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endocochlear potential is generated by stria vascularis in the lateral wall of the cochlea [17,23]. The potential is generated across the basal cell barrier of stria vascularis by the K+ channel KCNJ10 located in intermediate cells [24], which are connected to basal cells by a high density of gap junctions [25]. Marginal cells of stria vascularis, which form the barrier toward endolymph, transport K+ from the intrastrial space into endolymph and keep the K+ concentration low adjacent to the KCNJ10 K+ channel [26]. To determine the cause of the loss of the endocochlear potential in Slc26a4-/- mice, we first determined whether intermediate cells are present in stria vascularis, since a loss of intermediate cells is known to lead to a loss of the endocochlear potential [27,28]. Intermediate cells of stria vascularis were visualized by their pigmentation. Pigmentation was present in stria vascularis of Slc26a4-/- mice (Fig. 2b), which suggests that intermediate cells are present. Interestingly, pigmentation of stria vascularis was much stronger in Slc26a4-/- than in Slc26a4+/+ mice."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T4373","span":{"begin":1094,"end":1098},"obj":"NCBITaxon:10088"},{"id":"T4372","span":{"begin":1092,"end":1093},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T4371","span":{"begin":1090,"end":1091},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T4370","span":{"begin":1083,"end":1090},"obj":"PR_EXT:000015039"},{"id":"T4369","span":{"begin":1073,"end":1074},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4368","span":{"begin":1071,"end":1072},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4367","span":{"begin":1064,"end":1071},"obj":"PR_EXT:000015039"},{"id":"T4366","span":{"begin":1026,"end":1042},"obj":"UBERON:0002282"},{"id":"T4365","span":{"begin":1010,"end":1022},"obj":"GO_PATO_EXT:biological_pigmentation_process_or_quality"},{"id":"T4364","span":{"begin":976,"end":981},"obj":"CL_GO_EXT:cell"},{"id":"T4363","span":{"begin":963,"end":981},"obj":"CL:0002486"},{"id":"T4362","span":{"begin":927,"end":931},"obj":"NCBITaxon:10088"},{"id":"T4361","span":{"begin":925,"end":926},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4360","span":{"begin":923,"end":924},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4359","span":{"begin":916,"end":923},"obj":"PR_EXT:000015039"},{"id":"T4358","span":{"begin":896,"end":912},"obj":"UBERON:0002282"},{"id":"T4357","span":{"begin":868,"end":880},"obj":"GO_PATO_EXT:biological_pigmentation_process_or_quality"},{"id":"T4356","span":{"begin":854,"end":866},"obj":"GO_PATO_EXT:biological_pigmentation_process_or_quality"},{"id":"T4355","span":{"begin":812,"end":828},"obj":"UBERON:0002282"},{"id":"T4354","span":{"begin":803,"end":808},"obj":"CL_GO_EXT:cell"},{"id":"T4353","span":{"begin":790,"end":808},"obj":"CL:0002486"},{"id":"T4352","span":{"begin":718,"end":723},"obj":"CL_GO_EXT:cell"},{"id":"T4351","span":{"begin":705,"end":723},"obj":"CL:0002486"},{"id":"T4350","span":{"begin":671,"end":687},"obj":"UBERON:0002282"},{"id":"T4349","span":{"begin":650,"end":655},"obj":"CL_GO_EXT:cell"},{"id":"T4348","span":{"begin":637,"end":655},"obj":"CL:0002486"},{"id":"T4347","span":{"begin":603,"end":607},"obj":"NCBITaxon:10088"},{"id":"T4346","span":{"begin":601,"end":602},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4345","span":{"begin":599,"end":600},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T4344","span":{"begin":592,"end":599},"obj":"PR_EXT:000015039"},{"id":"T4343","span":{"begin":507,"end":517},"obj":"GO_EXT:0005267"},{"id":"T4342","span":{"begin":507,"end":509},"obj":"CHEBI:29103"},{"id":"T4341","span":{"begin":500,"end":506},"obj":"PR_EXT:000001979"},{"id":"T4340","span":{"begin":463,"end":465},"obj":"CHEBI:29103"},{"id":"T4339","span":{"begin":440,"end":449},"obj":"UBERON:0001852"},{"id":"T4338","span":{"begin":422,"end":428},"obj":"UBERON:0002282"},{"id":"T4337","span":{"begin":405,"end":407},"obj":"CHEBI:29103"},{"id":"T4336","span":{"begin":395,"end":404},"obj":"GO_EXT:biological_routing_or_transport"},{"id":"T4335","span":{"begin":384,"end":393},"obj":"UBERON:0001852"},{"id":"T4334","span":{"begin":336,"end":352},"obj":"UBERON:0002282"},{"id":"T4333","span":{"begin":327,"end":332},"obj":"CL_GO_EXT:cell"},{"id":"T4332","span":{"begin":318,"end":332},"obj":"CL:0002492"},{"id":"T4331","span":{"begin":298,"end":311},"obj":"GO:0005921"},{"id":"T4330","span":{"begin":271,"end":276},"obj":"CL_GO_EXT:cell"},{"id":"T4329","span":{"begin":265,"end":276},"obj":"CL:0000646"},{"id":"T4328","span":{"begin":230,"end":235},"obj":"CL_GO_EXT:cell"},{"id":"T4327","span":{"begin":217,"end":235},"obj":"CL:0002486"},{"id":"T4326","span":{"begin":199,"end":205},"obj":"PR_EXT:000001979"},{"id":"T4325","span":{"begin":188,"end":198},"obj":"GO_EXT:0005267"},{"id":"T4324","span":{"begin":188,"end":190},"obj":"CHEBI:29103"},{"id":"T4323","span":{"begin":164,"end":180},"obj":"UBERON:0002282"},{"id":"T4322","span":{"begin":148,"end":152},"obj":"CL_GO_EXT:cell"},{"id":"T4321","span":{"begin":142,"end":152},"obj":"CL:0000646"},{"id":"T4320","span":{"begin":87,"end":94},"obj":"UBERON:0006106"},{"id":"T4319","span":{"begin":75,"end":82},"obj":"_FRAGMENT"},{"id":"T4318","span":{"begin":43,"end":59},"obj":"UBERON:0002282"}],"relations":[{"id":"R2259","pred":"_lexicallyChainedTo","subj":"T4320","obj":"T4319"}],"text":"The endocochlear potential is generated by stria vascularis in the lateral wall of the cochlea [17,23]. The potential is generated across the basal cell barrier of stria vascularis by the K+ channel KCNJ10 located in intermediate cells [24], which are connected to basal cells by a high density of gap junctions [25]. Marginal cells of stria vascularis, which form the barrier toward endolymph, transport K+ from the intrastrial space into endolymph and keep the K+ concentration low adjacent to the KCNJ10 K+ channel [26]. To determine the cause of the loss of the endocochlear potential in Slc26a4-/- mice, we first determined whether intermediate cells are present in stria vascularis, since a loss of intermediate cells is known to lead to a loss of the endocochlear potential [27,28]. Intermediate cells of stria vascularis were visualized by their pigmentation. Pigmentation was present in stria vascularis of Slc26a4-/- mice (Fig. 2b), which suggests that intermediate cells are present. Interestingly, pigmentation of stria vascularis was much stronger in Slc26a4-/- than in Slc26a4+/+ mice."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T3885","span":{"begin":1094,"end":1098},"obj":"NCBITaxon:10088"},{"id":"T3884","span":{"begin":1083,"end":1090},"obj":"PR:000015039"},{"id":"T3883","span":{"begin":1064,"end":1071},"obj":"PR:000015039"},{"id":"T3882","span":{"begin":1026,"end":1042},"obj":"UBERON:0002282"},{"id":"T3881","span":{"begin":963,"end":981},"obj":"CL:0002486"},{"id":"T3880","span":{"begin":927,"end":931},"obj":"NCBITaxon:10088"},{"id":"T3879","span":{"begin":916,"end":923},"obj":"PR:000015039"},{"id":"T3878","span":{"begin":896,"end":912},"obj":"UBERON:0002282"},{"id":"T3877","span":{"begin":812,"end":828},"obj":"UBERON:0002282"},{"id":"T3876","span":{"begin":790,"end":808},"obj":"CL:0002486"},{"id":"T3875","span":{"begin":705,"end":723},"obj":"CL:0002486"},{"id":"T3874","span":{"begin":671,"end":687},"obj":"UBERON:0002282"},{"id":"T3873","span":{"begin":637,"end":655},"obj":"CL:0002486"},{"id":"T3872","span":{"begin":603,"end":607},"obj":"NCBITaxon:10088"},{"id":"T3871","span":{"begin":592,"end":599},"obj":"PR:000015039"},{"id":"T3870","span":{"begin":507,"end":509},"obj":"CHEBI:29103"},{"id":"T3869","span":{"begin":500,"end":506},"obj":"PR:000001979"},{"id":"T3868","span":{"begin":463,"end":465},"obj":"CHEBI:29103"},{"id":"T3867","span":{"begin":440,"end":449},"obj":"UBERON:0001852"},{"id":"T3866","span":{"begin":422,"end":428},"obj":"UBERON:0002282"},{"id":"T3865","span":{"begin":405,"end":407},"obj":"CHEBI:29103"},{"id":"T3864","span":{"begin":384,"end":393},"obj":"UBERON:0001852"},{"id":"T3863","span":{"begin":336,"end":352},"obj":"UBERON:0002282"},{"id":"T3862","span":{"begin":318,"end":332},"obj":"CL:0002492"},{"id":"T3861","span":{"begin":298,"end":311},"obj":"GO:0005921"},{"id":"T3860","span":{"begin":265,"end":276},"obj":"CL:0000646"},{"id":"T3859","span":{"begin":217,"end":235},"obj":"CL:0002486"},{"id":"T3858","span":{"begin":199,"end":205},"obj":"PR:000001979"},{"id":"T3856","span":{"begin":164,"end":180},"obj":"UBERON:0002282"},{"id":"T3857","span":{"begin":188,"end":190},"obj":"CHEBI:29103"},{"id":"T3855","span":{"begin":142,"end":152},"obj":"CL:0000646"},{"id":"T3854","span":{"begin":87,"end":94},"obj":"UBERON:0006106"},{"id":"T3853","span":{"begin":75,"end":82},"obj":"_FRAGMENT"},{"id":"T3852","span":{"begin":43,"end":59},"obj":"UBERON:0002282"}],"relations":[{"id":"R2245","pred":"_lexicallyChainedTo","subj":"T3854","obj":"T3853"}],"text":"The endocochlear potential is generated by stria vascularis in the lateral wall of the cochlea [17,23]. The potential is generated across the basal cell barrier of stria vascularis by the K+ channel KCNJ10 located in intermediate cells [24], which are connected to basal cells by a high density of gap junctions [25]. Marginal cells of stria vascularis, which form the barrier toward endolymph, transport K+ from the intrastrial space into endolymph and keep the K+ concentration low adjacent to the KCNJ10 K+ channel [26]. To determine the cause of the loss of the endocochlear potential in Slc26a4-/- mice, we first determined whether intermediate cells are present in stria vascularis, since a loss of intermediate cells is known to lead to a loss of the endocochlear potential [27,28]. Intermediate cells of stria vascularis were visualized by their pigmentation. Pigmentation was present in stria vascularis of Slc26a4-/- mice (Fig. 2b), which suggests that intermediate cells are present. Interestingly, pigmentation of stria vascularis was much stronger in Slc26a4-/- than in Slc26a4+/+ mice."}

{"project":"2_test","denotations":[{"id":"15320950-11788352-12070971","span":{"begin":96,"end":98},"obj":"11788352"},{"id":"15320950-12031509-12070972","span":{"begin":99,"end":101},"obj":"12031509"},{"id":"15320950-10409112-12070973","span":{"begin":237,"end":239},"obj":"10409112"},{"id":"15320950-9662649-12070974","span":{"begin":313,"end":315},"obj":"9662649"},{"id":"15320950-7642451-12070975","span":{"begin":519,"end":521},"obj":"7642451"},{"id":"15320950-2380119-12070976","span":{"begin":782,"end":784},"obj":"2380119"},{"id":"15320950-2076537-12070977","span":{"begin":785,"end":787},"obj":"2076537"}],"text":"The endocochlear potential is generated by stria vascularis in the lateral wall of the cochlea [17,23]. The potential is generated across the basal cell barrier of stria vascularis by the K+ channel KCNJ10 located in intermediate cells [24], which are connected to basal cells by a high density of gap junctions [25]. Marginal cells of stria vascularis, which form the barrier toward endolymph, transport K+ from the intrastrial space into endolymph and keep the K+ concentration low adjacent to the KCNJ10 K+ channel [26]. To determine the cause of the loss of the endocochlear potential in Slc26a4-/- mice, we first determined whether intermediate cells are present in stria vascularis, since a loss of intermediate cells is known to lead to a loss of the endocochlear potential [27,28]. Intermediate cells of stria vascularis were visualized by their pigmentation. Pigmentation was present in stria vascularis of Slc26a4-/- mice (Fig. 2b), which suggests that intermediate cells are present. Interestingly, pigmentation of stria vascularis was much stronger in Slc26a4-/- than in Slc26a4+/+ mice."}