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situ electrophysiology\nAnimals were anesthetized with inactin (thiobutabarbital sodium salt, 140 mg/kg ip). The endocochlear potential and the endolymphatic [K+] were measured with double-barreled microelectrodes [17]. Measurements were made in the basal turn by a round-window approach through the basilar membrane and in the apical turn after thinning the bone over the stria vascularis and picking a small hole (~30 μm). K+-selective electrodes were calibrated in solutions of constant cation (K+ and Na+) concentration of 150 mM. The minor selectivity of the K+ electrodes for Na+ produced a nonlinearity in the calibration curve, which was closely fitted by the Nicolski equation using nonlinear curve-fitting software (OriginLab, Northampton, MA): V = Vi + S × log ([K+] + A × [Na+]), where Vi is an offset term, S is slope, A is selectivity, and [Na+] is Na+ concentration. Calibrations were made immediately after withdrawal of the electrodes from the cochlea. Plasma K+ concentrations were obtained using a blood analyzer (Stat Profile M, Nova Biomedical, Waltham, MA).\nData are presented as mean ± sem; n denotes the number of experiments. Differences were considered significant when p \u003c 0.05."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T3049","span":{"begin":1019,"end":1024},"obj":"UBERON:0000178"},{"id":"T3048","span":{"begin":979,"end":981},"obj":"CHEBI:29103"},{"id":"T3047","span":{"begin":972,"end":978},"obj":"UBERON:0001969"},{"id":"T3046","span":{"begin":963,"end":970},"obj":"UBERON:0001844"},{"id":"T3045","span":{"begin":865,"end":868},"obj":"CHEBI:29101"},{"id":"T3044","span":{"begin":857,"end":860},"obj":"CHEBI:29101"},{"id":"T3043","span":{"begin":787,"end":790},"obj":"CHEBI:29101"},{"id":"T3042","span":{"begin":776,"end":778},"obj":"CHEBI:29103"},{"id":"T3041","span":{"begin":584,"end":587},"obj":"CHEBI:29101"},{"id":"T3040","span":{"begin":566,"end":568},"obj":"CHEBI:29103"},{"id":"T3039","span":{"begin":507,"end":510},"obj":"CHEBI:29101"},{"id":"T3038","span":{"begin":500,"end":502},"obj":"CHEBI:29103"},{"id":"T3037","span":{"begin":492,"end":498},"obj":"CHEBI:36916"},{"id":"T3036","span":{"begin":470,"end":479},"obj":"CHEBI:75958"},{"id":"T3035","span":{"begin":427,"end":429},"obj":"CHEBI:29103"},{"id":"T3034","span":{"begin":375,"end":391},"obj":"UBERON:0002282"},{"id":"T3033","span":{"begin":361,"end":365},"obj":"UBERON_EXT:bone_element_or_tissue"},{"id":"T3032","span":{"begin":302,"end":318},"obj":"UBERON:0002226"},{"id":"T3031","span":{"begin":161,"end":163},"obj":"CHEBI:29103"},{"id":"T3030","span":{"begin":146,"end":159},"obj":"UBERON:0001852"},{"id":"T3029","span":{"begin":83,"end":94},"obj":"CHEBI:26714"},{"id":"T3028","span":{"begin":39,"end":51},"obj":"CHEBI_EXT:anaesthetic_process"},{"id":"T3027","span":{"begin":26,"end":33},"obj":"NCBITaxon:33208"}],"text":"In situ electrophysiology\nAnimals were anesthetized with inactin (thiobutabarbital sodium salt, 140 mg/kg ip). The endocochlear potential and the endolymphatic [K+] were measured with double-barreled microelectrodes [17]. Measurements were made in the basal turn by a round-window approach through the basilar membrane and in the apical turn after thinning the bone over the stria vascularis and picking a small hole (~30 μm). K+-selective electrodes were calibrated in solutions of constant cation (K+ and Na+) concentration of 150 mM. The minor selectivity of the K+ electrodes for Na+ produced a nonlinearity in the calibration curve, which was closely fitted by the Nicolski equation using nonlinear curve-fitting software (OriginLab, Northampton, MA): V = Vi + S × log ([K+] + A × [Na+]), where Vi is an offset term, S is slope, A is selectivity, and [Na+] is Na+ concentration. Calibrations were made immediately after withdrawal of the electrodes from the cochlea. Plasma K+ concentrations were obtained using a blood analyzer (Stat Profile M, Nova Biomedical, Waltham, MA).\nData are presented as mean ± sem; n denotes the number of experiments. Differences were considered significant when p \u003c 0.05."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T3026","span":{"begin":1019,"end":1024},"obj":"UBERON:0000178"},{"id":"T3025","span":{"begin":979,"end":981},"obj":"CHEBI:29103"},{"id":"T3024","span":{"begin":972,"end":978},"obj":"UBERON:0001969"},{"id":"T3023","span":{"begin":963,"end":970},"obj":"UBERON:0001844"},{"id":"T3022","span":{"begin":865,"end":868},"obj":"CHEBI:29101"},{"id":"T3021","span":{"begin":857,"end":860},"obj":"CHEBI:29101"},{"id":"T3020","span":{"begin":787,"end":790},"obj":"CHEBI:29101"},{"id":"T3019","span":{"begin":776,"end":778},"obj":"CHEBI:29103"},{"id":"T3018","span":{"begin":584,"end":587},"obj":"CHEBI:29101"},{"id":"T3017","span":{"begin":566,"end":568},"obj":"CHEBI:29103"},{"id":"T3016","span":{"begin":507,"end":510},"obj":"CHEBI:29101"},{"id":"T3015","span":{"begin":500,"end":502},"obj":"CHEBI:29103"},{"id":"T3014","span":{"begin":492,"end":498},"obj":"CHEBI:36916"},{"id":"T3013","span":{"begin":470,"end":479},"obj":"CHEBI:75958"},{"id":"T3012","span":{"begin":427,"end":429},"obj":"CHEBI:29103"},{"id":"T3011","span":{"begin":375,"end":391},"obj":"UBERON:0002282"},{"id":"T3010","span":{"begin":302,"end":318},"obj":"UBERON:0002226"},{"id":"T3009","span":{"begin":161,"end":163},"obj":"CHEBI:29103"},{"id":"T3008","span":{"begin":146,"end":159},"obj":"UBERON:0001852"},{"id":"T3007","span":{"begin":83,"end":94},"obj":"CHEBI:26714"},{"id":"T3006","span":{"begin":26,"end":33},"obj":"NCBITaxon:33208"}],"text":"In situ electrophysiology\nAnimals were anesthetized with inactin (thiobutabarbital sodium salt, 140 mg/kg ip). The endocochlear potential and the endolymphatic [K+] were measured with double-barreled microelectrodes [17]. Measurements were made in the basal turn by a round-window approach through the basilar membrane and in the apical turn after thinning the bone over the stria vascularis and picking a small hole (~30 μm). K+-selective electrodes were calibrated in solutions of constant cation (K+ and Na+) concentration of 150 mM. The minor selectivity of the K+ electrodes for Na+ produced a nonlinearity in the calibration curve, which was closely fitted by the Nicolski equation using nonlinear curve-fitting software (OriginLab, Northampton, MA): V = Vi + S × log ([K+] + A × [Na+]), where Vi is an offset term, S is slope, A is selectivity, and [Na+] is Na+ concentration. Calibrations were made immediately after withdrawal of the electrodes from the cochlea. Plasma K+ concentrations were obtained using a blood analyzer (Stat Profile M, Nova Biomedical, Waltham, MA).\nData are presented as mean ± sem; n denotes the number of experiments. Differences were considered significant when p \u003c 0.05."}

{"project":"2_test","denotations":[{"id":"15320950-11788352-12070958","span":{"begin":217,"end":219},"obj":"11788352"}],"text":"In situ electrophysiology\nAnimals were anesthetized with inactin (thiobutabarbital sodium salt, 140 mg/kg ip). The endocochlear potential and the endolymphatic [K+] were measured with double-barreled microelectrodes [17]. Measurements were made in the basal turn by a round-window approach through the basilar membrane and in the apical turn after thinning the bone over the stria vascularis and picking a small hole (~30 μm). K+-selective electrodes were calibrated in solutions of constant cation (K+ and Na+) concentration of 150 mM. The minor selectivity of the K+ electrodes for Na+ produced a nonlinearity in the calibration curve, which was closely fitted by the Nicolski equation using nonlinear curve-fitting software (OriginLab, Northampton, MA): V = Vi + S × log ([K+] + A × [Na+]), where Vi is an offset term, S is slope, A is selectivity, and [Na+] is Na+ concentration. Calibrations were made immediately after withdrawal of the electrodes from the cochlea. Plasma K+ concentrations were obtained using a blood analyzer (Stat Profile M, Nova Biomedical, Waltham, MA).\nData are presented as mean ± sem; n denotes the number of experiments. Differences were considered significant when p \u003c 0.05."}