PMC:523229 / 13981-15380 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/523229","sourcedb":"PMC","sourceid":"523229","source_url":"http://www.ncbi.nlm.nih.gov/pmc/523229","text":"Figure 2 Bmpr1a Is Required for Webbing Regression and Apoptosis in Specific Regions of the Limb\n(A and B) Control E14.5 forelimb (A) compared to a, E14.5 mutant forelimb (B) showing webbing between digits 1 and 2 (arrowheads) and extra tissue at the posterior of digit 5 (arrows).\n(C) Gdf5-Cre induced lacZ expression from R26R in an E13.5 forelimb showing LACZ staining (blue) in metacarpal-phalangeal joints, between digits 1 and 2 (arrowhead), and in a region posterior to digit 5 (arrow).\n(D and E) Sections of E14.5 hindlimbs showing apoptosis visualized by TUNEL staining (green) and proliferation visualized by staining for histone H3 phosphorylation (red). Controls show strong, uniform TUNEL staining between digits 1 and 2 (D, arrowhead) while mutants show patchy TUNEL staining interspersed with mitotic cells in similar regions (E). Scale bar = 200 μm.\n(F) Quantitation of TUNEL staining and mitotic cells in the posterior region of the fifth digit shows apoptosis is reduced 30% while proliferation is increased 20% (asterisks indicate statistically significant difference).\n(G and H) By E15.5, interdigital tissue has regressed in controls (G, arrowhead). In contrast, tissue remains in mutants at this location, primarily derived from cells that have undergone Gdf5-Cre-mediated recombination that inactivates Bmpr1a function and activates expression of LACZ (H). Scale bar = 75 μm.","divisions":[{"label":"label","span":{"begin":0,"end":8}},{"label":"title","span":{"begin":10,"end":98}},{"label":"p","span":{"begin":98,"end":282}},{"label":"p","span":{"begin":283,"end":494}},{"label":"p","span":{"begin":495,"end":866}},{"label":"p","span":{"begin":867,"end":1089}}],"tracks":[]}