PMC:5118445 / 9741-10357 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/5118445","sourcedb":"PMC","sourceid":"5118445","source_url":"https://www.ncbi.nlm.nih.gov/pmc/5118445","text":"Table 1 summarizes the results of immunofluorescence staining for NET components and neutrophil marker proteins under different antigen retrieval conditions. Figure 1 shows immunofluorescence images resulting from different antigen retrieval protocols. Immunodetection of NE is dependent on the incubation temperature, which may not exceed 60°C (Figure 1C). Staining is stronger at pH6 compared to pH9. The cytoplasmic NET component calprotectin (3), a heterodimer consisting of Calgranulin A and B, is readily detected at all temperatures tested. As expected, the staining patterns for both subunits did not differ.","tracks":[{"project":"TEST0","denotations":[{"id":"27920776-44-49-3175572","span":{"begin":447,"end":448},"obj":"[\"19876394\"]"}],"attributes":[{"subj":"27920776-44-49-3175572","pred":"source","obj":"TEST0"}]},{"project":"2_test","denotations":[{"id":"27920776-19876394-34775611","span":{"begin":447,"end":448},"obj":"19876394"}],"attributes":[{"subj":"27920776-19876394-34775611","pred":"source","obj":"2_test"}]},{"project":"MyTest","denotations":[{"id":"27920776-19876394-34775611","span":{"begin":447,"end":448},"obj":"19876394"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"attributes":[{"subj":"27920776-19876394-34775611","pred":"source","obj":"MyTest"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"TEST0","color":"#cdec93","default":true},{"id":"2_test","color":"#ec93e7"},{"id":"MyTest","color":"#93ecd6"}]}]}}