PMC:5115869 / 14421-15171 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/5115869","sourcedb":"PMC","sourceid":"5115869","source_url":"https://www.ncbi.nlm.nih.gov/pmc/5115869","text":"Figure 2—figure supplement 1. Specific fcAMP binding at single molecules.\n(A–C) Single-molecule fluorescence time series for individual acceptor-labeled CNBDs in the absence (A) and presence (B) of 3 µM fcAMP, and during competition between 3 µM fcAMP and 5 mM non-fluorescent cAMP (C). Simultaneous emission from donor (blue) and acceptor (red) upon donor excitation at 532 nm was interleaved every other frame with emission from acceptor (magenta) upon direct acceptor excitation at 640 nm. The lack of smFRET events during competition with cAMP indicate a lack of non-specific binding. (D) Summary of the binding event rate from smFRET traces across molecules for the same conditions described above.\nDOI: http://dx.doi.org/10.7554/eLife.20797.007","divisions":[{"label":"label","span":{"begin":0,"end":29}},{"label":"title","span":{"begin":30,"end":73}},{"label":"p","span":{"begin":74,"end":703}}],"tracks":[]}