PMC:5113056 / 6211-6942 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/5113056","sourcedb":"PMC","sourceid":"5113056","source_url":"https://www.ncbi.nlm.nih.gov/pmc/5113056","text":"Total RNA was prepared from cell cultures that were positive for SVCV using the Ambion MagMAX Viral RNA Isolation Kit according to the manufacturer's instructions. Samples were sequenced at the National Veterinary Services Laboratories (Ames, IA, USA) on an Ion Torrent PGM instrument (Life Technologies, Carlsbad, CA, USA) using Ion Total RNA-Seq (v2) chemistries as per the manufacturer's instructions. Sequencing reads were assembled against reference sequences (GenBank KJ513477 and DQ491000) selected from results of BLAST analysis of a 2500-nt fragment generated by Sanger sequence analysis14 of isolate salamander/USA/202238/2015 (hereafter 202238). The assembled sequence has been deposited in GenBank (accession KU230365).","tracks":[{"project":"2_test","denotations":[{"id":"27599472-17048110-27784057","span":{"begin":596,"end":598},"obj":"17048110"}],"attributes":[{"subj":"27599472-17048110-27784057","pred":"source","obj":"2_test"}]},{"project":"MyTest","denotations":[{"id":"27599472-17048110-27784057","span":{"begin":596,"end":599},"obj":"17048110"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"attributes":[{"subj":"27599472-17048110-27784057","pred":"source","obj":"MyTest"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec93b8","default":true},{"id":"MyTest","color":"#93d2ec"}]}]}}