PMC:523229 / 53415-53764
Annnotations
craft-sa-dev
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Transgenic founder mice were identified by PCR using Cre-specific primers 5′-GCCTGCATTACCGGTCGATGCAACGA-3′ and 5′-GTGGCAGATGGCGCGGCAACACCATT-3′, which amplify a 725-bp product, and were assessed for absence of BAC vector using vector-specific primers 5′-CGGAGTCTGATGCGGTTGCGATG-3′ and 5′-AGTGCTGTTCCCTGGTGCTTCCTC-3′, which amplify a 465-bp product."}
craft-ca-core-ex-dev
{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T11295","span":{"begin":1,"end":11},"obj":"SO_EXT:transgenic_entity"},{"id":"T11296","span":{"begin":20,"end":24},"obj":"NCBITaxon:10088"},{"id":"T11297","span":{"begin":67,"end":74},"obj":"SO_EXT:0000112"},{"id":"T11298","span":{"begin":166,"end":168},"obj":"SO_EXT:0000028"},{"id":"T11299","span":{"begin":211,"end":221},"obj":"SO_EXT:0000153"},{"id":"T11300","span":{"begin":228,"end":234},"obj":"SO_EXT:0000440"},{"id":"T11301","span":{"begin":244,"end":251},"obj":"SO_EXT:0000112"},{"id":"T11302","span":{"begin":338,"end":340},"obj":"SO_EXT:0000028"}],"text":" Transgenic founder mice were identified by PCR using Cre-specific primers 5′-GCCTGCATTACCGGTCGATGCAACGA-3′ and 5′-GTGGCAGATGGCGCGGCAACACCATT-3′, which amplify a 725-bp product, and were assessed for absence of BAC vector using vector-specific primers 5′-CGGAGTCTGATGCGGTTGCGATG-3′ and 5′-AGTGCTGTTCCCTGGTGCTTCCTC-3′, which amplify a 465-bp product."}
craft-ca-core-dev
{"project":"craft-ca-core-dev","denotations":[{"id":"T11175","span":{"begin":20,"end":24},"obj":"NCBITaxon:10088"},{"id":"T11176","span":{"begin":67,"end":74},"obj":"SO:0000112"},{"id":"T11177","span":{"begin":166,"end":168},"obj":"SO:0000028"},{"id":"T11178","span":{"begin":211,"end":221},"obj":"SO:0000153"},{"id":"T11179","span":{"begin":228,"end":234},"obj":"SO:0000440"},{"id":"T11180","span":{"begin":244,"end":251},"obj":"SO:0000112"},{"id":"T11181","span":{"begin":338,"end":340},"obj":"SO:0000028"}],"text":" Transgenic founder mice were identified by PCR using Cre-specific primers 5′-GCCTGCATTACCGGTCGATGCAACGA-3′ and 5′-GTGGCAGATGGCGCGGCAACACCATT-3′, which amplify a 725-bp product, and were assessed for absence of BAC vector using vector-specific primers 5′-CGGAGTCTGATGCGGTTGCGATG-3′ and 5′-AGTGCTGTTCCCTGGTGCTTCCTC-3′, which amplify a 465-bp product."}