PMC:529315 / 29586-30996 JSONTXT

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T16172","span":{"begin":11,"end":14},"obj":"PR_EXT:000002193"},{"id":"T16173","span":{"begin":18,"end":25},"obj":"PATO_UBERON_EXT:female_or_bearer_of_femaleness"},{"id":"T16174","span":{"begin":108,"end":112},"obj":"PR_EXT:000011459"},{"id":"T16175","span":{"begin":126,"end":131},"obj":"PATO_UBERON_EXT:male_or_bearer_of_maleness"},{"id":"T16176","span":{"begin":187,"end":194},"obj":"NCBITaxon:33208"},{"id":"T16177","span":{"begin":232,"end":239},"obj":"NCBITaxon:33208"},{"id":"T16178","span":{"begin":262,"end":267},"obj":"SO_EXT:0000704"},{"id":"T16179","span":{"begin":273,"end":277},"obj":"GO_EXT:breeding"},{"id":"T16180","span":{"begin":304,"end":311},"obj":"SO_EXT:0000112"},{"id":"T16181","span":{"begin":325,"end":328},"obj":"PR_EXT:000002193"},{"id":"T16182","span":{"begin":447,"end":454},"obj":"SO_EXT:0000112"},{"id":"T16183","span":{"begin":468,"end":472},"obj":"PR_EXT:000011459"},{"id":"T16184","span":{"begin":630,"end":632},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T16185","span":{"begin":634,"end":637},"obj":"PR_EXT:000002193"},{"id":"T16186","span":{"begin":646,"end":650},"obj":"PR_EXT:000011459"},{"id":"T16187","span":{"begin":703,"end":706},"obj":"PR_EXT:000002193"},{"id":"T16188","span":{"begin":707,"end":711},"obj":"PR_EXT:000011459"},{"id":"T16189","span":{"begin":722,"end":726},"obj":"NCBITaxon:10088"},{"id":"T16190","span":{"begin":764,"end":768},"obj":"NCBITaxon_UBERON_EXT:pup"},{"id":"T16191","span":{"begin":774,"end":777},"obj":"PR_EXT:000001405"},{"id":"T16192","span":{"begin":854,"end":858},"obj":"UBERON:0002415"},{"id":"T16193","span":{"begin":859,"end":862},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T16194","span":{"begin":875,"end":882},"obj":"NCBITaxon:33208"},{"id":"T16195","span":{"begin":893,"end":900},"obj":"SO_EXT:0000112"},{"id":"T16196","span":{"begin":983,"end":986},"obj":"CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase"},{"id":"T16197","span":{"begin":987,"end":994},"obj":"SO_EXT:0000112"},{"id":"T16198","span":{"begin":1080,"end":1083},"obj":"PR_EXT:000001405"},{"id":"T16199","span":{"begin":1096,"end":1105},"obj":"UBERON:0000922"},{"id":"T16200","span":{"begin":1126,"end":1130},"obj":"UBERON:0010148"},{"id":"T16201","span":{"begin":1286,"end":1292},"obj":"NCBITaxon:33208"},{"id":"T16202","span":{"begin":1402,"end":1409},"obj":"NCBITaxon:33208"}],"text":"We crossed Bax KO females on a C57BL/6 background (Jackson Laboratory, Bar Harbor, Maine, United States) to NT-3 heterozygote males on a 129 Sv background to generate double heterozygote animals. Progeny was genotyped with PCR, and animals heterozygous for both genes were bred to obtain the double KOs. Primers used for the Bax locus were R661, GTT GAC CAG AGT GGC GTA GG; R662, CCG CTT CCA TTG CTC AGC GG; and R663, GAG CTG ATC AGA ACC ATC ATG. Primers used for the NT-3 locus were NT3A, CGT GGT GAG GTT CTA TTG GCT AC; NT3B, CAG AGC ACC CTG CCC AAA GCA GAG; NT3R, CCT TGA CAA TAC TGA ATG CC; and NEOF, GGG AAC TTC CTG ACT AGG. WT, Bax KO, and NT-3 KO littermates were used as controls. A total of 11 Bax/NT-3 double KO mice were analyzed, of these nine were P0 pups. The p75 colony on a 129 S1 background was received from Jackson Laboratory. We used tail DNA to genotype animals using the primers IMR0013, CTT GGG TGG AGA GGC TAT TC; IMR0014, AGG TGA GAT GAC AGG AGA TC (generic neo primers); IMR0710, TGT TAC GTT CTC TGA CGT GGT GAG; and IMR0711, TCA GCC CAG GGT GTG CAC TC (p75 locus). For embryonic experiments, day of plug positivity was considered E0. All of the protocols used in this study were approved by the Louisiana State University Health Sciences Center Institutional Animal Care and Use Committee and conformed to the National Institutes of Health guidelines for use of experimental animals."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T16148","span":{"begin":11,"end":14},"obj":"PR:000002193"},{"id":"T16149","span":{"begin":108,"end":112},"obj":"PR:000011459"},{"id":"T16150","span":{"begin":187,"end":194},"obj":"NCBITaxon:33208"},{"id":"T16151","span":{"begin":232,"end":239},"obj":"NCBITaxon:33208"},{"id":"T16152","span":{"begin":262,"end":267},"obj":"SO:0000704"},{"id":"T16153","span":{"begin":304,"end":311},"obj":"SO:0000112"},{"id":"T16154","span":{"begin":325,"end":328},"obj":"PR:000002193"},{"id":"T16155","span":{"begin":447,"end":454},"obj":"SO:0000112"},{"id":"T16156","span":{"begin":468,"end":472},"obj":"PR:000011459"},{"id":"T16157","span":{"begin":634,"end":637},"obj":"PR:000002193"},{"id":"T16158","span":{"begin":646,"end":650},"obj":"PR:000011459"},{"id":"T16159","span":{"begin":703,"end":706},"obj":"PR:000002193"},{"id":"T16160","span":{"begin":707,"end":711},"obj":"PR:000011459"},{"id":"T16161","span":{"begin":722,"end":726},"obj":"NCBITaxon:10088"},{"id":"T16162","span":{"begin":774,"end":777},"obj":"PR:000001405"},{"id":"T16163","span":{"begin":854,"end":858},"obj":"UBERON:0002415"},{"id":"T16164","span":{"begin":875,"end":882},"obj":"NCBITaxon:33208"},{"id":"T16165","span":{"begin":893,"end":900},"obj":"SO:0000112"},{"id":"T16166","span":{"begin":987,"end":994},"obj":"SO:0000112"},{"id":"T16167","span":{"begin":1080,"end":1083},"obj":"PR:000001405"},{"id":"T16168","span":{"begin":1096,"end":1105},"obj":"UBERON:0000922"},{"id":"T16169","span":{"begin":1126,"end":1130},"obj":"UBERON:0010148"},{"id":"T16170","span":{"begin":1286,"end":1292},"obj":"NCBITaxon:33208"},{"id":"T16171","span":{"begin":1402,"end":1409},"obj":"NCBITaxon:33208"}],"text":"We crossed Bax KO females on a C57BL/6 background (Jackson Laboratory, Bar Harbor, Maine, United States) to NT-3 heterozygote males on a 129 Sv background to generate double heterozygote animals. Progeny was genotyped with PCR, and animals heterozygous for both genes were bred to obtain the double KOs. Primers used for the Bax locus were R661, GTT GAC CAG AGT GGC GTA GG; R662, CCG CTT CCA TTG CTC AGC GG; and R663, GAG CTG ATC AGA ACC ATC ATG. Primers used for the NT-3 locus were NT3A, CGT GGT GAG GTT CTA TTG GCT AC; NT3B, CAG AGC ACC CTG CCC AAA GCA GAG; NT3R, CCT TGA CAA TAC TGA ATG CC; and NEOF, GGG AAC TTC CTG ACT AGG. WT, Bax KO, and NT-3 KO littermates were used as controls. A total of 11 Bax/NT-3 double KO mice were analyzed, of these nine were P0 pups. The p75 colony on a 129 S1 background was received from Jackson Laboratory. We used tail DNA to genotype animals using the primers IMR0013, CTT GGG TGG AGA GGC TAT TC; IMR0014, AGG TGA GAT GAC AGG AGA TC (generic neo primers); IMR0710, TGT TAC GTT CTC TGA CGT GGT GAG; and IMR0711, TCA GCC CAG GGT GTG CAC TC (p75 locus). For embryonic experiments, day of plug positivity was considered E0. All of the protocols used in this study were approved by the Louisiana State University Health Sciences Center Institutional Animal Care and Use Committee and conformed to the National Institutes of Health guidelines for use of experimental animals."}

{"project":"craft-sa-dev","denotations":[{"id":"T16209","span":{"begin":0,"end":2},"obj":"PRP"},{"id":"T16210","span":{"begin":3,"end":10},"obj":"VBD"},{"id":"T16211","span":{"begin":11,"end":14},"obj":"NN"},{"id":"T16212","span":{"begin":15,"end":17},"obj":"NN"},{"id":"T16213","span":{"begin":18,"end":25},"obj":"NNS"},{"id":"T16214","span":{"begin":26,"end":28},"obj":"IN"},{"id":"T16215","span":{"begin":29,"end":30},"obj":"DT"},{"id":"T16216","span":{"begin":39,"end":49},"obj":"NN"},{"id":"T16217","span":{"begin":31,"end":36},"obj":"NN"},{"id":"T16218","span":{"begin":36,"end":37},"obj":"HYPH"},{"id":"T16219","span":{"begin":37,"end":38},"obj":"CD"},{"id":"T16220","span":{"begin":50,"end":51},"obj":"-LRB-"},{"id":"T16221","span":{"begin":59,"end":69},"obj":"NNP"},{"id":"T16222","span":{"begin":51,"end":58},"obj":"NNP"},{"id":"T16223","span":{"begin":69,"end":71},"obj":","},{"id":"T16224","span":{"begin":71,"end":74},"obj":"NNP"},{"id":"T16225","span":{"begin":75,"end":81},"obj":"NNP"},{"id":"T16226","span":{"begin":81,"end":83},"obj":","},{"id":"T16227","span":{"begin":83,"end":88},"obj":"NNP"},{"id":"T16228","span":{"begin":88,"end":90},"obj":","},{"id":"T16229","span":{"begin":90,"end":96},"obj":"NNP"},{"id":"T16230","span":{"begin":97,"end":103},"obj":"NNP"},{"id":"T16231","span":{"begin":103,"end":104},"obj":"-RRB-"},{"id":"T16232","span":{"begin":105,"end":107},"obj":"IN"},{"id":"T16233","span":{"begin":108,"end":110},"obj":"NN"},{"id":"T16234","span":{"begin":113,"end":125},"obj":"NN"},{"id":"T16235","span":{"begin":110,"end":111},"obj":"HYPH"},{"id":"T16236","span":{"begin":111,"end":112},"obj":"CD"},{"id":"T16237","span":{"begin":126,"end":131},"obj":"NNS"},{"id":"T16238","span":{"begin":132,"end":134},"obj":"IN"},{"id":"T16239","span":{"begin":135,"end":136},"obj":"DT"},{"id":"T16240","span":{"begin":144,"end":154},"obj":"NN"},{"id":"T16241","span":{"begin":137,"end":140},"obj":"CD"},{"id":"T16242","span":{"begin":141,"end":143},"obj":"NN"},{"id":"T16243","span":{"begin":155,"end":157},"obj":"TO"},{"id":"T16244","span":{"begin":158,"end":166},"obj":"VB"},{"id":"T16245","span":{"begin":167,"end":173},"obj":"JJ"},{"id":"T16246","span":{"begin":174,"end":186},"obj":"NN"},{"id":"T16247","span":{"begin":187,"end":194},"obj":"NNS"},{"id":"T16248","span":{"begin":194,"end":195},"obj":"."},{"id":"T16249","span":{"begin":195,"end":303},"obj":"sentence"},{"id":"T16250","span":{"begin":196,"end":203},"obj":"NN"},{"id":"T16251","span":{"begin":208,"end":217},"obj":"VBN"},{"id":"T16252","span":{"begin":204,"end":207},"obj":"VBD"},{"id":"T16253","span":{"begin":218,"end":222},"obj":"IN"},{"id":"T16254","span":{"begin":223,"end":226},"obj":"NN"},{"id":"T16255","span":{"begin":226,"end":228},"obj":","},{"id":"T16256","span":{"begin":228,"end":231},"obj":"CC"},{"id":"T16257","span":{"begin":232,"end":239},"obj":"NNS"},{"id":"T16258","span":{"begin":273,"end":277},"obj":"VBN"},{"id":"T16259","span":{"begin":240,"end":252},"obj":"JJ"},{"id":"T16260","span":{"begin":253,"end":256},"obj":"IN"},{"id":"T16261","span":{"begin":257,"end":261},"obj":"DT"},{"id":"T16262","span":{"begin":262,"end":267},"obj":"NNS"},{"id":"T16263","span":{"begin":268,"end":272},"obj":"VBD"},{"id":"T16264","span":{"begin":278,"end":280},"obj":"TO"},{"id":"T16265","span":{"begin":281,"end":287},"obj":"VB"},{"id":"T16266","span":{"begin":288,"end":291},"obj":"DT"},{"id":"T16267","span":{"begin":299,"end":302},"obj":"NNS"},{"id":"T16268","span":{"begin":292,"end":298},"obj":"JJ"},{"id":"T16269","span":{"begin":302,"end":303},"obj":"."},{"id":"T16270","span":{"begin":303,"end":446},"obj":"sentence"},{"id":"T16271","span":{"begin":304,"end":311},"obj":"NNS"},{"id":"T16272","span":{"begin":335,"end":339},"obj":"VBD"},{"id":"T16273","span":{"begin":312,"end":316},"obj":"VBN"},{"id":"T16274","span":{"begin":317,"end":320},"obj":"IN"},{"id":"T16275","span":{"begin":321,"end":324},"obj":"DT"},{"id":"T16276","span":{"begin":329,"end":334},"obj":"NN"},{"id":"T16277","span":{"begin":325,"end":328},"obj":"NN"},{"id":"T16278","span":{"begin":340,"end":344},"obj":"NN"},{"id":"T16279","span":{"begin":344,"end":346},"obj":","},{"id":"T16280","span":{"begin":346,"end":349},"obj":"NN"},{"id":"T16281","span":{"begin":370,"end":372},"obj":"NN"},{"id":"T16282","span":{"begin":350,"end":353},"obj":"NN"},{"id":"T16283","span":{"begin":354,"end":357},"obj":"NN"},{"id":"T16284","span":{"begin":358,"end":361},"obj":"NN"},{"id":"T16285","span":{"begin":362,"end":365},"obj":"NN"},{"id":"T16286","span":{"begin":366,"end":369},"obj":"NN"},{"id":"T16287","span":{"begin":372,"end":373},"obj":":"},{"id":"T16288","span":{"begin":374,"end":378},"obj":"NN"},{"id":"T16289","span":{"begin":378,"end":380},"obj":","},{"id":"T16290","span":{"begin":380,"end":383},"obj":"NN"},{"id":"T16291","span":{"begin":404,"end":406},"obj":"NN"},{"id":"T16292","span":{"begin":384,"end":387},"obj":"NN"},{"id":"T16293","span":{"begin":388,"end":391},"obj":"NN"},{"id":"T16294","span":{"begin":392,"end":395},"obj":"NN"},{"id":"T16295","span":{"begin":396,"end":399},"obj":"NN"},{"id":"T16296","span":{"begin":400,"end":403},"obj":"NN"},{"id":"T16297","span":{"begin":406,"end":407},"obj":":"},{"id":"T16298","span":{"begin":408,"end":411},"obj":"CC"},{"id":"T16299","span":{"begin":412,"end":416},"obj":"NN"},{"id":"T16300","span":{"begin":416,"end":418},"obj":","},{"id":"T16301","span":{"begin":418,"end":421},"obj":"NN"},{"id":"T16302","span":{"begin":442,"end":445},"obj":"NN"},{"id":"T16303","span":{"begin":422,"end":425},"obj":"NN"},{"id":"T16304","span":{"begin":426,"end":429},"obj":"NN"},{"id":"T16305","span":{"begin":430,"end":433},"obj":"NN"},{"id":"T16306","span":{"begin":434,"end":437},"obj":"NN"},{"id":"T16307","span":{"begin":438,"end":441},"obj":"NN"},{"id":"T16308","span":{"begin":445,"end":446},"obj":"."},{"id":"T16309","span":{"begin":446,"end":629},"obj":"sentence"},{"id":"T16310","span":{"begin":447,"end":454},"obj":"NNS"},{"id":"T16311","span":{"begin":479,"end":483},"obj":"VBD"},{"id":"T16312","span":{"begin":455,"end":459},"obj":"VBN"},{"id":"T16313","span":{"begin":460,"end":463},"obj":"IN"},{"id":"T16314","span":{"begin":464,"end":467},"obj":"DT"},{"id":"T16315","span":{"begin":473,"end":478},"obj":"NN"},{"id":"T16316","span":{"begin":468,"end":470},"obj":"NN"},{"id":"T16317","span":{"begin":470,"end":471},"obj":"HYPH"},{"id":"T16318","span":{"begin":471,"end":472},"obj":"CD"},{"id":"T16319","span":{"begin":484,"end":488},"obj":"NN"},{"id":"T16320","span":{"begin":488,"end":490},"obj":","},{"id":"T16321","span":{"begin":490,"end":493},"obj":"NN"},{"id":"T16322","span":{"begin":518,"end":520},"obj":"NN"},{"id":"T16323","span":{"begin":494,"end":497},"obj":"NN"},{"id":"T16324","span":{"begin":498,"end":501},"obj":"NN"},{"id":"T16325","span":{"begin":502,"end":505},"obj":"NN"},{"id":"T16326","span":{"begin":506,"end":509},"obj":"NN"},{"id":"T16327","span":{"begin":510,"end":513},"obj":"NN"},{"id":"T16328","span":{"begin":514,"end":517},"obj":"NN"},{"id":"T16329","span":{"begin":520,"end":521},"obj":":"},{"id":"T16330","span":{"begin":522,"end":526},"obj":"NN"},{"id":"T16331","span":{"begin":526,"end":528},"obj":","},{"id":"T16332","span":{"begin":528,"end":531},"obj":"NN"},{"id":"T16333","span":{"begin":556,"end":559},"obj":"NN"},{"id":"T16334","span":{"begin":532,"end":535},"obj":"NN"},{"id":"T16335","span":{"begin":536,"end":539},"obj":"NN"},{"id":"T16336","span":{"begin":540,"end":543},"obj":"NN"},{"id":"T16337","span":{"begin":544,"end":547},"obj":"NN"},{"id":"T16338","span":{"begin":548,"end":551},"obj":"NN"},{"id":"T16339","span":{"begin":552,"end":555},"obj":"NN"},{"id":"T16340","span":{"begin":559,"end":560},"obj":":"},{"id":"T16341","span":{"begin":561,"end":565},"obj":"NN"},{"id":"T16342","span":{"begin":565,"end":567},"obj":","},{"id":"T16343","span":{"begin":567,"end":570},"obj":"NN"},{"id":"T16344","span":{"begin":591,"end":593},"obj":"NN"},{"id":"T16345","span":{"begin":571,"end":574},"obj":"NN"},{"id":"T16346","span":{"begin":575,"end":578},"obj":"NN"},{"id":"T16347","span":{"begin":579,"end":582},"obj":"NN"},{"id":"T16348","span":{"begin":583,"end":586},"obj":"NN"},{"id":"T16349","span":{"begin":587,"end":590},"obj":"NN"},{"id":"T16350","span":{"begin":593,"end":594},"obj":":"},{"id":"T16351","span":{"begin":595,"end":598},"obj":"CC"},{"id":"T16352","span":{"begin":599,"end":603},"obj":"NN"},{"id":"T16353","span":{"begin":603,"end":605},"obj":","},{"id":"T16354","span":{"begin":605,"end":608},"obj":"NN"},{"id":"T16355","span":{"begin":625,"end":628},"obj":"NN"},{"id":"T16356","span":{"begin":609,"end":612},"obj":"NN"},{"id":"T16357","span":{"begin":613,"end":616},"obj":"NN"},{"id":"T16358","span":{"begin":617,"end":620},"obj":"NN"},{"id":"T16359","span":{"begin":621,"end":624},"obj":"NN"},{"id":"T16360","span":{"begin":628,"end":629},"obj":"."},{"id":"T16361","span":{"begin":629,"end":688},"obj":"sentence"},{"id":"T16362","span":{"begin":630,"end":632},"obj":"NN"},{"id":"T16363","span":{"begin":654,"end":665},"obj":"NNS"},{"id":"T16364","span":{"begin":632,"end":634},"obj":","},{"id":"T16365","span":{"begin":634,"end":637},"obj":"NN"},{"id":"T16366","span":{"begin":638,"end":640},"obj":"NN"},{"id":"T16367","span":{"begin":640,"end":642},"obj":","},{"id":"T16368","span":{"begin":642,"end":645},"obj":"CC"},{"id":"T16369","span":{"begin":646,"end":648},"obj":"NN"},{"id":"T16370","span":{"begin":651,"end":653},"obj":"NN"},{"id":"T16371","span":{"begin":648,"end":649},"obj":"HYPH"},{"id":"T16372","span":{"begin":649,"end":650},"obj":"CD"},{"id":"T16373","span":{"begin":671,"end":675},"obj":"VBN"},{"id":"T16374","span":{"begin":666,"end":670},"obj":"VBD"},{"id":"T16375","span":{"begin":676,"end":678},"obj":"IN"},{"id":"T16376","span":{"begin":679,"end":687},"obj":"NNS"},{"id":"T16377","span":{"begin":687,"end":688},"obj":"."},{"id":"T16378","span":{"begin":688,"end":769},"obj":"sentence"},{"id":"T16379","span":{"begin":689,"end":690},"obj":"DT"},{"id":"T16380","span":{"begin":691,"end":696},"obj":"NN"},{"id":"T16381","span":{"begin":732,"end":740},"obj":"VBN"},{"id":"T16382","span":{"begin":697,"end":699},"obj":"IN"},{"id":"T16383","span":{"begin":700,"end":702},"obj":"CD"},{"id":"T16384","span":{"begin":722,"end":726},"obj":"NNS"},{"id":"T16385","span":{"begin":703,"end":706},"obj":"NN"},{"id":"T16386","span":{"begin":719,"end":721},"obj":"NN"},{"id":"T16387","span":{"begin":706,"end":707},"obj":"HYPH"},{"id":"T16388","span":{"begin":707,"end":709},"obj":"NN"},{"id":"T16389","span":{"begin":709,"end":710},"obj":"HYPH"},{"id":"T16390","span":{"begin":710,"end":711},"obj":"CD"},{"id":"T16391","span":{"begin":712,"end":718},"obj":"JJ"},{"id":"T16392","span":{"begin":727,"end":731},"obj":"VBD"},{"id":"T16393","span":{"begin":756,"end":760},"obj":"VBD"},{"id":"T16394","span":{"begin":740,"end":742},"obj":","},{"id":"T16395","span":{"begin":742,"end":744},"obj":"IN"},{"id":"T16396","span":{"begin":745,"end":750},"obj":"DT"},{"id":"T16397","span":{"begin":751,"end":755},"obj":"CD"},{"id":"T16398","span":{"begin":761,"end":763},"obj":"NN"},{"id":"T16399","span":{"begin":764,"end":768},"obj":"NNS"},{"id":"T16400","span":{"begin":768,"end":769},"obj":"."},{"id":"T16401","span":{"begin":769,"end":845},"obj":"sentence"},{"id":"T16402","span":{"begin":770,"end":773},"obj":"DT"},{"id":"T16403","span":{"begin":778,"end":784},"obj":"NN"},{"id":"T16404","span":{"begin":774,"end":777},"obj":"NN"},{"id":"T16405","span":{"begin":812,"end":820},"obj":"VBN"},{"id":"T16406","span":{"begin":785,"end":787},"obj":"IN"},{"id":"T16407","span":{"begin":788,"end":789},"obj":"DT"},{"id":"T16408","span":{"begin":797,"end":807},"obj":"NN"},{"id":"T16409","span":{"begin":790,"end":793},"obj":"CD"},{"id":"T16410","span":{"begin":794,"end":796},"obj":"NN"},{"id":"T16411","span":{"begin":808,"end":811},"obj":"VBD"},{"id":"T16412","span":{"begin":821,"end":825},"obj":"IN"},{"id":"T16413","span":{"begin":826,"end":833},"obj":"NNP"},{"id":"T16414","span":{"begin":834,"end":844},"obj":"NNP"},{"id":"T16415","span":{"begin":844,"end":845},"obj":"."},{"id":"T16416","span":{"begin":845,"end":1091},"obj":"sentence"},{"id":"T16417","span":{"begin":846,"end":848},"obj":"PRP"},{"id":"T16418","span":{"begin":849,"end":853},"obj":"VBD"},{"id":"T16419","span":{"begin":854,"end":858},"obj":"NN"},{"id":"T16420","span":{"begin":859,"end":862},"obj":"NN"},{"id":"T16421","span":{"begin":863,"end":865},"obj":"TO"},{"id":"T16422","span":{"begin":866,"end":874},"obj":"VB"},{"id":"T16423","span":{"begin":875,"end":882},"obj":"NNS"},{"id":"T16424","span":{"begin":883,"end":888},"obj":"VBG"},{"id":"T16425","span":{"begin":889,"end":892},"obj":"DT"},{"id":"T16426","span":{"begin":893,"end":900},"obj":"NNS"},{"id":"T16427","span":{"begin":901,"end":908},"obj":"NN"},{"id":"T16428","span":{"begin":908,"end":910},"obj":","},{"id":"T16429","span":{"begin":910,"end":913},"obj":"NN"},{"id":"T16430","span":{"begin":934,"end":936},"obj":"NN"},{"id":"T16431","span":{"begin":914,"end":917},"obj":"NN"},{"id":"T16432","span":{"begin":918,"end":921},"obj":"NN"},{"id":"T16433","span":{"begin":922,"end":925},"obj":"NN"},{"id":"T16434","span":{"begin":926,"end":929},"obj":"NN"},{"id":"T16435","span":{"begin":930,"end":933},"obj":"NN"},{"id":"T16436","span":{"begin":936,"end":937},"obj":":"},{"id":"T16437","span":{"begin":938,"end":945},"obj":"NN"},{"id":"T16438","span":{"begin":945,"end":947},"obj":","},{"id":"T16439","span":{"begin":947,"end":950},"obj":"NN"},{"id":"T16440","span":{"begin":971,"end":973},"obj":"NN"},{"id":"T16441","span":{"begin":951,"end":954},"obj":"NN"},{"id":"T16442","span":{"begin":955,"end":958},"obj":"NN"},{"id":"T16443","span":{"begin":959,"end":962},"obj":"NN"},{"id":"T16444","span":{"begin":963,"end":966},"obj":"NN"},{"id":"T16445","span":{"begin":967,"end":970},"obj":"NN"},{"id":"T16446","span":{"begin":974,"end":975},"obj":"-LRB-"},{"id":"T16447","span":{"begin":975,"end":982},"obj":"JJ"},{"id":"T16448","span":{"begin":987,"end":994},"obj":"NNS"},{"id":"T16449","span":{"begin":983,"end":986},"obj":"NN"},{"id":"T16450","span":{"begin":994,"end":995},"obj":"-RRB-"},{"id":"T16451","span":{"begin":995,"end":996},"obj":":"},{"id":"T16452","span":{"begin":997,"end":1004},"obj":"NN"},{"id":"T16453","span":{"begin":1004,"end":1006},"obj":","},{"id":"T16454","span":{"begin":1006,"end":1009},"obj":"NN"},{"id":"T16455","span":{"begin":1034,"end":1037},"obj":"NN"},{"id":"T16456","span":{"begin":1010,"end":1013},"obj":"NN"},{"id":"T16457","span":{"begin":1014,"end":1017},"obj":"NN"},{"id":"T16458","span":{"begin":1018,"end":1021},"obj":"NN"},{"id":"T16459","span":{"begin":1022,"end":1025},"obj":"NN"},{"id":"T16460","span":{"begin":1026,"end":1029},"obj":"NN"},{"id":"T16461","span":{"begin":1030,"end":1033},"obj":"NN"},{"id":"T16462","span":{"begin":1037,"end":1038},"obj":":"},{"id":"T16463","span":{"begin":1039,"end":1042},"obj":"CC"},{"id":"T16464","span":{"begin":1043,"end":1050},"obj":"NN"},{"id":"T16465","span":{"begin":1050,"end":1052},"obj":","},{"id":"T16466","span":{"begin":1052,"end":1055},"obj":"NN"},{"id":"T16467","span":{"begin":1076,"end":1078},"obj":"NN"},{"id":"T16468","span":{"begin":1056,"end":1059},"obj":"NN"},{"id":"T16469","span":{"begin":1060,"end":1063},"obj":"NN"},{"id":"T16470","span":{"begin":1064,"end":1067},"obj":"NN"},{"id":"T16471","span":{"begin":1068,"end":1071},"obj":"NN"},{"id":"T16472","span":{"begin":1072,"end":1075},"obj":"NN"},{"id":"T16473","span":{"begin":1079,"end":1080},"obj":"-LRB-"},{"id":"T16474","span":{"begin":1084,"end":1089},"obj":"NN"},{"id":"T16475","span":{"begin":1080,"end":1083},"obj":"NN"},{"id":"T16476","span":{"begin":1089,"end":1090},"obj":"-RRB-"},{"id":"T16477","span":{"begin":1090,"end":1091},"obj":"."},{"id":"T16478","span":{"begin":1091,"end":1160},"obj":"sentence"},{"id":"T16479","span":{"begin":1092,"end":1095},"obj":"IN"},{"id":"T16480","span":{"begin":1146,"end":1156},"obj":"VBN"},{"id":"T16481","span":{"begin":1096,"end":1105},"obj":"JJ"},{"id":"T16482","span":{"begin":1106,"end":1117},"obj":"NNS"},{"id":"T16483","span":{"begin":1117,"end":1119},"obj":","},{"id":"T16484","span":{"begin":1119,"end":1122},"obj":"NN"},{"id":"T16485","span":{"begin":1123,"end":1125},"obj":"IN"},{"id":"T16486","span":{"begin":1126,"end":1130},"obj":"NN"},{"id":"T16487","span":{"begin":1131,"end":1141},"obj":"NN"},{"id":"T16488","span":{"begin":1142,"end":1145},"obj":"VBD"},{"id":"T16489","span":{"begin":1157,"end":1159},"obj":"NN"},{"id":"T16490","span":{"begin":1159,"end":1160},"obj":"."},{"id":"T16491","span":{"begin":1160,"end":1410},"obj":"sentence"},{"id":"T16492","span":{"begin":1161,"end":1164},"obj":"DT"},{"id":"T16493","span":{"begin":1206,"end":1214},"obj":"VBN"},{"id":"T16494","span":{"begin":1165,"end":1167},"obj":"IN"},{"id":"T16495","span":{"begin":1168,"end":1171},"obj":"DT"},{"id":"T16496","span":{"begin":1172,"end":1181},"obj":"NNS"},{"id":"T16497","span":{"begin":1182,"end":1186},"obj":"VBN"},{"id":"T16498","span":{"begin":1187,"end":1189},"obj":"IN"},{"id":"T16499","span":{"begin":1190,"end":1194},"obj":"DT"},{"id":"T16500","span":{"begin":1195,"end":1200},"obj":"NN"},{"id":"T16501","span":{"begin":1201,"end":1205},"obj":"VBD"},{"id":"T16502","span":{"begin":1215,"end":1217},"obj":"IN"},{"id":"T16503","span":{"begin":1218,"end":1221},"obj":"DT"},{"id":"T16504","span":{"begin":1306,"end":1315},"obj":"NNP"},{"id":"T16505","span":{"begin":1222,"end":1231},"obj":"NNP"},{"id":"T16506","span":{"begin":1238,"end":1248},"obj":"NNP"},{"id":"T16507","span":{"begin":1232,"end":1237},"obj":"NNP"},{"id":"T16508","span":{"begin":1265,"end":1271},"obj":"NNP"},{"id":"T16509","span":{"begin":1249,"end":1255},"obj":"NNP"},{"id":"T16510","span":{"begin":1256,"end":1264},"obj":"NNPS"},{"id":"T16511","span":{"begin":1272,"end":1285},"obj":"NNP"},{"id":"T16512","span":{"begin":1286,"end":1292},"obj":"NNP"},{"id":"T16513","span":{"begin":1293,"end":1297},"obj":"NNP"},{"id":"T16514","span":{"begin":1298,"end":1301},"obj":"CC"},{"id":"T16515","span":{"begin":1302,"end":1305},"obj":"NNP"},{"id":"T16516","span":{"begin":1316,"end":1319},"obj":"CC"},{"id":"T16517","span":{"begin":1320,"end":1329},"obj":"VBD"},{"id":"T16518","span":{"begin":1330,"end":1332},"obj":"IN"},{"id":"T16519","span":{"begin":1333,"end":1336},"obj":"DT"},{"id":"T16520","span":{"begin":1367,"end":1377},"obj":"NNS"},{"id":"T16521","span":{"begin":1337,"end":1345},"obj":"NNP"},{"id":"T16522","span":{"begin":1346,"end":1356},"obj":"NNPS"},{"id":"T16523","span":{"begin":1357,"end":1359},"obj":"IN"},{"id":"T16524","span":{"begin":1360,"end":1366},"obj":"NNP"},{"id":"T16525","span":{"begin":1378,"end":1381},"obj":"IN"},{"id":"T16526","span":{"begin":1382,"end":1385},"obj":"NN"},{"id":"T16527","span":{"begin":1386,"end":1388},"obj":"IN"},{"id":"T16528","span":{"begin":1389,"end":1401},"obj":"JJ"},{"id":"T16529","span":{"begin":1402,"end":1409},"obj":"NNS"},{"id":"T16530","span":{"begin":1409,"end":1410},"obj":"."}],"relations":[{"id":"R4631","pred":"nsubj","subj":"T16209","obj":"T16210"},{"id":"R4632","pred":"compound","subj":"T16211","obj":"T16212"},{"id":"R4633","pred":"compound","subj":"T16212","obj":"T16213"},{"id":"R4634","pred":"dobj","subj":"T16213","obj":"T16210"},{"id":"R4635","pred":"prep","subj":"T16214","obj":"T16213"},{"id":"R4636","pred":"det","subj":"T16215","obj":"T16216"},{"id":"R4637","pred":"pobj","subj":"T16216","obj":"T16214"},{"id":"R4638","pred":"nmod","subj":"T16217","obj":"T16216"},{"id":"R4639","pred":"punct","subj":"T16218","obj":"T16217"},{"id":"R4640","pred":"nummod","subj":"T16219","obj":"T16217"},{"id":"R4641","pred":"punct","subj":"T16220","obj":"T16221"},{"id":"R4642","pred":"parataxis","subj":"T16221","obj":"T16213"},{"id":"R4643","pred":"compound","subj":"T16222","obj":"T16221"},{"id":"R4644","pred":"punct","subj":"T16223","obj":"T16221"},{"id":"R4645","pred":"compound","subj":"T16224","obj":"T16225"},{"id":"R4646","pred":"npadvmod","subj":"T16225","obj":"T16221"},{"id":"R4647","pred":"punct","subj":"T16226","obj":"T16221"},{"id":"R4648","pred":"npadvmod","subj":"T16227","obj":"T16221"},{"id":"R4649","pred":"punct","subj":"T16228","obj":"T16221"},{"id":"R4650","pred":"compound","subj":"T16229","obj":"T16230"},{"id":"R4651","pred":"npadvmod","subj":"T16230","obj":"T16221"},{"id":"R4652","pred":"punct","subj":"T16231","obj":"T16221"},{"id":"R4653","pred":"prep","subj":"T16232","obj":"T16210"},{"id":"R4654","pred":"nmod","subj":"T16233","obj":"T16234"},{"id":"R4655","pred":"compound","subj":"T16234","obj":"T16237"},{"id":"R4656","pred":"punct","subj":"T16235","obj":"T16233"},{"id":"R4657","pred":"nummod","subj":"T16236","obj":"T16233"},{"id":"R4658","pred":"pobj","subj":"T16237","obj":"T16232"},{"id":"R4659","pred":"prep","subj":"T16238","obj":"T16210"},{"id":"R4660","pred":"det","subj":"T16239","obj":"T16240"},{"id":"R4661","pred":"pobj","subj":"T16240","obj":"T16238"},{"id":"R4662","pred":"nummod","subj":"T16241","obj":"T16242"},{"id":"R4663","pred":"compound","subj":"T16242","obj":"T16240"},{"id":"R4664","pred":"aux","subj":"T16243","obj":"T16244"},{"id":"R4665","pred":"advcl","subj":"T16244","obj":"T16210"},{"id":"R4666","pred":"amod","subj":"T16245","obj":"T16246"},{"id":"R4667","pred":"compound","subj":"T16246","obj":"T16247"},{"id":"R4668","pred":"dobj","subj":"T16247","obj":"T16244"},{"id":"R4669","pred":"punct","subj":"T16248","obj":"T16210"},{"id":"R4670","pred":"nsubjpass","subj":"T16250","obj":"T16251"},{"id":"R4671","pred":"auxpass","subj":"T16252","obj":"T16251"},{"id":"R4672","pred":"prep","subj":"T16253","obj":"T16251"},{"id":"R4673","pred":"pobj","subj":"T16254","obj":"T16253"},{"id":"R4674","pred":"punct","subj":"T16255","obj":"T16251"},{"id":"R4675","pred":"cc","subj":"T16256","obj":"T16251"},{"id":"R4676","pred":"nsubjpass","subj":"T16257","obj":"T16258"},{"id":"R4677","pred":"conj","subj":"T16258","obj":"T16251"},{"id":"R4678","pred":"amod","subj":"T16259","obj":"T16257"},{"id":"R4679","pred":"prep","subj":"T16260","obj":"T16259"},{"id":"R4680","pred":"det","subj":"T16261","obj":"T16262"},{"id":"R4681","pred":"pobj","subj":"T16262","obj":"T16260"},{"id":"R4682","pred":"auxpass","subj":"T16263","obj":"T16258"},{"id":"R4683","pred":"aux","subj":"T16264","obj":"T16265"},{"id":"R4684","pred":"advcl","subj":"T16265","obj":"T16258"},{"id":"R4685","pred":"det","subj":"T16266","obj":"T16267"},{"id":"R4686","pred":"dobj","subj":"T16267","obj":"T16265"},{"id":"R4687","pred":"amod","subj":"T16268","obj":"T16267"},{"id":"R4688","pred":"punct","subj":"T16269","obj":"T16258"},{"id":"R4689","pred":"nsubj","subj":"T16271","obj":"T16272"},{"id":"R4690","pred":"acl","subj":"T16273","obj":"T16271"},{"id":"R4691","pred":"prep","subj":"T16274","obj":"T16273"},{"id":"R4692","pred":"det","subj":"T16275","obj":"T16276"},{"id":"R4693","pred":"pobj","subj":"T16276","obj":"T16274"},{"id":"R4694","pred":"compound","subj":"T16277","obj":"T16276"},{"id":"R4695","pred":"attr","subj":"T16278","obj":"T16272"},{"id":"R4696","pred":"punct","subj":"T16279","obj":"T16278"},{"id":"R4697","pred":"compound","subj":"T16280","obj":"T16281"},{"id":"R4698","pred":"appos","subj":"T16281","obj":"T16278"},{"id":"R4699","pred":"compound","subj":"T16282","obj":"T16281"},{"id":"R4700","pred":"compound","subj":"T16283","obj":"T16281"},{"id":"R4701","pred":"compound","subj":"T16284","obj":"T16281"},{"id":"R4702","pred":"compound","subj":"T16285","obj":"T16281"},{"id":"R4703","pred":"compound","subj":"T16286","obj":"T16281"},{"id":"R4704","pred":"punct","subj":"T16287","obj":"T16278"},{"id":"R4705","pred":"conj","subj":"T16288","obj":"T16278"},{"id":"R4706","pred":"punct","subj":"T16289","obj":"T16288"},{"id":"R4707","pred":"compound","subj":"T16290","obj":"T16291"},{"id":"R4708","pred":"appos","subj":"T16291","obj":"T16288"},{"id":"R4709","pred":"compound","subj":"T16292","obj":"T16291"},{"id":"R4710","pred":"compound","subj":"T16293","obj":"T16291"},{"id":"R4711","pred":"compound","subj":"T16294","obj":"T16291"},{"id":"R4712","pred":"compound","subj":"T16295","obj":"T16291"},{"id":"R4713","pred":"compound","subj":"T16296","obj":"T16291"},{"id":"R4714","pred":"punct","subj":"T16297","obj":"T16288"},{"id":"R4715","pred":"cc","subj":"T16298","obj":"T16288"},{"id":"R4716","pred":"conj","subj":"T16299","obj":"T16288"},{"id":"R4717","pred":"punct","subj":"T16300","obj":"T16299"},{"id":"R4718","pred":"compound","subj":"T16301","obj":"T16302"},{"id":"R4719","pred":"appos","subj":"T16302","obj":"T16299"},{"id":"R4720","pred":"compound","subj":"T16303","obj":"T16302"},{"id":"R4721","pred":"compound","subj":"T16304","obj":"T16302"},{"id":"R4722","pred":"compound","subj":"T16305","obj":"T16302"},{"id":"R4723","pred":"compound","subj":"T16306","obj":"T16302"},{"id":"R4724","pred":"compound","subj":"T16307","obj":"T16302"},{"id":"R4725","pred":"punct","subj":"T16308","obj":"T16272"},{"id":"R4726","pred":"nsubj","subj":"T16310","obj":"T16311"},{"id":"R4727","pred":"acl","subj":"T16312","obj":"T16310"},{"id":"R4728","pred":"prep","subj":"T16313","obj":"T16312"},{"id":"R4729","pred":"det","subj":"T16314","obj":"T16315"},{"id":"R4730","pred":"pobj","subj":"T16315","obj":"T16313"},{"id":"R4731","pred":"nmod","subj":"T16316","obj":"T16315"},{"id":"R4732","pred":"punct","subj":"T16317","obj":"T16316"},{"id":"R4733","pred":"nummod","subj":"T16318","obj":"T16316"},{"id":"R4734","pred":"attr","subj":"T16319","obj":"T16311"},{"id":"R4735","pred":"punct","subj":"T16320","obj":"T16319"},{"id":"R4736","pred":"compound","subj":"T16321","obj":"T16322"},{"id":"R4737","pred":"appos","subj":"T16322","obj":"T16319"},{"id":"R4738","pred":"compound","subj":"T16323","obj":"T16322"},{"id":"R4739","pred":"compound","subj":"T16324","obj":"T16322"},{"id":"R4740","pred":"compound","subj":"T16325","obj":"T16322"},{"id":"R4741","pred":"compound","subj":"T16326","obj":"T16322"},{"id":"R4742","pred":"compound","subj":"T16327","obj":"T16322"},{"id":"R4743","pred":"compound","subj":"T16328","obj":"T16322"},{"id":"R4744","pred":"punct","subj":"T16329","obj":"T16319"},{"id":"R4745","pred":"conj","subj":"T16330","obj":"T16319"},{"id":"R4746","pred":"punct","subj":"T16331","obj":"T16330"},{"id":"R4747","pred":"compound","subj":"T16332","obj":"T16333"},{"id":"R4748","pred":"appos","subj":"T16333","obj":"T16330"},{"id":"R4749","pred":"compound","subj":"T16334","obj":"T16333"},{"id":"R4750","pred":"compound","subj":"T16335","obj":"T16333"},{"id":"R4751","pred":"compound","subj":"T16336","obj":"T16333"},{"id":"R4752","pred":"compound","subj":"T16337","obj":"T16333"},{"id":"R4753","pred":"compound","subj":"T16338","obj":"T16333"},{"id":"R4754","pred":"compound","subj":"T16339","obj":"T16333"},{"id":"R4755","pred":"punct","subj":"T16340","obj":"T16330"},{"id":"R4756","pred":"conj","subj":"T16341","obj":"T16330"},{"id":"R4757","pred":"punct","subj":"T16342","obj":"T16341"},{"id":"R4758","pred":"compound","subj":"T16343","obj":"T16344"},{"id":"R4759","pred":"appos","subj":"T16344","obj":"T16341"},{"id":"R4760","pred":"compound","subj":"T16345","obj":"T16344"},{"id":"R4761","pred":"compound","subj":"T16346","obj":"T16344"},{"id":"R4762","pred":"compound","subj":"T16347","obj":"T16344"},{"id":"R4763","pred":"compound","subj":"T16348","obj":"T16344"},{"id":"R4764","pred":"compound","subj":"T16349","obj":"T16344"},{"id":"R4765","pred":"punct","subj":"T16350","obj":"T16341"},{"id":"R4766","pred":"cc","subj":"T16351","obj":"T16341"},{"id":"R4767","pred":"conj","subj":"T16352","obj":"T16341"},{"id":"R4768","pred":"punct","subj":"T16353","obj":"T16352"},{"id":"R4769","pred":"compound","subj":"T16354","obj":"T16355"},{"id":"R4770","pred":"appos","subj":"T16355","obj":"T16352"},{"id":"R4771","pred":"compound","subj":"T16356","obj":"T16355"},{"id":"R4772","pred":"compound","subj":"T16357","obj":"T16355"},{"id":"R4773","pred":"compound","subj":"T16358","obj":"T16355"},{"id":"R4774","pred":"compound","subj":"T16359","obj":"T16355"},{"id":"R4775","pred":"punct","subj":"T16360","obj":"T16311"},{"id":"R4776","pred":"nmod","subj":"T16362","obj":"T16363"},{"id":"R4777","pred":"nsubjpass","subj":"T16363","obj":"T16373"},{"id":"R4778","pred":"punct","subj":"T16364","obj":"T16362"},{"id":"R4779","pred":"compound","subj":"T16365","obj":"T16366"},{"id":"R4780","pred":"conj","subj":"T16366","obj":"T16362"},{"id":"R4781","pred":"punct","subj":"T16367","obj":"T16366"},{"id":"R4782","pred":"cc","subj":"T16368","obj":"T16366"},{"id":"R4783","pred":"nmod","subj":"T16369","obj":"T16370"},{"id":"R4784","pred":"conj","subj":"T16370","obj":"T16366"},{"id":"R4785","pred":"punct","subj":"T16371","obj":"T16369"},{"id":"R4786","pred":"nummod","subj":"T16372","obj":"T16369"},{"id":"R4787","pred":"auxpass","subj":"T16374","obj":"T16373"},{"id":"R4788","pred":"prep","subj":"T16375","obj":"T16373"},{"id":"R4789","pred":"pobj","subj":"T16376","obj":"T16375"},{"id":"R4790","pred":"punct","subj":"T16377","obj":"T16373"},{"id":"R4791","pred":"det","subj":"T16379","obj":"T16380"},{"id":"R4792","pred":"nsubjpass","subj":"T16380","obj":"T16381"},{"id":"R4793","pred":"ccomp","subj":"T16381","obj":"T16393"},{"id":"R4794","pred":"prep","subj":"T16382","obj":"T16380"},{"id":"R4795","pred":"nummod","subj":"T16383","obj":"T16384"},{"id":"R4796","pred":"pobj","subj":"T16384","obj":"T16382"},{"id":"R4797","pred":"nmod","subj":"T16385","obj":"T16386"},{"id":"R4798","pred":"compound","subj":"T16386","obj":"T16384"},{"id":"R4799","pred":"punct","subj":"T16387","obj":"T16385"},{"id":"R4800","pred":"appos","subj":"T16388","obj":"T16385"},{"id":"R4801","pred":"punct","subj":"T16389","obj":"T16388"},{"id":"R4802","pred":"nummod","subj":"T16390","obj":"T16388"},{"id":"R4803","pred":"amod","subj":"T16391","obj":"T16386"},{"id":"R4804","pred":"auxpass","subj":"T16392","obj":"T16381"},{"id":"R4805","pred":"punct","subj":"T16394","obj":"T16393"},{"id":"R4806","pred":"prep","subj":"T16395","obj":"T16393"},{"id":"R4807","pred":"pobj","subj":"T16396","obj":"T16395"},{"id":"R4808","pred":"nsubj","subj":"T16397","obj":"T16393"},{"id":"R4809","pred":"compound","subj":"T16398","obj":"T16399"},{"id":"R4810","pred":"attr","subj":"T16399","obj":"T16393"},{"id":"R4811","pred":"punct","subj":"T16400","obj":"T16393"},{"id":"R4812","pred":"det","subj":"T16402","obj":"T16403"},{"id":"R4813","pred":"nsubjpass","subj":"T16403","obj":"T16405"},{"id":"R4814","pred":"compound","subj":"T16404","obj":"T16403"},{"id":"R4815","pred":"prep","subj":"T16406","obj":"T16403"},{"id":"R4816","pred":"det","subj":"T16407","obj":"T16408"},{"id":"R4817","pred":"pobj","subj":"T16408","obj":"T16406"},{"id":"R4818","pred":"nummod","subj":"T16409","obj":"T16410"},{"id":"R4819","pred":"compound","subj":"T16410","obj":"T16408"},{"id":"R4820","pred":"auxpass","subj":"T16411","obj":"T16405"},{"id":"R4821","pred":"prep","subj":"T16412","obj":"T16405"},{"id":"R4822","pred":"compound","subj":"T16413","obj":"T16414"},{"id":"R4823","pred":"pobj","subj":"T16414","obj":"T16412"},{"id":"R4824","pred":"punct","subj":"T16415","obj":"T16405"},{"id":"R4825","pred":"nsubj","subj":"T16417","obj":"T16418"},{"id":"R4826","pred":"compound","subj":"T16419","obj":"T16420"},{"id":"R4827","pred":"dobj","subj":"T16420","obj":"T16418"},{"id":"R4828","pred":"aux","subj":"T16421","obj":"T16422"},{"id":"R4829","pred":"advcl","subj":"T16422","obj":"T16418"},{"id":"R4830","pred":"dobj","subj":"T16423","obj":"T16422"},{"id":"R4831","pred":"advcl","subj":"T16424","obj":"T16422"},{"id":"R4832","pred":"det","subj":"T16425","obj":"T16426"},{"id":"R4833","pred":"dobj","subj":"T16426","obj":"T16424"},{"id":"R4834","pred":"appos","subj":"T16427","obj":"T16426"},{"id":"R4835","pred":"punct","subj":"T16428","obj":"T16427"},{"id":"R4836","pred":"compound","subj":"T16429","obj":"T16430"},{"id":"R4837","pred":"appos","subj":"T16430","obj":"T16427"},{"id":"R4838","pred":"compound","subj":"T16431","obj":"T16430"},{"id":"R4839","pred":"compound","subj":"T16432","obj":"T16430"},{"id":"R4840","pred":"compound","subj":"T16433","obj":"T16430"},{"id":"R4841","pred":"compound","subj":"T16434","obj":"T16430"},{"id":"R4842","pred":"compound","subj":"T16435","obj":"T16430"},{"id":"R4843","pred":"punct","subj":"T16436","obj":"T16427"},{"id":"R4844","pred":"conj","subj":"T16437","obj":"T16427"},{"id":"R4845","pred":"punct","subj":"T16438","obj":"T16437"},{"id":"R4846","pred":"compound","subj":"T16439","obj":"T16440"},{"id":"R4847","pred":"appos","subj":"T16440","obj":"T16437"},{"id":"R4848","pred":"compound","subj":"T16441","obj":"T16440"},{"id":"R4849","pred":"compound","subj":"T16442","obj":"T16440"},{"id":"R4850","pred":"compound","subj":"T16443","obj":"T16440"},{"id":"R4851","pred":"compound","subj":"T16444","obj":"T16440"},{"id":"R4852","pred":"compound","subj":"T16445","obj":"T16440"},{"id":"R4853","pred":"punct","subj":"T16446","obj":"T16437"},{"id":"R4854","pred":"amod","subj":"T16447","obj":"T16448"},{"id":"R4855","pred":"appos","subj":"T16448","obj":"T16437"},{"id":"R4856","pred":"compound","subj":"T16449","obj":"T16448"},{"id":"R4857","pred":"punct","subj":"T16450","obj":"T16437"},{"id":"R4858","pred":"punct","subj":"T16451","obj":"T16437"},{"id":"R4859","pred":"conj","subj":"T16452","obj":"T16437"},{"id":"R4860","pred":"punct","subj":"T16453","obj":"T16452"},{"id":"R4861","pred":"compound","subj":"T16454","obj":"T16455"},{"id":"R4862","pred":"appos","subj":"T16455","obj":"T16452"},{"id":"R4863","pred":"compound","subj":"T16456","obj":"T16455"},{"id":"R4864","pred":"compound","subj":"T16457","obj":"T16455"},{"id":"R4865","pred":"compound","subj":"T16458","obj":"T16455"},{"id":"R4866","pred":"compound","subj":"T16459","obj":"T16455"},{"id":"R4867","pred":"compound","subj":"T16460","obj":"T16455"},{"id":"R4868","pred":"compound","subj":"T16461","obj":"T16455"},{"id":"R4869","pred":"punct","subj":"T16462","obj":"T16452"},{"id":"R4870","pred":"cc","subj":"T16463","obj":"T16452"},{"id":"R4871","pred":"conj","subj":"T16464","obj":"T16452"},{"id":"R4872","pred":"punct","subj":"T16465","obj":"T16464"},{"id":"R4873","pred":"compound","subj":"T16466","obj":"T16467"},{"id":"R4874","pred":"appos","subj":"T16467","obj":"T16464"},{"id":"R4875","pred":"compound","subj":"T16468","obj":"T16467"},{"id":"R4876","pred":"compound","subj":"T16469","obj":"T16467"},{"id":"R4877","pred":"compound","subj":"T16470","obj":"T16467"},{"id":"R4878","pred":"compound","subj":"T16471","obj":"T16467"},{"id":"R4879","pred":"compound","subj":"T16472","obj":"T16467"},{"id":"R4880","pred":"punct","subj":"T16473","obj":"T16474"},{"id":"R4881","pred":"appos","subj":"T16474","obj":"T16464"},{"id":"R4882","pred":"compound","subj":"T16475","obj":"T16474"},{"id":"R4883","pred":"punct","subj":"T16476","obj":"T16464"},{"id":"R4884","pred":"punct","subj":"T16477","obj":"T16418"},{"id":"R4885","pred":"prep","subj":"T16479","obj":"T16480"},{"id":"R4886","pred":"amod","subj":"T16481","obj":"T16482"},{"id":"R4887","pred":"pobj","subj":"T16482","obj":"T16479"},{"id":"R4888","pred":"punct","subj":"T16483","obj":"T16480"},{"id":"R4889","pred":"nsubjpass","subj":"T16484","obj":"T16480"},{"id":"R4890","pred":"prep","subj":"T16485","obj":"T16484"},{"id":"R4891","pred":"compound","subj":"T16486","obj":"T16487"},{"id":"R4892","pred":"pobj","subj":"T16487","obj":"T16485"},{"id":"R4893","pred":"auxpass","subj":"T16488","obj":"T16480"},{"id":"R4894","pred":"oprd","subj":"T16489","obj":"T16480"},{"id":"R4895","pred":"punct","subj":"T16490","obj":"T16480"},{"id":"R4896","pred":"nsubjpass","subj":"T16492","obj":"T16493"},{"id":"R4897","pred":"prep","subj":"T16494","obj":"T16492"},{"id":"R4898","pred":"det","subj":"T16495","obj":"T16496"},{"id":"R4899","pred":"pobj","subj":"T16496","obj":"T16494"},{"id":"R4900","pred":"acl","subj":"T16497","obj":"T16496"},{"id":"R4901","pred":"prep","subj":"T16498","obj":"T16497"},{"id":"R4902","pred":"det","subj":"T16499","obj":"T16500"},{"id":"R4903","pred":"pobj","subj":"T16500","obj":"T16498"},{"id":"R4904","pred":"auxpass","subj":"T16501","obj":"T16493"},{"id":"R4905","pred":"agent","subj":"T16502","obj":"T16493"},{"id":"R4906","pred":"det","subj":"T16503","obj":"T16504"},{"id":"R4907","pred":"pobj","subj":"T16504","obj":"T16502"},{"id":"R4908","pred":"nmod","subj":"T16505","obj":"T16506"},{"id":"R4909","pred":"nmod","subj":"T16506","obj":"T16508"},{"id":"R4910","pred":"nmod","subj":"T16507","obj":"T16506"},{"id":"R4911","pred":"nmod","subj":"T16508","obj":"T16504"},{"id":"R4912","pred":"nmod","subj":"T16509","obj":"T16510"},{"id":"R4913","pred":"nmod","subj":"T16510","obj":"T16508"},{"id":"R4914","pred":"nmod","subj":"T16511","obj":"T16512"},{"id":"R4915","pred":"nmod","subj":"T16512","obj":"T16513"},{"id":"R4916","pred":"nmod","subj":"T16513","obj":"T16504"},{"id":"R4917","pred":"cc","subj":"T16514","obj":"T16513"},{"id":"R4918","pred":"conj","subj":"T16515","obj":"T16513"},{"id":"R4919","pred":"cc","subj":"T16516","obj":"T16493"},{"id":"R4920","pred":"conj","subj":"T16517","obj":"T16493"},{"id":"R4921","pred":"prep","subj":"T16518","obj":"T16517"},{"id":"R4922","pred":"det","subj":"T16519","obj":"T16520"},{"id":"R4923","pred":"pobj","subj":"T16520","obj":"T16518"},{"id":"R4924","pred":"nmod","subj":"T16521","obj":"T16522"},{"id":"R4925","pred":"nmod","subj":"T16522","obj":"T16520"},{"id":"R4926","pred":"prep","subj":"T16523","obj":"T16522"},{"id":"R4927","pred":"pobj","subj":"T16524","obj":"T16523"},{"id":"R4928","pred":"prep","subj":"T16525","obj":"T16520"},{"id":"R4929","pred":"pobj","subj":"T16526","obj":"T16525"},{"id":"R4930","pred":"prep","subj":"T16527","obj":"T16526"},{"id":"R4931","pred":"amod","subj":"T16528","obj":"T16529"},{"id":"R4932","pred":"pobj","subj":"T16529","obj":"T16527"},{"id":"R4933","pred":"punct","subj":"T16530","obj":"T16493"}],"text":"We crossed Bax KO females on a C57BL/6 background (Jackson Laboratory, Bar Harbor, Maine, United States) to NT-3 heterozygote males on a 129 Sv background to generate double heterozygote animals. Progeny was genotyped with PCR, and animals heterozygous for both genes were bred to obtain the double KOs. Primers used for the Bax locus were R661, GTT GAC CAG AGT GGC GTA GG; R662, CCG CTT CCA TTG CTC AGC GG; and R663, GAG CTG ATC AGA ACC ATC ATG. Primers used for the NT-3 locus were NT3A, CGT GGT GAG GTT CTA TTG GCT AC; NT3B, CAG AGC ACC CTG CCC AAA GCA GAG; NT3R, CCT TGA CAA TAC TGA ATG CC; and NEOF, GGG AAC TTC CTG ACT AGG. WT, Bax KO, and NT-3 KO littermates were used as controls. A total of 11 Bax/NT-3 double KO mice were analyzed, of these nine were P0 pups. The p75 colony on a 129 S1 background was received from Jackson Laboratory. We used tail DNA to genotype animals using the primers IMR0013, CTT GGG TGG AGA GGC TAT TC; IMR0014, AGG TGA GAT GAC AGG AGA TC (generic neo primers); IMR0710, TGT TAC GTT CTC TGA CGT GGT GAG; and IMR0711, TCA GCC CAG GGT GTG CAC TC (p75 locus). For embryonic experiments, day of plug positivity was considered E0. All of the protocols used in this study were approved by the Louisiana State University Health Sciences Center Institutional Animal Care and Use Committee and conformed to the National Institutes of Health guidelines for use of experimental animals."}