotes is
T1618 5045-5059 RB denotes transgenically
T1619 5069-5071 IN denotes in
T1620 5072-5077 NN denotes mouse
T1621 5078-5082 NN denotes skin
T1622 5082-5084 , denotes ,
T1623 5084-5088 NN denotes hair
T1624 5089-5097 NN denotes follicle
T1625 5098-5111 NN denotes morphogenesis
T1626 5112-5114 VBZ denotes is
T1627 5122-5124 , denotes ,
T1628 5124-5134 VBG denotes suggesting
T1629 5135-5139 IN denotes that
T1630 5167-5169 VBZ denotes is
T1631 5140-5141 NN denotes E
T1632 5142-5150 NN denotes cadherin
T1633 5141-5142 HYPH denotes -
T1634 5156-5166 NN denotes regulation
T1635 5151-5155 JJ denotes down
T1636 5155-5156 HYPH denotes -
T1637 5170-5171 DT denotes a
T1638 5181-5186 NN denotes event
T1639 5172-5180 JJ denotes critical
T1640 5187-5189 IN denotes in
T1641 5190-5199 VBG denotes governing
T1642 5200-5203 DT denotes the
T1643 5213-5221 NNS denotes dynamics
T1644 5204-5212 NN denotes adhesion
T1645 5222-5231 JJ denotes necessary
T1646 5232-5235 IN denotes for
T1647 5236-5243 NN denotes budding
T1648 5244-5257 NN denotes morphogenesis
T1649 5258-5259 -LRB- denotes [
T1650 5259-5260 CD denotes 4
T1651 5260-5261 -RRB- denotes ]
T1652 5261-5262 . denotes .
T1653 5262-5310 sentence denotes Like LEF-1, E-cadherin also binds to β-catenin.
T1654 5263-5267 IN denotes Like
T1655 5291-5296 VBZ denotes binds
T1656 5268-5271 NN denotes LEF
T1657 5271-5272 HYPH denotes -
T1658 5272-5273 CD denotes 1
T1659 5273-5275 , denotes ,
T1660 5275-5276 NN denotes E
T1661 5277-5285 NN denotes cadherin
T1662 5276-5277 HYPH denotes -
T1663 5286-5290 RB denotes also
T1664 5297-5299 IN denotes to
T1665 5300-5301 NN denotes β
T1666 5302-5309 NN denotes catenin
T1667 5301-5302 HYPH denotes -
T1668 5309-5310 . denotes .
T1669 5310-5573 sentence denotes At sites of cell-cell contact, however, E-cadherin-β-catenin complexes recruit α-catenin, which in turn coordinates the associated actin polymerization dynamics necessary to stabilize nascent AJs and integrate the cytoskeleton across an epithelial sheet [6,7,8].
T1670 5311-5313 IN denotes At
T1671 5382-5389 VBP denotes recruit
T1672 5314-5319 NNS denotes sites
T1673 5320-5322 IN denotes of
T1674 5323-5327 NN denotes cell
T1675 5328-5332 NN denotes cell
T1676 5327-5328 HYPH denotes -
T1677 5333-5340 NN denotes contact
T1678 5340-5342 , denotes ,
T1679 5342-5349 RB denotes however
T1680 5349-5351 , denotes ,
T1681 5351-5352 NN denotes E
T1682 5353-5361 NN denotes cadherin
T1683 5352-5353 HYPH denotes -
T1684 5372-5381 NNS denotes complexes
T1685 5361-5362 HYPH denotes -
T1686 5362-5363 NN denotes β
T1687 5364-5371 NN denotes catenin
T1688 5363-5364 HYPH denotes -
T1689 5390-5391 NN denotes α
T1690 5392-5399 NN denotes catenin
T1691 5391-5392 HYPH denotes -
T1692 5399-5401 , denotes ,
T1693 5401-5406 WDT denotes which
T1694 5415-5426 VBZ denotes coordinates
T1695 5407-5409 IN denotes in
T1696 5410-5414 NN denotes turn
T1697 5427-5430 DT denotes the
T1698 5463-5471 NNS denotes dynamics
T1699 5431-5441 VBN denotes associated
T1700 5442-5447 NN denotes actin
T1701 5448-5462 NN denotes polymerization
T1702 5472-5481 JJ denotes necessary
T1703 5482-5484 TO denotes to
T1704 5485-5494 VB denotes stabilize
T1705 5495-5502 JJ denotes nascent
T1706 5503-5506 NNS denotes AJs
T1707 5507-5510 CC denotes and
T1708 5511-5520 VB denotes integrate
T1709 5521-5524 DT denotes the
T1710 5525-5537 NN denotes cytoskeleton
T1711 5538-5544 IN denotes across
T1712 5545-5547 DT denotes an
T1713 5559-5564 NN denotes sheet
T1714 5548-5558 JJ denotes epithelial
T1715 5565-5566 -LRB- denotes [
T1716 5570-5571 CD denotes 8
T1717 5566-5567 CD denotes 6
T1718 5567-5568 , denotes ,
T1719 5568-5569 CD denotes 7
T1720 5569-5570 , denotes ,
T1721 5571-5572 -RRB- denotes ]
T1722 5572-5573 . denotes .
T1723 5573-5830 sentence denotes α-Catenin also binds to the class III Lin-1, Isl-1, Mec-3 (LIM) protein Ajuba (a member of the zyxin family of proteins), which appears to function dually in both adhesion and in activation of the Ras-mitogen-activated protein kinase (MAPK) pathway [9,10].
T1724 5574-5575 NN denotes α
T1725 5576-5583 NN denotes Catenin
T1726 5575-5576 HYPH denotes -
T1727 5589-5594 VBZ denotes binds
T1728 5584-5588 RB denotes also
T1729 5595-5597 IN denotes to
T1730 5598-5601 DT denotes the
T1731 5638-5645 NN denotes protein
T1732 5602-5607 NN denotes class
T1733 5608-5611 CD denotes III
T1734 5612-5615 NN denotes Lin
T1735 5615-5616 HYPH denotes -
T1736 5616-5617 CD denotes 1
T1737 5617-5619 , denotes ,
T1738 5619-5622 NN denotes Isl
T1739 5622-5623 HYPH denotes -
T1740 5623-5624 CD denotes 1
T1741 5624-5626 , denotes ,
T1742 5626-5629 NN denotes Mec
T1743 5629-5630 HYPH denotes -
T1744 5630-5631 CD denotes 3
T1745 5632-5633 -LRB- denotes (
T1746 5633-5636 NN denotes LIM
T1747 5636-5637 -RRB- denotes )
T1748 5646-5651 NN denotes Ajuba
T1749 5652-5653 -LRB- denotes (
T1750 5653-5654 DT denotes a
T1751 5655-5661 NN denotes member
T1752 5662-5664 IN denotes of
T1753 5665-5668 DT denotes the
T1754 5675-5681 NN denotes family
T1755 5669-5674 NN denotes zyxin
T1756 5682-5684 IN denotes of
T1757 5685-5693 NN denotes proteins
T1758 5693-5694 -RRB- denotes )
T1759 5694-5696 , denotes ,
T1760 5696-5701 WDT denotes which
T1761 5702-5709 VBZ denotes appears
T1762 5710-5712 TO denotes to
T1763 5713-5721 VB denotes function
T1764 5722-5728 RB denotes dually
T1765 5729-5731 IN denotes in
T1766 5732-5736 CC denotes both
T1767 5737-5745 NN denotes adhesion
T1768 5746-5749 CC denotes and
T1769 5750-5752 IN denotes in
T1770 5753-5763 NN denotes activation
T1771 5764-5766 IN denotes of
T1772 5767-5770 DT denotes the
T1773 5815-5822 NN denotes pathway
T1774 5771-5774 NN denotes Ras
T1775 5774-5775 HYPH denotes -
T1776 5775-5782 NN denotes mitogen
T1777 5783-5792 VBN denotes activated
T1778 5782-5783 HYPH denotes -
T1779 5801-5807 NN denotes kinase
T1780 5793-5800 NN denotes protein
T1781 5808-5809 -LRB- denotes (
T1782 5809-5813 NN denotes MAPK
T1783 5813-5814 -RRB- denotes )
T1784 5823-5824 -LRB- denotes [
T1785 5826-5828 CD denotes 10
T1786 5824-5825 CD denotes 9
T1787 5825-5826 , denotes ,
T1788 5828-5829 -RRB- denotes ]
T1789 5829-5830 . denotes .
T1790 5830-5964 sentence denotes Through these links, AJs appear able to couple adhesion with cytoskeletal dynamics as well as with nuclear and cytoplasmic signaling.
T1791 5831-5838 IN denotes Through
T1792 5856-5862 VBP denotes appear
T1793 5839-5844 DT denotes these
T1794 5845-5850 NNS denotes links
T1795 5850-5852 , denotes ,
T1796 5852-5855 NNS denotes AJs
T1797 5863-5867 JJ denotes able
T1798 5868-5870 TO denotes to
T1799 5871-5877 VB denotes couple
T1800 5878-5886 NN denotes adhesion
T1801 5887-5891 IN denotes with
T1802 5892-5904 JJ denotes cytoskeletal
T1803 5905-5913 NNS denotes dynamics
T1804 5914-5916 RB denotes as
T1805 5922-5924 IN denotes as
T1806 5917-5921 RB denotes well
T1807 5925-5929 IN denotes with
T1808 5930-5937 JJ denotes nuclear
T1809 5954-5963 NN denotes signaling
T1810 5938-5941 CC denotes and
T1811 5942-5953 JJ denotes cytoplasmic
T1812 5963-5964 . denotes .
T1813 5964-6112 sentence denotes This provides a framework for conceptualizing why E-cadherin levels appear to impact upon a plethora of developmental processes (reviewed in [11]).
T1814 5965-5969 DT denotes This
T1815 5970-5978 VBZ denotes provides
T1816 5979-5980 DT denotes a
T1817 5981-5990 NN denotes framework
T1818 5991-5994 IN denotes for
T1819 5995-6010 VBG denotes conceptualizing
T1820 6011-6014 WRB denotes why
T1821 6033-6039 VBP denotes appear
T1822 6015-6016 NN denotes E
T1823 6017-6025 NN denotes cadherin
T1824 6016-6017 HYPH denotes -
T1825 6026-6032 NNS denotes levels
T1826 6040-6042 TO denotes to
T1827 6043-6049 VB denotes impact
T1828 6050-6054 IN denotes upon
T1829 6055-6056 DT denotes a
T1830 6057-6065 NN denotes plethora
T1831 6066-6068 IN denotes of
T1832 6069-6082 JJ denotes developmental
T1833 6083-6092 NNS denotes processes
T1834 6093-6094 -LRB- denotes (
T1835 6094-6102 VBN denotes reviewed
T1836 6103-6105 IN denotes in
T1837 6106-6107 -LRB- denotes [
T1838 6107-6109 CD denotes 11
T1839 6109-6110 -RRB- denotes ]
T1840 6110-6111 -RRB- denotes )
T1841 6111-6112 . denotes .
T1842 6112-6396 sentence denotes As we probed more deeply into the underlying mechanisms governing E-cadherin promoter activity, we were intrigued by the close proximity of the LEF-1/β-catenin binding site to a site known to bind the Snail/Slug family of zinc finger transcriptional repressor proteins [12,13,14,15].
T1843 6113-6115 IN denotes As
T1844 6119-6125 VBD denotes probed
T1845 6116-6118 PRP denotes we
T1846 6217-6226 VBN denotes intrigued
T1847 6126-6130 RBR denotes more
T1848 6131-6137 RB denotes deeply
T1849 6138-6142 IN denotes into
T1850 6143-6146 DT denotes the
T1851 6158-6168 NNS denotes mechanisms
T1852 6147-6157 JJ denotes underlying
T1853 6169-6178 VBG denotes governing
T1854 6179-6180 NN denotes E
T1855 6181-6189 NN denotes cadherin
T1856 6180-6181 HYPH denotes -
T1857 6190-6198 NN denotes promoter
T1858 6199-6207 NN denotes activity
T1859 6207-6209 , denotes ,
T1860 6209-6211 PRP denotes we
T1861 6212-6216 VBD denotes were
T1862 6227-6229 IN denotes by
T1863 6230-6233 DT denotes the
T1864 6240-6249 NN denotes proximity
T1865 6234-6239 JJ denotes close
T1866 6250-6252 IN denotes of
T1867 6253-6256 DT denotes the
T1868 6281-6285 NN denotes site
T1869 6257-6260 NN denotes LEF
T1870 6260-6261 HYPH denotes -
T1871 6261-6262 CD denotes 1
T1872 6262-6263 HYPH denotes /
T1873 6263-6264 NN denotes β
T1874 6265-6272 NN denotes catenin
T1875 6264-6265 HYPH denotes -
T1876 6273-6280 NN denotes binding
T1877 6286-6288 IN denotes to
T1878 6289-6290 DT denotes a
T1879 6291-6295 NN denotes site
T1880 6296-6301 VBN denotes known
T1881 6302-6304 TO denotes to
T1882 6305-6309 VB denotes bind
T1883 6310-6313 DT denotes the
T1884 6325-6331 NN denotes family
T1885 6314-6319 NN denotes Snail
T1886 6320-6324 NN denotes Slug
T1887 6319-6320 HYPH denotes /
T1888 6332-6334 IN denotes of
T1889 6335-6339 NN denotes zinc
T1890 6340-6346 NN denotes finger
T1891 6373-6381 NN denotes proteins
T1892 6347-6362 JJ denotes transcriptional
T1893 6363-6372 NN denotes repressor
T1894 6382-6383 -LRB- denotes [
T1895 6392-6394 CD denotes 15
T1896 6383-6385 CD denotes 12
T1897 6385-6386 , denotes ,
T1898 6386-6388 CD denotes 13
T1899 6388-6389 , denotes ,
T1900 6389-6391 CD denotes 14
T1901 6391-6392 , denotes ,
T1902 6394-6395 -RRB- denotes ]
T1903 6395-6396 . denotes .
T1904 6396-6631 sentence denotes Both activity of Snail and down-regulation of E-cadherin play pivotal roles in epithelial to mesenchymal transitions (EMTs), typified by the transformation of polarized, adhering epithelial cells into motile mesenchymal cells [16,17].
T1905 6397-6401 CC denotes Both
T1906 6402-6410 NN denotes activity
T1907 6454-6458 VBP denotes play
T1908 6411-6413 IN denotes of
T1909 6414-6419 NN denotes Snail
T1910 6420-6423 CC denotes and
T1911 6424-6428 NN denotes down
T1912 6429-6439 NN denotes regulation
T1913 6428-6429 HYPH denotes -
T1914 6440-6442 IN denotes of
T1915 6443-6444 NN denotes E
T1916 6445-6453 NN denotes cadherin
T1917 6444-6445 HYPH denotes -
T1918 6459-6466 JJ denotes pivotal
T1919 6467-6472 NNS denotes roles
T1920 6473-6475 IN denotes in
T1921 6476-6486 JJ denotes epithelial
T1922 6502-6513 NNS denotes transitions
T1923 6487-6489 IN denotes to
T1924 6490-6501 JJ denotes mesenchymal
T1925 6514-6515 -LRB- denotes (
T1926 6515-6519 NNS denotes EMTs
T1927 6519-6520 -RRB- denotes )
T1928 6520-6522 , denotes ,
T1929 6522-6530 VBN denotes typified
T1930 6531-6533 IN denotes by
T1931 6534-6537 DT denotes the
T1932 6538-6552 NN denotes transformation
T1933 6553-6555 IN denotes of
T1934 6556-6565 VBN denotes polarized
T1935 6587-6592 NNS denotes cells
T1936 6565-6567 , denotes ,
T1937 6567-6575 VBG denotes adhering
T1938 6576-6586 JJ denotes epithelial
T1939 6593-6597 IN denotes into
T1940 6598-6604 JJ denotes motile
T1941 6617-6622 NNS denotes cells
T1942 6605-6616 JJ denotes mesenchymal
T1943 6623-6624 -LRB- denotes [
T1944 6627-6629 CD denotes 17
T1945 6624-6626 CD denotes 16
T1946 6626-6627 , denotes ,
T1947 6629-6630 -RRB- denotes ]
T1948 6630-6631 . denotes .
T1949 6631-6817 sentence denotes Bud formation differs from an EMT in that E-cadherin activity needs to be down-regulated but not prevented, so that adhesive junctions are remodeled rather than quantitatively impaired.
T1950 6632-6635 NN denotes Bud
T1951 6636-6645 NN denotes formation
T1952 6646-6653 VBZ denotes differs
T1953 6654-6658 IN denotes from
T1954 6659-6661 DT denotes an
T1955 6662-6665 NN denotes EMT
T1956 6666-6668 IN denotes in
T1957 6694-6699 VBZ denotes needs
T1958 6669-6673 DT denotes that
T1959 6674-6675 NN denotes E
T1960 6676-6684 NN denotes cadherin
T1961 6675-6676 HYPH denotes -
T1962 6685-6693 NN denotes activity
T1963 6700-6702 TO denotes to
T1964 6711-6720 VBN denotes regulated
T1965 6703-6705 VB denotes be
T1966 6706-6710 RB denotes down
T1967 6710-6711 HYPH denotes -
T1968 6721-6724 CC denotes but
T1969 6725-6728 RB denotes not
T1970 6729-6738 VBN denotes prevented
T1971 6738-6740 , denotes ,
T1972 6740-6742 IN denotes so
T1973 6771-6780 VBN denotes remodeled
T1974 6743-6747 IN denotes that
T1975 6748-6756 JJ denotes adhesive
T1976 6757-6766 NNS denotes junctions
T1977 6767-6770 VBP denotes are
T1978 6781-6787 RB denotes rather
T1979 6788-6792 IN denotes than
T1980 6793-6807 RB denotes quantitatively
T1981 6808-6816 VBN denotes impaired
T1982 6816-6817 . denotes .
T1983 6817-7035 sentence denotes Supportive of an underlying ability to fine-tune cadherin expression at the transcriptional level, Snail seems to have an additive effect with LEF-1/β-catenin in negatively modulating E-cadherin promoter activity [4].
T1984 6818-6828 JJ denotes Supportive
T1985 6923-6928 VBZ denotes seems
T1986 6829-6831 IN denotes of
T1987 6832-6834 DT denotes an
T1988 6846-6853 NN denotes ability
T1989 6835-6845 JJ denotes underlying
T1990 6854-6856 TO denotes to
T1991 6862-6866 VB denotes tune
T1992 6857-6861 RB denotes fine
T1993 6861-6862 HYPH denotes -
T1994 6867-6875 NN denotes cadherin
T1995 6876-6886 NN denotes expression
T1996 6887-6889 IN denotes at
T1997 6890-6893 DT denotes the
T1998 6910-6915 NN denotes level
T1999 6894-6909 JJ denotes transcriptional
T2000 6915-6917 , denotes ,
T2001 6917-6922 NN denotes Snail
T2002 6929-6931 TO denotes to
T2003 6932-6936 VB denotes have
T2004 6937-6939 DT denotes an
T2005 6949-6955 NN denotes effect
T2006 6940-6948 JJ denotes additive
T2007 6956-6960 IN denotes with
T2008 6961-6964 NN denotes LEF
T2009 6964-6965 HYPH denotes -
T2010 6965-6966 CD denotes 1
T2011 6966-6967 HYPH denotes /
T2012 6967-6968 NN denotes β
T2013 6969-6976 NN denotes catenin
T2014 6968-6969 HYPH denotes -
T2015 6977-6979 IN denotes in
T2016 6980-6990 RB denotes negatively
T2017 6991-7001 VBG denotes modulating
T2018 7002-7003 NN denotes E
T2019 7004-7012 NN denotes cadherin
T2020 7003-7004 HYPH denotes -
T2021 7013-7021 NN denotes promoter
T2022 7022-7030 NN denotes activity
T2023 7031-7032 -LRB- denotes [
T2024 7032-7033 CD denotes 4
T2025 7033-7034 -RRB- denotes ]
T2026 7034-7035 . denotes .
T2027 7035-7220 sentence denotes In the present study, we discovered that Snail is expressed briefly at an early stage of hair bud formation, when E-cadherin down-regulation and activation of proliferation take place.
T2028 7036-7038 IN denotes In
T2029 7061-7071 VBD denotes discovered
T2030 7039-7042 DT denotes the
T2031 7051-7056 NN denotes study
T2032 7043-7050 JJ denotes present
T2033 7056-7058 , denotes ,
T2034 7058-7060 PRP denotes we
T2035 7072-7076 IN denotes that
T2036 7086-7095 VBN denotes expressed
T2037 7077-7082 NN denotes Snail
T2038 7083-7085 VBZ denotes is
T2039 7096-7103 RB denotes briefly
T2040 7104-7106 IN denotes at
T2041 7107-7109 DT denotes an
T2042 7116-7121 NN denotes stage
T2043 7110-7115 JJ denotes early
T2044 7122-7124 IN denotes of
T2045 7125-7129 NN denotes hair
T2046 7130-7133 NN denotes bud
T2047 7134-7143 NN denotes formation
T2048 7143-7145 , denotes ,
T2049 7145-7149 WRB denotes when
T2050 7209-7213 VBP denotes take
T2051 7150-7151 NN denotes E
T2052 7152-7160 NN denotes cadherin
T2053 7151-7152 HYPH denotes -
T2054 7161-7165 JJ denotes down
T2055 7166-7176 NN denotes regulation
T2056 7165-7166 HYPH denotes -
T2057 7177-7180 CC denotes and
T2058 7181-7191 NN denotes activation
T2059 7192-7194 IN denotes of
T2060 7195-7208 NN denotes proliferation
T2061 7214-7219 NN denotes place
T2062 7219-7220 . denotes .
T2063 7220-7323 sentence denotes Thereafter, Snail disappears and remains absent during subsequent follicle down-growth and maturation.
T2064 7221-7231 RB denotes Thereafter
T2065 7239-7249 VBZ denotes disappears
T2066 7231-7233 , denotes ,
T2067 7233-7238 NN denotes Snail
T2068 7250-7253 CC denotes and
T2069 7254-7261 VBZ denotes remains
T2070 7262-7268 JJ denotes absent
T2071 7269-7275 IN denotes during
T2072 7276-7286 JJ denotes subsequent
T2073 7301-7307 NN denotes growth
T2074 7287-7295 NN denotes follicle
T2075 7296-7300 JJ denotes down
T2076 7300-7301 HYPH denotes -
T2077 7308-7311 CC denotes and
T2078 7312-7322 NN denotes maturation
T2079 7322-7323 . denotes .
T2080 7323-7583 sentence denotes This exquisite pattern appears to be functionally relevant since altering it in vivo correspondingly affects features associated with hair bud formation, including down-regulation of E-cadherin, increased proliferation, and repressed terminal differentiation.
T2081 7324-7328 DT denotes This
T2082 7339-7346 NN denotes pattern
T2083 7329-7338 JJ denotes exquisite
T2084 7347-7354 VBZ denotes appears
T2085 7355-7357 TO denotes to
T2086 7358-7360 VB denotes be
T2087 7361-7373 RB denotes functionally
T2088 7374-7382 JJ denotes relevant
T2089 7383-7388 IN denotes since
T2090 7425-7432 VBZ denotes affects
T2091 7389-7397 VBG denotes altering
T2092 7398-7400 PRP denotes it
T2093 7401-7403 FW denotes in
T2094 7404-7408 FW denotes vivo
T2095 7409-7424 RB denotes correspondingly
T2096 7433-7441 NNS denotes features
T2097 7442-7452 VBN denotes associated
T2098 7453-7457 IN denotes with
T2099 7458-7462 NN denotes hair
T2100 7463-7466 NN denotes bud
T2101 7467-7476 NN denotes formation
T2102 7476-7478 , denotes ,
T2103 7478-7487 VBG denotes including
T2104 7488-7492 NN denotes down
T2105 7493-7503 NN denotes regulation
T2106 7492-7493 HYPH denotes -
T2107 7504-7506 IN denotes of
T2108 7507-7508 NN denotes E
T2109 7509-7517 NN denotes cadherin
T2110 7508-7509 HYPH denotes -
T2111 7517-7519 , denotes ,
T2112 7519-7528 VBN denotes increased
T2113 7529-7542 NN denotes proliferation
T2114 7542-7544 , denotes ,
T2115 7544-7547 CC denotes and
T2116 7548-7557 VBN denotes repressed
T2117 7567-7582 NN denotes differentiation
T2118 7558-7566 JJ denotes terminal
T2119 7582-7583 . denotes .
T2120 7583-7829 sentence denotes Although the temporal spike of Snail in the hair bud is reflected at the mRNA level and seems to follow Wnt signaling and BMP inhibition, LEF-1/β-catenin activation does not appear to induce Snail gene expression in embryonic skin keratinocytes.
T2121 7584-7592 IN denotes Although
T2122 7640-7649 VBN denotes reflected
T2123 7593-7596 DT denotes the
T2124 7606-7611 NN denotes spike
T2125 7597-7605 JJ denotes temporal
T2126 7612-7614 IN denotes of
T2127 7615-7620 NN denotes Snail
T2128 7621-7623 IN denotes in
T2129 7624-7627 DT denotes the
T2130 7633-7636 NN denotes bud
T2131 7628-7632 NN denotes hair
T2132 7637-7639 VBZ denotes is
T2133 7758-7764 VB denotes appear
T2134 7650-7652 IN denotes at
T2135 7653-7656 DT denotes the
T2136 7662-7667 NN denotes level
T2137 7657-7661 NN denotes mRNA
T2138 7668-7671 CC denotes and
T2139 7672-7677 VBZ denotes seems
T2140 7678-7680 TO denotes to
T2141 7681-7687 VB denotes follow
T2142 7688-7691 NN denotes Wnt
T2143 7692-7701 NN denotes signaling
T2144 7702-7705 CC denotes and
T2145 7706-7709 NN denotes BMP
T2146 7710-7720 NN denotes inhibition
T2147 7720-7722 , denotes ,
T2148 7722-7725 NN denotes LEF
T2149 7738-7748 NN denotes activation
T2150 7725-7726 HYPH denotes -
T2151 7726-7727 CD denotes 1
T2152 7727-7728 HYPH denotes /
T2153 7728-7729 NN denotes β
T2154 7730-7737 NN denotes catenin
T2155 7729-7730 HYPH denotes -
T2156 7749-7753 VBZ denotes does
T2157 7754-7757 RB denotes not
T2158 7765-7767 TO denotes to
T2159 7768-7774 VB denotes induce
T2160 7775-7780 NN denotes Snail
T2161 7786-7796 NN denotes expression
T2162 7781-7785 NN denotes gene
T2163 7797-7799 IN denotes in
T2164 7800-7809 JJ denotes embryonic
T2165 7815-7828 NNS denotes keratinocytes
T2166 7810-7814 NN denotes skin
T2167 7828-7829 . denotes .
T2168 7829-8088 sentence denotes In contrast, we provide in vitro, transgenic (Tg), and gene targeting evidence to show that TGF-β2 and small phenotype– and mothers against decapentaplegic–related protein 2 (SMAD2) signaling are upstream inducers of Snail gene expression in skin epithelium.
T2169 7830-7832 IN denotes In
T2170 7846-7853 VBP denotes provide
T2171 7833-7841 NN denotes contrast
T2172 7841-7843 , denotes ,
T2173 7843-7845 PRP denotes we
T2174 7854-7856 FW denotes in
T2175 7857-7862 FW denotes vitro
T2176 7900-7908 NN denotes evidence
T2177 7862-7864 , denotes ,
T2178 7864-7874 JJ denotes transgenic
T2179 7875-7876 -LRB- denotes (
T2180 7876-7878 JJ denotes Tg
T2181 7878-7879 -RRB- denotes )
T2182 7879-7881 , denotes ,
T2183 7881-7884 CC denotes and
T2184 7885-7889 NN denotes gene
T2185 7890-7899 VBG denotes targeting
T2186 7909-7911 TO denotes to
T2187 7912-7916 VB denotes show
T2188 7917-7921 IN denotes that
T2189 8022-8025 VBP denotes are
T2190 7922-7925 NN denotes TGF
T2191 7926-7928 NN denotes β2
T2192 7925-7926 HYPH denotes -
T2193 8012-8021 NN denotes signaling
T2194 7929-7932 CC denotes and
T2195 7933-7938 JJ denotes small
T2196 7939-7948 NN denotes phenotype
T2197 7986-7993 VBN denotes related
T2198 7948-7949 -LRB- denotes –
T2199 7950-7953 CC denotes and
T2200 7954-7961 NNS denotes mothers
T2201 7962-7969 IN denotes against
T2202 7970-7985 NN denotes decapentaplegic
T2203 7985-7986 -RRB- denotes –
T2204 7994-8001 NN denotes protein
T2205 8002-8003 CD denotes 2
T2206 8004-8005 -LRB- denotes (
T2207 8005-8010 NN denotes SMAD2
T2208 8010-8011 -RRB- denotes )
T2209 8026-8034 JJ denotes upstream
T2210 8035-8043 NNS denotes inducers
T2211 8044-8046 IN denotes of
T2212 8047-8052 NN denotes Snail
T2213 8058-8068 NN denotes expression
T2214 8053-8057 NN denotes gene
T2215 8069-8071 IN denotes in
T2216 8072-8076 NN denotes skin
T2217 8077-8087 NN denotes epithelium
T2218 8087-8088 . denotes .
T2219 8088-8219 sentence denotes In the absence of TGF-β2 signaling and Snail gene expression, hair placodes can form, but further follicle down-growth is blocked.
T2220 8089-8091 IN denotes In
T2221 8169-8173 VB denotes form
T2222 8092-8095 DT denotes the
T2223 8096-8103 NN denotes absence
T2224 8104-8106 IN denotes of
T2225 8107-8110 NN denotes TGF
T2226 8111-8113 NN denotes β2
T2227 8110-8111 HYPH denotes -
T2228 8114-8123 NN denotes signaling
T2229 8124-8127 CC denotes and
T2230 8128-8133 NN denotes Snail
T2231 8139-8149 NN denotes expression
T2232 8134-8138 NN denotes gene
T2233 8149-8151 , denotes ,
T2234 8151-8155 NN denotes hair
T2235 8156-8164 NNS denotes placodes
T2236 8165-8168 MD denotes can
T2237 8173-8175 , denotes ,
T2238 8175-8178 CC denotes but
T2239 8179-8186 JJ denotes further
T2240 8201-8207 NN denotes growth
T2241 8187-8195 NN denotes follicle
T2242 8196-8200 JJ denotes down
T2243 8200-8201 HYPH denotes -
T2244 8211-8218 VBN denotes blocked
T2245 8208-8210 VBZ denotes is
T2246 8218-8219 . denotes .
T2247 8219-8397 sentence denotes Our studies point to the view that Snail likely functions downstream of cell fate specification, at a stage where the bud begins to exhibit enhanced proliferation and migration.
T2248 8220-8223 PRP$ denotes Our
T2249 8224-8231 NNS denotes studies
T2250 8232-8237 VBP denotes point
T2251 8238-8240 IN denotes to
T2252 8241-8244 DT denotes the
T2253 8245-8249 NN denotes view
T2254 8250-8254 IN denotes that
T2255 8268-8277 VBZ denotes functions
T2256 8255-8260 NN denotes Snail
T2257 8261-8267 RB denotes likely
T2258 8278-8288 RB denotes downstream
T2259 8289-8291 IN denotes of
T2260 8292-8296 NN denotes cell
T2261 8297-8301 NN denotes fate
T2262 8302-8315 NN denotes specification
T2263 8315-8317 , denotes ,
T2264 8317-8319 IN denotes at
T2265 8320-8321 DT denotes a
T2266 8322-8327 NN denotes stage
T2267 8328-8333 WRB denotes where
T2268 8342-8348 VBZ denotes begins
T2269 8334-8337 DT denotes the
T2270 8338-8341 NN denotes bud
T2271 8349-8351 TO denotes to
T2272 8352-8359 VB denotes exhibit
T2273 8360-8368 VBN denotes enhanced
T2274 8369-8382 NN denotes proliferation
T2275 8383-8386 CC denotes and
T2276 8387-8396 NN denotes migration
T2277 8396-8397 . denotes .
T2702 8408-8413 NN denotes Snail
T2703 8414-8418 NN denotes mRNA
T2704 8435-8444 VBN denotes Expressed
T2705 8419-8422 CC denotes and
T2706 8423-8430 NN denotes Protein
T2707 8431-8434 VBP denotes Are
T2708 8445-8456 RB denotes Transiently
T2709 8457-8459 IN denotes at
T2710 8460-8463 DT denotes the
T2711 8473-8478 NN denotes Stage
T2712 8464-8468 NN denotes Hair
T2713 8469-8472 NN denotes Bud
T2714 8479-8481 IN denotes of
T2715 8482-8490 NN denotes Follicle
T2716 8491-8504 NN denotes Morphogenesis
T2717 8504-8721 sentence denotes Although Snail family members are most frequently associated with EMTs, they also participate in many malignant processes involving a down-regulation but not a quantitative abrogation of intercellular junctions [18].
T2718 8505-8513 IN denotes Although
T2719 8555-8565 VBN denotes associated
T2720 8514-8519 NN denotes Snail
T2721 8527-8534 NNS denotes members
T2722 8520-8526 NN denotes family
T2723 8535-8538 VBP denotes are
T2724 8539-8543 RBS denotes most
T2725 8544-8554 RB denotes frequently
T2726 8587-8598 VBP denotes participate
T2727 8566-8570 IN denotes with
T2728 8571-8575 NNS denotes EMTs
T2729 8575-8577 , denotes ,
T2730 8577-8581 PRP denotes they
T2731 8582-8586 RB denotes also
T2732 8599-8601 IN denotes in
T2733 8602-8606 JJ denotes many
T2734 8617-8626 NNS denotes processes
T2735 8607-8616 JJ denotes malignant
T2736 8627-8636 VBG denotes involving
T2737 8637-8638 DT denotes a
T2738 8644-8654 NN denotes regulation
T2739 8639-8643 JJ denotes down
T2740 8643-8644 HYPH denotes -
T2741 8655-8658 CC denotes but
T2742 8659-8662 RB denotes not
T2743 8663-8664 DT denotes a
T2744 8678-8688 NN denotes abrogation
T2745 8665-8677 JJ denotes quantitative
T2746 8689-8691 IN denotes of
T2747 8692-8705 JJ denotes intercellular
T2748 8706-8715 NNS denotes junctions
T2749 8716-8717 -LRB- denotes [
T2750 8717-8719 CD denotes 18
T2751 8719-8720 -RRB- denotes ]
T2752 8720-8721 . denotes .
T2753 8721-8906 sentence denotes The range of developmental processes in which Snail family members have been implicated thus includes the type of epithelial remodeling that is observed in hair follicle bud formation.
T2754 8722-8725 DT denotes The
T2755 8726-8731 NN denotes range
T2756 8815-8823 VBZ denotes includes
T2757 8732-8734 IN denotes of
T2758 8735-8748 JJ denotes developmental
T2759 8749-8758 NNS denotes processes
T2760 8759-8761 IN denotes in
T2761 8799-8809 VBN denotes implicated
T2762 8762-8767 WDT denotes which
T2763 8768-8773 NN denotes Snail
T2764 8781-8788 NNS denotes members
T2765 8774-8780 NN denotes family
T2766 8789-8793 VBP denotes have
T2767 8794-8798 VBN denotes been
T2768 8810-8814 RB denotes thus
T2769 8824-8827 DT denotes the
T2770 8828-8832 NN denotes type
T2771 8833-8835 IN denotes of
T2772 8836-8846 JJ denotes epithelial
T2773 8847-8857 NN denotes remodeling
T2774 8858-8862 WDT denotes that
T2775 8866-8874 VBN denotes observed
T2776 8863-8865 VBZ denotes is
T2777 8875-8877 IN denotes in
T2778 8878-8882 NN denotes hair
T2779 8883-8891 NN denotes follicle
T2780 8892-8895 NN denotes bud
T2781 8896-8905 NN denotes formation
T2782 8905-8906 . denotes .
T2783 8906-9260 sentence denotes Given our prior observation that exogenously added Snail can participate with LEF-1/β-catenin in down-regulating E-cadherin expression in keratinocytes [4], coupled with the established requirement for LEF-1/β-catenin in hair follicle morphogenesis [4,19], we turned to addressing whether Snail/Slug family members might also participate in the process.
T2784 8907-8912 VBN denotes Given
T2785 9167-9173 VBD denotes turned
T2786 8913-8916 PRP$ denotes our
T2787 8923-8934 NN denotes observation
T2788 8917-8922 JJ denotes prior
T2789 8935-8939 IN denotes that
T2790 8968-8979 VB denotes participate
T2791 8940-8951 RB denotes exogenously
T2792 8952-8957 VBN denotes added
T2793 8958-8963 NN denotes Snail
T2794 8964-8967 MD denotes can
T2795 8980-8984 IN denotes with
T2796 8985-8988 NN denotes LEF
T2797 8988-8989 HYPH denotes -
T2798 8989-8990 CD denotes 1
T2799 8990-8991 HYPH denotes /
T2800 8991-8992 NN denotes β
T2801 8993-9000 NN denotes catenin
T2802 8992-8993 HYPH denotes -
T2803 9001-9003 IN denotes in
T2804 9004-9008 RB denotes down
T2805 9009-9019 VBG denotes regulating
T2806 9008-9009 HYPH denotes -
T2807 9020-9021 NN denotes E
T2808 9022-9030 NN denotes cadherin
T2809 9021-9022 HYPH denotes -
T2810 9031-9041 NN denotes expression
T2811 9042-9044 IN denotes in
T2812 9045-9058 NNS denotes keratinocytes
T2813 9059-9060 -LRB- denotes [
T2814 9060-9061 CD denotes 4
T2815 9061-9062 -RRB- denotes ]
T2816 9062-9064 , denotes ,
T2817 9064-9071 VBN denotes coupled
T2818 9072-9076 IN denotes with
T2819 9077-9080 DT denotes the
T2820 9093-9104 NN denotes requirement
T2821 9081-9092 JJ denotes established
T2822 9105-9108 IN denotes for
T2823 9109-9112 NN denotes LEF
T2824 9112-9113 HYPH denotes -
T2825 9113-9114 CD denotes 1
T2826 9114-9115 HYPH denotes /
T2827 9115-9116 NN denotes β
T2828 9117-9124 NN denotes catenin
T2829 9116-9117 HYPH denotes -
T2830 9125-9127 IN denotes in
T2831 9128-9132 NN denotes hair
T2832 9133-9141 NN denotes follicle
T2833 9142-9155 NN denotes morphogenesis
T2834 9156-9157 -LRB- denotes [
T2835 9159-9161 CD denotes 19
T2836 9157-9158 CD denotes 4
T2837 9158-9159 , denotes ,
T2838 9161-9162 -RRB- denotes ]
T2839 9162-9164 , denotes ,
T2840 9164-9166 PRP denotes we
T2841 9174-9176 IN denotes to
T2842 9177-9187 VBG denotes addressing
T2843 9188-9195 IN denotes whether
T2844 9233-9244 VB denotes participate
T2845 9196-9201 NN denotes Snail
T2846 9202-9206 NN denotes Slug
T2847 9201-9202 HYPH denotes /
T2848 9214-9221 NNS denotes members
T2849 9207-9213 NN denotes family
T2850 9222-9227 MD denotes might
T2851 9228-9232 RB denotes also
T2852 9245-9247 IN denotes in
T2853 9248-9251 DT denotes the
T2854 9252-9259 NN denotes process
T2855 9259-9260 . denotes .
T2856 9260-9415 sentence denotes PCR analyses identified transient Snail mRNA expression during a period of skin embryogenesis when waves of hair follicles are forming (unpublished data).
T2857 9261-9264 NN denotes PCR
T2858 9265-9273 NNS denotes analyses
T2859 9274-9284 VBD denotes identified
T2860 9285-9294 JJ denotes transient
T2861 9306-9316 NN denotes expression
T2862 9295-9300 NN denotes Snail
T2863 9301-9305 NN denotes mRNA
T2864 9317-9323 IN denotes during
T2865 9324-9325 DT denotes a
T2866 9326-9332 NN denotes period
T2867 9333-9335 IN denotes of
T2868 9336-9340 NN denotes skin
T2869 9341-9354 NN denotes embryogenesis
T2870 9355-9359 WRB denotes when
T2871 9388-9395 VBG denotes forming
T2872 9360-9365 NNS denotes waves
T2873 9366-9368 IN denotes of
T2874 9369-9373 NN denotes hair
T2875 9374-9383 NNS denotes follicles
T2876 9384-9387 VBP denotes are
T2877 9396-9397 -LRB- denotes (
T2878 9409-9413 NNS denotes data
T2879 9397-9408 JJ denotes unpublished
T2880 9413-9414 -RRB- denotes )
T2881 9414-9415 . denotes .
T2882 9415-9417 TO denotes To
T2883 9418-9426 VB denotes pinpoint
T2884 9415-9597 sentence denotes To pinpoint specifically where Snail mRNA is expressed in the developing skin, we conducted in situ hybridization using a cRNA probe unique to the Snail 3′ untranslated region (UTR).
T2885 9497-9506 VBD denotes conducted
T2886 9427-9439 RB denotes specifically
T2887 9440-9445 WRB denotes where
T2888 9460-9469 VBN denotes expressed
T2889 9446-9451 NN denotes Snail
T2890 9452-9456 NN denotes mRNA
T2891 9457-9459 VBZ denotes is
T2892 9470-9472 IN denotes in
T2893 9473-9476 DT denotes the
T2894 9488-9492 NN denotes skin
T2895 9477-9487 VBG denotes developing
T2896 9492-9494 , denotes ,
T2897 9494-9496 PRP denotes we
T2898 9507-9509 FW denotes in
T2899 9510-9514 FW denotes situ
T2900 9515-9528 NN denotes hybridization
T2901 9529-9534 VBG denotes using
T2902 9535-9536 DT denotes a
T2903 9542-9547 NN denotes probe
T2904 9537-9541 NN denotes cRNA
T2905 9548-9554 JJ denotes unique
T2906 9555-9557 IN denotes to
T2907 9558-9561 DT denotes the
T2908 9584-9590 NN denotes region
T2909 9562-9567 NNP denotes Snail
T2910 9568-9569 CD denotes 3
T2911 9569-9570 SYM denotes ′
T2912 9571-9583 JJ denotes untranslated
T2913 9591-9592 -LRB- denotes (
T2914 9592-9595 NN denotes UTR
T2915 9595-9596 -RRB- denotes )
T2916 9596-9597 . denotes .
T2917 9597-9786 sentence denotes Embryonic day 17.5 (E17.5) was chosen, since the multiple waves of follicle morphogenesis occurring at this time enabled us to evaluate Snail expression at different stages of the process.
T2918 9598-9607 JJ denotes Embryonic
T2919 9608-9611 NN denotes day
T2920 9629-9635 VBN denotes chosen
T2921 9612-9616 CD denotes 17.5
T2922 9617-9618 -LRB- denotes (
T2923 9618-9623 NN denotes E17.5
T2924 9623-9624 -RRB- denotes )
T2925 9625-9628 VBD denotes was
T2926 9635-9637 , denotes ,
T2927 9637-9642 IN denotes since
T2928 9711-9718 VBD denotes enabled
T2929 9643-9646 DT denotes the
T2930 9656-9661 NNS denotes waves
T2931 9647-9655 JJ denotes multiple
T2932 9662-9664 IN denotes of
T2933 9665-9673 NN denotes follicle
T2934 9674-9687 NN denotes morphogenesis
T2935 9688-9697 VBG denotes occurring
T2936 9698-9700 IN denotes at
T2937 9701-9705 DT denotes this
T2938 9706-9710 NN denotes time
T2939 9719-9721 PRP denotes us
T2940 9722-9724 TO denotes to
T2941 9725-9733 VB denotes evaluate
T2942 9734-9739 NN denotes Snail
T2943 9740-9750 NN denotes expression
T2944 9751-9753 IN denotes at
T2945 9754-9763 JJ denotes different
T2946 9764-9770 NNS denotes stages
T2947 9771-9773 IN denotes of
T2948 9774-9777 DT denotes the
T2949 9778-9785 NN denotes process
T2950 9785-9786 . denotes .
T2951 9786-9889 sentence denotes As shown in Figure 1A, specific hybridization was detected within the epithelium of nascent hair buds.
T2952 9787-9789 IN denotes As
T2953 9790-9795 VBN denotes shown
T2954 9837-9845 VBN denotes detected
T2955 9796-9798 IN denotes in
T2956 9799-9805 NN denotes Figure
T2957 9806-9808 NN denotes 1A
T2958 9808-9810 , denotes ,
T2959 9810-9818 JJ denotes specific
T2960 9819-9832 NN denotes hybridization
T2961 9833-9836 VBD denotes was
T2962 9846-9852 IN denotes within
T2963 9853-9856 DT denotes the
T2964 9857-9867 NN denotes epithelium
T2965 9868-9870 IN denotes of
T2966 9871-9878 JJ denotes nascent
T2967 9884-9888 NNS denotes buds
T2968 9879-9883 NN denotes hair
T2969 9888-9889 . denotes .
T2970 9889-10043 sentence denotes By contrast, as follicles progressed further through their development (e.g., germ and peg stages), they exhibited no signs of hybridization (Figure 1A).
T2971 9890-9892 IN denotes By
T2972 9995-10004 VBD denotes exhibited
T2973 9893-9901 NN denotes contrast
T2974 9901-9903 , denotes ,
T2975 9903-9905 IN denotes as
T2976 9916-9926 VBD denotes progressed
T2977 9906-9915 NNS denotes follicles
T2978 9927-9934 RBR denotes further
T2979 9935-9942 IN denotes through
T2980 9943-9948 PRP$ denotes their
T2981 9949-9960 NN denotes development
T2982 9961-9962 -LRB- denotes (
T2983 9981-9987 NNS denotes stages
T2984 9962-9966 FW denotes e.g.
T2985 9966-9968 , denotes ,
T2986 9968-9972 NN denotes germ
T2987 9973-9976 CC denotes and
T2988 9977-9980 NN denotes peg
T2989 9987-9988 -RRB- denotes )
T2990 9988-9990 , denotes ,
T2991 9990-9994 PRP denotes they
T2992 10005-10007 DT denotes no
T2993 10008-10013 NNS denotes signs
T2994 10014-10016 IN denotes of
T2995 10017-10030 NN denotes hybridization
T2996 10031-10032 -LRB- denotes (
T2997 10039-10041 NN denotes 1A
T2998 10032-10038 NN denotes Figure
T2999 10041-10042 -RRB- denotes )
T3000 10042-10043 . denotes .
T3001 10043-10250 sentence denotes The transient nature of Snail mRNA expression during follicle development was most apparent in hybridized skin sections containing follicles from two different waves of morphogenesis (as shown in Figure 1).
T3002 10044-10047 DT denotes The
T3003 10058-10064 NN denotes nature
T3004 10048-10057 JJ denotes transient
T3005 10118-10121 VBD denotes was
T3006 10065-10067 IN denotes of
T3007 10068-10073 NN denotes Snail
T3008 10079-10089 NN denotes expression
T3009 10074-10078 NN denotes mRNA
T3010 10090-10096 IN denotes during
T3011 10097-10105 NN denotes follicle
T3012 10106-10117 NN denotes development
T3013 10122-10126 RBS denotes most
T3014 10127-10135 JJ denotes apparent
T3015 10136-10138 IN denotes in
T3016 10139-10149 VBN denotes hybridized
T3017 10155-10163 NNS denotes sections
T3018 10150-10154 NN denotes skin
T3019 10164-10174 VBG denotes containing
T3020 10175-10184 NNS denotes follicles
T3021 10185-10189 IN denotes from
T3022 10190-10193 CD denotes two
T3023 10204-10209 NNS denotes waves
T3024 10194-10203 JJ denotes different
T3025 10210-10212 IN denotes of
T3026 10213-10226 NN denotes morphogenesis
T3027 10227-10228 -LRB- denotes (
T3028 10231-10236 VBN denotes shown
T3029 10228-10230 IN denotes as
T3030 10237-10239 IN denotes in
T3031 10240-10246 NN denotes Figure
T3032 10247-10248 CD denotes 1
T3033 10248-10249 -RRB- denotes )
T3034 10249-10250 . denotes .
T3035 10250-10362 sentence denotes Hybridizing hair buds from a later wave appeared juxtaposed with nonhybridizing follicles from an earlier wave.
T3036 10251-10262 VBG denotes Hybridizing
T3037 10268-10272 NNS denotes buds
T3038 10263-10267 NN denotes hair
T3039 10291-10299 VBD denotes appeared
T3040 10273-10277 IN denotes from
T3041 10278-10279 DT denotes a
T3042 10286-10290 NN denotes wave
T3043 10280-10285 JJ denotes later
T3044 10300-10310 JJ denotes juxtaposed
T3045 10311-10315 IN denotes with
T3046 10316-10330 JJ denotes nonhybridizing
T3047 10331-10340 NNS denotes follicles
T3048 10341-10345 IN denotes from
T3049 10346-10348 DT denotes an
T3050 10357-10361 NN denotes wave
T3051 10349-10356 JJR denotes earlier
T3052 10361-10362 . denotes .
T3053 10362-12983 sentence denotes Figure 1 Snail Is Expressed Exclusively in the Hair Bud during Morphogenesis Embryos were either frozen in OCT embedding compound (A, F, and H) or embedded in paraffin (C, D, E, and G), and then sectioned (8 μm). (A) In situ hybridizations with Snail sense or antisense cRNA probes. Black dotted lines demarcate the basement membrane that separates the epidermis (epi) from dermis (der). Arrows point to Snail RNA expression, restricted to the hair bud stage of follicle morphogenesis. It was not seen in later hair germ or peg stages. (B) Expression of Snail protein coincides with hair development. Protein extracts were prepared from keratinocytes transfected with empty expression vector (K14), containing the K14 promoter or with the vector driving HA-tagged Snail (K14-Snail); or from whole skin from E13.5 to P5 animals, including newborn (nb). Equal amounts of proteins were then resolved by SDS-PAGE through 12% gels and subjected to Western blotting using either an affinity-purified Snail polyclonal antiserum, which we generated, or anti-tubulin (loading control). (C–E) Immunohistochemistry shows expression of Snail protein in the nuclei of cells within the hair and skin. (C) E13.5 skin with a single layered epidermis (epi) shows no Snail expression. (D) The first morphological sign that cells have adopted a hair follicle fate is a cluster of cells called a placode in E16.5 skin. Snail is not expressed at this stage of development. (E) Snail is expressed in the hair bud of E17.5 skin but not in later stages of development such as the germ or peg. (F) Immunofluorescence with anti-Ki67 (green) identifies the proliferating cells of the skin, restricted to the basal layer of the epidermis and developing hair follicles. Anti-β4 int labeling reveals the presence of the hemidesmosomal integrin β4, restricted to the base of cells adhering to the underlying basement membrane. The white dotted line marks the outermost surface of the skin. (G) Immunohistochemistry with pMAPK marks a subset of proliferating cells within the epidermis and hair bud. Anti-pMAPK labeling was consistently robust within the hair bud. (H) Immunofluorescence with anti-laminin 5 (lam5), which demarcates the basement membrane, and anti-E-cadherin (E-cad), a component of AJs. At the leading edge of the growing bud, cell-cell borders show markedly diminished anti-E-cadherin labeling (arrowheads). To determine whether this transient nature of Snail mRNA expression is reflected at the protein level, we generated an antibody against the N-terminal sequence that resides upstream of the more conserved zinc finger domains.
T3054 12759-12761 TO denotes To
T3055 12762-12771 VB denotes determine
T3056 12865-12874 VBD denotes generated
T3057 12772-12779 IN denotes whether
T3058 12830-12839 VBN denotes reflected
T3059 12780-12784 DT denotes this
T3060 12795-12801 NN denotes nature
T3061 12785-12794 JJ denotes transient
T3062 12802-12804 IN denotes of
T3063 12805-12810 NN denotes Snail
T3064 12816-12826 NN denotes expression
T3065 12811-12815 NN denotes mRNA
T3066 12827-12829 VBZ denotes is
T3067 12840-12842 IN denotes at
T3068 12843-12846 DT denotes the
T3069 12855-12860 NN denotes level
T3070 12847-12854 NN denotes protein
T3071 12860-12862 , denotes ,
T3072 12862-12864 PRP denotes we
T3073 12875-12877 DT denotes an
T3074 12878-12886 NN denotes antibody
T3075 12887-12894 IN denotes against
T3076 12895-12898 DT denotes the
T3077 12910-12918 NN denotes sequence
T3078 12899-12900 NN denotes N
T3079 12901-12909 JJ denotes terminal
T3080 12900-12901 HYPH denotes -
T3081 12919-12923 WDT denotes that
T3082 12924-12931 VBZ denotes resides
T3083 12932-12940 RB denotes upstream
T3084 12941-12943 IN denotes of
T3085 12944-12947 DT denotes the
T3086 12975-12982 NNS denotes domains
T3087 12948-12952 RBR denotes more
T3088 12953-12962 VBN denotes conserved
T3089 12963-12967 NN denotes zinc
T3090 12968-12974 NN denotes finger
T3091 12982-12983 . denotes .
T3092 12983-13238 sentence denotes As judged by Western blot analysis, the antibody did not detect endogenous proteins from cultured keratinocytes, but it did yield a band of the expected size from keratinocytes transiently expressing a hemagglutinin (HA)-tagged Snail protein (Figure 1B).
T3093 12984-12986 IN denotes As
T3094 12987-12993 VBN denotes judged
T3095 13041-13047 VB denotes detect
T3096 12994-12996 IN denotes by
T3097 12997-13004 NNP denotes Western
T3098 13005-13009 NN denotes blot
T3099 13010-13018 NN denotes analysis
T3100 13018-13020 , denotes ,
T3101 13020-13023 DT denotes the
T3102 13024-13032 NN denotes antibody
T3103 13033-13036 VBD denotes did
T3104 13037-13040 RB denotes not
T3105 13048-13058 JJ denotes endogenous
T3106 13059-13067 NN denotes proteins
T3107 13068-13072 IN denotes from
T3108 13073-13081 VBN denotes cultured
T3109 13082-13095 NNS denotes keratinocytes
T3110 13095-13097 , denotes ,
T3111 13097-13100 CC denotes but
T3112 13101-13103 PRP denotes it
T3113 13108-13113 VB denotes yield
T3114 13104-13107 VBD denotes did
T3115 13114-13115 DT denotes a
T3116 13116-13120 NN denotes band
T3117 13121-13123 IN denotes of
T3118 13124-13127 DT denotes the
T3119 13137-13141 NN denotes size
T3120 13128-13136 VBN denotes expected
T3121 13142-13146 IN denotes from
T3122 13147-13160 NNS denotes keratinocytes
T3123 13161-13172 RB denotes transiently
T3124 13173-13183 VBG denotes expressing
T3125 13184-13185 DT denotes a
T3126 13218-13225 NN denotes protein
T3127 13186-13199 NN denotes hemagglutinin
T3128 13205-13211 VBN denotes tagged
T3129 13200-13201 -LRB- denotes (
T3130 13201-13203 NN denotes HA
T3131 13203-13204 -RRB- denotes )
T3132 13204-13205 HYPH denotes -
T3133 13212-13217 NN denotes Snail
T3134 13226-13227 -LRB- denotes (
T3135 13234-13236 NN denotes 1B
T3136 13227-13233 NN denotes Figure
T3137 13236-13237 -RRB- denotes )
T3138 13237-13238 . denotes .
T3139 13238-13555 sentence denotes The antibody also recognized a band corresponding to the size of endogenous Snail (approximately 28 kDa) in lysates from embryonic mouse skin, the temporal appearance of which corresponded to the waves of hair follicle morphogenesis from E15.5 to newborn when over 90% of the hair on the mouse is formed (Figure 1B).
T3140 13239-13242 DT denotes The
T3141 13243-13251 NN denotes antibody
T3142 13257-13267 VBD denotes recognized
T3143 13252-13256 RB denotes also
T3144 13268-13269 DT denotes a
T3145 13270-13274 NN denotes band
T3146 13275-13288 VBG denotes corresponding
T3147 13289-13291 IN denotes to
T3148 13292-13295 DT denotes the
T3149 13296-13300 NN denotes size
T3150 13301-13303 IN denotes of
T3151 13304-13314 JJ denotes endogenous
T3152 13315-13320 NN denotes Snail
T3153 13321-13322 -LRB- denotes (
T3154 13322-13335 RB denotes approximately
T3155 13336-13338 CD denotes 28
T3156 13339-13342 NN denotes kDa
T3157 13342-13343 -RRB- denotes )
T3158 13344-13346 IN denotes in
T3159 13347-13354 NNS denotes lysates
T3160 13355-13359 IN denotes from
T3161 13360-13369 JJ denotes embryonic
T3162 13376-13380 NN denotes skin
T3163 13370-13375 NN denotes mouse
T3164 13380-13382 , denotes ,
T3165 13382-13385 DT denotes the
T3166 13395-13405 NN denotes appearance
T3167 13386-13394 JJ denotes temporal
T3168 13415-13427 VBD denotes corresponded
T3169 13406-13408 IN denotes of
T3170 13409-13414 WDT denotes which
T3171 13428-13430 IN denotes to
T3172 13431-13434 DT denotes the
T3173 13435-13440 NNS denotes waves
T3174 13441-13443 IN denotes of
T3175 13444-13448 NN denotes hair
T3176 13449-13457 NN denotes follicle
T3177 13458-13471 NN denotes morphogenesis
T3178 13472-13476 IN denotes from
T3179 13477-13482 NN denotes E15.5
T3180 13483-13485 IN denotes to
T3181 13486-13493 JJ denotes newborn
T3182 13494-13498 WRB denotes when
T3183 13536-13542 VBN denotes formed
T3184 13499-13503 IN denotes over
T3185 13504-13506 CD denotes 90
T3186 13506-13507 NN denotes %
T3187 13508-13510 IN denotes of
T3188 13511-13514 DT denotes the
T3189 13515-13519 NN denotes hair
T3190 13520-13522 IN denotes on
T3191 13523-13526 DT denotes the
T3192 13527-13532 NN denotes mouse
T3193 13533-13535 VBZ denotes is
T3194 13543-13544 -LRB- denotes (
T3195 13551-13553 NN denotes 1B
T3196 13544-13550 NN denotes Figure
T3197 13553-13554 -RRB- denotes )
T3198 13554-13555 . denotes .
T3199 13555-13849 sentence denotes Consistent with the Western blot data, immunohistochemical analysis did not detect Snail in single-layered E13.5 epidermis (Figure 1C) nor in the placode, which is the earliest morphological sign of the commitment of multipotent cells of the embryonic ectoderm to a hair cell fate (Figure 1D).
T3200 13556-13566 JJ denotes Consistent
T3201 13632-13638 VB denotes detect
T3202 13567-13571 IN denotes with
T3203 13572-13575 DT denotes the
T3204 13589-13593 NNS denotes data
T3205 13576-13583 NNP denotes Western
T3206 13584-13588 NN denotes blot
T3207 13593-13595 , denotes ,
T3208 13595-13614 JJ denotes immunohistochemical
T3209 13615-13623 NN denotes analysis
T3210 13624-13627 VBD denotes did
T3211 13628-13631 RB denotes not
T3212 13639-13644 NN denotes Snail
T3213 13645-13647 IN denotes in
T3214 13648-13654 JJ denotes single
T3215 13655-13662 JJ denotes layered
T3216 13654-13655 HYPH denotes -
T3217 13669-13678 NN denotes epidermis
T3218 13663-13668 NN denotes E13.5
T3219 13679-13680 -LRB- denotes (
T3220 13687-13689 NN denotes 1C
T3221 13680-13686 NN denotes Figure
T3222 13689-13690 -RRB- denotes )
T3223 13691-13694 CC denotes nor
T3224 13695-13697 IN denotes in
T3225 13698-13701 DT denotes the
T3226 13702-13709 NN denotes placode
T3227 13709-13711 , denotes ,
T3228 13711-13716 WDT denotes which
T3229 13717-13719 VBZ denotes is
T3230 13720-13723 DT denotes the
T3231 13747-13751 NN denotes sign
T3232 13724-13732 JJS denotes earliest
T3233 13733-13746 JJ denotes morphological
T3234 13752-13754 IN denotes of
T3235 13755-13758 DT denotes the
T3236 13759-13769 NN denotes commitment
T3237 13770-13772 IN denotes of
T3238 13773-13784 JJ denotes multipotent
T3239 13785-13790 NNS denotes cells
T3240 13791-13793 IN denotes of
T3241 13794-13797 DT denotes the
T3242 13808-13816 NN denotes ectoderm
T3243 13798-13807 JJ denotes embryonic
T3244 13817-13819 IN denotes to
T3245 13820-13821 DT denotes a
T3246 13832-13836 NN denotes fate
T3247 13822-13826 NN denotes hair
T3248 13827-13831 NN denotes cell
T3249 13837-13838 -LRB- denotes (
T3250 13845-13847 NN denotes 1D
T3251 13838-13844 NN denotes Figure
T3252 13847-13848 -RRB- denotes )
T3253 13848-13849 . denotes .
T3254 13849-14011 sentence denotes Consistent with the in situ hybridization results, anti-Snail antibody labeled only hair buds and not follicles at more mature stages of development (Figure 1E).
T3255 13850-13860 JJ denotes Consistent
T3256 13921-13928 VBD denotes labeled
T3257 13861-13865 IN denotes with
T3258 13866-13869 DT denotes the
T3259 13892-13899 NNS denotes results
T3260 13870-13872 FW denotes in
T3261 13873-13877 FW denotes situ
T3262 13878-13891 NN denotes hybridization
T3263 13899-13901 , denotes ,
T3264 13901-13911 JJ denotes anti-Snail
T3265 13912-13920 NN denotes antibody
T3266 13929-13933 RB denotes only
T3267 13939-13943 NNS denotes buds
T3268 13934-13938 NN denotes hair
T3269 13944-13947 CC denotes and
T3270 13948-13951 RB denotes not
T3271 13952-13961 NNS denotes follicles
T3272 13962-13964 IN denotes at
T3273 13965-13969 RBR denotes more
T3274 13977-13983 NNS denotes stages
T3275 13970-13976 JJ denotes mature
T3276 13984-13986 IN denotes of
T3277 13987-13998 NN denotes development
T3278 13999-14000 -LRB- denotes (
T3279 14007-14009 NN denotes 1E
T3280 14000-14006 NN denotes Figure
T3281 14009-14010 -RRB- denotes )
T3282 14010-14011 . denotes .
T3283 14011-14099 sentence denotes Taken together, the anti-Snail antibody appeared to be specific for its target protein.
T3284 14012-14017 VBN denotes Taken
T3285 14052-14060 VBD denotes appeared
T3286 14018-14026 RB denotes together
T3287 14026-14028 , denotes ,
T3288 14028-14031 DT denotes the
T3289 14043-14051 NN denotes antibody
T3290 14032-14042 JJ denotes anti-Snail
T3291 14061-14063 TO denotes to
T3292 14064-14066 VB denotes be
T3293 14067-14075 JJ denotes specific
T3294 14076-14079 IN denotes for
T3295 14080-14083 PRP$ denotes its
T3296 14091-14098 NN denotes protein
T3297 14084-14090 NN denotes target
T3298 14098-14099 . denotes .
T3299 14099-14215 sentence denotes It did not detect other Snail family members known to be expressed in keratinocytes and/or skin (unpublished data).
T3300 14100-14102 PRP denotes It
T3301 14111-14117 VB denotes detect
T3302 14103-14106 VBD denotes did
T3303 14107-14110 RB denotes not
T3304 14118-14123 JJ denotes other
T3305 14137-14144 NNS denotes members
T3306 14124-14129 NN denotes Snail
T3307 14130-14136 NN denotes family
T3308 14145-14150 VBN denotes known
T3309 14151-14153 TO denotes to
T3310 14157-14166 VBN denotes expressed
T3311 14154-14156 VB denotes be
T3312 14167-14169 IN denotes in
T3313 14170-14183 NNS denotes keratinocytes
T3314 14184-14187 CC denotes and
T3315 14187-14188 HYPH denotes /
T3316 14188-14190 CC denotes or
T3317 14191-14195 NN denotes skin
T3318 14196-14197 -LRB- denotes (
T3319 14209-14213 NNS denotes data
T3320 14197-14208 JJ denotes unpublished
T3321 14213-14214 -RRB- denotes )
T3322 14214-14215 . denotes .
T3323 14215-14377 sentence denotes Furthermore, the immunohistochemical data paralleled our Snail in situ hybridization data revealing transient Snail expression at the hair bud stage (Figure 1A).
T3324 14216-14227 RB denotes Furthermore
T3325 14258-14268 VBD denotes paralleled
T3326 14227-14229 , denotes ,
T3327 14229-14232 DT denotes the
T3328 14253-14257 NNS denotes data
T3329 14233-14252 JJ denotes immunohistochemical
T3330 14269-14272 PRP$ denotes our
T3331 14301-14305 NNS denotes data
T3332 14273-14278 NN denotes Snail
T3333 14287-14300 NN denotes hybridization
T3334 14279-14281 FW denotes in
T3335 14282-14286 FW denotes situ
T3336 14306-14315 VBG denotes revealing
T3337 14316-14325 JJ denotes transient
T3338 14332-14342 NN denotes expression
T3339 14326-14331 NN denotes Snail
T3340 14343-14345 IN denotes at
T3341 14346-14349 DT denotes the
T3342 14359-14364 NN denotes stage
T3343 14350-14354 NN denotes hair
T3344 14355-14358 NN denotes bud
T3345 14365-14366 -LRB- denotes (
T3346 14373-14375 NN denotes 1A
T3347 14366-14372 NN denotes Figure
T3348 14375-14376 -RRB- denotes )
T3349 14376-14377 . denotes .
T3350 14377-14489 sentence denotes As judged by immunohistochemistry, Snail protein was localized to the nuclei of the hair bud cells (Figure 1E).
T3351 14378-14380 IN denotes As
T3352 14381-14387 VBN denotes judged
T3353 14431-14440 VBN denotes localized
T3354 14388-14390 IN denotes by
T3355 14391-14411 NN denotes immunohistochemistry
T3356 14411-14413 , denotes ,
T3357 14413-14418 NN denotes Snail
T3358 14419-14426 NN denotes protein
T3359 14427-14430 VBD denotes was
T3360 14441-14443 IN denotes to
T3361 14444-14447 DT denotes the
T3362 14448-14454 NNS denotes nuclei
T3363 14455-14457 IN denotes of
T3364 14458-14461 DT denotes the
T3365 14471-14476 NNS denotes cells
T3366 14462-14466 NN denotes hair
T3367 14467-14470 NN denotes bud
T3368 14477-14478 -LRB- denotes (
T3369 14485-14487 NN denotes 1E
T3370 14478-14484 NN denotes Figure
T3371 14487-14488 -RRB- denotes )
T3372 14488-14489 . denotes .
T3373 14489-14585 sentence denotes This feature was consistent with Snail's known function as a transcriptional repressor [12,13].
T3374 14490-14494 DT denotes This
T3375 14495-14502 NN denotes feature
T3376 14503-14506 VBD denotes was
T3377 14507-14517 JJ denotes consistent
T3378 14518-14522 IN denotes with
T3379 14523-14528 NN denotes Snail
T3380 14537-14545 NN denotes function
T3381 14528-14530 POS denotes 's
T3382 14531-14536 VBN denotes known
T3383 14546-14548 IN denotes as
T3384 14549-14550 DT denotes a
T3385 14567-14576 NN denotes repressor
T3386 14551-14566 JJ denotes transcriptional
T3387 14577-14578 -LRB- denotes [
T3388 14581-14583 CD denotes 13
T3389 14578-14580 CD denotes 12
T3390 14580-14581 , denotes ,
T3391 14583-14584 -RRB- denotes ]
T3392 14584-14585 . denotes .
T3393 14585-14697 sentence denotes Additionally, anti-Snail labeling was detected in only three of the four major waves of follicle morphogenesis.
T3394 14586-14598 RB denotes Additionally
T3395 14624-14632 VBN denotes detected
T3396 14598-14600 , denotes ,
T3397 14600-14610 JJ denotes anti-Snail
T3398 14611-14619 NN denotes labeling
T3399 14620-14623 VBD denotes was
T3400 14633-14635 IN denotes in
T3401 14636-14640 RB denotes only
T3402 14654-14658 CD denotes four
T3403 14641-14646 CD denotes three
T3404 14647-14649 IN denotes of
T3405 14650-14653 DT denotes the
T3406 14665-14670 NNS denotes waves
T3407 14659-14664 JJ denotes major
T3408 14671-14673 IN denotes of
T3409 14674-14682 NN denotes follicle
T3410 14683-14696 NN denotes morphogenesis
T3411 14696-14697 . denotes .
T3412 14697-14871 sentence denotes Snail was not found in the buds of guard hairs that are the earliest of all hairs to form (at E13.5), and which constitute less than 5% of the mouse coat (unpublished data).
T3413 14698-14703 NN denotes Snail
T3414 14712-14717 VBN denotes found
T3415 14704-14707 VBD denotes was
T3416 14708-14711 RB denotes not
T3417 14718-14720 IN denotes in
T3418 14721-14724 DT denotes the
T3419 14725-14729 NNS denotes buds
T3420 14730-14732 IN denotes of
T3421 14733-14738 NN denotes guard
T3422 14739-14744 NNS denotes hairs
T3423 14745-14749 WDT denotes that
T3424 14750-14753 VBP denotes are
T3425 14754-14757 DT denotes the
T3426 14758-14766 JJS denotes earliest
T3427 14767-14769 IN denotes of
T3428 14770-14773 DT denotes all
T3429 14774-14779 NNS denotes hairs
T3430 14780-14782 TO denotes to
T3431 14783-14787 VB denotes form
T3432 14788-14789 -LRB- denotes (
T3433 14789-14791 IN denotes at
T3434 14792-14797 NN denotes E13.5
T3435 14797-14798 -RRB- denotes )
T3436 14798-14800 , denotes ,
T3437 14800-14803 CC denotes and
T3438 14804-14809 WDT denotes which
T3439 14810-14820 VBP denotes constitute
T3440 14821-14825 JJR denotes less
T3441 14831-14832 CD denotes 5
T3442 14826-14830 IN denotes than
T3443 14832-14833 NN denotes %
T3444 14834-14836 IN denotes of
T3445 14837-14840 DT denotes the
T3446 14847-14851 NN denotes coat
T3447 14841-14846 NN denotes mouse
T3448 14852-14853 -LRB- denotes (
T3449 14865-14869 NNS denotes data
T3450 14853-14864 JJ denotes unpublished
T3451 14869-14870 -RRB- denotes )
T3452 14870-14871 . denotes .
T3453 14871-15112 sentence denotes As judged by immunofluorescence with antibodies against the proliferating nuclear antigen Ki67, the timing of Snail expression coincided with the stage at which the developing follicle enhanced its proliferation and down-growth (Figure 1F).
T3454 14872-14874 IN denotes As
T3455 14875-14881 VBN denotes judged
T3456 14999-15008 VBD denotes coincided
T3457 14882-14884 IN denotes by
T3458 14885-14903 NN denotes immunofluorescence
T3459 14904-14908 IN denotes with
T3460 14909-14919 NNS denotes antibodies
T3461 14920-14927 IN denotes against
T3462 14928-14931 DT denotes the
T3463 14954-14961 NN denotes antigen
T3464 14932-14945 VBG denotes proliferating
T3465 14946-14953 JJ denotes nuclear
T3466 14962-14966 NN denotes Ki67
T3467 14966-14968 , denotes ,
T3468 14968-14971 DT denotes the
T3469 14972-14978 NN denotes timing
T3470 14979-14981 IN denotes of
T3471 14982-14987 NN denotes Snail
T3472 14988-14998 NN denotes expression
T3473 15009-15013 IN denotes with
T3474 15014-15017 DT denotes the
T3475 15018-15023 NN denotes stage
T3476 15024-15026 IN denotes at
T3477 15057-15065 VBD denotes enhanced
T3478 15027-15032 WDT denotes which
T3479 15033-15036 DT denotes the
T3480 15048-15056 NN denotes follicle
T3481 15037-15047 VBG denotes developing
T3482 15066-15069 PRP$ denotes its
T3483 15070-15083 NN denotes proliferation
T3484 15084-15087 CC denotes and
T3485 15088-15092 JJ denotes down
T3486 15093-15099 NN denotes growth
T3487 15092-15093 HYPH denotes -
T3488 15100-15101 -LRB- denotes (
T3489 15108-15110 NN denotes 1F
T3490 15101-15107 NN denotes Figure
T3491 15110-15111 -RRB- denotes )
T3492 15111-15112 . denotes .
T3493 15112-15334 sentence denotes Immunohistochemistry with antibodies against the active (phosphorylated) form of MAPK (pMAPK) marked a subset of the proliferating (Ki67-positive) cells, and pMAPK-positive cells were enriched in the hair bud (Figure 1G).
T3494 15113-15133 NN denotes Immunohistochemistry
T3495 15207-15213 VBD denotes marked
T3496 15134-15138 IN denotes with
T3497 15139-15149 NNS denotes antibodies
T3498 15150-15157 IN denotes against
T3499 15158-15161 DT denotes the
T3500 15186-15190 NN denotes form
T3501 15162-15168 JJ denotes active
T3502 15169-15170 -LRB- denotes (
T3503 15170-15184 VBN denotes phosphorylated
T3504 15184-15185 -RRB- denotes )
T3505 15191-15193 IN denotes of
T3506 15194-15198 NN denotes MAPK
T3507 15199-15200 -LRB- denotes (
T3508 15200-15205 NN denotes pMAPK
T3509 15205-15206 -RRB- denotes )
T3510 15214-15215 DT denotes a
T3511 15216-15222 NN denotes subset
T3512 15223-15225 IN denotes of
T3513 15226-15229 DT denotes the
T3514 15260-15265 NNS denotes cells
T3515 15230-15243 VBG denotes proliferating
T3516 15244-15245 -LRB- denotes (
T3517 15245-15249 NN denotes Ki67
T3518 15250-15258 JJ denotes positive
T3519 15249-15250 HYPH denotes -
T3520 15258-15259 -RRB- denotes )
T3521 15265-15267 , denotes ,
T3522 15267-15270 CC denotes and
T3523 15271-15276 NN denotes pMAPK
T3524 15277-15285 JJ denotes positive
T3525 15276-15277 HYPH denotes -
T3526 15286-15291 NNS denotes cells
T3527 15297-15305 VBN denotes enriched
T3528 15292-15296 VBD denotes were
T3529 15306-15308 IN denotes in
T3530 15309-15312 DT denotes the
T3531 15318-15321 NN denotes bud
T3532 15313-15317 NN denotes hair
T3533 15322-15323 -LRB- denotes (
T3534 15330-15332 NN denotes 1G
T3535 15323-15329 NN denotes Figure
T3536 15332-15333 -RRB- denotes )
T3537 15333-15334 . denotes .
T3538 15334-15530 sentence denotes The timing of Snail induction and Ki67 and pMAPK enrichment in the hair bud appeared to follow closely the induction of LEF-1/β-catenin activity, known to initiate in the hair placode stage [20].
T3539 15335-15338 DT denotes The
T3540 15339-15345 NN denotes timing
T3541 15411-15419 VBD denotes appeared
T3542 15346-15348 IN denotes of
T3543 15349-15354 NN denotes Snail
T3544 15355-15364 NN denotes induction
T3545 15365-15368 CC denotes and
T3546 15369-15373 NN denotes Ki67
T3547 15384-15394 NN denotes enrichment
T3548 15374-15377 CC denotes and
T3549 15378-15383 NN denotes pMAPK
T3550 15395-15397 IN denotes in
T3551 15398-15401 DT denotes the
T3552 15407-15410 NN denotes bud
T3553 15402-15406 NN denotes hair
T3554 15420-15422 TO denotes to
T3555 15423-15429 VB denotes follow
T3556 15430-15437 RB denotes closely
T3557 15438-15441 DT denotes the
T3558 15442-15451 NN denotes induction
T3559 15452-15454 IN denotes of
T3560 15455-15458 NN denotes LEF
T3561 15471-15479 NN denotes activity
T3562 15458-15459 HYPH denotes -
T3563 15459-15460 CD denotes 1
T3564 15460-15461 HYPH denotes /
T3565 15461-15462 NN denotes β
T3566 15463-15470 NN denotes catenin
T3567 15462-15463 HYPH denotes -
T3568 15479-15481 , denotes ,
T3569 15481-15486 VBN denotes known
T3570 15487-15489 TO denotes to
T3571 15490-15498 VB denotes initiate
T3572 15499-15501 IN denotes in
T3573 15502-15505 DT denotes the
T3574 15519-15524 NN denotes stage
T3575 15506-15510 NN denotes hair
T3576 15511-15518 NN denotes placode
T3577 15525-15526 -LRB- denotes [
T3578 15526-15528 CD denotes 20
T3579 15528-15529 -RRB- denotes ]
T3580 15529-15530 . denotes .
T3581 15530-15642 sentence denotes However, like placodes, hair buds exhibited down-regulation in E-cadherin expression (Figure 1H; see also [4]).
T3582 15531-15538 RB denotes However
T3583 15565-15574 VBD denotes exhibited
T3584 15538-15540 , denotes ,
T3585 15540-15544 IN denotes like
T3586 15545-15553 NNS denotes placodes
T3587 15553-15555 , denotes ,
T3588 15555-15559 NN denotes hair
T3589 15560-15564 NNS denotes buds
T3590 15575-15579 JJ denotes down
T3591 15580-15590 NN denotes regulation
T3592 15579-15580 HYPH denotes -
T3593 15591-15593 IN denotes in
T3594 15594-15595 NN denotes E
T3595 15596-15604 NN denotes cadherin
T3596 15595-15596 HYPH denotes -
T3597 15605-15615 NN denotes expression
T3598 15616-15617 -LRB- denotes (
T3599 15624-15626 NN denotes 1H
T3600 15617-15623 NN denotes Figure
T3601 15626-15627 : denotes ;
T3602 15628-15631 VB denotes see
T3603 15632-15636 RB denotes also
T3604 15637-15638 -LRB- denotes [
T3605 15638-15639 CD denotes 4
T3606 15639-15640 -RRB- denotes ]
T3607 15640-15641 -RRB- denotes )
T3608 15641-15642 . denotes .
T3910 15644-15653 JJ denotes Sustained
T3911 15654-15664 NN denotes Expression
T3912 15674-15681 VBZ denotes Results
T3913 15665-15667 IN denotes of
T3914 15668-15673 NN denotes Snail
T3915 15682-15684 IN denotes in
T3916 15685-15694 JJ denotes Epidermal
T3917 15695-15713 NN denotes Hyperproliferation
T3918 15714-15717 CC denotes and
T3919 15718-15733 NN denotes Differentiation
T3920 15734-15741 NNS denotes Defects
T3921 15742-15744 IN denotes in
T3922 15745-15747 NN denotes Tg
T3923 15754-15758 NN denotes Skin
T3924 15748-15753 NN denotes Mouse
T3925 15758-15966 sentence denotes The striking spike of Snail expression coincident with hair bud formation and enhanced proliferation prompted us to examine the consequences of ectopically expressing Snail elsewhere in mouse skin epidermis.
T3926 15759-15762 DT denotes The
T3927 15772-15777 NN denotes spike
T3928 15763-15771 JJ denotes striking
T3929 15860-15868 VBD denotes prompted
T3930 15778-15780 IN denotes of
T3931 15781-15786 NN denotes Snail
T3932 15787-15797 NN denotes expression
T3933 15798-15808 JJ denotes coincident
T3934 15809-15813 IN denotes with
T3935 15814-15818 NN denotes hair
T3936 15819-15822 NN denotes bud
T3937 15823-15832 NN denotes formation
T3938 15833-15836 CC denotes and
T3939 15837-15845 VBN denotes enhanced
T3940 15846-15859 NN denotes proliferation
T3941 15869-15871 PRP denotes us
T3942 15872-15874 TO denotes to
T3943 15875-15882 VB denotes examine
T3944 15883-15886 DT denotes the
T3945 15887-15899 NNS denotes consequences
T3946 15900-15902 IN denotes of
T3947 15903-15914 RB denotes ectopically
T3948 15915-15925 VBG denotes expressing
T3949 15926-15931 NN denotes Snail
T3950 15932-15941 RB denotes elsewhere
T3951 15942-15944 IN denotes in
T3952 15945-15950 NN denotes mouse
T3953 15956-15965 NN denotes epidermis
T3954 15951-15955 NN denotes skin
T3955 15965-15966 . denotes .
T3956 15966-16100 sentence denotes To distinguish Tg from endogenous Snail, we used the HA-epitope, shown previously not to alter Snail's transcriptional activity [12].
T3957 15967-15969 TO denotes To
T3958 15970-15981 VB denotes distinguish
T3959 16011-16015 VBD denotes used
T3960 15982-15984 NN denotes Tg
T3961 15985-15989 IN denotes from
T3962 15990-16000 JJ denotes endogenous
T3963 16001-16006 NN denotes Snail
T3964 16006-16008 , denotes ,
T3965 16008-16010 PRP denotes we
T3966 16016-16019 DT denotes the
T3967 16023-16030 NN denotes epitope
T3968 16020-16022 NN denotes HA
T3969 16022-16023 HYPH denotes -
T3970 16030-16032 , denotes ,
T3971 16032-16037 VBN denotes shown
T3972 16038-16048 RB denotes previously
T3973 16049-16052 RB denotes not
T3974 16056-16061 VB denotes alter
T3975 16053-16055 TO denotes to
T3976 16062-16067 NNP denotes Snail
T3977 16086-16094 NN denotes activity
T3978 16067-16069 POS denotes 's
T3979 16070-16085 JJ denotes transcriptional
T3980 16095-16096 -LRB- denotes [
T3981 16096-16098 CD denotes 12
T3982 16098-16099 -RRB- denotes ]
T3983 16099-16100 . denotes .
T3984 16100-16212 sentence denotes Of 20 K14-Snail[HA] Tg animals generated, three expressed the transgene and all exhibited analogous phenotypes.
T3985 16101-16103 IN denotes Of
T3986 16149-16158 VBD denotes expressed
T3987 16104-16106 CD denotes 20
T3988 16124-16131 NNS denotes animals
T3989 16107-16110 NN denotes K14
T3990 16111-16116 NN denotes Snail
T3991 16110-16111 HYPH denotes -
T3992 16116-16117 -LRB- denotes [
T3993 16117-16119 NN denotes HA
T3994 16119-16120 -RRB- denotes ]
T3995 16121-16123 NN denotes Tg
T3996 16132-16141 VBN denotes generated
T3997 16141-16143 , denotes ,
T3998 16143-16148 CD denotes three
T3999 16159-16162 DT denotes the
T4000 16163-16172 NN denotes transgene
T4001 16173-16176 CC denotes and
T4002 16177-16180 DT denotes all
T4003 16181-16190 VBD denotes exhibited
T4004 16191-16200 JJ denotes analogous
T4005 16201-16211 NNS denotes phenotypes
T4006 16211-16212 . denotes .
T4007 16212-16304 sentence denotes Mice that integrated the transgene at the single-cell stage died at or shortly after birth.
T4008 16213-16217 NNS denotes Mice
T4009 16273-16277 VBD denotes died
T4010 16218-16222 WDT denotes that
T4011 16223-16233 VBD denotes integrated
T4012 16234-16237 DT denotes the
T4013 16238-16247 NN denotes transgene
T4014 16248-16250 IN denotes at
T4015 16251-16254 DT denotes the
T4016 16267-16272 NN denotes stage
T4017 16255-16261 JJ denotes single
T4018 16262-16266 NN denotes cell
T4019 16261-16262 HYPH denotes -
T4020 16278-16280 IN denotes at
T4021 16281-16283 CC denotes or
T4022 16284-16291 RB denotes shortly
T4023 16292-16297 IN denotes after
T4024 16298-16303 NN denotes birth
T4025 16303-16304 . denotes .
T4026 16304-16461 sentence denotes The three surviving full-Tg founder mice harbored transgene integrations that gave stable transmission of mosaic Snail gene expression through the germline.
T4027 16305-16308 DT denotes The
T4028 16341-16345 NNS denotes mice
T4029 16309-16314 CD denotes three
T4030 16315-16324 VBG denotes surviving
T4031 16325-16329 JJ denotes full
T4032 16330-16332 NN denotes Tg
T4033 16329-16330 HYPH denotes -
T4034 16333-16340 NN denotes founder
T4035 16346-16354 VBD denotes harbored
T4036 16355-16364 NN denotes transgene
T4037 16365-16377 NNS denotes integrations
T4038 16378-16382 WDT denotes that
T4039 16383-16387 VBD denotes gave
T4040 16388-16394 JJ denotes stable
T4041 16395-16407 NN denotes transmission
T4042 16408-16410 IN denotes of
T4043 16411-16417 JJ denotes mosaic
T4044 16424-16428 NN denotes gene
T4045 16418-16423 NN denotes Snail
T4046 16429-16439 NN denotes expression
T4047 16440-16447 IN denotes through
T4048 16448-16451 DT denotes the
T4049 16452-16460 NN denotes germline
T4050 16460-16461 . denotes .
T4051 16461-16572 sentence denotes Progressively poor health necessitated our sacrificing most offspring from these lines within a year of birth.
T4052 16462-16475 RB denotes Progressively
T4053 16476-16480 JJ denotes poor
T4054 16481-16487 NN denotes health
T4055 16488-16500 VBD denotes necessitated
T4056 16501-16504 PRP$ denotes our
T4057 16505-16516 VBG denotes sacrificing
T4058 16517-16521 JJS denotes most
T4059 16522-16531 NN denotes offspring
T4060 16532-16536 IN denotes from
T4061 16537-16542 DT denotes these
T4062 16543-16548 NNS denotes lines
T4063 16549-16555 IN denotes within
T4064 16556-16557 DT denotes a
T4065 16558-16562 NN denotes year
T4066 16563-16565 IN denotes of
T4067 16566-16571 NN denotes birth
T4068 16571-16572 . denotes .
T4069 16572-16686 sentence denotes As Snail Tg animals grew, they became distinguished by their small size, short tails, and flaky skin (Figure 2A).
T4070 16573-16575 IN denotes As
T4071 16593-16597 VBD denotes grew
T4072 16576-16581 NN denotes Snail
T4073 16585-16592 NNS denotes animals
T4074 16582-16584 NN denotes Tg
T4075 16604-16610 VBD denotes became
T4076 16597-16599 , denotes ,
T4077 16599-16603 PRP denotes they
T4078 16611-16624 JJ denotes distinguished
T4079 16625-16627 IN denotes by
T4080 16628-16633 PRP$ denotes their
T4081 16640-16644 NN denotes size
T4082 16634-16639 JJ denotes small
T4083 16644-16646 , denotes ,
T4084 16646-16651 JJ denotes short
T4085 16652-16657 NNS denotes tails
T4086 16657-16659 , denotes ,
T4087 16659-16662 CC denotes and
T4088 16663-16668 JJ denotes flaky
T4089 16669-16673 NN denotes skin
T4090 16674-16675 -LRB- denotes (
T4091 16682-16684 NN denotes 2A
T4092 16675-16681 NN denotes Figure
T4093 16684-16685 -RRB- denotes )
T4094 16685-16686 . denotes .
T4095 16686-16797 sentence denotes Histological analyses of 3-d old (P3) mice revealed mosaic patches marked by epidermal thickening (Figure 2B).
T4096 16687-16699 JJ denotes Histological
T4097 16700-16708 NNS denotes analyses
T4098 16730-16738 VBD denotes revealed
T4099 16709-16711 IN denotes of
T4100 16712-16713 CD denotes 3
T4101 16714-16715 NN denotes d
T4102 16713-16714 HYPH denotes -
T4103 16716-16719 JJ denotes old
T4104 16725-16729 NNS denotes mice
T4105 16720-16721 -LRB- denotes (
T4106 16721-16723 JJ denotes P3
T4107 16723-16724 -RRB- denotes )
T4108 16739-16745 NN denotes mosaic
T4109 16746-16753 NNS denotes patches
T4110 16754-16760 VBN denotes marked
T4111 16761-16763 IN denotes by
T4112 16764-16773 JJ denotes epidermal
T4113 16774-16784 NN denotes thickening
T4114 16785-16786 -LRB- denotes (
T4115 16793-16795 NN denotes 2B
T4116 16786-16792 NN denotes Figure
T4117 16795-16796 -RRB- denotes )
T4118 16796-16797 . denotes .
T4119 16797-16952 sentence denotes The mosaic morphology was reflected at the level of Tg Snail protein, with only the hyperthickened regions expressing nuclear HA-tagged Snail (Figure 2C).
T4120 16798-16801 DT denotes The
T4121 16809-16819 NN denotes morphology
T4122 16802-16808 NN denotes mosaic
T4123 16824-16833 VBN denotes reflected
T4124 16820-16823 VBD denotes was
T4125 16834-16836 IN denotes at
T4126 16837-16840 DT denotes the
T4127 16841-16846 NN denotes level
T4128 16847-16849 IN denotes of
T4129 16850-16852 NN denotes Tg
T4130 16859-16866 NN denotes protein
T4131 16853-16858 NN denotes Snail
T4132 16866-16868 , denotes ,
T4133 16868-16872 IN denotes with
T4134 16905-16915 VBG denotes expressing
T4135 16873-16877 RB denotes only
T4136 16897-16904 NNS denotes regions
T4137 16878-16881 DT denotes the
T4138 16882-16896 VBN denotes hyperthickened
T4139 16916-16923 JJ denotes nuclear
T4140 16934-16939 NN denotes Snail
T4141 16924-16926 NN denotes HA
T4142 16927-16933 VBN denotes tagged
T4143 16926-16927 HYPH denotes -
T4144 16940-16941 -LRB- denotes (
T4145 16948-16950 NN denotes 2C
T4146 16941-16947 NN denotes Figure
T4147 16950-16951 -RRB- denotes )
T4148 16951-16952 . denotes .
T4149 16952-17112 sentence denotes These hyperthickened areas were marked by excessive proliferation, as revealed by antibodies against the proliferating nuclear antigen Ki67 (Figure 2D and 2E).
T4150 16953-16958 DT denotes These
T4151 16974-16979 NNS denotes areas
T4152 16959-16973 VBN denotes hyperthickened
T4153 16985-16991 VBN denotes marked
T4154 16980-16984 VBD denotes were
T4155 16992-16994 IN denotes by
T4156 16995-17004 JJ denotes excessive
T4157 17005-17018 NN denotes proliferation
T4158 17018-17020 , denotes ,
T4159 17020-17022 IN denotes as
T4160 17023-17031 VBN denotes revealed
T4161 17032-17034 IN denotes by
T4162 17035-17045 NNS denotes antibodies
T4163 17046-17053 IN denotes against
T4164 17054-17057 DT denotes the
T4165 17080-17087 NN denotes antigen
T4166 17058-17071 VBG denotes proliferating
T4167 17072-17079 JJ denotes nuclear
T4168 17088-17092 NN denotes Ki67
T4169 17093-17094 -LRB- denotes (
T4170 17101-17103 NN denotes 2D
T4171 17094-17100 NN denotes Figure
T4172 17104-17107 CC denotes and
T4173 17108-17110 NN denotes 2E
T4174 17110-17111 -RRB- denotes )
T4175 17111-17112 . denotes .
T4176 17112-17325 sentence denotes Activated, pMAPK-positive cells were also prevalent in these areas (Figure 2F and 2G), as were cells expressing keratin 6, a keratin induced in the suprabasal layers of hyperproliferative skin (Figure 2H and 2I).
T4177 17113-17122 VBN denotes Activated
T4178 17139-17144 NNS denotes cells
T4179 17122-17124 , denotes ,
T4180 17124-17129 NN denotes pMAPK
T4181 17130-17138 JJ denotes positive
T4182 17129-17130 HYPH denotes -
T4183 17145-17149 VBD denotes were
T4184 17150-17154 RB denotes also
T4185 17155-17164 JJ denotes prevalent
T4186 17165-17167 IN denotes in
T4187 17168-17173 DT denotes these
T4188 17174-17179 NNS denotes areas
T4189 17180-17181 -LRB- denotes (
T4190 17188-17190 NN denotes 2F
T4191 17181-17187 NN denotes Figure
T4192 17191-17194 CC denotes and
T4193 17195-17197 NN denotes 2G
T4194 17197-17198 -RRB- denotes )
T4195 17198-17200 , denotes ,
T4196 17200-17202 IN denotes as
T4197 17203-17207 VBD denotes were
T4198 17208-17213 NNS denotes cells
T4199 17214-17224 VBG denotes expressing
T4200 17225-17232 NN denotes keratin
T4201 17233-17234 CD denotes 6
T4202 17234-17236 , denotes ,
T4203 17236-17237 DT denotes a
T4204 17238-17245 NN denotes keratin
T4205 17246-17253 VBN denotes induced
T4206 17254-17256 IN denotes in
T4207 17257-17260 DT denotes the
T4208 17272-17278 NNS denotes layers
T4209 17261-17271 JJ denotes suprabasal
T4210 17279-17281 IN denotes of
T4211 17282-17300 JJ denotes hyperproliferative
T4212 17301-17305 NN denotes skin
T4213 17306-17307 -LRB- denotes (
T4214 17314-17316 NN denotes 2H
T4215 17307-17313 NN denotes Figure
T4216 17317-17320 CC denotes and
T4217 17321-17323 NN denotes 2I
T4218 17323-17324 -RRB- denotes )
T4219 17324-17325 . denotes .
T4220 17325-19464 sentence denotes Figure 2 Misexpression of Snail in Mouse Skin Epidermis Results in Hyperproliferation Three different surviving Tg founder mice harbored a K14-Snail transgene that was integrated into a locus that resulted in inheritable, mosaic expression of the transgene in skin epidermis. All displayed similar abnormalities, as did their offspring. (A) P16 WT and K14-Snail Tg mice. Insets denote magnified tail segments, which displayed a mosaic, flaky appearance in Tg mice. Size differences appeared with age, and are likely due to K14-promoter activity in the tongue and oral epithelium, resulting in progressive defects and reduced food intake. Hence, skin sections from young (P3) mice were analyzed (B–I). (B) Hematoxylin- and eosin-stained Tg skin section. Double arrows demarcate the border of mosaic histology, with seemingly normal epidermis (epi) and a mature hair follicle (hf) at left and hyperthickened epidermis at right. (C) Immunofluorescence of Tg skin section labeled with antibodies as color-coded on frame. Double arrows demarcate the border of mosaic anti-Snail (green), revealing Snail expression coincident with regions of hyperthickened epidermis (at left) and absent in regions of normal epidermis (at right). (D–I) Sections of P3 WT or Tg skin (affected region) subjected to either immunofluorescence (D, E, H, and I) or immunohistochemistry (F and G) with antibodies as indicated on the panel. Anti-keratin 5 indicates K5, normally restricted to the basal layer of the epidermis; anti-keratin 6 detects keratin 6, expressed in postnatal epidermis under conditions such as wounding, in which hyperproliferation occurs. All other antibodies are as in the legend to Figure 2. Comparison of D and E provide representative examples that illustrate that pMAPK is found in only a subset of all proliferating (Ki67-positive) cells. Note also the presence of Ki67- (E) and pMAPK-positive (G) cells in some suprabasal areas; Ki67-positive cells colabeled with anti-Snail (E). Expression of the Snail transgene did not block terminal differentiation in the hyperproliferative epidermis, but it distorted it markedly (Figure 3A–3H).
T4221 19310-19320 NN denotes Expression
T4222 19352-19357 VB denotes block
T4223 19321-19323 IN denotes of
T4224 19324-19327 DT denotes the
T4225 19334-19343 NN denotes transgene
T4226 19328-19333 NN denotes Snail
T4227 19344-19347 VBD denotes did
T4228 19348-19351 RB denotes not
T4229 19358-19366 JJ denotes terminal
T4230 19367-19382 NN denotes differentiation
T4231 19383-19385 IN denotes in
T4232 19386-19389 DT denotes the
T4233 19409-19418 NN denotes epidermis
T4234 19390-19408 JJ denotes hyperproliferative
T4235 19418-19420 , denotes ,
T4236 19420-19423 CC denotes but
T4237 19424-19426 PRP denotes it
T4238 19427-19436 VBD denotes distorted
T4239 19437-19439 PRP denotes it
T4240 19440-19448 RB denotes markedly
T4241 19449-19450 -LRB- denotes (
T4242 19457-19459 NN denotes 3A
T4243 19450-19456 NN denotes Figure
T4244 19459-19460 SYM denotes –
T4245 19460-19462 NN denotes 3H
T4246 19462-19463 -RRB- denotes )
T4247 19463-19464 . denotes .
T4248 19464-19601 sentence denotes Typical of most hyperproliferating conditions, Snail expression led to a large expansion in layers with spinous and granular morphology.
T4249 19465-19472 JJ denotes Typical
T4250 19529-19532 VBD denotes led
T4251 19473-19475 IN denotes of
T4252 19476-19480 JJS denotes most
T4253 19500-19510 NNS denotes conditions
T4254 19481-19499 NN denotes hyperproliferating
T4255 19510-19512 , denotes ,
T4256 19512-19517 NN denotes Snail
T4257 19518-19528 NN denotes expression
T4258 19533-19535 IN denotes to
T4259 19536-19537 DT denotes a
T4260 19544-19553 NN denotes expansion
T4261 19538-19543 JJ denotes large
T4262 19554-19556 IN denotes in
T4263 19557-19563 NNS denotes layers
T4264 19564-19568 IN denotes with
T4265 19569-19576 JJ denotes spinous
T4266 19590-19600 NN denotes morphology
T4267 19577-19580 CC denotes and
T4268 19581-19589 JJ denotes granular
T4269 19600-19601 . denotes .
T4270 19601-19744 sentence denotes Additionally, however, was a marked and variable expansion of keratin 5 (K5), normally restricted to the innermost basal layer (see Figure 3).
T4271 19602-19614 RB denotes Additionally
T4272 19625-19628 VBD denotes was
T4273 19614-19616 , denotes ,
T4274 19616-19623 RB denotes however
T4275 19623-19625 , denotes ,
T4276 19629-19630 DT denotes a
T4277 19651-19660 NN denotes expansion
T4278 19631-19637 JJ denotes marked
T4279 19638-19641 CC denotes and
T4280 19642-19650 JJ denotes variable
T4281 19661-19663 IN denotes of
T4282 19664-19671 NN denotes keratin
T4283 19672-19673 CD denotes 5
T4284 19674-19675 -LRB- denotes (
T4285 19675-19677 NN denotes K5
T4286 19677-19678 -RRB- denotes )
T4287 19678-19680 , denotes ,
T4288 19680-19688 RB denotes normally
T4289 19689-19699 VBN denotes restricted
T4290 19700-19702 IN denotes to
T4291 19703-19706 DT denotes the
T4292 19723-19728 NN denotes layer
T4293 19707-19716 JJS denotes innermost
T4294 19717-19722 JJ denotes basal
T4295 19729-19730 -LRB- denotes (
T4296 19730-19733 VB denotes see
T4297 19734-19740 NN denotes Figure
T4298 19741-19742 CD denotes 3
T4299 19742-19743 -RRB- denotes )
T4300 19743-19744 . denotes .
T4301 19744-20053 sentence denotes Although the failure of Snail-null mice to develop past gastrulation [21] precluded our ability to study the loss of Snail function in skin development, a good correlation emerged between the expression of Snail protein and the extension of K5, Ki67, and pMAPK suprabasally (compare data in Figures 2 and 3).
T4302 19745-19753 IN denotes Although
T4303 19819-19828 VBD denotes precluded
T4304 19754-19757 DT denotes the
T4305 19758-19765 NN denotes failure
T4306 19766-19768 IN denotes of
T4307 19769-19774 NN denotes Snail
T4308 19780-19784 NNS denotes mice
T4309 19774-19775 HYPH denotes -
T4310 19775-19779 JJ denotes null
T4311 19785-19787 TO denotes to
T4312 19788-19795 VB denotes develop
T4313 19796-19800 JJ denotes past
T4314 19801-19813 NN denotes gastrulation
T4315 19814-19815 -LRB- denotes [
T4316 19815-19817 CD denotes 21
T4317 19817-19818 -RRB- denotes ]
T4318 19917-19924 VBD denotes emerged
T4319 19829-19832 PRP$ denotes our
T4320 19833-19840 NN denotes ability
T4321 19841-19843 TO denotes to
T4322 19844-19849 VB denotes study
T4323 19850-19853 DT denotes the
T4324 19854-19858 NN denotes loss
T4325 19859-19861 IN denotes of
T4326 19862-19867 NN denotes Snail
T4327 19868-19876 NN denotes function
T4328 19877-19879 IN denotes in
T4329 19880-19884 NN denotes skin
T4330 19885-19896 NN denotes development
T4331 19896-19898 , denotes ,
T4332 19898-19899 DT denotes a
T4333 19905-19916 NN denotes correlation
T4334 19900-19904 JJ denotes good
T4335 19925-19932 IN denotes between
T4336 19933-19936 DT denotes the
T4337 19937-19947 NN denotes expression
T4338 19948-19950 IN denotes of
T4339 19951-19956 NN denotes Snail
T4340 19957-19964 NN denotes protein
T4341 19965-19968 CC denotes and
T4342 19969-19972 DT denotes the
T4343 19973-19982 NN denotes extension
T4344 19983-19985 IN denotes of
T4345 19986-19988 NN denotes K5
T4346 19988-19990 , denotes ,
T4347 19990-19994 NN denotes Ki67
T4348 19994-19996 , denotes ,
T4349 19996-19999 CC denotes and
T4350 20000-20005 NN denotes pMAPK
T4351 20006-20018 RB denotes suprabasally
T4352 20019-20020 -LRB- denotes (
T4353 20020-20027 VB denotes compare
T4354 20028-20032 NNS denotes data
T4355 20033-20035 IN denotes in
T4356 20036-20043 NNS denotes Figures
T4357 20044-20045 CD denotes 2
T4358 20046-20049 CC denotes and
T4359 20050-20051 CD denotes 3
T4360 20051-20052 -RRB- denotes )
T4361 20052-20053 . denotes .
T4362 20053-22035 sentence denotes Figure 3 Alterations in the Differentiation Program and Basement Membrane Organization in Snail-Expressing Tg Epidermis (A–H) Immunofluorescence of skin sections from P3 WT and Tg mice. Shown are affected areas of Tg skin; in areas where Snail protein was not expressed, stainings were normal. Sections were labeled with antibodies as indicated and color-coded on each frame. Antibodies are against markers of normal epidermal differentiation, and include K5 (a basally expressed keratin), K1 (a suprabasal keratin, expressed in spinous layer cells), involucrin (Inv; a suprabasally expressed cornified envelope protein found in upper spinous and granular layer cells), loricrin (Lor; a cornified envelope protein expressed in the granular layer), and filaggrin (Fil; a protein that bundles keratin filaments in the granular layer and stratum corneum). Note abnormal extension of anti-K5 suprabasally, often present in anti-K1 positive suprabasal Tg cells. (I–N) Immunohistochemistry (I and J) or immunofluorescence (K–N) of sections of P30 Wt (I, K, and M) and Tg (J, L, and N) (affected areas) skins using the antibodies indicated. Note that with age, affected areas of the Tg epidermis became increasingly undulating, often exhibiting papilloma-like invaginations (J). Insets in I and J are magnified views of the boxed areas, illustrating the absence (Wt) or presence (Tg) of nuclear anti-cyclin D staining. With age, affected areas of the Tg epidermis also displayed perturbations within the basement membrane, as judged by antibody labeling against either basement membrane (K and L) or hemidesmosomal (M and N) components. Double arrows in L demarcate mosaic zones, revealing that perturbations were restricted to hyperthickened, i.e., Snail-positive zones (to left of double arrows). Other abbreviations are as noted in the legend to Figure 2. The changes in hyperproliferation and differentiation were not initially accompanied by gross signs of epithelial invaginations.
T4363 21907-21910 DT denotes The
T4364 21911-21918 NNS denotes changes
T4365 21980-21991 VBN denotes accompanied
T4366 21919-21921 IN denotes in
T4367 21922-21940 NN denotes hyperproliferation
T4368 21941-21944 CC denotes and
T4369 21945-21960 NN denotes differentiation
T4370 21961-21965 VBD denotes were
T4371 21966-21969 RB denotes not
T4372 21970-21979 RB denotes initially
T4373 21992-21994 IN denotes by
T4374 21995-22000 JJ denotes gross
T4375 22001-22006 NNS denotes signs
T4376 22007-22009 IN denotes of
T4377 22010-22020 JJ denotes epithelial
T4378 22021-22034 NNS denotes invaginations
T4379 22034-22035 . denotes .
T4380 22035-22229 sentence denotes With age, however, epidermal folds and undulations developed in areas where Snail was expressed, and proliferative markers persisted in these regions (Figure 3I and 3J; anti-cyclin D staining).
T4381 22036-22040 IN denotes With
T4382 22087-22096 VBN denotes developed
T4383 22041-22044 NN denotes age
T4384 22044-22046 , denotes ,
T4385 22046-22053 RB denotes however
T4386 22053-22055 , denotes ,
T4387 22055-22064 JJ denotes epidermal
T4388 22065-22070 NNS denotes folds
T4389 22071-22074 CC denotes and
T4390 22075-22086 NNS denotes undulations
T4391 22097-22099 IN denotes in
T4392 22100-22105 NNS denotes areas
T4393 22106-22111 WRB denotes where
T4394 22122-22131 VBN denotes expressed
T4395 22112-22117 NN denotes Snail
T4396 22118-22121 VBD denotes was
T4397 22131-22133 , denotes ,
T4398 22133-22136 CC denotes and
T4399 22137-22150 JJ denotes proliferative
T4400 22151-22158 NNS denotes markers
T4401 22159-22168 VBD denotes persisted
T4402 22169-22171 IN denotes in
T4403 22172-22177 DT denotes these
T4404 22178-22185 NNS denotes regions
T4405 22186-22187 -LRB- denotes (
T4406 22219-22227 NN denotes staining
T4407 22187-22193 NN denotes Figure
T4408 22194-22196 NN denotes 3I
T4409 22197-22200 CC denotes and
T4410 22201-22203 NN denotes 3J
T4411 22203-22204 : denotes ;
T4412 22205-22216 JJ denotes anti-cyclin
T4413 22217-22218 NN denotes D
T4414 22227-22228 -RRB- denotes )
T4415 22228-22229 . denotes .
T4416 22229-22341 sentence denotes The undulations were accompanied by partial dissolution of the underlying basement membrane (Figure 3K and 3L).
T4417 22230-22233 DT denotes The
T4418 22234-22245 NNS denotes undulations
T4419 22251-22262 VBN denotes accompanied
T4420 22246-22250 VBD denotes were
T4421 22263-22265 IN denotes by
T4422 22266-22273 JJ denotes partial
T4423 22274-22285 NN denotes dissolution
T4424 22286-22288 IN denotes of
T4425 22289-22292 DT denotes the
T4426 22313-22321 NN denotes membrane
T4427 22293-22303 JJ denotes underlying
T4428 22304-22312 NN denotes basement
T4429 22322-22323 -LRB- denotes (
T4430 22330-22332 NN denotes 3K
T4431 22323-22329 NN denotes Figure
T4432 22333-22336 CC denotes and
T4433 22337-22339 NN denotes 3L
T4434 22339-22340 -RRB- denotes )
T4435 22340-22341 . denotes .
T4436 22341-22541 sentence denotes Aberrant staining was also observed with antibodies against components of the hemidesmosomes, which provide strong adhesion of basal epidermal cells to the underlying basal lamina (Figure 3M and 3N).
T4437 22342-22350 JJ denotes Aberrant
T4438 22351-22359 NN denotes staining
T4439 22369-22377 VBN denotes observed
T4440 22360-22363 VBD denotes was
T4441 22364-22368 RB denotes also
T4442 22378-22382 IN denotes with
T4443 22383-22393 NNS denotes antibodies
T4444 22394-22401 IN denotes against
T4445 22402-22412 NNS denotes components
T4446 22413-22415 IN denotes of
T4447 22416-22419 DT denotes the
T4448 22420-22434 NNS denotes hemidesmosomes
T4449 22434-22436 , denotes ,
T4450 22436-22441 WDT denotes which
T4451 22442-22449 VBP denotes provide
T4452 22450-22456 JJ denotes strong
T4453 22457-22465 NN denotes adhesion
T4454 22466-22468 IN denotes of
T4455 22469-22474 JJ denotes basal
T4456 22485-22490 NNS denotes cells
T4457 22475-22484 JJ denotes epidermal
T4458 22491-22493 IN denotes to
T4459 22494-22497 DT denotes the
T4460 22515-22521 NN denotes lamina
T4461 22498-22508 JJ denotes underlying
T4462 22509-22514 JJ denotes basal
T4463 22522-22523 -LRB- denotes (
T4464 22530-22532 NN denotes 3M
T4465 22523-22529 NN denotes Figure
T4466 22533-22536 CC denotes and
T4467 22537-22539 NN denotes 3N
T4468 22539-22540 -RRB- denotes )
T4469 22540-22541 . denotes .
T4470 22541-22696 sentence denotes Interestingly, similar types of alterations occur in the basement membrane in the hair bud of embryonic and newborn mice when Snail is normally expressed.
T4471 22542-22555 RB denotes Interestingly
T4472 22586-22591 VBP denotes occur
T4473 22555-22557 , denotes ,
T4474 22557-22564 JJ denotes similar
T4475 22565-22570 NNS denotes types
T4476 22571-22573 IN denotes of
T4477 22574-22585 NNS denotes alterations
T4478 22592-22594 IN denotes in
T4479 22595-22598 DT denotes the
T4480 22608-22616 NN denotes membrane
T4481 22599-22607 NN denotes basement
T4482 22617-22619 IN denotes in
T4483 22620-22623 DT denotes the
T4484 22629-22632 NN denotes bud
T4485 22624-22628 NN denotes hair
T4486 22633-22635 IN denotes of
T4487 22636-22645 JJ denotes embryonic
T4488 22658-22662 NNS denotes mice
T4489 22646-22649 CC denotes and
T4490 22650-22657 JJ denotes newborn
T4491 22663-22667 WRB denotes when
T4492 22686-22695 VBN denotes expressed
T4493 22668-22673 NN denotes Snail
T4494 22674-22676 VBZ denotes is
T4495 22677-22685 RB denotes normally
T4496 22695-22696 . denotes .
T4497 22696-22933 sentence denotes The fact that the basement membrane separating the epidermis from the dermis is altered only in the adult Tg animals suggests the involvement of intermediary factors not as readily available in the epidermis as they are in the follicle.
T4498 22697-22700 DT denotes The
T4499 22701-22705 NN denotes fact
T4500 22814-22822 VBZ denotes suggests
T4501 22706-22710 IN denotes that
T4502 22777-22784 VBN denotes altered
T4503 22711-22714 DT denotes the
T4504 22724-22732 NN denotes membrane
T4505 22715-22723 NN denotes basement
T4506 22733-22743 VBG denotes separating
T4507 22744-22747 DT denotes the
T4508 22748-22757 NN denotes epidermis
T4509 22758-22762 IN denotes from
T4510 22763-22766 DT denotes the
T4511 22767-22773 NN denotes dermis
T4512 22774-22776 VBZ denotes is
T4513 22785-22789 RB denotes only
T4514 22790-22792 IN denotes in
T4515 22793-22796 DT denotes the
T4516 22806-22813 NNS denotes animals
T4517 22797-22802 JJ denotes adult
T4518 22803-22805 NN denotes Tg
T4519 22823-22826 DT denotes the
T4520 22827-22838 NN denotes involvement
T4521 22839-22841 IN denotes of
T4522 22842-22854 JJ denotes intermediary
T4523 22855-22862 NNS denotes factors
T4524 22863-22866 RB denotes not
T4525 22878-22887 JJ denotes available
T4526 22867-22869 RB denotes as
T4527 22870-22877 RB denotes readily
T4528 22888-22890 IN denotes in
T4529 22891-22894 DT denotes the
T4530 22895-22904 NN denotes epidermis
T4531 22905-22907 IN denotes as
T4532 22913-22916 VBP denotes are
T4533 22908-22912 PRP denotes they
T4534 22917-22919 IN denotes in
T4535 22920-22923 DT denotes the
T4536 22924-22932 NN denotes follicle
T4537 22932-22933 . denotes .
T5218 22935-22943 JJ denotes Possible
T5219 22944-22949 NNS denotes Links
T5220 22950-22957 IN denotes between
T5221 22958-22967 JJ denotes Epidermal
T5222 22968-22986 NN denotes Hyperproliferation
T5223 22987-22990 CC denotes and
T5224 22991-22995 JJ denotes Down
T5225 22996-23006 NN denotes regulation
T5226 22995-22996 HYPH denotes -
T5227 23007-23009 IN denotes of
T5228 23010-23012 NN denotes AJ
T5229 23013-23021 NNS denotes Proteins
T5230 23022-23024 IN denotes in
T5231 23025-23030 NN denotes Snail
T5232 23034-23038 NNS denotes Mice
T5233 23031-23033 NN denotes Tg
T5234 23038-23358 sentence denotes Given that the E-cadherin promoter is a direct target for Snail-mediated repression in vitro [4,12,13], and that E-cadherin was down-regulated in Snail-expressing hair buds, we examined the status of E-cadherin and other AJ proteins within regions of hyperproliferative epidermis where Tg Snail was present (Figure 4A).
T5235 23039-23044 VBN denotes Given
T5236 23216-23224 VBD denotes examined
T5237 23045-23049 IN denotes that
T5238 23074-23076 VBZ denotes is
T5239 23050-23053 DT denotes the
T5240 23065-23073 NN denotes promoter
T5241 23054-23055 NN denotes E
T5242 23056-23064 NN denotes cadherin
T5243 23055-23056 HYPH denotes -
T5244 23077-23078 DT denotes a
T5245 23086-23092 NN denotes target
T5246 23079-23085 JJ denotes direct
T5247 23093-23096 IN denotes for
T5248 23097-23102 NN denotes Snail
T5249 23103-23111 VBN denotes mediated
T5250 23102-23103 HYPH denotes -
T5251 23112-23122 NN denotes repression
T5252 23123-23125 FW denotes in
T5253 23126-23131 FW denotes vitro
T5254 23132-23133 -LRB- denotes [
T5255 23138-23140 CD denotes 13
T5256 23133-23134 CD denotes 4
T5257 23134-23135 , denotes ,
T5258 23135-23137 CD denotes 12
T5259 23137-23138 , denotes ,
T5260 23140-23141 -RRB- denotes ]
T5261 23141-23143 , denotes ,
T5262 23143-23146 CC denotes and
T5263 23147-23151 IN denotes that
T5264 23172-23181 VBN denotes regulated
T5265 23152-23153 NN denotes E
T5266 23154-23162 NN denotes cadherin
T5267 23153-23154 HYPH denotes -
T5268 23163-23166 VBD denotes was
T5269 23167-23171 RB denotes down
T5270 23171-23172 HYPH denotes -
T5271 23182-23184 IN denotes in
T5272 23185-23190 NN denotes Snail
T5273 23191-23201 VBG denotes expressing
T5274 23190-23191 HYPH denotes -
T5275 23207-23211 NNS denotes buds
T5276 23202-23206 NN denotes hair
T5277 23211-23213 , denotes ,
T5278 23213-23215 PRP denotes we
T5279 23225-23228 DT denotes the
T5280 23229-23235 NN denotes status
T5281 23236-23238 IN denotes of
T5282 23239-23240 NN denotes E
T5283 23241-23249 NN denotes cadherin
T5284 23240-23241 HYPH denotes -
T5285 23250-23253 CC denotes and
T5286 23254-23259 JJ denotes other
T5287 23263-23271 NN denotes proteins
T5288 23260-23262 NN denotes AJ
T5289 23272-23278 IN denotes within
T5290 23279-23286 NNS denotes regions
T5291 23287-23289 IN denotes of
T5292 23290-23308 JJ denotes hyperproliferative
T5293 23309-23318 NN denotes epidermis
T5294 23319-23324 WRB denotes where
T5295 23334-23337 VBD denotes was
T5296 23325-23327 NN denotes Tg
T5297 23328-23333 NN denotes Snail
T5298 23338-23345 JJ denotes present
T5299 23346-23347 -LRB- denotes (
T5300 23354-23356 NN denotes 4A
T5301 23347-23353 NN denotes Figure
T5302 23356-23357 -RRB- denotes )
T5303 23357-23358 . denotes .
T5304 23358-23458 sentence denotes In these regions, immunofluorescence staining of E-cadherin and α-catenin were markedly diminished.
T5305 23359-23361 IN denotes In
T5306 23447-23457 VBN denotes diminished
T5307 23362-23367 DT denotes these
T5308 23368-23375 NNS denotes regions
T5309 23375-23377 , denotes ,
T5310 23377-23395 NN denotes immunofluorescence
T5311 23396-23404 NN denotes staining
T5312 23405-23407 IN denotes of
T5313 23408-23409 NN denotes E
T5314 23410-23418 NN denotes cadherin
T5315 23409-23410 HYPH denotes -
T5316 23419-23422 CC denotes and
T5317 23423-23424 NN denotes α
T5318 23425-23432 NN denotes catenin
T5319 23424-23425 HYPH denotes -
T5320 23433-23437 VBD denotes were
T5321 23438-23446 RB denotes markedly
T5322 23457-23458 . denotes .
T5323 23458-23572 sentence denotes In contrast, the intensity of antibody staining for two other AJ proteins, β-catenin and Ajuba, was still strong.
T5324 23459-23461 IN denotes In
T5325 23555-23558 VBD denotes was
T5326 23462-23470 NN denotes contrast
T5327 23470-23472 , denotes ,
T5328 23472-23475 DT denotes the
T5329 23476-23485 NN denotes intensity
T5330 23486-23488 IN denotes of
T5331 23489-23497 NN denotes antibody
T5332 23498-23506 NN denotes staining
T5333 23507-23510 IN denotes for
T5334 23511-23514 CD denotes two
T5335 23524-23532 NN denotes proteins
T5336 23515-23520 JJ denotes other
T5337 23521-23523 NN denotes AJ
T5338 23532-23534 , denotes ,
T5339 23534-23535 NN denotes β
T5340 23536-23543 NN denotes catenin
T5341 23535-23536 HYPH denotes -
T5342 23544-23547 CC denotes and
T5343 23548-23553 NN denotes Ajuba
T5344 23553-23555 , denotes ,
T5345 23559-23564 RB denotes still
T5346 23565-23571 JJ denotes strong
T5347 23571-23572 . denotes .
T5348 23572-23760 sentence denotes Interestingly, however, despite appreciable immunofluorescence, localization of β-catenin and Ajuba appeared to be largely cytoplasmic rather than at cell-cell borders (Figure 4A insets).
T5349 23573-23586 RB denotes Interestingly
T5350 23673-23681 VBD denotes appeared
T5351 23586-23588 , denotes ,
T5352 23588-23595 RB denotes however
T5353 23595-23597 , denotes ,
T5354 23597-23604 IN denotes despite
T5355 23605-23616 JJ denotes appreciable
T5356 23617-23635 NN denotes immunofluorescence
T5357 23635-23637 , denotes ,
T5358 23637-23649 NN denotes localization
T5359 23650-23652 IN denotes of
T5360 23653-23654 NN denotes β
T5361 23655-23662 NN denotes catenin
T5362 23654-23655 HYPH denotes -
T5363 23663-23666 CC denotes and
T5364 23667-23672 NN denotes Ajuba
T5365 23682-23684 TO denotes to
T5366 23685-23687 VB denotes be
T5367 23688-23695 RB denotes largely
T5368 23696-23707 JJ denotes cytoplasmic
T5369 23708-23714 RB denotes rather
T5370 23715-23719 IN denotes than
T5371 23720-23722 IN denotes at
T5372 23723-23727 NN denotes cell
T5373 23728-23732 NN denotes cell
T5374 23727-23728 HYPH denotes -
T5375 23733-23740 NNS denotes borders
T5376 23741-23742 -LRB- denotes (
T5377 23752-23758 NNS denotes insets
T5378 23742-23748 NN denotes Figure
T5379 23749-23751 NN denotes 4A
T5380 23758-23759 -RRB- denotes )
T5381 23759-23760 . denotes .
T5382 23760-27837 sentence denotes Figure 4 Snail-Mediated Remodeling of AJs Contributes to Hyperproliferation (A) Immunofluorescence of skin sections from P30 Wt and Tg mice. Shown are affected areas of Tg skin; in areas where Snail protein was not expressed, stainings were normal. Antibodies used are against AJ proteins and include E-cadherin (E-cad), the transmembrane core protein; β-catenin (β-cat), which binds E-cadherin at AJs and which can also participate as a transcription cofactor when associated with LEF-1/TCF proteins in the nucleus; α-catenin (α-cat) which binds to both β-catenin and Ajuba, a close relative of zyxin; and Ajuba, which can associate with proteins that bind to the actin cytoskeleton, as well as with Grb-2, a mediator of the GTP nucleotide-exchange protein Sos, involved in activation of the Ras-MAPK signaling cascade. In Snail-expressing Tg regions, there was a reduced staining with anti-E-cad and anti-α-cat and a more diffuse staining with anti-Ajuba. Insets in the panels for β-catenin and Ajuba staining are magnified views of the boxed areas. Arrows mark membrane localization of the protein and asterisks mark cells with elevated levels of cytoplasmic β-catenin or Ajuba. (B) Western blot analyses of protein extracts from P30 Wt and Tg back and ear skins. Antibodies are as in (A) except anti-P-cad, which detects P-cadherin, whose expression in the hair follicle was not affected, and anti-tubulin, which detects tubulin, a control for equal protein loadings. Note that the reductions seen in E-cadherin and α-catenin are likely to be underestimates of the actual differences in affected regions, since the Tg skin expressed Snail mosaically. (C) In the presence of elevated Snail, α-catenin levels can be restored by overexpression of E-cadherin. Keratinocytes were transfected with either HA-tagged Snail (Snail[HA]; images on the left) or Snail(HA) and Ecad(HA) (images on the right). 2 d after transfection, cells were switched from low-calcium growth medium to high-calcium medium for 6 h to induce AJ formation. Cells were stained with antibodies as indicated on the panels. Arrowheads point to sites of intercellular contact between a Snail-transfected keratinocyte and its neighboring untransfected cell. (D) Reintroduction of E-cadherin in keratinocytes expressing Snail returns pMAPK to basal levels. Keratinocytes were transfected with control vector (K14), or Snail(HA), or Snail(HA) + E-cad(HA). After 2 d, cells were serum starved for 4 h and whole cell lysates were made and Western blotted with antibodies to pMAPK, HA to recognize the HA-tagged Snail and E-cadherin protein, 20or tubulin as a loading control. (E) Ajuba interacts with Grb-2 under conditions where α-catenin levels are reduced. Protein extracts were made from skins of P30 Wt and K14-Snail Tg P30 mice (blots on the left) and of newborn Wt and K14-Cre/α-catenin (fl/fl) conditionally null animals (blots on the right) [7]. Equal amounts of protein extracts were treated with anti-Grb-2 antibody (+) or control isotype antibody (–), and following centrifugation, immunoprecipitates were subjected to SDS-PAGE and Western blot analysis with anti-Ajuba and anti-Grb-2 antibodies. Note the presence of Ajuba only under conditions where levels of α-catenin and other AJ proteins were aberrantly low or absent. (F) Transgene expression of excess Ajuba or the Grb-2-interacting domain (pre-LIM) of Ajuba in keratinocytes results in the activation of the Ras-MAPK pathway. Primary newborn mouse keratinocytes were transfected with either the empty K14 expression vector (K14), or the expression vector driving Snail, full length Ajuba, or the pre-LIM domain of Ajuba in the absence or presence of a peptide inhibitor (inh) that disrupts the interaction between Grb-2 and Sos. 48 h posttransfection, protein extracts were prepared and subjected to SDS-PAGE and Western blot analyses with antibodies against pMAPK, total MAPK, Ajuba (also recognizing the smaller, pre-LIM domain), and Snail. Architectural differences in the epidermis made Western blot analyses somewhat difficult to gauge.
T5383 27739-27752 JJ denotes Architectural
T5384 27753-27764 NNS denotes differences
T5385 27782-27786 VBD denotes made
T5386 27765-27767 IN denotes in
T5387 27768-27771 DT denotes the
T5388 27772-27781 NN denotes epidermis
T5389 27787-27794 NNP denotes Western
T5390 27795-27799 NN denotes blot
T5391 27800-27808 NNS denotes analyses
T5392 27818-27827 JJ denotes difficult
T5393 27809-27817 RB denotes somewhat
T5394 27828-27830 TO denotes to
T5395 27831-27836 VB denotes gauge
T5396 27836-27837 . denotes .
T5397 27837-27963 sentence denotes However, in regions such as ear skin, where the highest levels of Snail protein were expressed, the effects were accentuated.
T5398 27838-27845 RB denotes However
T5399 27951-27962 VBN denotes accentuated
T5400 27845-27847 , denotes ,
T5401 27847-27849 IN denotes in
T5402 27850-27857 NNS denotes regions
T5403 27858-27862 JJ denotes such
T5404 27863-27865 IN denotes as
T5405 27866-27869 NN denotes ear
T5406 27870-27874 NN denotes skin
T5407 27874-27876 , denotes ,
T5408 27876-27881 WRB denotes where
T5409 27923-27932 VBN denotes expressed
T5410 27882-27885 DT denotes the
T5411 27894-27900 NNS denotes levels
T5412 27886-27893 JJS denotes highest
T5413 27901-27903 IN denotes of
T5414 27904-27909 NN denotes Snail
T5415 27910-27917 NN denotes protein
T5416 27918-27922 VBD denotes were
T5417 27932-27934 , denotes ,
T5418 27934-27937 DT denotes the
T5419 27938-27945 NNS denotes effects
T5420 27946-27950 VBD denotes were
T5421 27962-27963 . denotes .
T5422 27963-28155 sentence denotes In both back skin and ear skin, overall levels of E-cadherin and α-catenin were reduced, under conditions where β-catenin and Ajuba levels remained unchanged relative to controls (Figure 4B).
T5423 27964-27966 IN denotes In
T5424 28044-28051 VBN denotes reduced
T5425 27967-27971 CC denotes both
T5426 27977-27981 NN denotes skin
T5427 27972-27976 NN denotes back
T5428 27982-27985 CC denotes and
T5429 27986-27989 NN denotes ear
T5430 27990-27994 NN denotes skin
T5431 27994-27996 , denotes ,
T5432 27996-28003 JJ denotes overall
T5433 28004-28010 NNS denotes levels
T5434 28011-28013 IN denotes of
T5435 28014-28015 NN denotes E
T5436 28016-28024 NN denotes cadherin
T5437 28015-28016 HYPH denotes -
T5438 28025-28028 CC denotes and
T5439 28029-28030 NN denotes α
T5440 28031-28038 NN denotes catenin
T5441 28030-28031 HYPH denotes -
T5442 28039-28043 VBD denotes were
T5443 28051-28053 , denotes ,
T5444 28053-28058 IN denotes under
T5445 28059-28069 NNS denotes conditions
T5446 28070-28075 WRB denotes where
T5447 28103-28111 VBD denotes remained
T5448 28076-28077 NN denotes β
T5449 28078-28085 NN denotes catenin
T5450 28077-28078 HYPH denotes -
T5451 28096-28102 NNS denotes levels
T5452 28086-28089 CC denotes and
T5453 28090-28095 NN denotes Ajuba
T5454 28112-28121 JJ denotes unchanged
T5455 28122-28130 JJ denotes relative
T5456 28131-28133 IN denotes to
T5457 28134-28142 NNS denotes controls
T5458 28143-28144 -LRB- denotes (
T5459 28151-28153 NN denotes 4B
T5460 28144-28150 NN denotes Figure
T5461 28153-28154 -RRB- denotes )
T5462 28154-28155 . denotes .
T5463 28155-28260 sentence denotes Taken together, these data were consistent with our results obtained from immunofluorescence microscopy.
T5464 28156-28161 VBN denotes Taken
T5465 28183-28187 VBD denotes were
T5466 28162-28170 RB denotes together
T5467 28170-28172 , denotes ,
T5468 28172-28177 DT denotes these
T5469 28178-28182 NNS denotes data
T5470 28188-28198 JJ denotes consistent
T5471 28199-28203 IN denotes with
T5472 28204-28207 PRP$ denotes our
T5473 28208-28215 NNS denotes results
T5474 28216-28224 VBN denotes obtained
T5475 28225-28229 IN denotes from
T5476 28230-28248 NN denotes immunofluorescence
T5477 28249-28259 NN denotes microscopy
T5478 28259-28260 . denotes .
T5479 28260-28456 sentence denotes A priori, the decrease in α-catenin levels could be due to either direct transcriptional repression by Snail or perturbations in AJ formation caused by the decrease in E-cadherin gene expression.
T5480 28261-28262 FW denotes A
T5481 28263-28269 FW denotes priori
T5482 28310-28312 VB denotes be
T5483 28269-28271 , denotes ,
T5484 28271-28274 DT denotes the
T5485 28275-28283 NN denotes decrease
T5486 28284-28286 IN denotes in
T5487 28287-28288 NN denotes α
T5488 28289-28296 NN denotes catenin
T5489 28288-28289 HYPH denotes -
T5490 28297-28303 NNS denotes levels
T5491 28304-28309 MD denotes could
T5492 28313-28316 IN denotes due
T5493 28317-28319 IN denotes to
T5494 28320-28326 CC denotes either
T5495 28350-28360 NN denotes repression
T5496 28327-28333 JJ denotes direct
T5497 28334-28349 JJ denotes transcriptional
T5498 28361-28363 IN denotes by
T5499 28364-28369 NN denotes Snail
T5500 28370-28372 CC denotes or
T5501 28373-28386 NNS denotes perturbations
T5502 28387-28389 IN denotes in
T5503 28390-28392 NN denotes AJ
T5504 28393-28402 NN denotes formation
T5505 28403-28409 VBN denotes caused
T5506 28410-28412 IN denotes by
T5507 28413-28416 DT denotes the
T5508 28417-28425 NN denotes decrease
T5509 28426-28428 IN denotes in
T5510 28429-28430 NN denotes E
T5511 28431-28439 NN denotes cadherin
T5512 28430-28431 HYPH denotes -
T5513 28445-28455 NN denotes expression
T5514 28440-28444 NN denotes gene
T5515 28455-28456 . denotes .
T5516 28456-28626 sentence denotes To distinguish between these possibilities, we tested whether α-catenin levels could be restored by exogenous expression of E-cadherin in Snail-expressing keratinocytes.
T5517 28457-28459 TO denotes To
T5518 28460-28471 VB denotes distinguish
T5519 28504-28510 VBD denotes tested
T5520 28472-28479 IN denotes between
T5521 28480-28485 DT denotes these
T5522 28486-28499 NNS denotes possibilities
T5523 28499-28501 , denotes ,
T5524 28501-28503 PRP denotes we
T5525 28511-28518 IN denotes whether
T5526 28545-28553 VBN denotes restored
T5527 28519-28520 NN denotes α
T5528 28521-28528 NN denotes catenin
T5529 28520-28521 HYPH denotes -
T5530 28529-28535 NNS denotes levels
T5531 28536-28541 MD denotes could
T5532 28542-28544 VB denotes be
T5533 28554-28556 IN denotes by
T5534 28557-28566 JJ denotes exogenous
T5535 28567-28577 NN denotes expression
T5536 28578-28580 IN denotes of
T5537 28581-28582 NN denotes E
T5538 28583-28591 NN denotes cadherin
T5539 28582-28583 HYPH denotes -
T5540 28592-28594 IN denotes in
T5541 28595-28600 NN denotes Snail
T5542 28601-28611 VBG denotes expressing
T5543 28600-28601 HYPH denotes -
T5544 28612-28625 NNS denotes keratinocytes
T5545 28625-28626 . denotes .
T5546 28626-28781 sentence denotes As shown in Figure 4C, transiently transfected keratinocytes expressing HA-tagged Snail displayed a loss of E-cadherin and α-catenin at cell-cell borders.
T5547 28627-28629 IN denotes As
T5548 28630-28635 VBN denotes shown
T5549 28715-28724 VBD denotes displayed
T5550 28636-28638 IN denotes in
T5551 28639-28645 NN denotes Figure
T5552 28646-28648 NN denotes 4C
T5553 28648-28650 , denotes ,
T5554 28650-28661 RB denotes transiently
T5555 28662-28673 VBN denotes transfected
T5556 28674-28687 NNS denotes keratinocytes
T5557 28688-28698 VBG denotes expressing
T5558 28699-28701 NN denotes HA
T5559 28702-28708 VBN denotes tagged
T5560 28701-28702 HYPH denotes -
T5561 28709-28714 NN denotes Snail
T5562 28725-28726 DT denotes a
T5563 28727-28731 NN denotes loss
T5564 28732-28734 IN denotes of
T5565 28735-28736 NN denotes E
T5566 28737-28745 NN denotes cadherin
T5567 28736-28737 HYPH denotes -
T5568 28746-28749 CC denotes and
T5569 28750-28751 NN denotes α
T5570 28752-28759 NN denotes catenin
T5571 28751-28752 HYPH denotes -
T5572 28760-28762 IN denotes at
T5573 28763-28767 NN denotes cell
T5574 28768-28772 NN denotes cell
T5575 28767-28768 HYPH denotes -
T5576 28773-28780 NNS denotes borders
T5577 28780-28781 . denotes .
T5578 28781-28971 sentence denotes Coexpression of exogenous HA-tagged E-cadherin not only enabled cell-cell border localization of E-cadherin protein, but also rescued the cell-cell border staining of α-catenin (Figure 4C).
T5579 28782-28794 NN denotes Coexpression
T5580 28838-28845 VBD denotes enabled
T5581 28795-28797 IN denotes of
T5582 28798-28807 JJ denotes exogenous
T5583 28820-28828 NN denotes cadherin
T5584 28808-28810 NN denotes HA
T5585 28811-28817 VBN denotes tagged
T5586 28810-28811 HYPH denotes -
T5587 28818-28819 NN denotes E
T5588 28819-28820 HYPH denotes -
T5589 28829-28832 RB denotes not
T5590 28833-28837 RB denotes only
T5591 28846-28850 NN denotes cell
T5592 28851-28855 NN denotes cell
T5593 28850-28851 HYPH denotes -
T5594 28863-28875 NN denotes localization
T5595 28856-28862 NN denotes border
T5596 28876-28878 IN denotes of
T5597 28879-28880 NN denotes E
T5598 28881-28889 NN denotes cadherin
T5599 28880-28881 HYPH denotes -
T5600 28890-28897 NN denotes protein
T5601 28897-28899 , denotes ,
T5602 28899-28902 CC denotes but
T5603 28903-28907 RB denotes also
T5604 28908-28915 VBD denotes rescued
T5605 28916-28919 DT denotes the
T5606 28937-28945 NN denotes staining
T5607 28920-28924 NN denotes cell
T5608 28925-28929 NN denotes cell
T5609 28924-28925 HYPH denotes -
T5610 28930-28936 NN denotes border
T5611 28946-28948 IN denotes of
T5612 28949-28950 NN denotes α
T5613 28951-28958 NN denotes catenin
T5614 28950-28951 HYPH denotes -
T5615 28959-28960 -LRB- denotes (
T5616 28967-28969 NN denotes 4C
T5617 28960-28966 NN denotes Figure
T5618 28969-28970 -RRB- denotes )
T5619 28970-28971 . denotes .
T5620 28971-29131 sentence denotes The ability to restore α-catenin expression and localization under these conditions argues against the notion that Snail transcriptionally represses α-catenin.
T5621 28972-28975 DT denotes The
T5622 28976-28983 NN denotes ability
T5623 29056-29062 VBZ denotes argues
T5624 28984-28986 TO denotes to
T5625 28987-28994 VB denotes restore
T5626 28995-28996 NN denotes α
T5627 28997-29004 NN denotes catenin
T5628 28996-28997 HYPH denotes -
T5629 29005-29015 NN denotes expression
T5630 29016-29019 CC denotes and
T5631 29020-29032 NN denotes localization
T5632 29033-29038 IN denotes under
T5633 29039-29044 DT denotes these
T5634 29045-29055 NNS denotes conditions
T5635 29063-29070 IN denotes against
T5636 29071-29074 DT denotes the
T5637 29075-29081 NN denotes notion
T5638 29082-29086 IN denotes that
T5639 29111-29120 VBZ denotes represses
T5640 29087-29092 NN denotes Snail
T5641 29093-29110 RB denotes transcriptionally
T5642 29121-29122 NN denotes α
T5643 29123-29130 NN denotes catenin
T5644 29122-29123 HYPH denotes -
T5645 29130-29131 . denotes .
T5646 29131-29262 sentence denotes Rather, the findings are consistent with a previous report that E-cadherin is required for the translation of α-catenin mRNA [22].
T5647 29132-29138 RB denotes Rather
T5648 29153-29156 VBP denotes are
T5649 29138-29140 , denotes ,
T5650 29140-29143 DT denotes the
T5651 29144-29152 NNS denotes findings
T5652 29157-29167 JJ denotes consistent
T5653 29168-29172 IN denotes with
T5654 29173-29174 DT denotes a
T5655 29184-29190 NN denotes report
T5656 29175-29183 JJ denotes previous
T5657 29191-29195 IN denotes that
T5658 29210-29218 VBN denotes required
T5659 29196-29197 NN denotes E
T5660 29198-29206 NN denotes cadherin
T5661 29197-29198 HYPH denotes -
T5662 29207-29209 VBZ denotes is
T5663 29219-29222 IN denotes for
T5664 29223-29226 DT denotes the
T5665 29227-29238 NN denotes translation
T5666 29239-29241 IN denotes of
T5667 29242-29243 NN denotes α
T5668 29244-29251 NN denotes catenin
T5669 29243-29244 HYPH denotes -
T5670 29252-29256 NN denotes mRNA
T5671 29257-29258 -LRB- denotes [
T5672 29258-29260 CD denotes 22
T5673 29260-29261 -RRB- denotes ]
T5674 29261-29262 . denotes .
T5675 29262-29453 sentence denotes Despite the reductions in AJ markers, Tg skin still displayed sealed membranes and intercellular junctions that were largely intact, as judged by ultrastructural analyses (unpublished data).
T5676 29263-29270 IN denotes Despite
T5677 29315-29324 VBD denotes displayed
T5678 29271-29274 DT denotes the
T5679 29275-29285 NNS denotes reductions
T5680 29286-29288 IN denotes in
T5681 29289-29291 NN denotes AJ
T5682 29292-29299 NNS denotes markers
T5683 29299-29301 , denotes ,
T5684 29301-29303 NN denotes Tg
T5685 29304-29308 NN denotes skin
T5686 29309-29314 RB denotes still
T5687 29325-29331 JJ denotes sealed
T5688 29332-29341 NNS denotes membranes
T5689 29342-29345 CC denotes and
T5690 29346-29359 JJ denotes intercellular
T5691 29360-29369 NNS denotes junctions
T5692 29370-29374 WDT denotes that
T5693 29375-29379 VBD denotes were
T5694 29380-29387 RB denotes largely
T5695 29388-29394 JJ denotes intact
T5696 29394-29396 , denotes ,
T5697 29396-29398 IN denotes as
T5698 29399-29405 VBN denotes judged
T5699 29406-29408 IN denotes by
T5700 29409-29424 JJ denotes ultrastructural
T5701 29425-29433 NNS denotes analyses
T5702 29434-29435 -LRB- denotes (
T5703 29447-29451 NNS denotes data
T5704 29435-29446 JJ denotes unpublished
T5705 29451-29452 -RRB- denotes )
T5706 29452-29453 . denotes .
T5707 29453-29651 sentence denotes In this respect, the skin epithelium resembled that of the hair bud, where the down-regulation in junction proteins is permissive for cell-cell remodeling without abrogating intercellular adhesion.
T5708 29454-29456 IN denotes In
T5709 29491-29500 VBD denotes resembled
T5710 29457-29461 DT denotes this
T5711 29462-29469 NN denotes respect
T5712 29469-29471 , denotes ,
T5713 29471-29474 DT denotes the
T5714 29480-29490 NN denotes epithelium
T5715 29475-29479 NN denotes skin
T5716 29501-29505 DT denotes that
T5717 29506-29508 IN denotes of
T5718 29509-29512 DT denotes the
T5719 29518-29521 NN denotes bud
T5720 29513-29517 NN denotes hair
T5721 29521-29523 , denotes ,
T5722 29523-29528 WRB denotes where
T5723 29570-29572 VBZ denotes is
T5724 29529-29532 DT denotes the
T5725 29538-29548 NN denotes regulation
T5726 29533-29537 JJ denotes down
T5727 29537-29538 HYPH denotes -
T5728 29549-29551 IN denotes in
T5729 29552-29560 NN denotes junction
T5730 29561-29569 NN denotes proteins
T5731 29573-29583 JJ denotes permissive
T5732 29584-29587 IN denotes for
T5733 29588-29592 NN denotes cell
T5734 29593-29597 NN denotes cell
T5735 29592-29593 HYPH denotes -
T5736 29598-29608 NN denotes remodeling
T5737 29609-29616 IN denotes without
T5738 29617-29627 VBG denotes abrogating
T5739 29628-29641 JJ denotes intercellular
T5740 29642-29650 NN denotes adhesion
T5741 29650-29651 . denotes .
T5742 29651-29851 sentence denotes The similarities between Snail Tg epidermis and hair buds extended to the hyperproliferative state, leading us to wonder whether the down-regulation of AJ proteins might contribute to this condition.
T5743 29652-29655 DT denotes The
T5744 29656-29668 NNS denotes similarities
T5745 29710-29718 VBD denotes extended
T5746 29669-29676 IN denotes between
T5747 29677-29682 NN denotes Snail
T5748 29686-29695 NN denotes epidermis
T5749 29683-29685 NN denotes Tg
T5750 29696-29699 CC denotes and
T5751 29700-29704 NN denotes hair
T5752 29705-29709 NNS denotes buds
T5753 29719-29721 IN denotes to
T5754 29722-29725 DT denotes the
T5755 29745-29750 NN denotes state
T5756 29726-29744 JJ denotes hyperproliferative
T5757 29750-29752 , denotes ,
T5758 29752-29759 VBG denotes leading
T5759 29760-29762 PRP denotes us
T5760 29763-29765 TO denotes to
T5761 29766-29772 VB denotes wonder
T5762 29773-29780 IN denotes whether
T5763 29822-29832 VB denotes contribute
T5764 29781-29784 DT denotes the
T5765 29790-29800 NN denotes regulation
T5766 29785-29789 JJ denotes down
T5767 29789-29790 HYPH denotes -
T5768 29801-29803 IN denotes of
T5769 29804-29806 NN denotes AJ
T5770 29807-29815 NN denotes proteins
T5771 29816-29821 MD denotes might
T5772 29833-29835 IN denotes to
T5773 29836-29840 DT denotes this
T5774 29841-29850 NN denotes condition
T5775 29850-29851 . denotes .
T5776 29851-30061 sentence denotes Given the increase in pMAPK staining in Snail Tg epidermis (see Figure 2G), we used pMAPK levels as our assay to test whether the loss of E-cadherin contributed to the Snail-mediated increase in proliferation.
T5777 29852-29857 VBN denotes Given
T5778 29931-29935 VBD denotes used
T5779 29858-29861 DT denotes the
T5780 29862-29870 NN denotes increase
T5781 29871-29873 IN denotes in
T5782 29874-29879 NN denotes pMAPK
T5783 29880-29888 NN denotes staining
T5784 29889-29891 IN denotes in
T5785 29892-29897 NN denotes Snail
T5786 29901-29910 NN denotes epidermis
T5787 29898-29900 NN denotes Tg
T5788 29911-29912 -LRB- denotes (
T5789 29912-29915 VB denotes see
T5790 29916-29922 NN denotes Figure
T5791 29923-29925 NN denotes 2G
T5792 29925-29926 -RRB- denotes )
T5793 29926-29928 , denotes ,
T5794 29928-29930 PRP denotes we
T5795 29936-29941 NN denotes pMAPK
T5796 29942-29948 NNS denotes levels
T5797 29949-29951 IN denotes as
T5798 29952-29955 PRP$ denotes our
T5799 29956-29961 NN denotes assay
T5800 29962-29964 TO denotes to
T5801 29965-29969 VB denotes test
T5802 29970-29977 IN denotes whether
T5803 30001-30012 VBD denotes contributed
T5804 29978-29981 DT denotes the
T5805 29982-29986 NN denotes loss
T5806 29987-29989 IN denotes of
T5807 29990-29991 NN denotes E
T5808 29992-30000 NN denotes cadherin
T5809 29991-29992 HYPH denotes -
T5810 30013-30015 IN denotes to
T5811 30016-30019 DT denotes the
T5812 30035-30043 NN denotes increase
T5813 30020-30025 NN denotes Snail
T5814 30026-30034 VBN denotes mediated
T5815 30025-30026 HYPH denotes -
T5816 30044-30046 IN denotes in
T5817 30047-30060 NN denotes proliferation
T5818 30060-30061 . denotes .
T5819 30061-30234 sentence denotes Consistent with our in vivo observations, transfected keratinocytes expressing Snail exhibited a substantial increase in pMAPK levels relative to control cells (Figure 4D).
T5820 30062-30072 JJ denotes Consistent
T5821 30147-30156 VBD denotes exhibited
T5822 30073-30077 IN denotes with
T5823 30078-30081 PRP$ denotes our
T5824 30090-30102 NNS denotes observations
T5825 30082-30084 FW denotes in
T5826 30085-30089 FW denotes vivo
T5827 30102-30104 , denotes ,
T5828 30104-30115 VBN denotes transfected
T5829 30116-30129 NNS denotes keratinocytes
T5830 30130-30140 VBG denotes expressing
T5831 30141-30146 NN denotes Snail
T5832 30157-30158 DT denotes a
T5833 30171-30179 NN denotes increase
T5834 30159-30170 JJ denotes substantial
T5835 30180-30182 IN denotes in
T5836 30183-30188 NN denotes pMAPK
T5837 30189-30195 NNS denotes levels
T5838 30196-30204 JJ denotes relative
T5839 30205-30207 IN denotes to
T5840 30208-30215 NN denotes control
T5841 30216-30221 NNS denotes cells
T5842 30222-30223 -LRB- denotes (
T5843 30230-30232 NN denotes 4D
T5844 30223-30229 NN denotes Figure
T5845 30232-30233 -RRB- denotes )
T5846 30233-30234 . denotes .
T5847 30234-30306 sentence denotes Coexpression of E-cadherin with Snail appeared to abrogate this effect.
T5848 30235-30247 NN denotes Coexpression
T5849 30273-30281 VBD denotes appeared
T5850 30248-30250 IN denotes of
T5851 30251-30252 NN denotes E
T5852 30253-30261 NN denotes cadherin
T5853 30252-30253 HYPH denotes -
T5854 30262-30266 IN denotes with
T5855 30267-30272 NN denotes Snail
T5856 30282-30284 TO denotes to
T5857 30285-30293 VB denotes abrogate
T5858 30294-30298 DT denotes this
T5859 30299-30305 NN denotes effect
T5860 30305-30306 . denotes .
T5861 30306-30517 sentence denotes Together, these findings raised the possibility that an AJ-associated protein that is normally sequestered at the plasma membrane may participate in a proliferation signaling pathway when AJs are deconstructed.
T5862 30307-30315 RB denotes Together
T5863 30332-30338 VBD denotes raised
T5864 30315-30317 , denotes ,
T5865 30317-30322 DT denotes these
T5866 30323-30331 NNS denotes findings
T5867 30339-30342 DT denotes the
T5868 30343-30354 NN denotes possibility
T5869 30355-30359 IN denotes that
T5870 30441-30452 VB denotes participate
T5871 30360-30362 DT denotes an
T5872 30377-30384 NN denotes protein
T5873 30363-30365 NN denotes AJ
T5874 30366-30376 VBN denotes associated
T5875 30365-30366 HYPH denotes -
T5876 30385-30389 WDT denotes that
T5877 30402-30413 VBN denotes sequestered
T5878 30390-30392 VBZ denotes is
T5879 30393-30401 RB denotes normally
T5880 30414-30416 IN denotes at
T5881 30417-30420 DT denotes the
T5882 30428-30436 NN denotes membrane
T5883 30421-30427 NN denotes plasma
T5884 30437-30440 MD denotes may
T5885 30453-30455 IN denotes in
T5886 30456-30457 DT denotes a
T5887 30482-30489 NN denotes pathway
T5888 30458-30471 NN denotes proliferation
T5889 30472-30481 NN denotes signaling
T5890 30490-30494 WRB denotes when
T5891 30503-30516 VBN denotes deconstructed
T5892 30495-30498 NNS denotes AJs
T5893 30499-30502 VBP denotes are
T5894 30516-30517 . denotes .
T5895 30517-30757 sentence denotes Numerous studies have correlated a down-regulation of E-cadherin with a translocation of β-catenin to the nucleus and a transactivation of genes that are regulated by the LEF-1/T cell factor (TCF) family of DNA binding proteins [23,24,25].
T5896 30518-30526 JJ denotes Numerous
T5897 30527-30534 NNS denotes studies
T5898 30540-30550 VBN denotes correlated
T5899 30535-30539 VBP denotes have
T5900 30551-30552 DT denotes a
T5901 30558-30568 NN denotes regulation
T5902 30553-30557 JJ denotes down
T5903 30557-30558 HYPH denotes -
T5904 30569-30571 IN denotes of
T5905 30572-30573 NN denotes E
T5906 30574-30582 NN denotes cadherin
T5907 30573-30574 HYPH denotes -
T5908 30583-30587 IN denotes with
T5909 30588-30589 DT denotes a
T5910 30590-30603 NN denotes translocation
T5911 30604-30606 IN denotes of
T5912 30607-30608 NN denotes β
T5913 30609-30616 NN denotes catenin
T5914 30608-30609 HYPH denotes -
T5915 30617-30619 IN denotes to
T5916 30620-30623 DT denotes the
T5917 30624-30631 NN denotes nucleus
T5918 30632-30635 CC denotes and
T5919 30636-30637 DT denotes a
T5920 30638-30653 NN denotes transactivation
T5921 30654-30656 IN denotes of
T5922 30657-30662 NNS denotes genes
T5923 30663-30667 WDT denotes that
T5924 30672-30681 VBN denotes regulated
T5925 30668-30671 VBP denotes are
T5926 30682-30684 IN denotes by
T5927 30685-30688 DT denotes the
T5928 30715-30721 NN denotes family
T5929 30689-30692 NN denotes LEF
T5930 30692-30693 HYPH denotes -
T5931 30693-30694 CD denotes 1
T5932 30694-30695 HYPH denotes /
T5933 30695-30696 NN denotes T
T5934 30697-30701 NN denotes cell
T5935 30702-30708 NN denotes factor
T5936 30709-30710 -LRB- denotes (
T5937 30710-30713 NN denotes TCF
T5938 30713-30714 -RRB- denotes )
T5939 30722-30724 IN denotes of
T5940 30725-30728 NN denotes DNA
T5941 30729-30736 VBG denotes binding
T5942 30737-30745 NN denotes proteins
T5943 30746-30747 -LRB- denotes [
T5944 30753-30755 CD denotes 25
T5945 30747-30749 CD denotes 23
T5946 30749-30750 , denotes ,
T5947 30750-30752 CD denotes 24
T5948 30752-30753 , denotes ,
T5949 30755-30756 -RRB- denotes ]
T5950 30756-30757 . denotes .
T5951 30757-30963 sentence denotes The presence of nuclear cyclin D in hyperproliferative Snail Tg epidermis was particularly intriguing since prior studies have reported cyclin D gene as a direct target of TCF/β-catenin transcription [26].
T5952 30758-30761 DT denotes The
T5953 30762-30770 NN denotes presence
T5954 30832-30835 VBD denotes was
T5955 30771-30773 IN denotes of
T5956 30774-30781 JJ denotes nuclear
T5957 30789-30790 NN denotes D
T5958 30782-30788 NN denotes cyclin
T5959 30791-30793 IN denotes in
T5960 30794-30812 JJ denotes hyperproliferative
T5961 30822-30831 NN denotes epidermis
T5962 30813-30818 NN denotes Snail
T5963 30819-30821 NN denotes Tg
T5964 30836-30848 RB denotes particularly
T5965 30849-30859 JJ denotes intriguing
T5966 30860-30865 IN denotes since
T5967 30885-30893 VBN denotes reported
T5968 30866-30871 JJ denotes prior
T5969 30872-30879 NNS denotes studies
T5970 30880-30884 VBP denotes have
T5971 30894-30900 NN denotes cyclin
T5972 30901-30902 NN denotes D
T5973 30903-30907 NN denotes gene
T5974 30908-30910 IN denotes as
T5975 30911-30912 DT denotes a
T5976 30920-30926 NN denotes target
T5977 30913-30919 JJ denotes direct
T5978 30927-30929 IN denotes of
T5979 30930-30933 NN denotes TCF
T5980 30934-30935 NN denotes β
T5981 30933-30934 HYPH denotes /
T5982 30944-30957 NN denotes transcription
T5983 30935-30936 HYPH denotes -
T5984 30936-30943 NN denotes catenin
T5985 30958-30959 -LRB- denotes [
T5986 30959-30961 CD denotes 26
T5987 30961-30962 -RRB- denotes ]
T5988 30962-30963 . denotes .
T5989 30963-31173 sentence denotes This said, we did not detect nuclear β-catenin in our Tg epidermis, and mating the Snail Tg mice against the TOPGal reporter mouse [20] gave no signs of ectopic LEF-1/Tcf/β-catenin activity (unpublished data).
T5990 30964-30968 DT denotes This
T5991 30969-30973 VBN denotes said
T5992 30986-30992 VB denotes detect
T5993 30973-30975 , denotes ,
T5994 30975-30977 PRP denotes we
T5995 30978-30981 VBD denotes did
T5996 30982-30985 RB denotes not
T5997 30993-31000 JJ denotes nuclear
T5998 31003-31010 NN denotes catenin
T5999 31001-31002 NN denotes β
T6000 31002-31003 HYPH denotes -
T6001 31011-31013 IN denotes in
T6002 31014-31017 PRP$ denotes our
T6003 31021-31030 NN denotes epidermis
T6004 31018-31020 NN denotes Tg
T6005 31030-31032 , denotes ,
T6006 31032-31035 CC denotes and
T6007 31036-31042 VBG denotes mating
T6008 31100-31104 VBD denotes gave
T6009 31043-31046 DT denotes the
T6010 31056-31060 NNS denotes mice
T6011 31047-31052 NN denotes Snail
T6012 31053-31055 NN denotes Tg
T6013 31061-31068 IN denotes against
T6014 31069-31072 DT denotes the
T6015 31089-31094 NN denotes mouse
T6016 31073-31079 NN denotes TOPGal
T6017 31080-31088 NN denotes reporter
T6018 31095-31096 -LRB- denotes [
T6019 31096-31098 CD denotes 20
T6020 31098-31099 -RRB- denotes ]
T6021 31105-31107 DT denotes no
T6022 31108-31113 NNS denotes signs
T6023 31114-31116 IN denotes of
T6024 31117-31124 JJ denotes ectopic
T6025 31145-31153 NN denotes activity
T6026 31125-31128 NN denotes LEF
T6027 31128-31129 HYPH denotes -
T6028 31129-31130 CD denotes 1
T6029 31130-31131 HYPH denotes /
T6030 31131-31134 NN denotes Tcf
T6031 31134-31135 HYPH denotes /
T6032 31135-31136 NN denotes β
T6033 31137-31144 NN denotes catenin
T6034 31136-31137 HYPH denotes -
T6035 31154-31155 -LRB- denotes (
T6036 31167-31171 NNS denotes data
T6037 31155-31166 JJ denotes unpublished
T6038 31171-31172 -RRB- denotes )
T6039 31172-31173 . denotes .
T6040 31173-31314 sentence denotes We next turned to the presence of cytoplasmic Ajuba for a possible mechanistic link to the proliferative increase in our Snail Tg epidermis.
T6041 31174-31176 PRP denotes We
T6042 31182-31188 VBD denotes turned
T6043 31177-31181 RB denotes next
T6044 31189-31191 IN denotes to
T6045 31192-31195 DT denotes the
T6046 31196-31204 NN denotes presence
T6047 31205-31207 IN denotes of
T6048 31208-31219 JJ denotes cytoplasmic
T6049 31220-31225 NN denotes Ajuba
T6050 31226-31229 IN denotes for
T6051 31230-31231 DT denotes a
T6052 31253-31257 NN denotes link
T6053 31232-31240 JJ denotes possible
T6054 31241-31252 JJ denotes mechanistic
T6055 31258-31260 IN denotes to
T6056 31261-31264 DT denotes the
T6057 31279-31287 NN denotes increase
T6058 31265-31278 JJ denotes proliferative
T6059 31288-31290 IN denotes in
T6060 31291-31294 PRP$ denotes our
T6061 31304-31313 NN denotes epidermis
T6062 31295-31300 NN denotes Snail
T6063 31301-31303 NN denotes Tg
T6064 31313-31314 . denotes .
T6065 31314-31564 sentence denotes In addition to its documented ability to bind α-catenin [10], Ajuba can also associate with growth factor receptor-bound protein-2 (Grb-2)/son of sevenless (Sos), the nucleotide exchange factor for Ras, which is upstream from activation of MAPK [9].
T6066 31315-31317 IN denotes In
T6067 31392-31401 VB denotes associate
T6068 31318-31326 NN denotes addition
T6069 31327-31329 IN denotes to
T6070 31330-31333 PRP$ denotes its
T6071 31345-31352 NN denotes ability
T6072 31334-31344 VBN denotes documented
T6073 31353-31355 TO denotes to
T6074 31356-31360 VB denotes bind
T6075 31361-31362 NN denotes α
T6076 31363-31370 NN denotes catenin
T6077 31362-31363 HYPH denotes -
T6078 31371-31372 -LRB- denotes [
T6079 31372-31374 CD denotes 10
T6080 31374-31375 -RRB- denotes ]
T6081 31375-31377 , denotes ,
T6082 31377-31382 NN denotes Ajuba
T6083 31383-31386 MD denotes can
T6084 31387-31391 RB denotes also
T6085 31402-31406 IN denotes with
T6086 31407-31413 NN denotes growth
T6087 31414-31420 NN denotes factor
T6088 31421-31429 NN denotes receptor
T6089 31430-31435 JJ denotes bound
T6090 31429-31430 HYPH denotes -
T6091 31436-31443 NN denotes protein
T6092 31443-31444 HYPH denotes -
T6093 31444-31445 CD denotes 2
T6094 31446-31447 -LRB- denotes (
T6095 31447-31450 NN denotes Grb
T6096 31450-31451 HYPH denotes -
T6097 31451-31452 CD denotes 2
T6098 31452-31453 -RRB- denotes )
T6099 31453-31454 HYPH denotes /
T6100 31454-31457 NN denotes son
T6101 31458-31460 IN denotes of
T6102 31461-31470 NN denotes sevenless
T6103 31471-31472 -LRB- denotes (
T6104 31472-31475 NN denotes Sos
T6105 31475-31476 -RRB- denotes )
T6106 31476-31478 , denotes ,
T6107 31478-31481 DT denotes the
T6108 31502-31508 NN denotes factor
T6109 31482-31492 NN denotes nucleotide
T6110 31493-31501 NN denotes exchange
T6111 31509-31512 IN denotes for
T6112 31513-31516 NN denotes Ras
T6113 31516-31518 , denotes ,
T6114 31518-31523 WDT denotes which
T6115 31524-31526 VBZ denotes is
T6116 31527-31535 RB denotes upstream
T6117 31536-31540 IN denotes from
T6118 31541-31551 NN denotes activation
T6119 31552-31554 IN denotes of
T6120 31555-31559 NN denotes MAPK
T6121 31560-31561 -LRB- denotes [
T6122 31561-31562 CD denotes 9
T6123 31562-31563 -RRB- denotes ]
T6124 31563-31564 . denotes .
T6125 31564-31722 sentence denotes Given the increase in pMAPK staining in Tg skin, we examined the possibility that Ajuba might have changed its binding partner in Snail-expressing epidermis.
T6126 31565-31570 VBN denotes Given
T6127 31617-31625 VBD denotes examined
T6128 31571-31574 DT denotes the
T6129 31575-31583 NN denotes increase
T6130 31584-31586 IN denotes in
T6131 31587-31592 NN denotes pMAPK
T6132 31593-31601 NN denotes staining
T6133 31602-31604 IN denotes in
T6134 31605-31607 NN denotes Tg
T6135 31608-31612 NN denotes skin
T6136 31612-31614 , denotes ,
T6137 31614-31616 PRP denotes we
T6138 31626-31629 DT denotes the
T6139 31630-31641 NN denotes possibility
T6140 31642-31646 IN denotes that
T6141 31664-31671 VBN denotes changed
T6142 31647-31652 NN denotes Ajuba
T6143 31653-31658 MD denotes might
T6144 31659-31663 VB denotes have
T6145 31672-31675 PRP$ denotes its
T6146 31684-31691 NN denotes partner
T6147 31676-31683 NN denotes binding
T6148 31692-31694 IN denotes in
T6149 31695-31700 NN denotes Snail
T6150 31701-31711 VBG denotes expressing
T6151 31700-31701 HYPH denotes -
T6152 31712-31721 NN denotes epidermis
T6153 31721-31722 . denotes .
T6154 31722-31911 sentence denotes Interestingly, Ajuba was readily detected in anti-Grb-2 immunoprecipitates of protein lysates from skins of Snail Tg mice but not from the corresponding wild-type (WT) animals (Figure 4E).
T6155 31723-31736 RB denotes Interestingly
T6156 31756-31764 VBN denotes detected
T6157 31736-31738 , denotes ,
T6158 31738-31743 NN denotes Ajuba
T6159 31744-31747 VBD denotes was
T6160 31748-31755 RB denotes readily
T6161 31765-31767 IN denotes in
T6162 31768-31776 JJ denotes anti-Grb
T6163 31779-31797 NNS denotes immunoprecipitates
T6164 31776-31777 HYPH denotes -
T6165 31777-31778 CD denotes 2
T6166 31798-31800 IN denotes of
T6167 31801-31808 NN denotes protein
T6168 31809-31816 NNS denotes lysates
T6169 31817-31821 IN denotes from
T6170 31822-31827 NNS denotes skins
T6171 31828-31830 IN denotes of
T6172 31831-31836 NN denotes Snail
T6173 31840-31844 NNS denotes mice
T6174 31837-31839 NN denotes Tg
T6175 31845-31848 CC denotes but
T6176 31849-31852 RB denotes not
T6177 31853-31857 IN denotes from
T6178 31858-31861 DT denotes the
T6179 31891-31898 NNS denotes animals
T6180 31862-31875 JJ denotes corresponding
T6181 31876-31880 JJ denotes wild
T6182 31881-31885 NN denotes type
T6183 31880-31881 HYPH denotes -
T6184 31886-31887 -LRB- denotes (
T6185 31887-31889 NN denotes WT
T6186 31889-31890 -RRB- denotes )
T6187 31899-31900 -LRB- denotes (
T6188 31907-31909 NN denotes 4E
T6189 31900-31906 NN denotes Figure
T6190 31909-31910 -RRB- denotes )
T6191 31910-31911 . denotes .
T6192 31911-32138 sentence denotes When these experiments were repeated with α-catenin-null epidermis, a similar Grb-2-Ajuba association was detected, and again, this interaction was not detected in the protein extracts from control littermate skin (Figure 4E).
T6193 31912-31916 WRB denotes When
T6194 31940-31948 VBN denotes repeated
T6195 31917-31922 DT denotes these
T6196 31923-31934 NNS denotes experiments
T6197 31935-31939 VBD denotes were
T6198 32018-32026 VBN denotes detected
T6199 31949-31953 IN denotes with
T6200 31954-31955 NN denotes α
T6201 31956-31963 NN denotes catenin
T6202 31955-31956 HYPH denotes -
T6203 31964-31968 JJ denotes null
T6204 31963-31964 HYPH denotes -
T6205 31969-31978 NN denotes epidermis
T6206 31978-31980 , denotes ,
T6207 31980-31981 DT denotes a
T6208 32002-32013 NN denotes association
T6209 31982-31989 JJ denotes similar
T6210 31990-31993 NN denotes Grb
T6211 31996-32001 NN denotes Ajuba
T6212 31993-31994 HYPH denotes -
T6213 31994-31995 CD denotes 2
T6214 31995-31996 HYPH denotes -
T6215 32014-32017 VBD denotes was
T6216 32026-32028 , denotes ,
T6217 32028-32031 CC denotes and
T6218 32032-32037 RB denotes again
T6219 32064-32072 VBN denotes detected
T6220 32037-32039 , denotes ,
T6221 32039-32043 DT denotes this
T6222 32044-32055 NN denotes interaction
T6223 32056-32059 VBD denotes was
T6224 32060-32063 RB denotes not
T6225 32073-32075 IN denotes in
T6226 32076-32079 DT denotes the
T6227 32088-32096 NNS denotes extracts
T6228 32080-32087 NN denotes protein
T6229 32097-32101 IN denotes from
T6230 32102-32109 NN denotes control
T6231 32121-32125 NN denotes skin
T6232 32110-32120 NN denotes littermate
T6233 32126-32127 -LRB- denotes (
T6234 32134-32136 NN denotes 4E
T6235 32127-32133 NN denotes Figure
T6236 32136-32137 -RRB- denotes )
T6237 32137-32138 . denotes .
T6238 32138-32346 sentence denotes Together, these data demonstrate that the reduction in α-catenin levels, either by Snail-mediated down-regulation of E-cadherin or by α-catenin conditional targeting, allows Ajuba to interact with Grb-2/Sos.
T6239 32139-32147 RB denotes Together
T6240 32160-32171 VBP denotes demonstrate
T6241 32147-32149 , denotes ,
T6242 32149-32154 DT denotes these
T6243 32155-32159 NNS denotes data
T6244 32172-32176 IN denotes that
T6245 32306-32312 VBZ denotes allows
T6246 32177-32180 DT denotes the
T6247 32181-32190 NN denotes reduction
T6248 32191-32193 IN denotes in
T6249 32194-32195 NN denotes α
T6250 32196-32203 NN denotes catenin
T6251 32195-32196 HYPH denotes -
T6252 32204-32210 NNS denotes levels
T6253 32210-32212 , denotes ,
T6254 32212-32218 CC denotes either
T6255 32219-32221 IN denotes by
T6256 32222-32227 NN denotes Snail
T6257 32228-32236 VBN denotes mediated
T6258 32227-32228 HYPH denotes -
T6259 32242-32252 NN denotes regulation
T6260 32237-32241 JJ denotes down
T6261 32241-32242 HYPH denotes -
T6262 32253-32255 IN denotes of
T6263 32256-32257 NN denotes E
T6264 32258-32266 NN denotes cadherin
T6265 32257-32258 HYPH denotes -
T6266 32267-32269 CC denotes or
T6267 32270-32272 IN denotes by
T6268 32273-32274 NN denotes α
T6269 32275-32282 NN denotes catenin
T6270 32274-32275 HYPH denotes -
T6271 32283-32294 JJ denotes conditional
T6272 32295-32304 NN denotes targeting
T6273 32304-32306 , denotes ,
T6274 32313-32318 NN denotes Ajuba
T6275 32322-32330 VB denotes interact
T6276 32319-32321 TO denotes to
T6277 32331-32335 IN denotes with
T6278 32336-32339 NN denotes Grb
T6279 32339-32340 HYPH denotes -
T6280 32340-32341 CD denotes 2
T6281 32341-32342 HYPH denotes /
T6282 32342-32345 NN denotes Sos
T6283 32345-32346 . denotes .
T6284 32346-32624 sentence denotes If the competition between Grb-2/Sos and α-catenin for Ajuba is functionally relevant to the hyperproliferative state of a keratinocyte, then overexpression of Ajuba would be expected to bypass the competition and promote activation of the Ras-MAPK pathway in WT keratinocytes.
T6285 32347-32349 IN denotes If
T6286 32408-32410 VBZ denotes is
T6287 32350-32353 DT denotes the
T6288 32354-32365 NN denotes competition
T6289 32366-32373 IN denotes between
T6290 32374-32377 NN denotes Grb
T6291 32377-32378 HYPH denotes -
T6292 32378-32379 CD denotes 2
T6293 32379-32380 HYPH denotes /
T6294 32380-32383 NN denotes Sos
T6295 32384-32387 CC denotes and
T6296 32388-32389 NN denotes α
T6297 32390-32397 NN denotes catenin
T6298 32389-32390 HYPH denotes -
T6299 32398-32401 IN denotes for
T6300 32402-32407 NN denotes Ajuba
T6301 32522-32530 VBN denotes expected
T6302 32411-32423 RB denotes functionally
T6303 32424-32432 JJ denotes relevant
T6304 32433-32435 IN denotes to
T6305 32436-32439 DT denotes the
T6306 32459-32464 NN denotes state
T6307 32440-32458 JJ denotes hyperproliferative
T6308 32465-32467 IN denotes of
T6309 32468-32469 DT denotes a
T6310 32470-32482 NN denotes keratinocyte
T6311 32482-32484 , denotes ,
T6312 32484-32488 RB denotes then
T6313 32489-32503 NN denotes overexpression
T6314 32504-32506 IN denotes of
T6315 32507-32512 NN denotes Ajuba
T6316 32513-32518 MD denotes would
T6317 32519-32521 VB denotes be
T6318 32531-32533 TO denotes to
T6319 32534-32540 VB denotes bypass
T6320 32541-32544 DT denotes the
T6321 32545-32556 NN denotes competition
T6322 32557-32560 CC denotes and
T6323 32561-32568 VB denotes promote
T6324 32569-32579 NN denotes activation
T6325 32580-32582 IN denotes of
T6326 32583-32586 DT denotes the
T6327 32596-32603 NN denotes pathway
T6328 32587-32590 NN denotes Ras
T6329 32591-32595 NN denotes MAPK
T6330 32590-32591 HYPH denotes -
T6331 32604-32606 IN denotes in
T6332 32607-32609 NN denotes WT
T6333 32610-32623 NNS denotes keratinocytes
T6334 32623-32624 . denotes .
T6335 32624-32855 sentence denotes Indeed, when serum-starved keratinocytes were transiently transfected with an Ajuba expression vector, the levels of pMAPK were not only elevated but also comparable to those transfected with the K14-HASnail transgene (Figure 4F).
T6336 32625-32631 RB denotes Indeed
T6337 32748-32752 VBD denotes were
T6338 32631-32633 , denotes ,
T6339 32633-32637 WRB denotes when
T6340 32683-32694 VBN denotes transfected
T6341 32638-32643 NN denotes serum
T6342 32644-32651 VBN denotes starved
T6343 32643-32644 HYPH denotes -
T6344 32652-32665 NNS denotes keratinocytes
T6345 32666-32670 VBD denotes were
T6346 32671-32682 RB denotes transiently
T6347 32695-32699 IN denotes with
T6348 32700-32702 DT denotes an
T6349 32720-32726 NN denotes vector
T6350 32703-32708 NN denotes Ajuba
T6351 32709-32719 NN denotes expression
T6352 32726-32728 , denotes ,
T6353 32728-32731 DT denotes the
T6354 32732-32738 NNS denotes levels
T6355 32739-32741 IN denotes of
T6356 32742-32747 NN denotes pMAPK
T6357 32753-32756 RB denotes not
T6358 32762-32770 JJ denotes elevated
T6359 32757-32761 RB denotes only
T6360 32771-32774 CC denotes but
T6361 32775-32779 RB denotes also
T6362 32780-32790 JJ denotes comparable
T6363 32791-32793 IN denotes to
T6364 32794-32799 DT denotes those
T6365 32800-32811 VBN denotes transfected
T6366 32812-32816 IN denotes with
T6367 32817-32820 DT denotes the
T6368 32833-32842 NN denotes transgene
T6369 32821-32824 NN denotes K14
T6370 32825-32832 NN denotes HASnail
T6371 32824-32825 HYPH denotes -
T6372 32843-32844 -LRB- denotes (
T6373 32851-32853 NN denotes 4F
T6374 32844-32850 NN denotes Figure
T6375 32853-32854 -RRB- denotes )
T6376 32854-32855 . denotes .
T6377 32855-33037 sentence denotes This activation was abolished when cells were treated with a small peptide inhibitor that specifically interrupts the Grb-2/Sos interaction (Figure 4F; see lanes marked “inh”) [27].
T6378 32856-32860 DT denotes This
T6379 32861-32871 NN denotes activation
T6380 32876-32885 VBN denotes abolished
T6381 32872-32875 VBD denotes was
T6382 32886-32890 WRB denotes when
T6383 32902-32909 VBN denotes treated
T6384 32891-32896 NNS denotes cells
T6385 32897-32901 VBD denotes were
T6386 32910-32914 IN denotes with
T6387 32915-32916 DT denotes a
T6388 32931-32940 NN denotes inhibitor
T6389 32917-32922 JJ denotes small
T6390 32923-32930 NN denotes peptide
T6391 32941-32945 WDT denotes that
T6392 32959-32969 VBZ denotes interrupts
T6393 32946-32958 RB denotes specifically
T6394 32970-32973 DT denotes the
T6395 32984-32995 NN denotes interaction
T6396 32974-32977 NN denotes Grb
T6397 32977-32978 HYPH denotes -
T6398 32978-32979 CD denotes 2
T6399 32979-32980 HYPH denotes /
T6400 32980-32983 NN denotes Sos
T6401 32996-32997 -LRB- denotes (
T6402 33004-33006 NN denotes 4F
T6403 32997-33003 NN denotes Figure
T6404 33006-33007 : denotes ;
T6405 33008-33011 VB denotes see
T6406 33012-33017 NNS denotes lanes
T6407 33018-33024 VBN denotes marked
T6408 33025-33026 `` denotes “
T6409 33026-33029 NN denotes inh
T6410 33029-33030 '' denotes ”
T6411 33030-33031 -RRB- denotes )
T6412 33032-33033 -LRB- denotes [
T6413 33033-33035 CD denotes 27
T6414 33035-33036 -RRB- denotes ]
T6415 33036-33037 . denotes .
T6416 33037-33131 sentence denotes Ajuba's pre-LIM domain is the segment that associates with Grb-2's Src-homology 3 domain [9].
T6417 33038-33043 NN denotes Ajuba
T6418 33054-33060 NN denotes domain
T6419 33043-33045 POS denotes 's
T6420 33046-33053 JJ denotes pre-LIM
T6421 33061-33063 VBZ denotes is
T6422 33064-33067 DT denotes the
T6423 33068-33075 NN denotes segment
T6424 33076-33080 WDT denotes that
T6425 33081-33091 VBZ denotes associates
T6426 33092-33096 IN denotes with
T6427 33097-33100 NN denotes Grb
T6428 33120-33126 NN denotes domain
T6429 33100-33101 HYPH denotes -
T6430 33101-33102 CD denotes 2
T6431 33102-33104 POS denotes 's
T6432 33105-33108 NN denotes Src
T6433 33109-33117 NN denotes homology
T6434 33108-33109 HYPH denotes -
T6435 33118-33119 CD denotes 3
T6436 33127-33128 -LRB- denotes [
T6437 33128-33129 CD denotes 9
T6438 33129-33130 -RRB- denotes ]
T6439 33130-33131 . denotes .
T6440 33131-33256 sentence denotes When this domain was overexpressed in serum-starved keratinocytes, a comparable elevation in pMAPK was observed (Figure 4F).
T6441 33132-33136 WRB denotes When
T6442 33153-33166 VBN denotes overexpressed
T6443 33137-33141 DT denotes this
T6444 33142-33148 NN denotes domain
T6445 33149-33152 VBD denotes was
T6446 33235-33243 VBN denotes observed
T6447 33167-33169 IN denotes in
T6448 33170-33175 NN denotes serum
T6449 33176-33183 VBN denotes starved
T6450 33175-33176 HYPH denotes -
T6451 33184-33197 NNS denotes keratinocytes
T6452 33197-33199 , denotes ,
T6453 33199-33200 DT denotes a
T6454 33212-33221 NN denotes elevation
T6455 33201-33211 JJ denotes comparable
T6456 33222-33224 IN denotes in
T6457 33225-33230 NN denotes pMAPK
T6458 33231-33234 VBD denotes was
T6459 33244-33245 -LRB- denotes (
T6460 33252-33254 NN denotes 4F
T6461 33245-33251 NN denotes Figure
T6462 33254-33255 -RRB- denotes )
T6463 33255-33256 . denotes .
T6464 33256-33360 sentence denotes As expected, the small peptide inhibitor that interrupts the Grb-2/Sos association blocked the effects.
T6465 33257-33259 IN denotes As
T6466 33260-33268 VBN denotes expected
T6467 33340-33347 VBD denotes blocked
T6468 33268-33270 , denotes ,
T6469 33270-33273 DT denotes the
T6470 33288-33297 NN denotes inhibitor
T6471 33274-33279 JJ denotes small
T6472 33280-33287 NN denotes peptide
T6473 33298-33302 WDT denotes that
T6474 33303-33313 VBZ denotes interrupts
T6475 33314-33317 DT denotes the
T6476 33328-33339 NN denotes association
T6477 33318-33321 NN denotes Grb
T6478 33321-33322 HYPH denotes -
T6479 33322-33323 CD denotes 2
T6480 33323-33324 HYPH denotes /
T6481 33324-33327 NN denotes Sos
T6482 33348-33351 DT denotes the
T6483 33352-33359 NNS denotes effects
T6484 33359-33360 . denotes .
T6485 33360-33585 sentence denotes These data suggested that by elevating cytosolic Ajuba levels, Ajuba's pre-LIM domain may associate with Grb-2/Sos in a manner that stimulates its nucleotide exchange activity and leads to activation of the Ras-MAPK pathway.
T6486 33361-33366 DT denotes These
T6487 33367-33371 NNS denotes data
T6488 33372-33381 VBD denotes suggested
T6489 33382-33386 IN denotes that
T6490 33451-33460 VB denotes associate
T6491 33387-33389 IN denotes by
T6492 33390-33399 VBG denotes elevating
T6493 33400-33409 JJ denotes cytosolic
T6494 33416-33422 NNS denotes levels
T6495 33410-33415 NN denotes Ajuba
T6496 33422-33424 , denotes ,
T6497 33424-33429 NN denotes Ajuba
T6498 33440-33446 NN denotes domain
T6499 33429-33431 POS denotes 's
T6500 33432-33439 JJ denotes pre-LIM
T6501 33447-33450 MD denotes may
T6502 33461-33465 IN denotes with
T6503 33466-33469 NN denotes Grb
T6504 33469-33470 HYPH denotes -
T6505 33470-33471 CD denotes 2
T6506 33471-33472 HYPH denotes /
T6507 33472-33475 NN denotes Sos
T6508 33476-33478 IN denotes in
T6509 33479-33480 DT denotes a
T6510 33481-33487 NN denotes manner
T6511 33488-33492 WDT denotes that
T6512 33493-33503 VBZ denotes stimulates
T6513 33504-33507 PRP$ denotes its
T6514 33528-33536 NN denotes activity
T6515 33508-33518 NN denotes nucleotide
T6516 33519-33527 NN denotes exchange
T6517 33537-33540 CC denotes and
T6518 33541-33546 VBZ denotes leads
T6519 33547-33549 IN denotes to
T6520 33550-33560 NN denotes activation
T6521 33561-33563 IN denotes of
T6522 33564-33567 DT denotes the
T6523 33577-33584 NN denotes pathway
T6524 33568-33571 NN denotes Ras
T6525 33572-33576 NN denotes MAPK
T6526 33571-33572 HYPH denotes -
T6527 33584-33585 . denotes .
T6528 33585-33795 sentence denotes Although this pathway provides one mechanism by which Snail expression and proliferation may be coupled in skin epithelium, proliferative circuitries involving AJs are known to be complex and often interwoven.
T6529 33586-33594 IN denotes Although
T6530 33608-33616 VBZ denotes provides
T6531 33595-33599 DT denotes this
T6532 33600-33607 NN denotes pathway
T6533 33754-33759 VBN denotes known
T6534 33617-33620 CD denotes one
T6535 33621-33630 NN denotes mechanism
T6536 33631-33633 IN denotes by
T6537 33682-33689 VBN denotes coupled
T6538 33634-33639 WDT denotes which
T6539 33640-33645 NN denotes Snail
T6540 33646-33656 NN denotes expression
T6541 33657-33660 CC denotes and
T6542 33661-33674 NN denotes proliferation
T6543 33675-33678 MD denotes may
T6544 33679-33681 VB denotes be
T6545 33690-33692 IN denotes in
T6546 33693-33697 NN denotes skin
T6547 33698-33708 NN denotes epithelium
T6548 33708-33710 , denotes ,
T6549 33710-33723 JJ denotes proliferative
T6550 33724-33735 NNS denotes circuitries
T6551 33736-33745 VBG denotes involving
T6552 33746-33749 NNS denotes AJs
T6553 33750-33753 VBP denotes are
T6554 33760-33762 TO denotes to
T6555 33763-33765 VB denotes be
T6556 33766-33773 JJ denotes complex
T6557 33774-33777 CC denotes and
T6558 33778-33783 RB denotes often
T6559 33784-33794 VBN denotes interwoven
T6560 33794-33795 . denotes .
T6561 33795-33900 sentence denotes Future studies will be needed to systematically dissect these putative intricacies at a molecular level.
T6562 33796-33802 JJ denotes Future
T6563 33803-33810 NNS denotes studies
T6564 33819-33825 VBN denotes needed
T6565 33811-33815 MD denotes will
T6566 33816-33818 VB denotes be
T6567 33826-33828 TO denotes to
T6568 33844-33851 VB denotes dissect
T6569 33829-33843 RB denotes systematically
T6570 33852-33857 DT denotes these
T6571 33867-33878 NNS denotes intricacies
T6572 33858-33866 JJ denotes putative
T6573 33879-33881 IN denotes at
T6574 33882-33883 DT denotes a
T6575 33894-33899 NN denotes level
T6576 33884-33893 JJ denotes molecular
T6577 33899-33900 . denotes .
T7354 33902-33909 VBG denotes Probing
T7355 33950-33957 VBZ denotes Reveals
T7356 33910-33913 DT denotes the
T7357 33914-33924 NN denotes Regulation
T7358 33925-33927 IN denotes of
T7359 33928-33933 NN denotes Snail
T7360 33934-33938 NN denotes Gene
T7361 33939-33949 NN denotes Expression
T7362 33958-33960 DT denotes an
T7363 33971-33975 NN denotes Link
T7364 33961-33970 JJ denotes Essential
T7365 33976-33978 IN denotes to
T7366 33979-33982 NN denotes TGF
T7367 33983-33985 NN denotes β2
T7368 33982-33983 HYPH denotes -
T7369 33986-33995 NN denotes Signaling
T7370 33996-33998 IN denotes in
T7371 33999-34002 DT denotes the
T7372 34019-34022 NN denotes Bud
T7373 34003-34013 VBG denotes Developing
T7374 34014-34018 NN denotes Hair
T7375 34022-34155 sentence denotes The temporal spike of Snail mRNA expression in the hair bud prompted us to consider what factor(s) may be regulating the Snail gene.
T7376 34023-34026 DT denotes The
T7377 34036-34041 NN denotes spike
T7378 34027-34035 JJ denotes temporal
T7379 34083-34091 VBD denotes prompted
T7380 34042-34044 IN denotes of
T7381 34045-34050 NN denotes Snail
T7382 34056-34066 NN denotes expression
T7383 34051-34055 NN denotes mRNA
T7384 34067-34069 IN denotes in
T7385 34070-34073 DT denotes the
T7386 34079-34082 NN denotes bud
T7387 34074-34078 NN denotes hair
T7388 34092-34094 PRP denotes us
T7389 34095-34097 TO denotes to
T7390 34098-34106 VB denotes consider
T7391 34107-34111 WDT denotes what
T7392 34112-34118 NN denotes factor
T7393 34129-34139 VBG denotes regulating
T7394 34118-34119 -LRB- denotes (
T7395 34119-34120 AFX denotes s
T7396 34120-34121 -RRB- denotes )
T7397 34122-34125 MD denotes may
T7398 34126-34128 VB denotes be
T7399 34140-34143 DT denotes the
T7400 34150-34154 NN denotes gene
T7401 34144-34149 NN denotes Snail
T7402 34154-34155 . denotes .
T7403 34155-34379 sentence denotes A variety of extracellular signals have an impact on the cell type-specific expression of different Snail family members, and many of them, including Wnts, BMPs, FGFs, and TGF-βs, also affect hair bud development [2,16,28].
T7404 34156-34157 DT denotes A
T7405 34158-34165 NN denotes variety
T7406 34191-34195 VBP denotes have
T7407 34166-34168 IN denotes of
T7408 34169-34182 JJ denotes extracellular
T7409 34183-34190 NNS denotes signals
T7410 34196-34198 DT denotes an
T7411 34199-34205 NN denotes impact
T7412 34206-34208 IN denotes on
T7413 34209-34212 DT denotes the
T7414 34232-34242 NN denotes expression
T7415 34213-34217 NN denotes cell
T7416 34218-34222 NN denotes type
T7417 34223-34231 JJ denotes specific
T7418 34222-34223 HYPH denotes -
T7419 34243-34245 IN denotes of
T7420 34246-34255 JJ denotes different
T7421 34269-34276 NNS denotes members
T7422 34256-34261 NN denotes Snail
T7423 34262-34268 NN denotes family
T7424 34276-34278 , denotes ,
T7425 34278-34281 CC denotes and
T7426 34282-34286 JJ denotes many
T7427 34341-34347 VBP denotes affect
T7428 34287-34289 IN denotes of
T7429 34290-34294 PRP denotes them
T7430 34294-34296 , denotes ,
T7431 34296-34305 VBG denotes including
T7432 34306-34310 NNS denotes Wnts
T7433 34310-34312 , denotes ,
T7434 34312-34316 NNS denotes BMPs
T7435 34316-34318 , denotes ,
T7436 34318-34322 NNS denotes FGFs
T7437 34322-34324 , denotes ,
T7438 34324-34327 CC denotes and
T7439 34328-34331 NN denotes TGF
T7440 34332-34334 NNS denotes βs
T7441 34331-34332 HYPH denotes -
T7442 34334-34336 , denotes ,
T7443 34336-34340 RB denotes also
T7444 34348-34352 NN denotes hair
T7445 34353-34356 NN denotes bud
T7446 34357-34368 NN denotes development
T7447 34369-34370 -LRB- denotes [
T7448 34375-34377 CD denotes 28
T7449 34370-34371 CD denotes 2
T7450 34371-34372 , denotes ,
T7451 34372-34374 CD denotes 16
T7452 34374-34375 , denotes ,
T7453 34377-34378 -RRB- denotes ]
T7454 34378-34379 . denotes .
T7455 34379-34607 sentence denotes Since Snail is not expressed in cultured skin keratinocytes that secrete active BMPs and FGFs (see Figure 1B), we focused our attention on Wnt and TGF-β signaling as more likely candidates for Snail induction in this cell type.
T7456 34380-34385 IN denotes Since
T7457 34399-34408 VBN denotes expressed
T7458 34386-34391 NN denotes Snail
T7459 34392-34394 VBZ denotes is
T7460 34395-34398 RB denotes not
T7461 34494-34501 VBD denotes focused
T7462 34409-34411 IN denotes in
T7463 34412-34420 VBN denotes cultured
T7464 34426-34439 NNS denotes keratinocytes
T7465 34421-34425 NN denotes skin
T7466 34440-34444 WDT denotes that
T7467 34445-34452 VBP denotes secrete
T7468 34453-34459 JJ denotes active
T7469 34460-34464 NNS denotes BMPs
T7470 34465-34468 CC denotes and
T7471 34469-34473 NNS denotes FGFs
T7472 34474-34475 -LRB- denotes (
T7473 34475-34478 VB denotes see
T7474 34479-34485 NN denotes Figure
T7475 34486-34488 NN denotes 1B
T7476 34488-34489 -RRB- denotes )
T7477 34489-34491 , denotes ,
T7478 34491-34493 PRP denotes we
T7479 34502-34505 PRP$ denotes our
T7480 34506-34515 NN denotes attention
T7481 34516-34518 IN denotes on
T7482 34519-34522 NN denotes Wnt
T7483 34533-34542 NN denotes signaling
T7484 34523-34526 CC denotes and
T7485 34527-34530 NN denotes TGF
T7486 34531-34532 NN denotes β
T7487 34530-34531 HYPH denotes -
T7488 34543-34545 IN denotes as
T7489 34546-34550 RBR denotes more
T7490 34551-34557 JJ denotes likely
T7491 34558-34568 NNS denotes candidates
T7492 34569-34572 IN denotes for
T7493 34573-34578 NN denotes Snail
T7494 34579-34588 NN denotes induction
T7495 34589-34591 IN denotes in
T7496 34592-34596 DT denotes this
T7497 34602-34606 NN denotes type
T7498 34597-34601 NN denotes cell
T7499 34606-34607 . denotes .
T7500 34607-34806 sentence denotes Previously, we showed that effective transmission of a Wnt-3a signal in cultured keratinocytes can be achieved through their exposure to the BMP inhibitor noggin, which induces LEF-1 expression [4].
T7501 34608-34618 RB denotes Previously
T7502 34623-34629 VBD denotes showed
T7503 34618-34620 , denotes ,
T7504 34620-34622 PRP denotes we
T7505 34630-34634 IN denotes that
T7506 34710-34718 VBN denotes achieved
T7507 34635-34644 JJ denotes effective
T7508 34645-34657 NN denotes transmission
T7509 34658-34660 IN denotes of
T7510 34661-34662 DT denotes a
T7511 34670-34676 NN denotes signal
T7512 34663-34666 NN denotes Wnt
T7513 34666-34667 HYPH denotes -
T7514 34667-34669 CD denotes 3a
T7515 34677-34679 IN denotes in
T7516 34680-34688 VBN denotes cultured
T7517 34689-34702 NNS denotes keratinocytes
T7518 34703-34706 MD denotes can
T7519 34707-34709 VB denotes be
T7520 34719-34726 IN denotes through
T7521 34727-34732 PRP$ denotes their
T7522 34733-34741 NN denotes exposure
T7523 34742-34744 IN denotes to
T7524 34745-34748 DT denotes the
T7525 34753-34762 NN denotes inhibitor
T7526 34749-34752 NN denotes BMP
T7527 34763-34769 NN denotes noggin
T7528 34769-34771 , denotes ,
T7529 34771-34776 WDT denotes which
T7530 34777-34784 VBZ denotes induces
T7531 34785-34788 NN denotes LEF
T7532 34791-34801 NN denotes expression
T7533 34788-34789 HYPH denotes -
T7534 34789-34790 CD denotes 1
T7535 34802-34803 -LRB- denotes [
T7536 34803-34804 CD denotes 4
T7537 34804-34805 -RRB- denotes ]
T7538 34805-34806 . denotes .
T7539 34806-34941 sentence denotes In vitro, these conditions down-regulated the E-cadherin promoter and induced a LEF-1/β-catenin-sensitive reporter gene, TOPFLASH [4].
T7540 34807-34809 FW denotes In
T7541 34810-34815 FW denotes vitro
T7542 34839-34848 VBD denotes regulated
T7543 34815-34817 , denotes ,
T7544 34817-34822 DT denotes these
T7545 34823-34833 NNS denotes conditions
T7546 34834-34838 RB denotes down
T7547 34838-34839 HYPH denotes -
T7548 34849-34852 DT denotes the
T7549 34864-34872 NN denotes promoter
T7550 34853-34854 NN denotes E
T7551 34855-34863 NN denotes cadherin
T7552 34854-34855 HYPH denotes -
T7553 34873-34876 CC denotes and
T7554 34877-34884 VBD denotes induced
T7555 34885-34886 DT denotes a
T7556 34922-34926 NN denotes gene
T7557 34887-34890 NN denotes LEF
T7558 34903-34912 JJ denotes sensitive
T7559 34890-34891 HYPH denotes -
T7560 34891-34892 CD denotes 1
T7561 34892-34893 HYPH denotes /
T7562 34893-34894 NN denotes β
T7563 34895-34902 NN denotes catenin
T7564 34894-34895 HYPH denotes -
T7565 34902-34903 HYPH denotes -
T7566 34913-34921 NN denotes reporter
T7567 34926-34928 , denotes ,
T7568 34928-34936 NN denotes TOPFLASH
T7569 34937-34938 -LRB- denotes [
T7570 34938-34939 CD denotes 4
T7571 34939-34940 -RRB- denotes ]
T7572 34940-34941 . denotes .
T7573 34941-35020 sentence denotes In contrast, Snail expression was not induced by these conditions (Figure 5A).
T7574 34942-34944 IN denotes In
T7575 34980-34987 VBN denotes induced
T7576 34945-34953 NN denotes contrast
T7577 34953-34955 , denotes ,
T7578 34955-34960 NN denotes Snail
T7579 34961-34971 NN denotes expression
T7580 34972-34975 VBD denotes was
T7581 34976-34979 RB denotes not
T7582 34988-34990 IN denotes by
T7583 34991-34996 DT denotes these
T7584 34997-35007 NNS denotes conditions
T7585 35008-35009 -LRB- denotes (
T7586 35016-35018 NN denotes 5A
T7587 35009-35015 NN denotes Figure
T7588 35018-35019 -RRB- denotes )
T7589 35019-35020 . denotes .
T7590 35020-35224 sentence denotes Thus, despite essential roles for Wnts and noggin in hair follicle specification [4,29,30], our studies did not support an essential role for these signals in governing Snail expression in keratinocytes.
T7591 35021-35025 RB denotes Thus
T7592 35133-35140 VB denotes support
T7593 35025-35027 , denotes ,
T7594 35027-35034 IN denotes despite
T7595 35035-35044 JJ denotes essential
T7596 35045-35050 NNS denotes roles
T7597 35051-35054 IN denotes for
T7598 35055-35059 NNS denotes Wnts
T7599 35060-35063 CC denotes and
T7600 35064-35070 NN denotes noggin
T7601 35071-35073 IN denotes in
T7602 35074-35078 NN denotes hair
T7603 35079-35087 NN denotes follicle
T7604 35088-35101 NN denotes specification
T7605 35102-35103 -LRB- denotes [
T7606 35108-35110 CD denotes 30
T7607 35103-35104 CD denotes 4
T7608 35104-35105 , denotes ,
T7609 35105-35107 CD denotes 29
T7610 35107-35108 , denotes ,
T7611 35110-35111 -RRB- denotes ]
T7612 35111-35113 , denotes ,
T7613 35113-35116 PRP$ denotes our
T7614 35117-35124 NNS denotes studies
T7615 35125-35128 VBD denotes did
T7616 35129-35132 RB denotes not
T7617 35141-35143 DT denotes an
T7618 35154-35158 NN denotes role
T7619 35144-35153 JJ denotes essential
T7620 35159-35162 IN denotes for
T7621 35163-35168 DT denotes these
T7622 35169-35176 NNS denotes signals
T7623 35177-35179 IN denotes in
T7624 35180-35189 VBG denotes governing
T7625 35190-35195 NN denotes Snail
T7626 35196-35206 NN denotes expression
T7627 35207-35209 IN denotes in
T7628 35210-35223 NNS denotes keratinocytes
T7629 35223-35224 . denotes .
T7630 35224-37423 sentence denotes Figure 5 TGF-β2, but Not Wnt/noggin, Transiently Induces Snail Expression In Vitro (A) Failure of Wnt and noggin signaling to induce Snail in cultured keratinocytes. Primary mouse keratinocytes were treated with Wnt- and/or noggin-conditioned medium (+) or the corresponding control medium (–). These conditions are known to activate the LEF-1/β-catenin reporter TOPGal and down-regulate the E-cadherin promoter (see [4] for details). Using Western blot analyses, cellular proteins were then analyzed for Snail, LEF-1, β-catenin, and tubulin. Proteins from keratinocytes transfected with K14-Snail were used as a positive control for Snail expression. (B) TGF-β2 can induce Snail protein. Primary keratinocytes were treated for the indicated times with recombinant TGF-β2 (+) or heat inactivated TGF-β2 (–).Total cellular proteins were then isolated and analyzed by Western blot for Snail, pSMAD2 (reflective of activated TGF- signaling), and tubulin. Note the activation of Snail expression, peaking at 2 h post-TGF-β2 treatment and then disappearing thereafter. (C) Snail mRNA expression is transiently induced by TGF-β2. The experiment in (B) was repeated, and this time, total RNAs were isolated from keratinocytes treated with TGF-β2 for the indicated times. RT-PCR was then used with (+) or without (–) reverse transcriptase (RT) and with primer sets specific for Snail and GAPDH mRNAs. Note that Snail mRNA expression also peaked at 2 h, paralleling Snail protein. (D) TGF-β2 treatment results in enhanced activity of a Snail promoter-β-galactosidase reporter. Keratinocytes were transfected with a β-galactosidase reporter driven by a Snail promoter that is either WT (wt prom) or harbors a mutation in a putative pSMAD2/pSMAD4 binding site (mt prom). At 2 d posttransfection, cells were treated with either TGF-β or heat-inactivated TGF-β2 (inact) for the times indicated. β-galactosidase assays were then conducted, and results are reported as fold increase over a basal level of activity of 1. The experiment was repeated three times in triplicate, and error bars reflect variations in the results. TGF-β1 has been shown to induce Snail family members in hepatocytes and heart [15, 31].
T7631 37336-37339 NN denotes TGF
T7632 37340-37342 NN denotes β1
T7633 37339-37340 HYPH denotes -
T7634 37352-37357 VBN denotes shown
T7635 37343-37346 VBZ denotes has
T7636 37347-37351 VBN denotes been
T7637 37358-37360 TO denotes to
T7638 37361-37367 VB denotes induce
T7639 37368-37373 NN denotes Snail
T7640 37381-37388 NNS denotes members
T7641 37374-37380 NN denotes family
T7642 37389-37391 IN denotes in
T7643 37392-37403 NNS denotes hepatocytes
T7644 37404-37407 CC denotes and
T7645 37408-37413 NN denotes heart
T7646 37414-37415 -LRB- denotes [
T7647 37419-37421 CD denotes 31
T7648 37415-37417 CD denotes 15
T7649 37417-37419 , denotes ,
T7650 37421-37422 -RRB- denotes ]
T7651 37422-37423 . denotes .
T7652 37423-37582 sentence denotes In keratinocytes, however, TGF-β1 inhibits keratinocyte growth and seems to be involved in triggering the destructive phase of the cycling hair follicle [32].
T7653 37424-37426 IN denotes In
T7654 37458-37466 VBZ denotes inhibits
T7655 37427-37440 NNS denotes keratinocytes
T7656 37440-37442 , denotes ,
T7657 37442-37449 RB denotes however
T7658 37449-37451 , denotes ,
T7659 37451-37454 NN denotes TGF
T7660 37455-37457 NN denotes β1
T7661 37454-37455 HYPH denotes -
T7662 37467-37479 NN denotes keratinocyte
T7663 37480-37486 NN denotes growth
T7664 37487-37490 CC denotes and
T7665 37491-37496 VBZ denotes seems
T7666 37497-37499 TO denotes to
T7667 37503-37511 VBN denotes involved
T7668 37500-37502 VB denotes be
T7669 37512-37514 IN denotes in
T7670 37515-37525 VBG denotes triggering
T7671 37526-37529 DT denotes the
T7672 37542-37547 NN denotes phase
T7673 37530-37541 JJ denotes destructive
T7674 37548-37550 IN denotes of
T7675 37551-37554 DT denotes the
T7676 37568-37576 NN denotes follicle
T7677 37555-37562 VBG denotes cycling
T7678 37563-37567 NN denotes hair
T7679 37577-37578 -LRB- denotes [
T7680 37578-37580 CD denotes 32
T7681 37580-37581 -RRB- denotes ]
T7682 37581-37582 . denotes .
T7683 37582-37738 sentence denotes Of the loss-of-function mutations generated in each of the TGF-β genes, only the TGF-β2 null state blocked follicle development at the hair bud stage [32].
T7684 37583-37585 IN denotes Of
T7685 37682-37689 VBD denotes blocked
T7686 37586-37589 DT denotes the
T7687 37607-37616 NNS denotes mutations
T7688 37590-37594 NN denotes loss
T7689 37594-37595 HYPH denotes -
T7690 37595-37597 IN denotes of
T7691 37597-37598 HYPH denotes -
T7692 37598-37606 NN denotes function
T7693 37617-37626 VBN denotes generated
T7694 37627-37629 IN denotes in
T7695 37630-37634 DT denotes each
T7696 37635-37637 IN denotes of
T7697 37638-37641 DT denotes the
T7698 37648-37653 NNS denotes genes
T7699 37642-37645 NN denotes TGF
T7700 37646-37647 NN denotes β
T7701 37645-37646 HYPH denotes -
T7702 37653-37655 , denotes ,
T7703 37655-37659 RB denotes only
T7704 37676-37681 NN denotes state
T7705 37660-37663 DT denotes the
T7706 37664-37667 NN denotes TGF
T7707 37668-37670 NN denotes β2
T7708 37667-37668 HYPH denotes -
T7709 37671-37675 JJ denotes null
T7710 37690-37698 NN denotes follicle
T7711 37699-37710 NN denotes development
T7712 37711-37713 IN denotes at
T7713 37714-37717 DT denotes the
T7714 37727-37732 NN denotes stage
T7715 37718-37722 NN denotes hair
T7716 37723-37726 NN denotes bud
T7717 37733-37734 -LRB- denotes [
T7718 37734-37736 CD denotes 32
T7719 37736-37737 -RRB- denotes ]
T7720 37737-37738 . denotes .
T7721 37738-37902 sentence denotes Thus, we turned towards addressing whether TGF-β2 might be involved in regulating Snail expression in keratinocytes isolated from the basal layer of the epidermis.
T7722 37739-37743 RB denotes Thus
T7723 37748-37754 VBD denotes turned
T7724 37743-37745 , denotes ,
T7725 37745-37747 PRP denotes we
T7726 37755-37762 IN denotes towards
T7727 37763-37773 VBG denotes addressing
T7728 37774-37781 IN denotes whether
T7729 37798-37806 VBN denotes involved
T7730 37782-37785 NN denotes TGF
T7731 37786-37788 NN denotes β2
T7732 37785-37786 HYPH denotes -
T7733 37789-37794 MD denotes might
T7734 37795-37797 VB denotes be
T7735 37807-37809 IN denotes in
T7736 37810-37820 VBG denotes regulating
T7737 37821-37826 NN denotes Snail
T7738 37827-37837 NN denotes expression
T7739 37838-37840 IN denotes in
T7740 37841-37854 NNS denotes keratinocytes
T7741 37855-37863 VBN denotes isolated
T7742 37864-37868 IN denotes from
T7743 37869-37872 DT denotes the
T7744 37879-37884 NN denotes layer
T7745 37873-37878 JJ denotes basal
T7746 37885-37887 IN denotes of
T7747 37888-37891 DT denotes the
T7748 37892-37901 NN denotes epidermis
T7749 37901-37902 . denotes .
T7750 37902-38133 sentence denotes Though there is no cell culture system available to specifically study placodal cells, these keratinocytes are their progenitors and are the closest approximation available to study the behavior of epithelial cells of the placode.
T7751 37903-37909 IN denotes Though
T7752 37916-37918 VBZ denotes is
T7753 37910-37915 EX denotes there
T7754 38010-38013 VBP denotes are
T7755 37919-37921 DT denotes no
T7756 37935-37941 NN denotes system
T7757 37922-37926 NN denotes cell
T7758 37927-37934 NN denotes culture
T7759 37942-37951 JJ denotes available
T7760 37952-37954 TO denotes to
T7761 37968-37973 VB denotes study
T7762 37955-37967 RB denotes specifically
T7763 37974-37982 JJ denotes placodal
T7764 37983-37988 NNS denotes cells
T7765 37988-37990 , denotes ,
T7766 37990-37995 DT denotes these
T7767 37996-38009 NNS denotes keratinocytes
T7768 38014-38019 PRP$ denotes their
T7769 38020-38031 NNS denotes progenitors
T7770 38032-38035 CC denotes and
T7771 38036-38039 VBP denotes are
T7772 38040-38043 DT denotes the
T7773 38052-38065 NN denotes approximation
T7774 38044-38051 JJS denotes closest
T7775 38066-38075 JJ denotes available
T7776 38076-38078 TO denotes to
T7777 38079-38084 VB denotes study
T7778 38085-38088 DT denotes the
T7779 38089-38097 NN denotes behavior
T7780 38098-38100 IN denotes of
T7781 38101-38111 JJ denotes epithelial
T7782 38112-38117 NNS denotes cells
T7783 38118-38120 IN denotes of
T7784 38121-38124 DT denotes the
T7785 38125-38132 NN denotes placode
T7786 38132-38133 . denotes .
T7787 38133-38281 sentence denotes Interestingly, treatment of cultured keratinocytes with as little as 5 ng/ml of TGF-β2 caused a rapid and transient induction of Snail (Figure 5B).
T7788 38134-38147 RB denotes Interestingly
T7789 38221-38227 VBD denotes caused
T7790 38147-38149 , denotes ,
T7791 38149-38158 NN denotes treatment
T7792 38159-38161 IN denotes of
T7793 38162-38170 VBN denotes cultured
T7794 38171-38184 NNS denotes keratinocytes
T7795 38185-38189 IN denotes with
T7796 38190-38192 RB denotes as
T7797 38203-38204 CD denotes 5
T7798 38193-38199 JJ denotes little
T7799 38200-38202 IN denotes as
T7800 38205-38207 NN denotes ng
T7801 38207-38208 SYM denotes /
T7802 38208-38210 NN denotes ml
T7803 38211-38213 IN denotes of
T7804 38214-38217 NN denotes TGF
T7805 38218-38220 NN denotes β2
T7806 38217-38218 HYPH denotes -
T7807 38228-38229 DT denotes a
T7808 38250-38259 NN denotes induction
T7809 38230-38235 JJ denotes rapid
T7810 38236-38239 CC denotes and
T7811 38240-38249 JJ denotes transient
T7812 38260-38262 IN denotes of
T7813 38263-38268 NN denotes Snail
T7814 38269-38270 -LRB- denotes (
T7815 38277-38279 NN denotes 5B
T7816 38270-38276 NN denotes Figure
T7817 38279-38280 -RRB- denotes )
T7818 38280-38281 . denotes .
T7819 38281-38394 sentence denotes Following this treatment, Snail protein was detected within 30 min, peaked at 2 h, and then declined thereafter.
T7820 38282-38291 VBG denotes Following
T7821 38326-38334 VBN denotes detected
T7822 38292-38296 DT denotes this
T7823 38297-38306 NN denotes treatment
T7824 38306-38308 , denotes ,
T7825 38308-38313 NN denotes Snail
T7826 38314-38321 NN denotes protein
T7827 38322-38325 VBD denotes was
T7828 38335-38341 IN denotes within
T7829 38342-38344 CD denotes 30
T7830 38345-38348 NN denotes min
T7831 38348-38350 , denotes ,
T7832 38350-38356 VBD denotes peaked
T7833 38357-38359 IN denotes at
T7834 38360-38361 CD denotes 2
T7835 38362-38363 NN denotes h
T7836 38363-38365 , denotes ,
T7837 38365-38368 CC denotes and
T7838 38369-38373 RB denotes then
T7839 38374-38382 VBD denotes declined
T7840 38383-38393 RB denotes thereafter
T7841 38393-38394 . denotes .
T7842 38394-38586 sentence denotes The induction of Snail appeared to be specific for the active form of the growth factor, as pretreatment of TGF-β2 for 10 min at 100 °C obliterated the response [Figure 5B, lanes marked (–)].
T7843 38395-38398 DT denotes The
T7844 38399-38408 NN denotes induction
T7845 38418-38426 VBD denotes appeared
T7846 38409-38411 IN denotes of
T7847 38412-38417 NN denotes Snail
T7848 38427-38429 TO denotes to
T7849 38430-38432 VB denotes be
T7850 38433-38441 JJ denotes specific
T7851 38442-38445 IN denotes for
T7852 38446-38449 DT denotes the
T7853 38457-38461 NN denotes form
T7854 38450-38456 JJ denotes active
T7855 38462-38464 IN denotes of
T7856 38465-38468 DT denotes the
T7857 38476-38482 NN denotes factor
T7858 38469-38475 NN denotes growth
T7859 38482-38484 , denotes ,
T7860 38484-38486 IN denotes as
T7861 38531-38542 VBD denotes obliterated
T7862 38487-38499 NN denotes pretreatment
T7863 38500-38502 IN denotes of
T7864 38503-38506 NN denotes TGF
T7865 38507-38509 NN denotes β2
T7866 38506-38507 HYPH denotes -
T7867 38510-38513 IN denotes for
T7868 38514-38516 CD denotes 10
T7869 38517-38520 NN denotes min
T7870 38521-38523 IN denotes at
T7871 38524-38527 CD denotes 100
T7872 38528-38530 NN denotes °C
T7873 38543-38546 DT denotes the
T7874 38547-38555 NN denotes response
T7875 38556-38557 -LRB- denotes [
T7876 38568-38573 NNS denotes lanes
T7877 38557-38563 NN denotes Figure
T7878 38564-38566 NN denotes 5B
T7879 38566-38568 , denotes ,
T7880 38574-38580 VBN denotes marked
T7881 38581-38582 -LRB- denotes (
T7882 38582-38583 SYM denotes –
T7883 38583-38584 -RRB- denotes )
T7884 38584-38585 -RRB- denotes ]
T7885 38585-38586 . denotes .
T7886 38586-38824 sentence denotes By contrast, although TGF-β receptor activation remained elevated during the duration of the experiment (as measured by the sustained phosphorylation of the downstream effector SMAD2) Snail expression could not be maintained (Figure 5B).
T7887 38587-38589 IN denotes By
T7888 38801-38811 VBN denotes maintained
T7889 38590-38598 NN denotes contrast
T7890 38598-38600 , denotes ,
T7891 38600-38608 IN denotes although
T7892 38635-38643 VBD denotes remained
T7893 38609-38612 NN denotes TGF
T7894 38613-38614 NN denotes β
T7895 38612-38613 HYPH denotes -
T7896 38615-38623 NN denotes receptor
T7897 38624-38634 NN denotes activation
T7898 38644-38652 JJ denotes elevated
T7899 38653-38659 IN denotes during
T7900 38660-38663 DT denotes the
T7901 38664-38672 NN denotes duration
T7902 38673-38675 IN denotes of
T7903 38676-38679 DT denotes the
T7904 38680-38690 NN denotes experiment
T7905 38691-38692 -LRB- denotes (
T7906 38692-38694 IN denotes as
T7907 38695-38703 VBN denotes measured
T7908 38704-38706 IN denotes by
T7909 38707-38710 DT denotes the
T7910 38721-38736 NN denotes phosphorylation
T7911 38711-38720 JJ denotes sustained
T7912 38737-38739 IN denotes of
T7913 38740-38743 DT denotes the
T7914 38755-38763 NN denotes effector
T7915 38744-38754 JJ denotes downstream
T7916 38764-38769 NN denotes SMAD2
T7917 38769-38770 -RRB- denotes )
T7918 38771-38776 NN denotes Snail
T7919 38777-38787 NN denotes expression
T7920 38788-38793 MD denotes could
T7921 38794-38797 RB denotes not
T7922 38798-38800 VB denotes be
T7923 38812-38813 -LRB- denotes (
T7924 38820-38822 NN denotes 5B
T7925 38813-38819 NN denotes Figure
T7926 38822-38823 -RRB- denotes )
T7927 38823-38824 . denotes .
T7928 38824-38972 sentence denotes Thus, although Snail expression correlated with phosphorylated SMAD2 (pSMAD2) induction, its decline seemed to rely on secondary downstream events.
T7929 38825-38829 RB denotes Thus
T7930 38926-38932 VBD denotes seemed
T7931 38829-38831 , denotes ,
T7932 38831-38839 IN denotes although
T7933 38857-38867 VBD denotes correlated
T7934 38840-38845 NN denotes Snail
T7935 38846-38856 NN denotes expression
T7936 38868-38872 IN denotes with
T7937 38873-38887 VBN denotes phosphorylated
T7938 38888-38893 NN denotes SMAD2
T7939 38903-38912 NN denotes induction
T7940 38894-38895 -LRB- denotes (
T7941 38895-38901 NN denotes pSMAD2
T7942 38901-38902 -RRB- denotes )
T7943 38912-38914 , denotes ,
T7944 38914-38917 PRP$ denotes its
T7945 38918-38925 NN denotes decline
T7946 38933-38935 TO denotes to
T7947 38936-38940 VB denotes rely
T7948 38941-38943 IN denotes on
T7949 38944-38953 JJ denotes secondary
T7950 38965-38971 NNS denotes events
T7951 38954-38964 JJ denotes downstream
T7952 38971-38972 . denotes .
T7953 38972-39154 sentence denotes The rapid kinetics of Snail expression were reflected at the mRNA level, suggesting that Snail promoter activity in keratinocytes might be sensitive to TGF-β2 signaling (Figure 5C).
T7954 38973-38976 DT denotes The
T7955 38983-38991 NNS denotes kinetics
T7956 38977-38982 JJ denotes rapid
T7957 39017-39026 VBN denotes reflected
T7958 38992-38994 IN denotes of
T7959 38995-39000 NN denotes Snail
T7960 39001-39011 NN denotes expression
T7961 39012-39016 VBD denotes were
T7962 39027-39029 IN denotes at
T7963 39030-39033 DT denotes the
T7964 39039-39044 NN denotes level
T7965 39034-39038 NN denotes mRNA
T7966 39044-39046 , denotes ,
T7967 39046-39056 VBG denotes suggesting
T7968 39057-39061 IN denotes that
T7969 39109-39111 VB denotes be
T7970 39062-39067 NN denotes Snail
T7971 39068-39076 NN denotes promoter
T7972 39077-39085 NN denotes activity
T7973 39086-39088 IN denotes in
T7974 39089-39102 NNS denotes keratinocytes
T7975 39103-39108 MD denotes might
T7976 39112-39121 JJ denotes sensitive
T7977 39122-39124 IN denotes to
T7978 39125-39128 NN denotes TGF
T7979 39129-39131 NN denotes β2
T7980 39128-39129 HYPH denotes -
T7981 39132-39141 NN denotes signaling
T7982 39142-39143 -LRB- denotes (
T7983 39150-39152 NN denotes 5C
T7984 39143-39149 NN denotes Figure
T7985 39152-39153 -RRB- denotes )
T7986 39153-39154 . denotes .
T7987 39154-39381 sentence denotes To test this possibility, we engineered a transgene driving the β-galactosidase reporter under the control of approximately 2.2 kb of promoter sequence located 5′ from the transcription initiation site of the mouse Snail gene.
T7988 39155-39157 TO denotes To
T7989 39158-39162 VB denotes test
T7990 39184-39194 VBD denotes engineered
T7991 39163-39167 DT denotes this
T7992 39168-39179 NN denotes possibility
T7993 39179-39181 , denotes ,
T7994 39181-39183 PRP denotes we
T7995 39195-39196 DT denotes a
T7996 39197-39206 NN denotes transgene
T7997 39207-39214 VBG denotes driving
T7998 39215-39218 DT denotes the
T7999 39235-39243 NN denotes reporter
T8000 39219-39220 NN denotes β
T8001 39221-39234 NN denotes galactosidase
T8002 39220-39221 HYPH denotes -
T8003 39244-39249 IN denotes under
T8004 39250-39253 DT denotes the
T8005 39254-39261 NN denotes control
T8006 39262-39264 IN denotes of
T8007 39265-39278 RB denotes approximately
T8008 39279-39282 CD denotes 2.2
T8009 39283-39285 NN denotes kb
T8010 39286-39288 IN denotes of
T8011 39289-39297 NN denotes promoter
T8012 39298-39306 NN denotes sequence
T8013 39307-39314 VBN denotes located
T8014 39315-39316 CD denotes 5
T8015 39316-39317 SYM denotes ′
T8016 39318-39322 IN denotes from
T8017 39323-39326 DT denotes the
T8018 39352-39356 NN denotes site
T8019 39327-39340 NN denotes transcription
T8020 39341-39351 NN denotes initiation
T8021 39357-39359 IN denotes of
T8022 39360-39363 DT denotes the
T8023 39376-39380 NN denotes gene
T8024 39364-39369 NN denotes mouse
T8025 39370-39375 NN denotes Snail
T8026 39380-39381 . denotes .
T8027 39381-39581 sentence denotes At 2 d after transient transfection, keratinocytes were treated with TGF-β2 (t = 0) and then assayed for transgene activity over the same time course in which we had observed Snail protein induction.
T8028 39382-39384 IN denotes At
T8029 39438-39445 VBN denotes treated
T8030 39385-39386 CD denotes 2
T8031 39387-39388 NN denotes d
T8032 39389-39394 IN denotes after
T8033 39395-39404 JJ denotes transient
T8034 39405-39417 NN denotes transfection
T8035 39417-39419 , denotes ,
T8036 39419-39432 NNS denotes keratinocytes
T8037 39433-39437 VBD denotes were
T8038 39446-39450 IN denotes with
T8039 39451-39454 NN denotes TGF
T8040 39455-39457 NN denotes β2
T8041 39454-39455 HYPH denotes -
T8042 39458-39459 -LRB- denotes (
T8043 39463-39464 CD denotes 0
T8044 39459-39460 NN denotes t
T8045 39461-39462 SYM denotes =
T8046 39464-39465 -RRB- denotes )
T8047 39466-39469 CC denotes and
T8048 39470-39474 RB denotes then
T8049 39475-39482 VBN denotes assayed
T8050 39483-39486 IN denotes for
T8051 39487-39496 NN denotes transgene
T8052 39497-39505 NN denotes activity
T8053 39506-39510 IN denotes over
T8054 39511-39514 DT denotes the
T8055 39525-39531 NN denotes course
T8056 39515-39519 JJ denotes same
T8057 39520-39524 NN denotes time
T8058 39532-39534 IN denotes in
T8059 39548-39556 VBN denotes observed
T8060 39535-39540 WDT denotes which
T8061 39541-39543 PRP denotes we
T8062 39544-39547 VBD denotes had
T8063 39557-39562 NN denotes Snail
T8064 39563-39570 NN denotes protein
T8065 39571-39580 NN denotes induction
T8066 39580-39581 . denotes .
T8067 39581-39640 sentence denotes The results of this experiment are presented in Figure 5D.
T8068 39582-39585 DT denotes The
T8069 39586-39593 NNS denotes results
T8070 39617-39626 VBN denotes presented
T8071 39594-39596 IN denotes of
T8072 39597-39601 DT denotes this
T8073 39602-39612 NN denotes experiment
T8074 39613-39616 VBP denotes are
T8075 39627-39629 IN denotes in
T8076 39630-39636 NN denotes Figure
T8077 39637-39639 NN denotes 5D
T8078 39639-39640 . denotes .
T8079 39640-39800 sentence denotes Within 0.5 h of TGF-β2 treatment, Snail promoter activity had increased 3-fold, and by 2 h, it peaked to approximately 10-fold over control levels (Figure 5D).
T8080 39641-39647 IN denotes Within
T8081 39703-39712 VBN denotes increased
T8082 39648-39651 CD denotes 0.5
T8083 39652-39653 NN denotes h
T8084 39654-39656 IN denotes of
T8085 39657-39660 NN denotes TGF
T8086 39661-39663 NN denotes β2
T8087 39660-39661 HYPH denotes -
T8088 39664-39673 NN denotes treatment
T8089 39673-39675 , denotes ,
T8090 39675-39680 NN denotes Snail
T8091 39681-39689 NN denotes promoter
T8092 39690-39698 NN denotes activity
T8093 39699-39702 VBD denotes had
T8094 39713-39719 RB denotes 3-fold
T8095 39719-39721 , denotes ,
T8096 39721-39724 CC denotes and
T8097 39725-39727 IN denotes by
T8098 39736-39742 VBD denotes peaked
T8099 39728-39729 CD denotes 2
T8100 39730-39731 NN denotes h
T8101 39731-39733 , denotes ,
T8102 39733-39735 PRP denotes it
T8103 39743-39745 IN denotes to
T8104 39746-39759 RB denotes approximately
T8105 39760-39767 RB denotes 10-fold
T8106 39768-39772 IN denotes over
T8107 39773-39780 NN denotes control
T8108 39781-39787 NNS denotes levels
T8109 39788-39789 -LRB- denotes (
T8110 39796-39798 NN denotes 5D
T8111 39789-39795 NN denotes Figure
T8112 39798-39799 -RRB- denotes )
T8113 39799-39800 . denotes .
T8114 39800-39909 sentence denotes Thereafter, Snail promoter activity rapidly returned to the basal levels seen in unstimulated keratinocytes.
T8115 39801-39811 RB denotes Thereafter
T8116 39845-39853 VBD denotes returned
T8117 39811-39813 , denotes ,
T8118 39813-39818 NN denotes Snail
T8119 39819-39827 NN denotes promoter
T8120 39828-39836 NN denotes activity
T8121 39837-39844 RB denotes rapidly
T8122 39854-39856 IN denotes to
T8123 39857-39860 DT denotes the
T8124 39867-39873 NNS denotes levels
T8125 39861-39866 JJ denotes basal
T8126 39874-39878 VBN denotes seen
T8127 39879-39881 IN denotes in
T8128 39882-39894 JJ denotes unstimulated
T8129 39895-39908 NNS denotes keratinocytes
T8130 39908-39909 . denotes .
T8131 39909-40012 sentence denotes The kinetics of Snail promoter activity closely paralleled those observed for Snail protein induction.
T8132 39910-39913 DT denotes The
T8133 39914-39922 NNS denotes kinetics
T8134 39958-39968 VBD denotes paralleled
T8135 39923-39925 IN denotes of
T8136 39926-39931 NN denotes Snail
T8137 39932-39940 NN denotes promoter
T8138 39941-39949 NN denotes activity
T8139 39950-39957 RB denotes closely
T8140 39969-39974 DT denotes those
T8141 39975-39983 VBN denotes observed
T8142 39984-39987 IN denotes for
T8143 39988-39993 NN denotes Snail
T8144 39994-40001 NN denotes protein
T8145 40002-40011 NN denotes induction
T8146 40011-40012 . denotes .
T8147 40012-40284 sentence denotes Moreover, the stimulatory effects appeared to be specific to TGF-β2, since they were abrogated either by heat inactivation of the TGF-β2 protein or by mutation of a putative SMAD binding element located about 1.8 kb 5′ from the Snail transcription start site (Figure 5D).
T8148 40013-40021 RB denotes Moreover
T8149 40047-40055 VBD denotes appeared
T8150 40021-40023 , denotes ,
T8151 40023-40026 DT denotes the
T8152 40039-40046 NNS denotes effects
T8153 40027-40038 JJ denotes stimulatory
T8154 40056-40058 TO denotes to
T8155 40059-40061 VB denotes be
T8156 40062-40070 JJ denotes specific
T8157 40071-40073 IN denotes to
T8158 40074-40077 NN denotes TGF
T8159 40078-40080 NN denotes β2
T8160 40077-40078 HYPH denotes -
T8161 40080-40082 , denotes ,
T8162 40082-40087 IN denotes since
T8163 40098-40107 VBN denotes abrogated
T8164 40088-40092 PRP denotes they
T8165 40093-40097 VBD denotes were
T8166 40108-40114 CC denotes either
T8167 40115-40117 IN denotes by
T8168 40118-40122 NN denotes heat
T8169 40123-40135 NN denotes inactivation
T8170 40136-40138 IN denotes of
T8171 40139-40142 DT denotes the
T8172 40150-40157 NN denotes protein
T8173 40143-40146 NN denotes TGF
T8174 40147-40149 NN denotes β2
T8175 40146-40147 HYPH denotes -
T8176 40158-40160 CC denotes or
T8177 40161-40163 IN denotes by
T8178 40164-40172 NN denotes mutation
T8179 40173-40175 IN denotes of
T8180 40176-40177 DT denotes a
T8181 40200-40207 NN denotes element
T8182 40178-40186 JJ denotes putative
T8183 40187-40191 NN denotes SMAD
T8184 40192-40199 NN denotes binding
T8185 40208-40215 VBN denotes located
T8186 40216-40221 IN denotes about
T8187 40222-40225 CD denotes 1.8
T8188 40226-40228 NN denotes kb
T8189 40229-40230 CD denotes 5
T8190 40230-40231 SYM denotes ′
T8191 40232-40236 IN denotes from
T8192 40237-40240 DT denotes the
T8193 40267-40271 NN denotes site
T8194 40241-40246 NN denotes Snail
T8195 40247-40260 NN denotes transcription
T8196 40261-40266 NN denotes start
T8197 40272-40273 -LRB- denotes (
T8198 40280-40282 NN denotes 5D
T8199 40273-40279 NN denotes Figure
T8200 40282-40283 -RRB- denotes )
T8201 40283-40284 . denotes .
T8202 40284-40523 sentence denotes Taken together, these results suggested that in keratinocytes, TGF-β2 signaling results in a pSMAD2-dependent transient activation of the Snail gene, and that maintenance of Snail protein relies, in part, upon sustained promoter activity.
T8203 40285-40290 VBN denotes Taken
T8204 40315-40324 VBD denotes suggested
T8205 40291-40299 RB denotes together
T8206 40299-40301 , denotes ,
T8207 40301-40306 DT denotes these
T8208 40307-40314 NNS denotes results
T8209 40325-40329 IN denotes that
T8210 40365-40372 VBZ denotes results
T8211 40330-40332 IN denotes in
T8212 40333-40346 NNS denotes keratinocytes
T8213 40346-40348 , denotes ,
T8214 40348-40351 NN denotes TGF
T8215 40352-40354 NN denotes β2
T8216 40351-40352 HYPH denotes -
T8217 40355-40364 NN denotes signaling
T8218 40373-40375 IN denotes in
T8219 40376-40377 DT denotes a
T8220 40405-40415 NN denotes activation
T8221 40378-40384 NN denotes pSMAD2
T8222 40385-40394 JJ denotes dependent
T8223 40384-40385 HYPH denotes -
T8224 40395-40404 JJ denotes transient
T8225 40416-40418 IN denotes of
T8226 40419-40422 DT denotes the
T8227 40429-40433 NN denotes gene
T8228 40423-40428 NN denotes Snail
T8229 40433-40435 , denotes ,
T8230 40435-40438 CC denotes and
T8231 40439-40443 IN denotes that
T8232 40473-40479 VBZ denotes relies
T8233 40444-40455 NN denotes maintenance
T8234 40456-40458 IN denotes of
T8235 40459-40464 NN denotes Snail
T8236 40465-40472 NN denotes protein
T8237 40479-40481 , denotes ,
T8238 40481-40483 IN denotes in
T8239 40484-40488 NN denotes part
T8240 40488-40490 , denotes ,
T8241 40490-40494 IN denotes upon
T8242 40495-40504 JJ denotes sustained
T8243 40514-40522 NN denotes activity
T8244 40505-40513 NN denotes promoter
T8245 40522-40523 . denotes .
T8246 40523-40746 sentence denotes The brevity of Snail gene and protein induction in TGF-β2 treated cultured keratinocytes resembled the temporal appearance of Snail mRNA and protein at the initiation of hair follicle morphogenesis in embryonic mouse skin.
T8247 40524-40527 DT denotes The
T8248 40528-40535 NN denotes brevity
T8249 40613-40622 VBD denotes resembled
T8250 40536-40538 IN denotes of
T8251 40539-40544 NN denotes Snail
T8252 40545-40549 NN denotes gene
T8253 40562-40571 NN denotes induction
T8254 40550-40553 CC denotes and
T8255 40554-40561 NN denotes protein
T8256 40572-40574 IN denotes in
T8257 40575-40578 NN denotes TGF
T8258 40579-40581 NN denotes β2
T8259 40578-40579 HYPH denotes -
T8260 40582-40589 VBN denotes treated
T8261 40599-40612 NNS denotes keratinocytes
T8262 40590-40598 VBN denotes cultured
T8263 40623-40626 DT denotes the
T8264 40636-40646 NN denotes appearance
T8265 40627-40635 JJ denotes temporal
T8266 40647-40649 IN denotes of
T8267 40650-40655 NN denotes Snail
T8268 40656-40660 NN denotes mRNA
T8269 40661-40664 CC denotes and
T8270 40665-40672 NN denotes protein
T8271 40673-40675 IN denotes at
T8272 40676-40679 DT denotes the
T8273 40680-40690 NN denotes initiation
T8274 40691-40693 IN denotes of
T8275 40694-40698 NN denotes hair
T8276 40699-40707 NN denotes follicle
T8277 40708-40721 NN denotes morphogenesis
T8278 40722-40724 IN denotes in
T8279 40725-40734 JJ denotes embryonic
T8280 40741-40745 NN denotes skin
T8281 40735-40740 NN denotes mouse
T8282 40745-40746 . denotes .
T8283 40746-40912 sentence denotes To test whether TGF-β2 might be required for Snail induction in hair bud formation in vivo, we first analyzed whether TGF-β2 was expressed in or around the hair bud.
T8284 40747-40749 TO denotes To
T8285 40750-40754 VB denotes test
T8286 40848-40856 VBD denotes analyzed
T8287 40755-40762 IN denotes whether
T8288 40779-40787 VBN denotes required
T8289 40763-40766 NN denotes TGF
T8290 40767-40769 NN denotes β2
T8291 40766-40767 HYPH denotes -
T8292 40770-40775 MD denotes might
T8293 40776-40778 VB denotes be
T8294 40788-40791 IN denotes for
T8295 40792-40797 NN denotes Snail
T8296 40798-40807 NN denotes induction
T8297 40808-40810 IN denotes in
T8298 40811-40815 NN denotes hair
T8299 40816-40819 NN denotes bud
T8300 40820-40829 NN denotes formation
T8301 40830-40832 FW denotes in
T8302 40833-40837 FW denotes vivo
T8303 40837-40839 , denotes ,
T8304 40839-40841 PRP denotes we
T8305 40842-40847 RB denotes first
T8306 40857-40864 IN denotes whether
T8307 40876-40885 VBN denotes expressed
T8308 40865-40868 NN denotes TGF
T8309 40869-40871 NN denotes β2
T8310 40868-40869 HYPH denotes -
T8311 40872-40875 VBD denotes was
T8312 40886-40888 IN denotes in
T8313 40889-40891 CC denotes or
T8314 40892-40898 IN denotes around
T8315 40899-40902 DT denotes the
T8316 40908-40911 NN denotes bud
T8317 40903-40907 NN denotes hair
T8318 40911-40912 . denotes .
T8319 40912-41022 sentence denotes Consistent with previous observations [33], an anti-TGF-β2 antibody labeled developing hair buds (Figure 6A).
T8320 40913-40923 JJ denotes Consistent
T8321 40981-40988 VBD denotes labeled
T8322 40924-40928 IN denotes with
T8323 40929-40937 JJ denotes previous
T8324 40938-40950 NNS denotes observations
T8325 40951-40952 -LRB- denotes [
T8326 40952-40954 CD denotes 33
T8327 40954-40955 -RRB- denotes ]
T8328 40955-40957 , denotes ,
T8329 40957-40959 DT denotes an
T8330 40972-40980 NN denotes antibody
T8331 40960-40968 JJ denotes anti-TGF
T8332 40969-40971 NN denotes β2
T8333 40968-40969 HYPH denotes -
T8334 40989-40999 VBG denotes developing
T8335 41005-41009 NNS denotes buds
T8336 41000-41004 NN denotes hair
T8337 41010-41011 -LRB- denotes (
T8338 41018-41020 NN denotes 6A
T8339 41011-41017 NN denotes Figure
T8340 41020-41021 -RRB- denotes )
T8341 41021-41022 . denotes .
T8342 41022-41178 sentence denotes This labeling appeared to be specific as judged by the lack of staining in follicle buds from mice homozygous for a TGF-β2 null mutation (Figure 6A; [34]).
T8343 41023-41027 DT denotes This
T8344 41028-41036 NN denotes labeling
T8345 41037-41045 VBD denotes appeared
T8346 41046-41048 TO denotes to
T8347 41049-41051 VB denotes be
T8348 41052-41060 JJ denotes specific
T8349 41061-41063 IN denotes as
T8350 41064-41070 VBN denotes judged
T8351 41071-41073 IN denotes by
T8352 41074-41077 DT denotes the
T8353 41078-41082 NN denotes lack
T8354 41083-41085 IN denotes of
T8355 41086-41094 NN denotes staining
T8356 41095-41097 IN denotes in
T8357 41098-41106 NN denotes follicle
T8358 41107-41111 NNS denotes buds
T8359 41112-41116 IN denotes from
T8360 41117-41121 NNS denotes mice
T8361 41122-41132 JJ denotes homozygous
T8362 41133-41136 IN denotes for
T8363 41137-41138 DT denotes a
T8364 41151-41159 NN denotes mutation
T8365 41139-41142 NN denotes TGF
T8366 41143-41145 NN denotes β2
T8367 41142-41143 HYPH denotes -
T8368 41146-41150 JJ denotes null
T8369 41160-41161 -LRB- denotes (
T8370 41173-41175 CD denotes 34
T8371 41161-41167 NN denotes Figure
T8372 41168-41170 NN denotes 6A
T8373 41170-41171 : denotes ;
T8374 41172-41173 -LRB- denotes [
T8375 41175-41176 -RRB- denotes ]
T8376 41176-41177 -RRB- denotes )
T8377 41177-41178 . denotes .
T8378 41178-41311 sentence denotes Moreover, the downstream effector of TGF-β2 signaling, pSMAD2, was also expressed in WT, but not TGF-β2-null, hair buds (Figure 6B).
T8379 41179-41187 RB denotes Moreover
T8380 41251-41260 VBN denotes expressed
T8381 41187-41189 , denotes ,
T8382 41189-41192 DT denotes the
T8383 41204-41212 NN denotes effector
T8384 41193-41203 JJ denotes downstream
T8385 41213-41215 IN denotes of
T8386 41216-41219 NN denotes TGF
T8387 41220-41222 NN denotes β2
T8388 41219-41220 HYPH denotes -
T8389 41223-41232 NN denotes signaling
T8390 41232-41234 , denotes ,
T8391 41234-41240 NN denotes pSMAD2
T8392 41240-41242 , denotes ,
T8393 41242-41245 VBD denotes was
T8394 41246-41250 RB denotes also
T8395 41261-41263 IN denotes in
T8396 41264-41266 NN denotes WT
T8397 41294-41298 NNS denotes buds
T8398 41266-41268 , denotes ,
T8399 41268-41271 CC denotes but
T8400 41272-41275 RB denotes not
T8401 41276-41279 NN denotes TGF
T8402 41280-41282 NN denotes β2
T8403 41279-41280 HYPH denotes -
T8404 41283-41287 JJ denotes null
T8405 41282-41283 HYPH denotes -
T8406 41287-41289 , denotes ,
T8407 41289-41293 NN denotes hair
T8408 41299-41300 -LRB- denotes (
T8409 41307-41309 NN denotes 6B
T8410 41300-41306 NN denotes Figure
T8411 41309-41310 -RRB- denotes )
T8412 41310-41311 . denotes .
T8413 41311-41464 sentence denotes Together, these data underscore the importance of the TGF-β2 isoform despite expression of both TGF-β1 and TGF-β2 in developing hair buds at this stage.
T8414 41312-41320 RB denotes Together
T8415 41333-41343 VBP denotes underscore
T8416 41320-41322 , denotes ,
T8417 41322-41327 DT denotes these
T8418 41328-41332 NNS denotes data
T8419 41344-41347 DT denotes the
T8420 41348-41358 NN denotes importance
T8421 41359-41361 IN denotes of
T8422 41362-41365 DT denotes the
T8423 41373-41380 NN denotes isoform
T8424 41366-41369 NN denotes TGF
T8425 41370-41372 NN denotes β2
T8426 41369-41370 HYPH denotes -
T8427 41381-41388 IN denotes despite
T8428 41389-41399 NN denotes expression
T8429 41400-41402 IN denotes of
T8430 41403-41407 CC denotes both
T8431 41412-41414 NN denotes β1
T8432 41408-41411 NN denotes TGF
T8433 41411-41412 HYPH denotes -
T8434 41415-41418 CC denotes and
T8435 41419-41422 NN denotes TGF
T8436 41423-41425 NN denotes β2
T8437 41422-41423 HYPH denotes -
T8438 41426-41428 IN denotes in
T8439 41429-41439 VBG denotes developing
T8440 41445-41449 NNS denotes buds
T8441 41440-41444 NN denotes hair
T8442 41450-41452 IN denotes at
T8443 41453-41457 DT denotes this
T8444 41458-41463 NN denotes stage
T8445 41463-41464 . denotes .
T8446 41464-42595 sentence denotes Figure 6 TGF-β2 Is Necessary to Induce Snail Expression and Regulate Proliferation and E-Cadherin in the Hair Bud (A–D) Skins from TGF-β2 WT or KO E17.5 embryos were analyzed for expression of TGF-β2 protein (A), which is present in the epidermis and dermis as previously described [33] and in the hair bud, pSMAD2 (B), Snail (C), and Snail mRNA (D). Arrows point to the hair buds. (E) Western blot analyses of Snail expression in the skins of 2-wk-old K14-Smad2 transgenic (SMAD2 TG) and WT littermate (WT) mice. Antibody to tubulin was used as a control for equal protein loadings. The K14-Smad2 Tg mouse was previously shown to possess activated TGF-β signaling [35]. (F–G) Proliferation markers Ki67 (F) and pMAPK (G) are diminished in TGF-β2-null hair relative to its WT counterpart. (H–J) TGF-β2-null hair fails to down-regulate E-cadherin (H). Wnt and noggin signaling pathways are still intact in the TGF-β2 null hair as nuclear LEF-1 (I) and nuclear β-catenin (J) are still expressed. To further explore the possible relation between Snail and TGF-β2, we examined the status of Snail expression in TGF-β2-null hair buds.
T8447 42460-42462 TO denotes To
T8448 42471-42478 VB denotes explore
T8449 42463-42470 RB denotes further
T8450 42530-42538 VBD denotes examined
T8451 42479-42482 DT denotes the
T8452 42492-42500 NN denotes relation
T8453 42483-42491 JJ denotes possible
T8454 42501-42508 IN denotes between
T8455 42509-42514 NN denotes Snail
T8456 42515-42518 CC denotes and
T8457 42519-42522 NN denotes TGF
T8458 42523-42525 NN denotes β2
T8459 42522-42523 HYPH denotes -
T8460 42525-42527 , denotes ,
T8461 42527-42529 PRP denotes we
T8462 42539-42542 DT denotes the
T8463 42543-42549 NN denotes status
T8464 42550-42552 IN denotes of
T8465 42553-42558 NN denotes Snail
T8466 42559-42569 NN denotes expression
T8467 42570-42572 IN denotes in
T8468 42573-42576 NN denotes TGF
T8469 42577-42579 NN denotes β2
T8470 42576-42577 HYPH denotes -
T8471 42580-42584 JJ denotes null
T8472 42579-42580 HYPH denotes -
T8473 42590-42594 NNS denotes buds
T8474 42585-42589 NN denotes hair
T8475 42594-42595 . denotes .
T8476 42595-42748 sentence denotes As judged by immunohistochemistry, Snail protein was absent from E17.5 skin of TGF-β2-null embryos but not from that of control littermates (Figure 6C).
T8477 42596-42598 IN denotes As
T8478 42599-42605 VBN denotes judged
T8479 42645-42648 VBD denotes was
T8480 42606-42608 IN denotes by
T8481 42609-42629 NN denotes immunohistochemistry
T8482 42629-42631 , denotes ,
T8483 42631-42636 NN denotes Snail
T8484 42637-42644 NN denotes protein
T8485 42649-42655 JJ denotes absent
T8486 42656-42660 IN denotes from
T8487 42661-42666 NN denotes E17.5
T8488 42667-42671 NN denotes skin
T8489 42672-42674 IN denotes of
T8490 42675-42678 NN denotes TGF
T8491 42679-42681 NN denotes β2
T8492 42678-42679 HYPH denotes -
T8493 42682-42686 JJ denotes null
T8494 42681-42682 HYPH denotes -
T8495 42687-42694 NNS denotes embryos
T8496 42695-42698 CC denotes but
T8497 42699-42702 RB denotes not
T8498 42703-42707 IN denotes from
T8499 42708-42712 DT denotes that
T8500 42713-42715 IN denotes of
T8501 42716-42723 NN denotes control
T8502 42724-42735 NNS denotes littermates
T8503 42736-42737 -LRB- denotes (
T8504 42744-42746 NN denotes 6C
T8505 42737-42743 NN denotes Figure
T8506 42746-42747 -RRB- denotes )
T8507 42747-42748 . denotes .
T8508 42748-42983 sentence denotes This effect appeared to be exerted at the transcriptional level, since Snail mRNAs were also not found in TGF-β2 null hair buds under conditions in which the signal was readily detected in the hair buds of littermate skin (Figure 6D).
T8509 42749-42753 DT denotes This
T8510 42754-42760 NN denotes effect
T8511 42761-42769 VBD denotes appeared
T8512 42770-42772 TO denotes to
T8513 42776-42783 VBN denotes exerted
T8514 42773-42775 VB denotes be
T8515 42784-42786 IN denotes at
T8516 42787-42790 DT denotes the
T8517 42807-42812 NN denotes level
T8518 42791-42806 JJ denotes transcriptional
T8519 42812-42814 , denotes ,
T8520 42814-42819 IN denotes since
T8521 42846-42851 VBN denotes found
T8522 42820-42825 NN denotes Snail
T8523 42826-42831 NNS denotes mRNAs
T8524 42832-42836 VBD denotes were
T8525 42837-42841 RB denotes also
T8526 42842-42845 RB denotes not
T8527 42852-42854 IN denotes in
T8528 42855-42858 NN denotes TGF
T8529 42859-42861 NN denotes β2
T8530 42858-42859 HYPH denotes -
T8531 42862-42866 JJ denotes null
T8532 42872-42876 NNS denotes buds
T8533 42867-42871 NN denotes hair
T8534 42877-42882 IN denotes under
T8535 42883-42893 NNS denotes conditions
T8536 42894-42896 IN denotes in
T8537 42926-42934 VBN denotes detected
T8538 42897-42902 WDT denotes which
T8539 42903-42906 DT denotes the
T8540 42907-42913 NN denotes signal
T8541 42914-42917 VBD denotes was
T8542 42918-42925 RB denotes readily
T8543 42935-42937 IN denotes in
T8544 42938-42941 DT denotes the
T8545 42947-42951 NNS denotes buds
T8546 42942-42946 NN denotes hair
T8547 42952-42954 IN denotes of
T8548 42955-42965 NN denotes littermate
T8549 42966-42970 NN denotes skin
T8550 42971-42972 -LRB- denotes (
T8551 42979-42981 NN denotes 6D
T8552 42972-42978 NN denotes Figure
T8553 42981-42982 -RRB- denotes )
T8554 42982-42983 . denotes .
T8555 42983-43194 sentence denotes Conversely, in 2-wk-old K14-Smad2 Tg mice, which display elevated TGF-β signaling in skin [35], Snail protein was readily detected by Western blot analyses, where it was not found in postnatal skin (Figure 6E).
T8556 42984-42994 RB denotes Conversely
T8557 43106-43114 VBN denotes detected
T8558 42994-42996 , denotes ,
T8559 42996-42998 IN denotes in
T8560 42999-43000 CD denotes 2
T8561 43001-43003 NN denotes wk
T8562 43000-43001 HYPH denotes -
T8563 43004-43007 JJ denotes old
T8564 43003-43004 HYPH denotes -
T8565 43021-43025 NNS denotes mice
T8566 43008-43011 NN denotes K14
T8567 43012-43017 NN denotes Smad2
T8568 43011-43012 HYPH denotes -
T8569 43018-43020 NN denotes Tg
T8570 43025-43027 , denotes ,
T8571 43027-43032 WDT denotes which
T8572 43033-43040 VBP denotes display
T8573 43041-43049 VBN denotes elevated
T8574 43056-43065 NN denotes signaling
T8575 43050-43053 NN denotes TGF
T8576 43054-43055 NN denotes β
T8577 43053-43054 HYPH denotes -
T8578 43066-43068 IN denotes in
T8579 43069-43073 NN denotes skin
T8580 43074-43075 -LRB- denotes [
T8581 43075-43077 CD denotes 35
T8582 43077-43078 -RRB- denotes ]
T8583 43078-43080 , denotes ,
T8584 43080-43085 NN denotes Snail
T8585 43086-43093 NN denotes protein
T8586 43094-43097 VBD denotes was
T8587 43098-43105 RB denotes readily
T8588 43115-43117 IN denotes by
T8589 43118-43125 NNP denotes Western
T8590 43126-43130 NN denotes blot
T8591 43131-43139 NNS denotes analyses
T8592 43139-43141 , denotes ,
T8593 43141-43146 WRB denotes where
T8594 43158-43163 VBN denotes found
T8595 43147-43149 PRP denotes it
T8596 43150-43153 VBD denotes was
T8597 43154-43157 RB denotes not
T8598 43164-43166 IN denotes in
T8599 43167-43176 JJ denotes postnatal
T8600 43177-43181 NN denotes skin
T8601 43182-43183 -LRB- denotes (
T8602 43190-43192 NN denotes 6E
T8603 43183-43189 NN denotes Figure
T8604 43192-43193 -RRB- denotes )
T8605 43193-43194 . denotes .
T8606 43194-43378 sentence denotes Taken together, these results provide compelling evidence that TGF-β2 is functionally important for inducing Snail gene expression in a pSMAD-dependent manner in developing hair buds.
T8607 43195-43200 VBN denotes Taken
T8608 43225-43232 VBP denotes provide
T8609 43201-43209 RB denotes together
T8610 43209-43211 , denotes ,
T8611 43211-43216 DT denotes these
T8612 43217-43224 NNS denotes results
T8613 43233-43243 JJ denotes compelling
T8614 43244-43252 NN denotes evidence
T8615 43253-43257 IN denotes that
T8616 43265-43267 VBZ denotes is
T8617 43258-43261 NN denotes TGF
T8618 43262-43264 NN denotes β2
T8619 43261-43262 HYPH denotes -
T8620 43268-43280 RB denotes functionally
T8621 43281-43290 JJ denotes important
T8622 43291-43294 IN denotes for
T8623 43295-43303 VBG denotes inducing
T8624 43304-43309 NN denotes Snail
T8625 43315-43325 NN denotes expression
T8626 43310-43314 NN denotes gene
T8627 43326-43328 IN denotes in
T8628 43329-43330 DT denotes a
T8629 43347-43353 NN denotes manner
T8630 43331-43336 NN denotes pSMAD
T8631 43337-43346 JJ denotes dependent
T8632 43336-43337 HYPH denotes -
T8633 43354-43356 IN denotes in
T8634 43357-43367 VBG denotes developing
T8635 43373-43377 NNS denotes buds
T8636 43368-43372 NN denotes hair
T8637 43377-43378 . denotes .
T8638 43378-43482 sentence denotes Whether pMARK activity also contributes to Snail induction was not addressed in the present study [15].
T8639 43379-43386 IN denotes Whether
T8640 43407-43418 VBZ denotes contributes
T8641 43387-43392 NN denotes pMARK
T8642 43393-43401 NN denotes activity
T8643 43402-43406 RB denotes also
T8644 43446-43455 VBN denotes addressed
T8645 43419-43421 IN denotes to
T8646 43422-43427 NN denotes Snail
T8647 43428-43437 NN denotes induction
T8648 43438-43441 VBD denotes was
T8649 43442-43445 RB denotes not
T8650 43456-43458 IN denotes in
T8651 43459-43462 DT denotes the
T8652 43471-43476 NN denotes study
T8653 43463-43470 JJ denotes present
T8654 43477-43478 -LRB- denotes [
T8655 43478-43480 CD denotes 15
T8656 43480-43481 -RRB- denotes ]
T8657 43481-43482 . denotes .
T8658 43482-43592 sentence denotes Although some hair buds still formed in TGF-β2 null skin, their number was reduced by approximately 50% [32].
T8659 43483-43491 IN denotes Although
T8660 43513-43519 VBD denotes formed
T8661 43492-43496 DT denotes some
T8662 43502-43506 NNS denotes buds
T8663 43497-43501 NN denotes hair
T8664 43507-43512 RB denotes still
T8665 43558-43565 VBN denotes reduced
T8666 43520-43522 IN denotes in
T8667 43523-43526 NN denotes TGF
T8668 43527-43529 NN denotes β2
T8669 43526-43527 HYPH denotes -
T8670 43530-43534 JJ denotes null
T8671 43535-43539 NN denotes skin
T8672 43539-43541 , denotes ,
T8673 43541-43546 PRP$ denotes their
T8674 43547-43553 NN denotes number
T8675 43554-43557 VBD denotes was
T8676 43566-43568 IN denotes by
T8677 43569-43582 RB denotes approximately
T8678 43583-43585 CD denotes 50
T8679 43585-43586 NN denotes %
T8680 43587-43588 -LRB- denotes [
T8681 43588-43590 CD denotes 32
T8682 43590-43591 -RRB- denotes ]
T8683 43591-43592 . denotes .
T8684 43592-43760 sentence denotes Thus, although the pathway mediated by TGF-β2 signaling impacts the earliest step of epithelial invagination, it does not appear to be essential for bud morphogenesis.
T8685 43593-43597 RB denotes Thus
T8686 43715-43721 VB denotes appear
T8687 43597-43599 , denotes ,
T8688 43599-43607 IN denotes although
T8689 43649-43656 VBZ denotes impacts
T8690 43608-43611 DT denotes the
T8691 43612-43619 NN denotes pathway
T8692 43620-43628 VBN denotes mediated
T8693 43629-43631 IN denotes by
T8694 43632-43635 NN denotes TGF
T8695 43636-43638 NN denotes β2
T8696 43635-43636 HYPH denotes -
T8697 43639-43648 NN denotes signaling
T8698 43657-43660 DT denotes the
T8699 43670-43674 NN denotes step
T8700 43661-43669 JJS denotes earliest
T8701 43675-43677 IN denotes of
T8702 43678-43688 JJ denotes epithelial
T8703 43689-43701 NN denotes invagination
T8704 43701-43703 , denotes ,
T8705 43703-43705 PRP denotes it
T8706 43706-43710 VBZ denotes does
T8707 43711-43714 RB denotes not
T8708 43722-43724 TO denotes to
T8709 43725-43727 VB denotes be
T8710 43728-43737 JJ denotes essential
T8711 43738-43741 IN denotes for
T8712 43742-43745 NN denotes bud
T8713 43746-43759 NN denotes morphogenesis
T8714 43759-43760 . denotes .
T8715 43760-44034 sentence denotes Consistent with this notion, basement membrane remodeling still took place in the TGF-β2-null buds, as judged by immunofluorescence with antibodies against β4 integrin, an integral component of keratinocyte-mediated adhesion to its underlying basement membrane (Figure 6F).
T8716 43761-43771 JJ denotes Consistent
T8717 43825-43829 VBD denotes took
T8718 43772-43776 IN denotes with
T8719 43777-43781 DT denotes this
T8720 43782-43788 NN denotes notion
T8721 43788-43790 , denotes ,
T8722 43790-43798 NN denotes basement
T8723 43799-43807 NN denotes membrane
T8724 43808-43818 NN denotes remodeling
T8725 43819-43824 RB denotes still
T8726 43830-43835 RP denotes place
T8727 43836-43838 IN denotes in
T8728 43839-43842 DT denotes the
T8729 43855-43859 NNS denotes buds
T8730 43843-43846 NN denotes TGF
T8731 43847-43849 NN denotes β2
T8732 43846-43847 HYPH denotes -
T8733 43850-43854 JJ denotes null
T8734 43849-43850 HYPH denotes -
T8735 43859-43861 , denotes ,
T8736 43861-43863 IN denotes as
T8737 43864-43870 VBN denotes judged
T8738 43871-43873 IN denotes by
T8739 43874-43892 NN denotes immunofluorescence
T8740 43893-43897 IN denotes with
T8741 43898-43908 NNS denotes antibodies
T8742 43909-43916 IN denotes against
T8743 43917-43919 NN denotes β4
T8744 43920-43928 NN denotes integrin
T8745 43928-43930 , denotes ,
T8746 43930-43932 DT denotes an
T8747 43942-43951 NN denotes component
T8748 43933-43941 JJ denotes integral
T8749 43952-43954 IN denotes of
T8750 43955-43967 NN denotes keratinocyte
T8751 43968-43976 VBN denotes mediated
T8752 43967-43968 HYPH denotes -
T8753 43977-43985 NN denotes adhesion
T8754 43986-43988 IN denotes to
T8755 43989-43992 PRP$ denotes its
T8756 44013-44021 NN denotes membrane
T8757 43993-44003 JJ denotes underlying
T8758 44004-44012 NN denotes basement
T8759 44022-44023 -LRB- denotes (
T8760 44030-44032 NN denotes 6F
T8761 44023-44029 NN denotes Figure
T8762 44032-44033 -RRB- denotes )
T8763 44033-44034 . denotes .
T8764 44034-44245 sentence denotes In contrast, TGF-β2 signaling appeared to be an important factor for the early proliferation that occurs in the developing hair buds, as judged by anti-Ki67 and anti-pMAPK immunofluorescence (Figure 6F and 6G).
T8765 44035-44037 IN denotes In
T8766 44065-44073 VBD denotes appeared
T8767 44038-44046 NN denotes contrast
T8768 44046-44048 , denotes ,
T8769 44048-44051 NN denotes TGF
T8770 44052-44054 NN denotes β2
T8771 44051-44052 HYPH denotes -
T8772 44055-44064 NN denotes signaling
T8773 44074-44076 TO denotes to
T8774 44077-44079 VB denotes be
T8775 44080-44082 DT denotes an
T8776 44093-44099 NN denotes factor
T8777 44083-44092 JJ denotes important
T8778 44100-44103 IN denotes for
T8779 44104-44107 DT denotes the
T8780 44114-44127 NN denotes proliferation
T8781 44108-44113 JJ denotes early
T8782 44128-44132 WDT denotes that
T8783 44133-44139 VBZ denotes occurs
T8784 44140-44142 IN denotes in
T8785 44143-44146 DT denotes the
T8786 44163-44167 NNS denotes buds
T8787 44147-44157 VBG denotes developing
T8788 44158-44162 NN denotes hair
T8789 44167-44169 , denotes ,
T8790 44169-44171 IN denotes as
T8791 44172-44178 VBN denotes judged
T8792 44179-44181 IN denotes by
T8793 44182-44191 JJ denotes anti-Ki67
T8794 44207-44225 NN denotes immunofluorescence
T8795 44192-44195 CC denotes and
T8796 44196-44206 JJ denotes anti-pMAPK
T8797 44226-44227 -LRB- denotes (
T8798 44234-44236 NN denotes 6F
T8799 44227-44233 NN denotes Figure
T8800 44237-44240 CC denotes and
T8801 44241-44243 NN denotes 6G
T8802 44243-44244 -RRB- denotes )
T8803 44244-44245 . denotes .
T8804 44245-44417 sentence denotes If TGF-β2 stimulates Snail expression in developing buds, loss of this morphogen would be expected to affect the expression of genes that are typically repressed by Snail.
T8805 44246-44248 IN denotes If
T8806 44256-44266 VBZ denotes stimulates
T8807 44249-44252 NN denotes TGF
T8808 44253-44255 NN denotes β2
T8809 44252-44253 HYPH denotes -
T8810 44336-44344 VBN denotes expected
T8811 44267-44272 NN denotes Snail
T8812 44273-44283 NN denotes expression
T8813 44284-44286 IN denotes in
T8814 44287-44297 VBG denotes developing
T8815 44298-44302 NNS denotes buds
T8816 44302-44304 , denotes ,
T8817 44304-44308 NN denotes loss
T8818 44309-44311 IN denotes of
T8819 44312-44316 DT denotes this
T8820 44317-44326 NN denotes morphogen
T8821 44327-44332 MD denotes would
T8822 44333-44335 VB denotes be
T8823 44345-44347 TO denotes to
T8824 44348-44354 VB denotes affect
T8825 44355-44358 DT denotes the
T8826 44359-44369 NN denotes expression
T8827 44370-44372 IN denotes of
T8828 44373-44378 NNS denotes genes
T8829 44379-44383 WDT denotes that
T8830 44398-44407 VBN denotes repressed
T8831 44384-44387 VBP denotes are
T8832 44388-44397 RB denotes typically
T8833 44408-44410 IN denotes by
T8834 44411-44416 NN denotes Snail
T8835 44416-44417 . denotes .
T8836 44417-44562 sentence denotes Since a major target for Snail-mediated repression is the E-cadherin gene [12,13], we investigated the status of E-cadherin in TGF-β2-null buds.
T8837 44418-44423 IN denotes Since
T8838 44469-44471 VBZ denotes is
T8839 44424-44425 DT denotes a
T8840 44432-44438 NN denotes target
T8841 44426-44431 JJ denotes major
T8842 44439-44442 IN denotes for
T8843 44443-44448 NN denotes Snail
T8844 44449-44457 VBN denotes mediated
T8845 44448-44449 HYPH denotes -
T8846 44458-44468 NN denotes repression
T8847 44504-44516 VBD denotes investigated
T8848 44472-44475 DT denotes the
T8849 44487-44491 NN denotes gene
T8850 44476-44477 NN denotes E
T8851 44478-44486 NN denotes cadherin
T8852 44477-44478 HYPH denotes -
T8853 44492-44493 -LRB- denotes [
T8854 44496-44498 CD denotes 13
T8855 44493-44495 CD denotes 12
T8856 44495-44496 , denotes ,
T8857 44498-44499 -RRB- denotes ]
T8858 44499-44501 , denotes ,
T8859 44501-44503 PRP denotes we
T8860 44517-44520 DT denotes the
T8861 44521-44527 NN denotes status
T8862 44528-44530 IN denotes of
T8863 44531-44532 NN denotes E
T8864 44533-44541 NN denotes cadherin
T8865 44532-44533 HYPH denotes -
T8866 44542-44544 IN denotes in
T8867 44545-44548 NN denotes TGF
T8868 44549-44551 NN denotes β2
T8869 44548-44549 HYPH denotes -
T8870 44552-44556 JJ denotes null
T8871 44551-44552 HYPH denotes -
T8872 44557-44561 NNS denotes buds
T8873 44561-44562 . denotes .
T8874 44562-44697 sentence denotes As shown in Figure 6H, hair buds in TGF-β2 null skin displayed elevated immunofluorescence staining relative to their WT counterparts.
T8875 44563-44565 IN denotes As
T8876 44566-44571 VBN denotes shown
T8877 44616-44625 VBD denotes displayed
T8878 44572-44574 IN denotes in
T8879 44575-44581 NN denotes Figure
T8880 44582-44584 NN denotes 6H
T8881 44584-44586 , denotes ,
T8882 44586-44590 NN denotes hair
T8883 44591-44595 NNS denotes buds
T8884 44596-44598 IN denotes in
T8885 44599-44602 NN denotes TGF
T8886 44603-44605 NN denotes β2
T8887 44602-44603 HYPH denotes -
T8888 44606-44610 JJ denotes null
T8889 44611-44615 NN denotes skin
T8890 44626-44634 JJ denotes elevated
T8891 44654-44662 NN denotes staining
T8892 44635-44653 NN denotes immunofluorescence
T8893 44663-44671 JJ denotes relative
T8894 44672-44674 IN denotes to
T8895 44675-44680 PRP$ denotes their
T8896 44684-44696 NNS denotes counterparts
T8897 44681-44683 NN denotes WT
T8898 44696-44697 . denotes .
T8899 44697-44946 sentence denotes Previously we demonstrated that the concerted action of the extracellular signals Wnt and noggin are required for the generation of a LEF-1/β-catenin transcription complex to repress E-cadherin transcription at the onset of hair fate specification.
T8900 44698-44708 RB denotes Previously
T8901 44712-44724 VBD denotes demonstrated
T8902 44709-44711 PRP denotes we
T8903 44725-44729 IN denotes that
T8904 44799-44807 VBN denotes required
T8905 44730-44733 DT denotes the
T8906 44744-44750 NN denotes action
T8907 44734-44743 JJ denotes concerted
T8908 44751-44753 IN denotes of
T8909 44754-44757 DT denotes the
T8910 44772-44779 NNS denotes signals
T8911 44758-44771 JJ denotes extracellular
T8912 44780-44783 NN denotes Wnt
T8913 44784-44787 CC denotes and
T8914 44788-44794 NN denotes noggin
T8915 44795-44798 VBP denotes are
T8916 44808-44811 IN denotes for
T8917 44873-44880 VB denotes repress
T8918 44812-44815 DT denotes the
T8919 44816-44826 NN denotes generation
T8920 44827-44829 IN denotes of
T8921 44830-44831 DT denotes a
T8922 44862-44869 NN denotes complex
T8923 44832-44835 NN denotes LEF
T8924 44835-44836 HYPH denotes -
T8925 44836-44837 CD denotes 1
T8926 44837-44838 HYPH denotes /
T8927 44838-44839 NN denotes β
T8928 44840-44847 NN denotes catenin
T8929 44839-44840 HYPH denotes -
T8930 44848-44861 NN denotes transcription
T8931 44870-44872 TO denotes to
T8932 44881-44882 NN denotes E
T8933 44883-44891 NN denotes cadherin
T8934 44882-44883 HYPH denotes -
T8935 44892-44905 NN denotes transcription
T8936 44906-44908 IN denotes at
T8937 44909-44912 DT denotes the
T8938 44913-44918 NN denotes onset
T8939 44919-44921 IN denotes of
T8940 44922-44926 NN denotes hair
T8941 44927-44931 NN denotes fate
T8942 44932-44945 NN denotes specification
T8943 44945-44946 . denotes .
T8944 44946-45113 sentence denotes As shown in Figure 6I and 6J, both WT and TGF-β2 null buds exhibited nuclear LEF-1 and β-catenin localization, signs that the Wnt-noggin signaling pathway was intact.
T8945 44947-44949 IN denotes As
T8946 44950-44955 VBN denotes shown
T8947 45006-45015 VBD denotes exhibited
T8948 44956-44958 IN denotes in
T8949 44959-44965 NN denotes Figure
T8950 44966-44968 NN denotes 6I
T8951 44969-44972 CC denotes and
T8952 44973-44975 NN denotes 6J
T8953 44975-44977 , denotes ,
T8954 44977-44981 CC denotes both
T8955 44982-44984 NN denotes WT
T8956 44996-45000 JJ denotes null
T8957 44985-44988 CC denotes and
T8958 44989-44992 NN denotes TGF
T8959 44993-44995 NN denotes β2
T8960 44992-44993 HYPH denotes -
T8961 45001-45005 NNS denotes buds
T8962 45016-45023 JJ denotes nuclear
T8963 45044-45056 NN denotes localization
T8964 45024-45027 NN denotes LEF
T8965 45027-45028 HYPH denotes -
T8966 45028-45029 CD denotes 1
T8967 45030-45033 CC denotes and
T8968 45034-45035 NN denotes β
T8969 45036-45043 NN denotes catenin
T8970 45035-45036 HYPH denotes -
T8971 45056-45058 , denotes ,
T8972 45058-45063 NNS denotes signs
T8973 45064-45068 IN denotes that
T8974 45102-45105 VBD denotes was
T8975 45069-45072 DT denotes the
T8976 45094-45101 NN denotes pathway
T8977 45073-45076 NN denotes Wnt
T8978 45077-45083 NN denotes noggin
T8979 45076-45077 HYPH denotes -
T8980 45084-45093 NN denotes signaling
T8981 45106-45112 JJ denotes intact
T9019 45308-45310 , denotes ,
T9020 45310-45313 NN denotes TGF
T9021 45314-45316 NN denotes β2
T9022 45313-45314 HYPH denotes -
T9023 45325-45327 TO denotes to
T9024 45328-45332 VB denotes work
T9025 45333-45335 IN denotes in
T9026 45336-45342 NN denotes tandem
T9027 45343-45347 IN denotes with
T9028 45348-45353 DT denotes these
T9029 45360-45370 NNS denotes morphogens
T9030 45354-45359 JJ denotes other
T9031 45371-45373 TO denotes to
T9032 45379-45387 VB denotes regulate
T9033 45374-45378 RB denotes down
T9034 45378-45379 HYPH denotes -
T9035 45388-45389 NN denotes E
T9036 45390-45398 NN denotes cadherin
T9037 45389-45390 HYPH denotes -
T9038 45399-45405 NNS denotes levels
T9039 45405-45407 , denotes ,
T9040 45407-45412 WDT denotes which
T9041 45413-45424 VBZ denotes contributes
T9042 45425-45427 IN denotes to
T9043 45428-45431 DT denotes the
T9044 45432-45442 NN denotes activation
T9045 45443-45445 IN denotes of
T9046 45446-45459 JJ denotes proliferative
T9047 45460-45471 NNS denotes circuitries
T9048 45471-45472 . denotes .
T9197 46100-46102 , denotes ,
T9102 45485-45491 IN denotes During
T9103 45582-45588 VBP denotes govern
T9104 45492-45499 NN denotes budding
T9105 45500-45513 NN denotes morphogenesis
T9106 45513-45515 , denotes ,
T9107 45515-45527 VBG denotes intersecting
T9108 45538-45546 NNS denotes networks
T9109 45528-45537 NN denotes signaling
T9110 45547-45551 IN denotes from
T9111 45552-45555 DT denotes the
T9112 45556-45566 NN denotes epithelium
T9113 45567-45570 CC denotes and
T9114 45571-45581 NN denotes mesenchyme
T9115 45589-45604 JJ denotes transcriptional
T9116 45639-45647 NNS denotes programs
T9117 45604-45606 , denotes ,
T9118 45606-45614 JJ denotes adhesive
T9119 45614-45616 , denotes ,
T9120 45616-45624 NN denotes polarity
T9121 45624-45626 , denotes ,
T9122 45626-45629 CC denotes and
T9123 45630-45638 NN denotes motility
T9124 45648-45650 IN denotes in
T9125 45651-45656 DT denotes these
T9126 45664-45670 NNS denotes groups
T9127 45657-45663 JJ denotes select
T9128 45671-45673 IN denotes of
T9129 45674-45679 NNS denotes cells
T9130 45679-45680 . denotes .
T9131 45680-45801 sentence denotes The dynamic nuclear and cytosolic changes that take place during this time form the cornerstone for organ morphogenesis.
T9132 45681-45684 DT denotes The
T9133 45715-45722 NNS denotes changes
T9134 45685-45692 JJ denotes dynamic
T9135 45693-45700 JJ denotes nuclear
T9136 45701-45704 CC denotes and
T9137 45705-45714 JJ denotes cytosolic
T9138 45756-45760 VBP denotes form
T9139 45723-45727 WDT denotes that
T9140 45728-45732 VBP denotes take
T9141 45733-45738 NN denotes place
T9142 45739-45745 IN denotes during
T9143 45746-45750 DT denotes this
T9144 45751-45755 NN denotes time
T9145 45761-45764 DT denotes the
T9146 45765-45776 NN denotes cornerstone
T9147 45777-45780 IN denotes for
T9148 45781-45786 NN denotes organ
T9149 45787-45800 NN denotes morphogenesis
T9150 45800-45801 . denotes .
T9151 45801-46122 sentence denotes Two major challenges in understanding the mechanisms underlying a particular budding process are to order the temporal sequence of external cues involved and then to dissect how the cells of the developing bud translate these signals into the downstream events of cellular remodeling, proliferation, and differentiation.
T9152 45802-45805 CD denotes Two
T9153 45812-45822 NNS denotes challenges
T9154 45806-45811 JJ denotes major
T9155 45895-45898 VBP denotes are
T9156 45823-45825 IN denotes in
T9157 45826-45839 VBG denotes understanding
T9158 45840-45843 DT denotes the
T9159 45844-45854 NNS denotes mechanisms
T9160 45855-45865 VBG denotes underlying
T9161 45866-45867 DT denotes a
T9162 45887-45894 NN denotes process
T9163 45868-45878 JJ denotes particular
T9164 45879-45886 NN denotes budding
T9165 45899-45901 TO denotes to
T9166 45902-45907 VB denotes order
T9167 45908-45911 DT denotes the
T9168 45921-45929 NN denotes sequence
T9169 45912-45920 JJ denotes temporal
T9170 45930-45932 IN denotes of
T9171 45933-45941 JJ denotes external
T9172 45942-45946 NNS denotes cues
T9173 45947-45955 VBN denotes involved
T9174 45956-45959 CC denotes and
T9175 45960-45964 RB denotes then
T9176 45968-45975 VB denotes dissect
T9177 45965-45967 TO denotes to
T9178 45976-45979 WRB denotes how
T9179 46012-46021 VB denotes translate
T9180 45980-45983 DT denotes the
T9181 45984-45989 NNS denotes cells
T9182 45990-45992 IN denotes of
T9183 45993-45996 DT denotes the
T9184 46008-46011 NN denotes bud
T9185 45997-46007 VBG denotes developing
T9186 46022-46027 DT denotes these
T9187 46028-46035 NNS denotes signals
T9188 46036-46040 IN denotes into
T9189 46041-46044 DT denotes the
T9190 46056-46062 NNS denotes events
T9191 46045-46055 JJ denotes downstream
T9192 46063-46065 IN denotes of
T9193 46066-46074 JJ denotes cellular
T9194 46075-46085 NN denotes remodeling
T9195 46085-46087 , denotes ,
T9196 46087-46100 NN denotes proliferation
T9198 46102-46105 CC denotes and
T9199 46106-46121 NN denotes differentiation
T9200 46121-46122 . denotes .
T9201 46122-46246 sentence denotes Our studies here provide some insights into how these events are orchestrated during hair bud formation in developing skin.
T9202 46123-46126 PRP$ denotes Our
T9203 46127-46134 NNS denotes studies
T9204 46140-46147 VBP denotes provide
T9205 46135-46139 RB denotes here
T9206 46148-46152 DT denotes some
T9207 46153-46161 NNS denotes insights
T9208 46162-46166 IN denotes into
T9209 46167-46170 WRB denotes how
T9210 46188-46200 VBN denotes orchestrated
T9211 46171-46176 DT denotes these
T9212 46177-46183 NNS denotes events
T9213 46184-46187 VBP denotes are
T9214 46201-46207 IN denotes during
T9215 46208-46212 NN denotes hair
T9216 46213-46216 NN denotes bud
T9217 46217-46226 NN denotes formation
T9218 46227-46229 IN denotes in
T9219 46230-46240 VBG denotes developing
T9220 46241-46245 NN denotes skin
T9221 46245-46246 . denotes .
T9653 46248-46257 NN denotes Signaling
T9654 46258-46264 IN denotes during
T9655 46265-46270 JJ denotes Early
T9656 46285-46298 NN denotes Morphogenesis
T9657 46271-46275 NN denotes Hair
T9658 46276-46284 NN denotes Follicle
T9659 46298-46624 sentence denotes Recent studies on hair bud morphogenesis suggest that Wnt signals likely from the epithelium and BMP inhibitory signals from the underlying mesenchyme converge to produce an active transcription factor complex involving β-catenin and LEF-1, which in turn plays a key role in specifying the hair follicle fate [4,29,30,36,37].
T9660 46299-46305 JJ denotes Recent
T9661 46306-46313 NNS denotes studies
T9662 46340-46347 VBP denotes suggest
T9663 46314-46316 IN denotes on
T9664 46317-46321 NN denotes hair
T9665 46322-46325 NN denotes bud
T9666 46326-46339 NN denotes morphogenesis
T9667 46348-46352 IN denotes that
T9668 46450-46458 VBP denotes converge
T9669 46353-46356 NN denotes Wnt
T9670 46357-46364 NNS denotes signals
T9671 46365-46371 RB denotes likely
T9672 46372-46376 IN denotes from
T9673 46377-46380 DT denotes the
T9674 46381-46391 NN denotes epithelium
T9675 46392-46395 CC denotes and
T9676 46396-46399 NN denotes BMP
T9677 46411-46418 NNS denotes signals
T9678 46400-46410 JJ denotes inhibitory
T9679 46419-46423 IN denotes from
T9680 46424-46427 DT denotes the
T9681 46439-46449 NN denotes mesenchyme
T9682 46428-46438 VBG denotes underlying
T9683 46459-46461 TO denotes to
T9684 46462-46469 VB denotes produce
T9685 46470-46472 DT denotes an
T9686 46501-46508 NN denotes complex
T9687 46473-46479 JJ denotes active
T9688 46480-46493 NN denotes transcription
T9689 46494-46500 NN denotes factor
T9690 46509-46518 VBG denotes involving
T9691 46519-46520 NN denotes β
T9692 46521-46528 NN denotes catenin
T9693 46520-46521 HYPH denotes -
T9694 46529-46532 CC denotes and
T9695 46533-46536 NN denotes LEF
T9696 46536-46537 HYPH denotes -
T9697 46537-46538 CD denotes 1
T9698 46538-46540 , denotes ,
T9699 46540-46545 WDT denotes which
T9700 46554-46559 VBZ denotes plays
T9701 46546-46548 IN denotes in
T9702 46549-46553 NN denotes turn
T9703 46560-46561 DT denotes a
T9704 46566-46570 NN denotes role
T9705 46562-46565 JJ denotes key
T9706 46571-46573 IN denotes in
T9707 46574-46584 VBG denotes specifying
T9708 46585-46588 DT denotes the
T9709 46603-46607 NN denotes fate
T9710 46589-46593 NN denotes hair
T9711 46594-46602 NN denotes follicle
T9712 46608-46609 -LRB- denotes [
T9713 46620-46622 CD denotes 37
T9714 46609-46610 CD denotes 4
T9715 46610-46611 , denotes ,
T9716 46611-46613 CD denotes 29
T9717 46613-46614 , denotes ,
T9718 46614-46616 CD denotes 30
T9719 46616-46617 , denotes ,
T9720 46617-46619 CD denotes 36
T9721 46619-46620 , denotes ,
T9722 46622-46623 -RRB- denotes ]
T9723 46623-46624 . denotes .
T9724 46624-46883 sentence denotes Sonic hedgehog (Shh) and TGF-β2 signaling also play essential roles in follicle morphogenesis, but in contrast to β-catenin null skin, in which follicle invaginations are absent [30], some hair buds still form in the absence of LEF-1, Shh, or TGF-β2 [32,38].
T9725 46625-46630 JJ denotes Sonic
T9726 46631-46639 NN denotes hedgehog
T9727 46672-46676 VBP denotes play
T9728 46640-46641 -LRB- denotes (
T9729 46641-46644 NN denotes Shh
T9730 46644-46645 -RRB- denotes )
T9731 46646-46649 CC denotes and
T9732 46650-46653 NN denotes TGF
T9733 46654-46656 NN denotes β2
T9734 46653-46654 HYPH denotes -
T9735 46657-46666 NN denotes signaling
T9736 46667-46671 RB denotes also
T9737 46677-46686 JJ denotes essential
T9738 46687-46692 NNS denotes roles
T9739 46693-46695 IN denotes in
T9740 46696-46704 NN denotes follicle
T9741 46705-46718 NN denotes morphogenesis
T9742 46718-46720 , denotes ,
T9743 46720-46723 CC denotes but
T9744 46724-46726 IN denotes in
T9745 46830-46834 VBP denotes form
T9746 46727-46735 NN denotes contrast
T9747 46736-46738 IN denotes to
T9748 46739-46740 NN denotes β
T9749 46741-46748 NN denotes catenin
T9750 46740-46741 HYPH denotes -
T9751 46749-46753 JJ denotes null
T9752 46754-46758 NN denotes skin
T9753 46758-46760 , denotes ,
T9754 46760-46762 IN denotes in
T9755 46792-46795 VBP denotes are
T9756 46763-46768 WDT denotes which
T9757 46769-46777 NN denotes follicle
T9758 46778-46791 NNS denotes invaginations
T9759 46796-46802 JJ denotes absent
T9760 46803-46804 -LRB- denotes [
T9761 46804-46806 CD denotes 30
T9762 46806-46807 -RRB- denotes ]
T9763 46807-46809 , denotes ,
T9764 46809-46813 DT denotes some
T9765 46819-46823 NNS denotes buds
T9766 46814-46818 NN denotes hair
T9767 46824-46829 RB denotes still
T9768 46835-46837 IN denotes in
T9769 46838-46841 DT denotes the
T9770 46842-46849 NN denotes absence
T9771 46850-46852 IN denotes of
T9772 46853-46856 NN denotes LEF
T9773 46856-46857 HYPH denotes -
T9774 46857-46858 CD denotes 1
T9775 46858-46860 , denotes ,
T9776 46860-46863 NN denotes Shh
T9777 46863-46865 , denotes ,
T9778 46865-46867 CC denotes or
T9779 46868-46871 NN denotes TGF
T9780 46872-46874 NN denotes β2
T9781 46871-46872 HYPH denotes -
T9782 46875-46876 -LRB- denotes [
T9783 46879-46881 CD denotes 38
T9784 46876-46878 CD denotes 32
T9785 46878-46879 , denotes ,
T9786 46881-46882 -RRB- denotes ]
T9787 46882-46883 . denotes .
T9788 46883-47066 sentence denotes These likely reflect the first wave of follicle (i.e., guard hair) morphogenesis, which accounts for a small number (fewer than 5%) of hairs and is under distinct regulatory control.
T9789 46884-46889 DT denotes These
T9790 46897-46904 VBP denotes reflect
T9791 46890-46896 RB denotes likely
T9792 46905-46908 DT denotes the
T9793 46915-46919 NN denotes wave
T9794 46909-46914 JJ denotes first
T9795 46920-46922 IN denotes of
T9796 46923-46931 NN denotes follicle
T9797 46951-46964 NN denotes morphogenesis
T9798 46932-46933 -LRB- denotes (
T9799 46945-46949 NN denotes hair
T9800 46933-46937 FW denotes i.e.
T9801 46937-46939 , denotes ,
T9802 46939-46944 NN denotes guard
T9803 46949-46950 -RRB- denotes )
T9804 46964-46966 , denotes ,
T9805 46966-46971 WDT denotes which
T9806 46972-46980 VBZ denotes accounts
T9807 46981-46984 IN denotes for
T9808 46985-46986 DT denotes a
T9809 46993-46999 NN denotes number
T9810 46987-46992 JJ denotes small
T9811 47000-47001 -LRB- denotes (
T9812 47001-47006 JJR denotes fewer
T9813 47012-47013 CD denotes 5
T9814 47007-47011 IN denotes than
T9815 47013-47014 NN denotes %
T9816 47014-47015 -RRB- denotes )
T9817 47016-47018 IN denotes of
T9818 47019-47024 NNS denotes hairs
T9819 47025-47028 CC denotes and
T9820 47029-47031 VBZ denotes is
T9821 47032-47037 IN denotes under
T9822 47038-47046 JJ denotes distinct
T9823 47058-47065 NN denotes control
T9824 47047-47057 JJ denotes regulatory
T9825 47065-47066 . denotes .
T9826 47066-47230 sentence denotes Guard hairs form in the absence of LEF-1 and TGF-β2, and we have found that they also fail to express Snail at the budding stage of development (unpublished data).
T9827 47067-47072 NN denotes Guard
T9828 47073-47078 NNS denotes hairs
T9829 47079-47083 VBP denotes form
T9830 47084-47086 IN denotes in
T9831 47087-47090 DT denotes the
T9832 47091-47098 NN denotes absence
T9833 47099-47101 IN denotes of
T9834 47102-47105 NN denotes LEF
T9835 47105-47106 HYPH denotes -
T9836 47106-47107 CD denotes 1
T9837 47108-47111 CC denotes and
T9838 47112-47115 NN denotes TGF
T9839 47116-47118 NN denotes β2
T9840 47115-47116 HYPH denotes -
T9841 47118-47120 , denotes ,
T9842 47120-47123 CC denotes and
T9843 47124-47126 PRP denotes we
T9844 47132-47137 VBN denotes found
T9845 47127-47131 VBP denotes have
T9846 47138-47142 IN denotes that
T9847 47153-47157 VBP denotes fail
T9848 47143-47147 PRP denotes they
T9849 47148-47152 RB denotes also
T9850 47158-47160 TO denotes to
T9851 47161-47168 VB denotes express
T9852 47169-47174 NN denotes Snail
T9853 47175-47177 IN denotes at
T9854 47178-47181 DT denotes the
T9855 47190-47195 NN denotes stage
T9856 47182-47189 NN denotes budding
T9857 47196-47198 IN denotes of
T9858 47199-47210 NN denotes development
T9859 47211-47212 -LRB- denotes (
T9860 47224-47228 NNS denotes data
T9861 47212-47223 JJ denotes unpublished
T9862 47228-47229 -RRB- denotes )
T9863 47229-47230 . denotes .
T9864 47230-47299 sentence denotes How E-cadherin is regulated in guard hairs remains to be determined.
T9865 47231-47234 WRB denotes How
T9866 47249-47258 VBN denotes regulated
T9867 47235-47236 NN denotes E
T9868 47237-47245 NN denotes cadherin
T9869 47236-47237 HYPH denotes -
T9870 47246-47248 VBZ denotes is
T9871 47274-47281 VBZ denotes remains
T9872 47259-47261 IN denotes in
T9873 47262-47267 NN denotes guard
T9874 47268-47273 NNS denotes hairs
T9875 47282-47284 TO denotes to
T9876 47288-47298 VBN denotes determined
T9877 47285-47287 VB denotes be
T9878 47298-47299 . denotes .
T9879 47299-47533 sentence denotes Several candidates include other Snail family members such as Slug or twist, or alternatively, transcription factors involving β-catenin and a different member of the LEF-1/TCF/Sry-type HMG box (commonly known as SOX) family [39,40].
T9880 47300-47307 JJ denotes Several
T9881 47308-47318 NNS denotes candidates
T9882 47319-47326 VBP denotes include
T9883 47327-47332 JJ denotes other
T9884 47346-47353 NNS denotes members
T9885 47333-47338 NN denotes Snail
T9886 47339-47345 NN denotes family
T9887 47354-47358 JJ denotes such
T9888 47359-47361 IN denotes as
T9889 47362-47366 NN denotes Slug
T9890 47367-47369 CC denotes or
T9891 47370-47375 NN denotes twist
T9892 47375-47377 , denotes ,
T9893 47377-47379 CC denotes or
T9894 47380-47393 RB denotes alternatively
T9895 47409-47416 NNS denotes factors
T9896 47393-47395 , denotes ,
T9897 47395-47408 NN denotes transcription
T9898 47417-47426 VBG denotes involving
T9899 47427-47428 NN denotes β
T9900 47429-47436 NN denotes catenin
T9901 47428-47429 HYPH denotes -
T9902 47437-47440 CC denotes and
T9903 47441-47442 DT denotes a
T9904 47453-47459 NN denotes member
T9905 47443-47452 JJ denotes different
T9906 47460-47462 IN denotes of
T9907 47463-47466 DT denotes the
T9908 47518-47524 NN denotes family
T9909 47467-47470 NN denotes LEF
T9910 47490-47493 NN denotes box
T9911 47470-47471 HYPH denotes -
T9912 47471-47472 CD denotes 1
T9913 47472-47473 HYPH denotes /
T9914 47473-47476 NN denotes TCF
T9915 47476-47477 HYPH denotes /
T9916 47477-47480 NN denotes Sry
T9917 47481-47485 NN denotes type
T9918 47480-47481 HYPH denotes -
T9919 47486-47489 NN denotes HMG
T9920 47494-47495 -LRB- denotes (
T9921 47504-47509 VBN denotes known
T9922 47495-47503 RB denotes commonly
T9923 47510-47512 IN denotes as
T9924 47513-47516 NN denotes SOX
T9925 47516-47517 -RRB- denotes )
T9926 47525-47526 -LRB- denotes [
T9927 47529-47531 CD denotes 40
T9928 47526-47528 CD denotes 39
T9929 47528-47529 , denotes ,
T9930 47531-47532 -RRB- denotes ]
T9931 47532-47533 . denotes .
T9932 47533-47676 sentence denotes Further investigation will be required to determine whether the signaling pathway we have elucidated here is a theme with multiple variations.
T9933 47534-47541 JJ denotes Further
T9934 47542-47555 NN denotes investigation
T9935 47564-47572 VBN denotes required
T9936 47556-47560 MD denotes will
T9937 47561-47563 VB denotes be
T9938 47573-47575 TO denotes to
T9939 47576-47585 VB denotes determine
T9940 47586-47593 IN denotes whether
T9941 47640-47642 VBZ denotes is
T9942 47594-47597 DT denotes the
T9943 47608-47615 NN denotes pathway
T9944 47598-47607 NN denotes signaling
T9945 47616-47618 PRP denotes we
T9946 47624-47634 VBN denotes elucidated
T9947 47619-47623 VBP denotes have
T9948 47635-47639 RB denotes here
T9949 47643-47644 DT denotes a
T9950 47645-47650 NN denotes theme
T9951 47651-47655 IN denotes with
T9952 47656-47664 JJ denotes multiple
T9953 47665-47675 NNS denotes variations
T9954 47675-47676 . denotes .
T9955 47676-47758 sentence denotes TGF-βs are known to promote withdrawal of keratinocytes from the cell cycle [41].
T9956 47677-47680 NN denotes TGF
T9957 47681-47683 NNS denotes βs
T9958 47680-47681 HYPH denotes -
T9959 47688-47693 VBN denotes known
T9960 47684-47687 VBP denotes are
T9961 47694-47696 TO denotes to
T9963 47705-47715 NN denotes withdrawal
T9964 47716-47718 IN denotes of
T9965 47719-47732 NNS denotes keratinocytes
T9966 47733-47737 IN denotes from
T9967 47738-47741 DT denotes the
T9968 47747-47752 NN denotes cycle
T9969 47742-47746 NN denotes cell
T9970 47753-47754 -LRB- denotes [
T9971 47754-47756 CD denotes 41
T9972 47756-47757 -RRB- denotes ]
T9973 47757-47758 . denotes .
T9974 47758-48040 sentence denotes Hence, when TGF-β2 protein was detected at the transition between the growing and destructive phases of the adult hair cycle, research initially and naturally focused on a role for this family member in cessation of growth and/or triggering apoptosis ([42] and references therein).
T9975 47759-47764 RB denotes Hence
T9976 47918-47925 VBD denotes focused
T9977 47764-47766 , denotes ,
T9978 47766-47770 WRB denotes when
T9979 47790-47798 VBN denotes detected
T9980 47771-47774 NN denotes TGF
T9981 47775-47777 NN denotes β2
T9982 47774-47775 HYPH denotes -
T9983 47778-47785 NN denotes protein
T9984 47786-47789 VBD denotes was
T9985 47799-47801 IN denotes at
T9986 47802-47805 DT denotes the
T9987 47806-47816 NN denotes transition
T9988 47817-47824 IN denotes between
T9989 47825-47828 DT denotes the
T9990 47853-47859 NNS denotes phases
T9991 47829-47836 NN denotes growing
T9992 47841-47852 JJ denotes destructive
T9993 47837-47840 CC denotes and
T9994 47860-47862 IN denotes of
T9995 47863-47866 DT denotes the
T9996 47878-47883 NN denotes cycle
T9997 47867-47872 JJ denotes adult
T9998 47873-47877 NN denotes hair
T9999 47883-47885 , denotes ,
T10000 47885-47893 NN denotes research
T10001 47894-47903 RB denotes initially
T10002 47904-47907 CC denotes and
T10003 47908-47917 RB denotes naturally
T10004 47926-47928 IN denotes on
T10005 47929-47930 DT denotes a
T10006 47931-47935 NN denotes role
T10007 47936-47939 IN denotes for
T10008 47940-47944 DT denotes this
T10009 47952-47958 NN denotes member
T10010 47945-47951 NN denotes family
T10011 47959-47961 IN denotes in
T10012 47962-47971 NN denotes cessation
T10013 47972-47974 IN denotes of
T10014 47975-47981 NN denotes growth
T10015 47982-47985 CC denotes and
T10016 47985-47986 HYPH denotes /
T10017 47986-47988 CC denotes or
T10018 47989-47999 VBG denotes triggering
T10019 48000-48009 NN denotes apoptosis
T10020 48010-48011 -LRB- denotes (
T10021 48012-48014 CD denotes 42
T10022 48011-48012 -LRB- denotes [
T10023 48014-48015 -RRB- denotes ]
T10024 48016-48019 CC denotes and
T10025 48020-48030 NNS denotes references
T10026 48031-48038 RB denotes therein
T10027 48038-48039 -RRB- denotes )
T10028 48039-48040 . denotes .
T10029 48040-48231 sentence denotes However, in contrast to TGF-β1-null skin, which exhibits an extended growing phase of postnatal hair follicles, TGF-β2-null skin displays an embryonic block in follicle bud progression [32].
T10030 48041-48048 RB denotes However
T10031 48170-48178 VBZ denotes displays
T10032 48048-48050 , denotes ,
T10033 48050-48052 IN denotes in
T10034 48053-48061 NN denotes contrast
T10035 48062-48064 IN denotes to
T10036 48065-48068 NN denotes TGF
T10037 48069-48071 NN denotes β1
T10038 48068-48069 HYPH denotes -
T10039 48072-48076 JJ denotes null
T10040 48071-48072 HYPH denotes -
T10041 48077-48081 NN denotes skin
T10042 48081-48083 , denotes ,
T10043 48083-48088 WDT denotes which
T10044 48089-48097 VBZ denotes exhibits
T10045 48098-48100 DT denotes an
T10046 48118-48123 NN denotes phase
T10047 48101-48109 JJ denotes extended
T10048 48110-48117 NN denotes growing
T10049 48124-48126 IN denotes of
T10050 48127-48136 JJ denotes postnatal
T10051 48142-48151 NNS denotes follicles
T10052 48137-48141 NN denotes hair
T10053 48151-48153 , denotes ,
T10054 48153-48156 NN denotes TGF
T10055 48157-48159 NN denotes β2
T10056 48156-48157 HYPH denotes -
T10057 48160-48164 JJ denotes null
T10058 48159-48160 HYPH denotes -
T10059 48165-48169 NN denotes skin
T10060 48179-48181 DT denotes an
T10061 48192-48197 NN denotes block
T10062 48182-48191 JJ denotes embryonic
T10063 48198-48200 IN denotes in
T10064 48201-48209 NN denotes follicle
T10065 48210-48213 NN denotes bud
T10066 48214-48225 NN denotes progression
T10067 48226-48227 -LRB- denotes [
T10068 48227-48229 CD denotes 32
T10069 48229-48230 -RRB- denotes ]
T10070 48230-48231 . denotes .
T10071 48231-48502 sentence denotes Although this phenotype is consistent with TGF-β2's embryonic expression patterns [33], about 50% of TGF-β2 null buds appear unable to progress to the down-growth phase, a feature that cannot be explained readily on the basis of previously established effects of TGF-βs.
T10072 48232-48240 IN denotes Although
T10073 48256-48258 VBZ denotes is
T10074 48241-48245 DT denotes this
T10075 48246-48255 NN denotes phenotype
T10076 48350-48356 VBP denotes appear
T10077 48259-48269 JJ denotes consistent
T10078 48270-48274 IN denotes with
T10079 48275-48278 NN denotes TGF
T10080 48279-48281 NN denotes β2
T10081 48278-48279 HYPH denotes -
T10082 48305-48313 NNS denotes patterns
T10083 48281-48283 POS denotes 's
T10084 48284-48293 JJ denotes embryonic
T10085 48294-48304 NN denotes expression
T10086 48314-48315 -LRB- denotes [
T10087 48315-48317 CD denotes 33
T10088 48317-48318 -RRB- denotes ]
T10089 48318-48320 , denotes ,
T10090 48320-48325 IN denotes about
T10091 48326-48328 CD denotes 50
T10092 48328-48329 NN denotes %
T10093 48330-48332 IN denotes of
T10094 48333-48336 NN denotes TGF
T10095 48337-48339 NN denotes β2
T10096 48336-48337 HYPH denotes -
T10097 48340-48344 JJ denotes null
T10098 48345-48349 NNS denotes buds
T10099 48357-48363 JJ denotes unable
T10100 48364-48366 TO denotes to
T10101 48367-48375 VB denotes progress
T10102 48376-48378 IN denotes to
T10103 48379-48382 DT denotes the
T10104 48395-48400 NN denotes phase
T10105 48383-48387 JJ denotes down
T10106 48388-48394 NN denotes growth
T10107 48387-48388 HYPH denotes -
T10108 48400-48402 , denotes ,
T10109 48402-48403 DT denotes a
T10110 48404-48411 NN denotes feature
T10111 48412-48416 WDT denotes that
T10112 48427-48436 VBN denotes explained
T10113 48417-48420 MD denotes can
T10114 48420-48423 RB denotes not
T10115 48424-48426 VB denotes be
T10116 48437-48444 RB denotes readily
T10117 48445-48447 IN denotes on
T10118 48448-48451 DT denotes the
T10119 48452-48457 NN denotes basis
T10120 48458-48460 IN denotes of
T10121 48461-48471 RB denotes previously
T10122 48472-48483 VBN denotes established
T10123 48484-48491 NNS denotes effects
T10124 48492-48494 IN denotes of
T10125 48495-48498 NN denotes TGF
T10126 48499-48501 NNS denotes βs
T10127 48498-48499 HYPH denotes -
T10128 48501-48502 . denotes .
T10129 48502-48777 sentence denotes Our finding that TGF-β2 is upstream from Ki67 expression and MAPK activation lends further support to the notion that hair follicle keratinocytes at this early stage of development react to TGF-β2 signaling in a fashion opposite to that typically expected for TGF-β factors.
T10130 48503-48506 PRP$ denotes Our
T10131 48507-48514 NN denotes finding
T10132 48580-48585 VBZ denotes lends
T10133 48515-48519 IN denotes that
T10134 48527-48529 VBZ denotes is
T10135 48520-48523 NN denotes TGF
T10136 48524-48526 NN denotes β2
T10137 48523-48524 HYPH denotes -
T10138 48530-48538 RB denotes upstream
T10139 48539-48543 IN denotes from
T10140 48544-48548 NN denotes Ki67
T10141 48549-48559 NN denotes expression
T10142 48560-48563 CC denotes and
T10143 48564-48568 NN denotes MAPK
T10144 48569-48579 NN denotes activation
T10145 48586-48593 JJ denotes further
T10146 48594-48601 NN denotes support
T10147 48602-48604 IN denotes to
T10148 48605-48608 DT denotes the
T10149 48609-48615 NN denotes notion
T10150 48616-48620 IN denotes that
T10151 48684-48689 VBP denotes react
T10152 48621-48625 NN denotes hair
T10153 48626-48634 NN denotes follicle
T10154 48635-48648 NNS denotes keratinocytes
T10155 48649-48651 IN denotes at
T10156 48652-48656 DT denotes this
T10157 48663-48668 NN denotes stage
T10158 48657-48662 JJ denotes early
T10159 48669-48671 IN denotes of
T10160 48672-48683 NN denotes development
T10161 48690-48692 IN denotes to
T10162 48693-48696 NN denotes TGF
T10163 48697-48699 NN denotes β2
T10164 48696-48697 HYPH denotes -
T10165 48700-48709 NN denotes signaling
T10166 48710-48712 IN denotes in
T10167 48713-48714 DT denotes a
T10168 48715-48722 NN denotes fashion
T10169 48723-48731 JJ denotes opposite
T10170 48732-48734 IN denotes to
T10171 48735-48739 DT denotes that
T10172 48740-48749 RB denotes typically
T10173 48750-48758 VBN denotes expected
T10174 48759-48762 IN denotes for
T10175 48763-48766 NN denotes TGF
T10176 48767-48768 NN denotes β
T10177 48766-48767 HYPH denotes -
T10178 48769-48776 NNS denotes factors
T10179 48776-48777 . denotes .
T10180 48777-48895 sentence denotes This said, based upon pSMAD2 immunohistochemistry, the immediate steps of downstream signaling appeared to be intact.
T10181 48778-48782 DT denotes This
T10182 48783-48787 VBN denotes said
T10183 48873-48881 VBD denotes appeared
T10184 48787-48789 , denotes ,
T10185 48789-48794 VBN denotes based
T10186 48795-48799 IN denotes upon
T10187 48800-48806 NN denotes pSMAD2
T10188 48807-48827 NN denotes immunohistochemistry
T10189 48827-48829 , denotes ,
T10190 48829-48832 DT denotes the
T10191 48843-48848 NNS denotes steps
T10192 48833-48842 JJ denotes immediate
T10193 48849-48851 IN denotes of
T10194 48852-48862 JJ denotes downstream
T10195 48863-48872 NN denotes signaling
T10196 48882-48884 TO denotes to
T10197 48885-48887 VB denotes be
T10198 48888-48894 JJ denotes intact
T10199 48894-48895 . denotes .
T10200 48895-49031 sentence denotes Thus, we surmise that the proliferative outcome is likely to be rooted in differences in the repertoire of activated SMAD target genes.
T10201 48896-48900 RB denotes Thus
T10202 48905-48912 VBP denotes surmise
T10203 48900-48902 , denotes ,
T10204 48902-48904 PRP denotes we
T10205 48913-48917 IN denotes that
T10206 48944-48946 VBZ denotes is
T10207 48918-48921 DT denotes the
T10208 48936-48943 NN denotes outcome
T10209 48922-48935 JJ denotes proliferative
T10210 48947-48953 JJ denotes likely
T10211 48954-48956 TO denotes to
T10212 48960-48966 VBN denotes rooted
T10213 48957-48959 VB denotes be
T10214 48967-48969 IN denotes in
T10215 48970-48981 NNS denotes differences
T10216 48982-48984 IN denotes in
T10217 48985-48988 DT denotes the
T10218 48989-48999 NN denotes repertoire
T10219 49000-49002 IN denotes of
T10220 49003-49012 VBN denotes activated
T10221 49025-49030 NNS denotes genes
T10222 49013-49017 NN denotes SMAD
T10223 49018-49024 NN denotes target
T10224 49030-49031 . denotes .
T10225 49031-49296 sentence denotes In this regard, the positive effects of TGF-β2 on proliferation within the hair bud may be more analogous to what has been seen in progression of squamous cell carcinoma to metastatic carcinoma [43] rather than that typically observed for keratinocytes [44,45,46].
T10226 49032-49034 IN denotes In
T10227 49120-49122 VB denotes be
T10228 49035-49039 DT denotes this
T10229 49040-49046 NN denotes regard
T10230 49046-49048 , denotes ,
T10231 49048-49051 DT denotes the
T10232 49061-49068 NNS denotes effects
T10233 49052-49060 JJ denotes positive
T10234 49069-49071 IN denotes of
T10235 49072-49075 NN denotes TGF
T10236 49076-49078 NN denotes β2
T10237 49075-49076 HYPH denotes -
T10238 49079-49081 IN denotes on
T10239 49082-49095 NN denotes proliferation
T10240 49096-49102 IN denotes within
T10241 49103-49106 DT denotes the
T10242 49112-49115 NN denotes bud
T10243 49107-49111 NN denotes hair
T10244 49116-49119 MD denotes may
T10245 49123-49127 RBR denotes more
T10246 49128-49137 JJ denotes analogous
T10247 49138-49140 IN denotes to
T10248 49141-49145 WP denotes what
T10249 49155-49159 VBN denotes seen
T10250 49146-49149 VBZ denotes has
T10251 49150-49154 VBN denotes been
T10252 49160-49162 IN denotes in
T10253 49163-49174 NN denotes progression
T10254 49175-49177 IN denotes of
T10255 49178-49186 JJ denotes squamous
T10256 49187-49191 NN denotes cell
T10257 49192-49201 NN denotes carcinoma
T10258 49202-49204 IN denotes to
T10259 49205-49215 JJ denotes metastatic
T10260 49216-49225 NN denotes carcinoma
T10261 49226-49227 -LRB- denotes [
T10262 49227-49229 CD denotes 43
T10263 49229-49230 -RRB- denotes ]
T10264 49231-49237 RB denotes rather
T10265 49238-49242 IN denotes than
T10266 49243-49247 DT denotes that
T10267 49248-49257 RB denotes typically
T10268 49258-49266 VBN denotes observed
T10269 49267-49270 IN denotes for
T10270 49271-49284 NNS denotes keratinocytes
T10271 49285-49286 -LRB- denotes [
T10272 49292-49294 CD denotes 46
T10273 49286-49288 CD denotes 44
T10274 49288-49289 , denotes ,
T10275 49289-49291 CD denotes 45
T10276 49291-49292 , denotes ,
T10277 49294-49295 -RRB- denotes ]
T10278 49295-49296 . denotes .
T10279 49296-49490 sentence denotes The prior identification of the Snail gene as a potential target of TGF-β signaling [15] was intriguing, given the temporal wave of Snail gene expression that occurs in the developing hair bud.
T10280 49297-49300 DT denotes The
T10281 49307-49321 NN denotes identification
T10282 49301-49306 JJ denotes prior
T10283 49386-49389 VBD denotes was
T10284 49322-49324 IN denotes of
T10285 49325-49328 DT denotes the
T10286 49335-49339 NN denotes gene
T10287 49329-49334 NN denotes Snail
T10288 49340-49342 IN denotes as
T10289 49343-49344 DT denotes a
T10290 49355-49361 NN denotes target
T10291 49345-49354 JJ denotes potential
T10292 49362-49364 IN denotes of
T10293 49365-49368 NN denotes TGF
T10294 49369-49370 NN denotes β
T10295 49368-49369 HYPH denotes -
T10296 49371-49380 NN denotes signaling
T10297 49381-49382 -LRB- denotes [
T10298 49382-49384 CD denotes 15
T10299 49384-49385 -RRB- denotes ]
T10300 49390-49400 JJ denotes intriguing
T10301 49400-49402 , denotes ,
T10302 49402-49407 VBN denotes given
T10303 49408-49411 DT denotes the
T10304 49421-49425 NN denotes wave
T10305 49412-49420 JJ denotes temporal
T10306 49426-49428 IN denotes of
T10307 49429-49434 NN denotes Snail
T10308 49440-49450 NN denotes expression
T10309 49435-49439 NN denotes gene
T10310 49451-49455 WDT denotes that
T10311 49456-49462 VBZ denotes occurs
T10312 49463-49465 IN denotes in
T10313 49466-49469 DT denotes the
T10314 49486-49489 NN denotes bud
T10315 49470-49480 VBG denotes developing
T10316 49481-49485 NN denotes hair
T10317 49489-49490 . denotes .
T10318 49490-49661 sentence denotes The additional correlation between epithelial hyperproliferation and Snail transgene expression further fostered our interest in a possible link between TGF-β2 and Snail.
T10319 49491-49494 DT denotes The
T10320 49506-49517 NN denotes correlation
T10321 49495-49505 JJ denotes additional
T10322 49595-49603 VBD denotes fostered
T10323 49518-49525 IN denotes between
T10324 49526-49536 JJ denotes epithelial
T10325 49537-49555 NN denotes hyperproliferation
T10326 49556-49559 CC denotes and
T10327 49560-49565 NN denotes Snail
T10328 49576-49586 NN denotes expression
T10329 49566-49575 NN denotes transgene
T10330 49587-49594 RB denotes further
T10331 49604-49607 PRP$ denotes our
T10332 49608-49616 NN denotes interest
T10333 49617-49619 IN denotes in
T10334 49620-49621 DT denotes a
T10335 49631-49635 NN denotes link
T10336 49622-49630 JJ denotes possible
T10337 49636-49643 IN denotes between
T10338 49644-49647 NN denotes TGF
T10339 49648-49650 NN denotes β2
T10340 49647-49648 HYPH denotes -
T10341 49651-49654 CC denotes and
T10342 49655-49660 NN denotes Snail
T10343 49660-49661 . denotes .
T10344 49661-49839 sentence denotes Our functional studies demonstrate that without TGF-β2, Snail expression is abolished in the mutant hair buds, and conversely, in K14-Smad2 skin, Snail is ectopically activated.
T10345 49662-49665 PRP$ denotes Our
T10346 49677-49684 NNS denotes studies
T10347 49666-49676 JJ denotes functional
T10348 49685-49696 VBP denotes demonstrate
T10349 49697-49701 IN denotes that
T10350 49738-49747 VBN denotes abolished
T10351 49702-49709 IN denotes without
T10352 49710-49713 NN denotes TGF
T10353 49714-49716 NN denotes β2
T10354 49713-49714 HYPH denotes -
T10355 49716-49718 , denotes ,
T10356 49718-49723 NN denotes Snail
T10357 49724-49734 NN denotes expression
T10358 49735-49737 VBZ denotes is
T10359 49748-49750 IN denotes in
T10360 49751-49754 DT denotes the
T10361 49767-49771 NNS denotes buds
T10362 49755-49761 NN denotes mutant
T10363 49762-49766 NN denotes hair
T10364 49771-49773 , denotes ,
T10365 49773-49776 CC denotes and
T10366 49777-49787 RB denotes conversely
T10367 49829-49838 VBN denotes activated
T10368 49787-49789 , denotes ,
T10369 49789-49791 IN denotes in
T10370 49792-49795 NN denotes K14
T10371 49796-49801 NN denotes Smad2
T10372 49795-49796 HYPH denotes -
T10373 49802-49806 NN denotes skin
T10374 49806-49808 , denotes ,
T10375 49808-49813 NN denotes Snail
T10376 49814-49816 VBZ denotes is
T10377 49817-49828 RB denotes ectopically
T10378 49838-49839 . denotes .
T10379 49839-50070 sentence denotes Moreover, our in vitro studies indicate that even sustained TGF-β2 exposure may cause only a transient induction of Snail, offering a possible explanation as to why Snail is so briefly expressed during hair follicle morphogenesis.
T10380 49840-49848 RB denotes Moreover
T10381 49871-49879 VBP denotes indicate
T10382 49848-49850 , denotes ,
T10383 49850-49853 PRP$ denotes our
T10384 49863-49870 NNS denotes studies
T10385 49854-49856 FW denotes in
T10386 49857-49862 FW denotes vitro
T10387 49880-49884 IN denotes that
T10388 49920-49925 VB denotes cause
T10389 49885-49889 RB denotes even
T10390 49907-49915 NN denotes exposure
T10391 49890-49899 JJ denotes sustained
T10392 49900-49903 NN denotes TGF
T10393 49904-49906 NN denotes β2
T10394 49903-49904 HYPH denotes -
T10395 49916-49919 MD denotes may
T10396 49926-49930 RB denotes only
T10397 49943-49952 NN denotes induction
T10398 49931-49932 DT denotes a
T10399 49933-49942 JJ denotes transient
T10400 49953-49955 IN denotes of
T10401 49956-49961 NN denotes Snail
T10402 49961-49963 , denotes ,
T10403 49963-49971 VBG denotes offering
T10404 49972-49973 DT denotes a
T10405 49983-49994 NN denotes explanation
T10406 49974-49982 JJ denotes possible
T10407 49995-49997 IN denotes as
T10408 49998-50000 IN denotes to
T10409 50001-50004 WRB denotes why
T10410 50025-50034 VBN denotes expressed
T10411 50005-50010 NN denotes Snail
T10412 50011-50013 VBZ denotes is
T10413 50014-50016 RB denotes so
T10414 50017-50024 RB denotes briefly
T10415 50035-50041 IN denotes during
T10416 50042-50046 NN denotes hair
T10417 50047-50055 NN denotes follicle
T10418 50056-50069 NN denotes morphogenesis
T10419 50069-50070 . denotes .
T10420 50070-50387 sentence denotes An additional point worth mentioning is that prolonged expression of Tg Snail in postnatal skin resulted in morphological and biochemical signs of epithelial to mesenchymal transitions (unpublished data), underscoring why transient Snail expression may be so important during normal hair follicle morphogenesis [18].
T10421 50071-50073 DT denotes An
T10422 50085-50090 NN denotes point
T10423 50074-50084 JJ denotes additional
T10424 50108-50110 VBZ denotes is
T10425 50091-50096 JJ denotes worth
T10426 50097-50107 VBG denotes mentioning
T10427 50111-50115 IN denotes that
T10428 50167-50175 VBD denotes resulted
T10429 50116-50125 VBN denotes prolonged
T10430 50126-50136 NN denotes expression
T10431 50137-50139 IN denotes of
T10432 50140-50142 NN denotes Tg
T10433 50143-50148 NN denotes Snail
T10434 50149-50151 IN denotes in
T10435 50152-50161 JJ denotes postnatal
T10436 50162-50166 NN denotes skin
T10437 50176-50178 IN denotes in
T10438 50179-50192 JJ denotes morphological
T10439 50209-50214 NNS denotes signs
T10440 50193-50196 CC denotes and
T10441 50197-50208 JJ denotes biochemical
T10442 50215-50217 IN denotes of
T10443 50218-50228 JJ denotes epithelial
T10444 50244-50255 NNS denotes transitions
T10445 50229-50231 IN denotes to
T10446 50232-50243 JJ denotes mesenchymal
T10447 50256-50257 -LRB- denotes (
T10448 50269-50273 NNS denotes data
T10449 50257-50268 JJ denotes unpublished
T10450 50273-50274 -RRB- denotes )
T10451 50274-50276 , denotes ,
T10452 50276-50288 VBG denotes underscoring
T10453 50289-50292 WRB denotes why
T10454 50324-50326 VB denotes be
T10455 50293-50302 JJ denotes transient
T10456 50309-50319 NN denotes expression
T10457 50303-50308 NN denotes Snail
T10458 50320-50323 MD denotes may
T10459 50327-50329 RB denotes so
T10460 50330-50339 JJ denotes important
T10461 50340-50346 IN denotes during
T10462 50347-50353 JJ denotes normal
T10463 50368-50381 NN denotes morphogenesis
T10464 50354-50358 NN denotes hair
T10465 50359-50367 NN denotes follicle
T10466 50382-50383 -LRB- denotes [
T10467 50383-50385 CD denotes 18
T10468 50385-50386 -RRB- denotes ]
T10469 50386-50387 . denotes .
T10470 50387-50520 sentence denotes At first glance, the sparsity in hair coat of K14-Snail Tg mice seemed indicative of a defect in follicle formation (see Figure 2A).
T10471 50388-50390 IN denotes At
T10472 50452-50458 VBD denotes seemed
T10473 50391-50396 JJ denotes first
T10474 50397-50403 NN denotes glance
T10475 50403-50405 , denotes ,
T10476 50405-50408 DT denotes the
T10477 50409-50417 NN denotes sparsity
T10478 50418-50420 IN denotes in
T10479 50421-50425 NN denotes hair
T10480 50426-50430 NN denotes coat
T10481 50431-50433 IN denotes of
T10482 50434-50437 NN denotes K14
T10483 50438-50443 NN denotes Snail
T10484 50437-50438 HYPH denotes -
T10485 50447-50451 NNS denotes mice
T10486 50444-50446 NN denotes Tg
T10487 50459-50469 JJ denotes indicative
T10488 50470-50472 IN denotes of
T10489 50473-50474 DT denotes a
T10490 50475-50481 NN denotes defect
T10491 50482-50484 IN denotes in
T10492 50485-50493 NN denotes follicle
T10493 50494-50503 NN denotes formation
T10494 50504-50505 -LRB- denotes (
T10495 50505-50508 VB denotes see
T10496 50509-50515 NN denotes Figure
T10497 50516-50518 NN denotes 2A
T10498 50518-50519 -RRB- denotes )
T10499 50519-50520 . denotes .
T10500 50520-50620 sentence denotes Closer inspection, however, revealed that not all hairs penetrated the hyperthickened Tg epidermis.
T10501 50521-50527 JJR denotes Closer
T10502 50528-50538 NN denotes inspection
T10503 50549-50557 VBD denotes revealed
T10504 50538-50540 , denotes ,
T10505 50540-50547 RB denotes however
T10506 50547-50549 , denotes ,
T10507 50558-50562 IN denotes that
T10508 50577-50587 VBD denotes penetrated
T10509 50563-50566 RB denotes not
T10510 50571-50576 NNS denotes hairs
T10511 50567-50570 DT denotes all
T10512 50588-50591 DT denotes the
T10513 50610-50619 NN denotes epidermis
T10514 50592-50606 VBN denotes hyperthickened
T10515 50607-50609 NN denotes Tg
T10516 50619-50620 . denotes .
T10517 50620-50711 sentence denotes Several factors may contribute to the seemingly normal follicle development in these mice.
T10518 50621-50628 JJ denotes Several
T10519 50629-50636 NNS denotes factors
T10520 50641-50651 VB denotes contribute
T10521 50637-50640 MD denotes may
T10522 50652-50654 IN denotes to
T10523 50655-50658 DT denotes the
T10524 50685-50696 NN denotes development
T10525 50659-50668 RB denotes seemingly
T10526 50669-50675 JJ denotes normal
T10527 50676-50684 NN denotes follicle
T10528 50697-50699 IN denotes in
T10529 50700-50705 DT denotes these
T10530 50706-50710 NNS denotes mice
T10531 50710-50711 . denotes .
T10532 50711-50975 sentence denotes One obvious factor is the K14 promoter, which is elevated in the basal layer of the epidermis and the outer root sheath (ORS) of the hair follicle, but is markedly down-regulated in developing embryonic hair buds as well as in the postnatal hair progenitor cells.
T10533 50712-50715 CD denotes One
T10534 50724-50730 NN denotes factor
T10535 50716-50723 JJ denotes obvious
T10536 50731-50733 VBZ denotes is
T10537 50734-50737 DT denotes the
T10538 50742-50750 NN denotes promoter
T10539 50738-50741 NN denotes K14
T10540 50750-50752 , denotes ,
T10541 50752-50757 WDT denotes which
T10542 50758-50760 VBZ denotes is
T10543 50761-50769 JJ denotes elevated
T10544 50770-50772 IN denotes in
T10545 50773-50776 DT denotes the
T10546 50783-50788 NN denotes layer
T10547 50777-50782 JJ denotes basal
T10548 50789-50791 IN denotes of
T10549 50792-50795 DT denotes the
T10550 50796-50805 NN denotes epidermis
T10551 50806-50809 CC denotes and
T10552 50810-50813 DT denotes the
T10553 50825-50831 NN denotes sheath
T10554 50814-50819 JJ denotes outer
T10555 50820-50824 NN denotes root
T10556 50832-50833 -LRB- denotes (
T10557 50833-50836 NN denotes ORS
T10558 50836-50837 -RRB- denotes )
T10559 50838-50840 IN denotes of
T10560 50841-50844 DT denotes the
T10561 50850-50858 NN denotes follicle
T10562 50845-50849 NN denotes hair
T10563 50858-50860 , denotes ,
T10564 50860-50863 CC denotes but
T10565 50864-50866 VBZ denotes is
T10566 50881-50890 VBN denotes regulated
T10567 50867-50875 RB denotes markedly
T10568 50876-50880 RB denotes down
T10569 50880-50881 HYPH denotes -
T10570 50891-50893 IN denotes in
T10571 50894-50904 JJ denotes developing
T10572 50920-50924 NNS denotes buds
T10573 50905-50914 JJ denotes embryonic
T10574 50915-50919 NN denotes hair
T10575 50925-50927 RB denotes as
T10576 50933-50935 IN denotes as
T10577 50928-50932 RB denotes well
T10578 50936-50938 IN denotes in
T10579 50939-50942 DT denotes the
T10580 50969-50974 NNS denotes cells
T10581 50943-50952 JJ denotes postnatal
T10582 50953-50957 NN denotes hair
T10583 50958-50968 NN denotes progenitor
T10584 50974-50975 . denotes .
T10585 50975-51135 sentence denotes The K14 promoter is also less active in the ORS than epidermis and hence this might also account for the lack of apparent response of the ORS to ectopic Snail.
T10586 50976-50979 DT denotes The
T10587 50984-50992 NN denotes promoter
T10588 50980-50983 NN denotes K14
T10589 50993-50995 VBZ denotes is
T10590 50996-51000 RB denotes also
T10591 51001-51005 RBR denotes less
T10592 51006-51012 JJ denotes active
T10593 51013-51015 IN denotes in
T10594 51016-51019 DT denotes the
T10595 51020-51023 NN denotes ORS
T10596 51024-51028 IN denotes than
T10597 51029-51038 NN denotes epidermis
T10598 51039-51042 CC denotes and
T10599 51043-51048 RB denotes hence
T10600 51065-51072 VB denotes account
T10601 51049-51053 DT denotes this
T10602 51054-51059 MD denotes might
T10603 51060-51064 RB denotes also
T10604 51073-51076 IN denotes for
T10605 51077-51080 DT denotes the
T10606 51081-51085 NN denotes lack
T10607 51086-51088 IN denotes of
T10608 51089-51097 JJ denotes apparent
T10609 51098-51106 NN denotes response
T10610 51107-51109 IN denotes of
T10611 51110-51113 DT denotes the
T10612 51114-51117 NN denotes ORS
T10613 51118-51120 IN denotes to
T10614 51121-51128 JJ denotes ectopic
T10615 51129-51134 NN denotes Snail
T10616 51134-51135 . denotes .
T10617 51135-51386 sentence denotes Additional contributing factors could be the multiplicity of Snail family members and their differential expression, the saturation, and/or diversity of regulatory mechanisms that govern AJ formation, migration, and proliferation in the follicle ORS.
T10618 51136-51146 JJ denotes Additional
T10619 51160-51167 NNS denotes factors
T10620 51147-51159 VBG denotes contributing
T10621 51174-51176 VB denotes be
T10622 51168-51173 MD denotes could
T10623 51177-51180 DT denotes the
T10624 51181-51193 NN denotes multiplicity
T10625 51194-51196 IN denotes of
T10626 51197-51202 NN denotes Snail
T10627 51210-51217 NNS denotes members
T10628 51203-51209 NN denotes family
T10629 51218-51221 CC denotes and
T10630 51222-51227 PRP$ denotes their
T10631 51241-51251 NN denotes expression
T10632 51228-51240 JJ denotes differential
T10633 51251-51253 , denotes ,
T10634 51253-51256 DT denotes the
T10635 51257-51267 NN denotes saturation
T10636 51267-51269 , denotes ,
T10637 51269-51272 CC denotes and
T10638 51272-51273 HYPH denotes /
T10639 51273-51275 CC denotes or
T10640 51276-51285 NN denotes diversity
T10641 51286-51288 IN denotes of
T10642 51289-51299 JJ denotes regulatory
T10643 51300-51310 NNS denotes mechanisms
T10644 51311-51315 WDT denotes that
T10645 51316-51322 VBP denotes govern
T10646 51323-51325 NN denotes AJ
T10647 51326-51335 NN denotes formation
T10648 51335-51337 , denotes ,
T10649 51337-51346 NN denotes migration
T10650 51346-51348 , denotes ,
T10651 51348-51351 CC denotes and
T10652 51352-51365 NN denotes proliferation
T10653 51366-51368 IN denotes in
T10654 51369-51372 DT denotes the
T10655 51382-51385 NN denotes ORS
T10656 51373-51381 NN denotes follicle
T10657 51385-51386 . denotes .
T10658 51386-51521 sentence denotes Distinguishing between these possibilities must await the generation of mice harboring skin-specific loss-of-function Snail mutations.
T10659 51387-51401 VBG denotes Distinguishing
T10660 51435-51440 VB denotes await
T10661 51402-51409 IN denotes between
T10662 51410-51415 DT denotes these
T10663 51416-51429 NNS denotes possibilities
T10664 51430-51434 MD denotes must
T10665 51441-51444 DT denotes the
T10666 51445-51455 NN denotes generation
T10667 51456-51458 IN denotes of
T10668 51459-51463 NNS denotes mice
T10669 51464-51473 VBG denotes harboring
T10670 51474-51478 NN denotes skin
T10671 51479-51487 JJ denotes specific
T10672 51478-51479 HYPH denotes -
T10673 51511-51520 NNS denotes mutations
T10674 51488-51492 NN denotes loss
T10675 51492-51493 HYPH denotes -
T10676 51493-51495 IN denotes of
T10677 51495-51496 HYPH denotes -
T10678 51496-51504 NN denotes function
T10679 51505-51510 NN denotes Snail
T10680 51520-51521 . denotes .
T11057 51523-51528 NNS denotes Links
T11058 51529-51536 IN denotes between
T11059 51537-51546 NN denotes Signaling
T11060 51546-51548 , denotes ,
T11061 51548-51563 JJ denotes Transcriptional
T11062 51564-51572 NNS denotes Cascades
T11063 51572-51574 , denotes ,
T11064 51574-51584 JJ denotes Epithelial
T11065 51585-51595 NN denotes Remodeling
T11066 51595-51597 , denotes ,
T11067 51597-51600 CC denotes and
T11068 51601-51614 NN denotes Proliferation
T11069 51615-51617 IN denotes in
T11070 51618-51621 DT denotes the
T11071 51627-51630 NN denotes Bud
T11072 51622-51626 NN denotes Hair
T11073 51630-51827 sentence denotes Previously, we discovered that early during hair follicle morphogenesis, E-cadherin gene expression is down-regulated concomitantly with the invagination of developing bud cells into the skin [4].
T11074 51631-51641 RB denotes Previously
T11075 51646-51656 VBD denotes discovered
T11076 51641-51643 , denotes ,
T11077 51643-51645 PRP denotes we
T11078 51657-51661 IN denotes that
T11079 51739-51748 VBN denotes regulated
T11080 51662-51667 RB denotes early
T11081 51668-51674 IN denotes during
T11082 51675-51679 NN denotes hair
T11083 51680-51688 NN denotes follicle
T11084 51689-51702 NN denotes morphogenesis
T11085 51702-51704 , denotes ,
T11086 51704-51705 NN denotes E
T11087 51706-51714 NN denotes cadherin
T11088 51705-51706 HYPH denotes -
T11089 51720-51730 NN denotes expression
T11090 51715-51719 NN denotes gene
T11091 51731-51733 VBZ denotes is
T11092 51734-51738 RB denotes down
T11093 51738-51739 HYPH denotes -
T11094 51749-51762 RB denotes concomitantly
T11095 51763-51767 IN denotes with
T11096 51768-51771 DT denotes the
T11097 51772-51784 NN denotes invagination
T11098 51785-51787 IN denotes of
T11099 51788-51798 JJ denotes developing
T11100 51803-51808 NNS denotes cells
T11101 51799-51802 NN denotes bud
T11102 51809-51813 IN denotes into
T11103 51814-51817 DT denotes the
T11104 51818-51822 NN denotes skin
T11105 51823-51824 -LRB- denotes [
T11106 51824-51825 CD denotes 4
T11107 51825-51826 -RRB- denotes ]
T11108 51826-51827 . denotes .
T11109 51827-52046 sentence denotes Because the timing of this event correlated with the activation of a LEF-1/β-catenin transcription factor complex [20], we were intrigued by the presence of a putative LEF-1/TCF binding site in the E-cadherin promoter.
T11110 51828-51835 IN denotes Because
T11111 51861-51871 VBD denotes correlated
T11112 51836-51839 DT denotes the
T11113 51840-51846 NN denotes timing
T11114 51847-51849 IN denotes of
T11115 51850-51854 DT denotes this
T11116 51855-51860 NN denotes event
T11117 51956-51965 VBN denotes intrigued
T11118 51872-51876 IN denotes with
T11119 51877-51880 DT denotes the
T11120 51881-51891 NN denotes activation
T11121 51892-51894 IN denotes of
T11122 51895-51896 DT denotes a
T11123 51934-51941 NN denotes complex
T11124 51897-51900 NN denotes LEF
T11125 51927-51933 NN denotes factor
T11126 51900-51901 HYPH denotes -
T11127 51901-51902 CD denotes 1
T11128 51902-51903 HYPH denotes /
T11129 51903-51904 NN denotes β
T11130 51905-51912 NN denotes catenin
T11131 51904-51905 HYPH denotes -
T11132 51913-51926 NN denotes transcription
T11133 51942-51943 -LRB- denotes [
T11134 51943-51945 CD denotes 20
T11135 51945-51946 -RRB- denotes ]
T11136 51946-51948 , denotes ,
T11137 51948-51950 PRP denotes we
T11138 51951-51955 VBD denotes were
T11139 51966-51968 IN denotes by
T11140 51969-51972 DT denotes the
T11141 51973-51981 NN denotes presence
T11142 51982-51984 IN denotes of
T11143 51985-51986 DT denotes a
T11144 52014-52018 NN denotes site
T11145 51987-51995 JJ denotes putative
T11146 51996-51999 NN denotes LEF
T11147 51999-52000 HYPH denotes -
T11148 52000-52001 CD denotes 1
T11149 52001-52002 HYPH denotes /
T11150 52002-52005 NN denotes TCF
T11151 52006-52013 NN denotes binding
T11152 52019-52021 IN denotes in
T11153 52022-52025 DT denotes the
T11154 52037-52045 NN denotes promoter
T11155 52026-52027 NN denotes E
T11156 52028-52036 NN denotes cadherin
T11157 52027-52028 HYPH denotes -
T11158 52045-52046 . denotes .
T11159 52046-52223 sentence denotes This prompted an investigation that subsequently led to our discovery that LEF-1/β-catenin can contribute to repression of E-cadherin gene expression in skin keratinocytes [4].
T11160 52047-52051 DT denotes This
T11161 52052-52060 VBD denotes prompted
T11162 52061-52063 DT denotes an
T11163 52064-52077 NN denotes investigation
T11164 52078-52082 WDT denotes that
T11165 52096-52099 VBD denotes led
T11166 52083-52095 RB denotes subsequently
T11167 52100-52102 IN denotes to
T11168 52103-52106 PRP$ denotes our
T11169 52107-52116 NN denotes discovery
T11170 52117-52121 IN denotes that
T11171 52142-52152 VB denotes contribute
T11172 52122-52125 NN denotes LEF
T11173 52125-52126 HYPH denotes -
T11174 52126-52127 CD denotes 1
T11175 52127-52128 HYPH denotes /
T11176 52128-52129 NN denotes β
T11177 52130-52137 NN denotes catenin
T11178 52129-52130 HYPH denotes -
T11179 52138-52141 MD denotes can
T11180 52153-52155 IN denotes to
T11181 52156-52166 NN denotes repression
T11182 52167-52169 IN denotes of
T11183 52170-52171 NN denotes E
T11184 52172-52180 NN denotes cadherin
T11185 52171-52172 HYPH denotes -
T11186 52186-52196 NN denotes expression
T11187 52181-52185 NN denotes gene
T11188 52197-52199 IN denotes in
T11189 52200-52204 NN denotes skin
T11190 52205-52218 NNS denotes keratinocytes
T11191 52219-52220 -LRB- denotes [
T11192 52220-52221 CD denotes 4
T11193 52221-52222 -RRB- denotes ]
T11194 52222-52223 . denotes .
T11195 52223-52475 sentence denotes In the course of these studies, we also noted that Snail can also contribute to this process in keratinocytes in vitro, and our present studies revealed that Snail is expressed at the right place and time to be physiologically relevant in the process.
T11196 52224-52226 IN denotes In
T11197 52264-52269 VBD denotes noted
T11198 52227-52230 DT denotes the
T11199 52231-52237 NN denotes course
T11200 52238-52240 IN denotes of
T11201 52241-52246 DT denotes these
T11202 52247-52254 NNS denotes studies
T11203 52254-52256 , denotes ,
T11204 52256-52258 PRP denotes we
T11205 52259-52263 RB denotes also
T11206 52270-52274 IN denotes that
T11207 52290-52300 VB denotes contribute
T11208 52275-52280 NN denotes Snail
T11209 52281-52284 MD denotes can
T11210 52285-52289 RB denotes also
T11211 52301-52303 IN denotes to
T11212 52304-52308 DT denotes this
T11213 52309-52316 NN denotes process
T11214 52317-52319 IN denotes in
T11215 52320-52333 NNS denotes keratinocytes
T11216 52334-52336 FW denotes in
T11217 52337-52342 FW denotes vitro
T11218 52342-52344 , denotes ,
T11219 52344-52347 CC denotes and
T11220 52348-52351 PRP$ denotes our
T11221 52360-52367 NNS denotes studies
T11222 52352-52359 JJ denotes present
T11223 52368-52376 VBD denotes revealed
T11224 52377-52381 IN denotes that
T11225 52391-52400 VBN denotes expressed
T11226 52382-52387 NN denotes Snail
T11227 52388-52390 VBZ denotes is
T11228 52401-52403 IN denotes at
T11229 52404-52407 DT denotes the
T11230 52414-52419 NN denotes place
T11231 52408-52413 JJ denotes right
T11232 52420-52423 CC denotes and
T11233 52424-52428 NN denotes time
T11234 52429-52431 TO denotes to
T11235 52432-52434 VB denotes be
T11236 52435-52450 RB denotes physiologically
T11237 52451-52459 JJ denotes relevant
T11238 52460-52462 IN denotes in
T11239 52463-52466 DT denotes the
T11240 52467-52474 NN denotes process
T11241 52474-52475 . denotes .
T11242 52475-52627 sentence denotes In noggin-null embryonic skin, LEF-1 expression and subsequent activation of the LEF-1/β-catenin reporter gene is abrogated in the developing placodes.
T11243 52476-52478 IN denotes In
T11244 52590-52599 VBN denotes abrogated
T11245 52479-52485 NN denotes noggin
T11246 52486-52490 JJ denotes null
T11247 52485-52486 HYPH denotes -
T11248 52501-52505 NN denotes skin
T11249 52491-52500 JJ denotes embryonic
T11250 52505-52507 , denotes ,
T11251 52507-52510 NN denotes LEF
T11252 52513-52523 NN denotes expression
T11253 52510-52511 HYPH denotes -
T11254 52511-52512 CD denotes 1
T11255 52524-52527 CC denotes and
T11256 52528-52538 JJ denotes subsequent
T11257 52539-52549 NN denotes activation
T11258 52550-52552 IN denotes of
T11259 52553-52556 DT denotes the
T11260 52582-52586 NN denotes gene
T11261 52557-52560 NN denotes LEF
T11262 52560-52561 HYPH denotes -
T11263 52561-52562 CD denotes 1
T11264 52562-52563 HYPH denotes /
T11265 52563-52564 NN denotes β
T11266 52565-52572 NN denotes catenin
T11267 52564-52565 HYPH denotes -
T11268 52573-52581 NN denotes reporter
T11269 52587-52589 VBZ denotes is
T11270 52600-52602 IN denotes in
T11271 52603-52606 DT denotes the
T11272 52618-52626 NNS denotes placodes
T11273 52607-52617 VBG denotes developing
T11274 52626-52627 . denotes .
T11275 52627-52790 sentence denotes The corresponding failure of E-cadherin down-regulation underscores the importance of Wnt/noggin signaling in regulating this event in follicle morphogenesis [4].
T11276 52628-52631 DT denotes The
T11277 52646-52653 NN denotes failure
T11278 52632-52645 VBG denotes corresponding
T11279 52684-52695 VBZ denotes underscores
T11280 52654-52656 IN denotes of
T11281 52657-52658 NN denotes E
T11282 52659-52667 NN denotes cadherin
T11283 52658-52659 HYPH denotes -
T11284 52673-52683 NN denotes regulation
T11285 52668-52672 JJ denotes down
T11286 52672-52673 HYPH denotes -
T11287 52696-52699 DT denotes the
T11288 52700-52710 NN denotes importance
T11289 52711-52713 IN denotes of
T11290 52714-52717 NN denotes Wnt
T11291 52718-52724 NN denotes noggin
T11292 52717-52718 HYPH denotes /
T11293 52725-52734 NN denotes signaling
T11294 52735-52737 IN denotes in
T11295 52738-52748 VBG denotes regulating
T11296 52749-52753 DT denotes this
T11297 52754-52759 NN denotes event
T11298 52760-52762 IN denotes in
T11299 52763-52771 NN denotes follicle
T11300 52772-52785 NN denotes morphogenesis
T11301 52786-52787 -LRB- denotes [
T11302 52787-52788 CD denotes 4
T11303 52788-52789 -RRB- denotes ]
T11304 52789-52790 . denotes .
T11305 52790-52993 sentence denotes Conditional gene targeting studies will be necessary to establish whether Snail family members also contribute to the down-regulation in E-cadherin gene expression that occurs during follicle formation.
T11306 52791-52802 JJ denotes Conditional
T11307 52818-52825 NNS denotes studies
T11308 52803-52807 NN denotes gene
T11309 52808-52817 NN denotes targeting
T11310 52831-52833 VB denotes be
T11311 52826-52830 MD denotes will
T11312 52834-52843 JJ denotes necessary
T11313 52844-52846 TO denotes to
T11314 52847-52856 VB denotes establish
T11315 52857-52864 IN denotes whether
T11316 52891-52901 VBP denotes contribute
T11317 52865-52870 NN denotes Snail
T11318 52878-52885 NNS denotes members
T11319 52871-52877 NN denotes family
T11320 52886-52890 RB denotes also
T11321 52902-52904 IN denotes to
T11322 52905-52908 DT denotes the
T11323 52914-52924 NN denotes regulation
T11324 52909-52913 JJ denotes down
T11325 52913-52914 HYPH denotes -
T11326 52925-52927 IN denotes in
T11327 52928-52929 NN denotes E
T11328 52930-52938 NN denotes cadherin
T11329 52929-52930 HYPH denotes -
T11330 52944-52954 NN denotes expression
T11331 52939-52943 NN denotes gene
T11332 52955-52959 WDT denotes that
T11333 52960-52966 VBZ denotes occurs
T11334 52967-52973 IN denotes during
T11335 52974-52982 NN denotes follicle
T11336 52983-52992 NN denotes formation
T11337 52992-52993 . denotes .
T11338 52993-53162 sentence denotes However, it is intriguing that K14-Snail Tg epidermis displayed a marked down-regulation in E-cadherin expression, thereby demonstrating its potential to do so in skin.
T11339 52994-53001 RB denotes However
T11340 53006-53008 VBZ denotes is
T11341 53001-53003 , denotes ,
T11342 53003-53005 PRP denotes it
T11343 53009-53019 VBG denotes intriguing
T11344 53020-53024 IN denotes that
T11345 53048-53057 VBD denotes displayed
T11346 53025-53028 NN denotes K14
T11347 53029-53034 NN denotes Snail
T11348 53028-53029 HYPH denotes -
T11349 53038-53047 NN denotes epidermis
T11350 53035-53037 NN denotes Tg
T11351 53058-53059 DT denotes a
T11352 53072-53082 NN denotes regulation
T11353 53060-53066 JJ denotes marked
T11354 53067-53071 JJ denotes down
T11355 53071-53072 HYPH denotes -
T11356 53083-53085 IN denotes in
T11357 53086-53087 NN denotes E
T11358 53088-53096 NN denotes cadherin
T11359 53087-53088 HYPH denotes -
T11360 53097-53107 NN denotes expression
T11361 53107-53109 , denotes ,
T11362 53109-53116 RB denotes thereby
T11363 53117-53130 VBG denotes demonstrating
T11364 53131-53134 PRP$ denotes its
T11365 53135-53144 NN denotes potential
T11366 53145-53147 TO denotes to
T11367 53148-53150 VB denotes do
T11368 53151-53153 RB denotes so
T11369 53154-53156 IN denotes in
T11370 53157-53161 NN denotes skin
T11371 53161-53162 . denotes .
T11372 53162-53315 sentence denotes Our prior findings showed that by elevating E-cadherin levels or by conditionally ablating α-catenin, hair follicle morphogenesis can be impaired [4,7].
T11373 53163-53166 PRP$ denotes Our
T11374 53173-53181 NNS denotes findings
T11375 53167-53172 JJ denotes prior
T11376 53182-53188 VBD denotes showed
T11377 53189-53193 IN denotes that
T11378 53300-53308 VBN denotes impaired
T11379 53194-53196 IN denotes by
T11380 53197-53206 VBG denotes elevating
T11381 53207-53208 NN denotes E
T11382 53209-53217 NN denotes cadherin
T11383 53208-53209 HYPH denotes -
T11384 53218-53224 NNS denotes levels
T11385 53225-53227 CC denotes or
T11386 53228-53230 IN denotes by
T11387 53231-53244 RB denotes conditionally
T11388 53245-53253 VBG denotes ablating
T11389 53254-53255 NN denotes α
T11390 53256-53263 NN denotes catenin
T11391 53255-53256 HYPH denotes -
T11392 53263-53265 , denotes ,
T11393 53265-53269 NN denotes hair
T11394 53270-53278 NN denotes follicle
T11395 53279-53292 NN denotes morphogenesis
T11396 53293-53296 MD denotes can
T11397 53297-53299 VB denotes be
T11398 53309-53310 -LRB- denotes [
T11399 53312-53313 CD denotes 7
T11400 53310-53311 CD denotes 4
T11401 53311-53312 , denotes ,
T11402 53313-53314 -RRB- denotes ]
T11403 53314-53315 . denotes .
T11404 53315-53645 sentence denotes The marked epidermal hyperproliferation seen in the K14-Snail Tg skin, coupled with the converse suppression of proliferation and Snail in TGF-β2-null hair buds led us to wonder whether the down-regulation of E-cadherin during follicle morphogenesis might have a direct impact on elevating the proliferative state of these cells.
T11405 53316-53319 DT denotes The
T11406 53337-53355 NN denotes hyperproliferation
T11407 53320-53326 JJ denotes marked
T11408 53327-53336 JJ denotes epidermal
T11409 53477-53480 VBD denotes led
T11410 53356-53360 VBN denotes seen
T11411 53361-53363 IN denotes in
T11412 53364-53367 DT denotes the
T11413 53381-53385 NN denotes skin
T11414 53368-53371 NN denotes K14
T11415 53372-53377 NN denotes Snail
T11416 53371-53372 HYPH denotes -
T11417 53378-53380 NN denotes Tg
T11418 53385-53387 , denotes ,
T11419 53387-53394 VBN denotes coupled
T11420 53395-53399 IN denotes with
T11421 53400-53403 DT denotes the
T11422 53413-53424 NN denotes suppression
T11423 53404-53412 JJ denotes converse
T11424 53425-53427 IN denotes of
T11425 53428-53441 NN denotes proliferation
T11426 53442-53445 CC denotes and
T11427 53446-53451 NN denotes Snail
T11428 53452-53454 IN denotes in
T11429 53455-53458 NN denotes TGF
T11430 53459-53461 NN denotes β2
T11431 53458-53459 HYPH denotes -
T11432 53462-53466 JJ denotes null
T11433 53461-53462 HYPH denotes -
T11434 53472-53476 NNS denotes buds
T11435 53467-53471 NN denotes hair
T11436 53481-53483 PRP denotes us
T11437 53484-53486 TO denotes to
T11438 53487-53493 VB denotes wonder
T11439 53494-53501 IN denotes whether
T11440 53572-53576 VB denotes have
T11441 53502-53505 DT denotes the
T11442 53511-53521 NN denotes regulation
T11443 53506-53510 JJ denotes down
T11444 53510-53511 HYPH denotes -
T11445 53522-53524 IN denotes of
T11446 53525-53526 NN denotes E
T11447 53527-53535 NN denotes cadherin
T11448 53526-53527 HYPH denotes -
T11449 53536-53542 IN denotes during
T11450 53543-53551 NN denotes follicle
T11451 53552-53565 NN denotes morphogenesis
T11452 53566-53571 MD denotes might
T11453 53577-53578 DT denotes a
T11454 53586-53592 NN denotes impact
T11455 53579-53585 JJ denotes direct
T11456 53593-53595 IN denotes on
T11457 53596-53605 VBG denotes elevating
T11458 53606-53609 DT denotes the
T11459 53624-53629 NN denotes state
T11460 53610-53623 JJ denotes proliferative
T11461 53630-53632 IN denotes of
T11462 53633-53638 DT denotes these
T11463 53639-53644 NNS denotes cells
T11464 53644-53645 . denotes .
T11465 53645-53827 sentence denotes Our Tg studies suggested that, at least in part through its regulation of E-cadherin, Snail is able to influence the subcellular localization of a variety of AJ-associated proteins.
T11466 53646-53649 PRP$ denotes Our
T11467 53653-53660 NNS denotes studies
T11468 53650-53652 NN denotes Tg
T11469 53661-53670 VBD denotes suggested
T11470 53671-53675 IN denotes that
T11471 53738-53740 VBZ denotes is
T11472 53675-53677 , denotes ,
T11473 53677-53679 RB denotes at
T11474 53680-53685 RBS denotes least
T11475 53686-53688 IN denotes in
T11476 53694-53701 IN denotes through
T11477 53689-53693 NN denotes part
T11478 53702-53705 PRP$ denotes its
T11479 53706-53716 NN denotes regulation
T11480 53717-53719 IN denotes of
T11481 53720-53721 NN denotes E
T11482 53722-53730 NN denotes cadherin
T11483 53721-53722 HYPH denotes -
T11484 53730-53732 , denotes ,
T11485 53732-53737 NN denotes Snail
T11486 53741-53745 JJ denotes able
T11487 53746-53748 TO denotes to
T11488 53749-53758 VB denotes influence
T11489 53759-53762 DT denotes the
T11490 53775-53787 NN denotes localization
T11491 53763-53774 JJ denotes subcellular
T11492 53788-53790 IN denotes of
T11493 53791-53792 DT denotes a
T11494 53793-53800 NN denotes variety
T11495 53801-53803 IN denotes of
T11496 53804-53806 NN denotes AJ
T11497 53807-53817 VBN denotes associated
T11498 53806-53807 HYPH denotes -
T11499 53818-53826 NN denotes proteins
T11500 53826-53827 . denotes .
T11501 53827-53957 sentence denotes One of these appears to be Ajuba, which was previously shown to have the dual capacity to bind Grb-2 as well as α-catenin [9,10].
T11502 53828-53831 CD denotes One
T11503 53841-53848 VBZ denotes appears
T11504 53832-53834 IN denotes of
T11505 53835-53840 DT denotes these
T11506 53849-53851 TO denotes to
T11507 53852-53854 VB denotes be
T11508 53855-53860 NN denotes Ajuba
T11509 53860-53862 , denotes ,
T11510 53862-53867 WDT denotes which
T11511 53883-53888 VBN denotes shown
T11512 53868-53871 VBD denotes was
T11513 53872-53882 RB denotes previously
T11514 53889-53891 TO denotes to
T11515 53892-53896 VB denotes have
T11516 53897-53900 DT denotes the
T11517 53906-53914 NN denotes capacity
T11518 53901-53905 JJ denotes dual
T11519 53915-53917 TO denotes to
T11520 53918-53922 VB denotes bind
T11521 53923-53926 NN denotes Grb
T11522 53926-53927 HYPH denotes -
T11523 53927-53928 CD denotes 2
T11524 53929-53931 RB denotes as
T11525 53937-53939 IN denotes as
T11526 53932-53936 RB denotes well
T11527 53940-53941 NN denotes α
T11528 53942-53949 NN denotes catenin
T11529 53941-53942 HYPH denotes -
T11530 53950-53951 -LRB- denotes [
T11531 53953-53955 CD denotes 10
T11532 53951-53952 CD denotes 9
T11533 53952-53953 , denotes ,
T11534 53955-53956 -RRB- denotes ]
T11535 53956-53957 . denotes .
T11536 53957-54185 sentence denotes Our studies revealed that in skin keratinocytes that either harbor a conditional null mutation in α-catenin or that overexpress Snail, Ajuba develops an interaction with Grb-2 that is otherwise not observed in WT keratinocytes.
T11537 53958-53961 PRP$ denotes Our
T11538 53962-53969 NNS denotes studies
T11539 53970-53978 VBD denotes revealed
T11540 53979-53983 IN denotes that
T11541 54099-54107 VBZ denotes develops
T11542 53984-53986 IN denotes in
T11543 53987-53991 NN denotes skin
T11544 53992-54005 NNS denotes keratinocytes
T11545 54006-54010 WDT denotes that
T11546 54018-54024 VBP denotes harbor
T11547 54011-54017 CC denotes either
T11548 54025-54026 DT denotes a
T11549 54044-54052 NN denotes mutation
T11550 54027-54038 JJ denotes conditional
T11551 54039-54043 JJ denotes null
T11552 54053-54055 IN denotes in
T11553 54056-54057 NN denotes α
T11554 54058-54065 NN denotes catenin
T11555 54057-54058 HYPH denotes -
T11556 54066-54068 CC denotes or
T11557 54069-54073 WDT denotes that
T11558 54074-54085 VBP denotes overexpress
T11559 54086-54091 NN denotes Snail
T11560 54091-54093 , denotes ,
T11561 54093-54098 NN denotes Ajuba
T11562 54108-54110 DT denotes an
T11563 54111-54122 NN denotes interaction
T11564 54123-54127 IN denotes with
T11565 54128-54131 NN denotes Grb
T11566 54131-54132 HYPH denotes -
T11567 54132-54133 CD denotes 2
T11568 54134-54138 WDT denotes that
T11569 54156-54164 VBN denotes observed
T11570 54139-54141 VBZ denotes is
T11571 54142-54151 RB denotes otherwise
T11572 54152-54155 RB denotes not
T11573 54165-54167 IN denotes in
T11574 54168-54170 NN denotes WT
T11575 54171-54184 NNS denotes keratinocytes
T11576 54184-54185 . denotes .
T11577 54185-54489 sentence denotes The corresponding abilities of either Snail-transfected or Ajuba-transfected keratinocytes to exhibit elevated activation of the Ras-MAPK pathway suggest that the Grb-2 association of Ajuba under conditions of reduced levels of AJ proteins may be directly relevant to the parallel in hyperproliferation.
T11578 54186-54189 DT denotes The
T11579 54204-54213 NNS denotes abilities
T11580 54190-54203 VBG denotes corresponding
T11581 54332-54339 VBP denotes suggest
T11582 54214-54216 IN denotes of
T11583 54217-54223 CC denotes either
T11584 54263-54276 NNS denotes keratinocytes
T11585 54224-54229 NN denotes Snail
T11586 54230-54241 VBN denotes transfected
T11587 54229-54230 HYPH denotes -
T11588 54242-54244 CC denotes or
T11589 54245-54250 NN denotes Ajuba
T11590 54251-54262 VBN denotes transfected
T11591 54250-54251 HYPH denotes -
T11592 54277-54279 TO denotes to
T11593 54280-54287 VB denotes exhibit
T11594 54288-54296 JJ denotes elevated
T11595 54297-54307 NN denotes activation
T11596 54308-54310 IN denotes of
T11597 54311-54314 DT denotes the
T11598 54324-54331 NN denotes pathway
T11599 54315-54318 NN denotes Ras
T11600 54319-54323 NN denotes MAPK
T11601 54318-54319 HYPH denotes -
T11602 54340-54344 IN denotes that
T11603 54430-54432 VB denotes be
T11604 54345-54348 DT denotes the
T11605 54355-54366 NN denotes association
T11606 54349-54352 NN denotes Grb
T11607 54352-54353 HYPH denotes -
T11608 54353-54354 CD denotes 2
T11609 54367-54369 IN denotes of
T11610 54370-54375 NN denotes Ajuba
T11611 54376-54381 IN denotes under
T11612 54382-54392 NNS denotes conditions
T11613 54393-54395 IN denotes of
T11614 54396-54403 JJ denotes reduced
T11615 54404-54410 NNS denotes levels
T11616 54411-54413 IN denotes of
T11617 54414-54416 NN denotes AJ
T11618 54417-54425 NN denotes proteins
T11619 54426-54429 MD denotes may
T11620 54433-54441 RB denotes directly
T11621 54442-54450 JJ denotes relevant
T11622 54451-54453 IN denotes to
T11623 54454-54457 DT denotes the
T11624 54458-54466 NN denotes parallel
T11625 54467-54469 IN denotes in
T11626 54470-54488 NN denotes hyperproliferation
T11627 54488-54489 . denotes .
T11628 54489-54676 sentence denotes In stable epithelial (i.e., Madin-Darby canine kidney, or MDCK) cell lines, Snail has been shown to block cell cycle progression and promote motility and shape changes for invasion [47].
T11629 54490-54492 IN denotes In
T11630 54581-54586 VBN denotes shown
T11631 54493-54499 JJ denotes stable
T11632 54559-54564 NNS denotes lines
T11633 54500-54510 JJ denotes epithelial
T11634 54511-54512 -LRB- denotes (
T11635 54537-54543 NN denotes kidney
T11636 54512-54516 FW denotes i.e.
T11637 54516-54518 , denotes ,
T11638 54518-54523 NNP denotes Madin
T11639 54524-54529 NNP denotes Darby
T11640 54523-54524 HYPH denotes -
T11641 54530-54536 JJ denotes canine
T11642 54543-54545 , denotes ,
T11643 54545-54547 CC denotes or
T11644 54548-54552 NN denotes MDCK
T11645 54552-54553 -RRB- denotes )
T11646 54554-54558 NN denotes cell
T11647 54564-54566 , denotes ,
T11648 54566-54571 NN denotes Snail
T11649 54572-54575 VBZ denotes has
T11650 54576-54580 VBN denotes been
T11651 54587-54589 TO denotes to
T11652 54590-54595 VB denotes block
T11653 54596-54600 NN denotes cell
T11654 54601-54606 NN denotes cycle
T11655 54607-54618 NN denotes progression
T11656 54619-54622 CC denotes and
T11657 54623-54630 VB denotes promote
T11658 54631-54639 NN denotes motility
T11659 54650-54657 NNS denotes changes
T11660 54640-54643 CC denotes and
T11661 54644-54649 NN denotes shape
T11662 54658-54661 IN denotes for
T11663 54662-54670 NN denotes invasion
T11664 54671-54672 -LRB- denotes [
T11665 54672-54674 CD denotes 47
T11666 54674-54675 -RRB- denotes ]
T11667 54675-54676 . denotes .
T11668 54676-54846 sentence denotes While our in vivo studies are consistent with a role for Snail in motility and epithelial remodeling, they differ with respect to Snail's apparent proliferative effects.
T11669 54677-54682 IN denotes While
T11670 54703-54706 VBP denotes are
T11671 54683-54686 PRP$ denotes our
T11672 54695-54702 NNS denotes studies
T11673 54687-54689 FW denotes in
T11674 54690-54694 FW denotes vivo
T11675 54784-54790 VBP denotes differ
T11676 54707-54717 JJ denotes consistent
T11677 54718-54722 IN denotes with
T11678 54723-54724 DT denotes a
T11679 54725-54729 NN denotes role
T11680 54730-54733 IN denotes for
T11681 54734-54739 NN denotes Snail
T11682 54740-54742 IN denotes in
T11683 54743-54751 NN denotes motility
T11684 54767-54777 NN denotes remodeling
T11685 54752-54755 CC denotes and
T11686 54756-54766 JJ denotes epithelial
T11687 54777-54779 , denotes ,
T11688 54779-54783 PRP denotes they
T11689 54791-54795 IN denotes with
T11690 54796-54803 NN denotes respect
T11691 54804-54806 IN denotes to
T11692 54807-54812 NN denotes Snail
T11693 54838-54845 NNS denotes effects
T11694 54812-54814 POS denotes 's
T11695 54815-54823 JJ denotes apparent
T11696 54824-54837 JJ denotes proliferative
T11697 54845-54846 . denotes .
T11698 54846-54956 sentence denotes A priori, this could be simply due to variations in the response of different cell types to Snail expression.
T11699 54847-54848 FW denotes A
T11700 54849-54855 FW denotes priori
T11701 54868-54870 VB denotes be
T11702 54855-54857 , denotes ,
T11703 54857-54861 DT denotes this
T11704 54862-54867 MD denotes could
T11705 54871-54877 RB denotes simply
T11706 54878-54881 IN denotes due
T11707 54882-54884 IN denotes to
T11708 54885-54895 NNS denotes variations
T11709 54896-54898 IN denotes in
T11710 54899-54902 DT denotes the
T11711 54903-54911 NN denotes response
T11712 54912-54914 IN denotes of
T11713 54915-54924 JJ denotes different
T11714 54930-54935 NNS denotes types
T11715 54925-54929 NN denotes cell
T11716 54936-54938 IN denotes to
T11717 54939-54944 NN denotes Snail
T11718 54945-54955 NN denotes expression
T11719 54955-54956 . denotes .
T11720 54956-55115 sentence denotes Alternatively, these differences may be relevant to the benefit of using mouse models to reveal functions not always recapitulated in stable cell line models.
T11721 54957-54970 RB denotes Alternatively
T11722 54994-54996 VB denotes be
T11723 54970-54972 , denotes ,
T11724 54972-54977 DT denotes these
T11725 54978-54989 NNS denotes differences
T11726 54990-54993 MD denotes may
T11727 54997-55005 JJ denotes relevant
T11728 55006-55008 IN denotes to
T11729 55009-55012 DT denotes the
T11730 55013-55020 NN denotes benefit
T11731 55021-55023 IN denotes of
T11732 55024-55029 VBG denotes using
T11733 55030-55035 NN denotes mouse
T11734 55036-55042 NNS denotes models
T11735 55043-55045 TO denotes to
T11736 55046-55052 VB denotes reveal
T11737 55053-55062 NNS denotes functions
T11738 55063-55066 RB denotes not
T11739 55074-55087 VBN denotes recapitulated
T11740 55067-55073 RB denotes always
T11741 55088-55090 IN denotes in
T11742 55091-55097 JJ denotes stable
T11743 55108-55114 NNS denotes models
T11744 55098-55102 NN denotes cell
T11745 55103-55107 NN denotes line
T11746 55114-55115 . denotes .
T11747 55115-55198 sentence denotes Future studies should highlight the underlying reasons for these opposing results.
T11748 55116-55122 JJ denotes Future
T11749 55123-55130 NNS denotes studies
T11750 55138-55147 VB denotes highlight
T11751 55131-55137 MD denotes should
T11752 55148-55151 DT denotes the
T11753 55163-55170 NNS denotes reasons
T11754 55152-55162 JJ denotes underlying
T11755 55171-55174 IN denotes for
T11756 55175-55180 DT denotes these
T11757 55190-55197 NNS denotes results
T11758 55181-55189 VBG denotes opposing
T11759 55197-55198 . denotes .
T11760 55198-55383 sentence denotes Irrespective of these differences, our in vivo studies do not stand alone, as there are many situations in which a down-regulation in AJ proteins correlate with enhanced proliferation.
T11761 55199-55211 JJ denotes Irrespective
T11762 55261-55266 VB denotes stand
T11763 55212-55214 IN denotes of
T11764 55215-55220 DT denotes these
T11765 55221-55232 NNS denotes differences
T11766 55232-55234 , denotes ,
T11767 55234-55237 PRP$ denotes our
T11768 55246-55253 NNS denotes studies
T11769 55238-55240 FW denotes in
T11770 55241-55245 FW denotes vivo
T11771 55254-55256 VBP denotes do
T11772 55257-55260 RB denotes not
T11773 55267-55272 RB denotes alone
T11774 55272-55274 , denotes ,
T11775 55274-55276 IN denotes as
T11776 55283-55286 VBP denotes are
T11777 55277-55282 EX denotes there
T11778 55287-55291 JJ denotes many
T11779 55292-55302 NNS denotes situations
T11780 55303-55305 IN denotes in
T11781 55345-55354 VBP denotes correlate
T11782 55306-55311 WDT denotes which
T11783 55312-55313 DT denotes a
T11784 55319-55329 NN denotes regulation
T11785 55314-55318 JJ denotes down
T11786 55318-55319 HYPH denotes -
T11787 55330-55332 IN denotes in
T11788 55333-55335 NN denotes AJ
T11789 55336-55344 NN denotes proteins
T11790 55355-55359 IN denotes with
T11791 55360-55368 VBN denotes enhanced
T11792 55369-55382 NN denotes proliferation
T11793 55382-55383 . denotes .
T11794 55383-55533 sentence denotes In fact, a myriad of diverse mechanisms have been implicated in activating epithelial proliferation upon down-regulation of AJ proteins [7,23,24,48].
T11795 55384-55386 IN denotes In
T11796 55434-55444 VBN denotes implicated
T11797 55387-55391 NN denotes fact
T11798 55391-55393 , denotes ,
T11799 55393-55394 DT denotes a
T11800 55395-55401 NN denotes myriad
T11801 55402-55404 IN denotes of
T11802 55405-55412 JJ denotes diverse
T11803 55413-55423 NNS denotes mechanisms
T11804 55424-55428 VBP denotes have
T11805 55429-55433 VBN denotes been
T11806 55445-55447 IN denotes in
T11807 55448-55458 VBG denotes activating
T11808 55459-55469 JJ denotes epithelial
T11809 55470-55483 NN denotes proliferation
T11810 55484-55488 IN denotes upon
T11811 55489-55493 JJ denotes down
T11812 55494-55504 NN denotes regulation
T11813 55493-55494 HYPH denotes -
T11814 55505-55507 IN denotes of
T11815 55508-55510 NN denotes AJ
T11816 55511-55519 NN denotes proteins
T11817 55520-55521 -LRB- denotes [
T11818 55529-55531 CD denotes 48
T11819 55521-55522 CD denotes 7
T11820 55522-55523 , denotes ,
T11821 55523-55525 CD denotes 23
T11822 55525-55526 , denotes ,
T11823 55526-55528 CD denotes 24
T11824 55528-55529 , denotes ,
T11825 55531-55532 -RRB- denotes ]
T11826 55532-55533 . denotes .
T11827 55533-55628 sentence denotes Sifting through these converging pathways is likely to be a difficult and painstaking process.
T11828 55534-55541 VBG denotes Sifting
T11829 55576-55578 VBZ denotes is
T11830 55542-55549 IN denotes through
T11831 55550-55555 DT denotes these
T11832 55567-55575 NNS denotes pathways
T11833 55556-55566 VBG denotes converging
T11834 55579-55585 JJ denotes likely
T11835 55586-55588 TO denotes to
T11836 55589-55591 VB denotes be
T11837 55592-55593 DT denotes a
T11838 55620-55627 NN denotes process
T11839 55594-55603 JJ denotes difficult
T11840 55604-55607 CC denotes and
T11841 55608-55619 JJ denotes painstaking
T11842 55627-55628 . denotes .
T11843 55628-55913 sentence denotes This said, by identifying the status of different players involved in specific cell types and at specific stages in development, our mechanistic understanding of how intercellular remodeling is linked to proliferation in epithelial morphogenesis should begin to surface in the future.
T11844 55629-55633 DT denotes This
T11845 55634-55638 VBD denotes said
T11846 55882-55887 VB denotes begin
T11847 55638-55640 , denotes ,
T11848 55640-55642 IN denotes by
T11849 55643-55654 VBG denotes identifying
T11850 55655-55658 DT denotes the
T11851 55659-55665 NN denotes status
T11852 55666-55668 IN denotes of
T11853 55669-55678 JJ denotes different
T11854 55679-55686 NNS denotes players
T11855 55687-55695 VBN denotes involved
T11856 55696-55698 IN denotes in
T11857 55699-55707 JJ denotes specific
T11858 55713-55718 NNS denotes types
T11859 55708-55712 NN denotes cell
T11860 55719-55722 CC denotes and
T11861 55723-55725 IN denotes at
T11862 55726-55734 JJ denotes specific
T11863 55735-55741 NNS denotes stages
T11864 55742-55744 IN denotes in
T11865 55745-55756 NN denotes development
T11866 55756-55758 , denotes ,
T11867 55758-55761 PRP$ denotes our
T11868 55774-55787 NN denotes understanding
T11869 55762-55773 JJ denotes mechanistic
T11870 55788-55790 IN denotes of
T11871 55791-55794 WRB denotes how
T11872 55823-55829 VBN denotes linked
T11873 55795-55808 JJ denotes intercellular
T11874 55809-55819 NN denotes remodeling
T11875 55820-55822 VBZ denotes is
T11876 55830-55832 IN denotes to
T11877 55833-55846 NN denotes proliferation
T11878 55847-55849 IN denotes in
T11879 55850-55860 JJ denotes epithelial
T11880 55861-55874 NN denotes morphogenesis
T11881 55875-55881 MD denotes should
T11882 55888-55890 TO denotes to
T11883 55891-55898 VB denotes surface
T11884 55899-55901 IN denotes in
T11885 55902-55905 DT denotes the
T11886 55906-55912 NN denotes future
T11887 55912-55913 . denotes .
T11888 55913-56079 sentence denotes Elucidating the molecular mechanisms through which these networks converge is also a prerequisite for understanding how these processes go awry during tumorigenesis.
T11889 55914-55925 VBG denotes Elucidating
T11890 55989-55991 VBZ denotes is
T11891 55926-55929 DT denotes the
T11892 55940-55950 NNS denotes mechanisms
T11893 55930-55939 JJ denotes molecular
T11894 55951-55958 IN denotes through
T11895 55980-55988 VBP denotes converge
T11896 55959-55964 WDT denotes which
T11897 55965-55970 DT denotes these
T11898 55971-55979 NNS denotes networks
T11899 55992-55996 RB denotes also
T11900 55997-55998 DT denotes a
T11901 55999-56011 NN denotes prerequisite
T11902 56012-56015 IN denotes for
T11903 56016-56029 VBG denotes understanding
T11904 56030-56033 WRB denotes how
T11905 56050-56052 VBP denotes go
T11906 56034-56039 DT denotes these
T11907 56040-56049 NNS denotes processes
T11908 56053-56057 RB denotes awry
T11909 56058-56064 IN denotes during
T11910 56065-56078 NN denotes tumorigenesis
T11911 56078-56079 . denotes .
T12021 56104-56112 NNS denotes Reagents
T12022 56112-57203 sentence denotes Primary antibodies used were against: E-cadherin (M. Takeichi, Kyoto University, Japan); α-catenin, β-catenin, pMAPK, tubulin (Sigma, St. Louis, Missouri, United States), Ajuba (G. Longmore, Washington University, St. Louis, Missouri, United States); β4 integrin/CD104 (BD Pharmingen, San Diego, California, United States), laminin 5 (R. Burgeson, Harvard University, Cambridge, Massachusetts, United States), K5, K1, loricrin (Fuchs Lab), involucrin, fillagrin (Covance, Berkeley, California, United States), MAPK, pSMAD2 (Cell Signaling, Beverly, Massachusetts, United States); Grb-2 (Santa Cruz Biotech, Santa Cruz, California, United States); P-cadherin (Zymed Laboratories, South San Francisco, California, United States); HA (Roche Biochemicals), vimentin (Chemicon, Temecula, California, United States), Ki67 (Novo Castra, Newcastle Upon Tyne, United Kingdom), keratin 6 (P. Coulombe, John Hopkins University, Baltimore, Maryland, United States), cyclin D (Oncogene, San Diego, California, United States), and TGF-β2 (L. Gold, New York University, New York, New York, United States).
T12023 56113-56120 JJ denotes Primary
T12024 56121-56131 NNS denotes antibodies
T12025 56137-56141 VBD denotes were
T12026 56132-56136 VBN denotes used
T12027 56142-56149 IN denotes against
T12028 56149-56151 : denotes :
T12029 56151-56152 NN denotes E
T12030 56153-56161 NN denotes cadherin
T12031 56152-56153 HYPH denotes -
T12032 56162-56163 -LRB- denotes (
T12033 56166-56174 NNP denotes Takeichi
T12034 56163-56165 NNP denotes M.
T12035 56174-56176 , denotes ,
T12036 56176-56181 NNP denotes Kyoto
T12037 56182-56192 NNP denotes University
T12038 56192-56194 , denotes ,
T12039 56194-56199 NNP denotes Japan
T12040 56199-56200 -RRB- denotes )
T12041 56200-56201 : denotes ;
T12042 56202-56203 NN denotes α
T12043 56204-56211 NN denotes catenin
T12044 56203-56204 HYPH denotes -
T12045 56211-56213 , denotes ,
T12046 56213-56214 NN denotes β
T12047 56215-56222 NN denotes catenin
T12048 56214-56215 HYPH denotes -
T12049 56222-56224 , denotes ,
T12050 56224-56229 NN denotes pMAPK
T12051 56229-56231 , denotes ,
T12052 56231-56238 NN denotes tubulin
T12053 56239-56240 -LRB- denotes (
T12054 56240-56245 NNP denotes Sigma
T12055 56245-56247 , denotes ,
T12056 56247-56250 NNP denotes St.
T12057 56251-56256 NNP denotes Louis
T12058 56256-56258 , denotes ,
T12059 56258-56266 NNP denotes Missouri
T12060 56266-56268 , denotes ,
T12061 56268-56274 NNP denotes United
T12062 56275-56281 NNP denotes States
T12063 56281-56282 -RRB- denotes )
T12064 56282-56284 , denotes ,
T12065 56284-56289 NN denotes Ajuba
T12066 56290-56291 -LRB- denotes (
T12067 56294-56302 NNP denotes Longmore
T12068 56291-56293 NNP denotes G.
T12069 56302-56304 , denotes ,
T12070 56304-56314 NNP denotes Washington
T12071 56315-56325 NNP denotes University
T12072 56325-56327 , denotes ,
T12073 56327-56330 NNP denotes St.
T12074 56331-56336 NNP denotes Louis
T12075 56336-56338 , denotes ,
T12076 56338-56346 NNP denotes Missouri
T12077 56346-56348 , denotes ,
T12078 56348-56354 NNP denotes United
T12079 56355-56361 NNP denotes States
T12080 56361-56362 -RRB- denotes )
T12081 56362-56363 : denotes ;
T12082 56364-56366 NN denotes β4
T12083 56367-56375 NN denotes integrin
T12084 56375-56376 HYPH denotes /
T12085 56376-56381 NN denotes CD104
T12086 56382-56383 -LRB- denotes (
T12087 56386-56396 NNP denotes Pharmingen
T12088 56383-56385 NNP denotes BD
T12089 56396-56398 , denotes ,
T12090 56398-56401 NNP denotes San
T12091 56402-56407 NNP denotes Diego
T12092 56407-56409 , denotes ,
T12093 56409-56419 NNP denotes California
T12094 56419-56421 , denotes ,
T12095 56421-56427 NNP denotes United
T12096 56428-56434 NNP denotes States
T12097 56434-56435 -RRB- denotes )
T12098 56435-56437 , denotes ,
T12099 56437-56444 NN denotes laminin
T12100 56445-56446 CD denotes 5
T12101 56447-56448 -LRB- denotes (
T12102 56451-56459 NNP denotes Burgeson
T12103 56448-56450 NNP denotes R.
T12104 56459-56461 , denotes ,
T12105 56461-56468 NNP denotes Harvard
T12106 56469-56479 NNP denotes University
T12107 56479-56481 , denotes ,
T12108 56481-56490 NNP denotes Cambridge
T12109 56490-56492 , denotes ,
T12110 56492-56505 NNP denotes Massachusetts
T12111 56505-56507 , denotes ,
T12112 56507-56513 NNP denotes United
T12113 56514-56520 NNP denotes States
T12114 56520-56521 -RRB- denotes )
T12115 56521-56523 , denotes ,
T12116 56523-56525 NN denotes K5
T12117 56525-56527 , denotes ,
T12118 56527-56529 NN denotes K1
T12119 56529-56531 , denotes ,
T12120 56531-56539 NN denotes loricrin
T12121 56540-56541 -LRB- denotes (
T12122 56547-56550 NNP denotes Lab
T12123 56541-56546 NNP denotes Fuchs
T12124 56550-56551 -RRB- denotes )
T12125 56551-56553 , denotes ,
T12126 56553-56563 NN denotes involucrin
T12127 56563-56565 , denotes ,
T12128 56565-56574 NN denotes fillagrin
T12129 56575-56576 -LRB- denotes (
T12130 56576-56583 NNP denotes Covance
T12131 56583-56585 , denotes ,
T12132 56585-56593 NNP denotes Berkeley
T12133 56593-56595 , denotes ,
T12134 56595-56605 NNP denotes California
T12135 56605-56607 , denotes ,
T12136 56607-56613 NNP denotes United
T12137 56614-56620 NNP denotes States
T12138 56620-56621 -RRB- denotes )
T12139 56621-56623 , denotes ,
T12140 56623-56627 NN denotes MAPK
T12141 56627-56629 , denotes ,
T12142 56629-56635 NN denotes pSMAD2
T12143 56636-56637 -LRB- denotes (
T12144 56642-56651 NNP denotes Signaling
T12145 56637-56641 NNP denotes Cell
T12146 56651-56653 , denotes ,
T12147 56653-56660 NNP denotes Beverly
T12148 56660-56662 , denotes ,
T12149 56662-56675 NNP denotes Massachusetts
T12150 56675-56677 , denotes ,
T12151 56677-56683 NNP denotes United
T12152 56684-56690 NNP denotes States
T12153 56690-56691 -RRB- denotes )
T12154 56691-56692 : denotes ;
T12155 56693-56696 NN denotes Grb
T12156 56696-56697 HYPH denotes -
T12157 56697-56698 CD denotes 2
T12158 56699-56700 -LRB- denotes (
T12159 56711-56718 NNP denotes Biotech
T12160 56700-56705 NNP denotes Santa
T12161 56706-56710 NNP denotes Cruz
T12162 56718-56720 , denotes ,
T12163 56720-56725 NNP denotes Santa
T12164 56726-56730 NNP denotes Cruz
T12165 56730-56732 , denotes ,
T12166 56732-56742 NNP denotes California
T12167 56742-56744 , denotes ,
T12168 56744-56750 NNP denotes United
T12169 56751-56757 NNP denotes States
T12170 56757-56758 -RRB- denotes )
T12171 56758-56759 : denotes ;
T12172 56760-56761 NN denotes P
T12173 56762-56770 NN denotes cadherin
T12174 56761-56762 HYPH denotes -
T12175 56771-56772 -LRB- denotes (
T12176 56778-56790 NNP denotes Laboratories
T12177 56772-56777 NNP denotes Zymed
T12178 56790-56792 , denotes ,
T12179 56792-56797 JJ denotes South
T12180 56802-56811 NNP denotes Francisco
T12181 56798-56801 NNP denotes San
T12182 56811-56813 , denotes ,
T12183 56813-56823 NNP denotes California
T12184 56823-56825 , denotes ,
T12185 56825-56831 NNP denotes United
T12186 56832-56838 NNP denotes States
T12187 56838-56839 -RRB- denotes )
T12188 56839-56840 : denotes ;
T12189 56841-56843 NN denotes HA
T12190 56844-56845 -LRB- denotes (
T12191 56851-56863 NNP denotes Biochemicals
T12192 56845-56850 NNP denotes Roche
T12193 56863-56864 -RRB- denotes )
T12194 56864-56866 , denotes ,
T12195 56866-56874 NN denotes vimentin
T12196 56875-56876 -LRB- denotes (
T12197 56876-56884 NNP denotes Chemicon
T12198 56884-56886 , denotes ,
T12199 56886-56894 NNP denotes Temecula
T12200 56894-56896 , denotes ,
T12201 56896-56906 NNP denotes California
T12202 56906-56908 , denotes ,
T12203 56908-56914 NNP denotes United
T12204 56915-56921 NNP denotes States
T12205 56921-56922 -RRB- denotes )
T12206 56922-56924 , denotes ,
T12207 56924-56928 NN denotes Ki67
T12208 56929-56930 -LRB- denotes (
T12209 56935-56941 NNP denotes Castra
T12210 56930-56934 NNP denotes Novo
T12211 56941-56943 , denotes ,
T12212 56943-56952 NNP denotes Newcastle
T12213 56953-56957 IN denotes Upon
T12214 56958-56962 NNP denotes Tyne
T12215 56962-56964 , denotes ,
T12216 56964-56970 NNP denotes United
T12217 56971-56978 NNP denotes Kingdom
T12218 56978-56979 -RRB- denotes )
T12219 56979-56981 , denotes ,
T12220 56981-56988 NN denotes keratin
T12221 56989-56990 CD denotes 6
T12222 56991-56992 -LRB- denotes (
T12223 56995-57003 NNP denotes Coulombe
T12224 56992-56994 NNP denotes P.
T12225 57003-57005 , denotes ,
T12226 57005-57009 NNP denotes John
T12227 57010-57017 NNP denotes Hopkins
T12228 57018-57028 NNP denotes University
T12229 57028-57030 , denotes ,
T12230 57030-57039 NNP denotes Baltimore
T12231 57039-57041 , denotes ,
T12232 57041-57049 NNP denotes Maryland
T12233 57049-57051 , denotes ,
T12234 57051-57057 NNP denotes United
T12235 57058-57064 NNP denotes States
T12236 57064-57065 -RRB- denotes )
T12237 57065-57067 , denotes ,
T12238 57067-57073 NN denotes cyclin
T12239 57074-57075 NN denotes D
T12240 57076-57077 -LRB- denotes (
T12241 57077-57085 NNP denotes Oncogene
T12242 57085-57087 , denotes ,
T12243 57087-57090 NNP denotes San
T12244 57091-57096 NNP denotes Diego
T12245 57096-57098 , denotes ,
T12246 57098-57108 NNP denotes California
T12247 57108-57110 , denotes ,
T12248 57110-57116 NNP denotes United
T12249 57117-57123 NNP denotes States
T12250 57123-57124 -RRB- denotes )
T12251 57124-57126 , denotes ,
T12252 57126-57129 CC denotes and
T12253 57130-57133 NN denotes TGF
T12254 57134-57136 NN denotes β2
T12255 57133-57134 HYPH denotes -
T12256 57137-57138 -LRB- denotes (
T12257 57141-57145 NNP denotes Gold
T12258 57138-57140 NNP denotes L.
T12259 57145-57147 , denotes ,
T12260 57147-57150 NNP denotes New
T12261 57151-57155 NNP denotes York
T12262 57156-57166 NNP denotes University
T12263 57166-57168 , denotes ,
T12264 57168-57171 NNP denotes New
T12265 57172-57176 NNP denotes York
T12266 57176-57178 , denotes ,
T12267 57178-57181 NNP denotes New
T12268 57182-57186 NNP denotes York
T12269 57186-57188 , denotes ,
T12270 57188-57194 NNP denotes United
T12271 57195-57201 NNP denotes States
T12272 57201-57202 -RRB- denotes )
T12273 57202-57203 . denotes .
T12274 57203-57337 sentence denotes FITC-, Texas Red-, or HRP-conjugated secondary antibodies were from Jackson ImmunoResearch (West Grove, Pennsylvania, United States).
T12275 57204-57208 NN denotes FITC
T12276 57230-57240 VBN denotes conjugated
T12277 57208-57209 HYPH denotes -
T12278 57209-57211 , denotes ,
T12279 57211-57216 NNP denotes Texas
T12280 57217-57220 NNP denotes Red
T12281 57220-57221 HYPH denotes -
T12282 57221-57223 , denotes ,
T12283 57223-57225 CC denotes or
T12284 57226-57229 NN denotes HRP
T12285 57229-57230 HYPH denotes -
T12286 57251-57261 NNS denotes antibodies
T12287 57241-57250 JJ denotes secondary
T12288 57262-57266 VBD denotes were
T12289 57267-57271 IN denotes from
T12290 57272-57279 NNP denotes Jackson
T12291 57280-57294 NNP denotes ImmunoResearch
T12292 57295-57296 -LRB- denotes (
T12293 57301-57306 NNP denotes Grove
T12294 57296-57300 NNP denotes West
T12295 57306-57308 , denotes ,
T12296 57308-57320 NNP denotes Pennsylvania
T12297 57320-57322 , denotes ,
T12298 57322-57328 NNP denotes United
T12299 57329-57335 NNP denotes States
T12300 57335-57336 -RRB- denotes )
T12301 57336-57337 . denotes .
T12302 57337-57434 sentence denotes Biotinylated secondary antibodies were from Vector Labs (Burlingame, California, United States).
T12303 57338-57350 VBN denotes Biotinylated
T12304 57361-57371 NNS denotes antibodies
T12305 57351-57360 JJ denotes secondary
T12306 57372-57376 VBD denotes were
T12307 57377-57381 IN denotes from
T12308 57382-57388 NNP denotes Vector
T12309 57389-57393 NNP denotes Labs
T12310 57394-57395 -LRB- denotes (
T12311 57395-57405 NNP denotes Burlingame
T12312 57405-57407 , denotes ,
T12313 57407-57417 NNP denotes California
T12314 57417-57419 , denotes ,
T12315 57419-57425 NNP denotes United
T12316 57426-57432 NNP denotes States
T12317 57432-57433 -RRB- denotes )
T12318 57433-57434 . denotes .
T12319 57434-57497 sentence denotes Dilutions were according to the manufacturer's recommendation.
T12320 57435-57444 NNS denotes Dilutions
T12321 57445-57449 VBD denotes were
T12322 57450-57459 VBG denotes according
T12323 57460-57462 IN denotes to
T12324 57463-57466 DT denotes the
T12325 57467-57479 NN denotes manufacturer
T12326 57482-57496 NN denotes recommendation
T12327 57479-57481 POS denotes 's
T12328 57496-57497 . denotes .
T12329 57497-57672 sentence denotes The Snail antibody was generated in Guinea pigs by inoculating them with the N-terminal sequence of murine Snail fused to GST (Covance, Princeton, New Jersey, United States).
T12330 57498-57501 DT denotes The
T12331 57508-57516 NN denotes antibody
T12332 57502-57507 NN denotes Snail
T12333 57521-57530 VBN denotes generated
T12334 57517-57520 VBD denotes was
T12335 57531-57533 IN denotes in
T12336 57534-57540 NN denotes Guinea
T12337 57541-57545 NNS denotes pigs
T12338 57546-57548 IN denotes by
T12339 57549-57560 VBG denotes inoculating
T12340 57561-57565 PRP denotes them
T12341 57566-57570 IN denotes with
T12342 57571-57574 DT denotes the
T12343 57586-57594 NN denotes sequence
T12344 57575-57576 NN denotes N
T12345 57577-57585 JJ denotes terminal
T12346 57576-57577 HYPH denotes -
T12347 57595-57597 IN denotes of
T12348 57598-57604 JJ denotes murine
T12349 57605-57610 NN denotes Snail
T12350 57611-57616 VBN denotes fused
T12351 57617-57619 IN denotes to
T12352 57620-57623 NN denotes GST
T12353 57624-57625 -LRB- denotes (
T12354 57625-57632 NNP denotes Covance
T12355 57632-57634 , denotes ,
T12356 57634-57643 NNP denotes Princeton
T12357 57643-57645 , denotes ,
T12358 57645-57648 NNP denotes New
T12359 57649-57655 NNP denotes Jersey
T12360 57655-57657 , denotes ,
T12361 57657-57663 NNP denotes United
T12362 57664-57670 NNP denotes States
T12363 57670-57671 -RRB- denotes )
T12364 57671-57672 . denotes .
T12365 57672-57761 sentence denotes Recombinant human TGF-β2 was purchased from R&D (Minneapolis, Minnesota, United States).
T12366 57673-57684 JJ denotes Recombinant
T12367 57695-57697 NN denotes β2
T12368 57685-57690 JJ denotes human
T12369 57691-57694 NN denotes TGF
T12370 57694-57695 HYPH denotes -
T12371 57702-57711 VBN denotes purchased
T12372 57698-57701 VBD denotes was
T12373 57712-57716 IN denotes from
T12374 57717-57718 NNP denotes R
T12375 57718-57719 CC denotes &
T12376 57719-57720 NNP denotes D
T12377 57721-57722 -LRB- denotes (
T12378 57722-57733 NNP denotes Minneapolis
T12379 57733-57735 , denotes ,
T12380 57735-57744 NNP denotes Minnesota
T12381 57744-57746 , denotes ,
T12382 57746-57752 NNP denotes United
T12383 57753-57759 NNP denotes States
T12384 57759-57760 -RRB- denotes )
T12385 57760-57761 . denotes .
T12386 57761-57856 sentence denotes Heat inactivated TGF-β2 was generated by heating the recombinant protein at 100 °C for 10 min.
T12387 57762-57766 NN denotes Heat
T12388 57767-57778 VBN denotes inactivated
T12389 57783-57785 NN denotes β2
T12390 57779-57782 NN denotes TGF
T12391 57782-57783 HYPH denotes -
T12392 57790-57799 VBN denotes generated
T12393 57786-57789 VBD denotes was
T12394 57800-57802 IN denotes by
T12395 57803-57810 VBG denotes heating
T12396 57811-57814 DT denotes the
T12397 57827-57834 NN denotes protein
T12398 57815-57826 JJ denotes recombinant
T12399 57835-57837 IN denotes at
T12400 57838-57841 CD denotes 100
T12401 57842-57844 NN denotes °C
T12402 57845-57848 IN denotes for
T12403 57849-57851 CD denotes 10
T12404 57852-57855 NN denotes min
T12405 57855-57856 . denotes .
T14008 63750-63752 NN denotes RT
T9016 45287-45292 NN denotes Snail
T14035 63881-63884 VBD denotes was
T14036 63895-63900 VBG denotes using
T14037 63901-63906 NN denotes oligo
T14038 63907-63909 NN denotes dT
T14039 63906-63907 HYPH denotes -
T14040 63910-63917 NNS denotes primers
T14041 63918-63921 CC denotes and
T14042 63922-63925 DT denotes the
T14043 63941-63944 NN denotes kit
T14044 63926-63937 NNP denotes Superscript
T14045 63938-63940 CD denotes II
T14046 63945-63946 -LRB- denotes (
T14047 63946-63956 NNP denotes Invitrogen
T14048 63956-63957 -RRB- denotes )
T14049 63957-63958 . denotes .
T14050 63958-64171 sentence denotes The primers used for PCR were Snail forward: 5′- CAGCTGGCCAGGCTCTCGGT-3′; Snail reverse: 5′- GCGAGGGCCTCCGGAGCA-3′; GAPDH forward 5′- CGTAGACAAAATGGTGAAGGTCGG-3′; and GAPDH reverse: 5′- AAGCAGTTGGTGGTGCAGGATG-3′.
T14051 63959-63962 DT denotes The
T14052 63963-63970 NNS denotes primers
T14053 63984-63988 VBD denotes were
T14054 63971-63975 VBN denotes used
T14055 63976-63979 IN denotes for
T14056 63980-63983 NN denotes PCR
T14057 63989-63994 NN denotes Snail
T14058 63995-64002 NN denotes forward
T14059 64002-64004 : denotes :
T14060 64004-64005 CD denotes 5
T14061 64008-64028 NN denotes CAGCTGGCCAGGCTCTCGGT
T14062 64005-64006 SYM denotes ′
T14063 64006-64007 HYPH denotes -
T14064 64028-64029 HYPH denotes -
T9017 45298-45308 NN denotes expression
T9018 45293-45297 NN denotes gene
T146 0-1 DT denotes A
T9962 47697-47704 VB denotes promote
T8982 45112-45113 . denotes .
T8983 45113-45254 sentence denotes These data suggest that during hair follicle morphogenesis, TGF-β2 functions subsequently to Wnt/noggin-mediated determination of hair fate.
T8984 45114-45119 DT denotes These
T8985 45120-45124 NNS denotes data
T8986 45125-45132 VBP denotes suggest
T8987 45133-45137 IN denotes that
T8988 45181-45190 VBZ denotes functions
T8989 45138-45144 IN denotes during
T8990 45145-45149 NN denotes hair
T8991 45150-45158 NN denotes follicle
T8992 45159-45172 NN denotes morphogenesis
T8993 45172-45174 , denotes ,
T8994 45174-45177 NN denotes TGF
T8995 45178-45180 NN denotes β2
T8996 45177-45178 HYPH denotes -
T8997 45191-45203 RB denotes subsequently
T8998 45204-45206 IN denotes to
T8999 45207-45210 NN denotes Wnt
T9000 45211-45217 NN denotes noggin
T9001 45210-45211 HYPH denotes /
T9002 45218-45226 VBN denotes mediated
T9003 45217-45218 HYPH denotes -
T9004 45227-45240 NN denotes determination
T9005 45241-45243 IN denotes of
T9006 45244-45248 NN denotes hair
T9007 45249-45253 NN denotes fate
T9008 45253-45254 . denotes .
T9009 45254-45472 sentence denotes Moreover, through activation of Snail gene expression, TGF-β2 appears to work in tandem with these other morphogens to down-regulate E-cadherin levels, which contributes to the activation of proliferative circuitries.
T9010 45255-45263 RB denotes Moreover
T9011 45317-45324 VBZ denotes appears
T9012 45263-45265 , denotes ,
T9013 45265-45272 IN denotes through
T9014 45273-45283 NN denotes activation
T9015 45284-45286 IN denotes of
R5 T152 T150 dobj β2,Involving
R6 T153 T152 punct -,β2
R15 T165 T162 pobj theme,In
R16 T166 T165 amod common,theme
R24 T174 T172 pobj sheet,within
R25 T175 T174 amod multipotent,sheet
R26 T176 T174 amod epithelial,sheet
R35 T185 T183 pobj structure,into
R36 T186 T185 compound bud,structure
R59 T211 T209 pobj cues,of
R60 T212 T211 amod extracellular,cues
R77 T231 T229 ccomp is,found
R78 T232 T231 csubj transforming,is
R79 T233 T234 compound growth,β2
R81 T235 T234 compound factor,β2
R82 T236 T232 dobj signaling,transforming
R85 T239 T231 advcl induce,is
R86 T240 T239 advmod transiently,induce
R88 T242 T239 dobj Snail,induce
R89 T243 T242 compound transcription,Snail
R90 T244 T242 compound factor,Snail
R94 T248 T246 dobj pathway,activate
R95 T249 T250 nmod Ras,kinase
R96 T250 T248 nmod kinase,pathway
R97 T251 T250 punct -,kinase
R98 T252 T253 npadvmod mitogen,activated
R99 T253 T250 amod activated,kinase
R100 T254 T253 punct -,activated
R101 T255 T250 nmod protein,kinase
R102 T256 T250 punct (,kinase
R103 T257 T250 appos MAPK,kinase
R104 T258 T248 punct ),pathway
R125 T282 T290 advcl regulated,released
R126 T283 T284 compound E,cadherin
R128 T285 T284 punct -,cadherin
R129 T286 T282 auxpass is,regulated
R130 T287 T282 advmod transcriptionally,regulated
R131 T288 T282 advmod down,regulated
R132 T289 T282 punct -,regulated
R135 T293 T290 nsubjpass proteins,released
R136 T294 T293 compound adhesion,proteins
R145 T303 T305 compound cell,contacts
R146 T304 T303 punct -,cell
R152 T310 T308 relcl demonstrate,process
R153 T311 T310 nsubj we,demonstrate
R157 T315 T317 compound MAPK,activation
R158 T316 T315 punct -,MAPK
R166 T326 T324 pcomp stimulated,into
R167 T327 T328 amod multipotent,cells
R168 T328 T326 nsubjpass cells,stimulated
R169 T329 T328 prep within,cells
R170 T330 T331 det a,sheet
R171 T331 T329 pobj sheet,within
R172 T332 T326 auxpass are,stimulated
R190 T351 T354 nsubj pathway,weaves
R191 T352 T351 amod novel,pathway
R192 T353 T351 compound signaling,pathway
R202 T364 T361 conj events,morphogens
R203 T365 T364 amod transcriptional,events
R211 T373 T371 pobj skin,within
R212 T374 T373 amod developing,skin
R214 T146 T147 det A,Pathway
R227 T1019 T1020 amod Mammalian,development
R228 T1020 T1021 nsubj development,involves
R229 T1022 T1023 det the,morphogenesis
R230 T1023 T1021 dobj morphogenesis,involves
R231 T1024 T1023 prep of,morphogenesis
R232 T1025 T1026 amod complex,structures
R233 T1026 T1024 pobj structures,of
R234 T1027 T1028 advmod three,dimensional
R235 T1028 T1026 amod dimensional,structures
R236 T1029 T1028 punct -,dimensional
R237 T1030 T1023 prep from,morphogenesis
R238 T1031 T1032 advmod seemingly,uniform
R239 T1032 T1033 amod uniform,sheets
R240 T1033 T1030 pobj sheets,from
R241 T1034 T1033 cc or,sheets
R242 T1035 T1033 conj masses,sheets
R243 T1036 T1033 prep of,sheets
R244 T1037 T1036 pobj cells,of
R245 T1038 T1021 punct .,involves
R246 T1040 T1041 det A,structure
R247 T1041 T1046 nsubj structure,initiates
R248 T1042 T1041 amod simple,structure
R249 T1043 T1044 npadvmod bud,like
R250 T1044 T1041 amod like,structure
R251 T1045 T1044 punct -,like
R252 T1047 T1048 det the,formation
R253 T1048 T1046 dobj formation,initiates
R254 T1049 T1048 prep of,formation
R255 T1050 T1051 amod many,organs
R256 T1051 T1049 pobj organs,of
R257 T1052 T1051 punct ", ",organs
R258 T1053 T1051 prep including,organs
R259 T1054 T1053 pobj lungs,including
R260 T1055 T1054 punct ", ",lungs
R261 T1056 T1057 amod spinal,cord
R262 T1057 T1054 conj cord,lungs
R263 T1058 T1057 punct ", ",cord
R264 T1059 T1060 amod mammary,glands
R265 T1060 T1057 conj glands,cord
R266 T1061 T1060 punct ", ",glands
R267 T1062 T1060 cc and,glands
R268 T1063 T1064 compound hair,follicles
R269 T1064 T1060 conj follicles,glands
R270 T1065 T1066 punct [,1
R271 T1066 T1046 parataxis 1,initiates
R272 T1067 T1066 punct ],1
R273 T1068 T1046 punct .,initiates
R274 T1070 T1071 det The,cells
R275 T1071 T1076 nsubjpass cells,attached
R276 T1072 T1071 amod multipotent,cells
R277 T1073 T1071 punct ", ",cells
R278 T1074 T1071 amod adhering,cells
R279 T1075 T1071 amod epithelial,cells
R280 T1077 T1076 auxpass are,attached
R281 T1078 T1076 advmod typically,attached
R282 T1079 T1076 prep to,attached
R283 T1080 T1081 det an,lamina
R284 T1081 T1079 pobj lamina,to
R285 T1082 T1081 amod underlying,lamina
R286 T1083 T1081 amod basal,lamina
R287 T1084 T1085 dep that,polarizes
R288 T1085 T1081 relcl polarizes,lamina
R289 T1086 T1087 det the,sheet
R290 T1087 T1085 dobj sheet,polarizes
R291 T1088 T1087 amod epithelial,sheet
R292 T1089 T1085 cc and,polarizes
R293 T1090 T1085 conj separates,polarizes
R294 T1091 T1090 dobj it,separates
R295 T1092 T1090 prep from,separates
R296 T1093 T1094 amod surrounding,mesenchyme
R297 T1094 T1092 pobj mesenchyme,from
R298 T1095 T1076 punct .,attached
R299 T1097 T1098 amod Budding,morphogenesis
R300 T1098 T1099 nsubjpass morphogenesis,guided
R301 T1100 T1099 auxpass is,guided
R302 T1101 T1099 agent by,guided
R303 T1102 T1103 det a,exchange
R304 T1103 T1101 pobj exchange,by
R305 T1104 T1103 amod reciprocal,exchange
R306 T1105 T1103 prep of,exchange
R307 T1106 T1105 pobj signals,of
R308 T1107 T1103 prep between,exchange
R309 T1108 T1107 pobj epithelium,between
R310 T1109 T1108 cc and,epithelium
R311 T1110 T1108 conj mesenchyme,epithelium
R312 T1111 T1112 aux to,specify
R313 T1112 T1103 advcl specify,exchange
R314 T1113 T1114 det the,identity
R315 T1114 T1112 dobj identity,specify
R316 T1115 T1114 prep of,identity
R317 T1116 T1117 det the,organ
R318 T1117 T1115 pobj organ,of
R319 T1118 T1119 dep that,form
R320 T1119 T1117 relcl form,organ
R321 T1120 T1119 aux will,form
R322 T1121 T1112 cc and,specify
R323 T1122 T1123 aux to,govern
R324 T1123 T1112 conj govern,specify
R325 T1124 T1125 poss its,growth
R326 T1125 T1123 dobj growth,govern
R327 T1126 T1099 punct .,guided
R328 T1128 T1129 prep At,are
R329 T1130 T1131 det the,helm
R330 T1131 T1128 pobj helm,At
R331 T1132 T1131 prep of,helm
R332 T1133 T1134 det these,pathways
R333 T1134 T1132 pobj pathways,of
R334 T1135 T1136 amod molecular,communication
R335 T1136 T1134 compound communication,pathways
R336 T1137 T1129 nsubj Wnts,are
R337 T1138 T1137 punct ", ",Wnts
R338 T1139 T1140 nmod bone,proteins
R339 T1140 T1137 appos proteins,Wnts
R340 T1141 T1140 amod morphogenic,proteins
R341 T1142 T1140 punct (,proteins
R342 T1143 T1140 appos BMPs,proteins
R343 T1144 T1137 punct ),Wnts
R344 T1145 T1137 punct ", ",Wnts
R345 T1146 T1147 amod transforming,βs
R346 T1147 T1137 appos βs,Wnts
R347 T1148 T1147 compound growth,βs
R348 T1149 T1147 compound factor,βs
R349 T1150 T1147 punct (,βs
R350 T1151 T1152 compound TGF,βs
R351 T1152 T1147 appos βs,βs
R352 T1153 T1152 punct -,βs
R353 T1154 T1137 punct ),Wnts
R354 T1155 T1137 punct ", ",Wnts
R355 T1156 T1137 cc and,Wnts
R356 T1157 T1158 compound fibroblast,factors
R357 T1158 T1137 conj factors,Wnts
R358 T1159 T1158 compound growth,factors
R359 T1160 T1158 punct (,factors
R360 T1161 T1158 appos FGFs,factors
R361 T1162 T1129 punct ),are
R362 T1163 T1129 punct .,are
R363 T1165 T1166 prep Through,trigger
R364 T1167 T1165 pobj activation,Through
R365 T1168 T1167 prep of,activation
R366 T1169 T1170 compound cell,surface
R367 T1170 T1171 compound surface,receptors
R368 T1171 T1168 pobj receptors,of
R369 T1172 T1171 compound transmembrane,receptors
R370 T1173 T1166 punct ", ",trigger
R371 T1174 T1175 det these,molecules
R372 T1175 T1166 nsubj molecules,trigger
R373 T1176 T1175 amod external,molecules
R374 T1177 T1175 compound signaling,molecules
R375 T1178 T1179 amod distinct,cascades
R376 T1179 T1166 dobj cascades,trigger
R377 T1180 T1179 prep of,cascades
R378 T1181 T1182 amod intracellular,events
R379 T1182 T1180 pobj events,of
R380 T1183 T1184 dep that,culminate
R381 T1184 T1179 relcl culminate,cascades
R382 T1185 T1184 prep in,culminate
R383 T1186 T1185 pobj changes,in
R384 T1187 T1186 prep in,changes
R385 T1188 T1189 compound gene,expression
R386 T1189 T1187 pobj expression,in
R387 T1190 T1189 punct ", ",expression
R388 T1191 T1189 conj growth,expression
R389 T1192 T1191 punct ", ",growth
R390 T1193 T1191 cc and,growth
R391 T1194 T1191 conj differentiation,growth
R392 T1195 T1196 punct [,2
R393 T1196 T1166 parataxis 2,trigger
R394 T1197 T1196 punct ],2
R395 T1198 T1166 punct .,trigger
R396 T1200 T1201 advmod How,collaborates
R397 T1201 T1206 csubj collaborates,has
R398 T1202 T1203 det this,constellation
R399 T1203 T1201 nsubj constellation,collaborates
R400 T1204 T1203 prep of,constellation
R401 T1205 T1204 pobj signals,of
R402 T1207 T1201 prep in,collaborates
R403 T1208 T1207 pcomp tailoring,in
R404 T1209 T1210 det each,process
R405 T1210 T1208 dobj process,tailoring
R406 T1211 T1210 compound budding,process
R407 T1212 T1213 mark so,executes
R408 T1213 T1208 advcl executes,tailoring
R409 T1214 T1213 mark that,executes
R410 T1215 T1213 nsubj it,executes
R411 T1216 T1217 det a,program
R412 T1217 T1213 dobj program,executes
R413 T1218 T1217 amod distinct,program
R414 T1219 T1217 amod morphogenetic,program
R415 T1220 T1221 advmod yet,defined
R416 T1221 T1206 xcomp defined,has
R417 T1222 T1221 aux to,defined
R418 T1223 T1221 auxpass be,defined
R419 T1224 T1221 advmod comprehensively,defined
R420 T1225 T1206 punct .,has
R421 T1227 T1228 advmod However,appears
R422 T1229 T1228 punct ", ",appears
R423 T1230 T1231 det the,process
R424 T1231 T1228 nsubj process,appears
R425 T1232 T1233 aux to,patterned
R426 T1233 T1228 xcomp patterned,appears
R427 T1234 T1233 auxpass be,patterned
R428 T1235 T1233 prep at,patterned
R429 T1236 T1237 det the,stages
R430 T1237 T1235 pobj stages,at
R431 T1238 T1237 amod initial,stages
R432 T1239 T1237 prep of,stages
R433 T1240 T1241 compound bud,formation
R434 T1241 T1239 pobj formation,of
R435 T1242 T1228 punct ", ",appears
R436 T1243 T1244 mark since,differ
R437 T1244 T1228 advcl differ,appears
R438 T1245 T1246 det the,importance
R439 T1246 T1244 nsubj importance,differ
R440 T1247 T1246 amod relative,importance
R441 T1248 T1246 prep of,importance
R442 T1249 T1250 det these,pathways
R443 T1250 T1248 pobj pathways,of
R444 T1251 T1250 cc and,pathways
R445 T1252 T1253 poss their,effectors
R446 T1253 T1250 conj effectors,pathways
R447 T1254 T1253 amod downstream,effectors
R448 T1255 T1256 mark as,begin
R449 T1256 T1244 advcl begin,differ
R450 T1257 T1256 nsubj buds,begin
R451 T1258 T1259 aux to,develop
R452 T1259 T1256 xcomp develop,begin
R453 T1260 T1256 cc and,begin
R454 T1261 T1262 compound cell,fates
R455 T1262 T1263 nsubjpass fates,specified
R456 T1263 T1256 conj specified,begin
R457 T1264 T1263 auxpass are,specified
R458 T1265 T1228 punct .,appears
R459 T1267 T1268 det The,development
R460 T1268 T1269 nsubj development,requires
R461 T1270 T1268 prep of,development
R462 T1271 T1272 det a,bud
R463 T1272 T1270 pobj bud,of
R464 T1273 T1274 det a,number
R465 T1274 T1269 dobj number,requires
R466 T1275 T1274 prep of,number
R467 T1276 T1277 amod coordinated,changes
R468 T1277 T1275 pobj changes,of
R469 T1278 T1277 prep in,changes
R470 T1279 T1280 det the,behavior
R471 T1280 T1278 pobj behavior,in
R472 T1281 T1280 prep of,behavior
R473 T1282 T1283 det the,cells
R474 T1283 T1281 pobj cells,of
R475 T1284 T1283 amod targeted,cells
R476 T1285 T1280 prep within,behavior
R477 T1286 T1287 det an,sheet
R478 T1287 T1285 pobj sheet,within
R479 T1288 T1287 amod epithelial,sheet
R480 T1289 T1269 punct .,requires
R481 T1291 T1292 det The,process
R482 T1292 T1293 nsubjpass process,accompanied
R483 T1294 T1293 aux must,accompanied
R484 T1295 T1293 auxpass be,accompanied
R485 T1296 T1293 prep by,accompanied
R486 T1297 T1296 pobj alterations,by
R487 T1298 T1297 prep in,alterations
R488 T1299 T1300 det the,proliferation
R489 T1300 T1298 pobj proliferation,in
R490 T1301 T1300 punct ", ",proliferation
R491 T1302 T1300 conj polarity,proliferation
R492 T1303 T1302 punct ", ",polarity
R493 T1304 T1302 conj shape,polarity
R494 T1305 T1304 punct ", ",shape
R495 T1306 T1304 cc and,shape
R496 T1307 T1304 conj adhesiveness,shape
R497 T1308 T1300 prep of,proliferation
R498 T1309 T1310 amod selected,cells
R499 T1310 T1308 pobj cells,of
R500 T1311 T1296 punct ", ",by
R501 T1312 T1313 advmod as,as
R502 T1313 T1296 cc as,by
R503 T1314 T1313 advmod well,as
R504 T1315 T1296 conj by,by
R505 T1316 T1315 pobj modifications,by
R506 T1317 T1316 prep in,modifications
R507 T1318 T1319 poss their,lamina
R508 T1319 T1317 pobj lamina,in
R509 T1320 T1319 amod underlying,lamina
R510 T1321 T1319 amod basal,lamina
R511 T1322 T1293 punct .,accompanied
R512 T1324 T1325 advmod Thus,orchestrated
R513 T1326 T1325 punct ", ",orchestrated
R514 T1327 T1328 amod extracellular,crosstalk
R515 T1328 T1325 nsubjpass crosstalk,orchestrated
R516 T1329 T1330 amod epithelial,mesenchymal
R517 T1330 T1328 amod mesenchymal,crosstalk
R518 T1331 T1330 punct -,mesenchymal
R519 T1332 T1325 aux must,orchestrated
R520 T1333 T1325 auxpass be,orchestrated
R521 T1334 T1325 advmod intricately,orchestrated
R522 T1335 T1336 aux to,couple
R523 T1336 T1325 advcl couple,orchestrated
R524 T1337 T1338 det the,determination
R525 T1338 T1336 dobj determination,couple
R526 T1339 T1338 prep of,determination
R527 T1340 T1341 amod distinct,fates
R528 T1341 T1339 pobj fates,of
R529 T1342 T1341 compound cell,fates
R530 T1343 T1336 prep with,couple
R531 T1344 T1345 det the,remodeling
R532 T1345 T1343 pobj remodeling,with
R533 T1346 T1345 amod contemporaneous,remodeling
R534 T1347 T1345 prep of,remodeling
R535 T1348 T1349 det the,properties
R536 T1349 T1347 pobj properties,of
R537 T1350 T1349 amod physical,properties
R538 T1351 T1350 cc and,physical
R539 T1352 T1350 conj structural,physical
R540 T1353 T1349 prep of,properties
R541 T1354 T1355 det the,cell
R542 T1355 T1353 pobj cell,of
R543 T1356 T1325 punct .,orchestrated
R544 T1358 T1359 prep Among,offer
R545 T1360 T1361 det the,organs
R546 T1361 T1358 pobj organs,Among
R547 T1362 T1361 amod few,organs
R548 T1363 T1361 amod dispensable,organs
R549 T1364 T1359 punct ", ",offer
R550 T1365 T1366 compound hair,follicles
R551 T1366 T1359 nsubj follicles,offer
R552 T1367 T1368 det an,system
R553 T1368 T1359 dobj system,offer
R554 T1369 T1368 amod excellent,system
R555 T1370 T1368 compound model,system
R556 T1371 T1372 aux to,study
R557 T1372 T1368 advcl study,system
R558 T1373 T1374 amod epithelial,bud
R559 T1374 T1375 compound bud,formation
R560 T1375 T1372 dobj formation,study
R561 T1376 T1359 punct .,offer
R562 T1378 T1379 amod Mammalian,epithelium
R563 T1379 T1381 nsubj epithelium,begins
R564 T1380 T1379 compound skin,epithelium
R565 T1382 T1381 prep as,begins
R566 T1383 T1384 det a,sheet
R567 T1384 T1382 pobj sheet,as
R568 T1385 T1384 amod single,sheet
R569 T1386 T1384 prep of,sheet
R570 T1387 T1388 amod multipotent,cells
R571 T1388 T1386 pobj cells,of
R572 T1389 T1388 amod ectodermal,cells
R573 T1390 T1381 punct .,begins
R574 T1392 T1393 prep During,populate
R575 T1394 T1392 pobj development,During
R576 T1395 T1393 punct ", ",populate
R577 T1396 T1397 amod specialized,cells
R578 T1397 T1393 nsubj cells,populate
R579 T1398 T1397 amod mesenchymal,cells
R580 T1399 T1400 det the,skin
R581 T1400 T1393 dobj skin,populate
R582 T1401 T1393 prep in,populate
R583 T1402 T1403 det a,pattern
R584 T1403 T1401 pobj pattern,in
R585 T1404 T1405 advmod spatially,defined
R586 T1405 T1403 amod defined,pattern
R587 T1406 T1407 aux to,initiate
R588 T1407 T1393 advcl initiate,populate
R589 T1408 T1409 det the,crosstalk
R590 T1409 T1407 dobj crosstalk,initiate
R591 T1410 T1409 amod complex,crosstalk
R592 T1411 T1412 amod epithelial,mesenchymal
R593 T1412 T1409 amod mesenchymal,crosstalk
R594 T1413 T1412 punct -,mesenchymal
R595 T1414 T1415 dep that,specify
R596 T1415 T1409 relcl specify,crosstalk
R597 T1416 T1415 aux will,specify
R598 T1417 T1418 det the,bud
R599 T1418 T1415 dobj bud,specify
R600 T1419 T1420 punct [,3
R601 T1420 T1393 parataxis 3,populate
R602 T1421 T1420 punct ],3
R603 T1422 T1393 punct .,populate
R604 T1424 T1425 mark Once,committed
R605 T1425 T1426 advcl committed,instructs
R606 T1427 T1426 punct ", ",instructs
R607 T1428 T1429 det a,cluster
R608 T1429 T1426 nsubj cluster,instructs
R609 T1430 T1429 amod small,cluster
R610 T1431 T1429 prep of,cluster
R611 T1432 T1433 amod epithelial,cells
R612 T1433 T1431 pobj cells,of
R613 T1434 T1429 punct ", ",cluster
R614 T1435 T1436 det the,placode
R615 T1436 T1429 appos placode,cluster
R616 T1437 T1426 punct ", ",instructs
R617 T1438 T1439 det a,group
R618 T1439 T1440 nsubj group,condense
R619 T1440 T1426 ccomp condense,instructs
R620 T1441 T1439 prep of,group
R621 T1442 T1443 amod underlying,cells
R622 T1443 T1441 pobj cells,of
R623 T1444 T1443 amod mesenchymal,cells
R624 T1445 T1440 aux to,condense
R625 T1446 T1440 cc and,condense
R626 T1447 T1440 conj form,condense
R627 T1448 T1449 det the,papilla
R628 T1449 T1447 dobj papilla,form
R629 T1450 T1449 amod nascent,papilla
R630 T1451 T1449 amod dermal,papilla
R631 T1452 T1449 punct ", ",papilla
R632 T1453 T1454 dep which,be
R633 T1454 T1449 relcl be,papilla
R634 T1455 T1454 aux will,be
R635 T1456 T1457 det a,fixture
R636 T1457 T1454 attr fixture,be
R637 T1458 T1457 amod permanent,fixture
R638 T1459 T1457 prep of,fixture
R639 T1460 T1461 det the,follicle
R640 T1461 T1459 pobj follicle,of
R641 T1462 T1461 compound hair,follicle
R642 T1463 T1426 punct .,instructs
R643 T1465 T1466 amod Subsequent,exchanges
R644 T1466 T1467 nsubj exchanges,result
R645 T1468 T1466 prep between,exchanges
R646 T1469 T1470 det the,placode
R647 T1470 T1468 pobj placode,between
R648 T1471 T1470 cc and,placode
R649 T1472 T1473 amod nascent,papilla
R650 T1473 T1470 conj papilla,placode
R651 T1474 T1473 amod dermal,papilla
R652 T1475 T1467 prep in,result
R653 T1476 T1477 amod further,growth
R654 T1477 T1475 pobj growth,in
R655 T1478 T1477 prep of,growth
R656 T1479 T1480 det the,follicle
R657 T1480 T1478 pobj follicle,of
R658 T1481 T1477 prep into,growth
R659 T1482 T1483 det the,dermis
R660 T1483 T1481 pobj dermis,into
R661 T1484 T1483 amod underlying,dermis
R662 T1485 T1477 punct ", ",growth
R663 T1486 T1477 cc or,growth
R664 T1487 T1488 amod down,growth
R665 T1488 T1477 conj growth,growth
R666 T1489 T1488 punct -,growth
R667 T1490 T1488 punct ", ",growth
R668 T1491 T1488 cc and,growth
R669 T1492 T1493 amod eventual,differentiation
R670 T1493 T1488 conj differentiation,growth
R671 T1494 T1493 prep into,differentiation
R672 T1495 T1496 det the,layers
R673 T1496 T1494 pobj layers,into
R674 T1497 T1496 nummod six,layers
R675 T1498 T1496 amod concentric,layers
R676 T1499 T1496 prep of,layers
R677 T1500 T1501 det the,follicle
R678 T1501 T1499 pobj follicle,of
R679 T1502 T1501 amod mature,follicle
R680 T1503 T1467 punct .,result
R681 T1505 T1506 advmod Previously,delineated
R682 T1507 T1506 punct ", ",delineated
R683 T1508 T1506 nsubj we,delineated
R684 T1509 T1510 advmod how,function
R685 T1510 T1506 ccomp function,delineated
R686 T1511 T1512 nummod two,signals
R687 T1512 T1510 nsubj signals,function
R688 T1513 T1512 amod respective,signals
R689 T1514 T1512 amod epithelial,signals
R690 T1515 T1514 cc and,epithelial
R691 T1516 T1514 conj mesenchymal,epithelial
R692 T1517 T1512 punct ", ",signals
R693 T1518 T1512 appos Wnts,signals
R694 T1519 T1518 cc and,Wnts
R695 T1520 T1521 det the,factor
R696 T1521 T1518 conj factor,Wnts
R697 T1522 T1523 npadvmod BMP,inhibitory
R698 T1523 T1521 amod inhibitory,factor
R699 T1524 T1523 punct -,inhibitory
R700 T1525 T1521 appos noggin,factor
R701 T1526 T1510 punct ", ",function
R702 T1527 T1510 prep in,function
R703 T1528 T1527 pobj concert,in
R704 T1529 T1530 aux to,induce
R705 T1530 T1510 advcl induce,function
R706 T1531 T1532 amod lymphoid,factor
R707 T1532 T1534 npadvmod factor,mediated
R708 T1533 T1532 compound enhancer,factor
R709 T1534 T1551 amod mediated,transcription
R710 T1535 T1532 punct -,factor
R711 T1536 T1532 nummod 1,factor
R712 T1537 T1532 punct /,factor
R713 T1538 T1539 compound β,catenin
R714 T1539 T1532 appos catenin,factor
R715 T1540 T1539 punct -,catenin
R716 T1541 T1532 punct (,factor
R717 T1542 T1532 appos LEF,factor
R718 T1543 T1542 punct -,LEF
R719 T1544 T1542 nummod 1,LEF
R720 T1545 T1542 punct /,LEF
R721 T1546 T1547 compound β,catenin
R722 T1547 T1542 appos catenin,LEF
R723 T1548 T1547 punct -,catenin
R724 T1549 T1534 punct ),mediated
R725 T1550 T1534 punct -,mediated
R726 T1551 T1530 dobj transcription,induce
R727 T1552 T1551 compound gene,transcription
R728 T1553 T1530 prep within,induce
R729 T1554 T1555 det the,placode
R730 T1555 T1553 pobj placode,within
R731 T1556 T1555 compound follicle,placode
R732 T1557 T1558 punct [,4
R733 T1558 T1506 parataxis 4,delineated
R734 T1559 T1558 punct ],4
R735 T1560 T1506 punct .,delineated
R736 T1562 T1563 det The,changes
R737 T1563 T1565 nsubj changes,include
R738 T1564 T1563 amod downstream,changes
R739 T1566 T1563 acl elicited,changes
R740 T1567 T1566 prep through,elicited
R741 T1568 T1567 pobj convergence,through
R742 T1569 T1568 prep of,convergence
R743 T1675 T1677 compound cell,contact
R744 T1676 T1675 punct -,cell
R745 T1677 T1673 pobj contact,of
R746 T1678 T1671 punct ", ",recruit
R747 T1679 T1671 advmod however,recruit
R748 T1570 T1571 det these,pathways
R749 T1680 T1671 punct ", ",recruit
R750 T1571 T1569 pobj pathways,of
R751 T1681 T1682 nmod E,cadherin
R752 T1572 T1571 nummod two,pathways
R753 T1573 T1571 amod early,pathways
R754 T1682 T1684 nmod cadherin,complexes
R755 T1574 T1571 compound signaling,pathways
R756 T1575 T1576 amod down,regulation
R757 T1683 T1682 punct -,cadherin
R758 T1576 T1565 dobj regulation,include
R759 T1577 T1576 punct -,regulation
R760 T1578 T1576 prep of,regulation
R761 T1684 T1671 nsubj complexes,recruit
R762 T1579 T1580 det the,gene
R763 T1580 T1578 pobj gene,of
R764 T1685 T1682 punct -,cadherin
R765 T1581 T1580 acl encoding,gene
R766 T1582 T1583 compound E,cadherin
R767 T1583 T1581 dobj cadherin,encoding
R768 T1686 T1687 compound β,catenin
R769 T1584 T1583 punct -,cadherin
R770 T1585 T1583 punct ", ",cadherin
R771 T1586 T1587 det the,cadherin
R772 T1687 T1682 appos catenin,cadherin
R773 T1587 T1583 appos cadherin,cadherin
R774 T1588 T1587 amod prototypical,cadherin
R775 T1688 T1687 punct -,catenin
R776 T1589 T1587 amod epithelial,cadherin
R777 T1590 T1591 dep that,forms
R778 T1591 T1587 relcl forms,cadherin
R779 T1689 T1690 compound α,catenin
R780 T1592 T1593 det the,core
R781 T1593 T1591 dobj core,forms
R782 T1690 T1671 dobj catenin,recruit
R783 T1594 T1593 amod transmembrane,core
R784 T1595 T1593 prep of,core
R785 T1596 T1597 amod intercellular,junctions
R786 T1691 T1690 punct -,catenin
R787 T1597 T1595 pobj junctions,of
R788 T1598 T1597 compound adherens,junctions
R789 T1599 T1597 punct (,junctions
R790 T1692 T1690 punct ", ",catenin
R791 T1600 T1597 appos AJs,junctions
R792 T1601 T1565 punct ),include
R793 T1602 T1603 punct [,5
R794 T1603 T1565 parataxis 5,include
R795 T1604 T1603 punct ],5
R796 T1693 T1694 dep which,coordinates
R797 T1605 T1565 punct .,include
R798 T1607 T1608 nsubj We,showed
R799 T1694 T1690 relcl coordinates,catenin
R800 T1609 T1608 advmod subsequently,showed
R801 T1695 T1694 prep in,coordinates
R802 T1610 T1611 mark that,blocked
R803 T1696 T1695 pobj turn,in
R804 T1611 T1608 ccomp blocked,showed
R805 T1697 T1698 det the,dynamics
R806 T1612 T1613 advmod when,elevated
R807 T1613 T1611 advcl elevated,blocked
R808 T1698 T1694 dobj dynamics,coordinates
R809 T1614 T1615 compound E,cadherin
R810 T1615 T1613 nsubjpass cadherin,elevated
R811 T1616 T1615 punct -,cadherin
R812 T1699 T1698 amod associated,dynamics
R813 T1617 T1613 auxpass is,elevated
R814 T1618 T1613 advmod transgenically,elevated
R815 T1700 T1698 compound actin,dynamics
R816 T1619 T1613 prep in,elevated
R817 T1620 T1621 compound mouse,skin
R818 T1621 T1619 pobj skin,in
R819 T1701 T1698 compound polymerization,dynamics
R820 T1622 T1611 punct ", ",blocked
R821 T1623 T1624 compound hair,follicle
R822 T1624 T1625 compound follicle,morphogenesis
R823 T1702 T1698 amod necessary,dynamics
R824 T1625 T1611 nsubjpass morphogenesis,blocked
R825 T1626 T1611 auxpass is,blocked
R826 T1627 T1611 punct ", ",blocked
R827 T1703 T1704 aux to,stabilize
R828 T1628 T1611 advcl suggesting,blocked
R829 T1629 T1630 mark that,is
R830 T1630 T1628 ccomp is,suggesting
R831 T1704 T1702 xcomp stabilize,necessary
R832 T1631 T1632 nmod E,cadherin
R833 T1632 T1634 nmod cadherin,regulation
R834 T1705 T1706 amod nascent,AJs
R835 T1633 T1632 punct -,cadherin
R836 T1634 T1630 nsubj regulation,is
R837 T1635 T1634 amod down,regulation
R838 T1706 T1704 dobj AJs,stabilize
R839 T1636 T1634 punct -,regulation
R840 T1637 T1638 det a,event
R841 T1638 T1630 attr event,is
R842 T1639 T1638 amod critical,event
R843 T1640 T1638 prep in,event
R844 T1707 T1704 cc and,stabilize
R845 T1708 T1704 conj integrate,stabilize
R846 T1641 T1640 pcomp governing,in
R847 T1709 T1710 det the,cytoskeleton
R848 T1642 T1643 det the,dynamics
R849 T1643 T1641 dobj dynamics,governing
R850 T1644 T1643 compound adhesion,dynamics
R851 T1710 T1708 dobj cytoskeleton,integrate
R852 T1645 T1643 amod necessary,dynamics
R853 T1646 T1645 prep for,necessary
R854 T1647 T1648 compound budding,morphogenesis
R855 T1711 T1708 prep across,integrate
R856 T1648 T1646 pobj morphogenesis,for
R857 T1649 T1650 punct [,4
R858 T1712 T1713 det an,sheet
R859 T1650 T1608 parataxis 4,showed
R860 T1651 T1650 punct ],4
R861 T1652 T1608 punct .,showed
R862 T1713 T1711 pobj sheet,across
R863 T1654 T1655 prep Like,binds
R864 T1714 T1713 amod epithelial,sheet
R865 T1656 T1654 pobj LEF,Like
R866 T1657 T1656 punct -,LEF
R867 T1715 T1716 punct [,8
R868 T1658 T1656 nummod 1,LEF
R869 T1659 T1655 punct ", ",binds
R870 T1660 T1661 compound E,cadherin
R871 T1661 T1655 nsubj cadherin,binds
R872 T1716 T1694 parataxis 8,coordinates
R873 T1662 T1661 punct -,cadherin
R874 T1663 T1655 advmod also,binds
R875 T1717 T1716 nummod 6,8
R876 T1664 T1655 prep to,binds
R877 T1665 T1666 compound β,catenin
R878 T1666 T1664 pobj catenin,to
R879 T1718 T1716 punct ",",8
R880 T1667 T1666 punct -,catenin
R881 T1668 T1655 punct .,binds
R882 T1719 T1716 nummod 7,8
R883 T1670 T1671 prep At,recruit
R884 T1720 T1716 punct ",",8
R885 T1672 T1670 pobj sites,At
R886 T1673 T1672 prep of,sites
R887 T1674 T1675 compound cell,cell
R888 T1721 T1716 punct ],8
R889 T1722 T1671 punct .,recruit
R890 T1724 T1725 compound α,Catenin
R891 T1725 T1727 nsubj Catenin,binds
R892 T1726 T1725 punct -,Catenin
R893 T1780 T1779 compound protein,kinase
R894 T1781 T1779 punct (,kinase
R895 T1782 T1779 appos MAPK,kinase
R896 T1728 T1727 advmod also,binds
R897 T1783 T1773 punct ),pathway
R898 T1784 T1785 punct [,10
R899 T1729 T1727 prep to,binds
R900 T1785 T1727 parataxis 10,binds
R901 T1786 T1785 nummod 9,10
R902 T1730 T1731 det the,protein
R903 T1787 T1785 punct ",",10
R904 T1788 T1785 punct ],10
R905 T1731 T1729 pobj protein,to
R906 T1789 T1727 punct .,binds
R907 T1732 T1731 nmod class,protein
R908 T1791 T1792 prep Through,appear
R909 T1793 T1794 det these,links
R910 T1733 T1732 nummod III,class
R911 T1794 T1791 pobj links,Through
R912 T1795 T1792 punct ", ",appear
R913 T1734 T1731 nmod Lin,protein
R914 T1796 T1792 nsubj AJs,appear
R915 T1797 T1792 oprd able,appear
R916 T1798 T1799 aux to,couple
R917 T1735 T1734 punct -,Lin
R918 T1799 T1797 xcomp couple,able
R919 T1800 T1799 dobj adhesion,couple
R920 T1736 T1734 nummod 1,Lin
R921 T1801 T1799 prep with,couple
R922 T1802 T1803 amod cytoskeletal,dynamics
R923 T1803 T1801 pobj dynamics,with
R924 T1737 T1734 punct ", ",Lin
R925 T1804 T1805 advmod as,as
R926 T1805 T1801 cc as,with
R927 T1806 T1805 advmod well,as
R928 T1738 T1734 appos Isl,Lin
R929 T1807 T1801 conj with,with
R930 T1808 T1809 amod nuclear,signaling
R931 T1739 T1738 punct -,Isl
R932 T1809 T1807 pobj signaling,with
R933 T1810 T1808 cc and,nuclear
R934 T1811 T1808 conj cytoplasmic,nuclear
R935 T1740 T1738 nummod 1,Isl
R936 T1812 T1792 punct .,appear
R937 T1814 T1815 nsubj This,provides
R938 T1741 T1734 punct ", ",Lin
R939 T1816 T1817 det a,framework
R940 T1742 T1734 appos Mec,Lin
R941 T1817 T1815 dobj framework,provides
R942 T1818 T1817 prep for,framework
R943 T1819 T1818 pcomp conceptualizing,for
R944 T1820 T1821 advmod why,appear
R945 T1821 T1819 ccomp appear,conceptualizing
R946 T1822 T1823 compound E,cadherin
R947 T1743 T1742 punct -,Mec
R948 T1823 T1825 compound cadherin,levels
R949 T1824 T1823 punct -,cadherin
R950 T1825 T1821 nsubj levels,appear
R951 T1826 T1827 aux to,impact
R952 T1744 T1742 nummod 3,Mec
R953 T1827 T1821 xcomp impact,appear
R954 T1828 T1827 prep upon,impact
R955 T1745 T1734 punct (,Lin
R956 T1829 T1830 det a,plethora
R957 T1830 T1828 pobj plethora,upon
R958 T1831 T1830 prep of,plethora
R959 T1746 T1734 appos LIM,Lin
R960 T1832 T1833 amod developmental,processes
R961 T1833 T1831 pobj processes,of
R962 T1834 T1835 punct (,reviewed
R963 T1747 T1731 punct ),protein
R964 T1835 T1815 parataxis reviewed,provides
R965 T1836 T1835 prep in,reviewed
R966 T1837 T1836 punct [,in
R967 T1748 T1731 appos Ajuba,protein
R968 T1838 T1836 pobj 11,in
R969 T1839 T1835 punct ],reviewed
R970 T1840 T1835 punct ),reviewed
R971 T1749 T1731 punct (,protein
R972 T1841 T1815 punct .,provides
R973 T1750 T1751 det a,member
R974 T1843 T1844 mark As,probed
R975 T1844 T1846 advcl probed,intrigued
R976 T1845 T1844 nsubj we,probed
R977 T1751 T1731 appos member,protein
R978 T1847 T1848 advmod more,deeply
R979 T1848 T1844 advmod deeply,probed
R980 T1752 T1751 prep of,member
R981 T1849 T1844 prep into,probed
R982 T1850 T1851 det the,mechanisms
R983 T1851 T1849 pobj mechanisms,into
R984 T1753 T1754 det the,family
R985 T1852 T1851 amod underlying,mechanisms
R986 T1853 T1851 acl governing,mechanisms
R987 T1854 T1855 compound E,cadherin
R988 T1754 T1752 pobj family,of
R989 T1855 T1857 compound cadherin,promoter
R990 T1856 T1855 punct -,cadherin
R991 T1857 T1858 compound promoter,activity
R992 T1755 T1754 compound zyxin,family
R993 T1858 T1853 dobj activity,governing
R994 T1859 T1846 punct ", ",intrigued
R995 T1756 T1754 prep of,family
R996 T1860 T1846 nsubjpass we,intrigued
R997 T1861 T1846 auxpass were,intrigued
R998 T1862 T1846 agent by,intrigued
R999 T1863 T1864 det the,proximity
R1000 T1864 T1862 pobj proximity,by
R1001 T1865 T1864 amod close,proximity
R1002 T1757 T1756 pobj proteins,of
R1003 T1866 T1864 prep of,proximity
R1004 T1867 T1868 det the,site
R1005 T1758 T1731 punct ),protein
R1006 T1759 T1731 punct ", ",protein
R1007 T1868 T1866 pobj site,of
R1008 T1869 T1868 nmod LEF,site
R1009 T1870 T1869 punct -,LEF
R1010 T1760 T1761 dep which,appears
R1011 T1871 T1869 nummod 1,LEF
R1012 T1872 T1869 punct /,LEF
R1013 T1761 T1731 relcl appears,protein
R1014 T1873 T1874 compound β,catenin
R1015 T1762 T1763 aux to,function
R1016 T1874 T1869 appos catenin,LEF
R1017 T1875 T1874 punct -,catenin
R1018 T1876 T1868 compound binding,site
R1019 T1763 T1761 xcomp function,appears
R1020 T1877 T1864 prep to,proximity
R1021 T1878 T1879 det a,site
R1022 T1879 T1877 pobj site,to
R1023 T1764 T1763 advmod dually,function
R1024 T1880 T1879 acl known,site
R1025 T1881 T1882 aux to,bind
R1026 T1765 T1763 prep in,function
R1027 T1882 T1880 xcomp bind,known
R1028 T1883 T1884 det the,family
R1029 T1884 T1882 dobj family,bind
R1030 T1766 T1765 preconj both,in
R1031 T1767 T1765 conj adhesion,in
R1032 T1768 T1765 cc and,in
R1033 T1769 T1765 conj in,in
R1034 T1885 T1886 compound Snail,Slug
R1035 T1886 T1884 compound Slug,family
R1036 T1770 T1769 pobj activation,in
R1037 T1887 T1886 punct /,Slug
R1038 T1888 T1884 prep of,family
R1039 T1889 T1890 nmod zinc,finger
R1040 T1771 T1770 prep of,activation
R1041 T1890 T1891 nmod finger,proteins
R1042 T1891 T1888 pobj proteins,of
R1043 T1772 T1773 det the,pathway
R1044 T1892 T1893 amod transcriptional,repressor
R1045 T1893 T1891 compound repressor,proteins
R1046 T1894 T1895 punct [,15
R1047 T1773 T1771 pobj pathway,of
R1048 T1895 T1846 parataxis 15,intrigued
R1049 T1896 T1895 nummod 12,15
R1050 T1897 T1895 punct ",",15
R1051 T1898 T1895 nummod 13,15
R1052 T1899 T1895 punct ",",15
R1053 T1900 T1895 nummod 14,15
R1054 T1774 T1773 nmod Ras,pathway
R1055 T1901 T1895 punct ",",15
R1056 T1902 T1895 punct ],15
R1057 T1903 T1846 punct .,intrigued
R1058 T1775 T1774 punct -,Ras
R1059 T1905 T1906 preconj Both,activity
R1060 T1776 T1777 npadvmod mitogen,activated
R1061 T1906 T1907 nsubj activity,play
R1062 T1908 T1906 prep of,activity
R1063 T1777 T1779 amod activated,kinase
R1064 T1909 T1908 pobj Snail,of
R1065 T1910 T1906 cc and,activity
R1066 T1911 T1912 compound down,regulation
R1067 T1778 T1777 punct -,activated
R1068 T1912 T1906 conj regulation,activity
R1069 T1913 T1912 punct -,regulation
R1070 T1914 T1912 prep of,regulation
R1071 T1779 T1774 appos kinase,Ras
R1072 T1915 T1916 compound E,cadherin
R1073 T1916 T1914 pobj cadherin,of
R1074 T1991 T1988 acl tune,ability
R1075 T1917 T1916 punct -,cadherin
R1076 T1918 T1919 amod pivotal,roles
R1077 T1919 T1907 dobj roles,play
R1078 T1920 T1907 prep in,play
R1079 T1992 T1991 advmod fine,tune
R1080 T1921 T1922 amod epithelial,transitions
R1081 T1922 T1920 pobj transitions,in
R1082 T1923 T1921 prep to,epithelial
R1083 T1993 T1991 punct -,tune
R1084 T1924 T1923 amod mesenchymal,to
R1085 T1925 T1922 punct (,transitions
R1086 T1994 T1995 compound cadherin,expression
R1087 T1926 T1922 appos EMTs,transitions
R1088 T1927 T1922 punct ),transitions
R1089 T1928 T1922 punct ", ",transitions
R1090 T1995 T1991 dobj expression,tune
R1091 T1929 T1922 acl typified,transitions
R1092 T1930 T1929 agent by,typified
R1093 T1931 T1932 det the,transformation
R1094 T1996 T1991 prep at,tune
R1095 T1932 T1930 pobj transformation,by
R1096 T1933 T1932 prep of,transformation
R1097 T1934 T1935 amod polarized,cells
R1098 T1997 T1998 det the,level
R1099 T1935 T1933 pobj cells,of
R1100 T1936 T1935 punct ", ",cells
R1101 T1937 T1935 amod adhering,cells
R1102 T1938 T1935 amod epithelial,cells
R1103 T1939 T1932 prep into,transformation
R1104 T1998 T1996 pobj level,at
R1105 T1940 T1941 amod motile,cells
R1106 T1941 T1939 pobj cells,into
R1107 T1999 T1998 amod transcriptional,level
R1108 T1942 T1941 amod mesenchymal,cells
R1109 T1943 T1944 punct [,17
R1110 T1944 T1907 parataxis 17,play
R1111 T2000 T1985 punct ", ",seems
R1112 T1945 T1944 nummod 16,17
R1113 T1946 T1944 punct ",",17
R1114 T1947 T1944 punct ],17
R1115 T2001 T1985 nsubj Snail,seems
R1116 T1948 T1907 punct .,play
R1117 T1950 T1951 compound Bud,formation
R1118 T2002 T2003 aux to,have
R1119 T1951 T1952 nsubj formation,differs
R1120 T2003 T1985 xcomp have,seems
R1121 T1953 T1952 prep from,differs
R1122 T1954 T1955 det an,EMT
R1123 T2004 T2005 det an,effect
R1124 T1955 T1953 pobj EMT,from
R1125 T1956 T1957 mark in,needs
R1126 T2005 T2003 dobj effect,have
R1127 T2006 T2005 amod additive,effect
R1128 T1957 T1952 advcl needs,differs
R1129 T1958 T1957 mark that,needs
R1130 T1959 T1960 compound E,cadherin
R1131 T2007 T2003 prep with,have
R1132 T1960 T1962 compound cadherin,activity
R1133 T1961 T1960 punct -,cadherin
R1134 T1962 T1957 nsubj activity,needs
R1135 T2008 T2007 pobj LEF,with
R1136 T1963 T1964 aux to,regulated
R1137 T1964 T1957 xcomp regulated,needs
R1138 T1965 T1964 aux be,regulated
R1139 T2009 T2008 punct -,LEF
R1140 T1966 T1964 advmod down,regulated
R1141 T1967 T1964 punct -,regulated
R1142 T2010 T2008 nummod 1,LEF
R1143 T1968 T1964 cc but,regulated
R1144 T1969 T1968 neg not,but
R1145 T2011 T2008 punct /,LEF
R1146 T1970 T1964 conj prevented,regulated
R1147 T1971 T1964 punct ", ",regulated
R1148 T1972 T1973 mark so,remodeled
R1149 T2012 T2013 compound β,catenin
R1150 T1973 T1964 advcl remodeled,regulated
R1151 T1974 T1973 mark that,remodeled
R1152 T2013 T2008 appos catenin,LEF
R1153 T1975 T1976 amod adhesive,junctions
R1154 T1976 T1973 nsubjpass junctions,remodeled
R1155 T1977 T1973 auxpass are,remodeled
R1156 T1978 T1979 advmod rather,than
R1157 T1979 T1973 cc than,remodeled
R1158 T1980 T1981 advmod quantitatively,impaired
R1159 T1981 T1973 conj impaired,remodeled
R1160 T2014 T2013 punct -,catenin
R1161 T1982 T1952 punct .,differs
R1162 T2015 T2003 prep in,have
R1163 T1984 T1985 advcl Supportive,seems
R1164 T1986 T1984 prep of,Supportive
R1165 T2016 T2017 advmod negatively,modulating
R1166 T1987 T1988 det an,ability
R1167 T1988 T1986 pobj ability,of
R1168 T1989 T1988 amod underlying,ability
R1169 T2017 T2015 pcomp modulating,in
R1170 T1990 T1991 aux to,tune
R1171 T2018 T2019 compound E,cadherin
R1172 T2019 T2021 compound cadherin,promoter
R1173 T2020 T2019 punct -,cadherin
R1174 T2021 T2022 compound promoter,activity
R1175 T2022 T2017 dobj activity,modulating
R1176 T2023 T2024 punct [,4
R1177 T2097 T2096 acl associated,features
R1178 T2098 T2097 prep with,associated
R1179 T2024 T1985 parataxis 4,seems
R1180 T2099 T2100 compound hair,bud
R1181 T2100 T2101 compound bud,formation
R1182 T2025 T2024 punct ],4
R1183 T2101 T2098 pobj formation,with
R1184 T2102 T2096 punct ", ",features
R1185 T2103 T2096 prep including,features
R1186 T2026 T1985 punct .,seems
R1187 T2104 T2105 compound down,regulation
R1188 T2105 T2103 pobj regulation,including
R1189 T2028 T2029 prep In,discovered
R1190 T2106 T2105 punct -,regulation
R1191 T2107 T2105 prep of,regulation
R1192 T2108 T2109 compound E,cadherin
R1193 T2109 T2107 pobj cadherin,of
R1194 T2030 T2031 det the,study
R1195 T2110 T2109 punct -,cadherin
R1196 T2111 T2105 punct ", ",regulation
R1197 T2112 T2113 amod increased,proliferation
R1198 T2113 T2105 conj proliferation,regulation
R1199 T2031 T2028 pobj study,In
R1200 T2114 T2113 punct ", ",proliferation
R1201 T2115 T2113 cc and,proliferation
R1202 T2032 T2031 amod present,study
R1203 T2116 T2117 amod repressed,differentiation
R1204 T2117 T2113 conj differentiation,proliferation
R1205 T2118 T2117 amod terminal,differentiation
R1206 T2119 T2084 punct .,appears
R1207 T2033 T2029 punct ", ",discovered
R1208 T2121 T2122 mark Although,reflected
R1209 T2034 T2029 nsubj we,discovered
R1210 T2122 T2133 advcl reflected,appear
R1211 T2123 T2124 det the,spike
R1212 T2035 T2036 mark that,expressed
R1213 T2124 T2122 nsubjpass spike,reflected
R1214 T2125 T2124 amod temporal,spike
R1215 T2036 T2029 ccomp expressed,discovered
R1216 T2126 T2124 prep of,spike
R1217 T2127 T2126 pobj Snail,of
R1218 T2037 T2036 nsubjpass Snail,expressed
R1219 T2128 T2124 prep in,spike
R1220 T2129 T2130 det the,bud
R1221 T2130 T2128 pobj bud,in
R1222 T2038 T2036 auxpass is,expressed
R1223 T2131 T2130 compound hair,bud
R1224 T2132 T2122 auxpass is,reflected
R1225 T2039 T2036 advmod briefly,expressed
R1226 T2134 T2122 prep at,reflected
R1227 T2135 T2136 det the,level
R1228 T2136 T2134 pobj level,at
R1229 T2040 T2036 prep at,expressed
R1230 T2137 T2136 compound mRNA,level
R1231 T2138 T2122 cc and,reflected
R1232 T2139 T2122 conj seems,reflected
R1233 T2041 T2042 det an,stage
R1234 T2140 T2141 aux to,follow
R1235 T2141 T2139 xcomp follow,seems
R1236 T2142 T2143 compound Wnt,signaling
R1237 T2042 T2040 pobj stage,at
R1238 T2143 T2141 dobj signaling,follow
R1239 T2144 T2143 cc and,signaling
R1240 T2043 T2042 amod early,stage
R1241 T2145 T2146 compound BMP,inhibition
R1242 T2146 T2143 conj inhibition,signaling
R1243 T2147 T2133 punct ", ",appear
R1244 T2044 T2042 prep of,stage
R1245 T2148 T2149 nmod LEF,activation
R1246 T2149 T2133 nsubj activation,appear
R1247 T2045 T2046 compound hair,bud
R1248 T2150 T2148 punct -,LEF
R1249 T2151 T2148 nummod 1,LEF
R1250 T2152 T2148 punct /,LEF
R1251 T2046 T2047 compound bud,formation
R1252 T2153 T2154 compound β,catenin
R1253 T2154 T2148 appos catenin,LEF
R1254 T2155 T2154 punct -,catenin
R1255 T2047 T2044 pobj formation,of
R1256 T2156 T2133 aux does,appear
R1257 T2157 T2133 neg not,appear
R1258 T2158 T2159 aux to,induce
R1259 T2159 T2133 xcomp induce,appear
R1260 T2160 T2161 compound Snail,expression
R1261 T2161 T2159 dobj expression,induce
R1262 T2162 T2161 compound gene,expression
R1263 T2048 T2042 punct ", ",stage
R1264 T2163 T2159 prep in,induce
R1265 T2164 T2165 amod embryonic,keratinocytes
R1266 T2165 T2163 pobj keratinocytes,in
R1267 T2166 T2165 compound skin,keratinocytes
R1268 T2049 T2050 advmod when,take
R1269 T2167 T2133 punct .,appear
R1270 T2050 T2042 relcl take,stage
R1271 T2169 T2170 prep In,provide
R1272 T2171 T2169 pobj contrast,In
R1273 T2051 T2052 compound E,cadherin
R1274 T2172 T2170 punct ", ",provide
R1275 T2173 T2170 nsubj we,provide
R1276 T2174 T2175 advmod in,vitro
R1277 T2052 T2050 nsubj cadherin,take
R1278 T2175 T2176 amod vitro,evidence
R1279 T2176 T2170 dobj evidence,provide
R1280 T2053 T2052 punct -,cadherin
R1281 T2177 T2175 punct ", ",vitro
R1282 T2178 T2175 conj transgenic,vitro
R1283 T2179 T2180 punct (,Tg
R1284 T2054 T2055 amod down,regulation
R1285 T2180 T2178 parataxis Tg,transgenic
R1286 T2181 T2180 punct ),Tg
R1287 T2182 T2178 punct ", ",transgenic
R1288 T2055 T2052 appos regulation,cadherin
R1289 T2183 T2178 cc and,transgenic
R1290 T2184 T2185 npadvmod gene,targeting
R1291 T2056 T2055 punct -,regulation
R1292 T2185 T2178 conj targeting,transgenic
R1293 T2186 T2187 aux to,show
R1294 T2057 T2055 cc and,regulation
R1295 T2187 T2170 advcl show,provide
R1296 T2188 T2189 mark that,are
R1297 T2058 T2055 conj activation,regulation
R1298 T2059 T2058 prep of,activation
R1299 T2189 T2187 ccomp are,show
R1300 T2190 T2191 nmod TGF,β2
R1301 T2060 T2059 pobj proliferation,of
R1302 T2191 T2193 nmod β2,signaling
R1303 T2192 T2191 punct -,β2
R1304 T2193 T2189 nsubj signaling,are
R1305 T2061 T2050 dobj place,take
R1306 T2194 T2191 cc and,β2
R1307 T2195 T2196 amod small,phenotype
R1308 T2196 T2197 npadvmod phenotype,related
R1309 T2197 T2204 amod related,protein
R1310 T2198 T2196 punct –,phenotype
R1311 T2199 T2196 cc and,phenotype
R1312 T2062 T2029 punct .,discovered
R1313 T2200 T2196 conj mothers,phenotype
R1314 T2201 T2200 prep against,mothers
R1315 T2202 T2201 pobj decapentaplegic,against
R1316 T2064 T2065 advmod Thereafter,disappears
R1317 T2066 T2065 punct ", ",disappears
R1318 T2203 T2197 punct –,related
R1319 T2204 T2191 conj protein,β2
R1320 T2067 T2065 nsubj Snail,disappears
R1321 T2205 T2204 nummod 2,protein
R1322 T2206 T2204 punct (,protein
R1323 T2207 T2204 appos SMAD2,protein
R1324 T2068 T2065 cc and,disappears
R1325 T2208 T2193 punct ),signaling
R1326 T2209 T2210 amod upstream,inducers
R1327 T2069 T2065 conj remains,disappears
R1328 T2210 T2189 attr inducers,are
R1329 T2211 T2210 prep of,inducers
R1330 T2212 T2213 compound Snail,expression
R1331 T2070 T2069 acomp absent,remains
R1332 T2213 T2211 pobj expression,of
R1333 T2214 T2213 compound gene,expression
R1334 T2071 T2069 prep during,remains
R1335 T2215 T2189 prep in,are
R1336 T2216 T2217 compound skin,epithelium
R1337 T2072 T2073 amod subsequent,growth
R1338 T2217 T2215 pobj epithelium,in
R1339 T2218 T2170 punct .,provide
R1340 T2073 T2071 pobj growth,during
R1341 T2220 T2221 prep In,form
R1342 T2074 T2073 nmod follicle,growth
R1343 T2222 T2223 det the,absence
R1344 T2223 T2220 pobj absence,In
R1345 T2075 T2073 amod down,growth
R1346 T2224 T2223 prep of,absence
R1347 T2225 T2226 compound TGF,β2
R1348 T2226 T2228 compound β2,signaling
R1349 T2076 T2073 punct -,growth
R1350 T2227 T2226 punct -,β2
R1351 T2077 T2073 cc and,growth
R1352 T2228 T2224 pobj signaling,of
R1353 T2229 T2228 cc and,signaling
R1354 T2230 T2231 compound Snail,expression
R1355 T2078 T2073 conj maturation,growth
R1356 T2231 T2228 conj expression,signaling
R1357 T2232 T2231 compound gene,expression
R1358 T2079 T2065 punct .,disappears
R1359 T2233 T2221 punct ", ",form
R1360 T2234 T2235 compound hair,placodes
R1361 T2235 T2221 nsubj placodes,form
R1362 T2236 T2221 aux can,form
R1363 T2237 T2221 punct ", ",form
R1364 T2238 T2221 cc but,form
R1365 T2081 T2082 det This,pattern
R1366 T2239 T2240 amod further,growth
R1367 T2240 T2244 nsubjpass growth,blocked
R1370 T2082 T2084 nsubj pattern,appears
R1371 T2243 T2240 punct -,growth
R1372 T2244 T2221 conj blocked,form
R1373 T2245 T2244 auxpass is,blocked
R1374 T2083 T2082 amod exquisite,pattern
R1375 T2246 T2221 punct .,form
R1376 T2085 T2086 aux to,be
R1377 T2248 T2249 poss Our,studies
R1378 T2249 T2250 nsubj studies,point
R1379 T2251 T2250 prep to,point
R1380 T2086 T2084 xcomp be,appears
R1381 T2252 T2253 det the,view
R1382 T2253 T2251 pobj view,to
R1383 T2087 T2088 advmod functionally,relevant
R1384 T2254 T2255 mark that,functions
R1385 T2255 T2253 acl functions,view
R1386 T2256 T2255 nsubj Snail,functions
R1387 T2088 T2086 acomp relevant,be
R1388 T2257 T2255 advmod likely,functions
R1389 T2258 T2255 advmod downstream,functions
R1390 T2259 T2258 prep of,downstream
R1391 T2089 T2090 mark since,affects
R1392 T2260 T2261 compound cell,fate
R1393 T2261 T2262 compound fate,specification
R1394 T2090 T2086 advcl affects,be
R1395 T2262 T2259 pobj specification,of
R1396 T2263 T2250 punct ", ",point
R1397 T2264 T2250 prep at,point
R1398 T2091 T2090 csubj altering,affects
R1399 T2265 T2266 det a,stage
R1400 T2266 T2264 pobj stage,at
R1401 T2092 T2091 dobj it,altering
R1402 T2267 T2268 advmod where,begins
R1403 T2268 T2266 relcl begins,stage
R1404 T2093 T2094 advmod in,vivo
R1405 T2269 T2270 det the,bud
R1406 T2270 T2268 nsubj bud,begins
R1407 T2094 T2091 advmod vivo,altering
R1408 T2271 T2272 aux to,exhibit
R1409 T2272 T2268 xcomp exhibit,begins
R1410 T2273 T2274 amod enhanced,proliferation
R1411 T2095 T2090 advmod correspondingly,affects
R1412 T2274 T2272 dobj proliferation,exhibit
R1413 T2275 T2274 cc and,proliferation
R1414 T2276 T2274 conj migration,proliferation
R1415 T2277 T2250 punct .,point
R1416 T2096 T2090 dobj features,affects
R1433 T2702 T2703 compound Snail,mRNA
R1434 T2703 T2704 nsubjpass mRNA,Expressed
R1435 T2705 T2703 cc and,mRNA
R1436 T2706 T2703 conj Protein,mRNA
R1437 T2707 T2704 auxpass Are,Expressed
R1438 T2708 T2704 advmod Transiently,Expressed
R1439 T2709 T2704 prep at,Expressed
R1440 T2710 T2711 det the,Stage
R1441 T2711 T2709 pobj Stage,at
R1442 T2712 T2713 compound Hair,Bud
R1443 T2713 T2711 compound Bud,Stage
R1444 T2714 T2711 prep of,Stage
R1445 T2715 T2716 compound Follicle,Morphogenesis
R1446 T2716 T2714 pobj Morphogenesis,of
R1447 T2718 T2719 mark Although,associated
R1448 T2719 T2726 advcl associated,participate
R1449 T2720 T2721 compound Snail,members
R1450 T2721 T2719 nsubjpass members,associated
R1451 T2722 T2721 compound family,members
R1452 T2723 T2719 auxpass are,associated
R1453 T2724 T2725 advmod most,frequently
R1454 T2725 T2719 advmod frequently,associated
R1455 T2727 T2719 prep with,associated
R1456 T2728 T2727 pobj EMTs,with
R1457 T2729 T2726 punct ", ",participate
R1458 T2730 T2726 nsubj they,participate
R1459 T2731 T2726 advmod also,participate
R1460 T2732 T2726 prep in,participate
R1461 T2733 T2734 amod many,processes
R1462 T2734 T2732 pobj processes,in
R1463 T2735 T2734 amod malignant,processes
R1464 T2736 T2734 acl involving,processes
R1465 T2737 T2738 det a,regulation
R1466 T2738 T2736 dobj regulation,involving
R1467 T2739 T2738 amod down,regulation
R1468 T2740 T2738 punct -,regulation
R1469 T2741 T2738 cc but,regulation
R1470 T2742 T2741 neg not,but
R1471 T2743 T2744 det a,abrogation
R1472 T2744 T2738 conj abrogation,regulation
R1473 T2745 T2744 amod quantitative,abrogation
R1474 T2746 T2738 prep of,regulation
R1475 T2747 T2748 amod intercellular,junctions
R1476 T2748 T2746 pobj junctions,of
R1477 T2749 T2750 punct [,18
R1478 T2750 T2726 parataxis 18,participate
R1479 T2751 T2750 punct ],18
R1480 T2752 T2726 punct .,participate
R1481 T2754 T2755 det The,range
R1482 T2755 T2756 nsubj range,includes
R1483 T2757 T2755 prep of,range
R1484 T2758 T2759 amod developmental,processes
R1485 T2759 T2757 pobj processes,of
R1486 T2760 T2761 prep in,implicated
R1487 T2761 T2759 relcl implicated,processes
R1488 T2762 T2760 pobj which,in
R1489 T2763 T2764 compound Snail,members
R1490 T2764 T2761 nsubjpass members,implicated
R1491 T2765 T2764 compound family,members
R1492 T2766 T2761 aux have,implicated
R1493 T2767 T2761 auxpass been,implicated
R1494 T2768 T2756 advmod thus,includes
R1495 T2769 T2770 det the,type
R1496 T2770 T2756 dobj type,includes
R1497 T2771 T2770 prep of,type
R1498 T2772 T2773 amod epithelial,remodeling
R1499 T2773 T2771 pobj remodeling,of
R1500 T2774 T2775 dep that,observed
R1501 T2775 T2770 relcl observed,type
R1502 T2776 T2775 auxpass is,observed
R1503 T2777 T2775 prep in,observed
R1504 T2778 T2779 compound hair,follicle
R1505 T2779 T2780 compound follicle,bud
R1506 T2780 T2781 compound bud,formation
R1507 T2781 T2777 pobj formation,in
R1508 T2782 T2756 punct .,includes
R1509 T2784 T2785 prep Given,turned
R1510 T2786 T2787 poss our,observation
R1511 T2787 T2784 pobj observation,Given
R1512 T2788 T2787 amod prior,observation
R1513 T2789 T2790 mark that,participate
R1514 T2790 T2787 acl participate,observation
R1515 T2791 T2792 advmod exogenously,added
R1516 T2792 T2793 amod added,Snail
R1517 T2793 T2790 nsubj Snail,participate
R1518 T2794 T2790 aux can,participate
R1519 T2795 T2790 prep with,participate
R1520 T2796 T2795 pobj LEF,with
R1521 T2797 T2796 punct -,LEF
R1522 T2798 T2796 nummod 1,LEF
R1523 T2799 T2796 punct /,LEF
R1524 T2800 T2801 compound β,catenin
R1525 T2801 T2796 appos catenin,LEF
R1526 T2802 T2801 punct -,catenin
R1527 T2803 T2790 prep in,participate
R1528 T2804 T2805 advmod down,regulating
R1529 T2805 T2803 pcomp regulating,in
R1530 T2806 T2805 punct -,regulating
R1531 T2807 T2808 compound E,cadherin
R1532 T2808 T2810 compound cadherin,expression
R1533 T2809 T2808 punct -,cadherin
R1534 T2810 T2805 dobj expression,regulating
R1535 T2811 T2790 prep in,participate
R1536 T2812 T2811 pobj keratinocytes,in
R1537 T2813 T2814 punct [,4
R1538 T2814 T2790 parataxis 4,participate
R1539 T2815 T2814 punct ],4
R1540 T2816 T2787 punct ", ",observation
R1541 T2817 T2787 acl coupled,observation
R1542 T2818 T2817 prep with,coupled
R1543 T2819 T2820 det the,requirement
R1544 T2820 T2818 pobj requirement,with
R1545 T2821 T2820 amod established,requirement
R1546 T2822 T2820 prep for,requirement
R1547 T2823 T2822 pobj LEF,for
R1548 T2824 T2823 punct -,LEF
R1549 T2825 T2823 nummod 1,LEF
R1550 T2826 T2823 punct /,LEF
R1551 T2827 T2828 compound β,catenin
R1552 T2828 T2823 appos catenin,LEF
R1553 T2829 T2828 punct -,catenin
R1554 T2830 T2820 prep in,requirement
R1555 T2831 T2832 compound hair,follicle
R1556 T2832 T2833 compound follicle,morphogenesis
R1557 T2833 T2830 pobj morphogenesis,in
R1558 T2834 T2835 punct [,19
R1559 T2835 T2817 parataxis 19,coupled
R1560 T2836 T2835 nummod 4,19
R1561 T2837 T2835 punct ",",19
R1562 T2838 T2835 punct ],19
R1563 T2839 T2785 punct ", ",turned
R1564 T2840 T2785 nsubj we,turned
R1565 T2841 T2785 prep to,turned
R1566 T2842 T2841 pcomp addressing,to
R1567 T2843 T2844 mark whether,participate
R1568 T2844 T2842 ccomp participate,addressing
R1569 T2845 T2846 compound Snail,Slug
R1570 T2846 T2848 compound Slug,members
R1571 T2847 T2846 punct /,Slug
R1572 T2848 T2844 nsubj members,participate
R1573 T2849 T2848 compound family,members
R1574 T2850 T2844 aux might,participate
R1575 T2851 T2844 advmod also,participate
R1576 T2852 T2844 prep in,participate
R1577 T2853 T2854 det the,process
R1578 T2854 T2852 pobj process,in
R1579 T2855 T2785 punct .,turned
R1580 T2857 T2858 compound PCR,analyses
R1581 T2858 T2859 nsubj analyses,identified
R1582 T2860 T2861 amod transient,expression
R1583 T2861 T2859 dobj expression,identified
R1584 T2862 T2861 compound Snail,expression
R1585 T2863 T2861 compound mRNA,expression
R1586 T2864 T2859 prep during,identified
R1587 T2865 T2866 det a,period
R1588 T2866 T2864 pobj period,during
R1589 T2867 T2866 prep of,period
R1590 T2868 T2869 compound skin,embryogenesis
R1591 T2869 T2867 pobj embryogenesis,of
R1592 T2870 T2871 advmod when,forming
R1593 T2871 T2866 advcl forming,period
R1594 T2872 T2871 nsubj waves,forming
R1595 T2873 T2872 prep of,waves
R1596 T2874 T2875 compound hair,follicles
R1597 T2875 T2873 pobj follicles,of
R1598 T2876 T2871 aux are,forming
R1599 T2877 T2878 punct (,data
R1600 T2878 T2859 meta data,identified
R1601 T2879 T2878 amod unpublished,data
R1602 T2880 T2878 punct ),data
R1603 T2881 T2859 punct .,identified
R1604 T2882 T2883 aux To,pinpoint
R1605 T2883 T2885 advcl pinpoint,conducted
R1606 T2886 T2883 advmod specifically,pinpoint
R1607 T2887 T2888 advmod where,expressed
R1608 T2888 T2883 advcl expressed,pinpoint
R1609 T2889 T2890 compound Snail,mRNA
R1610 T2890 T2888 nsubjpass mRNA,expressed
R1611 T2891 T2888 auxpass is,expressed
R1612 T2892 T2888 prep in,expressed
R1613 T2893 T2894 det the,skin
R1614 T2894 T2892 pobj skin,in
R1615 T2895 T2894 amod developing,skin
R1616 T2896 T2885 punct ", ",conducted
R1617 T2897 T2885 nsubj we,conducted
R1618 T2898 T2899 advmod in,situ
R1619 T2899 T2900 amod situ,hybridization
R1620 T2900 T2885 dobj hybridization,conducted
R1621 T2901 T2885 advcl using,conducted
R1622 T2902 T2903 det a,probe
R1623 T2903 T2901 dobj probe,using
R1624 T2904 T2903 compound cRNA,probe
R1625 T2905 T2903 amod unique,probe
R1626 T2906 T2905 prep to,unique
R1627 T2907 T2908 det the,region
R1628 T2908 T2906 pobj region,to
R1629 T2909 T2908 nmod Snail,region
R1630 T2910 T2908 nummod 3,region
R1631 T2911 T2910 punct ′,3
R1632 T2912 T2908 amod untranslated,region
R1633 T2913 T2908 punct (,region
R1634 T2914 T2908 appos UTR,region
R1635 T2915 T2908 punct ),region
R1636 T2916 T2885 punct .,conducted
R1637 T2918 T2919 amod Embryonic,day
R1638 T2919 T2920 nsubjpass day,chosen
R1639 T2921 T2919 nummod 17.5,day
R1640 T2922 T2919 punct (,day
R1641 T2923 T2919 appos E17.5,day
R1642 T2924 T2920 punct ),chosen
R1643 T2925 T2920 auxpass was,chosen
R1644 T2926 T2920 punct ", ",chosen
R1645 T2927 T2928 mark since,enabled
R1646 T2928 T2920 advcl enabled,chosen
R1647 T2929 T2930 det the,waves
R1648 T2930 T2928 nsubj waves,enabled
R1649 T2931 T2930 amod multiple,waves
R1650 T2932 T2930 prep of,waves
R1651 T2933 T2934 compound follicle,morphogenesis
R1652 T2934 T2932 pobj morphogenesis,of
R1653 T2935 T2930 acl occurring,waves
R1654 T2936 T2935 prep at,occurring
R1655 T2937 T2938 det this,time
R1656 T2938 T2936 pobj time,at
R1657 T2939 T2928 dobj us,enabled
R1658 T2940 T2941 aux to,evaluate
R1659 T2941 T2928 xcomp evaluate,enabled
R1660 T2942 T2943 compound Snail,expression
R1661 T2943 T2941 dobj expression,evaluate
R1662 T2944 T2941 prep at,evaluate
R1663 T2945 T2946 amod different,stages
R1664 T2946 T2944 pobj stages,at
R1665 T2947 T2946 prep of,stages
R1666 T2948 T2949 det the,process
R1667 T2949 T2947 pobj process,of
R1668 T2950 T2920 punct .,chosen
R1669 T2952 T2953 mark As,shown
R1670 T2953 T2954 advcl shown,detected
R1671 T2955 T2953 prep in,shown
R1672 T2956 T2957 compound Figure,1A
R1673 T2957 T2955 pobj 1A,in
R1674 T2958 T2954 punct ", ",detected
R1675 T2959 T2960 amod specific,hybridization
R1676 T2960 T2954 nsubjpass hybridization,detected
R1677 T2961 T2954 auxpass was,detected
R1678 T2962 T2954 prep within,detected
R1679 T2963 T2964 det the,epithelium
R1680 T2964 T2962 pobj epithelium,within
R1681 T2965 T2964 prep of,epithelium
R1682 T2966 T2967 amod nascent,buds
R1683 T2967 T2965 pobj buds,of
R1684 T2968 T2967 compound hair,buds
R1685 T2969 T2954 punct .,detected
R1686 T2971 T2972 prep By,exhibited
R1687 T2973 T2971 pobj contrast,By
R1688 T2974 T2972 punct ", ",exhibited
R1689 T2975 T2976 mark as,progressed
R1690 T2976 T2972 advcl progressed,exhibited
R1691 T2977 T2976 nsubj follicles,progressed
R1692 T2978 T2976 advmod further,progressed
R1693 T2979 T2976 prep through,progressed
R1694 T2980 T2981 poss their,development
R1695 T2981 T2979 pobj development,through
R1696 T2982 T2983 punct (,stages
R1697 T2983 T2976 parataxis stages,progressed
R1698 T2984 T2983 advmod e.g.,stages
R1699 T2985 T2983 punct ", ",stages
R1700 T2986 T2983 nmod germ,stages
R1701 T2987 T2986 cc and,germ
R1702 T2988 T2986 conj peg,germ
R1703 T2989 T2983 punct ),stages
R1704 T2990 T2972 punct ", ",exhibited
R1705 T2991 T2972 nsubj they,exhibited
R1706 T2992 T2993 det no,signs
R1707 T2993 T2972 dobj signs,exhibited
R1708 T2994 T2993 prep of,signs
R1709 T2995 T2994 pobj hybridization,of
R1710 T2996 T2997 punct (,1A
R1711 T2997 T2972 parataxis 1A,exhibited
R1712 T2998 T2997 compound Figure,1A
R1713 T2999 T2997 punct ),1A
R1714 T3000 T2972 punct .,exhibited
R1715 T3002 T3003 det The,nature
R1716 T3003 T3005 nsubj nature,was
R1717 T3004 T3003 amod transient,nature
R1718 T3006 T3003 prep of,nature
R1719 T3007 T3008 compound Snail,expression
R1720 T3008 T3006 pobj expression,of
R1721 T3009 T3008 compound mRNA,expression
R1722 T3010 T3008 prep during,expression
R1723 T3011 T3012 compound follicle,development
R1724 T3012 T3010 pobj development,during
R1725 T3013 T3014 advmod most,apparent
R1726 T3014 T3005 acomp apparent,was
R1727 T3015 T3005 prep in,was
R1728 T3016 T3017 amod hybridized,sections
R1729 T3017 T3015 pobj sections,in
R1730 T3018 T3017 compound skin,sections
R1731 T3019 T3017 acl containing,sections
R1732 T3020 T3019 dobj follicles,containing
R1733 T3021 T3020 prep from,follicles
R1734 T3022 T3023 nummod two,waves
R1735 T3023 T3021 pobj waves,from
R1736 T3024 T3023 amod different,waves
R1737 T3025 T3023 prep of,waves
R1738 T3026 T3025 pobj morphogenesis,of
R1739 T3027 T3028 punct (,shown
R1740 T3028 T3005 parataxis shown,was
R1741 T3029 T3028 mark as,shown
R1742 T3030 T3028 prep in,shown
R1743 T3031 T3030 pobj Figure,in
R1744 T3032 T3031 nummod 1,Figure
R1745 T3033 T3028 punct ),shown
R1746 T3034 T3005 punct .,was
R1747 T3036 T3037 amod Hybridizing,buds
R1748 T3037 T3039 nsubj buds,appeared
R1749 T3038 T3037 compound hair,buds
R1750 T3040 T3037 prep from,buds
R1751 T3041 T3042 det a,wave
R1752 T3042 T3040 pobj wave,from
R1753 T3043 T3042 amod later,wave
R1754 T3044 T3039 oprd juxtaposed,appeared
R1755 T3045 T3044 prep with,juxtaposed
R1756 T3046 T3047 amod nonhybridizing,follicles
R1757 T3047 T3045 pobj follicles,with
R1758 T3048 T3047 prep from,follicles
R1759 T3049 T3050 det an,wave
R1760 T3050 T3048 pobj wave,from
R1761 T3051 T3050 amod earlier,wave
R1762 T3052 T3039 punct .,appeared
R1763 T3054 T3055 aux To,determine
R1764 T3055 T3056 advcl determine,generated
R1765 T3057 T3058 mark whether,reflected
R1766 T3058 T3055 ccomp reflected,determine
R1767 T3059 T3060 det this,nature
R1768 T3060 T3058 nsubjpass nature,reflected
R1769 T3061 T3060 amod transient,nature
R1770 T3062 T3060 prep of,nature
R1771 T3063 T3064 compound Snail,expression
R1772 T3064 T3062 pobj expression,of
R1773 T3065 T3064 compound mRNA,expression
R1774 T3066 T3058 auxpass is,reflected
R1775 T3067 T3058 prep at,reflected
R1776 T3068 T3069 det the,level
R1777 T3069 T3067 pobj level,at
R1778 T3070 T3069 compound protein,level
R1779 T3071 T3056 punct ", ",generated
R1780 T3072 T3056 nsubj we,generated
R1781 T3073 T3074 det an,antibody
R1782 T3074 T3056 dobj antibody,generated
R1783 T3075 T3074 prep against,antibody
R1784 T3076 T3077 det the,sequence
R1785 T3077 T3075 pobj sequence,against
R1786 T3078 T3079 npadvmod N,terminal
R1787 T3079 T3077 amod terminal,sequence
R1788 T3080 T3079 punct -,terminal
R1789 T3081 T3082 dep that,resides
R1790 T3082 T3077 relcl resides,sequence
R1791 T3083 T3082 advmod upstream,resides
R1792 T3084 T3083 prep of,upstream
R1793 T3085 T3086 det the,domains
R1794 T3086 T3084 pobj domains,of
R1795 T3087 T3088 advmod more,conserved
R1796 T3088 T3086 amod conserved,domains
R1797 T3089 T3090 compound zinc,finger
R1798 T3090 T3086 compound finger,domains
R1799 T3091 T3056 punct .,generated
R1800 T3093 T3094 mark As,judged
R1801 T3094 T3095 advcl judged,detect
R1802 T3096 T3094 prep by,judged
R1803 T3097 T3098 compound Western,blot
R1804 T3098 T3099 compound blot,analysis
R1805 T3099 T3096 pobj analysis,by
R1806 T3100 T3095 punct ", ",detect
R1807 T3101 T3102 det the,antibody
R1808 T3102 T3095 nsubj antibody,detect
R1809 T3103 T3095 aux did,detect
R1810 T3104 T3095 neg not,detect
R1811 T3105 T3106 amod endogenous,proteins
R1812 T3106 T3095 dobj proteins,detect
R1813 T3107 T3106 prep from,proteins
R1814 T3108 T3109 amod cultured,keratinocytes
R1815 T3109 T3107 pobj keratinocytes,from
R1816 T3110 T3095 punct ", ",detect
R1817 T3111 T3095 cc but,detect
R1818 T3112 T3113 nsubj it,yield
R1819 T3113 T3095 conj yield,detect
R1820 T3114 T3113 aux did,yield
R1821 T3115 T3116 det a,band
R1822 T3116 T3113 dobj band,yield
R1823 T3117 T3116 prep of,band
R1824 T3118 T3119 det the,size
R1825 T3119 T3117 pobj size,of
R1826 T3120 T3119 amod expected,size
R1827 T3121 T3113 prep from,yield
R1828 T3122 T3121 pobj keratinocytes,from
R1829 T3123 T3124 advmod transiently,expressing
R1830 T3124 T3122 acl expressing,keratinocytes
R1831 T3125 T3126 det a,protein
R1832 T3126 T3124 dobj protein,expressing
R1833 T3127 T3128 npadvmod hemagglutinin,tagged
R1834 T3128 T3126 amod tagged,protein
R1835 T3129 T3127 punct (,hemagglutinin
R1836 T3130 T3127 appos HA,hemagglutinin
R1837 T3131 T3128 punct ),tagged
R1838 T3132 T3128 punct -,tagged
R1839 T3133 T3126 compound Snail,protein
R1840 T3134 T3135 punct (,1B
R1841 T3135 T3113 parataxis 1B,yield
R1842 T3136 T3135 compound Figure,1B
R1843 T3137 T3135 punct ),1B
R1844 T3138 T3095 punct .,detect
R1845 T3140 T3141 det The,antibody
R1846 T3141 T3142 nsubj antibody,recognized
R1847 T3143 T3142 advmod also,recognized
R1848 T3144 T3145 det a,band
R1849 T3145 T3142 dobj band,recognized
R1850 T3146 T3145 acl corresponding,band
R1851 T3147 T3146 prep to,corresponding
R1852 T3148 T3149 det the,size
R1853 T3149 T3147 pobj size,to
R1854 T3150 T3149 prep of,size
R1855 T3151 T3152 amod endogenous,Snail
R1856 T3152 T3150 pobj Snail,of
R1857 T3153 T3149 punct (,size
R1858 T3154 T3155 advmod approximately,28
R1859 T3155 T3156 nummod 28,kDa
R1860 T3156 T3149 appos kDa,size
R1861 T3157 T3149 punct ),size
R1862 T3158 T3149 prep in,size
R1863 T3159 T3158 pobj lysates,in
R1864 T3160 T3159 prep from,lysates
R1865 T3161 T3162 amod embryonic,skin
R1866 T3162 T3160 pobj skin,from
R1867 T3163 T3162 compound mouse,skin
R1868 T3164 T3145 punct ", ",band
R1869 T3165 T3166 det the,appearance
R1870 T3166 T3168 dep appearance,corresponded
R1871 T3167 T3166 amod temporal,appearance
R1872 T3168 T3145 relcl corresponded,band
R1873 T3169 T3166 prep of,appearance
R1874 T3170 T3169 pobj which,of
R1875 T3171 T3168 prep to,corresponded
R1876 T3172 T3173 det the,waves
R1877 T3173 T3171 pobj waves,to
R1878 T3174 T3173 prep of,waves
R1879 T3175 T3176 compound hair,follicle
R1880 T3176 T3177 compound follicle,morphogenesis
R1881 T3177 T3174 pobj morphogenesis,of
R1882 T3178 T3173 prep from,waves
R1883 T3179 T3178 pobj E15.5,from
R1884 T3180 T3178 prep to,from
R1885 T3181 T3180 amod newborn,to
R1886 T3182 T3183 advmod when,formed
R1887 T3183 T3173 relcl formed,waves
R1888 T3184 T3185 quantmod over,90
R1889 T3185 T3186 nummod 90,%
R1890 T3186 T3183 nsubjpass %,formed
R1891 T3187 T3186 prep of,%
R1892 T3188 T3189 det the,hair
R1893 T3189 T3187 pobj hair,of
R1894 T3190 T3189 prep on,hair
R1895 T3191 T3192 det the,mouse
R1896 T3192 T3190 pobj mouse,on
R1897 T3193 T3183 auxpass is,formed
R1898 T3194 T3195 punct (,1B
R1899 T3195 T3142 parataxis 1B,recognized
R1900 T3196 T3195 compound Figure,1B
R1901 T3197 T3195 punct ),1B
R1902 T3198 T3142 punct .,recognized
R1903 T3200 T3201 advcl Consistent,detect
R1904 T3202 T3200 prep with,Consistent
R1905 T3203 T3204 det the,data
R1906 T3204 T3202 pobj data,with
R1907 T3205 T3206 compound Western,blot
R1908 T3206 T3204 compound blot,data
R1909 T3207 T3201 punct ", ",detect
R1910 T3208 T3209 amod immunohistochemical,analysis
R1911 T3209 T3201 nsubj analysis,detect
R1912 T3210 T3201 aux did,detect
R1913 T3211 T3201 neg not,detect
R1914 T3212 T3201 dobj Snail,detect
R1915 T3213 T3201 prep in,detect
R1916 T3214 T3215 amod single,layered
R1917 T3215 T3217 amod layered,epidermis
R1918 T3216 T3215 punct -,layered
R1919 T3217 T3213 pobj epidermis,in
R1920 T3218 T3217 compound E13.5,epidermis
R1921 T3219 T3220 punct (,1C
R1922 T3220 T3217 parataxis 1C,epidermis
R1923 T3221 T3220 compound Figure,1C
R1924 T3222 T3220 punct ),1C
R1925 T3223 T3213 cc nor,in
R1926 T3224 T3213 conj in,in
R1927 T3225 T3226 det the,placode
R1928 T3226 T3224 pobj placode,in
R1929 T3227 T3201 punct ", ",detect
R1930 T3228 T3229 dep which,is
R1931 T3229 T3201 advcl is,detect
R1932 T3230 T3231 det the,sign
R1933 T3231 T3229 attr sign,is
R1934 T3232 T3231 amod earliest,sign
R1935 T3233 T3231 amod morphological,sign
R1936 T3234 T3231 prep of,sign
R1937 T3235 T3236 det the,commitment
R1938 T3236 T3234 pobj commitment,of
R1939 T3237 T3236 prep of,commitment
R1940 T3238 T3239 amod multipotent,cells
R1941 T3239 T3237 pobj cells,of
R1942 T3240 T3239 prep of,cells
R1943 T3241 T3242 det the,ectoderm
R1944 T3242 T3240 pobj ectoderm,of
R1945 T3243 T3242 amod embryonic,ectoderm
R1946 T3244 T3236 prep to,commitment
R1947 T3245 T3246 det a,fate
R1948 T3246 T3244 pobj fate,to
R1949 T3247 T3248 compound hair,cell
R1950 T3248 T3246 compound cell,fate
R1951 T3249 T3250 punct (,1D
R1952 T3250 T3201 parataxis 1D,detect
R1953 T3251 T3250 compound Figure,1D
R1954 T3252 T3250 punct ),1D
R1955 T3253 T3201 punct .,detect
R1956 T3255 T3256 advcl Consistent,labeled
R1957 T3257 T3255 prep with,Consistent
R1958 T3258 T3259 det the,results
R1959 T3259 T3257 pobj results,with
R1960 T3260 T3261 advmod in,situ
R1961 T3261 T3259 amod situ,results
R1962 T3262 T3259 compound hybridization,results
R1963 T3263 T3256 punct ", ",labeled
R1964 T3264 T3265 amod anti-Snail,antibody
R1965 T3265 T3256 nsubj antibody,labeled
R1966 T3266 T3267 advmod only,buds
R1967 T3267 T3256 dobj buds,labeled
R1968 T3268 T3267 compound hair,buds
R1969 T3269 T3267 cc and,buds
R1970 T3270 T3269 neg not,and
R1971 T3271 T3267 conj follicles,buds
R1972 T3272 T3256 prep at,labeled
R1973 T3273 T3274 advmod more,stages
R1974 T3274 T3272 pobj stages,at
R1975 T3275 T3274 amod mature,stages
R1976 T3276 T3274 prep of,stages
R1977 T3277 T3276 pobj development,of
R1978 T3278 T3279 punct (,1E
R1979 T3279 T3256 parataxis 1E,labeled
R1980 T3280 T3279 compound Figure,1E
R1981 T3281 T3279 punct ),1E
R1982 T3282 T3256 punct .,labeled
R1983 T3284 T3285 advcl Taken,appeared
R1984 T3286 T3284 advmod together,Taken
R1985 T3287 T3285 punct ", ",appeared
R1986 T3288 T3289 det the,antibody
R1987 T3289 T3285 nsubj antibody,appeared
R1988 T3290 T3289 amod anti-Snail,antibody
R1989 T3291 T3292 aux to,be
R1990 T3292 T3285 xcomp be,appeared
R1991 T3293 T3292 acomp specific,be
R1992 T3294 T3293 prep for,specific
R1993 T3295 T3296 poss its,protein
R1994 T3296 T3294 pobj protein,for
R1995 T3297 T3296 compound target,protein
R1996 T3298 T3285 punct .,appeared
R1997 T3300 T3301 nsubj It,detect
R1998 T3302 T3301 aux did,detect
R1999 T3303 T3301 neg not,detect
R2000 T3304 T3305 amod other,members
R2001 T3305 T3301 dobj members,detect
R2002 T3306 T3305 compound Snail,members
R2003 T3307 T3305 compound family,members
R2004 T3308 T3305 acl known,members
R2005 T3309 T3310 aux to,expressed
R2006 T3310 T3308 xcomp expressed,known
R2007 T3311 T3310 auxpass be,expressed
R2008 T3312 T3310 prep in,expressed
R2009 T3313 T3312 pobj keratinocytes,in
R2010 T3314 T3313 cc and,keratinocytes
R2011 T3315 T3314 punct /,and
R2012 T3316 T3314 cc or,and
R2013 T3317 T3313 conj skin,keratinocytes
R2014 T3318 T3319 punct (,data
R2015 T3319 T3308 meta data,known
R2016 T3320 T3319 amod unpublished,data
R2017 T3321 T3319 punct ),data
R2018 T3322 T3301 punct .,detect
R2019 T3324 T3325 advmod Furthermore,paralleled
R2020 T3326 T3325 punct ", ",paralleled
R2021 T3327 T3328 det the,data
R2022 T3328 T3325 nsubj data,paralleled
R2023 T3329 T3328 amod immunohistochemical,data
R2024 T3330 T3331 poss our,data
R2025 T3331 T3325 dobj data,paralleled
R2026 T3332 T3333 nmod Snail,hybridization
R2027 T3333 T3331 compound hybridization,data
R2028 T3334 T3335 advmod in,situ
R2029 T3335 T3333 amod situ,hybridization
R2030 T3336 T3325 advcl revealing,paralleled
R2031 T3337 T3338 amod transient,expression
R2032 T3338 T3336 dobj expression,revealing
R2033 T3339 T3338 compound Snail,expression
R2034 T3340 T3336 prep at,revealing
R2035 T3341 T3342 det the,stage
R2036 T3342 T3340 pobj stage,at
R2037 T3343 T3344 compound hair,bud
R2038 T3344 T3342 compound bud,stage
R2039 T3345 T3346 punct (,1A
R2040 T3346 T3325 parataxis 1A,paralleled
R2041 T3347 T3346 compound Figure,1A
R2042 T3348 T3346 punct ),1A
R2043 T3349 T3325 punct .,paralleled
R2044 T3351 T3352 mark As,judged
R2045 T3352 T3353 advcl judged,localized
R2046 T3354 T3352 prep by,judged
R2047 T3355 T3354 pobj immunohistochemistry,by
R2048 T3356 T3353 punct ", ",localized
R2049 T3357 T3358 compound Snail,protein
R2050 T3358 T3353 nsubjpass protein,localized
R2051 T3359 T3353 auxpass was,localized
R2052 T3360 T3353 prep to,localized
R2053 T3361 T3362 det the,nuclei
R2054 T3362 T3360 pobj nuclei,to
R2055 T3363 T3362 prep of,nuclei
R2056 T3364 T3365 det the,cells
R2057 T3365 T3363 pobj cells,of
R2058 T3366 T3367 compound hair,bud
R2059 T3367 T3365 compound bud,cells
R2060 T3368 T3369 punct (,1E
R2061 T3369 T3353 parataxis 1E,localized
R2062 T3370 T3369 compound Figure,1E
R2063 T3371 T3369 punct ),1E
R2064 T3372 T3353 punct .,localized
R2065 T3374 T3375 det This,feature
R2066 T3375 T3376 nsubj feature,was
R2067 T3377 T3376 acomp consistent,was
R2068 T3378 T3377 prep with,consistent
R2069 T3379 T3380 poss Snail,function
R2070 T3380 T3378 pobj function,with
R2071 T3381 T3379 case 's,Snail
R2072 T3382 T3380 amod known,function
R2073 T3383 T3380 prep as,function
R2074 T3384 T3385 det a,repressor
R2075 T3385 T3383 pobj repressor,as
R2076 T3386 T3385 amod transcriptional,repressor
R2077 T3387 T3388 punct [,13
R2078 T3388 T3376 parataxis 13,was
R2079 T3389 T3388 nummod 12,13
R2080 T3390 T3388 punct ",",13
R2081 T3391 T3388 punct ],13
R2082 T3392 T3376 punct .,was
R2083 T3394 T3395 advmod Additionally,detected
R2084 T3396 T3395 punct ", ",detected
R2085 T3397 T3398 amod anti-Snail,labeling
R2086 T3398 T3395 nsubjpass labeling,detected
R2087 T3399 T3395 auxpass was,detected
R2088 T3400 T3395 prep in,detected
R2089 T3401 T3402 advmod only,four
R2090 T3402 T3406 nummod four,waves
R2091 T3403 T3402 quantmod three,four
R2092 T3404 T3402 quantmod of,four
R2093 T3405 T3402 quantmod the,four
R2094 T3406 T3400 pobj waves,in
R2095 T3407 T3406 amod major,waves
R2096 T3408 T3406 prep of,waves
R2097 T3409 T3410 compound follicle,morphogenesis
R2098 T3410 T3408 pobj morphogenesis,of
R2099 T3411 T3395 punct .,detected
R2100 T3413 T3414 nsubjpass Snail,found
R2101 T3415 T3414 auxpass was,found
R2102 T3416 T3414 neg not,found
R2103 T3417 T3414 prep in,found
R2104 T3418 T3419 det the,buds
R2105 T3419 T3417 pobj buds,in
R2106 T3420 T3419 prep of,buds
R2107 T3421 T3422 compound guard,hairs
R2108 T3422 T3420 pobj hairs,of
R2109 T3423 T3424 dep that,are
R2110 T3424 T3422 advcl are,hairs
R2111 T3425 T3426 det the,earliest
R2112 T3426 T3424 attr earliest,are
R2113 T3427 T3426 prep of,earliest
R2114 T3428 T3429 det all,hairs
R2115 T3429 T3427 pobj hairs,of
R2116 T3430 T3431 aux to,form
R2117 T3431 T3429 advcl form,hairs
R2118 T3432 T3433 punct (,at
R2119 T3433 T3426 parataxis at,earliest
R2120 T3434 T3433 pobj E13.5,at
R2121 T3435 T3433 punct ),at
R2122 T3436 T3424 punct ", ",are
R2123 T3437 T3424 cc and,are
R2124 T3438 T3439 dep which,constitute
R2125 T3439 T3424 conj constitute,are
R2126 T3440 T3441 amod less,5
R2127 T3441 T3443 nummod 5,%
R2128 T3442 T3441 quantmod than,5
R2129 T3443 T3439 dobj %,constitute
R2130 T3444 T3443 prep of,%
R2131 T3445 T3446 det the,coat
R2132 T3446 T3444 pobj coat,of
R2133 T3447 T3446 compound mouse,coat
R2134 T3448 T3449 punct (,data
R2135 T3449 T3414 meta data,found
R2136 T3450 T3449 amod unpublished,data
R2137 T3451 T3449 punct ),data
R2138 T3452 T3414 punct .,found
R2139 T3454 T3455 mark As,judged
R2140 T3455 T3456 advcl judged,coincided
R2141 T3457 T3455 prep by,judged
R2142 T3458 T3457 pobj immunofluorescence,by
R2143 T3459 T3458 prep with,immunofluorescence
R2144 T3460 T3459 pobj antibodies,with
R2145 T3461 T3460 prep against,antibodies
R2146 T3462 T3463 det the,antigen
R2147 T3463 T3461 pobj antigen,against
R2148 T3464 T3463 amod proliferating,antigen
R2149 T3465 T3463 amod nuclear,antigen
R2150 T3466 T3463 appos Ki67,antigen
R2151 T3467 T3456 punct ", ",coincided
R2152 T3468 T3469 det the,timing
R2153 T3469 T3456 nsubj timing,coincided
R2154 T3470 T3469 prep of,timing
R2155 T3471 T3472 compound Snail,expression
R2156 T3472 T3470 pobj expression,of
R2157 T3473 T3456 prep with,coincided
R2158 T3474 T3475 det the,stage
R2159 T3475 T3473 pobj stage,with
R2160 T3476 T3477 prep at,enhanced
R2161 T3477 T3475 relcl enhanced,stage
R2162 T3478 T3476 pobj which,at
R2163 T3479 T3480 det the,follicle
R2164 T3480 T3477 nsubj follicle,enhanced
R2165 T3481 T3480 amod developing,follicle
R2166 T3482 T3483 poss its,proliferation
R2167 T3483 T3477 dobj proliferation,enhanced
R2168 T3484 T3483 cc and,proliferation
R2169 T3485 T3486 amod down,growth
R2170 T3486 T3483 conj growth,proliferation
R2171 T3487 T3486 punct -,growth
R2172 T3488 T3489 punct (,1F
R2173 T3489 T3456 parataxis 1F,coincided
R2174 T3490 T3489 compound Figure,1F
R2175 T3491 T3489 punct ),1F
R2176 T3492 T3456 punct .,coincided
R2177 T3494 T3495 nsubj Immunohistochemistry,marked
R2178 T3496 T3494 prep with,Immunohistochemistry
R2179 T3497 T3496 pobj antibodies,with
R2180 T3498 T3497 prep against,antibodies
R2181 T3499 T3500 det the,form
R2182 T3500 T3498 pobj form,against
R2183 T3501 T3500 amod active,form
R2184 T3502 T3500 punct (,form
R2185 T3503 T3500 amod phosphorylated,form
R2186 T3504 T3500 punct ),form
R2187 T3505 T3500 prep of,form
R2188 T3506 T3505 pobj MAPK,of
R2189 T3507 T3500 punct (,form
R2190 T3508 T3500 appos pMAPK,form
R2191 T3509 T3495 punct ),marked
R2192 T3510 T3511 det a,subset
R2193 T3511 T3495 dobj subset,marked
R2194 T3512 T3511 prep of,subset
R2195 T3513 T3514 det the,cells
R2196 T3514 T3512 pobj cells,of
R2197 T3601 T3599 punct ;,1H
R2198 T3515 T3514 amod proliferating,cells
R2199 T3602 T3599 advcl see,1H
R2200 T3516 T3514 punct (,cells
R2201 T3603 T3602 advmod also,see
R2202 T3604 T3602 punct [,see
R2203 T3517 T3518 npadvmod Ki67,positive
R2204 T3605 T3602 dobj 4,see
R2205 T3606 T3599 punct ],1H
R2206 T3607 T3599 punct ),1H
R2207 T3518 T3514 amod positive,cells
R2208 T3608 T3583 punct .,exhibited
R2209 T3519 T3518 punct -,positive
R2210 T3520 T3514 punct ),cells
R2211 T3521 T3495 punct ", ",marked
R2212 T3522 T3495 cc and,marked
R2213 T3523 T3524 npadvmod pMAPK,positive
R2214 T3524 T3526 amod positive,cells
R2215 T3525 T3524 punct -,positive
R2216 T3526 T3527 nsubjpass cells,enriched
R2217 T3527 T3495 conj enriched,marked
R2218 T3528 T3527 auxpass were,enriched
R2219 T3529 T3527 prep in,enriched
R2220 T3530 T3531 det the,bud
R2221 T3531 T3529 pobj bud,in
R2222 T3532 T3531 compound hair,bud
R2223 T3533 T3534 punct (,1G
R2224 T3534 T3527 parataxis 1G,enriched
R2225 T3535 T3534 compound Figure,1G
R2226 T3536 T3534 punct ),1G
R2227 T3537 T3527 punct .,enriched
R2228 T3539 T3540 det The,timing
R2229 T3540 T3541 nsubj timing,appeared
R2230 T3542 T3540 prep of,timing
R2231 T3543 T3544 compound Snail,induction
R2232 T3544 T3542 pobj induction,of
R2233 T3545 T3544 cc and,induction
R2234 T3546 T3547 nmod Ki67,enrichment
R2235 T3547 T3544 conj enrichment,induction
R2236 T3548 T3546 cc and,Ki67
R2237 T3549 T3546 conj pMAPK,Ki67
R2238 T3550 T3540 prep in,timing
R2239 T3551 T3552 det the,bud
R2240 T3552 T3550 pobj bud,in
R2241 T3553 T3552 compound hair,bud
R2242 T3554 T3555 aux to,follow
R2243 T3555 T3541 xcomp follow,appeared
R2244 T3556 T3555 advmod closely,follow
R2245 T3557 T3558 det the,induction
R2246 T3558 T3555 dobj induction,follow
R2247 T3559 T3558 prep of,induction
R2248 T3560 T3561 nmod LEF,activity
R2249 T3561 T3559 pobj activity,of
R2250 T3562 T3560 punct -,LEF
R2251 T3563 T3560 nummod 1,LEF
R2252 T3564 T3560 punct /,LEF
R2253 T3565 T3566 compound β,catenin
R2254 T3566 T3560 appos catenin,LEF
R2255 T3567 T3566 punct -,catenin
R2256 T3568 T3561 punct ", ",activity
R2257 T3569 T3561 acl known,activity
R2258 T3570 T3571 aux to,initiate
R2259 T3571 T3569 xcomp initiate,known
R2260 T3572 T3571 prep in,initiate
R2261 T3573 T3574 det the,stage
R2262 T3574 T3572 pobj stage,in
R2263 T3575 T3576 compound hair,placode
R2264 T3576 T3574 compound placode,stage
R2265 T3577 T3578 punct [,20
R2266 T3578 T3555 parataxis 20,follow
R2267 T3579 T3578 punct ],20
R2268 T3580 T3541 punct .,appeared
R2269 T3582 T3583 advmod However,exhibited
R2270 T3584 T3583 punct ", ",exhibited
R2271 T3585 T3583 prep like,exhibited
R2272 T3586 T3585 pobj placodes,like
R2273 T3587 T3583 punct ", ",exhibited
R2274 T3588 T3589 compound hair,buds
R2275 T3589 T3583 nsubj buds,exhibited
R2276 T3590 T3591 amod down,regulation
R2277 T3591 T3583 dobj regulation,exhibited
R2278 T3592 T3591 punct -,regulation
R2279 T3593 T3591 prep in,regulation
R2280 T3594 T3595 compound E,cadherin
R2281 T3595 T3597 compound cadherin,expression
R2282 T3596 T3595 punct -,cadherin
R2283 T3597 T3593 pobj expression,in
R2284 T3598 T3599 punct (,1H
R2285 T3599 T3583 parataxis 1H,exhibited
R2286 T3600 T3599 compound Figure,1H
R2289 T3910 T3911 amod Sustained,Expression
R2290 T3911 T3912 nsubj Expression,Results
R2291 T3913 T3911 prep of,Expression
R2292 T3914 T3913 pobj Snail,of
R2293 T3915 T3912 prep in,Results
R2294 T3916 T3917 amod Epidermal,Hyperproliferation
R2295 T3917 T3915 pobj Hyperproliferation,in
R2296 T3918 T3917 cc and,Hyperproliferation
R2297 T3919 T3920 compound Differentiation,Defects
R2298 T3920 T3917 conj Defects,Hyperproliferation
R2299 T3921 T3912 prep in,Results
R2300 T3922 T3923 compound Tg,Skin
R2301 T3923 T3921 pobj Skin,in
R2302 T3924 T3923 compound Mouse,Skin
R2303 T3926 T3927 det The,spike
R2304 T3927 T3929 nsubj spike,prompted
R2305 T3928 T3927 amod striking,spike
R2306 T3930 T3927 prep of,spike
R2307 T3931 T3932 compound Snail,expression
R2308 T3932 T3930 pobj expression,of
R2309 T3933 T3927 amod coincident,spike
R2310 T3934 T3933 prep with,coincident
R2311 T3935 T3936 compound hair,bud
R2312 T3936 T3937 compound bud,formation
R2313 T3937 T3934 pobj formation,with
R2314 T3938 T3937 cc and,formation
R2315 T3939 T3940 amod enhanced,proliferation
R2316 T3940 T3937 conj proliferation,formation
R2317 T3941 T3929 dobj us,prompted
R2318 T3942 T3943 aux to,examine
R2319 T3943 T3929 xcomp examine,prompted
R2320 T3944 T3945 det the,consequences
R2321 T3945 T3943 dobj consequences,examine
R2322 T3946 T3945 prep of,consequences
R2323 T3947 T3948 advmod ectopically,expressing
R2324 T3948 T3946 pcomp expressing,of
R2325 T3949 T3948 dobj Snail,expressing
R2326 T3950 T3948 advmod elsewhere,expressing
R2327 T3951 T3950 prep in,elsewhere
R2328 T3952 T3953 compound mouse,epidermis
R2329 T3953 T3951 pobj epidermis,in
R2330 T3954 T3953 compound skin,epidermis
R2331 T3955 T3929 punct .,prompted
R2332 T3957 T3958 aux To,distinguish
R2333 T3958 T3959 advcl distinguish,used
R2334 T3960 T3958 dobj Tg,distinguish
R2335 T3961 T3958 prep from,distinguish
R2336 T3962 T3963 amod endogenous,Snail
R2337 T3963 T3961 pobj Snail,from
R2338 T3964 T3959 punct ", ",used
R2339 T3965 T3959 nsubj we,used
R2340 T3966 T3967 det the,epitope
R2341 T3967 T3959 dobj epitope,used
R2342 T3968 T3967 compound HA,epitope
R2343 T3969 T3967 punct -,epitope
R2344 T3970 T3967 punct ", ",epitope
R2345 T3971 T3967 acl shown,epitope
R2346 T3972 T3971 advmod previously,shown
R2347 T3973 T3974 neg not,alter
R2348 T3974 T3971 xcomp alter,shown
R2349 T3975 T3974 aux to,alter
R2350 T3976 T3977 poss Snail,activity
R2351 T3977 T3974 dobj activity,alter
R2352 T3978 T3976 case 's,Snail
R2353 T3979 T3977 amod transcriptional,activity
R2354 T3980 T3981 punct [,12
R2355 T3981 T3959 parataxis 12,used
R2356 T3982 T3981 punct ],12
R2357 T3983 T3959 punct .,used
R2358 T3985 T3986 prep Of,expressed
R2359 T3987 T3988 nummod 20,animals
R2360 T3988 T3985 pobj animals,Of
R2361 T3989 T3990 nmod K14,Snail
R2362 T3990 T3988 nmod Snail,animals
R2363 T3991 T3990 punct -,Snail
R2364 T3992 T3988 punct [,animals
R2365 T3993 T3988 nmod HA,animals
R2366 T3994 T3988 punct ],animals
R2367 T3995 T3988 compound Tg,animals
R2368 T3996 T3988 acl generated,animals
R2369 T3997 T3986 punct ", ",expressed
R2370 T3998 T3986 nsubj three,expressed
R2371 T3999 T4000 det the,transgene
R2372 T4000 T3986 dobj transgene,expressed
R2373 T4001 T3986 cc and,expressed
R2374 T4002 T4003 nsubj all,exhibited
R2375 T4003 T3986 conj exhibited,expressed
R2376 T4004 T4005 amod analogous,phenotypes
R2377 T4005 T4003 dobj phenotypes,exhibited
R2378 T4006 T3986 punct .,expressed
R2379 T4008 T4009 nsubj Mice,died
R2380 T4010 T4011 dep that,integrated
R2381 T4011 T4008 relcl integrated,Mice
R2382 T4012 T4013 det the,transgene
R2383 T4013 T4011 dobj transgene,integrated
R2384 T4014 T4011 prep at,integrated
R2385 T4015 T4016 det the,stage
R2386 T4016 T4014 pobj stage,at
R2387 T4017 T4018 amod single,cell
R2388 T4018 T4016 compound cell,stage
R2389 T4019 T4018 punct -,cell
R2390 T4020 T4009 prep at,died
R2391 T4021 T4020 cc or,at
R2392 T4022 T4023 advmod shortly,after
R2393 T4023 T4020 conj after,at
R2394 T4024 T4023 pobj birth,after
R2395 T4025 T4009 punct .,died
R2396 T4027 T4028 det The,mice
R2397 T4028 T4035 nsubj mice,harbored
R2398 T4029 T4028 nummod three,mice
R2399 T4030 T4028 amod surviving,mice
R2400 T4031 T4032 amod full,Tg
R2401 T4032 T4028 compound Tg,mice
R2402 T4033 T4032 punct -,Tg
R2403 T4034 T4028 compound founder,mice
R2404 T4036 T4037 compound transgene,integrations
R2405 T4037 T4035 dobj integrations,harbored
R2406 T4038 T4039 dep that,gave
R2407 T4039 T4037 relcl gave,integrations
R2408 T4040 T4041 amod stable,transmission
R2409 T4041 T4039 dobj transmission,gave
R2410 T4042 T4041 prep of,transmission
R2411 T4043 T4044 amod mosaic,gene
R2412 T4044 T4046 compound gene,expression
R2413 T4045 T4044 compound Snail,gene
R2414 T4046 T4042 pobj expression,of
R2415 T4047 T4039 prep through,gave
R2416 T4048 T4049 det the,germline
R2417 T4049 T4047 pobj germline,through
R2418 T4050 T4035 punct .,harbored
R2419 T4052 T4053 advmod Progressively,poor
R2420 T4053 T4054 amod poor,health
R2421 T4054 T4055 nsubj health,necessitated
R2422 T4056 T4057 nsubj our,sacrificing
R2423 T4057 T4055 ccomp sacrificing,necessitated
R2424 T4058 T4059 amod most,offspring
R2425 T4059 T4057 dobj offspring,sacrificing
R2426 T4060 T4059 prep from,offspring
R2427 T4061 T4062 det these,lines
R2428 T4062 T4060 pobj lines,from
R2429 T4063 T4057 prep within,sacrificing
R2430 T4064 T4065 det a,year
R2431 T4065 T4063 pobj year,within
R2432 T4066 T4065 prep of,year
R2433 T4067 T4066 pobj birth,of
R2434 T4068 T4055 punct .,necessitated
R2435 T4070 T4071 mark As,grew
R2436 T4071 T4075 advcl grew,became
R2437 T4072 T4073 compound Snail,animals
R2438 T4073 T4071 nsubj animals,grew
R2439 T4074 T4073 compound Tg,animals
R2440 T4076 T4075 punct ", ",became
R2441 T4077 T4075 nsubj they,became
R2442 T4078 T4075 acomp distinguished,became
R2443 T4079 T4078 prep by,distinguished
R2444 T4080 T4081 poss their,size
R2445 T4081 T4079 pobj size,by
R2446 T4082 T4081 amod small,size
R2447 T4083 T4081 punct ", ",size
R2448 T4084 T4085 amod short,tails
R2449 T4085 T4081 conj tails,size
R2450 T4086 T4085 punct ", ",tails
R2451 T4087 T4085 cc and,tails
R2452 T4088 T4089 amod flaky,skin
R2453 T4089 T4085 conj skin,tails
R2454 T4090 T4091 punct (,2A
R2455 T4091 T4075 parataxis 2A,became
R2456 T4092 T4091 compound Figure,2A
R2457 T4093 T4091 punct ),2A
R2458 T4094 T4075 punct .,became
R2459 T4096 T4097 amod Histological,analyses
R2460 T4097 T4098 nsubj analyses,revealed
R2461 T4099 T4097 prep of,analyses
R2462 T4100 T4101 nummod 3,d
R2463 T4101 T4103 npadvmod d,old
R2464 T4102 T4101 punct -,d
R2465 T4103 T4104 amod old,mice
R2466 T4104 T4099 pobj mice,of
R2467 T4105 T4106 punct (,P3
R2468 T4106 T4103 parataxis P3,old
R2469 T4107 T4106 punct ),P3
R2470 T4108 T4109 compound mosaic,patches
R2471 T4109 T4098 dobj patches,revealed
R2472 T4110 T4109 acl marked,patches
R2473 T4111 T4110 agent by,marked
R2474 T4112 T4113 amod epidermal,thickening
R2475 T4113 T4111 pobj thickening,by
R2476 T4114 T4115 punct (,2B
R2477 T4115 T4098 parataxis 2B,revealed
R2478 T4116 T4115 compound Figure,2B
R2479 T4117 T4115 punct ),2B
R2480 T4118 T4098 punct .,revealed
R2481 T4120 T4121 det The,morphology
R2482 T4121 T4123 nsubjpass morphology,reflected
R2483 T4122 T4121 compound mosaic,morphology
R2484 T4124 T4123 auxpass was,reflected
R2485 T4125 T4123 prep at,reflected
R2486 T4126 T4127 det the,level
R2487 T4127 T4125 pobj level,at
R2488 T4128 T4127 prep of,level
R2489 T4129 T4130 compound Tg,protein
R2490 T4130 T4128 pobj protein,of
R2491 T4131 T4130 compound Snail,protein
R2492 T4132 T4123 punct ", ",reflected
R2493 T4133 T4134 mark with,expressing
R2494 T4134 T4123 advcl expressing,reflected
R2495 T4135 T4136 advmod only,regions
R2496 T4136 T4134 nsubj regions,expressing
R2497 T4137 T4136 det the,regions
R2498 T4138 T4136 amod hyperthickened,regions
R2499 T4139 T4140 amod nuclear,Snail
R2500 T4140 T4134 dobj Snail,expressing
R2501 T4141 T4142 npadvmod HA,tagged
R2502 T4142 T4140 amod tagged,Snail
R2503 T4143 T4142 punct -,tagged
R2504 T4144 T4145 punct (,2C
R2505 T4145 T4123 parataxis 2C,reflected
R2506 T4146 T4145 compound Figure,2C
R2507 T4147 T4145 punct ),2C
R2508 T4148 T4123 punct .,reflected
R2509 T4150 T4151 det These,areas
R2510 T4151 T4153 nsubjpass areas,marked
R2511 T4152 T4151 amod hyperthickened,areas
R2512 T4154 T4153 auxpass were,marked
R2513 T4155 T4153 agent by,marked
R2514 T4156 T4157 amod excessive,proliferation
R2515 T4157 T4155 pobj proliferation,by
R2516 T4158 T4153 punct ", ",marked
R2517 T4159 T4160 mark as,revealed
R2518 T4160 T4153 advcl revealed,marked
R2519 T4161 T4160 agent by,revealed
R2520 T4162 T4161 pobj antibodies,by
R2521 T4163 T4162 prep against,antibodies
R2522 T4164 T4165 det the,antigen
R2523 T4165 T4163 pobj antigen,against
R2524 T4166 T4165 amod proliferating,antigen
R2525 T4167 T4165 amod nuclear,antigen
R2526 T4168 T4165 appos Ki67,antigen
R2527 T4169 T4170 punct (,2D
R2528 T4170 T4153 parataxis 2D,marked
R2529 T4171 T4170 compound Figure,2D
R2530 T4172 T4170 cc and,2D
R2531 T4173 T4170 conj 2E,2D
R2532 T4174 T4170 punct ),2D
R2533 T4175 T4153 punct .,marked
R2534 T4177 T4178 amod Activated,cells
R2535 T4178 T4183 nsubj cells,were
R2536 T4179 T4178 punct ", ",cells
R2537 T4180 T4181 npadvmod pMAPK,positive
R2538 T4181 T4178 amod positive,cells
R2539 T4182 T4181 punct -,positive
R2540 T4184 T4183 advmod also,were
R2541 T4185 T4183 acomp prevalent,were
R2542 T4186 T4183 prep in,were
R2543 T4187 T4188 det these,areas
R2544 T4188 T4186 pobj areas,in
R2545 T4189 T4190 punct (,2F
R2546 T4190 T4183 parataxis 2F,were
R2547 T4191 T4190 compound Figure,2F
R2548 T4192 T4190 cc and,2F
R2549 T4193 T4190 conj 2G,2F
R2550 T4194 T4190 punct ),2F
R2551 T4195 T4183 punct ", ",were
R2552 T4196 T4197 mark as,were
R2553 T4197 T4183 advcl were,were
R2554 T4198 T4197 nsubj cells,were
R2555 T4199 T4198 acl expressing,cells
R2556 T4200 T4199 dobj keratin,expressing
R2557 T4201 T4200 nummod 6,keratin
R2558 T4202 T4200 punct ", ",keratin
R2559 T4203 T4204 det a,keratin
R2560 T4204 T4200 appos keratin,keratin
R2561 T4205 T4204 acl induced,keratin
R2562 T4206 T4205 prep in,induced
R2563 T4207 T4208 det the,layers
R2564 T4208 T4206 pobj layers,in
R2565 T4209 T4208 amod suprabasal,layers
R2566 T4210 T4208 prep of,layers
R2567 T4211 T4212 amod hyperproliferative,skin
R2568 T4212 T4210 pobj skin,of
R2569 T4213 T4214 punct (,2H
R2570 T4214 T4183 parataxis 2H,were
R2571 T4215 T4214 compound Figure,2H
R2572 T4216 T4214 cc and,2H
R2573 T4217 T4214 conj 2I,2H
R2574 T4218 T4214 punct ),2H
R2575 T4219 T4183 punct .,were
R2576 T4221 T4222 nsubj Expression,block
R2577 T4223 T4221 prep of,Expression
R2578 T4224 T4225 det the,transgene
R2579 T4225 T4223 pobj transgene,of
R2580 T4226 T4225 compound Snail,transgene
R2581 T4227 T4222 aux did,block
R2582 T4228 T4222 neg not,block
R2583 T4229 T4230 amod terminal,differentiation
R2584 T4230 T4222 dobj differentiation,block
R2585 T4231 T4222 prep in,block
R2586 T4232 T4233 det the,epidermis
R2587 T4233 T4231 pobj epidermis,in
R2588 T4234 T4233 amod hyperproliferative,epidermis
R2589 T4235 T4222 punct ", ",block
R2590 T4236 T4222 cc but,block
R2591 T4237 T4238 nsubj it,distorted
R2592 T4238 T4222 conj distorted,block
R2593 T4239 T4238 dobj it,distorted
R2594 T4240 T4238 advmod markedly,distorted
R2595 T4241 T4242 punct (,3A
R2596 T4242 T4238 parataxis 3A,distorted
R2597 T4243 T4242 compound Figure,3A
R2598 T4244 T4245 punct –,3H
R2599 T4245 T4242 prep 3H,3A
R2600 T4246 T4242 punct ),3A
R2601 T4247 T4238 punct .,distorted
R2602 T4249 T4250 advcl Typical,led
R2603 T4251 T4249 prep of,Typical
R2604 T4252 T4253 amod most,conditions
R2605 T4253 T4251 pobj conditions,of
R2606 T4254 T4253 compound hyperproliferating,conditions
R2607 T4255 T4250 punct ", ",led
R2608 T4256 T4257 compound Snail,expression
R2609 T4257 T4250 nsubj expression,led
R2610 T4258 T4250 prep to,led
R2611 T4259 T4260 det a,expansion
R2612 T4260 T4258 pobj expansion,to
R2613 T4261 T4260 amod large,expansion
R2614 T4262 T4250 prep in,led
R2615 T4263 T4262 pobj layers,in
R2616 T4264 T4263 prep with,layers
R2617 T4265 T4266 amod spinous,morphology
R2618 T4266 T4264 pobj morphology,with
R2619 T4267 T4265 cc and,spinous
R2620 T4268 T4265 conj granular,spinous
R2621 T4269 T4250 punct .,led
R2622 T4271 T4272 advmod Additionally,was
R2623 T4273 T4272 punct ", ",was
R2624 T4274 T4272 advmod however,was
R2625 T4275 T4272 punct ", ",was
R2626 T4276 T4277 det a,expansion
R2627 T4277 T4272 attr expansion,was
R2628 T4278 T4277 amod marked,expansion
R2629 T4279 T4278 cc and,marked
R2630 T4280 T4278 conj variable,marked
R2631 T4281 T4277 prep of,expansion
R2632 T4282 T4281 pobj keratin,of
R2633 T4283 T4282 nummod 5,keratin
R2634 T4284 T4282 punct (,keratin
R2635 T4285 T4282 appos K5,keratin
R2636 T4286 T4282 punct ),keratin
R2637 T4287 T4282 punct ", ",keratin
R2638 T4288 T4289 advmod normally,restricted
R2639 T4289 T4282 acl restricted,keratin
R2640 T4290 T4289 prep to,restricted
R2641 T4291 T4292 det the,layer
R2642 T4292 T4290 pobj layer,to
R2643 T4293 T4292 amod innermost,layer
R2644 T4294 T4292 amod basal,layer
R2645 T4295 T4296 punct (,see
R2646 T4296 T4272 parataxis see,was
R2647 T4297 T4296 dobj Figure,see
R2648 T4298 T4297 nummod 3,Figure
R2649 T4299 T4296 punct ),see
R2650 T4300 T4272 punct .,was
R2651 T4302 T4303 mark Although,precluded
R2652 T4303 T4318 advcl precluded,emerged
R2653 T4304 T4305 det the,failure
R2654 T4305 T4303 nsubj failure,precluded
R2655 T4306 T4305 prep of,failure
R2656 T4307 T4308 nmod Snail,mice
R2657 T4308 T4306 pobj mice,of
R2658 T4309 T4307 punct -,Snail
R2659 T4310 T4307 amod null,Snail
R2660 T4311 T4312 aux to,develop
R2661 T4312 T4305 acl develop,failure
R2662 T4313 T4314 amod past,gastrulation
R2663 T4314 T4312 dobj gastrulation,develop
R2664 T4315 T4316 punct [,21
R2665 T4316 T4305 parataxis 21,failure
R2666 T4317 T4316 punct ],21
R2667 T4319 T4320 poss our,ability
R2668 T4320 T4303 dobj ability,precluded
R2669 T4321 T4322 aux to,study
R2670 T4322 T4320 acl study,ability
R2671 T4323 T4324 det the,loss
R2672 T4324 T4322 dobj loss,study
R2673 T4325 T4324 prep of,loss
R2674 T4326 T4327 compound Snail,function
R2675 T4327 T4325 pobj function,of
R2676 T4328 T4324 prep in,loss
R2677 T4329 T4330 compound skin,development
R2678 T4330 T4328 pobj development,in
R2679 T4331 T4318 punct ", ",emerged
R2680 T4332 T4333 det a,correlation
R2681 T4333 T4318 nsubj correlation,emerged
R2682 T4334 T4333 amod good,correlation
R2683 T4335 T4318 prep between,emerged
R2684 T4336 T4337 det the,expression
R2685 T4337 T4335 pobj expression,between
R2686 T4338 T4337 prep of,expression
R2687 T4339 T4340 compound Snail,protein
R2688 T4340 T4338 pobj protein,of
R2689 T4341 T4337 cc and,expression
R2690 T4342 T4343 det the,extension
R2691 T4343 T4337 conj extension,expression
R2692 T4344 T4343 prep of,extension
R2693 T4345 T4344 pobj K5,of
R2694 T4346 T4345 punct ", ",K5
R2695 T4347 T4345 conj Ki67,K5
R2696 T4348 T4347 punct ", ",Ki67
R2697 T4349 T4347 cc and,Ki67
R2698 T4350 T4347 conj pMAPK,Ki67
R2699 T4351 T4343 advmod suprabasally,extension
R2700 T4352 T4353 punct (,compare
R2701 T4353 T4318 parataxis compare,emerged
R2702 T4354 T4353 dobj data,compare
R2703 T4355 T4353 prep in,compare
R2704 T4356 T4357 nmod Figures,2
R2705 T4357 T4355 pobj 2,in
R2706 T4358 T4357 cc and,2
R2707 T4359 T4357 conj 3,2
R2708 T4360 T4353 punct ),compare
R2709 T4361 T4318 punct .,emerged
R2710 T4363 T4364 det The,changes
R2711 T4364 T4365 nsubjpass changes,accompanied
R2712 T4366 T4364 prep in,changes
R2713 T4367 T4366 pobj hyperproliferation,in
R2714 T4368 T4367 cc and,hyperproliferation
R2715 T4369 T4367 conj differentiation,hyperproliferation
R2716 T4370 T4365 auxpass were,accompanied
R2717 T4371 T4365 neg not,accompanied
R2718 T4372 T4365 advmod initially,accompanied
R2719 T4373 T4365 agent by,accompanied
R2720 T4374 T4375 amod gross,signs
R2721 T4375 T4373 pobj signs,by
R2722 T4376 T4375 prep of,signs
R2723 T4377 T4378 amod epithelial,invaginations
R2724 T4378 T4376 pobj invaginations,of
R2725 T4379 T4365 punct .,accompanied
R2726 T4381 T4382 prep With,developed
R2727 T4383 T4381 pobj age,With
R2728 T4384 T4382 punct ", ",developed
R2729 T4385 T4382 advmod however,developed
R2730 T4386 T4382 punct ", ",developed
R2731 T4387 T4388 amod epidermal,folds
R2732 T4388 T4382 nsubj folds,developed
R2733 T4389 T4388 cc and,folds
R2734 T4390 T4388 conj undulations,folds
R2735 T4391 T4382 prep in,developed
R2736 T4392 T4391 pobj areas,in
R2737 T4393 T4394 advmod where,expressed
R2738 T4394 T4392 relcl expressed,areas
R2739 T4395 T4394 nsubjpass Snail,expressed
R2740 T4396 T4394 auxpass was,expressed
R2741 T4397 T4382 punct ", ",developed
R2742 T4398 T4382 cc and,developed
R2743 T4399 T4400 amod proliferative,markers
R2744 T4400 T4401 nsubj markers,persisted
R2745 T4401 T4382 conj persisted,developed
R2746 T4402 T4401 prep in,persisted
R2747 T4403 T4404 det these,regions
R2748 T4404 T4402 pobj regions,in
R2749 T4405 T4406 punct (,staining
R2750 T4406 T4401 parataxis staining,persisted
R2751 T4407 T4408 compound Figure,3I
R2752 T4408 T4406 dep 3I,staining
R2753 T4409 T4408 cc and,3I
R2754 T4410 T4408 conj 3J,3I
R2755 T4411 T4406 punct ;,staining
R2756 T4412 T4413 amod anti-cyclin,D
R2757 T4413 T4406 compound D,staining
R2758 T4414 T4406 punct ),staining
R2759 T4415 T4382 punct .,developed
R2760 T4417 T4418 det The,undulations
R2761 T4418 T4419 nsubjpass undulations,accompanied
R2762 T4420 T4419 auxpass were,accompanied
R2763 T4421 T4419 agent by,accompanied
R2764 T4422 T4423 amod partial,dissolution
R2765 T4423 T4421 pobj dissolution,by
R2766 T4424 T4423 prep of,dissolution
R2767 T4425 T4426 det the,membrane
R2768 T4426 T4424 pobj membrane,of
R2769 T4427 T4426 amod underlying,membrane
R2770 T4428 T4426 compound basement,membrane
R2771 T4429 T4430 punct (,3K
R2772 T4430 T4419 parataxis 3K,accompanied
R2773 T4431 T4430 compound Figure,3K
R2774 T4432 T4430 cc and,3K
R2775 T4433 T4430 conj 3L,3K
R2776 T4434 T4430 punct ),3K
R2777 T4435 T4419 punct .,accompanied
R2778 T4437 T4438 amod Aberrant,staining
R2779 T4438 T4439 nsubjpass staining,observed
R2780 T4440 T4439 auxpass was,observed
R2781 T4441 T4439 advmod also,observed
R2782 T4442 T4439 prep with,observed
R2783 T4443 T4442 pobj antibodies,with
R2784 T4444 T4443 prep against,antibodies
R2785 T4445 T4444 pobj components,against
R2786 T4446 T4445 prep of,components
R2787 T4447 T4448 det the,hemidesmosomes
R2788 T4448 T4446 pobj hemidesmosomes,of
R2789 T4449 T4445 punct ", ",components
R2790 T4450 T4451 dep which,provide
R2791 T4451 T4445 relcl provide,components
R2792 T4452 T4453 amod strong,adhesion
R2793 T4453 T4451 dobj adhesion,provide
R2794 T4454 T4453 prep of,adhesion
R2795 T4455 T4456 amod basal,cells
R2796 T4456 T4454 pobj cells,of
R2797 T4457 T4456 amod epidermal,cells
R2798 T4458 T4451 prep to,provide
R2799 T4459 T4460 det the,lamina
R2800 T4460 T4458 pobj lamina,to
R2801 T4461 T4460 amod underlying,lamina
R2802 T4462 T4460 amod basal,lamina
R2803 T4463 T4464 punct (,3M
R2804 T4464 T4439 parataxis 3M,observed
R2805 T4465 T4464 compound Figure,3M
R2806 T4466 T4464 cc and,3M
R2807 T4467 T4464 conj 3N,3M
R2808 T4468 T4464 punct ),3M
R2809 T4469 T4439 punct .,observed
R2810 T4471 T4472 advmod Interestingly,occur
R2811 T4473 T4472 punct ", ",occur
R2812 T4474 T4475 amod similar,types
R2813 T4475 T4472 nsubj types,occur
R2814 T4476 T4475 prep of,types
R2815 T4477 T4476 pobj alterations,of
R2816 T4478 T4472 prep in,occur
R2817 T4479 T4480 det the,membrane
R2818 T4480 T4478 pobj membrane,in
R2819 T4481 T4480 compound basement,membrane
R2820 T4482 T4472 prep in,occur
R2821 T4483 T4484 det the,bud
R2822 T4484 T4482 pobj bud,in
R2823 T4485 T4484 compound hair,bud
R2824 T4486 T4484 prep of,bud
R2825 T4487 T4488 amod embryonic,mice
R2826 T4488 T4486 pobj mice,of
R2827 T4489 T4487 cc and,embryonic
R2828 T4490 T4487 conj newborn,embryonic
R2829 T4491 T4492 advmod when,expressed
R2830 T4492 T4472 advcl expressed,occur
R2831 T4493 T4492 nsubjpass Snail,expressed
R2832 T4494 T4492 auxpass is,expressed
R2833 T4495 T4492 advmod normally,expressed
R2834 T4496 T4472 punct .,occur
R2835 T4498 T4499 det The,fact
R2836 T4499 T4500 nsubj fact,suggests
R2837 T4501 T4502 mark that,altered
R2838 T4502 T4499 acl altered,fact
R2839 T4503 T4504 det the,membrane
R2840 T4504 T4502 nsubjpass membrane,altered
R2841 T4505 T4504 compound basement,membrane
R2842 T4506 T4504 acl separating,membrane
R2843 T4507 T4508 det the,epidermis
R2844 T4508 T4506 dobj epidermis,separating
R2845 T4509 T4506 prep from,separating
R2846 T4510 T4511 det the,dermis
R2847 T4511 T4509 pobj dermis,from
R2848 T4512 T4502 auxpass is,altered
R2849 T4513 T4514 advmod only,in
R2850 T4514 T4502 prep in,altered
R2851 T4515 T4516 det the,animals
R2852 T4516 T4514 pobj animals,in
R2853 T4517 T4516 amod adult,animals
R2854 T4518 T4516 compound Tg,animals
R2855 T4519 T4520 det the,involvement
R2856 T4520 T4500 dobj involvement,suggests
R2857 T4521 T4520 prep of,involvement
R2858 T4522 T4523 amod intermediary,factors
R2859 T4523 T4521 pobj factors,of
R2860 T4524 T4525 neg not,available
R2861 T4525 T4523 relcl available,factors
R2862 T4526 T4527 advmod as,readily
R2863 T4527 T4525 advmod readily,available
R2864 T4528 T4525 prep in,available
R2865 T4529 T4530 det the,epidermis
R2866 T4530 T4528 pobj epidermis,in
R2867 T4531 T4532 mark as,are
R2868 T4532 T4525 advcl are,available
R2869 T4533 T4532 nsubj they,are
R2870 T4534 T4532 prep in,are
R2871 T4535 T4536 det the,follicle
R2872 T4536 T4534 pobj follicle,in
R2873 T4537 T4500 punct .,suggests
R2878 T5218 T5219 amod Possible,Links
R2879 T5220 T5219 prep between,Links
R2880 T5221 T5222 amod Epidermal,Hyperproliferation
R2881 T5222 T5220 pobj Hyperproliferation,between
R2882 T5223 T5222 cc and,Hyperproliferation
R2883 T5224 T5225 amod Down,regulation
R2884 T5225 T5222 conj regulation,Hyperproliferation
R2885 T5226 T5225 punct -,regulation
R2886 T5227 T5225 prep of,regulation
R2887 T5228 T5229 compound AJ,Proteins
R2888 T5229 T5227 pobj Proteins,of
R2889 T5230 T5219 prep in,Links
R2890 T5231 T5232 compound Snail,Mice
R2891 T5232 T5230 pobj Mice,in
R2892 T5233 T5232 compound Tg,Mice
R2893 T5235 T5236 prep Given,examined
R2894 T5237 T5238 mark that,is
R2895 T5238 T5235 pcomp is,Given
R2896 T5239 T5240 det the,promoter
R2897 T5240 T5238 nsubj promoter,is
R2898 T5241 T5242 compound E,cadherin
R2899 T5242 T5240 compound cadherin,promoter
R2900 T5243 T5242 punct -,cadherin
R2901 T5244 T5245 det a,target
R2902 T5245 T5238 attr target,is
R2903 T5246 T5245 amod direct,target
R2904 T5247 T5245 prep for,target
R2905 T5248 T5249 npadvmod Snail,mediated
R2906 T5249 T5251 amod mediated,repression
R2907 T5250 T5249 punct -,mediated
R2908 T5251 T5247 pobj repression,for
R2909 T5252 T5253 advmod in,vitro
R2910 T5253 T5238 advmod vitro,is
R2911 T5254 T5255 punct [,13
R2912 T5255 T5238 parataxis 13,is
R2913 T5256 T5255 nummod 4,13
R2914 T5257 T5255 punct ",",13
R2915 T5258 T5255 nummod 12,13
R2916 T5259 T5255 punct ",",13
R2917 T5260 T5255 punct ],13
R2918 T5261 T5238 punct ", ",is
R2919 T5262 T5238 cc and,is
R2920 T5263 T5264 mark that,regulated
R2921 T5264 T5238 conj regulated,is
R2922 T5265 T5266 compound E,cadherin
R2923 T5266 T5264 nsubjpass cadherin,regulated
R2924 T5267 T5266 punct -,cadherin
R2925 T5268 T5264 auxpass was,regulated
R2926 T5269 T5264 advmod down,regulated
R2927 T5270 T5264 punct -,regulated
R2928 T5271 T5264 prep in,regulated
R2929 T5272 T5273 npadvmod Snail,expressing
R2930 T5273 T5275 amod expressing,buds
R2931 T5274 T5273 punct -,expressing
R2932 T5275 T5271 pobj buds,in
R2933 T5276 T5275 compound hair,buds
R2934 T5277 T5236 punct ", ",examined
R2935 T5278 T5236 nsubj we,examined
R2936 T5279 T5280 det the,status
R2937 T5280 T5236 dobj status,examined
R2938 T5281 T5280 prep of,status
R2939 T5282 T5283 compound E,cadherin
R2940 T5283 T5281 pobj cadherin,of
R2941 T5284 T5283 punct -,cadherin
R2942 T5285 T5283 cc and,cadherin
R2943 T5286 T5287 amod other,proteins
R2944 T5287 T5283 conj proteins,cadherin
R2945 T5288 T5287 compound AJ,proteins
R2946 T5289 T5236 prep within,examined
R2947 T5290 T5289 pobj regions,within
R2948 T5291 T5290 prep of,regions
R2949 T5292 T5293 amod hyperproliferative,epidermis
R2950 T5293 T5291 pobj epidermis,of
R2951 T5294 T5295 advmod where,was
R2952 T5295 T5290 relcl was,regions
R2953 T5296 T5297 compound Tg,Snail
R2954 T5297 T5295 nsubj Snail,was
R2955 T5298 T5295 acomp present,was
R2956 T5299 T5300 punct (,4A
R2957 T5300 T5236 parataxis 4A,examined
R2958 T5301 T5300 compound Figure,4A
R2959 T5302 T5300 punct ),4A
R2960 T5303 T5236 punct .,examined
R2961 T5305 T5306 prep In,diminished
R2962 T5307 T5308 det these,regions
R2963 T5308 T5305 pobj regions,In
R2964 T5309 T5306 punct ", ",diminished
R2965 T5310 T5311 compound immunofluorescence,staining
R2966 T5311 T5306 nsubjpass staining,diminished
R2967 T5312 T5311 prep of,staining
R2968 T5313 T5314 compound E,cadherin
R2969 T5314 T5312 pobj cadherin,of
R2970 T5315 T5314 punct -,cadherin
R2971 T5316 T5314 cc and,cadherin
R2972 T5317 T5318 compound α,catenin
R2973 T5318 T5314 conj catenin,cadherin
R2974 T5319 T5318 punct -,catenin
R2975 T5320 T5306 auxpass were,diminished
R2976 T5321 T5306 advmod markedly,diminished
R2977 T5322 T5306 punct .,diminished
R2978 T5324 T5325 prep In,was
R2979 T5326 T5324 pobj contrast,In
R2980 T5327 T5325 punct ", ",was
R2981 T5328 T5329 det the,intensity
R2982 T5329 T5325 nsubj intensity,was
R2983 T5330 T5329 prep of,intensity
R2984 T5331 T5332 compound antibody,staining
R2985 T5332 T5330 pobj staining,of
R2986 T5333 T5329 prep for,intensity
R2987 T5334 T5335 nummod two,proteins
R2988 T5335 T5333 pobj proteins,for
R2989 T5336 T5335 amod other,proteins
R2990 T5337 T5335 compound AJ,proteins
R2991 T5338 T5335 punct ", ",proteins
R2992 T5339 T5340 compound β,catenin
R2993 T5340 T5335 appos catenin,proteins
R2994 T5341 T5340 punct -,catenin
R2995 T5342 T5340 cc and,catenin
R2996 T5343 T5340 conj Ajuba,catenin
R2997 T5344 T5325 punct ", ",was
R2998 T5345 T5325 advmod still,was
R2999 T5346 T5325 acomp strong,was
R3000 T5347 T5325 punct .,was
R3001 T5349 T5350 advmod Interestingly,appeared
R3002 T5351 T5350 punct ", ",appeared
R3003 T5352 T5350 advmod however,appeared
R3004 T5353 T5350 punct ", ",appeared
R3005 T5354 T5350 prep despite,appeared
R3006 T5355 T5356 amod appreciable,immunofluorescence
R3007 T5356 T5354 pobj immunofluorescence,despite
R3008 T5357 T5350 punct ", ",appeared
R3009 T5358 T5350 nsubj localization,appeared
R3010 T5359 T5358 prep of,localization
R3011 T5360 T5361 compound β,catenin
R3012 T5361 T5359 pobj catenin,of
R3013 T5362 T5361 punct -,catenin
R3014 T5363 T5361 cc and,catenin
R3015 T5364 T5361 conj Ajuba,catenin
R3016 T5365 T5366 aux to,be
R3017 T5366 T5350 xcomp be,appeared
R3018 T5367 T5368 advmod largely,cytoplasmic
R3019 T5368 T5366 acomp cytoplasmic,be
R3020 T5369 T5370 advmod rather,than
R3021 T5370 T5368 cc than,cytoplasmic
R3022 T5371 T5368 conj at,cytoplasmic
R3023 T5372 T5373 compound cell,cell
R3024 T5373 T5375 compound cell,borders
R3025 T5374 T5373 punct -,cell
R3026 T5375 T5371 pobj borders,at
R3027 T5376 T5377 punct (,insets
R3028 T5377 T5350 parataxis insets,appeared
R3029 T5378 T5379 compound Figure,4A
R3030 T5379 T5377 compound 4A,insets
R3031 T5380 T5377 punct ),insets
R3032 T5381 T5350 punct .,appeared
R3033 T5383 T5384 amod Architectural,differences
R3034 T5384 T5385 nsubj differences,made
R3035 T5386 T5384 prep in,differences
R3036 T5387 T5388 det the,epidermis
R3037 T5388 T5386 pobj epidermis,in
R3038 T5389 T5390 compound Western,blot
R3039 T5390 T5391 compound blot,analyses
R3040 T5391 T5392 nsubj analyses,difficult
R3041 T5392 T5385 ccomp difficult,made
R3042 T5393 T5392 advmod somewhat,difficult
R3043 T5394 T5395 aux to,gauge
R3044 T5395 T5392 advcl gauge,difficult
R3045 T5396 T5385 punct .,made
R3046 T5398 T5399 advmod However,accentuated
R3047 T5400 T5399 punct ", ",accentuated
R3048 T5401 T5399 prep in,accentuated
R3049 T5402 T5401 pobj regions,in
R3050 T5403 T5404 amod such,as
R3051 T5404 T5402 prep as,regions
R3052 T5405 T5406 compound ear,skin
R3053 T5406 T5404 pobj skin,as
R3054 T5407 T5402 punct ", ",regions
R3055 T5408 T5409 advmod where,expressed
R3056 T5409 T5402 relcl expressed,regions
R3057 T5410 T5411 det the,levels
R3058 T5411 T5409 nsubjpass levels,expressed
R3059 T5412 T5411 amod highest,levels
R3060 T5413 T5411 prep of,levels
R3061 T5414 T5415 compound Snail,protein
R3062 T5415 T5413 pobj protein,of
R3063 T5416 T5409 auxpass were,expressed
R3064 T5417 T5399 punct ", ",accentuated
R3065 T5418 T5419 det the,effects
R3066 T5419 T5399 nsubjpass effects,accentuated
R3067 T5420 T5399 auxpass were,accentuated
R3068 T5421 T5399 punct .,accentuated
R3069 T5423 T5424 prep In,reduced
R3070 T5425 T5426 preconj both,skin
R3071 T5426 T5423 pobj skin,In
R3072 T5427 T5426 compound back,skin
R3073 T5428 T5426 cc and,skin
R3074 T5429 T5430 compound ear,skin
R3075 T5430 T5426 conj skin,skin
R3076 T5431 T5424 punct ", ",reduced
R3077 T5432 T5433 amod overall,levels
R3078 T5433 T5424 nsubjpass levels,reduced
R3079 T5434 T5433 prep of,levels
R3080 T5435 T5436 compound E,cadherin
R3081 T5436 T5434 pobj cadherin,of
R3082 T5437 T5436 punct -,cadherin
R3083 T5438 T5436 cc and,cadherin
R3084 T5439 T5440 compound α,catenin
R3085 T5440 T5436 conj catenin,cadherin
R3086 T5441 T5440 punct -,catenin
R3087 T5442 T5424 auxpass were,reduced
R3088 T5443 T5424 punct ", ",reduced
R3089 T5444 T5424 prep under,reduced
R3090 T5445 T5444 pobj conditions,under
R3091 T5446 T5447 advmod where,remained
R3092 T5447 T5445 relcl remained,conditions
R3093 T5448 T5449 nmod β,catenin
R3094 T5449 T5451 nmod catenin,levels
R3095 T5450 T5449 punct -,catenin
R3096 T5451 T5447 nsubj levels,remained
R3097 T5452 T5449 cc and,catenin
R3098 T5453 T5449 conj Ajuba,catenin
R3099 T5454 T5447 acomp unchanged,remained
R3100 T5455 T5447 advcl relative,remained
R3101 T5456 T5455 prep to,relative
R3102 T5457 T5456 pobj controls,to
R3103 T5458 T5459 punct (,4B
R3104 T5459 T5424 parataxis 4B,reduced
R3105 T5460 T5459 compound Figure,4B
R3106 T5461 T5459 punct ),4B
R3107 T5462 T5424 punct .,reduced
R3108 T5464 T5465 advcl Taken,were
R3109 T5466 T5464 advmod together,Taken
R3110 T5467 T5465 punct ", ",were
R3111 T5468 T5469 det these,data
R3112 T5469 T5465 nsubj data,were
R3113 T5470 T5465 acomp consistent,were
R3114 T5471 T5470 prep with,consistent
R3115 T5472 T5473 poss our,results
R3116 T5473 T5471 pobj results,with
R3117 T5474 T5473 acl obtained,results
R3118 T5475 T5474 prep from,obtained
R3119 T5476 T5477 compound immunofluorescence,microscopy
R3120 T5477 T5475 pobj microscopy,from
R3121 T5478 T5465 punct .,were
R3122 T5480 T5481 advmod A,priori
R3123 T5481 T5482 advmod priori,be
R3124 T5483 T5482 punct ", ",be
R3125 T5484 T5485 det the,decrease
R3126 T5485 T5482 nsubj decrease,be
R3127 T5486 T5485 prep in,decrease
R3128 T5487 T5488 compound α,catenin
R3129 T5488 T5490 compound catenin,levels
R3130 T5489 T5488 punct -,catenin
R3131 T5490 T5486 pobj levels,in
R3132 T5491 T5482 aux could,be
R3133 T5492 T5482 prep due,be
R3134 T5493 T5492 pcomp to,due
R3135 T5494 T5495 preconj either,repression
R3136 T5495 T5492 pobj repression,due
R3137 T5496 T5495 amod direct,repression
R3138 T5497 T5495 amod transcriptional,repression
R3139 T5498 T5495 prep by,repression
R3140 T5499 T5498 pobj Snail,by
R3141 T5500 T5495 cc or,repression
R3142 T5501 T5495 conj perturbations,repression
R3143 T5502 T5501 prep in,perturbations
R3144 T5503 T5504 compound AJ,formation
R3145 T5504 T5502 pobj formation,in
R3146 T5505 T5501 acl caused,perturbations
R3147 T5506 T5505 agent by,caused
R3148 T5507 T5508 det the,decrease
R3149 T5508 T5506 pobj decrease,by
R3150 T5509 T5508 prep in,decrease
R3151 T5510 T5511 compound E,cadherin
R3152 T5511 T5513 compound cadherin,expression
R3153 T5512 T5511 punct -,cadherin
R3154 T5513 T5509 pobj expression,in
R3155 T5514 T5513 compound gene,expression
R3156 T5515 T5482 punct .,be
R3157 T5517 T5518 aux To,distinguish
R3158 T5518 T5519 advcl distinguish,tested
R3159 T5520 T5518 prep between,distinguish
R3160 T5521 T5522 det these,possibilities
R3161 T5522 T5520 pobj possibilities,between
R3162 T5523 T5519 punct ", ",tested
R3163 T5524 T5519 nsubj we,tested
R3164 T5525 T5526 mark whether,restored
R3165 T5526 T5519 ccomp restored,tested
R3166 T5527 T5528 compound α,catenin
R3167 T5528 T5530 compound catenin,levels
R3168 T5529 T5528 punct -,catenin
R3169 T5530 T5526 nsubjpass levels,restored
R3170 T5531 T5526 aux could,restored
R3171 T5532 T5526 auxpass be,restored
R3172 T5533 T5526 agent by,restored
R3173 T5534 T5535 amod exogenous,expression
R3174 T5535 T5533 pobj expression,by
R3175 T5536 T5535 prep of,expression
R3176 T5537 T5538 compound E,cadherin
R3177 T5538 T5536 pobj cadherin,of
R3178 T5539 T5538 punct -,cadherin
R3179 T5540 T5526 prep in,restored
R3180 T5541 T5542 npadvmod Snail,expressing
R3181 T5542 T5544 amod expressing,keratinocytes
R3182 T5543 T5542 punct -,expressing
R3183 T5544 T5540 pobj keratinocytes,in
R3184 T5545 T5519 punct .,tested
R3185 T5547 T5548 mark As,shown
R3186 T5548 T5549 advcl shown,displayed
R3187 T5550 T5548 prep in,shown
R3188 T5551 T5552 compound Figure,4C
R3189 T5552 T5550 pobj 4C,in
R3190 T5553 T5549 punct ", ",displayed
R3191 T5554 T5555 advmod transiently,transfected
R3192 T5555 T5556 amod transfected,keratinocytes
R3193 T5556 T5549 nsubj keratinocytes,displayed
R3194 T5557 T5556 acl expressing,keratinocytes
R3195 T5558 T5559 npadvmod HA,tagged
R3196 T5559 T5561 amod tagged,Snail
R3197 T5560 T5559 punct -,tagged
R3198 T5561 T5557 dobj Snail,expressing
R3199 T5562 T5563 det a,loss
R3200 T5563 T5549 dobj loss,displayed
R3201 T5564 T5563 prep of,loss
R3202 T5565 T5566 compound E,cadherin
R3203 T5566 T5564 pobj cadherin,of
R3204 T5567 T5566 punct -,cadherin
R3205 T5568 T5566 cc and,cadherin
R3206 T5569 T5570 compound α,catenin
R3207 T5570 T5566 conj catenin,cadherin
R3208 T5571 T5570 punct -,catenin
R3209 T5572 T5549 prep at,displayed
R3210 T5573 T5574 compound cell,cell
R3211 T5574 T5576 compound cell,borders
R3212 T5575 T5574 punct -,cell
R3213 T5576 T5572 pobj borders,at
R3214 T5577 T5549 punct .,displayed
R3215 T5579 T5580 nsubj Coexpression,enabled
R3216 T5581 T5579 prep of,Coexpression
R3217 T5582 T5583 amod exogenous,cadherin
R3218 T5583 T5581 pobj cadherin,of
R3219 T5584 T5585 npadvmod HA,tagged
R3220 T5585 T5583 amod tagged,cadherin
R3221 T5586 T5585 punct -,tagged
R3222 T5587 T5583 compound E,cadherin
R3223 T5588 T5583 punct -,cadherin
R3224 T5589 T5580 preconj not,enabled
R3225 T5590 T5589 advmod only,not
R3226 T5591 T5592 compound cell,cell
R3227 T5592 T5594 compound cell,localization
R3228 T5593 T5592 punct -,cell
R3229 T5594 T5580 dobj localization,enabled
R3230 T5595 T5594 compound border,localization
R3231 T5596 T5594 prep of,localization
R3232 T5597 T5598 compound E,cadherin
R3233 T5598 T5600 compound cadherin,protein
R3234 T5599 T5598 punct -,cadherin
R3235 T5600 T5596 pobj protein,of
R3236 T5601 T5580 punct ", ",enabled
R3237 T5602 T5580 cc but,enabled
R3238 T5603 T5602 advmod also,but
R3239 T5604 T5580 conj rescued,enabled
R3240 T5605 T5606 det the,staining
R3241 T5606 T5604 dobj staining,rescued
R3242 T5607 T5608 compound cell,cell
R3243 T5608 T5606 compound cell,staining
R3244 T5609 T5608 punct -,cell
R3245 T5610 T5606 compound border,staining
R3246 T5611 T5606 prep of,staining
R3247 T5612 T5613 compound α,catenin
R3248 T5613 T5611 pobj catenin,of
R3249 T5614 T5613 punct -,catenin
R3250 T5615 T5616 punct (,4C
R3251 T5616 T5604 parataxis 4C,rescued
R3252 T5617 T5616 compound Figure,4C
R3253 T5618 T5616 punct ),4C
R3254 T5619 T5580 punct .,enabled
R3255 T5621 T5622 det The,ability
R3256 T5622 T5623 nsubj ability,argues
R3257 T5624 T5625 aux to,restore
R3258 T5625 T5622 acl restore,ability
R3259 T5626 T5627 compound α,catenin
R3260 T5627 T5625 dobj catenin,restore
R3261 T5628 T5627 punct -,catenin
R3262 T5629 T5627 appos expression,catenin
R3263 T5630 T5629 cc and,expression
R3264 T5631 T5629 conj localization,expression
R3265 T5632 T5625 prep under,restore
R3266 T5633 T5634 det these,conditions
R3267 T5634 T5632 pobj conditions,under
R3268 T5635 T5623 prep against,argues
R3269 T5636 T5637 det the,notion
R3270 T5637 T5635 pobj notion,against
R3271 T5638 T5639 mark that,represses
R3272 T5639 T5637 acl represses,notion
R3273 T5640 T5639 nsubj Snail,represses
R3274 T5641 T5639 advmod transcriptionally,represses
R3275 T5642 T5643 compound α,catenin
R3276 T5643 T5639 dobj catenin,represses
R3277 T5644 T5643 punct -,catenin
R3278 T5645 T5623 punct .,argues
R3279 T5647 T5648 advmod Rather,are
R3280 T5649 T5648 punct ", ",are
R3281 T5650 T5651 det the,findings
R3282 T5651 T5648 nsubj findings,are
R3283 T5652 T5648 acomp consistent,are
R3284 T5653 T5652 prep with,consistent
R3285 T5654 T5655 det a,report
R3286 T5655 T5653 pobj report,with
R3287 T5656 T5655 amod previous,report
R3288 T5657 T5658 mark that,required
R3289 T5658 T5655 acl required,report
R3290 T5659 T5660 compound E,cadherin
R3291 T5660 T5658 nsubjpass cadherin,required
R3292 T5661 T5660 punct -,cadherin
R3293 T5662 T5658 auxpass is,required
R3294 T5663 T5658 prep for,required
R3295 T5664 T5665 det the,translation
R3296 T5665 T5663 pobj translation,for
R3297 T5666 T5665 prep of,translation
R3298 T5667 T5668 compound α,catenin
R3299 T5668 T5670 compound catenin,mRNA
R3300 T5669 T5668 punct -,catenin
R3301 T5670 T5666 pobj mRNA,of
R3302 T5671 T5672 punct [,22
R3303 T5672 T5648 parataxis 22,are
R3304 T5673 T5672 punct ],22
R3305 T5674 T5648 punct .,are
R3306 T5676 T5677 prep Despite,displayed
R3307 T5678 T5679 det the,reductions
R3308 T5679 T5676 pobj reductions,Despite
R3309 T5680 T5679 prep in,reductions
R3310 T5681 T5682 compound AJ,markers
R3311 T5682 T5680 pobj markers,in
R3312 T5683 T5677 punct ", ",displayed
R3313 T5684 T5685 compound Tg,skin
R3314 T5685 T5677 nsubj skin,displayed
R3315 T5686 T5677 advmod still,displayed
R3316 T5687 T5688 amod sealed,membranes
R3317 T5688 T5677 dobj membranes,displayed
R3318 T5689 T5688 cc and,membranes
R3319 T5690 T5691 amod intercellular,junctions
R3320 T5691 T5688 conj junctions,membranes
R3321 T5692 T5693 dep that,were
R3322 T5693 T5691 relcl were,junctions
R3323 T5694 T5693 advmod largely,were
R3324 T5695 T5693 acomp intact,were
R3325 T5696 T5693 punct ", ",were
R3326 T5697 T5698 mark as,judged
R3327 T5698 T5693 advcl judged,were
R3328 T5699 T5698 prep by,judged
R3329 T5700 T5701 amod ultrastructural,analyses
R3330 T5701 T5699 pobj analyses,by
R3331 T5702 T5703 punct (,data
R3332 T5703 T5677 meta data,displayed
R3333 T5704 T5703 amod unpublished,data
R3334 T5705 T5703 punct ),data
R3335 T5706 T5677 punct .,displayed
R3336 T5708 T5709 prep In,resembled
R3337 T5710 T5711 det this,respect
R3338 T5711 T5708 pobj respect,In
R3339 T5712 T5709 punct ", ",resembled
R3340 T5713 T5714 det the,epithelium
R3341 T5714 T5709 nsubj epithelium,resembled
R3342 T5715 T5714 compound skin,epithelium
R3343 T5716 T5709 dobj that,resembled
R3344 T5717 T5716 prep of,that
R3345 T5718 T5719 det the,bud
R3346 T5719 T5717 pobj bud,of
R3347 T5720 T5719 compound hair,bud
R3348 T5721 T5719 punct ", ",bud
R3349 T5722 T5723 advmod where,is
R3350 T5723 T5719 relcl is,bud
R3351 T5724 T5725 det the,regulation
R3352 T5725 T5723 nsubj regulation,is
R3353 T5726 T5725 amod down,regulation
R3354 T5727 T5725 punct -,regulation
R3355 T5728 T5725 prep in,regulation
R3356 T5729 T5730 compound junction,proteins
R3357 T5730 T5728 pobj proteins,in
R3358 T5731 T5723 acomp permissive,is
R3359 T5732 T5723 prep for,is
R3360 T5733 T5734 compound cell,cell
R3361 T5734 T5736 compound cell,remodeling
R3362 T5735 T5734 punct -,cell
R3363 T5736 T5732 pobj remodeling,for
R3364 T5737 T5723 prep without,is
R3365 T5738 T5737 pcomp abrogating,without
R3366 T5739 T5740 amod intercellular,adhesion
R3367 T5740 T5738 dobj adhesion,abrogating
R3368 T5741 T5709 punct .,resembled
R3369 T5743 T5744 det The,similarities
R3370 T5744 T5745 nsubj similarities,extended
R3371 T5746 T5744 prep between,similarities
R3372 T5747 T5748 compound Snail,epidermis
R3373 T5748 T5746 pobj epidermis,between
R3374 T5749 T5748 compound Tg,epidermis
R3375 T5750 T5748 cc and,epidermis
R3376 T5751 T5752 compound hair,buds
R3377 T5752 T5748 conj buds,epidermis
R3378 T5753 T5745 prep to,extended
R3379 T5754 T5755 det the,state
R3380 T5755 T5753 pobj state,to
R3381 T5756 T5755 amod hyperproliferative,state
R3382 T5757 T5745 punct ", ",extended
R3383 T5758 T5745 advcl leading,extended
R3384 T5759 T5758 dobj us,leading
R3385 T5760 T5761 aux to,wonder
R3386 T5761 T5758 xcomp wonder,leading
R3387 T5762 T5763 mark whether,contribute
R3388 T5763 T5761 ccomp contribute,wonder
R3389 T5764 T5765 det the,regulation
R3390 T5765 T5763 nsubj regulation,contribute
R3391 T5766 T5765 amod down,regulation
R3392 T5767 T5765 punct -,regulation
R3393 T5768 T5765 prep of,regulation
R3394 T5769 T5770 compound AJ,proteins
R3395 T5770 T5768 pobj proteins,of
R3396 T5771 T5763 aux might,contribute
R3397 T5772 T5763 prep to,contribute
R3398 T5773 T5774 det this,condition
R3399 T5774 T5772 pobj condition,to
R3400 T5775 T5745 punct .,extended
R3401 T5777 T5778 prep Given,used
R3402 T5779 T5780 det the,increase
R3403 T5780 T5777 pobj increase,Given
R3404 T5781 T5780 prep in,increase
R3405 T5782 T5783 compound pMAPK,staining
R3406 T5783 T5781 pobj staining,in
R3407 T5784 T5780 prep in,increase
R3408 T5785 T5786 compound Snail,epidermis
R3409 T5786 T5784 pobj epidermis,in
R3410 T5787 T5786 compound Tg,epidermis
R3411 T5788 T5789 punct (,see
R3412 T5789 T5780 parataxis see,increase
R3413 T5790 T5791 compound Figure,2G
R3414 T5791 T5789 dobj 2G,see
R3415 T5792 T5789 punct ),see
R3416 T5793 T5778 punct ", ",used
R3417 T5794 T5778 nsubj we,used
R3418 T5795 T5796 compound pMAPK,levels
R3419 T5796 T5778 dobj levels,used
R3420 T5797 T5778 prep as,used
R3421 T5798 T5799 poss our,assay
R3422 T5799 T5797 pobj assay,as
R3423 T5800 T5801 aux to,test
R3424 T5801 T5778 advcl test,used
R3425 T5802 T5803 mark whether,contributed
R3426 T5803 T5801 ccomp contributed,test
R3427 T5804 T5805 det the,loss
R3428 T5805 T5803 nsubj loss,contributed
R3429 T5806 T5805 prep of,loss
R3430 T5807 T5808 compound E,cadherin
R3431 T5808 T5806 pobj cadherin,of
R3432 T5809 T5808 punct -,cadherin
R3433 T5810 T5803 prep to,contributed
R3434 T5811 T5812 det the,increase
R3435 T5812 T5810 pobj increase,to
R3436 T5813 T5814 npadvmod Snail,mediated
R3437 T5814 T5812 amod mediated,increase
R3438 T5815 T5814 punct -,mediated
R3439 T5816 T5812 prep in,increase
R3440 T5817 T5816 pobj proliferation,in
R3441 T5818 T5778 punct .,used
R3442 T5820 T5821 advcl Consistent,exhibited
R3443 T5822 T5820 prep with,Consistent
R3444 T5823 T5824 poss our,observations
R3445 T5824 T5822 pobj observations,with
R3446 T5825 T5826 advmod in,vivo
R3447 T5826 T5824 amod vivo,observations
R3448 T5827 T5821 punct ", ",exhibited
R3449 T5828 T5829 amod transfected,keratinocytes
R3450 T5829 T5821 nsubj keratinocytes,exhibited
R3451 T5830 T5829 acl expressing,keratinocytes
R3452 T5831 T5830 dobj Snail,expressing
R3453 T5832 T5833 det a,increase
R3454 T5833 T5821 dobj increase,exhibited
R3455 T5834 T5833 amod substantial,increase
R3456 T5835 T5833 prep in,increase
R3457 T5836 T5837 compound pMAPK,levels
R3458 T5837 T5835 pobj levels,in
R3459 T5838 T5833 amod relative,increase
R3460 T5839 T5838 prep to,relative
R3461 T5840 T5841 compound control,cells
R3462 T5841 T5839 pobj cells,to
R3463 T5842 T5843 punct (,4D
R3464 T5843 T5821 parataxis 4D,exhibited
R3465 T5844 T5843 compound Figure,4D
R3466 T5845 T5843 punct ),4D
R3467 T5846 T5821 punct .,exhibited
R3468 T5848 T5849 nsubj Coexpression,appeared
R3469 T5850 T5848 prep of,Coexpression
R3470 T5851 T5852 compound E,cadherin
R3471 T5852 T5850 pobj cadherin,of
R3472 T5853 T5852 punct -,cadherin
R3473 T5854 T5848 prep with,Coexpression
R3474 T5855 T5854 pobj Snail,with
R3475 T5856 T5857 aux to,abrogate
R3476 T5857 T5849 xcomp abrogate,appeared
R3477 T5858 T5859 det this,effect
R3478 T5859 T5857 dobj effect,abrogate
R3479 T5860 T5849 punct .,appeared
R3480 T5862 T5863 advmod Together,raised
R3481 T5864 T5863 punct ", ",raised
R3482 T5865 T5866 det these,findings
R3483 T5866 T5863 nsubj findings,raised
R3484 T5867 T5868 det the,possibility
R3485 T5868 T5863 dobj possibility,raised
R3486 T5869 T5870 mark that,participate
R3487 T5870 T5868 acl participate,possibility
R3488 T5871 T5872 det an,protein
R3489 T5872 T5870 nsubj protein,participate
R3490 T5873 T5874 npadvmod AJ,associated
R3491 T5874 T5872 amod associated,protein
R3492 T5875 T5874 punct -,associated
R3493 T5876 T5877 dep that,sequestered
R3494 T5877 T5872 relcl sequestered,protein
R3495 T5878 T5877 auxpass is,sequestered
R3496 T5879 T5877 advmod normally,sequestered
R3497 T5880 T5877 prep at,sequestered
R3498 T5881 T5882 det the,membrane
R3499 T5882 T5880 pobj membrane,at
R3500 T5883 T5882 compound plasma,membrane
R3501 T5884 T5870 aux may,participate
R3502 T5885 T5870 prep in,participate
R3503 T5886 T5887 det a,pathway
R3504 T5887 T5885 pobj pathway,in
R3505 T5888 T5887 compound proliferation,pathway
R3506 T5889 T5887 compound signaling,pathway
R3507 T5890 T5891 advmod when,deconstructed
R3508 T5891 T5870 advcl deconstructed,participate
R3509 T5892 T5891 nsubjpass AJs,deconstructed
R3510 T5893 T5891 auxpass are,deconstructed
R3511 T5894 T5863 punct .,raised
R3512 T5896 T5897 amod Numerous,studies
R3513 T5897 T5898 nsubj studies,correlated
R3514 T5899 T5898 aux have,correlated
R3515 T5900 T5901 det a,regulation
R3516 T5901 T5898 dobj regulation,correlated
R3517 T5902 T5901 amod down,regulation
R3518 T5903 T5901 punct -,regulation
R3519 T5904 T5901 prep of,regulation
R3520 T5905 T5906 compound E,cadherin
R3521 T5906 T5904 pobj cadherin,of
R3522 T5907 T5906 punct -,cadherin
R3523 T5908 T5898 prep with,correlated
R3524 T5909 T5910 det a,translocation
R3525 T5910 T5908 pobj translocation,with
R3526 T5911 T5910 prep of,translocation
R3527 T5912 T5913 compound β,catenin
R3528 T5913 T5911 pobj catenin,of
R3529 T5914 T5913 punct -,catenin
R3530 T5915 T5910 prep to,translocation
R3531 T5916 T5917 det the,nucleus
R3532 T5917 T5915 pobj nucleus,to
R3533 T5918 T5910 cc and,translocation
R3534 T5919 T5920 det a,transactivation
R3535 T5920 T5910 conj transactivation,translocation
R3536 T5921 T5920 prep of,transactivation
R3537 T5922 T5921 pobj genes,of
R3538 T5923 T5924 dep that,regulated
R3539 T5924 T5922 relcl regulated,genes
R3540 T5925 T5924 auxpass are,regulated
R3541 T5926 T5924 agent by,regulated
R3542 T5927 T5928 det the,family
R3543 T5928 T5926 pobj family,by
R3544 T5929 T5928 nmod LEF,family
R3545 T5930 T5929 punct -,LEF
R3546 T5931 T5929 nummod 1,LEF
R3547 T5932 T5929 punct /,LEF
R3548 T5933 T5934 compound T,cell
R3549 T5934 T5935 compound cell,factor
R3550 T5935 T5929 appos factor,LEF
R3551 T5936 T5935 punct (,factor
R3552 T5937 T5935 appos TCF,factor
R3553 T5938 T5928 punct ),family
R3554 T5939 T5928 prep of,family
R3555 T5940 T5941 npadvmod DNA,binding
R3556 T5941 T5942 amod binding,proteins
R3557 T5942 T5939 pobj proteins,of
R3558 T5943 T5944 punct [,25
R3559 T5944 T5898 parataxis 25,correlated
R3560 T5945 T5944 nummod 23,25
R3561 T5946 T5944 punct ",",25
R3562 T5947 T5944 nummod 24,25
R3563 T5948 T5944 punct ",",25
R3564 T5949 T5944 punct ],25
R3565 T5950 T5898 punct .,correlated
R3566 T5952 T5953 det The,presence
R3567 T5953 T5954 nsubj presence,was
R3568 T5955 T5953 prep of,presence
R3569 T5956 T5957 amod nuclear,D
R3570 T5957 T5955 pobj D,of
R3571 T5958 T5957 compound cyclin,D
R3572 T5959 T5953 prep in,presence
R3573 T5960 T5961 amod hyperproliferative,epidermis
R3574 T5961 T5959 pobj epidermis,in
R3575 T5962 T5961 compound Snail,epidermis
R3576 T5963 T5961 compound Tg,epidermis
R3577 T5964 T5965 advmod particularly,intriguing
R3578 T5965 T5954 acomp intriguing,was
R3579 T5966 T5967 mark since,reported
R3580 T5967 T5954 advcl reported,was
R3581 T5968 T5969 amod prior,studies
R3582 T5969 T5967 nsubj studies,reported
R3583 T5970 T5967 aux have,reported
R3584 T5971 T5972 compound cyclin,D
R3585 T5972 T5973 compound D,gene
R3586 T5973 T5967 dobj gene,reported
R3587 T5974 T5967 prep as,reported
R3588 T5975 T5976 det a,target
R3589 T5976 T5974 pobj target,as
R3590 T5977 T5976 amod direct,target
R3591 T5978 T5976 prep of,target
R3592 T5979 T5980 nmod TCF,β
R3593 T5980 T5982 nmod β,transcription
R3594 T5981 T5980 punct /,β
R3595 T5982 T5978 pobj transcription,of
R3596 T5983 T5980 punct -,β
R3597 T5984 T5980 appos catenin,β
R3598 T5985 T5986 punct [,26
R3599 T5986 T5954 parataxis 26,was
R3600 T5987 T5986 punct ],26
R3601 T5988 T5954 punct .,was
R3602 T5990 T5991 nsubj This,said
R3603 T5991 T5992 advcl said,detect
R3604 T5993 T5992 punct ", ",detect
R3605 T5994 T5992 nsubj we,detect
R3606 T5995 T5992 aux did,detect
R3607 T5996 T5992 neg not,detect
R3608 T5997 T5998 amod nuclear,catenin
R3609 T5998 T5992 dobj catenin,detect
R3610 T5999 T5998 compound β,catenin
R3611 T6000 T5998 punct -,catenin
R3612 T6001 T5992 prep in,detect
R3613 T6002 T6003 poss our,epidermis
R3614 T6003 T6001 pobj epidermis,in
R3615 T6004 T6003 compound Tg,epidermis
R3616 T6005 T5992 punct ", ",detect
R3617 T6006 T5992 cc and,detect
R3618 T6007 T6008 csubj mating,gave
R3619 T6008 T5992 conj gave,detect
R3620 T6009 T6010 det the,mice
R3621 T6010 T6007 dobj mice,mating
R3622 T6011 T6010 compound Snail,mice
R3623 T6012 T6010 compound Tg,mice
R3624 T6013 T6007 prep against,mating
R3625 T6014 T6015 det the,mouse
R3626 T6015 T6013 pobj mouse,against
R3627 T6016 T6015 compound TOPGal,mouse
R3628 T6017 T6015 compound reporter,mouse
R3629 T6018 T6019 punct [,20
R3630 T6019 T6007 parataxis 20,mating
R3631 T6020 T6019 punct ],20
R3632 T6021 T6022 det no,signs
R3633 T6022 T6008 dobj signs,gave
R3634 T6023 T6022 prep of,signs
R3635 T6024 T6025 amod ectopic,activity
R3636 T6025 T6023 pobj activity,of
R3637 T6026 T6025 nmod LEF,activity
R3638 T6027 T6026 punct -,LEF
R3639 T6028 T6026 nummod 1,LEF
R3640 T6029 T6026 punct /,LEF
R3641 T6030 T6026 appos Tcf,LEF
R3642 T6031 T6026 punct /,LEF
R3643 T6032 T6033 compound β,catenin
R3644 T6033 T6026 appos catenin,LEF
R3645 T6034 T6033 punct -,catenin
R3646 T6035 T6036 punct (,data
R3647 T6036 T6008 meta data,gave
R3648 T6037 T6036 amod unpublished,data
R3649 T6038 T6036 punct ),data
R3650 T6039 T5992 punct .,detect
R3651 T6041 T6042 nsubj We,turned
R3652 T6043 T6042 advmod next,turned
R3653 T6044 T6042 prep to,turned
R3654 T6045 T6046 det the,presence
R3655 T6093 T6091 nummod 2,protein
R3656 T6046 T6044 pobj presence,to
R3657 T6094 T6091 punct (,protein
R3658 T6095 T6091 appos Grb,protein
R3659 T6047 T6046 prep of,presence
R3660 T6096 T6095 punct -,Grb
R3661 T6097 T6095 nummod 2,Grb
R3662 T6048 T6049 amod cytoplasmic,Ajuba
R3663 T6098 T6091 punct ),protein
R3664 T6099 T6091 punct /,protein
R3665 T6100 T6091 appos son,protein
R3666 T6049 T6047 pobj Ajuba,of
R3667 T6101 T6100 prep of,son
R3668 T6102 T6101 pobj sevenless,of
R3669 T6103 T6100 punct (,son
R3670 T6050 T6042 prep for,turned
R3671 T6104 T6100 appos Sos,son
R3672 T6105 T6091 punct ),protein
R3673 T6106 T6091 punct ", ",protein
R3674 T6051 T6052 det a,link
R3675 T6107 T6108 det the,factor
R3676 T6108 T6091 appos factor,protein
R3677 T6109 T6108 compound nucleotide,factor
R3678 T6052 T6050 pobj link,for
R3679 T6110 T6108 compound exchange,factor
R3680 T6111 T6108 prep for,factor
R3681 T6112 T6111 pobj Ras,for
R3682 T6053 T6052 amod possible,link
R3683 T6113 T6112 punct ", ",Ras
R3684 T6114 T6115 dep which,is
R3685 T6115 T6112 relcl is,Ras
R3686 T6054 T6052 amod mechanistic,link
R3687 T6116 T6115 advmod upstream,is
R3688 T6117 T6116 prep from,upstream
R3689 T6118 T6117 pobj activation,from
R3690 T6119 T6118 prep of,activation
R3691 T6055 T6052 prep to,link
R3694 T6056 T6057 det the,increase
R3695 T6122 T6067 parataxis 9,associate
R3696 T6123 T6122 punct ],9
R3697 T6124 T6067 punct .,associate
R3698 T6057 T6055 pobj increase,to
R3699 T6126 T6127 prep Given,examined
R3700 T6058 T6057 amod proliferative,increase
R3701 T6128 T6129 det the,increase
R3702 T6129 T6126 pobj increase,Given
R3703 T6130 T6129 prep in,increase
R3704 T6131 T6132 compound pMAPK,staining
R3705 T6059 T6057 prep in,increase
R3706 T6132 T6130 pobj staining,in
R3707 T6060 T6061 poss our,epidermis
R3708 T6133 T6129 prep in,increase
R3709 T6134 T6135 compound Tg,skin
R3710 T6061 T6059 pobj epidermis,in
R3711 T6135 T6133 pobj skin,in
R3712 T6136 T6127 punct ", ",examined
R3713 T6137 T6127 nsubj we,examined
R3714 T6062 T6061 compound Snail,epidermis
R3715 T6138 T6139 det the,possibility
R3716 T6139 T6127 dobj possibility,examined
R3717 T6140 T6141 mark that,changed
R3718 T6063 T6061 compound Tg,epidermis
R3719 T6141 T6139 acl changed,possibility
R3720 T6142 T6141 nsubj Ajuba,changed
R3721 T6143 T6141 aux might,changed
R3722 T6064 T6042 punct .,turned
R3723 T6144 T6141 aux have,changed
R3724 T6145 T6146 poss its,partner
R3725 T6146 T6141 dobj partner,changed
R3726 T6066 T6067 prep In,associate
R3727 T6147 T6146 compound binding,partner
R3728 T6148 T6141 prep in,changed
R3729 T6149 T6150 npadvmod Snail,expressing
R3730 T6150 T6152 amod expressing,epidermis
R3731 T6151 T6150 punct -,expressing
R3732 T6068 T6066 pobj addition,In
R3733 T6152 T6148 pobj epidermis,in
R3734 T6153 T6127 punct .,examined
R3735 T6155 T6156 advmod Interestingly,detected
R3736 T6069 T6068 prep to,addition
R3737 T6157 T6156 punct ", ",detected
R3738 T6070 T6071 poss its,ability
R3739 T6158 T6156 nsubjpass Ajuba,detected
R3740 T6159 T6156 auxpass was,detected
R3741 T6160 T6156 advmod readily,detected
R3742 T6071 T6069 pobj ability,to
R3743 T6161 T6156 prep in,detected
R3744 T6162 T6163 amod anti-Grb,immunoprecipitates
R3745 T6163 T6161 pobj immunoprecipitates,in
R3746 T6072 T6071 amod documented,ability
R3747 T6164 T6162 punct -,anti-Grb
R3748 T6165 T6162 advmod 2,anti-Grb
R3749 T6073 T6074 aux to,bind
R3750 T6166 T6163 prep of,immunoprecipitates
R3751 T6167 T6168 compound protein,lysates
R3752 T6168 T6166 pobj lysates,of
R3753 T6074 T6071 acl bind,ability
R3754 T6169 T6168 prep from,lysates
R3755 T6170 T6169 pobj skins,from
R3756 T6171 T6170 prep of,skins
R3757 T6075 T6076 compound α,catenin
R3758 T6172 T6173 compound Snail,mice
R3759 T6173 T6171 pobj mice,of
R3760 T6076 T6074 dobj catenin,bind
R3761 T6174 T6173 compound Tg,mice
R3762 T6175 T6169 cc but,from
R3763 T6176 T6175 neg not,but
R3764 T6077 T6076 punct -,catenin
R3765 T6177 T6169 conj from,from
R3766 T6178 T6179 det the,animals
R3767 T6078 T6079 punct [,10
R3768 T6179 T6177 pobj animals,from
R3769 T6180 T6179 amod corresponding,animals
R3770 T6181 T6182 amod wild,type
R3771 T6079 T6068 parataxis 10,addition
R3772 T6182 T6179 nmod type,animals
R3773 T6183 T6182 punct -,type
R3774 T6184 T6182 punct (,type
R3775 T6080 T6079 punct ],10
R3776 T6185 T6182 appos WT,type
R3777 T6186 T6179 punct ),animals
R3778 T6081 T6067 punct ", ",associate
R3779 T6187 T6188 punct (,4E
R3780 T6188 T6156 parataxis 4E,detected
R3781 T6189 T6188 compound Figure,4E
R3782 T6082 T6067 nsubj Ajuba,associate
R3783 T6190 T6188 punct ),4E
R3784 T6191 T6156 punct .,detected
R3785 T6083 T6067 aux can,associate
R3786 T6193 T6194 advmod When,repeated
R3787 T6194 T6198 advcl repeated,detected
R3788 T6195 T6196 det these,experiments
R3789 T6084 T6067 advmod also,associate
R3790 T6196 T6194 nsubjpass experiments,repeated
R3791 T6197 T6194 auxpass were,repeated
R3792 T6085 T6067 prep with,associate
R3793 T6086 T6087 compound growth,factor
R3794 T6087 T6088 compound factor,receptor
R3795 T6199 T6194 prep with,repeated
R3796 T6088 T6089 npadvmod receptor,bound
R3797 T6200 T6201 compound α,catenin
R3798 T6201 T6203 npadvmod catenin,null
R3799 T6202 T6201 punct -,catenin
R3800 T6089 T6091 amod bound,protein
R3801 T6203 T6205 amod null,epidermis
R3802 T6204 T6203 punct -,null
R3803 T6205 T6199 pobj epidermis,with
R3804 T6090 T6089 punct -,bound
R3805 T6206 T6198 punct ", ",detected
R3806 T6207 T6208 det a,association
R3807 T6208 T6198 nsubjpass association,detected
R3808 T6209 T6208 amod similar,association
R3809 T6210 T6211 nmod Grb,Ajuba
R3810 T6211 T6208 compound Ajuba,association
R3811 T6091 T6085 pobj protein,with
R3812 T6212 T6211 punct -,Ajuba
R3813 T6213 T6211 nummod 2,Ajuba
R3814 T6214 T6211 punct -,Ajuba
R3815 T6215 T6198 auxpass was,detected
R3816 T6092 T6091 punct -,protein
R3817 T6216 T6198 punct ", ",detected
R3818 T6217 T6198 cc and,detected
R3819 T6218 T6219 advmod again,detected
R3820 T6303 T6286 acomp relevant,is
R3821 T6219 T6198 conj detected,detected
R3822 T6220 T6219 punct ", ",detected
R3823 T6221 T6222 det this,interaction
R3824 T6222 T6219 nsubjpass interaction,detected
R3825 T6223 T6219 auxpass was,detected
R3826 T6304 T6303 prep to,relevant
R3827 T6224 T6219 neg not,detected
R3828 T6225 T6219 prep in,detected
R3829 T6226 T6227 det the,extracts
R3830 T6227 T6225 pobj extracts,in
R3831 T6305 T6306 det the,state
R3832 T6228 T6227 compound protein,extracts
R3833 T6229 T6227 prep from,extracts
R3834 T6230 T6231 compound control,skin
R3835 T6306 T6304 pobj state,to
R3836 T6231 T6229 pobj skin,from
R3837 T6232 T6231 compound littermate,skin
R3838 T6233 T6234 punct (,4E
R3839 T6307 T6306 amod hyperproliferative,state
R3840 T6234 T6219 parataxis 4E,detected
R3841 T6235 T6234 compound Figure,4E
R3842 T6236 T6234 punct ),4E
R3843 T6308 T6306 prep of,state
R3844 T6237 T6198 punct .,detected
R3845 T6239 T6240 advmod Together,demonstrate
R3846 T6309 T6310 det a,keratinocyte
R3847 T6241 T6240 punct ", ",demonstrate
R3848 T6242 T6243 det these,data
R3849 T6243 T6240 nsubj data,demonstrate
R3850 T6310 T6308 pobj keratinocyte,of
R3851 T6244 T6245 mark that,allows
R3852 T6311 T6301 punct ", ",expected
R3853 T6245 T6240 ccomp allows,demonstrate
R3854 T6246 T6247 det the,reduction
R3855 T6312 T6301 advmod then,expected
R3856 T6247 T6245 nsubj reduction,allows
R3857 T6248 T6247 prep in,reduction
R3858 T6249 T6250 compound α,catenin
R3859 T6250 T6252 compound catenin,levels
R3860 T6251 T6250 punct -,catenin
R3861 T6252 T6248 pobj levels,in
R3862 T6253 T6247 punct ", ",reduction
R3863 T6313 T6301 nsubjpass overexpression,expected
R3864 T6254 T6255 preconj either,by
R3865 T6255 T6247 prep by,reduction
R3866 T6256 T6257 npadvmod Snail,mediated
R3867 T6314 T6313 prep of,overexpression
R3868 T6257 T6259 amod mediated,regulation
R3869 T6258 T6257 punct -,mediated
R3870 T6259 T6255 pobj regulation,by
R3871 T6315 T6314 pobj Ajuba,of
R3872 T6260 T6259 amod down,regulation
R3873 T6261 T6259 punct -,regulation
R3874 T6262 T6259 prep of,regulation
R3875 T6316 T6301 aux would,expected
R3876 T6263 T6264 compound E,cadherin
R3877 T6264 T6262 pobj cadherin,of
R3878 T6265 T6264 punct -,cadherin
R3880 T6266 T6255 cc or,by
R3881 T6267 T6255 conj by,by
R3882 T6268 T6269 compound α,catenin
R3883 T6318 T6319 aux to,bypass
R3884 T6269 T6271 npadvmod catenin,conditional
R3885 T6270 T6269 punct -,catenin
R3886 T6271 T6272 amod conditional,targeting
R3887 T6319 T6301 xcomp bypass,expected
R3888 T6272 T6267 pobj targeting,by
R3889 T6273 T6245 punct ", ",allows
R3890 T6274 T6275 nsubj Ajuba,interact
R3891 T6320 T6321 det the,competition
R3892 T6275 T6245 ccomp interact,allows
R3893 T6276 T6275 aux to,interact
R3894 T6277 T6275 prep with,interact
R3895 T6321 T6319 dobj competition,bypass
R3896 T6278 T6277 pobj Grb,with
R3897 T6279 T6278 punct -,Grb
R3898 T6280 T6278 nummod 2,Grb
R3899 T6281 T6278 punct /,Grb
R3900 T6322 T6319 cc and,bypass
R3901 T6282 T6278 appos Sos,Grb
R3902 T6283 T6240 punct .,demonstrate
R3903 T6323 T6319 conj promote,bypass
R3904 T6285 T6286 mark If,is
R3905 T6286 T6301 advcl is,expected
R3906 T6287 T6288 det the,competition
R3907 T6324 T6323 dobj activation,promote
R3908 T6288 T6286 nsubj competition,is
R3909 T6289 T6288 prep between,competition
R3910 T6290 T6289 pobj Grb,between
R3911 T6291 T6290 punct -,Grb
R3912 T6292 T6290 nummod 2,Grb
R3913 T6293 T6290 punct /,Grb
R3914 T6294 T6290 appos Sos,Grb
R3915 T6325 T6324 prep of,activation
R3916 T6295 T6290 cc and,Grb
R3917 T6296 T6297 compound α,catenin
R3918 T6297 T6290 conj catenin,Grb
R3919 T6326 T6327 det the,pathway
R3920 T6298 T6297 punct -,catenin
R3921 T6299 T6288 prep for,competition
R3922 T6300 T6299 pobj Ajuba,for
R3923 T6327 T6325 pobj pathway,of
R3924 T6302 T6303 advmod functionally,relevant
R3925 T6328 T6329 compound Ras,MAPK
R3926 T6329 T6327 compound MAPK,pathway
R3927 T6330 T6329 punct -,MAPK
R3928 T6331 T6324 prep in,activation
R3929 T6409 T6407 oprd inh,marked
R3930 T6410 T6405 punct ”,see
R3931 T6332 T6333 compound WT,keratinocytes
R3932 T6411 T6402 punct ),4F
R3933 T6412 T6413 punct [,27
R3934 T6413 T6380 parataxis 27,abolished
R3935 T6333 T6331 pobj keratinocytes,in
R3936 T6414 T6413 punct ],27
R3937 T6415 T6380 punct .,abolished
R3938 T6334 T6301 punct .,expected
R3939 T6417 T6418 poss Ajuba,domain
R3940 T6418 T6421 nsubj domain,is
R3941 T6419 T6417 case 's,Ajuba
R3942 T6336 T6337 advmod Indeed,were
R3943 T6420 T6418 amod pre-LIM,domain
R3944 T6422 T6423 det the,segment
R3945 T6423 T6421 attr segment,is
R3946 T6338 T6337 punct ", ",were
R3947 T6424 T6425 dep that,associates
R3948 T6339 T6340 advmod when,transfected
R3949 T6425 T6423 relcl associates,segment
R3950 T6426 T6425 prep with,associates
R3951 T6340 T6337 advcl transfected,were
R3952 T6427 T6428 poss Grb,domain
R3953 T6428 T6426 pobj domain,with
R3954 T6429 T6427 punct -,Grb
R3955 T6341 T6342 npadvmod serum,starved
R3956 T6430 T6427 nummod 2,Grb
R3957 T6431 T6427 case 's,Grb
R3958 T6432 T6433 nmod Src,homology
R3959 T6433 T6428 nmod homology,domain
R3960 T6342 T6344 amod starved,keratinocytes
R3961 T6434 T6433 punct -,homology
R3962 T6435 T6433 nummod 3,homology
R3963 T6436 T6437 punct [,9
R3964 T6437 T6421 parataxis 9,is
R3965 T6438 T6437 punct ],9
R3966 T6439 T6421 punct .,is
R3967 T6343 T6342 punct -,starved
R3968 T6441 T6442 advmod When,overexpressed
R3969 T6442 T6446 advcl overexpressed,observed
R3970 T6344 T6340 nsubjpass keratinocytes,transfected
R3971 T6443 T6444 det this,domain
R3972 T6444 T6442 nsubjpass domain,overexpressed
R3973 T6445 T6442 auxpass was,overexpressed
R3974 T6345 T6340 auxpass were,transfected
R3975 T6447 T6442 prep in,overexpressed
R3976 T6448 T6449 npadvmod serum,starved
R3977 T6346 T6340 advmod transiently,transfected
R3978 T6449 T6451 amod starved,keratinocytes
R3979 T6450 T6449 punct -,starved
R3980 T6451 T6447 pobj keratinocytes,in
R3981 T6347 T6340 prep with,transfected
R3982 T6452 T6446 punct ", ",observed
R3983 T6453 T6454 det a,elevation
R3984 T6454 T6446 nsubjpass elevation,observed
R3985 T6348 T6349 det an,vector
R3986 T6455 T6454 amod comparable,elevation
R3987 T6456 T6454 prep in,elevation
R3988 T6457 T6456 pobj pMAPK,in
R3989 T6458 T6446 auxpass was,observed
R3990 T6349 T6347 pobj vector,with
R3991 T6459 T6460 punct (,4F
R3992 T6460 T6446 parataxis 4F,observed
R3993 T6461 T6460 compound Figure,4F
R3994 T6350 T6351 compound Ajuba,expression
R3995 T6462 T6460 punct ),4F
R3996 T6463 T6446 punct .,observed
R3997 T6351 T6349 compound expression,vector
R3998 T6465 T6466 mark As,expected
R3999 T6466 T6467 advcl expected,blocked
R4000 T6352 T6337 punct ", ",were
R4001 T6468 T6467 punct ", ",blocked
R4002 T6469 T6470 det the,inhibitor
R4003 T6470 T6467 nsubj inhibitor,blocked
R4004 T6471 T6470 amod small,inhibitor
R4005 T6353 T6354 det the,levels
R4006 T6472 T6470 compound peptide,inhibitor
R4007 T6473 T6474 dep that,interrupts
R4008 T6474 T6470 relcl interrupts,inhibitor
R4009 T6354 T6337 nsubj levels,were
R4010 T6475 T6476 det the,association
R4011 T6476 T6474 dobj association,interrupts
R4012 T6477 T6476 nmod Grb,association
R4013 T6478 T6477 punct -,Grb
R4014 T6479 T6477 nummod 2,Grb
R4015 T6355 T6354 prep of,levels
R4016 T6480 T6477 punct /,Grb
R4017 T6481 T6477 appos Sos,Grb
R4018 T6482 T6483 det the,effects
R4019 T6483 T6467 dobj effects,blocked
R4020 T6356 T6355 pobj pMAPK,of
R4021 T6484 T6467 punct .,blocked
R4022 T6486 T6487 det These,data
R4023 T6357 T6358 preconj not,elevated
R4024 T6487 T6488 nsubj data,suggested
R4025 T6489 T6490 mark that,associate
R4026 T6358 T6337 acomp elevated,were
R4027 T6490 T6488 ccomp associate,suggested
R4028 T6491 T6490 prep by,associate
R4029 T6492 T6491 pcomp elevating,by
R4030 T6493 T6494 amod cytosolic,levels
R4031 T6359 T6357 advmod only,not
R4032 T6494 T6492 dobj levels,elevating
R4033 T6495 T6494 compound Ajuba,levels
R4034 T6496 T6490 punct ", ",associate
R4035 T6360 T6358 cc but,elevated
R4036 T6497 T6498 poss Ajuba,domain
R4037 T6498 T6490 nsubj domain,associate
R4038 T6499 T6497 case 's,Ajuba
R4039 T6500 T6498 amod pre-LIM,domain
R4040 T6361 T6360 advmod also,but
R4041 T6501 T6490 aux may,associate
R4042 T6502 T6490 prep with,associate
R4043 T6503 T6502 pobj Grb,with
R4044 T6362 T6358 conj comparable,elevated
R4045 T6504 T6503 punct -,Grb
R4046 T6505 T6503 nummod 2,Grb
R4047 T6506 T6503 punct /,Grb
R4048 T6363 T6362 prep to,comparable
R4049 T6507 T6503 appos Sos,Grb
R4050 T6508 T6490 prep in,associate
R4051 T6509 T6510 det a,manner
R4052 T6364 T6363 pobj those,to
R4053 T6510 T6508 pobj manner,in
R4054 T6511 T6512 dep that,stimulates
R4055 T6365 T6364 acl transfected,those
R4056 T6512 T6510 relcl stimulates,manner
R4057 T6513 T6514 poss its,activity
R4058 T6366 T6365 prep with,transfected
R4059 T6367 T6368 det the,transgene
R4060 T6368 T6366 pobj transgene,with
R4061 T6514 T6512 dobj activity,stimulates
R4062 T6515 T6514 compound nucleotide,activity
R4063 T6516 T6514 compound exchange,activity
R4064 T6369 T6370 compound K14,HASnail
R4065 T6517 T6512 cc and,stimulates
R4066 T6518 T6512 conj leads,stimulates
R4067 T6370 T6368 compound HASnail,transgene
R4068 T6519 T6518 prep to,leads
R4069 T6520 T6519 pobj activation,to
R4070 T6521 T6520 prep of,activation
R4071 T6371 T6370 punct -,HASnail
R4073 T6523 T6521 pobj pathway,of
R4074 T6524 T6525 compound Ras,MAPK
R4075 T6372 T6373 punct (,4F
R4076 T6525 T6523 compound MAPK,pathway
R4077 T6526 T6525 punct -,MAPK
R4078 T6527 T6488 punct .,suggested
R4079 T6373 T6337 parataxis 4F,were
R4080 T6529 T6530 mark Although,provides
R4082 T6374 T6373 compound Figure,4F
R4083 T6531 T6532 det this,pathway
R4084 T6532 T6530 nsubj pathway,provides
R4085 T6375 T6373 punct ),4F
R4086 T6534 T6535 nummod one,mechanism
R4087 T6535 T6530 dobj mechanism,provides
R4088 T6536 T6537 prep by,coupled
R4089 T6537 T6535 relcl coupled,mechanism
R4090 T6376 T6337 punct .,were
R4091 T6538 T6536 pobj which,by
R4092 T6539 T6540 compound Snail,expression
R4093 T6540 T6537 nsubjpass expression,coupled
R4094 T6378 T6379 det This,activation
R4095 T6541 T6540 cc and,expression
R4096 T6542 T6540 conj proliferation,expression
R4097 T6543 T6537 aux may,coupled
R4098 T6544 T6537 auxpass be,coupled
R4099 T6379 T6380 nsubjpass activation,abolished
R4100 T6545 T6537 prep in,coupled
R4101 T6546 T6547 compound skin,epithelium
R4102 T6547 T6545 pobj epithelium,in
R4103 T6381 T6380 auxpass was,abolished
R4104 T6548 T6533 punct ", ",known
R4105 T6549 T6550 amod proliferative,circuitries
R4106 T6550 T6533 nsubjpass circuitries,known
R4107 T6551 T6550 acl involving,circuitries
R4108 T6382 T6383 advmod when,treated
R4109 T6552 T6551 dobj AJs,involving
R4110 T6553 T6533 auxpass are,known
R4111 T6554 T6555 aux to,be
R4112 T6555 T6533 xcomp be,known
R4113 T6383 T6380 advcl treated,abolished
R4114 T6556 T6555 acomp complex,be
R4115 T6557 T6556 cc and,complex
R4116 T6558 T6559 advmod often,interwoven
R4117 T6559 T6556 conj interwoven,complex
R4118 T6560 T6533 punct .,known
R4119 T6384 T6383 nsubjpass cells,treated
R4120 T6562 T6563 amod Future,studies
R4121 T6563 T6564 nsubjpass studies,needed
R4122 T6385 T6383 auxpass were,treated
R4123 T6565 T6564 aux will,needed
R4124 T6566 T6564 auxpass be,needed
R4125 T6567 T6568 aux to,dissect
R4126 T6386 T6383 prep with,treated
R4127 T6568 T6564 advcl dissect,needed
R4128 T6569 T6568 advmod systematically,dissect
R4129 T6570 T6571 det these,intricacies
R4130 T6387 T6388 det a,inhibitor
R4131 T6571 T6568 dobj intricacies,dissect
R4132 T6572 T6571 amod putative,intricacies
R4133 T6573 T6568 prep at,dissect
R4134 T6388 T6386 pobj inhibitor,with
R4135 T6574 T6575 det a,level
R4136 T6575 T6573 pobj level,at
R4137 T6576 T6575 amod molecular,level
R4138 T6389 T6388 amod small,inhibitor
R4139 T6577 T6564 punct .,needed
R4140 T6390 T6388 compound peptide,inhibitor
R4141 T6391 T6392 dep that,interrupts
R4142 T6392 T6388 relcl interrupts,inhibitor
R4143 T6393 T6392 advmod specifically,interrupts
R4144 T6394 T6395 det the,interaction
R4145 T6395 T6392 dobj interaction,interrupts
R4146 T6396 T6395 nmod Grb,interaction
R4147 T6397 T6396 punct -,Grb
R4148 T6398 T6396 nummod 2,Grb
R4149 T6399 T6396 punct /,Grb
R4150 T6400 T6396 appos Sos,Grb
R4151 T6401 T6402 punct (,4F
R4152 T6402 T6380 parataxis 4F,abolished
R4153 T6403 T6402 compound Figure,4F
R4154 T6404 T6402 punct ;,4F
R4155 T6405 T6402 advcl see,4F
R4156 T6406 T6405 dobj lanes,see
R4157 T6407 T6406 acl marked,lanes
R4158 T6408 T6409 punct “,inh
R4172 T7354 T7355 csubj Probing,Reveals
R4173 T7356 T7357 det the,Regulation
R4174 T7357 T7354 dobj Regulation,Probing
R4175 T7358 T7357 prep of,Regulation
R4176 T7359 T7360 compound Snail,Gene
R4177 T7360 T7361 compound Gene,Expression
R4178 T7361 T7358 pobj Expression,of
R4179 T7362 T7363 det an,Link
R4180 T7363 T7355 dobj Link,Reveals
R4181 T7364 T7363 amod Essential,Link
R4182 T7365 T7363 prep to,Link
R4183 T7366 T7367 compound TGF,β2
R4184 T7367 T7369 compound β2,Signaling
R4185 T7368 T7367 punct -,β2
R4186 T7369 T7365 pobj Signaling,to
R4187 T7370 T7355 prep in,Reveals
R4188 T7371 T7372 det the,Bud
R4189 T7372 T7370 pobj Bud,in
R4190 T7373 T7372 amod Developing,Bud
R4191 T7374 T7372 compound Hair,Bud
R4192 T7376 T7377 det The,spike
R4193 T7377 T7379 nsubj spike,prompted
R4194 T7378 T7377 amod temporal,spike
R4195 T7380 T7377 prep of,spike
R4196 T7381 T7382 compound Snail,expression
R4197 T7382 T7380 pobj expression,of
R4198 T7383 T7382 compound mRNA,expression
R4199 T7384 T7377 prep in,spike
R4200 T7385 T7386 det the,bud
R4201 T7386 T7384 pobj bud,in
R4202 T7387 T7386 compound hair,bud
R4203 T7388 T7379 dobj us,prompted
R4204 T7389 T7390 aux to,consider
R4205 T7390 T7379 xcomp consider,prompted
R4206 T7391 T7392 det what,factor
R4207 T7392 T7393 dep factor,regulating
R4208 T7393 T7390 ccomp regulating,consider
R4209 T7394 T7392 punct (,factor
R4210 T7395 T7392 nmod s,factor
R4211 T7396 T7392 punct ),factor
R4212 T7397 T7393 aux may,regulating
R4213 T7398 T7393 aux be,regulating
R4214 T7399 T7400 det the,gene
R4215 T7400 T7393 dobj gene,regulating
R4216 T7401 T7400 compound Snail,gene
R4217 T7402 T7379 punct .,prompted
R4218 T7404 T7405 det A,variety
R4219 T7405 T7406 nsubj variety,have
R4220 T7407 T7405 prep of,variety
R4221 T7408 T7409 amod extracellular,signals
R4222 T7409 T7407 pobj signals,of
R4223 T7410 T7411 det an,impact
R4224 T7411 T7406 dobj impact,have
R4225 T7412 T7411 prep on,impact
R4226 T7413 T7414 det the,expression
R4227 T7414 T7412 pobj expression,on
R4228 T7415 T7416 compound cell,type
R4229 T7416 T7417 npadvmod type,specific
R4230 T7417 T7414 amod specific,expression
R4231 T7418 T7417 punct -,specific
R4232 T7419 T7414 prep of,expression
R4233 T7420 T7421 amod different,members
R4234 T7421 T7419 pobj members,of
R4235 T7422 T7423 compound Snail,family
R4236 T7423 T7421 compound family,members
R4237 T7424 T7406 punct ", ",have
R4238 T7425 T7406 cc and,have
R4239 T7426 T7427 nsubj many,affect
R4240 T7427 T7406 conj affect,have
R4241 T7428 T7426 prep of,many
R4242 T7429 T7428 pobj them,of
R4243 T7430 T7426 punct ", ",many
R4244 T7431 T7426 prep including,many
R4245 T7432 T7431 pobj Wnts,including
R4246 T7433 T7432 punct ", ",Wnts
R4247 T7434 T7432 conj BMPs,Wnts
R4248 T7435 T7434 punct ", ",BMPs
R4249 T7436 T7434 conj FGFs,BMPs
R4250 T7437 T7436 punct ", ",FGFs
R4251 T7438 T7436 cc and,FGFs
R4252 T7439 T7440 compound TGF,βs
R4253 T7440 T7436 conj βs,FGFs
R4254 T7441 T7440 punct -,βs
R4255 T7442 T7427 punct ", ",affect
R4256 T7443 T7427 advmod also,affect
R4257 T7444 T7445 compound hair,bud
R4258 T7445 T7446 compound bud,development
R4259 T7446 T7427 dobj development,affect
R4260 T7447 T7448 punct [,28
R4261 T7448 T7427 parataxis 28,affect
R4262 T7449 T7448 nummod 2,28
R4263 T7450 T7448 punct ",",28
R4264 T7451 T7448 nummod 16,28
R4265 T7452 T7448 punct ",",28
R4266 T7453 T7448 punct ],28
R4267 T7454 T7427 punct .,affect
R4268 T7456 T7457 mark Since,expressed
R4269 T7457 T7461 advcl expressed,focused
R4270 T7458 T7457 nsubjpass Snail,expressed
R4271 T7459 T7457 auxpass is,expressed
R4272 T7460 T7457 neg not,expressed
R4273 T7462 T7457 prep in,expressed
R4274 T7463 T7464 amod cultured,keratinocytes
R4275 T7464 T7462 pobj keratinocytes,in
R4276 T7465 T7464 compound skin,keratinocytes
R4277 T7466 T7467 dep that,secrete
R4278 T7467 T7464 relcl secrete,keratinocytes
R4279 T7468 T7469 amod active,BMPs
R4280 T7469 T7467 dobj BMPs,secrete
R4281 T7470 T7469 cc and,BMPs
R4282 T7471 T7469 conj FGFs,BMPs
R4283 T7472 T7473 punct (,see
R4284 T7473 T7457 parataxis see,expressed
R4285 T7474 T7475 compound Figure,1B
R4286 T7475 T7473 dobj 1B,see
R4287 T7476 T7473 punct ),see
R4288 T7477 T7461 punct ", ",focused
R4289 T7478 T7461 nsubj we,focused
R4290 T7479 T7480 poss our,attention
R4291 T7480 T7461 dobj attention,focused
R4292 T7481 T7461 prep on,focused
R4293 T7482 T7483 nmod Wnt,signaling
R4294 T7483 T7481 pobj signaling,on
R4295 T7484 T7482 cc and,Wnt
R4296 T7485 T7486 compound TGF,β
R4297 T7486 T7482 conj β,Wnt
R4298 T7487 T7486 punct -,β
R4299 T7488 T7483 prep as,signaling
R4300 T7489 T7490 advmod more,likely
R4301 T7490 T7491 amod likely,candidates
R4302 T7491 T7488 pobj candidates,as
R4303 T7492 T7491 prep for,candidates
R4304 T7493 T7494 compound Snail,induction
R4305 T7494 T7492 pobj induction,for
R4306 T7495 T7491 prep in,candidates
R4307 T7496 T7497 det this,type
R4308 T7497 T7495 pobj type,in
R4309 T7498 T7497 compound cell,type
R4310 T7499 T7461 punct .,focused
R4311 T7501 T7502 advmod Previously,showed
R4312 T7503 T7502 punct ", ",showed
R4313 T7504 T7502 nsubj we,showed
R4314 T7505 T7506 mark that,achieved
R4315 T7506 T7502 ccomp achieved,showed
R4316 T7507 T7508 amod effective,transmission
R4317 T7508 T7506 nsubjpass transmission,achieved
R4318 T7509 T7508 prep of,transmission
R4319 T7510 T7511 det a,signal
R4320 T7511 T7509 pobj signal,of
R4321 T7512 T7511 nmod Wnt,signal
R4322 T7513 T7512 punct -,Wnt
R4323 T7514 T7512 nummod 3a,Wnt
R4324 T7515 T7508 prep in,transmission
R4325 T7516 T7517 amod cultured,keratinocytes
R4326 T7517 T7515 pobj keratinocytes,in
R4327 T7518 T7506 aux can,achieved
R4328 T7519 T7506 auxpass be,achieved
R4329 T7520 T7506 prep through,achieved
R4330 T7521 T7522 poss their,exposure
R4331 T7522 T7520 pobj exposure,through
R4332 T7523 T7522 prep to,exposure
R4333 T7524 T7525 det the,inhibitor
R4334 T7525 T7523 pobj inhibitor,to
R4335 T7526 T7525 compound BMP,inhibitor
R4336 T7527 T7525 appos noggin,inhibitor
R4337 T7528 T7525 punct ", ",inhibitor
R4338 T7529 T7530 dep which,induces
R4339 T7530 T7525 relcl induces,inhibitor
R4340 T7531 T7532 nmod LEF,expression
R4341 T7532 T7530 dobj expression,induces
R4342 T7533 T7531 punct -,LEF
R4343 T7534 T7531 nummod 1,LEF
R4344 T7535 T7536 punct [,4
R4345 T7536 T7502 parataxis 4,showed
R4346 T7537 T7536 punct ],4
R4347 T7538 T7502 punct .,showed
R4348 T7540 T7541 advmod In,vitro
R4349 T7541 T7542 advmod vitro,regulated
R4350 T7543 T7542 punct ", ",regulated
R4351 T7544 T7545 det these,conditions
R4352 T7545 T7542 nsubj conditions,regulated
R4353 T7546 T7542 advmod down,regulated
R4354 T7547 T7542 punct -,regulated
R4355 T7548 T7549 det the,promoter
R4356 T7549 T7542 dobj promoter,regulated
R4357 T7550 T7551 compound E,cadherin
R4358 T7551 T7549 compound cadherin,promoter
R4359 T7552 T7551 punct -,cadherin
R4360 T7553 T7542 cc and,regulated
R4361 T7554 T7542 conj induced,regulated
R4362 T7555 T7556 det a,gene
R4363 T7556 T7554 dobj gene,induced
R4364 T7557 T7558 npadvmod LEF,sensitive
R4365 T7558 T7556 amod sensitive,gene
R4366 T7559 T7557 punct -,LEF
R4367 T7560 T7557 nummod 1,LEF
R4368 T7561 T7557 punct /,LEF
R4369 T7562 T7563 compound β,catenin
R4370 T7563 T7557 appos catenin,LEF
R4371 T7564 T7563 punct -,catenin
R4372 T7565 T7558 punct -,sensitive
R4373 T7566 T7556 compound reporter,gene
R4374 T7567 T7556 punct ", ",gene
R4375 T7568 T7556 appos TOPFLASH,gene
R4376 T7569 T7570 punct [,4
R4377 T7570 T7554 parataxis 4,induced
R4378 T7571 T7570 punct ],4
R4379 T7572 T7542 punct .,regulated
R4380 T7574 T7575 prep In,induced
R4381 T7576 T7574 pobj contrast,In
R4382 T7577 T7575 punct ", ",induced
R4383 T7578 T7579 compound Snail,expression
R4384 T7579 T7575 nsubjpass expression,induced
R4385 T7580 T7575 auxpass was,induced
R4386 T7581 T7575 neg not,induced
R4387 T7582 T7575 agent by,induced
R4388 T7583 T7584 det these,conditions
R4389 T7584 T7582 pobj conditions,by
R4390 T7585 T7586 punct (,5A
R4391 T7586 T7575 parataxis 5A,induced
R4392 T7587 T7586 compound Figure,5A
R4393 T7588 T7586 punct ),5A
R4394 T7589 T7575 punct .,induced
R4395 T7591 T7592 advmod Thus,support
R4396 T7593 T7592 punct ", ",support
R4397 T7594 T7592 prep despite,support
R4398 T7595 T7596 amod essential,roles
R4399 T7596 T7594 pobj roles,despite
R4400 T7597 T7596 prep for,roles
R4401 T7598 T7597 pobj Wnts,for
R4402 T7599 T7598 cc and,Wnts
R4403 T7600 T7598 conj noggin,Wnts
R4404 T7601 T7596 prep in,roles
R4405 T7602 T7603 compound hair,follicle
R4406 T7603 T7604 compound follicle,specification
R4407 T7604 T7601 pobj specification,in
R4408 T7605 T7606 punct [,30
R4409 T7606 T7596 parataxis 30,roles
R4410 T7607 T7606 nummod 4,30
R4411 T7608 T7606 punct ",",30
R4412 T7609 T7606 nummod 29,30
R4413 T7610 T7606 punct ",",30
R4414 T7611 T7606 punct ],30
R4415 T7612 T7592 punct ", ",support
R4416 T7613 T7614 poss our,studies
R4417 T7614 T7592 nsubj studies,support
R4418 T7615 T7592 aux did,support
R4419 T7616 T7592 neg not,support
R4420 T7617 T7618 det an,role
R4421 T7618 T7592 dobj role,support
R4422 T7619 T7618 amod essential,role
R4423 T7620 T7618 prep for,role
R4424 T7621 T7622 det these,signals
R4425 T7622 T7620 pobj signals,for
R4426 T7623 T7618 prep in,role
R4427 T7624 T7623 pcomp governing,in
R4428 T7625 T7626 compound Snail,expression
R4429 T7626 T7624 dobj expression,governing
R4430 T7627 T7624 prep in,governing
R4431 T7628 T7627 pobj keratinocytes,in
R4432 T7629 T7592 punct .,support
R4433 T7631 T7632 compound TGF,β1
R4434 T7632 T7634 nsubjpass β1,shown
R4435 T7633 T7632 punct -,β1
R4436 T7635 T7634 aux has,shown
R4437 T7636 T7634 auxpass been,shown
R4438 T7637 T7638 aux to,induce
R4439 T7638 T7634 xcomp induce,shown
R4440 T7639 T7640 compound Snail,members
R4441 T7640 T7638 dobj members,induce
R4442 T7641 T7640 compound family,members
R4443 T7642 T7638 prep in,induce
R4444 T7643 T7642 pobj hepatocytes,in
R4445 T7644 T7643 cc and,hepatocytes
R4446 T7645 T7643 conj heart,hepatocytes
R4447 T7646 T7647 punct [,31
R4448 T7647 T7634 parataxis 31,shown
R4449 T7648 T7647 nummod 15,31
R4450 T7649 T7647 punct ", ",31
R4451 T7650 T7647 punct ],31
R4452 T7651 T7634 punct .,shown
R4453 T7653 T7654 prep In,inhibits
R4454 T7655 T7653 pobj keratinocytes,In
R4455 T7656 T7654 punct ", ",inhibits
R4456 T7657 T7654 advmod however,inhibits
R4457 T7658 T7654 punct ", ",inhibits
R4458 T7659 T7660 compound TGF,β1
R4459 T7660 T7654 nsubj β1,inhibits
R4460 T7661 T7660 punct -,β1
R4461 T7662 T7663 compound keratinocyte,growth
R4462 T7663 T7654 dobj growth,inhibits
R4463 T7664 T7654 cc and,inhibits
R4464 T7665 T7654 conj seems,inhibits
R4465 T7666 T7667 aux to,involved
R4466 T7667 T7665 xcomp involved,seems
R4467 T7668 T7667 auxpass be,involved
R4468 T7669 T7667 prep in,involved
R4469 T7670 T7669 pcomp triggering,in
R4470 T7671 T7672 det the,phase
R4471 T7672 T7670 dobj phase,triggering
R4472 T7673 T7672 amod destructive,phase
R4473 T7674 T7672 prep of,phase
R4474 T7675 T7676 det the,follicle
R4475 T7676 T7674 pobj follicle,of
R4476 T7677 T7676 amod cycling,follicle
R4477 T7678 T7676 compound hair,follicle
R4478 T7679 T7680 punct [,32
R4479 T7680 T7665 parataxis 32,seems
R4480 T7681 T7680 punct ],32
R4481 T7682 T7654 punct .,inhibits
R4482 T7684 T7685 prep Of,blocked
R4483 T7686 T7687 det the,mutations
R4484 T7687 T7684 pobj mutations,Of
R4485 T7688 T7687 nmod loss,mutations
R4486 T7689 T7688 punct -,loss
R4487 T7690 T7688 prep of,loss
R4488 T7691 T7690 punct -,of
R4489 T7692 T7690 pobj function,of
R4490 T7693 T7687 acl generated,mutations
R4491 T7694 T7693 prep in,generated
R4492 T7695 T7694 pobj each,in
R4493 T7696 T7695 prep of,each
R4494 T7697 T7698 det the,genes
R4495 T7698 T7696 pobj genes,of
R4496 T7699 T7700 compound TGF,β
R4497 T7700 T7698 compound β,genes
R4498 T7701 T7700 punct -,β
R4499 T7702 T7685 punct ", ",blocked
R4500 T7703 T7704 advmod only,state
R4501 T7704 T7685 nsubj state,blocked
R4502 T7705 T7704 det the,state
R4503 T7706 T7707 nmod TGF,β2
R4504 T7707 T7704 nmod β2,state
R4505 T7708 T7707 punct -,β2
R4506 T7709 T7704 amod null,state
R4507 T7710 T7711 compound follicle,development
R4508 T7711 T7685 dobj development,blocked
R4509 T7712 T7685 prep at,blocked
R4510 T7713 T7714 det the,stage
R4511 T7714 T7712 pobj stage,at
R4512 T7715 T7716 compound hair,bud
R4513 T7716 T7714 compound bud,stage
R4514 T7717 T7718 punct [,32
R4515 T7718 T7685 parataxis 32,blocked
R4516 T7719 T7718 punct ],32
R4517 T7720 T7685 punct .,blocked
R4518 T7722 T7723 advmod Thus,turned
R4519 T7724 T7723 punct ", ",turned
R4520 T7725 T7723 nsubj we,turned
R4521 T7726 T7723 prep towards,turned
R4522 T7727 T7726 pcomp addressing,towards
R4523 T7728 T7729 mark whether,involved
R4524 T7729 T7727 ccomp involved,addressing
R4525 T7730 T7731 compound TGF,β2
R4526 T7731 T7729 nsubjpass β2,involved
R4527 T7732 T7731 punct -,β2
R4528 T7733 T7729 aux might,involved
R4529 T7734 T7729 auxpass be,involved
R4530 T7735 T7729 prep in,involved
R4531 T7736 T7735 pcomp regulating,in
R4532 T7737 T7738 compound Snail,expression
R4533 T7738 T7736 dobj expression,regulating
R4534 T7739 T7736 prep in,regulating
R4535 T7740 T7739 pobj keratinocytes,in
R4536 T7741 T7740 acl isolated,keratinocytes
R4537 T7742 T7741 prep from,isolated
R4538 T7743 T7744 det the,layer
R4539 T7744 T7742 pobj layer,from
R4540 T7745 T7744 amod basal,layer
R4541 T7746 T7744 prep of,layer
R4542 T7747 T7748 det the,epidermis
R4543 T7748 T7746 pobj epidermis,of
R4544 T7749 T7723 punct .,turned
R4545 T7751 T7752 mark Though,is
R4546 T7752 T7754 advcl is,are
R4547 T7753 T7752 expl there,is
R4548 T7755 T7756 det no,system
R4549 T7756 T7752 attr system,is
R4550 T7757 T7758 compound cell,culture
R4551 T7758 T7756 compound culture,system
R4552 T7759 T7756 amod available,system
R4553 T7760 T7761 aux to,study
R4554 T7761 T7759 xcomp study,available
R4555 T7762 T7761 advmod specifically,study
R4556 T7763 T7764 amod placodal,cells
R4557 T7764 T7761 dobj cells,study
R4558 T7765 T7754 punct ", ",are
R4559 T7766 T7767 det these,keratinocytes
R4560 T7767 T7754 nsubj keratinocytes,are
R4561 T7768 T7769 poss their,progenitors
R4562 T7769 T7754 attr progenitors,are
R4563 T7770 T7754 cc and,are
R4564 T7771 T7754 conj are,are
R4565 T7772 T7773 det the,approximation
R4566 T7773 T7771 attr approximation,are
R4567 T7774 T7773 amod closest,approximation
R4568 T7775 T7773 amod available,approximation
R4569 T7776 T7777 aux to,study
R4570 T7777 T7775 xcomp study,available
R4571 T7778 T7779 det the,behavior
R4572 T7779 T7777 dobj behavior,study
R4573 T7780 T7779 prep of,behavior
R4574 T7781 T7782 amod epithelial,cells
R4575 T7782 T7780 pobj cells,of
R4576 T7783 T7782 prep of,cells
R4577 T7784 T7785 det the,placode
R4578 T7785 T7783 pobj placode,of
R4579 T7786 T7754 punct .,are
R4580 T7788 T7789 advmod Interestingly,caused
R4581 T7790 T7789 punct ", ",caused
R4582 T7791 T7789 nsubj treatment,caused
R4583 T7792 T7791 prep of,treatment
R4584 T7793 T7794 amod cultured,keratinocytes
R4585 T7794 T7792 pobj keratinocytes,of
R4586 T7795 T7791 prep with,treatment
R4587 T7796 T7797 advmod as,5
R4588 T7797 T7800 nummod 5,ng
R4589 T7798 T7797 amod little,5
R4590 T7799 T7797 quantmod as,5
R4591 T7800 T7795 pobj ng,with
R4592 T7801 T7802 punct /,ml
R4593 T7802 T7800 prep ml,ng
R4594 T7803 T7800 prep of,ng
R4595 T7804 T7805 compound TGF,β2
R4596 T7805 T7803 pobj β2,of
R4597 T7806 T7805 punct -,β2
R4598 T7807 T7808 det a,induction
R4599 T7808 T7789 dobj induction,caused
R4600 T7809 T7808 amod rapid,induction
R4601 T7810 T7809 cc and,rapid
R4602 T7811 T7809 conj transient,rapid
R4603 T7812 T7808 prep of,induction
R4604 T7813 T7812 pobj Snail,of
R4605 T7814 T7815 punct (,5B
R4606 T7815 T7789 parataxis 5B,caused
R4607 T7816 T7815 compound Figure,5B
R4608 T7817 T7815 punct ),5B
R4609 T7818 T7789 punct .,caused
R4610 T7820 T7821 prep Following,detected
R4611 T7822 T7823 det this,treatment
R4612 T7823 T7820 pobj treatment,Following
R4613 T7824 T7821 punct ", ",detected
R4614 T7825 T7826 compound Snail,protein
R4615 T7826 T7821 nsubjpass protein,detected
R4616 T7827 T7821 auxpass was,detected
R4617 T7828 T7821 prep within,detected
R4618 T7829 T7830 nummod 30,min
R4619 T7830 T7828 pobj min,within
R4620 T7831 T7821 punct ", ",detected
R4621 T7832 T7821 conj peaked,detected
R4622 T7833 T7832 prep at,peaked
R4623 T7834 T7835 nummod 2,h
R4624 T7835 T7833 pobj h,at
R4625 T7836 T7832 punct ", ",peaked
R4626 T7837 T7832 cc and,peaked
R4627 T7838 T7839 advmod then,declined
R4628 T7839 T7832 conj declined,peaked
R4629 T7840 T7839 advmod thereafter,declined
R4630 T7841 T7821 punct .,detected
R4631 T7843 T7844 det The,induction
R4632 T7844 T7845 nsubj induction,appeared
R4633 T7846 T7844 prep of,induction
R4634 T7847 T7846 pobj Snail,of
R4635 T7848 T7849 aux to,be
R4636 T7849 T7845 xcomp be,appeared
R4637 T7850 T7849 acomp specific,be
R4638 T7851 T7850 prep for,specific
R4639 T7852 T7853 det the,form
R4640 T7853 T7851 pobj form,for
R4641 T7854 T7853 amod active,form
R4642 T7855 T7853 prep of,form
R4643 T7856 T7857 det the,factor
R4644 T7857 T7855 pobj factor,of
R4645 T7858 T7857 compound growth,factor
R4646 T7859 T7849 punct ", ",be
R4647 T7860 T7861 mark as,obliterated
R4648 T7861 T7849 advcl obliterated,be
R4649 T7862 T7861 nsubj pretreatment,obliterated
R4650 T7863 T7862 prep of,pretreatment
R4651 T7864 T7865 compound TGF,β2
R4652 T7865 T7863 pobj β2,of
R4653 T7866 T7865 punct -,β2
R4654 T7867 T7862 prep for,pretreatment
R4655 T7868 T7869 nummod 10,min
R4656 T7869 T7867 pobj min,for
R4657 T7870 T7862 prep at,pretreatment
R4658 T7871 T7872 nummod 100,°C
R4659 T7872 T7870 pobj °C,at
R4660 T7873 T7874 det the,response
R4661 T7874 T7861 dobj response,obliterated
R4662 T7875 T7876 punct [,lanes
R4663 T7876 T7849 parataxis lanes,be
R4664 T7877 T7878 compound Figure,5B
R4665 T7878 T7876 dep 5B,lanes
R4666 T7879 T7876 punct ", ",lanes
R4667 T7880 T7876 acl marked,lanes
R4668 T7881 T7880 punct (,marked
R4669 T7882 T7880 oprd –,marked
R4670 T7883 T7876 punct ),lanes
R4671 T7884 T7876 punct ],lanes
R4672 T7885 T7845 punct .,appeared
R4673 T7887 T7888 prep By,maintained
R4674 T7889 T7887 pobj contrast,By
R4675 T7890 T7888 punct ", ",maintained
R4676 T7891 T7892 mark although,remained
R4677 T7892 T7888 advcl remained,maintained
R4678 T7893 T7894 compound TGF,β
R4679 T7894 T7896 compound β,receptor
R4680 T7895 T7894 punct -,β
R4681 T7896 T7897 compound receptor,activation
R4682 T7897 T7892 nsubj activation,remained
R4683 T7898 T7892 acomp elevated,remained
R4684 T7899 T7892 prep during,remained
R4685 T7900 T7901 det the,duration
R4686 T7901 T7899 pobj duration,during
R4687 T7902 T7901 prep of,duration
R4688 T7903 T7904 det the,experiment
R4689 T7904 T7902 pobj experiment,of
R4690 T7905 T7892 punct (,remained
R4691 T7906 T7907 mark as,measured
R4692 T7907 T7892 advcl measured,remained
R4693 T7908 T7907 prep by,measured
R4694 T7909 T7910 det the,phosphorylation
R4695 T7910 T7908 pobj phosphorylation,by
R4696 T7911 T7910 amod sustained,phosphorylation
R4697 T7912 T7910 prep of,phosphorylation
R4698 T7913 T7914 det the,effector
R4699 T7914 T7912 pobj effector,of
R4700 T7915 T7914 amod downstream,effector
R4701 T7916 T7914 appos SMAD2,effector
R4702 T7917 T7888 punct ),maintained
R4703 T7918 T7919 compound Snail,expression
R4704 T7919 T7888 nsubjpass expression,maintained
R4705 T7920 T7888 aux could,maintained
R4706 T7921 T7888 neg not,maintained
R4707 T7922 T7888 auxpass be,maintained
R4708 T7923 T7924 punct (,5B
R4709 T7924 T7888 parataxis 5B,maintained
R4710 T7925 T7924 compound Figure,5B
R4711 T7926 T7924 punct ),5B
R4712 T7927 T7888 punct .,maintained
R4713 T7929 T7930 advmod Thus,seemed
R4714 T7931 T7930 punct ", ",seemed
R4715 T7932 T7933 mark although,correlated
R4716 T7933 T7930 advcl correlated,seemed
R4717 T7934 T7935 compound Snail,expression
R4718 T7935 T7933 nsubj expression,correlated
R4719 T7936 T7933 prep with,correlated
R4720 T7937 T7938 amod phosphorylated,SMAD2
R4721 T7938 T7939 nmod SMAD2,induction
R4722 T7939 T7936 pobj induction,with
R4723 T7940 T7938 punct (,SMAD2
R4724 T7941 T7938 appos pSMAD2,SMAD2
R4725 T7942 T7939 punct ),induction
R4726 T7943 T7930 punct ", ",seemed
R4727 T7944 T7945 poss its,decline
R4728 T7945 T7930 nsubj decline,seemed
R4729 T7946 T7947 aux to,rely
R4730 T7947 T7930 xcomp rely,seemed
R4731 T7948 T7947 prep on,rely
R4732 T7949 T7950 amod secondary,events
R4733 T7950 T7948 pobj events,on
R4734 T7951 T7950 amod downstream,events
R4735 T7952 T7930 punct .,seemed
R4736 T7954 T7955 det The,kinetics
R4737 T7955 T7957 nsubjpass kinetics,reflected
R4738 T7956 T7955 amod rapid,kinetics
R4739 T7958 T7955 prep of,kinetics
R4740 T7959 T7960 compound Snail,expression
R4741 T7960 T7958 pobj expression,of
R4742 T7961 T7957 auxpass were,reflected
R4743 T7962 T7957 prep at,reflected
R4744 T7963 T7964 det the,level
R4745 T7964 T7962 pobj level,at
R4746 T7965 T7964 compound mRNA,level
R4747 T7966 T7957 punct ", ",reflected
R4748 T7967 T7957 advcl suggesting,reflected
R4749 T7968 T7969 mark that,be
R4750 T7969 T7967 ccomp be,suggesting
R4751 T7970 T7971 compound Snail,promoter
R4752 T7971 T7972 compound promoter,activity
R4753 T7972 T7969 nsubj activity,be
R4754 T7973 T7972 prep in,activity
R4755 T7974 T7973 pobj keratinocytes,in
R4756 T7975 T7969 aux might,be
R4757 T7976 T7969 acomp sensitive,be
R4758 T7977 T7976 prep to,sensitive
R4759 T7978 T7979 compound TGF,β2
R4760 T7979 T7981 compound β2,signaling
R4761 T7980 T7979 punct -,β2
R4762 T7981 T7977 pobj signaling,to
R4763 T7982 T7983 punct (,5C
R4764 T7983 T7957 parataxis 5C,reflected
R4765 T7984 T7983 compound Figure,5C
R4766 T7985 T7983 punct ),5C
R4767 T7986 T7957 punct .,reflected
R4768 T7988 T7989 aux To,test
R4769 T7989 T7990 advcl test,engineered
R4770 T7991 T7992 det this,possibility
R4771 T7992 T7989 dobj possibility,test
R4772 T7993 T7990 punct ", ",engineered
R4773 T7994 T7990 nsubj we,engineered
R4774 T7995 T7996 det a,transgene
R4775 T7996 T7990 dobj transgene,engineered
R4776 T7997 T7996 acl driving,transgene
R4777 T7998 T7999 det the,reporter
R4778 T7999 T7997 dobj reporter,driving
R4779 T8000 T8001 compound β,galactosidase
R4780 T8001 T7999 compound galactosidase,reporter
R4781 T8002 T8001 punct -,galactosidase
R4782 T8003 T7997 prep under,driving
R4783 T8004 T8005 det the,control
R4784 T8005 T8003 pobj control,under
R4785 T8006 T8005 prep of,control
R4786 T8007 T8008 advmod approximately,2.2
R4787 T8008 T8009 nummod 2.2,kb
R4788 T8009 T8006 pobj kb,of
R4789 T8010 T8009 prep of,kb
R4790 T8011 T8012 compound promoter,sequence
R4791 T8012 T8010 pobj sequence,of
R4792 T8013 T8012 acl located,sequence
R4793 T8014 T8013 npadvmod 5,located
R4794 T8015 T8014 punct ′,5
R4795 T8016 T8014 prep from,5
R4796 T8017 T8018 det the,site
R4797 T8018 T8016 pobj site,from
R4798 T8019 T8018 compound transcription,site
R4799 T8020 T8018 compound initiation,site
R4800 T8021 T8018 prep of,site
R4801 T8022 T8023 det the,gene
R4802 T8023 T8021 pobj gene,of
R4803 T8024 T8023 compound mouse,gene
R4804 T8025 T8023 compound Snail,gene
R4805 T8026 T7990 punct .,engineered
R4806 T8028 T8029 prep At,treated
R4807 T8030 T8031 nummod 2,d
R4808 T8031 T8028 pobj d,At
R4809 T8032 T8031 prep after,d
R4810 T8033 T8034 amod transient,transfection
R4811 T8034 T8032 pobj transfection,after
R4812 T8035 T8029 punct ", ",treated
R4813 T8036 T8029 nsubjpass keratinocytes,treated
R4814 T8037 T8029 auxpass were,treated
R4815 T8038 T8029 prep with,treated
R4816 T8039 T8040 compound TGF,β2
R4817 T8040 T8038 pobj β2,with
R4818 T8041 T8040 punct -,β2
R4819 T8042 T8043 punct (,0
R4820 T8043 T8029 parataxis 0,treated
R4821 T8044 T8043 nsubj t,0
R4822 T8045 T8043 punct =,0
R4823 T8046 T8043 punct ),0
R4824 T8047 T8029 cc and,treated
R4825 T8048 T8049 advmod then,assayed
R4826 T8049 T8029 conj assayed,treated
R4827 T8050 T8049 prep for,assayed
R4828 T8051 T8052 compound transgene,activity
R4829 T8052 T8050 pobj activity,for
R4830 T8053 T8049 prep over,assayed
R4831 T8054 T8055 det the,course
R4832 T8055 T8053 pobj course,over
R4833 T8056 T8055 amod same,course
R4834 T8057 T8055 compound time,course
R4835 T8058 T8059 prep in,observed
R4836 T8059 T8055 relcl observed,course
R4837 T8060 T8058 pobj which,in
R4838 T8061 T8059 nsubj we,observed
R4839 T8062 T8059 aux had,observed
R4840 T8063 T8064 compound Snail,protein
R4841 T8064 T8065 compound protein,induction
R4842 T8065 T8059 dobj induction,observed
R4843 T8066 T8029 punct .,treated
R4844 T8068 T8069 det The,results
R4845 T8069 T8070 nsubjpass results,presented
R4846 T8071 T8069 prep of,results
R4847 T8072 T8073 det this,experiment
R4848 T8073 T8071 pobj experiment,of
R4849 T8074 T8070 auxpass are,presented
R4850 T8075 T8070 prep in,presented
R4851 T8076 T8077 compound Figure,5D
R4852 T8077 T8075 pobj 5D,in
R4853 T8078 T8070 punct .,presented
R4854 T8080 T8081 prep Within,increased
R4855 T8082 T8083 nummod 0.5,h
R4856 T8083 T8080 pobj h,Within
R4857 T8084 T8083 prep of,h
R4858 T8085 T8086 compound TGF,β2
R4859 T8086 T8088 compound β2,treatment
R4860 T8087 T8086 punct -,β2
R4861 T8088 T8084 pobj treatment,of
R4862 T8089 T8081 punct ", ",increased
R4863 T8090 T8091 compound Snail,promoter
R4864 T8091 T8092 compound promoter,activity
R4865 T8092 T8081 nsubj activity,increased
R4866 T8093 T8081 aux had,increased
R4867 T8094 T8081 advmod 3-fold,increased
R4868 T8095 T8081 punct ", ",increased
R4869 T8096 T8081 cc and,increased
R4870 T8097 T8098 prep by,peaked
R4871 T8098 T8081 conj peaked,increased
R4872 T8099 T8100 nummod 2,h
R4873 T8100 T8097 pobj h,by
R4874 T8101 T8098 punct ", ",peaked
R4875 T8102 T8098 nsubj it,peaked
R4876 T8103 T8098 prep to,peaked
R4877 T8104 T8105 advmod approximately,10-fold
R4878 T8105 T8103 pcomp 10-fold,to
R4879 T8106 T8105 prep over,10-fold
R4880 T8107 T8108 compound control,levels
R4881 T8108 T8106 pobj levels,over
R4882 T8109 T8110 punct (,5D
R4883 T8110 T8098 parataxis 5D,peaked
R4884 T8111 T8110 compound Figure,5D
R4885 T8112 T8110 punct ),5D
R4886 T8113 T8098 punct .,peaked
R4887 T8115 T8116 advmod Thereafter,returned
R4888 T8117 T8116 punct ", ",returned
R4889 T8118 T8119 compound Snail,promoter
R4890 T8119 T8120 compound promoter,activity
R4891 T8120 T8116 nsubj activity,returned
R4892 T8121 T8116 advmod rapidly,returned
R4893 T8122 T8116 prep to,returned
R4894 T8123 T8124 det the,levels
R4895 T8124 T8122 pobj levels,to
R4896 T8125 T8124 amod basal,levels
R4897 T8126 T8124 acl seen,levels
R4898 T8127 T8126 prep in,seen
R4899 T8128 T8129 amod unstimulated,keratinocytes
R4900 T8129 T8127 pobj keratinocytes,in
R4901 T8130 T8116 punct .,returned
R4902 T8132 T8133 det The,kinetics
R4903 T8133 T8134 nsubj kinetics,paralleled
R4904 T8135 T8133 prep of,kinetics
R4905 T8136 T8137 compound Snail,promoter
R4906 T8137 T8138 compound promoter,activity
R4907 T8138 T8135 pobj activity,of
R4908 T8139 T8134 advmod closely,paralleled
R4909 T8140 T8134 dobj those,paralleled
R4910 T8141 T8140 acl observed,those
R4911 T8142 T8141 prep for,observed
R4912 T8143 T8144 compound Snail,protein
R4913 T8144 T8145 compound protein,induction
R4914 T8145 T8142 pobj induction,for
R4915 T8146 T8134 punct .,paralleled
R4916 T8148 T8149 advmod Moreover,appeared
R4917 T8150 T8149 punct ", ",appeared
R4918 T8151 T8152 det the,effects
R4919 T8152 T8149 nsubj effects,appeared
R4920 T8153 T8152 amod stimulatory,effects
R4921 T8154 T8155 aux to,be
R4922 T8155 T8149 xcomp be,appeared
R4923 T8156 T8155 acomp specific,be
R4924 T8157 T8156 prep to,specific
R4925 T8158 T8159 compound TGF,β2
R4926 T8159 T8157 pobj β2,to
R4927 T8160 T8159 punct -,β2
R4928 T8161 T8155 punct ", ",be
R4929 T8162 T8163 mark since,abrogated
R4930 T8163 T8155 advcl abrogated,be
R4931 T8164 T8163 nsubjpass they,abrogated
R4932 T8165 T8163 auxpass were,abrogated
R4933 T8166 T8167 preconj either,by
R4934 T8167 T8163 prep by,abrogated
R4935 T8168 T8169 compound heat,inactivation
R4936 T8169 T8167 pobj inactivation,by
R4937 T8170 T8169 prep of,inactivation
R4938 T8171 T8172 det the,protein
R4939 T8172 T8170 pobj protein,of
R4940 T8173 T8174 compound TGF,β2
R4941 T8174 T8172 compound β2,protein
R4942 T8175 T8174 punct -,β2
R4943 T8176 T8167 cc or,by
R4944 T8177 T8167 conj by,by
R4945 T8178 T8177 pobj mutation,by
R4946 T8179 T8178 prep of,mutation
R4947 T8180 T8181 det a,element
R4948 T8181 T8179 pobj element,of
R4949 T8182 T8181 amod putative,element
R4950 T8183 T8184 compound SMAD,binding
R4951 T8184 T8181 compound binding,element
R4952 T8185 T8181 acl located,element
R4953 T8186 T8187 quantmod about,1.8
R4954 T8187 T8188 nummod 1.8,kb
R4955 T8188 T8185 npadvmod kb,located
R4956 T8189 T8188 nummod 5,kb
R4957 T8190 T8188 punct ′,kb
R4958 T8191 T8188 prep from,kb
R4959 T8192 T8193 det the,site
R4960 T8193 T8191 pobj site,from
R4961 T8194 T8193 compound Snail,site
R4962 T8195 T8193 compound transcription,site
R4963 T8196 T8193 compound start,site
R4964 T8197 T8198 punct (,5D
R4965 T8198 T8163 parataxis 5D,abrogated
R4966 T8199 T8198 compound Figure,5D
R4967 T8200 T8198 punct ),5D
R4968 T8201 T8149 punct .,appeared
R4969 T8203 T8204 advcl Taken,suggested
R4970 T8205 T8203 advmod together,Taken
R4971 T8206 T8204 punct ", ",suggested
R4972 T8207 T8208 det these,results
R4973 T8208 T8204 nsubj results,suggested
R4974 T8209 T8210 mark that,results
R4975 T8210 T8204 advcl results,suggested
R4976 T8211 T8210 prep in,results
R4977 T8212 T8211 pobj keratinocytes,in
R4978 T8213 T8210 punct ", ",results
R4979 T8214 T8215 compound TGF,β2
R4980 T8215 T8217 compound β2,signaling
R4981 T8216 T8215 punct -,β2
R4982 T8217 T8210 nsubj signaling,results
R4983 T8218 T8210 prep in,results
R4984 T8219 T8220 det a,activation
R4985 T8220 T8218 pobj activation,in
R4986 T8221 T8222 npadvmod pSMAD2,dependent
R4987 T8222 T8220 amod dependent,activation
R4988 T8223 T8222 punct -,dependent
R4989 T8224 T8220 amod transient,activation
R4990 T8225 T8220 prep of,activation
R4991 T8226 T8227 det the,gene
R4992 T8227 T8225 pobj gene,of
R4993 T8228 T8227 compound Snail,gene
R4994 T8229 T8210 punct ", ",results
R4995 T8230 T8210 cc and,results
R4996 T8231 T8232 mark that,relies
R4997 T8232 T8210 conj relies,results
R4998 T8233 T8232 nsubj maintenance,relies
R4999 T8234 T8233 prep of,maintenance
R5000 T8235 T8236 compound Snail,protein
R5001 T8236 T8234 pobj protein,of
R5002 T8237 T8232 punct ", ",relies
R5003 T8238 T8232 prep in,relies
R5004 T8239 T8238 pobj part,in
R5005 T8240 T8232 punct ", ",relies
R5006 T8241 T8232 prep upon,relies
R5007 T8242 T8243 amod sustained,activity
R5008 T8243 T8241 pobj activity,upon
R5009 T8244 T8243 compound promoter,activity
R5010 T8245 T8204 punct .,suggested
R5011 T8247 T8248 det The,brevity
R5012 T8248 T8249 nsubj brevity,resembled
R5013 T8250 T8248 prep of,brevity
R5014 T8251 T8252 nmod Snail,gene
R5015 T8252 T8253 nmod gene,induction
R5016 T8253 T8250 pobj induction,of
R5017 T8254 T8252 cc and,gene
R5018 T8255 T8252 conj protein,gene
R5019 T8256 T8253 prep in,induction
R5020 T8257 T8258 compound TGF,β2
R5021 T8258 T8260 npadvmod β2,treated
R5022 T8259 T8258 punct -,β2
R5023 T8260 T8261 amod treated,keratinocytes
R5024 T8261 T8256 pobj keratinocytes,in
R5025 T8262 T8261 amod cultured,keratinocytes
R5026 T8263 T8264 det the,appearance
R5027 T8264 T8249 dobj appearance,resembled
R5028 T8265 T8264 amod temporal,appearance
R5029 T8266 T8264 prep of,appearance
R5030 T8267 T8268 compound Snail,mRNA
R5031 T8268 T8266 pobj mRNA,of
R5032 T8269 T8268 cc and,mRNA
R5033 T8270 T8268 conj protein,mRNA
R5034 T8271 T8264 prep at,appearance
R5035 T8272 T8273 det the,initiation
R5036 T8273 T8271 pobj initiation,at
R5037 T8274 T8273 prep of,initiation
R5038 T8275 T8276 compound hair,follicle
R5039 T8276 T8277 compound follicle,morphogenesis
R5040 T8277 T8274 pobj morphogenesis,of
R5041 T8278 T8249 prep in,resembled
R5042 T8279 T8280 amod embryonic,skin
R5043 T8280 T8278 pobj skin,in
R5044 T8281 T8280 compound mouse,skin
R5045 T8282 T8249 punct .,resembled
R5046 T8284 T8285 aux To,test
R5047 T8285 T8286 advcl test,analyzed
R5048 T8287 T8288 mark whether,required
R5049 T8288 T8285 ccomp required,test
R5050 T8289 T8290 compound TGF,β2
R5051 T8290 T8288 nsubjpass β2,required
R5052 T8291 T8290 punct -,β2
R5053 T8292 T8288 aux might,required
R5054 T8293 T8288 auxpass be,required
R5055 T8294 T8288 prep for,required
R5056 T8295 T8296 compound Snail,induction
R5057 T8296 T8294 pobj induction,for
R5058 T8297 T8296 prep in,induction
R5059 T8298 T8299 compound hair,bud
R5060 T8299 T8300 compound bud,formation
R5061 T8300 T8297 pobj formation,in
R5062 T8301 T8302 advmod in,vivo
R5063 T8302 T8288 advmod vivo,required
R5064 T8303 T8286 punct ", ",analyzed
R5065 T8304 T8286 nsubj we,analyzed
R5066 T8305 T8286 advmod first,analyzed
R5067 T8306 T8307 mark whether,expressed
R5068 T8307 T8286 ccomp expressed,analyzed
R5069 T8308 T8309 compound TGF,β2
R5070 T8309 T8307 nsubjpass β2,expressed
R5071 T8310 T8309 punct -,β2
R5072 T8311 T8307 auxpass was,expressed
R5073 T8312 T8307 prep in,expressed
R5074 T8313 T8312 cc or,in
R5075 T8314 T8312 conj around,in
R5076 T8315 T8316 det the,bud
R5077 T8316 T8314 pobj bud,around
R5078 T8317 T8316 compound hair,bud
R5079 T8318 T8286 punct .,analyzed
R5080 T8320 T8321 advcl Consistent,labeled
R5081 T8322 T8320 prep with,Consistent
R5082 T8323 T8324 amod previous,observations
R5083 T8324 T8322 pobj observations,with
R5084 T8325 T8326 punct [,33
R5085 T8326 T8320 parataxis 33,Consistent
R5086 T8327 T8326 punct ],33
R5087 T8328 T8321 punct ", ",labeled
R5088 T8329 T8330 det an,antibody
R5089 T8330 T8321 nsubj antibody,labeled
R5090 T8331 T8332 amod anti-TGF,β2
R5091 T8332 T8330 compound β2,antibody
R5092 T8333 T8332 punct -,β2
R5093 T8334 T8335 amod developing,buds
R5094 T8335 T8321 dobj buds,labeled
R5095 T8336 T8335 compound hair,buds
R5096 T8337 T8338 punct (,6A
R5097 T8338 T8321 parataxis 6A,labeled
R5098 T8339 T8338 compound Figure,6A
R5099 T8340 T8338 punct ),6A
R5100 T8341 T8321 punct .,labeled
R5101 T8343 T8344 det This,labeling
R5102 T8344 T8345 nsubj labeling,appeared
R5103 T8346 T8347 aux to,be
R5104 T8347 T8345 xcomp be,appeared
R5105 T8348 T8347 acomp specific,be
R5106 T8349 T8350 mark as,judged
R5107 T8350 T8347 advcl judged,be
R5108 T8351 T8350 prep by,judged
R5109 T8352 T8353 det the,lack
R5110 T8353 T8351 pobj lack,by
R5111 T8354 T8353 prep of,lack
R5112 T8355 T8354 pobj staining,of
R5113 T8356 T8353 prep in,lack
R5114 T8357 T8358 compound follicle,buds
R5115 T8358 T8356 pobj buds,in
R5116 T8359 T8358 prep from,buds
R5117 T8360 T8359 pobj mice,from
R5118 T8361 T8360 amod homozygous,mice
R5119 T8362 T8361 prep for,homozygous
R5120 T8363 T8364 det a,mutation
R5121 T8364 T8362 pobj mutation,for
R5122 T8365 T8366 nmod TGF,β2
R5123 T8366 T8364 nmod β2,mutation
R5124 T8367 T8366 punct -,β2
R5125 T8368 T8364 amod null,mutation
R5126 T8369 T8370 punct (,34
R5127 T8370 T8345 parataxis 34,appeared
R5128 T8371 T8372 compound Figure,6A
R5129 T8372 T8370 dep 6A,34
R5130 T8373 T8370 punct ;,34
R5131 T8374 T8370 punct [,34
R5132 T8375 T8370 punct ],34
R5133 T8376 T8370 punct ),34
R5134 T8377 T8345 punct .,appeared
R5135 T8379 T8380 advmod Moreover,expressed
R5136 T8381 T8380 punct ", ",expressed
R5137 T8382 T8383 det the,effector
R5138 T8383 T8380 nsubjpass effector,expressed
R5139 T8384 T8383 amod downstream,effector
R5140 T8385 T8383 prep of,effector
R5141 T8386 T8387 compound TGF,β2
R5142 T8387 T8389 compound β2,signaling
R5143 T8388 T8387 punct -,β2
R5144 T8389 T8385 pobj signaling,of
R5145 T8390 T8383 punct ", ",effector
R5146 T8391 T8383 appos pSMAD2,effector
R5147 T8392 T8380 punct ", ",expressed
R5148 T8393 T8380 auxpass was,expressed
R5149 T8394 T8380 advmod also,expressed
R5150 T8395 T8380 prep in,expressed
R5151 T8396 T8397 nmod WT,buds
R5152 T8397 T8395 pobj buds,in
R5153 T8398 T8396 punct ", ",WT
R5154 T8399 T8396 cc but,WT
R5155 T8400 T8399 neg not,but
R5156 T8401 T8402 compound TGF,β2
R5157 T8402 T8404 npadvmod β2,null
R5158 T8403 T8402 punct -,β2
R5159 T8404 T8396 conj null,WT
R5160 T8405 T8404 punct -,null
R5161 T8406 T8397 punct ", ",buds
R5162 T8407 T8397 compound hair,buds
R5163 T8408 T8409 punct (,6B
R5164 T8409 T8380 parataxis 6B,expressed
R5165 T8410 T8409 compound Figure,6B
R5166 T8411 T8409 punct ),6B
R5167 T8412 T8380 punct .,expressed
R5168 T8414 T8415 advmod Together,underscore
R5169 T8416 T8415 punct ", ",underscore
R5170 T8417 T8418 det these,data
R5171 T8418 T8415 nsubj data,underscore
R5172 T8419 T8420 det the,importance
R5173 T8420 T8415 dobj importance,underscore
R5174 T8421 T8420 prep of,importance
R5175 T8422 T8423 det the,isoform
R5176 T8423 T8421 pobj isoform,of
R5177 T8424 T8425 compound TGF,β2
R5178 T8425 T8423 compound β2,isoform
R5179 T8426 T8425 punct -,β2
R5180 T8427 T8415 prep despite,underscore
R5181 T8428 T8427 pobj expression,despite
R5182 T8429 T8428 prep of,expression
R5183 T8430 T8431 preconj both,β1
R5184 T8431 T8429 pobj β1,of
R5185 T8432 T8431 compound TGF,β1
R5186 T8433 T8431 punct -,β1
R5187 T8434 T8431 cc and,β1
R5188 T8435 T8436 compound TGF,β2
R5189 T8436 T8431 conj β2,β1
R5190 T8437 T8436 punct -,β2
R5191 T8438 T8428 prep in,expression
R5192 T8439 T8440 amod developing,buds
R5193 T8440 T8438 pobj buds,in
R5194 T8441 T8440 compound hair,buds
R5195 T8442 T8428 prep at,expression
R5196 T8443 T8444 det this,stage
R5197 T8444 T8442 pobj stage,at
R5198 T8445 T8415 punct .,underscore
R5199 T8447 T8448 aux To,explore
R5200 T8448 T8450 advcl explore,examined
R5201 T8449 T8448 advmod further,explore
R5202 T8451 T8452 det the,relation
R5203 T8452 T8448 dobj relation,explore
R5204 T8453 T8452 amod possible,relation
R5205 T8454 T8452 prep between,relation
R5206 T8455 T8454 pobj Snail,between
R5207 T8456 T8455 cc and,Snail
R5208 T8457 T8458 compound TGF,β2
R5209 T8458 T8455 conj β2,Snail
R5210 T8459 T8458 punct -,β2
R5211 T8460 T8450 punct ", ",examined
R5212 T8461 T8450 nsubj we,examined
R5213 T8462 T8463 det the,status
R5214 T8463 T8450 dobj status,examined
R5215 T8464 T8463 prep of,status
R5216 T8465 T8466 compound Snail,expression
R5217 T8466 T8464 pobj expression,of
R5218 T8467 T8463 prep in,status
R5219 T8468 T8469 compound TGF,β2
R5220 T8469 T8471 npadvmod β2,null
R5221 T8470 T8469 punct -,β2
R5222 T8471 T8473 amod null,buds
R5223 T8472 T8471 punct -,null
R5224 T8473 T8467 pobj buds,in
R5225 T8474 T8473 compound hair,buds
R5226 T8475 T8450 punct .,examined
R5227 T8477 T8478 mark As,judged
R5228 T8478 T8479 advcl judged,was
R5229 T8480 T8478 prep by,judged
R5230 T8481 T8480 pobj immunohistochemistry,by
R5231 T8482 T8479 punct ", ",was
R5232 T8483 T8484 compound Snail,protein
R5233 T8484 T8479 nsubj protein,was
R5234 T8485 T8479 acomp absent,was
R5235 T8486 T8485 prep from,absent
R5236 T8487 T8488 compound E17.5,skin
R5237 T8488 T8486 pobj skin,from
R5238 T8489 T8488 prep of,skin
R5239 T8490 T8491 compound TGF,β2
R5240 T8491 T8493 npadvmod β2,null
R5241 T8492 T8491 punct -,β2
R5242 T8493 T8495 amod null,embryos
R5243 T8494 T8493 punct -,null
R5244 T8495 T8489 pobj embryos,of
R5245 T8496 T8486 cc but,from
R5246 T8497 T8496 neg not,but
R5247 T8498 T8486 conj from,from
R5248 T8499 T8498 pobj that,from
R5249 T8500 T8499 prep of,that
R5250 T8501 T8502 compound control,littermates
R5251 T8502 T8500 pobj littermates,of
R5252 T8503 T8504 punct (,6C
R5253 T8504 T8479 parataxis 6C,was
R5254 T8505 T8504 compound Figure,6C
R5255 T8506 T8504 punct ),6C
R5256 T8507 T8479 punct .,was
R5257 T8509 T8510 det This,effect
R5258 T8510 T8511 nsubj effect,appeared
R5259 T8512 T8513 aux to,exerted
R5260 T8513 T8511 xcomp exerted,appeared
R5261 T8514 T8513 auxpass be,exerted
R5262 T8515 T8513 prep at,exerted
R5263 T8516 T8517 det the,level
R5264 T8517 T8515 pobj level,at
R5265 T8518 T8517 amod transcriptional,level
R5266 T8519 T8511 punct ", ",appeared
R5267 T8520 T8521 mark since,found
R5268 T8521 T8511 advcl found,appeared
R5269 T8522 T8523 compound Snail,mRNAs
R5270 T8523 T8521 nsubjpass mRNAs,found
R5271 T8524 T8521 auxpass were,found
R5272 T8525 T8521 advmod also,found
R5273 T8526 T8521 neg not,found
R5274 T8527 T8521 prep in,found
R5275 T8528 T8529 compound TGF,β2
R5276 T8529 T8531 npadvmod β2,null
R5277 T8530 T8529 punct -,β2
R5278 T8531 T8532 amod null,buds
R5279 T8532 T8527 pobj buds,in
R5280 T8533 T8532 compound hair,buds
R5281 T8534 T8521 prep under,found
R5282 T8535 T8534 pobj conditions,under
R5283 T8536 T8537 prep in,detected
R5284 T8537 T8535 relcl detected,conditions
R5285 T8538 T8536 pobj which,in
R5286 T8539 T8540 det the,signal
R5287 T8559 T8557 prep in,detected
R5288 T8540 T8537 nsubjpass signal,detected
R5289 T8541 T8537 auxpass was,detected
R5290 T8560 T8561 nummod 2,wk
R5291 T8561 T8563 npadvmod wk,old
R5292 T8542 T8537 advmod readily,detected
R5293 T8562 T8561 punct -,wk
R5294 T8563 T8565 amod old,mice
R5295 T8564 T8563 punct -,old
R5296 T8543 T8537 prep in,detected
R5297 T8565 T8559 pobj mice,in
R5298 T8566 T8567 compound K14,Smad2
R5299 T8567 T8565 compound Smad2,mice
R5300 T8568 T8567 punct -,Smad2
R5301 T8569 T8565 compound Tg,mice
R5302 T8544 T8545 det the,buds
R5303 T8570 T8565 punct ", ",mice
R5304 T8571 T8572 dep which,display
R5305 T8572 T8565 relcl display,mice
R5306 T8545 T8543 pobj buds,in
R5307 T8573 T8574 amod elevated,signaling
R5308 T8546 T8545 compound hair,buds
R5309 T8574 T8572 dobj signaling,display
R5310 T8575 T8576 compound TGF,β
R5311 T8547 T8545 prep of,buds
R5312 T8576 T8574 compound β,signaling
R5313 T8577 T8576 punct -,β
R5314 T8578 T8572 prep in,display
R5315 T8548 T8549 compound littermate,skin
R5316 T8549 T8547 pobj skin,of
R5317 T8579 T8578 pobj skin,in
R5318 T8580 T8581 punct [,35
R5319 T8550 T8551 punct (,6D
R5320 T8581 T8559 parataxis 35,in
R5321 T8582 T8581 punct ],35
R5322 T8583 T8557 punct ", ",detected
R5323 T8551 T8521 parataxis 6D,found
R5324 T8584 T8585 compound Snail,protein
R5325 T8585 T8557 nsubjpass protein,detected
R5326 T8552 T8551 compound Figure,6D
R5327 T8586 T8557 auxpass was,detected
R5328 T8587 T8557 advmod readily,detected
R5329 T8553 T8551 punct ),6D
R5330 T8588 T8557 prep by,detected
R5331 T8554 T8511 punct .,appeared
R5332 T8589 T8590 compound Western,blot
R5333 T8590 T8591 compound blot,analyses
R5334 T8591 T8588 pobj analyses,by
R5335 T8592 T8557 punct ", ",detected
R5336 T8593 T8594 advmod where,found
R5337 T8556 T8557 advmod Conversely,detected
R5338 T8594 T8557 advcl found,detected
R5339 T8595 T8594 nsubjpass it,found
R5340 T8596 T8594 auxpass was,found
R5341 T8597 T8594 neg not,found
R5342 T8558 T8557 punct ", ",detected
R5343 T8598 T8594 prep in,found
R5344 T8599 T8600 amod postnatal,skin
R5345 T8600 T8598 pobj skin,in
R5346 T8601 T8602 punct (,6E
R5347 T8664 T8660 advmod still,formed
R5348 T8602 T8557 parataxis 6E,detected
R5349 T8603 T8602 compound Figure,6E
R5350 T8604 T8602 punct ),6E
R5351 T8605 T8557 punct .,detected
R5352 T8607 T8608 advcl Taken,provide
R5353 T8609 T8607 advmod together,Taken
R5354 T8610 T8608 punct ", ",provide
R5355 T8611 T8612 det these,results
R5356 T8666 T8660 prep in,formed
R5357 T8612 T8608 nsubj results,provide
R5358 T8613 T8614 amod compelling,evidence
R5359 T8614 T8608 dobj evidence,provide
R5360 T8615 T8616 mark that,is
R5361 T8616 T8614 acl is,evidence
R5362 T8667 T8668 compound TGF,β2
R5363 T8617 T8618 compound TGF,β2
R5364 T8618 T8616 nsubj β2,is
R5365 T8619 T8618 punct -,β2
R5366 T8668 T8670 npadvmod β2,null
R5367 T8620 T8621 advmod functionally,important
R5368 T8621 T8616 acomp important,is
R5369 T8669 T8668 punct -,β2
R5370 T8622 T8621 prep for,important
R5371 T8623 T8622 pcomp inducing,for
R5372 T8624 T8625 compound Snail,expression
R5373 T8670 T8671 amod null,skin
R5374 T8625 T8623 dobj expression,inducing
R5375 T8626 T8625 compound gene,expression
R5376 T8627 T8623 prep in,inducing
R5377 T8671 T8666 pobj skin,in
R5378 T8628 T8629 det a,manner
R5379 T8629 T8627 pobj manner,in
R5380 T8672 T8665 punct ", ",reduced
R5381 T8630 T8631 npadvmod pSMAD,dependent
R5382 T8631 T8629 amod dependent,manner
R5383 T8632 T8631 punct -,dependent
R5384 T8673 T8674 poss their,number
R5385 T8633 T8623 prep in,inducing
R5386 T8634 T8635 amod developing,buds
R5387 T8635 T8633 pobj buds,in
R5388 T8674 T8665 nsubjpass number,reduced
R5389 T8636 T8635 compound hair,buds
R5390 T8637 T8608 punct .,provide
R5391 T8675 T8665 auxpass was,reduced
R5392 T8639 T8640 mark Whether,contributes
R5393 T8640 T8644 csubjpass contributes,addressed
R5394 T8676 T8665 prep by,reduced
R5395 T8641 T8642 compound pMARK,activity
R5396 T8642 T8640 nsubj activity,contributes
R5397 T8643 T8640 advmod also,contributes
R5398 T8677 T8678 advmod approximately,50
R5399 T8645 T8640 prep to,contributes
R5400 T8646 T8647 compound Snail,induction
R5401 T8647 T8645 pobj induction,to
R5402 T8648 T8644 auxpass was,addressed
R5403 T8649 T8644 neg not,addressed
R5404 T8678 T8679 nummod 50,%
R5405 T8650 T8644 prep in,addressed
R5406 T8651 T8652 det the,study
R5407 T8652 T8650 pobj study,in
R5408 T8679 T8676 pobj %,by
R5409 T8653 T8652 amod present,study
R5410 T8654 T8655 punct [,15
R5411 T8655 T8644 parataxis 15,addressed
R5412 T8680 T8681 punct [,32
R5413 T8656 T8655 punct ],15
R5414 T8657 T8644 punct .,addressed
R5415 T8681 T8665 parataxis 32,reduced
R5416 T8659 T8660 mark Although,formed
R5417 T8660 T8665 advcl formed,reduced
R5418 T8661 T8662 det some,buds
R5419 T8682 T8681 punct ],32
R5420 T8662 T8660 nsubj buds,formed
R5421 T8663 T8662 compound hair,buds
R5422 T8683 T8665 punct .,reduced
R5423 T8685 T8686 advmod Thus,appear
R5424 T8687 T8686 punct ", ",appear
R5425 T8770 T8772 compound β2,signaling
R5426 T8688 T8689 mark although,impacts
R5427 T8689 T8686 advcl impacts,appear
R5428 T8771 T8770 punct -,β2
R5429 T8772 T8766 nsubj signaling,appeared
R5430 T8690 T8691 det the,pathway
R5431 T8773 T8774 aux to,be
R5432 T8774 T8766 xcomp be,appeared
R5433 T8775 T8776 det an,factor
R5434 T8691 T8689 nsubj pathway,impacts
R5435 T8776 T8774 attr factor,be
R5436 T8777 T8776 amod important,factor
R5437 T8692 T8691 acl mediated,pathway
R5438 T8778 T8776 prep for,factor
R5439 T8779 T8780 det the,proliferation
R5440 T8693 T8692 prep by,mediated
R5441 T8780 T8778 pobj proliferation,for
R5442 T8781 T8780 amod early,proliferation
R5443 T8782 T8783 dep that,occurs
R5444 T8694 T8695 compound TGF,β2
R5445 T8783 T8780 relcl occurs,proliferation
R5446 T8784 T8783 prep in,occurs
R5447 T8695 T8697 compound β2,signaling
R5448 T8785 T8786 det the,buds
R5449 T8786 T8784 pobj buds,in
R5450 T8787 T8786 amod developing,buds
R5451 T8696 T8695 punct -,β2
R5452 T8788 T8786 compound hair,buds
R5453 T8789 T8774 punct ", ",be
R5454 T8790 T8791 mark as,judged
R5455 T8791 T8774 advcl judged,be
R5456 T8792 T8791 prep by,judged
R5457 T8793 T8794 amod anti-Ki67,immunofluorescence
R5458 T8697 T8693 pobj signaling,by
R5459 T8794 T8792 pobj immunofluorescence,by
R5460 T8795 T8793 cc and,anti-Ki67
R5461 T8698 T8699 det the,step
R5462 T8796 T8793 conj anti-pMAPK,anti-Ki67
R5463 T8797 T8798 punct (,6F
R5464 T8798 T8774 parataxis 6F,be
R5465 T8699 T8689 dobj step,impacts
R5466 T8799 T8798 compound Figure,6F
R5467 T8800 T8798 cc and,6F
R5468 T8801 T8798 conj 6G,6F
R5469 T8700 T8699 amod earliest,step
R5470 T8802 T8798 punct ),6F
R5471 T8803 T8766 punct .,appeared
R5472 T8701 T8699 prep of,step
R5473 T8805 T8806 mark If,stimulates
R5474 T8806 T8810 advcl stimulates,expected
R5475 T8702 T8703 amod epithelial,invagination
R5476 T8807 T8808 compound TGF,β2
R5477 T8808 T8806 nsubj β2,stimulates
R5478 T8809 T8808 punct -,β2
R5479 T8703 T8701 pobj invagination,of
R5480 T8811 T8812 compound Snail,expression
R5481 T8812 T8806 dobj expression,stimulates
R5482 T8704 T8686 punct ", ",appear
R5483 T8813 T8806 prep in,stimulates
R5484 T8814 T8815 amod developing,buds
R5485 T8815 T8813 pobj buds,in
R5486 T8705 T8686 nsubj it,appear
R5487 T8816 T8810 punct ", ",expected
R5488 T8817 T8810 nsubjpass loss,expected
R5489 T8818 T8817 prep of,loss
R5490 T8706 T8686 aux does,appear
R5491 T8819 T8820 det this,morphogen
R5492 T8820 T8818 pobj morphogen,of
R5493 T8707 T8686 neg not,appear
R5494 T8821 T8810 aux would,expected
R5495 T8822 T8810 auxpass be,expected
R5496 T8823 T8824 aux to,affect
R5497 T8708 T8709 aux to,be
R5498 T8824 T8810 xcomp affect,expected
R5499 T8825 T8826 det the,expression
R5500 T8826 T8824 dobj expression,affect
R5501 T8709 T8686 xcomp be,appear
R5502 T8827 T8826 prep of,expression
R5503 T8828 T8827 pobj genes,of
R5504 T8829 T8830 dep that,repressed
R5505 T8830 T8828 relcl repressed,genes
R5506 T8831 T8830 auxpass are,repressed
R5507 T8832 T8830 advmod typically,repressed
R5508 T8710 T8709 acomp essential,be
R5509 T8833 T8830 agent by,repressed
R5510 T8834 T8833 pobj Snail,by
R5511 T8835 T8810 punct .,expected
R5512 T8711 T8710 prep for,essential
R5513 T8712 T8713 compound bud,morphogenesis
R5514 T8837 T8838 mark Since,is
R5515 T8713 T8711 pobj morphogenesis,for
R5516 T8838 T8847 advcl is,investigated
R5517 T8839 T8840 det a,target
R5518 T8714 T8686 punct .,appear
R5519 T8840 T8838 nsubj target,is
R5520 T8841 T8840 amod major,target
R5521 T8842 T8840 prep for,target
R5522 T8843 T8844 npadvmod Snail,mediated
R5523 T8716 T8717 advcl Consistent,took
R5524 T8844 T8846 amod mediated,repression
R5526 T8846 T8842 pobj repression,for
R5527 T8718 T8716 prep with,Consistent
R5528 T8848 T8849 det the,gene
R5529 T8849 T8838 attr gene,is
R5531 T8719 T8720 det this,notion
R5532 T8720 T8718 pobj notion,with
R5533 T8721 T8717 punct ", ",took
R5534 T8851 T8849 compound cadherin,gene
R5535 T8722 T8723 compound basement,membrane
R5536 T8723 T8724 compound membrane,remodeling
R5537 T8852 T8851 punct -,cadherin
R5538 T8853 T8854 punct [,13
R5539 T8854 T8838 parataxis 13,is
R5540 T8724 T8717 nsubj remodeling,took
R5541 T8855 T8854 nummod 12,13
R5542 T8856 T8854 punct ",",13
R5543 T8725 T8717 advmod still,took
R5544 T8857 T8854 punct ],13
R5545 T8858 T8847 punct ", ",investigated
R5546 T8859 T8847 nsubj we,investigated
R5547 T8726 T8717 dobj place,took
R5548 T8860 T8861 det the,status
R5549 T8861 T8847 dobj status,investigated
R5550 T8727 T8717 prep in,took
R5551 T8862 T8861 prep of,status
R5552 T8863 T8864 compound E,cadherin
R5553 T8864 T8862 pobj cadherin,of
R5554 T8728 T8729 det the,buds
R5555 T8865 T8864 punct -,cadherin
R5556 T8866 T8847 prep in,investigated
R5557 T8867 T8868 compound TGF,β2
R5558 T8868 T8870 npadvmod β2,null
R5559 T8869 T8868 punct -,β2
R5560 T8870 T8872 amod null,buds
R5561 T8729 T8727 pobj buds,in
R5562 T8871 T8870 punct -,null
R5563 T8872 T8866 pobj buds,in
R5564 T8873 T8847 punct .,investigated
R5565 T8730 T8731 compound TGF,β2
R5566 T8875 T8876 mark As,shown
R5567 T8731 T8733 npadvmod β2,null
R5568 T8732 T8731 punct -,β2
R5569 T8733 T8729 amod null,buds
R5570 T8734 T8733 punct -,null
R5571 T8876 T8877 advcl shown,displayed
R5572 T8735 T8717 punct ", ",took
R5573 T8878 T8876 prep in,shown
R5574 T8736 T8737 mark as,judged
R5575 T8879 T8880 compound Figure,6H
R5576 T8880 T8878 pobj 6H,in
R5577 T8881 T8877 punct ", ",displayed
R5578 T8737 T8717 advcl judged,took
R5579 T8882 T8883 compound hair,buds
R5580 T8883 T8877 nsubj buds,displayed
R5581 T8884 T8883 prep in,buds
R5582 T8738 T8737 prep by,judged
R5583 T8885 T8886 compound TGF,β2
R5584 T8886 T8888 npadvmod β2,null
R5585 T8887 T8886 punct -,β2
R5586 T8739 T8738 pobj immunofluorescence,by
R5587 T8888 T8889 amod null,skin
R5588 T8889 T8884 pobj skin,in
R5589 T8740 T8739 prep with,immunofluorescence
R5590 T8890 T8891 amod elevated,staining
R5591 T8891 T8877 dobj staining,displayed
R5592 T8892 T8891 compound immunofluorescence,staining
R5593 T8741 T8740 pobj antibodies,with
R5594 T8893 T8877 advcl relative,displayed
R5595 T8894 T8893 prep to,relative
R5596 T8895 T8896 poss their,counterparts
R5597 T8742 T8741 prep against,antibodies
R5598 T8896 T8894 pobj counterparts,to
R5599 T8897 T8896 compound WT,counterparts
R5600 T8898 T8877 punct .,displayed
R5601 T8743 T8744 compound β4,integrin
R5602 T8900 T8901 advmod Previously,demonstrated
R5603 T8744 T8742 pobj integrin,against
R5604 T8902 T8901 nsubj we,demonstrated
R5605 T8903 T8904 mark that,required
R5606 T8745 T8744 punct ", ",integrin
R5607 T8904 T8901 ccomp required,demonstrated
R5608 T8905 T8906 det the,action
R5609 T8906 T8904 nsubjpass action,required
R5610 T8907 T8906 amod concerted,action
R5611 T8908 T8906 prep of,action
R5612 T8746 T8747 det an,component
R5613 T8909 T8910 det the,signals
R5614 T8910 T8908 pobj signals,of
R5615 T8911 T8910 amod extracellular,signals
R5616 T8747 T8744 appos component,integrin
R5617 T8912 T8910 appos Wnt,signals
R5618 T8913 T8912 cc and,Wnt
R5619 T8914 T8912 conj noggin,Wnt
R5620 T8748 T8747 amod integral,component
R5621 T8915 T8904 auxpass are,required
R5622 T8916 T8917 mark for,repress
R5623 T8749 T8747 prep of,component
R5624 T8750 T8751 npadvmod keratinocyte,mediated
R5625 T8917 T8904 advcl repress,required
R5626 T8918 T8919 det the,generation
R5627 T8751 T8753 amod mediated,adhesion
R5628 T8919 T8917 nsubj generation,repress
R5629 T8920 T8919 prep of,generation
R5630 T8921 T8922 det a,complex
R5631 T8752 T8751 punct -,mediated
R5632 T8922 T8920 pobj complex,of
R5633 T8923 T8922 nmod LEF,complex
R5634 T8924 T8923 punct -,LEF
R5635 T8753 T8749 pobj adhesion,of
R5636 T8925 T8923 nummod 1,LEF
R5637 T8926 T8923 punct /,LEF
R5638 T8927 T8928 compound β,catenin
R5639 T8754 T8753 prep to,adhesion
R5640 T8928 T8923 appos catenin,LEF
R5641 T8929 T8928 punct -,catenin
R5642 T8755 T8756 poss its,membrane
R5643 T8930 T8922 compound transcription,complex
R5644 T8931 T8917 aux to,repress
R5645 T8932 T8933 compound E,cadherin
R5646 T8756 T8754 pobj membrane,to
R5647 T8933 T8935 compound cadherin,transcription
R5648 T8934 T8933 punct -,cadherin
R5649 T8757 T8756 amod underlying,membrane
R5650 T8935 T8917 dobj transcription,repress
R5651 T8936 T8917 prep at,repress
R5652 T8937 T8938 det the,onset
R5653 T8758 T8756 compound basement,membrane
R5654 T8938 T8936 pobj onset,at
R5655 T8939 T8938 prep of,onset
R5656 T8759 T8760 punct (,6F
R5657 T8940 T8941 compound hair,fate
R5658 T8941 T8942 compound fate,specification
R5659 T8942 T8939 pobj specification,of
R5660 T8943 T8901 punct .,demonstrated
R5661 T8760 T8717 parataxis 6F,took
R5662 T8945 T8946 mark As,shown
R5663 T8946 T8947 advcl shown,exhibited
R5664 T8948 T8946 prep in,shown
R5665 T8761 T8760 compound Figure,6F
R5666 T8949 T8950 compound Figure,6I
R5667 T8950 T8948 pobj 6I,in
R5668 T8762 T8760 punct ),6F
R5669 T8951 T8950 cc and,6I
R5670 T8952 T8950 conj 6J,6I
R5671 T8953 T8947 punct ", ",exhibited
R5672 T8763 T8717 punct .,took
R5673 T8954 T8955 preconj both,WT
R5674 T8955 T8956 npadvmod WT,null
R5675 T8765 T8766 prep In,appeared
R5676 T8956 T8961 amod null,buds
R5677 T8957 T8955 cc and,WT
R5678 T8958 T8959 compound TGF,β2
R5679 T8959 T8955 conj β2,WT
R5680 T8960 T8959 punct -,β2
R5681 T8767 T8765 pobj contrast,In
R5682 T8961 T8947 nsubj buds,exhibited
R5683 T8962 T8963 amod nuclear,localization
R5684 T8963 T8947 dobj localization,exhibited
R5685 T8964 T8963 nmod LEF,localization
R5686 T8768 T8766 punct ", ",appeared
R5687 T8965 T8964 punct -,LEF
R5688 T8966 T8964 nummod 1,LEF
R5689 T8967 T8964 cc and,LEF
R5690 T8769 T8770 compound TGF,β2
R5691 T8968 T8969 compound β,catenin
R5692 T8969 T8964 conj catenin,LEF
R5693 T8970 T8969 punct -,catenin
R5694 T8982 T8947 punct .,exhibited
R5695 T8971 T8947 punct ", ",exhibited
R5696 T8972 T8947 npadvmod signs,exhibited
R5697 T8973 T8974 mark that,was
R5698 T8974 T8972 acl was,signs
R5699 T8975 T8976 det the,pathway
R5700 T8976 T8974 nsubj pathway,was
R5701 T8977 T8978 compound Wnt,noggin
R5702 T8984 T8985 det These,data
R5703 T8978 T8976 compound noggin,pathway
R5704 T8979 T8978 punct -,noggin
R5705 T8980 T8976 compound signaling,pathway
R5706 T8981 T8974 acomp intact,was
R5707 T8985 T8986 nsubj data,suggest
R5708 T8987 T8988 mark that,functions
R5709 T8988 T8986 ccomp functions,suggest
R5710 T8989 T8988 prep during,functions
R5711 T8990 T8991 compound hair,follicle
R5712 T8991 T8992 compound follicle,morphogenesis
R5713 T8992 T8989 pobj morphogenesis,during
R5714 T8993 T8988 punct ", ",functions
R5715 T8994 T8995 compound TGF,β2
R5716 T8995 T8988 nsubj β2,functions
R5717 T8996 T8995 punct -,β2
R5718 T8997 T8988 advmod subsequently,functions
R5719 T8998 T8997 prep to,subsequently
R5720 T8999 T9000 compound Wnt,noggin
R5721 T9000 T9002 npadvmod noggin,mediated
R5722 T9001 T9000 punct /,noggin
R5723 T9002 T9004 amod mediated,determination
R5724 T9003 T9002 punct -,mediated
R5725 T9004 T8998 pobj determination,to
R5726 T9005 T9004 prep of,determination
R5727 T9006 T9007 compound hair,fate
R5728 T9007 T9005 pobj fate,of
R5772 T9102 T9103 prep During,govern
R5773 T9104 T9105 compound budding,morphogenesis
R5774 T9105 T9102 pobj morphogenesis,During
R5775 T9106 T9103 punct ", ",govern
R5776 T9107 T9108 amod intersecting,networks
R5777 T9108 T9103 nsubj networks,govern
R5778 T9109 T9108 compound signaling,networks
R5779 T9110 T9108 prep from,networks
R5780 T9111 T9112 det the,epithelium
R5781 T9112 T9110 pobj epithelium,from
R5782 T9113 T9112 cc and,epithelium
R5783 T9114 T9112 conj mesenchyme,epithelium
R5784 T9115 T9116 amod transcriptional,programs
R5785 T9116 T9103 dobj programs,govern
R5786 T9117 T9115 punct ", ",transcriptional
R5787 T9118 T9115 conj adhesive,transcriptional
R5788 T9119 T9118 punct ", ",adhesive
R5789 T9120 T9118 conj polarity,adhesive
R5790 T9121 T9120 punct ", ",polarity
R5791 T9122 T9120 cc and,polarity
R5792 T9123 T9120 conj motility,polarity
R5793 T9124 T9103 prep in,govern
R5794 T9125 T9126 det these,groups
R5795 T9126 T9124 pobj groups,in
R5796 T9127 T9126 amod select,groups
R5797 T9128 T9126 prep of,groups
R5798 T9129 T9128 pobj cells,of
R5799 T9130 T9103 punct .,govern
R5800 T9132 T9133 det The,changes
R5801 T9133 T9138 nsubj changes,form
R5802 T9134 T9133 amod dynamic,changes
R5803 T9135 T9133 amod nuclear,changes
R5804 T9136 T9135 cc and,nuclear
R5805 T9137 T9135 conj cytosolic,nuclear
R5806 T9139 T9140 dep that,take
R5807 T9140 T9133 relcl take,changes
R5808 T9141 T9140 dobj place,take
R5809 T9142 T9140 prep during,take
R5810 T9143 T9144 det this,time
R5811 T9144 T9142 pobj time,during
R5812 T9145 T9146 det the,cornerstone
R5813 T9146 T9138 dobj cornerstone,form
R5814 T9147 T9146 prep for,cornerstone
R5815 T9148 T9149 compound organ,morphogenesis
R5816 T9149 T9147 pobj morphogenesis,for
R5817 T9150 T9138 punct .,form
R5818 T9152 T9153 nummod Two,challenges
R5819 T9153 T9155 nsubj challenges,are
R5820 T9154 T9153 amod major,challenges
R5821 T9156 T9153 prep in,challenges
R5822 T9157 T9156 pcomp understanding,in
R5823 T9158 T9159 det the,mechanisms
R5824 T9159 T9157 dobj mechanisms,understanding
R5825 T9160 T9159 acl underlying,mechanisms
R5826 T9161 T9162 det a,process
R5827 T9162 T9160 dobj process,underlying
R5828 T9163 T9162 amod particular,process
R5829 T9164 T9162 compound budding,process
R5830 T9165 T9166 aux to,order
R5831 T9166 T9155 xcomp order,are
R5832 T9167 T9168 det the,sequence
R5833 T9168 T9166 dobj sequence,order
R5834 T9169 T9168 amod temporal,sequence
R5835 T9170 T9168 prep of,sequence
R5836 T9171 T9172 amod external,cues
R5837 T9172 T9170 pobj cues,of
R5838 T9173 T9172 acl involved,cues
R5839 T9174 T9166 cc and,order
R5840 T9175 T9176 advmod then,dissect
R5841 T9176 T9166 conj dissect,order
R5842 T9177 T9176 aux to,dissect
R5843 T9178 T9179 advmod how,translate
R5844 T9179 T9176 ccomp translate,dissect
R5845 T9180 T9181 det the,cells
R5846 T9181 T9179 nsubj cells,translate
R5847 T9182 T9181 prep of,cells
R5848 T9183 T9184 det the,bud
R5849 T9184 T9182 pobj bud,of
R5850 T9185 T9184 amod developing,bud
R5851 T9186 T9187 det these,signals
R5852 T9187 T9179 dobj signals,translate
R5853 T9188 T9179 prep into,translate
R5854 T9189 T9190 det the,events
R5855 T9190 T9188 pobj events,into
R5856 T9191 T9190 amod downstream,events
R5857 T9192 T9190 prep of,events
R5858 T9193 T9194 amod cellular,remodeling
R5859 T9194 T9192 pobj remodeling,of
R5860 T9195 T9194 punct ", ",remodeling
R5861 T9196 T9194 conj proliferation,remodeling
R5862 T9197 T9196 punct ", ",proliferation
R5863 T9198 T9196 cc and,proliferation
R5864 T9199 T9196 conj differentiation,proliferation
R5865 T9200 T9155 punct .,are
R5866 T9202 T9203 poss Our,studies
R5867 T9203 T9204 nsubj studies,provide
R5868 T9205 T9203 advmod here,studies
R5869 T9206 T9207 det some,insights
R5870 T9207 T9204 dobj insights,provide
R5871 T9208 T9207 prep into,insights
R5872 T9209 T9210 advmod how,orchestrated
R5873 T9210 T9208 pcomp orchestrated,into
R5874 T9211 T9212 det these,events
R5875 T9212 T9210 nsubjpass events,orchestrated
R5876 T9213 T9210 auxpass are,orchestrated
R5877 T9214 T9210 prep during,orchestrated
R5878 T9215 T9216 compound hair,bud
R5879 T9216 T9217 compound bud,formation
R5880 T9217 T9214 pobj formation,during
R5881 T9218 T9210 prep in,orchestrated
R5882 T9219 T9220 amod developing,skin
R5883 T9220 T9218 pobj skin,in
R5884 T9221 T9204 punct .,provide
R5895 T9654 T9653 prep during,Signaling
R5896 T9655 T9656 amod Early,Morphogenesis
R5897 T9656 T9654 pobj Morphogenesis,during
R5898 T9657 T9658 compound Hair,Follicle
R5899 T9658 T9656 compound Follicle,Morphogenesis
R5900 T9660 T9661 amod Recent,studies
R5901 T9661 T9662 nsubj studies,suggest
R5902 T9663 T9661 prep on,studies
R5903 T9664 T9665 compound hair,bud
R5904 T9665 T9666 compound bud,morphogenesis
R5905 T9666 T9663 pobj morphogenesis,on
R5906 T9667 T9668 mark that,converge
R5907 T9668 T9662 ccomp converge,suggest
R5908 T9669 T9670 compound Wnt,signals
R5909 T9670 T9668 nsubj signals,converge
R5910 T9671 T9672 advmod likely,from
R5911 T9672 T9670 prep from,signals
R5912 T9673 T9674 det the,epithelium
R5913 T9674 T9672 pobj epithelium,from
R5914 T9675 T9670 cc and,signals
R5915 T9676 T9677 nmod BMP,signals
R5916 T9677 T9670 conj signals,signals
R5917 T9678 T9677 amod inhibitory,signals
R5918 T9679 T9677 prep from,signals
R5919 T9680 T9681 det the,mesenchyme
R5920 T9681 T9679 pobj mesenchyme,from
R5921 T9682 T9681 amod underlying,mesenchyme
R5922 T9683 T9684 aux to,produce
R5923 T9684 T9668 advcl produce,converge
R5924 T9685 T9686 det an,complex
R5925 T9686 T9684 dobj complex,produce
R5926 T9687 T9686 amod active,complex
R5927 T9688 T9689 compound transcription,factor
R5928 T9689 T9686 compound factor,complex
R5929 T9690 T9686 acl involving,complex
R5930 T9691 T9692 compound β,catenin
R5931 T9692 T9690 dobj catenin,involving
R5932 T9693 T9692 punct -,catenin
R5933 T9694 T9692 cc and,catenin
R5934 T9695 T9692 conj LEF,catenin
R5935 T9696 T9695 punct -,LEF
R5936 T9697 T9695 nummod 1,LEF
R5937 T9698 T9686 punct ", ",complex
R5938 T9699 T9700 dep which,plays
R5939 T9700 T9686 relcl plays,complex
R5940 T9701 T9700 prep in,plays
R5941 T9702 T9701 pobj turn,in
R5942 T9703 T9704 det a,role
R5943 T9704 T9700 dobj role,plays
R5944 T9705 T9704 amod key,role
R5945 T9706 T9700 prep in,plays
R5946 T9707 T9706 pcomp specifying,in
R5947 T9708 T9709 det the,fate
R5948 T9709 T9707 dobj fate,specifying
R5949 T9710 T9711 compound hair,follicle
R5950 T9711 T9709 compound follicle,fate
R5951 T9712 T9713 punct [,37
R5952 T9713 T9684 parataxis 37,produce
R5953 T9714 T9713 nummod 4,37
R5954 T9715 T9713 punct ",",37
R5955 T9716 T9713 nummod 29,37
R5956 T9717 T9713 punct ",",37
R5957 T9718 T9713 nummod 30,37
R5958 T9719 T9713 punct ",",37
R5959 T9720 T9713 nummod 36,37
R5960 T9721 T9713 punct ",",37
R5961 T9722 T9713 punct ],37
R5962 T9723 T9662 punct .,suggest
R5963 T9725 T9726 amod Sonic,hedgehog
R5964 T9726 T9727 nsubj hedgehog,play
R5965 T9728 T9726 punct (,hedgehog
R5966 T9729 T9726 appos Shh,hedgehog
R5967 T9730 T9726 punct ),hedgehog
R5968 T9731 T9726 cc and,hedgehog
R5969 T9732 T9733 compound TGF,β2
R5970 T9733 T9735 compound β2,signaling
R5971 T9734 T9733 punct -,β2
R5972 T9735 T9726 conj signaling,hedgehog
R5973 T9736 T9727 advmod also,play
R5974 T9737 T9738 amod essential,roles
R5975 T9738 T9727 dobj roles,play
R5976 T9739 T9727 prep in,play
R5977 T9740 T9741 compound follicle,morphogenesis
R5978 T9741 T9739 pobj morphogenesis,in
R5979 T9742 T9727 punct ", ",play
R5980 T9743 T9727 cc but,play
R5981 T9744 T9745 prep in,form
R5982 T9745 T9727 conj form,play
R5983 T9746 T9744 pobj contrast,in
R5984 T9747 T9746 prep to,contrast
R5985 T9748 T9749 compound β,catenin
R5986 T9749 T9751 npadvmod catenin,null
R5987 T9750 T9749 punct -,catenin
R5988 T9751 T9752 amod null,skin
R5989 T9752 T9747 pobj skin,to
R5990 T9753 T9752 punct ", ",skin
R5991 T9754 T9755 prep in,are
R5992 T9755 T9752 relcl are,skin
R5993 T9756 T9754 pobj which,in
R5994 T9757 T9758 compound follicle,invaginations
R5995 T9758 T9755 nsubj invaginations,are
R5996 T9759 T9755 acomp absent,are
R5997 T9760 T9761 punct [,30
R5998 T9761 T9747 parataxis 30,to
R5999 T9762 T9761 punct ],30
R6000 T9763 T9745 punct ", ",form
R6001 T9764 T9765 det some,buds
R6002 T9765 T9745 nsubj buds,form
R6003 T9766 T9765 compound hair,buds
R6004 T9767 T9745 advmod still,form
R6005 T9768 T9745 prep in,form
R6006 T9769 T9770 det the,absence
R6007 T9770 T9768 pobj absence,in
R6008 T9771 T9770 prep of,absence
R6009 T9772 T9771 pobj LEF,of
R6010 T9773 T9772 punct -,LEF
R6011 T9774 T9772 nummod 1,LEF
R6012 T9775 T9772 punct ", ",LEF
R6013 T9776 T9772 conj Shh,LEF
R6014 T9777 T9776 punct ", ",Shh
R6015 T9778 T9776 cc or,Shh
R6016 T9779 T9780 compound TGF,β2
R6017 T9780 T9776 conj β2,Shh
R6018 T9781 T9780 punct -,β2
R6019 T9782 T9783 punct [,38
R6020 T9783 T9745 parataxis 38,form
R6021 T9784 T9783 nummod 32,38
R6022 T9785 T9783 punct ",",38
R6023 T9786 T9783 punct ],38
R6024 T9787 T9745 punct .,form
R6025 T9789 T9790 nsubj These,reflect
R6026 T9791 T9790 advmod likely,reflect
R6027 T9792 T9793 det the,wave
R6028 T9793 T9790 dobj wave,reflect
R6029 T9794 T9793 amod first,wave
R6030 T9795 T9793 prep of,wave
R6031 T9796 T9797 nmod follicle,morphogenesis
R6032 T9797 T9795 pobj morphogenesis,of
R6033 T9798 T9799 punct (,hair
R6034 T9799 T9797 parataxis hair,morphogenesis
R6035 T9800 T9799 advmod i.e.,hair
R6036 T9801 T9799 punct ", ",hair
R6037 T9802 T9799 compound guard,hair
R6038 T9803 T9799 punct ),hair
R6039 T9804 T9793 punct ", ",wave
R6040 T9805 T9806 dep which,accounts
R6041 T9806 T9793 relcl accounts,wave
R6042 T9807 T9806 prep for,accounts
R6043 T9808 T9809 det a,number
R6044 T9809 T9807 pobj number,for
R6045 T9810 T9809 amod small,number
R6046 T9811 T9809 punct (,number
R6047 T9812 T9813 amod fewer,5
R6048 T9813 T9815 nummod 5,%
R6049 T9814 T9813 quantmod than,5
R6050 T9815 T9809 appos %,number
R6051 T9816 T9809 punct ),number
R6052 T9817 T9809 prep of,number
R6053 T9818 T9817 pobj hairs,of
R6054 T9819 T9806 cc and,accounts
R6055 T9820 T9806 conj is,accounts
R6056 T9821 T9820 prep under,is
R6057 T9822 T9823 amod distinct,control
R6058 T9823 T9821 pobj control,under
R6059 T9824 T9823 amod regulatory,control
R6060 T9825 T9790 punct .,reflect
R6061 T9827 T9828 compound Guard,hairs
R6062 T9828 T9829 nsubj hairs,form
R6063 T9830 T9829 prep in,form
R6064 T9831 T9832 det the,absence
R6065 T9832 T9830 pobj absence,in
R6066 T9833 T9832 prep of,absence
R6067 T9834 T9833 pobj LEF,of
R6068 T9835 T9834 punct -,LEF
R6069 T9836 T9834 nummod 1,LEF
R6070 T9837 T9834 cc and,LEF
R6071 T9838 T9839 compound TGF,β2
R6072 T9839 T9834 conj β2,LEF
R6073 T9840 T9839 punct -,β2
R6074 T9841 T9829 punct ", ",form
R6075 T9842 T9829 cc and,form
R6076 T9843 T9844 nsubj we,found
R6077 T9844 T9829 conj found,form
R6078 T9845 T9844 aux have,found
R6079 T9846 T9847 mark that,fail
R6080 T9847 T9844 ccomp fail,found
R6081 T9848 T9847 nsubj they,fail
R6082 T9849 T9847 advmod also,fail
R6083 T9850 T9851 aux to,express
R6084 T9851 T9847 xcomp express,fail
R6085 T9852 T9851 dobj Snail,express
R6086 T9853 T9847 prep at,fail
R6087 T9854 T9855 det the,stage
R6088 T9855 T9853 pobj stage,at
R6089 T9856 T9855 compound budding,stage
R6090 T9857 T9855 prep of,stage
R6091 T9858 T9857 pobj development,of
R6092 T9859 T9860 punct (,data
R6093 T9860 T9844 meta data,found
R6094 T9861 T9860 amod unpublished,data
R6095 T9862 T9860 punct ),data
R6096 T9863 T9844 punct .,found
R6097 T9865 T9866 advmod How,regulated
R6098 T9866 T9871 csubj regulated,remains
R6099 T9867 T9868 compound E,cadherin
R6100 T9868 T9866 nsubjpass cadherin,regulated
R6101 T9869 T9868 punct -,cadherin
R6102 T9870 T9866 auxpass is,regulated
R6103 T9872 T9866 prep in,regulated
R6104 T9873 T9874 compound guard,hairs
R6105 T9874 T9872 pobj hairs,in
R6106 T9875 T9876 aux to,determined
R6107 T9876 T9871 xcomp determined,remains
R6108 T9877 T9876 auxpass be,determined
R6109 T9878 T9871 punct .,remains
R6110 T9880 T9881 amod Several,candidates
R6111 T9881 T9882 nsubj candidates,include
R6112 T9883 T9884 amod other,members
R6113 T9884 T9882 dobj members,include
R6114 T9885 T9884 compound Snail,members
R6115 T9886 T9884 compound family,members
R6116 T9887 T9888 amod such,as
R6117 T9888 T9884 prep as,members
R6118 T9889 T9888 pobj Slug,as
R6119 T9890 T9889 cc or,Slug
R6120 T9891 T9889 conj twist,Slug
R6121 T9892 T9884 punct ", ",members
R6122 T9893 T9884 cc or,members
R6123 T9894 T9895 advmod alternatively,factors
R6124 T9895 T9884 conj factors,members
R6125 T9896 T9895 punct ", ",factors
R6126 T9897 T9895 compound transcription,factors
R6127 T9898 T9895 acl involving,factors
R6128 T9899 T9900 compound β,catenin
R6129 T9900 T9898 dobj catenin,involving
R6130 T9901 T9900 punct -,catenin
R6131 T9902 T9900 cc and,catenin
R6132 T9903 T9904 det a,member
R6133 T9904 T9900 conj member,catenin
R6134 T9905 T9904 amod different,member
R6135 T9906 T9904 prep of,member
R6136 T9907 T9908 det the,family
R6137 T9908 T9906 pobj family,of
R6138 T9909 T9910 nmod LEF,box
R6139 T9910 T9908 nmod box,family
R6140 T9911 T9909 punct -,LEF
R6141 T9912 T9909 nummod 1,LEF
R6142 T9913 T9909 punct /,LEF
R6143 T9914 T9909 appos TCF,LEF
R6144 T9915 T9909 punct /,LEF
R6145 T9916 T9917 compound Sry,type
R6146 T9917 T9909 appos type,LEF
R6147 T9918 T9917 punct -,type
R6148 T9919 T9910 nmod HMG,box
R6149 T9920 T9921 punct (,known
R6150 T9921 T9910 parataxis known,box
R6151 T9922 T9921 advmod commonly,known
R6152 T9923 T9921 prep as,known
R6153 T9924 T9923 pobj SOX,as
R6154 T9925 T9921 punct ),known
R6155 T9926 T9927 punct [,40
R6156 T9927 T9882 parataxis 40,include
R6157 T9928 T9927 nummod 39,40
R6158 T9929 T9927 punct ",",40
R6159 T9930 T9927 punct ],40
R6160 T9931 T9882 punct .,include
R6161 T9933 T9934 amod Further,investigation
R6162 T9934 T9935 nsubjpass investigation,required
R6163 T9936 T9935 aux will,required
R6164 T9937 T9935 auxpass be,required
R6165 T9938 T9939 aux to,determine
R6166 T9939 T9935 advcl determine,required
R6167 T9940 T9941 mark whether,is
R6168 T9941 T9939 ccomp is,determine
R6169 T9942 T9943 det the,pathway
R6170 T9943 T9941 nsubj pathway,is
R6171 T9944 T9943 compound signaling,pathway
R6172 T9945 T9946 nsubj we,elucidated
R6173 T9946 T9943 advcl elucidated,pathway
R6174 T9947 T9946 aux have,elucidated
R6175 T9948 T9946 advmod here,elucidated
R6176 T9949 T9950 det a,theme
R6177 T9950 T9941 attr theme,is
R6178 T9951 T9950 prep with,theme
R6179 T9952 T9953 amod multiple,variations
R6180 T9953 T9951 pobj variations,with
R6181 T9954 T9935 punct .,required
R6182 T9956 T9957 compound TGF,βs
R6183 T9957 T9959 nsubjpass βs,known
R6184 T9958 T9957 punct -,βs
R6185 T9960 T9959 auxpass are,known
R6186 T9961 T9962 aux to,promote
R6187 T9962 T9959 xcomp promote,known
R6188 T9963 T9962 dobj withdrawal,promote
R6189 T9964 T9963 prep of,withdrawal
R6190 T9965 T9964 pobj keratinocytes,of
R6191 T9966 T9963 prep from,withdrawal
R6192 T9967 T9968 det the,cycle
R6193 T9968 T9966 pobj cycle,from
R6194 T9969 T9968 compound cell,cycle
R6195 T9970 T9971 punct [,41
R6196 T9971 T9959 parataxis 41,known
R6197 T9972 T9971 punct ],41
R6198 T9973 T9959 punct .,known
R6199 T9975 T9976 advmod Hence,focused
R6200 T9977 T9976 punct ", ",focused
R6201 T9978 T9979 advmod when,detected
R6202 T9979 T9976 advcl detected,focused
R6203 T9980 T9981 compound TGF,β2
R6204 T9981 T9983 compound β2,protein
R6205 T9982 T9981 punct -,β2
R6206 T9983 T9979 nsubjpass protein,detected
R6207 T9984 T9979 auxpass was,detected
R6208 T9985 T9979 prep at,detected
R6209 T9986 T9987 det the,transition
R6210 T9987 T9985 pobj transition,at
R6211 T9988 T9987 prep between,transition
R6212 T9989 T9990 det the,phases
R6213 T9990 T9988 pobj phases,between
R6214 T9991 T9992 npadvmod growing,destructive
R6215 T9992 T9990 amod destructive,phases
R6216 T9993 T9992 cc and,destructive
R6217 T9994 T9990 prep of,phases
R6218 T9995 T9996 det the,cycle
R6219 T9996 T9994 pobj cycle,of
R6220 T9997 T9996 amod adult,cycle
R6221 T9998 T9996 compound hair,cycle
R6222 T9999 T9976 punct ", ",focused
R6223 T10000 T9976 nsubj research,focused
R6224 T10001 T9976 advmod initially,focused
R6225 T10002 T10001 cc and,initially
R6226 T10003 T10001 conj naturally,initially
R6227 T10004 T9976 prep on,focused
R6228 T10005 T10006 det a,role
R6229 T10006 T10004 pobj role,on
R6230 T10007 T10006 prep for,role
R6231 T10008 T10009 det this,member
R6232 T10009 T10007 pobj member,for
R6233 T10010 T10009 compound family,member
R6234 T10011 T10006 prep in,role
R6235 T10012 T10011 pobj cessation,in
R6236 T10013 T10012 prep of,cessation
R6237 T10014 T10013 pobj growth,of
R6238 T10015 T10012 cc and,cessation
R6239 T10016 T10015 punct /,and
R6240 T10017 T10015 cc or,and
R6241 T10018 T10012 conj triggering,cessation
R6242 T10019 T10018 dobj apoptosis,triggering
R6243 T10020 T10021 punct (,42
R6244 T10021 T9976 parataxis 42,focused
R6245 T10022 T10021 punct [,42
R6246 T10023 T10021 punct ],42
R6247 T10024 T10021 cc and,42
R6248 T10025 T10021 conj references,42
R6249 T10026 T10025 advmod therein,references
R6250 T10027 T10021 punct ),42
R6251 T10028 T9976 punct .,focused
R6252 T10030 T10031 advmod However,displays
R6253 T10032 T10031 punct ", ",displays
R6254 T10033 T10031 prep in,displays
R6255 T10034 T10033 pobj contrast,in
R6256 T10035 T10034 prep to,contrast
R6257 T10036 T10037 compound TGF,β1
R6258 T10037 T10039 npadvmod β1,null
R6259 T10038 T10037 punct -,β1
R6260 T10039 T10041 amod null,skin
R6261 T10040 T10039 punct -,null
R6262 T10041 T10035 pobj skin,to
R6263 T10042 T10041 punct ", ",skin
R6264 T10043 T10044 dep which,exhibits
R6265 T10044 T10041 relcl exhibits,skin
R6266 T10045 T10046 det an,phase
R6267 T10046 T10044 dobj phase,exhibits
R6268 T10047 T10046 amod extended,phase
R6269 T10048 T10046 compound growing,phase
R6270 T10049 T10046 prep of,phase
R6271 T10050 T10051 amod postnatal,follicles
R6272 T10051 T10049 pobj follicles,of
R6273 T10052 T10051 compound hair,follicles
R6274 T10053 T10031 punct ", ",displays
R6275 T10054 T10055 compound TGF,β2
R6276 T10055 T10057 npadvmod β2,null
R6277 T10056 T10055 punct -,β2
R6278 T10057 T10059 amod null,skin
R6279 T10058 T10057 punct -,null
R6280 T10059 T10031 nsubj skin,displays
R6281 T10060 T10061 det an,block
R6282 T10061 T10031 dobj block,displays
R6283 T10062 T10061 amod embryonic,block
R6284 T10063 T10061 prep in,block
R6285 T10064 T10065 compound follicle,bud
R6286 T10065 T10066 compound bud,progression
R6287 T10066 T10063 pobj progression,in
R6288 T10067 T10068 punct [,32
R6289 T10068 T10031 parataxis 32,displays
R6290 T10069 T10068 punct ],32
R6291 T10070 T10031 punct .,displays
R6292 T10072 T10073 mark Although,is
R6293 T10073 T10076 advcl is,appear
R6294 T10074 T10075 det this,phenotype
R6295 T10075 T10073 nsubj phenotype,is
R6296 T10077 T10073 acomp consistent,is
R6297 T10078 T10077 prep with,consistent
R6298 T10079 T10080 compound TGF,β2
R6299 T10080 T10082 poss β2,patterns
R6300 T10081 T10080 punct -,β2
R6301 T10082 T10078 pobj patterns,with
R6302 T10083 T10080 case 's,β2
R6303 T10084 T10082 amod embryonic,patterns
R6304 T10085 T10082 compound expression,patterns
R6305 T10086 T10087 punct [,33
R6306 T10087 T10073 parataxis 33,is
R6307 T10088 T10087 punct ],33
R6308 T10089 T10076 punct ", ",appear
R6309 T10090 T10091 quantmod about,50
R6310 T10091 T10092 nummod 50,%
R6311 T10092 T10076 nsubj %,appear
R6312 T10093 T10092 prep of,%
R6313 T10094 T10095 compound TGF,β2
R6314 T10095 T10097 npadvmod β2,null
R6315 T10096 T10095 punct -,β2
R6316 T10097 T10098 amod null,buds
R6317 T10098 T10093 pobj buds,of
R6318 T10099 T10076 oprd unable,appear
R6319 T10100 T10101 aux to,progress
R6320 T10101 T10099 xcomp progress,unable
R6321 T10102 T10101 prep to,progress
R6322 T10103 T10104 det the,phase
R6323 T10104 T10102 pobj phase,to
R6324 T10105 T10106 amod down,growth
R6325 T10106 T10104 compound growth,phase
R6326 T10107 T10106 punct -,growth
R6327 T10108 T10099 punct ", ",unable
R6328 T10109 T10110 det a,feature
R6329 T10110 T10099 npadvmod feature,unable
R6330 T10111 T10112 dep that,explained
R6331 T10112 T10110 relcl explained,feature
R6332 T10113 T10112 aux can,explained
R6333 T10114 T10112 neg not,explained
R6334 T10115 T10112 auxpass be,explained
R6335 T10116 T10112 advmod readily,explained
R6336 T10117 T10112 prep on,explained
R6337 T10118 T10119 det the,basis
R6338 T10119 T10117 pobj basis,on
R6339 T10120 T10119 prep of,basis
R6340 T10121 T10122 advmod previously,established
R6341 T10122 T10123 amod established,effects
R6342 T10123 T10120 pobj effects,of
R6343 T10124 T10123 prep of,effects
R6344 T10125 T10126 compound TGF,βs
R6345 T10126 T10124 pobj βs,of
R6346 T10127 T10126 punct -,βs
R6347 T10128 T10076 punct .,appear
R6348 T10130 T10131 poss Our,finding
R6349 T10131 T10132 nsubj finding,lends
R6350 T10133 T10134 mark that,is
R6351 T10134 T10131 acl is,finding
R6352 T10135 T10136 compound TGF,β2
R6353 T10136 T10134 nsubj β2,is
R6354 T10137 T10136 punct -,β2
R6355 T10138 T10134 advmod upstream,is
R6356 T10139 T10138 prep from,upstream
R6357 T10140 T10141 compound Ki67,expression
R6358 T10141 T10139 pobj expression,from
R6359 T10142 T10141 cc and,expression
R6360 T10143 T10144 compound MAPK,activation
R6361 T10144 T10141 conj activation,expression
R6362 T10145 T10146 amod further,support
R6363 T10146 T10132 dobj support,lends
R6364 T10147 T10132 dative to,lends
R6365 T10148 T10149 det the,notion
R6366 T10149 T10147 pobj notion,to
R6367 T10150 T10151 mark that,react
R6368 T10151 T10149 acl react,notion
R6369 T10152 T10153 compound hair,follicle
R6370 T10153 T10154 compound follicle,keratinocytes
R6371 T10154 T10151 nsubj keratinocytes,react
R6372 T10155 T10154 prep at,keratinocytes
R6373 T10156 T10157 det this,stage
R6374 T10157 T10155 pobj stage,at
R6375 T10158 T10157 amod early,stage
R6376 T10159 T10157 prep of,stage
R6377 T10160 T10159 pobj development,of
R6378 T10161 T10151 prep to,react
R6379 T10162 T10163 compound TGF,β2
R6380 T10163 T10165 compound β2,signaling
R6381 T10164 T10163 punct -,β2
R6382 T10165 T10161 pobj signaling,to
R6383 T10166 T10151 prep in,react
R6384 T10167 T10168 det a,fashion
R6385 T10168 T10166 pobj fashion,in
R6386 T10169 T10168 amod opposite,fashion
R6387 T10170 T10169 prep to,opposite
R6388 T10171 T10170 pobj that,to
R6389 T10172 T10173 advmod typically,expected
R6390 T10173 T10171 acl expected,that
R6391 T10174 T10173 prep for,expected
R6392 T10175 T10176 compound TGF,β
R6393 T10176 T10178 compound β,factors
R6394 T10177 T10176 punct -,β
R6395 T10178 T10174 pobj factors,for
R6396 T10179 T10132 punct .,lends
R6397 T10181 T10182 nsubj This,said
R6398 T10182 T10183 advcl said,appeared
R6399 T10184 T10183 punct ", ",appeared
R6400 T10185 T10183 prep based,appeared
R6401 T10186 T10185 prep upon,based
R6402 T10187 T10188 compound pSMAD2,immunohistochemistry
R6403 T10188 T10186 pobj immunohistochemistry,upon
R6404 T10189 T10183 punct ", ",appeared
R6405 T10190 T10191 det the,steps
R6406 T10191 T10183 nsubj steps,appeared
R6407 T10192 T10191 amod immediate,steps
R6408 T10193 T10191 prep of,steps
R6409 T10194 T10195 amod downstream,signaling
R6410 T10195 T10193 pobj signaling,of
R6411 T10196 T10197 aux to,be
R6412 T10197 T10183 xcomp be,appeared
R6413 T10198 T10197 acomp intact,be
R6414 T10199 T10183 punct .,appeared
R6415 T10201 T10202 advmod Thus,surmise
R6416 T10203 T10202 punct ", ",surmise
R6417 T10204 T10202 nsubj we,surmise
R6418 T10205 T10206 mark that,is
R6419 T10206 T10202 ccomp is,surmise
R6420 T10207 T10208 det the,outcome
R6421 T10208 T10206 nsubj outcome,is
R6422 T10209 T10208 amod proliferative,outcome
R6423 T10210 T10206 acomp likely,is
R6424 T10211 T10212 aux to,rooted
R6425 T10212 T10210 xcomp rooted,likely
R6426 T10213 T10212 auxpass be,rooted
R6427 T10214 T10212 prep in,rooted
R6428 T10215 T10214 pobj differences,in
R6429 T10216 T10215 prep in,differences
R6430 T10217 T10218 det the,repertoire
R6431 T10218 T10216 pobj repertoire,in
R6432 T10219 T10218 prep of,repertoire
R6433 T10220 T10221 amod activated,genes
R6434 T10221 T10219 pobj genes,of
R6435 T10222 T10221 compound SMAD,genes
R6436 T10223 T10221 compound target,genes
R6437 T10224 T10202 punct .,surmise
R6438 T10226 T10227 prep In,be
R6439 T10228 T10229 det this,regard
R6440 T10229 T10226 pobj regard,In
R6441 T10230 T10227 punct ", ",be
R6442 T10231 T10232 det the,effects
R6443 T10232 T10227 nsubj effects,be
R6444 T10233 T10232 amod positive,effects
R6445 T10234 T10232 prep of,effects
R6446 T10235 T10236 compound TGF,β2
R6447 T10236 T10234 pobj β2,of
R6448 T10237 T10236 punct -,β2
R6449 T10238 T10232 prep on,effects
R6450 T10239 T10238 pobj proliferation,on
R6451 T10240 T10232 prep within,effects
R6452 T10241 T10242 det the,bud
R6453 T10242 T10240 pobj bud,within
R6454 T10243 T10242 compound hair,bud
R6455 T10244 T10227 aux may,be
R6456 T10245 T10246 advmod more,analogous
R6457 T10246 T10227 acomp analogous,be
R6458 T10247 T10246 prep to,analogous
R6459 T10248 T10249 dep what,seen
R6460 T10249 T10247 pobj seen,to
R6461 T10250 T10249 aux has,seen
R6462 T10251 T10249 auxpass been,seen
R6463 T10252 T10249 prep in,seen
R6464 T10253 T10252 pobj progression,in
R6465 T10254 T10253 prep of,progression
R6466 T10255 T10256 amod squamous,cell
R6467 T10256 T10257 compound cell,carcinoma
R6468 T10257 T10254 pobj carcinoma,of
R6469 T10258 T10253 prep to,progression
R6470 T10259 T10260 amod metastatic,carcinoma
R6471 T10260 T10258 pobj carcinoma,to
R6472 T10261 T10262 punct [,43
R6473 T10262 T10249 parataxis 43,seen
R6474 T10263 T10262 punct ],43
R6475 T10264 T10265 advmod rather,than
R6476 T10265 T10249 cc than,seen
R6477 T10266 T10249 conj that,seen
R6478 T10267 T10268 advmod typically,observed
R6479 T10268 T10266 acl observed,that
R6480 T10269 T10268 prep for,observed
R6481 T10270 T10269 pobj keratinocytes,for
R6482 T10271 T10272 punct [,46
R6483 T10272 T10227 parataxis 46,be
R6484 T10273 T10272 nummod 44,46
R6485 T10274 T10272 punct ",",46
R6486 T10275 T10272 nummod 45,46
R6487 T10276 T10272 punct ",",46
R6488 T10277 T10272 punct ],46
R6489 T10278 T10227 punct .,be
R6490 T10280 T10281 det The,identification
R6491 T10281 T10283 nsubj identification,was
R6492 T10282 T10281 amod prior,identification
R6493 T10284 T10281 prep of,identification
R6494 T10285 T10286 det the,gene
R6495 T10286 T10284 pobj gene,of
R6496 T10287 T10286 compound Snail,gene
R6497 T10288 T10281 prep as,identification
R6498 T10289 T10290 det a,target
R6499 T10290 T10288 pobj target,as
R6500 T10291 T10290 amod potential,target
R6501 T10292 T10290 prep of,target
R6502 T10293 T10294 compound TGF,β
R6503 T10294 T10296 compound β,signaling
R6504 T10295 T10294 punct -,β
R6505 T10296 T10292 pobj signaling,of
R6506 T10297 T10298 punct [,15
R6507 T10298 T10281 parataxis 15,identification
R6508 T10299 T10298 punct ],15
R6509 T10300 T10283 acomp intriguing,was
R6510 T10301 T10283 punct ", ",was
R6511 T10302 T10283 prep given,was
R6512 T10303 T10304 det the,wave
R6513 T10304 T10302 pobj wave,given
R6514 T10305 T10304 amod temporal,wave
R6515 T10306 T10304 prep of,wave
R6516 T10307 T10308 compound Snail,expression
R6517 T10308 T10306 pobj expression,of
R6518 T10309 T10308 compound gene,expression
R6519 T10310 T10311 dep that,occurs
R6520 T10311 T10304 relcl occurs,wave
R6521 T10312 T10311 prep in,occurs
R6522 T10313 T10314 det the,bud
R6523 T10314 T10312 pobj bud,in
R6524 T10315 T10314 amod developing,bud
R6525 T10316 T10314 compound hair,bud
R6526 T10317 T10283 punct .,was
R6527 T10319 T10320 det The,correlation
R6528 T10320 T10322 nsubj correlation,fostered
R6529 T10321 T10320 amod additional,correlation
R6530 T10323 T10320 prep between,correlation
R6531 T10324 T10325 amod epithelial,hyperproliferation
R6532 T10325 T10323 pobj hyperproliferation,between
R6533 T10326 T10325 cc and,hyperproliferation
R6534 T10327 T10328 compound Snail,expression
R6535 T10328 T10325 conj expression,hyperproliferation
R6536 T10329 T10328 compound transgene,expression
R6537 T10330 T10322 advmod further,fostered
R6538 T10331 T10332 poss our,interest
R6539 T10332 T10322 dobj interest,fostered
R6540 T10333 T10332 prep in,interest
R6541 T10334 T10335 det a,link
R6542 T10335 T10333 pobj link,in
R6543 T10336 T10335 amod possible,link
R6544 T10337 T10335 prep between,link
R6545 T10338 T10339 compound TGF,β2
R6546 T10339 T10337 pobj β2,between
R6547 T10340 T10339 punct -,β2
R6548 T10341 T10339 cc and,β2
R6549 T10342 T10339 conj Snail,β2
R6550 T10343 T10322 punct .,fostered
R6551 T10345 T10346 poss Our,studies
R6552 T10346 T10348 nsubj studies,demonstrate
R6553 T10347 T10346 amod functional,studies
R6554 T10349 T10350 mark that,abolished
R6555 T10350 T10348 ccomp abolished,demonstrate
R6556 T10351 T10350 prep without,abolished
R6557 T10352 T10353 compound TGF,β2
R6558 T10353 T10351 pobj β2,without
R6559 T10354 T10353 punct -,β2
R6560 T10355 T10350 punct ", ",abolished
R6561 T10356 T10357 compound Snail,expression
R6562 T10357 T10350 nsubjpass expression,abolished
R6563 T10358 T10350 auxpass is,abolished
R6564 T10359 T10350 prep in,abolished
R6565 T10360 T10361 det the,buds
R6566 T10361 T10359 pobj buds,in
R6567 T10362 T10361 compound mutant,buds
R6568 T10363 T10361 compound hair,buds
R6569 T10364 T10350 punct ", ",abolished
R6570 T10365 T10350 cc and,abolished
R6571 T10366 T10367 advmod conversely,activated
R6572 T10367 T10350 conj activated,abolished
R6573 T10368 T10367 punct ", ",activated
R6574 T10369 T10367 prep in,activated
R6575 T10370 T10371 compound K14,Smad2
R6576 T10371 T10373 compound Smad2,skin
R6577 T10372 T10371 punct -,Smad2
R6578 T10373 T10369 pobj skin,in
R6579 T10374 T10367 punct ", ",activated
R6580 T10375 T10367 nsubjpass Snail,activated
R6581 T10376 T10367 auxpass is,activated
R6582 T10377 T10367 advmod ectopically,activated
R6583 T10378 T10348 punct .,demonstrate
R6584 T10380 T10381 advmod Moreover,indicate
R6585 T10382 T10381 punct ", ",indicate
R6586 T10383 T10384 poss our,studies
R6587 T10384 T10381 nsubj studies,indicate
R6588 T10385 T10386 advmod in,vitro
R6589 T10386 T10384 amod vitro,studies
R6590 T10387 T10388 mark that,cause
R6591 T10388 T10381 ccomp cause,indicate
R6592 T10389 T10390 advmod even,exposure
R6593 T10390 T10388 nsubj exposure,cause
R6594 T10391 T10390 amod sustained,exposure
R6595 T10392 T10393 compound TGF,β2
R6596 T10393 T10390 compound β2,exposure
R6597 T10394 T10393 punct -,β2
R6598 T10395 T10388 aux may,cause
R6599 T10396 T10397 advmod only,induction
R6600 T10397 T10388 dobj induction,cause
R6601 T10398 T10397 det a,induction
R6602 T10399 T10397 amod transient,induction
R6603 T10400 T10397 prep of,induction
R6604 T10401 T10400 pobj Snail,of
R6605 T10402 T10381 punct ", ",indicate
R6606 T10403 T10381 advcl offering,indicate
R6607 T10404 T10405 det a,explanation
R6608 T10405 T10403 dobj explanation,offering
R6609 T10406 T10405 amod possible,explanation
R6610 T10407 T10405 prep as,explanation
R6611 T10408 T10407 prep to,as
R6612 T10409 T10410 advmod why,expressed
R6613 T10410 T10408 pcomp expressed,to
R6614 T10411 T10410 nsubjpass Snail,expressed
R6615 T10412 T10410 auxpass is,expressed
R6616 T10413 T10414 advmod so,briefly
R6617 T10414 T10410 advmod briefly,expressed
R6618 T10415 T10410 prep during,expressed
R6619 T10416 T10417 compound hair,follicle
R6620 T10417 T10418 compound follicle,morphogenesis
R6621 T10418 T10415 pobj morphogenesis,during
R6622 T10419 T10381 punct .,indicate
R6623 T10421 T10422 det An,point
R6624 T10422 T10424 nsubj point,is
R6625 T10423 T10422 amod additional,point
R6626 T10425 T10422 amod worth,point
R6627 T10426 T10425 advcl mentioning,worth
R6628 T10427 T10428 mark that,resulted
R6629 T10428 T10424 ccomp resulted,is
R6630 T10429 T10430 amod prolonged,expression
R6631 T10430 T10428 nsubj expression,resulted
R6632 T10431 T10430 prep of,expression
R6633 T10432 T10433 compound Tg,Snail
R6634 T10433 T10431 pobj Snail,of
R6635 T10434 T10430 prep in,expression
R6636 T10435 T10436 amod postnatal,skin
R6637 T10436 T10434 pobj skin,in
R6638 T10437 T10428 prep in,resulted
R6639 T10438 T10439 amod morphological,signs
R6640 T10439 T10437 pobj signs,in
R6641 T10440 T10438 cc and,morphological
R6642 T10441 T10438 conj biochemical,morphological
R6643 T10442 T10439 prep of,signs
R6644 T10443 T10444 amod epithelial,transitions
R6645 T10444 T10442 pobj transitions,of
R6646 T10445 T10443 prep to,epithelial
R6647 T10446 T10445 amod mesenchymal,to
R6648 T10447 T10448 punct (,data
R6649 T10448 T10428 meta data,resulted
R6650 T10449 T10448 amod unpublished,data
R6651 T10450 T10448 punct ),data
R6652 T10451 T10428 punct ", ",resulted
R6653 T10452 T10428 advcl underscoring,resulted
R6654 T10453 T10454 advmod why,be
R6655 T10454 T10452 ccomp be,underscoring
R6656 T10455 T10456 amod transient,expression
R6657 T10456 T10454 nsubj expression,be
R6658 T10457 T10456 compound Snail,expression
R6659 T10458 T10454 aux may,be
R6660 T10459 T10460 advmod so,important
R6661 T10460 T10454 acomp important,be
R6662 T10461 T10454 prep during,be
R6663 T10462 T10463 amod normal,morphogenesis
R6664 T10463 T10461 pobj morphogenesis,during
R6665 T10464 T10465 compound hair,follicle
R6666 T10465 T10463 compound follicle,morphogenesis
R6667 T10466 T10467 punct [,18
R6668 T10467 T10424 parataxis 18,is
R6669 T10468 T10467 punct ],18
R6670 T10469 T10424 punct .,is
R6671 T10471 T10472 prep At,seemed
R6672 T10473 T10474 amod first,glance
R6673 T10474 T10471 pobj glance,At
R6674 T10475 T10472 punct ", ",seemed
R6675 T10476 T10477 det the,sparsity
R6676 T10477 T10472 nsubj sparsity,seemed
R6677 T10478 T10477 prep in,sparsity
R6678 T10479 T10480 compound hair,coat
R6679 T10480 T10478 pobj coat,in
R6680 T10481 T10480 prep of,coat
R6681 T10482 T10483 compound K14,Snail
R6682 T10483 T10485 compound Snail,mice
R6683 T10484 T10483 punct -,Snail
R6684 T10485 T10481 pobj mice,of
R6685 T10486 T10485 compound Tg,mice
R6686 T10487 T10472 oprd indicative,seemed
R6687 T10488 T10487 prep of,indicative
R6688 T10489 T10490 det a,defect
R6689 T10490 T10488 pobj defect,of
R6690 T10491 T10490 prep in,defect
R6691 T10492 T10493 compound follicle,formation
R6692 T10493 T10491 pobj formation,in
R6693 T10494 T10495 punct (,see
R6694 T10495 T10472 parataxis see,seemed
R6695 T10496 T10497 compound Figure,2A
R6696 T10497 T10495 dobj 2A,see
R6697 T10498 T10495 punct ),see
R6698 T10499 T10472 punct .,seemed
R6699 T10501 T10502 amod Closer,inspection
R6700 T10502 T10503 nsubj inspection,revealed
R6701 T10504 T10503 punct ", ",revealed
R6702 T10505 T10503 advmod however,revealed
R6703 T10506 T10503 punct ", ",revealed
R6704 T10507 T10508 mark that,penetrated
R6705 T10508 T10503 ccomp penetrated,revealed
R6706 T10509 T10510 neg not,hairs
R6707 T10510 T10508 nsubj hairs,penetrated
R6708 T10511 T10510 det all,hairs
R6709 T10512 T10513 det the,epidermis
R6710 T10513 T10508 dobj epidermis,penetrated
R6711 T10514 T10513 amod hyperthickened,epidermis
R6712 T10515 T10513 compound Tg,epidermis
R6713 T10516 T10503 punct .,revealed
R6714 T10518 T10519 amod Several,factors
R6715 T10519 T10520 nsubj factors,contribute
R6716 T10521 T10520 aux may,contribute
R6717 T10522 T10520 prep to,contribute
R6718 T10523 T10524 det the,development
R6719 T10524 T10522 pobj development,to
R6720 T10525 T10526 advmod seemingly,normal
R6721 T10526 T10524 amod normal,development
R6722 T10527 T10524 compound follicle,development
R6723 T10528 T10524 prep in,development
R6724 T10529 T10530 det these,mice
R6725 T10530 T10528 pobj mice,in
R6726 T10531 T10520 punct .,contribute
R6727 T10533 T10534 nummod One,factor
R6728 T10534 T10536 nsubj factor,is
R6729 T10535 T10534 amod obvious,factor
R6730 T10537 T10538 det the,promoter
R6731 T10538 T10536 attr promoter,is
R6732 T10539 T10538 compound K14,promoter
R6733 T10540 T10538 punct ", ",promoter
R6734 T10541 T10542 dep which,is
R6735 T10542 T10538 relcl is,promoter
R6736 T10543 T10542 acomp elevated,is
R6737 T10544 T10542 prep in,is
R6738 T10545 T10546 det the,layer
R6739 T10546 T10544 pobj layer,in
R6740 T10547 T10546 amod basal,layer
R6741 T10548 T10546 prep of,layer
R6742 T10549 T10550 det the,epidermis
R6743 T10550 T10548 pobj epidermis,of
R6744 T10551 T10546 cc and,layer
R6745 T10552 T10553 det the,sheath
R6746 T10553 T10546 conj sheath,layer
R6747 T10554 T10553 amod outer,sheath
R6748 T10555 T10553 compound root,sheath
R6749 T10556 T10553 punct (,sheath
R6750 T10557 T10553 appos ORS,sheath
R6751 T10558 T10553 punct ),sheath
R6752 T10559 T10553 prep of,sheath
R6753 T10560 T10561 det the,follicle
R6754 T10561 T10559 pobj follicle,of
R6755 T10562 T10561 compound hair,follicle
R6756 T10563 T10536 punct ", ",is
R6757 T10564 T10536 cc but,is
R6758 T10565 T10566 auxpass is,regulated
R6759 T10566 T10536 conj regulated,is
R6760 T10567 T10566 advmod markedly,regulated
R6761 T10568 T10566 advmod down,regulated
R6762 T10569 T10566 punct -,regulated
R6763 T10570 T10566 prep in,regulated
R6764 T10571 T10572 amod developing,buds
R6765 T10572 T10570 pobj buds,in
R6766 T10573 T10572 amod embryonic,buds
R6767 T10574 T10572 compound hair,buds
R6768 T10575 T10576 advmod as,as
R6769 T10576 T10570 cc as,in
R6770 T10577 T10576 advmod well,as
R6771 T10578 T10570 conj in,in
R6772 T10579 T10580 det the,cells
R6773 T10580 T10578 pobj cells,in
R6774 T10581 T10580 amod postnatal,cells
R6775 T10582 T10580 compound hair,cells
R6776 T10583 T10580 compound progenitor,cells
R6777 T10584 T10536 punct .,is
R6778 T10586 T10587 det The,promoter
R6779 T10587 T10589 nsubj promoter,is
R6780 T10588 T10587 compound K14,promoter
R6781 T10590 T10589 advmod also,is
R6782 T10591 T10592 advmod less,active
R6783 T10592 T10589 acomp active,is
R6784 T10593 T10592 prep in,active
R6785 T10594 T10595 det the,ORS
R6786 T10595 T10593 pobj ORS,in
R6787 T10596 T10592 prep than,active
R6788 T10597 T10596 pcomp epidermis,than
R6789 T10598 T10589 cc and,is
R6790 T10599 T10600 advmod hence,account
R6791 T10600 T10589 conj account,is
R6792 T10601 T10600 nsubj this,account
R6793 T10602 T10600 aux might,account
R6794 T10603 T10600 advmod also,account
R6795 T10604 T10600 prep for,account
R6796 T10605 T10606 det the,lack
R6797 T10606 T10604 pobj lack,for
R6798 T10607 T10606 prep of,lack
R6799 T10608 T10609 amod apparent,response
R6800 T10609 T10607 pobj response,of
R6801 T10610 T10609 prep of,response
R6802 T10611 T10612 det the,ORS
R6803 T10612 T10610 pobj ORS,of
R6804 T10613 T10609 prep to,response
R6805 T10614 T10615 amod ectopic,Snail
R6806 T10615 T10613 pobj Snail,to
R6807 T10616 T10600 punct .,account
R6808 T10618 T10619 amod Additional,factors
R6809 T10619 T10621 nsubj factors,be
R6810 T10620 T10619 amod contributing,factors
R6811 T10622 T10621 aux could,be
R6812 T10623 T10624 det the,multiplicity
R6813 T10624 T10621 attr multiplicity,be
R6814 T10625 T10624 prep of,multiplicity
R6815 T10626 T10627 compound Snail,members
R6816 T10627 T10625 pobj members,of
R6817 T10628 T10627 compound family,members
R6818 T10629 T10627 cc and,members
R6819 T10630 T10631 poss their,expression
R6820 T10631 T10627 conj expression,members
R6821 T10632 T10631 amod differential,expression
R6822 T10633 T10627 punct ", ",members
R6823 T10634 T10635 det the,saturation
R6824 T10635 T10627 appos saturation,members
R6825 T10636 T10635 punct ", ",saturation
R6826 T10637 T10635 cc and,saturation
R6827 T10638 T10637 punct /,and
R6828 T10639 T10637 cc or,and
R6829 T10640 T10635 conj diversity,saturation
R6830 T10641 T10635 prep of,saturation
R6831 T10642 T10643 amod regulatory,mechanisms
R6832 T10643 T10641 pobj mechanisms,of
R6833 T10644 T10645 dep that,govern
R6834 T10645 T10643 relcl govern,mechanisms
R6835 T10646 T10647 compound AJ,formation
R6836 T10647 T10645 dobj formation,govern
R6837 T10648 T10647 punct ", ",formation
R6838 T10649 T10647 conj migration,formation
R6839 T10650 T10649 punct ", ",migration
R6840 T10651 T10649 cc and,migration
R6841 T10652 T10649 conj proliferation,migration
R6842 T10653 T10645 prep in,govern
R6843 T10654 T10655 det the,ORS
R6844 T10655 T10653 pobj ORS,in
R6845 T10656 T10655 compound follicle,ORS
R6846 T10657 T10621 punct .,be
R6847 T10659 T10660 csubj Distinguishing,await
R6848 T10661 T10659 prep between,Distinguishing
R6849 T10662 T10663 det these,possibilities
R6850 T10663 T10661 pobj possibilities,between
R6851 T10664 T10660 aux must,await
R6852 T10665 T10666 det the,generation
R6853 T10666 T10660 dobj generation,await
R6854 T10667 T10666 prep of,generation
R6855 T10668 T10667 pobj mice,of
R6856 T10669 T10668 acl harboring,mice
R6857 T10670 T10671 npadvmod skin,specific
R6858 T10671 T10673 amod specific,mutations
R6859 T10672 T10671 punct -,specific
R6860 T10673 T10669 dobj mutations,harboring
R6861 T10674 T10673 nmod loss,mutations
R6862 T10675 T10674 punct -,loss
R6863 T10676 T10674 prep of,loss
R6864 T10677 T10676 punct -,of
R6865 T10678 T10676 pobj function,of
R6866 T10679 T10673 compound Snail,mutations
R6867 T10680 T10660 punct .,await
R6876 T11058 T11057 prep between,Links
R6877 T11059 T11058 pobj Signaling,between
R6878 T11060 T11059 punct ", ",Signaling
R6879 T11061 T11062 amod Transcriptional,Cascades
R6880 T11062 T11059 conj Cascades,Signaling
R6881 T11063 T11062 punct ", ",Cascades
R6882 T11064 T11065 amod Epithelial,Remodeling
R6883 T11065 T11062 conj Remodeling,Cascades
R6884 T11066 T11065 punct ", ",Remodeling
R6885 T11067 T11065 cc and,Remodeling
R6886 T11068 T11065 conj Proliferation,Remodeling
R6887 T11069 T11057 prep in,Links
R6888 T11070 T11071 det the,Bud
R6889 T11071 T11069 pobj Bud,in
R6890 T11072 T11071 compound Hair,Bud
R6891 T11074 T11075 advmod Previously,discovered
R6892 T11076 T11075 punct ", ",discovered
R6893 T11077 T11075 nsubj we,discovered
R6894 T11078 T11079 mark that,regulated
R6895 T11079 T11075 ccomp regulated,discovered
R6896 T11080 T11079 advmod early,regulated
R6897 T11081 T11080 prep during,early
R6898 T11082 T11083 compound hair,follicle
R6899 T11083 T11084 compound follicle,morphogenesis
R6900 T11084 T11081 pobj morphogenesis,during
R6901 T11085 T11079 punct ", ",regulated
R6902 T11086 T11087 compound E,cadherin
R6903 T11087 T11089 compound cadherin,expression
R6904 T11088 T11087 punct -,cadherin
R6905 T11089 T11079 nsubjpass expression,regulated
R6906 T11090 T11089 compound gene,expression
R6907 T11091 T11079 auxpass is,regulated
R6908 T11092 T11079 advmod down,regulated
R6909 T11093 T11079 punct -,regulated
R6910 T11094 T11079 advmod concomitantly,regulated
R6911 T11095 T11079 prep with,regulated
R6912 T11096 T11097 det the,invagination
R6913 T11097 T11095 pobj invagination,with
R6914 T11098 T11097 prep of,invagination
R6915 T11099 T11100 amod developing,cells
R6916 T11100 T11098 pobj cells,of
R6917 T11101 T11100 compound bud,cells
R6918 T11102 T11097 prep into,invagination
R6919 T11103 T11104 det the,skin
R6920 T11104 T11102 pobj skin,into
R6921 T11105 T11106 punct [,4
R6922 T11106 T11075 parataxis 4,discovered
R6923 T11107 T11106 punct ],4
R6924 T11108 T11075 punct .,discovered
R6925 T11110 T11111 mark Because,correlated
R6926 T11111 T11117 advcl correlated,intrigued
R6927 T11112 T11113 det the,timing
R6928 T11113 T11111 nsubj timing,correlated
R6929 T11114 T11113 prep of,timing
R6930 T11115 T11116 det this,event
R6931 T11116 T11114 pobj event,of
R6932 T11118 T11111 prep with,correlated
R6933 T11119 T11120 det the,activation
R6934 T11120 T11118 pobj activation,with
R6935 T11121 T11120 prep of,activation
R6936 T11122 T11123 det a,complex
R6937 T11123 T11121 pobj complex,of
R6938 T11124 T11125 nmod LEF,factor
R6939 T11125 T11123 compound factor,complex
R6940 T11126 T11124 punct -,LEF
R6941 T11127 T11124 nummod 1,LEF
R6942 T11128 T11124 punct /,LEF
R6943 T11129 T11130 compound β,catenin
R6944 T11130 T11124 appos catenin,LEF
R6945 T11131 T11130 punct -,catenin
R6946 T11132 T11125 compound transcription,factor
R6947 T11133 T11134 punct [,20
R6948 T11134 T11111 parataxis 20,correlated
R6949 T11135 T11134 punct ],20
R6950 T11136 T11117 punct ", ",intrigued
R6951 T11137 T11117 nsubjpass we,intrigued
R6952 T11138 T11117 auxpass were,intrigued
R6953 T11139 T11117 agent by,intrigued
R6954 T11140 T11141 det the,presence
R6955 T11141 T11139 pobj presence,by
R6956 T11142 T11141 prep of,presence
R6957 T11143 T11144 det a,site
R6958 T11144 T11142 pobj site,of
R6959 T11145 T11144 amod putative,site
R6960 T11146 T11144 nmod LEF,site
R6961 T11147 T11146 punct -,LEF
R6962 T11148 T11146 nummod 1,LEF
R6963 T11149 T11146 punct /,LEF
R6964 T11150 T11146 appos TCF,LEF
R6965 T11151 T11144 compound binding,site
R6966 T11152 T11141 prep in,presence
R6967 T11153 T11154 det the,promoter
R6968 T11154 T11152 pobj promoter,in
R6969 T11155 T11156 compound E,cadherin
R6970 T11156 T11154 compound cadherin,promoter
R6971 T11157 T11156 punct -,cadherin
R6972 T11158 T11117 punct .,intrigued
R6973 T11160 T11161 nsubj This,prompted
R6974 T11162 T11163 det an,investigation
R6975 T11163 T11161 dobj investigation,prompted
R6976 T11164 T11165 dep that,led
R6977 T11165 T11163 relcl led,investigation
R6978 T11166 T11165 advmod subsequently,led
R6979 T11167 T11165 prep to,led
R6980 T11168 T11169 poss our,discovery
R6981 T11169 T11167 pobj discovery,to
R6982 T11170 T11171 mark that,contribute
R6983 T11171 T11169 acl contribute,discovery
R6984 T11172 T11171 nsubj LEF,contribute
R6985 T11173 T11172 punct -,LEF
R6986 T11174 T11172 nummod 1,LEF
R6987 T11175 T11172 punct /,LEF
R6988 T11176 T11177 compound β,catenin
R6989 T11177 T11172 appos catenin,LEF
R6990 T11178 T11177 punct -,catenin
R6991 T11179 T11171 aux can,contribute
R6992 T11180 T11171 prep to,contribute
R6993 T11181 T11180 pobj repression,to
R6994 T11182 T11181 prep of,repression
R6995 T11183 T11184 compound E,cadherin
R6996 T11184 T11186 compound cadherin,expression
R6997 T11185 T11184 punct -,cadherin
R6998 T11186 T11182 pobj expression,of
R6999 T11187 T11186 compound gene,expression
R7000 T11188 T11171 prep in,contribute
R7001 T11189 T11190 compound skin,keratinocytes
R7002 T11190 T11188 pobj keratinocytes,in
R7003 T11191 T11192 punct [,4
R7004 T11192 T11161 parataxis 4,prompted
R7005 T11193 T11192 punct ],4
R7006 T11194 T11161 punct .,prompted
R7007 T11196 T11197 prep In,noted
R7008 T11198 T11199 det the,course
R7009 T11199 T11196 pobj course,In
R7010 T11200 T11199 prep of,course
R7011 T11201 T11202 det these,studies
R7012 T11202 T11200 pobj studies,of
R7013 T11203 T11197 punct ", ",noted
R7014 T11204 T11197 nsubj we,noted
R7015 T11205 T11197 advmod also,noted
R7016 T11206 T11207 mark that,contribute
R7017 T11207 T11197 ccomp contribute,noted
R7018 T11208 T11207 nsubj Snail,contribute
R7019 T11209 T11207 aux can,contribute
R7020 T11210 T11207 advmod also,contribute
R7021 T11211 T11207 prep to,contribute
R7022 T11212 T11213 det this,process
R7023 T11213 T11211 pobj process,to
R7024 T11214 T11207 prep in,contribute
R7025 T11215 T11214 pobj keratinocytes,in
R7026 T11216 T11217 advmod in,vitro
R7027 T11217 T11207 advmod vitro,contribute
R7028 T11218 T11197 punct ", ",noted
R7029 T11219 T11197 cc and,noted
R7030 T11220 T11221 poss our,studies
R7031 T11221 T11223 nsubj studies,revealed
R7032 T11222 T11221 amod present,studies
R7033 T11223 T11197 conj revealed,noted
R7034 T11224 T11225 mark that,expressed
R7035 T11225 T11223 ccomp expressed,revealed
R7036 T11226 T11225 nsubjpass Snail,expressed
R7037 T11227 T11225 auxpass is,expressed
R7038 T11228 T11225 prep at,expressed
R7039 T11229 T11230 det the,place
R7040 T11230 T11228 pobj place,at
R7041 T11231 T11230 amod right,place
R7042 T11232 T11230 cc and,place
R7043 T11233 T11230 conj time,place
R7044 T11234 T11235 aux to,be
R7045 T11235 T11230 advcl be,place
R7046 T11236 T11237 advmod physiologically,relevant
R7047 T11237 T11235 acomp relevant,be
R7048 T11238 T11235 prep in,be
R7049 T11239 T11240 det the,process
R7050 T11240 T11238 pobj process,in
R7051 T11241 T11223 punct .,revealed
R7052 T11243 T11244 prep In,abrogated
R7053 T11245 T11246 npadvmod noggin,null
R7054 T11246 T11248 amod null,skin
R7055 T11247 T11246 punct -,null
R7056 T11248 T11243 pobj skin,In
R7057 T11249 T11248 amod embryonic,skin
R7058 T11250 T11244 punct ", ",abrogated
R7059 T11251 T11252 nmod LEF,expression
R7060 T11252 T11244 nsubjpass expression,abrogated
R7061 T11253 T11251 punct -,LEF
R7062 T11254 T11251 nummod 1,LEF
R7063 T11255 T11252 cc and,expression
R7064 T11256 T11257 amod subsequent,activation
R7065 T11257 T11252 conj activation,expression
R7066 T11258 T11257 prep of,activation
R7067 T11259 T11260 det the,gene
R7068 T11260 T11258 pobj gene,of
R7069 T11261 T11260 nmod LEF,gene
R7070 T11262 T11261 punct -,LEF
R7071 T11263 T11261 nummod 1,LEF
R7072 T11264 T11261 punct /,LEF
R7073 T11265 T11266 compound β,catenin
R7074 T11266 T11261 appos catenin,LEF
R7075 T11267 T11266 punct -,catenin
R7076 T11268 T11260 compound reporter,gene
R7077 T11269 T11244 auxpass is,abrogated
R7078 T11270 T11244 prep in,abrogated
R7079 T11271 T11272 det the,placodes
R7080 T11272 T11270 pobj placodes,in
R7081 T11273 T11272 amod developing,placodes
R7082 T11274 T11244 punct .,abrogated
R7083 T11276 T11277 det The,failure
R7084 T11277 T11279 nsubj failure,underscores
R7085 T11278 T11277 amod corresponding,failure
R7086 T11280 T11277 prep of,failure
R7087 T11281 T11282 nmod E,cadherin
R7088 T11282 T11284 nmod cadherin,regulation
R7089 T11283 T11282 punct -,cadherin
R7090 T11284 T11280 pobj regulation,of
R7091 T11285 T11284 amod down,regulation
R7092 T11286 T11284 punct -,regulation
R7093 T11287 T11288 det the,importance
R7094 T11288 T11279 dobj importance,underscores
R7095 T11289 T11288 prep of,importance
R7096 T11290 T11291 compound Wnt,noggin
R7097 T11291 T11293 compound noggin,signaling
R7098 T11292 T11291 punct /,noggin
R7099 T11293 T11289 pobj signaling,of
R7100 T11294 T11288 prep in,importance
R7101 T11295 T11294 pcomp regulating,in
R7102 T11296 T11297 det this,event
R7103 T11297 T11295 dobj event,regulating
R7104 T11298 T11295 prep in,regulating
R7105 T11299 T11300 compound follicle,morphogenesis
R7106 T11300 T11298 pobj morphogenesis,in
R7107 T11301 T11302 punct [,4
R7108 T11302 T11279 parataxis 4,underscores
R7109 T11303 T11302 punct ],4
R7110 T11304 T11279 punct .,underscores
R7111 T11306 T11307 amod Conditional,studies
R7112 T11307 T11310 nsubj studies,be
R7113 T11308 T11309 compound gene,targeting
R7114 T11309 T11307 compound targeting,studies
R7115 T11311 T11310 aux will,be
R7116 T11312 T11310 acomp necessary,be
R7117 T11313 T11314 aux to,establish
R7118 T11314 T11310 advcl establish,be
R7119 T11315 T11316 mark whether,contribute
R7120 T11316 T11314 ccomp contribute,establish
R7121 T11317 T11318 compound Snail,members
R7122 T11318 T11316 nsubj members,contribute
R7123 T11319 T11318 compound family,members
R7124 T11320 T11316 advmod also,contribute
R7125 T11321 T11316 prep to,contribute
R7126 T11322 T11323 det the,regulation
R7127 T11323 T11321 pobj regulation,to
R7128 T11324 T11323 amod down,regulation
R7129 T11325 T11323 punct -,regulation
R7130 T11326 T11323 prep in,regulation
R7131 T11327 T11328 compound E,cadherin
R7132 T11328 T11330 compound cadherin,expression
R7133 T11329 T11328 punct -,cadherin
R7134 T11330 T11326 pobj expression,in
R7135 T11331 T11330 compound gene,expression
R7136 T11332 T11333 dep that,occurs
R7137 T11333 T11323 relcl occurs,regulation
R7138 T11334 T11333 prep during,occurs
R7139 T11335 T11336 compound follicle,formation
R7140 T11336 T11334 pobj formation,during
R7141 T11337 T11310 punct .,be
R7142 T11339 T11340 advmod However,is
R7143 T11341 T11340 punct ", ",is
R7144 T11342 T11340 nsubj it,is
R7145 T11343 T11340 acomp intriguing,is
R7146 T11344 T11345 mark that,displayed
R7147 T11345 T11340 ccomp displayed,is
R7148 T11346 T11347 compound K14,Snail
R7149 T11347 T11349 compound Snail,epidermis
R7150 T11348 T11347 punct -,Snail
R7151 T11349 T11345 nsubj epidermis,displayed
R7152 T11350 T11349 compound Tg,epidermis
R7153 T11351 T11352 det a,regulation
R7154 T11352 T11345 dobj regulation,displayed
R7155 T11353 T11352 amod marked,regulation
R7156 T11354 T11352 amod down,regulation
R7157 T11355 T11352 punct -,regulation
R7158 T11356 T11352 prep in,regulation
R7159 T11357 T11358 compound E,cadherin
R7160 T11358 T11360 compound cadherin,expression
R7161 T11359 T11358 punct -,cadherin
R7162 T11360 T11356 pobj expression,in
R7163 T11361 T11345 punct ", ",displayed
R7164 T11362 T11363 advmod thereby,demonstrating
R7165 T11363 T11345 advcl demonstrating,displayed
R7166 T11364 T11365 poss its,potential
R7167 T11365 T11363 dobj potential,demonstrating
R7168 T11366 T11367 aux to,do
R7169 T11367 T11365 acl do,potential
R7170 T11368 T11367 advmod so,do
R7171 T11369 T11367 prep in,do
R7172 T11370 T11369 pobj skin,in
R7173 T11371 T11340 punct .,is
R7174 T11373 T11374 poss Our,findings
R7175 T11374 T11376 nsubj findings,showed
R7176 T11375 T11374 amod prior,findings
R7177 T11377 T11378 mark that,impaired
R7178 T11378 T11376 ccomp impaired,showed
R7179 T11379 T11378 prep by,impaired
R7180 T11380 T11379 pcomp elevating,by
R7181 T11381 T11382 compound E,cadherin
R7182 T11382 T11384 compound cadherin,levels
R7183 T11383 T11382 punct -,cadherin
R7184 T11384 T11380 dobj levels,elevating
R7185 T11385 T11379 cc or,by
R7186 T11386 T11379 conj by,by
R7187 T11387 T11388 advmod conditionally,ablating
R7188 T11388 T11386 pcomp ablating,by
R7189 T11389 T11390 compound α,catenin
R7190 T11390 T11388 dobj catenin,ablating
R7191 T11391 T11390 punct -,catenin
R7192 T11392 T11378 punct ", ",impaired
R7193 T11393 T11394 compound hair,follicle
R7194 T11394 T11395 compound follicle,morphogenesis
R7195 T11395 T11378 nsubjpass morphogenesis,impaired
R7196 T11396 T11378 aux can,impaired
R7197 T11397 T11378 auxpass be,impaired
R7198 T11398 T11399 punct [,7
R7199 T11399 T11376 parataxis 7,showed
R7200 T11400 T11399 nummod 4,7
R7201 T11401 T11399 punct ",",7
R7202 T11402 T11399 punct ],7
R7203 T11403 T11376 punct .,showed
R7204 T11405 T11406 det The,hyperproliferation
R7205 T11406 T11409 nsubj hyperproliferation,led
R7206 T11407 T11406 amod marked,hyperproliferation
R7207 T11408 T11406 amod epidermal,hyperproliferation
R7208 T11410 T11406 acl seen,hyperproliferation
R7209 T11411 T11410 prep in,seen
R7210 T11412 T11413 det the,skin
R7211 T11413 T11411 pobj skin,in
R7212 T11414 T11415 compound K14,Snail
R7213 T11415 T11413 compound Snail,skin
R7214 T11416 T11415 punct -,Snail
R7215 T11417 T11413 compound Tg,skin
R7216 T11418 T11406 punct ", ",hyperproliferation
R7217 T11419 T11406 acl coupled,hyperproliferation
R7218 T11420 T11419 prep with,coupled
R7219 T11421 T11422 det the,suppression
R7220 T11422 T11420 pobj suppression,with
R7221 T11423 T11422 amod converse,suppression
R7222 T11424 T11422 prep of,suppression
R7223 T11425 T11424 pobj proliferation,of
R7224 T11426 T11425 cc and,proliferation
R7225 T11427 T11425 conj Snail,proliferation
R7226 T11428 T11422 prep in,suppression
R7227 T11429 T11430 compound TGF,β2
R7228 T11430 T11432 npadvmod β2,null
R7229 T11431 T11430 punct -,β2
R7230 T11432 T11434 amod null,buds
R7231 T11433 T11432 punct -,null
R7232 T11434 T11428 pobj buds,in
R7233 T11435 T11434 compound hair,buds
R7234 T11436 T11409 dobj us,led
R7235 T11437 T11438 aux to,wonder
R7236 T11438 T11409 xcomp wonder,led
R7237 T11439 T11440 mark whether,have
R7238 T11440 T11438 ccomp have,wonder
R7239 T11441 T11442 det the,regulation
R7240 T11442 T11440 nsubj regulation,have
R7241 T11443 T11442 amod down,regulation
R7242 T11444 T11442 punct -,regulation
R7243 T11445 T11442 prep of,regulation
R7244 T11446 T11447 compound E,cadherin
R7245 T11447 T11445 pobj cadherin,of
R7246 T11448 T11447 punct -,cadherin
R7247 T11449 T11442 prep during,regulation
R7248 T11450 T11451 compound follicle,morphogenesis
R7249 T11451 T11449 pobj morphogenesis,during
R7250 T11452 T11440 aux might,have
R7251 T11453 T11454 det a,impact
R7252 T11454 T11440 dobj impact,have
R7253 T11455 T11454 amod direct,impact
R7254 T11456 T11454 prep on,impact
R7255 T11457 T11456 pcomp elevating,on
R7256 T11458 T11459 det the,state
R7257 T11459 T11457 dobj state,elevating
R7258 T11460 T11459 amod proliferative,state
R7259 T11461 T11459 prep of,state
R7260 T11462 T11463 det these,cells
R7261 T11463 T11461 pobj cells,of
R7262 T11464 T11409 punct .,led
R7263 T11466 T11467 poss Our,studies
R7264 T11467 T11469 nsubj studies,suggested
R7265 T11468 T11467 compound Tg,studies
R7266 T11470 T11471 mark that,is
R7267 T11471 T11469 ccomp is,suggested
R7268 T11472 T11471 punct ", ",is
R7269 T11473 T11474 advmod at,least
R7270 T11474 T11475 advmod least,in
R7271 T11475 T11476 prep in,through
R7272 T11476 T11471 prep through,is
R7273 T11477 T11475 pobj part,in
R7274 T11478 T11479 poss its,regulation
R7275 T11479 T11476 pobj regulation,through
R7276 T11480 T11479 prep of,regulation
R7277 T11481 T11482 compound E,cadherin
R7278 T11482 T11480 pobj cadherin,of
R7279 T11483 T11482 punct -,cadherin
R7280 T11484 T11471 punct ", ",is
R7281 T11485 T11471 nsubj Snail,is
R7282 T11486 T11471 acomp able,is
R7283 T11487 T11488 aux to,influence
R7284 T11488 T11486 xcomp influence,able
R7285 T11489 T11490 det the,localization
R7286 T11490 T11488 dobj localization,influence
R7287 T11491 T11490 amod subcellular,localization
R7288 T11492 T11490 prep of,localization
R7289 T11493 T11494 det a,variety
R7290 T11494 T11492 pobj variety,of
R7291 T11495 T11494 prep of,variety
R7292 T11496 T11497 npadvmod AJ,associated
R7293 T11497 T11499 amod associated,proteins
R7294 T11498 T11497 punct -,associated
R7295 T11499 T11495 pobj proteins,of
R7296 T11500 T11469 punct .,suggested
R7297 T11502 T11503 nsubj One,appears
R7298 T11504 T11502 prep of,One
R7299 T11505 T11504 pobj these,of
R7300 T11506 T11507 aux to,be
R7301 T11507 T11503 xcomp be,appears
R7302 T11508 T11507 attr Ajuba,be
R7303 T11509 T11508 punct ", ",Ajuba
R7304 T11510 T11511 dep which,shown
R7305 T11511 T11508 relcl shown,Ajuba
R7306 T11512 T11511 auxpass was,shown
R7307 T11513 T11511 advmod previously,shown
R7308 T11514 T11515 aux to,have
R7309 T11515 T11511 xcomp have,shown
R7310 T11516 T11517 det the,capacity
R7311 T11517 T11515 dobj capacity,have
R7312 T11518 T11517 amod dual,capacity
R7313 T11519 T11520 aux to,bind
R7314 T11520 T11517 acl bind,capacity
R7315 T11521 T11520 dobj Grb,bind
R7316 T11522 T11521 punct -,Grb
R7317 T11523 T11521 nummod 2,Grb
R7318 T11524 T11525 advmod as,as
R7319 T11525 T11521 cc as,Grb
R7320 T11526 T11525 advmod well,as
R7321 T11527 T11528 compound α,catenin
R7322 T11528 T11521 conj catenin,Grb
R7323 T11529 T11528 punct -,catenin
R7324 T11530 T11531 punct [,10
R7325 T11531 T11503 parataxis 10,appears
R7326 T11532 T11531 nummod 9,10
R7327 T11533 T11531 punct ",",10
R7328 T11534 T11531 punct ],10
R7329 T11535 T11503 punct .,appears
R7330 T11537 T11538 poss Our,studies
R7331 T11538 T11539 nsubj studies,revealed
R7332 T11540 T11541 mark that,develops
R7333 T11541 T11539 ccomp develops,revealed
R7334 T11542 T11541 prep in,develops
R7335 T11543 T11544 compound skin,keratinocytes
R7336 T11544 T11542 pobj keratinocytes,in
R7337 T11545 T11546 dep that,harbor
R7338 T11546 T11544 advcl harbor,keratinocytes
R7339 T11547 T11546 preconj either,harbor
R7340 T11548 T11549 det a,mutation
R7341 T11549 T11546 dobj mutation,harbor
R7342 T11550 T11549 amod conditional,mutation
R7343 T11551 T11549 amod null,mutation
R7344 T11552 T11546 prep in,harbor
R7345 T11553 T11554 compound α,catenin
R7346 T11554 T11552 pobj catenin,in
R7347 T11555 T11554 punct -,catenin
R7348 T11556 T11546 cc or,harbor
R7349 T11557 T11558 dep that,overexpress
R7350 T11558 T11546 conj overexpress,harbor
R7351 T11559 T11558 dobj Snail,overexpress
R7352 T11560 T11541 punct ", ",develops
R7353 T11561 T11541 nsubj Ajuba,develops
R7354 T11562 T11563 det an,interaction
R7355 T11563 T11541 dobj interaction,develops
R7356 T11564 T11563 prep with,interaction
R7357 T11565 T11564 pobj Grb,with
R7358 T11566 T11565 punct -,Grb
R7359 T11567 T11565 nummod 2,Grb
R7360 T11568 T11569 dep that,observed
R7361 T11569 T11563 relcl observed,interaction
R7362 T11570 T11569 auxpass is,observed
R7363 T11571 T11569 advmod otherwise,observed
R7364 T11572 T11569 neg not,observed
R7365 T11573 T11569 prep in,observed
R7366 T11574 T11575 compound WT,keratinocytes
R7367 T11575 T11573 pobj keratinocytes,in
R7368 T11576 T11539 punct .,revealed
R7369 T11578 T11579 det The,abilities
R7370 T11579 T11581 nsubj abilities,suggest
R7371 T11580 T11579 amod corresponding,abilities
R7372 T11582 T11579 prep of,abilities
R7373 T11583 T11584 preconj either,keratinocytes
R7374 T11584 T11582 pobj keratinocytes,of
R7375 T11585 T11586 npadvmod Snail,transfected
R7376 T11586 T11584 amod transfected,keratinocytes
R7377 T11587 T11586 punct -,transfected
R7378 T11588 T11586 cc or,transfected
R7379 T11589 T11590 npadvmod Ajuba,transfected
R7380 T11590 T11586 conj transfected,transfected
R7381 T11591 T11590 punct -,transfected
R7382 T11592 T11593 aux to,exhibit
R7383 T11593 T11579 acl exhibit,abilities
R7384 T11594 T11595 amod elevated,activation
R7385 T11595 T11593 dobj activation,exhibit
R7386 T11596 T11595 prep of,activation
R7387 T11597 T11598 det the,pathway
R7388 T11598 T11596 pobj pathway,of
R7389 T11599 T11600 compound Ras,MAPK
R7390 T11600 T11598 compound MAPK,pathway
R7391 T11601 T11600 punct -,MAPK
R7392 T11602 T11603 mark that,be
R7393 T11603 T11581 ccomp be,suggest
R7394 T11604 T11605 det the,association
R7395 T11605 T11603 nsubj association,be
R7396 T11606 T11605 nmod Grb,association
R7397 T11607 T11606 punct -,Grb
R7398 T11608 T11606 nummod 2,Grb
R7399 T11609 T11605 prep of,association
R7400 T11610 T11609 pobj Ajuba,of
R7401 T11611 T11603 prep under,be
R7402 T11612 T11611 pobj conditions,under
R7403 T11613 T11612 prep of,conditions
R7404 T11614 T11615 amod reduced,levels
R7405 T11615 T11613 pobj levels,of
R7406 T11616 T11615 prep of,levels
R7407 T11617 T11618 compound AJ,proteins
R7408 T11618 T11616 pobj proteins,of
R7409 T11619 T11603 aux may,be
R7410 T11620 T11621 advmod directly,relevant
R7411 T11621 T11603 acomp relevant,be
R7412 T11622 T11621 prep to,relevant
R7413 T11623 T11624 det the,parallel
R7414 T11624 T11622 pobj parallel,to
R7415 T11625 T11624 prep in,parallel
R7416 T11626 T11625 pobj hyperproliferation,in
R7417 T11627 T11581 punct .,suggest
R7418 T11629 T11630 prep In,shown
R7419 T11631 T11632 amod stable,lines
R7420 T11632 T11629 pobj lines,In
R7421 T11633 T11632 amod epithelial,lines
R7422 T11634 T11635 punct (,kidney
R7423 T11635 T11632 parataxis kidney,lines
R7424 T11636 T11635 advmod i.e.,kidney
R7425 T11637 T11635 punct ", ",kidney
R7426 T11638 T11639 nmod Madin,Darby
R7427 T11639 T11635 nmod Darby,kidney
R7428 T11640 T11639 punct -,Darby
R7429 T11641 T11635 amod canine,kidney
R7430 T11642 T11635 punct ", ",kidney
R7431 T11643 T11635 cc or,kidney
R7432 T11644 T11635 conj MDCK,kidney
R7433 T11645 T11635 punct ),kidney
R7434 T11646 T11632 compound cell,lines
R7435 T11647 T11630 punct ", ",shown
R7436 T11648 T11630 nsubjpass Snail,shown
R7437 T11649 T11630 aux has,shown
R7438 T11650 T11630 auxpass been,shown
R7439 T11651 T11652 aux to,block
R7440 T11652 T11630 xcomp block,shown
R7441 T11653 T11654 compound cell,cycle
R7442 T11654 T11655 compound cycle,progression
R7443 T11655 T11652 dobj progression,block
R7444 T11656 T11652 cc and,block
R7445 T11657 T11652 conj promote,block
R7446 T11658 T11659 nmod motility,changes
R7447 T11659 T11657 dobj changes,promote
R7448 T11660 T11658 cc and,motility
R7449 T11661 T11658 conj shape,motility
R7450 T11662 T11657 prep for,promote
R7451 T11663 T11662 pobj invasion,for
R7452 T11664 T11665 punct [,47
R7453 T11665 T11630 parataxis 47,shown
R7454 T11666 T11665 punct ],47
R7455 T11667 T11630 punct .,shown
R7456 T11669 T11670 mark While,are
R7457 T11670 T11675 advcl are,differ
R7458 T11671 T11672 poss our,studies
R7459 T11672 T11670 nsubj studies,are
R7460 T11673 T11674 advmod in,vivo
R7461 T11674 T11672 amod vivo,studies
R7462 T11676 T11670 acomp consistent,are
R7463 T11677 T11676 prep with,consistent
R7464 T11678 T11679 det a,role
R7465 T11679 T11677 pobj role,with
R7466 T11680 T11679 prep for,role
R7467 T11681 T11680 pobj Snail,for
R7468 T11682 T11679 prep in,role
R7469 T11683 T11684 nmod motility,remodeling
R7470 T11684 T11682 pobj remodeling,in
R7471 T11685 T11683 cc and,motility
R7472 T11686 T11683 conj epithelial,motility
R7473 T11687 T11675 punct ", ",differ
R7474 T11688 T11675 nsubj they,differ
R7475 T11689 T11675 prep with,differ
R7476 T11690 T11689 pobj respect,with
R7477 T11691 T11690 prep to,respect
R7478 T11692 T11693 poss Snail,effects
R7479 T11693 T11691 pobj effects,to
R7480 T11694 T11692 case 's,Snail
R7481 T11695 T11693 amod apparent,effects
R7482 T11696 T11693 amod proliferative,effects
R7483 T11697 T11675 punct .,differ
R7484 T11699 T11700 advmod A,priori
R7485 T11700 T11701 advmod priori,be
R7486 T11702 T11701 punct ", ",be
R7487 T11703 T11701 nsubj this,be
R7488 T11704 T11701 aux could,be
R7489 T11705 T11701 advmod simply,be
R7490 T11706 T11701 prep due,be
R7491 T11707 T11706 pcomp to,due
R7492 T11708 T11706 pobj variations,due
R7493 T11709 T11708 prep in,variations
R7494 T11710 T11711 det the,response
R7495 T11711 T11709 pobj response,in
R7496 T11712 T11711 prep of,response
R7497 T11713 T11714 amod different,types
R7498 T11714 T11712 pobj types,of
R7499 T11715 T11714 compound cell,types
R7500 T11716 T11711 prep to,response
R7501 T11717 T11718 compound Snail,expression
R7502 T11718 T11716 pobj expression,to
R7503 T11719 T11701 punct .,be
R7504 T11721 T11722 advmod Alternatively,be
R7505 T11723 T11722 punct ", ",be
R7506 T11724 T11725 det these,differences
R7507 T11725 T11722 nsubj differences,be
R7508 T11726 T11722 aux may,be
R7509 T11727 T11722 acomp relevant,be
R7510 T11728 T11727 prep to,relevant
R7511 T11729 T11730 det the,benefit
R7512 T11730 T11728 pobj benefit,to
R7513 T11731 T11730 prep of,benefit
R7514 T11732 T11731 pcomp using,of
R7515 T11733 T11734 compound mouse,models
R7516 T11734 T11732 dobj models,using
R7517 T11735 T11736 aux to,reveal
R7518 T11736 T11732 advcl reveal,using
R7519 T11737 T11736 dobj functions,reveal
R7520 T11738 T11739 neg not,recapitulated
R7521 T11739 T11737 acl recapitulated,functions
R7522 T11740 T11739 advmod always,recapitulated
R7523 T11741 T11739 prep in,recapitulated
R7524 T11742 T11743 amod stable,models
R7525 T11743 T11741 pobj models,in
R7526 T11744 T11745 compound cell,line
R7527 T11745 T11743 compound line,models
R7528 T11746 T11722 punct .,be
R7529 T11748 T11749 amod Future,studies
R7530 T11749 T11750 nsubj studies,highlight
R7531 T11751 T11750 aux should,highlight
R7532 T11752 T11753 det the,reasons
R7533 T11753 T11750 dobj reasons,highlight
R7534 T11754 T11753 amod underlying,reasons
R7535 T11755 T11753 prep for,reasons
R7536 T11756 T11757 det these,results
R7537 T11757 T11755 pobj results,for
R7538 T11758 T11757 amod opposing,results
R7539 T11759 T11750 punct .,highlight
R7540 T11761 T11762 advcl Irrespective,stand
R7541 T11763 T11761 prep of,Irrespective
R7542 T11764 T11765 det these,differences
R7543 T11765 T11763 pobj differences,of
R7544 T11766 T11762 punct ", ",stand
R7545 T11767 T11768 poss our,studies
R7546 T11768 T11762 nsubj studies,stand
R7547 T11769 T11770 advmod in,vivo
R7548 T11770 T11768 amod vivo,studies
R7549 T11771 T11762 aux do,stand
R7550 T11772 T11762 neg not,stand
R7551 T11773 T11762 advmod alone,stand
R7552 T11774 T11762 punct ", ",stand
R7553 T11775 T11776 mark as,are
R7554 T11776 T11762 advcl are,stand
R7555 T11777 T11776 expl there,are
R7556 T11778 T11779 amod many,situations
R7557 T11779 T11776 attr situations,are
R7558 T11780 T11781 prep in,correlate
R7559 T11781 T11779 relcl correlate,situations
R7560 T11782 T11780 pobj which,in
R7561 T11783 T11784 det a,regulation
R7562 T11784 T11781 nsubj regulation,correlate
R7563 T11785 T11784 amod down,regulation
R7564 T11786 T11784 punct -,regulation
R7565 T11787 T11784 prep in,regulation
R7566 T11788 T11789 compound AJ,proteins
R7567 T11789 T11787 pobj proteins,in
R7568 T11790 T11781 prep with,correlate
R7569 T11791 T11792 amod enhanced,proliferation
R7570 T11792 T11790 pobj proliferation,with
R7571 T11793 T11762 punct .,stand
R7572 T11795 T11796 prep In,implicated
R7573 T11797 T11795 pobj fact,In
R7574 T11798 T11796 punct ", ",implicated
R7575 T11799 T11800 det a,myriad
R7576 T11800 T11796 nsubjpass myriad,implicated
R7577 T11801 T11800 prep of,myriad
R7578 T11802 T11803 amod diverse,mechanisms
R7579 T11803 T11801 pobj mechanisms,of
R7580 T11804 T11796 aux have,implicated
R7581 T11805 T11796 auxpass been,implicated
R7582 T11806 T11796 prep in,implicated
R7583 T11807 T11806 pcomp activating,in
R7584 T11808 T11809 amod epithelial,proliferation
R7585 T11809 T11807 dobj proliferation,activating
R7586 T11810 T11807 prep upon,activating
R7587 T11811 T11812 amod down,regulation
R7588 T11812 T11810 pobj regulation,upon
R7589 T11813 T11812 punct -,regulation
R7590 T11814 T11812 prep of,regulation
R7591 T11815 T11816 compound AJ,proteins
R7592 T11816 T11814 pobj proteins,of
R7593 T11817 T11818 punct [,48
R7594 T11818 T11796 parataxis 48,implicated
R7595 T11819 T11818 nummod 7,48
R7596 T11820 T11818 punct ",",48
R7597 T11821 T11818 nummod 23,48
R7598 T11822 T11818 punct ",",48
R7599 T11823 T11818 nummod 24,48
R7600 T11824 T11818 punct ",",48
R7601 T11825 T11818 punct ],48
R7602 T11826 T11796 punct .,implicated
R7603 T11828 T11829 csubj Sifting,is
R7604 T11830 T11828 prep through,Sifting
R7605 T11831 T11832 det these,pathways
R7606 T11832 T11830 pobj pathways,through
R7607 T11833 T11832 amod converging,pathways
R7608 T11834 T11829 acomp likely,is
R7609 T11835 T11836 aux to,be
R7610 T11836 T11834 xcomp be,likely
R7611 T11837 T11838 det a,process
R7612 T11838 T11836 attr process,be
R7613 T11839 T11838 amod difficult,process
R7614 T11840 T11839 cc and,difficult
R7615 T11841 T11839 conj painstaking,difficult
R7616 T11842 T11829 punct .,is
R7617 T11844 T11845 nsubj This,said
R7618 T11845 T11846 advcl said,begin
R7619 T11847 T11846 punct ", ",begin
R7620 T11848 T11846 prep by,begin
R7621 T11849 T11848 pcomp identifying,by
R7622 T11850 T11851 det the,status
R7623 T11851 T11849 dobj status,identifying
R7624 T11852 T11851 prep of,status
R7625 T11853 T11854 amod different,players
R7626 T11854 T11852 pobj players,of
R7627 T11855 T11854 acl involved,players
R7628 T11856 T11849 prep in,identifying
R7629 T11857 T11858 amod specific,types
R7630 T11858 T11856 pobj types,in
R7631 T11859 T11858 compound cell,types
R7632 T11860 T11856 cc and,in
R7633 T11861 T11856 conj at,in
R7634 T11862 T11863 amod specific,stages
R7635 T11863 T11861 pobj stages,at
R7636 T11864 T11863 prep in,stages
R7637 T11865 T11864 pobj development,in
R7638 T11866 T11846 punct ", ",begin
R7639 T11867 T11868 poss our,understanding
R7640 T11868 T11846 nsubj understanding,begin
R7641 T11869 T11868 amod mechanistic,understanding
R7642 T11870 T11868 prep of,understanding
R7643 T11871 T11872 advmod how,linked
R7644 T11872 T11870 pcomp linked,of
R7645 T11873 T11874 amod intercellular,remodeling
R7646 T11874 T11872 nsubjpass remodeling,linked
R7647 T11875 T11872 auxpass is,linked
R7648 T11876 T11872 prep to,linked
R7649 T11877 T11876 pobj proliferation,to
R7650 T11878 T11872 prep in,linked
R7651 T11879 T11880 amod epithelial,morphogenesis
R7652 T11880 T11878 pobj morphogenesis,in
R7653 T11881 T11846 aux should,begin
R7654 T11882 T11883 aux to,surface
R7655 T11883 T11846 xcomp surface,begin
R7656 T11884 T11883 prep in,surface
R7657 T11885 T11886 det the,future
R7658 T11886 T11884 pobj future,in
R7659 T11887 T11846 punct .,begin
R7660 T11889 T11890 csubj Elucidating,is
R7661 T11891 T11892 det the,mechanisms
R7662 T11892 T11889 dobj mechanisms,Elucidating
R7663 T11893 T11892 amod molecular,mechanisms
R7664 T11894 T11895 prep through,converge
R7665 T11895 T11892 relcl converge,mechanisms
R7666 T11896 T11894 pobj which,through
R7667 T11897 T11898 det these,networks
R7668 T11898 T11895 nsubj networks,converge
R7669 T11899 T11890 advmod also,is
R7670 T11900 T11901 det a,prerequisite
R7671 T11901 T11890 attr prerequisite,is
R7672 T11902 T11901 prep for,prerequisite
R7673 T11903 T11902 pcomp understanding,for
R7674 T11904 T11905 advmod how,go
R7675 T11905 T11903 ccomp go,understanding
R7676 T11906 T11907 det these,processes
R7677 T11907 T11905 nsubj processes,go
R7678 T11908 T11905 advmod awry,go
R7679 T11909 T11905 prep during,go
R7680 T11910 T11909 pobj tumorigenesis,during
R7681 T11911 T11890 punct .,is
R7682 T12023 T12024 amod Primary,antibodies
R7683 T12024 T12025 nsubj antibodies,were
R7684 T12026 T12024 acl used,antibodies
R7685 T12027 T12025 prep against,were
R7686 T12028 T12027 punct : ,against
R7687 T12029 T12030 compound E,cadherin
R7688 T12030 T12027 pobj cadherin,against
R7689 T12031 T12030 punct -,cadherin
R7690 T12032 T12033 punct (,Takeichi
R7691 T12033 T12030 parataxis Takeichi,cadherin
R7692 T12034 T12033 compound M.,Takeichi
R7693 T12035 T12033 punct ", ",Takeichi
R7694 T12036 T12037 compound Kyoto,University
R7695 T12037 T12033 npadvmod University,Takeichi
R7696 T12038 T12033 punct ", ",Takeichi
R7697 T12039 T12033 npadvmod Japan,Takeichi
R7698 T12040 T12033 punct ),Takeichi
R7699 T12041 T12030 punct ;,cadherin
R7700 T12042 T12043 compound α,catenin
R7701 T12043 T12030 conj catenin,cadherin
R7702 T12044 T12043 punct -,catenin
R7703 T12045 T12043 punct ", ",catenin
R7704 T12046 T12047 compound β,catenin
R7705 T12047 T12043 appos catenin,catenin
R7706 T12048 T12047 punct -,catenin
R7707 T12049 T12043 punct ", ",catenin
R7708 T12050 T12043 appos pMAPK,catenin
R7709 T12051 T12043 punct ", ",catenin
R7710 T12052 T12043 appos tubulin,catenin
R7711 T12053 T12054 punct (,Sigma
R7712 T12054 T12043 parataxis Sigma,catenin
R7713 T12055 T12054 punct ", ",Sigma
R7714 T12056 T12057 compound St.,Louis
R7715 T12057 T12054 npadvmod Louis,Sigma
R7716 T12058 T12054 punct ", ",Sigma
R7717 T12059 T12054 npadvmod Missouri,Sigma
R7718 T12060 T12054 punct ", ",Sigma
R7719 T12061 T12062 compound United,States
R7720 T12062 T12054 npadvmod States,Sigma
R7721 T12063 T12054 punct ),Sigma
R7722 T12064 T12043 punct ", ",catenin
R7723 T12065 T12043 conj Ajuba,catenin
R7724 T12066 T12067 punct (,Longmore
R7725 T12067 T12065 parataxis Longmore,Ajuba
R7726 T12068 T12067 compound G.,Longmore
R7727 T12069 T12067 punct ", ",Longmore
R7728 T12070 T12071 compound Washington,University
R7729 T12071 T12067 npadvmod University,Longmore
R7730 T12072 T12067 punct ", ",Longmore
R7731 T12073 T12074 compound St.,Louis
R7732 T12074 T12067 npadvmod Louis,Longmore
R7733 T12075 T12067 punct ", ",Longmore
R7734 T12076 T12067 npadvmod Missouri,Longmore
R7735 T12077 T12067 punct ", ",Longmore
R7736 T12078 T12079 compound United,States
R7737 T12079 T12067 npadvmod States,Longmore
R7738 T12080 T12067 punct ),Longmore
R7739 T12081 T12065 punct ;,Ajuba
R7740 T12082 T12083 compound β4,integrin
R7741 T12083 T12065 conj integrin,Ajuba
R7742 T12084 T12083 punct /,integrin
R7743 T12085 T12083 appos CD104,integrin
R7744 T12086 T12087 punct (,Pharmingen
R7745 T12087 T12083 parataxis Pharmingen,integrin
R7746 T12088 T12087 compound BD,Pharmingen
R7747 T12089 T12087 punct ", ",Pharmingen
R7748 T12090 T12091 compound San,Diego
R7749 T12091 T12087 npadvmod Diego,Pharmingen
R7750 T12092 T12087 punct ", ",Pharmingen
R7751 T12093 T12087 npadvmod California,Pharmingen
R7752 T12094 T12087 punct ", ",Pharmingen
R7753 T12095 T12096 compound United,States
R7754 T12096 T12087 npadvmod States,Pharmingen
R7755 T12097 T12087 punct ),Pharmingen
R7756 T12098 T12083 punct ", ",integrin
R7757 T12099 T12083 conj laminin,integrin
R7758 T12100 T12099 nummod 5,laminin
R7759 T12101 T12102 punct (,Burgeson
R7760 T12102 T12099 parataxis Burgeson,laminin
R7761 T12103 T12102 compound R.,Burgeson
R7762 T12104 T12102 punct ", ",Burgeson
R7763 T12105 T12106 compound Harvard,University
R7764 T12106 T12102 npadvmod University,Burgeson
R7765 T12107 T12102 punct ", ",Burgeson
R7766 T12108 T12102 npadvmod Cambridge,Burgeson
R7767 T12109 T12102 punct ", ",Burgeson
R7768 T12110 T12102 npadvmod Massachusetts,Burgeson
R7769 T12111 T12102 punct ", ",Burgeson
R7770 T12112 T12113 compound United,States
R7771 T12113 T12102 npadvmod States,Burgeson
R7772 T12114 T12102 punct ),Burgeson
R7773 T12115 T12099 punct ", ",laminin
R7774 T12116 T12099 conj K5,laminin
R7775 T12117 T12116 punct ", ",K5
R7776 T12118 T12116 conj K1,K5
R7777 T12119 T12118 punct ", ",K1
R7778 T12120 T12118 conj loricrin,K1
R7779 T12121 T12122 punct (,Lab
R7780 T12122 T12120 parataxis Lab,loricrin
R7781 T12123 T12122 compound Fuchs,Lab
R7782 T12124 T12122 punct ),Lab
R7783 T12125 T12120 punct ", ",loricrin
R7784 T12126 T12120 conj involucrin,loricrin
R7785 T12127 T12126 punct ", ",involucrin
R7786 T12128 T12126 conj fillagrin,involucrin
R7787 T12129 T12130 punct (,Covance
R7788 T12130 T12128 parataxis Covance,fillagrin
R7789 T12131 T12130 punct ", ",Covance
R7790 T12132 T12130 npadvmod Berkeley,Covance
R7791 T12133 T12130 punct ", ",Covance
R7792 T12134 T12130 npadvmod California,Covance
R7793 T12135 T12130 punct ", ",Covance
R7794 T12136 T12137 compound United,States
R7795 T12137 T12130 npadvmod States,Covance
R7796 T12138 T12130 punct ),Covance
R7797 T12139 T12128 punct ", ",fillagrin
R7798 T12140 T12128 conj MAPK,fillagrin
R7799 T12141 T12140 punct ", ",MAPK
R7800 T12142 T12140 conj pSMAD2,MAPK
R7801 T12143 T12144 punct (,Signaling
R7802 T12144 T12142 parataxis Signaling,pSMAD2
R7803 T12145 T12144 compound Cell,Signaling
R7804 T12146 T12144 punct ", ",Signaling
R7805 T12147 T12144 npadvmod Beverly,Signaling
R7806 T12148 T12144 punct ", ",Signaling
R7807 T12149 T12144 npadvmod Massachusetts,Signaling
R7808 T12150 T12144 punct ", ",Signaling
R7809 T12151 T12152 compound United,States
R7810 T12152 T12144 npadvmod States,Signaling
R7811 T12153 T12144 punct ),Signaling
R7812 T12154 T12142 punct ;,pSMAD2
R7813 T12155 T12142 conj Grb,pSMAD2
R7814 T12156 T12155 punct -,Grb
R7815 T12157 T12155 nummod 2,Grb
R7816 T12158 T12159 punct (,Biotech
R7817 T12159 T12155 parataxis Biotech,Grb
R7818 T12160 T12161 compound Santa,Cruz
R7819 T12161 T12159 compound Cruz,Biotech
R7820 T12162 T12159 punct ", ",Biotech
R7821 T12163 T12164 compound Santa,Cruz
R7822 T12164 T12159 npadvmod Cruz,Biotech
R7823 T12165 T12159 punct ", ",Biotech
R7824 T12166 T12159 npadvmod California,Biotech
R7825 T12167 T12159 punct ", ",Biotech
R7826 T12168 T12169 compound United,States
R7827 T12169 T12159 npadvmod States,Biotech
R7828 T12170 T12159 punct ),Biotech
R7829 T12171 T12155 punct ;,Grb
R7830 T12172 T12173 compound P,cadherin
R7831 T12173 T12155 conj cadherin,Grb
R7832 T12174 T12173 punct -,cadherin
R7833 T12175 T12176 punct (,Laboratories
R7834 T12176 T12173 parataxis Laboratories,cadherin
R7835 T12177 T12176 compound Zymed,Laboratories
R7836 T12178 T12176 punct ", ",Laboratories
R7837 T12179 T12180 amod South,Francisco
R7838 T12180 T12176 npadvmod Francisco,Laboratories
R7839 T12181 T12180 compound San,Francisco
R7840 T12182 T12176 punct ", ",Laboratories
R7841 T12183 T12176 npadvmod California,Laboratories
R7842 T12184 T12176 punct ", ",Laboratories
R7843 T12185 T12186 compound United,States
R7844 T12186 T12176 npadvmod States,Laboratories
R7845 T12187 T12176 punct ),Laboratories
R7846 T12188 T12173 punct ;,cadherin
R7847 T12189 T12173 conj HA,cadherin
R7848 T12190 T12191 punct (,Biochemicals
R7849 T12191 T12189 parataxis Biochemicals,HA
R7850 T12192 T12191 compound Roche,Biochemicals
R7851 T12193 T12191 punct ),Biochemicals
R7852 T12194 T12189 punct ", ",HA
R7853 T12195 T12189 conj vimentin,HA
R7854 T12196 T12197 punct (,Chemicon
R7855 T12197 T12195 parataxis Chemicon,vimentin
R7856 T12198 T12197 punct ", ",Chemicon
R7857 T12199 T12197 npadvmod Temecula,Chemicon
R7858 T12200 T12197 punct ", ",Chemicon
R7859 T12201 T12197 npadvmod California,Chemicon
R7860 T12202 T12197 punct ", ",Chemicon
R7861 T12203 T12204 compound United,States
R7862 T12204 T12197 npadvmod States,Chemicon
R7863 T12205 T12197 punct ),Chemicon
R7864 T12206 T12195 punct ", ",vimentin
R7865 T12207 T12195 conj Ki67,vimentin
R7866 T12208 T12209 punct (,Castra
R7867 T12209 T12207 parataxis Castra,Ki67
R7868 T12210 T12209 compound Novo,Castra
R7869 T12211 T12209 punct ", ",Castra
R7870 T12212 T12209 npadvmod Newcastle,Castra
R7871 T12213 T12212 prep Upon,Newcastle
R7872 T12214 T12213 pobj Tyne,Upon
R7873 T12215 T12209 punct ", ",Castra
R7874 T12216 T12217 compound United,Kingdom
R7875 T12217 T12209 npadvmod Kingdom,Castra
R7876 T12218 T12209 punct ),Castra
R7877 T12219 T12207 punct ", ",Ki67
R7878 T12220 T12207 conj keratin,Ki67
R7879 T12221 T12220 nummod 6,keratin
R7880 T12222 T12223 punct (,Coulombe
R7881 T12223 T12220 parataxis Coulombe,keratin
R7882 T12224 T12223 compound P.,Coulombe
R7883 T12225 T12223 punct ", ",Coulombe
R7884 T12226 T12227 compound John,Hopkins
R7885 T12227 T12228 compound Hopkins,University
R7886 T12228 T12223 npadvmod University,Coulombe
R7887 T12229 T12223 punct ", ",Coulombe
R7888 T12230 T12223 npadvmod Baltimore,Coulombe
R7889 T12231 T12223 punct ", ",Coulombe
R7890 T12232 T12223 npadvmod Maryland,Coulombe
R7891 T12233 T12223 punct ", ",Coulombe
R7892 T12234 T12235 compound United,States
R7893 T12235 T12223 npadvmod States,Coulombe
R7894 T12236 T12223 punct ),Coulombe
R7895 T12237 T12220 punct ", ",keratin
R7896 T12238 T12239 compound cyclin,D
R7897 T12239 T12220 conj D,keratin
R7898 T12240 T12241 punct (,Oncogene
R7899 T12241 T12239 parataxis Oncogene,D
R7900 T12242 T12241 punct ", ",Oncogene
R7901 T12243 T12244 compound San,Diego
R7902 T12244 T12241 npadvmod Diego,Oncogene
R7903 T12245 T12241 punct ", ",Oncogene
R7904 T12246 T12241 npadvmod California,Oncogene
R7905 T12247 T12241 punct ", ",Oncogene
R7906 T12248 T12249 compound United,States
R7907 T12249 T12241 npadvmod States,Oncogene
R7908 T12250 T12241 punct ),Oncogene
R7909 T12251 T12239 punct ", ",D
R7910 T12252 T12239 cc and,D
R7911 T12253 T12254 compound TGF,β2
R7912 T12254 T12239 conj β2,D
R7913 T12255 T12254 punct -,β2
R7914 T12256 T12257 punct (,Gold
R7915 T12257 T12254 parataxis Gold,β2
R7916 T12258 T12257 compound L.,Gold
R7917 T12259 T12257 punct ", ",Gold
R7918 T12260 T12261 compound New,York
R7919 T12261 T12262 compound York,University
R7920 T12262 T12257 npadvmod University,Gold
R7921 T12263 T12257 punct ", ",Gold
R7922 T12264 T12265 compound New,York
R7923 T12265 T12257 npadvmod York,Gold
R7924 T12266 T12257 punct ", ",Gold
R7925 T12267 T12268 compound New,York
R7926 T12268 T12257 npadvmod York,Gold
R7927 T12269 T12257 punct ", ",Gold
R7928 T12270 T12271 compound United,States
R7929 T12271 T12257 npadvmod States,Gold
R7930 T12272 T12257 punct ),Gold
R7931 T12273 T12025 punct .,were
R7932 T12275 T12276 npadvmod FITC,conjugated
R7933 T12276 T12286 amod conjugated,antibodies
R7934 T12277 T12275 punct -,FITC
R7935 T12278 T12275 punct ", ",FITC
R7936 T12279 T12280 compound Texas,Red
R7937 T12280 T12275 conj Red,FITC
R7938 T12281 T12280 punct -,Red
R7939 T12282 T12280 punct ", ",Red
R7940 T12283 T12280 cc or,Red
R7941 T12284 T12280 conj HRP,Red
R7942 T12285 T12276 punct -,conjugated
R7943 T12286 T12288 nsubj antibodies,were
R7944 T12287 T12286 amod secondary,antibodies
R7945 T12289 T12288 prep from,were
R7946 T12290 T12291 compound Jackson,ImmunoResearch
R7947 T12291 T12289 pobj ImmunoResearch,from
R7948 T12292 T12293 punct (,Grove
R7949 T12293 T12291 parataxis Grove,ImmunoResearch
R7950 T12294 T12293 compound West,Grove
R7951 T12295 T12293 punct ", ",Grove
R7952 T12296 T12293 npadvmod Pennsylvania,Grove
R7953 T12297 T12293 punct ", ",Grove
R7954 T12298 T12299 compound United,States
R7955 T12299 T12293 npadvmod States,Grove
R7956 T12300 T12293 punct ),Grove
R7957 T12301 T12288 punct .,were
R7958 T12303 T12304 amod Biotinylated,antibodies
R7959 T12304 T12306 nsubj antibodies,were
R7960 T12305 T12304 amod secondary,antibodies
R7961 T12307 T12306 prep from,were
R7962 T12308 T12309 compound Vector,Labs
R7963 T12309 T12307 pobj Labs,from
R7964 T12310 T12311 punct (,Burlingame
R7965 T12311 T12309 parataxis Burlingame,Labs
R7966 T12312 T12311 punct ", ",Burlingame
R7967 T12313 T12311 npadvmod California,Burlingame
R7968 T12314 T12311 punct ", ",Burlingame
R7969 T12315 T12316 compound United,States
R7970 T12316 T12311 npadvmod States,Burlingame
R7971 T12317 T12311 punct ),Burlingame
R7972 T12318 T12306 punct .,were
R7973 T12320 T12321 nsubj Dilutions,were
R7974 T12322 T12321 prep according,were
R7975 T12323 T12322 prep to,according
R7976 T12324 T12325 det the,manufacturer
R7977 T12325 T12326 poss manufacturer,recommendation
R7978 T12326 T12323 pobj recommendation,to
R7979 T12327 T12325 case 's,manufacturer
R7980 T12328 T12321 punct .,were
R7981 T12330 T12331 det The,antibody
R7982 T12331 T12333 nsubjpass antibody,generated
R7983 T12332 T12331 compound Snail,antibody
R7984 T12334 T12333 auxpass was,generated
R7985 T12335 T12333 prep in,generated
R7986 T12336 T12337 compound Guinea,pigs
R7987 T12337 T12335 pobj pigs,in
R7988 T12338 T12333 prep by,generated
R7989 T12339 T12338 pcomp inoculating,by
R7990 T12340 T12339 dobj them,inoculating
R7991 T12341 T12339 prep with,inoculating
R7992 T12342 T12343 det the,sequence
R7993 T12343 T12341 pobj sequence,with
R7994 T12344 T12345 npadvmod N,terminal
R7995 T12345 T12343 amod terminal,sequence
R7996 T12346 T12345 punct -,terminal
R7997 T12347 T12343 prep of,sequence
R7998 T12348 T12349 amod murine,Snail
R7999 T12349 T12347 pobj Snail,of
R8000 T12350 T12343 acl fused,sequence
R8001 T12351 T12350 prep to,fused
R8002 T12352 T12351 pobj GST,to
R8003 T12353 T12354 punct (,Covance
R8004 T12354 T12352 parataxis Covance,GST
R8005 T12355 T12354 punct ", ",Covance
R8006 T12356 T12354 npadvmod Princeton,Covance
R8007 T12357 T12354 punct ", ",Covance
R8008 T12358 T12359 compound New,Jersey
R8009 T12359 T12354 npadvmod Jersey,Covance
R8010 T12360 T12354 punct ", ",Covance
R8011 T12361 T12362 compound United,States
R8012 T12362 T12354 npadvmod States,Covance
R8013 T12363 T12354 punct ),Covance
R8014 T12364 T12333 punct .,generated
R8015 T12366 T12367 amod Recombinant,β2
R8016 T12367 T12371 nsubjpass β2,purchased
R8017 T12368 T12367 amod human,β2
R8018 T12369 T12367 compound TGF,β2
R8019 T12370 T12367 punct -,β2
R8020 T12372 T12371 auxpass was,purchased
R8021 T12373 T12371 prep from,purchased
R8022 T12374 T12373 pobj R,from
R8023 T12375 T12374 cc &,R
R8024 T12376 T12374 conj D,R
R8025 T12377 T12378 punct (,Minneapolis
R8026 T12378 T12376 parataxis Minneapolis,D
R8027 T12379 T12378 punct ", ",Minneapolis
R8028 T12380 T12378 npadvmod Minnesota,Minneapolis
R8029 T12381 T12378 punct ", ",Minneapolis
R8030 T12382 T12383 compound United,States
R8031 T12383 T12378 npadvmod States,Minneapolis
R8032 T12384 T12378 punct ),Minneapolis
R8033 T12385 T12371 punct .,purchased
R8034 T12387 T12388 npadvmod Heat,inactivated
R8035 T12388 T12389 amod inactivated,β2
R8036 T12389 T12392 nsubjpass β2,generated
R8037 T12390 T12389 compound TGF,β2
R8038 T12391 T12389 punct -,β2
R8039 T12393 T12392 auxpass was,generated
R8040 T12394 T12392 prep by,generated
R8041 T12395 T12394 pcomp heating,by
R8042 T12396 T12397 det the,protein
R8043 T12397 T12395 dobj protein,heating
R8044 T12398 T12397 amod recombinant,protein
R8045 T12399 T12395 prep at,heating
R8046 T12400 T12401 nummod 100,°C
R8047 T12401 T12399 pobj °C,at
R8048 T12402 T12395 prep for,heating
R8049 T12403 T12404 nummod 10,min
R8050 T12404 T12402 pobj min,for
R8051 T12405 T12392 punct .,generated
R8052 T12466 T12467 det The,mouse
R8053 T12467 T12472 nsubjpass mouse,generated
R8054 T12468 T12469 compound K14,Snail
R8055 T12469 T12467 compound Snail,mouse
R8056 T12470 T12469 punct -,Snail
R8057 T12471 T12467 compound Tg,mouse
R8058 T12473 T12472 auxpass was,generated
R8059 T12474 T12472 prep by,generated
R8060 T12475 T12474 pcomp digesting,by
R8061 T12476 T12477 det the,plasmid
R8062 T12477 T12475 dobj plasmid,digesting
R8063 T12478 T12479 compound pcDNA3,mm
R8064 T12479 T12477 compound mm,plasmid
R8065 T12480 T12479 punct -,mm
R8066 T12481 T12482 compound Snail,HA
R8067 T12482 T12477 compound HA,plasmid
R8068 T12483 T12482 punct -,HA
R8069 T12484 T12485 punct (,Herreros
R8070 T12485 T12477 parataxis Herreros,plasmid
R8071 T12486 T12485 compound G.,Herreros
R8072 T12487 T12485 compound de,Herreros
R8073 T12488 T12485 punct ", ",Herreros
R8074 T12489 T12490 compound Universitat,Pompeu
R8075 T12490 T12485 npadvmod Pompeu,Herreros
R8076 T12491 T12485 punct ", ",Herreros
R8077 T12492 T12485 npadvmod Fabra,Herreros
R8078 T12493 T12485 punct ", ",Herreros
R8079 T12494 T12485 npadvmod Barcelona,Herreros
R8080 T12495 T12485 punct ", ",Herreros
R8081 T12496 T12485 npadvmod Spain,Herreros
R8082 T12497 T12485 punct ),Herreros
R8083 T12498 T12475 prep with,digesting
R8084 T12499 T12498 pobj BamHI,with
R8085 T12500 T12499 cc and,BamHI
R8086 T12501 T12499 conj NotI,BamHI
R8087 T12502 T12472 cc and,generated
R8088 T12503 T12472 conj subcloned,generated
R8089 T12504 T12503 prep into,subcloned
R8090 T12505 T12506 det the,vector
R8091 T12506 T12504 pobj vector,into
R8092 T12507 T12506 compound K14,vector
R8093 T12508 T12509 punct [,49
R8094 T12509 T12503 parataxis 49,subcloned
R8095 T12510 T12509 punct ],49
R8096 T12511 T12472 punct .,generated
R8097 T12513 T12514 det The,construct
R8098 T12514 T12516 nsubjpass construct,injected
R8099 T12515 T12514 amod linearized,construct
R8100 T12517 T12516 auxpass was,injected
R8101 T12518 T12516 prep into,injected
R8102 T12519 T12520 det the,nucleus
R8103 T12520 T12518 pobj nucleus,into
R8104 T12521 T12520 prep of,nucleus
R8105 T12522 T12521 pobj embryos,of
R8106 T12523 T12522 prep from,embryos
R8107 T12524 T12525 compound CD1,mice
R8108 T12525 T12523 pobj mice,from
R8109 T12526 T12516 punct .,injected
R8110 T12528 T12529 det The,mouse
R8111 T12529 T12535 nsubjpass mouse,reported
R8112 T12530 T12531 nmod K14,Smad
R8113 T12531 T12529 nmod Smad,mouse
R8114 T12532 T12531 punct -,Smad
R8115 T12533 T12534 nummod 2,Tg
R8116 T12534 T12529 compound Tg,mouse
R8117 T12536 T12535 auxpass was,reported
R8118 T12537 T12535 prep in,reported
R8119 T12538 T12537 pobj Ito,in
R8120 T12539 T12540 advmod et,al.
R8121 T12540 T12538 advmod al.,Ito
R8122 T12541 T12538 punct ", ",Ito
R8123 T12542 T12538 npadvmod 2001,Ito
R8124 T12543 T12535 punct .,reported
R8125 T12545 T12546 det The,mouse
R8126 T12546 T12554 nsubjpass mouse,described
R8127 T12547 T12548 nmod TGF,β2
R8128 T12548 T12546 nmod β2,mouse
R8129 T12549 T12548 punct -,β2
R8130 T12550 T12548 appos knockout,β2
R8131 T12551 T12550 punct (,knockout
R8132 T12552 T12550 appos KO,knockout
R8133 T12553 T12546 punct ),mouse
R8134 T12555 T12554 auxpass was,described
R8135 T12556 T12554 prep in,described
R8136 T12557 T12558 punct [,34
R8137 T12558 T12556 pobj 34,in
R8138 T12559 T12554 punct ],described
R8139 T12560 T12554 punct .,described
R8140 T12562 T12563 det The,mouse
R8141 T12563 T12566 nsubjpass mouse,reported
R8142 T12564 T12565 compound shh,KO
R8143 T12565 T12563 compound KO,mouse
R8144 T12567 T12568 punct [,38
R8145 T12568 T12563 parataxis 38,mouse
R8146 T12569 T12568 punct ],38
R8147 T12570 T12563 cc and,mouse
R8148 T12571 T12572 compound TOPGal,mouse
R8149 T12572 T12563 conj mouse,mouse
R8150 T12573 T12574 punct [,20
R8151 T12574 T12572 parataxis 20,mouse
R8152 T12575 T12574 punct ],20
R8153 T12576 T12566 aux have,reported
R8154 T12577 T12566 advmod previously,reported
R8155 T12578 T12566 auxpass been,reported
R8156 T12579 T12566 punct .,reported
R8157 T12687 T12688 compound Western,blot
R8158 T12689 T12688 cc and,blot
R8159 T12690 T12688 conj immunoprecipitation,blot
R8160 T12692 T12693 compound Protein,extracts
R8161 T12693 T12694 nsubjpass extracts,generated
R8162 T12695 T12693 prep from,extracts
R8163 T12696 T12697 amod primary,keratinocytes
R8164 T12697 T12695 pobj keratinocytes,from
R8165 T12698 T12694 auxpass were,generated
R8166 T12699 T12700 preconj either,by
R8167 T12700 T12694 prep by,generated
R8168 T12701 T12700 pcomp lysing,by
R8169 T12702 T12701 dobj cells,lysing
R8170 T12703 T12701 prep in,lysing
R8171 T12704 T12705 compound lysis,buffer
R8172 T12705 T12703 pobj buffer,in
R8173 T12706 T12707 punct (,NP
R8174 T12707 T12705 parataxis NP,buffer
R8175 T12708 T12709 nummod 1,%
R8176 T12709 T12707 compound %,NP
R8177 T12710 T12707 punct -,NP
R8178 T12711 T12707 nummod 40,NP
R8179 T12712 T12707 punct ", ",NP
R8180 T12713 T12714 nummod 1,%
R8181 T12714 T12715 compound %,deoxycholate
R8182 T12715 T12707 conj deoxycholate,NP
R8183 T12716 T12715 compound sodium,deoxycholate
R8184 T12717 T12715 punct ", ",deoxycholate
R8185 T12718 T12719 nummod 20,mM
R8186 T12719 T12720 compound mM,Cl
R8187 T12720 T12715 conj Cl,deoxycholate
R8188 T12721 T12720 compound Tris,Cl
R8189 T12722 T12720 punct -,Cl
R8190 T12723 T12720 punct [,Cl
R8191 T12724 T12720 npadvmod pH,Cl
R8192 T12725 T12724 nummod 7.4,pH
R8193 T12726 T12720 punct ],Cl
R8194 T12727 T12720 punct ", ",Cl
R8195 T12728 T12729 nummod 140,mM
R8196 T12729 T12730 compound mM,NaCl
R8197 T12730 T12720 conj NaCl,Cl
R8198 T12731 T12730 acl containing,NaCl
R8199 T12732 T12733 nummod 1,mM
R8200 T12733 T12734 compound mM,vanadate
R8201 T12734 T12731 dobj vanadate,containing
R8202 T12735 T12734 compound sodium,vanadate
R8203 T12736 T12730 punct ", ",NaCl
R8204 T12737 T12738 nummod 2,mM
R8205 T12738 T12739 compound mM,fluoride
R8206 T12739 T12730 conj fluoride,NaCl
R8207 T12740 T12739 compound phenylmethylsulfonyl,fluoride
R8208 T12741 T12739 punct ", ",fluoride
R8209 T12742 T12739 cc and,fluoride
R8210 T12743 T12744 compound protease,inhibitors
R8211 T12744 T12739 conj inhibitors,fluoride
R8212 T12745 T12707 punct ),NP
R8213 T12746 T12703 cc or,in
R8214 T12747 T12748 advmod directly,in
R8215 T12748 T12703 conj in,in
R8216 T12749 T12750 compound Laemmli,bβuffer
R8217 T12750 T12748 pobj bβuffer,in
R8218 T12751 T12694 cc and,generated
R8219 T12752 T12694 conj boiled,generated
R8220 T12753 T12694 punct .,generated
R8221 T12755 T12756 prep For,pulverized
R8222 T12757 T12758 compound skin,tissue
R8223 T12758 T12755 pobj tissue,For
R8224 T12759 T12756 punct : ,pulverized
R8225 T12760 T12761 amod Frozen,tissue
R8226 T12761 T12756 nsubjpass tissue,pulverized
R8227 T12762 T12756 auxpass was,pulverized
R8228 T12763 T12756 prep in,pulverized
R8229 T12764 T12765 det a,Gevebesmascher
R8230 T12765 T12763 pobj Gevebesmascher,in
R8231 T12766 T12767 amod liquid,nitrogen
R8232 T12767 T12768 npadvmod nitrogen,cooled
R8233 T12768 T12765 amod cooled,Gevebesmascher
R8234 T12769 T12768 punct -,cooled
R8235 T12770 T12756 cc and,pulverized
R8236 T12771 T12772 det the,powder
R8237 T12772 T12773 nsubj powder,scraped
R8238 T12773 T12756 conj scraped,pulverized
R8239 T12774 T12773 prep into,scraped
R8240 T12775 T12776 det a,tube
R8241 T12776 T12774 pobj tube,into
R8242 T12777 T12776 amod chilled,tube
R8243 T12778 T12776 compound microcentrifuge,tube
R8244 T12779 T12756 punct .,pulverized
R8245 T12781 T12782 compound RIPA,buffer
R8246 T12782 T12783 nsubjpass buffer,added
R8247 T12784 T12785 punct (,X
R8248 T12785 T12782 parataxis X,buffer
R8249 T12786 T12787 nummod 1,%
R8250 T12787 T12785 compound %,X
R8251 T12788 T12785 compound Triton,X
R8252 T12789 T12785 punct -,X
R8253 T12790 T12785 nummod 100,X
R8254 T12791 T12785 prep in,X
R8255 T12792 T12791 pobj PBS,in
R8256 T12793 T12785 prep with,X
R8257 T12794 T12795 nummod 10,mM
R8258 T12795 T12796 compound mM,EDTA
R8259 T12796 T12793 pobj EDTA,with
R8260 T12797 T12796 punct ", ",EDTA
R8261 T12798 T12799 nummod 150,mN
R8262 T12799 T12800 compound mN,NaCl
R8263 T12800 T12796 conj NaCl,EDTA
R8264 T12801 T12800 punct ", ",NaCl
R8265 T12802 T12803 nummod 1,%
R8266 T12803 T12804 compound %,deoxycholate
R8267 T12804 T12800 conj deoxycholate,NaCl
R8268 T12805 T12804 compound sodium,deoxycholate
R8269 T12806 T12804 punct ", ",deoxycholate
R8270 T12807 T12804 cc and,deoxycholate
R8271 T12808 T12809 nummod 0.1,%
R8272 T12809 T12810 compound %,SDS
R8273 T12810 T12804 conj SDS,deoxycholate
R8274 T12811 T12785 punct ),X
R8275 T12812 T12782 cc and,buffer
R8276 T12813 T12814 compound protease,inhibitors
R8277 T12814 T12782 conj inhibitors,buffer
R8278 T12815 T12814 cc or,inhibitors
R8279 T12816 T12817 compound Laemmli,buffer
R8280 T12817 T12814 conj buffer,inhibitors
R8281 T12818 T12783 auxpass was,added
R8282 T12819 T12783 punct .,added
R8283 T12821 T12822 det The,suspension
R8284 T12822 T12824 nsubjpass suspension,sonicated
R8285 T12823 T12822 compound cell,suspension
R8286 T12825 T12824 auxpass was,sonicated
R8287 T12826 T12827 nummod three,times
R8288 T12827 T12824 npadvmod times,sonicated
R8289 T12828 T12824 prep for,sonicated
R8290 T12829 T12830 nummod 15,s
R8291 T12830 T12828 pobj s,for
R8292 T12831 T12824 cc and,sonicated
R8293 T12832 T12824 conj centrifuged,sonicated
R8294 T12833 T12832 prep at,centrifuged
R8295 T12834 T12835 nummod "14,000",rpm
R8296 T12835 T12833 pobj rpm,at
R8297 T12836 T12832 prep at,centrifuged
R8298 T12837 T12838 nummod 4,°C
R8299 T12838 T12836 pobj °C,at
R8300 T12839 T12824 punct .,sonicated
R8301 T12841 T12842 det The,supernatant
R8302 T12842 T12843 nsubjpass supernatant,separated
R8303 T12844 T12843 auxpass was,separated
R8304 T12845 T12843 prep from,separated
R8305 T12846 T12847 det the,pellet
R8306 T12847 T12845 pobj pellet,from
R8307 T12848 T12843 cc and,separated
R8308 T12849 T12843 conj used,separated
R8309 T12850 T12849 prep in,used
R8310 T12851 T12852 det the,experiments
R8311 T12852 T12850 pobj experiments,in
R8312 T12853 T12843 punct .,separated
R8313 T12855 T12856 nsubjpass Extracts,precleared
R8314 T12857 T12855 acl subjected,Extracts
R8315 T12858 T12857 prep to,subjected
R8316 T12859 T12858 pobj immunoprecipitation,to
R8317 T12860 T12856 auxpass were,precleared
R8318 T12861 T12856 prep with,precleared
R8319 T12862 T12863 compound Protein,G
R8320 T12863 T12864 compound G,Sepharose
R8321 T12864 T12861 pobj Sepharose,with
R8322 T12865 T12866 punct (,Amersham
R8323 T12866 T12864 parataxis Amersham,Sepharose
R8324 T12867 T12866 punct ", ",Amersham
R8325 T12868 T12866 npadvmod Piscataway,Amersham
R8326 T12869 T12866 punct ", ",Amersham
R8327 T12870 T12871 compound New,York
R8328 T12871 T12866 npadvmod York,Amersham
R8329 T12872 T12866 punct ", ",Amersham
R8330 T12873 T12874 compound United,States
R8331 T12874 T12866 npadvmod States,Amersham
R8332 T12875 T12866 punct ),Amersham
R8333 T12876 T12856 cc and,precleared
R8334 T12877 T12856 conj incubated,precleared
R8335 T12878 T12877 prep with,incubated
R8336 T12879 T12878 pobj antibody,with
R8337 T12880 T12877 prep with,incubated
R8338 T12881 T12880 pobj rocking,with
R8339 T12882 T12877 advmod overnight,incubated
R8340 T12883 T12877 prep at,incubated
R8341 T12884 T12885 nummod 4,°C
R8342 T12885 T12883 pobj °C,at
R8343 T12886 T12856 punct .,precleared
R8344 T12888 T12889 compound Protein,G
R8345 T12889 T12890 compound G,Sepharose
R8346 T12890 T12891 nsubjpass Sepharose,added
R8347 T12892 T12891 auxpass was,added
R8348 T12893 T12891 cc and,added
R8349 T12894 T12895 nsubjpass samples,incubated
R8350 T12895 T12891 conj incubated,added
R8351 T12896 T12895 auxpass were,incubated
R8352 T12897 T12895 prep for,incubated
R8353 T12898 T12899 nummod 1,h
R8354 T12899 T12897 pobj h,for
R8355 T12900 T12895 prep at,incubated
R8356 T12901 T12902 nummod 4,°C
R8357 T12902 T12900 pobj °C,at
R8358 T12903 T12895 prep with,incubated
R8359 T12904 T12903 pobj rocking,with
R8360 T12905 T12895 punct .,incubated
R8361 T12907 T12908 nsubjpass Samples,washed
R8362 T12909 T12908 auxpass were,washed
R8363 T12910 T12911 nummod three,times
R8364 T12911 T12908 npadvmod times,washed
R8365 T12912 T12908 prep for,washed
R8366 T12913 T12914 nummod 5,min
R8367 T12914 T12912 pobj min,for
R8368 T12915 T12914 advmod each,min
R8369 T12916 T12908 prep in,washed
R8370 T12917 T12918 compound lysis,buffer
R8371 T12918 T12916 pobj buffer,in
R8372 T12919 T12908 punct ", ",washed
R8373 T12920 T12908 cc and,washed
R8374 T12921 T12922 det the,pellet
R8375 T12922 T12930 nsubjpass pellet,resuspended
R8376 T12923 T12924 compound Protein,G
R8377 T12924 T12925 compound G,antigen
R8378 T12925 T12922 compound antigen,pellet
R8379 T12926 T12925 compound Sepharose,antigen
R8380 T12927 T12925 punct -,antigen
R8381 T12928 T12925 compound antibody,antigen
R8382 T12929 T12925 punct -,antigen
R8383 T12930 T12908 conj resuspended,washed
R8384 T12931 T12930 auxpass was,resuspended
R8385 T12932 T12930 prep in,resuspended
R8386 T12933 T12934 compound Laemmli,buffer
R8387 T12934 T12932 pobj buffer,in
R8388 T12935 T12930 cc and,resuspended
R8389 T12936 T12930 conj boiled,resuspended
R8390 T12937 T12936 prep for,boiled
R8391 T12938 T12939 nummod 10,min
R8392 T12939 T12937 pobj min,for
R8393 T12940 T12930 punct .,resuspended
R8394 T12942 T12943 nsubjpass Samples,run
R8395 T12944 T12943 auxpass were,run
R8396 T12945 T12943 prep on,run
R8397 T12946 T12947 compound SDS,PAGE
R8398 T12947 T12945 pobj PAGE,on
R8399 T12948 T12947 punct -,PAGE
R8400 T12949 T12943 cc and,run
R8401 T12950 T12943 conj transferred,run
R8402 T12951 T12950 prep to,transferred
R8403 T12952 T12953 compound nitrocellulose,membrane
R8404 T12953 T12951 pobj membrane,to
R8405 T12954 T12955 punct (,Bioscience
R8406 T12955 T12953 parataxis Bioscience,membrane
R8407 T12956 T12955 nmod Schleicher,Bioscience
R8408 T12957 T12956 cc and,Schleicher
R8409 T12958 T12956 conj Schuell,Schleicher
R8410 T12959 T12955 punct ", ",Bioscience
R8411 T12960 T12955 npadvmod Keene,Bioscience
R8412 T12961 T12955 punct ", ",Bioscience
R8413 T12962 T12963 compound New,Hampshire
R8414 T12963 T12955 npadvmod Hampshire,Bioscience
R8415 T12964 T12955 punct ", ",Bioscience
R8416 T12965 T12966 compound United,States
R8417 T12966 T12955 npadvmod States,Bioscience
R8418 T12967 T12955 punct ),Bioscience
R8419 T12968 T12943 punct .,run
R8420 T12970 T12971 compound Western,blot
R8421 T12971 T12972 compound blot,signals
R8422 T12972 T12973 nsubjpass signals,developed
R8423 T12974 T12973 auxpass were,developed
R8424 T12975 T12973 advcl using,developed
R8425 T12976 T12977 det the,kit
R8426 T12977 T12975 dobj kit,using
R8427 T12978 T12977 amod enhanced,kit
R8428 T12979 T12977 compound chemiluminescence,kit
R8429 T12980 T12977 prep from,kit
R8430 T12981 T12980 pobj Amersham,from
R8431 T13041 T13042 compound Cell,culture
R8432 T13044 T13045 amod Primary,keratinocytes
R8433 T13045 T13046 nsubjpass keratinocytes,culture
R8434 T13047 T13046 auxpass were,culture
R8435 T13048 T13046 prep in,culture
R8436 T13049 T13050 amod low,calcium
R8437 T13050 T13052 compound calcium,medium
R8438 T13051 T13050 punct -,calcium
R8439 T13052 T13048 pobj medium,in
R8440 T13053 T13054 mark as,described
R8441 T13054 T13046 advcl described,culture
R8442 T13055 T13054 advmod previously,described
R8443 T13056 T13057 punct [,4
R8444 T13057 T13046 parataxis 4,culture
R8445 T13058 T13057 punct ],4
R8446 T13059 T13046 punct .,culture
R8447 T13061 T13062 amod Transient,transfections
R8448 T13062 T13063 nsubjpass transfections,carried
R8449 T13064 T13063 auxpass were,carried
R8450 T13065 T13063 prt out,carried
R8451 T13066 T13063 prep with,carried
R8452 T13067 T13068 compound FuGENE6,reagent
R8453 T13068 T13066 pobj reagent,with
R8454 T13069 T13070 punct (,Roche
R8455 T13070 T13068 parataxis Roche,reagent
R8456 T13071 T13070 punct ", ",Roche
R8457 T13072 T13070 npadvmod Indianapolis,Roche
R8458 T13073 T13070 punct ", ",Roche
R8459 T13074 T13070 npadvmod Indiana,Roche
R8460 T13075 T13070 punct ", ",Roche
R8461 T13076 T13077 compound United,States
R8462 T13077 T13070 npadvmod States,Roche
R8463 T13078 T13070 punct ),Roche
R8464 T13079 T13063 prep according,carried
R8465 T13080 T13079 prep to,according
R8466 T13081 T13082 det the,manufacturer
R8467 T13082 T13083 poss manufacturer,protocol
R8468 T13083 T13080 pobj protocol,to
R8469 T13084 T13082 case 's,manufacturer
R8470 T13085 T13063 punct .,carried
R8471 T13087 T13088 nsubjpass Measurement,carried
R8472 T13089 T13087 prep of,Measurement
R8473 T13090 T13091 nmod β,galactosidase
R8474 T13091 T13093 nmod galactosidase,levels
R8475 T13092 T13091 punct -,galactosidase
R8476 T13093 T13089 pobj levels,of
R8477 T13094 T13091 cc or,galactosidase
R8478 T13095 T13091 conj luciferase,galactosidase
R8479 T13096 T13088 prep in,carried
R8480 T13097 T13098 compound promoter,activity
R8481 T13098 T13099 compound activity,studies
R8482 T13099 T13096 pobj studies,in
R8483 T13100 T13088 auxpass were,carried
R8484 T13101 T13088 prt out,carried
R8485 T13102 T13088 prep with,carried
R8486 T13103 T13104 det the,kit
R8487 T13104 T13102 pobj kit,with
R8488 T13105 T13106 compound Galacto,Lite
R8489 T13106 T13104 compound Lite,kit
R8490 T13107 T13106 punct -,Lite
R8491 T13108 T13104 compound assay,kit
R8492 T13109 T13110 punct (,TROPIX
R8493 T13110 T13104 parataxis TROPIX,kit
R8494 T13111 T13110 punct ", ",TROPIX
R8495 T13112 T13110 npadvmod Bedford,TROPIX
R8496 T13113 T13110 punct ", ",TROPIX
R8497 T13114 T13110 npadvmod Massachusetts,TROPIX
R8498 T13115 T13110 punct ", ",TROPIX
R8499 T13116 T13117 compound United,States
R8500 T13117 T13110 npadvmod States,TROPIX
R8501 T13118 T13110 punct ),TROPIX
R8502 T13119 T13104 cc and,kit
R8503 T13120 T13121 det the,luciferase
R8504 T13121 T13104 conj luciferase,kit
R8505 T13122 T13121 amod Dual,luciferase
R8506 T13123 T13124 punct (,Promega
R8507 T13124 T13121 parataxis Promega,luciferase
R8508 T13125 T13124 punct ", ",Promega
R8509 T13126 T13124 npadvmod Madison,Promega
R8510 T13127 T13124 punct ", ",Promega
R8511 T13128 T13124 npadvmod Wisconsin,Promega
R8512 T13129 T13124 punct ", ",Promega
R8513 T13130 T13131 compound United,States
R8514 T13131 T13124 npadvmod States,Promega
R8515 T13132 T13124 punct ),Promega
R8516 T13133 T13121 punct ", ",luciferase
R8517 T13134 T13088 advmod respectively,carried
R8518 T13135 T13088 punct .,carried
R8519 T13137 T13138 compound Runella,luciferase
R8520 T13138 T13139 nsubjpass luciferase,cotransfected
R8521 T13140 T13139 auxpass was,cotransfected
R8522 T13141 T13139 prep into,cotransfected
R8523 T13142 T13141 pobj cells,into
R8524 T13143 T13144 aux to,correct
R8525 T13144 T13139 advcl correct,cotransfected
R8526 T13145 T13144 prep for,correct
R8527 T13146 T13147 compound transfection,efficiency
R8528 T13147 T13145 pobj efficiency,for
R8529 T13148 T13139 punct .,cotransfected
R8530 T13150 T13151 nsubjpass Experiments,done
R8531 T13152 T13151 auxpass were,done
R8532 T13153 T13151 prep in,done
R8533 T13154 T13153 pobj triplicate,in
R8534 T13155 T13151 cc and,done
R8535 T13156 T13151 conj repeated,done
R8536 T13157 T13158 advmod at,three
R8537 T13158 T13160 nummod three,times
R8538 T13159 T13158 advmod least,three
R8539 T13160 T13156 npadvmod times,repeated
R8540 T13161 T13151 punct .,done
R8541 T13163 T13164 nsubjpass Measurements,done
R8542 T13165 T13164 auxpass were,done
R8543 T13166 T13164 prep on,done
R8544 T13167 T13168 det a,luminometer
R8545 T13168 T13166 pobj luminometer,on
R8546 T13169 T13170 punct (,Instruments
R8547 T13170 T13168 parataxis Instruments,luminometer
R8548 T13171 T13170 compound MGM,Instruments
R8549 T13172 T13170 punct ", ",Instruments
R8550 T13173 T13170 npadvmod Hamden,Instruments
R8551 T13174 T13170 punct ", ",Instruments
R8552 T13175 T13170 npadvmod Connecticut,Instruments
R8553 T13176 T13170 punct ", ",Instruments
R8554 T13177 T13178 compound United,States
R8555 T13178 T13170 npadvmod States,Instruments
R8556 T13179 T13170 punct ),Instruments
R8557 T13180 T13164 punct .,done
R8558 T13182 T13183 prep For,starved
R8559 T13184 T13182 pobj experiments,For
R8560 T13185 T13184 acl measuring,experiments
R8561 T13186 T13185 dobj phosphorylation,measuring
R8562 T13187 T13186 prep of,phosphorylation
R8563 T13188 T13187 pobj MAPK,of
R8564 T13189 T13183 punct ", ",starved
R8565 T13190 T13183 nsubjpass keratinocytes,starved
R8566 T13191 T13183 auxpass were,starved
R8567 T13192 T13183 dep serum,starved
R8568 T13193 T13183 prep for,starved
R8569 T13194 T13195 nummod 3,h
R8570 T13195 T13193 pobj h,for
R8571 T13196 T13197 amod prior,to
R8572 T13197 T13183 prep to,starved
R8573 T13198 T13197 pobj harvesting,to
R8574 T13199 T13198 prep of,harvesting
R8575 T13200 T13199 pobj cells,of
R8576 T13201 T13198 prep by,harvesting
R8577 T13202 T13201 pobj incubation,by
R8578 T13203 T13202 prep in,incubation
R8579 T13204 T13203 pobj medium,in
R8580 T13205 T13204 acl lacking,medium
R8581 T13206 T13205 dobj serum,lacking
R8582 T13207 T13183 punct .,starved
R8583 T13209 T13210 nsubjpass Treatment,described
R8584 T13211 T13209 prep of,Treatment
R8585 T13212 T13211 pobj cells,of
R8586 T13213 T13209 prep with,Treatment
R8587 T13214 T13215 npadvmod Wnt,conditioned
R8588 T13215 T13220 amod conditioned,medium
R8589 T13216 T13214 punct -,Wnt
R8590 T13217 T13214 cc and,Wnt
R8591 T13218 T13214 conj noggin,Wnt
R8592 T13219 T13215 punct -,conditioned
R8593 T13220 T13213 pobj medium,with
R8594 T13221 T13210 auxpass was,described
R8595 T13222 T13210 advmod previously,described
R8596 T13223 T13224 punct [,4
R8597 T13224 T13210 parataxis 4,described
R8598 T13225 T13224 punct ],4
R8599 T13226 T13210 punct .,described
R8600 T13310 T13311 det The,promoter
R8601 T13311 T13317 nsubjpass promoter,generated
R8602 T13312 T13313 nummod 2.2,kb
R8603 T13313 T13311 nmod kb,promoter
R8604 T13314 T13313 punct -,kb
R8605 T13315 T13311 amod murine,promoter
R8606 T13316 T13311 compound Snail,promoter
R8607 T13318 T13317 auxpass was,generated
R8608 T13319 T13317 prep by,generated
R8609 T13320 T13319 pobj PCR,by
R8610 T13321 T13317 advcl using,generated
R8611 T13322 T13323 det a,primer
R8612 T13323 T13321 dobj primer,using
R8613 T13324 T13323 amod forward,primer
R8614 T13325 T13321 prep with,using
R8615 T13326 T13327 det an,sequence
R8616 T13327 T13325 pobj sequence,with
R8617 T13328 T13327 compound XbaI,sequence
R8618 T13329 T13327 compound linker,sequence
R8619 T13330 T13327 punct ", ",sequence
R8620 T13331 T13332 nummod 5,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8621 T13332 T13327 appos TCTAGAATTGTTTGCTGCTGTATGGTCTTC,sequence
R8622 T13333 T13331 punct ′,5
R8623 T13334 T13332 punct -,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8624 T13335 T13332 punct -,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8625 T13336 T13332 nummod 3,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8626 T13337 T13332 punct ′,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8627 T13338 T13327 punct ", ",sequence
R8628 T13339 T13327 cc along,sequence
R8629 T13340 T13339 dep with,along
R8630 T13341 T13342 det a,primer
R8631 T13342 T13327 conj primer,sequence
R8632 T13343 T13342 amod reverse,primer
R8633 T13344 T13342 prep with,primer
R8634 T13345 T13346 det a,sequence
R8635 T13346 T13344 pobj sequence,with
R8636 T13347 T13346 compound BglII,sequence
R8637 T13348 T13346 compound linker,sequence
R8638 T13349 T13346 punct ", ",sequence
R8639 T13350 T13351 nummod 5,GTAG
R8640 T13351 T13346 appos GTAG,sequence
R8641 T13352 T13350 punct ′,5
R8642 T13353 T13351 punct -,GTAG
R8643 T13354 T13351 compound AGATCTGTTGGCCAGAGCGACCTAG,GTAG
R8644 T13355 T13351 punct -,GTAG
R8645 T13356 T13351 punct -,GTAG
R8646 T13357 T13351 nummod 3,GTAG
R8647 T13358 T13351 punct ′,GTAG
R8648 T13359 T13342 punct ", ",primer
R8649 T13360 T13342 cc and,primer
R8650 T13361 T13362 nmod mouse,DNA
R8651 T13362 T13342 conj DNA,primer
R8652 T13363 T13362 amod genomic,DNA
R8653 T13364 T13362 prep as,DNA
R8654 T13365 T13366 det a,template
R8655 T13366 T13364 pobj template,as
R8656 T13367 T13317 punct .,generated
R8657 T13369 T13370 det The,product
R8658 T13370 T13372 nsubjpass product,purified
R8659 T13371 T13370 compound PCR,product
R8660 T13373 T13372 auxpass was,purified
R8661 T13374 T13372 prep with,purified
R8662 T13375 T13376 det the,Kit
R8663 T13376 T13374 pobj Kit,with
R8664 T13377 T13378 compound Gel,Extraction
R8665 T13378 T13376 compound Extraction,Kit
R8666 T13379 T13380 punct (,Qiagen
R8667 T13380 T13376 parataxis Qiagen,Kit
R8668 T13381 T13380 punct ", ",Qiagen
R8669 T13382 T13380 npadvmod Valencia,Qiagen
R8670 T13383 T13380 punct ", ",Qiagen
R8671 T13384 T13380 npadvmod California,Qiagen
R8672 T13385 T13380 punct ", ",Qiagen
R8673 T13386 T13387 compound United,States
R8674 T13387 T13380 npadvmod States,Qiagen
R8675 T13388 T13380 punct ),Qiagen
R8676 T13389 T13372 cc and,purified
R8677 T13390 T13372 conj ligated,purified
R8678 T13391 T13390 prep into,ligated
R8679 T13392 T13393 compound pCRII,TOPO
R8680 T13393 T13395 compound TOPO,vector
R8681 T13394 T13393 punct -,TOPO
R8682 T13395 T13391 pobj vector,into
R8683 T13396 T13395 compound TA,vector
R8684 T13397 T13398 punct (,Invitrogen
R8685 T13398 T13395 parataxis Invitrogen,vector
R8686 T13399 T13398 punct ", ",Invitrogen
R8687 T13400 T13398 npadvmod Carlsbad,Invitrogen
R8688 T13401 T13398 punct ", ",Invitrogen
R8689 T13402 T13398 npadvmod California,Invitrogen
R8690 T13403 T13398 punct ", ",Invitrogen
R8691 T13404 T13405 compound United,States
R8692 T13405 T13398 npadvmod States,Invitrogen
R8693 T13406 T13398 punct ),Invitrogen
R8694 T13407 T13372 punct .,purified
R8695 T13409 T13410 det The,promoter
R8696 T13410 T13411 nsubjpass promoter,verified
R8697 T13412 T13411 auxpass was,verified
R8698 T13413 T13411 prep by,verified
R8699 T13414 T13413 pobj sequencing,by
R8700 T13415 T13411 cc and,verified
R8701 T13416 T13411 conj digested,verified
R8702 T13417 T13416 prep with,digested
R8703 T13418 T13417 pobj XbaI,with
R8704 T13419 T13418 cc and,XbaI
R8705 T13420 T13418 conj BglII,XbaI
R8706 T13421 T13416 cc and,digested
R8707 T13422 T13416 conj subcloned,digested
R8708 T13423 T13422 prep into,subcloned
R8709 T13424 T13425 det the,vector
R8710 T13425 T13423 pobj vector,into
R8711 T13426 T13427 compound pβ,gal
R8712 T13427 T13425 compound gal,vector
R8713 T13428 T13427 punct -,gal
R8714 T13429 T13425 compound BASIC,vector
R8715 T13430 T13431 punct (,Clontech
R8716 T13431 T13425 parataxis Clontech,vector
R8717 T13432 T13431 compound BD,Clontech
R8718 T13433 T13431 compound Biosciences,Clontech
R8719 T13434 T13431 punct ", ",Clontech
R8720 T13435 T13436 compound Palo,Alto
R8721 T13436 T13431 npadvmod Alto,Clontech
R8722 T13437 T13431 punct ", ",Clontech
R8723 T13438 T13431 npadvmod California,Clontech
R8724 T13439 T13431 punct ", ",Clontech
R8725 T13440 T13441 compound United,States
R8726 T13441 T13431 npadvmod States,Clontech
R8727 T13442 T13431 punct ),Clontech
R8728 T13443 T13411 punct .,verified
R8729 T13445 T13446 det The,mutations
R8730 T13446 T13448 nsubjpass mutations,generated
R8731 T13447 T13446 compound point,mutations
R8732 T13449 T13446 prep in,mutations
R8733 T13450 T13451 det the,element
R8734 T13451 T13449 pobj element,in
R8735 T13452 T13451 compound SMAD,element
R8736 T13453 T13451 compound binding,element
R8737 T13454 T13448 auxpass was,generated
R8738 T13455 T13448 prep with,generated
R8739 T13456 T13457 det the,Kit
R8740 T13457 T13455 pobj Kit,with
R8741 T13458 T13459 compound Quik,Change
R8742 T13459 T13457 compound Change,Kit
R8743 T13460 T13459 punct -,Change
R8744 T13461 T13462 punct (,Stratagene
R8745 T13462 T13457 parataxis Stratagene,Kit
R8746 T13463 T13462 punct ", ",Stratagene
R8747 T13464 T13465 compound La,Jolla
R8748 T13465 T13462 npadvmod Jolla,Stratagene
R8749 T13466 T13462 punct ", ",Stratagene
R8750 T13467 T13462 npadvmod California,Stratagene
R8751 T13468 T13462 punct ", ",Stratagene
R8752 T13469 T13470 compound United,States
R8753 T13470 T13462 npadvmod States,Stratagene
R8754 T13471 T13462 punct ),Stratagene
R8755 T13472 T13448 advcl using,generated
R8756 T13473 T13474 det the,primer
R8757 T13474 T13472 dobj primer,using
R8758 T13475 T13474 amod forward,primer
R8759 T13476 T13477 nummod 5,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8760 T13477 T13474 appos GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC,primer
R8761 T13478 T13476 punct ′,5
R8762 T13479 T13477 punct -,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8763 T13480 T13477 punct -,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8764 T13481 T13477 nummod 3,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8765 T13482 T13477 punct ′,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8766 T13483 T13474 cc and,primer
R8767 T13484 T13485 det the,primer
R8768 T13485 T13474 conj primer,primer
R8769 T13486 T13485 amod reverse,primer
R8770 T13487 T13488 nummod 5,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8771 T13488 T13485 appos CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC,primer
R8772 T13489 T13487 punct ′,5
R8773 T13490 T13488 punct -,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8774 T13491 T13488 compound GCTTTT,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8775 T13492 T13488 punct -,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8776 T13493 T13488 punct -,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8777 T13494 T13488 nummod 3,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8778 T13495 T13488 punct ′,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8779 T13496 T13448 punct .,generated
R8780 T13498 T13499 det The,probes
R8781 T13499 T13500 nsubjpass probes,generated
R8782 T13501 T13499 prep for,probes
R8783 T13502 T13503 det the,hybridization
R8784 T13503 T13501 pobj hybridization,for
R8785 T13504 T13503 nmod Snail,hybridization
R8786 T13505 T13506 advmod in,situ
R8787 T13506 T13503 amod situ,hybridization
R8788 T13507 T13500 auxpass were,generated
R8789 T13508 T13500 prep against,generated
R8790 T13509 T13510 det the,UTR
R8791 T13510 T13508 pobj UTR,against
R8792 T13511 T13510 nummod 3,UTR
R8793 T13512 T13511 punct ′,3
R8794 T13513 T13500 prep by,generated
R8795 T13514 T13513 pobj PCR,by
R8796 T13515 T13500 advcl using,generated
R8797 T13516 T13517 det the,primer
R8798 T13517 T13515 dobj primer,using
R8799 T13518 T13517 amod forward,primer
R8800 T13519 T13520 nummod 5,ACCTTCTCCCGCATGTCCTTGCTCC
R8801 T13520 T13517 appos ACCTTCTCCCGCATGTCCTTGCTCC,primer
R8802 T13521 T13519 punct ′,5
R8803 T13522 T13520 punct -,ACCTTCTCCCGCATGTCCTTGCTCC
R8804 T13523 T13520 punct -,ACCTTCTCCCGCATGTCCTTGCTCC
R8805 T13524 T13520 nummod 3,ACCTTCTCCCGCATGTCCTTGCTCC
R8806 T13525 T13520 punct ′,ACCTTCTCCCGCATGTCCTTGCTCC
R8807 T13526 T13517 cc and,primer
R8808 T13527 T13528 det the,primer
R8809 T13528 T13517 conj primer,primer
R8810 T13529 T13528 amod reverse,primer
R8811 T13530 T13531 nummod 5,CTGCTGAGGCATGGTTACAGCTGG
R8812 T13531 T13528 appos CTGCTGAGGCATGGTTACAGCTGG,primer
R8813 T13532 T13530 punct ′,5
R8814 T13533 T13531 punct -,CTGCTGAGGCATGGTTACAGCTGG
R8815 T13534 T13531 punct -,CTGCTGAGGCATGGTTACAGCTGG
R8816 T13535 T13531 nummod 3,CTGCTGAGGCATGGTTACAGCTGG
R8817 T13536 T13531 punct ′,CTGCTGAGGCATGGTTACAGCTGG
R8818 T13537 T13528 punct ", ",primer
R8819 T13538 T13528 cc and,primer
R8820 T13539 T13540 amod genomic,DNA
R8821 T13540 T13528 conj DNA,primer
R8822 T13541 T13540 prep as,DNA
R8823 T13542 T13543 det a,template
R8824 T13543 T13541 pobj template,as
R8825 T13544 T13500 punct .,generated
R8826 T13546 T13547 det The,product
R8827 T13547 T13549 nsubjpass product,purified
R8828 T13548 T13547 compound PCR,product
R8829 T13550 T13549 auxpass was,purified
R8830 T13551 T13549 dep gel,purified
R8831 T13552 T13549 cc and,purified
R8832 T13553 T13549 conj ligated,purified
R8833 T13554 T13553 prep into,ligated
R8834 T13555 T13556 compound pCRII,TOPO
R8835 T13556 T13558 compound TOPO,vector
R8836 T13557 T13556 punct -,TOPO
R8837 T13558 T13554 pobj vector,into
R8838 T13559 T13558 compound TA,vector
R8839 T13560 T13549 punct .,purified
R8840 T13562 T13563 det The,domain
R8841 T13563 T13565 nsubjpass domain,generated
R8842 T13564 T13563 amod pre-LIM,domain
R8843 T13566 T13563 prep of,domain
R8844 T13567 T13566 pobj Ajuba,of
R8845 T13568 T13565 auxpass was,generated
R8846 T13569 T13565 advmod essentially,generated
R8847 T13570 T13571 mark as,described
R8848 T13571 T13565 advcl described,generated
R8849 T13572 T13573 punct [,9
R8850 T13573 T13571 parataxis 9,described
R8851 T13574 T13573 punct ],9
R8852 T13575 T13565 punct ", ",generated
R8853 T13576 T13565 cc but,generated
R8854 T13577 T13578 auxpass was,fused
R8855 T13578 T13565 conj fused,generated
R8856 T13579 T13578 prep to,fused
R8857 T13580 T13579 pobj GFP,to
R8858 T13581 T13578 prep by,fused
R8859 T13582 T13581 pcomp subcloning,by
R8860 T13583 T13582 prep from,subcloning
R8861 T13584 T13585 det the,vector
R8862 T13585 T13583 pobj vector,from
R8863 T13586 T13587 nmod pEGFP,N1
R8864 T13587 T13585 nmod N1,vector
R8865 T13588 T13587 punct -,N1
R8866 T13589 T13585 nummod 20,vector
R8867 T13590 T13591 punct (,Clontech
R8868 T13591 T13585 parataxis Clontech,vector
R8869 T13592 T13591 compound BD,Clontech
R8870 T13593 T13591 compound Biosciences,Clontech
R8871 T13594 T13591 punct ),Clontech
R8874 T13649 T13650 advmod In,situ
R8875 T13650 T13651 amod situ,hybridization
R8876 T13653 T13654 det The,vector
R8877 T13654 T13659 nsubjpass vector,used
R8878 T13655 T13656 compound pCRII,TOPO
R8879 T13656 T13654 compound TOPO,vector
R8880 T13657 T13656 punct -,TOPO
R8881 T13658 T13654 compound TA,vector
R8882 T13660 T13654 acl containing,vector
R8883 T13661 T13662 det a,region
R8884 T13662 T13660 dobj region,containing
R8885 T13663 T13662 prep of,region
R8886 T13664 T13665 det the,UTR
R8887 T13665 T13663 pobj UTR,of
R8888 T13666 T13665 nummod 3,UTR
R8889 T13667 T13666 punct ′,3
R8890 T13668 T13665 prep of,UTR
R8891 T13669 T13668 pobj Snail,of
R8892 T13670 T13659 auxpass was,used
R8893 T13671 T13659 prep as,used
R8894 T13672 T13673 det a,template
R8895 T13673 T13671 pobj template,as
R8896 T13674 T13675 aux to,generate
R8897 T13675 T13659 advcl generate,used
R8898 T13676 T13677 npadvmod digoxigenin,labeled
R8899 T13677 T13679 amod labeled,riboprobes
R8900 T13678 T13677 punct -,labeled
R8901 T13679 T13675 dobj riboprobes,generate
R8902 T13680 T13679 nmod sense,riboprobes
R8903 T13681 T13680 cc and,sense
R8904 T13682 T13680 conj antisense,sense
R8905 T13683 T13684 punct (,Roche
R8906 T13684 T13679 parataxis Roche,riboprobes
R8907 T13685 T13684 punct ),Roche
R8908 T13686 T13659 punct .,used
R8909 T13688 T13689 det The,probes
R8910 T13689 T13691 nsubjpass probes,obtained
R8911 T13690 T13689 amod respective,probes
R8912 T13692 T13691 auxpass were,obtained
R8913 T13693 T13691 prep by,obtained
R8914 T13694 T13695 nmod XhoI,digestions
R8915 T13695 T13693 pobj digestions,by
R8916 T13696 T13694 cc and,XhoI
R8917 T13697 T13694 conj BamH1,XhoI
R8918 T13698 T13691 punct .,obtained
R8919 T13700 T13701 advmod In,situ
R8920 T13701 T13702 amod situ,hybridizations
R8921 T13702 T13703 nsubjpass hybridizations,performed
R8922 T13704 T13703 auxpass were,performed
R8923 T13705 T13703 prep on,performed
R8924 T13706 T13707 nummod 10,μm
R8925 T13707 T13709 npadvmod μm,thick
R8926 T13708 T13707 punct -,μm
R8927 T13709 T13710 amod thick,sections
R8928 T13710 T13705 pobj sections,on
R8929 T13711 T13710 prep of,sections
R8930 T13712 T13713 compound E17.5,embryos
R8931 T13713 T13711 pobj embryos,of
R8932 T13714 T13713 compound mouse,embryos
R8933 T13715 T13703 punct .,performed
R8934 T13717 T13718 det The,sections
R8935 T13718 T13719 nsubjpass sections,fixed
R8936 T13720 T13719 auxpass were,fixed
R8937 T13721 T13719 prep with,fixed
R8938 T13722 T13723 nummod 4,%
R8939 T13723 T13724 compound %,PFA
R8940 T13724 T13721 pobj PFA,with
R8941 T13725 T13719 prep for,fixed
R8942 T13726 T13727 nummod 10,min
R8943 T13727 T13725 pobj min,for
R8944 T13728 T13719 prep at,fixed
R8945 T13729 T13730 compound room,temperature
R8946 T13730 T13728 pobj temperature,at
R8947 T13731 T13719 punct ", ",fixed
R8948 T13732 T13719 conj prehybridized,fixed
R8949 T13733 T13732 prep at,prehybridized
R8950 T13734 T13735 compound room,temperature
R8951 T13735 T13733 pobj temperature,at
R8952 T13736 T13732 prep for,prehybridized
R8953 T13737 T13738 nummod 4.5,h
R8954 T13738 T13736 pobj h,for
R8955 T13739 T13732 punct ", ",prehybridized
R8956 T13740 T13732 conj hybridized,prehybridized
R8957 T13741 T13740 prep with,hybridized
R8958 T13742 T13743 det the,probe
R8959 T13743 T13741 pobj probe,with
R8960 T13744 T13745 punct (,μg
R8961 T13745 T13743 parataxis μg,probe
R8962 T13746 T13745 nummod 2,μg
R8963 T13747 T13748 punct /,ml
R8964 T13748 T13745 prep ml,μg
R8965 T13749 T13745 punct ),μg
R8966 T13750 T13740 prep at,hybridized
R8967 T13751 T13752 nummod 55,°C
R8968 T13752 T13750 pobj °C,at
R8969 T13753 T13740 prep for,hybridized
R8970 T13754 T13755 quantmod 12,14
R8971 T13755 T13757 nummod 14,h
R8972 T13756 T13755 punct –,14
R8973 T13757 T13753 pobj h,for
R8974 T13758 T13740 punct ", ",hybridized
R8975 T13759 T13740 conj blocked,hybridized
R8976 T13760 T13759 prep with,blocked
R8977 T13761 T13762 nummod 10,%
R8978 T13762 T13763 compound %,NGS
R8979 T13763 T13760 pobj NGS,with
R8980 T13764 T13759 punct ", ",blocked
R8981 T13765 T13759 cc and,blocked
R8982 T13766 T13759 conj treated,blocked
R8983 T13767 T13766 prep with,treated
R8984 T13768 T13769 amod anti-dig,antibody
R8985 T13769 T13767 pobj antibody,with
R8986 T13770 T13771 compound Fab,AP
R8987 T13771 T13769 compound AP,antibody
R8988 T13772 T13771 punct -,AP
R8989 T13773 T13774 punct (,Roche
R8990 T13774 T13769 parataxis Roche,antibody
R8991 T13775 T13774 punct #,Roche
R8992 T13776 T13774 nummod 1093274,Roche
R8993 T13777 T13774 punct ),Roche
R8994 T13778 T13766 prep at,treated
R8995 T13779 T13780 det a,dilution
R8996 T13780 T13778 pobj dilution,at
R8997 T13781 T13782 quantmod 1,"2,500"
R8998 T13782 T13780 nummod "2,500",dilution
R8999 T13783 T13782 punct :,"2,500"
R9000 T13784 T13766 prep for,treated
R9001 T13785 T13786 nummod 3,h
R9002 T13786 T13784 pobj h,for
R9003 T13787 T13719 punct .,fixed
R9004 T13789 T13790 det The,sections
R9005 T13790 T13791 nsubjpass sections,incubated
R9006 T13792 T13791 auxpass were,incubated
R9007 T13793 T13791 prep with,incubated
R9008 T13794 T13793 pobj NBT,with
R9009 T13795 T13794 cc and,NBT
R9010 T13796 T13794 conj BCIP,NBT
R9011 T13797 T13798 mark until,detected
R9012 T13798 T13791 advcl detected,incubated
R9013 T13799 T13800 amod adequate,signal
R9014 T13800 T13798 nsubjpass signal,detected
R9015 T13801 T13798 auxpass was,detected
R9016 T13802 T13791 punct .,incubated
R9017 T13829 T13828 cc and,Immunofluorescence
R9018 T13830 T13828 conj immunohistochemistry,Immunofluorescence
R9019 T13832 T13833 compound Tissue,samples
R9020 T13833 T13834 nsubjpass samples,frozen
R9021 T13835 T13833 prep for,samples
R9022 T13836 T13835 pobj immunofluorescence,for
R9023 T13837 T13834 auxpass were,frozen
R9024 T13838 T13834 prep in,frozen
R9025 T13839 T13838 pobj OCT,in
R9026 T13840 T13834 cc and,frozen
R9027 T13841 T13834 conj sectioned,frozen
R9028 T13842 T13843 nummod 10,μm
R9029 T13843 T13844 npadvmod μm,thick
R9030 T13844 T13841 advcl thick,sectioned
R9031 T13845 T13841 prep on,sectioned
R9032 T13846 T13847 det a,cryostat
R9033 T13847 T13845 pobj cryostat,on
R9034 T13848 T13834 punct .,frozen
R9035 T13850 T13851 nsubjpass Sections,fixed
R9036 T13852 T13851 auxpass were,fixed
R9037 T13853 T13851 prep in,fixed
R9038 T13854 T13855 nummod 4,%
R9039 T13855 T13856 compound %,paraformaldehyde
R9040 T13856 T13853 pobj paraformaldehyde,in
R9041 T13857 T13851 prep for,fixed
R9042 T13858 T13859 nummod 10,min
R9043 T13859 T13857 pobj min,for
R9044 T13860 T13851 prep at,fixed
R9045 T13861 T13862 compound room,temperature
R9046 T13862 T13860 pobj temperature,at
R9047 T13863 T13851 punct ", ",fixed
R9048 T13864 T13851 conj blocked,fixed
R9049 T13865 T13864 punct ", ",blocked
R9050 T13866 T13864 cc and,blocked
R9051 T13867 T13864 conj stained,blocked
R9052 T13868 T13867 prep with,stained
R9053 T13869 T13868 pobj antibodies,with
R9054 T13870 T13851 punct .,fixed
R9055 T13872 T13873 compound Tissue,samples
R9056 T13873 T13874 nsubjpass samples,fixed
R9057 T13875 T13873 prep for,samples
R9058 T13876 T13875 pobj immunohistochemistry,for
R9059 T13877 T13874 auxpass were,fixed
R9060 T13878 T13874 prep in,fixed
R9061 T13879 T13880 nummod 4,%
R9062 T13880 T13881 compound %,paraformaldehyde
R9063 T13881 T13878 pobj paraformaldehyde,in
R9064 T13882 T13874 punct ", ",fixed
R9065 T13883 T13874 conj dehydrated,fixed
R9066 T13884 T13883 punct ", ",dehydrated
R9067 T13885 T13883 cc and,dehydrated
R9068 T13886 T13883 conj embedded,dehydrated
R9069 T13887 T13886 prep in,embedded
R9070 T13888 T13887 pobj paraffin,in
R9071 T13889 T13874 punct .,fixed
R9072 T13891 T13892 nsubjpass Samples,sectioned
R9073 T13893 T13892 auxpass were,sectioned
R9074 T13894 T13892 prep on,sectioned
R9075 T13895 T13896 det a,microtome
R9076 T13896 T13894 pobj microtome,on
R9077 T13897 T13898 punct (,thick
R9078 T13898 T13892 parataxis thick,sectioned
R9079 T13899 T13900 nummod 10,μm
R9080 T13900 T13898 npadvmod μm,thick
R9081 T13901 T13898 punct ),thick
R9082 T13902 T13892 cc and,sectioned
R9083 T13903 T13892 conj rehydrated,sectioned
R9084 T13904 T13903 prep prior,rehydrated
R9085 T13905 T13904 pcomp to,prior
R9086 T13906 T13904 pcomp staining,prior
R9087 T13907 T13906 prep with,staining
R9088 T13908 T13907 pobj antibody,with
R9089 T13909 T13892 punct .,sectioned
R9090 T13911 T13912 nsubjpass Samples,unmasked
R9091 T13913 T13911 acl stained,Samples
R9092 T13914 T13913 prep with,stained
R9093 T13915 T13914 pobj Snail,with
R9094 T13916 T13915 punct ", ",Snail
R9095 T13917 T13915 conj pMAPK,Snail
R9096 T13918 T13917 punct ", ",pMAPK
R9097 T13919 T13917 conj pSmad2,pMAPK
R9098 T13920 T13919 punct ", ",pSmad2
R9099 T13921 T13919 cc and,pSmad2
R9100 T13922 T13923 compound cyclin,D
R9101 T13923 T13919 conj D,pSmad2
R9102 T13924 T13912 auxpass were,unmasked
R9103 T13925 T13912 dep antigen,unmasked
R9104 T13926 T13912 prep with,unmasked
R9105 T13927 T13928 nummod 10,mM
R9106 T13928 T13929 compound mM,citrate
R9107 T13929 T13926 pobj citrate,with
R9108 T13930 T13929 compound sodium,citrate
R9109 T13931 T13929 punct (,citrate
R9110 T13932 T13929 npadvmod pH,citrate
R9111 T13933 T13932 nummod 6,pH
R9112 T13934 T13929 punct ),citrate
R9113 T13935 T13912 prep in,unmasked
R9114 T13936 T13937 det an,Retriever
R9115 T13937 T13935 pobj Retriever,in
R9116 T13938 T13937 compound Antigen,Retriever
R9117 T13939 T13937 nummod 2100,Retriever
R9118 T13940 T13941 punct (,Laboratories
R9119 T13941 T13937 parataxis Laboratories,Retriever
R9120 T13942 T13941 compound Pickcell,Laboratories
R9121 T13943 T13941 punct ", ",Laboratories
R9122 T13944 T13941 npadvmod Leiden,Laboratories
R9123 T13945 T13941 punct ", ",Laboratories
R9124 T13946 T13941 npadvmod Netherlands,Laboratories
R9125 T13947 T13941 punct ),Laboratories
R9126 T13948 T13912 punct .,unmasked
R9127 T13950 T13951 det The,kit
R9128 T13951 T13954 nsubjpass kit,used
R9129 T13952 T13953 compound DAB,substrate
R9130 T13953 T13951 compound substrate,kit
R9131 T13955 T13956 punct (,Labs
R9132 T13956 T13951 parataxis Labs,kit
R9133 T13957 T13956 compound Vector,Labs
R9134 T13958 T13956 punct ),Labs
R9135 T13959 T13954 auxpass was,used
R9136 T13960 T13954 prep according,used
R9137 T13961 T13960 prep to,according
R9138 T13962 T13963 poss manufacturer,instructions
R9139 T13963 T13961 pobj instructions,to
R9140 T13964 T13962 case 's,manufacturer
R9141 T13965 T13966 aux to,develop
R9142 T13966 T13954 advcl develop,used
R9143 T13967 T13968 det the,signal
R9144 T13968 T13966 dobj signal,develop
R9145 T13969 T13954 punct .,used
R9146 T14008 T14009 compound RT,PCR
R9147 T14010 T14009 punct -,PCR
R9148 T14012 T14013 nsubjpass RNA,extracted
R9149 T14014 T14013 auxpass was,extracted
R9150 T14015 T14013 prep from,extracted
R9151 T14016 T14015 pobj keratinocytes,from
R9152 T14017 T14016 cc or,keratinocytes
R9153 T14018 T14019 compound skin,tissue
R9154 T14019 T14016 conj tissue,keratinocytes
R9155 T14020 T14013 prep with,extracted
R9156 T14021 T14020 pobj Trizol,with
R9157 T14022 T14023 punct (,Invitrogen
R9158 T14023 T14021 parataxis Invitrogen,Trizol
R9159 T14024 T14023 punct ),Invitrogen
R9160 T14025 T14013 prep according,extracted
R9161 T14026 T14025 prep to,according
R9162 T14027 T14028 det the,manufacturer
R9163 T14028 T14029 poss manufacturer,protocol
R9164 T14029 T14026 pobj protocol,to
R9165 T14030 T14028 case 's,manufacturer
R9166 T14031 T14013 punct .,extracted
R9167 T14033 T14034 nsubjpass cDNA,generated
R9168 T14035 T14034 auxpass was,generated
R9169 T14036 T14034 advcl using,generated
R9170 T14037 T14038 compound oligo,dT
R9171 T14038 T14040 compound dT,primers
R9172 T14039 T14038 punct -,dT
R9173 T14040 T14036 dobj primers,using
R9174 T14041 T14040 cc and,primers
R9175 T14042 T14043 det the,kit
R9176 T14043 T14040 conj kit,primers
R9177 T14044 T14043 nmod Superscript,kit
R9178 T14045 T14044 nummod II,Superscript
R9179 T14046 T14047 punct (,Invitrogen
R9180 T14047 T14043 parataxis Invitrogen,kit
R9181 T14048 T14047 punct ),Invitrogen
R9182 T14049 T14034 punct .,generated
R9183 T14051 T14052 det The,primers
R9184 T14052 T14053 nsubj primers,were
R9185 T14054 T14052 acl used,primers
R9186 T14055 T14054 prep for,used
R9187 T14056 T14055 pobj PCR,for
R9188 T14057 T14058 compound Snail,forward
R9189 T14058 T14053 attr forward,were
R9190 T14059 T14058 punct : ,forward
R9191 T14060 T14061 nummod 5,CAGCTGGCCAGGCTCTCGGT
R9192 T14061 T14058 appos CAGCTGGCCAGGCTCTCGGT,forward
R9193 T14062 T14060 punct ′,5
R9194 T14063 T14061 punct -,CAGCTGGCCAGGCTCTCGGT
R9195 T14064 T14061 punct -,CAGCTGGCCAGGCTCTCGGT
R9196 T14065 T14061 nummod 3,CAGCTGGCCAGGCTCTCGGT
R9197 T14066 T14061 punct ′,CAGCTGGCCAGGCTCTCGGT
R9198 T14067 T14058 punct ;,forward
R9199 T14068 T14069 compound Snail,reverse
R9200 T14069 T14058 conj reverse,forward
R9201 T14070 T14069 punct : ,reverse
R9202 T14071 T14072 nummod 5,GCGAGGGCCTCCGGAGCA
R9203 T14072 T14069 appos GCGAGGGCCTCCGGAGCA,reverse
R9204 T14073 T14071 punct ′,5
R9205 T14074 T14072 punct -,GCGAGGGCCTCCGGAGCA
R9206 T14075 T14072 punct -,GCGAGGGCCTCCGGAGCA
R9207 T14076 T14072 nummod 3,GCGAGGGCCTCCGGAGCA
R9208 T14077 T14072 punct ′,GCGAGGGCCTCCGGAGCA
R9209 T14078 T14069 punct ;,reverse
R9210 T14079 T14080 compound GAPDH,forward
R9211 T14080 T14069 conj forward,reverse
R9212 T14081 T14082 nummod 5,CGTAGACAAAATGGTGAAGGTCGG
R9213 T14082 T14080 appos CGTAGACAAAATGGTGAAGGTCGG,forward
R9214 T14083 T14081 punct ′,5
R9215 T14084 T14082 punct -,CGTAGACAAAATGGTGAAGGTCGG
R9216 T14085 T14082 punct -,CGTAGACAAAATGGTGAAGGTCGG
R9217 T14086 T14082 nummod 3,CGTAGACAAAATGGTGAAGGTCGG
R9218 T14087 T14082 punct ′,CGTAGACAAAATGGTGAAGGTCGG
R9219 T14088 T14080 punct ;,forward
R9220 T14089 T14080 cc and,forward
R9221 T14090 T14091 compound GAPDH,reverse
R9222 T14091 T14080 conj reverse,forward
R9223 T14092 T14091 punct : ,reverse
R9224 T14093 T14094 nummod 5,AAGCAGTTGGTGGTGCAGGATG
R9225 T14094 T14091 appos AAGCAGTTGGTGGTGCAGGATG,reverse
R9226 T14095 T14093 punct ′,5
R9227 T14096 T14094 punct -,AAGCAGTTGGTGGTGCAGGATG
R9228 T14097 T14094 punct -,AAGCAGTTGGTGGTGCAGGATG
R9229 T14098 T14094 nummod 3,AAGCAGTTGGTGGTGCAGGATG
R9230 T14099 T14094 punct ′,AAGCAGTTGGTGGTGCAGGATG
R9231 T14100 T14053 punct .,were
R2 T149 T147 compound Signaling,Pathway
R3 T150 T147 acl Involving,Pathway
R4 T151 T152 compound TGF,β2
R7 T154 T152 cc and,β2
R8 T155 T152 conj Snail,β2
R9 T156 T147 prep in,Pathway
R10 T157 T158 compound Hair,Follicle
R11 T158 T159 compound Follicle,Morphogenesis
R12 T159 T156 pobj Morphogenesis,in
R13 T162 T163 prep In,exchange
R14 T164 T165 det a,theme
R17 T167 T165 prep of,theme
R18 T168 T167 pobj organogenesis,of
R19 T169 T163 punct ", ",exchange
R20 T170 T171 amod certain,cells
R21 T171 T163 nsubj cells,exchange
R22 T172 T171 prep within,cells
R23 T173 T174 det a,sheet
R27 T177 T163 dobj signals,exchange
R28 T178 T163 prep with,exchange
R29 T179 T180 poss their,neighbors
R30 T180 T178 pobj neighbors,with
R31 T181 T163 cc and,exchange
R32 T182 T163 conj develop,exchange
R33 T183 T182 prep into,develop
R34 T184 T185 det a,structure
R37 T187 T163 punct .,exchange
R38 T189 T190 advcl Using,employed
R39 T191 T192 compound hair,bud
R40 T192 T193 compound bud,morphogenesis
R41 T193 T189 dobj morphogenesis,Using
R42 T194 T189 prep as,Using
R43 T195 T196 det a,paradigm
R44 T196 T194 pobj paradigm,as
R45 T197 T190 punct ", ",employed
R46 T198 T190 nsubj we,employed
R47 T199 T200 compound mutant,mouse
R48 T200 T201 compound mouse,models
R49 T201 T190 dobj models,employed
R50 T202 T201 cc and,models
R51 T203 T204 amod cultured,keratinocytes
R52 T204 T201 conj keratinocytes,models
R53 T205 T206 aux to,dissect
R54 T206 T190 advcl dissect,employed
R55 T207 T208 det the,contributions
R56 T208 T206 dobj contributions,dissect
R57 T209 T208 prep of,contributions
R58 T210 T211 amod multiple,cues
R61 T213 T208 prep in,contributions
R62 T214 T213 pcomp orchestrating,in
R63 T215 T216 compound adhesion,dynamics
R64 T216 T214 dobj dynamics,orchestrating
R65 T217 T216 cc and,dynamics
R66 T218 T216 conj proliferation,dynamics
R67 T219 T220 aux to,shape
R68 T220 T214 advcl shape,orchestrating
R69 T221 T222 det the,cluster
R70 T222 T220 dobj cluster,shape
R71 T223 T222 prep of,cluster
R72 T224 T223 pobj cells,of
R73 T225 T222 acl involved,cluster
R74 T226 T190 punct .,employed
R75 T228 T229 nsubj We,found
R76 T230 T231 mark that,is
R80 T234 T236 compound β2,signaling
R83 T237 T231 acomp necessary,is
R84 T238 T239 aux to,induce
R87 T241 T242 det the,Snail
R91 T245 T239 cc and,induce
R92 T246 T239 conj activate,induce
R93 T247 T248 det the,pathway
R105 T259 T246 prep in,activate
R106 T260 T261 det the,bud
R107 T261 T259 pobj bud,in
R108 T262 T229 punct .,found
R109 T264 T265 prep In,leads
R110 T266 T267 det the,epidermis
R111 T267 T264 pobj epidermis,In
R112 T268 T265 punct ", ",leads
R113 T269 T270 compound Snail,misexpression
R114 T270 T265 nsubj misexpression,leads
R115 T271 T265 prep to,leads
R116 T272 T271 pobj hyperproliferation,to
R117 T273 T272 cc and,hyperproliferation
R118 T274 T275 det a,reduction
R119 T275 T272 conj reduction,hyperproliferation
R120 T276 T275 prep in,reduction
R121 T277 T278 amod intercellular,adhesion
R122 T278 T276 pobj adhesion,in
R123 T279 T265 punct .,leads
R124 T281 T282 advmod When,regulated
R127 T284 T282 nsubjpass cadherin,regulated
R133 T291 T290 punct ", ",released
R134 T292 T293 amod associated,proteins
R137 T295 T293 prep with,proteins
R138 T296 T297 amod dual,functions
R139 T297 T295 pobj functions,with
R140 T298 T297 prep in,functions
R141 T299 T298 pobj signaling,in
R142 T300 T290 auxpass are,released
R143 T301 T290 prep from,released
R144 T302 T303 compound cell,cell
R147 T305 T301 pobj contacts,from
R148 T306 T290 punct ", ",released
R149 T307 T308 det a,process
R150 T308 T290 npadvmod process,released
R151 T309 T310 dep which,demonstrate
R154 T312 T310 advcl leads,demonstrate
R155 T313 T312 prep to,leads
R156 T314 T315 compound Ras,MAPK
R159 T317 T313 pobj activation,to
R160 T318 T290 punct .,released
R161 T320 T321 det These,studies
R162 T321 T322 nsubj studies,provide
R163 T323 T322 dobj insights,provide
R164 T324 T323 prep into,insights
R165 T325 T326 advmod how,stimulated
R173 T333 T334 aux to,undergo
R174 T334 T326 advcl undergo,stimulated
R175 T335 T336 amod transcriptional,changes
R176 T336 T334 dobj changes,undergo
R177 T337 T338 dep that,result
R178 T338 T336 relcl result,changes
R179 T339 T338 prep in,result
R180 T340 T339 pobj proliferation,in
R181 T341 T340 punct ", ",proliferation
R182 T342 T343 amod junctional,remodeling
R183 T343 T340 conj remodeling,proliferation
R184 T344 T343 punct ", ",remodeling
R185 T345 T343 cc and,remodeling
R186 T346 T347 compound bud,formation
R187 T347 T343 conj formation,remodeling
R188 T348 T322 punct .,provide
R189 T350 T351 det This,pathway
R193 T355 T354 advmod further,weaves
R194 T356 T354 prt together,weaves
R195 T357 T358 det the,web
R196 T358 T354 dobj web,weaves
R197 T359 T358 prep of,web
R198 T360 T361 amod different,morphogens
R199 T361 T359 pobj morphogens,of
R200 T362 T361 cc and,morphogens
R201 T363 T364 amod downstream,events
R204 T366 T367 dep that,guide
R205 T367 T358 relcl guide,web
R206 T368 T369 compound hair,bud
R207 T369 T370 compound bud,formation
R208 T370 T367 dobj formation,guide
R209 T371 T367 prep within,guide
R210 T372 T373 det the,skin
R213 T375 T354 punct .,weaves
R1368 T2241 T2240 nmod follicle,growth
R1369 T2242 T2240 amod down,growth
R3692 T6120 T6119 pobj MAPK,of
R3693 T6121 T6122 punct [,9
R3879 T6317 T6301 auxpass be,expected
R4072 T6522 T6523 det the,pathway
R4081 T6530 T6533 advcl provides,known
R5525 T8845 T8844 punct -,mediated
R5530 T8850 T8851 compound E,cadherin
R5729 T9008 T8986 punct .,suggest
R5730 T9010 T9011 advmod Moreover,appears
R5731 T9012 T9011 punct ", ",appears
R5732 T9013 T9011 prep through,appears
R5733 T9014 T9013 pobj activation,through
R5734 T9015 T9014 prep of,activation
R5735 T9016 T9017 compound Snail,expression
R5736 T9017 T9015 pobj expression,of
R5737 T9018 T9017 compound gene,expression
R5738 T9019 T9011 punct ", ",appears
R5739 T9020 T9021 compound TGF,β2
R5740 T9021 T9011 nsubj β2,appears
R5741 T9022 T9021 punct -,β2
R5742 T9023 T9024 aux to,work
R5743 T9024 T9011 xcomp work,appears
R5744 T9025 T9024 prep in,work
R5745 T9026 T9025 pobj tandem,in
R5746 T9027 T9026 prep with,tandem
R5747 T9028 T9029 det these,morphogens
R5748 T9029 T9027 pobj morphogens,with
R5749 T9030 T9029 amod other,morphogens
R5750 T9031 T9032 aux to,regulate
R5751 T9032 T9024 advcl regulate,work
R5752 T9033 T9032 advmod down,regulate
R5753 T9034 T9032 punct -,regulate
R5754 T9035 T9036 compound E,cadherin
R5755 T9036 T9038 compound cadherin,levels
R5756 T9037 T9036 punct -,cadherin
R5757 T9038 T9032 dobj levels,regulate
R5758 T9039 T9024 punct ", ",work
R5759 T9040 T9041 dep which,contributes
R5760 T9041 T9024 advcl contributes,work
R5761 T9042 T9041 prep to,contributes
R5762 T9043 T9044 det the,activation
R5763 T9044 T9042 pobj activation,to
R5764 T9045 T9044 prep of,activation
R5765 T9046 T9047 amod proliferative,circuitries
R5766 T9047 T9045 pobj circuitries,of
R5767 T9048 T9011 punct .,appears