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Id Subject Object Predicate Lexical cue
T2809 3328-3329 . denotes .
T2808 3321-3323 NN denotes RT
T2807 3324-3328 NN denotes step
T2806 3317-3320 DT denotes the
T2805 3314-3316 IN denotes of
T2804 3305-3313 NN denotes omission
T2803 3302-3304 IN denotes by
T2802 3296-3300 CD denotes 0.02
T2801 3300-3301 NN denotes %
T2800 3295-3296 SYM denotes <
T2799 3292-3294 VB denotes be
T2798 3289-3291 TO denotes to
T2797 3276-3279 VBD denotes was
T2796 3268-3275 NNS denotes samples
T2795 3264-3267 NN denotes ear
T2794 3258-3263 JJ denotes inner
T2793 3255-3257 IN denotes of
T2792 3280-3288 VBN denotes assessed
T2791 3241-3254 NN denotes contamination
T2790 3233-3240 JJ denotes Genomic
T2789 3232-3329 sentence denotes Genomic contamination of inner ear samples was assessed to be <0.02% by omission of the RT step.
T2788 3231-3232 . denotes .
T2787 3224-3231 NNS denotes amounts
T2786 3217-3223 NN denotes tissue
T2785 3209-3216 VB denotes compare
T2784 3206-3208 TO denotes to
T2783 3196-3200 VBD denotes were
T2782 3191-3195 NN denotes rRNA
T2781 3187-3190 NN denotes 18S
T2780 3184-3186 IN denotes of
T2740 3013-3014 SYM denotes ×
T2739 3018-3019 CD denotes 4
T2738 3008-3012 CD denotes 3.39
T2737 3006-3007 SYM denotes +
T2736 3001-3002 SYM denotes *
T2735 3003-3005 NN denotes S4
T2734 2998-2999 HYPH denotes -
T2733 2996-2998 CD denotes 10
T2732 2994-2995 SYM denotes ×
T2731 2999-3000 CD denotes 3
T2730 2988-2993 CD denotes 11.38
T2729 2986-2987 SYM denotes +
T2728 2981-2982 SYM denotes ×
T2727 2983-2985 NN denotes S3
T2726 2976-2980 CD denotes 0.15
T2725 2974-2975 SYM denotes +
T2724 2969-2970 SYM denotes ×
T2723 2971-2973 NN denotes S2
T2722 2964-2968 CD denotes 1.05
T2721 2962-2963 SYM denotes +
T2720 2958-2959 SYM denotes ×
T2719 2960-2961 NN denotes S
T2718 2953-2957 CD denotes 3.80
T2717 2951-2952 SYM denotes +
T2716 2944-2945 SYM denotes =
T2715 2946-2950 CD denotes 7.39
T2714 2942-2943 NN denotes F
T2713 2940-2942 : denotes :
T2712 2923-2924 HYPH denotes -
T2711 2924-2929 NN denotes order
T2710 2918-2923 JJ denotes fifth
T2709 2913-2914 HYPH denotes -
T2708 2914-2917 NN denotes fit
T2707 2909-2913 JJ denotes best
T2706 2930-2940 NN denotes polynomial
T2705 2907-2908 DT denotes a
T2704 2903-2906 IN denotes via
T2703 2890-2896 NN denotes growth
T2702 2897-2902 NN denotes curve
T2701 2886-2889 DT denotes the
T2700 2883-2885 IN denotes of
T2699 2869-2870 SYM denotes -
T2698 2870-2876 JJ denotes linear
T2697 2866-2869 NN denotes log
T2696 2877-2882 NN denotes phase
T2695 2862-2865 DT denotes the
T2694 2859-2861 IN denotes of
T2693 2857-2858 -RRB- denotes )
T2692 2856-2857 NN denotes S
T2691 2855-2856 -LRB- denotes (
T2690 2849-2854 NN denotes slope
T2689 2845-2848 DT denotes the
T2688 2840-2844 IN denotes from
T2687 2827-2830 VBD denotes was
T2686 2825-2826 -RRB- denotes )
T2685 2824-2825 NN denotes F
T2684 2823-2824 -LRB- denotes (
T2683 2831-2839 VBN denotes obtained
T2682 2814-2822 NN denotes Fidelity
T2681 2813-3025 sentence denotes Fidelity (F) was obtained from the slope (S) of the log-linear phase of the growth curve via a best-fit fifth-order polynomial: F = 7.39 + 3.80 × S + 1.05 × S2 + 0.15 × S3 + 11.38 × 10-3 * S4 + 3.39 × 10-4 × S5.
T2680 2812-2813 . denotes .
T2679 2811-2812 -RRB- denotes ]
T2678 2809-2811 CD denotes 15
T2677 2808-2809 -LRB- denotes [
T2676 2791-2797 VBN denotes chosen
T2675 2798-2807 NN denotes threshold
T2674 2789-2790 DT denotes a
T2673 2771-2780 NNS denotes molecules
T2672 2763-2770 NN denotes product
T2671 2760-2762 IN denotes of
T2670 2753-2759 NN denotes number
T2669 2749-2752 DT denotes the
T2668 2743-2748 WDT denotes which
T2667 2781-2788 VBZ denotes reaches
T2666 2740-2742 IN denotes at
T2665 2734-2739 NN denotes cycle
T2664 2730-2733 DT denotes the
T2663 2727-2729 VBZ denotes is
T2662 2724-2726 NN denotes Ct
T2661 2720-2723 CC denotes and
T2660 2711-2719 NN denotes reaction
T2659 2707-2710 DT denotes the
T2658 2704-2706 IN denotes of
T2657 2695-2703 NN denotes fidelity
T2656 2691-2694 DT denotes the
T2655 2688-2690 VBZ denotes is
T2654 2686-2687 NN denotes F
T2653 2684-2686 , denotes ,
T2652 2675-2684 NNS denotes molecules
T2651 2667-2674 NN denotes product
T2650 2664-2666 IN denotes of
T2649 2657-2663 NN denotes number
T2648 2653-2656 DT denotes the
T2647 2648-2649 NN denotes P
T2646 2650-2652 VBZ denotes is
T2645 2642-2647 WRB denotes where
T2644 2640-2641 -RRB- denotes )
T2643 2637-2638 SYM denotes ^
T2642 2635-2636 NN denotes F
T2641 2634-2635 -LRB- denotes (
T2640 2638-2640 NN denotes Ct
T2639 2632-2633 SYM denotes /
T2638 2628-2629 SYM denotes =
T2637 2630-2631 NN denotes P
T2636 2626-2627 NN denotes T
T2635 2623-2625 IN denotes to
T2634 2613-2622 VBG denotes according
T2633 2597-2601 VBD denotes were
T2632 2595-2596 -RRB- denotes )
T2631 2594-2595 NN denotes T
T2630 2593-2594 -LRB- denotes (
T2629 2602-2612 VBN denotes quantified
T2628 2583-2592 NNS denotes molecules
T2626 1975-2813 sentence denotes Table 1 Primers Template Primers Product Length Melting Temperature Hot Measurement Fidelity n 18S gag gtt cga aga cga tca ga (sense) 316 bp 83.2 ± 02°C 78°C 1.89 ± 0.02 17 tcg ctc cac caa cta aga ac (antisense) KCNJ10 tgg tgt ggt gtg gta tct gg (sense) 411 bp 83.2 ± 02°C 78°C 1.86 ± 0.02 15 tga agc agt ttg cct gtc ac (antisense) KCNQ1 ttt gtt cat ccc cat ctc ag (sense) 239 bp 82.5 ± 02°C 79°C 1.85 ± 0.02 17 gtt gct ggg tag gaa gag (antisense) KCNQ4 ccc gga aac cct tct gtg tc (sense) 245 bp 83.2 ± 02°C 80°C 1.87 ± 0.01 17 aaa gat gag cac cag gaa cc (antisense) Template molecules (T) were quantified according to T = P / (F ^Ct) where P is the number of product molecules, F is the fidelity of the reaction and Ct is the cycle at which the number of product molecules reaches a chosen threshold [15].
T2779 3201-3205 VBN denotes used
T2778 3168-3183 NNS denotes Quantifications
T2777 3167-3232 sentence denotes Quantifications of 18S rRNA were used to compare tissue amounts.
T2776 3166-3167 . denotes .
T2775 3163-3164 SYM denotes ^
T2774 3164-3166 NN denotes Ct
T2773 3161-3162 NN denotes F
T2772 3159-3160 SYM denotes ×
T2771 3155-3156 SYM denotes =
T2770 3157-3158 NN denotes T
T2769 3151-3154 NN denotes PCt
T2768 3148-3150 IN denotes to
T2767 3138-3147 VBG denotes according
T2766 3133-3137 NN denotes rRNA
T2765 3129-3132 NN denotes 18S
T2764 3126-3128 IN denotes of
T2763 3118-3125 NNS denotes amounts
T2762 3112-3117 VBN denotes known
T2761 3109-3111 IN denotes of
T2760 3095-3108 NN denotes amplification
T2759 3092-3094 IN denotes by
T2758 3077-3080 VBD denotes was
T2757 3075-3076 -RRB- denotes )
T2756 3072-3075 NN denotes PCt
T2755 3071-3072 -LRB- denotes (
T2754 3061-3070 NN denotes threshold
T2753 3058-3060 IN denotes at
T2752 3048-3057 NNS denotes molecules
T2751 3040-3047 NN denotes product
T2750 3037-3039 IN denotes of
T2749 3081-3091 VBN denotes determined
T2748 3030-3036 NN denotes number
T2747 3026-3029 DT denotes The
T2746 3025-3167 sentence denotes The number of product molecules at threshold (PCt) was determined by amplification of known amounts of 18S rRNA according to PCt = T × F ^Ct.
T2745 3024-3025 . denotes .
T2744 3020-3021 SYM denotes ×
T2743 3022-3024 NN denotes S5
T2742 3017-3018 HYPH denotes -
T2741 3015-3017 CD denotes 10
T2210 0-12 JJ denotes Quantitative
T2211 16-19 NN denotes PCR
T2212 13-15 NN denotes RT
T2213 15-16 HYPH denotes -
T2214 19-96 sentence denotes Animals were deeply anesthetized with sodium pentobarbital (100 mg/kg i.p.).
T2215 20-27 NNS denotes Animals
T2216 40-52 VBN denotes anesthetized
T2217 28-32 VBD denotes were
T2218 33-39 RB denotes deeply
T2219 53-57 IN denotes with
T2220 58-64 NN denotes sodium
T2221 65-78 NN denotes pentobarbital
T2222 79-80 -LRB- denotes (
T2223 84-86 NN denotes mg
T2224 80-83 CD denotes 100
T2225 86-87 SYM denotes /
T2226 87-89 NN denotes kg
T2227 90-94 RB denotes i.p.
T2228 94-95 -RRB- denotes )
T2229 95-96 . denotes .
T2230 96-156 sentence denotes Kidneys and brain were removed and pulverized in liquid N2.
T2231 97-104 NNS denotes Kidneys
T2232 120-127 VBN denotes removed
T2233 105-108 CC denotes and
T2234 109-114 NN denotes brain
T2235 115-119 VBD denotes were
T2236 128-131 CC denotes and
T2237 132-142 VBN denotes pulverized
T2238 143-145 IN denotes in
T2239 146-152 NN denotes liquid
T2240 153-155 NN denotes N2
T2241 155-156 . denotes .
T2242 156-384 sentence denotes Temporal bones were removed and two preparations of inner ear tissues were obtained by microdissection: (1) stria vascularis without spiral ligament, and (2) spiral ganglia including the surrounding bone and the organ of Corti.
T2243 157-165 JJ denotes Temporal
T2244 166-171 NNS denotes bones
T2245 177-184 VBN denotes removed
T2246 172-176 VBD denotes were
T2247 185-188 CC denotes and
T2248 189-192 CD denotes two
T2249 193-205 NNS denotes preparations
T2250 232-240 VBN denotes obtained
T2251 206-208 IN denotes of
T2252 209-214 JJ denotes inner
T2253 219-226 NNS denotes tissues
T2254 215-218 NN denotes ear
T2255 227-231 VBD denotes were
T2256 241-243 IN denotes by
T2257 244-259 NN denotes microdissection
T2258 259-261 : denotes :
T2259 261-262 -LRB- denotes (
T2260 262-263 LS denotes 1
T2261 271-281 NN denotes vascularis
T2262 263-264 -RRB- denotes )
T2263 265-270 NN denotes stria
T2264 282-289 IN denotes without
T2265 290-296 JJ denotes spiral
T2266 297-305 NN denotes ligament
T2267 305-307 , denotes ,
T2268 307-310 CC denotes and
T2269 311-312 -LRB- denotes (
T2270 312-313 LS denotes 2
T2271 322-329 NNS denotes ganglia
T2272 313-314 -RRB- denotes )
T2273 315-321 JJ denotes spiral
T2274 330-339 VBG denotes including
T2275 340-343 DT denotes the
T2276 356-360 NN denotes bone
T2277 344-355 VBG denotes surrounding
T2278 361-364 CC denotes and
T2279 365-368 DT denotes the
T2280 369-374 NN denotes organ
T2281 375-377 IN denotes of
T2282 378-383 NNP denotes Corti
T2283 383-384 . denotes .
T2284 384-529 sentence denotes The dissection medium was changed twice during the microdissection and isolated tissues were washed three times to minimize cross-contamination.
T2285 385-388 DT denotes The
T2286 400-406 NN denotes medium
T2287 389-399 NN denotes dissection
T2288 411-418 VBN denotes changed
T2289 407-410 VBD denotes was
T2290 419-424 RB denotes twice
T2291 425-431 IN denotes during
T2292 432-435 DT denotes the
T2293 436-451 NN denotes microdissection
T2294 452-455 CC denotes and
T2295 456-464 VBN denotes isolated
T2296 465-472 NNS denotes tissues
T2297 478-484 VBN denotes washed
T2298 473-477 VBD denotes were
T2299 485-490 CD denotes three
T2300 491-496 NNS denotes times
T2301 497-499 TO denotes to
T2302 500-508 VB denotes minimize
T2303 509-528 NN denotes cross-contamination
T2304 528-529 . denotes .
T2305 529-632 sentence denotes The Slc26a4-/-genotype was verified by the observation of large otoconia in the utricular macula [14].
T2306 530-533 DT denotes The
T2307 544-552 NN denotes genotype
T2308 534-541 NN denotes Slc26a4
T2309 541-542 SYM denotes -
T2310 542-543 HYPH denotes /
T2311 543-544 SYM denotes -
T2312 557-565 VBN denotes verified
T2313 553-556 VBD denotes was
T2314 566-568 IN denotes by
T2315 569-572 DT denotes the
T2316 573-584 NN denotes observation
T2317 585-587 IN denotes of
T2318 588-593 JJ denotes large
T2319 594-602 NN denotes otoconia
T2320 603-605 IN denotes in
T2321 606-609 DT denotes the
T2322 620-626 NN denotes macula
T2323 610-619 JJ denotes utricular
T2324 627-628 -LRB- denotes [
T2325 628-630 CD denotes 14
T2326 630-631 -RRB- denotes ]
T2327 631-632 . denotes .
T2328 632-759 sentence denotes Total RNA was isolated and residual DNA contamination was removed by enzymatic digestion (RNeasy micro, Qiagen, Valencia, CA).
T2329 633-638 JJ denotes Total
T2330 639-642 NN denotes RNA
T2331 647-655 VBN denotes isolated
T2332 643-646 VBD denotes was
T2333 656-659 CC denotes and
T2334 660-668 JJ denotes residual
T2335 673-686 NN denotes contamination
T2336 669-672 NN denotes DNA
T2337 691-698 VBN denotes removed
T2338 687-690 VBD denotes was
T2339 699-701 IN denotes by
T2340 702-711 JJ denotes enzymatic
T2341 712-721 NN denotes digestion
T2342 722-723 -LRB- denotes (
T2343 730-735 NN denotes micro
T2344 723-729 NN denotes RNeasy
T2345 735-737 , denotes ,
T2346 737-743 NNP denotes Qiagen
T2347 743-745 , denotes ,
T2348 745-753 NNP denotes Valencia
T2349 753-755 , denotes ,
T2350 755-757 NNP denotes CA
T2351 757-758 -RRB- denotes )
T2352 758-759 . denotes .
T2353 759-896 sentence denotes Quality and quantity of 18S rRNA obtained from kidneys and brain were determined (Nano Assay, 2100 Bioanalyzer, Agilent, Palo Alto, CA).
T2354 760-767 NN denotes Quality
T2355 830-840 VBN denotes determined
T2356 768-771 CC denotes and
T2357 772-780 NN denotes quantity
T2358 781-783 IN denotes of
T2359 784-787 NN denotes 18S
T2360 788-792 NN denotes rRNA
T2361 793-801 VBN denotes obtained
T2362 802-806 IN denotes from
T2363 807-814 NNS denotes kidneys
T2364 815-818 CC denotes and
T2365 819-824 NN denotes brain
T2366 825-829 VBD denotes were
T2367 841-842 -LRB- denotes (
T2368 847-852 NNP denotes Assay
T2369 842-846 NNP denotes Nano
T2370 852-854 , denotes ,
T2371 854-858 CD denotes 2100
T2372 859-870 NNP denotes Bioanalyzer
T2373 870-872 , denotes ,
T2374 872-879 NNP denotes Agilent
T2375 879-881 , denotes ,
T2376 881-885 NNP denotes Palo
T2377 886-890 NNP denotes Alto
T2378 890-892 , denotes ,
T2379 892-894 NNP denotes CA
T2380 894-895 -RRB- denotes )
T2381 895-896 . denotes .
T2382 896-1032 sentence denotes Further, the quality of RNA preparations obtained from stria vascularis and spiral ganglia was assessed (Pico Assay, 2100 Bioanalyzer).
T2383 897-904 RB denotes Further
T2384 992-1000 VBN denotes assessed
T2385 904-906 , denotes ,
T2386 906-909 DT denotes the
T2387 910-917 NN denotes quality
T2388 918-920 IN denotes of
T2389 921-924 NN denotes RNA
T2390 925-937 NNS denotes preparations
T2391 938-946 VBN denotes obtained
T2392 947-951 IN denotes from
T2393 952-957 NN denotes stria
T2394 958-968 NN denotes vascularis
T2395 969-972 CC denotes and
T2396 973-979 JJ denotes spiral
T2397 980-987 NNS denotes ganglia
T2398 988-991 VBD denotes was
T2399 1001-1002 -LRB- denotes (
T2400 1007-1012 NNP denotes Assay
T2401 1002-1006 NNP denotes Pico
T2402 1012-1014 , denotes ,
T2403 1014-1018 CD denotes 2100
T2404 1019-1030 NNP denotes Bioanalyzer
T2405 1030-1031 -RRB- denotes )
T2406 1031-1032 . denotes .
T2407 1032-1223 sentence denotes Real time RT-PCR was performed on RNA obtained from individual animals (OneStep RT-PCR, Qiagen; Smart Cycler, Cepheid, Sunnyvale, CA) in the presence of 0.2× SYBR green I (Molecular Probes).
T2408 1033-1037 JJ denotes Real
T2409 1038-1042 NN denotes time
T2410 1046-1049 NN denotes PCR
T2411 1043-1045 NN denotes RT
T2412 1045-1046 HYPH denotes -
T2413 1054-1063 VBN denotes performed
T2414 1050-1053 VBD denotes was
T2415 1064-1066 IN denotes on
T2416 1067-1070 NN denotes RNA
T2417 1071-1079 VBN denotes obtained
T2418 1080-1084 IN denotes from
T2419 1085-1095 JJ denotes individual
T2420 1096-1103 NNS denotes animals
T2421 1104-1105 -LRB- denotes (
T2422 1135-1141 NNP denotes Cycler
T2423 1105-1112 NNP denotes OneStep
T2424 1116-1119 NN denotes PCR
T2425 1113-1115 NN denotes RT
T2426 1115-1116 HYPH denotes -
T2427 1119-1121 , denotes ,
T2428 1121-1127 NNP denotes Qiagen
T2429 1127-1128 : denotes ;
T2430 1129-1134 NNP denotes Smart
T2431 1141-1143 , denotes ,
T2432 1143-1150 NNP denotes Cepheid
T2433 1150-1152 , denotes ,
T2434 1152-1161 NNP denotes Sunnyvale
T2435 1161-1163 , denotes ,
T2436 1163-1165 NNP denotes CA
T2437 1165-1166 -RRB- denotes )
T2438 1167-1169 IN denotes in
T2439 1170-1173 DT denotes the
T2440 1174-1182 NN denotes presence
T2441 1183-1185 IN denotes of
T2442 1186-1189 CD denotes 0.2
T2443 1191-1195 NN denotes SYBR
T2444 1189-1190 SYM denotes ×
T2445 1202-1203 NN denotes I
T2446 1196-1201 JJ denotes green
T2447 1204-1205 -LRB- denotes (
T2448 1215-1221 NNP denotes Probes
T2449 1205-1214 NNP denotes Molecular
T2450 1221-1222 -RRB- denotes )
T2451 1222-1223 . denotes .
T2452 1223-1354 sentence denotes Transcripts of 18S rRNA and mRNA for the K+ channels KCNJ10, KCNQ1 and KCNQ4 were amplified using gene-specific primers (Table 1).
T2453 1224-1235 NNS denotes Transcripts
T2454 1306-1315 VBN denotes amplified
T2455 1236-1238 IN denotes of
T2456 1239-1242 NN denotes 18S
T2457 1243-1247 NN denotes rRNA
T2458 1248-1251 CC denotes and
T2459 1252-1256 NN denotes mRNA
T2460 1257-1260 IN denotes for
T2461 1261-1264 DT denotes the
T2462 1268-1276 NNS denotes channels
T2463 1265-1266 NN denotes K
T2464 1266-1267 SYM denotes +
T2465 1277-1283 NN denotes KCNJ10
T2466 1283-1285 , denotes ,
T2467 1285-1290 NN denotes KCNQ1
T2468 1291-1294 CC denotes and
T2469 1295-1300 NN denotes KCNQ4
T2470 1301-1305 VBD denotes were
T2471 1316-1321 VBG denotes using
T2472 1322-1326 NN denotes gene
T2473 1327-1335 JJ denotes specific
T2474 1326-1327 HYPH denotes -
T2475 1336-1343 NNS denotes primers
T2476 1344-1345 -LRB- denotes (
T2477 1345-1350 NN denotes Table
T2478 1351-1352 CD denotes 1
T2479 1352-1353 -RRB- denotes )
T2480 1353-1354 . denotes .
T2481 1354-1410 sentence denotes RT was performed for 30 min at 50°C and 15 min at 95°C.
T2482 1355-1357 NN denotes RT
T2483 1362-1371 VBN denotes performed
T2484 1358-1361 VBD denotes was
T2485 1372-1375 IN denotes for
T2486 1376-1378 CD denotes 30
T2487 1379-1382 NN denotes min
T2488 1383-1385 IN denotes at
T2489 1386-1388 CD denotes 50
T2490 1388-1390 NN denotes °C
T2491 1391-1394 CC denotes and
T2492 1395-1397 CD denotes 15
T2493 1398-1401 NN denotes min
T2494 1402-1404 IN denotes at
T2495 1405-1407 CD denotes 95
T2496 1407-1409 NN denotes °C
T2497 1409-1410 . denotes .
T2498 1410-1605 sentence denotes PCR consisted of 50 cycles of 1 min at 60°C, 1 min at 72°C, 7s heating to hot-measurement temperature, 13s hot-measurement at 3–5°C below product melting temperature (Table 1) and 1 min at 94°C.
T2499 1411-1414 NN denotes PCR
T2500 1415-1424 VBD denotes consisted
T2501 1425-1427 IN denotes of
T2502 1428-1430 CD denotes 50
T2503 1431-1437 NNS denotes cycles
T2504 1438-1440 IN denotes of
T2505 1441-1442 CD denotes 1
T2506 1443-1446 NN denotes min
T2507 1447-1449 IN denotes at
T2508 1450-1452 CD denotes 60
T2509 1452-1454 NN denotes °C
T2510 1454-1456 , denotes ,
T2511 1456-1457 CD denotes 1
T2512 1458-1461 NN denotes min
T2513 1462-1464 IN denotes at
T2514 1465-1467 CD denotes 72
T2515 1467-1469 NN denotes °C
T2516 1469-1471 , denotes ,
T2517 1471-1473 NN denotes 7s
T2518 1474-1481 VBG denotes heating
T2519 1482-1484 IN denotes to
T2520 1485-1488 JJ denotes hot
T2521 1489-1500 NN denotes measurement
T2522 1488-1489 HYPH denotes -
T2523 1501-1512 NN denotes temperature
T2524 1512-1514 , denotes ,
T2525 1514-1517 NNS denotes 13s
T2526 1522-1533 NN denotes measurement
T2527 1518-1521 NN denotes hot
T2528 1521-1522 HYPH denotes -
T2529 1534-1536 IN denotes at
T2530 1537-1538 CD denotes 3
T2531 1539-1540 CD denotes 5
T2532 1538-1539 SYM denotes –
T2533 1540-1542 NN denotes °C
T2534 1543-1548 IN denotes below
T2535 1549-1556 NN denotes product
T2536 1565-1576 NN denotes temperature
T2537 1557-1564 NN denotes melting
T2538 1577-1578 -LRB- denotes (
T2539 1578-1583 NN denotes Table
T2540 1584-1585 CD denotes 1
T2541 1585-1586 -RRB- denotes )
T2542 1587-1590 CC denotes and
T2543 1591-1592 CD denotes 1
T2544 1593-1596 NN denotes min
T2545 1597-1599 IN denotes at
T2546 1600-1602 CD denotes 94
T2547 1602-1604 NN denotes °C
T2548 1604-1605 . denotes .
T2549 1605-1737 sentence denotes Hot-measurements were performed to eliminate the detection of primer-dimers that had melting temperatures between 72 and 75°C [15].
T2550 1606-1609 JJ denotes Hot
T2551 1610-1622 NNS denotes measurements
T2552 1609-1610 HYPH denotes -
T2553 1628-1637 VBN denotes performed
T2554 1623-1627 VBD denotes were
T2555 1638-1640 TO denotes to
T2556 1641-1650 VB denotes eliminate
T2557 1651-1654 DT denotes the
T2558 1655-1664 NN denotes detection
T2559 1665-1667 IN denotes of
T2560 1668-1674 NN denotes primer
T2561 1675-1681 NNS denotes dimers
T2562 1674-1675 HYPH denotes -
T2563 1682-1686 WDT denotes that
T2564 1687-1690 VBD denotes had
T2565 1691-1698 NN denotes melting
T2566 1699-1711 NNS denotes temperatures
T2567 1712-1719 IN denotes between
T2568 1720-1722 CD denotes 72
T2569 1729-1731 NN denotes °C
T2570 1723-1726 CC denotes and
T2571 1727-1729 CD denotes 75
T2572 1732-1733 -LRB- denotes [
T2573 1733-1735 CD denotes 15
T2574 1735-1736 -RRB- denotes ]
T2575 1736-1737 . denotes .
T2576 1737-1778 sentence denotes PCR was followed by a melt (60 to 95°C).
T2577 1738-1741 NN denotes PCR
T2578 1746-1754 VBN denotes followed
T2579 1742-1745 VBD denotes was
T2580 1755-1757 IN denotes by
T2581 1758-1759 DT denotes a
T2582 1760-1764 NN denotes melt
T2583 1765-1766 -LRB- denotes (
T2584 1774-1776 NN denotes °C
T2585 1766-1768 CD denotes 60
T2586 1772-1774 CD denotes 95
T2592 1783-1793 NN denotes generation
T2593 1848-1855 VBN denotes checked
T2594 1794-1796 IN denotes of
T2595 1797-1798 DT denotes a
T2596 1806-1813 NN denotes product
T2597 1799-1805 JJ denotes single
T2598 1814-1816 IN denotes of
T2599 1817-1828 JJ denotes appropriate
T2600 1829-1833 NN denotes size
T2601 1834-1837 VBD denotes was
T2602 1838-1847 RB denotes routinely
T2603 1856-1858 IN denotes by
T2604 1859-1862 DT denotes the
T2605 1863-1871 NN denotes presence
T2606 1872-1874 IN denotes of
T2607 1875-1876 DT denotes a
T2608 1889-1893 NN denotes peak
T2609 1877-1883 JJ denotes single
T2610 1884-1888 NN denotes melt
T2611 1894-1897 CC denotes and
T2612 1898-1900 IN denotes by
T2613 1901-1908 NN denotes agarose
T2614 1909-1912 NN denotes gel
T2615 1913-1928 NN denotes electrophoresis
T2616 1912-1913 HYPH denotes -
T2617 1928-1929 . denotes .
T2618 1929-1975 sentence denotes Product identity was confirmed by sequencing.
T2619 1930-1937 NN denotes Product
T2620 1938-1946 NN denotes identity
T2621 1951-1960 VBN denotes confirmed
T2622 1947-1950 VBD denotes was
T2623 1961-1963 IN denotes by
T2624 1964-1974 NN denotes sequencing
T2625 1974-1975 . denotes .
T2627 1976-2001 NN denotes Table 1 Primers Template
T7759 1985-1992 NNS denotes Primers
T2587 1769-1771 IN denotes to
T2588 1776-1777 -RRB- denotes )
T2589 1777-1778 . denotes .
T2590 1778-1929 sentence denotes The generation of a single product of appropriate size was routinely checked by the presence of a single melt peak and by agarose gel-electrophoresis.
T2591 1779-1782 DT denotes The
R1298 T2210 T2211 amod Quantitative,PCR
R1299 T2212 T2211 compound RT,PCR
R1300 T2213 T2211 punct -,PCR
R1301 T2215 T2216 nsubjpass Animals,anesthetized
R1302 T2217 T2216 auxpass were,anesthetized
R1303 T2218 T2216 advmod deeply,anesthetized
R1304 T2219 T2216 prep with,anesthetized
R1305 T2220 T2221 compound sodium,pentobarbital
R1306 T2221 T2219 pobj pentobarbital,with
R1307 T2222 T2223 punct (,mg
R1308 T2223 T2221 parataxis mg,pentobarbital
R1309 T2224 T2223 nummod 100,mg
R1310 T2225 T2226 punct /,kg
R1311 T2226 T2223 prep kg,mg
R1312 T2227 T2223 advmod i.p.,mg
R1313 T2228 T2223 punct ),mg
R1314 T2229 T2216 punct .,anesthetized
R1315 T2231 T2232 nsubjpass Kidneys,removed
R1316 T2233 T2231 cc and,Kidneys
R1317 T2234 T2231 conj brain,Kidneys
R1318 T2235 T2232 auxpass were,removed
R1319 T2236 T2232 cc and,removed
R1320 T2237 T2232 conj pulverized,removed
R1321 T2238 T2237 prep in,pulverized
R1322 T2239 T2240 compound liquid,N2
R1323 T2240 T2238 pobj N2,in
R1324 T2241 T2232 punct .,removed
R1325 T2243 T2244 amod Temporal,bones
R1326 T2244 T2245 nsubjpass bones,removed
R1327 T2246 T2245 auxpass were,removed
R1328 T2247 T2245 cc and,removed
R1329 T2248 T2249 nummod two,preparations
R1330 T2249 T2250 nsubjpass preparations,obtained
R1331 T2250 T2245 conj obtained,removed
R1332 T2251 T2249 prep of,preparations
R1333 T2252 T2253 amod inner,tissues
R1334 T2253 T2251 pobj tissues,of
R1335 T2254 T2253 compound ear,tissues
R1336 T2255 T2250 auxpass were,obtained
R1337 T2256 T2250 prep by,obtained
R1338 T2257 T2256 pobj microdissection,by
R1339 T2258 T2250 punct : ,obtained
R1340 T2259 T2260 punct (,1
R1341 T2260 T2261 meta 1,vascularis
R1342 T2261 T2250 dobj vascularis,obtained
R1343 T2262 T2260 punct ),1
R1344 T2263 T2261 compound stria,vascularis
R1345 T2264 T2261 prep without,vascularis
R1346 T2265 T2266 amod spiral,ligament
R1347 T2266 T2264 pobj ligament,without
R1348 T2267 T2261 punct ", ",vascularis
R1349 T2268 T2261 cc and,vascularis
R1350 T2269 T2270 punct (,2
R1351 T2270 T2271 meta 2,ganglia
R1352 T2271 T2261 conj ganglia,vascularis
R1353 T2272 T2270 punct ),2
R1354 T2273 T2271 amod spiral,ganglia
R1355 T2274 T2271 prep including,ganglia
R1356 T2275 T2276 det the,bone
R1357 T2276 T2274 pobj bone,including
R1358 T2277 T2276 amod surrounding,bone
R1359 T2278 T2276 cc and,bone
R1360 T2279 T2280 det the,organ
R1361 T2280 T2276 conj organ,bone
R1362 T2281 T2280 prep of,organ
R1363 T2282 T2281 pobj Corti,of
R1364 T2283 T2250 punct .,obtained
R1365 T2285 T2286 det The,medium
R1366 T2286 T2288 nsubjpass medium,changed
R1367 T2287 T2286 compound dissection,medium
R1368 T2289 T2288 auxpass was,changed
R1369 T2290 T2288 advmod twice,changed
R1370 T2291 T2288 prep during,changed
R1371 T2292 T2293 det the,microdissection
R1372 T2293 T2291 pobj microdissection,during
R1373 T2294 T2288 cc and,changed
R1374 T2295 T2296 amod isolated,tissues
R1375 T2296 T2297 nsubjpass tissues,washed
R1376 T2297 T2288 conj washed,changed
R1377 T2298 T2297 auxpass were,washed
R1378 T2299 T2300 nummod three,times
R1379 T2300 T2297 npadvmod times,washed
R1380 T2301 T2302 aux to,minimize
R1381 T2302 T2297 advcl minimize,washed
R1382 T2303 T2302 dobj cross-contamination,minimize
R1383 T2304 T2297 punct .,washed
R1384 T2306 T2307 det The,genotype
R1385 T2307 T2312 nsubjpass genotype,verified
R1386 T2308 T2307 nmod Slc26a4,genotype
R1387 T2309 T2308 punct -,Slc26a4
R1388 T2310 T2308 punct /,Slc26a4
R1389 T2311 T2308 punct -,Slc26a4
R1390 T2313 T2312 auxpass was,verified
R1391 T2314 T2312 prep by,verified
R1392 T2315 T2316 det the,observation
R1393 T2316 T2314 pobj observation,by
R1394 T2317 T2316 prep of,observation
R1395 T2318 T2319 amod large,otoconia
R1396 T2319 T2317 pobj otoconia,of
R1397 T2320 T2316 prep in,observation
R1398 T2321 T2322 det the,macula
R1399 T2322 T2320 pobj macula,in
R1400 T2323 T2322 amod utricular,macula
R1401 T2324 T2325 punct [,14
R1402 T2325 T2312 parataxis 14,verified
R1403 T2326 T2325 punct ],14
R1404 T2327 T2312 punct .,verified
R1405 T2329 T2330 amod Total,RNA
R1406 T2330 T2331 nsubjpass RNA,isolated
R1407 T2332 T2331 auxpass was,isolated
R1408 T2333 T2331 cc and,isolated
R1409 T2334 T2335 amod residual,contamination
R1410 T2335 T2337 nsubjpass contamination,removed
R1411 T2336 T2335 compound DNA,contamination
R1412 T2337 T2331 conj removed,isolated
R1413 T2338 T2337 auxpass was,removed
R1414 T2339 T2337 prep by,removed
R1415 T2340 T2341 amod enzymatic,digestion
R1416 T2341 T2339 pobj digestion,by
R1417 T2342 T2343 punct (,micro
R1418 T2343 T2337 parataxis micro,removed
R1419 T2344 T2343 compound RNeasy,micro
R1420 T2345 T2343 punct ", ",micro
R1421 T2346 T2343 npadvmod Qiagen,micro
R1422 T2347 T2343 punct ", ",micro
R1423 T2348 T2343 npadvmod Valencia,micro
R1424 T2349 T2343 punct ", ",micro
R1425 T2350 T2343 npadvmod CA,micro
R1426 T2351 T2343 punct ),micro
R1427 T2352 T2337 punct .,removed
R1428 T2354 T2355 nsubjpass Quality,determined
R1429 T2356 T2354 cc and,Quality
R1430 T2357 T2354 conj quantity,Quality
R1431 T2358 T2354 prep of,Quality
R1432 T2359 T2360 compound 18S,rRNA
R1433 T2360 T2358 pobj rRNA,of
R1434 T2361 T2360 acl obtained,rRNA
R1435 T2362 T2361 prep from,obtained
R1436 T2363 T2362 pobj kidneys,from
R1437 T2364 T2363 cc and,kidneys
R1438 T2365 T2363 conj brain,kidneys
R1439 T2366 T2355 auxpass were,determined
R1440 T2367 T2368 punct (,Assay
R1441 T2368 T2355 parataxis Assay,determined
R1442 T2369 T2368 compound Nano,Assay
R1443 T2370 T2368 punct ", ",Assay
R1444 T2371 T2372 nummod 2100,Bioanalyzer
R1445 T2372 T2368 appos Bioanalyzer,Assay
R1446 T2373 T2368 punct ", ",Assay
R1447 T2374 T2368 npadvmod Agilent,Assay
R1448 T2375 T2368 punct ", ",Assay
R1449 T2376 T2377 compound Palo,Alto
R1450 T2377 T2368 npadvmod Alto,Assay
R1451 T2378 T2368 punct ", ",Assay
R1452 T2379 T2368 npadvmod CA,Assay
R1453 T2380 T2368 punct ),Assay
R1454 T2381 T2355 punct .,determined
R1455 T2383 T2384 advmod Further,assessed
R1456 T2385 T2384 punct ", ",assessed
R1457 T2386 T2387 det the,quality
R1458 T2387 T2384 nsubjpass quality,assessed
R1459 T2388 T2387 prep of,quality
R1460 T2389 T2390 compound RNA,preparations
R1461 T2390 T2388 pobj preparations,of
R1462 T2391 T2390 acl obtained,preparations
R1463 T2392 T2391 prep from,obtained
R1464 T2393 T2394 compound stria,vascularis
R1465 T2394 T2392 pobj vascularis,from
R1466 T2395 T2394 cc and,vascularis
R1467 T2396 T2397 amod spiral,ganglia
R1468 T2397 T2394 conj ganglia,vascularis
R1469 T2398 T2384 auxpass was,assessed
R1470 T2399 T2400 punct (,Assay
R1471 T2400 T2384 parataxis Assay,assessed
R1472 T2401 T2400 compound Pico,Assay
R1473 T2402 T2400 punct ", ",Assay
R1474 T2403 T2404 nummod 2100,Bioanalyzer
R1475 T2404 T2400 appos Bioanalyzer,Assay
R1476 T2405 T2400 punct ),Assay
R1477 T2406 T2384 punct .,assessed
R1478 T2408 T2409 amod Real,time
R1479 T2409 T2410 compound time,PCR
R1480 T2410 T2413 nsubjpass PCR,performed
R1481 T2411 T2410 compound RT,PCR
R1482 T2412 T2410 punct -,PCR
R1483 T2414 T2413 auxpass was,performed
R1484 T2415 T2413 prep on,performed
R1485 T2416 T2415 pobj RNA,on
R1486 T2417 T2416 acl obtained,RNA
R1487 T2418 T2417 prep from,obtained
R1488 T2419 T2420 amod individual,animals
R1489 T2420 T2418 pobj animals,from
R1490 T2421 T2422 punct (,Cycler
R1491 T2422 T2417 parataxis Cycler,obtained
R1492 T2423 T2424 compound OneStep,PCR
R1493 T2424 T2422 dep PCR,Cycler
R1494 T2425 T2424 compound RT,PCR
R1495 T2426 T2424 punct -,PCR
R1496 T2427 T2424 punct ", ",PCR
R1497 T2428 T2424 npadvmod Qiagen,PCR
R1498 T2429 T2422 punct ;,Cycler
R1499 T2430 T2422 compound Smart,Cycler
R1500 T2431 T2422 punct ", ",Cycler
R1501 T2432 T2422 npadvmod Cepheid,Cycler
R1502 T2433 T2422 punct ", ",Cycler
R1503 T2434 T2422 npadvmod Sunnyvale,Cycler
R1504 T2435 T2422 punct ", ",Cycler
R1505 T2436 T2422 npadvmod CA,Cycler
R1506 T2437 T2422 punct ),Cycler
R1507 T2438 T2413 prep in,performed
R1508 T2439 T2440 det the,presence
R1509 T2440 T2438 pobj presence,in
R1510 T2441 T2440 prep of,presence
R1511 T2442 T2443 nummod 0.2,SYBR
R1512 T2443 T2445 nmod SYBR,I
R1513 T2444 T2443 punct ×,SYBR
R1514 T2445 T2441 pobj I,of
R1515 T2446 T2445 amod green,I
R1516 T2447 T2448 punct (,Probes
R1517 T2448 T2445 parataxis Probes,I
R1518 T2449 T2448 compound Molecular,Probes
R1519 T2450 T2448 punct ),Probes
R1520 T2451 T2413 punct .,performed
R1521 T2453 T2454 nsubjpass Transcripts,amplified
R1522 T2455 T2453 prep of,Transcripts
R1523 T2456 T2457 compound 18S,rRNA
R1524 T2457 T2455 pobj rRNA,of
R1525 T2458 T2457 cc and,rRNA
R1526 T2459 T2457 conj mRNA,rRNA
R1527 T2460 T2453 prep for,Transcripts
R1528 T2461 T2462 det the,channels
R1529 T2462 T2460 pobj channels,for
R1530 T2463 T2462 nmod K,channels
R1531 T2464 T2463 punct +,K
R1532 T2465 T2462 appos KCNJ10,channels
R1533 T2466 T2465 punct ", ",KCNJ10
R1534 T2467 T2465 conj KCNQ1,KCNJ10
R1535 T2468 T2467 cc and,KCNQ1
R1536 T2469 T2467 conj KCNQ4,KCNQ1
R1537 T2470 T2454 auxpass were,amplified
R1538 T2471 T2454 advcl using,amplified
R1539 T2472 T2473 npadvmod gene,specific
R1540 T2473 T2475 amod specific,primers
R1541 T2474 T2473 punct -,specific
R1542 T2475 T2471 dobj primers,using
R1543 T2476 T2477 punct (,Table
R1544 T2477 T2454 parataxis Table,amplified
R1545 T2478 T2477 nummod 1,Table
R1546 T2479 T2477 punct ),Table
R1547 T2480 T2454 punct .,amplified
R1548 T2482 T2483 nsubjpass RT,performed
R1549 T2484 T2483 auxpass was,performed
R1550 T2485 T2483 prep for,performed
R1551 T2486 T2487 nummod 30,min
R1552 T2487 T2485 pobj min,for
R1553 T2488 T2483 prep at,performed
R1554 T2489 T2490 nummod 50,°C
R1555 T2490 T2488 pobj °C,at
R1556 T2491 T2483 cc and,performed
R1557 T2492 T2493 nummod 15,min
R1558 T2493 T2483 conj min,performed
R1559 T2494 T2493 prep at,min
R1560 T2495 T2496 nummod 95,°C
R1561 T2496 T2494 pobj °C,at
R1562 T2497 T2483 punct .,performed
R1563 T2499 T2500 nsubj PCR,consisted
R1564 T2501 T2500 prep of,consisted
R1565 T2502 T2503 nummod 50,cycles
R1566 T2503 T2501 pobj cycles,of
R1567 T2504 T2503 prep of,cycles
R1568 T2505 T2506 nummod 1,min
R1569 T2506 T2504 pobj min,of
R1570 T2507 T2506 prep at,min
R1571 T2508 T2509 nummod 60,°C
R1572 T2509 T2507 pobj °C,at
R1573 T2510 T2506 punct ", ",min
R1574 T2511 T2512 nummod 1,min
R1575 T2512 T2506 conj min,min
R1576 T2513 T2512 prep at,min
R1577 T2514 T2515 nummod 72,°C
R1578 T2515 T2513 pobj °C,at
R1579 T2516 T2512 punct ", ",min
R1580 T2517 T2512 conj 7s,min
R1581 T2518 T2517 amod heating,7s
R1582 T2519 T2517 prep to,7s
R1583 T2520 T2521 amod hot,measurement
R1584 T2521 T2523 compound measurement,temperature
R1585 T2522 T2521 punct -,measurement
R1586 T2523 T2519 pobj temperature,to
R1587 T2524 T2517 punct ", ",7s
R1588 T2525 T2526 compound 13s,measurement
R1589 T2526 T2517 conj measurement,7s
R1590 T2527 T2526 compound hot,measurement
R1591 T2528 T2526 punct -,measurement
R1592 T2529 T2526 prep at,measurement
R1593 T2530 T2531 quantmod 3,5
R1594 T2531 T2533 nummod 5,°C
R1595 T2532 T2531 punct –,5
R1596 T2533 T2534 npadvmod °C,below
R1597 T2534 T2529 prep below,at
R1598 T2535 T2536 compound product,temperature
R1599 T2536 T2534 pobj temperature,below
R1600 T2537 T2536 compound melting,temperature
R1601 T2538 T2539 punct (,Table
R1602 T2539 T2536 parataxis Table,temperature
R1603 T2540 T2539 nummod 1,Table
R1604 T2541 T2539 punct ),Table
R1605 T2542 T2526 cc and,measurement
R1606 T2543 T2544 nummod 1,min
R1607 T2544 T2526 conj min,measurement
R1608 T2545 T2544 prep at,min
R1609 T2546 T2547 nummod 94,°C
R1610 T2547 T2545 pobj °C,at
R1611 T2548 T2500 punct .,consisted
R1612 T2550 T2551 amod Hot,measurements
R1613 T2551 T2553 nsubjpass measurements,performed
R1614 T2552 T2551 punct -,measurements
R1615 T2554 T2553 auxpass were,performed
R1616 T2555 T2556 aux to,eliminate
R1617 T2556 T2553 advcl eliminate,performed
R1618 T2557 T2558 det the,detection
R1619 T2558 T2556 dobj detection,eliminate
R1620 T2559 T2558 prep of,detection
R1621 T2560 T2561 compound primer,dimers
R1622 T2561 T2559 pobj dimers,of
R1623 T2562 T2561 punct -,dimers
R1624 T2563 T2564 dep that,had
R1625 T2564 T2561 relcl had,dimers
R1626 T2565 T2566 compound melting,temperatures
R1627 T2566 T2564 dobj temperatures,had
R1628 T2567 T2564 prep between,had
R1629 T2568 T2569 nummod 72,°C
R1630 T2569 T2567 pobj °C,between
R1631 T2570 T2568 cc and,72
R1632 T2571 T2568 conj 75,72
R1633 T2572 T2573 punct [,15
R1634 T2573 T2564 parataxis 15,had
R1635 T2574 T2573 punct ],15
R1636 T2575 T2553 punct .,performed
R1637 T2577 T2578 nsubjpass PCR,followed
R1638 T2579 T2578 auxpass was,followed
R1639 T2580 T2578 agent by,followed
R1640 T2581 T2582 det a,melt
R1641 T2582 T2580 pobj melt,by
R1642 T2583 T2584 punct (,°C
R1643 T2584 T2582 parataxis °C,melt
R1644 T2585 T2586 quantmod 60,95
R1645 T2586 T2584 nummod 95,°C
R1646 T2587 T2586 quantmod to,95
R1647 T2588 T2584 punct ),°C
R1648 T2589 T2578 punct .,followed
R1649 T2591 T2592 det The,generation
R1650 T2592 T2593 nsubjpass generation,checked
R1651 T2594 T2592 prep of,generation
R1652 T2595 T2596 det a,product
R1653 T2596 T2594 pobj product,of
R1654 T2597 T2596 amod single,product
R1655 T2598 T2596 prep of,product
R1656 T2599 T2600 amod appropriate,size
R1657 T2600 T2598 pobj size,of
R1658 T2601 T2593 auxpass was,checked
R1659 T2602 T2593 advmod routinely,checked
R1660 T2603 T2593 prep by,checked
R1661 T2604 T2605 det the,presence
R1662 T2605 T2603 pobj presence,by
R1663 T2606 T2605 prep of,presence
R1664 T2607 T2608 det a,peak
R1665 T2608 T2606 pobj peak,of
R1666 T2609 T2608 amod single,peak
R1667 T2610 T2608 compound melt,peak
R1668 T2611 T2603 cc and,by
R1669 T2612 T2603 conj by,by
R1670 T2613 T2614 compound agarose,gel
R1671 T2614 T2615 compound gel,electrophoresis
R1673 T2616 T2615 punct -,electrophoresis
R1674 T2617 T2593 punct .,checked
R1675 T2619 T2620 compound Product,identity
R1676 T2620 T2621 nsubjpass identity,confirmed
R1677 T2622 T2621 auxpass was,confirmed
R1678 T2623 T2621 prep by,confirmed
R1679 T2624 T2623 pobj sequencing,by
R1680 T2625 T2621 punct .,confirmed
R1681 T2627 T2628 compound "Table 1 Primers Template",molecules
R1682 T2628 T2629 nsubjpass molecules,quantified
R1683 T2630 T2628 punct (,molecules
R1684 T2631 T2628 appos T,molecules
R1685 T2632 T2628 punct ),molecules
R1686 T2633 T2629 auxpass were,quantified
R1687 T2634 T2629 prep according,quantified
R1688 T2635 T2634 prep to,according
R1689 T2636 T2637 nsubj T,P
R1690 T2637 T2635 pcomp P,to
R1691 T2638 T2637 punct =,P
R1692 T2639 T2640 punct /,Ct
R1693 T2640 T2637 prep Ct,P
R1694 T2641 T2640 punct (,Ct
R1695 T2642 T2640 nmod F,Ct
R1696 T2643 T2640 punct ^,Ct
R1697 T2644 T2640 punct ),Ct
R1698 T2645 T2646 advmod where,is
R1699 T2646 T2637 advcl is,P
R1700 T2647 T2646 nsubj P,is
R1701 T2648 T2649 det the,number
R1702 T2649 T2646 attr number,is
R1703 T2650 T2649 prep of,number
R1704 T2651 T2652 compound product,molecules
R1705 T2652 T2650 pobj molecules,of
R1706 T2653 T2646 punct ", ",is
R1707 T2654 T2655 nsubj F,is
R1708 T2655 T2646 conj is,is
R1709 T2656 T2657 det the,fidelity
R1710 T2657 T2655 attr fidelity,is
R1711 T2658 T2657 prep of,fidelity
R1712 T2659 T2660 det the,reaction
R1713 T2660 T2658 pobj reaction,of
R1714 T2661 T2655 cc and,is
R1715 T2662 T2663 nsubj Ct,is
R1716 T2663 T2655 conj is,is
R1717 T2664 T2665 det the,cycle
R1718 T2665 T2663 attr cycle,is
R1719 T2666 T2667 prep at,reaches
R1720 T2667 T2665 relcl reaches,cycle
R1721 T2668 T2666 pobj which,at
R1722 T2669 T2670 det the,number
R1723 T2670 T2667 nsubj number,reaches
R1724 T2671 T2670 prep of,number
R1725 T2672 T2673 compound product,molecules
R1726 T2673 T2671 pobj molecules,of
R1727 T2674 T2675 det a,threshold
R1728 T2675 T2667 dobj threshold,reaches
R1729 T2676 T2675 amod chosen,threshold
R1730 T2677 T2678 punct [,15
R1731 T2678 T2646 parataxis 15,is
R1732 T2679 T2678 punct ],15
R1733 T2680 T2629 punct .,quantified
R1734 T2682 T2683 nsubjpass Fidelity,obtained
R1735 T2684 T2682 punct (,Fidelity
R1736 T2685 T2682 appos F,Fidelity
R1737 T2686 T2682 punct ),Fidelity
R1738 T2687 T2683 auxpass was,obtained
R1739 T2688 T2683 prep from,obtained
R1740 T2689 T2690 det the,slope
R1741 T2690 T2688 pobj slope,from
R1742 T2691 T2690 punct (,slope
R1743 T2692 T2690 appos S,slope
R1744 T2693 T2690 punct ),slope
R1745 T2694 T2690 prep of,slope
R1746 T2695 T2696 det the,phase
R1747 T2696 T2694 pobj phase,of
R1748 T2697 T2698 npadvmod log,linear
R1749 T2698 T2696 amod linear,phase
R1750 T2699 T2698 punct -,linear
R1751 T2700 T2696 prep of,phase
R1752 T2701 T2702 det the,curve
R1753 T2702 T2700 pobj curve,of
R1754 T2703 T2702 compound growth,curve
R1755 T2704 T2683 prep via,obtained
R1756 T2705 T2706 det a,polynomial
R1757 T2706 T2704 pobj polynomial,via
R1758 T2707 T2708 amod best,fit
R1759 T2708 T2706 nmod fit,polynomial
R1760 T2709 T2708 punct -,fit
R1761 T2710 T2711 amod fifth,order
R1762 T2711 T2706 compound order,polynomial
R1763 T2712 T2711 punct -,order
R1764 T2713 T2706 punct : ,polynomial
R1765 T2714 T2715 nsubj F,7.39
R1766 T2715 T2706 appos 7.39,polynomial
R1767 T2716 T2715 punct =,7.39
R1768 T2717 T2715 punct +,7.39
R1769 T2718 T2719 nummod 3.80,S
R1770 T2719 T2715 appos S,7.39
R1771 T2720 T2719 punct ×,S
R1772 T2721 T2715 punct +,7.39
R1773 T2722 T2723 nummod 1.05,S2
R1774 T2723 T2715 appos S2,7.39
R1775 T2724 T2723 punct ×,S2
R1776 T2725 T2715 punct +,7.39
R1777 T2726 T2727 nummod 0.15,S3
R1778 T2727 T2715 appos S3,7.39
R1779 T2728 T2727 punct ×,S3
R1780 T2729 T2715 punct +,7.39
R1781 T2730 T2731 quantmod 11.38,3
R1782 T2731 T2735 nummod 3,S4
R1783 T2732 T2731 punct ×,3
R1784 T2733 T2731 compound 10,3
R1785 T2734 T2731 punct -,3
R1786 T2735 T2715 appos S4,7.39
R1787 T2736 T2735 punct *,S4
R1788 T2737 T2715 punct +,7.39
R1789 T2738 T2739 quantmod 3.39,4
R1790 T2739 T2743 nummod 4,S5
R1791 T2740 T2739 punct ×,4
R1792 T2741 T2739 compound 10,4
R1793 T2742 T2739 punct -,4
R1794 T2743 T2715 appos S5,7.39
R1795 T2744 T2743 punct ×,S5
R1796 T2745 T2683 punct .,obtained
R1797 T2747 T2748 det The,number
R1798 T2748 T2749 nsubjpass number,determined
R1799 T2750 T2748 prep of,number
R1800 T2751 T2752 compound product,molecules
R1801 T2752 T2750 pobj molecules,of
R1802 T2753 T2752 prep at,molecules
R1803 T2754 T2753 pobj threshold,at
R1804 T2755 T2752 punct (,molecules
R1805 T2756 T2752 appos PCt,molecules
R1806 T2757 T2752 punct ),molecules
R1807 T2758 T2749 auxpass was,determined
R1808 T2759 T2749 prep by,determined
R1809 T2760 T2759 pobj amplification,by
R1810 T2761 T2760 prep of,amplification
R1811 T2762 T2763 amod known,amounts
R1812 T2763 T2761 pobj amounts,of
R1813 T2764 T2763 prep of,amounts
R1814 T2765 T2766 compound 18S,rRNA
R1815 T2766 T2764 pobj rRNA,of
R1816 T2767 T2760 prep according,amplification
R1817 T2768 T2767 prep to,according
R1818 T2769 T2770 nsubj PCt,T
R1819 T2770 T2768 pcomp T,to
R1820 T2771 T2770 punct =,T
R1821 T2772 T2770 punct ×,T
R1822 T2773 T2774 nmod F,Ct
R1823 T2774 T2770 appos Ct,T
R1824 T2775 T2774 punct ^,Ct
R1825 T2776 T2749 punct .,determined
R1826 T2778 T2779 nsubjpass Quantifications,used
R1827 T2780 T2778 prep of,Quantifications
R1828 T2781 T2782 compound 18S,rRNA
R1829 T2782 T2780 pobj rRNA,of
R1830 T2783 T2779 auxpass were,used
R1831 T2784 T2785 aux to,compare
R1832 T2785 T2779 advcl compare,used
R1833 T2786 T2787 compound tissue,amounts
R1834 T2787 T2785 dobj amounts,compare
R1835 T2788 T2779 punct .,used
R1836 T2790 T2791 amod Genomic,contamination
R1837 T2791 T2792 nsubjpass contamination,assessed
R1838 T2793 T2791 prep of,contamination
R1839 T2794 T2795 amod inner,ear
R1840 T2795 T2796 compound ear,samples
R1841 T2796 T2793 pobj samples,of
R1842 T2797 T2792 auxpass was,assessed
R1843 T2798 T2799 aux to,be
R1844 T2799 T2792 xcomp be,assessed
R1845 T2800 T2801 punct <,%
R1846 T2801 T2799 acomp %,be
R1847 T2802 T2801 nummod 0.02,%
R1848 T2803 T2792 prep by,assessed
R1849 T2804 T2803 pobj omission,by
R1850 T2805 T2804 prep of,omission
R1851 T2806 T2807 det the,step
R1852 T2807 T2805 pobj step,of
R1853 T2808 T2807 compound RT,step
R1854 T2809 T2792 punct .,assessed
R1672 T2615 T2612 pobj electrophoresis,by

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2209 3321-3323 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T2208 3258-3267 UBERON:0001846 denotes inner ear
T2207 3233-3240 SO_EXT:0001026 denotes Genomic
T2206 3217-3223 UBERON:0000479 denotes tissue
T2205 3191-3195 CHEBI_SO_EXT:rRNA denotes rRNA
T2204 3187-3195 SO_EXT:0000407 denotes 18S rRNA
T2203 3133-3137 CHEBI_SO_EXT:rRNA denotes rRNA
T2202 3129-3137 SO_EXT:0000407 denotes 18S rRNA
T2201 3048-3057 CHEBI:36357 denotes molecules
T2200 2771-2780 CHEBI:36357 denotes molecules
T2199 2675-2684 CHEBI:36357 denotes molecules
T2198 2583-2592 CHEBI:36357 denotes molecules
T2140 13-15 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T2141 20-27 NCBITaxon:33208 denotes Animals
T2142 40-52 CHEBI_EXT:anaesthetic_process denotes anesthetized
T2143 58-78 CHEBI:7984 denotes sodium pentobarbital
T2144 97-104 UBERON:0002113 denotes Kidneys
T2145 109-114 UBERON:0000955 denotes brain
T2146 153-155 CHEBI:17997 denotes N2
T2147 157-171 UBERON:0001678 denotes Temporal bones
T2148 209-218 UBERON:0001846 denotes inner ear
T2149 219-226 UBERON:0000479 denotes tissues
T2150 265-281 UBERON:0002282 denotes stria vascularis
T2151 290-305 UBERON:0006725 denotes spiral ligament
T2152 315-329 UBERON:0000395 denotes spiral ganglia
T2153 356-360 UBERON_EXT:bone_element_or_tissue denotes bone
T2154 369-383 UBERON:0002227 denotes organ of Corti
T2155 465-472 UBERON:0000479 denotes tissues
T2156 534-541 PR_EXT:000015039 denotes Slc26a4
T2157 541-542 SO_EXT:sequence_nullness_or_absence denotes -
T2158 543-544 SO_EXT:sequence_nullness_or_absence denotes -
T2159 544-552 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T2160 594-602 UBERON:0002280 denotes otoconia
T2161 610-626 UBERON:0002214 denotes utricular macula
T2162 639-642 CHEBI_SO_EXT:RNA denotes RNA
T2163 669-672 CHEBI_SO_EXT:DNA denotes DNA
T2164 702-711 CHEBI_GO_SO_EXT:enzyme denotes enzymatic
T2165 784-792 SO_EXT:0000407 denotes 18S rRNA
T2167 807-814 UBERON:0002113 denotes kidneys
T2168 819-824 UBERON:0000955 denotes brain
T2169 921-924 CHEBI_SO_EXT:RNA denotes RNA
T2170 952-968 UBERON:0002282 denotes stria vascularis
T2171 973-987 UBERON:0000395 denotes spiral ganglia
T2172 1043-1045 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T2173 1067-1070 CHEBI_SO_EXT:RNA denotes RNA
T2174 1096-1103 NCBITaxon:33208 denotes animals
T2175 1113-1115 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T2176 1191-1203 CHEBI:51461 denotes SYBR green I
T2177 1205-1214 CHEBI_EXT:polyatomic_entity_or_group denotes Molecular
T2178 1215-1221 CHEBI_SO_EXT:molecular_probe denotes Probes
T2179 1224-1235 SO_EXT:0000673 denotes Transcripts
T2180 1239-1247 SO_EXT:0000407 denotes 18S rRNA
T2181 1243-1247 CHEBI_SO_EXT:rRNA denotes rRNA
T2182 1252-1256 CHEBI_SO_EXT:mRNA denotes mRNA
T2183 1265-1267 CHEBI:29103 denotes K+
T2184 1265-1276 GO_EXT:0005267 denotes K+ channels
T2185 1277-1283 PR_EXT:000001979 denotes KCNJ10
T2186 1285-1290 PR_EXT:000000728 denotes KCNQ1
T2187 1295-1300 PR_EXT:000000731 denotes KCNQ4
T2188 1322-1326 SO_EXT:0000704 denotes gene
T2189 1336-1343 SO_EXT:0000112 denotes primers
T2190 1355-1357 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T2191 1557-1564 GO:0032508 denotes melting
T2192 1668-1674 SO_EXT:0000112 denotes primer
T2193 1675-1681 GO_EXT:dimer_macromolecular_complex denotes dimers
T2194 1691-1698 GO:0032508 denotes melting
T2195 1760-1764 GO:0032508 denotes melt
T2196 1884-1888 GO:0032508 denotes melt
T2197 1901-1908 CHEBI:2511 denotes agarose
T7758 1985-1992 SO_EXT:0000112 denotes Primers
T2166 788-792 CHEBI_SO_EXT:rRNA denotes rRNA

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2139 3258-3267 UBERON:0001846 denotes inner ear
T2138 3233-3240 SO:0001026 denotes Genomic
T2137 3217-3223 UBERON:0000479 denotes tissue
T2136 3187-3195 SO:0000407 denotes 18S rRNA
T2135 3129-3137 SO:0000407 denotes 18S rRNA
T2134 3048-3057 CHEBI:36357 denotes molecules
T2133 2771-2780 CHEBI:36357 denotes molecules
T2132 2675-2684 CHEBI:36357 denotes molecules
T2131 2583-2592 CHEBI:36357 denotes molecules
T2094 20-27 NCBITaxon:33208 denotes Animals
T2095 58-78 CHEBI:7984 denotes sodium pentobarbital
T2096 97-104 UBERON:0002113 denotes Kidneys
T2097 109-114 UBERON:0000955 denotes brain
T2098 153-155 CHEBI:17997 denotes N2
T2099 157-171 UBERON:0001678 denotes Temporal bones
T2100 209-218 UBERON:0001846 denotes inner ear
T2101 219-226 UBERON:0000479 denotes tissues
T2102 265-281 UBERON:0002282 denotes stria vascularis
T2103 290-305 UBERON:0006725 denotes spiral ligament
T2104 315-329 UBERON:0000395 denotes spiral ganglia
T2105 369-383 UBERON:0002227 denotes organ of Corti
T2106 465-472 UBERON:0000479 denotes tissues
T2107 534-541 PR:000015039 denotes Slc26a4
T2108 594-602 UBERON:0002280 denotes otoconia
T2109 610-626 UBERON:0002214 denotes utricular macula
T2110 784-792 SO:0000407 denotes 18S rRNA
T2111 807-814 UBERON:0002113 denotes kidneys
T2112 819-824 UBERON:0000955 denotes brain
T2113 952-968 UBERON:0002282 denotes stria vascularis
T2114 973-987 UBERON:0000395 denotes spiral ganglia
T2115 1096-1103 NCBITaxon:33208 denotes animals
T2116 1191-1203 CHEBI:51461 denotes SYBR green I
T2117 1224-1235 SO:0000673 denotes Transcripts
T2118 1239-1247 SO:0000407 denotes 18S rRNA
T2119 1265-1267 CHEBI:29103 denotes K+
T2120 1277-1283 PR:000001979 denotes KCNJ10
T2121 1285-1290 PR:000000728 denotes KCNQ1
T2122 1295-1300 PR:000000731 denotes KCNQ4
T2123 1322-1326 SO:0000704 denotes gene
T2124 1336-1343 SO:0000112 denotes primers
T2125 1557-1564 GO:0032508 denotes melting
T2126 1668-1674 SO:0000112 denotes primer
T2127 1691-1698 GO:0032508 denotes melting
T2128 1760-1764 GO:0032508 denotes melt
T2129 1884-1888 GO:0032508 denotes melt
T2130 1901-1908 CHEBI:2511 denotes agarose
T7757 1985-1992 SO:0000112 denotes Primers

2_test

Id Subject Object Predicate Lexical cue
15320950-11152663-12070954 628-630 11152663 denotes 14
15320950-10079033-12070955 1733-1735 10079033 denotes 15
15320950-10079033-12070956 2809-2811 10079033 denotes 15