| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T12726 |
333-334 |
NN |
denotes |
% |
| T12657 |
0-5 |
NNS |
denotes |
Limbs |
| T12658 |
62-71 |
VBN |
denotes |
dissected |
| T12659 |
6-10 |
IN |
denotes |
from |
| T12660 |
11-17 |
JJ |
denotes |
mutant |
| T12661 |
30-37 |
NNS |
denotes |
animals |
| T12662 |
18-21 |
CC |
denotes |
and |
| T12663 |
22-29 |
NN |
denotes |
control |
| T12664 |
38-40 |
IN |
denotes |
at |
| T12665 |
41-46 |
NN |
denotes |
E13.5 |
| T12666 |
47-50 |
CC |
denotes |
and |
| T12667 |
51-56 |
NN |
denotes |
E14.5 |
| T12668 |
57-61 |
VBD |
denotes |
were |
| T12669 |
72-75 |
CC |
denotes |
and |
| T12670 |
76-82 |
VBN |
denotes |
frozen |
| T12671 |
83-85 |
IN |
denotes |
in |
| T12672 |
86-89 |
NN |
denotes |
OCT |
| T12673 |
90-91 |
-LRB- |
denotes |
( |
| T12674 |
98-105 |
NNP |
denotes |
Finetek |
| T12675 |
91-97 |
NNP |
denotes |
Sakura |
| T12676 |
105-106 |
, |
denotes |
, |
| T12677 |
106-114 |
NNP |
denotes |
Torrence |
| T12678 |
114-116 |
, |
denotes |
, |
| T12679 |
116-118 |
NNP |
denotes |
CA |
| T12680 |
118-120 |
, |
denotes |
, |
| T12681 |
120-126 |
NNP |
denotes |
United |
| T12682 |
127-133 |
NNP |
denotes |
States |
| T12683 |
133-134 |
-RRB- |
denotes |
) |
| T12684 |
134-135 |
. |
denotes |
. |
| T12685 |
135-265 |
sentence |
denotes |
Cryosections of tissue were assayed by TUNEL using the In Situ Cell Death Detection Kit, Fluorescein (Roche, Basel, Switzerland). |
| T12686 |
136-148 |
NNS |
denotes |
Cryosections |
| T12687 |
164-171 |
VBN |
denotes |
assayed |
| T12688 |
149-151 |
IN |
denotes |
of |
| T12689 |
152-158 |
NN |
denotes |
tissue |
| T12690 |
159-163 |
VBD |
denotes |
were |
| T12691 |
172-174 |
IN |
denotes |
by |
| T12692 |
175-180 |
NN |
denotes |
TUNEL |
| T12693 |
181-186 |
VBG |
denotes |
using |
| T12694 |
187-190 |
DT |
denotes |
the |
| T12695 |
220-223 |
NN |
denotes |
Kit |
| T12696 |
191-193 |
FW |
denotes |
In |
| T12697 |
194-198 |
FW |
denotes |
Situ |
| T12698 |
210-219 |
NN |
denotes |
Detection |
| T12699 |
199-203 |
NN |
denotes |
Cell |
| T12700 |
204-209 |
NN |
denotes |
Death |
| T12701 |
223-225 |
, |
denotes |
, |
| T12702 |
225-236 |
NNP |
denotes |
Fluorescein |
| T12703 |
237-238 |
-LRB- |
denotes |
( |
| T12704 |
238-243 |
NNP |
denotes |
Roche |
| T12705 |
243-245 |
, |
denotes |
, |
| T12706 |
245-250 |
NNP |
denotes |
Basel |
| T12707 |
250-252 |
, |
denotes |
, |
| T12708 |
252-263 |
NNP |
denotes |
Switzerland |
| T12709 |
263-264 |
-RRB- |
denotes |
) |
| T12710 |
264-265 |
. |
denotes |
. |
| T12711 |
265-569 |
sentence |
denotes |
Following TUNEL, slides were washed in PBS, blocked with PBS + 0.05% Tween-20 + 5% goat serum, washed again, and incubated with a 1:200 dilution of a rabbit anti-phospho-histone-H3 antibody called Mitosis Marker (Upstate Biotechnology, Lake Placid, New York, United States) to identify cells in mitosis. |
| T12712 |
266-275 |
VBG |
denotes |
Following |
| T12713 |
295-301 |
VBN |
denotes |
washed |
| T12714 |
276-281 |
NN |
denotes |
TUNEL |
| T12715 |
281-283 |
, |
denotes |
, |
| T12716 |
283-289 |
NNS |
denotes |
slides |
| T12717 |
290-294 |
VBD |
denotes |
were |
| T12718 |
302-304 |
IN |
denotes |
in |
| T12719 |
305-308 |
NN |
denotes |
PBS |
| T12720 |
308-310 |
, |
denotes |
, |
| T12721 |
310-317 |
VBN |
denotes |
blocked |
| T12722 |
318-322 |
IN |
denotes |
with |
| T12723 |
323-326 |
NN |
denotes |
PBS |
| T12724 |
327-328 |
SYM |
denotes |
+ |
| T12725 |
329-333 |
CD |
denotes |
0.05 |
| T12727 |
335-340 |
NN |
denotes |
Tween |
| T12728 |
340-341 |
HYPH |
denotes |
- |
| T12729 |
341-343 |
CD |
denotes |
20 |
| T12730 |
344-345 |
SYM |
denotes |
+ |
| T12731 |
346-347 |
CD |
denotes |
5 |
| T12732 |
347-348 |
NN |
denotes |
% |
| T12733 |
354-359 |
NN |
denotes |
serum |
| T12734 |
349-353 |
NN |
denotes |
goat |
| T12735 |
359-361 |
, |
denotes |
, |
| T12736 |
361-367 |
VBD |
denotes |
washed |
| T12737 |
368-373 |
RB |
denotes |
again |
| T12738 |
373-375 |
, |
denotes |
, |
| T12739 |
375-378 |
CC |
denotes |
and |
| T12740 |
379-388 |
VBN |
denotes |
incubated |
| T12741 |
389-393 |
IN |
denotes |
with |
| T12742 |
394-395 |
DT |
denotes |
a |
| T12743 |
402-410 |
NN |
denotes |
dilution |
| T12744 |
396-397 |
CD |
denotes |
1 |
| T12745 |
397-398 |
SYM |
denotes |
: |
| T12746 |
398-401 |
CD |
denotes |
200 |
| T12747 |
411-413 |
IN |
denotes |
of |
| T12748 |
414-415 |
DT |
denotes |
a |
| T12749 |
447-455 |
NN |
denotes |
antibody |
| T12750 |
416-422 |
NN |
denotes |
rabbit |
| T12751 |
423-443 |
JJ |
denotes |
anti-phospho-histone |
| T12752 |
444-446 |
NN |
denotes |
H3 |
| T12753 |
443-444 |
HYPH |
denotes |
- |
| T12754 |
456-462 |
VBN |
denotes |
called |
| T12755 |
463-470 |
NN |
denotes |
Mitosis |
| T12756 |
471-477 |
NN |
denotes |
Marker |
| T12757 |
478-479 |
-LRB- |
denotes |
( |
| T12758 |
487-500 |
NNP |
denotes |
Biotechnology |
| T12759 |
479-486 |
NNP |
denotes |
Upstate |
| T12760 |
500-502 |
, |
denotes |
, |
| T12761 |
502-506 |
NNP |
denotes |
Lake |
| T12762 |
507-513 |
NNP |
denotes |
Placid |
| T12763 |
513-515 |
, |
denotes |
, |
| T12764 |
515-518 |
NNP |
denotes |
New |
| T12765 |
519-523 |
NNP |
denotes |
York |
| T12766 |
523-525 |
, |
denotes |
, |
| T12767 |
525-531 |
NNP |
denotes |
United |
| T12768 |
532-538 |
NNP |
denotes |
States |
| T12769 |
538-539 |
-RRB- |
denotes |
) |
| T12770 |
540-542 |
TO |
denotes |
to |
| T12771 |
543-551 |
VB |
denotes |
identify |
| T12772 |
552-557 |
NNS |
denotes |
cells |
| T12773 |
558-560 |
IN |
denotes |
in |
| T12774 |
561-568 |
NN |
denotes |
mitosis |
| T12775 |
568-569 |
. |
denotes |
. |
| T12776 |
569-645 |
sentence |
denotes |
Cy3-labeled anti-rabbit secondary antibody was used to detect the antibody. |
| T12777 |
570-573 |
NN |
denotes |
Cy3 |
| T12778 |
574-581 |
VBN |
denotes |
labeled |
| T12779 |
573-574 |
HYPH |
denotes |
- |
| T12780 |
604-612 |
NN |
denotes |
antibody |
| T12781 |
582-593 |
JJ |
denotes |
anti-rabbit |
| T12782 |
594-603 |
JJ |
denotes |
secondary |
| T12783 |
617-621 |
VBN |
denotes |
used |
| T12784 |
613-616 |
VBD |
denotes |
was |
| T12785 |
622-624 |
TO |
denotes |
to |
| T12786 |
625-631 |
VB |
denotes |
detect |
| T12787 |
632-635 |
DT |
denotes |
the |
| T12788 |
636-644 |
NN |
denotes |
antibody |
| T12789 |
644-645 |
. |
denotes |
. |
| T12790 |
645-818 |
sentence |
denotes |
Cell nuclei were labeled with DAPI, and slides were mounted in Vectamount (Vector Laboratories, Burlingame, California, United States) and visualized at 100× magnification. |
| T12791 |
646-650 |
NN |
denotes |
Cell |
| T12792 |
651-657 |
NNS |
denotes |
nuclei |
| T12793 |
663-670 |
VBN |
denotes |
labeled |
| T12794 |
658-662 |
VBD |
denotes |
were |
| T12795 |
671-675 |
IN |
denotes |
with |
| T12796 |
676-680 |
NN |
denotes |
DAPI |
| T12797 |
680-682 |
, |
denotes |
, |
| T12798 |
682-685 |
CC |
denotes |
and |
| T12799 |
686-692 |
NNS |
denotes |
slides |
| T12800 |
698-705 |
VBN |
denotes |
mounted |
| T12801 |
693-697 |
VBD |
denotes |
were |
| T12802 |
706-708 |
IN |
denotes |
in |
| T12803 |
709-719 |
NNP |
denotes |
Vectamount |
| T12804 |
720-721 |
-LRB- |
denotes |
( |
| T12805 |
728-740 |
NNP |
denotes |
Laboratories |
| T12806 |
721-727 |
NNP |
denotes |
Vector |
| T12807 |
740-742 |
, |
denotes |
, |
| T12808 |
742-752 |
NNP |
denotes |
Burlingame |
| T12809 |
752-754 |
, |
denotes |
, |
| T12810 |
754-764 |
NNP |
denotes |
California |
| T12811 |
764-766 |
, |
denotes |
, |
| T12812 |
766-772 |
NNP |
denotes |
United |
| T12813 |
773-779 |
NNP |
denotes |
States |
| T12814 |
779-780 |
-RRB- |
denotes |
) |
| T12815 |
781-784 |
CC |
denotes |
and |
| T12816 |
785-795 |
VBN |
denotes |
visualized |
| T12817 |
796-798 |
IN |
denotes |
at |
| T12818 |
799-802 |
CD |
denotes |
100 |
| T12819 |
804-817 |
NN |
denotes |
magnification |
| T12820 |
802-803 |
SYM |
denotes |
× |
| T12821 |
817-818 |
. |
denotes |
. |
| T12822 |
818-1058 |
sentence |
denotes |
The area of selected anatomical sites were measured, and the number of TUNEL-labeled nuclear fragments and the number of Cy3-labeled nuclei were counted from three 10-μm sections spanning 50 μm, from three control and three mutant animals. |
| T12823 |
819-822 |
DT |
denotes |
The |
| T12824 |
823-827 |
NN |
denotes |
area |
| T12825 |
862-870 |
VBN |
denotes |
measured |
| T12826 |
828-830 |
IN |
denotes |
of |
| T12827 |
831-839 |
VBN |
denotes |
selected |
| T12828 |
851-856 |
NNS |
denotes |
sites |
| T12829 |
840-850 |
JJ |
denotes |
anatomical |
| T12830 |
857-861 |
VBD |
denotes |
were |
| T12831 |
870-872 |
, |
denotes |
, |
| T12832 |
872-875 |
CC |
denotes |
and |
| T12833 |
876-879 |
DT |
denotes |
the |
| T12834 |
880-886 |
NN |
denotes |
number |
| T12835 |
964-971 |
VBN |
denotes |
counted |
| T12836 |
887-889 |
IN |
denotes |
of |
| T12837 |
890-895 |
NN |
denotes |
TUNEL |
| T12838 |
896-903 |
VBN |
denotes |
labeled |
| T12839 |
895-896 |
HYPH |
denotes |
- |
| T12840 |
912-921 |
NNS |
denotes |
fragments |
| T12841 |
904-911 |
JJ |
denotes |
nuclear |
| T12842 |
922-925 |
CC |
denotes |
and |
| T12843 |
926-929 |
DT |
denotes |
the |
| T12844 |
930-936 |
NN |
denotes |
number |
| T12845 |
937-939 |
IN |
denotes |
of |
| T12846 |
940-943 |
NN |
denotes |
Cy3 |
| T12847 |
944-951 |
VBN |
denotes |
labeled |
| T12848 |
943-944 |
HYPH |
denotes |
- |
| T12849 |
952-958 |
NNS |
denotes |
nuclei |
| T12850 |
959-963 |
VBD |
denotes |
were |
| T12851 |
972-976 |
IN |
denotes |
from |
| T12852 |
977-982 |
CD |
denotes |
three |
| T12853 |
989-997 |
NNS |
denotes |
sections |
| T12854 |
983-985 |
CD |
denotes |
10 |
| T12855 |
986-988 |
NN |
denotes |
μm |
| T12856 |
985-986 |
HYPH |
denotes |
- |
| T12857 |
998-1006 |
VBG |
denotes |
spanning |
| T12858 |
1007-1009 |
CD |
denotes |
50 |
| T12859 |
1010-1012 |
NN |
denotes |
μm |
| T12860 |
1012-1014 |
, |
denotes |
, |
| T12861 |
1014-1018 |
IN |
denotes |
from |
| T12862 |
1019-1024 |
CD |
denotes |
three |
| T12863 |
1025-1032 |
NN |
denotes |
control |
| T12864 |
1033-1036 |
CC |
denotes |
and |
| T12865 |
1037-1042 |
CD |
denotes |
three |
| T12866 |
1043-1049 |
NN |
denotes |
mutant |
| T12867 |
1050-1057 |
NNS |
denotes |
animals |
| T12868 |
1057-1058 |
. |
denotes |
. |
| T12869 |
1058-1230 |
sentence |
denotes |
The number of labeled cells in the metacarpal-phalangeal and metatarsal-phalangeal joints was counted in a 290 μm × 365 μm rectangle placed around the center of the joint. |
| T12870 |
1059-1062 |
DT |
denotes |
The |
| T12871 |
1063-1069 |
NN |
denotes |
number |
| T12872 |
1153-1160 |
VBN |
denotes |
counted |
| T12873 |
1070-1072 |
IN |
denotes |
of |
| T12874 |
1073-1080 |
VBN |
denotes |
labeled |
| T12875 |
1081-1086 |
NNS |
denotes |
cells |
| T12876 |
1087-1089 |
IN |
denotes |
in |
| T12877 |
1090-1093 |
DT |
denotes |
the |
| T12878 |
1142-1148 |
NNS |
denotes |
joints |
| T12879 |
1094-1104 |
JJ |
denotes |
metacarpal |
| T12880 |
1105-1115 |
JJ |
denotes |
phalangeal |
| T12881 |
1104-1105 |
HYPH |
denotes |
- |
| T12882 |
1116-1119 |
CC |
denotes |
and |
| T12883 |
1120-1130 |
JJ |
denotes |
metatarsal |
| T12884 |
1131-1141 |
JJ |
denotes |
phalangeal |
| T12885 |
1130-1131 |
HYPH |
denotes |
- |
| T12886 |
1149-1152 |
VBD |
denotes |
was |
| T12887 |
1161-1163 |
IN |
denotes |
in |
| T12888 |
1164-1165 |
DT |
denotes |
a |
| T12889 |
1182-1191 |
NN |
denotes |
rectangle |
| T12890 |
1166-1169 |
CD |
denotes |
290 |
| T12891 |
1170-1172 |
NN |
denotes |
μm |
| T12892 |
1173-1174 |
SYM |
denotes |
× |
| T12893 |
1179-1181 |
NN |
denotes |
μm |
| T12894 |
1175-1178 |
CD |
denotes |
365 |
| T12895 |
1192-1198 |
VBN |
denotes |
placed |
| T12896 |
1199-1205 |
IN |
denotes |
around |
| T12897 |
1206-1209 |
DT |
denotes |
the |
| T12898 |
1210-1216 |
NN |
denotes |
center |
| T12899 |
1217-1219 |
IN |
denotes |
of |
| T12900 |
1220-1223 |
DT |
denotes |
the |
| T12901 |
1224-1229 |
NN |
denotes |
joint |
| T12902 |
1229-1230 |
. |
denotes |
. |
| T12903 |
1230-1385 |
sentence |
denotes |
The posterior region of the fifth digit was defined by drawing a line from the tip of the digit down 2.15 mm and across to the lateral edge of the tissue. |
| T12904 |
1231-1234 |
DT |
denotes |
The |
| T12905 |
1245-1251 |
NN |
denotes |
region |
| T12906 |
1235-1244 |
JJ |
denotes |
posterior |
| T12907 |
1275-1282 |
VBN |
denotes |
defined |
| T12908 |
1252-1254 |
IN |
denotes |
of |
| T12909 |
1255-1258 |
DT |
denotes |
the |
| T12910 |
1265-1270 |
NN |
denotes |
digit |
| T12911 |
1259-1264 |
JJ |
denotes |
fifth |
| T12912 |
1271-1274 |
VBD |
denotes |
was |
| T12913 |
1283-1285 |
IN |
denotes |
by |
| T12914 |
1286-1293 |
VBG |
denotes |
drawing |
| T12915 |
1294-1295 |
DT |
denotes |
a |
| T12916 |
1296-1300 |
NN |
denotes |
line |
| T12917 |
1301-1305 |
IN |
denotes |
from |
| T12918 |
1306-1309 |
DT |
denotes |
the |
| T12919 |
1310-1313 |
NN |
denotes |
tip |
| T12920 |
1314-1316 |
IN |
denotes |
of |
| T12921 |
1317-1320 |
DT |
denotes |
the |
| T12922 |
1321-1326 |
NN |
denotes |
digit |
| T12923 |
1327-1331 |
IN |
denotes |
down |
| T12924 |
1332-1336 |
CD |
denotes |
2.15 |
| T12925 |
1337-1339 |
NN |
denotes |
mm |
| T12926 |
1340-1343 |
CC |
denotes |
and |
| T12927 |
1344-1350 |
IN |
denotes |
across |
| T12928 |
1351-1353 |
IN |
denotes |
to |
| T12929 |
1354-1357 |
DT |
denotes |
the |
| T12930 |
1366-1370 |
NN |
denotes |
edge |
| T12931 |
1358-1365 |
JJ |
denotes |
lateral |
| T12932 |
1371-1373 |
IN |
denotes |
of |
| T12933 |
1374-1377 |
DT |
denotes |
the |
| T12934 |
1378-1384 |
NN |
denotes |
tissue |
| T12935 |
1384-1385 |
. |
denotes |
. |
| T12936 |
1385-1443 |
sentence |
denotes |
For this analysis, the R26R Cre reporter was not present. |
| T12937 |
1386-1389 |
IN |
denotes |
For |
| T12938 |
1427-1430 |
VBD |
denotes |
was |
| T12939 |
1390-1394 |
DT |
denotes |
this |
| T12940 |
1395-1403 |
NN |
denotes |
analysis |
| T12941 |
1403-1405 |
, |
denotes |
, |
| T12942 |
1405-1408 |
DT |
denotes |
the |
| T12943 |
1418-1426 |
NN |
denotes |
reporter |
| T12944 |
1409-1413 |
NN |
denotes |
R26R |
| T12945 |
1414-1417 |
NN |
denotes |
Cre |
| T12946 |
1431-1434 |
RB |
denotes |
not |
| T12947 |
1435-1442 |
JJ |
denotes |
present |
| T12948 |
1442-1443 |
. |
denotes |
. |
| R8323 |
T12657 |
T12658 |
nsubjpass |
Limbs,dissected |
| R8324 |
T12659 |
T12657 |
prep |
from,Limbs |
| R8325 |
T12660 |
T12661 |
amod |
mutant,animals |
| R8326 |
T12661 |
T12659 |
pobj |
animals,from |
| R8327 |
T12662 |
T12660 |
cc |
and,mutant |
| R8328 |
T12663 |
T12660 |
conj |
control,mutant |
| R8329 |
T12664 |
T12657 |
prep |
at,Limbs |
| R8330 |
T12665 |
T12664 |
pobj |
E13.5,at |
| R8331 |
T12666 |
T12665 |
cc |
and,E13.5 |
| R8332 |
T12667 |
T12665 |
conj |
E14.5,E13.5 |
| R8333 |
T12668 |
T12658 |
auxpass |
were,dissected |
| R8334 |
T12669 |
T12658 |
cc |
and,dissected |
| R8335 |
T12670 |
T12658 |
conj |
frozen,dissected |
| R8336 |
T12671 |
T12670 |
prep |
in,frozen |
| R8337 |
T12672 |
T12671 |
pobj |
OCT,in |
| R8338 |
T12673 |
T12674 |
punct |
(,Finetek |
| R8339 |
T12674 |
T12670 |
parataxis |
Finetek,frozen |
| R8340 |
T12675 |
T12674 |
compound |
Sakura,Finetek |
| R8341 |
T12676 |
T12674 |
punct |
",",Finetek |
| R8342 |
T12677 |
T12674 |
npadvmod |
Torrence,Finetek |
| R8343 |
T12678 |
T12674 |
punct |
", ",Finetek |
| R8344 |
T12679 |
T12674 |
npadvmod |
CA,Finetek |
| R8345 |
T12680 |
T12674 |
punct |
", ",Finetek |
| R8346 |
T12681 |
T12682 |
compound |
United,States |
| R8347 |
T12682 |
T12674 |
npadvmod |
States,Finetek |
| R8348 |
T12683 |
T12674 |
punct |
),Finetek |
| R8349 |
T12684 |
T12658 |
punct |
.,dissected |
| R8350 |
T12686 |
T12687 |
nsubjpass |
Cryosections,assayed |
| R8351 |
T12688 |
T12686 |
prep |
of,Cryosections |
| R8352 |
T12689 |
T12688 |
pobj |
tissue,of |
| R8353 |
T12690 |
T12687 |
auxpass |
were,assayed |
| R8354 |
T12691 |
T12687 |
prep |
by,assayed |
| R8355 |
T12692 |
T12691 |
pobj |
TUNEL,by |
| R8356 |
T12693 |
T12687 |
advcl |
using,assayed |
| R8357 |
T12694 |
T12695 |
det |
the,Kit |
| R8358 |
T12695 |
T12693 |
dobj |
Kit,using |
| R8359 |
T12696 |
T12697 |
advmod |
In,Situ |
| R8360 |
T12697 |
T12698 |
amod |
Situ,Detection |
| R8361 |
T12698 |
T12695 |
compound |
Detection,Kit |
| R8362 |
T12699 |
T12700 |
compound |
Cell,Death |
| R8363 |
T12700 |
T12698 |
compound |
Death,Detection |
| R8364 |
T12701 |
T12695 |
punct |
", ",Kit |
| R8365 |
T12702 |
T12695 |
npadvmod |
Fluorescein,Kit |
| R8366 |
T12703 |
T12702 |
punct |
(,Fluorescein |
| R8367 |
T12704 |
T12702 |
npadvmod |
Roche,Fluorescein |
| R8368 |
T12705 |
T12702 |
punct |
", ",Fluorescein |
| R8369 |
T12706 |
T12702 |
npadvmod |
Basel,Fluorescein |
| R8370 |
T12707 |
T12702 |
punct |
", ",Fluorescein |
| R8371 |
T12708 |
T12702 |
npadvmod |
Switzerland,Fluorescein |
| R8372 |
T12709 |
T12702 |
punct |
),Fluorescein |
| R8373 |
T12710 |
T12687 |
punct |
.,assayed |
| R8374 |
T12712 |
T12713 |
prep |
Following,washed |
| R8375 |
T12714 |
T12712 |
pobj |
TUNEL,Following |
| R8376 |
T12715 |
T12713 |
punct |
", ",washed |
| R8377 |
T12716 |
T12713 |
nsubjpass |
slides,washed |
| R8378 |
T12717 |
T12713 |
auxpass |
were,washed |
| R8379 |
T12718 |
T12713 |
prep |
in,washed |
| R8380 |
T12719 |
T12718 |
pobj |
PBS,in |
| R8381 |
T12720 |
T12713 |
punct |
", ",washed |
| R8382 |
T12721 |
T12713 |
conj |
blocked,washed |
| R8383 |
T12722 |
T12721 |
prep |
with,blocked |
| R8384 |
T12723 |
T12722 |
pobj |
PBS,with |
| R8385 |
T12724 |
T12723 |
punct |
+,PBS |
| R8386 |
T12725 |
T12726 |
nummod |
0.05,% |
| R8387 |
T12726 |
T12727 |
compound |
%,Tween |
| R8388 |
T12727 |
T12723 |
appos |
Tween,PBS |
| R8389 |
T12728 |
T12727 |
punct |
-,Tween |
| R8390 |
T12729 |
T12727 |
nummod |
20,Tween |
| R8391 |
T12730 |
T12723 |
punct |
+,PBS |
| R8392 |
T12731 |
T12732 |
nummod |
5,% |
| R8393 |
T12732 |
T12733 |
compound |
%,serum |
| R8394 |
T12733 |
T12723 |
appos |
serum,PBS |
| R8395 |
T12734 |
T12733 |
compound |
goat,serum |
| R8396 |
T12735 |
T12721 |
punct |
", ",blocked |
| R8397 |
T12736 |
T12721 |
conj |
washed,blocked |
| R8398 |
T12737 |
T12736 |
advmod |
again,washed |
| R8399 |
T12738 |
T12736 |
punct |
", ",washed |
| R8400 |
T12739 |
T12736 |
cc |
and,washed |
| R8401 |
T12740 |
T12736 |
conj |
incubated,washed |
| R8402 |
T12741 |
T12740 |
prep |
with,incubated |
| R8403 |
T12742 |
T12743 |
det |
a,dilution |
| R8404 |
T12743 |
T12741 |
pobj |
dilution,with |
| R8405 |
T12744 |
T12743 |
nummod |
1,dilution |
| R8406 |
T12745 |
T12746 |
punct |
:,200 |
| R8407 |
T12746 |
T12744 |
prep |
200,1 |
| R8408 |
T12747 |
T12743 |
prep |
of,dilution |
| R8409 |
T12748 |
T12749 |
det |
a,antibody |
| R8410 |
T12749 |
T12747 |
pobj |
antibody,of |
| R8411 |
T12750 |
T12749 |
nmod |
rabbit,antibody |
| R8412 |
T12751 |
T12752 |
amod |
anti-phospho-histone,H3 |
| R8413 |
T12752 |
T12749 |
compound |
H3,antibody |
| R8414 |
T12753 |
T12752 |
punct |
-,H3 |
| R8415 |
T12754 |
T12749 |
acl |
called,antibody |
| R8416 |
T12755 |
T12756 |
compound |
Mitosis,Marker |
| R8417 |
T12756 |
T12754 |
oprd |
Marker,called |
| R8418 |
T12757 |
T12758 |
punct |
(,Biotechnology |
| R8419 |
T12758 |
T12756 |
parataxis |
Biotechnology,Marker |
| R8420 |
T12759 |
T12758 |
compound |
Upstate,Biotechnology |
| R8421 |
T12760 |
T12758 |
punct |
", ",Biotechnology |
| R8422 |
T12761 |
T12762 |
compound |
Lake,Placid |
| R8423 |
T12762 |
T12758 |
npadvmod |
Placid,Biotechnology |
| R8424 |
T12763 |
T12758 |
punct |
", ",Biotechnology |
| R8425 |
T12764 |
T12765 |
compound |
New,York |
| R8426 |
T12765 |
T12758 |
npadvmod |
York,Biotechnology |
| R8427 |
T12766 |
T12758 |
punct |
", ",Biotechnology |
| R8428 |
T12767 |
T12768 |
compound |
United,States |
| R8429 |
T12768 |
T12758 |
npadvmod |
States,Biotechnology |
| R8430 |
T12769 |
T12758 |
punct |
),Biotechnology |
| R8431 |
T12770 |
T12771 |
aux |
to,identify |
| R8432 |
T12771 |
T12740 |
advcl |
identify,incubated |
| R8433 |
T12772 |
T12771 |
dobj |
cells,identify |
| R8434 |
T12773 |
T12772 |
prep |
in,cells |
| R8435 |
T12774 |
T12773 |
pobj |
mitosis,in |
| R8436 |
T12775 |
T12713 |
punct |
.,washed |
| R8437 |
T12777 |
T12778 |
npadvmod |
Cy3,labeled |
| R8438 |
T12778 |
T12780 |
amod |
labeled,antibody |
| R8439 |
T12779 |
T12778 |
punct |
-,labeled |
| R8440 |
T12780 |
T12783 |
nsubjpass |
antibody,used |
| R8441 |
T12781 |
T12780 |
amod |
anti-rabbit,antibody |
| R8442 |
T12782 |
T12780 |
amod |
secondary,antibody |
| R8443 |
T12784 |
T12783 |
auxpass |
was,used |
| R8444 |
T12785 |
T12786 |
aux |
to,detect |
| R8445 |
T12786 |
T12783 |
advcl |
detect,used |
| R8446 |
T12787 |
T12788 |
det |
the,antibody |
| R8447 |
T12788 |
T12786 |
dobj |
antibody,detect |
| R8448 |
T12789 |
T12783 |
punct |
.,used |
| R8449 |
T12791 |
T12792 |
compound |
Cell,nuclei |
| R8450 |
T12792 |
T12793 |
nsubjpass |
nuclei,labeled |
| R8451 |
T12794 |
T12793 |
auxpass |
were,labeled |
| R8452 |
T12795 |
T12793 |
prep |
with,labeled |
| R8453 |
T12796 |
T12795 |
pobj |
DAPI,with |
| R8454 |
T12797 |
T12793 |
punct |
", ",labeled |
| R8455 |
T12798 |
T12793 |
cc |
and,labeled |
| R8456 |
T12799 |
T12800 |
nsubjpass |
slides,mounted |
| R8457 |
T12800 |
T12793 |
conj |
mounted,labeled |
| R8458 |
T12801 |
T12800 |
auxpass |
were,mounted |
| R8459 |
T12802 |
T12800 |
prep |
in,mounted |
| R8460 |
T12803 |
T12802 |
pobj |
Vectamount,in |
| R8461 |
T12804 |
T12805 |
punct |
(,Laboratories |
| R8462 |
T12805 |
T12803 |
parataxis |
Laboratories,Vectamount |
| R8463 |
T12806 |
T12805 |
compound |
Vector,Laboratories |
| R8464 |
T12807 |
T12805 |
punct |
", ",Laboratories |
| R8465 |
T12808 |
T12805 |
npadvmod |
Burlingame,Laboratories |
| R8466 |
T12809 |
T12805 |
punct |
", ",Laboratories |
| R8467 |
T12810 |
T12805 |
npadvmod |
California,Laboratories |
| R8468 |
T12811 |
T12805 |
punct |
", ",Laboratories |
| R8469 |
T12812 |
T12813 |
compound |
United,States |
| R8470 |
T12813 |
T12805 |
npadvmod |
States,Laboratories |
| R8471 |
T12814 |
T12805 |
punct |
),Laboratories |
| R8472 |
T12815 |
T12800 |
cc |
and,mounted |
| R8473 |
T12816 |
T12800 |
conj |
visualized,mounted |
| R8474 |
T12817 |
T12816 |
prep |
at,visualized |
| R8475 |
T12818 |
T12819 |
nummod |
100,magnification |
| R8476 |
T12819 |
T12817 |
pobj |
magnification,at |
| R8477 |
T12820 |
T12818 |
punct |
×,100 |
| R8478 |
T12821 |
T12800 |
punct |
.,mounted |
| R8479 |
T12823 |
T12824 |
det |
The,area |
| R8480 |
T12824 |
T12825 |
nsubjpass |
area,measured |
| R8481 |
T12826 |
T12824 |
prep |
of,area |
| R8482 |
T12827 |
T12828 |
amod |
selected,sites |
| R8483 |
T12828 |
T12826 |
pobj |
sites,of |
| R8484 |
T12829 |
T12828 |
amod |
anatomical,sites |
| R8485 |
T12830 |
T12825 |
auxpass |
were,measured |
| R8486 |
T12831 |
T12825 |
punct |
", ",measured |
| R8487 |
T12832 |
T12825 |
cc |
and,measured |
| R8488 |
T12833 |
T12834 |
det |
the,number |
| R8489 |
T12834 |
T12835 |
nsubjpass |
number,counted |
| R8490 |
T12835 |
T12825 |
conj |
counted,measured |
| R8491 |
T12836 |
T12834 |
prep |
of,number |
| R8492 |
T12837 |
T12838 |
npadvmod |
TUNEL,labeled |
| R8493 |
T12838 |
T12840 |
amod |
labeled,fragments |
| R8494 |
T12839 |
T12838 |
punct |
-,labeled |
| R8495 |
T12840 |
T12836 |
pobj |
fragments,of |
| R8496 |
T12841 |
T12840 |
amod |
nuclear,fragments |
| R8497 |
T12842 |
T12834 |
cc |
and,number |
| R8498 |
T12843 |
T12844 |
det |
the,number |
| R8499 |
T12844 |
T12834 |
conj |
number,number |
| R8500 |
T12845 |
T12844 |
prep |
of,number |
| R8501 |
T12846 |
T12847 |
npadvmod |
Cy3,labeled |
| R8502 |
T12847 |
T12849 |
amod |
labeled,nuclei |
| R8503 |
T12848 |
T12847 |
punct |
-,labeled |
| R8504 |
T12849 |
T12845 |
pobj |
nuclei,of |
| R8505 |
T12850 |
T12835 |
auxpass |
were,counted |
| R8506 |
T12851 |
T12835 |
prep |
from,counted |
| R8507 |
T12852 |
T12853 |
nummod |
three,sections |
| R8508 |
T12853 |
T12851 |
pobj |
sections,from |
| R8509 |
T12854 |
T12855 |
nummod |
10,μm |
| R8510 |
T12855 |
T12853 |
compound |
μm,sections |
| R8511 |
T12856 |
T12855 |
punct |
-,μm |
| R8512 |
T12857 |
T12853 |
acl |
spanning,sections |
| R8513 |
T12858 |
T12859 |
nummod |
50,μm |
| R8514 |
T12859 |
T12857 |
dobj |
μm,spanning |
| R8515 |
T12860 |
T12853 |
punct |
", ",sections |
| R8516 |
T12861 |
T12853 |
prep |
from,sections |
| R8517 |
T12862 |
T12863 |
nummod |
three,control |
| R8518 |
T12863 |
T12861 |
pobj |
control,from |
| R8519 |
T12864 |
T12863 |
cc |
and,control |
| R8520 |
T12865 |
T12866 |
nummod |
three,mutant |
| R8521 |
T12866 |
T12867 |
compound |
mutant,animals |
| R8522 |
T12867 |
T12863 |
conj |
animals,control |
| R8523 |
T12868 |
T12835 |
punct |
.,counted |
| R8524 |
T12870 |
T12871 |
det |
The,number |
| R8525 |
T12871 |
T12872 |
nsubjpass |
number,counted |
| R8526 |
T12873 |
T12871 |
prep |
of,number |
| R8527 |
T12874 |
T12875 |
amod |
labeled,cells |
| R8528 |
T12875 |
T12873 |
pobj |
cells,of |
| R8529 |
T12876 |
T12871 |
prep |
in,number |
| R8530 |
T12877 |
T12878 |
det |
the,joints |
| R8531 |
T12878 |
T12876 |
pobj |
joints,in |
| R8532 |
T12879 |
T12880 |
amod |
metacarpal,phalangeal |
| R8533 |
T12880 |
T12878 |
amod |
phalangeal,joints |
| R8534 |
T12881 |
T12880 |
punct |
-,phalangeal |
| R8535 |
T12882 |
T12880 |
cc |
and,phalangeal |
| R8536 |
T12883 |
T12884 |
amod |
metatarsal,phalangeal |
| R8537 |
T12884 |
T12880 |
conj |
phalangeal,phalangeal |
| R8538 |
T12885 |
T12884 |
punct |
-,phalangeal |
| R8539 |
T12886 |
T12872 |
auxpass |
was,counted |
| R8540 |
T12887 |
T12872 |
prep |
in,counted |
| R8541 |
T12888 |
T12889 |
det |
a,rectangle |
| R8542 |
T12889 |
T12887 |
pobj |
rectangle,in |
| R8543 |
T12890 |
T12891 |
nummod |
290,μm |
| R8544 |
T12891 |
T12889 |
nmod |
μm,rectangle |
| R8545 |
T12892 |
T12893 |
punct |
×,μm |
| R8546 |
T12893 |
T12891 |
prep |
μm,μm |
| R8547 |
T12894 |
T12893 |
nummod |
365,μm |
| R8548 |
T12895 |
T12889 |
acl |
placed,rectangle |
| R8549 |
T12896 |
T12895 |
prep |
around,placed |
| R8550 |
T12897 |
T12898 |
det |
the,center |
| R8551 |
T12898 |
T12896 |
pobj |
center,around |
| R8552 |
T12899 |
T12898 |
prep |
of,center |
| R8553 |
T12900 |
T12901 |
det |
the,joint |
| R8554 |
T12901 |
T12899 |
pobj |
joint,of |
| R8555 |
T12902 |
T12872 |
punct |
.,counted |
| R8556 |
T12904 |
T12905 |
det |
The,region |
| R8557 |
T12905 |
T12907 |
nsubjpass |
region,defined |
| R8558 |
T12906 |
T12905 |
amod |
posterior,region |
| R8559 |
T12908 |
T12905 |
prep |
of,region |
| R8560 |
T12909 |
T12910 |
det |
the,digit |
| R8561 |
T12910 |
T12908 |
pobj |
digit,of |
| R8562 |
T12911 |
T12910 |
amod |
fifth,digit |
| R8563 |
T12912 |
T12907 |
auxpass |
was,defined |
| R8564 |
T12913 |
T12907 |
prep |
by,defined |
| R8565 |
T12914 |
T12913 |
pcomp |
drawing,by |
| R8566 |
T12915 |
T12916 |
det |
a,line |
| R8567 |
T12916 |
T12914 |
dobj |
line,drawing |
| R8568 |
T12917 |
T12914 |
prep |
from,drawing |
| R8569 |
T12918 |
T12919 |
det |
the,tip |
| R8570 |
T12919 |
T12917 |
pobj |
tip,from |
| R8571 |
T12920 |
T12919 |
prep |
of,tip |
| R8572 |
T12921 |
T12922 |
det |
the,digit |
| R8573 |
T12922 |
T12920 |
pobj |
digit,of |
| R8574 |
T12923 |
T12914 |
prep |
down,drawing |
| R8575 |
T12924 |
T12925 |
nummod |
2.15,mm |
| R8576 |
T12925 |
T12923 |
pobj |
mm,down |
| R8577 |
T12926 |
T12923 |
cc |
and,down |
| R8578 |
T12927 |
T12923 |
conj |
across,down |
| R8579 |
T12928 |
T12927 |
prep |
to,across |
| R8580 |
T12929 |
T12930 |
det |
the,edge |
| R8581 |
T12930 |
T12928 |
pobj |
edge,to |
| R8582 |
T12931 |
T12930 |
amod |
lateral,edge |
| R8583 |
T12932 |
T12930 |
prep |
of,edge |
| R8584 |
T12933 |
T12934 |
det |
the,tissue |
| R8585 |
T12934 |
T12932 |
pobj |
tissue,of |
| R8586 |
T12935 |
T12907 |
punct |
.,defined |
| R8587 |
T12937 |
T12938 |
prep |
For,was |
| R8588 |
T12939 |
T12940 |
det |
this,analysis |
| R8589 |
T12940 |
T12937 |
pobj |
analysis,For |
| R8590 |
T12941 |
T12938 |
punct |
", ",was |
| R8591 |
T12942 |
T12943 |
det |
the,reporter |
| R8592 |
T12943 |
T12938 |
nsubj |
reporter,was |
| R8593 |
T12944 |
T12945 |
compound |
R26R,Cre |
| R8594 |
T12945 |
T12943 |
compound |
Cre,reporter |
| R8595 |
T12946 |
T12938 |
neg |
not,was |
| R8596 |
T12947 |
T12938 |
acomp |
present,was |
| R8597 |
T12948 |
T12938 |
punct |
.,was |