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Id Subject Object Predicate Lexical cue
T3300 7982-7993 NNS denotes experiments
T3299 7979-7981 IN denotes of
T3298 7972-7978 NN denotes number
T3297 7968-7971 DT denotes the
T3296 7958-7959 NN denotes n
T3295 7956-7957 : denotes ;
T3294 7951-7952 NN denotes ±
T3293 7953-7956 NN denotes sem
T3292 7946-7950 NN denotes mean
T3291 7943-7945 IN denotes as
T3290 7960-7967 VBZ denotes denotes
T3289 7929-7932 VBP denotes are
T3288 7933-7942 VBN denotes presented
T3287 7924-7928 NNS denotes Data
T3286 7923-7994 sentence denotes Data are presented as mean ± sem; n denotes the number of experiments.
T3285 7922-7923 . denotes .
T3284 7921-7922 -RRB- denotes )
T3283 7919-7921 NNP denotes MA
T3282 7917-7919 , denotes ,
T3281 7910-7917 NNP denotes Waltham
T3280 7908-7910 , denotes ,
T3279 7898-7908 NNP denotes Biomedical
T3278 7893-7897 NNP denotes Nova
T3277 7891-7893 , denotes ,
T3276 7882-7889 NNP denotes Profile
T3275 7877-7881 NNP denotes Stat
T3274 7890-7891 NNP denotes M
T3273 7876-7877 -LRB- denotes (
T3272 7861-7866 NN denotes blood
T3271 7867-7875 NN denotes analyzer
T3270 7859-7860 DT denotes a
T3269 7853-7858 VBG denotes using
T3268 7839-7843 VBD denotes were
T3267 7844-7852 VBN denotes obtained
T3266 7822-7823 SYM denotes +
T3265 7821-7822 NN denotes K
T3264 7824-7838 NNS denotes concentrations
T3263 7814-7820 NN denotes Plasma
T3262 7813-7923 sentence denotes Plasma K+ concentrations were obtained using a blood analyzer (Stat Profile M, Nova Biomedical, Waltham, MA).
T3261 7812-7813 . denotes .
T3260 7805-7812 NN denotes cochlea
T3259 7801-7804 DT denotes the
T3258 7796-7800 IN denotes from
T3257 7785-7795 NNS denotes electrodes
T3256 7781-7784 DT denotes the
T3255 7778-7780 IN denotes of
T3254 7767-7777 NN denotes withdrawal
T3253 7761-7766 IN denotes after
T3252 7749-7760 RB denotes immediately
T3251 7739-7743 VBD denotes were
T3250 7744-7748 VBN denotes made
T3249 7726-7738 NNS denotes Calibrations
T3248 7725-7813 sentence denotes Calibrations were made immediately after withdrawal of the electrodes from the cochlea.
T3247 7724-7725 . denotes .
T3246 7709-7710 SYM denotes +
T3245 7711-7724 NN denotes concentration
T3244 7707-7709 NN denotes Na
T3243 7702-7703 -RRB- denotes ]
T3242 7701-7702 SYM denotes +
T3241 7704-7706 VBZ denotes is
T3240 7699-7701 NN denotes Na
T3239 7698-7699 -LRB- denotes [
T3238 7694-7697 CC denotes and
T3237 7692-7694 , denotes ,
T3236 7681-7692 NN denotes selectivity
T3235 7678-7680 VBZ denotes is
T3234 7676-7677 NN denotes A
T3233 7674-7676 , denotes ,
T3232 7669-7674 NN denotes slope
T3231 7666-7668 VBZ denotes is
T3230 7664-7665 NN denotes S
T3229 7662-7664 , denotes ,
T3228 7651-7657 JJ denotes offset
T3227 7658-7662 NN denotes term
T3226 7648-7650 DT denotes an
T3225 7642-7644 NN denotes Vi
T3224 7645-7647 VBZ denotes is
T3223 7636-7641 WRB denotes where
T3222 7634-7636 , denotes ,
T3221 7633-7634 -RRB- denotes )
T3220 7632-7633 -RRB- denotes ]
T3219 7631-7632 SYM denotes +
T3218 7628-7629 -LRB- denotes [
T3217 7626-7627 SYM denotes ×
T3216 7629-7631 NN denotes Na
T3215 7624-7625 NN denotes A
T3214 7622-7623 SYM denotes +
T3213 7620-7621 -RRB- denotes ]
T3212 7619-7620 SYM denotes +
T3211 7617-7618 -LRB- denotes [
T3210 7616-7617 -LRB- denotes (
T3209 7612-7615 NN denotes log
T3208 7610-7611 SYM denotes ×
T3207 7608-7609 NN denotes S
T3206 7606-7607 SYM denotes +
T3205 7603-7605 NN denotes Vi
T3204 7601-7602 SYM denotes =
T3203 7618-7619 NN denotes K
T3202 7599-7600 NN denotes V
T3201 7597-7599 : denotes :
T3200 7596-7597 -RRB- denotes )
T3199 7594-7596 NNP denotes MA
T3198 7592-7594 , denotes ,
T3197 7581-7592 NNP denotes Northampton
T3196 7579-7581 , denotes ,
T3195 7570-7579 NNP denotes OriginLab
T3194 7569-7570 -LRB- denotes (
T3193 7552-7559 VBG denotes fitting
T3192 7551-7552 HYPH denotes -
T3191 7546-7551 NN denotes curve
T3190 7560-7568 NN denotes software
T3189 7536-7545 JJ denotes nonlinear
T3188 7530-7535 VBG denotes using
T3187 7512-7520 NNP denotes Nicolski
T3186 7521-7529 NN denotes equation
T3185 7508-7511 DT denotes the
T3184 7505-7507 IN denotes by
T3183 7490-7497 RB denotes closely
T3182 7486-7489 VBD denotes was
T3181 7498-7504 VBN denotes fitted
T3180 7480-7485 WDT denotes which
T3179 7478-7480 , denotes ,
T3178 7461-7472 NN denotes calibration
T3177 7473-7478 NN denotes curve
T3176 7457-7460 DT denotes the
T3175 7454-7456 IN denotes in
T3174 7441-7453 NN denotes nonlinearity
T3173 7439-7440 DT denotes a
T3172 7428-7429 SYM denotes +
T3171 7426-7428 NN denotes Na
T3170 7422-7425 IN denotes for
T3169 7409-7410 SYM denotes +
T3168 7408-7409 NN denotes K
T3167 7411-7421 NNS denotes electrodes
T3166 7404-7407 DT denotes the
T3165 7401-7403 IN denotes of
T3164 7430-7438 VBD denotes produced
T3163 7383-7388 JJ denotes minor
T3162 7389-7400 NN denotes selectivity
T3161 7379-7382 DT denotes The
T3160 7378-7725 sentence denotes The minor selectivity of the K+ electrodes for Na+ produced a nonlinearity in the calibration curve, which was closely fitted by the Nicolski equation using nonlinear curve-fitting software (OriginLab, Northampton, MA): V = Vi + S × log ([K+] + A × [Na+]), where Vi is an offset term, S is slope, A is selectivity, and [Na+] is Na+ concentration.
T3159 7377-7378 . denotes .
T3158 7375-7377 NNS denotes mM
T3157 7371-7374 CD denotes 150
T3156 7368-7370 IN denotes of
T3155 7352-7353 -RRB- denotes )
T3154 7351-7352 SYM denotes +
T3153 7349-7351 NN denotes Na
T3152 7345-7348 CC denotes and
T3151 7343-7344 SYM denotes +
T3150 7342-7343 NN denotes K
T3149 7341-7342 -LRB- denotes (
T3148 7334-7340 NN denotes cation
T3147 7354-7367 NN denotes concentration
T3146 7325-7333 JJ denotes constant
T3145 7322-7324 IN denotes of
T3144 7312-7321 NNS denotes solutions
T3143 7309-7311 IN denotes in
T3142 7293-7297 VBD denotes were
T3141 7298-7308 VBN denotes calibrated
T3140 7282-7292 NNS denotes electrodes
T3139 7271-7272 HYPH denotes -
T3138 7270-7271 SYM denotes +
T3137 7272-7281 JJ denotes selective
T3136 7269-7270 NN denotes K
T3135 7268-7378 sentence denotes K+-selective electrodes were calibrated in solutions of constant cation (K+ and Na+) concentration of 150 mM.
T3134 7267-7268 . denotes .
T3133 7266-7267 -RRB- denotes )
T3132 7261-7263 CD denotes 30
T3131 7260-7261 SYM denotes ~
T3130 7264-7266 NNS denotes μm
T3129 7259-7260 -LRB- denotes (
T3128 7248-7253 JJ denotes small
T3127 7254-7258 NN denotes hole
T3126 7246-7247 DT denotes a
T3125 7238-7245 VBG denotes picking
T3124 7234-7237 CC denotes and
T3123 7217-7222 NN denotes stria
T3122 7223-7233 NN denotes vascularis
T3121 7213-7216 DT denotes the
T3120 7208-7212 IN denotes over
T3119 7203-7207 NN denotes bone
T3118 7199-7202 DT denotes the
T3117 7190-7198 VBG denotes thinning
T3116 7184-7189 IN denotes after
T3115 7172-7178 JJ denotes apical
T3114 7179-7183 NN denotes turn
T3113 7168-7171 DT denotes the
T3112 7165-7167 IN denotes in
T3111 7161-7164 CC denotes and
T3110 7144-7151 JJ denotes basilar
T3109 7152-7160 NN denotes membrane
T3108 7140-7143 DT denotes the
T3107 7132-7139 IN denotes through
T3106 7115-7116 HYPH denotes -
T3105 7116-7122 NN denotes window
T3104 7110-7115 JJ denotes round
T3103 7123-7131 NN denotes approach
T3102 7108-7109 DT denotes a
T3101 7105-7107 IN denotes by
T3100 7094-7099 JJ denotes basal
T3099 7100-7104 NN denotes turn
T3098 7090-7093 DT denotes the
T3097 7087-7089 IN denotes in
T3096 7077-7081 VBD denotes were
T3095 7082-7086 VBN denotes made
T3094 7064-7076 NNS denotes Measurements
T3093 7063-7268 sentence denotes Measurements were made in the basal turn by a round-window approach through the basilar membrane and in the apical turn after thinning the bone over the stria vascularis and picking a small hole (~30 μm).
T3092 7062-7063 . denotes .
T3091 7061-7062 -RRB- denotes ]
T3090 7059-7061 CD denotes 17
T3089 7058-7059 -LRB- denotes [
T3088 7042-7057 NNS denotes microelectrodes
T3087 7032-7033 HYPH denotes -
T3086 7033-7041 JJ denotes barreled
T3085 7026-7032 JJ denotes double
T2990 6839-6840 . denotes .
T2989 6830-6839 NN denotes electrode
T2988 6826-6829 DT denotes the
T2987 6823-6825 IN denotes at
T2986 6815-6822 NN denotes density
T2985 6807-6814 JJ denotes current
T2984 6804-6806 IN denotes as
T2983 6790-6793 VBD denotes was
T2982 6794-6803 VBN denotes expressed
T2981 6783-6789 NN denotes output
T2980 6779-6782 DT denotes The
T2979 6778-6840 sentence denotes The output was expressed as current density at the electrode.
T2978 6777-6778 . denotes .
T2977 6775-6777 NN denotes Hz
T2976 6771-6774 CD denotes 0.5
T2975 6768-6770 IN denotes of
T2974 6763-6767 NN denotes rate
T2973 6761-6762 DT denotes a
T2972 6758-6760 IN denotes at
T2971 6756-6757 -RRB- denotes )
T2970 6750-6752 CD denotes 16
T2969 6753-6756 NN denotes bit
T2968 6749-6750 -LRB- denotes (
T2967 6734-6738 VBD denotes were
T2966 6713-6714 HYPH denotes -
T2965 6714-6723 JJ denotes sensitive
T2964 6708-6713 NN denotes phase
T2963 6724-6733 NNS denotes detectors
T2962 6704-6707 DT denotes the
T2961 6699-6703 IN denotes from
T2960 6739-6748 VBN denotes digitized
T2959 6691-6698 NNS denotes signals
T2958 6687-6690 DT denotes The
T2957 6686-6778 sentence denotes The signals from the phase-sensitive detectors were digitized (16 bit) at a rate of 0.5 Hz.
T2956 6685-6686 . denotes .
T2955 6673-6677 NN denotes bath
T2954 6678-6685 NN denotes chamber
T2953 6669-6672 DT denotes the
T2952 6666-6668 IN denotes in
T2951 6656-6665 NN denotes reference
T2950 6653-6655 IN denotes as
T2949 6644-6645 -RRB- denotes )
T2948 6638-6639 HYPH denotes -
T2947 6636-6638 CD denotes 26
T2946 6639-6644 NN denotes gauge
T2945 6635-6636 -LRB- denotes (
T2944 6646-6652 VBD denotes served
T2943 6619-6624 JJ denotes black
T2942 6618-6619 HYPH denotes -
T2941 6616-6618 NN denotes Pt
T2940 6625-6634 NN denotes electrode
T2939 6614-6615 DT denotes A
T2938 6613-6686 sentence denotes A Pt-black electrode (26-gauge) served as reference in the bath chamber.
T2937 6612-6613 . denotes .
T2936 6611-6612 -RRB- denotes )
T2935 6609-6611 NNP denotes MA
T2934 6607-6609 , denotes ,
T2933 6599-6607 NNP denotes Falmouth
T2932 6594-6598 NNP denotes East
T2931 6592-6594 , denotes ,
T2930 6587-6592 NNP denotes Wares
T2929 6579-6586 NNP denotes Science
T2928 6577-6579 , denotes ,
T2927 6573-6577 CD denotes 1.05
T2926 6560-6564 NN denotes ASET
T2925 6558-6559 : denotes ;
T2924 6556-6558 NNP denotes MA
T2923 6554-6556 , denotes ,
T2922 6550-6554 NNP denotes Dale
T2921 6543-6549 NNP denotes Forest
T2920 6541-6543 , denotes ,
T2919 6530-6541 NNP denotes Electronics
T2918 6519-6529 NNP denotes Applicable
T2917 6565-6572 NN denotes version
T2916 6518-6519 -LRB- denotes (
T2915 6501-6508 NN denotes bimorph
T2914 6509-6517 NNS denotes elements
T2913 6491-6492 HYPH denotes -
T2912 6492-6500 JJ denotes electric
T2911 6486-6491 JJ denotes piezo
T2910 6483-6485 IN denotes by
T2909 6480-6482 NN denotes Hz
T2908 6475-6476 HYPH denotes –
T2907 6476-6479 CD denotes 400
T2906 6472-6475 CD denotes 200
T2905 6469-6471 IN denotes at
T2904 6460-6468 VBN denotes vibrated
T2903 6456-6459 CC denotes and
T2902 6445-6455 NN denotes epithelium
T2901 6441-6444 DT denotes the
T2900 6438-6440 IN denotes of
T2899 6423-6429 JJ denotes apical
T2898 6430-6437 NN denotes surface
T2897 6419-6422 DT denotes the
T2896 6414-6418 IN denotes from
T2895 6411-6413 NN denotes μm
T2894 6407-6408 HYPH denotes –
T2893 6408-6410 CD denotes 30
T2892 6405-6407 CD denotes 20
T2891 6390-6393 VBD denotes was
T2890 6380-6385 JJ denotes black
T2889 6379-6380 HYPH denotes -
T2888 6377-6379 NN denotes Pt
T2887 6386-6389 NN denotes tip
T2886 6375-6376 DT denotes a
T2885 6370-6374 IN denotes with
T2884 6394-6404 VBN denotes positioned
T2883 6350-6354 NN denotes wire
T2882 6346-6347 HYPH denotes -
T2881 6347-6349 NN denotes Ir
T2880 6344-6346 NN denotes Pt
T2879 6355-6369 NN denotes microelectrode
T2878 6342-6343 DT denotes A
T2877 6341-6613 sentence denotes A Pt-Ir wire microelectrode with a Pt-black tip was positioned 20–30 μm from the apical surface of the epithelium and vibrated at 200–400 Hz by piezo-electric bimorph elements (Applicable Electronics, Forest Dale, MA; ASET version 1.05, Science Wares, East Falmouth, MA).
T2876 6340-6341 . denotes .
T2875 6339-6340 -RRB- denotes ]
T2874 6337-6339 CD denotes 16
T2873 6336-6337 -LRB- denotes [
T2872 6322-6326 VBD denotes were
T2871 6302-6310 JJ denotes marginal
T2870 6296-6301 NN denotes stria
T2869 6311-6321 NN denotes epithelium
T2868 6292-6295 DT denotes the
T2867 6289-6291 IN denotes by
T2866 6279-6288 VBN denotes generated
T2865 6327-6335 VBN denotes recorded
T2864 6270-6278 NNS denotes Currents
T2863 6269-6341 sentence denotes Currents generated by the stria marginal epithelium were recorded [16].
T2862 6268-6269 . denotes .
T2861 6253-6268 NN denotes microdissection
T2860 6250-6252 IN denotes by
T2859 6237-6240 VBD denotes was
T2858 6228-6236 NN denotes ligament
T2857 6221-6227 JJ denotes spiral
T2856 6213-6220 IN denotes without
T2855 6241-6249 VBN denotes obtained
T2854 6202-6212 NN denotes vascularis
T2853 6196-6201 NN denotes Stria
T2852 6195-6269 sentence denotes Stria vascularis without spiral ligament was obtained by microdissection.
T2851 6194-6195 . denotes .
T2850 6193-6194 -RRB- denotes )
T2849 6189-6193 RB denotes i.p.
T2848 6186-6188 NN denotes kg
T2847 6185-6186 SYM denotes /
T2846 6179-6182 CD denotes 100
T2845 6183-6185 NNS denotes mg
T2844 6178-6179 -LRB- denotes (
T2843 6164-6177 NN denotes pentobarbital
T2842 6157-6163 NN denotes sodium
T2841 6152-6156 IN denotes with
T2840 6132-6138 RB denotes deeply
T2839 6127-6131 VBD denotes were
T2838 6139-6151 VBN denotes anesthetized
T2837 6119-6126 NNS denotes Animals
T2836 6118-6195 sentence denotes Animals were deeply anesthetized with sodium pentobarbital (100 mg/kg i.p.).
T2835 6101-6118 NN denotes electrophysiology
T2834 6095-6100 FW denotes vitro
T2833 6092-6094 FW denotes In
T2809 6089-6090 . denotes .
T2808 6082-6084 NN denotes RT
T2807 6085-6089 NN denotes step
T2806 6078-6081 DT denotes the
T3311 8048-8049 . denotes .
T3310 8042-8043 SYM denotes <
T3309 8040-8041 NN denotes p
T3308 8044-8048 CD denotes 0.05
T3307 8035-8039 WRB denotes when
T3306 8023-8034 JJ denotes significant
T3305 8007-8011 VBD denotes were
T3304 8012-8022 VBN denotes considered
T3303 7995-8006 NNS denotes Differences
T3302 7994-8049 sentence denotes Differences were considered significant when p < 0.05.
T3301 7993-7994 . denotes .
T2805 6075-6077 IN denotes of
T2804 6066-6074 NN denotes omission
T2803 6063-6065 IN denotes by
T2802 6057-6061 CD denotes 0.02
T2801 6061-6062 NN denotes %
T2800 6056-6057 SYM denotes <
T2799 6053-6055 VB denotes be
T2798 6050-6052 TO denotes to
T2797 6037-6040 VBD denotes was
T2796 6029-6036 NNS denotes samples
T2795 6025-6028 NN denotes ear
T2794 6019-6024 JJ denotes inner
T2793 6016-6018 IN denotes of
T2792 6041-6049 VBN denotes assessed
T2791 6002-6015 NN denotes contamination
T2790 5994-6001 JJ denotes Genomic
T2789 5993-6090 sentence denotes Genomic contamination of inner ear samples was assessed to be <0.02% by omission of the RT step.
T2788 5992-5993 . denotes .
T2787 5985-5992 NNS denotes amounts
T2786 5978-5984 NN denotes tissue
T2785 5970-5977 VB denotes compare
T2784 5967-5969 TO denotes to
T2783 5957-5961 VBD denotes were
T2782 5952-5956 NN denotes rRNA
T2781 5948-5951 NN denotes 18S
T2780 5945-5947 IN denotes of
T2740 5774-5775 SYM denotes ×
T2739 5779-5780 CD denotes 4
T2738 5769-5773 CD denotes 3.39
T2737 5767-5768 SYM denotes +
T2736 5762-5763 SYM denotes *
T2735 5764-5766 NN denotes S4
T2734 5759-5760 HYPH denotes -
T2733 5757-5759 CD denotes 10
T2732 5755-5756 SYM denotes ×
T2731 5760-5761 CD denotes 3
T2730 5749-5754 CD denotes 11.38
T2729 5747-5748 SYM denotes +
T2728 5742-5743 SYM denotes ×
T2727 5744-5746 NN denotes S3
T2726 5737-5741 CD denotes 0.15
T2725 5735-5736 SYM denotes +
T2724 5730-5731 SYM denotes ×
T2723 5732-5734 NN denotes S2
T2722 5725-5729 CD denotes 1.05
T2721 5723-5724 SYM denotes +
T2720 5719-5720 SYM denotes ×
T2719 5721-5722 NN denotes S
T2718 5714-5718 CD denotes 3.80
T2717 5712-5713 SYM denotes +
T2716 5705-5706 SYM denotes =
T2715 5707-5711 CD denotes 7.39
T2714 5703-5704 NN denotes F
T2713 5701-5703 : denotes :
T2712 5684-5685 HYPH denotes -
T2711 5685-5690 NN denotes order
T2710 5679-5684 JJ denotes fifth
T2709 5674-5675 HYPH denotes -
T2708 5675-5678 NN denotes fit
T2707 5670-5674 JJ denotes best
T2706 5691-5701 NN denotes polynomial
T2705 5668-5669 DT denotes a
T2704 5664-5667 IN denotes via
T2703 5651-5657 NN denotes growth
T2702 5658-5663 NN denotes curve
T2701 5647-5650 DT denotes the
T2700 5644-5646 IN denotes of
T2699 5630-5631 SYM denotes -
T2698 5631-5637 JJ denotes linear
T2697 5627-5630 NN denotes log
T2696 5638-5643 NN denotes phase
T2695 5623-5626 DT denotes the
T2694 5620-5622 IN denotes of
T2693 5618-5619 -RRB- denotes )
T2692 5617-5618 NN denotes S
T2691 5616-5617 -LRB- denotes (
T2690 5610-5615 NN denotes slope
T2689 5606-5609 DT denotes the
T2688 5601-5605 IN denotes from
T2687 5588-5591 VBD denotes was
T2686 5586-5587 -RRB- denotes )
T2685 5585-5586 NN denotes F
T2684 5584-5585 -LRB- denotes (
T2683 5592-5600 VBN denotes obtained
T2682 5575-5583 NN denotes Fidelity
T2681 5574-5786 sentence denotes Fidelity (F) was obtained from the slope (S) of the log-linear phase of the growth curve via a best-fit fifth-order polynomial: F = 7.39 + 3.80 × S + 1.05 × S2 + 0.15 × S3 + 11.38 × 10-3 * S4 + 3.39 × 10-4 × S5.
T2680 5573-5574 . denotes .
T2679 5572-5573 -RRB- denotes ]
T2678 5570-5572 CD denotes 15
T2677 5569-5570 -LRB- denotes [
T2676 5552-5558 VBN denotes chosen
T2675 5559-5568 NN denotes threshold
T2674 5550-5551 DT denotes a
T2673 5532-5541 NNS denotes molecules
T2672 5524-5531 NN denotes product
T2671 5521-5523 IN denotes of
T2670 5514-5520 NN denotes number
T2669 5510-5513 DT denotes the
T2668 5504-5509 WDT denotes which
T2667 5542-5549 VBZ denotes reaches
T2666 5501-5503 IN denotes at
T2665 5495-5500 NN denotes cycle
T2664 5491-5494 DT denotes the
T2663 5488-5490 VBZ denotes is
T2662 5485-5487 NN denotes Ct
T2661 5481-5484 CC denotes and
T2660 5472-5480 NN denotes reaction
T2659 5468-5471 DT denotes the
T2658 5465-5467 IN denotes of
T2657 5456-5464 NN denotes fidelity
T2656 5452-5455 DT denotes the
T2655 5449-5451 VBZ denotes is
T2654 5447-5448 NN denotes F
T2653 5445-5447 , denotes ,
T2652 5436-5445 NNS denotes molecules
T2651 5428-5435 NN denotes product
T2650 5425-5427 IN denotes of
T2649 5418-5424 NN denotes number
T2648 5414-5417 DT denotes the
T2647 5409-5410 NN denotes P
T2646 5411-5413 VBZ denotes is
T2645 5403-5408 WRB denotes where
T2644 5401-5402 -RRB- denotes )
T2643 5398-5399 SYM denotes ^
T2642 5396-5397 NN denotes F
T2641 5395-5396 -LRB- denotes (
T2640 5399-5401 NN denotes Ct
T2639 5393-5394 SYM denotes /
T2638 5389-5390 SYM denotes =
T2637 5391-5392 NN denotes P
T2636 5387-5388 NN denotes T
T2635 5384-5386 IN denotes to
T2634 5374-5383 VBG denotes according
T2633 5358-5362 VBD denotes were
T2632 5356-5357 -RRB- denotes )
T2631 5355-5356 NN denotes T
T2630 5354-5355 -LRB- denotes (
T2629 5363-5373 VBN denotes quantified
T2628 5344-5353 NNS denotes molecules
T2626 4736-5574 sentence denotes Table 1 Primers Template Primers Product Length Melting Temperature Hot Measurement Fidelity n 18S gag gtt cga aga cga tca ga (sense) 316 bp 83.2 ± 02°C 78°C 1.89 ± 0.02 17 tcg ctc cac caa cta aga ac (antisense) KCNJ10 tgg tgt ggt gtg gta tct gg (sense) 411 bp 83.2 ± 02°C 78°C 1.86 ± 0.02 15 tga agc agt ttg cct gtc ac (antisense) KCNQ1 ttt gtt cat ccc cat ctc ag (sense) 239 bp 82.5 ± 02°C 79°C 1.85 ± 0.02 17 gtt gct ggg tag gaa gag (antisense) KCNQ4 ccc gga aac cct tct gtg tc (sense) 245 bp 83.2 ± 02°C 80°C 1.87 ± 0.01 17 aaa gat gag cac cag gaa cc (antisense) Template molecules (T) were quantified according to T = P / (F ^Ct) where P is the number of product molecules, F is the fidelity of the reaction and Ct is the cycle at which the number of product molecules reaches a chosen threshold [15].
T3084 7021-7025 IN denotes with
T3083 7007-7011 VBD denotes were
T3082 7005-7006 -RRB- denotes ]
T3081 7004-7005 SYM denotes +
T3080 7002-7003 -LRB- denotes [
T3079 6988-7001 JJ denotes endolymphatic
T3078 7003-7004 NN denotes K
T3077 6984-6987 DT denotes the
T3076 6980-6983 CC denotes and
T3075 7012-7020 VBN denotes measured
T3074 6957-6969 JJ denotes endocochlear
T3073 6970-6979 NN denotes potential
T3072 6953-6956 DT denotes The
T3071 6952-7063 sentence denotes The endocochlear potential and the endolymphatic [K+] were measured with double-barreled microelectrodes [17].
T3070 6951-6952 . denotes .
T3069 6950-6951 -RRB- denotes )
T3068 6948-6950 RB denotes ip
T3067 6945-6947 NN denotes kg
T3066 6944-6945 SYM denotes /
T3065 6938-6941 CD denotes 140
T3064 6936-6938 , denotes ,
T3063 6925-6931 NN denotes sodium
T3062 6932-6936 NN denotes salt
T3061 6908-6924 NN denotes thiobutabarbital
T3060 6942-6944 NNS denotes mg
T3059 6907-6908 -LRB- denotes (
T3058 6899-6906 NN denotes inactin
T3057 6894-6898 IN denotes with
T3056 6876-6880 VBD denotes were
T3055 6881-6893 VBN denotes anesthetized
T3054 6868-6875 NNS denotes Animals
T3053 6867-6952 sentence denotes Animals were anesthetized with inactin (thiobutabarbital sodium salt, 140 mg/kg ip).
T3052 6850-6867 NN denotes electrophysiology
T3051 6845-6849 FW denotes situ
T3050 6842-6844 FW denotes In
T2779 5962-5966 VBN denotes used
T2778 5929-5944 NNS denotes Quantifications
T2777 5928-5993 sentence denotes Quantifications of 18S rRNA were used to compare tissue amounts.
T2776 5927-5928 . denotes .
T2775 5924-5925 SYM denotes ^
T2774 5925-5927 NN denotes Ct
T2773 5922-5923 NN denotes F
T2772 5920-5921 SYM denotes ×
T2771 5916-5917 SYM denotes =
T2770 5918-5919 NN denotes T
T2769 5912-5915 NN denotes PCt
T2768 5909-5911 IN denotes to
T2767 5899-5908 VBG denotes according
T2766 5894-5898 NN denotes rRNA
T2765 5890-5893 NN denotes 18S
T2764 5887-5889 IN denotes of
T2763 5879-5886 NNS denotes amounts
T2762 5873-5878 VBN denotes known
T2761 5870-5872 IN denotes of
T2760 5856-5869 NN denotes amplification
T2759 5853-5855 IN denotes by
T2758 5838-5841 VBD denotes was
T2757 5836-5837 -RRB- denotes )
T2756 5833-5836 NN denotes PCt
T2755 5832-5833 -LRB- denotes (
T2754 5822-5831 NN denotes threshold
T2753 5819-5821 IN denotes at
T2752 5809-5818 NNS denotes molecules
T2751 5801-5808 NN denotes product
T2750 5798-5800 IN denotes of
T2749 5842-5852 VBN denotes determined
T2748 5791-5797 NN denotes number
T2747 5787-5790 DT denotes The
T2746 5786-5928 sentence denotes The number of product molecules at threshold (PCt) was determined by amplification of known amounts of 18S rRNA according to PCt = T × F ^Ct.
T2745 5785-5786 . denotes .
T2744 5781-5782 SYM denotes ×
T2743 5783-5785 NN denotes S5
T2742 5778-5779 HYPH denotes -
T2741 5776-5778 CD denotes 10
T1317 98-106 VBN denotes measured
T1320 78-92 NNS denotes concentrations
T1322 57-60 CC denotes and
T1323 61-74 JJ denotes perilymphatic
T1324 75-76 NN denotes K
T1325 76-77 SYM denotes +
T1326 93-97 VBD denotes were
T1327 107-109 IN denotes in
T1328 110-115 JJ denotes young
T1329 122-126 NNS denotes mice
T1330 116-121 JJ denotes adult
T1331 127-128 -LRB- denotes (
T1332 132-137 NN denotes month
T1333 128-129 CD denotes 1
T1334 130-131 CD denotes 4
T1335 129-130 SYM denotes –
T1336 138-140 IN denotes of
T1337 141-144 NN denotes age
T1338 144-145 -RRB- denotes )
T1339 146-150 WDT denotes that
T1340 158-162 VBP denotes have
T1341 151-157 CC denotes either
T1342 163-164 -LRB- denotes (
T1343 164-171 NN denotes Slc26a4
T1344 171-172 SYM denotes +
T1345 172-173 HYPH denotes /
T1346 173-174 SYM denotes +
T1347 174-175 -RRB- denotes )
T1348 176-178 CC denotes or
T1349 179-183 VBP denotes lack
T1350 184-185 -LRB- denotes (
T1351 185-192 NN denotes Slc26a4
T1352 192-193 SYM denotes -
T1353 193-194 HYPH denotes /
T1354 194-195 SYM denotes -
T1355 195-196 -RRB- denotes )
T1356 197-198 DT denotes a
T1357 210-214 NN denotes gene
T1358 199-209 JJ denotes functional
T1359 215-218 IN denotes for
T1360 219-226 NN denotes pendrin
T1361 227-228 -LRB- denotes [
T1362 228-230 CD denotes 14
T1363 230-231 -RRB- denotes ]
T1364 231-232 . denotes .
T1365 232-450 sentence denotes The mouse strain 129Sv/Ev (Taconic, Germantown, NY) was chosen as the source of Slc26a4+/+ controls, since Slc26a4-/- mice were propagated in the this strain and generated using a stem cell line derived from 129Sv/Ev.
T1366 233-236 DT denotes The
T1367 243-249 NN denotes strain
T1368 237-242 NN denotes mouse
T1369 289-295 VBN denotes chosen
T1370 250-255 NN denotes 129Sv
T1371 256-258 NN denotes Ev
T1372 255-256 HYPH denotes /
T1373 259-260 -LRB- denotes (
T1374 260-267 NNP denotes Taconic
T1375 267-269 , denotes ,
T1376 269-279 NNP denotes Germantown
T1377 279-281 , denotes ,
T1378 281-283 NNP denotes NY
T1379 283-284 -RRB- denotes )
T1380 285-288 VBD denotes was
T1381 296-298 IN denotes as
T1382 299-302 DT denotes the
T1383 303-309 NN denotes source
T1384 310-312 IN denotes of
T1385 313-320 NN denotes Slc26a4
T1386 324-332 NNS denotes controls
T1387 320-321 SYM denotes +
T1388 321-322 HYPH denotes /
T1389 322-323 SYM denotes +
T1390 332-334 , denotes ,
T1391 334-339 IN denotes since
T1392 361-371 VBN denotes propagated
T1393 340-347 NN denotes Slc26a4
T1394 351-355 NNS denotes mice
T1395 347-348 SYM denotes -
T1396 348-349 HYPH denotes /
T1397 349-350 SYM denotes -
T1398 356-360 VBD denotes were
T1399 372-374 IN denotes in
T1400 375-378 DT denotes the
T1401 384-390 NN denotes strain
T1402 379-383 DT denotes this
T1403 391-394 CC denotes and
T1404 395-404 VBN denotes generated
T1405 405-410 VBG denotes using
T1406 411-412 DT denotes a
T1407 423-427 NN denotes line
T1408 413-417 NN denotes stem
T1409 418-422 NN denotes cell
T1410 428-435 VBN denotes derived
T1411 436-440 IN denotes from
T1412 441-446 NN denotes 129Sv
T1413 447-449 NN denotes Ev
T1414 446-447 HYPH denotes /
T1415 449-450 . denotes .
T1416 451-489 sentence denotes Slc26a4+/+ and Slc26a4-/- were agouti.
T1417 451-458 NN denotes Slc26a4
T1418 477-481 VBD denotes were
T1419 458-459 SYM denotes +
T1420 459-460 HYPH denotes /
T1421 460-461 SYM denotes +
T1422 462-465 CC denotes and
T1423 466-473 NN denotes Slc26a4
T1424 473-474 SYM denotes -
T1425 474-475 HYPH denotes /
T1426 475-476 SYM denotes -
T1427 482-488 NN denotes agouti
T1428 488-489 . denotes .
T1429 489-524 sentence denotes They did not differ in coat color.
T1430 490-494 PRP denotes They
T1431 503-509 VB denotes differ
T1432 495-498 VBD denotes did
T1433 499-502 RB denotes not
T1434 510-512 IN denotes in
T1435 513-517 NN denotes coat
T1436 518-523 NN denotes color
T1437 523-524 . denotes .
T1438 524-649 sentence denotes Differences in pigmentation were verified using coisogenic age-matched Slc26a4+/+ and Slc26a4-/- that were bred in parallel.
T1439 525-536 NNS denotes Differences
T1440 558-566 VBN denotes verified
T1441 537-539 IN denotes in
T1442 540-552 NN denotes pigmentation
T1443 553-557 VBD denotes were
T1444 567-572 VBG denotes using
T1445 573-583 JJ denotes coisogenic
T1446 596-603 NN denotes Slc26a4
T1447 584-587 NN denotes age
T1448 588-595 VBN denotes matched
T1449 587-588 HYPH denotes -
T1450 603-604 SYM denotes +
T1451 604-605 HYPH denotes /
T1452 605-606 SYM denotes +
T1453 607-610 CC denotes and
T1454 611-618 NN denotes Slc26a4
T1455 618-619 SYM denotes -
T1456 619-620 HYPH denotes /
T1457 620-621 SYM denotes -
T1458 622-626 WDT denotes that
T1459 632-636 VBN denotes bred
T1460 627-631 VBD denotes were
T1461 637-639 IN denotes in
T1462 640-648 NN denotes parallel
T1463 648-649 . denotes .
T1464 650-829 sentence denotes Expression of key proteins involved in the generation of the endocochlear potential and the transport of K+ was studied using confocal immunocytochemistry and quantitative RT-PCR.
T1465 650-660 NN denotes Expression
T1466 762-769 VBN denotes studied
T1467 661-663 IN denotes of
T1468 664-667 JJ denotes key
T1469 668-676 NN denotes proteins
T1470 677-685 VBN denotes involved
T1471 686-688 IN denotes in
T1472 689-692 DT denotes the
T1473 693-703 NN denotes generation
T1474 704-706 IN denotes of
T1475 707-710 DT denotes the
T1476 724-733 NN denotes potential
T1477 711-723 JJ denotes endocochlear
T1478 734-737 CC denotes and
T1479 738-741 DT denotes the
T1480 742-751 NN denotes transport
T1481 752-754 IN denotes of
T1482 755-756 NN denotes K
T1483 756-757 SYM denotes +
T1484 758-761 VBD denotes was
T1485 770-775 VBG denotes using
T1486 776-784 JJ denotes confocal
T1487 785-804 NN denotes immunocytochemistry
T1488 805-808 CC denotes and
T1489 809-821 JJ denotes quantitative
T1490 825-828 NN denotes PCR
T1491 822-824 NN denotes RT
T1492 824-825 HYPH denotes -
T1493 828-829 . denotes .
T1494 829-944 sentence denotes K+ secretion and the generation of the endocochlear potential were measured using electrophysiological techniques.
T1495 830-831 NN denotes K
T1496 833-842 NN denotes secretion
T1497 831-832 SYM denotes +
T1498 897-905 VBN denotes measured
T1499 843-846 CC denotes and
T1500 847-850 DT denotes the
T1501 851-861 NN denotes generation
T1502 862-864 IN denotes of
T1503 865-868 DT denotes the
T1504 882-891 NN denotes potential
T1505 869-881 JJ denotes endocochlear
T1506 892-896 VBD denotes were
T1507 906-911 VBG denotes using
T1508 912-932 JJ denotes electrophysiological
T1509 933-943 NNS denotes techniques
T1510 943-944 . denotes .
T1511 944-1053 sentence denotes All experiments were approved by the Institutional Animal Care and Use Committee of Kansas State University.
T1512 945-948 DT denotes All
T1513 949-960 NNS denotes experiments
T1514 966-974 VBN denotes approved
T1515 961-965 VBD denotes were
T1516 975-977 IN denotes by
T1517 978-981 DT denotes the
T1518 1016-1025 NNP denotes Committee
T1519 982-995 NNP denotes Institutional
T1520 996-1002 NNP denotes Animal
T1521 1003-1007 NNP denotes Care
T1522 1008-1011 CC denotes and
T1523 1012-1015 NNP denotes Use
T1524 1026-1028 IN denotes of
T1525 1029-1035 NNP denotes Kansas
T1526 1042-1052 NNP denotes University
T1527 1036-1041 NNP denotes State
T1528 1052-1053 . denotes .
T1679 1064-1083 NN denotes immunocytochemistry
T1680 1083-1276 sentence denotes Animals were deeply anesthetized with sodium pentobarbital (100 mg/kg i.p.) and transcardially perfused with a Cl--free phosphate-buffered Na-gluconate solution containing 4% paraformaldehyde.
T1681 1084-1091 NNS denotes Animals
T1682 1104-1116 VBN denotes anesthetized
T1683 1092-1096 VBD denotes were
T1684 1097-1103 RB denotes deeply
T1685 1117-1121 IN denotes with
T1686 1122-1128 NN denotes sodium
T1687 1129-1142 NN denotes pentobarbital
T1688 1143-1144 -LRB- denotes (
T1689 1148-1150 NNS denotes mg
T1690 1144-1147 CD denotes 100
T1691 1150-1151 SYM denotes /
T1692 1151-1153 NN denotes kg
T1693 1154-1158 RB denotes i.p.
T1694 1158-1159 -RRB- denotes )
T1695 1160-1163 CC denotes and
T1696 1164-1178 RB denotes transcardially
T1697 1179-1187 VBN denotes perfused
T1698 1188-1192 IN denotes with
T1699 1193-1194 DT denotes a
T1700 1236-1244 NN denotes solution
T1701 1195-1197 NN denotes Cl
T1702 1199-1203 JJ denotes free
T1703 1197-1198 SYM denotes -
T1704 1198-1199 HYPH denotes -
T1705 1204-1213 NN denotes phosphate
T1706 1214-1222 VBN denotes buffered
T1707 1213-1214 HYPH denotes -
T1708 1223-1225 NN denotes Na
T1709 1226-1235 NN denotes gluconate
T1710 1225-1226 SYM denotes -
T1711 1245-1255 VBG denotes containing
T1712 1256-1257 CD denotes 4
T1713 1257-1258 NN denotes %
T1714 1259-1275 NN denotes paraformaldehyde
T1715 1275-1276 . denotes .
T1716 1276-1451 sentence denotes Temporal bones were removed and cochleae fixed by perilymphatic perfusion, decalcified in EDTA, processed through a sucrose gradient and infiltrated with polyethylene glycol.
T1717 1277-1285 JJ denotes Temporal
T1718 1286-1291 NNS denotes bones
T1719 1297-1304 VBN denotes removed
T1720 1292-1296 VBD denotes were
T1721 1305-1308 CC denotes and
T1722 1309-1317 NNS denotes cochleae
T1723 1318-1323 VBN denotes fixed
T1724 1324-1326 IN denotes by
T1725 1327-1340 JJ denotes perilymphatic
T1726 1341-1350 NN denotes perfusion
T1727 1350-1352 , denotes ,
T1728 1352-1363 VBN denotes decalcified
T1729 1364-1366 IN denotes in
T1730 1367-1371 NN denotes EDTA
T1731 1371-1373 , denotes ,
T1732 1373-1382 VBN denotes processed
T1733 1383-1390 IN denotes through
T1734 1391-1392 DT denotes a
T1735 1401-1409 NN denotes gradient
T1736 1393-1400 NN denotes sucrose
T1737 1410-1413 CC denotes and
T1738 1414-1425 VBN denotes infiltrated
T1739 1426-1430 IN denotes with
T1740 1431-1443 NN denotes polyethylene
T1741 1444-1450 NN denotes glycol
T1742 1450-1451 . denotes .
T1743 1451-1598 sentence denotes Mid-modiolar cryosections (12 μm, CM3050S, Leica, Nussloch, Germany) were blocked in PBS with 0.2% Triton-X (PBS-TX) and 10% bovine serum albumin.
T1744 1452-1464 JJ denotes Mid-modiolar
T1745 1465-1477 NNS denotes cryosections
T1746 1526-1533 VBN denotes blocked
T1747 1478-1479 -LRB- denotes (
T1748 1486-1493 NN denotes CM3050S
T1749 1479-1481 CD denotes 12
T1750 1482-1484 NNS denotes μm
T1751 1484-1486 , denotes ,
T1752 1493-1495 , denotes ,
T1753 1495-1500 NNP denotes Leica
T1754 1500-1502 , denotes ,
T1755 1502-1510 NNP denotes Nussloch
T1756 1510-1512 , denotes ,
T1757 1512-1519 NNP denotes Germany
T1758 1519-1520 -RRB- denotes )
T1759 1521-1525 VBD denotes were
T1760 1534-1536 IN denotes in
T1761 1537-1540 NN denotes PBS
T1762 1541-1545 IN denotes with
T1763 1546-1549 CD denotes 0.2
T1764 1549-1550 NN denotes %
T1765 1558-1559 NN denotes X
T1766 1551-1557 NN denotes Triton
T1767 1557-1558 HYPH denotes -
T1768 1560-1561 -LRB- denotes (
T1769 1565-1567 NN denotes TX
T1770 1561-1564 NN denotes PBS
T1771 1564-1565 HYPH denotes -
T1772 1567-1568 -RRB- denotes )
T1773 1569-1572 CC denotes and
T1774 1573-1575 CD denotes 10
T1775 1575-1576 NN denotes %
T1776 1590-1597 NN denotes albumin
T1777 1577-1583 JJ denotes bovine
T1778 1584-1589 NN denotes serum
T1779 1597-1598 . denotes .
T1780 1598-2039 sentence denotes Slides were incubated overnight at 4°C with primary antibodies in PBS-TX with 1–3% BSA [rabbit anti-pendrin, 1:500 (h766–780); rat anti-ZO-1, 1:100 (Chemicon, Temecula, CA); goat anti-KCNQ1, 1:200 (C20, Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-KCNE1, 1:200 (Alomone, Jerusalem, Israel); rabbit anti-KCNJ10, 1:300 (Alomone); rabbit anti-SLC12A2, 1:100 (Chemicon); and rabbit anti-connexin 26, 1:100 (Zymed, San Francisco, CA)].
T1781 1599-1605 NNS denotes Slides
T1782 1611-1620 VBN denotes incubated
T1783 1606-1610 VBD denotes were
T1784 1621-1630 RB denotes overnight
T1785 1631-1633 IN denotes at
T1786 1634-1635 CD denotes 4
T1787 1635-1637 NN denotes °C
T1788 1638-1642 IN denotes with
T1789 1643-1650 JJ denotes primary
T1790 1651-1661 NNS denotes antibodies
T1791 1662-1664 IN denotes in
T1792 1665-1668 NN denotes PBS
T1793 1669-1671 NN denotes TX
T1794 1668-1669 HYPH denotes -
T1795 1672-1676 IN denotes with
T1796 1677-1678 CD denotes 1
T1797 1679-1680 CD denotes 3
T1798 1678-1679 SYM denotes –
T1799 1680-1681 NN denotes %
T1800 1682-1685 NN denotes BSA
T1801 1686-1687 -LRB- denotes [
T1802 1715-1719 NN denotes h766
T1803 1687-1693 NN denotes rabbit
T1804 1694-1706 NN denotes anti-pendrin
T1805 1706-1708 , denotes ,
T1806 1708-1709 CD denotes 1
T1807 1709-1710 SYM denotes :
T1808 1710-1713 CD denotes 500
T1809 1714-1715 -LRB- denotes (
T1810 1719-1720 SYM denotes –
T1811 1720-1723 CD denotes 780
T1812 1723-1724 -RRB- denotes )
T1813 1724-1725 , denotes ;
T1814 1726-1729 NN denotes rat
T1815 1730-1737 NN denotes anti-ZO
T1816 1748-1756 NNP denotes Chemicon
T1817 1737-1738 HYPH denotes -
T1818 1738-1739 CD denotes 1
T1819 1739-1741 , denotes ,
T1820 1741-1742 CD denotes 1
T1821 1742-1743 SYM denotes :
T1822 1743-1746 CD denotes 100
T1823 1747-1748 -LRB- denotes (
T1824 1756-1758 , denotes ,
T1825 1758-1766 NNP denotes Temecula
T1826 1766-1768 , denotes ,
T1827 1768-1770 NNP denotes CA
T1828 1770-1771 -RRB- denotes )
T1829 1771-1772 , denotes ;
T1830 1773-1777 NN denotes goat
T1831 1778-1788 NN denotes anti-KCNQ1
T1832 1797-1800 NN denotes C20
T1833 1788-1790 , denotes ,
T1834 1790-1791 CD denotes 1
T1835 1791-1792 SYM denotes :
T1836 1792-1795 CD denotes 200
T1837 1796-1797 -LRB- denotes (
T1838 1800-1802 , denotes ,
T1839 1802-1807 NNP denotes Santa
T1840 1808-1812 NNP denotes Cruz
T1841 1813-1826 NNP denotes Biotechnology
T1842 1826-1828 , denotes ,
T1843 1828-1833 NNP denotes Santa
T1844 1834-1838 NNP denotes Cruz
T1845 1838-1840 , denotes ,
T1846 1840-1842 NNP denotes CA
T1847 1842-1843 -RRB- denotes )
T1848 1843-1845 , denotes ,
T1849 1845-1851 NN denotes rabbit
T1850 1852-1862 NN denotes anti-KCNE1
T1851 1871-1878 NNP denotes Alomone
T1852 1862-1864 , denotes ,
T1853 1864-1865 CD denotes 1
T1854 1865-1866 SYM denotes :
T1855 1866-1869 CD denotes 200
T1856 1870-1871 -LRB- denotes (
T1857 1878-1880 , denotes ,
T1858 1880-1889 NNP denotes Jerusalem
T1859 1889-1891 , denotes ,
T1860 1891-1897 NNP denotes Israel
T1861 1897-1898 -RRB- denotes )
T1862 1898-1899 , denotes ;
T1863 1900-1906 NN denotes rabbit
T1864 1907-1918 NN denotes anti-KCNJ10
T1865 1927-1934 NNP denotes Alomone
T1866 1918-1920 , denotes ,
T1867 1920-1921 CD denotes 1
T1868 1921-1922 SYM denotes :
T1869 1922-1925 CD denotes 300
T1870 1926-1927 -LRB- denotes (
T1871 1934-1935 -RRB- denotes )
T1872 1935-1936 , denotes ;
T1873 1937-1943 NN denotes rabbit
T1874 1944-1956 NN denotes anti-SLC12A2
T1875 1965-1973 NNP denotes Chemicon
T1876 1956-1958 , denotes ,
T1877 1958-1959 CD denotes 1
T1878 1959-1960 SYM denotes :
T1879 1960-1963 CD denotes 100
T1880 1964-1965 -LRB- denotes (
T1881 1973-1974 -RRB- denotes )
T1882 1974-1975 : denotes ;
T1883 1976-1979 CC denotes and
T1884 1980-1986 NN denotes rabbit
T1885 1987-2000 NN denotes anti-connexin
T1886 2012-2017 NNP denotes Zymed
T1887 2001-2003 CD denotes 26
T1888 2003-2005 , denotes ,
T1889 2005-2006 CD denotes 1
T1890 2006-2007 SYM denotes :
T1891 2007-2010 CD denotes 100
T1892 2011-2012 -LRB- denotes (
T1893 2017-2019 , denotes ,
T1894 2019-2022 NNP denotes San
T1895 2023-2032 NNP denotes Francisco
T1896 2032-2034 , denotes ,
T1897 2034-2036 NNP denotes CA
T1898 2036-2037 -RRB- denotes )
T1899 2037-2038 -RRB- denotes ]
T1900 2038-2039 . denotes .
T1901 2039-2305 sentence denotes Slides were washed in PBS-TX and incubated for 1 h at 25°C with appropriate secondary antibodies at a 1:1000 dilution in PBS-TX with 1–3% BSA [donkey anti-rabbit Alexa 488, chicken anti-rat Alexa 594, and chicken anti-goat Alexa 594 (Molecular Probes, Eugene, OR)].
T1902 2040-2046 NNS denotes Slides
T1903 2052-2058 VBN denotes washed
T1904 2047-2051 VBD denotes were
T1905 2059-2061 IN denotes in
T1906 2062-2065 NN denotes PBS
T1907 2066-2068 NN denotes TX
T1908 2065-2066 HYPH denotes -
T1909 2069-2072 CC denotes and
T1910 2073-2082 VBN denotes incubated
T1911 2083-2086 IN denotes for
T1912 2087-2088 CD denotes 1
T1913 2089-2090 NN denotes h
T1914 2091-2093 IN denotes at
T1915 2094-2096 CD denotes 25
T1916 2096-2098 NN denotes °C
T1917 2099-2103 IN denotes with
T1918 2104-2115 JJ denotes appropriate
T1919 2126-2136 NNS denotes antibodies
T1920 2116-2125 JJ denotes secondary
T1921 2137-2139 IN denotes at
T1922 2140-2141 DT denotes a
T1923 2149-2157 NN denotes dilution
T1924 2142-2143 CD denotes 1
T1925 2143-2144 SYM denotes :
T1926 2144-2148 CD denotes 1000
T1927 2158-2160 IN denotes in
T1928 2161-2164 NN denotes PBS
T1929 2165-2167 NN denotes TX
T1930 2164-2165 HYPH denotes -
T1931 2168-2172 IN denotes with
T1932 2173-2174 CD denotes 1
T1933 2175-2176 CD denotes 3
T1934 2174-2175 SYM denotes –
T1935 2176-2177 NN denotes %
T1936 2178-2181 NN denotes BSA
T1937 2182-2183 -LRB- denotes [
T1938 2284-2290 NNP denotes Probes
T1939 2183-2189 NN denotes donkey
T1940 2202-2207 NNP denotes Alexa
T1941 2190-2201 JJ denotes anti-rabbit
T1942 2208-2211 CD denotes 488
T1943 2211-2213 , denotes ,
T1944 2213-2220 NN denotes chicken
T1945 2230-2235 NNP denotes Alexa
T1946 2221-2229 JJ denotes anti-rat
T1947 2236-2239 CD denotes 594
T1948 2239-2241 , denotes ,
T1949 2241-2244 CC denotes and
T1950 2245-2252 NN denotes chicken
T1951 2263-2268 NNP denotes Alexa
T1952 2253-2262 JJ denotes anti-goat
T1953 2269-2272 CD denotes 594
T1954 2273-2274 -LRB- denotes (
T1955 2274-2283 NNP denotes Molecular
T1956 2290-2292 , denotes ,
T1957 2292-2298 NNP denotes Eugene
T1958 2298-2300 , denotes ,
T1959 2300-2302 NNP denotes OR
T1960 2302-2303 -RRB- denotes )
T1961 2303-2304 -RRB- denotes ]
T1962 2304-2305 . denotes .
T1963 2305-2406 sentence denotes Actin filaments were visualized by staining with Alexa 488 conjugated phalloidin (Molecular Probes).
T1964 2306-2311 NN denotes Actin
T1965 2312-2321 NNS denotes filaments
T1966 2327-2337 VBN denotes visualized
T1967 2322-2326 VBD denotes were
T1968 2338-2340 IN denotes by
T1969 2341-2349 VBG denotes staining
T1970 2350-2354 IN denotes with
T1971 2355-2360 NNP denotes Alexa
T1972 2365-2375 VBN denotes conjugated
T1973 2361-2364 CD denotes 488
T1974 2376-2386 NN denotes phalloidin
T1975 2387-2388 -LRB- denotes (
T1976 2398-2404 NNP denotes Probes
T1977 2388-2397 NNP denotes Molecular
T1978 2404-2405 -RRB- denotes )
T1979 2405-2406 . denotes .
T1980 2406-2613 sentence denotes After incubation, slides were washed with PBS-TX, mounted with FluorSave (Calbiochem, La Jolla, CA), and viewed by confocal microscopy (LSM 5 Pascal or LSM 510 Meta, Carl Zeiss, Jena or Göttingen, Germany).
T1981 2407-2412 IN denotes After
T1982 2437-2443 VBN denotes washed
T1983 2413-2423 NN denotes incubation
T1984 2423-2425 , denotes ,
T1985 2425-2431 NNS denotes slides
T1986 2432-2436 VBD denotes were
T1987 2444-2448 IN denotes with
T1988 2449-2452 NN denotes PBS
T1989 2453-2455 NN denotes TX
T1990 2452-2453 HYPH denotes -
T1991 2455-2457 , denotes ,
T1992 2457-2464 VBN denotes mounted
T1993 2465-2469 IN denotes with
T1994 2470-2479 NNP denotes FluorSave
T1995 2480-2481 -LRB- denotes (
T1996 2481-2491 NNP denotes Calbiochem
T1997 2491-2493 , denotes ,
T1998 2493-2495 NNP denotes La
T1999 2496-2501 NNP denotes Jolla
T2000 2501-2503 , denotes ,
T2001 2503-2505 NNP denotes CA
T2002 2505-2506 -RRB- denotes )
T2003 2506-2508 , denotes ,
T2004 2508-2511 CC denotes and
T2005 2512-2518 VBN denotes viewed
T2006 2519-2521 IN denotes by
T2007 2522-2530 JJ denotes confocal
T2008 2531-2541 NN denotes microscopy
T2009 2542-2543 -LRB- denotes (
T2010 2549-2555 NN denotes Pascal
T2011 2543-2546 NN denotes LSM
T2012 2547-2548 CD denotes 5
T2013 2556-2558 CC denotes or
T2014 2559-2562 NN denotes LSM
T2015 2567-2571 NN denotes Meta
T2016 2563-2566 CD denotes 510
T2017 2571-2573 , denotes ,
T2018 2573-2577 NNP denotes Carl
T2019 2578-2583 NNP denotes Zeiss
T2020 2583-2585 , denotes ,
T2021 2585-2589 NNP denotes Jena
T2022 2590-2592 CC denotes or
T2023 2593-2602 NNP denotes Göttingen
T2024 2602-2604 , denotes ,
T2025 2604-2611 NNP denotes Germany
T2026 2611-2612 -RRB- denotes )
T2027 2612-2613 . denotes .
T2028 2613-2759 sentence denotes Laser scanning brightfield images were collected to document structural preservation, for morphometric analysis and for analysis of pigmentation.
T2029 2614-2619 NN denotes Laser
T2030 2620-2628 NN denotes scanning
T2031 2641-2647 NNS denotes images
T2032 2629-2640 NN denotes brightfield
T2033 2653-2662 VBN denotes collected
T2034 2648-2652 VBD denotes were
T2035 2663-2665 TO denotes to
T2036 2666-2674 VB denotes document
T2037 2675-2685 JJ denotes structural
T2038 2686-2698 NN denotes preservation
T2039 2698-2700 , denotes ,
T2040 2700-2703 IN denotes for
T2041 2704-2716 JJ denotes morphometric
T2042 2717-2725 NN denotes analysis
T2043 2726-2729 CC denotes and
T2044 2730-2733 IN denotes for
T2045 2734-2742 NN denotes analysis
T2046 2743-2745 IN denotes of
T2047 2746-2758 NN denotes pigmentation
T2048 2758-2759 . denotes .
T2210 2761-2773 JJ denotes Quantitative
T2211 2777-2780 NN denotes PCR
T2212 2774-2776 NN denotes RT
T2213 2776-2777 HYPH denotes -
T2214 2780-2857 sentence denotes Animals were deeply anesthetized with sodium pentobarbital (100 mg/kg i.p.).
T2215 2781-2788 NNS denotes Animals
T2216 2801-2813 VBN denotes anesthetized
T2217 2789-2793 VBD denotes were
T2218 2794-2800 RB denotes deeply
T2219 2814-2818 IN denotes with
T2220 2819-2825 NN denotes sodium
T2221 2826-2839 NN denotes pentobarbital
T2222 2840-2841 -LRB- denotes (
T2223 2845-2847 NN denotes mg
T2224 2841-2844 CD denotes 100
T2225 2847-2848 SYM denotes /
T2226 2848-2850 NN denotes kg
T2227 2851-2855 RB denotes i.p.
T2228 2855-2856 -RRB- denotes )
T2229 2856-2857 . denotes .
T2230 2857-2917 sentence denotes Kidneys and brain were removed and pulverized in liquid N2.
T2231 2858-2865 NNS denotes Kidneys
T2232 2881-2888 VBN denotes removed
T2233 2866-2869 CC denotes and
T2234 2870-2875 NN denotes brain
T2235 2876-2880 VBD denotes were
T2236 2889-2892 CC denotes and
T2237 2893-2903 VBN denotes pulverized
T2238 2904-2906 IN denotes in
T2239 2907-2913 NN denotes liquid
T2240 2914-2916 NN denotes N2
T2241 2916-2917 . denotes .
T2242 2917-3145 sentence denotes Temporal bones were removed and two preparations of inner ear tissues were obtained by microdissection: (1) stria vascularis without spiral ligament, and (2) spiral ganglia including the surrounding bone and the organ of Corti.
T2243 2918-2926 JJ denotes Temporal
T2244 2927-2932 NNS denotes bones
T2245 2938-2945 VBN denotes removed
T2246 2933-2937 VBD denotes were
T2247 2946-2949 CC denotes and
T2248 2950-2953 CD denotes two
T2249 2954-2966 NNS denotes preparations
T2250 2993-3001 VBN denotes obtained
T2251 2967-2969 IN denotes of
T2252 2970-2975 JJ denotes inner
T2253 2980-2987 NNS denotes tissues
T2254 2976-2979 NN denotes ear
T2255 2988-2992 VBD denotes were
T2256 3002-3004 IN denotes by
T2257 3005-3020 NN denotes microdissection
T2258 3020-3022 : denotes :
T2259 3022-3023 -LRB- denotes (
T2260 3023-3024 LS denotes 1
T2261 3032-3042 NN denotes vascularis
T2262 3024-3025 -RRB- denotes )
T2263 3026-3031 NN denotes stria
T2264 3043-3050 IN denotes without
T2265 3051-3057 JJ denotes spiral
T2266 3058-3066 NN denotes ligament
T2267 3066-3068 , denotes ,
T2268 3068-3071 CC denotes and
T2269 3072-3073 -LRB- denotes (
T2270 3073-3074 LS denotes 2
T2271 3083-3090 NNS denotes ganglia
T2272 3074-3075 -RRB- denotes )
T2273 3076-3082 JJ denotes spiral
T2274 3091-3100 VBG denotes including
T2275 3101-3104 DT denotes the
T2276 3117-3121 NN denotes bone
T2277 3105-3116 VBG denotes surrounding
T2278 3122-3125 CC denotes and
T2279 3126-3129 DT denotes the
T2280 3130-3135 NN denotes organ
T2281 3136-3138 IN denotes of
T2282 3139-3144 NNP denotes Corti
T2283 3144-3145 . denotes .
T2284 3145-3290 sentence denotes The dissection medium was changed twice during the microdissection and isolated tissues were washed three times to minimize cross-contamination.
T2285 3146-3149 DT denotes The
T2286 3161-3167 NN denotes medium
T2287 3150-3160 NN denotes dissection
T2288 3172-3179 VBN denotes changed
T2289 3168-3171 VBD denotes was
T2290 3180-3185 RB denotes twice
T2291 3186-3192 IN denotes during
T2292 3193-3196 DT denotes the
T2293 3197-3212 NN denotes microdissection
T2294 3213-3216 CC denotes and
T2295 3217-3225 VBN denotes isolated
T2296 3226-3233 NNS denotes tissues
T2297 3239-3245 VBN denotes washed
T2298 3234-3238 VBD denotes were
T2299 3246-3251 CD denotes three
T2300 3252-3257 NNS denotes times
T2301 3258-3260 TO denotes to
T2302 3261-3269 VB denotes minimize
T2303 3270-3289 NN denotes cross-contamination
T2304 3289-3290 . denotes .
T2305 3290-3393 sentence denotes The Slc26a4-/-genotype was verified by the observation of large otoconia in the utricular macula [14].
T2306 3291-3294 DT denotes The
T2307 3305-3313 NN denotes genotype
T2308 3295-3302 NN denotes Slc26a4
T2309 3302-3303 SYM denotes -
T2310 3303-3304 HYPH denotes /
T2311 3304-3305 SYM denotes -
T2312 3318-3326 VBN denotes verified
T2313 3314-3317 VBD denotes was
T2314 3327-3329 IN denotes by
T2315 3330-3333 DT denotes the
T2316 3334-3345 NN denotes observation
T2317 3346-3348 IN denotes of
T2318 3349-3354 JJ denotes large
T2319 3355-3363 NN denotes otoconia
T2320 3364-3366 IN denotes in
T2321 3367-3370 DT denotes the
T2322 3381-3387 NN denotes macula
T2323 3371-3380 JJ denotes utricular
T2324 3388-3389 -LRB- denotes [
T2325 3389-3391 CD denotes 14
T2326 3391-3392 -RRB- denotes ]
T2327 3392-3393 . denotes .
T2328 3393-3520 sentence denotes Total RNA was isolated and residual DNA contamination was removed by enzymatic digestion (RNeasy micro, Qiagen, Valencia, CA).
T2329 3394-3399 JJ denotes Total
T2330 3400-3403 NN denotes RNA
T2331 3408-3416 VBN denotes isolated
T2332 3404-3407 VBD denotes was
T2333 3417-3420 CC denotes and
T2334 3421-3429 JJ denotes residual
T2335 3434-3447 NN denotes contamination
T2336 3430-3433 NN denotes DNA
T2337 3452-3459 VBN denotes removed
T2338 3448-3451 VBD denotes was
T2339 3460-3462 IN denotes by
T2340 3463-3472 JJ denotes enzymatic
T2341 3473-3482 NN denotes digestion
T2342 3483-3484 -LRB- denotes (
T2343 3491-3496 NN denotes micro
T2344 3484-3490 NN denotes RNeasy
T2345 3496-3498 , denotes ,
T2346 3498-3504 NNP denotes Qiagen
T2347 3504-3506 , denotes ,
T2348 3506-3514 NNP denotes Valencia
T2349 3514-3516 , denotes ,
T2350 3516-3518 NNP denotes CA
T2351 3518-3519 -RRB- denotes )
T2352 3519-3520 . denotes .
T2353 3520-3657 sentence denotes Quality and quantity of 18S rRNA obtained from kidneys and brain were determined (Nano Assay, 2100 Bioanalyzer, Agilent, Palo Alto, CA).
T2354 3521-3528 NN denotes Quality
T2355 3591-3601 VBN denotes determined
T2356 3529-3532 CC denotes and
T2357 3533-3541 NN denotes quantity
T2358 3542-3544 IN denotes of
T2359 3545-3548 NN denotes 18S
T2360 3549-3553 NN denotes rRNA
T2361 3554-3562 VBN denotes obtained
T2362 3563-3567 IN denotes from
T2363 3568-3575 NNS denotes kidneys
T2364 3576-3579 CC denotes and
T2365 3580-3585 NN denotes brain
T2366 3586-3590 VBD denotes were
T2367 3602-3603 -LRB- denotes (
T2368 3608-3613 NNP denotes Assay
T2369 3603-3607 NNP denotes Nano
T2370 3613-3615 , denotes ,
T2371 3615-3619 CD denotes 2100
T2372 3620-3631 NNP denotes Bioanalyzer
T2373 3631-3633 , denotes ,
T2374 3633-3640 NNP denotes Agilent
T2375 3640-3642 , denotes ,
T2376 3642-3646 NNP denotes Palo
T2377 3647-3651 NNP denotes Alto
T2378 3651-3653 , denotes ,
T2379 3653-3655 NNP denotes CA
T2380 3655-3656 -RRB- denotes )
T2381 3656-3657 . denotes .
T2382 3657-3793 sentence denotes Further, the quality of RNA preparations obtained from stria vascularis and spiral ganglia was assessed (Pico Assay, 2100 Bioanalyzer).
T2383 3658-3665 RB denotes Further
T2384 3753-3761 VBN denotes assessed
T2385 3665-3667 , denotes ,
T2386 3667-3670 DT denotes the
T2387 3671-3678 NN denotes quality
T2388 3679-3681 IN denotes of
T2389 3682-3685 NN denotes RNA
T2390 3686-3698 NNS denotes preparations
T2391 3699-3707 VBN denotes obtained
T2392 3708-3712 IN denotes from
T2393 3713-3718 NN denotes stria
T2394 3719-3729 NN denotes vascularis
T2395 3730-3733 CC denotes and
T2396 3734-3740 JJ denotes spiral
T2397 3741-3748 NNS denotes ganglia
T2398 3749-3752 VBD denotes was
T2399 3762-3763 -LRB- denotes (
T2400 3768-3773 NNP denotes Assay
T2401 3763-3767 NNP denotes Pico
T2402 3773-3775 , denotes ,
T2403 3775-3779 CD denotes 2100
T2404 3780-3791 NNP denotes Bioanalyzer
T2405 3791-3792 -RRB- denotes )
T2406 3792-3793 . denotes .
T2407 3793-3984 sentence denotes Real time RT-PCR was performed on RNA obtained from individual animals (OneStep RT-PCR, Qiagen; Smart Cycler, Cepheid, Sunnyvale, CA) in the presence of 0.2× SYBR green I (Molecular Probes).
T2408 3794-3798 JJ denotes Real
T2409 3799-3803 NN denotes time
T2410 3807-3810 NN denotes PCR
T2411 3804-3806 NN denotes RT
T2412 3806-3807 HYPH denotes -
T2413 3815-3824 VBN denotes performed
T2414 3811-3814 VBD denotes was
T2415 3825-3827 IN denotes on
T2416 3828-3831 NN denotes RNA
T2417 3832-3840 VBN denotes obtained
T2418 3841-3845 IN denotes from
T2419 3846-3856 JJ denotes individual
T2420 3857-3864 NNS denotes animals
T2421 3865-3866 -LRB- denotes (
T2422 3896-3902 NNP denotes Cycler
T2423 3866-3873 NNP denotes OneStep
T2424 3877-3880 NN denotes PCR
T2425 3874-3876 NN denotes RT
T2426 3876-3877 HYPH denotes -
T2427 3880-3882 , denotes ,
T2428 3882-3888 NNP denotes Qiagen
T2429 3888-3889 : denotes ;
T2430 3890-3895 NNP denotes Smart
T2431 3902-3904 , denotes ,
T2432 3904-3911 NNP denotes Cepheid
T2433 3911-3913 , denotes ,
T2434 3913-3922 NNP denotes Sunnyvale
T2435 3922-3924 , denotes ,
T2436 3924-3926 NNP denotes CA
T2437 3926-3927 -RRB- denotes )
T2438 3928-3930 IN denotes in
T2439 3931-3934 DT denotes the
T2440 3935-3943 NN denotes presence
T2441 3944-3946 IN denotes of
T2442 3947-3950 CD denotes 0.2
T2443 3952-3956 NN denotes SYBR
T2444 3950-3951 SYM denotes ×
T2445 3963-3964 NN denotes I
T2446 3957-3962 JJ denotes green
T2447 3965-3966 -LRB- denotes (
T2448 3976-3982 NNP denotes Probes
T2449 3966-3975 NNP denotes Molecular
T2450 3982-3983 -RRB- denotes )
T2451 3983-3984 . denotes .
T2452 3984-4115 sentence denotes Transcripts of 18S rRNA and mRNA for the K+ channels KCNJ10, KCNQ1 and KCNQ4 were amplified using gene-specific primers (Table 1).
T2453 3985-3996 NNS denotes Transcripts
T2454 4067-4076 VBN denotes amplified
T2455 3997-3999 IN denotes of
T2456 4000-4003 NN denotes 18S
T2457 4004-4008 NN denotes rRNA
T2458 4009-4012 CC denotes and
T2459 4013-4017 NN denotes mRNA
T2460 4018-4021 IN denotes for
T2461 4022-4025 DT denotes the
T2462 4029-4037 NNS denotes channels
T2463 4026-4027 NN denotes K
T2464 4027-4028 SYM denotes +
T2465 4038-4044 NN denotes KCNJ10
T2466 4044-4046 , denotes ,
T2467 4046-4051 NN denotes KCNQ1
T2468 4052-4055 CC denotes and
T2469 4056-4061 NN denotes KCNQ4
T2470 4062-4066 VBD denotes were
T2471 4077-4082 VBG denotes using
T2472 4083-4087 NN denotes gene
T2473 4088-4096 JJ denotes specific
T2474 4087-4088 HYPH denotes -
T2475 4097-4104 NNS denotes primers
T2476 4105-4106 -LRB- denotes (
T2477 4106-4111 NN denotes Table
T2478 4112-4113 CD denotes 1
T2479 4113-4114 -RRB- denotes )
T2480 4114-4115 . denotes .
T2481 4115-4171 sentence denotes RT was performed for 30 min at 50°C and 15 min at 95°C.
T2482 4116-4118 NN denotes RT
T2483 4123-4132 VBN denotes performed
T2484 4119-4122 VBD denotes was
T2485 4133-4136 IN denotes for
T2486 4137-4139 CD denotes 30
T2487 4140-4143 NN denotes min
T2488 4144-4146 IN denotes at
T2489 4147-4149 CD denotes 50
T2490 4149-4151 NN denotes °C
T2491 4152-4155 CC denotes and
T2492 4156-4158 CD denotes 15
T2493 4159-4162 NN denotes min
T2494 4163-4165 IN denotes at
T2495 4166-4168 CD denotes 95
T2496 4168-4170 NN denotes °C
T2497 4170-4171 . denotes .
T2498 4171-4366 sentence denotes PCR consisted of 50 cycles of 1 min at 60°C, 1 min at 72°C, 7s heating to hot-measurement temperature, 13s hot-measurement at 3–5°C below product melting temperature (Table 1) and 1 min at 94°C.
T2499 4172-4175 NN denotes PCR
T2500 4176-4185 VBD denotes consisted
T2501 4186-4188 IN denotes of
T2502 4189-4191 CD denotes 50
T2503 4192-4198 NNS denotes cycles
T2504 4199-4201 IN denotes of
T2505 4202-4203 CD denotes 1
T2506 4204-4207 NN denotes min
T2507 4208-4210 IN denotes at
T2508 4211-4213 CD denotes 60
T2509 4213-4215 NN denotes °C
T2510 4215-4217 , denotes ,
T2511 4217-4218 CD denotes 1
T2512 4219-4222 NN denotes min
T2513 4223-4225 IN denotes at
T2514 4226-4228 CD denotes 72
T2515 4228-4230 NN denotes °C
T2516 4230-4232 , denotes ,
T2517 4232-4234 NN denotes 7s
T2518 4235-4242 VBG denotes heating
T2519 4243-4245 IN denotes to
T2520 4246-4249 JJ denotes hot
T2521 4250-4261 NN denotes measurement
T2522 4249-4250 HYPH denotes -
T2523 4262-4273 NN denotes temperature
T2524 4273-4275 , denotes ,
T2525 4275-4278 NNS denotes 13s
T2526 4283-4294 NN denotes measurement
T2527 4279-4282 NN denotes hot
T2528 4282-4283 HYPH denotes -
T2529 4295-4297 IN denotes at
T2530 4298-4299 CD denotes 3
T2531 4300-4301 CD denotes 5
T2532 4299-4300 SYM denotes –
T2533 4301-4303 NN denotes °C
T2534 4304-4309 IN denotes below
T2535 4310-4317 NN denotes product
T2536 4326-4337 NN denotes temperature
T2537 4318-4325 NN denotes melting
T2538 4338-4339 -LRB- denotes (
T2539 4339-4344 NN denotes Table
T2540 4345-4346 CD denotes 1
T2541 4346-4347 -RRB- denotes )
T2542 4348-4351 CC denotes and
T2543 4352-4353 CD denotes 1
T2544 4354-4357 NN denotes min
T2545 4358-4360 IN denotes at
T2546 4361-4363 CD denotes 94
T2547 4363-4365 NN denotes °C
T2548 4365-4366 . denotes .
T2549 4366-4498 sentence denotes Hot-measurements were performed to eliminate the detection of primer-dimers that had melting temperatures between 72 and 75°C [15].
T2550 4367-4370 JJ denotes Hot
T2551 4371-4383 NNS denotes measurements
T2552 4370-4371 HYPH denotes -
T2553 4389-4398 VBN denotes performed
T2554 4384-4388 VBD denotes were
T2555 4399-4401 TO denotes to
T2556 4402-4411 VB denotes eliminate
T2557 4412-4415 DT denotes the
T2558 4416-4425 NN denotes detection
T2559 4426-4428 IN denotes of
T2560 4429-4435 NN denotes primer
T2561 4436-4442 NNS denotes dimers
T2562 4435-4436 HYPH denotes -
T2563 4443-4447 WDT denotes that
T2564 4448-4451 VBD denotes had
T2565 4452-4459 NN denotes melting
T2566 4460-4472 NNS denotes temperatures
T2567 4473-4480 IN denotes between
T2568 4481-4483 CD denotes 72
T2569 4490-4492 NN denotes °C
T2570 4484-4487 CC denotes and
T2571 4488-4490 CD denotes 75
T2572 4493-4494 -LRB- denotes [
T2573 4494-4496 CD denotes 15
T2574 4496-4497 -RRB- denotes ]
T2575 4497-4498 . denotes .
T2576 4498-4539 sentence denotes PCR was followed by a melt (60 to 95°C).
T2577 4499-4502 NN denotes PCR
T2578 4507-4515 VBN denotes followed
T2579 4503-4506 VBD denotes was
T2580 4516-4518 IN denotes by
T2581 4519-4520 DT denotes a
T2582 4521-4525 NN denotes melt
T2583 4526-4527 -LRB- denotes (
T2584 4535-4537 NN denotes °C
T2585 4527-4529 CD denotes 60
T2586 4533-4535 CD denotes 95
T2592 4544-4554 NN denotes generation
T2593 4609-4616 VBN denotes checked
T2594 4555-4557 IN denotes of
T2595 4558-4559 DT denotes a
T2596 4567-4574 NN denotes product
T2597 4560-4566 JJ denotes single
T2598 4575-4577 IN denotes of
T2599 4578-4589 JJ denotes appropriate
T2600 4590-4594 NN denotes size
T2601 4595-4598 VBD denotes was
T2602 4599-4608 RB denotes routinely
T2603 4617-4619 IN denotes by
T2604 4620-4623 DT denotes the
T2605 4624-4632 NN denotes presence
T2606 4633-4635 IN denotes of
T2607 4636-4637 DT denotes a
T2608 4650-4654 NN denotes peak
T2609 4638-4644 JJ denotes single
T2610 4645-4649 NN denotes melt
T2611 4655-4658 CC denotes and
T2612 4659-4661 IN denotes by
T2613 4662-4669 NN denotes agarose
T2614 4670-4673 NN denotes gel
T2615 4674-4689 NN denotes electrophoresis
T2616 4673-4674 HYPH denotes -
T2617 4689-4690 . denotes .
T2618 4690-4736 sentence denotes Product identity was confirmed by sequencing.
T2619 4691-4698 NN denotes Product
T2620 4699-4707 NN denotes identity
T2621 4712-4721 VBN denotes confirmed
T2622 4708-4711 VBD denotes was
T2623 4722-4724 IN denotes by
T2624 4725-4735 NN denotes sequencing
T2625 4735-4736 . denotes .
T2627 4737-4762 NN denotes Table 1 Primers Template
T7759 4746-4753 NNS denotes Primers
T1678 1055-1063 JJ denotes Confocal
T2587 4530-4532 IN denotes to
T2588 4537-4538 -RRB- denotes )
T2589 4538-4539 . denotes .
T2590 4539-4690 sentence denotes The generation of a single product of appropriate size was routinely checked by the presence of a single melt peak and by agarose gel-electrophoresis.
T2591 4540-4543 DT denotes The
R753 T1324 T1320 nmod K,concentrations
R754 T1325 T1324 punct +,K
R755 T1326 T1317 auxpass were,measured
R756 T1327 T1317 prep in,measured
R757 T1328 T1329 amod young,mice
R758 T1329 T1327 pobj mice,in
R759 T1330 T1329 amod adult,mice
R760 T1331 T1332 punct (,month
R761 T1332 T1329 parataxis month,mice
R762 T1333 T1334 quantmod 1,4
R763 T1334 T1332 nummod 4,month
R764 T1335 T1334 punct –,4
R765 T1336 T1332 prep of,month
R766 T1337 T1336 pobj age,of
R767 T1338 T1332 punct ),month
R768 T1339 T1340 dep that,have
R769 T1340 T1329 relcl have,mice
R770 T1341 T1340 preconj either,have
R771 T1342 T1343 punct (,Slc26a4
R772 T1343 T1340 parataxis Slc26a4,have
R773 T1344 T1343 punct +,Slc26a4
R774 T1345 T1343 punct /,Slc26a4
R775 T1346 T1343 punct +,Slc26a4
R776 T1347 T1343 punct ),Slc26a4
R777 T1348 T1340 cc or,have
R778 T1349 T1340 conj lack,have
R779 T1350 T1351 punct (,Slc26a4
R780 T1351 T1349 parataxis Slc26a4,lack
R781 T1352 T1351 punct -,Slc26a4
R782 T1353 T1351 punct /,Slc26a4
R783 T1354 T1351 punct -,Slc26a4
R784 T1355 T1351 punct ),Slc26a4
R785 T1356 T1357 det a,gene
R786 T1357 T1349 dobj gene,lack
R787 T1358 T1357 amod functional,gene
R788 T1359 T1357 prep for,gene
R789 T1360 T1359 pobj pendrin,for
R790 T1361 T1362 punct [,14
R791 T1362 T1349 parataxis 14,lack
R792 T1363 T1362 punct ],14
R793 T1364 T1317 punct .,measured
R794 T1366 T1367 det The,strain
R795 T1367 T1369 nsubjpass strain,chosen
R796 T1368 T1367 compound mouse,strain
R797 T1370 T1371 compound 129Sv,Ev
R798 T1371 T1367 appos Ev,strain
R799 T1372 T1371 punct /,Ev
R800 T1373 T1374 punct (,Taconic
R801 T1374 T1367 parataxis Taconic,strain
R802 T1375 T1374 punct ", ",Taconic
R803 T1376 T1374 npadvmod Germantown,Taconic
R804 T1377 T1374 punct ", ",Taconic
R805 T1378 T1374 npadvmod NY,Taconic
R806 T1379 T1374 punct ),Taconic
R807 T1380 T1369 auxpass was,chosen
R808 T1381 T1369 prep as,chosen
R809 T1382 T1383 det the,source
R810 T1383 T1381 pobj source,as
R811 T1384 T1383 prep of,source
R812 T1385 T1386 nmod Slc26a4,controls
R813 T1386 T1384 pobj controls,of
R814 T1387 T1385 punct +,Slc26a4
R815 T1388 T1385 punct /,Slc26a4
R816 T1389 T1385 punct +,Slc26a4
R817 T1390 T1369 punct ", ",chosen
R818 T1391 T1392 mark since,propagated
R819 T1392 T1369 advcl propagated,chosen
R820 T1393 T1394 nmod Slc26a4,mice
R821 T1394 T1392 nsubjpass mice,propagated
R822 T1395 T1393 punct -,Slc26a4
R823 T1396 T1393 punct /,Slc26a4
R824 T1397 T1393 punct -,Slc26a4
R825 T1398 T1392 auxpass were,propagated
R826 T1399 T1392 prep in,propagated
R827 T1400 T1401 nmod the,strain
R828 T1401 T1399 pobj strain,in
R829 T1402 T1401 det this,strain
R830 T1403 T1392 cc and,propagated
R831 T1404 T1392 conj generated,propagated
R832 T1405 T1404 advcl using,generated
R833 T1406 T1407 det a,line
R834 T1407 T1405 dobj line,using
R835 T1408 T1409 compound stem,cell
R836 T1409 T1407 compound cell,line
R837 T1410 T1407 acl derived,line
R838 T1411 T1410 prep from,derived
R839 T1412 T1413 compound 129Sv,Ev
R840 T1413 T1411 pobj Ev,from
R841 T1414 T1413 punct /,Ev
R842 T1415 T1369 punct .,chosen
R843 T1417 T1418 nsubj Slc26a4,were
R844 T1419 T1417 punct +,Slc26a4
R845 T1420 T1417 punct /,Slc26a4
R846 T1421 T1417 punct +,Slc26a4
R847 T1422 T1417 cc and,Slc26a4
R848 T1423 T1417 conj Slc26a4,Slc26a4
R849 T1424 T1423 punct -,Slc26a4
R850 T1425 T1423 punct /,Slc26a4
R851 T1426 T1423 punct -,Slc26a4
R852 T1427 T1418 attr agouti,were
R853 T1428 T1418 punct .,were
R854 T1430 T1431 nsubj They,differ
R855 T1432 T1431 aux did,differ
R856 T1433 T1431 neg not,differ
R857 T1434 T1431 prep in,differ
R858 T1435 T1436 compound coat,color
R859 T1436 T1434 pobj color,in
R860 T1437 T1431 punct .,differ
R861 T1439 T1440 nsubjpass Differences,verified
R862 T1441 T1439 prep in,Differences
R863 T1442 T1441 pobj pigmentation,in
R864 T1443 T1440 auxpass were,verified
R865 T1444 T1440 advcl using,verified
R866 T1445 T1446 amod coisogenic,Slc26a4
R867 T1446 T1444 dobj Slc26a4,using
R868 T1447 T1448 npadvmod age,matched
R869 T1448 T1446 amod matched,Slc26a4
R870 T1449 T1448 punct -,matched
R871 T1450 T1446 punct +,Slc26a4
R872 T1451 T1446 punct /,Slc26a4
R873 T1452 T1446 punct +,Slc26a4
R874 T1453 T1446 cc and,Slc26a4
R875 T1454 T1446 conj Slc26a4,Slc26a4
R876 T1455 T1454 punct -,Slc26a4
R877 T1456 T1454 punct /,Slc26a4
R878 T1457 T1454 punct -,Slc26a4
R879 T1458 T1459 dep that,bred
R880 T1459 T1446 relcl bred,Slc26a4
R881 T1460 T1459 auxpass were,bred
R882 T1461 T1459 prep in,bred
R883 T1462 T1461 pobj parallel,in
R884 T1463 T1440 punct .,verified
R885 T1465 T1466 nsubjpass Expression,studied
R886 T1467 T1465 prep of,Expression
R887 T1468 T1469 amod key,proteins
R888 T1469 T1467 pobj proteins,of
R889 T1470 T1469 acl involved,proteins
R890 T1471 T1470 prep in,involved
R891 T1472 T1473 det the,generation
R892 T1473 T1471 pobj generation,in
R893 T1474 T1473 prep of,generation
R894 T1475 T1476 det the,potential
R895 T1476 T1474 pobj potential,of
R896 T1477 T1476 amod endocochlear,potential
R897 T1478 T1473 cc and,generation
R898 T1479 T1480 det the,transport
R899 T1480 T1473 conj transport,generation
R900 T1481 T1480 prep of,transport
R901 T1482 T1481 pobj K,of
R902 T1483 T1482 punct +,K
R903 T1484 T1466 auxpass was,studied
R904 T1485 T1466 advcl using,studied
R905 T1486 T1487 amod confocal,immunocytochemistry
R906 T1487 T1485 dobj immunocytochemistry,using
R907 T1488 T1487 cc and,immunocytochemistry
R908 T1489 T1490 amod quantitative,PCR
R909 T1490 T1487 conj PCR,immunocytochemistry
R910 T1491 T1490 compound RT,PCR
R911 T1492 T1490 punct -,PCR
R912 T1493 T1466 punct .,studied
R913 T1495 T1496 nmod K,secretion
R914 T1496 T1498 nsubjpass secretion,measured
R915 T1497 T1495 punct +,K
R916 T1499 T1496 cc and,secretion
R917 T1500 T1501 det the,generation
R918 T1501 T1496 conj generation,secretion
R919 T1502 T1501 prep of,generation
R920 T1503 T1504 det the,potential
R921 T1504 T1502 pobj potential,of
R922 T1505 T1504 amod endocochlear,potential
R923 T1506 T1498 auxpass were,measured
R924 T1507 T1498 advcl using,measured
R925 T1508 T1509 amod electrophysiological,techniques
R926 T1509 T1507 dobj techniques,using
R927 T1510 T1498 punct .,measured
R928 T1512 T1513 det All,experiments
R929 T1513 T1514 nsubjpass experiments,approved
R930 T1515 T1514 auxpass were,approved
R931 T1516 T1514 agent by,approved
R932 T1517 T1518 det the,Committee
R933 T1518 T1516 pobj Committee,by
R934 T1519 T1520 nmod Institutional,Animal
R935 T1520 T1518 nmod Animal,Committee
R936 T1521 T1520 appos Care,Animal
R937 T1522 T1521 cc and,Care
R938 T1523 T1521 conj Use,Care
R939 T1524 T1518 prep of,Committee
R940 T1525 T1526 compound Kansas,University
R941 T1526 T1524 pobj University,of
R942 T1527 T1526 compound State,University
R943 T1528 T1514 punct .,approved
R944 T1678 T1679 amod Confocal,immunocytochemistry
R945 T1681 T1682 nsubjpass Animals,anesthetized
R946 T1683 T1682 auxpass were,anesthetized
R947 T1684 T1682 advmod deeply,anesthetized
R948 T1685 T1682 prep with,anesthetized
R949 T1686 T1687 compound sodium,pentobarbital
R950 T1687 T1685 pobj pentobarbital,with
R951 T1688 T1689 punct (,mg
R952 T1689 T1687 parataxis mg,pentobarbital
R953 T1690 T1689 nummod 100,mg
R954 T1691 T1692 punct /,kg
R955 T1692 T1689 prep kg,mg
R956 T1693 T1689 advmod i.p.,mg
R957 T1694 T1689 punct ),mg
R958 T1695 T1682 cc and,anesthetized
R959 T1696 T1697 advmod transcardially,perfused
R960 T1697 T1682 conj perfused,anesthetized
R961 T1698 T1697 prep with,perfused
R962 T1699 T1700 det a,solution
R963 T1700 T1698 pobj solution,with
R964 T1701 T1702 npadvmod Cl,free
R965 T1702 T1700 amod free,solution
R966 T1703 T1701 punct -,Cl
R967 T1704 T1702 punct -,free
R968 T1705 T1706 npadvmod phosphate,buffered
R969 T1706 T1700 amod buffered,solution
R970 T1707 T1706 punct -,buffered
R971 T1708 T1709 nmod Na,gluconate
R972 T1709 T1700 compound gluconate,solution
R973 T1710 T1708 punct -,Na
R974 T1711 T1700 acl containing,solution
R975 T1712 T1713 nummod 4,%
R976 T1713 T1714 compound %,paraformaldehyde
R977 T1714 T1711 dobj paraformaldehyde,containing
R978 T1715 T1682 punct .,anesthetized
R979 T1717 T1718 amod Temporal,bones
R980 T1718 T1719 nsubjpass bones,removed
R981 T1720 T1719 auxpass were,removed
R982 T1721 T1719 cc and,removed
R983 T1722 T1723 nsubj cochleae,fixed
R984 T1723 T1719 conj fixed,removed
R985 T1724 T1723 prep by,fixed
R986 T1725 T1726 amod perilymphatic,perfusion
R987 T1726 T1724 pobj perfusion,by
R988 T1727 T1723 punct ", ",fixed
R989 T1728 T1723 conj decalcified,fixed
R990 T1729 T1728 prep in,decalcified
R991 T1730 T1729 pobj EDTA,in
R992 T1731 T1728 punct ", ",decalcified
R993 T1732 T1728 conj processed,decalcified
R994 T1733 T1732 prep through,processed
R995 T1734 T1735 det a,gradient
R996 T1735 T1733 pobj gradient,through
R997 T1736 T1735 compound sucrose,gradient
R998 T1737 T1732 cc and,processed
R999 T1738 T1732 conj infiltrated,processed
R1000 T1739 T1738 prep with,infiltrated
R1001 T1740 T1741 compound polyethylene,glycol
R1002 T1741 T1739 pobj glycol,with
R1003 T1742 T1723 punct .,fixed
R1004 T1744 T1745 amod Mid-modiolar,cryosections
R1005 T1745 T1746 nsubjpass cryosections,blocked
R1006 T1747 T1748 punct (,CM3050S
R1007 T1748 T1745 parataxis CM3050S,cryosections
R1008 T1749 T1750 nummod 12,μm
R1009 T1750 T1748 nmod μm,CM3050S
R1010 T1751 T1748 punct ", ",CM3050S
R1011 T1752 T1748 punct ", ",CM3050S
R1012 T1753 T1748 npadvmod Leica,CM3050S
R1013 T1754 T1748 punct ", ",CM3050S
R1014 T1755 T1748 npadvmod Nussloch,CM3050S
R1015 T1756 T1748 punct ", ",CM3050S
R1016 T1757 T1748 npadvmod Germany,CM3050S
R1017 T1758 T1748 punct ),CM3050S
R1018 T1759 T1746 auxpass were,blocked
R1019 T1760 T1746 prep in,blocked
R1020 T1761 T1760 pobj PBS,in
R1021 T1762 T1746 prep with,blocked
R1022 T1763 T1764 nummod 0.2,%
R1023 T1764 T1765 compound %,X
R1024 T1765 T1762 pobj X,with
R1025 T1766 T1765 compound Triton,X
R1026 T1767 T1765 punct -,X
R1027 T1768 T1769 punct (,TX
R1028 T1769 T1765 parataxis TX,X
R1029 T1770 T1769 compound PBS,TX
R1030 T1771 T1769 punct -,TX
R1031 T1772 T1769 punct ),TX
R1032 T1773 T1765 cc and,X
R1033 T1774 T1775 nummod 10,%
R1034 T1775 T1776 nmod %,albumin
R1035 T1776 T1765 conj albumin,X
R1036 T1777 T1776 amod bovine,albumin
R1037 T1778 T1776 compound serum,albumin
R1038 T1779 T1746 punct .,blocked
R1039 T1781 T1782 nsubjpass Slides,incubated
R1040 T1783 T1782 auxpass were,incubated
R1041 T1784 T1782 advmod overnight,incubated
R1042 T1785 T1782 prep at,incubated
R1043 T1786 T1787 nummod 4,°C
R1044 T1787 T1785 pobj °C,at
R1045 T1788 T1782 prep with,incubated
R1046 T1789 T1790 amod primary,antibodies
R1047 T1790 T1788 pobj antibodies,with
R1048 T1791 T1782 prep in,incubated
R1049 T1792 T1793 compound PBS,TX
R1050 T1793 T1791 pobj TX,in
R1051 T1794 T1793 punct -,TX
R1052 T1795 T1782 prep with,incubated
R1053 T1796 T1797 quantmod 1,3
R1054 T1797 T1799 nummod 3,%
R1055 T1798 T1797 punct –,3
R1056 T1799 T1800 compound %,BSA
R1057 T1800 T1795 pobj BSA,with
R1058 T1801 T1802 punct [,h766
R1059 T1802 T1782 parataxis h766,incubated
R1060 T1803 T1804 compound rabbit,anti-pendrin
R1061 T1804 T1802 dep anti-pendrin,h766
R1062 T1805 T1802 punct ", ",h766
R1063 T1806 T1802 dep 1,h766
R1064 T1807 T1808 punct :,500
R1065 T1808 T1806 prep 500,1
R1066 T1809 T1802 punct (,h766
R1067 T1810 T1811 punct –,780
R1068 T1811 T1802 prep 780,h766
R1069 T1812 T1802 punct ),h766
R1070 T1813 T1802 punct ;,h766
R1071 T1814 T1815 compound rat,anti-ZO
R1072 T1815 T1816 dep anti-ZO,Chemicon
R1073 T1816 T1802 conj Chemicon,h766
R1074 T1817 T1815 punct -,anti-ZO
R1075 T1818 T1815 nummod 1,anti-ZO
R1076 T1819 T1816 punct ", ",Chemicon
R1077 T1820 T1816 dep 1,Chemicon
R1078 T1821 T1822 punct :,100
R1079 T1822 T1820 prep 100,1
R1080 T1823 T1816 punct (,Chemicon
R1081 T1824 T1816 punct ", ",Chemicon
R1082 T1825 T1816 npadvmod Temecula,Chemicon
R1083 T1826 T1816 punct ", ",Chemicon
R1084 T1827 T1816 npadvmod CA,Chemicon
R1085 T1828 T1816 punct ),Chemicon
R1086 T1829 T1816 punct ;,Chemicon
R1087 T1830 T1831 compound goat,anti-KCNQ1
R1088 T1831 T1832 dep anti-KCNQ1,C20
R1089 T1832 T1816 conj C20,Chemicon
R1090 T1833 T1832 punct ", ",C20
R1091 T1834 T1832 dep 1,C20
R1092 T1835 T1836 punct :,200
R1093 T1836 T1834 prep 200,1
R1094 T1837 T1832 punct (,C20
R1095 T1838 T1832 punct ", ",C20
R1096 T1839 T1840 compound Santa,Cruz
R1097 T1840 T1841 compound Cruz,Biotechnology
R1098 T1841 T1832 npadvmod Biotechnology,C20
R1099 T1842 T1832 punct ", ",C20
R1100 T1843 T1844 compound Santa,Cruz
R1101 T1844 T1832 npadvmod Cruz,C20
R1102 T1845 T1832 punct ", ",C20
R1103 T1846 T1832 npadvmod CA,C20
R1104 T1847 T1832 punct ),C20
R1105 T1848 T1832 punct ", ",C20
R1106 T1849 T1850 compound rabbit,anti-KCNE1
R1107 T1850 T1851 dep anti-KCNE1,Alomone
R1108 T1851 T1832 conj Alomone,C20
R1109 T1852 T1851 punct ", ",Alomone
R1110 T1853 T1851 dep 1,Alomone
R1111 T1854 T1855 punct :,200
R1112 T1855 T1853 prep 200,1
R1113 T1856 T1851 punct (,Alomone
R1114 T1857 T1851 punct ", ",Alomone
R1115 T1858 T1851 npadvmod Jerusalem,Alomone
R1116 T1859 T1851 punct ", ",Alomone
R1117 T1860 T1851 npadvmod Israel,Alomone
R1118 T1861 T1851 punct ),Alomone
R1119 T1862 T1851 punct ;,Alomone
R1120 T1863 T1864 compound rabbit,anti-KCNJ10
R1121 T1864 T1865 dep anti-KCNJ10,Alomone
R1122 T1865 T1851 conj Alomone,Alomone
R1123 T1866 T1865 punct ", ",Alomone
R1124 T1867 T1865 dep 1,Alomone
R1125 T1868 T1869 punct :,300
R1126 T1869 T1867 prep 300,1
R1127 T1870 T1865 punct (,Alomone
R1128 T1871 T1865 punct ),Alomone
R1129 T1872 T1865 punct ;,Alomone
R1130 T1873 T1874 compound rabbit,anti-SLC12A2
R1131 T1874 T1875 dep anti-SLC12A2,Chemicon
R1132 T1875 T1865 conj Chemicon,Alomone
R1133 T1876 T1875 punct ", ",Chemicon
R1134 T1877 T1875 dep 1,Chemicon
R1135 T1878 T1879 punct :,100
R1136 T1879 T1877 prep 100,1
R1137 T1880 T1875 punct (,Chemicon
R1138 T1881 T1875 punct ),Chemicon
R1139 T1882 T1875 punct ;,Chemicon
R1140 T1883 T1875 cc and,Chemicon
R1141 T1884 T1885 compound rabbit,anti-connexin
R1142 T1885 T1886 dep anti-connexin,Zymed
R1143 T1886 T1875 conj Zymed,Chemicon
R1144 T1887 T1885 nummod 26,anti-connexin
R1145 T1888 T1886 punct ", ",Zymed
R1146 T1889 T1886 dep 1,Zymed
R1147 T1890 T1891 punct :,100
R1148 T1891 T1889 prep 100,1
R1149 T1892 T1886 punct (,Zymed
R1150 T1893 T1886 punct ", ",Zymed
R1151 T1894 T1895 compound San,Francisco
R1152 T1895 T1886 npadvmod Francisco,Zymed
R1153 T1896 T1886 punct ", ",Zymed
R1154 T1897 T1886 npadvmod CA,Zymed
R1155 T1898 T1886 punct ),Zymed
R1156 T1899 T1886 punct ],Zymed
R1157 T1900 T1782 punct .,incubated
R1158 T1902 T1903 nsubjpass Slides,washed
R1159 T1904 T1903 auxpass were,washed
R1160 T1905 T1903 prep in,washed
R1161 T1906 T1907 compound PBS,TX
R1162 T1907 T1905 pobj TX,in
R1163 T1908 T1907 punct -,TX
R1164 T1909 T1903 cc and,washed
R1165 T1910 T1903 conj incubated,washed
R1166 T1911 T1910 prep for,incubated
R1167 T1912 T1913 nummod 1,h
R1168 T1913 T1911 pobj h,for
R1169 T1914 T1910 prep at,incubated
R1170 T1915 T1916 nummod 25,°C
R1171 T1916 T1914 pobj °C,at
R1172 T1917 T1910 prep with,incubated
R1173 T1918 T1919 amod appropriate,antibodies
R1174 T1919 T1917 pobj antibodies,with
R1175 T1920 T1919 amod secondary,antibodies
R1176 T1921 T1919 prep at,antibodies
R1177 T1922 T1923 det a,dilution
R1178 T1923 T1921 pobj dilution,at
R1179 T1924 T1923 nummod 1,dilution
R1180 T1925 T1926 punct :,1000
R1181 T1926 T1924 prep 1000,1
R1182 T1927 T1923 prep in,dilution
R1183 T1928 T1929 compound PBS,TX
R1184 T1929 T1927 pobj TX,in
R1185 T1930 T1929 punct -,TX
R1186 T1931 T1923 prep with,dilution
R1187 T1932 T1933 quantmod 1,3
R1188 T1933 T1935 nummod 3,%
R1189 T1934 T1933 punct –,3
R1190 T1935 T1936 compound %,BSA
R1191 T1936 T1931 pobj BSA,with
R1192 T1937 T1938 punct [,Probes
R1193 T1938 T1910 parataxis Probes,incubated
R1194 T1939 T1940 nmod donkey,Alexa
R1195 T1940 T1938 dep Alexa,Probes
R1196 T1941 T1939 amod anti-rabbit,donkey
R1197 T1942 T1940 nummod 488,Alexa
R1198 T1943 T1940 punct ", ",Alexa
R1199 T1944 T1945 nmod chicken,Alexa
R1200 T1945 T1940 conj Alexa,Alexa
R1201 T1946 T1944 amod anti-rat,chicken
R1202 T1947 T1945 nummod 594,Alexa
R1203 T1948 T1945 punct ", ",Alexa
R1204 T1949 T1945 cc and,Alexa
R1205 T1950 T1951 nmod chicken,Alexa
R1206 T1951 T1945 conj Alexa,Alexa
R1207 T1952 T1950 amod anti-goat,chicken
R1208 T1953 T1951 nummod 594,Alexa
R1209 T1954 T1938 punct (,Probes
R1210 T1955 T1938 compound Molecular,Probes
R1211 T1956 T1938 punct ", ",Probes
R1212 T1957 T1938 npadvmod Eugene,Probes
R1213 T1958 T1938 punct ", ",Probes
R1214 T1959 T1938 npadvmod OR,Probes
R1215 T1960 T1938 punct ),Probes
R1216 T1961 T1938 punct ],Probes
R1217 T1962 T1903 punct .,washed
R1218 T1964 T1965 compound Actin,filaments
R1219 T1965 T1966 nsubjpass filaments,visualized
R1220 T1967 T1966 auxpass were,visualized
R1221 T1968 T1966 prep by,visualized
R1222 T1969 T1968 pcomp staining,by
R1223 T1970 T1969 prep with,staining
R1224 T1971 T1972 npadvmod Alexa,conjugated
R1225 T1972 T1974 amod conjugated,phalloidin
R1226 T1973 T1971 nummod 488,Alexa
R1227 T1974 T1970 pobj phalloidin,with
R1228 T1975 T1976 punct (,Probes
R1229 T1976 T1974 parataxis Probes,phalloidin
R1230 T1977 T1976 compound Molecular,Probes
R1231 T1978 T1976 punct ),Probes
R1232 T1979 T1966 punct .,visualized
R1233 T1981 T1982 prep After,washed
R1234 T1983 T1981 pobj incubation,After
R1235 T1984 T1982 punct ", ",washed
R1236 T1985 T1982 nsubjpass slides,washed
R1237 T1986 T1982 auxpass were,washed
R1238 T1987 T1982 prep with,washed
R1239 T1988 T1989 compound PBS,TX
R1240 T1989 T1987 pobj TX,with
R1241 T1990 T1989 punct -,TX
R1242 T1991 T1982 punct ", ",washed
R1243 T1992 T1982 conj mounted,washed
R1244 T1993 T1992 prep with,mounted
R1245 T1994 T1993 pobj FluorSave,with
R1246 T1995 T1996 punct (,Calbiochem
R1247 T1996 T1994 parataxis Calbiochem,FluorSave
R1248 T1997 T1996 punct ", ",Calbiochem
R1249 T1998 T1999 compound La,Jolla
R1250 T1999 T1996 npadvmod Jolla,Calbiochem
R1251 T2000 T1996 punct ", ",Calbiochem
R1252 T2001 T1996 npadvmod CA,Calbiochem
R1253 T2002 T1996 punct ),Calbiochem
R1254 T2003 T1992 punct ", ",mounted
R1255 T2004 T1992 cc and,mounted
R1256 T2005 T1992 conj viewed,mounted
R1257 T2006 T2005 prep by,viewed
R1258 T2007 T2008 amod confocal,microscopy
R1259 T2008 T2006 pobj microscopy,by
R1260 T2009 T2010 punct (,Pascal
R1261 T2010 T2008 parataxis Pascal,microscopy
R1262 T2011 T2010 nmod LSM,Pascal
R1263 T2012 T2010 nummod 5,Pascal
R1264 T2013 T2010 cc or,Pascal
R1265 T2014 T2015 nmod LSM,Meta
R1266 T2015 T2010 conj Meta,Pascal
R1267 T2016 T2015 nummod 510,Meta
R1268 T2017 T2010 punct ", ",Pascal
R1269 T2018 T2019 compound Carl,Zeiss
R1270 T2019 T2010 npadvmod Zeiss,Pascal
R1271 T2020 T2010 punct ", ",Pascal
R1272 T2021 T2010 npadvmod Jena,Pascal
R1273 T2022 T2021 cc or,Jena
R1274 T2023 T2021 conj Göttingen,Jena
R1275 T2024 T2010 punct ", ",Pascal
R1276 T2025 T2010 npadvmod Germany,Pascal
R1277 T2026 T2010 punct ),Pascal
R1278 T2027 T1982 punct .,washed
R1279 T2029 T2030 compound Laser,scanning
R1280 T2030 T2031 compound scanning,images
R1281 T2031 T2033 nsubjpass images,collected
R1282 T2032 T2031 compound brightfield,images
R1283 T2034 T2033 auxpass were,collected
R1284 T2035 T2036 aux to,document
R1285 T2036 T2033 advcl document,collected
R1286 T2037 T2038 amod structural,preservation
R1287 T2038 T2036 dobj preservation,document
R1288 T2039 T2033 punct ", ",collected
R1289 T2040 T2033 prep for,collected
R1290 T2041 T2042 amod morphometric,analysis
R1291 T2042 T2040 pobj analysis,for
R1292 T2043 T2040 cc and,for
R1293 T2044 T2040 conj for,for
R1294 T2045 T2044 pobj analysis,for
R1295 T2046 T2045 prep of,analysis
R1296 T2047 T2046 pobj pigmentation,of
R1297 T2048 T2033 punct .,collected
R1298 T2210 T2211 amod Quantitative,PCR
R1299 T2212 T2211 compound RT,PCR
R1300 T2213 T2211 punct -,PCR
R1301 T2215 T2216 nsubjpass Animals,anesthetized
R1302 T2217 T2216 auxpass were,anesthetized
R1303 T2218 T2216 advmod deeply,anesthetized
R1304 T2219 T2216 prep with,anesthetized
R1305 T2220 T2221 compound sodium,pentobarbital
R1306 T2221 T2219 pobj pentobarbital,with
R1307 T2222 T2223 punct (,mg
R1308 T2223 T2221 parataxis mg,pentobarbital
R1309 T2224 T2223 nummod 100,mg
R1310 T2225 T2226 punct /,kg
R1311 T2226 T2223 prep kg,mg
R1312 T2227 T2223 advmod i.p.,mg
R1313 T2228 T2223 punct ),mg
R1314 T2229 T2216 punct .,anesthetized
R1315 T2231 T2232 nsubjpass Kidneys,removed
R1316 T2233 T2231 cc and,Kidneys
R1317 T2234 T2231 conj brain,Kidneys
R1318 T2235 T2232 auxpass were,removed
R1319 T2236 T2232 cc and,removed
R1320 T2237 T2232 conj pulverized,removed
R1321 T2238 T2237 prep in,pulverized
R1322 T2239 T2240 compound liquid,N2
R1323 T2240 T2238 pobj N2,in
R1324 T2241 T2232 punct .,removed
R1325 T2243 T2244 amod Temporal,bones
R1326 T2244 T2245 nsubjpass bones,removed
R1327 T2246 T2245 auxpass were,removed
R1328 T2247 T2245 cc and,removed
R1329 T2248 T2249 nummod two,preparations
R1330 T2249 T2250 nsubjpass preparations,obtained
R1331 T2250 T2245 conj obtained,removed
R1332 T2251 T2249 prep of,preparations
R1333 T2252 T2253 amod inner,tissues
R1334 T2253 T2251 pobj tissues,of
R1335 T2254 T2253 compound ear,tissues
R1336 T2255 T2250 auxpass were,obtained
R1337 T2256 T2250 prep by,obtained
R1338 T2257 T2256 pobj microdissection,by
R1339 T2258 T2250 punct : ,obtained
R1340 T2259 T2260 punct (,1
R1341 T2260 T2261 meta 1,vascularis
R1342 T2261 T2250 dobj vascularis,obtained
R1343 T2262 T2260 punct ),1
R1344 T2263 T2261 compound stria,vascularis
R1345 T2264 T2261 prep without,vascularis
R1346 T2265 T2266 amod spiral,ligament
R1347 T2266 T2264 pobj ligament,without
R1348 T2267 T2261 punct ", ",vascularis
R1349 T2268 T2261 cc and,vascularis
R1350 T2269 T2270 punct (,2
R1351 T2270 T2271 meta 2,ganglia
R1352 T2271 T2261 conj ganglia,vascularis
R1353 T2272 T2270 punct ),2
R1354 T2273 T2271 amod spiral,ganglia
R1355 T2274 T2271 prep including,ganglia
R1356 T2275 T2276 det the,bone
R1357 T2276 T2274 pobj bone,including
R1358 T2277 T2276 amod surrounding,bone
R1359 T2278 T2276 cc and,bone
R1360 T2279 T2280 det the,organ
R1361 T2280 T2276 conj organ,bone
R1362 T2281 T2280 prep of,organ
R1363 T2282 T2281 pobj Corti,of
R1364 T2283 T2250 punct .,obtained
R1365 T2285 T2286 det The,medium
R1366 T2286 T2288 nsubjpass medium,changed
R1367 T2287 T2286 compound dissection,medium
R1368 T2289 T2288 auxpass was,changed
R1369 T2290 T2288 advmod twice,changed
R1370 T2291 T2288 prep during,changed
R1371 T2292 T2293 det the,microdissection
R1372 T2293 T2291 pobj microdissection,during
R1373 T2294 T2288 cc and,changed
R1374 T2295 T2296 amod isolated,tissues
R1375 T2296 T2297 nsubjpass tissues,washed
R1376 T2297 T2288 conj washed,changed
R1377 T2298 T2297 auxpass were,washed
R1378 T2299 T2300 nummod three,times
R1379 T2300 T2297 npadvmod times,washed
R1380 T2301 T2302 aux to,minimize
R1381 T2302 T2297 advcl minimize,washed
R1382 T2303 T2302 dobj cross-contamination,minimize
R1383 T2304 T2297 punct .,washed
R1384 T2306 T2307 det The,genotype
R1385 T2307 T2312 nsubjpass genotype,verified
R1386 T2308 T2307 nmod Slc26a4,genotype
R1387 T2309 T2308 punct -,Slc26a4
R1388 T2310 T2308 punct /,Slc26a4
R1389 T2311 T2308 punct -,Slc26a4
R1390 T2313 T2312 auxpass was,verified
R1391 T2314 T2312 prep by,verified
R1392 T2315 T2316 det the,observation
R1393 T2316 T2314 pobj observation,by
R1394 T2317 T2316 prep of,observation
R1395 T2318 T2319 amod large,otoconia
R1396 T2319 T2317 pobj otoconia,of
R1397 T2320 T2316 prep in,observation
R1398 T2321 T2322 det the,macula
R1399 T2322 T2320 pobj macula,in
R1400 T2323 T2322 amod utricular,macula
R1401 T2324 T2325 punct [,14
R1402 T2325 T2312 parataxis 14,verified
R1403 T2326 T2325 punct ],14
R1404 T2327 T2312 punct .,verified
R1405 T2329 T2330 amod Total,RNA
R1406 T2330 T2331 nsubjpass RNA,isolated
R1407 T2332 T2331 auxpass was,isolated
R1408 T2333 T2331 cc and,isolated
R1409 T2334 T2335 amod residual,contamination
R1410 T2335 T2337 nsubjpass contamination,removed
R1411 T2336 T2335 compound DNA,contamination
R1412 T2337 T2331 conj removed,isolated
R1413 T2338 T2337 auxpass was,removed
R1414 T2339 T2337 prep by,removed
R1415 T2340 T2341 amod enzymatic,digestion
R1416 T2341 T2339 pobj digestion,by
R1417 T2342 T2343 punct (,micro
R1418 T2343 T2337 parataxis micro,removed
R1419 T2344 T2343 compound RNeasy,micro
R1420 T2345 T2343 punct ", ",micro
R1421 T2346 T2343 npadvmod Qiagen,micro
R1422 T2347 T2343 punct ", ",micro
R1423 T2348 T2343 npadvmod Valencia,micro
R1424 T2349 T2343 punct ", ",micro
R1425 T2350 T2343 npadvmod CA,micro
R1426 T2351 T2343 punct ),micro
R1427 T2352 T2337 punct .,removed
R1428 T2354 T2355 nsubjpass Quality,determined
R1429 T2356 T2354 cc and,Quality
R1430 T2357 T2354 conj quantity,Quality
R1431 T2358 T2354 prep of,Quality
R1432 T2359 T2360 compound 18S,rRNA
R1433 T2360 T2358 pobj rRNA,of
R1434 T2361 T2360 acl obtained,rRNA
R1435 T2362 T2361 prep from,obtained
R1436 T2363 T2362 pobj kidneys,from
R1437 T2364 T2363 cc and,kidneys
R1438 T2365 T2363 conj brain,kidneys
R1439 T2366 T2355 auxpass were,determined
R1440 T2367 T2368 punct (,Assay
R1441 T2368 T2355 parataxis Assay,determined
R1442 T2369 T2368 compound Nano,Assay
R1443 T2370 T2368 punct ", ",Assay
R1444 T2371 T2372 nummod 2100,Bioanalyzer
R1445 T2372 T2368 appos Bioanalyzer,Assay
R1446 T2373 T2368 punct ", ",Assay
R1447 T2374 T2368 npadvmod Agilent,Assay
R1448 T2375 T2368 punct ", ",Assay
R1449 T2376 T2377 compound Palo,Alto
R1450 T2377 T2368 npadvmod Alto,Assay
R1451 T2378 T2368 punct ", ",Assay
R1452 T2379 T2368 npadvmod CA,Assay
R1453 T2380 T2368 punct ),Assay
R1454 T2381 T2355 punct .,determined
R1455 T2383 T2384 advmod Further,assessed
R1456 T2385 T2384 punct ", ",assessed
R1457 T2386 T2387 det the,quality
R1458 T2387 T2384 nsubjpass quality,assessed
R1459 T2388 T2387 prep of,quality
R1460 T2389 T2390 compound RNA,preparations
R1461 T2390 T2388 pobj preparations,of
R1462 T2391 T2390 acl obtained,preparations
R1463 T2392 T2391 prep from,obtained
R1464 T2393 T2394 compound stria,vascularis
R1465 T2394 T2392 pobj vascularis,from
R1466 T2395 T2394 cc and,vascularis
R1467 T2396 T2397 amod spiral,ganglia
R1468 T2397 T2394 conj ganglia,vascularis
R1469 T2398 T2384 auxpass was,assessed
R1470 T2399 T2400 punct (,Assay
R1471 T2400 T2384 parataxis Assay,assessed
R1472 T2401 T2400 compound Pico,Assay
R1473 T2402 T2400 punct ", ",Assay
R1474 T2403 T2404 nummod 2100,Bioanalyzer
R1475 T2404 T2400 appos Bioanalyzer,Assay
R1476 T2405 T2400 punct ),Assay
R1477 T2406 T2384 punct .,assessed
R1478 T2408 T2409 amod Real,time
R1479 T2409 T2410 compound time,PCR
R1480 T2410 T2413 nsubjpass PCR,performed
R1481 T2411 T2410 compound RT,PCR
R1482 T2412 T2410 punct -,PCR
R1483 T2414 T2413 auxpass was,performed
R1484 T2415 T2413 prep on,performed
R1485 T2416 T2415 pobj RNA,on
R1486 T2417 T2416 acl obtained,RNA
R1487 T2418 T2417 prep from,obtained
R1488 T2419 T2420 amod individual,animals
R1489 T2420 T2418 pobj animals,from
R1490 T2421 T2422 punct (,Cycler
R1491 T2422 T2417 parataxis Cycler,obtained
R1492 T2423 T2424 compound OneStep,PCR
R1493 T2424 T2422 dep PCR,Cycler
R1494 T2425 T2424 compound RT,PCR
R1495 T2426 T2424 punct -,PCR
R1496 T2427 T2424 punct ", ",PCR
R1497 T2428 T2424 npadvmod Qiagen,PCR
R1498 T2429 T2422 punct ;,Cycler
R1499 T2430 T2422 compound Smart,Cycler
R1500 T2431 T2422 punct ", ",Cycler
R1501 T2432 T2422 npadvmod Cepheid,Cycler
R1502 T2433 T2422 punct ", ",Cycler
R1503 T2434 T2422 npadvmod Sunnyvale,Cycler
R1504 T2435 T2422 punct ", ",Cycler
R1505 T2436 T2422 npadvmod CA,Cycler
R1506 T2437 T2422 punct ),Cycler
R1507 T2438 T2413 prep in,performed
R1508 T2439 T2440 det the,presence
R1509 T2440 T2438 pobj presence,in
R1510 T2441 T2440 prep of,presence
R1511 T2442 T2443 nummod 0.2,SYBR
R1512 T2443 T2445 nmod SYBR,I
R1513 T2444 T2443 punct ×,SYBR
R1514 T2445 T2441 pobj I,of
R1515 T2446 T2445 amod green,I
R1516 T2447 T2448 punct (,Probes
R1517 T2448 T2445 parataxis Probes,I
R1518 T2449 T2448 compound Molecular,Probes
R1519 T2450 T2448 punct ),Probes
R1520 T2451 T2413 punct .,performed
R1521 T2453 T2454 nsubjpass Transcripts,amplified
R1522 T2455 T2453 prep of,Transcripts
R1523 T2456 T2457 compound 18S,rRNA
R1524 T2457 T2455 pobj rRNA,of
R1525 T2458 T2457 cc and,rRNA
R1526 T2459 T2457 conj mRNA,rRNA
R1527 T2460 T2453 prep for,Transcripts
R1528 T2461 T2462 det the,channels
R1529 T2462 T2460 pobj channels,for
R1530 T2463 T2462 nmod K,channels
R1531 T2464 T2463 punct +,K
R1532 T2465 T2462 appos KCNJ10,channels
R1533 T2466 T2465 punct ", ",KCNJ10
R1534 T2467 T2465 conj KCNQ1,KCNJ10
R1535 T2468 T2467 cc and,KCNQ1
R1536 T2469 T2467 conj KCNQ4,KCNQ1
R1537 T2470 T2454 auxpass were,amplified
R1538 T2471 T2454 advcl using,amplified
R1539 T2472 T2473 npadvmod gene,specific
R1540 T2473 T2475 amod specific,primers
R1541 T2474 T2473 punct -,specific
R1542 T2475 T2471 dobj primers,using
R1543 T2476 T2477 punct (,Table
R1544 T2477 T2454 parataxis Table,amplified
R1545 T2478 T2477 nummod 1,Table
R1546 T2479 T2477 punct ),Table
R1547 T2480 T2454 punct .,amplified
R1548 T2482 T2483 nsubjpass RT,performed
R1549 T2484 T2483 auxpass was,performed
R1550 T2485 T2483 prep for,performed
R1551 T2486 T2487 nummod 30,min
R1552 T2487 T2485 pobj min,for
R1553 T2488 T2483 prep at,performed
R1554 T2489 T2490 nummod 50,°C
R1555 T2490 T2488 pobj °C,at
R1556 T2491 T2483 cc and,performed
R1557 T2492 T2493 nummod 15,min
R1558 T2493 T2483 conj min,performed
R1559 T2494 T2493 prep at,min
R1560 T2495 T2496 nummod 95,°C
R1561 T2496 T2494 pobj °C,at
R1562 T2497 T2483 punct .,performed
R1563 T2499 T2500 nsubj PCR,consisted
R1564 T2501 T2500 prep of,consisted
R1565 T2502 T2503 nummod 50,cycles
R1566 T2503 T2501 pobj cycles,of
R1567 T2504 T2503 prep of,cycles
R1568 T2505 T2506 nummod 1,min
R1569 T2506 T2504 pobj min,of
R1570 T2507 T2506 prep at,min
R1571 T2508 T2509 nummod 60,°C
R1572 T2509 T2507 pobj °C,at
R1573 T2510 T2506 punct ", ",min
R1574 T2511 T2512 nummod 1,min
R1575 T2512 T2506 conj min,min
R1576 T2513 T2512 prep at,min
R1577 T2514 T2515 nummod 72,°C
R1578 T2515 T2513 pobj °C,at
R1579 T2516 T2512 punct ", ",min
R1580 T2517 T2512 conj 7s,min
R1581 T2518 T2517 amod heating,7s
R1582 T2519 T2517 prep to,7s
R1583 T2520 T2521 amod hot,measurement
R1584 T2521 T2523 compound measurement,temperature
R1585 T2522 T2521 punct -,measurement
R1586 T2523 T2519 pobj temperature,to
R1587 T2524 T2517 punct ", ",7s
R1588 T2525 T2526 compound 13s,measurement
R1589 T2526 T2517 conj measurement,7s
R1590 T2527 T2526 compound hot,measurement
R1591 T2528 T2526 punct -,measurement
R1592 T2529 T2526 prep at,measurement
R1593 T2530 T2531 quantmod 3,5
R1594 T2531 T2533 nummod 5,°C
R1595 T2532 T2531 punct –,5
R1596 T2533 T2534 npadvmod °C,below
R1597 T2534 T2529 prep below,at
R1598 T2535 T2536 compound product,temperature
R1599 T2536 T2534 pobj temperature,below
R1600 T2537 T2536 compound melting,temperature
R1601 T2538 T2539 punct (,Table
R1602 T2539 T2536 parataxis Table,temperature
R1603 T2540 T2539 nummod 1,Table
R1604 T2541 T2539 punct ),Table
R1605 T2542 T2526 cc and,measurement
R1606 T2543 T2544 nummod 1,min
R1607 T2544 T2526 conj min,measurement
R1608 T2545 T2544 prep at,min
R1609 T2546 T2547 nummod 94,°C
R1610 T2547 T2545 pobj °C,at
R1611 T2548 T2500 punct .,consisted
R1612 T2550 T2551 amod Hot,measurements
R1613 T2551 T2553 nsubjpass measurements,performed
R1614 T2552 T2551 punct -,measurements
R1615 T2554 T2553 auxpass were,performed
R1616 T2555 T2556 aux to,eliminate
R1617 T2556 T2553 advcl eliminate,performed
R1618 T2557 T2558 det the,detection
R1619 T2558 T2556 dobj detection,eliminate
R1620 T2559 T2558 prep of,detection
R1621 T2560 T2561 compound primer,dimers
R1622 T2561 T2559 pobj dimers,of
R1623 T2562 T2561 punct -,dimers
R1624 T2563 T2564 dep that,had
R1625 T2564 T2561 relcl had,dimers
R1626 T2565 T2566 compound melting,temperatures
R1627 T2566 T2564 dobj temperatures,had
R1628 T2567 T2564 prep between,had
R1629 T2568 T2569 nummod 72,°C
R1630 T2569 T2567 pobj °C,between
R1631 T2570 T2568 cc and,72
R1632 T2571 T2568 conj 75,72
R1633 T2572 T2573 punct [,15
R1634 T2573 T2564 parataxis 15,had
R1635 T2574 T2573 punct ],15
R1636 T2575 T2553 punct .,performed
R1637 T2577 T2578 nsubjpass PCR,followed
R1638 T2579 T2578 auxpass was,followed
R1639 T2580 T2578 agent by,followed
R1640 T2581 T2582 det a,melt
R1641 T2582 T2580 pobj melt,by
R1642 T2583 T2584 punct (,°C
R1643 T2584 T2582 parataxis °C,melt
R1644 T2585 T2586 quantmod 60,95
R1645 T2586 T2584 nummod 95,°C
R1646 T2587 T2586 quantmod to,95
R1647 T2588 T2584 punct ),°C
R1648 T2589 T2578 punct .,followed
R1649 T2591 T2592 det The,generation
R1650 T2592 T2593 nsubjpass generation,checked
R1651 T2594 T2592 prep of,generation
R1652 T2595 T2596 det a,product
R1653 T2596 T2594 pobj product,of
R1654 T2597 T2596 amod single,product
R1655 T2598 T2596 prep of,product
R1656 T2599 T2600 amod appropriate,size
R1657 T2600 T2598 pobj size,of
R1658 T2601 T2593 auxpass was,checked
R1659 T2602 T2593 advmod routinely,checked
R1660 T2603 T2593 prep by,checked
R1661 T2604 T2605 det the,presence
R1662 T2605 T2603 pobj presence,by
R1663 T2606 T2605 prep of,presence
R1664 T2607 T2608 det a,peak
R1665 T2608 T2606 pobj peak,of
R1666 T2609 T2608 amod single,peak
R1667 T2610 T2608 compound melt,peak
R1668 T2611 T2603 cc and,by
R1669 T2612 T2603 conj by,by
R1670 T2613 T2614 compound agarose,gel
R1671 T2614 T2615 compound gel,electrophoresis
R1673 T2616 T2615 punct -,electrophoresis
R1674 T2617 T2593 punct .,checked
R1675 T2619 T2620 compound Product,identity
R1676 T2620 T2621 nsubjpass identity,confirmed
R1677 T2622 T2621 auxpass was,confirmed
R1678 T2623 T2621 prep by,confirmed
R1679 T2624 T2623 pobj sequencing,by
R1680 T2625 T2621 punct .,confirmed
R1681 T2627 T2628 compound "Table 1 Primers Template",molecules
R1682 T2628 T2629 nsubjpass molecules,quantified
R1683 T2630 T2628 punct (,molecules
R1684 T2631 T2628 appos T,molecules
R1685 T2632 T2628 punct ),molecules
R1686 T2633 T2629 auxpass were,quantified
R1687 T2634 T2629 prep according,quantified
R1688 T2635 T2634 prep to,according
R1689 T2636 T2637 nsubj T,P
R1690 T2637 T2635 pcomp P,to
R1691 T2638 T2637 punct =,P
R1692 T2639 T2640 punct /,Ct
R1693 T2640 T2637 prep Ct,P
R1694 T2641 T2640 punct (,Ct
R1695 T2642 T2640 nmod F,Ct
R1696 T2643 T2640 punct ^,Ct
R1697 T2644 T2640 punct ),Ct
R1698 T2645 T2646 advmod where,is
R1699 T2646 T2637 advcl is,P
R1700 T2647 T2646 nsubj P,is
R1701 T2648 T2649 det the,number
R1702 T2649 T2646 attr number,is
R1703 T2650 T2649 prep of,number
R1704 T2651 T2652 compound product,molecules
R1705 T2652 T2650 pobj molecules,of
R1706 T2653 T2646 punct ", ",is
R1707 T2654 T2655 nsubj F,is
R1708 T2655 T2646 conj is,is
R1709 T2656 T2657 det the,fidelity
R1710 T2657 T2655 attr fidelity,is
R1711 T2658 T2657 prep of,fidelity
R1712 T2659 T2660 det the,reaction
R1713 T2660 T2658 pobj reaction,of
R1714 T2661 T2655 cc and,is
R1715 T2662 T2663 nsubj Ct,is
R1716 T2663 T2655 conj is,is
R1717 T2664 T2665 det the,cycle
R1718 T2665 T2663 attr cycle,is
R1719 T2666 T2667 prep at,reaches
R1720 T2667 T2665 relcl reaches,cycle
R1721 T2668 T2666 pobj which,at
R1722 T2669 T2670 det the,number
R1723 T2670 T2667 nsubj number,reaches
R1724 T2671 T2670 prep of,number
R1725 T2672 T2673 compound product,molecules
R1726 T2673 T2671 pobj molecules,of
R1727 T2674 T2675 det a,threshold
R1728 T2675 T2667 dobj threshold,reaches
R1729 T2676 T2675 amod chosen,threshold
R1730 T2677 T2678 punct [,15
R1731 T2678 T2646 parataxis 15,is
R1732 T2679 T2678 punct ],15
R1733 T2680 T2629 punct .,quantified
R1734 T2682 T2683 nsubjpass Fidelity,obtained
R1735 T2684 T2682 punct (,Fidelity
R1736 T2685 T2682 appos F,Fidelity
R1737 T2686 T2682 punct ),Fidelity
R1738 T2687 T2683 auxpass was,obtained
R1739 T2688 T2683 prep from,obtained
R1740 T2689 T2690 det the,slope
R1741 T2690 T2688 pobj slope,from
R1742 T2691 T2690 punct (,slope
R1743 T2692 T2690 appos S,slope
R1744 T2693 T2690 punct ),slope
R1745 T2694 T2690 prep of,slope
R1746 T2695 T2696 det the,phase
R1747 T2696 T2694 pobj phase,of
R1748 T2697 T2698 npadvmod log,linear
R1749 T2698 T2696 amod linear,phase
R1750 T2699 T2698 punct -,linear
R1751 T2700 T2696 prep of,phase
R1752 T2701 T2702 det the,curve
R1753 T2702 T2700 pobj curve,of
R1754 T2703 T2702 compound growth,curve
R1755 T2704 T2683 prep via,obtained
R1756 T2705 T2706 det a,polynomial
R1757 T2706 T2704 pobj polynomial,via
R1758 T2707 T2708 amod best,fit
R1759 T2708 T2706 nmod fit,polynomial
R1760 T2709 T2708 punct -,fit
R1761 T2710 T2711 amod fifth,order
R1762 T2711 T2706 compound order,polynomial
R1763 T2712 T2711 punct -,order
R1764 T2713 T2706 punct : ,polynomial
R1765 T2714 T2715 nsubj F,7.39
R1766 T2715 T2706 appos 7.39,polynomial
R1767 T2716 T2715 punct =,7.39
R1768 T2717 T2715 punct +,7.39
R1769 T2718 T2719 nummod 3.80,S
R1770 T2719 T2715 appos S,7.39
R1771 T2720 T2719 punct ×,S
R1772 T2721 T2715 punct +,7.39
R1773 T2722 T2723 nummod 1.05,S2
R1774 T2723 T2715 appos S2,7.39
R1775 T2724 T2723 punct ×,S2
R1776 T2725 T2715 punct +,7.39
R1777 T2726 T2727 nummod 0.15,S3
R1778 T2727 T2715 appos S3,7.39
R1779 T2728 T2727 punct ×,S3
R1780 T2729 T2715 punct +,7.39
R1781 T2730 T2731 quantmod 11.38,3
R1782 T2731 T2735 nummod 3,S4
R1783 T2732 T2731 punct ×,3
R1784 T2733 T2731 compound 10,3
R1785 T2734 T2731 punct -,3
R1786 T2735 T2715 appos S4,7.39
R1787 T2736 T2735 punct *,S4
R1788 T2737 T2715 punct +,7.39
R1789 T2738 T2739 quantmod 3.39,4
R1790 T2739 T2743 nummod 4,S5
R1791 T2740 T2739 punct ×,4
R1792 T2741 T2739 compound 10,4
R1793 T2742 T2739 punct -,4
R1794 T2743 T2715 appos S5,7.39
R1795 T2744 T2743 punct ×,S5
R1796 T2745 T2683 punct .,obtained
R1797 T2747 T2748 det The,number
R1798 T2748 T2749 nsubjpass number,determined
R1799 T2750 T2748 prep of,number
R1800 T2751 T2752 compound product,molecules
R1801 T2752 T2750 pobj molecules,of
R1802 T2753 T2752 prep at,molecules
R1803 T2754 T2753 pobj threshold,at
R1804 T2755 T2752 punct (,molecules
R1805 T2756 T2752 appos PCt,molecules
R1806 T2757 T2752 punct ),molecules
R1807 T2758 T2749 auxpass was,determined
R1808 T2759 T2749 prep by,determined
R1809 T2760 T2759 pobj amplification,by
R1810 T2761 T2760 prep of,amplification
R1811 T2762 T2763 amod known,amounts
R1812 T2763 T2761 pobj amounts,of
R1813 T2764 T2763 prep of,amounts
R1814 T2765 T2766 compound 18S,rRNA
R1815 T2766 T2764 pobj rRNA,of
R1816 T2767 T2760 prep according,amplification
R1817 T2768 T2767 prep to,according
R1818 T2769 T2770 nsubj PCt,T
R1819 T2770 T2768 pcomp T,to
R1820 T2771 T2770 punct =,T
R1821 T2772 T2770 punct ×,T
R1822 T2773 T2774 nmod F,Ct
R1823 T2774 T2770 appos Ct,T
R1824 T2775 T2774 punct ^,Ct
R1825 T2776 T2749 punct .,determined
R1826 T2778 T2779 nsubjpass Quantifications,used
R1827 T2780 T2778 prep of,Quantifications
R1828 T2781 T2782 compound 18S,rRNA
R1829 T2782 T2780 pobj rRNA,of
R1830 T2783 T2779 auxpass were,used
R1831 T2784 T2785 aux to,compare
R1832 T2785 T2779 advcl compare,used
R1833 T2786 T2787 compound tissue,amounts
R1834 T2787 T2785 dobj amounts,compare
R1835 T2788 T2779 punct .,used
R1836 T2790 T2791 amod Genomic,contamination
R1837 T2791 T2792 nsubjpass contamination,assessed
R1838 T2793 T2791 prep of,contamination
R1839 T2794 T2795 amod inner,ear
R1840 T2795 T2796 compound ear,samples
R1841 T2796 T2793 pobj samples,of
R1842 T2797 T2792 auxpass was,assessed
R1843 T2798 T2799 aux to,be
R1844 T2799 T2792 xcomp be,assessed
R1845 T2800 T2801 punct <,%
R1846 T2801 T2799 acomp %,be
R1847 T2802 T2801 nummod 0.02,%
R1848 T2803 T2792 prep by,assessed
R1849 T2804 T2803 pobj omission,by
R1850 T2805 T2804 prep of,omission
R1851 T2806 T2807 det the,step
R1852 T2807 T2805 pobj step,of
R1853 T2808 T2807 compound RT,step
R1854 T2809 T2792 punct .,assessed
R1855 T2833 T2834 advmod In,vitro
R1856 T2834 T2835 amod vitro,electrophysiology
R1857 T2837 T2838 nsubjpass Animals,anesthetized
R1858 T2839 T2838 auxpass were,anesthetized
R1859 T2840 T2838 advmod deeply,anesthetized
R1860 T2841 T2838 prep with,anesthetized
R1861 T2842 T2843 compound sodium,pentobarbital
R1862 T2843 T2841 pobj pentobarbital,with
R1863 T2844 T2845 punct (,mg
R1864 T2845 T2838 parataxis mg,anesthetized
R1865 T2846 T2845 nummod 100,mg
R1866 T2847 T2848 punct /,kg
R1867 T2848 T2845 prep kg,mg
R1868 T2849 T2845 advmod i.p.,mg
R1869 T2850 T2845 punct ),mg
R1870 T2851 T2838 punct .,anesthetized
R1871 T2853 T2854 compound Stria,vascularis
R1872 T2854 T2855 nsubjpass vascularis,obtained
R1873 T2856 T2854 prep without,vascularis
R1874 T2857 T2858 amod spiral,ligament
R1875 T2858 T2856 pobj ligament,without
R1876 T2859 T2855 auxpass was,obtained
R1877 T2860 T2855 prep by,obtained
R1878 T2861 T2860 pobj microdissection,by
R1879 T2862 T2855 punct .,obtained
R1880 T2864 T2865 nsubjpass Currents,recorded
R1881 T2866 T2864 acl generated,Currents
R1882 T2867 T2866 agent by,generated
R1883 T2868 T2869 det the,epithelium
R1884 T2869 T2867 pobj epithelium,by
R1885 T2870 T2869 nmod stria,epithelium
R1886 T2871 T2869 amod marginal,epithelium
R1887 T2872 T2865 auxpass were,recorded
R1888 T2873 T2874 punct [,16
R1889 T2874 T2865 parataxis 16,recorded
R1890 T2875 T2874 punct ],16
R1891 T2876 T2865 punct .,recorded
R1892 T2878 T2879 det A,microelectrode
R1893 T2879 T2884 nsubjpass microelectrode,positioned
R1894 T2880 T2881 compound Pt,Ir
R1895 T2881 T2879 compound Ir,microelectrode
R1896 T2882 T2881 punct -,Ir
R1897 T2883 T2879 compound wire,microelectrode
R1898 T2885 T2879 prep with,microelectrode
R1899 T2886 T2887 det a,tip
R1900 T2887 T2885 pobj tip,with
R1901 T2888 T2887 nmod Pt,tip
R1902 T2889 T2888 punct -,Pt
R1903 T2890 T2888 amod black,Pt
R1904 T2891 T2884 auxpass was,positioned
R1905 T2892 T2893 compound 20,30
R1906 T2893 T2895 nummod 30,μm
R1907 T2894 T2893 punct –,30
R1908 T2895 T2896 npadvmod μm,from
R1909 T2896 T2884 prep from,positioned
R1910 T2897 T2898 det the,surface
R1911 T2898 T2896 pobj surface,from
R1912 T2899 T2898 amod apical,surface
R1913 T2900 T2898 prep of,surface
R1914 T2901 T2902 det the,epithelium
R1915 T2902 T2900 pobj epithelium,of
R1916 T2903 T2884 cc and,positioned
R1917 T2904 T2884 conj vibrated,positioned
R1918 T2905 T2904 prep at,vibrated
R1919 T2906 T2907 compound 200,400
R1920 T2907 T2909 nummod 400,Hz
R1921 T2908 T2907 punct –,400
R1922 T2909 T2905 pobj Hz,at
R1923 T2910 T2904 agent by,vibrated
R1924 T2911 T2912 amod piezo,electric
R1925 T2912 T2914 amod electric,elements
R1926 T2913 T2912 punct -,electric
R1927 T2914 T2910 pobj elements,by
R1928 T2915 T2914 compound bimorph,elements
R1929 T2916 T2917 punct (,version
R1930 T2917 T2914 parataxis version,elements
R1931 T2918 T2919 compound Applicable,Electronics
R1932 T2919 T2917 dep Electronics,version
R1933 T2920 T2919 punct ", ",Electronics
R1934 T2921 T2922 compound Forest,Dale
R1935 T2922 T2919 npadvmod Dale,Electronics
R1936 T2923 T2919 punct ", ",Electronics
R1937 T2924 T2919 npadvmod MA,Electronics
R1938 T2925 T2917 punct ;,version
R1939 T2926 T2917 compound ASET,version
R1940 T2927 T2917 nummod 1.05,version
R1941 T2928 T2917 punct ", ",version
R1942 T2929 T2930 compound Science,Wares
R1943 T2930 T2917 npadvmod Wares,version
R1944 T2931 T2917 punct ", ",version
R1945 T2932 T2933 compound East,Falmouth
R1946 T2933 T2917 npadvmod Falmouth,version
R1947 T2934 T2917 punct ", ",version
R1948 T2935 T2917 npadvmod MA,version
R1949 T2936 T2917 punct ),version
R1950 T2937 T2884 punct .,positioned
R1951 T2939 T2940 det A,electrode
R1952 T2940 T2944 nsubj electrode,served
R1953 T2941 T2940 nmod Pt,electrode
R1954 T2942 T2941 punct -,Pt
R1955 T2943 T2941 amod black,Pt
R1956 T2945 T2946 punct (,gauge
R1957 T2946 T2940 parataxis gauge,electrode
R1958 T2947 T2946 nummod 26,gauge
R1959 T2948 T2946 punct -,gauge
R1960 T2949 T2946 punct ),gauge
R1961 T2950 T2944 prep as,served
R1962 T2951 T2950 pobj reference,as
R1963 T2952 T2944 prep in,served
R1964 T2953 T2954 det the,chamber
R1965 T2954 T2952 pobj chamber,in
R1966 T2955 T2954 compound bath,chamber
R1967 T2956 T2944 punct .,served
R1968 T2958 T2959 det The,signals
R1969 T2959 T2960 nsubjpass signals,digitized
R1970 T2961 T2959 prep from,signals
R1971 T2962 T2963 det the,detectors
R1972 T2963 T2961 pobj detectors,from
R1973 T2964 T2965 npadvmod phase,sensitive
R1974 T2965 T2963 amod sensitive,detectors
R1975 T2966 T2965 punct -,sensitive
R1976 T2967 T2960 auxpass were,digitized
R1977 T2968 T2969 punct (,bit
R1978 T2969 T2960 parataxis bit,digitized
R1979 T2970 T2969 nummod 16,bit
R1980 T2971 T2969 punct ),bit
R1981 T2972 T2960 prep at,digitized
R1982 T2973 T2974 det a,rate
R1983 T2974 T2972 pobj rate,at
R1984 T2975 T2974 prep of,rate
R1985 T2976 T2977 nummod 0.5,Hz
R1986 T2977 T2975 pobj Hz,of
R1987 T2978 T2960 punct .,digitized
R1988 T2980 T2981 det The,output
R1989 T2981 T2982 nsubjpass output,expressed
R1990 T2983 T2982 auxpass was,expressed
R1991 T2984 T2982 prep as,expressed
R1992 T2985 T2986 amod current,density
R1993 T2986 T2984 pobj density,as
R1994 T2987 T2986 prep at,density
R1995 T2988 T2989 det the,electrode
R1996 T2989 T2987 pobj electrode,at
R1997 T2990 T2982 punct .,expressed
R1998 T3050 T3051 advmod In,situ
R1999 T3051 T3052 amod situ,electrophysiology
R2000 T3054 T3055 nsubjpass Animals,anesthetized
R2001 T3056 T3055 auxpass were,anesthetized
R2002 T3057 T3055 prep with,anesthetized
R2003 T3058 T3057 pobj inactin,with
R2004 T3059 T3060 punct (,mg
R2005 T3060 T3058 parataxis mg,inactin
R2006 T3061 T3062 compound thiobutabarbital,salt
R2007 T3062 T3060 dep salt,mg
R2008 T3063 T3062 compound sodium,salt
R2009 T3064 T3060 punct ", ",mg
R2010 T3065 T3060 nummod 140,mg
R2011 T3066 T3067 punct /,kg
R2012 T3067 T3060 prep kg,mg
R2013 T3068 T3060 advmod ip,mg
R2014 T3069 T3060 punct ),mg
R2015 T3070 T3055 punct .,anesthetized
R2016 T3072 T3073 det The,potential
R2017 T3073 T3075 nsubjpass potential,measured
R2018 T3074 T3073 amod endocochlear,potential
R2019 T3076 T3073 cc and,potential
R2020 T3077 T3078 det the,K
R2021 T3078 T3073 conj K,potential
R2022 T3079 T3078 amod endolymphatic,K
R2023 T3080 T3078 punct [,K
R2024 T3081 T3078 punct +,K
R2025 T3082 T3078 punct ],K
R2026 T3083 T3075 auxpass were,measured
R2027 T3084 T3075 prep with,measured
R2028 T3085 T3086 amod double,barreled
R2029 T3086 T3088 amod barreled,microelectrodes
R2030 T3087 T3086 punct -,barreled
R2031 T3088 T3084 pobj microelectrodes,with
R2032 T3089 T3090 punct [,17
R2033 T3090 T3075 parataxis 17,measured
R2034 T3091 T3090 punct ],17
R2035 T3092 T3075 punct .,measured
R2036 T3094 T3095 nsubjpass Measurements,made
R2037 T3096 T3095 auxpass were,made
R2038 T3097 T3095 prep in,made
R2039 T3098 T3099 det the,turn
R2040 T3099 T3097 pobj turn,in
R2041 T3100 T3099 amod basal,turn
R2042 T3101 T3095 prep by,made
R2043 T3102 T3103 det a,approach
R2044 T3103 T3101 pobj approach,by
R2045 T3104 T3105 amod round,window
R2046 T3105 T3103 compound window,approach
R2047 T3106 T3105 punct -,window
R2048 T3107 T3103 prep through,approach
R2049 T3108 T3109 det the,membrane
R2050 T3109 T3107 pobj membrane,through
R2051 T3110 T3109 amod basilar,membrane
R2052 T3111 T3107 cc and,through
R2053 T3112 T3107 conj in,through
R2054 T3113 T3114 det the,turn
R2055 T3114 T3112 pobj turn,in
R2056 T3115 T3114 amod apical,turn
R2057 T3116 T3117 mark after,thinning
R2058 T3117 T3095 advcl thinning,made
R2059 T3118 T3119 det the,bone
R2060 T3119 T3117 dobj bone,thinning
R2061 T3120 T3117 prep over,thinning
R2062 T3121 T3122 det the,vascularis
R2063 T3122 T3120 pobj vascularis,over
R2064 T3123 T3122 compound stria,vascularis
R2065 T3124 T3117 cc and,thinning
R2066 T3125 T3117 conj picking,thinning
R2067 T3126 T3127 det a,hole
R2068 T3127 T3125 dobj hole,picking
R2069 T3128 T3127 amod small,hole
R2070 T3129 T3130 punct (,μm
R2071 T3130 T3127 parataxis μm,hole
R2072 T3131 T3132 punct ~,30
R2073 T3132 T3130 nummod 30,μm
R2074 T3133 T3130 punct ),μm
R2075 T3134 T3095 punct .,made
R2076 T3136 T3137 npadvmod K,selective
R2077 T3137 T3140 amod selective,electrodes
R2078 T3138 T3136 punct +,K
R2079 T3139 T3137 punct -,selective
R2080 T3140 T3141 nsubjpass electrodes,calibrated
R2081 T3142 T3141 auxpass were,calibrated
R2082 T3143 T3141 prep in,calibrated
R2083 T3144 T3143 pobj solutions,in
R2084 T3145 T3144 prep of,solutions
R2085 T3146 T3147 amod constant,concentration
R2086 T3147 T3145 pobj concentration,of
R2087 T3148 T3147 nmod cation,concentration
R2088 T3149 T3150 punct (,K
R2089 T3150 T3148 parataxis K,cation
R2090 T3151 T3150 punct +,K
R2091 T3152 T3150 cc and,K
R2092 T3153 T3150 conj Na,K
R2093 T3154 T3153 punct +,Na
R2094 T3155 T3150 punct ),K
R2095 T3156 T3144 prep of,solutions
R2096 T3157 T3158 nummod 150,mM
R2097 T3158 T3156 pobj mM,of
R2098 T3159 T3141 punct .,calibrated
R2099 T3161 T3162 det The,selectivity
R2100 T3162 T3164 nsubj selectivity,produced
R2101 T3163 T3162 amod minor,selectivity
R2102 T3165 T3162 prep of,selectivity
R2103 T3166 T3167 det the,electrodes
R2104 T3167 T3165 pobj electrodes,of
R2105 T3168 T3167 nmod K,electrodes
R2106 T3169 T3168 punct +,K
R2107 T3170 T3162 prep for,selectivity
R2108 T3171 T3170 pobj Na,for
R2109 T3172 T3171 punct +,Na
R2110 T3173 T3174 det a,nonlinearity
R2111 T3174 T3164 dobj nonlinearity,produced
R2112 T3175 T3174 prep in,nonlinearity
R2113 T3176 T3177 det the,curve
R2114 T3177 T3175 pobj curve,in
R2115 T3178 T3177 compound calibration,curve
R2116 T3179 T3174 punct ", ",nonlinearity
R2117 T3180 T3181 dep which,fitted
R2118 T3181 T3174 relcl fitted,nonlinearity
R2119 T3182 T3181 auxpass was,fitted
R2120 T3183 T3181 advmod closely,fitted
R2121 T3184 T3181 prep by,fitted
R2122 T3185 T3186 det the,equation
R2123 T3186 T3184 pobj equation,by
R2124 T3187 T3186 compound Nicolski,equation
R2125 T3188 T3181 advcl using,fitted
R2126 T3189 T3190 amod nonlinear,software
R2127 T3190 T3188 dobj software,using
R2128 T3191 T3190 nmod curve,software
R2129 T3192 T3191 punct -,curve
R2130 T3193 T3191 amod fitting,curve
R2131 T3194 T3195 punct (,OriginLab
R2132 T3195 T3190 parataxis OriginLab,software
R2133 T3196 T3195 punct ", ",OriginLab
R2134 T3197 T3195 npadvmod Northampton,OriginLab
R2135 T3198 T3195 punct ", ",OriginLab
R2136 T3199 T3195 npadvmod MA,OriginLab
R2137 T3200 T3195 punct ),OriginLab
R2138 T3201 T3174 punct : ,nonlinearity
R2139 T3202 T3203 nsubj V,K
R2140 T3203 T3174 appos K,nonlinearity
R2141 T3204 T3203 punct =,K
R2142 T3205 T3203 nmod Vi,K
R2143 T3206 T3203 punct +,K
R2144 T3207 T3203 nmod S,K
R2145 T3208 T3203 punct ×,K
R2146 T3209 T3203 nmod log,K
R2147 T3210 T3203 punct (,K
R2148 T3211 T3203 punct [,K
R2149 T3212 T3203 punct +,K
R2150 T3213 T3203 punct ],K
R2151 T3214 T3203 punct +,K
R2152 T3215 T3216 nmod A,Na
R2153 T3216 T3203 appos Na,K
R2154 T3217 T3216 punct ×,Na
R2155 T3218 T3216 punct [,Na
R2156 T3219 T3216 punct +,Na
R2157 T3220 T3203 punct ],K
R2158 T3221 T3203 punct ),K
R2159 T3222 T3203 punct ", ",K
R2160 T3223 T3224 advmod where,is
R2161 T3224 T3203 advcl is,K
R2162 T3225 T3224 nsubj Vi,is
R2163 T3226 T3227 det an,term
R2164 T3227 T3224 attr term,is
R2165 T3228 T3227 amod offset,term
R2166 T3229 T3224 punct ", ",is
R2167 T3230 T3231 nsubj S,is
R2168 T3231 T3224 conj is,is
R2169 T3232 T3231 attr slope,is
R2170 T3233 T3231 punct ", ",is
R2171 T3234 T3235 nsubj A,is
R2172 T3235 T3231 conj is,is
R2173 T3236 T3235 attr selectivity,is
R2174 T3237 T3235 punct ", ",is
R2175 T3238 T3235 cc and,is
R2176 T3239 T3240 punct [,Na
R2177 T3240 T3241 nsubj Na,is
R2178 T3241 T3235 conj is,is
R2179 T3242 T3240 punct +,Na
R2180 T3243 T3240 punct ],Na
R2181 T3244 T3245 nmod Na,concentration
R2182 T3245 T3241 attr concentration,is
R2183 T3246 T3244 punct +,Na
R2184 T3247 T3164 punct .,produced
R2185 T3249 T3250 nsubjpass Calibrations,made
R2186 T3251 T3250 auxpass were,made
R2187 T3252 T3253 advmod immediately,after
R2188 T3253 T3250 prep after,made
R2189 T3254 T3253 pobj withdrawal,after
R2190 T3255 T3254 prep of,withdrawal
R2191 T3256 T3257 det the,electrodes
R2192 T3257 T3255 pobj electrodes,of
R2193 T3258 T3254 prep from,withdrawal
R2194 T3259 T3260 det the,cochlea
R2195 T3260 T3258 pobj cochlea,from
R2196 T3261 T3250 punct .,made
R2197 T3263 T3264 nmod Plasma,concentrations
R2198 T3264 T3267 nsubjpass concentrations,obtained
R2199 T3265 T3264 nmod K,concentrations
R2200 T3266 T3265 punct +,K
R2201 T3268 T3267 auxpass were,obtained
R2202 T3269 T3267 advcl using,obtained
R2203 T3270 T3271 det a,analyzer
R2204 T3271 T3269 dobj analyzer,using
R2205 T3272 T3271 compound blood,analyzer
R2206 T3273 T3274 punct (,M
R2207 T3274 T3267 parataxis M,obtained
R2208 T3275 T3274 compound Stat,M
R2209 T3276 T3274 compound Profile,M
R2210 T3277 T3274 punct ", ",M
R2211 T3278 T3279 compound Nova,Biomedical
R2212 T3279 T3274 npadvmod Biomedical,M
R2213 T3280 T3274 punct ", ",M
R2214 T3281 T3274 npadvmod Waltham,M
R2215 T3282 T3274 punct ", ",M
R2216 T3283 T3274 npadvmod MA,M
R2217 T3284 T3274 punct ),M
R2218 T3285 T3267 punct .,obtained
R2219 T3287 T3288 nsubjpass Data,presented
R2220 T3288 T3290 ccomp presented,denotes
R2221 T3289 T3288 auxpass are,presented
R2222 T3291 T3288 prep as,presented
R2223 T3292 T3293 compound mean,sem
R2224 T3293 T3291 pobj sem,as
R2225 T3294 T3293 compound ±,sem
R2226 T3295 T3290 punct ;,denotes
R2227 T3296 T3290 nsubj n,denotes
R2228 T3297 T3298 det the,number
R2229 T3298 T3290 dobj number,denotes
R2230 T3299 T3298 prep of,number
R2231 T3300 T3299 pobj experiments,of
R2232 T3301 T3290 punct .,denotes
R2233 T3303 T3304 nsubjpass Differences,considered
R2234 T3305 T3304 auxpass were,considered
R2235 T3306 T3304 oprd significant,considered
R2236 T3307 T3308 advmod when,0.05
R2237 T3308 T3304 advcl 0.05,considered
R2238 T3309 T3308 nsubj p,0.05
R2239 T3310 T3308 punct <,0.05
R2240 T3311 T3304 punct .,considered
R1672 T2615 T2612 pobj electrophoresis,by

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2832 6616-6618 CHEBI_EXT:platinum denotes Pt
T2831 6445-6455 UBERON:0000483 denotes epithelium
T2830 6377-6379 CHEBI_EXT:platinum denotes Pt
T2829 6347-6349 CHEBI_EXT:iridium denotes Ir
T2828 6344-6346 CHEBI_EXT:platinum denotes Pt
T2827 6311-6321 UBERON:0000483 denotes epithelium
T2826 6296-6301 UBERON:0002282 denotes stria
T2825 6221-6236 UBERON:0006725 denotes spiral ligament
T2824 6196-6212 UBERON:0002282 denotes Stria vascularis
T2823 6157-6177 CHEBI:7984 denotes sodium pentobarbital
T2822 6139-6151 CHEBI_EXT:anaesthetic_process denotes anesthetized
T2821 6119-6126 NCBITaxon:33208 denotes Animals
T3049 7861-7866 UBERON:0000178 denotes blood
T3048 7821-7823 CHEBI:29103 denotes K+
T3047 7814-7820 UBERON:0001969 denotes Plasma
T3046 7805-7812 UBERON:0001844 denotes cochlea
T3045 7707-7710 CHEBI:29101 denotes Na+
T3044 7699-7702 CHEBI:29101 denotes Na+
T3043 7629-7632 CHEBI:29101 denotes Na+
T3042 7618-7620 CHEBI:29103 denotes K+
T3041 7426-7429 CHEBI:29101 denotes Na+
T3040 7408-7410 CHEBI:29103 denotes K+
T3039 7349-7352 CHEBI:29101 denotes Na+
T3038 7342-7344 CHEBI:29103 denotes K+
T3037 7334-7340 CHEBI:36916 denotes cation
T3036 7312-7321 CHEBI:75958 denotes solutions
T3035 7269-7271 CHEBI:29103 denotes K+
T3034 7217-7233 UBERON:0002282 denotes stria vascularis
T3033 7203-7207 UBERON_EXT:bone_element_or_tissue denotes bone
T3032 7144-7160 UBERON:0002226 denotes basilar membrane
T3031 7003-7005 CHEBI:29103 denotes K+
T3030 6988-7001 UBERON:0001852 denotes endolymphatic
T3029 6925-6936 CHEBI:26714 denotes sodium salt
T3028 6881-6893 CHEBI_EXT:anaesthetic_process denotes anesthetized
T3027 6868-6875 NCBITaxon:33208 denotes Animals
T2209 6082-6084 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T2208 6019-6028 UBERON:0001846 denotes inner ear
T2207 5994-6001 SO_EXT:0001026 denotes Genomic
T2206 5978-5984 UBERON:0000479 denotes tissue
T2205 5952-5956 CHEBI_SO_EXT:rRNA denotes rRNA
T2204 5948-5956 SO_EXT:0000407 denotes 18S rRNA
T2203 5894-5898 CHEBI_SO_EXT:rRNA denotes rRNA
T2202 5890-5898 SO_EXT:0000407 denotes 18S rRNA
T2201 5809-5818 CHEBI:36357 denotes molecules
T2200 5532-5541 CHEBI:36357 denotes molecules
T2199 5436-5445 CHEBI:36357 denotes molecules
T2198 5344-5353 CHEBI:36357 denotes molecules
T1269 61-74 UBERON:0001845 denotes perilymphatic
T1270 75-77 CHEBI:29103 denotes K+
T1271 116-121 UBERON:0007023 denotes adult
T1272 122-126 NCBITaxon:10088 denotes mice
T1273 164-171 PR_EXT:000015039 denotes Slc26a4
T1274 171-172 SO_EXT:normal_or_wild_type_or_present denotes +
T1275 173-174 SO_EXT:normal_or_wild_type_or_present denotes +
T1276 185-192 PR_EXT:000015039 denotes Slc26a4
T1277 192-193 SO_EXT:sequence_nullness_or_absence denotes -
T1278 194-195 SO_EXT:sequence_nullness_or_absence denotes -
T1279 210-214 SO_EXT:0000704 denotes gene
T1280 219-226 PR_EXT:000015039 denotes pendrin
T1281 237-242 NCBITaxon:10088 denotes mouse
T1282 313-320 PR_EXT:000015039 denotes Slc26a4
T1283 320-321 SO_EXT:normal_or_wild_type_or_present denotes +
T1284 322-323 SO_EXT:normal_or_wild_type_or_present denotes +
T1285 340-347 PR_EXT:000015039 denotes Slc26a4
T1286 347-348 SO_EXT:sequence_nullness_or_absence denotes -
T1287 349-350 SO_EXT:sequence_nullness_or_absence denotes -
T1288 351-355 NCBITaxon:10088 denotes mice
T1289 413-422 CL:0000034 denotes stem cell
T1290 418-422 CL_GO_EXT:cell denotes cell
T1291 451-458 PR_EXT:000015039 denotes Slc26a4
T1292 458-459 SO_EXT:normal_or_wild_type_or_present denotes +
T1293 460-461 SO_EXT:normal_or_wild_type_or_present denotes +
T1294 466-473 PR_EXT:000015039 denotes Slc26a4
T1295 473-474 SO_EXT:sequence_nullness_or_absence denotes -
T1296 475-476 SO_EXT:sequence_nullness_or_absence denotes -
T1297 513-517 UBERON:0010166 denotes coat
T1298 540-552 GO_PATO_EXT:biological_pigmentation_process_or_quality denotes pigmentation
T1299 596-603 PR_EXT:000015039 denotes Slc26a4
T1300 603-604 SO_EXT:normal_or_wild_type_or_present denotes +
T1301 605-606 SO_EXT:normal_or_wild_type_or_present denotes +
T1302 611-618 PR_EXT:000015039 denotes Slc26a4
T1303 618-619 SO_EXT:sequence_nullness_or_absence denotes -
T1304 620-621 SO_EXT:sequence_nullness_or_absence denotes -
T1305 632-636 GO_EXT:breeding denotes bred
T1306 650-660 GO:0010467 denotes Expression
T1307 668-676 CHEBI_PR_EXT:protein denotes proteins
T1308 742-757 GO:0006813 denotes transport of K+
T1309 755-757 CHEBI:29103 denotes K+
T1310 822-824 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T1311 830-832 CHEBI:29103 denotes K+
T1312 833-842 GO_EXT:secretion_entity_or_process denotes secretion
T1313 996-1002 NCBITaxon:33208 denotes Animal
T1618 1084-1091 NCBITaxon:33208 denotes Animals
T1619 1104-1116 CHEBI_EXT:anaesthetic_process denotes anesthetized
T1620 1122-1142 CHEBI:7984 denotes sodium pentobarbital
T1621 1169-1178 UBERON:0000948 denotes cardially
T1622 1195-1198 CHEBI:17996 denotes Cl-
T1623 1204-1213 CHEBI_EXT:phosphate denotes phosphate
T1624 1214-1222 CHEBI_CHMO_EXT:buffer_process denotes buffered
T1625 1223-1235 CHEBI:84997 denotes Na-gluconate
T1626 1236-1244 CHEBI:75958 denotes solution
T1627 1259-1275 CHEBI_EXT:paraformaldehye denotes paraformaldehyde
T1628 1277-1291 UBERON:0001678 denotes Temporal bones
T1629 1309-1317 UBERON:0001844 denotes cochleae
T1630 1327-1340 UBERON:0001845 denotes perilymphatic
T1631 1367-1371 CHEBI_EXT:EDTA denotes EDTA
T1632 1393-1400 CHEBI:17992 denotes sucrose
T1633 1431-1450 CHEBI:46793 denotes polyethylene glycol
T1634 1456-1464 UBERON:0006723 denotes modiolar
T1635 1551-1559 CHEBI:9750 denotes Triton-X
T1636 1565-1567 CHEBI:9750 denotes TX
T1637 1577-1583 NCBITaxon:27592 denotes bovine
T1638 1584-1589 UBERON:0001977 denotes serum
T1639 1584-1597 PR_EXT:000003918 denotes serum albumin
T1640 1651-1661 GO:0042571 denotes antibodies
T1641 1669-1671 CHEBI:9750 denotes TX
T1642 1687-1693 NCBITaxon:9986 denotes rabbit
T1643 1699-1706 PR_EXT:000015039 denotes pendrin
T1644 1726-1729 NCBITaxon:10114 denotes rat
T1645 1735-1739 PR_EXT:000016364 denotes ZO-1
T1646 1773-1777 NCBITaxon:9925 denotes goat
T1647 1783-1788 PR_EXT:000000728 denotes KCNQ1
T1648 1845-1851 NCBITaxon:9986 denotes rabbit
T1649 1857-1862 PR_EXT:000009255 denotes KCNE1
T1650 1900-1906 NCBITaxon:9986 denotes rabbit
T1651 1912-1918 PR_EXT:000001979 denotes KCNJ10
T1652 1937-1943 NCBITaxon:9986 denotes rabbit
T1653 1949-1956 PR_EXT:000014925 denotes SLC12A2
T1654 1980-1986 NCBITaxon:9986 denotes rabbit
T1655 1992-2003 PR_EXT:000007998 denotes connexin 26
T1656 2066-2068 CHEBI:9750 denotes TX
T1657 2126-2136 GO:0042571 denotes antibodies
T1658 2165-2167 CHEBI:9750 denotes TX
T1659 2183-2189 NCBITaxon:9793 denotes donkey
T1660 2195-2201 NCBITaxon:9986 denotes rabbit
T1661 2202-2211 CHEBI:52661 denotes Alexa 488
T1662 2213-2220 NCBITaxon:9031 denotes chicken
T1663 2226-2229 NCBITaxon:10114 denotes rat
T1664 2230-2239 CHEBI:51248 denotes Alexa 594
T1665 2245-2252 NCBITaxon:9031 denotes chicken
T1666 2258-2262 NCBITaxon:9925 denotes goat
T1667 2263-2272 CHEBI:51248 denotes Alexa 594
T1668 2274-2283 CHEBI_EXT:polyatomic_entity_or_group denotes Molecular
T1669 2284-2290 CHEBI_SO_EXT:molecular_probe denotes Probes
T1670 2306-2321 GO:0005884 denotes Actin filaments
T1671 2355-2364 CHEBI:52661 denotes Alexa 488
T1672 2365-2375 MOP:0000779 denotes conjugated
T1673 2376-2386 CHEBI:8040 denotes phalloidin
T1674 2388-2397 CHEBI_EXT:polyatomic_entity_or_group denotes Molecular
T1675 2398-2404 CHEBI_SO_EXT:molecular_probe denotes Probes
T1676 2453-2455 CHEBI:9750 denotes TX
T1677 2746-2758 GO_PATO_EXT:biological_pigmentation_process_or_quality denotes pigmentation
T2140 2774-2776 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T2141 2781-2788 NCBITaxon:33208 denotes Animals
T2142 2801-2813 CHEBI_EXT:anaesthetic_process denotes anesthetized
T2143 2819-2839 CHEBI:7984 denotes sodium pentobarbital
T2144 2858-2865 UBERON:0002113 denotes Kidneys
T2145 2870-2875 UBERON:0000955 denotes brain
T2146 2914-2916 CHEBI:17997 denotes N2
T2147 2918-2932 UBERON:0001678 denotes Temporal bones
T2148 2970-2979 UBERON:0001846 denotes inner ear
T2149 2980-2987 UBERON:0000479 denotes tissues
T2150 3026-3042 UBERON:0002282 denotes stria vascularis
T2151 3051-3066 UBERON:0006725 denotes spiral ligament
T2152 3076-3090 UBERON:0000395 denotes spiral ganglia
T2153 3117-3121 UBERON_EXT:bone_element_or_tissue denotes bone
T2154 3130-3144 UBERON:0002227 denotes organ of Corti
T2155 3226-3233 UBERON:0000479 denotes tissues
T2156 3295-3302 PR_EXT:000015039 denotes Slc26a4
T2157 3302-3303 SO_EXT:sequence_nullness_or_absence denotes -
T2158 3304-3305 SO_EXT:sequence_nullness_or_absence denotes -
T2159 3305-3313 SO_EXT:genotype_or_entity_with_genotype denotes genotype
T2160 3355-3363 UBERON:0002280 denotes otoconia
T2161 3371-3387 UBERON:0002214 denotes utricular macula
T2162 3400-3403 CHEBI_SO_EXT:RNA denotes RNA
T2163 3430-3433 CHEBI_SO_EXT:DNA denotes DNA
T2164 3463-3472 CHEBI_GO_SO_EXT:enzyme denotes enzymatic
T2165 3545-3553 SO_EXT:0000407 denotes 18S rRNA
T2167 3568-3575 UBERON:0002113 denotes kidneys
T2168 3580-3585 UBERON:0000955 denotes brain
T2169 3682-3685 CHEBI_SO_EXT:RNA denotes RNA
T2170 3713-3729 UBERON:0002282 denotes stria vascularis
T2171 3734-3748 UBERON:0000395 denotes spiral ganglia
T2172 3804-3806 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T2173 3828-3831 CHEBI_SO_EXT:RNA denotes RNA
T2174 3857-3864 NCBITaxon:33208 denotes animals
T2175 3874-3876 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T2176 3952-3964 CHEBI:51461 denotes SYBR green I
T2177 3966-3975 CHEBI_EXT:polyatomic_entity_or_group denotes Molecular
T2178 3976-3982 CHEBI_SO_EXT:molecular_probe denotes Probes
T2179 3985-3996 SO_EXT:0000673 denotes Transcripts
T2180 4000-4008 SO_EXT:0000407 denotes 18S rRNA
T2181 4004-4008 CHEBI_SO_EXT:rRNA denotes rRNA
T2182 4013-4017 CHEBI_SO_EXT:mRNA denotes mRNA
T2183 4026-4028 CHEBI:29103 denotes K+
T2184 4026-4037 GO_EXT:0005267 denotes K+ channels
T2185 4038-4044 PR_EXT:000001979 denotes KCNJ10
T2186 4046-4051 PR_EXT:000000728 denotes KCNQ1
T2187 4056-4061 PR_EXT:000000731 denotes KCNQ4
T2188 4083-4087 SO_EXT:0000704 denotes gene
T2189 4097-4104 SO_EXT:0000112 denotes primers
T2190 4116-4118 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T2191 4318-4325 GO:0032508 denotes melting
T2192 4429-4435 SO_EXT:0000112 denotes primer
T2193 4436-4442 GO_EXT:dimer_macromolecular_complex denotes dimers
T2194 4452-4459 GO:0032508 denotes melting
T2195 4521-4525 GO:0032508 denotes melt
T2196 4645-4649 GO:0032508 denotes melt
T2197 4662-4669 CHEBI:2511 denotes agarose
T7758 4746-4753 SO_EXT:0000112 denotes Primers
T2166 3549-3553 CHEBI_SO_EXT:rRNA denotes rRNA

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T2820 6445-6455 UBERON:0000483 denotes epithelium
T2819 6311-6321 UBERON:0000483 denotes epithelium
T2818 6296-6301 UBERON:0002282 denotes stria
T2817 6221-6236 UBERON:0006725 denotes spiral ligament
T2816 6196-6212 UBERON:0002282 denotes Stria vascularis
T2815 6157-6177 CHEBI:7984 denotes sodium pentobarbital
T2814 6119-6126 NCBITaxon:33208 denotes Animals
T3026 7861-7866 UBERON:0000178 denotes blood
T3025 7821-7823 CHEBI:29103 denotes K+
T3024 7814-7820 UBERON:0001969 denotes Plasma
T3023 7805-7812 UBERON:0001844 denotes cochlea
T3022 7707-7710 CHEBI:29101 denotes Na+
T3021 7699-7702 CHEBI:29101 denotes Na+
T3020 7629-7632 CHEBI:29101 denotes Na+
T3019 7618-7620 CHEBI:29103 denotes K+
T3018 7426-7429 CHEBI:29101 denotes Na+
T3017 7408-7410 CHEBI:29103 denotes K+
T3016 7349-7352 CHEBI:29101 denotes Na+
T3015 7342-7344 CHEBI:29103 denotes K+
T3014 7334-7340 CHEBI:36916 denotes cation
T3013 7312-7321 CHEBI:75958 denotes solutions
T3012 7269-7271 CHEBI:29103 denotes K+
T3011 7217-7233 UBERON:0002282 denotes stria vascularis
T3010 7144-7160 UBERON:0002226 denotes basilar membrane
T3009 7003-7005 CHEBI:29103 denotes K+
T3008 6988-7001 UBERON:0001852 denotes endolymphatic
T3007 6925-6936 CHEBI:26714 denotes sodium salt
T3006 6868-6875 NCBITaxon:33208 denotes Animals
T2139 6019-6028 UBERON:0001846 denotes inner ear
T2138 5994-6001 SO:0001026 denotes Genomic
T2137 5978-5984 UBERON:0000479 denotes tissue
T2136 5948-5956 SO:0000407 denotes 18S rRNA
T2135 5890-5898 SO:0000407 denotes 18S rRNA
T2134 5809-5818 CHEBI:36357 denotes molecules
T2133 5532-5541 CHEBI:36357 denotes molecules
T2132 5436-5445 CHEBI:36357 denotes molecules
T2131 5344-5353 CHEBI:36357 denotes molecules
T1245 61-74 UBERON:0001845 denotes perilymphatic
T1246 75-77 CHEBI:29103 denotes K+
T1247 116-121 UBERON:0007023 denotes adult
T1248 122-126 NCBITaxon:10088 denotes mice
T1249 164-171 PR:000015039 denotes Slc26a4
T1250 185-192 PR:000015039 denotes Slc26a4
T1251 210-214 SO:0000704 denotes gene
T1252 219-226 PR:000015039 denotes pendrin
T1253 237-242 NCBITaxon:10088 denotes mouse
T1254 313-320 PR:000015039 denotes Slc26a4
T1255 340-347 PR:000015039 denotes Slc26a4
T1256 351-355 NCBITaxon:10088 denotes mice
T1257 413-422 CL:0000034 denotes stem cell
T1258 451-458 PR:000015039 denotes Slc26a4
T1259 466-473 PR:000015039 denotes Slc26a4
T1260 513-517 UBERON:0010166 denotes coat
T1261 596-603 PR:000015039 denotes Slc26a4
T1262 611-618 PR:000015039 denotes Slc26a4
T1263 650-660 GO:0010467 denotes Expression
T1264 742-757 GO:0006813 denotes transport of K+
T1265 755-757 CHEBI:29103 denotes K+
T1266 830-832 CHEBI:29103 denotes K+
T1267 996-1002 NCBITaxon:33208 denotes Animal
T1568 1084-1091 NCBITaxon:33208 denotes Animals
T1569 1122-1142 CHEBI:7984 denotes sodium pentobarbital
T1570 1169-1178 UBERON:0000948 denotes cardially
T1571 1195-1198 CHEBI:17996 denotes Cl-
T1572 1223-1235 CHEBI:84997 denotes Na-gluconate
T1573 1236-1244 CHEBI:75958 denotes solution
T1574 1277-1291 UBERON:0001678 denotes Temporal bones
T1575 1309-1317 UBERON:0001844 denotes cochleae
T1576 1327-1340 UBERON:0001845 denotes perilymphatic
T1577 1393-1400 CHEBI:17992 denotes sucrose
T1578 1431-1450 CHEBI:46793 denotes polyethylene glycol
T1579 1456-1464 UBERON:0006723 denotes modiolar
T1580 1551-1559 CHEBI:9750 denotes Triton-X
T1581 1565-1567 CHEBI:9750 denotes TX
T1582 1577-1583 NCBITaxon:27592 denotes bovine
T1583 1584-1589 UBERON:0001977 denotes serum
T1584 1584-1597 PR:000003918 denotes serum albumin
T1585 1651-1661 GO:0042571 denotes antibodies
T1586 1669-1671 CHEBI:9750 denotes TX
T1587 1687-1693 NCBITaxon:9986 denotes rabbit
T1588 1699-1706 PR:000015039 denotes pendrin
T1589 1726-1729 NCBITaxon:10114 denotes rat
T1590 1735-1739 PR:000016364 denotes ZO-1
T1591 1773-1777 NCBITaxon:9925 denotes goat
T1592 1783-1788 PR:000000728 denotes KCNQ1
T1593 1845-1851 NCBITaxon:9986 denotes rabbit
T1594 1857-1862 PR:000009255 denotes KCNE1
T1595 1900-1906 NCBITaxon:9986 denotes rabbit
T1596 1912-1918 PR:000001979 denotes KCNJ10
T1597 1937-1943 NCBITaxon:9986 denotes rabbit
T1598 1949-1956 PR:000014925 denotes SLC12A2
T1599 1980-1986 NCBITaxon:9986 denotes rabbit
T1600 1992-2003 PR:000007998 denotes connexin 26
T1601 2066-2068 CHEBI:9750 denotes TX
T1602 2126-2136 GO:0042571 denotes antibodies
T1603 2165-2167 CHEBI:9750 denotes TX
T1604 2183-2189 NCBITaxon:9793 denotes donkey
T1605 2195-2201 NCBITaxon:9986 denotes rabbit
T1606 2202-2211 CHEBI:52661 denotes Alexa 488
T1607 2213-2220 NCBITaxon:9031 denotes chicken
T1608 2226-2229 NCBITaxon:10114 denotes rat
T1609 2230-2239 CHEBI:51248 denotes Alexa 594
T1610 2245-2252 NCBITaxon:9031 denotes chicken
T1611 2258-2262 NCBITaxon:9925 denotes goat
T1612 2263-2272 CHEBI:51248 denotes Alexa 594
T1613 2306-2321 GO:0005884 denotes Actin filaments
T1614 2355-2364 CHEBI:52661 denotes Alexa 488
T1615 2365-2375 MOP:0000779 denotes conjugated
T1616 2376-2386 CHEBI:8040 denotes phalloidin
T1617 2453-2455 CHEBI:9750 denotes TX
T2094 2781-2788 NCBITaxon:33208 denotes Animals
T2095 2819-2839 CHEBI:7984 denotes sodium pentobarbital
T2096 2858-2865 UBERON:0002113 denotes Kidneys
T2097 2870-2875 UBERON:0000955 denotes brain
T2098 2914-2916 CHEBI:17997 denotes N2
T2099 2918-2932 UBERON:0001678 denotes Temporal bones
T2100 2970-2979 UBERON:0001846 denotes inner ear
T2101 2980-2987 UBERON:0000479 denotes tissues
T2102 3026-3042 UBERON:0002282 denotes stria vascularis
T2103 3051-3066 UBERON:0006725 denotes spiral ligament
T2104 3076-3090 UBERON:0000395 denotes spiral ganglia
T2105 3130-3144 UBERON:0002227 denotes organ of Corti
T2106 3226-3233 UBERON:0000479 denotes tissues
T2107 3295-3302 PR:000015039 denotes Slc26a4
T2108 3355-3363 UBERON:0002280 denotes otoconia
T2109 3371-3387 UBERON:0002214 denotes utricular macula
T2110 3545-3553 SO:0000407 denotes 18S rRNA
T2111 3568-3575 UBERON:0002113 denotes kidneys
T2112 3580-3585 UBERON:0000955 denotes brain
T2113 3713-3729 UBERON:0002282 denotes stria vascularis
T2114 3734-3748 UBERON:0000395 denotes spiral ganglia
T2115 3857-3864 NCBITaxon:33208 denotes animals
T2116 3952-3964 CHEBI:51461 denotes SYBR green I
T2117 3985-3996 SO:0000673 denotes Transcripts
T2118 4000-4008 SO:0000407 denotes 18S rRNA
T2119 4026-4028 CHEBI:29103 denotes K+
T2120 4038-4044 PR:000001979 denotes KCNJ10
T2121 4046-4051 PR:000000728 denotes KCNQ1
T2122 4056-4061 PR:000000731 denotes KCNQ4
T2123 4083-4087 SO:0000704 denotes gene
T2124 4097-4104 SO:0000112 denotes primers
T2125 4318-4325 GO:0032508 denotes melting
T2126 4429-4435 SO:0000112 denotes primer
T2127 4452-4459 GO:0032508 denotes melting
T2128 4521-4525 GO:0032508 denotes melt
T2129 4645-4649 GO:0032508 denotes melt
T2130 4662-4669 CHEBI:2511 denotes agarose
T7757 4746-4753 SO:0000112 denotes Primers

2_test

Id Subject Object Predicate Lexical cue
15320950-11152663-12070953 228-230 11152663 denotes 14
15320950-11152663-12070954 3389-3391 11152663 denotes 14
15320950-10079033-12070955 4494-4496 10079033 denotes 15
15320950-10079033-12070956 5570-5572 10079033 denotes 15
15320950-11717350-12070957 6337-6339 11717350 denotes 16
15320950-11788352-12070958 7059-7061 11788352 denotes 17