PMC:5113056 / 11022-12992 JSONTXT

{"project":"2_test","denotations":[{"id":"27599472-12691191-27784069","span":{"begin":167,"end":169},"obj":"12691191"},{"id":"27599472-26717888-27784070","span":{"begin":1887,"end":1889},"obj":"26717888"}],"text":"CHARACTERIZATION OF THE SALAMANDER ISOLATES\nTo further characterize the nine viral isolates, a 714-nt fragment of the viral glycoprotein (G) gene was amplified by PCR.12 The amplified G gene fragments exhibited 100% identity to one another over the region sequenced. One viral isolate (202238) was also chosen for whole-genome sequencing. A near complete contig of 10 991 nt that encoded five genes (nucleoprotein, N; phosphoprotein, P; matrix, M; glycoprotein, G; and large polymerase, L) in an order that is characteristic of the genus Sprivivirus was obtained following assembly of the genome (GenBank accession KU230365, Figure 2). There was no nonvirion gene at the G–L junction as would be characteristic of vesiculoviruses and perhabdoviruses. The conserved transcriptional start signal of 3′-UUGUC-5′ and termination/polyadenylation signal of 3′-AUA CUU UUU UU-5′ were both present in all five genes, and their presence is characteristic (although not exclusively) of Sprivivirus.27 Overall, the salamander virus was assigned to SVCV in accordance with the International Committee on Taxonomy of Viruses (ICTV).27\nBayesian and maximum likelihood phylogenetic analyses of the G gene fragments indicated that the salamander isolates clustered most closely with SVCV genogroup I (Figure 1). Median-joining network analysis further classified the virus to genogroup Ia (Figure 2). The salamander SVCV was distinct from previous isolates of SVCV from North America but was closely related to recent isolates from China. Although relatively few whole-genome sequences for SVCV are available for comparison, the salamander SVCV protein coding regions (N, P, M, G and L) were all closely related (98.2%–99.8% identical) at the nucleotide and amino-acid levels to corresponding genes in SVCV strain SH140501 (Table 2), which was isolated from a goldfish in Shanghai sampled in April 2014.28 This finding further supported an Asian origin for the salamander SVCV isolates."}

{"project":"MyTest","denotations":[{"id":"27599472-12691191-27784069","span":{"begin":167,"end":170},"obj":"12691191"},{"id":"27599472-26717888-27784070","span":{"begin":1887,"end":1890},"obj":"26717888"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"text":"CHARACTERIZATION OF THE SALAMANDER ISOLATES\nTo further characterize the nine viral isolates, a 714-nt fragment of the viral glycoprotein (G) gene was amplified by PCR.12 The amplified G gene fragments exhibited 100% identity to one another over the region sequenced. One viral isolate (202238) was also chosen for whole-genome sequencing. A near complete contig of 10 991 nt that encoded five genes (nucleoprotein, N; phosphoprotein, P; matrix, M; glycoprotein, G; and large polymerase, L) in an order that is characteristic of the genus Sprivivirus was obtained following assembly of the genome (GenBank accession KU230365, Figure 2). There was no nonvirion gene at the G–L junction as would be characteristic of vesiculoviruses and perhabdoviruses. The conserved transcriptional start signal of 3′-UUGUC-5′ and termination/polyadenylation signal of 3′-AUA CUU UUU UU-5′ were both present in all five genes, and their presence is characteristic (although not exclusively) of Sprivivirus.27 Overall, the salamander virus was assigned to SVCV in accordance with the International Committee on Taxonomy of Viruses (ICTV).27\nBayesian and maximum likelihood phylogenetic analyses of the G gene fragments indicated that the salamander isolates clustered most closely with SVCV genogroup I (Figure 1). Median-joining network analysis further classified the virus to genogroup Ia (Figure 2). The salamander SVCV was distinct from previous isolates of SVCV from North America but was closely related to recent isolates from China. Although relatively few whole-genome sequences for SVCV are available for comparison, the salamander SVCV protein coding regions (N, P, M, G and L) were all closely related (98.2%–99.8% identical) at the nucleotide and amino-acid levels to corresponding genes in SVCV strain SH140501 (Table 2), which was isolated from a goldfish in Shanghai sampled in April 2014.28 This finding further supported an Asian origin for the salamander SVCV isolates."}