PMC:5118421 / 15920-16828 JSONTXT

{"project":"TEST0","denotations":[{"id":"27920779-144-150-3100228","span":{"begin":497,"end":499},"obj":"[\"16497590\"]"},{"id":"27920779-148-154-3100229","span":{"begin":501,"end":503},"obj":"[\"16623761\"]"},{"id":"27920779-90-96-3100230","span":{"begin":904,"end":906},"obj":"[\"8738915\"]"}],"text":"The frequent use of CDR AGY Ser codons among IgV-region genes from two different species (human and mouse) led us to speculate that this feature might be highly conserved in evolution. Thus, we analyzed IgVH gene sequences of cartilaginous fishes (class Chondrichthyes), which are descendants of the most ancient species with an adaptive immune system. The immune systems of species in this class share major features with those of mammals, including SHM, although not class switch recombination (32, 33). Our analysis of germline VH sequences from four Chondrichthyes species indicated that, as in mice and humans, AGY but not TCN Ser codons were enriched in germline-encoded CDR sequences (Figures S2A,B in Supplementary Material). Thus, the CDR AGY codon bias is a highly conserved feature of IgV-region genes. A similar trend was also observed in several other less distant species, by Jolly et al. (18)."}

{"project":"2_test","denotations":[{"id":"27920779-16497590-34707951","span":{"begin":497,"end":499},"obj":"16497590"},{"id":"27920779-16623761-34707952","span":{"begin":501,"end":503},"obj":"16623761"},{"id":"27920779-8738915-34707953","span":{"begin":904,"end":906},"obj":"8738915"}],"text":"The frequent use of CDR AGY Ser codons among IgV-region genes from two different species (human and mouse) led us to speculate that this feature might be highly conserved in evolution. Thus, we analyzed IgVH gene sequences of cartilaginous fishes (class Chondrichthyes), which are descendants of the most ancient species with an adaptive immune system. The immune systems of species in this class share major features with those of mammals, including SHM, although not class switch recombination (32, 33). Our analysis of germline VH sequences from four Chondrichthyes species indicated that, as in mice and humans, AGY but not TCN Ser codons were enriched in germline-encoded CDR sequences (Figures S2A,B in Supplementary Material). Thus, the CDR AGY codon bias is a highly conserved feature of IgV-region genes. A similar trend was also observed in several other less distant species, by Jolly et al. (18)."}

{"project":"MyTest","denotations":[{"id":"27920779-16497590-34707951","span":{"begin":497,"end":499},"obj":"16497590"},{"id":"27920779-16623761-34707952","span":{"begin":501,"end":503},"obj":"16623761"},{"id":"27920779-8738915-34707953","span":{"begin":904,"end":906},"obj":"8738915"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"text":"The frequent use of CDR AGY Ser codons among IgV-region genes from two different species (human and mouse) led us to speculate that this feature might be highly conserved in evolution. Thus, we analyzed IgVH gene sequences of cartilaginous fishes (class Chondrichthyes), which are descendants of the most ancient species with an adaptive immune system. The immune systems of species in this class share major features with those of mammals, including SHM, although not class switch recombination (32, 33). Our analysis of germline VH sequences from four Chondrichthyes species indicated that, as in mice and humans, AGY but not TCN Ser codons were enriched in germline-encoded CDR sequences (Figures S2A,B in Supplementary Material). Thus, the CDR AGY codon bias is a highly conserved feature of IgV-region genes. A similar trend was also observed in several other less distant species, by Jolly et al. (18)."}