Id |
Subject |
Object |
Predicate |
Lexical cue |
T18468 |
0-2 |
FW |
denotes |
In |
T18469 |
3-8 |
FW |
denotes |
vitro |
T18470 |
20-25 |
NN |
denotes |
assay |
T18471 |
9-19 |
NN |
denotes |
co-culture |
T18472 |
25-26 |
. |
denotes |
. |
T18473 |
26-116 |
sentence |
denotes |
DRG explants were derived from Swiss Webster, Bax null, and p75 null mouse embryos (E13). |
T18474 |
27-30 |
NN |
denotes |
DRG |
T18475 |
31-39 |
NNS |
denotes |
explants |
T18476 |
45-52 |
VBN |
denotes |
derived |
T18477 |
40-44 |
VBD |
denotes |
were |
T18478 |
53-57 |
IN |
denotes |
from |
T18479 |
58-63 |
NNP |
denotes |
Swiss |
T18480 |
64-71 |
NNP |
denotes |
Webster |
T18481 |
102-109 |
NNS |
denotes |
embryos |
T18482 |
71-73 |
, |
denotes |
, |
T18483 |
73-76 |
NNP |
denotes |
Bax |
T18484 |
77-81 |
JJ |
denotes |
null |
T18485 |
81-83 |
, |
denotes |
, |
T18486 |
83-86 |
CC |
denotes |
and |
T18487 |
87-90 |
NN |
denotes |
p75 |
T18488 |
91-95 |
JJ |
denotes |
null |
T18489 |
96-101 |
NN |
denotes |
mouse |
T18490 |
110-111 |
-LRB- |
denotes |
( |
T18491 |
111-114 |
NN |
denotes |
E13 |
T18492 |
114-115 |
-RRB- |
denotes |
) |
T18493 |
115-116 |
. |
denotes |
. |
T18494 |
116-217 |
sentence |
denotes |
For collagen matrix assays, DRGs were dissected out under a stereomicroscope using tungsten needles. |
T18495 |
117-120 |
IN |
denotes |
For |
T18496 |
155-164 |
VBN |
denotes |
dissected |
T18497 |
121-129 |
NN |
denotes |
collagen |
T18498 |
130-136 |
NN |
denotes |
matrix |
T18499 |
137-143 |
NNS |
denotes |
assays |
T18500 |
143-145 |
, |
denotes |
, |
T18501 |
145-149 |
NNS |
denotes |
DRGs |
T18502 |
150-154 |
VBD |
denotes |
were |
T18503 |
165-168 |
RP |
denotes |
out |
T18504 |
169-174 |
IN |
denotes |
under |
T18505 |
175-176 |
DT |
denotes |
a |
T18506 |
177-193 |
NN |
denotes |
stereomicroscope |
T18507 |
194-199 |
VBG |
denotes |
using |
T18508 |
200-208 |
NN |
denotes |
tungsten |
T18509 |
209-216 |
NNS |
denotes |
needles |
T18510 |
216-217 |
. |
denotes |
. |
T18511 |
217-403 |
sentence |
denotes |
Collagen matrix was prepared with 430 μl of collagen (3 mg/ml dissolved in 0.1M acetic acid, Sigma), 50 μl of 10X DMEM medium, and 2.5 μl of 0.8 M NaCa2, and the pH was adjusted to 7.5. |
T18512 |
218-226 |
NN |
denotes |
Collagen |
T18513 |
227-233 |
NN |
denotes |
matrix |
T18514 |
238-246 |
VBN |
denotes |
prepared |
T18515 |
234-237 |
VBD |
denotes |
was |
T18516 |
247-251 |
IN |
denotes |
with |
T18517 |
252-255 |
CD |
denotes |
430 |
T18518 |
256-258 |
NN |
denotes |
μl |
T18519 |
259-261 |
IN |
denotes |
of |
T18520 |
262-270 |
NN |
denotes |
collagen |
T18521 |
271-272 |
-LRB- |
denotes |
( |
T18522 |
311-316 |
NNP |
denotes |
Sigma |
T18523 |
272-273 |
CD |
denotes |
3 |
T18524 |
274-276 |
NN |
denotes |
mg |
T18525 |
276-277 |
SYM |
denotes |
/ |
T18526 |
277-279 |
NN |
denotes |
ml |
T18527 |
280-289 |
VBN |
denotes |
dissolved |
T18528 |
290-292 |
IN |
denotes |
in |
T18529 |
293-296 |
CD |
denotes |
0.1 |
T18530 |
296-297 |
NN |
denotes |
M |
T18531 |
305-309 |
NN |
denotes |
acid |
T18532 |
298-304 |
JJ |
denotes |
acetic |
T18533 |
309-311 |
, |
denotes |
, |
T18534 |
316-317 |
-RRB- |
denotes |
) |
T18535 |
317-319 |
, |
denotes |
, |
T18536 |
319-321 |
CD |
denotes |
50 |
T18537 |
322-324 |
NN |
denotes |
μl |
T18538 |
325-327 |
IN |
denotes |
of |
T18539 |
328-330 |
CD |
denotes |
10 |
T18540 |
332-336 |
NN |
denotes |
DMEM |
T18541 |
330-331 |
NN |
denotes |
X |
T18542 |
337-343 |
NN |
denotes |
medium |
T18543 |
343-345 |
, |
denotes |
, |
T18544 |
345-348 |
CC |
denotes |
and |
T18545 |
349-352 |
CD |
denotes |
2.5 |
T18546 |
353-355 |
NN |
denotes |
μl |
T18547 |
356-358 |
IN |
denotes |
of |
T18548 |
359-362 |
CD |
denotes |
0.8 |
T18549 |
363-364 |
NN |
denotes |
M |
T18550 |
365-370 |
NN |
denotes |
NaCa2 |
T18551 |
370-372 |
, |
denotes |
, |
T18552 |
372-375 |
CC |
denotes |
and |
T18553 |
376-379 |
DT |
denotes |
the |
T18554 |
380-382 |
NN |
denotes |
pH |
T18555 |
387-395 |
VBN |
denotes |
adjusted |
T18556 |
383-386 |
VBD |
denotes |
was |
T18557 |
396-398 |
IN |
denotes |
to |
T18558 |
399-402 |
CD |
denotes |
7.5 |
T18559 |
402-403 |
. |
denotes |
. |
T18560 |
403-506 |
sentence |
denotes |
Individual ganglion explants were placed in 24-well plates and covered with freshly prepared collagen. |
T18561 |
404-414 |
JJ |
denotes |
Individual |
T18562 |
424-432 |
NNS |
denotes |
explants |
T18563 |
415-423 |
NN |
denotes |
ganglion |
T18564 |
438-444 |
VBN |
denotes |
placed |
T18565 |
433-437 |
VBD |
denotes |
were |
T18566 |
445-447 |
IN |
denotes |
in |
T18567 |
448-450 |
CD |
denotes |
24 |
T18568 |
451-455 |
NN |
denotes |
well |
T18569 |
450-451 |
HYPH |
denotes |
- |
T18570 |
456-462 |
NNS |
denotes |
plates |
T18571 |
463-466 |
CC |
denotes |
and |
T18572 |
467-474 |
VBN |
denotes |
covered |
T18573 |
475-479 |
IN |
denotes |
with |
T18574 |
480-487 |
RB |
denotes |
freshly |
T18575 |
488-496 |
VBN |
denotes |
prepared |
T18576 |
497-505 |
NN |
denotes |
collagen |
T18577 |
505-506 |
. |
denotes |
. |
T18578 |
506-568 |
sentence |
denotes |
Sepharose beads with an average diameter of 150 μm were used. |
T18579 |
507-516 |
NN |
denotes |
Sepharose |
T18580 |
517-522 |
NNS |
denotes |
beads |
T18581 |
563-567 |
VBN |
denotes |
used |
T18582 |
523-527 |
IN |
denotes |
with |
T18583 |
528-530 |
DT |
denotes |
an |
T18584 |
539-547 |
NN |
denotes |
diameter |
T18585 |
531-538 |
JJ |
denotes |
average |
T18586 |
548-550 |
IN |
denotes |
of |
T18587 |
551-554 |
CD |
denotes |
150 |
T18588 |
555-557 |
NN |
denotes |
μm |
T18589 |
558-562 |
VBD |
denotes |
were |
T18590 |
567-568 |
. |
denotes |
. |
T18591 |
568-735 |
sentence |
denotes |
Beads were washed twice with PBS, air dried, and loaded with 10, 20, 50, or 100 ng/μl NT-3 (Collaborative Research, Chemicon) at 4 °C overnight with constant shaking. |
T18592 |
569-574 |
NNS |
denotes |
Beads |
T18593 |
580-586 |
VBN |
denotes |
washed |
T18594 |
575-579 |
VBD |
denotes |
were |
T18595 |
587-592 |
RB |
denotes |
twice |
T18596 |
593-597 |
IN |
denotes |
with |
T18597 |
598-601 |
NN |
denotes |
PBS |
T18598 |
601-603 |
, |
denotes |
, |
T18599 |
603-606 |
NN |
denotes |
air |
T18600 |
607-612 |
VBN |
denotes |
dried |
T18601 |
612-614 |
, |
denotes |
, |
T18602 |
614-617 |
CC |
denotes |
and |
T18603 |
618-624 |
VBN |
denotes |
loaded |
T18604 |
625-629 |
IN |
denotes |
with |
T18605 |
630-632 |
CD |
denotes |
10 |
T18606 |
649-651 |
NN |
denotes |
ng |
T18607 |
632-634 |
, |
denotes |
, |
T18608 |
634-636 |
CD |
denotes |
20 |
T18609 |
636-638 |
, |
denotes |
, |
T18610 |
638-640 |
CD |
denotes |
50 |
T18611 |
640-642 |
, |
denotes |
, |
T18612 |
642-644 |
CC |
denotes |
or |
T18613 |
645-648 |
CD |
denotes |
100 |
T18614 |
655-657 |
NN |
denotes |
NT |
T18615 |
651-652 |
SYM |
denotes |
/ |
T18616 |
652-654 |
NN |
denotes |
μl |
T18617 |
657-658 |
HYPH |
denotes |
- |
T18618 |
658-659 |
CD |
denotes |
3 |
T18619 |
660-661 |
-LRB- |
denotes |
( |
T18620 |
675-683 |
NN |
denotes |
Research |
T18621 |
661-674 |
JJ |
denotes |
Collaborative |
T18622 |
683-685 |
, |
denotes |
, |
T18623 |
685-693 |
NNP |
denotes |
Chemicon |
T18624 |
693-694 |
-RRB- |
denotes |
) |
T18625 |
695-697 |
IN |
denotes |
at |
T18626 |
698-699 |
CD |
denotes |
4 |
T18627 |
700-702 |
NN |
denotes |
°C |
T18628 |
703-712 |
RB |
denotes |
overnight |
T18629 |
713-717 |
IN |
denotes |
with |
T18630 |
718-726 |
JJ |
denotes |
constant |
T18631 |
727-734 |
NN |
denotes |
shaking |
T18632 |
734-735 |
. |
denotes |
. |
T18633 |
735-807 |
sentence |
denotes |
For negative control, beads were loaded with BSA (10–100 ng/μl) or PBS. |
T18634 |
736-739 |
IN |
denotes |
For |
T18635 |
769-775 |
VBN |
denotes |
loaded |
T18636 |
740-748 |
JJ |
denotes |
negative |
T18637 |
749-756 |
NN |
denotes |
control |
T18638 |
756-758 |
, |
denotes |
, |
T18639 |
758-763 |
NNS |
denotes |
beads |
T18640 |
764-768 |
VBD |
denotes |
were |
T18641 |
776-780 |
IN |
denotes |
with |
T18642 |
781-784 |
NN |
denotes |
BSA |
T18643 |
785-786 |
-LRB- |
denotes |
( |
T18644 |
793-795 |
NN |
denotes |
ng |
T18645 |
786-788 |
CD |
denotes |
10 |
T18646 |
789-792 |
CD |
denotes |
100 |
T18647 |
788-789 |
HYPH |
denotes |
– |
T18648 |
795-796 |
SYM |
denotes |
/ |
T18649 |
796-798 |
NN |
denotes |
μl |
T18650 |
798-799 |
-RRB- |
denotes |
) |
T18651 |
800-802 |
CC |
denotes |
or |
T18652 |
803-806 |
NN |
denotes |
PBS |
T18653 |
806-807 |
. |
denotes |
. |
T18654 |
807-950 |
sentence |
denotes |
Either a single neurotrophin-loaded bead or a single BSA (or PBS)–loaded bead was implanted about 200–1,200 μm away from the ganglion explant. |
T18655 |
808-814 |
CC |
denotes |
Either |
T18656 |
844-848 |
NN |
denotes |
bead |
T18657 |
815-816 |
DT |
denotes |
a |
T18658 |
817-823 |
JJ |
denotes |
single |
T18659 |
824-836 |
NN |
denotes |
neurotrophin |
T18660 |
837-843 |
VBN |
denotes |
loaded |
T18661 |
836-837 |
HYPH |
denotes |
- |
T18662 |
890-899 |
VBN |
denotes |
implanted |
T18663 |
849-851 |
CC |
denotes |
or |
T18664 |
852-853 |
DT |
denotes |
a |
T18665 |
881-885 |
NN |
denotes |
bead |
T18666 |
854-860 |
JJ |
denotes |
single |
T18667 |
861-864 |
NN |
denotes |
BSA |
T18668 |
874-880 |
VBN |
denotes |
loaded |
T18669 |
865-866 |
-LRB- |
denotes |
( |
T18670 |
866-868 |
CC |
denotes |
or |
T18671 |
869-872 |
NN |
denotes |
PBS |
T18672 |
872-873 |
-RRB- |
denotes |
) |
T18673 |
873-874 |
HYPH |
denotes |
– |
T18674 |
886-889 |
VBD |
denotes |
was |
T18675 |
900-905 |
IN |
denotes |
about |
T18676 |
910-915 |
CD |
denotes |
1,200 |
T18677 |
906-909 |
CD |
denotes |
200 |
T18678 |
909-910 |
HYPH |
denotes |
– |
T18679 |
916-918 |
NN |
denotes |
μm |
T18680 |
919-923 |
RB |
denotes |
away |
T18681 |
924-928 |
IN |
denotes |
from |
T18682 |
929-932 |
DT |
denotes |
the |
T18683 |
942-949 |
NN |
denotes |
explant |
T18684 |
933-941 |
NN |
denotes |
ganglion |
T18685 |
949-950 |
. |
denotes |
. |
T18686 |
950-1040 |
sentence |
denotes |
Collagen-embedded cultures were then placed at 33 °C for 15 min for the matrix to harden. |
T18687 |
951-959 |
NN |
denotes |
Collagen |
T18688 |
960-968 |
VBN |
denotes |
embedded |
T18689 |
959-960 |
HYPH |
denotes |
- |
T18690 |
969-977 |
NNS |
denotes |
cultures |
T18691 |
988-994 |
VBN |
denotes |
placed |
T18692 |
978-982 |
VBD |
denotes |
were |
T18693 |
983-987 |
RB |
denotes |
then |
T18694 |
995-997 |
IN |
denotes |
at |
T18695 |
998-1000 |
CD |
denotes |
33 |
T18696 |
1001-1003 |
NN |
denotes |
°C |
T18697 |
1004-1007 |
IN |
denotes |
for |
T18698 |
1008-1010 |
CD |
denotes |
15 |
T18699 |
1011-1014 |
NN |
denotes |
min |
T18700 |
1015-1018 |
IN |
denotes |
for |
T18701 |
1033-1039 |
VB |
denotes |
harden |
T18702 |
1019-1022 |
DT |
denotes |
the |
T18703 |
1023-1029 |
NN |
denotes |
matrix |
T18704 |
1030-1032 |
TO |
denotes |
to |
T18705 |
1039-1040 |
. |
denotes |
. |
T18706 |
1040-1095 |
sentence |
denotes |
Serum-free culture medium was then added to each well. |
T18707 |
1041-1046 |
NN |
denotes |
Serum |
T18708 |
1047-1051 |
JJ |
denotes |
free |
T18709 |
1046-1047 |
HYPH |
denotes |
- |
T18710 |
1060-1066 |
NN |
denotes |
medium |
T18711 |
1052-1059 |
NN |
denotes |
culture |
T18712 |
1076-1081 |
VBN |
denotes |
added |
T18713 |
1067-1070 |
VBD |
denotes |
was |
T18714 |
1071-1075 |
RB |
denotes |
then |
T18715 |
1082-1084 |
IN |
denotes |
to |
T18716 |
1085-1089 |
DT |
denotes |
each |
T18717 |
1090-1094 |
NN |
denotes |
well |
T18718 |
1094-1095 |
. |
denotes |
. |
T18719 |
1095-1195 |
sentence |
denotes |
In cultures with WT DRG and control beads, 10% serum was added to ensure viability of the explants. |
T18720 |
1096-1098 |
IN |
denotes |
In |
T18721 |
1153-1158 |
VBN |
denotes |
added |
T18722 |
1099-1107 |
NNS |
denotes |
cultures |
T18723 |
1108-1112 |
IN |
denotes |
with |
T18724 |
1113-1115 |
NN |
denotes |
WT |
T18725 |
1116-1119 |
NN |
denotes |
DRG |
T18726 |
1120-1123 |
CC |
denotes |
and |
T18727 |
1124-1131 |
NN |
denotes |
control |
T18728 |
1132-1137 |
NNS |
denotes |
beads |
T18729 |
1137-1139 |
, |
denotes |
, |
T18730 |
1139-1141 |
CD |
denotes |
10 |
T18731 |
1141-1142 |
NN |
denotes |
% |
T18732 |
1143-1148 |
NN |
denotes |
serum |
T18733 |
1149-1152 |
VBD |
denotes |
was |
T18734 |
1159-1161 |
TO |
denotes |
to |
T18735 |
1162-1168 |
VB |
denotes |
ensure |
T18736 |
1169-1178 |
NN |
denotes |
viability |
T18737 |
1179-1181 |
IN |
denotes |
of |
T18738 |
1182-1185 |
DT |
denotes |
the |
T18739 |
1186-1194 |
NNS |
denotes |
explants |
T18740 |
1194-1195 |
. |
denotes |
. |
T18741 |
1195-1313 |
sentence |
denotes |
TrkC-Fc (Regeneron Pharmaceuticals, Tarrytown, New York, United States) was added (20 μg/ml) into the culture medium. |
T18742 |
1196-1200 |
NN |
denotes |
TrkC |
T18743 |
1201-1203 |
NN |
denotes |
Fc |
T18744 |
1200-1201 |
HYPH |
denotes |
- |
T18745 |
1272-1277 |
VBN |
denotes |
added |
T18746 |
1204-1205 |
-LRB- |
denotes |
( |
T18747 |
1215-1230 |
NNP |
denotes |
Pharmaceuticals |
T18748 |
1205-1214 |
NNP |
denotes |
Regeneron |
T18749 |
1230-1232 |
, |
denotes |
, |
T18750 |
1232-1241 |
NNP |
denotes |
Tarrytown |
T18751 |
1241-1243 |
, |
denotes |
, |
T18752 |
1243-1246 |
NNP |
denotes |
New |
T18753 |
1247-1251 |
NNP |
denotes |
York |
T18754 |
1251-1253 |
, |
denotes |
, |
T18755 |
1253-1259 |
NNP |
denotes |
United |
T18756 |
1260-1266 |
NNP |
denotes |
States |
T18757 |
1266-1267 |
-RRB- |
denotes |
) |
T18758 |
1268-1271 |
VBD |
denotes |
was |
T18759 |
1278-1279 |
-LRB- |
denotes |
( |
T18760 |
1282-1284 |
NN |
denotes |
μg |
T18761 |
1279-1281 |
CD |
denotes |
20 |
T18762 |
1284-1285 |
SYM |
denotes |
/ |
T18763 |
1285-1287 |
NN |
denotes |
ml |
T18764 |
1287-1288 |
-RRB- |
denotes |
) |
T18765 |
1289-1293 |
IN |
denotes |
into |
T18766 |
1294-1297 |
DT |
denotes |
the |
T18767 |
1306-1312 |
NN |
denotes |
medium |
T18768 |
1298-1305 |
NN |
denotes |
culture |
T18769 |
1312-1313 |
. |
denotes |
. |
R5357 |
T18468 |
T18469 |
advmod |
In,vitro |
R5358 |
T18469 |
T18470 |
amod |
vitro,assay |
R5359 |
T18471 |
T18470 |
compound |
co-culture,assay |
R5360 |
T18472 |
T18470 |
punct |
.,assay |
R5361 |
T18474 |
T18475 |
compound |
DRG,explants |
R5362 |
T18475 |
T18476 |
nsubjpass |
explants,derived |
R5363 |
T18477 |
T18476 |
auxpass |
were,derived |
R5364 |
T18478 |
T18476 |
prep |
from,derived |
R5365 |
T18479 |
T18480 |
nmod |
Swiss,Webster |
R5366 |
T18480 |
T18481 |
nmod |
Webster,embryos |
R5367 |
T18481 |
T18478 |
pobj |
embryos,from |
R5368 |
T18482 |
T18480 |
punct |
", ",Webster |
R5369 |
T18483 |
T18484 |
npadvmod |
Bax,null |
R5370 |
T18484 |
T18480 |
conj |
null,Webster |
R5371 |
T18485 |
T18484 |
punct |
", ",null |
R5372 |
T18486 |
T18484 |
cc |
and,null |
R5373 |
T18487 |
T18488 |
npadvmod |
p75,null |
R5374 |
T18488 |
T18484 |
conj |
null,null |
R5375 |
T18489 |
T18481 |
compound |
mouse,embryos |
R5376 |
T18490 |
T18491 |
punct |
(,E13 |
R5377 |
T18491 |
T18481 |
parataxis |
E13,embryos |
R5378 |
T18492 |
T18491 |
punct |
),E13 |
R5379 |
T18493 |
T18476 |
punct |
.,derived |
R5380 |
T18495 |
T18496 |
prep |
For,dissected |
R5381 |
T18497 |
T18498 |
compound |
collagen,matrix |
R5382 |
T18498 |
T18499 |
compound |
matrix,assays |
R5383 |
T18499 |
T18495 |
pobj |
assays,For |
R5384 |
T18500 |
T18496 |
punct |
", ",dissected |
R5385 |
T18501 |
T18496 |
nsubjpass |
DRGs,dissected |
R5386 |
T18502 |
T18496 |
auxpass |
were,dissected |
R5387 |
T18503 |
T18496 |
prt |
out,dissected |
R5388 |
T18504 |
T18496 |
prep |
under,dissected |
R5389 |
T18505 |
T18506 |
det |
a,stereomicroscope |
R5390 |
T18506 |
T18504 |
pobj |
stereomicroscope,under |
R5391 |
T18507 |
T18496 |
advcl |
using,dissected |
R5392 |
T18508 |
T18509 |
compound |
tungsten,needles |
R5393 |
T18509 |
T18507 |
dobj |
needles,using |
R5394 |
T18510 |
T18496 |
punct |
.,dissected |
R5395 |
T18512 |
T18513 |
compound |
Collagen,matrix |
R5396 |
T18513 |
T18514 |
nsubjpass |
matrix,prepared |
R5397 |
T18515 |
T18514 |
auxpass |
was,prepared |
R5398 |
T18516 |
T18514 |
prep |
with,prepared |
R5399 |
T18517 |
T18518 |
nummod |
430,μl |
R5400 |
T18518 |
T18516 |
pobj |
μl,with |
R5401 |
T18519 |
T18518 |
prep |
of,μl |
R5402 |
T18520 |
T18519 |
pobj |
collagen,of |
R5403 |
T18521 |
T18522 |
punct |
(,Sigma |
R5404 |
T18522 |
T18518 |
parataxis |
Sigma,μl |
R5405 |
T18523 |
T18524 |
nummod |
3,mg |
R5406 |
T18524 |
T18522 |
dep |
mg,Sigma |
R5407 |
T18525 |
T18526 |
punct |
/,ml |
R5408 |
T18526 |
T18524 |
prep |
ml,mg |
R5409 |
T18527 |
T18524 |
acl |
dissolved,mg |
R5410 |
T18528 |
T18527 |
prep |
in,dissolved |
R5411 |
T18529 |
T18530 |
nummod |
0.1,M |
R5412 |
T18530 |
T18531 |
nmod |
M,acid |
R5413 |
T18531 |
T18528 |
pobj |
acid,in |
R5414 |
T18532 |
T18531 |
amod |
acetic,acid |
R5415 |
T18533 |
T18522 |
punct |
", ",Sigma |
R5416 |
T18534 |
T18522 |
punct |
),Sigma |
R5417 |
T18535 |
T18518 |
punct |
", ",μl |
R5418 |
T18536 |
T18537 |
nummod |
50,μl |
R5419 |
T18537 |
T18518 |
conj |
μl,μl |
R5420 |
T18538 |
T18537 |
prep |
of,μl |
R5421 |
T18539 |
T18540 |
nummod |
10,DMEM |
R5422 |
T18540 |
T18542 |
compound |
DMEM,medium |
R5423 |
T18541 |
T18540 |
compound |
X,DMEM |
R5424 |
T18542 |
T18538 |
pobj |
medium,of |
R5425 |
T18543 |
T18537 |
punct |
", ",μl |
R5426 |
T18544 |
T18537 |
cc |
and,μl |
R5427 |
T18545 |
T18546 |
nummod |
2.5,μl |
R5428 |
T18546 |
T18537 |
conj |
μl,μl |
R5429 |
T18547 |
T18546 |
prep |
of,μl |
R5430 |
T18548 |
T18549 |
nummod |
0.8,M |
R5431 |
T18549 |
T18550 |
compound |
M,NaCa2 |
R5432 |
T18550 |
T18547 |
pobj |
NaCa2,of |
R5433 |
T18551 |
T18514 |
punct |
", ",prepared |
R5434 |
T18552 |
T18514 |
cc |
and,prepared |
R5435 |
T18553 |
T18554 |
det |
the,pH |
R5436 |
T18554 |
T18555 |
nsubjpass |
pH,adjusted |
R5437 |
T18555 |
T18514 |
conj |
adjusted,prepared |
R5438 |
T18556 |
T18555 |
auxpass |
was,adjusted |
R5439 |
T18557 |
T18555 |
prep |
to,adjusted |
R5440 |
T18558 |
T18557 |
pobj |
7.5,to |
R5441 |
T18559 |
T18555 |
punct |
.,adjusted |
R5442 |
T18561 |
T18562 |
amod |
Individual,explants |
R5443 |
T18562 |
T18564 |
nsubjpass |
explants,placed |
R5444 |
T18563 |
T18562 |
compound |
ganglion,explants |
R5445 |
T18565 |
T18564 |
auxpass |
were,placed |
R5446 |
T18566 |
T18564 |
prep |
in,placed |
R5447 |
T18567 |
T18568 |
nummod |
24,well |
R5448 |
T18568 |
T18570 |
compound |
well,plates |
R5449 |
T18569 |
T18568 |
punct |
-,well |
R5450 |
T18570 |
T18566 |
pobj |
plates,in |
R5451 |
T18571 |
T18564 |
cc |
and,placed |
R5452 |
T18572 |
T18564 |
conj |
covered,placed |
R5453 |
T18573 |
T18572 |
prep |
with,covered |
R5454 |
T18574 |
T18575 |
advmod |
freshly,prepared |
R5455 |
T18575 |
T18576 |
amod |
prepared,collagen |
R5456 |
T18576 |
T18573 |
pobj |
collagen,with |
R5457 |
T18577 |
T18564 |
punct |
.,placed |
R5458 |
T18579 |
T18580 |
compound |
Sepharose,beads |
R5459 |
T18580 |
T18581 |
nsubjpass |
beads,used |
R5460 |
T18582 |
T18580 |
prep |
with,beads |
R5461 |
T18583 |
T18584 |
det |
an,diameter |
R5462 |
T18584 |
T18582 |
pobj |
diameter,with |
R5463 |
T18585 |
T18584 |
amod |
average,diameter |
R5464 |
T18586 |
T18584 |
prep |
of,diameter |
R5465 |
T18587 |
T18588 |
nummod |
150,μm |
R5466 |
T18588 |
T18586 |
pobj |
μm,of |
R5467 |
T18589 |
T18581 |
auxpass |
were,used |
R5468 |
T18590 |
T18581 |
punct |
.,used |
R5469 |
T18592 |
T18593 |
nsubjpass |
Beads,washed |
R5470 |
T18594 |
T18593 |
auxpass |
were,washed |
R5471 |
T18595 |
T18593 |
advmod |
twice,washed |
R5472 |
T18596 |
T18593 |
prep |
with,washed |
R5473 |
T18597 |
T18596 |
pobj |
PBS,with |
R5474 |
T18598 |
T18593 |
punct |
", ",washed |
R5475 |
T18599 |
T18600 |
dep |
air,dried |
R5476 |
T18600 |
T18593 |
conj |
dried,washed |
R5477 |
T18601 |
T18600 |
punct |
", ",dried |
R5478 |
T18602 |
T18600 |
cc |
and,dried |
R5479 |
T18603 |
T18600 |
conj |
loaded,dried |
R5480 |
T18604 |
T18603 |
prep |
with,loaded |
R5481 |
T18605 |
T18606 |
nummod |
10,ng |
R5482 |
T18606 |
T18614 |
nmod |
ng,NT |
R5483 |
T18607 |
T18605 |
punct |
", ",10 |
R5484 |
T18608 |
T18605 |
conj |
20,10 |
R5485 |
T18609 |
T18608 |
punct |
", ",20 |
R5486 |
T18610 |
T18608 |
conj |
50,20 |
R5487 |
T18611 |
T18610 |
punct |
", ",50 |
R5488 |
T18612 |
T18610 |
cc |
or,50 |
R5489 |
T18613 |
T18610 |
conj |
100,50 |
R5490 |
T18614 |
T18604 |
pobj |
NT,with |
R5491 |
T18615 |
T18616 |
punct |
/,μl |
R5492 |
T18616 |
T18606 |
prep |
μl,ng |
R5493 |
T18617 |
T18614 |
punct |
-,NT |
R5494 |
T18618 |
T18614 |
nummod |
3,NT |
R5495 |
T18619 |
T18620 |
punct |
(,Research |
R5496 |
T18620 |
T18614 |
parataxis |
Research,NT |
R5497 |
T18621 |
T18620 |
amod |
Collaborative,Research |
R5498 |
T18622 |
T18620 |
punct |
", ",Research |
R5499 |
T18623 |
T18620 |
npadvmod |
Chemicon,Research |
R5500 |
T18624 |
T18620 |
punct |
),Research |
R5501 |
T18625 |
T18603 |
prep |
at,loaded |
R5502 |
T18626 |
T18627 |
nummod |
4,°C |
R5503 |
T18627 |
T18625 |
pobj |
°C,at |
R5504 |
T18628 |
T18603 |
advmod |
overnight,loaded |
R5505 |
T18629 |
T18603 |
prep |
with,loaded |
R5506 |
T18630 |
T18631 |
amod |
constant,shaking |
R5507 |
T18631 |
T18629 |
pobj |
shaking,with |
R5508 |
T18632 |
T18593 |
punct |
.,washed |
R5509 |
T18634 |
T18635 |
prep |
For,loaded |
R5510 |
T18636 |
T18637 |
amod |
negative,control |
R5511 |
T18637 |
T18634 |
pobj |
control,For |
R5512 |
T18638 |
T18635 |
punct |
", ",loaded |
R5513 |
T18639 |
T18635 |
nsubjpass |
beads,loaded |
R5514 |
T18640 |
T18635 |
auxpass |
were,loaded |
R5515 |
T18641 |
T18635 |
prep |
with,loaded |
R5516 |
T18642 |
T18641 |
pobj |
BSA,with |
R5517 |
T18643 |
T18644 |
punct |
(,ng |
R5518 |
T18644 |
T18642 |
parataxis |
ng,BSA |
R5519 |
T18645 |
T18646 |
compound |
10,100 |
R5520 |
T18646 |
T18644 |
nummod |
100,ng |
R5521 |
T18647 |
T18646 |
punct |
–,100 |
R5522 |
T18648 |
T18649 |
punct |
/,μl |
R5523 |
T18649 |
T18644 |
prep |
μl,ng |
R5524 |
T18650 |
T18644 |
punct |
),ng |
R5525 |
T18651 |
T18642 |
cc |
or,BSA |
R5526 |
T18652 |
T18642 |
conj |
PBS,BSA |
R5527 |
T18653 |
T18635 |
punct |
.,loaded |
R5528 |
T18655 |
T18656 |
preconj |
Either,bead |
R5529 |
T18656 |
T18662 |
nsubjpass |
bead,implanted |
R5530 |
T18657 |
T18656 |
det |
a,bead |
R5531 |
T18658 |
T18656 |
amod |
single,bead |
R5532 |
T18659 |
T18660 |
npadvmod |
neurotrophin,loaded |
R5533 |
T18660 |
T18656 |
amod |
loaded,bead |
R5534 |
T18661 |
T18660 |
punct |
-,loaded |
R5535 |
T18663 |
T18656 |
cc |
or,bead |
R5536 |
T18664 |
T18665 |
det |
a,bead |
R5537 |
T18665 |
T18656 |
conj |
bead,bead |
R5538 |
T18666 |
T18665 |
amod |
single,bead |
R5539 |
T18667 |
T18668 |
npadvmod |
BSA,loaded |
R5540 |
T18668 |
T18665 |
amod |
loaded,bead |
R5541 |
T18669 |
T18667 |
punct |
(,BSA |
R5542 |
T18670 |
T18667 |
cc |
or,BSA |
R5543 |
T18671 |
T18667 |
conj |
PBS,BSA |
R5544 |
T18672 |
T18671 |
punct |
),PBS |
R5545 |
T18673 |
T18668 |
punct |
–,loaded |
R5546 |
T18674 |
T18662 |
auxpass |
was,implanted |
R5547 |
T18675 |
T18676 |
quantmod |
about,"1,200" |
R5548 |
T18676 |
T18679 |
nummod |
"1,200",μm |
R5549 |
T18677 |
T18676 |
compound |
200,"1,200" |
R5550 |
T18678 |
T18676 |
punct |
–,"1,200" |
R5551 |
T18679 |
T18680 |
npadvmod |
μm,away |
R5552 |
T18680 |
T18662 |
advmod |
away,implanted |
R5553 |
T18681 |
T18680 |
prep |
from,away |
R5554 |
T18682 |
T18683 |
det |
the,explant |
R5555 |
T18683 |
T18681 |
pobj |
explant,from |
R5556 |
T18684 |
T18683 |
compound |
ganglion,explant |
R5557 |
T18685 |
T18662 |
punct |
.,implanted |
R5558 |
T18687 |
T18688 |
npadvmod |
Collagen,embedded |
R5559 |
T18688 |
T18690 |
amod |
embedded,cultures |
R5560 |
T18689 |
T18688 |
punct |
-,embedded |
R5561 |
T18690 |
T18691 |
nsubjpass |
cultures,placed |
R5562 |
T18692 |
T18691 |
auxpass |
were,placed |
R5563 |
T18693 |
T18691 |
advmod |
then,placed |
R5564 |
T18694 |
T18691 |
prep |
at,placed |
R5565 |
T18695 |
T18696 |
nummod |
33,°C |
R5566 |
T18696 |
T18694 |
pobj |
°C,at |
R5567 |
T18697 |
T18691 |
prep |
for,placed |
R5568 |
T18698 |
T18699 |
nummod |
15,min |
R5569 |
T18699 |
T18697 |
pobj |
min,for |
R5570 |
T18700 |
T18701 |
mark |
for,harden |
R5571 |
T18701 |
T18691 |
advcl |
harden,placed |
R5572 |
T18702 |
T18703 |
det |
the,matrix |
R5573 |
T18703 |
T18701 |
nsubj |
matrix,harden |
R5574 |
T18704 |
T18701 |
aux |
to,harden |
R5575 |
T18705 |
T18691 |
punct |
.,placed |
R5576 |
T18707 |
T18708 |
npadvmod |
Serum,free |
R5577 |
T18708 |
T18710 |
amod |
free,medium |
R5578 |
T18709 |
T18708 |
punct |
-,free |
R5579 |
T18710 |
T18712 |
nsubjpass |
medium,added |
R5580 |
T18711 |
T18710 |
compound |
culture,medium |
R5581 |
T18713 |
T18712 |
auxpass |
was,added |
R5582 |
T18714 |
T18712 |
advmod |
then,added |
R5583 |
T18715 |
T18712 |
prep |
to,added |
R5584 |
T18716 |
T18717 |
det |
each,well |
R5585 |
T18717 |
T18715 |
pobj |
well,to |
R5586 |
T18718 |
T18712 |
punct |
.,added |
R5587 |
T18720 |
T18721 |
prep |
In,added |
R5588 |
T18722 |
T18720 |
pobj |
cultures,In |
R5589 |
T18723 |
T18722 |
prep |
with,cultures |
R5590 |
T18724 |
T18725 |
compound |
WT,DRG |
R5591 |
T18725 |
T18723 |
pobj |
DRG,with |
R5592 |
T18726 |
T18725 |
cc |
and,DRG |
R5593 |
T18727 |
T18728 |
compound |
control,beads |
R5594 |
T18728 |
T18725 |
conj |
beads,DRG |
R5595 |
T18729 |
T18721 |
punct |
", ",added |
R5596 |
T18730 |
T18731 |
nummod |
10,% |
R5597 |
T18731 |
T18732 |
compound |
%,serum |
R5598 |
T18732 |
T18721 |
nsubjpass |
serum,added |
R5599 |
T18733 |
T18721 |
auxpass |
was,added |
R5600 |
T18734 |
T18735 |
aux |
to,ensure |
R5601 |
T18735 |
T18721 |
advcl |
ensure,added |
R5602 |
T18736 |
T18735 |
dobj |
viability,ensure |
R5603 |
T18737 |
T18736 |
prep |
of,viability |
R5604 |
T18738 |
T18739 |
det |
the,explants |
R5605 |
T18739 |
T18737 |
pobj |
explants,of |
R5606 |
T18740 |
T18721 |
punct |
.,added |
R5607 |
T18742 |
T18743 |
compound |
TrkC,Fc |
R5608 |
T18743 |
T18745 |
nsubjpass |
Fc,added |
R5609 |
T18744 |
T18743 |
punct |
-,Fc |
R5610 |
T18746 |
T18747 |
punct |
(,Pharmaceuticals |
R5611 |
T18747 |
T18743 |
parataxis |
Pharmaceuticals,Fc |
R5612 |
T18748 |
T18747 |
compound |
Regeneron,Pharmaceuticals |
R5613 |
T18749 |
T18747 |
punct |
", ",Pharmaceuticals |
R5614 |
T18750 |
T18747 |
npadvmod |
Tarrytown,Pharmaceuticals |
R5615 |
T18751 |
T18747 |
punct |
", ",Pharmaceuticals |
R5616 |
T18752 |
T18753 |
compound |
New,York |
R5617 |
T18753 |
T18747 |
npadvmod |
York,Pharmaceuticals |
R5618 |
T18754 |
T18747 |
punct |
", ",Pharmaceuticals |
R5619 |
T18755 |
T18756 |
compound |
United,States |
R5620 |
T18756 |
T18747 |
npadvmod |
States,Pharmaceuticals |
R5621 |
T18757 |
T18747 |
punct |
),Pharmaceuticals |
R5622 |
T18758 |
T18745 |
auxpass |
was,added |
R5623 |
T18759 |
T18760 |
punct |
(,μg |
R5624 |
T18760 |
T18745 |
parataxis |
μg,added |
R5625 |
T18761 |
T18760 |
nummod |
20,μg |
R5626 |
T18762 |
T18763 |
punct |
/,ml |
R5627 |
T18763 |
T18760 |
prep |
ml,μg |
R5628 |
T18764 |
T18760 |
punct |
),μg |
R5629 |
T18765 |
T18745 |
prep |
into,added |
R5630 |
T18766 |
T18767 |
det |
the,medium |
R5631 |
T18767 |
T18765 |
pobj |
medium,into |
R5632 |
T18768 |
T18767 |
compound |
culture,medium |
R5633 |
T18769 |
T18745 |
punct |
.,added |