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craft-ca-core-ex-dev

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Id Subject Object Predicate Lexical cue
T577 246-254 GO:0065007 denotes regulate
T659 1608-1619 NCBITaxon:7742 denotes vertebrates
T565 4-13 NCBITaxon:7955 denotes Zebrafish
T566 14-23 PR_EXT:Q6E2N3 denotes moonshine
T567 24-28 SO_EXT:0000704 denotes Gene
T568 29-36 SO_EXT:sequence_coding_function denotes Encodes
T569 37-52 GO_EXT:transcription denotes Transcriptional
T570 37-75 PR_EXT:000016655 denotes Transcriptional Intermediary Factor 1γ
T571 90-99 GO_EXT:regulator denotes Regulator
T572 103-116 GO:0030097 denotes Hematopoiesis
T573 160-173 GO:0030097 denotes Hematopoiesis
T574 220-223 CHEBI_SO_EXT:DNA denotes DNA
T575 224-231 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T576 232-240 CHEBI_PR_EXT:protein denotes proteins
T578 255-268 GO_EXT:transcription denotes transcription
T579 316-325 SO_EXT:sequence_alteration_entity_or_process denotes Mutations
T580 333-342 NCBITaxon:7955 denotes zebrafish
T581 343-352 PR_EXT:Q6E2N3 denotes moonshine
T582 354-357 PR_EXT:Q6E2N3 denotes mon
T583 359-363 SO_EXT:0000704 denotes gene
T584 390-399 UBERON:0000922 denotes embryonic
T585 390-399 _FRAGMENT denotes embryonic
T586 410-423 GO:0035162 denotes hematopoiesis
T587 404-409 UBERON:0007023 denotes adult
T588 445-459 CL:0000232 denotes red blood cell
T589 449-454 UBERON:0000178 denotes blood
T590 455-459 CL_GO_EXT:cell denotes cell
T591 484-487 PR_EXT:Q6E2N3 denotes mon
T592 488-495 SO_EXT:sequence_coding_function denotes encodes
T593 500-509 NCBITaxon:7955 denotes zebrafish
T594 510-518 SO_EXT:0000855 denotes ortholog
T595 522-531 NCBITaxon:40674 denotes mammalian
T596 532-547 GO_EXT:transcription denotes transcriptional
T597 532-570 PR_EXT:000016655 denotes transcriptional intermediary factor 1γ
T598 572-577 PR_EXT:000016655 denotes TIF1γ
T599 583-589 PR_EXT:000016655 denotes TRIM33
T600 608-612 PR_EXT:TIF1 denotes TIF1
T601 674-687 GO:0030097 denotes hematopoietic
T602 674-704 CL:0008001 denotes hematopoietic progenitor cells
T603 699-704 CL_GO_EXT:cell denotes cells
T604 708-711 PR_EXT:Q6E2N3 denotes mon
T605 712-719 SO_EXT:sequence_altered_entity denotes mutants
T606 728-735 GO:0010467 denotes express
T607 753-766 GO:0030097 denotes hematopoietic
T608 767-780 GO_EXT:transcription denotes transcription
T609 767-788 GO_EXT:transcription_factor denotes transcription factors
T610 800-805 PR_EXT:000007857 denotes gata1
T611 819-828 GO:0006915 denotes apoptosis
T612 846-849 PR_EXT:Q6E2N3 denotes mon
T613 850-856 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T614 857-864 SO_EXT:0001023 denotes alleles
T615 870-876 SO_EXT:sequence_coding_function denotes encode
T616 877-898 SO_EXT:0001587 denotes premature stop codons
T617 913-923 GO:0010467 denotes expression
T618 927-936 SO_EXT:wild_type_entity_or_quality denotes wild-type
T619 937-942 PR_EXT:000016655 denotes tif1γ
T620 943-947 CHEBI_SO_EXT:mRNA denotes mRNA
T621 948-955 SO_EXT:sequence_rescue_process denotes rescues
T622 956-965 UBERON:0000922 denotes embryonic
T623 956-979 GO:0035162 denotes embryonic hematopoiesis
T624 994-997 PR_EXT:Q6E2N3 denotes mon
T625 998-1005 SO_EXT:sequence_altered_entity denotes mutants
T626 1037-1044 CL:0000365 denotes zygotic
T627 1045-1050 PR_EXT:000016655 denotes tif1γ
T628 1051-1055 CHEBI_SO_EXT:mRNA denotes mRNA
T630 1078-1094 UBERON:2000083 denotes ventral mesoderm
T631 1102-1115 GO:0030097 denotes hematopoietic
T632 1102-1125 CL:0000037 denotes hematopoietic stem cell
T633 1121-1125 CL_GO_EXT:cell denotes cell
T634 1121-1125 _FRAGMENT denotes cell
T635 1141-1150 GO:0048468 denotes formation
T636 1160-1165 PR_EXT:000007857 denotes gata1
T637 1166-1176 GO:0010467 denotes expression
T638 1214-1219 PR_EXT:000016655 denotes tif1γ
T639 1235-1239 CL_GO_EXT:cell denotes cell
T640 1269-1284 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T641 1288-1297 CL:0000764 denotes erythroid
T642 1321-1327 NCBITaxon:39107 denotes murine
T643 1328-1342 CL:0000764 denotes erythroid cell
T644 1338-1342 CL_GO_EXT:cell denotes cell
T645 1366-1371 PR_EXT:000016655 denotes Tif1γ
T646 1372-1379 CHEBI_PR_EXT:protein denotes protein
T647 1383-1392 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T648 1406-1413 GO:0005634 denotes nuclear
T649 1424-1434 GO:0010467 denotes expression
T650 1452-1466 CL:0000764 denotes erythroid cell
T651 1462-1466 CL_GO_EXT:cell denotes cell
T652 1462-1477 GO:0048469 denotes cell maturation
T653 1523-1538 GO_EXT:transcription denotes transcriptional
T654 1566-1584 _FRAGMENT denotes differentiation of
T655 1599-1604 GO:0030154 denotes cells
T656 1585-1598 GO:0030097 denotes hematopoietic
T657 1585-1604 CL:0000988 denotes hematopoietic cells
T658 1599-1604 CL_GO_EXT:cell denotes cells
T2510 1812-1825 GO:0030097 denotes Hematopoiesis
T2511 1864-1868 CL_GO_EXT:cell denotes cell
T2512 1864-1882 GO:0008283 denotes cell proliferation
T2513 1887-1905 _FRAGMENT denotes differentiation of
T2514 1946-1951 GO:0030154 denotes cells
T2515 1946-1951 CL_GO_EXT:cell denotes cells
T2516 1969-1980 _FRAGMENT denotes circulating
T2517 2001-2006 CL:0000080 denotes cells
T2518 1981-1984 _FRAGMENT denotes red
T2519 1995-2006 CL:0000232 denotes blood cells
T2520 1989-2006 CL:0000738 denotes white blood cells
T2521 1995-2000 UBERON:0000178 denotes blood
T2522 2001-2006 CL_GO_EXT:cell denotes cells
T2523 2030-2043 GO:0030097 denotes Hematopoiesis
T2524 2047-2058 NCBITaxon:7742 denotes vertebrates
T2525 2065-2074 NCBITaxon:7955 denotes zebrafish
T2526 2078-2084 NCBITaxon:9606 denotes humans
T2527 2092-2116 SO_EXT:biological_conservation_process_or_quality denotes evolutionarily conserved
T2528 2152-2156 _FRAGMENT denotes stem
T2529 2171-2176 CL:0000034 denotes cells
T2530 2171-2176 CL_GO_EXT:cell denotes cells
T2531 2203-2212 UBERON:0000922 denotes embryonic
T2532 2244-2258 GO_PATO_RO_EXT:developmental_differentiation_process_or_quality denotes differentiated
T2533 2259-2264 UBERON:0000178 denotes blood
T2534 2259-2270 CL:0000081 denotes blood cells
T2535 2265-2270 CL_GO_EXT:cell denotes cells
T2536 2380-2393 GO:0030097 denotes hematopoiesis
T2537 2410-2426 UBERON:2000083 denotes ventral mesoderm
T2538 2456-2461 CL_GO_EXT:cell denotes cells
T2539 2467-2480 GO_RO_EXT:developmental_differentiation_process denotes differentiate
T2540 2484-2493 UBERON:0000922 denotes embryonic
T2541 2494-2507 UBERON:0003061 denotes blood islands
T2542 2531-2544 GO:0030097 denotes hematopoiesis
T2543 2565-2574 UBERON:0000922 denotes embryonic
T2544 2575-2587 CL:0000232 denotes erythrocytes
T2545 2592-2603 CL:0000235 denotes macrophages
T2546 2640-2653 GO:0030097 denotes hematopoiesis
T2547 2680-2690 CL:0000034 denotes stem cells
T2548 2685-2690 CL_GO_EXT:cell denotes cells
T2549 2726-2735 UBERON:0000922 denotes embryonic
T2550 2736-2741 UBERON:0000947 denotes aorta
T2551 2742-2747 UBERON:0000991 denotes gonad
T2552 2748-2759 UBERON:0000080 denotes mesonephros
T2553 2774-2798 GO:0060216 denotes definitive hematopoietic
T2554 2785-2809 CL:0000037 denotes hematopoietic stem cells
T2555 2804-2809 CL_GO_EXT:cell denotes cells
T2556 2878-2883 UBERON:0007023 denotes adult
T2557 2884-2889 UBERON:0000178 denotes blood
T2558 2884-2895 CL:0000081 denotes blood cells
T2559 2890-2895 CL_GO_EXT:cell denotes cells
T2560 2918-2930 CL:0000232 denotes erythrocytes
T2561 2932-2945 CL:0000763 denotes myeloid cells
T2562 2940-2945 CL_GO_EXT:cell denotes cells
T2563 2951-2962 CL:0000542 denotes lymphocytes
T2564 2985-2992 SO_EXT:0000704 denotes genetic
T2565 3018-3023 SO_EXT:0000704 denotes genes
T2566 3029-3036 GO:0065007 denotes control
T2567 3037-3050 GO:0030097 denotes hematopoiesis
T2568 3061-3070 NCBITaxon:7955 denotes zebrafish
T2569 3145-3152 SO_EXT:0000704 denotes genetic
T2570 3186-3191 UBERON:0000178 denotes blood
T2571 3196-3205 NCBITaxon:7955 denotes zebrafish
T2572 3206-3213 SO_EXT:sequence_altered_entity denotes mutants
T2573 3229-3236 GO:0010467 denotes express
T2574 3241-3250 CL:0000764 denotes erythroid
T2575 3251-3264 GO_EXT:transcription denotes transcription
T2576 3251-3271 GO_EXT:transcription_factor denotes transcription factor
T2577 3272-3277 PR_EXT:000007857 denotes gata1
T2578 3290-3299 UBERON:0000922 denotes embryonic
T2579 3290-3313 GO:0035162 denotes embryonic hematopoietic
T2580 3369-3378 NCBITaxon:7955 denotes zebrafish
T2581 3379-3384 SO_EXT:0000704 denotes genes
T2582 3385-3394 PR_EXT:Q6E2N3 denotes moonshine
T2583 3396-3399 PR_EXT:Q6E2N3 denotes mon
T2584 3445-3451 SO_EXT:0001023 denotes allele
T2585 3515-3524 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T2586 3532-3535 PR_EXT:Q6E2N3 denotes mon
T2587 3536-3540 SO_EXT:0000704 denotes gene
T2588 3568-3587 _FRAGMENT denotes primitive embryonic
T2589 3609-3622 GO:0060215 denotes hematopoiesis
T2590 3578-3587 UBERON:0000922 denotes embryonic
T2591 3592-3602 _FRAGMENT denotes definitive
T2592 3609-3622 GO:0060216 denotes hematopoiesis
T2593 3603-3608 UBERON:0007023 denotes adult
T2594 3654-3669 CL:0000764 denotes erythroid cells
T2595 3664-3669 CL_GO_EXT:cell denotes cells
T2596 3671-3697 CL:0000038 denotes Erythroid progenitor cells
T2597 3692-3697 CL_GO_EXT:cell denotes cells
T2598 3701-3704 PR_EXT:Q6E2N3 denotes mon
T2599 3705-3712 SO_EXT:sequence_altered_entity denotes mutants
T2600 3748-3755 GO:0010467 denotes express
T2601 3773-3786 GO:0030097 denotes hematopoietic
T2602 3787-3800 GO_EXT:transcription denotes transcription
T2603 3787-3808 GO_EXT:transcription_factor denotes transcription factors
T2604 3821-3830 GO:0006915 denotes apoptosis
T2605 3843-3850 SO_EXT:sequence_cloning_process denotes cloning
T2606 3866-3869 PR_EXT:Q6E2N3 denotes mon
T2607 3870-3874 SO_EXT:0000704 denotes gene
T2608 3882-3891 NCBITaxon:7955 denotes zebrafish
T2609 3892-3900 SO_EXT:0000855 denotes ortholog
T2610 3904-3913 NCBITaxon:40674 denotes mammalian
T2611 3914-3929 GO_EXT:transcription denotes transcriptional
T2612 3914-3952 PR_EXT:000016655 denotes transcriptional intermediary factor 1γ
T2613 3954-3959 PR_EXT:000016655 denotes TIF1γ
T2614 3978-3982 PR_EXT:TIF1 denotes TIF1
T2615 3993-4008 GO_EXT:transcription denotes transcriptional
T2616 3993-4021 GO_EXT:0003713 denotes transcriptional coactivators
T2617 3993-4008 _FRAGMENT denotes transcriptional
T2618 4026-4038 GO_EXT:0003714 denotes corepressors
T2619 4170-4180 NCBITaxon:7742 denotes vertebrate
T2620 4181-4185 PR_EXT:TIF1 denotes TIF1
T2621 4194-4195 PR_EXT:000016649 denotes α
T2622 4197-4198 PR_EXT:000016652 denotes β
T2623 4204-4205 PR_EXT:000016655 denotes γ
T2624 4217-4224 GO:0005634 denotes nuclear
T2625 4225-4233 CHEBI_PR_EXT:protein denotes proteins
T2626 4255-4265 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes N-terminal
T2627 4279-4285 SO_EXT:0000417 denotes domain
T2628 4354-4365 SO_EXT:0001080 denotes coiled-coil
T2629 4366-4372 SO_EXT:0000417 denotes domain
T2630 4374-4378 PR_EXT:TIF1 denotes TIF1
T2631 4409-4419 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T2632 4420-4425 NCBITaxon:3193 denotes plant
T2633 4488-4497 GO:0000785 denotes chromatin
T2634 4488-4508 GO:0006338 denotes chromatin remodeling
T2635 4518-4523 PR_EXT:000016649 denotes TIF1α
T2636 4570-4576 CHEBI_GO_EXT:ligand denotes ligand
T2637 4577-4582 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes bound
T2638 4583-4590 GO:0005634 denotes nuclear
T2639 4591-4598 CHEBI_GO_EXT:hormone denotes hormone
T2640 4599-4608 GO_EXT:0004872 denotes receptors
T2641 4668-4681 CHEBI:26536 denotes retinoic acid
T2642 4668-4691 GO_EXT:0003708 denotes retinoic acid receptors
T2643 4712-4717 PR_EXT:000016652 denotes TIF1β
T2644 4811-4817 SO_EXT:0000417 denotes domain
T2645 4818-4822 CHEBI_EXT:zinc denotes zinc
T2646 4830-4843 GO_EXT:transcription denotes transcription
T2647 4830-4851 GO_EXT:transcription_factor denotes transcription factors
T2648 4909-4914 PR_EXT:000016655 denotes TIF1γ
T2649 4955-4962 GO:0005634 denotes nuclear
T2650 4963-4972 GO_EXT:0004872 denotes receptors
T2651 4981-4988 SO_EXT:0000417 denotes domains
T2652 4994-4998 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes bind
T2653 5012-5016 PR_EXT:TIF1 denotes TIF1
T2654 5077-5088 CHEBI_EXT:biochemical denotes Biochemical
T2655 5119-5124 PR_EXT:000016655 denotes TIF1γ
T2656 5141-5150 GO_EXT:oligomeric_macromolecular_complex denotes oligomers
T2657 5162-5171 GO_EXT:oligomeric_macromolecular_complex denotes oligomers
T2658 5177-5182 PR_EXT:000016649 denotes TIF1α
T2659 5196-5201 PR_EXT:000016652 denotes TIF1β
T2660 5226-5232 NCBITaxon:39107 denotes murine
T2661 5233-5238 PR_EXT:000016649 denotes Tif1α
T2662 5243-5248 PR_EXT:000016655 denotes Tif1γ
T2663 5249-5254 SO_EXT:0000704 denotes genes
T2664 5286-5290 SO_EXT:0000704 denotes gene
T2665 5334-5339 NCBITaxon:10088 denotes mouse
T2666 5340-5347 SO_EXT:sequence_altered_entity denotes mutants
T2667 5366-5371 PR_EXT:000016652 denotes Tif1β
T2668 5392-5404 GO:0007566 denotes implantation
T2669 5405-5418 GO:0009790 denotes embryogenesis
T2670 5423-5431 UBERON:0000926 denotes mesoderm
T2671 5541-5545 PR_EXT:TIF1 denotes TIF1
T2672 5572-5575 CHEBI_SO_EXT:DNA denotes DNA
T2673 5576-5583 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T2674 5584-5592 CHEBI_PR_EXT:protein denotes proteins
T2675 5680-5685 PR_EXT:000016655 denotes tif1γ
T2676 5712-5721 GO_EXT:regulator denotes regulator
T2677 5725-5734 UBERON:0000922 denotes embryonic
T2678 5725-5734 _FRAGMENT denotes embryonic
T2679 5745-5758 GO:0035162 denotes hematopoiesis
T2680 5739-5744 UBERON:0007023 denotes adult
T2681 5762-5773 NCBITaxon:7742 denotes vertebrates
T2682 5775-5779 CL_GO_EXT:cell denotes Cell
T2683 5821-5826 PR_EXT:000016655 denotes tif1γ
T2684 5837-5841 CL_GO_EXT:cell denotes cell
T2685 5867-5876 UBERON:0000922 denotes embryonic
T2686 5867-5890 GO:0035162 denotes embryonic hematopoiesis
T2687 5896-5901 PR_EXT:000016655 denotes tif1γ
T2688 5902-5906 SO_EXT:0000704 denotes gene
T2689 5910-5919 GO:0010467 denotes expressed
T2690 5936-5952 UBERON:2000083 denotes ventral mesoderm
T2691 5957-5970 GO:0030097 denotes hematopoietic
T2692 5989-6002 GO_EXT:negative_regulation denotes downregulated
T2693 6006-6015 CL:0000764 denotes erythroid
T2694 6016-6026 GO_PATO_EXT:biological_maturation denotes maturation
T2695 6035-6040 PR_EXT:000016655 denotes Tif1γ
T2696 6041-6048 CHEBI_PR_EXT:protein denotes protein
T2697 6049-6058 GO_PATO_EXT:biological_localization_process_or_quality denotes localizes
T2698 6079-6093 GO:0016604 denotes nuclear bodies
T2699 6110-6115 NCBITaxon:10088 denotes mouse
T2700 6116-6122 UBERON:0000922 denotes embryo
T2701 6123-6134 CL:0000057 denotes fibroblasts
T2702 6155-6159 CL_GO_EXT:cell denotes cell
T2703 6212-6217 PR_EXT:000016655 denotes Tif1γ
T2704 6241-6255 CL:0000764 denotes erythroid cell
T2705 6251-6255 CL_GO_EXT:cell denotes cell
T2706 6251-6267 GO:0048468 denotes cell development
T4506 4-13 NCBITaxon:7955 denotes Zebrafish
T4507 14-6309 PR_EXT:Q6E2N3 denotes moonshine Gene Encodes Transcriptional Intermediary Factor 1γ, an Essential Regulator of Hematopoiesis moonshine/tif1γ in Hematopoiesis Abstract Hematopoiesis is precisely orchestrated by lineage-specific DNA-binding proteins that regulate transcription in concert with coactivators and corepressors. Mutations in the zebrafish moonshine (mon) gene specifically disrupt both embryonic and adult hematopoiesis, resulting in severe red blood cell aplasia. We report that mon encodes the zebrafish ortholog of mammalian transcriptional intermediary factor 1γ (TIF1γ) (or TRIM33), a member of the TIF1 family of coactivators and corepressors. During development, hematopoietic progenitor cells in mon mutants fail to express normal levels of hematopoietic transcription factors, including gata1, and undergo apoptosis. Three different mon mutant alleles each encode premature stop codons, and enforced expression of wild-type tif1γ mRNA rescues embryonic hematopoiesis in homozygous mon mutants. Surprisingly, a high level of zygotic tif1γ mRNA expression delineates ventral mesoderm during hematopoietic stem cell and progenitor formation prior to gata1 expression. Transplantation studies reveal that tif1γ functions in a cell-autonomous manner during the differentiation of erythroid precursors. Studies in murine erythroid cell lines demonstrate that Tif1γ protein is localized within novel nuclear foci, and expression decreases during erythroid cell maturation. Our results establish a major role for this transcriptional intermediary factor in the differentiation of hematopoietic cells in vertebrates. A new gene acting early in red blood cell development is discovered by genetic analysis in zebrafish. The gene encodes a member of a well-known family of transcription factors Introduction Hematopoiesis involves the coordinated processes of cell proliferation and differentiation of a relatively small number of progenitor cells into billions of circulating red and white blood cells (Thisse and Zon 2002). Hematopoiesis in vertebrates, from zebrafish to humans, is an evolutionarily conserved program that produces two waves of stem or progenitor cells that differ both in their embryonic origins and in the lineages of differentiated blood cells produced (Palis and Yoder 2001; Orkin and Zon 2002; Galloway and Zon 2003). The first, or primitive, wave of hematopoiesis originates from ventral mesoderm and gives rise to progenitor cells that differentiate in embryonic blood islands. The primitive wave of hematopoiesis produces a burst of embryonic erythrocytes and macrophages. The second, or definitive, wave of hematopoiesis arises from self-renewing stem cells that develop primarily in the intraembryonic aorta–gonad–mesonephros region. These definitive hematopoietic stem cells seed the later developing marrow spaces, to produce all lineages of adult blood cells, including definitive erythrocytes, myeloid cells, and lymphocytes. We have undertaken a genetic approach to characterize genes that control hematopoiesis using the zebrafish as a model system (Thisse and Zon 2002). As part of a large-scale forward genetic screen, we previously identified bloodless zebrafish mutants that failed to express the erythroid transcription factor gata1 normally in embryonic hematopoietic precursors (Ransom et al. 1996). We named one of these zebrafish genes moonshine (mon), and another group named a noncomplementing allele vampire (Weinstein et al. 1996). Here, we have determined that mutations in the mon gene cause a disruption in both primitive embryonic and definitive adult hematopoiesis, resulting in a severe loss of erythroid cells. Erythroid progenitor cells in mon mutants are initially present, but fail to express normal levels of hematopoietic transcription factors and undergo apoptosis. Positional cloning identifies the mon gene as the zebrafish ortholog of mammalian transcriptional intermediary factor 1γ (TIF1γ), a member of the TIF1 family of transcriptional coactivators and corepressors (Le Douarin et al. 1995; Friedman et al. 1996; Kim et al. 1996; Venturini et al. 1999; Peng et al. 2002). The three members of the vertebrate TIF1 family (α, β, and γ) are large nuclear proteins that each contain an N-terminal RBCC or TRIM domain (Reymond et al. 2001) composed of a RING finger, two B-boxes, and a coiled-coil domain. TIF1 family members also contain a C-terminal plant homeodomain finger and bromodomain that are characteristic of chromatin remodeling factors. TIF1α has been shown to associate with a variety of ligand-bound nuclear hormone receptors (Le Douarin et al. 1995) and function as a coactivator for retinoic acid receptors (Zhong et al.1999). TIF1β has been shown to act as a corepressor for the large family of Krüppel-associated box (KRAB) domain zinc-finger transcription factors (Friedman et al. 1996; Abrink et al. 2001). In contrast, TIF1γ does not associate directly with either nuclear receptors or KRAB domains that bind to the other TIF1 family members (Venturini et al. 1999; Abrink et al. 2001). Biochemical studies also demonstrate that TIF1γ forms both homo-oligomers and hetero-oligomers with TIF1α but not with TIF1β (Peng et al. 2002). The murine Tif1α and Tif1γ genes have not yet been subjected to gene targeting experiments, whereas analysis of mouse mutants demonstrates that Tif1β is required for postimplantation embryogenesis and mesoderm induction in particular (Cammas et al. 2000). Taken together, these studies suggest that a major function of TIF1 family members is to link DNA-binding proteins with other coactivators or corepressors during development. Our studies establish that tif1γ functions as an essential regulator of embryonic and adult hematopoiesis in vertebrates. Cell transplantation studies demonstrate that tif1γ acts in a cell-autonomous manner during embryonic hematopoiesis. The tif1γ gene is expressed specifically in ventral mesoderm and hematopoietic progenitors, then downregulated as erythroid maturation occurs. Tif1γ protein localizes to a novel class of nuclear bodies in both primary mouse embryo fibroblasts and erythroleukemia cell lines. Taken together, our studies demonstrate that Tif1γ is required for normal erythroid cell development and survival. Results The Zebrafish mon
T4508 6310-6314 SO_EXT:0000704 denotes Gene
T4509 6337-6346 _FRAGMENT denotes Primitive
T4510 6362-6376 GO:0060319 denotes Erythropoiesis
T4511 6351-6376 GO:0060318 denotes Definitive Erythropoiesis
T4512 6408-6411 PR_EXT:Q6E2N3 denotes mon
T4513 6412-6416 SO_EXT:0000704 denotes gene
T4514 6463-6476 GO:0030097 denotes hematopoietic
T4515 6477-6481 SO_EXT:0000704 denotes gene
T4516 6477-6492 GO:0010467 denotes gene expression
T4517 6497-6506 GO:0006915 denotes apoptosis
T4518 6510-6519 NCBITaxon:7955 denotes zebrafish
T4519 6531-6534 PR_EXT:Q6E2N3 denotes mon
T4520 6535-6541 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T4521 6542-6549 UBERON:0000922 denotes embryos
T4522 6558-6571 GO:0009790 denotes embryogenesis
T4523 6584-6593 NCBITaxon:7955 denotes zebrafish
T4524 6594-6597 PR_EXT:Q6E2N3 denotes mon
T4525 6598-6605 SO_EXT:sequence_altered_entity denotes mutants
T4526 6614-6629 CL:0000232 denotes red blood cells
T4527 6618-6623 UBERON:0000178 denotes blood
T4528 6624-6629 CL_GO_EXT:cell denotes cells
T4529 6631-6635 CL:0000232 denotes RBCs
T4530 6709-6712 PR_EXT:Q6E2N3 denotes mon
T4531 6713-6720 SO_EXT:sequence_altered_entity denotes mutants
T4532 6730-6740 GO:0010467 denotes expression
T4533 6744-6749 PR_EXT:000007857 denotes gata1
T4534 6753-6766 GO:0030097 denotes hematopoietic
T4535 6753-6772 CL:0000988 denotes hematopoietic cells
T4536 6767-6772 CL_GO_EXT:cell denotes cells
T4537 6789-6795 UBERON:0002329 denotes somite
T4538 6814-6823 SO_EXT:wild_type_entity_or_quality denotes wild-type
T4539 6824-6831 UBERON:0000922 denotes embryos
T4540 6888-6903 GO_RO_EXT:developmental_differentiation_process denotes differentiating
T4541 6904-6919 CL:0000764 denotes erythroid cells
T4542 6914-6919 CL_GO_EXT:cell denotes cells
T4543 6928-6949 GO:0012501 denotes programmed cell death
T4544 6939-6943 CL_GO_EXT:cell denotes cell
T4545 6962-6968 UBERON:0002329 denotes somite
T4546 6987-7000 GO:0009566 denotes fertilization
T4547 7041-7048 UBERON:0002329 denotes somites
T4548 7050-7055 PR_EXT:000007857 denotes gata1
T4549 7056-7066 GO:0010467 denotes expression
T4550 7107-7120 GO:0030097 denotes hematopoietic
T4551 7130-7137 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T4552 7146-7151 PR_EXT:000007857 denotes gata1
T4553 7153-7156 PR_EXT:000016043 denotes scl
T4554 7158-7163 PR_EXT:000007858 denotes gata2
T4555 7169-7175 PR_EXT:000001811 denotes ikaros
T4556 7200-7209 UBERON:0000922 denotes embryonic
T4557 7210-7222 UBERON:0003061 denotes blood island
T4558 7265-7278 GO:0030097 denotes hematopoietic
T4559 7265-7284 CL:0000988 denotes hematopoietic cells
T4560 7279-7284 CL_GO_EXT:cell denotes cells
T4561 7331-7345 _FRAGMENT denotes development of
T4562 7357-7364 GO:0009790 denotes embryos
T4563 7346-7349 PR_EXT:Q6E2N3 denotes mon
T4564 7350-7356 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T4565 7357-7364 UBERON:0000922 denotes embryos
T4566 7395-7399 CL_GO_EXT:cell denotes cell
T4567 7395-7405 GO:0008219 denotes cell death
T4568 7416-7426 GO:0010467 denotes expression
T4569 7430-7435 PR_EXT:000010799 denotes c-myb
T4570 7440-7444 PR_EXT:000003457 denotes rag1
T4571 7466-7469 PR_EXT:Q6E2N3 denotes mon
T4572 7470-7477 SO_EXT:sequence_altered_entity denotes mutants
T4573 7490-7497 CL:0000763 denotes myeloid
T4574 7502-7510 CL_UBERON_EXT:lymphocyte_or_lymphoid_progenitor_or_system_or_tissue denotes lymphoid
T4575 7568-7572 CL:0000232 denotes RBCs
T4576 7576-7579 PR_EXT:Q6E2N3 denotes mon
T4577 7580-7587 SO_EXT:sequence_altered_entity denotes mutants
T4578 7618-7621 UBERON:0008897 denotes fin
T4579 7631-7635 UBERON:0002415 denotes tail
T4580 7636-7646 UBERON:0003104 denotes mesenchyme
T4581 7687-7690 PR_EXT:Q6E2N3 denotes mon
T4582 7691-7698 SO_EXT:sequence_altered_entity denotes mutants
T4583 7722-7752 GO:0097152 denotes apoptosis of mesenchymal cells
T4584 7735-7746 UBERON:0003104 denotes mesenchymal
T4585 7735-7752 CL:0000134 denotes mesenchymal cells
T4586 7747-7752 CL_GO_EXT:cell denotes cells
T4587 7760-7765 UBERON:0002100 denotes trunk
T4588 7770-7778 UBERON:0002533 denotes tail bud
T4589 7819-7822 PR_EXT:Q6E2N3 denotes mon
T4590 7823-7827 SO_EXT:0000704 denotes gene
T4591 7856-7867 _FRAGMENT denotes development
T4592 7881-7883 _FRAGMENT denotes of
T4593 7920-7925 GO:0048468 denotes cells
T4594 7940-7957 GO:0014031 denotes mesenchymal cells
T4595 7899-7925 CL:0000038 denotes erythroid progenitor cells
T4596 7920-7925 CL_GO_EXT:cell denotes cells
T4597 7940-7951 UBERON:0003104 denotes mesenchymal
T4598 7940-7957 CL:0000134 denotes mesenchymal cells
T4599 7952-7957 CL_GO_EXT:cell denotes cells
T4600 8911-8935 GO:0060216 denotes definitive hematopoiesis
T4601 8965-8970 UBERON:0007023 denotes adult
T4602 8971-8980 NCBITaxon:7955 denotes zebrafish
T4603 8981-8984 PR_EXT:Q6E2N3 denotes mon
T4604 8985-8992 SO_EXT:sequence_altered_entity denotes mutants
T4605 8994-9003 SO_EXT:sequence_alteration_entity_or_process denotes Mutations
T4606 9007-9010 PR_EXT:Q6E2N3 denotes mon
T4607 9025-9031 GO_EXT:fatality_or_lethality denotes lethal
T4608 9097-9100 PR_EXT:Q6E2N3 denotes mon
T4609 9112-9119 SO_EXT:sequence_altered_entity denotes mutants
T4610 9144-9149 UBERON:0000178 denotes blood
T4611 9154-9161 UBERON:0000922 denotes embryos
T4612 9177-9184 SO_EXT:0001023 denotes alleles
T4613 9196-9205 UBERON:0000113 denotes adulthood
T4614 9207-9212 UBERON:0007023 denotes Adult
T4615 9213-9216 PR_EXT:Q6E2N3 denotes mon
T4616 9217-9224 SO_EXT:sequence_altered_entity denotes mutants
T4617 9230-9237 UBERON:0000948 denotes cardiac
T4618 9333-9342 SO_EXT:wild_type_entity_or_quality denotes wild-type
T4619 9343-9352 NCBITaxon:7955 denotes zebrafish
T4620 9358-9363 UBERON:0007023 denotes adult
T4621 9372-9385 GO:0030097 denotes hematopoiesis
T4622 9393-9399 UBERON:0002113 denotes kidney
T4623 9442-9451 CL:0000764 denotes erythroid
T4624 9453-9461 CL_UBERON_EXT:lymphocyte_or_lymphoid_progenitor_or_system_or_tissue denotes lymphoid
T4625 9467-9474 CL:0000763 denotes myeloid
T4626 9508-9523 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T4627 9550-9553 PR_EXT:Q6E2N3 denotes mon
T4628 9565-9572 SO_EXT:sequence_altered_entity denotes mutants
T4629 9601-9611 GO_PATO_EXT:biological_maturation denotes maturation
T4630 9619-9634 CL:0000547 denotes proerythroblast
T4631 9665-9683 _FRAGMENT denotes differentiation of
T4632 9692-9697 GO:0030154 denotes cells
T4633 9684-9697 CL:0000763 denotes myeloid cells
T4634 9692-9697 CL_GO_EXT:cell denotes cells
T4635 9755-9758 PR_EXT:Q6E2N3 denotes mon
T4636 9759-9771 SO_EXT:gene_product denotes gene product
T4637 9789-9798 _FRAGMENT denotes primitive
T4638 9814-9828 GO:0060319 denotes erythropoiesis
T4639 9803-9828 GO:0060318 denotes definitive erythropoiesis
T7577 3843-3850 SO_EXT:sequence_cloning_process denotes cloning
T7578 10374-10377 PR_EXT:Q6E2N3 denotes mon
T7579 10385-10394 NCBITaxon:7955 denotes Zebrafish
T7580 10395-10403 SO_EXT:0000855 denotes Ortholog
T7581 10407-10416 NCBITaxon:40674 denotes Mammalian
T7582 10417-10422 PR_EXT:000016655 denotes TIF1γ
T7583 10441-10444 PR_EXT:Q6E2N3 denotes mon
T7584 10445-10449 SO_EXT:0000704 denotes gene
T7585 10464-10471 SO_EXT:sequence_cloning_process denotes cloning
T7586 10503-10510 SO_EXT:sequence_altered_entity denotes mutants
T7587 10590-10593 PR_EXT:Q6E2N3 denotes mon
T7588 10599-10605 SO_EXT:0001023 denotes allele
T7589 10611-10614 PR_EXT:Q6E2N3 denotes mon
T7590 10615-10621 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T7591 10622-10626 SO_EXT:0000704 denotes gene
T7592 10645-10655 GO_SO_EXT:chromosome denotes Chromosome
T7593 10666-10680 SO_EXT:microsatellite_unit_or_region denotes microsatellite
T7594 10681-10688 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T7595 10754-10761 SO_EXT:sequence_cloning_process denotes cloning
T7596 10784-10795 SO_EXT:polymorphism denotes polymorphic
T7597 10796-10803 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T7598 10824-10862 SO_EXT:0001830 denotes amplified fragment length polymorphism
T7599 10864-10868 SO_EXT:0001830 denotes AFLP
T7600 10900-10907 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T7601 10994-10998 SO_EXT:0000704 denotes gene
T7602 11055-11066 GO_SO_EXT:chromosome denotes chromosomal
T7603 11084-11118 SO_EXT:0000154 denotes P1 bacterial artificial chromosome
T7604 11087-11096 NCBITaxon:2 denotes bacterial
T7605 11108-11118 GO_SO_EXT:chromosome denotes chromosome
T7606 11119-11124 SO_EXT:sequence_cloned_entity denotes clone
T7607 11126-11129 SO_EXT:0000154 denotes PAC
T7608 11170-11177 SO_EXT:0000704 denotes genetic
T7609 11199-11207 SO_EXT:biological_sequence denotes sequence
T7610 11221-11233 SO_EXT:polymorphism denotes polymorphism
T7611 11241-11248 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T7612 11263-11266 SO_EXT:0000154 denotes PAC
T7613 11267-11272 SO_EXT:sequence_cloned_entity denotes clone
T7614 11273-11278 SO:0000357 denotes flank
T7615 11292-11299 SO_EXT:0000704 denotes genetic
T7616 11314-11320 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T7617 11339-11351 SO_EXT:sequence_recombinant_entity denotes recombinants
T7618 11365-11372 GO:0007126 denotes meioses
T7619 11373-11382 GO_SO_EXT:telomere_or_telomeric_region_or_telomeric_orientation denotes telomeric
T7620 11390-11398 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T7621 11408-11414 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T7622 11434-11445 SO_EXT:sequence_recombinant_entity denotes recombinant
T7623 11446-11455 GO_SO_EXT:centromere_or_centromeric_region_or_centromeric_orientation denotes centromic
T7624 11463-11471 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T7625 11493-11502 SO_EXT:biological_sequence denotes sequences
T7626 11512-11519 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T7627 11523-11526 SO_EXT:0000154 denotes PAC
T7628 11551-11560 NCBITaxon:7955 denotes zebrafish
T7629 11561-11568 SO_EXT:0001026 denotes genomic
T7630 11569-11577 SO_EXT:biological_sequence denotes sequence
T7631 11578-11584 SO_EXT:0000149 denotes contig
T7632 11645-11654 _FRAGMENT denotes predicted
T7633 11677-11681 SO_EXT:0000996 denotes gene
T7634 11655-11664 NCBITaxon:7955 denotes zebrafish
T7635 11665-11669 PR_EXT:TIF1 denotes TIF1
T7636 11688-11691 SO_EXT:0000154 denotes PAC
T7637 11696-11706 GO:0097617 denotes hybridized
T7638 11712-11718 UBERON:0002113 denotes kidney
T7639 11719-11723 SO_EXT:cDNA denotes cDNA
T7640 11768-11774 SO_EXT:sequence_cloned_entity denotes clones
T7641 11801-11805 SO_EXT:0000704 denotes gene
T7642 13249-13252 PR_EXT:Q6E2N3 denotes mon
T7643 13253-13257 SO_EXT:0000704 denotes gene
T7644 13258-13265 SO_EXT:sequence_coding_function denotes encodes
T7645 13282-13286 PR_EXT:TIF1 denotes TIF1
T7646 13297-13312 GO_EXT:transcription denotes transcriptional
T7647 13297-13322 GO_EXT:0003712 denotes transcriptional cofactors
T7648 13313-13322 CHEBI_EXT:23357 denotes cofactors
T7649 13347-13362 SO_EXT:coding_sequence denotes coding sequence
T7650 13366-13369 PR_EXT:Q6E2N3 denotes mon
T7651 13389-13394 NCBITaxon:9606 denotes human
T7652 13395-13399 PR_EXT:000016655 denotes TIFγ
T7653 13492-13507 SO_EXT:0000333 denotes exon boundaries
T7654 13512-13521 SO_EXT:biological_conservation_process_or_quality denotes conserved
T7655 13534-13543 NCBITaxon:7955 denotes zebrafish
T7656 13548-13553 NCBITaxon:9606 denotes human
T7657 13554-13559 SO_EXT:0000704 denotes genes
T7658 13584-13587 PR_EXT:Q6E2N3 denotes mon
T7659 13588-13596 _FRAGMENT denotes locus on
T7660 13607-13617 GO_SO_EXT:chromosomal_part_or_position_or_region_or_site denotes Chromosome
T7661 13597-13606 NCBITaxon:7955 denotes zebrafish
T7662 13644-13652 SO:0000860 denotes syntenic
T7663 13660-13669 _FRAGMENT denotes region of
T7664 13676-13686 GO_SO_EXT:chromosomal_part_or_position_or_region_or_site denotes Chromosome
T7665 13670-13675 NCBITaxon:9606 denotes human
T7666 13708-13713 PR_EXT:000016655 denotes TIF1γ
T7667 13714-13718 SO_EXT:0000704 denotes gene
T7668 13732-13741 SO_EXT:biological_conservation_process_or_quality denotes conserved
T7669 13764-13775 SO:0000858 denotes orthologous
T7670 13776-13780 SO_EXT:0000704 denotes gene
T7671 13798-13802 PR_EXT:000011416 denotes NRAS
T7672 13831-13836 NCBITaxon:9606 denotes human
T7673 13841-13850 NCBITaxon:7955 denotes zebrafish
T7674 13895-13903 SO_EXT:biological_sequence denotes sequence
T7675 13919-13939 GO_SO_EXT:chromosomal_part_or_position_or_region_or_site denotes chromosomal location
T7676 13945-13954 NCBITaxon:7955 denotes zebrafish
T7677 13955-13958 PR_EXT:Q6E2N3 denotes mon
T7678 13959-13963 SO_EXT:0000704 denotes gene
T7679 13978-13986 SO_EXT:0000855 denotes ortholog
T7680 13994-13999 NCBITaxon:9606 denotes human
T7681 14000-14005 PR_EXT:000016655 denotes TIF1γ
T7682 14006-14010 SO_EXT:0000704 denotes gene
T7683 14031-14048 CHEBI:23995 denotes ethyl-nitrosourea
T7684 14050-14053 CHEBI:23995 denotes ENU
T7685 14063-14078 SO_EXT:point_mutation_entity_or_process denotes point mutations
T7686 14088-14095 SO_EXT:0001023 denotes alleles
T7687 14099-14102 PR_EXT:Q6E2N3 denotes mon
T7688 14149-14169 SO_EXT:0001587 denotes premature stop codon
T7689 14175-14178 PR_EXT:Q6E2N3 denotes mon
T7690 14189-14192 PR_EXT:Q6E2N3 denotes mon
T7691 14198-14205 SO_EXT:0001023 denotes alleles
T7692 14238-14249 _FRAGMENT denotes circulating
T7693 14256-14261 CL:0000080 denotes cells
T7694 14250-14255 UBERON:0000178 denotes blood
T7695 14250-14261 CL:0000081 denotes blood cells
T7696 14256-14261 CL_GO_EXT:cell denotes cells
T7697 14280-14283 PR_EXT:Q6E2N3 denotes mon
T7698 14288-14294 SO_EXT:0001023 denotes allele
T7699 14306-14317 _FRAGMENT denotes circulating
T7700 14324-14329 CL:0000080 denotes cells
T7701 14318-14323 UBERON:0000178 denotes blood
T7702 14318-14329 CL:0000081 denotes blood cells
T7703 14324-14329 CL_GO_EXT:cell denotes cells
T7704 14382-14386 CL:0000232 denotes RBCs
T7705 14409-14418 SO_EXT:wild_type_entity_or_quality denotes wild-type
T7706 14435-14442 UBERON:0000922 denotes embryos
T7707 14471-14474 PR_EXT:Q6E2N3 denotes mon
T7708 14479-14485 SO_EXT:0001023 denotes allele
T7709 14499-14505 SO_EXT:sequence_coding_function denotes encode
T7710 14508-14528 SO_EXT:0001587 denotes premature stop codon
T7711 14558-14564 SO_EXT:sequence_coding_function denotes encode
T7712 14576-14583 CHEBI_PR_EXT:protein denotes protein
T7713 14595-14606 CHEBI_SO_EXT:amino_acid denotes amino acids
T7714 14622-14630 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T7715 14679-14682 PR_EXT:Q6E2N3 denotes mon
T7716 14683-14695 SO_EXT:gene_product denotes gene product
T7717 14705-14715 CHEBI_SO_EXT:methionine denotes methionine
T7718 14725-14735 SO_EXT:sequence_downstreamness denotes downstream
T7719 14739-14749 CHEBI_SO_EXT:amino_acid denotes amino acid
T7720 14773-14784 GO:0006412 denotes translation
T7721 14800-14812 CL:0000558 denotes reticulocyte
T7722 14849-14860 GO:0006412 denotes translation
T7723 14871-14881 CHEBI_SO_EXT:methionine denotes methionine
T7724 14929-14935 UBERON:0002548 denotes larval
T7725 14953-14956 PR_EXT:Q6E2N3 denotes mon
T7726 14961-14967 SO_EXT:0001023 denotes allele
T7727 15001-15004 PR_EXT:Q6E2N3 denotes mon
T7728 15017-15027 GO:0010467 denotes expression
T7729 15045-15054 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T7730 15070-15073 PR_EXT:Q6E2N3 denotes mon
T7731 15074-15081 SO_EXT:0001023 denotes alleles
T7732 15107-15112 PR_EXT:000016655 denotes Tif1γ
T7733 15142-15145 PR_EXT:Q6E2N3 denotes mon
T7734 15187-15192 PR_EXT:000016655 denotes tif1γ
T7735 15196-15205 GO:0010467 denotes expressed
T7736 15209-15222 GO:0030097 denotes hematopoietic
T7737 15223-15231 UBERON:0000926 denotes mesoderm
T7738 15250-15259 NCBITaxon:7955 denotes zebrafish
T7739 15260-15267 UBERON:0000922 denotes embryos
T7740 15291-15304 GO:0097617 denotes hybridization
T7741 15318-15323 PR_EXT:000016655 denotes tif1γ
T7742 15324-15328 CHEBI_SO_EXT:mRNA denotes mRNA
T7743 15332-15341 GO:0010467 denotes expressed
T7744 15381-15387 UBERON:0000922 denotes embryo
T7745 15395-15410 UBERON:0000108 denotes blastula stages
T7746 15419-15431 GO:0007369 denotes gastrulation
T7747 15436-15443 GO:0090504 denotes epiboly
T7748 15452-15459 CL:0000365 denotes zygotic
T7749 15460-15470 GO:0010467 denotes expression
T7750 15474-15477 PR_EXT:Q6E2N3 denotes mon
T7751 15515-15524 UBERON:0002541 denotes germ ring
T7752 15529-15537 UBERON:0002533 denotes tail bud
T7753 15548-15554 UBERON:0002329 denotes somite
T7754 15578-15583 PR_EXT:000016655 denotes tif1γ
T7755 15584-15594 GO:0010467 denotes expression
T7756 15639-15646 _FRAGMENT denotes ventral
T7757 15655-15663 UBERON:2000083 denotes mesoderm
T7758 15647-15663 UBERON:0003081 denotes lateral mesoderm
T7759 15687-15692 UBERON:0000178 denotes blood
T7760 15702-15711 GO:0010467 denotes expresses
T7761 15712-15721 CL:0000034 denotes stem cell
T7762 15712-15764 PR_EXT:000016043 denotes stem cell leukemiahematopoietic transcription factor
T7763 15717-15721 CL_GO_EXT:cell denotes cell
T7764 15730-15743 GO:0030097 denotes hematopoietic
T7765 15744-15757 GO_EXT:transcription denotes transcription
T7766 15744-15764 GO_EXT:transcription_factor denotes transcription factor
T7767 15766-15769 PR_EXT:000016043 denotes scl
T7768 15796-15810 UBERON_EXT:cell_clump_or_cluster_or_group_or_mass_or_population denotes group of cells
T7769 15805-15810 CL_GO_EXT:cell denotes cells
T7770 15824-15831 GO:0010467 denotes express
T7771 15832-15837 PR_EXT:000016655 denotes tif1γ
T7772 15842-15845 PR_EXT:000016043 denotes scl
T7773 15879-15888 UBERON:0000922 denotes embryonic
T7774 15889-15901 UBERON:0003061 denotes blood island
T7775 15929-15934 PR_EXT:000016655 denotes tif1γ
T7776 15935-15939 SO_EXT:0000704 denotes gene
T7777 15955-15964 GO:0010467 denotes expressed
T7778 15972-15994 UBERON:0001017 denotes central nervous system
T7779 16010-16023 _FRAGMENT denotes mesenchyme of
T7780 16028-16033 UBERON:0005256 denotes trunk
T7781 16038-16042 UBERON:0002415 denotes tail
T7782 16055-16058 PR_EXT:Q6E2N3 denotes mon
T7783 16064-16071 SO_EXT:sequence_altered_entity denotes mutants
T7784 16105-16110 PR_EXT:000016655 denotes tif1γ
T7785 16111-16115 CHEBI_SO_EXT:mRNA denotes mRNA
T7786 16123-16130 UBERON:0000479 denotes tissues
T7787 16147-16155 SO_EXT:nonsense_quality_or_entity denotes nonsense
T7788 16147-16178 GO:0000184 denotes nonsense-mediated message decay
T7789 16165-16172 SO_EXT:0000673 denotes message
T7790 16165-16172 CHEBI:33695 denotes message
T7791 16186-16195 NCBITaxon:7955 denotes zebrafish
T7792 16196-16201 PR_EXT:000016655 denotes tif1γ
T7793 16218-16227 GO:0010467 denotes expressed
T7794 16231-16247 UBERON:2000083 denotes ventral mesoderm
T7795 16300-16316 UBERON_EXT:embryonic_stage_or_stage_part denotes embryonic stages
T7796 16322-16335 GO:0030097 denotes hematopoietic
T7797 16363-16372 GO:0006915 denotes apoptosis
T7798 16376-16379 PR_EXT:Q6E2N3 denotes mon
T7799 16380-16387 SO_EXT:sequence_altered_entity denotes mutants
T7800 16410-16420 GO:0010467 denotes expression
T7801 16424-16433 NCBITaxon:7955 denotes zebrafish
T7802 16434-16437 PR_EXT:Q6E2N3 denotes mon
T7803 16441-16446 NCBITaxon:10088 denotes mouse
T7804 16447-16452 PR_EXT:000016655 denotes Tif1γ
T7805 16473-16478 NCBITaxon:10088 denotes Mouse
T7806 16479-16484 PR_EXT:000016655 denotes Tif1γ
T7807 16495-16504 GO:0010467 denotes expressed
T7808 16508-16517 CL:0000764 denotes erythroid
T7809 16518-16534 _FRAGMENT denotes blood islands of
T7815 16729-16738 NCBITaxon:7955 denotes zebrafish
T7816 16739-16742 PR_EXT:Q6E2N3 denotes mon
T7817 16747-16752 NCBITaxon:10088 denotes mouse
T7818 16753-16758 PR_EXT:000016655 denotes Tif1γ
T7819 16763-16772 SO_EXT:0000855 denotes orthologs
T7820 16794-16807 GO:0030097 denotes hematopoiesis
T7821 18133-18142 NCBITaxon:40674 denotes mammalian
T7822 18143-18148 PR_EXT:000016655 denotes TIF1γ
T7823 18179-18188 GO_EXT:oligomeric_macromolecular_complex denotes oligomers
T7824 18194-18199 PR_EXT:000016649 denotes Tif1α
T7825 18247-18251 PR_EXT:TIF1 denotes TIF1
T7826 18270-18279 NCBITaxon:7955 denotes zebrafish
T7827 18296-18301 PR_EXT:000016655 denotes tif1γ
T7828 18309-18318 NCBITaxon:7955 denotes zebrafish
T7829 18319-18328 GO:0010467 denotes expressed
T7830 18319-18341 SO_EXT:0000345 denotes expressed sequence tag
T7831 18338-18341 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes tag
T7832 18343-18346 SO_EXT:0000345 denotes EST
T7833 18348-18357 SO_EXT:biological_sequence denotes sequences
T7834 18371-18378 SO_EXT:0000112 denotes primers
T7835 18382-18384 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T7836 18399-18403 PR_EXT:TIF1 denotes TIF1
T7837 18412-18416 SO_EXT:cDNA denotes cDNA
T7838 18422-18431 UBERON:0000922 denotes embryonic
T7839 18450-18453 CHEBI_SO_EXT:RNA denotes RNA
T7840 18460-18464 SO_EXT:cDNA denotes cDNA
T7841 18465-18472 SO_EXT:sequence_coding_function denotes encodes
T7842 18485-18494 NCBITaxon:7955 denotes zebrafish
T7843 18495-18503 SO_EXT:0000855 denotes ortholog
T7844 18507-18512 NCBITaxon:9606 denotes human
T7845 18513-18518 PR_EXT:000016649 denotes TIF1α
T7846 18538-18548 CHEBI_SO_EXT:amino_acid denotes amino acid
T7847 18549-18558 SO_EXT:biological_sequence denotes sequences
T7848 18589-18598 NCBITaxon:7955 denotes zebrafish
T7849 18599-18604 PR_EXT:000016649 denotes tif1α
T7850 18605-18609 SO_EXT:0000345 denotes ESTs
T7851 18649-18657 SO:0000860 denotes syntenic
T7852 18665-18674 _FRAGMENT denotes region of
T7853 18681-18691 GO_SO_EXT:chromosomal_part_or_position_or_region_or_site denotes Chromosome
T7854 18675-18680 NCBITaxon:9606 denotes human
T7855 18712-18717 PR_EXT:000016649 denotes TIF1α
T7856 18718-18722 SO_EXT:0000704 denotes gene
T7857 18736-18745 SO_EXT:biological_conservation_process_or_quality denotes conserved
T7858 18771-18782 SO:0000858 denotes orthologous
T7859 18783-18787 SO_EXT:0000704 denotes gene
T7860 18805-18811 PR_EXT:000014615 denotes SEMA3A
T7861 18840-18845 NCBITaxon:9606 denotes human
T7862 18850-18859 NCBITaxon:7955 denotes zebrafish
T7863 18905-18914 UBERON:0000922 denotes embryonic
T7864 18915-18925 GO:0010467 denotes expression
T7865 18937-18942 PR_EXT:000016649 denotes tif1α
T7866 18943-18947 CHEBI_SO_EXT:mRNA denotes mRNA
T7867 18951-18956 PR_EXT:000016655 denotes tif1γ
T7868 18968-18981 GO:0097617 denotes hybridization
T7869 18988-18997 NCBITaxon:40674 denotes mammalian
T7870 18998-19003 PR_EXT:000016649 denotes TIF1α
T7871 19061-19070 _FRAGMENT denotes predicted
T7872 19087-19091 SO_EXT:0000996 denotes gene
T7873 19071-19080 NCBITaxon:7955 denotes zebrafish
T7874 19081-19086 PR_EXT:000016655 denotes tif1γ
T7875 19103-19112 GO:0010467 denotes expressed
T7876 19138-19145 UBERON:0002329 denotes somites
T7877 19147-19156 NCBITaxon:7955 denotes zebrafish
T7878 19157-19162 PR_EXT:000016649 denotes tif1α
T7879 19200-19210 GO:0010467 denotes expression
T7880 19245-19258 GO:0030097 denotes hematopoietic
T7881 19259-19267 UBERON:0000926 denotes mesoderm
T7882 19278-19283 PR_EXT:000016655 denotes tif1γ
T7883 19302-19307 PR_EXT:000016649 denotes tif1α
T7884 19318-19327 GO:0010467 denotes expressed
T7885 19351-19357 UBERON:0000922 denotes embryo
T7886 19384-19397 UBERON:0003061 denotes blood islands
T7887 19410-19415 PR_EXT:000016649 denotes tif1α
T7888 19421-19430 GO:0010467 denotes expressed
T7889 19443-19448 CL_GO_EXT:cell denotes cells
T7890 19454-19459 PR_EXT:000016655 denotes tif1γ
T7891 19506-19515 GO_EXT:oligomeric_macromolecular_complex denotes oligomers
T17008 35205-35210 NCBITaxon:10088 denotes mouse
T9602 20304-20321 CL:0000134 denotes mesenchymal cells
T9603 20316-20321 CL_GO_EXT:cell denotes cells
T9562 19533-19543 GO:0010467 denotes Expression
T9563 19547-19552 PR_EXT:000016655 denotes tif1γ
T9564 19553-19560 SO_EXT:sequence_rescue_process denotes Rescues
T9565 19561-19574 GO:0030097 denotes Hematopoiesis
T9566 19578-19581 PR_EXT:Q6E2N3 denotes mon
T9567 19582-19589 SO_EXT:sequence_altered_entity denotes Mutants
T9568 19616-19624 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T9569 19632-19641 NCBITaxon:7955 denotes zebrafish
T9570 19642-19647 PR_EXT:000016655 denotes tif1γ
T9571 19648-19652 SO_EXT:0000704 denotes gene
T9572 19676-19679 PR_EXT:Q6E2N3 denotes mon
T9573 19680-19686 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T9574 19710-19716 UBERON:0000922 denotes embryo
T9575 19717-19723 SO_EXT:sequence_rescue_process denotes rescue
T9576 19786-19795 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9577 19796-19799 PR_EXT:Q6E2N3 denotes mon
T9578 19800-19804 CHEBI_SO_EXT:mRNA denotes mRNA
T9579 19816-19820 CL_GO_EXT:cell denotes cell
T9580 19827-19834 SO_EXT:sequence_rescue_process denotes rescues
T9581 19839-19851 _FRAGMENT denotes formation of
T9582 19862-19874 GO:0048821 denotes erythrocytes
T9583 19852-19861 UBERON:0000922 denotes embryonic
T9584 19862-19874 CL:0000232 denotes erythrocytes
T9585 19888-19894 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T9586 19895-19902 UBERON:0000922 denotes embryos
T9587 19938-19947 UBERON:0000922 denotes embryonic
T9588 19938-19947 _FRAGMENT denotes embryonic
T9589 19962-19975 GO:0048568 denotes organogenesis
T9590 19948-19958 GO_PATO_EXT:biological_patterning_process_or_quality denotes patterning
T9591 20016-20019 PR_EXT:Q6E2N3 denotes mon
T9592 20025-20032 SO_EXT:sequence_altered_entity denotes mutants
T9593 20068-20073 CL_GO_EXT:cell denotes cells
T9594 20093-20102 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9595 20121-20126 CL_GO_EXT:cell denotes cells
T9596 20128-20134 SO_EXT:sequence_rescue_process denotes rescue
T9597 20150-20164 GO_EXT:hemoglobin_biosynthetic_process_or_import denotes hemoglobinized
T9598 20165-20169 CL:0000232 denotes RBCs
T9599 20203-20210 SO_EXT:sequence_altered_entity denotes mutants
T9600 20259-20262 UBERON:0008897 denotes fin
T9601 20304-20315 UBERON:0003104 denotes mesenchymal
T9604 20326-20333 SO_EXT:sequence_rescue_process denotes rescued
T9605 20392-20402 GO:0010467 denotes expression
T9606 20406-20409 PR_EXT:Q6E2N3 denotes mon
T9607 20437-20442 UBERON:0000178 denotes blood
T9608 20437-20447 CL:0000081 denotes blood cell
T9609 20443-20447 CL_GO_EXT:cell denotes cell
T9610 20459-20468 SO_EXT:wild_type_entity_or_quality denotes wild-type
T9611 20469-20476 UBERON:0000922 denotes embryos
T9612 20509-20515 SO_EXT:sequence_rescue_process denotes rescue
T9613 20533-20536 PR_EXT:Q6E2N3 denotes mon
T9614 20537-20544 SO_EXT:sequence_altered_entity denotes mutants
T9615 20605-20610 UBERON:0000178 denotes blood
T9616 20630-20637 UBERON:0000922 denotes embryos
T9617 20645-20651 SO_EXT:sequence_rescue_process denotes rescue
T9618 20677-20680 PR_EXT:Q6E2N3 denotes mon
T9619 20727-20740 GO:0030097 denotes hematopoiesis
T17009 35231-35240 NCBITaxon:7955 denotes Zebrafish
T17010 35305-35312 SO_EXT:0001023 denotes alleles
T10579 22465-22472 SO_EXT:sequence_rescue_process denotes Rescues
T10580 22473-22487 GO:0030218 denotes Erythropoiesis
T10581 22491-22494 PR_EXT:Q6E2N3 denotes mon
T10582 22495-22502 SO_EXT:sequence_altered_entity denotes Mutants
T10583 22522-22527 PR_EXT:000016655 denotes tif1γ
T10584 22528-22538 GO:0010467 denotes expression
T10585 22542-22557 CL:0000764 denotes erythroid cells
T10586 22552-22557 CL_GO_EXT:cell denotes cells
T10587 22589-22593 CL_GO_EXT:cell denotes cell
T10588 22605-22614 GO_EXT:regulator denotes regulator
T10589 22618-22622 SO_EXT:0000704 denotes gene
T10590 22618-22633 GO:0010467 denotes gene expression
T10591 22637-22650 GO:0030097 denotes hematopoietic
T10592 22637-22656 CL:0000988 denotes hematopoietic cells
T10593 22651-22656 CL_GO_EXT:cell denotes cells
T10594 22708-22717 SO_EXT:wild_type_entity_or_quality denotes wild-type
T10595 22718-22723 UBERON:0007023 denotes adult
T10596 22724-22733 NCBITaxon:7955 denotes zebrafish
T10597 22734-22747 UBERON:0007132 denotes kidney marrow
T10598 22748-22753 CL_GO_EXT:cell denotes cells
T10599 22765-22770 PR_EXT:000007857 denotes gata1
T10600 22789-22796 CHEBI_PR_EXT:protein denotes protein
T10601 22803-22812 SO_EXT:0000902 denotes transgene
T10602 22825-22828 PR_EXT:Q6E2N3 denotes mon
T10603 22829-22835 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T10604 22836-22843 UBERON:0000922 denotes embryos
T10605 22870-22875 PR_EXT:000007857 denotes gata1
T10606 22880-22889 SO_EXT:0000902 denotes transgene
T10607 22907-22912 PR_EXT:000007857 denotes gata1
T10608 22913-22921 SO_EXT:0000167 denotes promoter
T10609 22935-22945 GO:0010467 denotes expression
T10610 22970-22974 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes mark
T10611 22989-23004 CL:0000764 denotes erythroid cells
T10612 22999-23004 CL_GO_EXT:cell denotes cells
T10613 23040-23046 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T10614 23047-23054 UBERON:0000922 denotes embryos
T10615 23063-23072 UBERON:0000922 denotes embryonic
T10616 23063-23072 _FRAGMENT denotes embryonic
T10617 23079-23084 CL:0002321 denotes cells
T10618 23073-23078 UBERON:0000178 denotes blood
T10619 23073-23084 CL:0000081 denotes blood cells
T10620 23079-23084 CL_GO_EXT:cell denotes cells
T10621 23079-23099 CL:0000080 denotes cells in circulation
T10622 23128-23134 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T10623 23135-23139 GO:0018995 denotes host
T10624 23140-23147 UBERON:0000922 denotes embryos
T10625 23185-23191 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T10626 23192-23199 UBERON:0000922 denotes embryos
T10627 23232-23237 CL_GO_EXT:cell denotes cells
T10628 23232-23252 CL:0000080 denotes cells in circulation
T10629 23321-23326 CL_GO_EXT:cell denotes cells
T10630 23419-23424 CL_GO_EXT:cell denotes cells
T10631 23502-23517 CL:0000764 denotes erythroid cells
T10632 23512-23517 CL_GO_EXT:cell denotes cells
T10633 23538-23543 CL_GO_EXT:cell denotes cells
T10634 23538-23558 CL:0000080 denotes cells in circulation
T10635 23600-23607 UBERON:0000922 denotes embryos
T10636 23635-23640 CL_GO_EXT:cell denotes cells
T10637 23635-23655 CL:0000080 denotes cells in circulation
T10638 23682-23687 UBERON:0000178 denotes blood
T10639 23682-23693 CL:0000081 denotes blood cells
T10640 23688-23693 CL_GO_EXT:cell denotes cells
T10641 23704-23711 UBERON:0000922 denotes embryos
T10642 23746-23751 UBERON:0000178 denotes blood
T10643 23746-23757 CL:0000081 denotes blood cells
T10644 23752-23757 CL_GO_EXT:cell denotes cells
T10645 23759-23765 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T10646 23799-23803 GO:0036268 denotes swim
T10647 23799-23812 UBERON:0006860 denotes swim bladders
T10648 23890-23895 GO:0016265 denotes dying
T10649 23950-23953 PR_EXT:Q6E2N3 denotes mon
T10650 23989-23998 UBERON:0000113 denotes adulthood
T10651 24023-24032 SO_EXT:wild_type_entity_or_quality denotes wild-type
T10652 24033-24038 CL_GO_EXT:cell denotes cells
T10653 24049-24055 SO_EXT:sequence_rescue_process denotes rescue
T10654 24068-24073 PR_EXT:000007857 denotes gata1
T10655 24075-24082 SO_EXT:sequence_altered_entity denotes mutants
T10656 24131-24135 CL_GO_EXT:cell denotes cell
T10657 24167-24172 PR_EXT:000016655 denotes tif1γ
T10658 24181-24185 CL_GO_EXT:cell denotes cell
T10659 24205-24220 CL:0000764 denotes erythroid cells
T10660 24215-24220 CL_GO_EXT:cell denotes cells
T10661 24241-24254 GO:0030097 denotes hematopoietic
T10662 24255-24262 UBERON:0000479 denotes tissues
T10663 24272-24288 UBERON:0005256 denotes trunk mesenchyme
T10664 24296-24310 UBERON:0001016 denotes nervous system
T10665 24333-24339 UBERON:0000922 denotes embryo
T17011 35313-35316 PR_EXT:Q6E2N3 denotes mon
T17012 35327-35330 PR_EXT:Q6E2N3 denotes mon
T11853 24924-24929 PR_EXT:000016655 denotes Tif1γ
T11854 24942-24949 GO:0005634 denotes Nuclear
T11855 24974-24983 GO:0065007 denotes Regulated
T11856 25008-25019 GO_UBERON_EXT:cellular_component_or_cell_part denotes subcellular
T11857 25011-25019 CL_GO_EXT:cell denotes cellular
T11858 25036-25041 PR_EXT:000016655 denotes Tif1γ
T11859 25042-25049 CHEBI_PR_EXT:protein denotes protein
T11860 25085-25091 NCBITaxon:9986 denotes rabbit
T11861 25107-25112 UBERON:0001977 denotes serum
T11862 25113-25121 GO_EXT:biological_direction_or_guidance denotes directed
T11863 25134-25144 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T11864 25148-25159 CHEBI_SO_EXT:amino_acid denotes amino acids
T11865 25160-25169 SO_EXT:biological_conservation_process_or_quality denotes conserved
T11866 25173-25178 NCBITaxon:9606 denotes human
T11867 25179-25184 PR_EXT:000016655 denotes TIF1γ
T11868 25189-25194 NCBITaxon:10088 denotes mouse
T11869 25195-25200 PR_EXT:000016655 denotes Tif1γ
T11870 25224-25229 NCBITaxon:10088 denotes mouse
T11871 25230-25236 UBERON:0000922 denotes embryo
T11872 25237-25247 CL:0000057 denotes fibroblast
T11873 25248-25254 GO:0005634 denotes nuclei
T11874 25269-25274 PR_EXT:000016655 denotes Tif1γ
T11875 25279-25284 UBERON:0001977 denotes serum
T11876 25303-25308 PR_EXT:000016655 denotes Tif1γ
T11877 25312-25321 GO_PATO_EXT:biological_localization_process_or_quality denotes localized
T11878 25331-25338 GO:0005634 denotes nuclear
T11879 25361-25373 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T11880 25377-25382 PR_EXT:000016655 denotes Tif1γ
T11881 25383-25390 CHEBI_PR_EXT:protein denotes protein
T11882 25431-25438 GO:0005634 denotes nuclear
T11883 25477-25482 PR_EXT:000016649 denotes Tif1α
T11884 25512-25517 PR_EXT:000016652 denotes TIF1β
T11885 25574-25579 PR_EXT:000016652 denotes TIF1β
T11886 25596-25611 GO:0000792 denotes heterochromatin
T11887 25634-25647 CHEBI:26536 denotes retinoic acid
T11888 25661-25666 UBERON:0001977 denotes serum
T11889 25706-25718 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T11890 25722-25732 GO:0010467 denotes expression
T11891 25740-25744 PR_EXT:TIF1 denotes TIF1
T11892 25745-25753 CHEBI_PR_EXT:protein denotes proteins
T11893 25761-25770 GO:0065007 denotes regulated
T11894 25841-25848 GO:0005634 denotes nuclear
T11895 25867-25872 PR_EXT:000016655 denotes Tif1γ
T11896 25882-25890 GO_PATO_EXT:biological_localization_process_or_quality denotes localize
T11897 25900-25907 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T11898 25911-25926 GO:0000792 denotes heterochromatin
T11899 25928-25932 PR_EXT:000005086 denotes HP1α
T11900 25933-25940 CHEBI_PR_EXT:protein denotes protein
T11901 25945-25949 CHEBI:51231 denotes DAPI
T11902 25962-25965 CHEBI_SO_EXT:DNA denotes DNA
T11903 25992-25997 PR_EXT:000016655 denotes Tif1γ
T11904 26009-26017 GO_PATO_EXT:biological_localization_process_or_quality denotes localize
T11905 26023-26036 CL:0000836 denotes promyelocytic
T11906 26023-26045 PR_EXT:000026474 denotes promyelocytic leukemia
T11907 26023-26058 _FRAGMENT denotes promyelocytic leukemia gene product
T11908 26065-26079 GO:0016605 denotes nuclear bodies
T11909 26046-26058 SO_EXT:gene_product denotes gene product
T11910 26060-26063 PR_EXT:000026474 denotes PML
T11911 26060-26063 _FRAGMENT denotes PML
T11912 26081-26084 CHEBI_SO_EXT:DNA denotes DNA
T11913 26081-26091 GO:0006281 denotes DNA repair
T11914 26081-26101 GO:1990391 denotes DNA repair complexes
T11915 26115-26120 PR_EXT:000010562 denotes Mre11
T11916 26125-26140 GO_EXT:transcription denotes transcriptional
T11917 26141-26150 GO:0032991 denotes complexes
T11918 26162-26168 PR_EXT:000008313 denotes TFII-B
T11919 26210-26220 GO:0010467 denotes expression
T11920 26224-26229 PR_EXT:000016655 denotes Tif1γ
T11921 26230-26237 CHEBI_PR_EXT:protein denotes protein
T11922 26249-26267 _FRAGMENT denotes differentiation of
T11923 26272-26277 GO:0030154 denotes cells
T11924 26272-26277 CL_GO_EXT:cell denotes cells
T11925 26281-26287 NCBITaxon:39107 denotes murine
T11926 26304-26308 CL_GO_EXT:cell denotes cell
T11927 26334-26347 GO_RO_EXT:developmental_differentiation_process denotes differentiate
T11928 26353-26365 CL:0000232 denotes erythrocytes
T11929 26373-26378 PR_EXT:000007857 denotes Gata1
T11930 26379-26387 CHEBI_EXT:50114 denotes estrogen
T11931 26379-26396 GO_PR_EXT:estrogen_receptor denotes estrogen receptor
T11932 26397-26403 SO_EXT:sequence_fusion_entity_or_process denotes fusion
T11933 26404-26411 CHEBI_PR_EXT:protein denotes protein
T11934 26429-26437 GO_EXT:reaction_or_response denotes response
T11935 26441-26449 CHEBI_EXT:50114 denotes estrogen
T11936 26520-26525 PR_EXT:000016655 denotes Tif1γ
T11937 26526-26533 CHEBI_PR_EXT:protein denotes protein
T11938 26534-26544 GO:0010467 denotes expression
T11939 26569-26578 CL:0000764 denotes erythroid
T11940 26579-26594 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T11941 26652-26661 NCBITaxon:7955 denotes zebrafish
T11942 26662-26665 PR_EXT:Q6E2N3 denotes mon
T11943 26666-26670 CHEBI_SO_EXT:mRNA denotes mRNA
T11944 26671-26681 GO:0010467 denotes expression
T11945 26708-26721 _FRAGMENT denotes maturation of
T11946 26746-26751 GO:0048469 denotes cells
T11947 26736-26751 CL:0000764 denotes erythroid cells
T11948 26746-26751 CL_GO_EXT:cell denotes cells
T11949 26789-26795 NCBITaxon:39107 denotes murine
T11950 26812-26816 CL_GO_EXT:cell denotes cell
T11951 26838-26843 PR_EXT:000016655 denotes Tif1γ
T11952 26852-26861 GO:0010467 denotes expressed
T11953 26865-26872 GO:0005634 denotes nuclear
T11954 26907-26912 PR_EXT:000016655 denotes Tif1γ
T11955 26913-26920 CHEBI_PR_EXT:protein denotes protein
T11956 26944-26951 GO:0005634 denotes nuclear
T11957 26957-26969 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T11958 26991-27006 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T11959 27081-27086 PR_EXT:000016655 denotes Tif1γ
T11960 27105-27112 GO:0005634 denotes nuclear
T11961 27126-27135 CL:0000764 denotes erythroid
T11962 27136-27151 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T17013 35379-35382 CHEBI:23995 denotes ENU
T17016 35453-35459 SO_EXT:0001023 denotes allele
T12524 4-13 NCBITaxon:7955 denotes Zebrafish
T12525 27955-27964 CHEBI_EXT:polyatomic_entity_or_group denotes molecular
T12526 27975-27986 GO:0065007 denotes controlling
T12527 27987-27991 SO_EXT:0000704 denotes gene
T12528 27987-28002 GO:0010467 denotes gene expression
T12529 28010-28023 GO:0030097 denotes hematopoiesis
T12530 28103-28110 SO_EXT:0000704 denotes genetic
T12531 28183-28189 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T12532 28190-28197 SO_EXT:0001023 denotes alleles
T12533 28205-28214 NCBITaxon:7955 denotes zebrafish
T12534 28215-28219 SO_EXT:0000704 denotes gene
T12535 28234-28243 PR_EXT:Q6E2N3 denotes moonshine
T12536 28277-28284 SO_EXT:sequence_cloning_process denotes cloning
T12537 28310-28313 PR_EXT:Q6E2N3 denotes mon
T12538 28314-28318 SO_EXT:0000704 denotes gene
T12539 28355-28370 GO_EXT:transcription denotes transcriptional
T12540 28392-28397 PR_EXT:000016655 denotes Tif1γ
T12541 28406-28419 GO:0030097 denotes hematopoietic
T13468 28438-28441 PR_EXT:Q6E2N3 denotes mon
T13469 28442-28446 SO_EXT:0000704 denotes Gene
T13470 28447-28454 SO_EXT:sequence_coding_function denotes Encodes
T13471 28459-28468 NCBITaxon:7955 denotes Zebrafish
T13472 28469-28477 SO_EXT:0000855 denotes Ortholog
T13473 28481-28490 NCBITaxon:40674 denotes Mammalian
T13474 28491-28496 PR_EXT:000016655 denotes TIF1γ
T13475 28567-28573 SO_EXT:sequence_cloning_process denotes cloned
T13476 28578-28587 NCBITaxon:7955 denotes zebrafish
T13477 28588-28591 PR_EXT:Q6E2N3 denotes mon
T13478 28592-28596 SO_EXT:0000704 denotes gene
T13479 28622-28630 SO_EXT:0000855 denotes ortholog
T13480 28634-28643 NCBITaxon:40674 denotes mammalian
T13481 28644-28649 PR_EXT:000016655 denotes Tif1γ
T13482 28651-28656 PR_EXT:000016655 denotes Tif1γ
T13483 28684-28691 SO_EXT:0000704 denotes genetic
T13484 28741-28742 CHEBI_SO_EXT:base denotes b
T13485 28743-28746 SO_EXT:0000154 denotes PAC
T13486 28747-28752 SO_EXT:sequence_cloned_entity denotes clone
T13487 28797-28805 SO_EXT:biological_sequence denotes Sequence
T13488 28830-28839 NCBITaxon:7955 denotes zebrafish
T13489 28840-28845 PR_EXT:000016655 denotes tif1γ
T13490 28875-28885 CHEBI_SO_EXT:amino_acid denotes amino acid
T13491 28886-28894 SO_EXT:biological_sequence denotes sequence
T13492 28899-28905 SO_EXT:0000188 denotes intron
T13493 28906-28910 SO_EXT:0000147 denotes exon
T13494 28937-28946 _FRAGMENT denotes predicted
T13495 28975-28980 SO_EXT:0000996 denotes genes
T13496 28947-28958 SO:0000858 denotes orthologous
T13497 28959-28964 NCBITaxon:9606 denotes human
T13498 28969-28974 NCBITaxon:10088 denotes mouse
T13499 28982-28991 NCBITaxon:7955 denotes Zebrafish
T13500 28992-28997 PR_EXT:000016655 denotes tif1γ
T13501 29014-29023 _FRAGMENT denotes region of
T13502 29034-29044 GO_SO_EXT:chromosomal_part_or_position_or_region_or_site denotes Chromosome
T13503 29024-29033 NCBITaxon:7955 denotes zebrafish
T13504 29047-29055 SO:0000860 denotes syntenic
T13505 29063-29072 _FRAGMENT denotes region of
T13506 29079-29089 GO_SO_EXT:chromosomal_part_or_position_or_region_or_site denotes Chromosome
T13507 29073-29078 NCBITaxon:9606 denotes human
T13508 29103-29108 PR_EXT:000016655 denotes TIF1γ
T13509 29124-29139 SO_EXT:point_mutation_entity_or_process denotes point mutations
T13510 29143-29148 PR_EXT:000016655 denotes tif1γ
T13511 29170-29177 SO_EXT:0001023 denotes alleles
T13512 29181-29184 PR_EXT:Q6E2N3 denotes mon
T13513 29205-29226 SO_EXT:0001587 denotes premature stop codons
T13514 29231-29235 CHEBI_SO_EXT:mRNA denotes mRNA
T13515 29231-29241 GO:0006402 denotes mRNA decay
T13516 29256-29261 PR_EXT:000016655 denotes tif1γ
T13517 29262-29267 PR_EXT:000016655 denotes Tif1γ
T13518 29278-29287 GO:0010467 denotes expressed
T13519 29291-29304 GO:0030097 denotes hematopoietic
T13520 29291-29310 CL:0000988 denotes hematopoietic cells
T13521 29305-29310 CL_GO_EXT:cell denotes cells
T13522 29322-29335 GO:0009790 denotes embryogenesis
T13523 29344-29353 NCBITaxon:7955 denotes zebrafish
T13524 29358-29363 NCBITaxon:10088 denotes mouse
T13525 29405-29415 GO:0010467 denotes expression
T13526 29419-29428 SO_EXT:wild_type_entity_or_quality denotes wild-type
T13527 29429-29434 PR_EXT:000016655 denotes tif1γ
T13528 29435-29439 CHEBI_SO_EXT:mRNA denotes mRNA
T13529 29452-29459 SO_EXT:sequence_rescue_process denotes rescues
T13530 29460-29473 GO:0030097 denotes hematopoiesis
T13531 29477-29480 PR_EXT:Q6E2N3 denotes mon
T13532 29481-29488 SO_EXT:sequence_altered_entity denotes mutants
T13533 29510-29526 _FRAGMENT denotes hematopoiesis in
T13534 29537-29544 GO:0035162 denotes embryos
T13535 29527-29536 SO_EXT:wild_type_entity_or_quality denotes wild-type
T13536 29537-29544 UBERON:0000922 denotes embryos
T13537 29574-29580 SO_EXT:sequence_cloning_process denotes cloned
T13538 29595-29604 NCBITaxon:7955 denotes zebrafish
T13539 29605-29613 SO_EXT:0000855 denotes ortholog
T13540 29617-29622 PR_EXT:000016649 denotes tif1α
T13541 29648-29657 GO:0010467 denotes expressed
T13542 29661-29670 NCBITaxon:7955 denotes zebrafish
T13543 29671-29678 UBERON:0000922 denotes embryos
T13544 29684-29693 NCBITaxon:40674 denotes mammalian
T13545 29694-29699 PR_EXT:000016649 denotes TIF1α
T13546 29822-29831 GO_EXT:oligomeric_macromolecular_complex denotes oligomers
T13547 29837-29842 PR_EXT:000016655 denotes Tif1γ
T13548 29843-29850 CHEBI_PR_EXT:protein denotes protein
T13549 29865-29878 GO:0030097 denotes hematopoietic
T13550 29865-29884 CL:0000988 denotes hematopoietic cells
T13551 29879-29884 CL_GO_EXT:cell denotes cells
T13552 29906-29915 NCBITaxon:7955 denotes zebrafish
T13553 29920-29929 NCBITaxon:40674 denotes mammalian
T13554 29930-29934 PR_EXT:TIF1 denotes TIF1
T13555 29945-29955 CHEBI_SO_EXT:amino_acid denotes amino acid
T13556 29956-29965 SO_EXT:biological_sequence denotes sequences
T13557 29987-29992 PR_EXT:000016655 denotes Tif1γ
T13558 29993-30002 SO_EXT:0000855 denotes orthologs
T13559 30023-30032 SO_EXT:biological_conservation_process_or_quality denotes conserved
T13560 30058-30063 PR_EXT:000016649 denotes Tif1α
T13561 30064-30073 SO_EXT:biological_sequence denotes sequences
T13562 30125-30130 PR_EXT:000016652 denotes Tif1β
T13563 30131-30139 SO_EXT:0000855 denotes ortholog
T13564 30158-30167 NCBITaxon:7955 denotes zebrafish
T13565 30171-30175 NCBITaxon:31032 denotes fugu
T13566 30176-30182 SO_EXT:0001026 denotes genome
T13567 30186-30189 SO_EXT:0000345 denotes EST
T13568 30190-30199 SO_EXT:biological_sequence denotes sequences
T13569 30221-30226 PR_EXT:000016652 denotes Tif1β
T13570 30242-30248 SO_EXT:0000417 denotes domain
T13571 30249-30262 GO_EXT:transcription denotes transcription
T13572 30249-30270 GO_EXT:transcription_factor denotes transcription factors
T13573 30274-30279 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binds
T13574 30307-30316 NCBITaxon:32523 denotes tetrapods
T13575 30356-30362 SO_EXT:0001026 denotes genome
T13576 30363-30372 SO_EXT:biological_sequence denotes sequences
T13577 30441-30450 NCBITaxon:7955 denotes zebrafish
T13578 30451-30454 PR_EXT:Q6E2N3 denotes mon
T13579 30455-30462 SO_EXT:sequence_altered_entity denotes mutants
T13580 30497-30502 PR_EXT:000016655 denotes Tif1γ
T13581 30513-30537 SO_EXT:biological_conservation_process_or_quality denotes evolutionarily conserved
T13582 30538-30542 PR_EXT:TIF1 denotes TIF1
T13583 30594-30599 NCBITaxon:9606 denotes human
T13584 30604-30609 NCBITaxon:10088 denotes mouse
T13585 30610-30623 GO:0030097 denotes hematopoiesis
T17017 35584-35590 GO:0007618 denotes mating
T15088 30626-30635 SO_EXT:sequence_alteration_entity_or_process denotes Mutations
T15089 30639-30644 PR_EXT:000016655 denotes tif1γ
T15090 30651-30660 GO:0006915 denotes Apoptosis
T15091 30664-30673 CL:0000764 denotes Erythroid
T15092 30705-30718 GO:0030097 denotes hematopoietic
T15093 30719-30723 SO_EXT:0000704 denotes gene
T15094 30719-30734 GO:0010467 denotes gene expression
T15095 30736-30745 GO:0006915 denotes apoptosis
T15096 30771-30774 PR_EXT:Q6E2N3 denotes mon
T15097 30775-30782 SO_EXT:sequence_altered_entity denotes mutants
T15098 30807-30816 CL:0000764 denotes erythroid
T15099 30854-30861 SO_EXT:sequence_altered_entity denotes mutants
T15100 30889-30902 GO_RO_EXT:developmental_differentiation_process denotes differentiate
T15101 30923-30944 GO:0012501 denotes programmed cell death
T15102 30934-30938 CL_GO_EXT:cell denotes cell
T15103 30965-30975 GO:0010467 denotes expression
T15104 30979-30984 PR_EXT:000007857 denotes gata1
T15105 31031-31046 CL:0000764 denotes erythroid cells
T15106 31041-31046 CL_GO_EXT:cell denotes cells
T15107 31050-31053 PR_EXT:Q6E2N3 denotes mon
T15108 31054-31061 SO_EXT:sequence_altered_entity denotes mutants
T15109 31076-31081 CL_GO_EXT:cell denotes cells
T15110 31125-31130 PR_EXT:000007857 denotes gata1
T15111 31131-31141 GO:0010467 denotes expression
T15112 31158-31167 GO_PATO_EXT:apoptotic_process_or_quality denotes apoptotic
T15113 31158-31173 CL:0000445 denotes apoptotic cells
T15114 31168-31173 CL_GO_EXT:cell denotes cells
T15115 31197-31203 UBERON:0002329 denotes somite
T15116 31244-31257 GO:0030097 denotes hematopoietic
T15117 31258-31262 SO_EXT:0000704 denotes gene
T15118 31258-31273 GO:0010467 denotes gene expression
T15119 31295-31305 GO:0010467 denotes expression
T15120 31309-31315 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T15121 31316-31321 SO_EXT:0000704 denotes genes
T15122 31333-31336 PR_EXT:000016043 denotes scl
T15123 31341-31346 PR_EXT:000007858 denotes gata2
T15124 31366-31379 GO:0030097 denotes hematopoietic
T15125 31366-31390 CL:0000037 denotes hematopoietic stem cells
T15126 31385-31390 CL_GO_EXT:cell denotes cells
T15127 31395-31418 GO:0060215 denotes primitive hematopoietic
T15128 31461-31470 UBERON:0000922 denotes embryonic
T15129 31471-31484 UBERON:0003061 denotes blood islands
T15130 31488-31495 SO_EXT:sequence_altered_entity denotes mutants
T15131 31531-31537 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T15132 31538-31551 GO:0030097 denotes hematopoietic
T15133 31538-31557 CL:0000988 denotes hematopoietic cells
T15134 31552-31557 CL_GO_EXT:cell denotes cells
T15135 31601-31610 CL:0000764 denotes erythroid
T15136 31652-31667 CL:0000764 denotes erythroid cells
T15137 31662-31667 CL_GO_EXT:cell denotes cells
T15138 31680-31689 GO:0006915 denotes apoptosis
T15139 31702-31707 PR_EXT:000007857 denotes gata1
T15140 31718-31733 CL:0000764 denotes erythroid cells
T15141 31728-31733 CL_GO_EXT:cell denotes cells
T15142 31787-31801 GO:0030218 denotes erythropoiesis
T15143 31868-31874 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T15144 31875-31878 PR_EXT:Q6E2N3 denotes mon
T15145 31879-31885 UBERON:0007023 denotes adults
T15146 31906-31911 PR_EXT:000016655 denotes tif1γ
T15147 31932-31956 GO:0060216 denotes definitive hematopoiesis
T15148 31977-31984 CL:0000365 denotes zygotic
T15149 31999-32002 PR_EXT:Q6E2N3 denotes mon
T15150 32003-32010 SO_EXT:sequence_altered_entity denotes mutants
T15151 32063-32068 PR_EXT:000016655 denotes Tif1γ
T15152 32081-32090 GO:0010467 denotes expressed
T15153 32091-32100 NCBITaxon:7955 denotes zebrafish
T15154 32101-32106 PR_EXT:000016655 denotes Tif1γ
T15155 32125-32138 GO:0030097 denotes hematopoiesis
T15156 32159-32172 GO:0048513 denotes organogenesis
T15157 32220-32229 SO_EXT:wild_type_entity_or_quality denotes wild-type
T15158 32230-32234 CHEBI_SO_EXT:mRNA denotes mRNA
T15159 32238-32242 CL:0000025 denotes eggs
T15160 32313-32316 PR_EXT:Q6E2N3 denotes mon
T15161 32317-32323 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T15162 32324-32330 PATO_UBERON_EXT:female_or_bearer_of_femaleness denotes female
T15163 32331-32340 NCBITaxon:7955 denotes zebrafish
T15164 32392-32396 CHEBI_SO_EXT:mRNA denotes mRNA
T15165 32422-32429 CL:0000365 denotes zygotic
T15166 32430-32433 PR_EXT:Q6E2N3 denotes mon
T15167 32434-32441 SO_EXT:sequence_altered_entity denotes mutants
T15168 32501-32506 PR_EXT:000016655 denotes tif1γ
T15169 32524-32538 GO:0030218 denotes erythropoiesis
T15170 32588-32601 GO:0030097 denotes hematopoietic
T15171 32616-32629 GO:0030097 denotes hematopoietic
T15172 32643-32646 PR_EXT:Q6E2N3 denotes mon
T15173 32647-32654 SO_EXT:sequence_altered_entity denotes mutants
T15174 32677-32692 CL:0000764 denotes erythroid cells
T15175 32687-32692 CL_GO_EXT:cell denotes cells
T15176 32706-32711 NCBITaxon:10088 denotes mouse
T15177 32712-32717 PR_EXT:000007857 denotes Gata1
T15178 32727-32734 UBERON:0000922 denotes embryos
T15179 32739-32748 NCBITaxon:7955 denotes zebrafish
T15180 32761-32766 PR_EXT:000007857 denotes gata1
T15181 32768-32774 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T15182 32775-32782 UBERON:0000922 denotes embryos
T15183 32866-32873 SO_EXT:0000704 denotes genetic
T15184 32895-32898 PR_EXT:Q6E2N3 denotes mon
T15185 32903-32908 PR_EXT:000007857 denotes gata1
T15186 32937-32943 SO_EXT:sequence_rescue_process denotes rescue
T15187 32944-32958 GO:0030218 denotes erythropoiesis
T15188 32978-32983 PR_EXT:000007857 denotes gata1
T15189 32984-32988 CHEBI_SO_EXT:mRNA denotes mRNA
T15190 32994-32997 PR_EXT:Q6E2N3 denotes mon
T15191 33009-33015 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T15192 33016-33023 UBERON:0000922 denotes embryos
T15193 33041-33046 PR_EXT:000016655 denotes tif1γ
T15194 33047-33051 CHEBI_SO_EXT:mRNA denotes mRNA
T15195 33057-33062 PR_EXT:000007857 denotes gata1
T15196 33074-33081 UBERON:0000922 denotes embryos
T15197 33092-33098 SO_EXT:sequence_rescue_process denotes rescue
T15198 33099-33112 GO:0030097 denotes hematopoiesis
T15199 33181-33186 PR_EXT:000016655 denotes Tif1γ
T15200 33191-33196 PR_EXT:000007857 denotes Gata1
T15201 33197-33205 CHEBI_PR_EXT:protein denotes proteins
T15202 33235-33240 NCBITaxon_EXT:yeast denotes yeast
T15203 33317-33326 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T15204 33344-33349 SO_EXT:0000704 denotes genes
T15205 33357-33362 CL_GO_EXT:cell denotes cells
T15206 33423-33428 PR_EXT:000007857 denotes gata1
T15207 33433-33438 PR_EXT:000016655 denotes tif1γ
T15208 33511-33518 SO_EXT:0000704 denotes genetic
T15209 33538-33543 PR_EXT:000007857 denotes gata1
T15210 33548-33553 PR_EXT:000016655 denotes tif1γ
T15211 33575-33583 GO:0065007 denotes regulate
T15212 33584-33588 SO_EXT:0000704 denotes gene
T15213 33589-33602 GO_EXT:transcription denotes transcription
T15214 33610-33615 UBERON:0000178 denotes blood
T15215 33610-33621 CL:0000081 denotes blood cells
T15216 33616-33621 CL_GO_EXT:cell denotes cells
T16316 33645-33654 _FRAGMENT denotes Primitive
T16317 33670-33684 GO:0060319 denotes Erythropoiesis
T16318 33659-33684 GO:0060318 denotes Definitive Erythropoiesis
T16319 33723-33728 PR_EXT:000016655 denotes tif1γ
T16315 33636-33641 PR_EXT:000016655 denotes Tif1γ
T16320 33757-33765 CHEBI_EXT:23357 denotes cofactor
T16321 33774-33783 CL:0000764 denotes erythroid
T16322 33801-33811 _FRAGMENT denotes control of
T16323 33826-33841 GO:0010468 denotes gene expression
T16324 33812-33825 GO:0030097 denotes hematopoietic
T16325 33826-33830 SO_EXT:0000704 denotes gene
T16326 33870-33875 PR_EXT:000016655 denotes Tif1γ
T16327 33876-33883 CHEBI_PR_EXT:protein denotes protein
T16328 33899-33914 GO_EXT:transcription denotes transcriptional
T16329 33938-33951 GO:0030097 denotes hematopoietic
T16330 33938-33968 CL:0008001 denotes hematopoietic progenitor cells
T16331 33963-33968 CL_GO_EXT:cell denotes cells
T16332 33985-33990 PR_EXT:000016649 denotes TIF1α
T16333 34015-34020 PR_EXT:000016652 denotes TIF1β
T16334 34116-34126 _FRAGMENT denotes positively
T16335 34141-34149 GO_EXT:positive_regulation denotes regulate
T16336 34130-34149 GO_EXT:negative_regulation denotes negatively regulate
T16337 34150-34154 SO_EXT:0000704 denotes gene
T16338 34155-34168 GO_EXT:transcription denotes transcription
T16339 34198-34203 PR_EXT:000016649 denotes TIF1α
T16340 34208-34213 PR_EXT:000016652 denotes TIF1β
T16341 34221-34230 GO_EXT:0032947 denotes scaffolds
T16342 34262-34265 CHEBI_SO_EXT:DNA denotes DNA
T16343 34266-34273 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T16344 34274-34282 CHEBI_PR_EXT:protein denotes proteins
T16345 34287-34296 GO:0000785 denotes chromatin
T16346 34308-34316 CHEBI_PR_EXT:protein denotes proteins
T16347 34329-34339 GO:0065007 denotes regulatory
T16348 34340-34349 GO:0032991 denotes complexes
T16349 34351-34356 PR_EXT:000016655 denotes Tif1γ
T16350 34376-34383 GO:0005634 denotes nuclear
T16351 34504-34518 GO:0016604 denotes nuclear bodies
T16352 34530-34533 PR_EXT:000026474 denotes PML
T16353 34530-34540 GO:0016605 denotes PML bodies
T16354 34542-34554 GO_PATO_EXT:biological_localization_process_or_quality denotes Localization
T16355 34558-34563 PR_EXT:000016655 denotes Tif1γ
T16356 34573-34587 GO:0016604 denotes nuclear bodies
T16357 34595-34604 GO:0065007 denotes regulated
T16358 34608-34638 GO_SO_EXT:post_translational_modification_entity_or_process denotes posttranslational modification
T16359 34652-34664 SO_EXT:sequence_alteration_entity_or_process denotes modification
T16360 34686-34689 PR_EXT:000026474 denotes PML
T16361 34698-34701 PR_EXT:000026474 denotes PML
T16362 34698-34717 GO:0016605 denotes PML nuclear domains
T16363 34811-34816 PR_EXT:000016655 denotes Tif1γ
T16364 34836-34845 GO:0032991 denotes complexes
T16365 34851-34854 CHEBI_SO_EXT:DNA denotes DNA
T16366 34855-34862 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T16367 34863-34876 GO_EXT:transcription denotes transcription
T16368 34863-34884 GO_EXT:transcription_factor denotes transcription factors
T16369 34968-34977 CL:0000764 denotes erythroid
T16370 34978-34993 GO_RO_EXT:developmental_differentiation_process denotes differentiation
T16371 35045-35050 PR_EXT:000016655 denotes Tif1γ
T16372 35063-35071 CHEBI_PR_EXT:protein denotes proteins
T16373 35075-35082 GO:0005634 denotes nuclear
T16374 35125-35130 PR_EXT:000016655 denotes Tif1γ
T16375 35134-35142 GO:0065007 denotes regulate
T16376 35143-35148 UBERON:0000178 denotes blood
T16377 35143-35153 CL:0000081 denotes blood cell
T16378 35149-35153 CL_GO_EXT:cell denotes cell
T16379 35149-35165 GO:0048468 denotes cell development
T17018 35605-35616 SO_EXT:polymorphism denotes polymorphic
T17019 35679-35686 UBERON:0000922 denotes embryos
T17014 35391-35400 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T17015 35445-35448 PR_EXT:Q6E2N3 denotes mon
T17007 35191-35200 NCBITaxon:7955 denotes Zebrafish
T17020 35735-35748 GO:0097617 denotes hybridization
T17021 35766-35773 UBERON:0000922 denotes embryos
T17022 35847-35860 GO:0097617 denotes hybridization
T17023 35864-35869 NCBITaxon:10088 denotes mouse
T17024 35870-35877 UBERON:0000922 denotes embryos
T17025 35929-35940 SO_EXT:genomic_DNA denotes Genomic DNA
T17026 35937-35940 CHEBI_SO_EXT:DNA denotes DNA
T17027 35964-35968 SO_EXT:0001830 denotes AFLP
T17028 35983-35994 GO_SO_EXT:chromosome denotes chromosomal
T17029 36111-36118 SO_EXT:0000112 denotes primers
T17030 36123-36130 SO_EXT:0000704 denotes genetic
T17031 36140-36142 GO_EXT:reverse_transcription_or_reverse_transcriptase denotes RT
T17032 36166-36169 PR_EXT:Q6E2N3 denotes mon
T17326 36197-36201 CHEBI_SO_EXT:mRNA denotes mRNA
T17327 36202-36212 GO:0010467 denotes expression
T17328 36213-36223 SO_EXT:engineered_biological_sequence denotes constructs
T17329 36225-36236 SO:0001183 denotes morpholinos
T17330 36273-36276 PR_EXT:Q6E2N3 denotes mon
T17331 36277-36281 SO_EXT:cDNA denotes cDNA
T17332 36335-36341 SO_EXT:0000440 denotes vector
T17333 36353-36357 CHEBI_SO_EXT:mRNA denotes mRNA
T17334 36362-36373 GO_EXT:transcription denotes transcribed
T17619 36463-36467 CL_GO_EXT:cell denotes Cell
T17620 36490-36503 UBERON:0007132 denotes kidney marrow
T17621 36504-36509 CL_GO_EXT:cell denotes cells
T17622 36529-36534 UBERON:0007023 denotes adult
T17623 36535-36540 PR_EXT:000007857 denotes gata1
T17624 36546-36556 SO_EXT:transgenic_entity denotes transgenic
T17625 36585-36594 CHEBI_EXT:phosphate denotes phosphate
T17626 36595-36603 CHEBI_CHMO_EXT:buffer_process denotes buffered
T17627 36604-36610 CHEBI_EXT:saline_solution denotes saline
T17628 36622-36628 NCBITaxon:27592 denotes bovine
T17629 36629-36634 UBERON:0001977 denotes serum
T17630 36658-36671 UBERON:0002063 denotes sinus venosus
T17631 36683-36686 PR_EXT:Q6E2N3 denotes mon
T17632 36692-36693 SO_EXT:sequence_nullness_or_absence denotes −
T17633 36694-36695 SO_EXT:sequence_nullness_or_absence denotes −
T17634 36708-36715 UBERON:0000922 denotes embryos
T17635 36737-36750 UBERON:0007132 denotes kidney marrow
T17636 36751-36756 CL_GO_EXT:cell denotes cells
T17637 36775-36781 UBERON:0000922 denotes embryo
T17638 36807-36814 UBERON:0000922 denotes embryos
T17639 36820-36832 CHEBI_EXT:anaesthetic_process denotes anesthetized
T17640 36939-36944 CL_GO_EXT:cell denotes cells
T17641 37006-37012 UBERON:0002548 denotes larvae
T18155 37076-37086 GO:0042571 denotes Antibodies
T18156 37124-37128 UBERON:0001977 denotes sera
T18157 37141-37146 NCBITaxon:9606 denotes human
T18158 37147-37157 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T18159 37158-37163 PR_EXT:000016655 denotes TIF1γ
T18160 37164-37172 SO_EXT:biological_sequence denotes sequence
T18161 37206-37213 NCBITaxon:9986 denotes rabbits
T18162 37305-37310 NCBITaxon:10088 denotes Mouse
T18163 37311-37320 UBERON:0000922 denotes embryonic
T18164 37321-37332 CL:0000057 denotes fibroblasts
T18165 37333-37338 GO:0040007 denotes grown
T18166 37377-37381 PR_EXT:000005086 denotes HP1α
T18167 37434-37439 PR_EXT:000016655 denotes Tif1γ
T18168 37444-37448 UBERON:0001977 denotes sera
T18169 37475-37480 CL_GO_EXT:cell denotes cells
T18170 37498-37514 CHEBI_EXT:paraformaldehye denotes paraformaldehyde
T18171 37538-37547 CHEBI_EXT:phosphate denotes phosphate
T18172 37548-37556 CHEBI_CHMO_EXT:buffer_process denotes buffered
T18173 37557-37563 CHEBI_EXT:saline_solution denotes saline
T18174 37585-37590 UBERON:0001977 denotes serum
T18175 37644-37654 GO:0042571 denotes antibodies
T18176 37670-37675 CL_GO_EXT:cell denotes cells
T18177 37739-37749 GO:0042571 denotes antibodies
T18178 37839-37844 CL_GO_EXT:cell denotes Cells
T18179 37876-37880 CHEBI:51231 denotes DAPI
T18180 38071-38075 CL_GO_EXT:cell denotes cell
T18181 38071-38091 GO:0030154 denotes cell differentiation
T629 1056-1066 GO:0010467 denotes expression
T7810 16539-16547 UBERON:0011919 denotes yolk sac
T7811 16572-16581 GO:0010467 denotes expressed
T7812 16595-16600 UBERON:0002107 denotes liver
T7813 16631-16638 UBERON:0000479 denotes tissues
T7814 16654-16676 UBERON:0001017 denotes central nervous system
R248 T2618 T2617 _lexicallyChainedTo corepressors,transcriptional
R249 T2679 T2678 _lexicallyChainedTo hematopoiesis,embryonic
R1006 T4510 T4509 _lexicallyChainedTo Erythropoiesis,Primitive
R1007 T4562 T4561 _lexicallyChainedTo embryos,development of
R1008 T4592 T4591 _lexicallyChainedTo of,development
R1009 T4593 T4592 _lexicallyChainedTo cells,of
R1010 T4594 T4592 _lexicallyChainedTo mesenchymal cells,of
R1011 T4632 T4631 _lexicallyChainedTo cells,differentiation of
R1012 T4638 T4637 _lexicallyChainedTo erythropoiesis,primitive
R1488 T7633 T7632 _lexicallyChainedTo gene,predicted
R1489 T7660 T7659 _lexicallyChainedTo Chromosome,locus on
R1490 T7664 T7663 _lexicallyChainedTo Chromosome,region of
R1491 T7693 T7692 _lexicallyChainedTo cells,circulating
R1492 T7700 T7699 _lexicallyChainedTo cells,circulating
R1493 T7757 T7756 _lexicallyChainedTo mesoderm,ventral
R1494 T7780 T7779 _lexicallyChainedTo trunk,mesenchyme of
R1495 T7810 T7809 _lexicallyChainedTo yolk sac,blood islands of
R1496 T7853 T7852 _lexicallyChainedTo Chromosome,region of
R1497 T7872 T7871 _lexicallyChainedTo gene,predicted
R2627 T9582 T9581 _lexicallyChainedTo erythrocytes,formation of
R2628 T9589 T9588 _lexicallyChainedTo organogenesis,embryonic
R2846 T10617 T10616 _lexicallyChainedTo cells,embryonic
R3194 T11908 T11907 _lexicallyChainedTo nuclear bodies,promyelocytic leukemia gene product
R3195 T11908 T11911 _lexicallyChainedTo nuclear bodies,PML
R3196 T11923 T11922 _lexicallyChainedTo cells,differentiation of
R3197 T11946 T11945 _lexicallyChainedTo cells,maturation of
R3647 T13495 T13494 _lexicallyChainedTo genes,predicted
R3648 T13502 T13501 _lexicallyChainedTo Chromosome,region of
R3649 T13506 T13505 _lexicallyChainedTo Chromosome,region of
R3650 T13534 T13533 _lexicallyChainedTo embryos,hematopoiesis in
R4512 T16317 T16316 _lexicallyChainedTo Erythropoiesis,Primitive
R4513 T16323 T16322 _lexicallyChainedTo gene expression,control of
R4514 T16335 T16334 _lexicallyChainedTo regulate,positively
R4 T586 T585 _lexicallyChainedTo hematopoiesis,embryonic
R5 T635 T634 _lexicallyChainedTo formation,cell
R6 T655 T654 _lexicallyChainedTo cells,differentiation of
R242 T2514 T2513 _lexicallyChainedTo cells,differentiation of
R243 T2517 T2516 _lexicallyChainedTo cells,circulating
R244 T2519 T2518 _lexicallyChainedTo blood cells,red
R245 T2529 T2528 _lexicallyChainedTo cells,stem
R246 T2589 T2588 _lexicallyChainedTo hematopoiesis,primitive embryonic
R247 T2592 T2591 _lexicallyChainedTo hematopoiesis,definitive

2_test

Id Subject Object Predicate Lexical cue
15314655-11799232-84850863 2023-2027 11799232 denotes 2002
15314655-11495698-84850864 2297-2301 11495698 denotes 2001
15314655-11919568-84850865 2317-2321 11919568 denotes 2002
15314655-12510667-84850866 2340-2344 12510667 denotes 2003
15314655-11799232-84850867 3105-3109 11799232 denotes 2002
15314655-9007251-84850868 3340-3344 9007251 denotes 1996
15314655-9007250-84850869 3478-3482 9007250 denotes 1996
15314655-7744009-84850870 4058-4062 7744009 denotes 1995
15314655-8769649-84850871 4080-4084 8769649 denotes 1996
15314655-8986806-84850872 4097-4101 8986806 denotes 1996
15314655-10022127-84850873 4120-4124 10022127 denotes 1999
15314655-12096914-84850874 4138-4142 12096914 denotes 2002
15314655-11331580-84850875 4302-4306 11331580 denotes 2001
15314655-7744009-84850876 4628-4632 7744009 denotes 1995
15314655-10610177-84850877 4705-4709 10610177 denotes 1999
15314655-8769649-84850878 4869-4873 8769649 denotes 1996
15314655-11171966-84850879 4889-4893 11171966 denotes 2001
15314655-10022127-84850880 5050-5054 10022127 denotes 1999
15314655-11171966-84850881 5070-5074 11171966 denotes 2001
15314655-12096914-84850882 5215-5219 12096914 denotes 2002
15314655-10851139-84850883 5471-5475 10851139 denotes 2000
15314655-9007251-84850884 6675-6679 9007251 denotes 1996
15314655-9007250-84850885 6698-6702 9007250 denotes 1996
15314655-9007251-84850886 7662-7666 9007251 denotes 1996
15314655-9007251-84850887 9076-9080 9007251 denotes 1996
15314655-11468162-84850888 9540-9544 11468162 denotes 2001
15314655-9537415-84850889 10732-10736 9537415 denotes 1998
15314655-9891339-84850890 10886-10890 9891339 denotes 1999
15314655-7744009-84850891 13419-13423 7744009 denotes 1995
15314655-8769649-84850892 13441-13445 8769649 denotes 1996
15314655-10022127-84850893 13464-13468 10022127 denotes 1999
15314655-10984453-84850894 13868-13872 10984453 denotes 2000
15314655-9053314-84850895 15784-15788 9053314 denotes 1997
15314655-7479870-84850896 15918-15922 7479870 denotes 1995
15314655-12096914-84850897 18213-18217 12096914 denotes 2002
15314655-10984453-84850898 18877-18881 10984453 denotes 2000
15314655-7744009-84850899 19023-19027 7744009 denotes 1995
15314655-10525195-84850900 19050-19054 10525195 denotes 1999
15314655-9007251-84850901 20293-20297 9007251 denotes 1996
15314655-9374406-84850902 23018-23022 9374406 denotes 1997
15314655-14608381-84850903 24098-24102 14608381 denotes 2003
15314655-12384511-84850904 25503-25507 12384511 denotes 2002
15314655-12154074-84850905 25533-25537 12154074 denotes 2002
15314655-12154074-84850906 25693-25697 12154074 denotes 2002
15314655-9032291-84850907 26473-26477 9032291 denotes 1997
15314655-11799232-84850908 28040-28044 11799232 denotes 2002
15314655-12510667-84850909 28063-28067 12510667 denotes 2003
15314655-9007251-84850910 28259-28263 9007251 denotes 1996
15314655-7744009-84850911 29719-29723 7744009 denotes 1995
15314655-10525195-84850912 29746-29750 10525195 denotes 1999
15314655-14519192-84850913 30326-30330 14519192 denotes 2003
15314655-8901585-84850914 31751-31755 8901585 denotes 1996
15314655-11960002-84850915 31770-31774 11960002 denotes 2002
15314655-8901585-84850916 32800-32804 8901585 denotes 1996
15314655-11960002-84850917 32819-32823 11960002 denotes 2002
15314655-10610177-84850918 34005-34009 10610177 denotes 1999
15314655-8769649-84850919 34038-34042 8769649 denotes 1996
15314655-11171966-84850920 34058-34062 11171966 denotes 2001
15314655-12419228-84850922 34758-34762 12419228 denotes 2002
15314655-9007251-84850923 35416-35420 9007251 denotes 1996
15314655-9007250-84850924 35541-35545 9007250 denotes 1996
15314655-9630750-84850925 35814-35818 9630750 denotes 1998
15314655-11830566-84850926 35832-35836 11830566 denotes 2002
15314655-11284963-84850927 35922-35926 11284963 denotes 2001
15314655-9806542-84850928 36064-36068 9806542 denotes 1998
15314655-9891339-84850929 36085-36089 9891339 denotes 1999
15314655-11830566-84850930 36455-36459 11830566 denotes 2002
15314655-9032291-84850931 38158-38162 9032291 denotes 1997
T61321 2023-2027 11799232 denotes 2002
T66436 2297-2301 11495698 denotes 2001
T26376 2317-2321 11919568 denotes 2002
T28706 2340-2344 12510667 denotes 2003
T38698 3105-3109 11799232 denotes 2002
T79453 3340-3344 9007251 denotes 1996
T17364 3478-3482 9007250 denotes 1996
T38119 4058-4062 7744009 denotes 1995
T10831 4080-4084 8769649 denotes 1996
T58312 4097-4101 8986806 denotes 1996
T6696 4120-4124 10022127 denotes 1999
T3946 4138-4142 12096914 denotes 2002
T88168 4302-4306 11331580 denotes 2001
T7383 4628-4632 7744009 denotes 1995
T94976 4705-4709 10610177 denotes 1999
T71432 4869-4873 8769649 denotes 1996
T11609 4889-4893 11171966 denotes 2001
T20274 5050-5054 10022127 denotes 1999
T89459 5070-5074 11171966 denotes 2001
T65404 5215-5219 12096914 denotes 2002
T79898 5471-5475 10851139 denotes 2000
T83534 6675-6679 9007251 denotes 1996
T45649 6698-6702 9007250 denotes 1996
T71352 7662-7666 9007251 denotes 1996
T61688 9076-9080 9007251 denotes 1996
T39957 9540-9544 11468162 denotes 2001
T8360 10732-10736 9537415 denotes 1998
T42310 10886-10890 9891339 denotes 1999
T7158 13419-13423 7744009 denotes 1995
T2977 13441-13445 8769649 denotes 1996
T17868 13464-13468 10022127 denotes 1999
T71093 13868-13872 10984453 denotes 2000
T83510 15784-15788 9053314 denotes 1997
T89566 15918-15922 7479870 denotes 1995
T62215 18213-18217 12096914 denotes 2002
T45570 18877-18881 10984453 denotes 2000
T66506 19023-19027 7744009 denotes 1995
T25679 19050-19054 10525195 denotes 1999
T17518 20293-20297 9007251 denotes 1996
T65880 23018-23022 9374406 denotes 1997
T36261 24098-24102 14608381 denotes 2003
T8286 25503-25507 12384511 denotes 2002
T38592 25533-25537 12154074 denotes 2002
T35680 25693-25697 12154074 denotes 2002
T13610 26473-26477 9032291 denotes 1997
T34538 28040-28044 11799232 denotes 2002
T79917 28063-28067 12510667 denotes 2003
T87583 28259-28263 9007251 denotes 1996
T42963 29719-29723 7744009 denotes 1995
T71608 29746-29750 10525195 denotes 1999
T13741 30326-30330 14519192 denotes 2003
T26340 31751-31755 8901585 denotes 1996
T77771 31770-31774 11960002 denotes 2002
T19484 32800-32804 8901585 denotes 1996
T36846 32819-32823 11960002 denotes 2002
T90562 34005-34009 10610177 denotes 1999
T72754 34038-34042 8769649 denotes 1996
T58308 34058-34062 11171966 denotes 2001
T55913 34758-34762 12419228 denotes 2002
T53027 35416-35420 9007251 denotes 1996
T3580 35541-35545 9007250 denotes 1996
T38010 35814-35818 9630750 denotes 1998
T87520 35832-35836 11830566 denotes 2002
T90597 35922-35926 11284963 denotes 2001
T72839 36064-36068 9806542 denotes 1998
T16923 36085-36089 9891339 denotes 1999
T27309 36455-36459 11830566 denotes 2002
T22533 38158-38162 9032291 denotes 1997

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T502 4-13 NCBITaxon:7955 denotes Zebrafish
T503 14-23 PR:Q6E2N3 denotes moonshine
T504 24-28 SO:0000704 denotes Gene
T505 37-75 PR:000016655 denotes Transcriptional Intermediary Factor 1γ
T506 103-116 GO:0030097 denotes Hematopoiesis
T507 160-173 GO:0030097 denotes Hematopoiesis
T508 246-254 GO:0065007 denotes regulate
T509 333-342 NCBITaxon:7955 denotes zebrafish
T510 343-352 PR:Q6E2N3 denotes moonshine
T511 354-357 PR:Q6E2N3 denotes mon
T512 359-363 SO:0000704 denotes gene
T513 390-399 UBERON:0000922 denotes embryonic
T514 390-399 _FRAGMENT denotes embryonic
T515 410-423 GO:0035162 denotes hematopoiesis
T516 404-409 UBERON:0007023 denotes adult
T517 445-459 CL:0000232 denotes red blood cell
T518 449-454 UBERON:0000178 denotes blood
T519 484-487 PR:Q6E2N3 denotes mon
T520 500-509 NCBITaxon:7955 denotes zebrafish
T521 510-518 SO:0000855 denotes ortholog
T522 522-531 NCBITaxon:40674 denotes mammalian
T523 532-570 PR:000016655 denotes transcriptional intermediary factor 1γ
T524 572-577 PR:000016655 denotes TIF1γ
T525 583-589 PR:000016655 denotes TRIM33
T526 674-687 GO:0030097 denotes hematopoietic
T527 674-704 CL:0008001 denotes hematopoietic progenitor cells
T528 708-711 PR:Q6E2N3 denotes mon
T529 728-735 GO:0010467 denotes express
T530 753-766 GO:0030097 denotes hematopoietic
T531 800-805 PR:000007857 denotes gata1
T532 819-828 GO:0006915 denotes apoptosis
T533 846-849 PR:Q6E2N3 denotes mon
T534 857-864 SO:0001023 denotes alleles
T535 877-898 SO:0001587 denotes premature stop codons
T536 913-923 GO:0010467 denotes expression
T537 937-942 PR:000016655 denotes tif1γ
T538 956-965 UBERON:0000922 denotes embryonic
T539 956-979 GO:0035162 denotes embryonic hematopoiesis
T540 994-997 PR:Q6E2N3 denotes mon
T541 1037-1044 CL:0000365 denotes zygotic
T542 1045-1050 PR:000016655 denotes tif1γ
T543 1056-1066 GO:0010467 denotes expression
T544 1078-1094 UBERON:2000083 denotes ventral mesoderm
T545 1102-1115 GO:0030097 denotes hematopoietic
T546 1102-1125 CL:0000037 denotes hematopoietic stem cell
T547 1121-1125 _FRAGMENT denotes cell
T548 1141-1150 GO:0048468 denotes formation
T549 1160-1165 PR:000007857 denotes gata1
T551 1214-1219 PR:000016655 denotes tif1γ
T552 1288-1297 CL:0000764 denotes erythroid
T553 1321-1327 NCBITaxon:39107 denotes murine
T554 1328-1342 CL:0000764 denotes erythroid cell
T555 1366-1371 PR:000016655 denotes Tif1γ
T556 1406-1413 GO:0005634 denotes nuclear
T557 1424-1434 GO:0010467 denotes expression
T558 1452-1466 CL:0000764 denotes erythroid cell
T559 1462-1477 GO:0048469 denotes cell maturation
T560 1566-1584 _FRAGMENT denotes differentiation of
T561 1599-1604 GO:0030154 denotes cells
T562 1585-1598 GO:0030097 denotes hematopoietic
T563 1585-1604 CL:0000988 denotes hematopoietic cells
T564 1608-1619 NCBITaxon:7742 denotes vertebrates
T2448 3866-3869 PR:Q6E2N3 denotes mon
T2449 3870-3874 SO:0000704 denotes gene
T2450 3882-3891 NCBITaxon:7955 denotes zebrafish
T2451 3892-3900 SO:0000855 denotes ortholog
T2452 3904-3913 NCBITaxon:40674 denotes mammalian
T2375 1812-1825 GO:0030097 denotes Hematopoiesis
T2376 1864-1882 GO:0008283 denotes cell proliferation
T2377 1887-1905 _FRAGMENT denotes differentiation of
T2378 1946-1951 GO:0030154 denotes cells
T2379 1969-1980 _FRAGMENT denotes circulating
T2380 2001-2006 CL:0000080 denotes cells
T2381 1981-1984 _FRAGMENT denotes red
T2382 1995-2006 CL:0000232 denotes blood cells
T2383 1989-2006 CL:0000738 denotes white blood cells
T2384 1995-2000 UBERON:0000178 denotes blood
T2385 2030-2043 GO:0030097 denotes Hematopoiesis
T2386 2047-2058 NCBITaxon:7742 denotes vertebrates
T2387 2065-2074 NCBITaxon:7955 denotes zebrafish
T2388 2078-2084 NCBITaxon:9606 denotes humans
T2389 2152-2156 _FRAGMENT denotes stem
T2390 2171-2176 CL:0000034 denotes cells
T2391 2203-2212 UBERON:0000922 denotes embryonic
T2392 2259-2264 UBERON:0000178 denotes blood
T2393 2259-2270 CL:0000081 denotes blood cells
T2394 2380-2393 GO:0030097 denotes hematopoiesis
T2395 2410-2426 UBERON:2000083 denotes ventral mesoderm
T2396 2484-2493 UBERON:0000922 denotes embryonic
T2397 2494-2507 UBERON:0003061 denotes blood islands
T2398 2531-2544 GO:0030097 denotes hematopoiesis
T2399 2565-2574 UBERON:0000922 denotes embryonic
T2400 2575-2587 CL:0000232 denotes erythrocytes
T2401 2592-2603 CL:0000235 denotes macrophages
T2402 2640-2653 GO:0030097 denotes hematopoiesis
T2403 2680-2690 CL:0000034 denotes stem cells
T2404 2726-2735 UBERON:0000922 denotes embryonic
T2405 2736-2741 UBERON:0000947 denotes aorta
T2406 2742-2747 UBERON:0000991 denotes gonad
T2407 2748-2759 UBERON:0000080 denotes mesonephros
T2408 2774-2798 GO:0060216 denotes definitive hematopoietic
T2409 2785-2809 CL:0000037 denotes hematopoietic stem cells
T2410 2878-2883 UBERON:0007023 denotes adult
T2411 2884-2889 UBERON:0000178 denotes blood
T2412 2884-2895 CL:0000081 denotes blood cells
T2413 2918-2930 CL:0000232 denotes erythrocytes
T2414 2932-2945 CL:0000763 denotes myeloid cells
T2415 2951-2962 CL:0000542 denotes lymphocytes
T2416 2985-2992 SO:0000704 denotes genetic
T2417 3018-3023 SO:0000704 denotes genes
T2418 3029-3036 GO:0065007 denotes control
T2419 3037-3050 GO:0030097 denotes hematopoiesis
T2420 3061-3070 NCBITaxon:7955 denotes zebrafish
T2421 3145-3152 SO:0000704 denotes genetic
T2422 3186-3191 UBERON:0000178 denotes blood
T2423 3196-3205 NCBITaxon:7955 denotes zebrafish
T2424 3229-3236 GO:0010467 denotes express
T2425 3241-3250 CL:0000764 denotes erythroid
T2426 3272-3277 PR:000007857 denotes gata1
T2427 3290-3299 UBERON:0000922 denotes embryonic
T2428 3290-3313 GO:0035162 denotes embryonic hematopoietic
T2429 3369-3378 NCBITaxon:7955 denotes zebrafish
T2430 3379-3384 SO:0000704 denotes genes
T2431 3385-3394 PR:Q6E2N3 denotes moonshine
T2432 3396-3399 PR:Q6E2N3 denotes mon
T2433 3445-3451 SO:0001023 denotes allele
T2434 3532-3535 PR:Q6E2N3 denotes mon
T2435 3536-3540 SO:0000704 denotes gene
T2436 3568-3587 _FRAGMENT denotes primitive embryonic
T2437 3609-3622 GO:0060215 denotes hematopoiesis
T2438 3578-3587 UBERON:0000922 denotes embryonic
T2439 3592-3602 _FRAGMENT denotes definitive
T2440 3609-3622 GO:0060216 denotes hematopoiesis
T2441 3603-3608 UBERON:0007023 denotes adult
T2442 3654-3669 CL:0000764 denotes erythroid cells
T2443 3671-3697 CL:0000038 denotes Erythroid progenitor cells
T2444 3701-3704 PR:Q6E2N3 denotes mon
T2445 3748-3755 GO:0010467 denotes express
T2446 3773-3786 GO:0030097 denotes hematopoietic
T2447 3821-3830 GO:0006915 denotes apoptosis
T2453 3914-3952 PR:000016655 denotes transcriptional intermediary factor 1γ
T2454 3954-3959 PR:000016655 denotes TIF1γ
T2455 4170-4180 NCBITaxon:7742 denotes vertebrate
T2456 4194-4195 PR:000016649 denotes α
T2457 4197-4198 PR:000016652 denotes β
T2458 4204-4205 PR:000016655 denotes γ
T2459 4217-4224 GO:0005634 denotes nuclear
T2460 4279-4285 SO:0000417 denotes domain
T2461 4354-4365 SO:0001080 denotes coiled-coil
T2462 4366-4372 SO:0000417 denotes domain
T2463 4420-4425 NCBITaxon:3193 denotes plant
T2464 4488-4497 GO:0000785 denotes chromatin
T2465 4488-4508 GO:0006338 denotes chromatin remodeling
T2466 4518-4523 PR:000016649 denotes TIF1α
T2467 4583-4590 GO:0005634 denotes nuclear
T2468 4668-4681 CHEBI:26536 denotes retinoic acid
T2469 4712-4717 PR:000016652 denotes TIF1β
T2470 4811-4817 SO:0000417 denotes domain
T2471 4909-4914 PR:000016655 denotes TIF1γ
T2472 4955-4962 GO:0005634 denotes nuclear
T2473 4981-4988 SO:0000417 denotes domains
T2474 5119-5124 PR:000016655 denotes TIF1γ
T2475 5177-5182 PR:000016649 denotes TIF1α
T2476 5196-5201 PR:000016652 denotes TIF1β
T2477 5226-5232 NCBITaxon:39107 denotes murine
T2478 5233-5238 PR:000016649 denotes Tif1α
T2479 5243-5248 PR:000016655 denotes Tif1γ
T2480 5249-5254 SO:0000704 denotes genes
T2481 5286-5290 SO:0000704 denotes gene
T2482 5334-5339 NCBITaxon:10088 denotes mouse
T2483 5366-5371 PR:000016652 denotes Tif1β
T2484 5392-5404 GO:0007566 denotes implantation
T2485 5405-5418 GO:0009790 denotes embryogenesis
T2486 5423-5431 UBERON:0000926 denotes mesoderm
T2487 5680-5685 PR:000016655 denotes tif1γ
T2488 5725-5734 UBERON:0000922 denotes embryonic
T2489 5725-5734 _FRAGMENT denotes embryonic
T2490 5745-5758 GO:0035162 denotes hematopoiesis
T2491 5739-5744 UBERON:0007023 denotes adult
T2492 5762-5773 NCBITaxon:7742 denotes vertebrates
T2493 5821-5826 PR:000016655 denotes tif1γ
T2494 5867-5876 UBERON:0000922 denotes embryonic
T2495 5867-5890 GO:0035162 denotes embryonic hematopoiesis
T2496 5896-5901 PR:000016655 denotes tif1γ
T2497 5902-5906 SO:0000704 denotes gene
T2498 5910-5919 GO:0010467 denotes expressed
T2499 5936-5952 UBERON:2000083 denotes ventral mesoderm
T2500 5957-5970 GO:0030097 denotes hematopoietic
T2501 6006-6015 CL:0000764 denotes erythroid
T2502 6035-6040 PR:000016655 denotes Tif1γ
T2503 6079-6093 GO:0016604 denotes nuclear bodies
T2504 6110-6115 NCBITaxon:10088 denotes mouse
T2505 6116-6122 UBERON:0000922 denotes embryo
T2506 6123-6134 CL:0000057 denotes fibroblasts
T2507 6212-6217 PR:000016655 denotes Tif1γ
T2508 6241-6255 CL:0000764 denotes erythroid cell
T2509 6251-6267 GO:0048468 denotes cell development
T4430 6824-6831 UBERON:0000922 denotes embryos
T4431 6904-6919 CL:0000764 denotes erythroid cells
T4432 6928-6949 GO:0012501 denotes programmed cell death
T4433 6962-6968 UBERON:0002329 denotes somite
T4434 6987-7000 GO:0009566 denotes fertilization
T4435 7041-7048 UBERON:0002329 denotes somites
T4403 4-13 NCBITaxon:7955 denotes Zebrafish
T4404 14-6309 PR:Q6E2N3 denotes moonshine Gene Encodes Transcriptional Intermediary Factor 1γ, an Essential Regulator of Hematopoiesis moonshine/tif1γ in Hematopoiesis Abstract Hematopoiesis is precisely orchestrated by lineage-specific DNA-binding proteins that regulate transcription in concert with coactivators and corepressors. Mutations in the zebrafish moonshine (mon) gene specifically disrupt both embryonic and adult hematopoiesis, resulting in severe red blood cell aplasia. We report that mon encodes the zebrafish ortholog of mammalian transcriptional intermediary factor 1γ (TIF1γ) (or TRIM33), a member of the TIF1 family of coactivators and corepressors. During development, hematopoietic progenitor cells in mon mutants fail to express normal levels of hematopoietic transcription factors, including gata1, and undergo apoptosis. Three different mon mutant alleles each encode premature stop codons, and enforced expression of wild-type tif1γ mRNA rescues embryonic hematopoiesis in homozygous mon mutants. Surprisingly, a high level of zygotic tif1γ mRNA expression delineates ventral mesoderm during hematopoietic stem cell and progenitor formation prior to gata1 expression. Transplantation studies reveal that tif1γ functions in a cell-autonomous manner during the differentiation of erythroid precursors. Studies in murine erythroid cell lines demonstrate that Tif1γ protein is localized within novel nuclear foci, and expression decreases during erythroid cell maturation. Our results establish a major role for this transcriptional intermediary factor in the differentiation of hematopoietic cells in vertebrates. A new gene acting early in red blood cell development is discovered by genetic analysis in zebrafish. The gene encodes a member of a well-known family of transcription factors Introduction Hematopoiesis involves the coordinated processes of cell proliferation and differentiation of a relatively small number of progenitor cells into billions of circulating red and white blood cells (Thisse and Zon 2002). Hematopoiesis in vertebrates, from zebrafish to humans, is an evolutionarily conserved program that produces two waves of stem or progenitor cells that differ both in their embryonic origins and in the lineages of differentiated blood cells produced (Palis and Yoder 2001; Orkin and Zon 2002; Galloway and Zon 2003). The first, or primitive, wave of hematopoiesis originates from ventral mesoderm and gives rise to progenitor cells that differentiate in embryonic blood islands. The primitive wave of hematopoiesis produces a burst of embryonic erythrocytes and macrophages. The second, or definitive, wave of hematopoiesis arises from self-renewing stem cells that develop primarily in the intraembryonic aorta–gonad–mesonephros region. These definitive hematopoietic stem cells seed the later developing marrow spaces, to produce all lineages of adult blood cells, including definitive erythrocytes, myeloid cells, and lymphocytes. We have undertaken a genetic approach to characterize genes that control hematopoiesis using the zebrafish as a model system (Thisse and Zon 2002). As part of a large-scale forward genetic screen, we previously identified bloodless zebrafish mutants that failed to express the erythroid transcription factor gata1 normally in embryonic hematopoietic precursors (Ransom et al. 1996). We named one of these zebrafish genes moonshine (mon), and another group named a noncomplementing allele vampire (Weinstein et al. 1996). Here, we have determined that mutations in the mon gene cause a disruption in both primitive embryonic and definitive adult hematopoiesis, resulting in a severe loss of erythroid cells. Erythroid progenitor cells in mon mutants are initially present, but fail to express normal levels of hematopoietic transcription factors and undergo apoptosis. Positional cloning identifies the mon gene as the zebrafish ortholog of mammalian transcriptional intermediary factor 1γ (TIF1γ), a member of the TIF1 family of transcriptional coactivators and corepressors (Le Douarin et al. 1995; Friedman et al. 1996; Kim et al. 1996; Venturini et al. 1999; Peng et al. 2002). The three members of the vertebrate TIF1 family (α, β, and γ) are large nuclear proteins that each contain an N-terminal RBCC or TRIM domain (Reymond et al. 2001) composed of a RING finger, two B-boxes, and a coiled-coil domain. TIF1 family members also contain a C-terminal plant homeodomain finger and bromodomain that are characteristic of chromatin remodeling factors. TIF1α has been shown to associate with a variety of ligand-bound nuclear hormone receptors (Le Douarin et al. 1995) and function as a coactivator for retinoic acid receptors (Zhong et al.1999). TIF1β has been shown to act as a corepressor for the large family of Krüppel-associated box (KRAB) domain zinc-finger transcription factors (Friedman et al. 1996; Abrink et al. 2001). In contrast, TIF1γ does not associate directly with either nuclear receptors or KRAB domains that bind to the other TIF1 family members (Venturini et al. 1999; Abrink et al. 2001). Biochemical studies also demonstrate that TIF1γ forms both homo-oligomers and hetero-oligomers with TIF1α but not with TIF1β (Peng et al. 2002). The murine Tif1α and Tif1γ genes have not yet been subjected to gene targeting experiments, whereas analysis of mouse mutants demonstrates that Tif1β is required for postimplantation embryogenesis and mesoderm induction in particular (Cammas et al. 2000). Taken together, these studies suggest that a major function of TIF1 family members is to link DNA-binding proteins with other coactivators or corepressors during development. Our studies establish that tif1γ functions as an essential regulator of embryonic and adult hematopoiesis in vertebrates. Cell transplantation studies demonstrate that tif1γ acts in a cell-autonomous manner during embryonic hematopoiesis. The tif1γ gene is expressed specifically in ventral mesoderm and hematopoietic progenitors, then downregulated as erythroid maturation occurs. Tif1γ protein localizes to a novel class of nuclear bodies in both primary mouse embryo fibroblasts and erythroleukemia cell lines. Taken together, our studies demonstrate that Tif1γ is required for normal erythroid cell development and survival. Results The Zebrafish mon
T4405 6310-6314 SO:0000704 denotes Gene
T4406 6337-6346 _FRAGMENT denotes Primitive
T4407 6362-6376 GO:0060319 denotes Erythropoiesis
T4408 6351-6376 GO:0060318 denotes Definitive Erythropoiesis
T4409 6408-6411 PR:Q6E2N3 denotes mon
T4410 6412-6416 SO:0000704 denotes gene
T4411 6463-6476 GO:0030097 denotes hematopoietic
T4412 6477-6481 SO:0000704 denotes gene
T4413 6477-6492 GO:0010467 denotes gene expression
T4414 6497-6506 GO:0006915 denotes apoptosis
T4415 6510-6519 NCBITaxon:7955 denotes zebrafish
T4416 6531-6534 PR:Q6E2N3 denotes mon
T4417 6542-6549 UBERON:0000922 denotes embryos
T4418 6558-6571 GO:0009790 denotes embryogenesis
T4419 6584-6593 NCBITaxon:7955 denotes zebrafish
T4420 6594-6597 PR:Q6E2N3 denotes mon
T4421 6614-6629 CL:0000232 denotes red blood cells
T4422 6618-6623 UBERON:0000178 denotes blood
T4423 6631-6635 CL:0000232 denotes RBCs
T4424 6709-6712 PR:Q6E2N3 denotes mon
T4425 6730-6740 GO:0010467 denotes expression
T4426 6744-6749 PR:000007857 denotes gata1
T4427 6753-6766 GO:0030097 denotes hematopoietic
T4428 6753-6772 CL:0000988 denotes hematopoietic cells
T4429 6789-6795 UBERON:0002329 denotes somite
T4436 7050-7055 PR:000007857 denotes gata1
T4437 7056-7066 GO:0010467 denotes expression
T4438 7107-7120 GO:0030097 denotes hematopoietic
T4439 7146-7151 PR:000007857 denotes gata1
T4440 7153-7156 PR:000016043 denotes scl
T4441 7158-7163 PR:000007858 denotes gata2
T4442 7169-7175 PR:000001811 denotes ikaros
T4443 7200-7209 UBERON:0000922 denotes embryonic
T4444 7210-7222 UBERON:0003061 denotes blood island
T4445 7265-7278 GO:0030097 denotes hematopoietic
T4446 7265-7284 CL:0000988 denotes hematopoietic cells
T4447 7331-7345 _FRAGMENT denotes development of
T4448 7357-7364 GO:0009790 denotes embryos
T4449 7346-7349 PR:Q6E2N3 denotes mon
T4450 7357-7364 UBERON:0000922 denotes embryos
T4451 7395-7405 GO:0008219 denotes cell death
T4452 7416-7426 GO:0010467 denotes expression
T4453 7430-7435 PR:000010799 denotes c-myb
T4454 7440-7444 PR:000003457 denotes rag1
T4455 7466-7469 PR:Q6E2N3 denotes mon
T4456 7490-7497 CL:0000763 denotes myeloid
T4457 7568-7572 CL:0000232 denotes RBCs
T4458 7576-7579 PR:Q6E2N3 denotes mon
T4459 7618-7621 UBERON:0008897 denotes fin
T4460 7631-7635 UBERON:0002415 denotes tail
T4461 7636-7646 UBERON:0003104 denotes mesenchyme
T4462 7687-7690 PR:Q6E2N3 denotes mon
T4463 7722-7752 GO:0097152 denotes apoptosis of mesenchymal cells
T4464 7735-7746 UBERON:0003104 denotes mesenchymal
T4465 7735-7752 CL:0000134 denotes mesenchymal cells
T4466 7760-7765 UBERON:0002100 denotes trunk
T4467 7770-7778 UBERON:0002533 denotes tail bud
T4468 7819-7822 PR:Q6E2N3 denotes mon
T4469 7823-7827 SO:0000704 denotes gene
T4470 7856-7867 _FRAGMENT denotes development
T4471 7881-7883 _FRAGMENT denotes of
T4472 7920-7925 GO:0048468 denotes cells
T4473 7940-7957 GO:0014031 denotes mesenchymal cells
T4474 7899-7925 CL:0000038 denotes erythroid progenitor cells
T4475 7940-7951 UBERON:0003104 denotes mesenchymal
T4476 7940-7957 CL:0000134 denotes mesenchymal cells
T4477 8911-8935 GO:0060216 denotes definitive hematopoiesis
T4478 8965-8970 UBERON:0007023 denotes adult
T4479 8971-8980 NCBITaxon:7955 denotes zebrafish
T4480 8981-8984 PR:Q6E2N3 denotes mon
T4481 9007-9010 PR:Q6E2N3 denotes mon
T4482 9097-9100 PR:Q6E2N3 denotes mon
T4483 9144-9149 UBERON:0000178 denotes blood
T4484 9154-9161 UBERON:0000922 denotes embryos
T4485 9177-9184 SO:0001023 denotes alleles
T4486 9196-9205 UBERON:0000113 denotes adulthood
T4487 9207-9212 UBERON:0007023 denotes Adult
T4488 9213-9216 PR:Q6E2N3 denotes mon
T4489 9230-9237 UBERON:0000948 denotes cardiac
T4490 9343-9352 NCBITaxon:7955 denotes zebrafish
T4491 9358-9363 UBERON:0007023 denotes adult
T4492 9372-9385 GO:0030097 denotes hematopoiesis
T4493 9393-9399 UBERON:0002113 denotes kidney
T4494 9442-9451 CL:0000764 denotes erythroid
T4495 9467-9474 CL:0000763 denotes myeloid
T4496 9550-9553 PR:Q6E2N3 denotes mon
T4497 9619-9634 CL:0000547 denotes proerythroblast
T4498 9665-9683 _FRAGMENT denotes differentiation of
T4499 9692-9697 GO:0030154 denotes cells
T4500 9684-9697 CL:0000763 denotes myeloid cells
T4501 9755-9758 PR:Q6E2N3 denotes mon
T4502 9759-9763 SO:0000704 denotes gene
T4503 9789-9798 _FRAGMENT denotes primitive
T4504 9814-9828 GO:0060319 denotes erythropoiesis
T4505 9803-9828 GO:0060318 denotes definitive erythropoiesis
T7427 14318-14329 CL:0000081 denotes blood cells
T7428 14382-14386 CL:0000232 denotes RBCs
T7429 14435-14442 UBERON:0000922 denotes embryos
T7430 14471-14474 PR:Q6E2N3 denotes mon
T7431 14479-14485 SO:0001023 denotes allele
T7432 14508-14528 SO:0001587 denotes premature stop codon
T7433 14679-14682 PR:Q6E2N3 denotes mon
T7434 14683-14687 SO:0000704 denotes gene
T7435 14773-14784 GO:0006412 denotes translation
T7436 14800-14812 CL:0000558 denotes reticulocyte
T7437 14849-14860 GO:0006412 denotes translation
T7438 14929-14935 UBERON:0002548 denotes larval
T7439 14953-14956 PR:Q6E2N3 denotes mon
T7440 14961-14967 SO:0001023 denotes allele
T7441 15001-15004 PR:Q6E2N3 denotes mon
T7442 15017-15027 GO:0010467 denotes expression
T7443 15070-15073 PR:Q6E2N3 denotes mon
T7349 10374-10377 PR:Q6E2N3 denotes mon
T7350 10385-10394 NCBITaxon:7955 denotes Zebrafish
T7351 10395-10403 SO:0000855 denotes Ortholog
T7352 10407-10416 NCBITaxon:40674 denotes Mammalian
T7353 10417-10422 PR:000016655 denotes TIF1γ
T7354 10441-10444 PR:Q6E2N3 denotes mon
T7355 10445-10449 SO:0000704 denotes gene
T7356 10590-10593 PR:Q6E2N3 denotes mon
T7357 10599-10605 SO:0001023 denotes allele
T7358 10611-10614 PR:Q6E2N3 denotes mon
T7359 10622-10626 SO:0000704 denotes gene
T7360 10824-10862 SO:0001830 denotes amplified fragment length polymorphism
T7361 10864-10868 SO:0001830 denotes AFLP
T7362 10994-10998 SO:0000704 denotes gene
T7363 11084-11118 SO:0000154 denotes P1 bacterial artificial chromosome
T7364 11087-11096 NCBITaxon:2 denotes bacterial
T7365 11126-11129 SO:0000154 denotes PAC
T7366 11170-11177 SO:0000704 denotes genetic
T7367 11263-11266 SO:0000154 denotes PAC
T7368 11273-11278 SO:0000357 denotes flank
T7369 11292-11299 SO:0000704 denotes genetic
T7370 11365-11372 GO:0007126 denotes meioses
T7371 11523-11526 SO:0000154 denotes PAC
T7372 11551-11560 NCBITaxon:7955 denotes zebrafish
T7373 11561-11568 SO:0001026 denotes genomic
T7374 11578-11584 SO:0000149 denotes contig
T7375 11645-11654 _FRAGMENT denotes predicted
T7376 11677-11681 SO:0000996 denotes gene
T7377 11655-11664 NCBITaxon:7955 denotes zebrafish
T7378 11688-11691 SO:0000154 denotes PAC
T7379 11696-11706 GO:0097617 denotes hybridized
T7380 11712-11718 UBERON:0002113 denotes kidney
T7381 11801-11805 SO:0000704 denotes gene
T7382 13249-13252 PR:Q6E2N3 denotes mon
T7383 13253-13257 SO:0000704 denotes gene
T7384 13313-13322 CHEBI:23357 denotes cofactors
T7385 13366-13369 PR:Q6E2N3 denotes mon
T7386 13389-13394 NCBITaxon:9606 denotes human
T7387 13395-13399 PR:000016655 denotes TIFγ
T7388 13492-13507 SO:0000333 denotes exon boundaries
T7389 13534-13543 NCBITaxon:7955 denotes zebrafish
T7390 13548-13553 NCBITaxon:9606 denotes human
T7391 13554-13559 SO:0000704 denotes genes
T7392 13584-13587 PR:Q6E2N3 denotes mon
T7393 13597-13606 NCBITaxon:7955 denotes zebrafish
T7394 13644-13652 SO:0000860 denotes syntenic
T7395 13670-13675 NCBITaxon:9606 denotes human
T7396 13708-13713 PR:000016655 denotes TIF1γ
T7397 13714-13718 SO:0000704 denotes gene
T7398 13764-13775 SO:0000858 denotes orthologous
T7399 13776-13780 SO:0000704 denotes gene
T7400 13798-13802 PR:000011416 denotes NRAS
T7401 13831-13836 NCBITaxon:9606 denotes human
T7402 13841-13850 NCBITaxon:7955 denotes zebrafish
T7403 13945-13954 NCBITaxon:7955 denotes zebrafish
T7404 13955-13958 PR:Q6E2N3 denotes mon
T7405 13959-13963 SO:0000704 denotes gene
T7406 13978-13986 SO:0000855 denotes ortholog
T7407 13994-13999 NCBITaxon:9606 denotes human
T7408 14000-14005 PR:000016655 denotes TIF1γ
T7409 14006-14010 SO:0000704 denotes gene
T7410 14031-14048 CHEBI:23995 denotes ethyl-nitrosourea
T7411 14050-14053 CHEBI:23995 denotes ENU
T7412 14088-14095 SO:0001023 denotes alleles
T7413 14099-14102 PR:Q6E2N3 denotes mon
T7414 14149-14169 SO:0001587 denotes premature stop codon
T7415 14175-14178 PR:Q6E2N3 denotes mon
T7416 14189-14192 PR:Q6E2N3 denotes mon
T7417 14198-14205 SO:0001023 denotes alleles
T7418 14238-14249 _FRAGMENT denotes circulating
T7419 14256-14261 CL:0000080 denotes cells
T7420 14250-14255 UBERON:0000178 denotes blood
T7421 14250-14261 CL:0000081 denotes blood cells
T7422 14280-14283 PR:Q6E2N3 denotes mon
T7423 14288-14294 SO:0001023 denotes allele
T7424 14306-14317 _FRAGMENT denotes circulating
T7425 14324-14329 CL:0000080 denotes cells
T7426 14318-14323 UBERON:0000178 denotes blood
T7444 15074-15081 SO:0001023 denotes alleles
T7445 15107-15112 PR:000016655 denotes Tif1γ
T7446 15142-15145 PR:Q6E2N3 denotes mon
T7447 15187-15192 PR:000016655 denotes tif1γ
T7448 15196-15205 GO:0010467 denotes expressed
T7449 15209-15222 GO:0030097 denotes hematopoietic
T7450 15223-15231 UBERON:0000926 denotes mesoderm
T7451 15250-15259 NCBITaxon:7955 denotes zebrafish
T7452 15260-15267 UBERON:0000922 denotes embryos
T7453 15291-15304 GO:0097617 denotes hybridization
T7454 15318-15323 PR:000016655 denotes tif1γ
T7455 15332-15341 GO:0010467 denotes expressed
T7456 15381-15387 UBERON:0000922 denotes embryo
T7457 15395-15410 UBERON:0000108 denotes blastula stages
T7458 15419-15431 GO:0007369 denotes gastrulation
T7459 15436-15443 GO:0090504 denotes epiboly
T7460 15452-15459 CL:0000365 denotes zygotic
T7461 15460-15470 GO:0010467 denotes expression
T7462 15474-15477 PR:Q6E2N3 denotes mon
T7463 15515-15524 UBERON:0002541 denotes germ ring
T7464 15529-15537 UBERON:0002533 denotes tail bud
T7465 15548-15554 UBERON:0002329 denotes somite
T7466 15578-15583 PR:000016655 denotes tif1γ
T7467 15584-15594 GO:0010467 denotes expression
T7468 15639-15646 _FRAGMENT denotes ventral
T7469 15655-15663 UBERON:2000083 denotes mesoderm
T7470 15647-15663 UBERON:0003081 denotes lateral mesoderm
T7471 15687-15692 UBERON:0000178 denotes blood
T7472 15702-15711 GO:0010467 denotes expresses
T7473 15712-15721 CL:0000034 denotes stem cell
T7474 15712-15764 PR:000016043 denotes stem cell leukemiahematopoietic transcription factor
T7475 15730-15743 GO:0030097 denotes hematopoietic
T7476 15766-15769 PR:000016043 denotes scl
T7477 15824-15831 GO:0010467 denotes express
T7478 15832-15837 PR:000016655 denotes tif1γ
T7479 15842-15845 PR:000016043 denotes scl
T7480 15879-15888 UBERON:0000922 denotes embryonic
T7481 15889-15901 UBERON:0003061 denotes blood island
T7482 15929-15934 PR:000016655 denotes tif1γ
T7483 15935-15939 SO:0000704 denotes gene
T7484 15955-15964 GO:0010467 denotes expressed
T7485 15972-15994 UBERON:0001017 denotes central nervous system
T7486 16010-16023 _FRAGMENT denotes mesenchyme of
T7487 16028-16033 UBERON:0005256 denotes trunk
T7488 16038-16042 UBERON:0002415 denotes tail
T7489 16055-16058 PR:Q6E2N3 denotes mon
T7490 16105-16110 PR:000016655 denotes tif1γ
T7491 16123-16130 UBERON:0000479 denotes tissues
T7492 16147-16178 GO:0000184 denotes nonsense-mediated message decay
T7493 16165-16172 SO:0000673 denotes message
T7494 16165-16172 CHEBI:33695 denotes message
T7495 16186-16195 NCBITaxon:7955 denotes zebrafish
T7496 16196-16201 PR:000016655 denotes tif1γ
T7497 16218-16227 GO:0010467 denotes expressed
T7498 16231-16247 UBERON:2000083 denotes ventral mesoderm
T7499 16300-16309 UBERON:0000922 denotes embryonic
T7500 16322-16335 GO:0030097 denotes hematopoietic
T7501 16363-16372 GO:0006915 denotes apoptosis
T7502 16376-16379 PR:Q6E2N3 denotes mon
T7503 16410-16420 GO:0010467 denotes expression
T7504 16424-16433 NCBITaxon:7955 denotes zebrafish
T7505 16434-16437 PR:Q6E2N3 denotes mon
T7506 16441-16446 NCBITaxon:10088 denotes mouse
T7507 16447-16452 PR:000016655 denotes Tif1γ
T7508 16473-16478 NCBITaxon:10088 denotes Mouse
T7509 16479-16484 PR:000016655 denotes Tif1γ
T7510 16495-16504 GO:0010467 denotes expressed
T7511 16508-16517 CL:0000764 denotes erythroid
T7512 16518-16534 _FRAGMENT denotes blood islands of
T7513 16539-16547 UBERON:0011919 denotes yolk sac
T7514 16572-16581 GO:0010467 denotes expressed
T7515 16595-16600 UBERON:0002107 denotes liver
T7516 16631-16638 UBERON:0000479 denotes tissues
T7517 16654-16676 UBERON:0001017 denotes central nervous system
T7518 16729-16738 NCBITaxon:7955 denotes zebrafish
T7519 16739-16742 PR:Q6E2N3 denotes mon
T7520 16747-16752 NCBITaxon:10088 denotes mouse
T7521 16753-16758 PR:000016655 denotes Tif1γ
T7522 16763-16772 SO:0000855 denotes orthologs
T7523 16794-16807 GO:0030097 denotes hematopoiesis
T7524 18133-18142 NCBITaxon:40674 denotes mammalian
T7525 18143-18148 PR:000016655 denotes TIF1γ
T7526 18194-18199 PR:000016649 denotes Tif1α
T7527 18270-18279 NCBITaxon:7955 denotes zebrafish
T7528 18296-18301 PR:000016655 denotes tif1γ
T7529 18309-18318 NCBITaxon:7955 denotes zebrafish
T7530 18319-18328 GO:0010467 denotes expressed
T7531 18319-18341 SO:0000345 denotes expressed sequence tag
T7532 18343-18346 SO:0000345 denotes EST
T7533 18371-18378 SO:0000112 denotes primers
T7534 18422-18431 UBERON:0000922 denotes embryonic
T7535 18485-18494 NCBITaxon:7955 denotes zebrafish
T7536 18495-18503 SO:0000855 denotes ortholog
T7537 18507-18512 NCBITaxon:9606 denotes human
T7538 18513-18518 PR:000016649 denotes TIF1α
T7539 18589-18598 NCBITaxon:7955 denotes zebrafish
T7540 18599-18604 PR:000016649 denotes tif1α
T7541 18605-18609 SO:0000345 denotes ESTs
T9529 19533-19543 GO:0010467 denotes Expression
T9530 19547-19552 PR:000016655 denotes tif1γ
T9531 19561-19574 GO:0030097 denotes Hematopoiesis
T9532 19578-19581 PR:Q6E2N3 denotes mon
T9533 19632-19641 NCBITaxon:7955 denotes zebrafish
T9534 19642-19647 PR:000016655 denotes tif1γ
T9535 19648-19652 SO:0000704 denotes gene
T9536 19676-19679 PR:Q6E2N3 denotes mon
T9537 19710-19716 UBERON:0000922 denotes embryo
T9538 19796-19799 PR:Q6E2N3 denotes mon
T9539 19839-19851 _FRAGMENT denotes formation of
T9540 19862-19874 GO:0048821 denotes erythrocytes
T9541 19852-19861 UBERON:0000922 denotes embryonic
T9542 19862-19874 CL:0000232 denotes erythrocytes
T9543 19895-19902 UBERON:0000922 denotes embryos
T9544 19938-19947 UBERON:0000922 denotes embryonic
T9545 19938-19947 _FRAGMENT denotes embryonic
T9546 19962-19975 GO:0048568 denotes organogenesis
T9547 20016-20019 PR:Q6E2N3 denotes mon
T9548 20165-20169 CL:0000232 denotes RBCs
T9549 20259-20262 UBERON:0008897 denotes fin
T9550 20304-20315 UBERON:0003104 denotes mesenchymal
T9551 20304-20321 CL:0000134 denotes mesenchymal cells
T9552 20392-20402 GO:0010467 denotes expression
T9553 20406-20409 PR:Q6E2N3 denotes mon
T9554 20437-20442 UBERON:0000178 denotes blood
T9555 20437-20447 CL:0000081 denotes blood cell
T9556 20469-20476 UBERON:0000922 denotes embryos
T9557 20533-20536 PR:Q6E2N3 denotes mon
T9558 20605-20610 UBERON:0000178 denotes blood
T9559 20630-20637 UBERON:0000922 denotes embryos
T9560 20677-20680 PR:Q6E2N3 denotes mon
T9561 20727-20740 GO:0030097 denotes hematopoiesis
T10565 23746-23757 CL:0000081 denotes blood cells
T10566 23799-23803 GO:0036268 denotes swim
T10567 23799-23812 UBERON:0006860 denotes swim bladders
T10568 23890-23895 GO:0016265 denotes dying
T10524 22473-22487 GO:0030218 denotes Erythropoiesis
T10525 22491-22494 PR:Q6E2N3 denotes mon
T10526 22522-22527 PR:000016655 denotes tif1γ
T10527 22528-22538 GO:0010467 denotes expression
T10528 22542-22557 CL:0000764 denotes erythroid cells
T10529 22618-22622 SO:0000704 denotes gene
T10530 22618-22633 GO:0010467 denotes gene expression
T10531 22637-22650 GO:0030097 denotes hematopoietic
T10532 22637-22656 CL:0000988 denotes hematopoietic cells
T10533 22718-22723 UBERON:0007023 denotes adult
T10534 22724-22733 NCBITaxon:7955 denotes zebrafish
T10535 22734-22747 UBERON:0007132 denotes kidney marrow
T10536 22765-22770 PR:000007857 denotes gata1
T10537 22803-22812 SO:0000902 denotes transgene
T10538 22825-22828 PR:Q6E2N3 denotes mon
T10539 22836-22843 UBERON:0000922 denotes embryos
T10540 22870-22875 PR:000007857 denotes gata1
T10541 22880-22889 SO:0000902 denotes transgene
T10542 22907-22912 PR:000007857 denotes gata1
T10543 22913-22921 SO:0000167 denotes promoter
T10544 22935-22945 GO:0010467 denotes expression
T10545 22989-23004 CL:0000764 denotes erythroid cells
T10546 23047-23054 UBERON:0000922 denotes embryos
T10547 23063-23072 UBERON:0000922 denotes embryonic
T10548 23063-23072 _FRAGMENT denotes embryonic
T10549 23079-23084 CL:0002321 denotes cells
T10550 23073-23078 UBERON:0000178 denotes blood
T10551 23073-23084 CL:0000081 denotes blood cells
T10552 23079-23099 CL:0000080 denotes cells in circulation
T10553 23135-23139 GO:0018995 denotes host
T10554 23140-23147 UBERON:0000922 denotes embryos
T10555 23192-23199 UBERON:0000922 denotes embryos
T10556 23232-23252 CL:0000080 denotes cells in circulation
T10557 23502-23517 CL:0000764 denotes erythroid cells
T10558 23538-23558 CL:0000080 denotes cells in circulation
T10559 23600-23607 UBERON:0000922 denotes embryos
T10560 23635-23655 CL:0000080 denotes cells in circulation
T10561 23682-23687 UBERON:0000178 denotes blood
T10562 23682-23693 CL:0000081 denotes blood cells
T10563 23704-23711 UBERON:0000922 denotes embryos
T10564 23746-23751 UBERON:0000178 denotes blood
T10569 23950-23953 PR:Q6E2N3 denotes mon
T10570 23989-23998 UBERON:0000113 denotes adulthood
T10571 24068-24073 PR:000007857 denotes gata1
T10572 24167-24172 PR:000016655 denotes tif1γ
T10573 24205-24220 CL:0000764 denotes erythroid cells
T10574 24241-24254 GO:0030097 denotes hematopoietic
T10575 24255-24262 UBERON:0000479 denotes tissues
T10576 24272-24288 UBERON:0005256 denotes trunk mesenchyme
T10577 24296-24310 UBERON:0001016 denotes nervous system
T10578 24333-24339 UBERON:0000922 denotes embryo
T11802 25574-25579 PR:000016652 denotes TIF1β
T11780 24924-24929 PR:000016655 denotes Tif1γ
T11781 24942-24949 GO:0005634 denotes Nuclear
T11782 24974-24983 GO:0065007 denotes Regulated
T11783 25036-25041 PR:000016655 denotes Tif1γ
T11784 25085-25091 NCBITaxon:9986 denotes rabbit
T11785 25107-25112 UBERON:0001977 denotes serum
T11786 25173-25178 NCBITaxon:9606 denotes human
T11787 25179-25184 PR:000016655 denotes TIF1γ
T11788 25189-25194 NCBITaxon:10088 denotes mouse
T11789 25195-25200 PR:000016655 denotes Tif1γ
T11790 25224-25229 NCBITaxon:10088 denotes mouse
T11791 25230-25236 UBERON:0000922 denotes embryo
T11792 25237-25247 CL:0000057 denotes fibroblast
T11793 25248-25254 GO:0005634 denotes nuclei
T11794 25269-25274 PR:000016655 denotes Tif1γ
T11795 25279-25284 UBERON:0001977 denotes serum
T11796 25303-25308 PR:000016655 denotes Tif1γ
T11797 25331-25338 GO:0005634 denotes nuclear
T11798 25377-25382 PR:000016655 denotes Tif1γ
T11799 25431-25438 GO:0005634 denotes nuclear
T11800 25477-25482 PR:000016649 denotes Tif1α
T11801 25512-25517 PR:000016652 denotes TIF1β
T11803 25596-25611 GO:0000792 denotes heterochromatin
T11804 25634-25647 CHEBI:26536 denotes retinoic acid
T11805 25661-25666 UBERON:0001977 denotes serum
T11806 25722-25732 GO:0010467 denotes expression
T11807 25761-25770 GO:0065007 denotes regulated
T11808 25841-25848 GO:0005634 denotes nuclear
T11809 25867-25872 PR:000016655 denotes Tif1γ
T11810 25911-25926 GO:0000792 denotes heterochromatin
T11811 25928-25932 PR:000005086 denotes HP1α
T11812 25945-25949 CHEBI:51231 denotes DAPI
T11813 25992-25997 PR:000016655 denotes Tif1γ
T11814 26023-26036 CL:0000836 denotes promyelocytic
T11815 26023-26045 PR:000026474 denotes promyelocytic leukemia
T11816 26023-26058 _FRAGMENT denotes promyelocytic leukemia gene product
T11817 26065-26079 GO:0016605 denotes nuclear bodies
T11818 26046-26050 SO:0000704 denotes gene
T11819 26060-26063 PR:000026474 denotes PML
T11820 26060-26063 _FRAGMENT denotes PML
T11821 26081-26091 GO:0006281 denotes DNA repair
T11822 26081-26101 GO:1990391 denotes DNA repair complexes
T11823 26115-26120 PR:000010562 denotes Mre11
T11824 26141-26150 GO:0032991 denotes complexes
T11825 26162-26168 PR:000008313 denotes TFII-B
T11826 26210-26220 GO:0010467 denotes expression
T11827 26224-26229 PR:000016655 denotes Tif1γ
T11828 26249-26267 _FRAGMENT denotes differentiation of
T11829 26272-26277 GO:0030154 denotes cells
T11830 26281-26287 NCBITaxon:39107 denotes murine
T11831 26353-26365 CL:0000232 denotes erythrocytes
T11832 26373-26378 PR:000007857 denotes Gata1
T11833 26379-26387 CHEBI:50114 denotes estrogen
T11834 26441-26449 CHEBI:50114 denotes estrogen
T11835 26520-26525 PR:000016655 denotes Tif1γ
T11836 26534-26544 GO:0010467 denotes expression
T11837 26569-26578 CL:0000764 denotes erythroid
T11838 26652-26661 NCBITaxon:7955 denotes zebrafish
T11839 26662-26665 PR:Q6E2N3 denotes mon
T11840 26671-26681 GO:0010467 denotes expression
T11841 26708-26721 _FRAGMENT denotes maturation of
T11842 26746-26751 GO:0048469 denotes cells
T11843 26736-26751 CL:0000764 denotes erythroid cells
T11844 26789-26795 NCBITaxon:39107 denotes murine
T11845 26838-26843 PR:000016655 denotes Tif1γ
T11846 26852-26861 GO:0010467 denotes expressed
T11847 26865-26872 GO:0005634 denotes nuclear
T11848 26907-26912 PR:000016655 denotes Tif1γ
T11849 26944-26951 GO:0005634 denotes nuclear
T11850 27081-27086 PR:000016655 denotes Tif1γ
T11851 27105-27112 GO:0005634 denotes nuclear
T11852 27126-27135 CL:0000764 denotes erythroid
T13407 28947-28958 SO:0000858 denotes orthologous
T12510 4-13 NCBITaxon:7955 denotes Zebrafish
T12511 27975-27986 GO:0065007 denotes controlling
T12512 27987-27991 SO:0000704 denotes gene
T12513 27987-28002 GO:0010467 denotes gene expression
T12514 28010-28023 GO:0030097 denotes hematopoiesis
T12515 28103-28110 SO:0000704 denotes genetic
T12516 28190-28197 SO:0001023 denotes alleles
T12517 28205-28214 NCBITaxon:7955 denotes zebrafish
T12518 28215-28219 SO:0000704 denotes gene
T12519 28234-28243 PR:Q6E2N3 denotes moonshine
T12520 28310-28313 PR:Q6E2N3 denotes mon
T12521 28314-28318 SO:0000704 denotes gene
T12522 28392-28397 PR:000016655 denotes Tif1γ
T12523 28406-28419 GO:0030097 denotes hematopoietic
T13408 28959-28964 NCBITaxon:9606 denotes human
T13409 28969-28974 NCBITaxon:10088 denotes mouse
T13410 28982-28991 NCBITaxon:7955 denotes Zebrafish
T13411 28992-28997 PR:000016655 denotes tif1γ
T13412 29024-29033 NCBITaxon:7955 denotes zebrafish
T13413 29047-29055 SO:0000860 denotes syntenic
T13386 28438-28441 PR:Q6E2N3 denotes mon
T13387 28442-28446 SO:0000704 denotes Gene
T13388 28459-28468 NCBITaxon:7955 denotes Zebrafish
T13389 28469-28477 SO:0000855 denotes Ortholog
T13390 28481-28490 NCBITaxon:40674 denotes Mammalian
T13391 28491-28496 PR:000016655 denotes TIF1γ
T13392 28578-28587 NCBITaxon:7955 denotes zebrafish
T13393 28588-28591 PR:Q6E2N3 denotes mon
T13394 28592-28596 SO:0000704 denotes gene
T13395 28622-28630 SO:0000855 denotes ortholog
T13396 28634-28643 NCBITaxon:40674 denotes mammalian
T13397 28644-28649 PR:000016655 denotes Tif1γ
T13398 28651-28656 PR:000016655 denotes Tif1γ
T13399 28684-28691 SO:0000704 denotes genetic
T13400 28743-28746 SO:0000154 denotes PAC
T13401 28830-28839 NCBITaxon:7955 denotes zebrafish
T13402 28840-28845 PR:000016655 denotes tif1γ
T13403 28899-28905 SO:0000188 denotes intron
T13404 28906-28910 SO:0000147 denotes exon
T13405 28937-28946 _FRAGMENT denotes predicted
T13406 28975-28980 SO:0000996 denotes genes
T13414 29073-29078 NCBITaxon:9606 denotes human
T13415 29103-29108 PR:000016655 denotes TIF1γ
T13416 29143-29148 PR:000016655 denotes tif1γ
T13417 29170-29177 SO:0001023 denotes alleles
T13418 29181-29184 PR:Q6E2N3 denotes mon
T13419 29205-29226 SO:0001587 denotes premature stop codons
T13420 29231-29241 GO:0006402 denotes mRNA decay
T13421 29256-29261 PR:000016655 denotes tif1γ
T13422 29262-29267 PR:000016655 denotes Tif1γ
T13423 29278-29287 GO:0010467 denotes expressed
T13424 29291-29304 GO:0030097 denotes hematopoietic
T13425 29291-29310 CL:0000988 denotes hematopoietic cells
T13426 29322-29335 GO:0009790 denotes embryogenesis
T13427 29344-29353 NCBITaxon:7955 denotes zebrafish
T13428 29358-29363 NCBITaxon:10088 denotes mouse
T13429 29405-29415 GO:0010467 denotes expression
T13430 29429-29434 PR:000016655 denotes tif1γ
T13431 29460-29473 GO:0030097 denotes hematopoiesis
T13432 29477-29480 PR:Q6E2N3 denotes mon
T13433 29510-29526 _FRAGMENT denotes hematopoiesis in
T13434 29537-29544 GO:0035162 denotes embryos
T13435 29537-29544 UBERON:0000922 denotes embryos
T13436 29595-29604 NCBITaxon:7955 denotes zebrafish
T13437 29605-29613 SO:0000855 denotes ortholog
T13438 29617-29622 PR:000016649 denotes tif1α
T13439 29648-29657 GO:0010467 denotes expressed
T13440 29661-29670 NCBITaxon:7955 denotes zebrafish
T13441 29671-29678 UBERON:0000922 denotes embryos
T13442 29684-29693 NCBITaxon:40674 denotes mammalian
T13443 29694-29699 PR:000016649 denotes TIF1α
T13444 29837-29842 PR:000016655 denotes Tif1γ
T13445 29865-29878 GO:0030097 denotes hematopoietic
T13446 29865-29884 CL:0000988 denotes hematopoietic cells
T13447 29906-29915 NCBITaxon:7955 denotes zebrafish
T13448 29920-29929 NCBITaxon:40674 denotes mammalian
T13449 29987-29992 PR:000016655 denotes Tif1γ
T13450 29993-30002 SO:0000855 denotes orthologs
T13451 30058-30063 PR:000016649 denotes Tif1α
T13452 30125-30130 PR:000016652 denotes Tif1β
T13453 30131-30139 SO:0000855 denotes ortholog
T13454 30158-30167 NCBITaxon:7955 denotes zebrafish
T13455 30171-30175 NCBITaxon:31032 denotes fugu
T13456 30176-30182 SO:0001026 denotes genome
T13457 30186-30189 SO:0000345 denotes EST
T13458 30221-30226 PR:000016652 denotes Tif1β
T13459 30242-30248 SO:0000417 denotes domain
T13460 30307-30316 NCBITaxon:32523 denotes tetrapods
T13461 30356-30362 SO:0001026 denotes genome
T13462 30441-30450 NCBITaxon:7955 denotes zebrafish
T13463 30451-30454 PR:Q6E2N3 denotes mon
T13464 30497-30502 PR:000016655 denotes Tif1γ
T13465 30594-30599 NCBITaxon:9606 denotes human
T13466 30604-30609 NCBITaxon:10088 denotes mouse
T13467 30610-30623 GO:0030097 denotes hematopoiesis
T15026 31461-31470 UBERON:0000922 denotes embryonic
T15027 31471-31484 UBERON:0003061 denotes blood islands
T15028 31538-31551 GO:0030097 denotes hematopoietic
T15029 31538-31557 CL:0000988 denotes hematopoietic cells
T15030 31601-31610 CL:0000764 denotes erythroid
T15031 31652-31667 CL:0000764 denotes erythroid cells
T15032 31680-31689 GO:0006915 denotes apoptosis
T14998 30639-30644 PR:000016655 denotes tif1γ
T14999 30651-30660 GO:0006915 denotes Apoptosis
T15000 30664-30673 CL:0000764 denotes Erythroid
T15001 30705-30718 GO:0030097 denotes hematopoietic
T15002 30719-30723 SO:0000704 denotes gene
T15003 30719-30734 GO:0010467 denotes gene expression
T15004 30736-30745 GO:0006915 denotes apoptosis
T15005 30771-30774 PR:Q6E2N3 denotes mon
T15006 30807-30816 CL:0000764 denotes erythroid
T15007 30923-30944 GO:0012501 denotes programmed cell death
T15008 30965-30975 GO:0010467 denotes expression
T15009 30979-30984 PR:000007857 denotes gata1
T15010 31031-31046 CL:0000764 denotes erythroid cells
T15011 31050-31053 PR:Q6E2N3 denotes mon
T15012 31125-31130 PR:000007857 denotes gata1
T15013 31131-31141 GO:0010467 denotes expression
T15014 31158-31173 CL:0000445 denotes apoptotic cells
T15015 31197-31203 UBERON:0002329 denotes somite
T15016 31244-31257 GO:0030097 denotes hematopoietic
T15017 31258-31262 SO:0000704 denotes gene
T15018 31258-31273 GO:0010467 denotes gene expression
T15019 31295-31305 GO:0010467 denotes expression
T15020 31316-31321 SO:0000704 denotes genes
T15021 31333-31336 PR:000016043 denotes scl
T15022 31341-31346 PR:000007858 denotes gata2
T15023 31366-31379 GO:0030097 denotes hematopoietic
T15024 31366-31390 CL:0000037 denotes hematopoietic stem cells
T15025 31395-31418 GO:0060215 denotes primitive hematopoietic
T15033 31702-31707 PR:000007857 denotes gata1
T15034 31718-31733 CL:0000764 denotes erythroid cells
T15035 31787-31801 GO:0030218 denotes erythropoiesis
T15036 31875-31878 PR:Q6E2N3 denotes mon
T15037 31879-31885 UBERON:0007023 denotes adults
T15038 31906-31911 PR:000016655 denotes tif1γ
T15039 31932-31956 GO:0060216 denotes definitive hematopoiesis
T15040 31977-31984 CL:0000365 denotes zygotic
T15041 31999-32002 PR:Q6E2N3 denotes mon
T15042 32063-32068 PR:000016655 denotes Tif1γ
T15043 32081-32090 GO:0010467 denotes expressed
T15044 32091-32100 NCBITaxon:7955 denotes zebrafish
T15045 32101-32106 PR:000016655 denotes Tif1γ
T15046 32125-32138 GO:0030097 denotes hematopoiesis
T15047 32159-32172 GO:0048513 denotes organogenesis
T15048 32238-32242 CL:0000025 denotes eggs
T15049 32313-32316 PR:Q6E2N3 denotes mon
T15050 32331-32340 NCBITaxon:7955 denotes zebrafish
T15051 32422-32429 CL:0000365 denotes zygotic
T15052 32430-32433 PR:Q6E2N3 denotes mon
T15053 32501-32506 PR:000016655 denotes tif1γ
T15054 32524-32538 GO:0030218 denotes erythropoiesis
T15055 32588-32601 GO:0030097 denotes hematopoietic
T15056 32616-32629 GO:0030097 denotes hematopoietic
T15057 32643-32646 PR:Q6E2N3 denotes mon
T15058 32677-32692 CL:0000764 denotes erythroid cells
T15059 32706-32711 NCBITaxon:10088 denotes mouse
T15060 32712-32717 PR:000007857 denotes Gata1
T15061 32727-32734 UBERON:0000922 denotes embryos
T15062 32739-32748 NCBITaxon:7955 denotes zebrafish
T15063 32761-32766 PR:000007857 denotes gata1
T15064 32775-32782 UBERON:0000922 denotes embryos
T15065 32866-32873 SO:0000704 denotes genetic
T15066 32895-32898 PR:Q6E2N3 denotes mon
T15067 32903-32908 PR:000007857 denotes gata1
T15068 32944-32958 GO:0030218 denotes erythropoiesis
T15069 32978-32983 PR:000007857 denotes gata1
T15070 32994-32997 PR:Q6E2N3 denotes mon
T15071 33016-33023 UBERON:0000922 denotes embryos
T15072 33041-33046 PR:000016655 denotes tif1γ
T15073 33057-33062 PR:000007857 denotes gata1
T15074 33074-33081 UBERON:0000922 denotes embryos
T15075 33099-33112 GO:0030097 denotes hematopoiesis
T15076 33181-33186 PR:000016655 denotes Tif1γ
T15077 33191-33196 PR:000007857 denotes Gata1
T15078 33344-33349 SO:0000704 denotes genes
T15079 33423-33428 PR:000007857 denotes gata1
T15080 33433-33438 PR:000016655 denotes tif1γ
T15081 33511-33518 SO:0000704 denotes genetic
T15082 33538-33543 PR:000007857 denotes gata1
T15083 33548-33553 PR:000016655 denotes tif1γ
T15084 33575-33583 GO:0065007 denotes regulate
T15085 33584-33588 SO:0000704 denotes gene
T15086 33610-33615 UBERON:0000178 denotes blood
T15087 33610-33621 CL:0000081 denotes blood cells
T16271 33636-33641 PR:000016655 denotes Tif1γ
T16272 33645-33654 _FRAGMENT denotes Primitive
T16273 33670-33684 GO:0060319 denotes Erythropoiesis
T16274 33659-33684 GO:0060318 denotes Definitive Erythropoiesis
T16275 33723-33728 PR:000016655 denotes tif1γ
T16276 33757-33765 CHEBI:23357 denotes cofactor
T16277 33774-33783 CL:0000764 denotes erythroid
T16278 33801-33811 _FRAGMENT denotes control of
T16279 33826-33841 GO:0010468 denotes gene expression
T16280 33812-33825 GO:0030097 denotes hematopoietic
T16281 33826-33830 SO:0000704 denotes gene
T16282 33870-33875 PR:000016655 denotes Tif1γ
T16283 33938-33951 GO:0030097 denotes hematopoietic
T16284 33938-33968 CL:0008001 denotes hematopoietic progenitor cells
T16285 33985-33990 PR:000016649 denotes TIF1α
T16286 34015-34020 PR:000016652 denotes TIF1β
T16287 34150-34154 SO:0000704 denotes gene
T16288 34198-34203 PR:000016649 denotes TIF1α
T16289 34208-34213 PR:000016652 denotes TIF1β
T16290 34287-34296 GO:0000785 denotes chromatin
T16291 34329-34339 GO:0065007 denotes regulatory
T16292 34340-34349 GO:0032991 denotes complexes
T16293 34351-34356 PR:000016655 denotes Tif1γ
T16294 34376-34383 GO:0005634 denotes nuclear
T16295 34504-34518 GO:0016604 denotes nuclear bodies
T16296 34530-34533 PR:000026474 denotes PML
T16297 34530-34540 GO:0016605 denotes PML bodies
T16298 34558-34563 PR:000016655 denotes Tif1γ
T16299 34573-34587 GO:0016604 denotes nuclear bodies
T16300 34595-34604 GO:0065007 denotes regulated
T16301 34612-34625 GO:0006412 denotes translational
T16302 34686-34689 PR:000026474 denotes PML
T16303 34698-34701 PR:000026474 denotes PML
T16304 34698-34717 GO:0016605 denotes PML nuclear domains
T16305 34811-34816 PR:000016655 denotes Tif1γ
T16306 34836-34845 GO:0032991 denotes complexes
T16307 34968-34977 CL:0000764 denotes erythroid
T16308 35045-35050 PR:000016655 denotes Tif1γ
T16309 35075-35082 GO:0005634 denotes nuclear
T16310 35125-35130 PR:000016655 denotes Tif1γ
T16311 35134-35142 GO:0065007 denotes regulate
T16312 35143-35148 UBERON:0000178 denotes blood
T16313 35143-35153 CL:0000081 denotes blood cell
T16314 35149-35165 GO:0048468 denotes cell development
T16989 35305-35312 SO:0001023 denotes alleles
T16990 35313-35316 PR:Q6E2N3 denotes mon
T16986 35191-35200 NCBITaxon:7955 denotes Zebrafish
T16987 35205-35210 NCBITaxon:10088 denotes mouse
T16988 35231-35240 NCBITaxon:7955 denotes Zebrafish
T16991 35327-35330 PR:Q6E2N3 denotes mon
T16992 35379-35382 CHEBI:23995 denotes ENU
T16993 35445-35448 PR:Q6E2N3 denotes mon
T16994 35453-35459 SO:0001023 denotes allele
T16995 35584-35590 GO:0007618 denotes mating
T16996 35679-35686 UBERON:0000922 denotes embryos
T16997 35735-35748 GO:0097617 denotes hybridization
T16998 35766-35773 UBERON:0000922 denotes embryos
T16999 35847-35860 GO:0097617 denotes hybridization
T17000 35864-35869 NCBITaxon:10088 denotes mouse
T17001 35870-35877 UBERON:0000922 denotes embryos
T17002 35929-35936 SO:0001026 denotes Genomic
T17003 35964-35968 SO:0001830 denotes AFLP
T17004 36111-36118 SO:0000112 denotes primers
T17005 36123-36130 SO:0000704 denotes genetic
T17006 36166-36169 PR:Q6E2N3 denotes mon
T17322 36202-36212 GO:0010467 denotes expression
T17323 36225-36236 SO:0001183 denotes morpholinos
T17324 36273-36276 PR:Q6E2N3 denotes mon
T17325 36335-36341 SO:0000440 denotes vector
T17613 36683-36686 PR:Q6E2N3 denotes mon
T17614 36708-36715 UBERON:0000922 denotes embryos
T17615 36737-36750 UBERON:0007132 denotes kidney marrow
T17616 36775-36781 UBERON:0000922 denotes embryo
T17617 36807-36814 UBERON:0000922 denotes embryos
T17607 36490-36503 UBERON:0007132 denotes kidney marrow
T17608 36529-36534 UBERON:0007023 denotes adult
T17609 36535-36540 PR:000007857 denotes gata1
T17610 36622-36628 NCBITaxon:27592 denotes bovine
T17611 36629-36634 UBERON:0001977 denotes serum
T17612 36658-36671 UBERON:0002063 denotes sinus venosus
T17618 37006-37012 UBERON:0002548 denotes larvae
T18138 37076-37086 GO:0042571 denotes Antibodies
T18139 37124-37128 UBERON:0001977 denotes sera
T18140 37141-37146 NCBITaxon:9606 denotes human
T18141 37158-37163 PR:000016655 denotes TIF1γ
T18142 37206-37213 NCBITaxon:9986 denotes rabbits
T18143 37305-37310 NCBITaxon:10088 denotes Mouse
T18144 37311-37320 UBERON:0000922 denotes embryonic
T18145 37321-37332 CL:0000057 denotes fibroblasts
T18146 37333-37338 GO:0040007 denotes grown
T18147 37377-37381 PR:000005086 denotes HP1α
T18148 37434-37439 PR:000016655 denotes Tif1γ
T18149 37444-37448 UBERON:0001977 denotes sera
T18150 37585-37590 UBERON:0001977 denotes serum
T18151 37644-37654 GO:0042571 denotes antibodies
T18152 37739-37749 GO:0042571 denotes antibodies
T18153 37876-37880 CHEBI:51231 denotes DAPI
T18154 38071-38091 GO:0030154 denotes cell differentiation
T550 1166-1176 GO:0010467 denotes expression
T7542 18649-18657 SO:0000860 denotes syntenic
T7543 18675-18680 NCBITaxon:9606 denotes human
T7544 18712-18717 PR:000016649 denotes TIF1α
T7545 18718-18722 SO:0000704 denotes gene
T7546 18771-18782 SO:0000858 denotes orthologous
T7547 18783-18787 SO:0000704 denotes gene
T7548 18805-18811 PR:000014615 denotes SEMA3A
T7549 18840-18845 NCBITaxon:9606 denotes human
T7550 18850-18859 NCBITaxon:7955 denotes zebrafish
T7551 18905-18914 UBERON:0000922 denotes embryonic
T7552 18915-18925 GO:0010467 denotes expression
T7553 18937-18942 PR:000016649 denotes tif1α
T7554 18951-18956 PR:000016655 denotes tif1γ
T7555 18968-18981 GO:0097617 denotes hybridization
T7556 18988-18997 NCBITaxon:40674 denotes mammalian
T7557 18998-19003 PR:000016649 denotes TIF1α
T7558 19061-19070 _FRAGMENT denotes predicted
T7559 19087-19091 SO:0000996 denotes gene
T7560 19071-19080 NCBITaxon:7955 denotes zebrafish
T7561 19081-19086 PR:000016655 denotes tif1γ
T7562 19103-19112 GO:0010467 denotes expressed
T7563 19138-19145 UBERON:0002329 denotes somites
T7564 19147-19156 NCBITaxon:7955 denotes zebrafish
T7565 19157-19162 PR:000016649 denotes tif1α
T7566 19200-19210 GO:0010467 denotes expression
T7567 19245-19258 GO:0030097 denotes hematopoietic
T7568 19259-19267 UBERON:0000926 denotes mesoderm
T7569 19278-19283 PR:000016655 denotes tif1γ
T7570 19302-19307 PR:000016649 denotes tif1α
T7571 19318-19327 GO:0010467 denotes expressed
T7572 19351-19357 UBERON:0000922 denotes embryo
T7573 19384-19397 UBERON:0003061 denotes blood islands
T7574 19410-19415 PR:000016649 denotes tif1α
T7575 19421-19430 GO:0010467 denotes expressed
T7576 19454-19459 PR:000016655 denotes tif1γ
R236 T2380 T2379 _lexicallyChainedTo cells,circulating
R237 T2382 T2381 _lexicallyChainedTo blood cells,red
R238 T2390 T2389 _lexicallyChainedTo cells,stem
R239 T2437 T2436 _lexicallyChainedTo hematopoiesis,primitive embryonic
R240 T2440 T2439 _lexicallyChainedTo hematopoiesis,definitive
R241 T2490 T2489 _lexicallyChainedTo hematopoiesis,embryonic
R999 T4407 T4406 _lexicallyChainedTo Erythropoiesis,Primitive
R1000 T4448 T4447 _lexicallyChainedTo embryos,development of
R1001 T4471 T4470 _lexicallyChainedTo of,development
R1002 T4472 T4471 _lexicallyChainedTo cells,of
R1003 T4473 T4471 _lexicallyChainedTo mesenchymal cells,of
R1004 T4499 T4498 _lexicallyChainedTo cells,differentiation of
R1005 T4504 T4503 _lexicallyChainedTo erythropoiesis,primitive
R1481 T7376 T7375 _lexicallyChainedTo gene,predicted
R1482 T7419 T7418 _lexicallyChainedTo cells,circulating
R1483 T7425 T7424 _lexicallyChainedTo cells,circulating
R1484 T7469 T7468 _lexicallyChainedTo mesoderm,ventral
R1485 T7487 T7486 _lexicallyChainedTo trunk,mesenchyme of
R1486 T7513 T7512 _lexicallyChainedTo yolk sac,blood islands of
R1487 T7559 T7558 _lexicallyChainedTo gene,predicted
R2625 T9540 T9539 _lexicallyChainedTo erythrocytes,formation of
R2626 T9546 T9545 _lexicallyChainedTo organogenesis,embryonic
R2845 T10549 T10548 _lexicallyChainedTo cells,embryonic
R3190 T11817 T11816 _lexicallyChainedTo nuclear bodies,promyelocytic leukemia gene product
R3191 T11817 T11820 _lexicallyChainedTo nuclear bodies,PML
R3192 T11829 T11828 _lexicallyChainedTo cells,differentiation of
R3193 T11842 T11841 _lexicallyChainedTo cells,maturation of
R3645 T13406 T13405 _lexicallyChainedTo genes,predicted
R3646 T13434 T13433 _lexicallyChainedTo embryos,hematopoiesis in
R4510 T16273 T16272 _lexicallyChainedTo Erythropoiesis,Primitive
R4511 T16279 T16278 _lexicallyChainedTo gene expression,control of
R1 T515 T514 _lexicallyChainedTo hematopoiesis,embryonic
R2 T548 T547 _lexicallyChainedTo formation,cell
R3 T561 T560 _lexicallyChainedTo cells,differentiation of
R235 T2378 T2377 _lexicallyChainedTo cells,differentiation of

craft-sa-dev

Id Subject Object Predicate Lexical cue
T8281 13841-13850 NN denotes zebrafish
T8282 13837-13840 CC denotes and
T8283 13851-13852 -LRB- denotes (
T8284 13852-13860 NNP denotes Barbazuk
T8285 13861-13863 FW denotes et
T8286 13864-13867 FW denotes al.
T8287 13868-13872 CD denotes 2000
T8288 13872-13873 -RRB- denotes )
T8289 13873-13874 . denotes .
T8290 13874-14011 sentence denotes Therefore, based on sequence similarity and chromosomal location, the zebrafish mon gene is the likely ortholog of the human TIF1γ gene.
T8291 13875-13884 RB denotes Therefore
T8292 13964-13966 VBZ denotes is
T8293 13884-13886 , denotes ,
T8294 13886-13891 VBN denotes based
T660 0-3 DT denotes The
T661 24-28 NN denotes Gene
T662 0-116 sentence denotes The Zebrafish moonshine Gene Encodes Transcriptional Intermediary Factor 1γ, an Essential Regulator of Hematopoiesis
T663 4-13 NN denotes Zebrafish
T664 14-23 NN denotes moonshine
T665 29-36 VBZ denotes Encodes
T666 37-52 JJ denotes Transcriptional
T667 73-75 NN denotes 1γ
T668 53-65 JJ denotes Intermediary
T669 66-72 NN denotes Factor
T670 75-77 , denotes ,
T671 77-79 DT denotes an
T672 90-99 NN denotes Regulator
T673 80-89 JJ denotes Essential
T674 100-102 IN denotes of
T675 103-116 NN denotes Hematopoiesis
T772 725-727 TO denotes to
T773 728-735 VB denotes express
T774 736-742 JJ denotes normal
T775 743-749 NNS denotes levels
T776 750-752 IN denotes of
T777 753-766 JJ denotes hematopoietic
T778 781-788 NNS denotes factors
T779 767-780 NN denotes transcription
T780 788-790 , denotes ,
T781 790-799 VBG denotes including
T782 800-805 NN denotes gata1
T783 805-807 , denotes ,
T784 807-810 CC denotes and
T785 811-818 VBP denotes undergo
T786 819-828 NN denotes apoptosis
T787 828-829 . denotes .
T788 829-1006 sentence denotes Three different mon mutant alleles each encode premature stop codons, and enforced expression of wild-type tif1γ mRNA rescues embryonic hematopoiesis in homozygous mon mutants.
T789 830-835 CD denotes Three
T790 857-864 NNS denotes alleles
T791 836-845 JJ denotes different
T792 846-849 NN denotes mon
T793 850-856 JJ denotes mutant
T8295 13892-13894 IN denotes on
T794 870-876 VBP denotes encode
T795 865-869 DT denotes each
T796 877-886 JJ denotes premature
T8296 13895-13903 NN denotes sequence
T797 892-898 NNS denotes codons
T798 887-891 NN denotes stop
T799 898-900 , denotes ,
T800 900-903 CC denotes and
T801 904-912 JJ denotes enforced
T802 913-923 NN denotes expression
T803 948-955 VBZ denotes rescues
T804 924-926 IN denotes of
T805 927-931 JJ denotes wild
T8297 13904-13914 NN denotes similarity
T678 116-315 sentence denotes moonshine/tif1γ in Hematopoiesis Abstract Hematopoiesis is precisely orchestrated by lineage-specific DNA-binding proteins that regulate transcription in concert with coactivators and corepressors.
T679 160-173 NN denotes Hematopoiesis
T680 187-199 VBN denotes orchestrated
T681 174-176 VBZ denotes is
T682 177-186 RB denotes precisely
T683 200-202 IN denotes by
T684 203-210 NN denotes lineage
T685 211-219 JJ denotes specific
T686 210-211 HYPH denotes -
T687 232-240 NN denotes proteins
T688 220-223 NN denotes DNA
T689 224-231 VBG denotes binding
T690 223-224 HYPH denotes -
T691 241-245 WDT denotes that
T692 246-254 VBP denotes regulate
T693 255-268 NN denotes transcription
T694 269-271 IN denotes in
T695 272-279 NN denotes concert
T696 280-284 IN denotes with
T697 285-297 NNS denotes coactivators
T698 298-301 CC denotes and
T699 302-314 NNS denotes corepressors
T700 314-315 . denotes .
T701 315-468 sentence denotes Mutations in the zebrafish moonshine (mon) gene specifically disrupt both embryonic and adult hematopoiesis, resulting in severe red blood cell aplasia.
T702 316-325 NNS denotes Mutations
T703 377-384 VBP denotes disrupt
T704 326-328 IN denotes in
T705 329-332 DT denotes the
T706 359-363 NN denotes gene
T707 333-342 NN denotes zebrafish
T708 343-352 NN denotes moonshine
T709 353-354 -LRB- denotes (
T710 354-357 NN denotes mon
T711 357-358 -RRB- denotes )
T712 364-376 RB denotes specifically
T713 385-389 CC denotes both
T714 390-399 JJ denotes embryonic
T715 410-423 NN denotes hematopoiesis
T716 400-403 CC denotes and
T717 404-409 JJ denotes adult
T718 423-425 , denotes ,
T719 425-434 VBG denotes resulting
T720 435-437 IN denotes in
T721 438-444 JJ denotes severe
T722 460-467 NN denotes aplasia
T723 445-448 JJ denotes red
T724 455-459 NN denotes cell
T725 449-454 NN denotes blood
T726 467-468 . denotes .
T727 468-653 sentence denotes We report that mon encodes the zebrafish ortholog of mammalian transcriptional intermediary factor 1γ (TIF1γ) (or TRIM33), a member of the TIF1 family of coactivators and corepressors.
T728 469-471 PRP denotes We
T729 472-478 VBP denotes report
T730 479-483 IN denotes that
T731 488-495 VBZ denotes encodes
T732 484-487 NN denotes mon
T733 496-499 DT denotes the
T734 510-518 NN denotes ortholog
T735 500-509 NN denotes zebrafish
T736 519-521 IN denotes of
T737 522-531 JJ denotes mammalian
T738 568-570 NN denotes 1γ
T739 532-547 JJ denotes transcriptional
T740 548-560 JJ denotes intermediary
T741 561-567 NN denotes factor
T742 571-572 -LRB- denotes (
T743 572-577 NN denotes TIF1γ
T744 577-578 -RRB- denotes )
T745 579-580 -LRB- denotes (
T746 580-582 CC denotes or
T747 583-589 NN denotes TRIM33
T748 589-590 -RRB- denotes )
T749 590-592 , denotes ,
T750 592-593 DT denotes a
T751 594-600 NN denotes member
T752 601-603 IN denotes of
T753 604-607 DT denotes the
T754 613-619 NN denotes family
T755 608-612 NN denotes TIF1
T756 620-622 IN denotes of
T757 623-635 NNS denotes coactivators
T758 636-639 CC denotes and
T759 640-652 NNS denotes corepressors
T760 652-653 . denotes .
T761 653-829 sentence denotes During development, hematopoietic progenitor cells in mon mutants fail to express normal levels of hematopoietic transcription factors, including gata1, and undergo apoptosis.
T762 654-660 IN denotes During
T763 720-724 VBP denotes fail
T764 661-672 NN denotes development
T765 672-674 , denotes ,
T766 674-687 JJ denotes hematopoietic
T767 699-704 NNS denotes cells
T768 688-698 NN denotes progenitor
T769 705-707 IN denotes in
T770 708-711 NN denotes mon
T771 712-719 NNS denotes mutants
T806 932-936 NN denotes type
T807 931-932 HYPH denotes -
T808 943-947 NN denotes mRNA
T809 937-942 NN denotes tif1γ
T810 956-965 JJ denotes embryonic
T811 966-979 NN denotes hematopoiesis
T812 980-982 IN denotes in
T813 983-993 JJ denotes homozygous
T814 998-1005 NNS denotes mutants
T815 994-997 NN denotes mon
T816 1005-1006 . denotes .
T817 1006-1177 sentence denotes Surprisingly, a high level of zygotic tif1γ mRNA expression delineates ventral mesoderm during hematopoietic stem cell and progenitor formation prior to gata1 expression.
T818 1007-1019 RB denotes Surprisingly
T819 1067-1077 VBZ denotes delineates
T820 1019-1021 , denotes ,
T821 1021-1022 DT denotes a
T822 1028-1033 NN denotes level
T823 1023-1027 JJ denotes high
T824 1034-1036 IN denotes of
T825 1037-1044 JJ denotes zygotic
T826 1045-1050 NN denotes tif1γ
T827 1051-1055 NN denotes mRNA
T828 1056-1066 NN denotes expression
T829 1078-1085 JJ denotes ventral
T830 1086-1094 NN denotes mesoderm
T831 1095-1101 IN denotes during
T832 1102-1115 JJ denotes hematopoietic
T833 1121-1125 NN denotes cell
T834 1116-1120 NN denotes stem
T835 1141-1150 NN denotes formation
T836 1126-1129 CC denotes and
T837 1130-1140 NN denotes progenitor
T838 1151-1156 IN denotes prior
T839 1157-1159 IN denotes to
T840 1160-1165 NN denotes gata1
T841 1166-1176 NN denotes expression
T842 1176-1177 . denotes .
T843 1177-1309 sentence denotes Transplantation studies reveal that tif1γ functions in a cell-autonomous manner during the differentiation of erythroid precursors.
T844 1178-1193 NN denotes Transplantation
T845 1194-1201 NNS denotes studies
T846 1202-1208 VBP denotes reveal
T847 1209-1213 IN denotes that
T848 1220-1229 VBZ denotes functions
T849 1214-1219 NN denotes tif1γ
T850 1230-1232 IN denotes in
T851 1233-1234 DT denotes a
T852 1251-1257 NN denotes manner
T853 1235-1239 NN denotes cell
T854 1240-1250 JJ denotes autonomous
T855 1239-1240 HYPH denotes -
T856 1258-1264 IN denotes during
T857 1265-1268 DT denotes the
T858 1269-1284 NN denotes differentiation
T859 1285-1287 IN denotes of
T860 1288-1297 JJ denotes erythroid
T861 1298-1308 NNS denotes precursors
T862 1308-1309 . denotes .
T863 1309-1478 sentence denotes Studies in murine erythroid cell lines demonstrate that Tif1γ protein is localized within novel nuclear foci, and expression decreases during erythroid cell maturation.
T864 1310-1317 NNS denotes Studies
T865 1349-1360 VBP denotes demonstrate
T866 1318-1320 IN denotes in
T867 1321-1327 JJ denotes murine
T868 1343-1348 NNS denotes lines
T869 1328-1337 JJ denotes erythroid
T870 1338-1342 NN denotes cell
T871 1361-1365 IN denotes that
T872 1383-1392 VBN denotes localized
T873 1366-1371 NN denotes Tif1γ
T874 1372-1379 NN denotes protein
T875 1380-1382 VBZ denotes is
T876 1393-1399 IN denotes within
T877 1400-1405 JJ denotes novel
T878 1414-1418 NNS denotes foci
T879 1406-1413 JJ denotes nuclear
T880 1418-1420 , denotes ,
T881 1420-1423 CC denotes and
T882 1424-1434 NN denotes expression
T883 1435-1444 VBZ denotes decreases
T884 1445-1451 IN denotes during
T885 1452-1461 JJ denotes erythroid
T886 1467-1477 NN denotes maturation
T887 1462-1466 NN denotes cell
T888 1477-1478 . denotes .
T889 1478-1620 sentence denotes Our results establish a major role for this transcriptional intermediary factor in the differentiation of hematopoietic cells in vertebrates.
T890 1479-1482 PRP$ denotes Our
T891 1483-1490 NNS denotes results
T892 1491-1500 VBP denotes establish
T893 1501-1502 DT denotes a
T894 1509-1513 NN denotes role
T895 1503-1508 JJ denotes major
T896 1514-1517 IN denotes for
T897 1518-1522 DT denotes this
T898 1552-1558 NN denotes factor
T899 1523-1538 JJ denotes transcriptional
T900 1539-1551 JJ denotes intermediary
T901 1559-1561 IN denotes in
T902 1562-1565 DT denotes the
T903 1566-1581 NN denotes differentiation
T904 1582-1584 IN denotes of
T905 1585-1598 JJ denotes hematopoietic
T906 1599-1604 NNS denotes cells
T907 1605-1607 IN denotes in
T908 1608-1619 NNS denotes vertebrates
T909 1619-1620 . denotes .
T8298 13915-13918 CC denotes and
T2707 1812-1825 NN denotes Hematopoiesis
T2708 1826-1834 VBZ denotes involves
T2709 1835-1838 DT denotes the
T2710 1851-1860 NNS denotes processes
T2711 1839-1850 VBN denotes coordinated
T2712 1861-1863 IN denotes of
T2713 1864-1868 NN denotes cell
T2714 1869-1882 NN denotes proliferation
T2715 1883-1886 CC denotes and
T2716 1887-1902 NN denotes differentiation
T2717 1903-1905 IN denotes of
T2718 1906-1907 DT denotes a
T2719 1925-1931 NN denotes number
T2720 1908-1918 RB denotes relatively
T2721 1919-1924 JJ denotes small
T2722 1932-1934 IN denotes of
T2723 1935-1945 NN denotes progenitor
T2724 1946-1951 NNS denotes cells
T2725 1952-1956 IN denotes into
T2726 1957-1965 NNS denotes billions
T2727 1966-1968 IN denotes of
T2728 1969-1980 VBG denotes circulating
T2729 2001-2006 NNS denotes cells
T2730 1981-1984 JJ denotes red
T2731 1985-1988 CC denotes and
T2732 1989-1994 JJ denotes white
T2733 1995-2000 NN denotes blood
T2734 2007-2008 -LRB- denotes (
T2735 2008-2014 NNP denotes Thisse
T2736 2015-2018 CC denotes and
T2737 2019-2022 NNP denotes Zon
T2738 2023-2027 CD denotes 2002
T2739 2027-2028 -RRB- denotes )
T2740 2028-2029 . denotes .
T2741 2029-2346 sentence denotes Hematopoiesis in vertebrates, from zebrafish to humans, is an evolutionarily conserved program that produces two waves of stem or progenitor cells that differ both in their embryonic origins and in the lineages of differentiated blood cells produced (Palis and Yoder 2001; Orkin and Zon 2002; Galloway and Zon 2003).
T2742 2030-2043 NN denotes Hematopoiesis
T2743 2086-2088 VBZ denotes is
T2744 2044-2046 IN denotes in
T2745 2047-2058 NNS denotes vertebrates
T2746 2058-2060 , denotes ,
T2747 2060-2064 IN denotes from
T2748 2065-2074 NN denotes zebrafish
T2749 2075-2077 IN denotes to
T2750 2078-2084 NNS denotes humans
T2751 2084-2086 , denotes ,
T2752 2089-2091 DT denotes an
T2753 2117-2124 NN denotes program
T2754 2092-2106 RB denotes evolutionarily
T2755 2107-2116 VBN denotes conserved
T2756 2125-2129 WDT denotes that
T2757 2130-2138 VBZ denotes produces
T2758 2139-2142 CD denotes two
T2759 2143-2148 NNS denotes waves
T2760 2149-2151 IN denotes of
T2761 2152-2156 NN denotes stem
T2762 2171-2176 NNS denotes cells
T2763 2157-2159 CC denotes or
T2764 2160-2170 NN denotes progenitor
T2765 2177-2181 WDT denotes that
T2766 2182-2188 VBP denotes differ
T2767 2189-2193 CC denotes both
T2768 2194-2196 IN denotes in
T2769 2197-2202 PRP$ denotes their
T2770 2213-2220 NNS denotes origins
T2771 2203-2212 JJ denotes embryonic
T2772 2221-2224 CC denotes and
T2773 2225-2227 IN denotes in
T2774 2228-2231 DT denotes the
T2775 2232-2240 NNS denotes lineages
T2776 2241-2243 IN denotes of
T2777 2244-2258 VBN denotes differentiated
T2778 2265-2270 NNS denotes cells
T2779 2259-2264 NN denotes blood
T2780 2271-2279 VBN denotes produced
T2781 2280-2281 -LRB- denotes (
T2782 2281-2286 NNP denotes Palis
T2783 2287-2290 CC denotes and
T2784 2291-2296 NNP denotes Yoder
T2785 2297-2301 CD denotes 2001
T2786 2301-2302 : denotes ;
T2787 2303-2308 NNP denotes Orkin
T2788 2309-2312 CC denotes and
T2789 2313-2316 NNP denotes Zon
T2790 2317-2321 CD denotes 2002
T2791 2321-2322 : denotes ;
T2792 2323-2331 NNP denotes Galloway
T2793 2332-2335 CC denotes and
T2794 2336-2339 NNP denotes Zon
T2795 2340-2344 CD denotes 2003
T2796 2344-2345 -RRB- denotes )
T2797 2345-2346 . denotes .
T2798 2346-2508 sentence denotes The first, or primitive, wave of hematopoiesis originates from ventral mesoderm and gives rise to progenitor cells that differentiate in embryonic blood islands.
T2799 2347-2350 DT denotes The
T2800 2372-2376 NN denotes wave
T2801 2351-2356 JJ denotes first
T2802 2356-2358 , denotes ,
T2803 2358-2360 CC denotes or
T2804 2361-2370 JJ denotes primitive
T2805 2370-2372 , denotes ,
T2806 2394-2404 VBZ denotes originates
T2807 2377-2379 IN denotes of
T2808 2380-2393 NN denotes hematopoiesis
T2809 2405-2409 IN denotes from
T2810 2410-2417 JJ denotes ventral
T2811 2418-2426 NN denotes mesoderm
T2812 2427-2430 CC denotes and
T2813 2431-2436 VBZ denotes gives
T2814 2437-2441 NN denotes rise
T2815 2442-2444 IN denotes to
T2816 2445-2455 NN denotes progenitor
T2817 2456-2461 NNS denotes cells
T2818 2462-2466 WDT denotes that
T2819 2467-2480 VBP denotes differentiate
T2820 2481-2483 IN denotes in
T2821 2484-2493 JJ denotes embryonic
T2822 2500-2507 NNS denotes islands
T2823 2494-2499 NN denotes blood
T2824 2507-2508 . denotes .
T2825 2508-2604 sentence denotes The primitive wave of hematopoiesis produces a burst of embryonic erythrocytes and macrophages.
T2826 2509-2512 DT denotes The
T2827 2523-2527 NN denotes wave
T2828 2513-2522 JJ denotes primitive
T2829 2545-2553 VBZ denotes produces
T2830 2528-2530 IN denotes of
T2831 2531-2544 NN denotes hematopoiesis
T2832 2554-2555 DT denotes a
T2833 2556-2561 NN denotes burst
T2834 2562-2564 IN denotes of
T2835 2565-2574 JJ denotes embryonic
T2836 2575-2587 NNS denotes erythrocytes
T2837 2588-2591 CC denotes and
T2838 2592-2603 NNS denotes macrophages
T2839 2603-2604 . denotes .
T2840 2604-2767 sentence denotes The second, or definitive, wave of hematopoiesis arises from self-renewing stem cells that develop primarily in the intraembryonic aorta–gonad–mesonephros region.
T2841 2605-2608 DT denotes The
T2842 2632-2636 NN denotes wave
T2843 2609-2615 JJ denotes second
T2844 2615-2617 , denotes ,
T2845 2617-2619 CC denotes or
T2846 2620-2630 JJ denotes definitive
T2847 2630-2632 , denotes ,
T2848 2654-2660 VBZ denotes arises
T2849 2637-2639 IN denotes of
T2850 2640-2653 NN denotes hematopoiesis
T2851 2661-2665 IN denotes from
T2852 2666-2670 NN denotes self
T2853 2671-2679 VBG denotes renewing
T2854 2670-2671 HYPH denotes -
T2855 2685-2690 NNS denotes cells
T2856 2680-2684 NN denotes stem
T2857 2691-2695 WDT denotes that
T2858 2696-2703 VBP denotes develop
T2859 2704-2713 RB denotes primarily
T2860 2714-2716 IN denotes in
T2861 2717-2720 DT denotes the
T2862 2760-2766 NN denotes region
T2863 2721-2735 JJ denotes intraembryonic
T2864 2736-2741 NN denotes aorta
T2865 2748-2759 NN denotes mesonephros
T2866 2741-2742 HYPH denotes –
T2867 2742-2747 NN denotes gonad
T2868 2747-2748 HYPH denotes –
T2869 2766-2767 . denotes .
T2870 2767-2963 sentence denotes These definitive hematopoietic stem cells seed the later developing marrow spaces, to produce all lineages of adult blood cells, including definitive erythrocytes, myeloid cells, and lymphocytes.
T2871 2768-2773 DT denotes These
T2872 2804-2809 NNS denotes cells
T2873 2774-2784 JJ denotes definitive
T2874 2785-2798 JJ denotes hematopoietic
T2875 2799-2803 NN denotes stem
T2876 2810-2814 VBP denotes seed
T2877 2815-2818 DT denotes the
T2878 2843-2849 NNS denotes spaces
T2879 2819-2824 RB denotes later
T2880 2825-2835 VBG denotes developing
T2881 2836-2842 NN denotes marrow
T2882 2849-2851 , denotes ,
T2883 2851-2853 TO denotes to
T2884 2854-2861 VB denotes produce
T2885 2862-2865 DT denotes all
T2886 2866-2874 NNS denotes lineages
T2887 2875-2877 IN denotes of
T2888 2878-2883 JJ denotes adult
T2889 2890-2895 NNS denotes cells
T2890 2884-2889 NN denotes blood
T2891 2895-2897 , denotes ,
T2892 2897-2906 VBG denotes including
T2893 2907-2917 JJ denotes definitive
T2894 2918-2930 NNS denotes erythrocytes
T2895 2930-2932 , denotes ,
T2896 2932-2939 JJ denotes myeloid
T2897 2940-2945 NNS denotes cells
T2898 2945-2947 , denotes ,
T2899 2947-2950 CC denotes and
T2900 2951-2962 NNS denotes lymphocytes
T2901 2962-2963 . denotes .
T2902 2963-3111 sentence denotes We have undertaken a genetic approach to characterize genes that control hematopoiesis using the zebrafish as a model system (Thisse and Zon 2002).
T2903 2964-2966 PRP denotes We
T2904 2972-2982 VBN denotes undertaken
T2905 2967-2971 VBP denotes have
T2906 2983-2984 DT denotes a
T2907 2993-3001 NN denotes approach
T2908 2985-2992 JJ denotes genetic
T2909 3002-3004 TO denotes to
T2910 3005-3017 VB denotes characterize
T2911 3018-3023 NNS denotes genes
T2912 3024-3028 WDT denotes that
T2913 3029-3036 VBP denotes control
T2914 3037-3050 NN denotes hematopoiesis
T2915 3051-3056 VBG denotes using
T2916 3057-3060 DT denotes the
T2917 3061-3070 NN denotes zebrafish
T2918 3071-3073 IN denotes as
T2919 3074-3075 DT denotes a
T2920 3082-3088 NN denotes system
T2921 3076-3081 NN denotes model
T2922 3089-3090 -LRB- denotes (
T2923 3090-3096 NNP denotes Thisse
T2924 3097-3100 CC denotes and
T2925 3101-3104 NNP denotes Zon
T2926 3105-3109 CD denotes 2002
T2927 3109-3110 -RRB- denotes )
T2928 3110-3111 . denotes .
T2929 3111-3346 sentence denotes As part of a large-scale forward genetic screen, we previously identified bloodless zebrafish mutants that failed to express the erythroid transcription factor gata1 normally in embryonic hematopoietic precursors (Ransom et al. 1996).
T2930 3112-3114 IN denotes As
T2931 3175-3185 VBD denotes identified
T2932 3115-3119 NN denotes part
T2933 3120-3122 IN denotes of
T2934 3123-3124 DT denotes a
T2935 3153-3159 NN denotes screen
T2936 3125-3130 JJ denotes large
T2937 3131-3136 NN denotes scale
T2938 3130-3131 HYPH denotes -
T2939 3137-3144 JJ denotes forward
T2940 3145-3152 JJ denotes genetic
T2941 3159-3161 , denotes ,
T2942 3161-3163 PRP denotes we
T2943 3164-3174 RB denotes previously
T2944 3186-3195 JJ denotes bloodless
T2945 3206-3213 NNS denotes mutants
T2946 3196-3205 NN denotes zebrafish
T2947 3214-3218 WDT denotes that
T2948 3219-3225 VBD denotes failed
T2949 3226-3228 TO denotes to
T2950 3229-3236 VB denotes express
T2951 3237-3240 DT denotes the
T2952 3272-3277 NN denotes gata1
T2953 3241-3250 JJ denotes erythroid
T2954 3251-3264 NN denotes transcription
T2955 3265-3271 NN denotes factor
T2956 3278-3286 RB denotes normally
T2957 3287-3289 IN denotes in
T2958 3290-3299 JJ denotes embryonic
T2959 3314-3324 NNS denotes precursors
T2960 3300-3313 JJ denotes hematopoietic
T2961 3325-3326 -LRB- denotes (
T2962 3326-3332 NNP denotes Ransom
T2963 3333-3335 FW denotes et
T2964 3336-3339 FW denotes al.
T2965 3340-3344 CD denotes 1996
T2966 3344-3345 -RRB- denotes )
T2967 3345-3346 . denotes .
T2968 3346-3484 sentence denotes We named one of these zebrafish genes moonshine (mon), and another group named a noncomplementing allele vampire (Weinstein et al. 1996).
T2969 3347-3349 PRP denotes We
T2970 3350-3355 VBD denotes named
T2971 3356-3359 CD denotes one
T2972 3360-3362 IN denotes of
T2973 3363-3368 DT denotes these
T2974 3379-3384 NNS denotes genes
T2975 3369-3378 NN denotes zebrafish
T2976 3385-3394 NN denotes moonshine
T2977 3395-3396 -LRB- denotes (
T2978 3396-3399 NN denotes mon
T2979 3399-3400 -RRB- denotes )
T2980 3400-3402 , denotes ,
T2981 3402-3405 CC denotes and
T2982 3406-3413 DT denotes another
T2983 3414-3419 NN denotes group
T2984 3420-3425 VBD denotes named
T2985 3426-3427 DT denotes a
T2986 3445-3451 NN denotes allele
T2987 3428-3444 JJ denotes noncomplementing
T2988 3452-3459 NN denotes vampire
T2989 3460-3461 -LRB- denotes (
T2990 3461-3470 NNP denotes Weinstein
T2991 3471-3473 FW denotes et
T2992 3474-3477 FW denotes al.
T2993 3478-3482 CD denotes 1996
T2994 3482-3483 -RRB- denotes )
T2995 3483-3484 . denotes .
T2996 3484-3670 sentence denotes Here, we have determined that mutations in the mon gene cause a disruption in both primitive embryonic and definitive adult hematopoiesis, resulting in a severe loss of erythroid cells.
T2997 3485-3489 RB denotes Here
T2998 3499-3509 VBN denotes determined
T2999 3489-3491 , denotes ,
T3000 3491-3493 PRP denotes we
T3001 3494-3498 VBP denotes have
T3002 3510-3514 IN denotes that
T3003 3541-3546 VBP denotes cause
T3004 3515-3524 NNS denotes mutations
T3005 3525-3527 IN denotes in
T3006 3528-3531 DT denotes the
T3007 3536-3540 NN denotes gene
T3008 3532-3535 NN denotes mon
T3009 3547-3548 DT denotes a
T3010 3549-3559 NN denotes disruption
T3011 3560-3562 IN denotes in
T3012 3563-3567 CC denotes both
T3013 3578-3587 JJ denotes embryonic
T3014 3568-3577 JJ denotes primitive
T3015 3609-3622 NN denotes hematopoiesis
T3016 3588-3591 CC denotes and
T3017 3592-3602 JJ denotes definitive
T3018 3603-3608 JJ denotes adult
T3019 3622-3624 , denotes ,
T3020 3624-3633 VBG denotes resulting
T3021 3634-3636 IN denotes in
T3022 3637-3638 DT denotes a
T3023 3646-3650 NN denotes loss
T3024 3639-3645 JJ denotes severe
T3025 3651-3653 IN denotes of
T3026 3654-3663 JJ denotes erythroid
T3027 3664-3669 NNS denotes cells
T3028 3669-3670 . denotes .
T3029 3670-3831 sentence denotes Erythroid progenitor cells in mon mutants are initially present, but fail to express normal levels of hematopoietic transcription factors and undergo apoptosis.
T3030 3671-3680 JJ denotes Erythroid
T3031 3692-3697 NNS denotes cells
T3032 3681-3691 NN denotes progenitor
T3033 3713-3716 VBP denotes are
T3034 3698-3700 IN denotes in
T3035 3701-3704 NN denotes mon
T3036 3705-3712 NNS denotes mutants
T3037 3717-3726 RB denotes initially
T3038 3727-3734 JJ denotes present
T3039 3734-3736 , denotes ,
T3040 3736-3739 CC denotes but
T3041 3740-3744 VBP denotes fail
T3042 3745-3747 TO denotes to
T3043 3748-3755 VB denotes express
T3044 3756-3762 JJ denotes normal
T3045 3763-3769 NNS denotes levels
T3046 3770-3772 IN denotes of
T3047 3773-3786 JJ denotes hematopoietic
T3048 3801-3808 NNS denotes factors
T3049 3787-3800 NN denotes transcription
T3050 3809-3812 CC denotes and
T3051 3813-3820 VB denotes undergo
T3052 3821-3830 NN denotes apoptosis
T3053 3830-3831 . denotes .
T3054 3831-4144 sentence denotes Positional cloning identifies the mon gene as the zebrafish ortholog of mammalian transcriptional intermediary factor 1γ (TIF1γ), a member of the TIF1 family of transcriptional coactivators and corepressors (Le Douarin et al. 1995; Friedman et al. 1996; Kim et al. 1996; Venturini et al. 1999; Peng et al. 2002).
T3055 3832-3842 JJ denotes Positional
T3056 3843-3850 NN denotes cloning
T3057 3851-3861 VBZ denotes identifies
T3058 3862-3865 DT denotes the
T3059 3870-3874 NN denotes gene
T3060 3866-3869 NN denotes mon
T3061 3875-3877 IN denotes as
T3062 3878-3881 DT denotes the
T3063 3892-3900 NN denotes ortholog
T3064 3882-3891 NN denotes zebrafish
T3065 3901-3903 IN denotes of
T3066 3904-3913 JJ denotes mammalian
T3067 3950-3952 NN denotes 1γ
T3068 3914-3929 JJ denotes transcriptional
T3069 3930-3942 JJ denotes intermediary
T3070 3943-3949 NN denotes factor
T3071 3953-3954 -LRB- denotes (
T3072 3954-3959 NN denotes TIF1γ
T3073 3959-3960 -RRB- denotes )
T3074 3960-3962 , denotes ,
T3075 3962-3963 DT denotes a
T3076 3964-3970 NN denotes member
T3077 3971-3973 IN denotes of
T3078 3974-3977 DT denotes the
T3079 3983-3989 NN denotes family
T3080 3978-3982 NN denotes TIF1
T3081 3990-3992 IN denotes of
T3082 3993-4008 JJ denotes transcriptional
T3083 4009-4021 NNS denotes coactivators
T3084 4022-4025 CC denotes and
T3085 4026-4038 NNS denotes corepressors
T3086 4039-4040 -LRB- denotes (
T3087 4040-4042 NNP denotes Le
T3088 4043-4050 NNP denotes Douarin
T3089 4051-4053 FW denotes et
T3090 4054-4057 FW denotes al.
T3091 4058-4062 CD denotes 1995
T3092 4062-4063 : denotes ;
T3093 4064-4072 NNP denotes Friedman
T3094 4073-4075 FW denotes et
T3095 4076-4079 FW denotes al.
T3096 4080-4084 CD denotes 1996
T3097 4084-4085 : denotes ;
T3098 4086-4089 NNP denotes Kim
T3099 4090-4092 FW denotes et
T3100 4093-4096 FW denotes al.
T3101 4097-4101 CD denotes 1996
T3102 4101-4102 : denotes ;
T3103 4103-4112 NNP denotes Venturini
T3104 4113-4115 FW denotes et
T3105 4116-4119 FW denotes al.
T3106 4120-4124 CD denotes 1999
T3107 4124-4125 : denotes ;
T3108 4126-4130 NNP denotes Peng
T3109 4131-4133 FW denotes et
T3110 4134-4137 FW denotes al.
T3111 4138-4142 CD denotes 2002
T3112 4142-4143 -RRB- denotes )
T3113 4143-4144 . denotes .
T3114 4144-4373 sentence denotes The three members of the vertebrate TIF1 family (α, β, and γ) are large nuclear proteins that each contain an N-terminal RBCC or TRIM domain (Reymond et al. 2001) composed of a RING finger, two B-boxes, and a coiled-coil domain.
T3115 4145-4148 DT denotes The
T3116 4155-4162 NNS denotes members
T3117 4149-4154 CD denotes three
T3118 4207-4210 VBP denotes are
T3119 4163-4165 IN denotes of
T3120 4166-4169 DT denotes the
T3121 4186-4192 NN denotes family
T3122 4170-4180 NN denotes vertebrate
T3123 4181-4185 NN denotes TIF1
T3124 4193-4194 -LRB- denotes (
T3125 4194-4195 NN denotes α
T3126 4195-4197 , denotes ,
T3127 4197-4198 NN denotes β
T3128 4198-4200 , denotes ,
T3129 4200-4203 CC denotes and
T3130 4204-4205 SYM denotes γ
T3131 4205-4206 -RRB- denotes )
T3132 4211-4216 JJ denotes large
T3133 4225-4233 NN denotes proteins
T3134 4217-4224 JJ denotes nuclear
T3135 4234-4238 WDT denotes that
T3136 4244-4251 VBP denotes contain
T3137 4239-4243 DT denotes each
T3138 4252-4254 DT denotes an
T3139 4266-4270 NN denotes RBCC
T3140 4255-4256 NN denotes N
T3141 4257-4265 JJ denotes terminal
T3142 4256-4257 HYPH denotes -
T3143 4271-4273 CC denotes or
T3144 4274-4278 NN denotes TRIM
T3145 4279-4285 NN denotes domain
T3146 4286-4287 -LRB- denotes (
T3147 4287-4294 NNP denotes Reymond
T3148 4295-4297 FW denotes et
T3149 4298-4301 FW denotes al.
T3150 4302-4306 CD denotes 2001
T3151 4306-4307 -RRB- denotes )
T3152 4308-4316 VBN denotes composed
T3153 4317-4319 IN denotes of
T3154 4320-4321 DT denotes a
T3155 4327-4333 NN denotes finger
T3156 4322-4326 NN denotes RING
T3157 4333-4335 , denotes ,
T3158 4335-4338 CD denotes two
T3159 4341-4346 NNS denotes boxes
T3160 4339-4340 NN denotes B
T3161 4340-4341 HYPH denotes -
T3162 4346-4348 , denotes ,
T3163 4348-4351 CC denotes and
T3164 4352-4353 DT denotes a
T3165 4366-4372 NN denotes domain
T3166 4354-4360 JJ denotes coiled
T3167 4361-4365 NN denotes coil
T3168 4360-4361 HYPH denotes -
T3169 4372-4373 . denotes .
T3170 4373-4517 sentence denotes TIF1 family members also contain a C-terminal plant homeodomain finger and bromodomain that are characteristic of chromatin remodeling factors.
T3171 4374-4378 NN denotes TIF1
T3172 4386-4393 NNS denotes members
T3173 4379-4385 NN denotes family
T3174 4399-4406 VBP denotes contain
T3175 4394-4398 RB denotes also
T3176 4407-4408 DT denotes a
T3177 4438-4444 NN denotes finger
T3178 4409-4410 NN denotes C
T3179 4410-4411 HYPH denotes -
T3180 4411-4419 JJ denotes terminal
T3181 4420-4425 NN denotes plant
T3182 4426-4437 NN denotes homeodomain
T3183 4445-4448 CC denotes and
T3184 4449-4460 NN denotes bromodomain
T3185 4461-4465 WDT denotes that
T3186 4466-4469 VBP denotes are
T3187 4470-4484 JJ denotes characteristic
T3188 4485-4487 IN denotes of
T3189 4488-4497 NN denotes chromatin
T3190 4498-4508 VBG denotes remodeling
T3191 4509-4516 NNS denotes factors
T3192 4516-4517 . denotes .
T3193 4517-4711 sentence denotes TIF1α has been shown to associate with a variety of ligand-bound nuclear hormone receptors (Le Douarin et al. 1995) and function as a coactivator for retinoic acid receptors (Zhong et al.1999).
T3194 4518-4523 NN denotes TIF1α
T3195 4533-4538 VBN denotes shown
T3196 4524-4527 VBZ denotes has
T3197 4528-4532 VBN denotes been
T3198 4539-4541 TO denotes to
T3199 4542-4551 VB denotes associate
T3200 4552-4556 IN denotes with
T3201 4557-4558 DT denotes a
T3202 4559-4566 NN denotes variety
T3203 4567-4569 IN denotes of
T3204 4570-4576 NN denotes ligand
T3205 4599-4608 NNS denotes receptors
T3206 4576-4577 HYPH denotes -
T3207 4577-4582 VBN denotes bound
T3208 4583-4590 JJ denotes nuclear
T3209 4591-4598 NN denotes hormone
T3210 4609-4610 -LRB- denotes (
T3211 4610-4612 NNP denotes Le
T3212 4613-4620 NNP denotes Douarin
T3213 4621-4623 FW denotes et
T3214 4624-4627 FW denotes al.
T3215 4628-4632 CD denotes 1995
T3216 4632-4633 -RRB- denotes )
T3217 4634-4637 CC denotes and
T3218 4638-4646 VB denotes function
T3219 4647-4649 IN denotes as
T3220 4650-4651 DT denotes a
T3221 4652-4663 NN denotes coactivator
T3222 4664-4667 IN denotes for
T3223 4668-4676 JJ denotes retinoic
T3224 4677-4681 NN denotes acid
T3225 4682-4691 NNS denotes receptors
T3226 4692-4693 -LRB- denotes (
T3227 4693-4698 NNP denotes Zhong
T3228 4699-4701 FW denotes et
T3230 4705-4709 CD denotes 1999
T3231 4709-4710 -RRB- denotes )
T3232 4710-4711 . denotes .
T3233 4711-4895 sentence denotes TIF1β has been shown to act as a corepressor for the large family of Krüppel-associated box (KRAB) domain zinc-finger transcription factors (Friedman et al. 1996; Abrink et al. 2001).
T3234 4712-4717 NN denotes TIF1β
T3235 4727-4732 VBN denotes shown
T3236 4718-4721 VBZ denotes has
T3237 4722-4726 VBN denotes been
T3238 4733-4735 TO denotes to
T3239 4736-4739 VB denotes act
T3240 4740-4742 IN denotes as
T3241 4743-4744 DT denotes a
T3242 4745-4756 NN denotes corepressor
T3243 4757-4760 IN denotes for
T3244 4761-4764 DT denotes the
T3245 4771-4777 NN denotes family
T3246 4765-4770 JJ denotes large
T3247 4778-4780 IN denotes of
T3248 4781-4788 NN denotes Krüppel
T3249 4789-4799 VBN denotes associated
T3250 4788-4789 HYPH denotes -
T3251 4800-4803 NN denotes box
T3252 4811-4817 NN denotes domain
T3253 4804-4805 -LRB- denotes (
T3254 4805-4809 NN denotes KRAB
T3255 4809-4810 -RRB- denotes )
T3256 4844-4851 NNS denotes factors
T3257 4818-4822 NN denotes zinc
T3258 4823-4829 NN denotes finger
T3259 4822-4823 HYPH denotes -
T3260 4830-4843 NN denotes transcription
T3261 4852-4853 -LRB- denotes (
T3262 4853-4861 NNP denotes Friedman
T3263 4862-4864 FW denotes et
T3264 4865-4868 FW denotes al.
T3265 4869-4873 CD denotes 1996
T3266 4873-4874 : denotes ;
T3267 4875-4881 NNP denotes Abrink
T3268 4882-4884 FW denotes et
T3269 4885-4888 FW denotes al.
T3270 4889-4893 CD denotes 2001
T3271 4893-4894 -RRB- denotes )
T3272 4894-4895 . denotes .
T3273 4895-5076 sentence denotes In contrast, TIF1γ does not associate directly with either nuclear receptors or KRAB domains that bind to the other TIF1 family members (Venturini et al. 1999; Abrink et al. 2001).
T3274 4896-4898 IN denotes In
T3275 4924-4933 VB denotes associate
T3276 4899-4907 NN denotes contrast
T3277 4907-4909 , denotes ,
T3278 4909-4914 NN denotes TIF1γ
T3279 4915-4919 VBZ denotes does
T3280 4920-4923 RB denotes not
T3281 4934-4942 RB denotes directly
T3282 4943-4947 IN denotes with
T3283 4948-4954 CC denotes either
T3284 4963-4972 NNS denotes receptors
T3285 4955-4962 JJ denotes nuclear
T3286 4973-4975 CC denotes or
T3287 4976-4980 NN denotes KRAB
T3288 4981-4988 NNS denotes domains
T3289 4989-4993 WDT denotes that
T3290 4994-4998 VBP denotes bind
T3291 4999-5001 IN denotes to
T3292 5002-5005 DT denotes the
T3293 5024-5031 NNS denotes members
T3294 5006-5011 JJ denotes other
T3295 5012-5016 NN denotes TIF1
T3296 5017-5023 NN denotes family
T3297 5032-5033 -LRB- denotes (
T3298 5033-5042 NNP denotes Venturini
T3299 5043-5045 FW denotes et
T3300 5046-5049 FW denotes al.
T3301 5050-5054 CD denotes 1999
T3302 5054-5055 : denotes ;
T3303 5056-5062 NNP denotes Abrink
T3304 5063-5065 FW denotes et
T3305 5066-5069 FW denotes al.
T3306 5070-5074 CD denotes 2001
T3307 5074-5075 -RRB- denotes )
T3308 5075-5076 . denotes .
T3309 5076-5221 sentence denotes Biochemical studies also demonstrate that TIF1γ forms both homo-oligomers and hetero-oligomers with TIF1α but not with TIF1β (Peng et al. 2002).
T3310 5077-5088 JJ denotes Biochemical
T3311 5089-5096 NNS denotes studies
T3312 5102-5113 VBP denotes demonstrate
T3313 5097-5101 RB denotes also
T3314 5114-5118 IN denotes that
T3315 5125-5130 VBZ denotes forms
T3316 5119-5124 NN denotes TIF1γ
T3317 5131-5135 CC denotes both
T3318 5136-5150 NNS denotes homo-oligomers
T3319 5151-5154 CC denotes and
T3320 5155-5171 NNS denotes hetero-oligomers
T3321 5172-5176 IN denotes with
T3322 5177-5182 NN denotes TIF1α
T3323 5183-5186 CC denotes but
T3324 5187-5190 RB denotes not
T3325 5191-5195 IN denotes with
T3326 5196-5201 NN denotes TIF1β
T3327 5202-5203 -LRB- denotes (
T3328 5203-5207 NNP denotes Peng
T3329 5208-5210 FW denotes et
T3330 5211-5214 FW denotes al.
T3331 5215-5219 CD denotes 2002
T3332 5219-5220 -RRB- denotes )
T3333 5220-5221 . denotes .
T3334 5221-5477 sentence denotes The murine Tif1α and Tif1γ genes have not yet been subjected to gene targeting experiments, whereas analysis of mouse mutants demonstrates that Tif1β is required for postimplantation embryogenesis and mesoderm induction in particular (Cammas et al. 2000).
T3335 5222-5225 DT denotes The
T3336 5249-5254 NNS denotes genes
T3337 5226-5232 JJ denotes murine
T3338 5233-5238 NN denotes Tif1α
T3339 5239-5242 CC denotes and
T3340 5243-5248 NN denotes Tif1γ
T3341 5273-5282 VBN denotes subjected
T3342 5255-5259 VBP denotes have
T3343 5260-5263 RB denotes not
T3344 5264-5267 RB denotes yet
T3345 5268-5272 VBN denotes been
T3346 5283-5285 IN denotes to
T3347 5286-5290 NN denotes gene
T3348 5291-5300 VBG denotes targeting
T3349 5301-5312 NNS denotes experiments
T3350 5312-5314 , denotes ,
T3351 5314-5321 IN denotes whereas
T3352 5348-5360 VBZ denotes demonstrates
T3353 5322-5330 NN denotes analysis
T3354 5331-5333 IN denotes of
T3355 5334-5339 NN denotes mouse
T3356 5340-5347 NNS denotes mutants
T3357 5361-5365 IN denotes that
T3358 5375-5383 VBN denotes required
T3359 5366-5371 NN denotes Tif1β
T3360 5372-5374 VBZ denotes is
T3361 5384-5387 IN denotes for
T3362 5388-5404 NN denotes postimplantation
T3363 5405-5418 NN denotes embryogenesis
T3364 5419-5422 CC denotes and
T3365 5423-5431 NN denotes mesoderm
T3366 5432-5441 NN denotes induction
T3367 5442-5444 IN denotes in
T3368 5445-5455 JJ denotes particular
T3369 5456-5457 -LRB- denotes (
T3370 5457-5463 NNP denotes Cammas
T3371 5464-5466 FW denotes et
T3372 5467-5470 FW denotes al.
T3373 5471-5475 CD denotes 2000
T3374 5475-5476 -RRB- denotes )
T3375 5476-5477 . denotes .
T3376 5477-5652 sentence denotes Taken together, these studies suggest that a major function of TIF1 family members is to link DNA-binding proteins with other coactivators or corepressors during development.
T3377 5478-5483 VBN denotes Taken
T3378 5508-5515 VBP denotes suggest
T3379 5484-5492 RB denotes together
T3380 5492-5494 , denotes ,
T3381 5494-5499 DT denotes these
T3382 5500-5507 NNS denotes studies
T3383 5516-5520 IN denotes that
T3384 5561-5563 VBZ denotes is
T3385 5521-5522 DT denotes a
T3386 5529-5537 NN denotes function
T3387 5523-5528 JJ denotes major
T3388 5538-5540 IN denotes of
T3389 5541-5545 NN denotes TIF1
T3390 5553-5560 NNS denotes members
T3391 5546-5552 NN denotes family
T3392 5564-5566 TO denotes to
T3393 5567-5571 VB denotes link
T3394 5572-5575 NN denotes DNA
T3395 5584-5592 NN denotes proteins
T3396 5575-5576 HYPH denotes -
T3397 5576-5583 VBG denotes binding
T3398 5593-5597 IN denotes with
T3399 5598-5603 JJ denotes other
T3400 5604-5616 NNS denotes coactivators
T3401 5617-5619 CC denotes or
T3402 5620-5632 NNS denotes corepressors
T3403 5633-5639 IN denotes during
T3404 5640-5651 NN denotes development
T3405 5651-5652 . denotes .
T3406 5652-5774 sentence denotes Our studies establish that tif1γ functions as an essential regulator of embryonic and adult hematopoiesis in vertebrates.
T3407 5653-5656 PRP$ denotes Our
T3408 5657-5664 NNS denotes studies
T3409 5665-5674 VBP denotes establish
T3410 5675-5679 IN denotes that
T3411 5686-5695 VBZ denotes functions
T3412 5680-5685 NN denotes tif1γ
T3413 5696-5698 IN denotes as
T3414 5699-5701 DT denotes an
T3415 5712-5721 NN denotes regulator
T3416 5702-5711 JJ denotes essential
T3417 5722-5724 IN denotes of
T3418 5725-5734 JJ denotes embryonic
T3419 5745-5758 NN denotes hematopoiesis
T3420 5735-5738 CC denotes and
T3421 5739-5744 JJ denotes adult
T3422 5759-5761 IN denotes in
T3423 5762-5773 NNS denotes vertebrates
T3424 5773-5774 . denotes .
T3425 5774-5891 sentence denotes Cell transplantation studies demonstrate that tif1γ acts in a cell-autonomous manner during embryonic hematopoiesis.
T3426 5775-5779 NN denotes Cell
T3427 5796-5803 NNS denotes studies
T3428 5780-5795 NN denotes transplantation
T3429 5804-5815 VBP denotes demonstrate
T3430 5816-5820 IN denotes that
T3431 5827-5831 VBZ denotes acts
T3432 5821-5826 NN denotes tif1γ
T3433 5832-5834 IN denotes in
T3434 5835-5836 DT denotes a
T3435 5853-5859 NN denotes manner
T3436 5837-5841 NN denotes cell
T3437 5841-5842 HYPH denotes -
T3438 5842-5852 JJ denotes autonomous
T3439 5860-5866 IN denotes during
T3440 5867-5876 JJ denotes embryonic
T3441 5877-5890 NN denotes hematopoiesis
T3442 5890-5891 . denotes .
T3443 5891-6034 sentence denotes The tif1γ gene is expressed specifically in ventral mesoderm and hematopoietic progenitors, then downregulated as erythroid maturation occurs.
T3444 5892-5895 DT denotes The
T3445 5902-5906 NN denotes gene
T3446 5896-5901 NN denotes tif1γ
T3447 5910-5919 VBN denotes expressed
T3448 5907-5909 VBZ denotes is
T3449 5920-5932 RB denotes specifically
T3450 5933-5935 IN denotes in
T3451 5936-5943 JJ denotes ventral
T3452 5944-5952 NN denotes mesoderm
T3453 5971-5982 NNS denotes progenitors
T3454 5953-5956 CC denotes and
T3455 5957-5970 JJ denotes hematopoietic
T3456 5982-5984 , denotes ,
T3457 5984-5988 RB denotes then
T3458 5989-6002 VBN denotes downregulated
T3459 6003-6005 IN denotes as
T3460 6027-6033 VBZ denotes occurs
T3461 6006-6015 JJ denotes erythroid
T3462 6016-6026 NN denotes maturation
T3463 6033-6034 . denotes .
T3464 6034-6166 sentence denotes Tif1γ protein localizes to a novel class of nuclear bodies in both primary mouse embryo fibroblasts and erythroleukemia cell lines.
T3465 6035-6040 NN denotes Tif1γ
T3466 6041-6048 NN denotes protein
T3467 6049-6058 VBZ denotes localizes
T3468 6059-6061 IN denotes to
T3469 6062-6063 DT denotes a
T3470 6070-6075 NN denotes class
T3471 6064-6069 JJ denotes novel
T3472 6076-6078 IN denotes of
T3473 6079-6086 JJ denotes nuclear
T3474 6087-6093 NNS denotes bodies
T3475 6094-6096 IN denotes in
T3476 6097-6101 CC denotes both
T3477 6123-6134 NNS denotes fibroblasts
T3478 6102-6109 JJ denotes primary
T3479 6110-6115 NN denotes mouse
T3480 6116-6122 NN denotes embryo
T3481 6135-6138 CC denotes and
T3482 6139-6154 NN denotes erythroleukemia
T3483 6160-6165 NNS denotes lines
T3484 6155-6159 NN denotes cell
T3485 6165-6166 . denotes .
T3486 6166-6281 sentence denotes Taken together, our studies demonstrate that Tif1γ is required for normal erythroid cell development and survival.
T3487 6167-6172 VBN denotes Taken
T3488 6195-6206 VBP denotes demonstrate
T3489 6173-6181 RB denotes together
T3490 6181-6183 , denotes ,
T3491 6183-6186 PRP$ denotes our
T3492 6187-6194 NNS denotes studies
T3493 6207-6211 IN denotes that
T3494 6221-6229 VBN denotes required
T3495 6212-6217 NN denotes Tif1γ
T3496 6218-6220 VBZ denotes is
T3497 6230-6233 IN denotes for
T3498 6234-6240 JJ denotes normal
T3499 6256-6267 NN denotes development
T3500 6241-6250 JJ denotes erythroid
T3501 6251-6255 NN denotes cell
T3502 6268-6271 CC denotes and
T3503 6272-6280 NN denotes survival
T3504 6280-6281 . denotes .
T4640 0-3 DT denotes The
T4641 6310-6314 NN denotes Gene
T4642 4-13 NN denotes Zebrafish
T4643 14-6309 NN denotes moonshine Gene Encodes Transcriptional Intermediary Factor 1γ, an Essential Regulator of Hematopoiesis moonshine/tif1γ in Hematopoiesis Abstract Hematopoiesis is precisely orchestrated by lineage-specific DNA-binding proteins that regulate transcription in concert with coactivators and corepressors. Mutations in the zebrafish moonshine (mon) gene specifically disrupt both embryonic and adult hematopoiesis, resulting in severe red blood cell aplasia. We report that mon encodes the zebrafish ortholog of mammalian transcriptional intermediary factor 1γ (TIF1γ) (or TRIM33), a member of the TIF1 family of coactivators and corepressors. During development, hematopoietic progenitor cells in mon mutants fail to express normal levels of hematopoietic transcription factors, including gata1, and undergo apoptosis. Three different mon mutant alleles each encode premature stop codons, and enforced expression of wild-type tif1γ mRNA rescues embryonic hematopoiesis in homozygous mon mutants. Surprisingly, a high level of zygotic tif1γ mRNA expression delineates ventral mesoderm during hematopoietic stem cell and progenitor formation prior to gata1 expression. Transplantation studies reveal that tif1γ functions in a cell-autonomous manner during the differentiation of erythroid precursors. Studies in murine erythroid cell lines demonstrate that Tif1γ protein is localized within novel nuclear foci, and expression decreases during erythroid cell maturation. Our results establish a major role for this transcriptional intermediary factor in the differentiation of hematopoietic cells in vertebrates. A new gene acting early in red blood cell development is discovered by genetic analysis in zebrafish. The gene encodes a member of a well-known family of transcription factors Introduction Hematopoiesis involves the coordinated processes of cell proliferation and differentiation of a relatively small number of progenitor cells into billions of circulating red and white blood cells (Thisse and Zon 2002). Hematopoiesis in vertebrates, from zebrafish to humans, is an evolutionarily conserved program that produces two waves of stem or progenitor cells that differ both in their embryonic origins and in the lineages of differentiated blood cells produced (Palis and Yoder 2001; Orkin and Zon 2002; Galloway and Zon 2003). The first, or primitive, wave of hematopoiesis originates from ventral mesoderm and gives rise to progenitor cells that differentiate in embryonic blood islands. The primitive wave of hematopoiesis produces a burst of embryonic erythrocytes and macrophages. The second, or definitive, wave of hematopoiesis arises from self-renewing stem cells that develop primarily in the intraembryonic aorta–gonad–mesonephros region. These definitive hematopoietic stem cells seed the later developing marrow spaces, to produce all lineages of adult blood cells, including definitive erythrocytes, myeloid cells, and lymphocytes. We have undertaken a genetic approach to characterize genes that control hematopoiesis using the zebrafish as a model system (Thisse and Zon 2002). As part of a large-scale forward genetic screen, we previously identified bloodless zebrafish mutants that failed to express the erythroid transcription factor gata1 normally in embryonic hematopoietic precursors (Ransom et al. 1996). We named one of these zebrafish genes moonshine (mon), and another group named a noncomplementing allele vampire (Weinstein et al. 1996). Here, we have determined that mutations in the mon gene cause a disruption in both primitive embryonic and definitive adult hematopoiesis, resulting in a severe loss of erythroid cells. Erythroid progenitor cells in mon mutants are initially present, but fail to express normal levels of hematopoietic transcription factors and undergo apoptosis. Positional cloning identifies the mon gene as the zebrafish ortholog of mammalian transcriptional intermediary factor 1γ (TIF1γ), a member of the TIF1 family of transcriptional coactivators and corepressors (Le Douarin et al. 1995; Friedman et al. 1996; Kim et al. 1996; Venturini et al. 1999; Peng et al. 2002). The three members of the vertebrate TIF1 family (α, β, and γ) are large nuclear proteins that each contain an N-terminal RBCC or TRIM domain (Reymond et al. 2001) composed of a RING finger, two B-boxes, and a coiled-coil domain. TIF1 family members also contain a C-terminal plant homeodomain finger and bromodomain that are characteristic of chromatin remodeling factors. TIF1α has been shown to associate with a variety of ligand-bound nuclear hormone receptors (Le Douarin et al. 1995) and function as a coactivator for retinoic acid receptors (Zhong et al.1999). TIF1β has been shown to act as a corepressor for the large family of Krüppel-associated box (KRAB) domain zinc-finger transcription factors (Friedman et al. 1996; Abrink et al. 2001). In contrast, TIF1γ does not associate directly with either nuclear receptors or KRAB domains that bind to the other TIF1 family members (Venturini et al. 1999; Abrink et al. 2001). Biochemical studies also demonstrate that TIF1γ forms both homo-oligomers and hetero-oligomers with TIF1α but not with TIF1β (Peng et al. 2002). The murine Tif1α and Tif1γ genes have not yet been subjected to gene targeting experiments, whereas analysis of mouse mutants demonstrates that Tif1β is required for postimplantation embryogenesis and mesoderm induction in particular (Cammas et al. 2000). Taken together, these studies suggest that a major function of TIF1 family members is to link DNA-binding proteins with other coactivators or corepressors during development. Our studies establish that tif1γ functions as an essential regulator of embryonic and adult hematopoiesis in vertebrates. Cell transplantation studies demonstrate that tif1γ acts in a cell-autonomous manner during embryonic hematopoiesis. The tif1γ gene is expressed specifically in ventral mesoderm and hematopoietic progenitors, then downregulated as erythroid maturation occurs. Tif1γ protein localizes to a novel class of nuclear bodies in both primary mouse embryo fibroblasts and erythroleukemia cell lines. Taken together, our studies demonstrate that Tif1γ is required for normal erythroid cell development and survival. Results The Zebrafish mon
T4644 6315-6317 VBZ denotes Is
T4645 6318-6327 JJ denotes Essential
T4646 6328-6331 IN denotes for
T4647 6332-6336 CC denotes Both
T4648 6337-6346 JJ denotes Primitive
T4649 6362-6376 NN denotes Erythropoiesis
T4650 6347-6350 CC denotes and
T4651 6351-6361 JJ denotes Definitive
T4652 6376-6550 sentence denotes In order to determine when the mon gene is required in development, we first examined hematopoietic gene expression and apoptosis in zebrafish homozygous mon mutant embryos.
T4653 6377-6379 IN denotes In
T4654 6454-6462 VBD denotes examined
T4655 6380-6385 NN denotes order
T4656 6386-6388 TO denotes to
T4657 6389-6398 VB denotes determine
T4658 6399-6403 WRB denotes when
T4659 6420-6428 VBN denotes required
T4660 6404-6407 DT denotes the
T4661 6412-6416 NN denotes gene
T4662 6408-6411 NN denotes mon
T4663 6417-6419 VBZ denotes is
T4664 6429-6431 IN denotes in
T4665 6432-6443 NN denotes development
T4666 6443-6445 , denotes ,
T4667 6445-6447 PRP denotes we
T4668 6448-6453 RB denotes first
T4669 6463-6476 JJ denotes hematopoietic
T4670 6482-6492 NN denotes expression
T4671 6477-6481 NN denotes gene
T4672 6493-6496 CC denotes and
T4673 6497-6506 NN denotes apoptosis
T4674 6507-6509 IN denotes in
T4675 6510-6519 NN denotes zebrafish
T4676 6542-6549 NNS denotes embryos
T4677 6520-6530 JJ denotes homozygous
T4678 6531-6534 NN denotes mon
T4679 6535-6541 JJ denotes mutant
T4680 6549-6550 . denotes .
T4681 6550-6704 sentence denotes During embryogenesis, homozygous zebrafish mon mutants have no red blood cells (RBCs) visible in circulation (Ransom et al. 1996; Weinstein et al. 1996).
T4682 6551-6557 IN denotes During
T4683 6606-6610 VBP denotes have
T4684 6558-6571 NN denotes embryogenesis
T4685 6571-6573 , denotes ,
T4686 6573-6583 JJ denotes homozygous
T4687 6598-6605 NNS denotes mutants
T4688 6584-6593 NN denotes zebrafish
T4689 6594-6597 NN denotes mon
T4690 6611-6613 DT denotes no
T4691 6624-6629 NNS denotes cells
T4692 6614-6617 JJ denotes red
T4693 6618-6623 NN denotes blood
T4694 6630-6631 -LRB- denotes (
T4695 6631-6635 NNS denotes RBCs
T4696 6635-6636 -RRB- denotes )
T4697 6637-6644 JJ denotes visible
T4698 6645-6647 IN denotes in
T4699 6648-6659 NN denotes circulation
T4700 6660-6661 -LRB- denotes (
T4701 6661-6667 NNP denotes Ransom
T4702 6668-6670 FW denotes et
T4703 6671-6674 FW denotes al.
T4704 6675-6679 CD denotes 1996
T4705 6679-6680 : denotes ;
T4706 6681-6690 NNP denotes Weinstein
T4707 6691-6693 FW denotes et
T4708 6694-6697 FW denotes al.
T4709 6698-6702 CD denotes 1996
T4710 6702-6703 -RRB- denotes )
T4711 6703-6704 . denotes .
T4712 6704-7034 sentence denotes The mon mutants initiate expression of gata1 in hematopoietic cells around the five-somite stage, similar to wild-type embryos (data not shown); however, based on TUNEL staining, the differentiating erythroid cells undergo programmed cell death from the 12-somite stage to 22 h postfertilization (hpf) (Figure 1A and 1B, arrows).
T4713 6705-6708 DT denotes The
T4714 6713-6720 NNS denotes mutants
T4715 6709-6712 NN denotes mon
T4716 6721-6729 VBP denotes initiate
T4717 6920-6927 VBP denotes undergo
T4718 6730-6740 NN denotes expression
T4719 6741-6743 IN denotes of
T4720 6744-6749 NN denotes gata1
T4721 6750-6752 IN denotes in
T4722 6753-6766 JJ denotes hematopoietic
T4723 6767-6772 NNS denotes cells
T4724 6773-6779 IN denotes around
T4725 6780-6783 DT denotes the
T4726 6796-6801 NN denotes stage
T4727 6784-6788 CD denotes five
T4728 6789-6795 NN denotes somite
T4729 6788-6789 HYPH denotes -
T4730 6801-6803 , denotes ,
T4731 6803-6810 JJ denotes similar
T4732 6811-6813 IN denotes to
T4733 6814-6818 JJ denotes wild
T4734 6819-6823 NN denotes type
T4735 6818-6819 HYPH denotes -
T4736 6824-6831 NNS denotes embryos
T4737 6832-6833 -LRB- denotes (
T4738 6842-6847 VBN denotes shown
T4739 6833-6837 NNS denotes data
T4740 6838-6841 RB denotes not
T4741 6847-6848 -RRB- denotes )
T4742 6848-6849 , denotes ;
T4743 6850-6857 RB denotes however
T4744 6857-6859 , denotes ,
T4745 6859-6864 VBN denotes based
T4746 6865-6867 IN denotes on
T4747 6868-6873 NN denotes TUNEL
T4748 6874-6882 NN denotes staining
T4749 6882-6884 , denotes ,
T4750 6884-6887 DT denotes the
T4751 6914-6919 NNS denotes cells
T4752 6888-6903 VBG denotes differentiating
T4753 6904-6913 NN denotes erythroid
T4754 6928-6938 JJ denotes programmed
T4755 6944-6949 NN denotes death
T4756 6939-6943 NN denotes cell
T4757 6950-6954 IN denotes from
T4758 6955-6958 DT denotes the
T4759 6969-6974 NN denotes stage
T4760 6959-6961 CD denotes 12
T4761 6962-6968 NN denotes somite
T4762 6961-6962 HYPH denotes -
T4763 6975-6977 IN denotes to
T4764 6978-6980 CD denotes 22
T4765 6981-6982 NN denotes h
T4766 6983-7000 NN denotes postfertilization
T4767 7001-7002 -LRB- denotes (
T4768 7002-7005 NN denotes hpf
T4769 7005-7006 -RRB- denotes )
T4770 7007-7008 -LRB- denotes (
T4771 7026-7032 NNS denotes arrows
T4772 7008-7014 NN denotes Figure
T4773 7015-7017 CD denotes 1A
T4774 7018-7021 CC denotes and
T4775 7022-7024 CD denotes 1B
T4776 7024-7026 , denotes ,
T4777 7032-7033 -RRB- denotes )
T4778 7033-7034 . denotes .
T4779 7034-7092 sentence denotes At 12 somites, gata1 expression is only slightly reduced.
T4780 7035-7037 IN denotes At
T4781 7067-7069 VBZ denotes is
T4782 7038-7040 CD denotes 12
T4783 7041-7048 NNS denotes somites
T4784 7048-7050 , denotes ,
T4785 7050-7055 NN denotes gata1
T4786 7056-7066 NN denotes expression
T4787 7070-7074 RB denotes only
T4788 7075-7083 RB denotes slightly
T4789 7084-7091 JJ denotes reduced
T4790 7091-7092 . denotes .
T4791 7092-7260 sentence denotes By 18–22 hpf, hematopoietic-specific markers such as gata1, scl, gata2, and ikaros are not detected in the embryonic blood island (Figure 1A and 1B; unpublished data).
T4792 7093-7095 IN denotes By
T4793 7184-7192 VBN denotes detected
T4794 7096-7098 CD denotes 18
T4795 7099-7101 CD denotes 22
T4796 7098-7099 SYM denotes –
T4797 7102-7105 NN denotes hpf
T4798 7105-7107 , denotes ,
T4799 7107-7120 JJ denotes hematopoietic
T4800 7121-7129 JJ denotes specific
T4801 7120-7121 HYPH denotes -
T4802 7130-7137 NNS denotes markers
T4803 7138-7142 JJ denotes such
T4804 7143-7145 IN denotes as
T4805 7146-7151 NN denotes gata1
T4806 7151-7153 , denotes ,
T4807 7153-7156 NN denotes scl
T4808 7156-7158 , denotes ,
T4809 7158-7163 NN denotes gata2
T4810 7163-7165 , denotes ,
T4811 7165-7168 CC denotes and
T4812 7169-7175 NN denotes ikaros
T4813 7176-7179 VBP denotes are
T4814 7180-7183 RB denotes not
T4815 7193-7195 IN denotes in
T4816 7196-7199 DT denotes the
T4817 7216-7222 NN denotes island
T4818 7200-7209 JJ denotes embryonic
T4819 7210-7215 NN denotes blood
T4820 7223-7224 -LRB- denotes (
T4821 7254-7258 NNS denotes data
T4822 7224-7230 NN denotes Figure
T4823 7231-7233 CD denotes 1A
T4824 7234-7237 CC denotes and
T4825 7238-7240 CD denotes 1B
T4826 7240-7241 : denotes ;
T4827 7242-7253 JJ denotes unpublished
T4828 7258-7259 -RRB- denotes )
T4829 7259-7260 . denotes .
T4830 7260-7406 sentence denotes The hematopoietic cells are thus correctly specified early during the development of mon mutant embryos, but these precursors undergo cell death.
T4831 7261-7264 DT denotes The
T4832 7279-7284 NNS denotes cells
T4833 7265-7278 JJ denotes hematopoietic
T4834 7304-7313 VBN denotes specified
T4835 7285-7288 VBP denotes are
T4836 7289-7293 RB denotes thus
T4837 7294-7303 RB denotes correctly
T4838 7314-7319 RB denotes early
T4839 7320-7326 IN denotes during
T4840 7327-7330 DT denotes the
T4841 7331-7342 NN denotes development
T4842 7343-7345 IN denotes of
T4843 7346-7349 NN denotes mon
T4844 7357-7364 NNS denotes embryos
T4845 7350-7356 JJ denotes mutant
T4846 7364-7366 , denotes ,
T4847 7366-7369 CC denotes but
T4848 7370-7375 DT denotes these
T4849 7376-7386 NNS denotes precursors
T4850 7387-7394 VBP denotes undergo
T4851 7395-7399 NN denotes cell
T4852 7400-7405 NN denotes death
T4853 7405-7406 . denotes .
T4854 7406-7537 sentence denotes Based on expression of c-myb and rag1 (Figure 1B, arrows), mon mutants have normal myeloid and lymphoid development, respectively.
T4855 7407-7412 VBN denotes Based
T4856 7478-7482 VBP denotes have
T4857 7413-7415 IN denotes on
T4858 7416-7426 NN denotes expression
T4859 7427-7429 IN denotes of
T4860 7430-7431 NN denotes c
T4861 7432-7435 NN denotes myb
T4862 7431-7432 HYPH denotes -
T4863 7436-7439 CC denotes and
T4864 7440-7444 NN denotes rag1
T4865 7445-7446 -LRB- denotes (
T4866 7457-7463 NNS denotes arrows
T4867 7446-7452 NN denotes Figure
T4868 7453-7455 CD denotes 1B
T4869 7455-7457 , denotes ,
T4870 7463-7464 -RRB- denotes )
T4871 7464-7466 , denotes ,
T4872 7466-7469 NN denotes mon
T4873 7470-7477 NNS denotes mutants
T4874 7483-7489 JJ denotes normal
T4875 7511-7522 NN denotes development
T4876 7490-7497 JJ denotes myeloid
T4877 7498-7501 CC denotes and
T4878 7502-7510 JJ denotes lymphoid
T4879 7522-7524 , denotes ,
T4880 7524-7536 RB denotes respectively
T4881 7536-7537 . denotes .
T4882 7537-7668 sentence denotes In addition to the deficit of RBCs in mon mutants, there is a prominent loss of fin-fold and tail mesenchyme (Ransom et al. 1996).
T4883 7538-7540 IN denotes In
T4884 7595-7597 VBZ denotes is
T4885 7541-7549 NN denotes addition
T4886 7550-7552 IN denotes to
T4887 7553-7556 DT denotes the
T4888 7557-7564 NN denotes deficit
T4889 7565-7567 IN denotes of
T4890 7568-7572 NNS denotes RBCs
T4891 7573-7575 IN denotes in
T4892 7576-7579 NN denotes mon
T4893 7580-7587 NNS denotes mutants
T4894 7587-7589 , denotes ,
T4895 7589-7594 EX denotes there
T4896 7598-7599 DT denotes a
T4897 7610-7614 NN denotes loss
T4898 7600-7609 JJ denotes prominent
T4899 7615-7617 IN denotes of
T4900 7618-7621 NN denotes fin
T4901 7622-7626 NN denotes fold
T4902 7621-7622 HYPH denotes -
T4903 7636-7646 NN denotes mesenchyme
T4904 7627-7630 CC denotes and
T4905 7631-7635 NN denotes tail
T4906 7647-7648 -LRB- denotes (
T4907 7648-7654 NNP denotes Ransom
T4908 7655-7657 FW denotes et
T4909 7658-7661 FW denotes al.
T4910 7662-7666 CD denotes 1996
T4911 7666-7667 -RRB- denotes )
T4912 7667-7668 . denotes .
T4913 7668-7814 sentence denotes TUNEL staining of mon mutants demonstrates extensive apoptosis of mesenchymal cells in the trunk and tail bud regions (Figure 1A and 1B, arrows).
T4914 7669-7674 NN denotes TUNEL
T4915 7675-7683 NN denotes staining
T4916 7699-7711 VBZ denotes demonstrates
T4917 7684-7686 IN denotes of
T4918 7687-7690 NN denotes mon
T4919 7691-7698 NNS denotes mutants
T4920 7712-7721 JJ denotes extensive
T4921 7722-7731 NN denotes apoptosis
T4922 7732-7734 IN denotes of
T4923 7735-7746 JJ denotes mesenchymal
T4924 7747-7752 NNS denotes cells
T4925 7753-7755 IN denotes in
T4926 7756-7759 DT denotes the
T4927 7779-7786 NNS denotes regions
T4928 7760-7765 NN denotes trunk
T4929 7766-7769 CC denotes and
T4930 7770-7774 NN denotes tail
T4931 7775-7778 NN denotes bud
T4932 7787-7788 -LRB- denotes (
T4933 7806-7812 NNS denotes arrows
T4934 7788-7794 NN denotes Figure
T4935 7795-7797 CD denotes 1A
T4936 7798-7801 CC denotes and
T4937 7802-7804 CD denotes 1B
T4938 7804-7806 , denotes ,
T4939 7812-7813 -RRB- denotes )
T4940 7813-7814 . denotes .
T4941 7814-7958 sentence denotes The mon gene is thus required for normal development and survival of both committed erythroid progenitor cells and posterior mesenchymal cells.
T4942 7815-7818 DT denotes The
T4943 7823-7827 NN denotes gene
T4944 7819-7822 NN denotes mon
T4945 7836-7844 VBN denotes required
T4946 7828-7830 VBZ denotes is
T4947 7831-7835 RB denotes thus
T4948 7845-7848 IN denotes for
T4949 7849-7855 JJ denotes normal
T4950 7856-7867 NN denotes development
T4951 7868-7871 CC denotes and
T4952 7872-7880 NN denotes survival
T4953 7881-7883 IN denotes of
T4954 7884-7888 CC denotes both
T4955 7920-7925 NNS denotes cells
T4956 7889-7898 VBN denotes committed
T4957 7899-7908 JJ denotes erythroid
T4958 7909-7919 NN denotes progenitor
T4959 7926-7929 CC denotes and
T4960 7930-7939 JJ denotes posterior
T4961 7952-7957 NNS denotes cells
T4962 7940-7951 JJ denotes mesenchymal
T4963 7957-7958 . denotes .
T4964 7958-8993 sentence denotes Figure 1 Zebrafish mon Mutants Have Severe Defects in Primitive Hematopoiesis (A) Whole-mount TUNEL assays reveal that ventral-posterior mesodermal cells undergo apoptosis in homozygous montg234 mutant embryos. Whole-mount in situ hybridization of gata1 detected at the 12- and 18-somite stage in genotyped embryos. Posterior views, anterior to the left. (B) Extensive apoptosis is visible in the trunk and tail (arrowhead) and also in hematopoietic cells of the embryonic blood island at 22 h of development (arrow). Whole-mount in situ hybridization at 22 hpf including scl, gata2, gata1, ikaros, and myb in montg234 mutants. Expression of myb is greatly reduced in the blood islands because of a loss of erythroid cells, but embryonic macrophages are still present (arrows). The expression of rag1 in thymic T-cells appears normal in mon mutants at 5 d postfertilization (arrow heads). Lateral views of 22 hpf and 5-d-old embryos. We next examined definitive hematopoiesis in rare surviving homozygous adult zebrafish mon mutants.
T4965 8894-8896 PRP denotes We
T4966 8902-8910 VBD denotes examined
T4967 8897-8901 RB denotes next
T4968 8911-8921 JJ denotes definitive
T4969 8922-8935 NN denotes hematopoiesis
T4970 8936-8938 IN denotes in
T4971 8939-8943 JJ denotes rare
T4972 8985-8992 NNS denotes mutants
T4973 8944-8953 VBG denotes surviving
T4974 8954-8964 JJ denotes homozygous
T4975 8981-8984 NN denotes mon
T4976 8965-8970 JJ denotes adult
T4977 8971-8980 NN denotes zebrafish
T4978 8992-8993 . denotes .
T4979 8993-9206 sentence denotes Mutations in mon are generally lethal by 10 to 14 d of development (Ransom et al. 1996), although rare mon homozygous mutants (approximately 1 in 500 bloodless embryos) of all tested alleles survive to adulthood.
T4980 8994-9003 NNS denotes Mutations
T4981 9011-9014 VBP denotes are
T4982 9004-9006 IN denotes in
T4983 9007-9010 NN denotes mon
T4984 9015-9024 RB denotes generally
T4985 9025-9031 JJ denotes lethal
T4986 9032-9034 IN denotes by
T4987 9035-9037 CD denotes 10
T4988 9041-9043 CD denotes 14
T4989 9038-9040 IN denotes to
T4990 9044-9045 NN denotes d
T4991 9046-9048 IN denotes of
T4992 9049-9060 NN denotes development
T4993 9061-9062 -LRB- denotes (
T4994 9062-9068 NNP denotes Ransom
T4995 9069-9071 FW denotes et
T4996 9072-9075 FW denotes al.
T4997 9076-9080 CD denotes 1996
T4998 9080-9081 -RRB- denotes )
T4999 9081-9083 , denotes ,
T5000 9083-9091 IN denotes although
T5001 9185-9192 VBP denotes survive
T5002 9092-9096 JJ denotes rare
T5003 9112-9119 NNS denotes mutants
T5004 9097-9100 NN denotes mon
T5005 9101-9111 JJ denotes homozygous
T5006 9120-9121 -LRB- denotes (
T5007 9154-9161 NNS denotes embryos
T5008 9121-9134 RB denotes approximately
T5009 9140-9143 CD denotes 500
T5010 9135-9136 CD denotes 1
T5011 9137-9139 IN denotes in
T5012 9144-9153 JJ denotes bloodless
T5013 9161-9162 -RRB- denotes )
T5014 9163-9165 IN denotes of
T5015 9166-9169 DT denotes all
T5016 9177-9184 NNS denotes alleles
T5017 9170-9176 VBN denotes tested
T5018 9193-9195 IN denotes to
T5019 9196-9205 NN denotes adulthood
T5020 9205-9206 . denotes .
T5021 9206-9329 sentence denotes Adult mon mutants show cardiac hypertrophy, presumably due to the severe anemia leading to a high output state (Figure 2).
T5022 9207-9212 JJ denotes Adult
T5023 9217-9224 NNS denotes mutants
T5024 9213-9216 NN denotes mon
T5025 9225-9229 VBP denotes show
T5026 9230-9237 JJ denotes cardiac
T5027 9238-9249 NN denotes hypertrophy
T5028 9249-9251 , denotes ,
T5029 9251-9261 RB denotes presumably
T5030 9262-9265 IN denotes due
T5031 9266-9268 IN denotes to
T5032 9269-9272 DT denotes the
T5033 9280-9286 NN denotes anemia
T5034 9273-9279 JJ denotes severe
T5035 9287-9294 VBG denotes leading
T5036 9295-9297 IN denotes to
T5037 9298-9299 DT denotes a
T5038 9312-9317 NN denotes state
T5039 9300-9304 JJ denotes high
T5040 9305-9311 NN denotes output
T5041 9318-9319 -LRB- denotes (
T5042 9319-9325 NN denotes Figure
T5043 9326-9327 CD denotes 2
T5044 9327-9328 -RRB- denotes )
T5045 9328-9329 . denotes .
T5046 9329-9546 sentence denotes In wild-type zebrafish, the adult site of hematopoiesis is the kidney (Al Adhami and Kunz 1977), which contains erythroid, lymphoid, and myeloid populations at various stages of differentiation (Bennett et al. 2001).
T5047 9330-9332 IN denotes In
T5048 9386-9388 VBZ denotes is
T5049 9333-9337 JJ denotes wild
T5050 9338-9342 NN denotes type
T5051 9337-9338 HYPH denotes -
T5052 9343-9352 NN denotes zebrafish
T5053 9352-9354 , denotes ,
T5054 9354-9357 DT denotes the
T5055 9364-9368 NN denotes site
T5056 9358-9363 JJ denotes adult
T5057 9369-9371 IN denotes of
T5058 9372-9385 NN denotes hematopoiesis
T5059 9389-9392 DT denotes the
T5060 9393-9399 NN denotes kidney
T5061 9400-9401 -LRB- denotes (
T5062 9401-9403 NNP denotes Al
T5063 9404-9410 NNP denotes Adhami
T5064 9411-9414 CC denotes and
T5065 9415-9419 NNP denotes Kunz
T5066 9420-9424 CD denotes 1977
T5067 9424-9425 -RRB- denotes )
T5068 9425-9427 , denotes ,
T5069 9427-9432 WDT denotes which
T5070 9433-9441 VBZ denotes contains
T5071 9442-9451 JJ denotes erythroid
T5072 9475-9486 NNS denotes populations
T5073 9451-9453 , denotes ,
T5074 9453-9461 JJ denotes lymphoid
T5075 9461-9463 , denotes ,
T5076 9463-9466 CC denotes and
T5077 9467-9474 JJ denotes myeloid
T5078 9487-9489 IN denotes at
T5079 9490-9497 JJ denotes various
T5080 9498-9504 NNS denotes stages
T5081 9505-9507 IN denotes of
T5082 9508-9523 NN denotes differentiation
T5083 9524-9525 -LRB- denotes (
T5084 9525-9532 NNP denotes Bennett
T5085 9533-9535 FW denotes et
T5086 9536-9539 FW denotes al.
T5087 9540-9544 CD denotes 2001
T5088 9544-9545 -RRB- denotes )
T5089 9545-9546 . denotes .
T5090 9546-9727 sentence denotes In mon homozygous mutants, there is a severe block in maturation at the proerythroblast stage (Figure 2), whereas the differentiation of myeloid cells is normal (unpublished data).
T5091 9547-9549 IN denotes In
T5092 9580-9582 VBZ denotes is
T5093 9550-9553 NN denotes mon
T5094 9554-9564 JJ denotes homozygous
T5095 9565-9572 NNS denotes mutants
T5096 9572-9574 , denotes ,
T5097 9574-9579 EX denotes there
T5098 9583-9584 DT denotes a
T5099 9592-9597 NN denotes block
T5100 9585-9591 JJ denotes severe
T5101 9598-9600 IN denotes in
T5102 9601-9611 NN denotes maturation
T5103 9612-9614 IN denotes at
T5104 9615-9618 DT denotes the
T5105 9635-9640 NN denotes stage
T5106 9619-9634 NN denotes proerythroblast
T5107 9641-9642 -LRB- denotes (
T5108 9642-9648 NN denotes Figure
T5109 9649-9650 CD denotes 2
T5110 9650-9651 -RRB- denotes )
T5111 9651-9653 , denotes ,
T5112 9653-9660 IN denotes whereas
T5113 9698-9700 VBZ denotes is
T5114 9661-9664 DT denotes the
T5115 9665-9680 NN denotes differentiation
T5116 9681-9683 IN denotes of
T5117 9684-9691 JJ denotes myeloid
T5118 9692-9697 NNS denotes cells
T5119 9701-9707 JJ denotes normal
T5120 9708-9709 -LRB- denotes (
T5121 9721-9725 NNS denotes data
T5122 9709-9720 JJ denotes unpublished
T5123 9725-9726 -RRB- denotes )
T5124 9726-9727 . denotes .
T5125 9727-9829 sentence denotes This demonstrates that the mon gene product acts during both primitive and definitive erythropoiesis.
T5126 9728-9732 DT denotes This
T5127 9733-9745 VBZ denotes demonstrates
T5128 9746-9750 IN denotes that
T5129 9772-9776 VBZ denotes acts
T5130 9751-9754 DT denotes the
T5131 9764-9771 NN denotes product
T5132 9755-9758 NN denotes mon
T5133 9759-9763 NN denotes gene
T5134 9777-9783 IN denotes during
T5135 9784-9788 CC denotes both
T5136 9789-9798 JJ denotes primitive
T5137 9814-9828 NN denotes erythropoiesis
T5138 9799-9802 CC denotes and
T5139 9803-9813 JJ denotes definitive
T5140 9828-9829 . denotes .
T8299 13919-13930 JJ denotes chromosomal
T8300 13931-13939 NN denotes location
T7892 3832-3842 JJ denotes Positional
T7893 3843-3850 NN denotes cloning
T7894 3851-3861 VBZ denotes identifies
T7895 10374-10377 NN denotes mon
T7896 10378-10380 IN denotes as
T7897 10381-10384 DT denotes the
T7898 10395-10403 NN denotes Ortholog
T7899 10385-10394 NN denotes Zebrafish
T7900 10404-10406 IN denotes of
T7901 10407-10416 JJ denotes Mammalian
T7902 10417-10422 NN denotes TIF1γ
T7903 10422-10606 sentence denotes We identified the mon gene by positional cloning using a panel of 2,200 diploid mutants collected from Tübingen background (TU)/WIK strain hybrid parents carrying the montg234 allele.
T7904 10423-10425 PRP denotes We
T7905 10426-10436 VBD denotes identified
T7906 10437-10440 DT denotes the
T7907 10445-10449 NN denotes gene
T7908 10441-10444 NN denotes mon
T7909 10450-10452 IN denotes by
T7910 10453-10463 JJ denotes positional
T7911 10464-10471 NN denotes cloning
T7912 10472-10477 VBG denotes using
T7913 10478-10479 DT denotes a
T7914 10480-10485 NN denotes panel
T7915 10486-10488 IN denotes of
T7916 10489-10494 CD denotes 2,200
T7917 10503-10510 NNS denotes mutants
T7918 10495-10502 JJ denotes diploid
T7919 10511-10520 VBN denotes collected
T7920 10521-10525 IN denotes from
T7921 10526-10534 NN denotes Tübingen
T7922 10535-10545 NN denotes background
T7923 10555-10561 NN denotes strain
T7924 10546-10547 -LRB- denotes (
T7925 10547-10549 NN denotes TU
T7926 10549-10550 -RRB- denotes )
T7927 10550-10551 HYPH denotes /
T7928 10551-10554 NN denotes WIK
T7929 10562-10568 NN denotes hybrid
T7930 10569-10576 NNS denotes parents
T7931 10577-10585 VBG denotes carrying
T7932 10586-10589 DT denotes the
T7933 10599-10605 NN denotes allele
T7934 10590-10598 NN denotes montg234
T7935 10605-10606 . denotes .
T7936 10606-10738 sentence denotes The mon mutant gene was positioned on Chromosome 8 between microsatellite markers z987 and z11001 (Figure 3A) (Knapik et al. 1998).
T7937 10607-10610 DT denotes The
T7938 10622-10626 NN denotes gene
T7939 10611-10614 NN denotes mon
T7940 10615-10621 JJ denotes mutant
T7941 10631-10641 VBN denotes positioned
T7942 10627-10630 VBD denotes was
T7943 10642-10644 IN denotes on
T7944 10645-10655 NN denotes Chromosome
T7945 10656-10657 CD denotes 8
T7946 10658-10665 IN denotes between
T7947 10666-10680 NN denotes microsatellite
T7948 10681-10688 NNS denotes markers
T7949 10689-10693 NN denotes z987
T7950 10694-10697 CC denotes and
T7951 10698-10704 NN denotes z11001
T7952 10705-10706 -LRB- denotes (
T7953 10706-10712 NN denotes Figure
T7954 10713-10715 CD denotes 3A
T7955 10715-10716 -RRB- denotes )
T7956 10717-10718 -LRB- denotes (
T7957 10718-10724 NNP denotes Knapik
T7958 10725-10727 FW denotes et
T7959 10728-10731 FW denotes al.
T7960 10732-10736 CD denotes 1998
T7961 10736-10737 -RRB- denotes )
T7962 10737-10738 . denotes .
T7963 10738-10942 sentence denotes For positional cloning purposes, over 12,000 polymorphic markers were screened using amplified fragment length polymorphism (AFLP) (Ransom and Zon 1999), and 36 markers within the interval were isolated.
T7964 10739-10742 IN denotes For
T7965 10809-10817 VBN denotes screened
T7966 10743-10753 JJ denotes positional
T7967 10762-10770 NNS denotes purposes
T7968 10754-10761 NN denotes cloning
T7969 10770-10772 , denotes ,
T7970 10772-10776 IN denotes over
T7971 10777-10783 CD denotes 12,000
T7972 10796-10803 NNS denotes markers
T7973 10784-10795 JJ denotes polymorphic
T7974 10804-10808 VBD denotes were
T7975 10818-10823 VBG denotes using
T7976 10824-10833 VBN denotes amplified
T7977 10850-10862 NN denotes polymorphism
T7978 10834-10842 NN denotes fragment
T7979 10843-10849 NN denotes length
T7980 10863-10864 -LRB- denotes (
T7981 10864-10868 NN denotes AFLP
T7982 10868-10869 -RRB- denotes )
T7983 10870-10871 -LRB- denotes (
T7984 10871-10877 NNP denotes Ransom
T7985 10878-10881 CC denotes and
T7986 10882-10885 NNP denotes Zon
T7987 10886-10890 CD denotes 1999
T7988 10890-10891 -RRB- denotes )
T7989 10891-10893 , denotes ,
T7990 10893-10896 CC denotes and
T7991 10897-10899 CD denotes 36
T7992 10900-10907 NNS denotes markers
T7993 10933-10941 VBN denotes isolated
T7994 10908-10914 IN denotes within
T7995 10915-10918 DT denotes the
T7996 10919-10927 NN denotes interval
T7997 10928-10932 VBD denotes were
T7998 10941-10942 . denotes .
T7999 10942-11072 sentence denotes One of these, MA3, was found to be 0.3 cM from the gene (Figure 3A) and was utilized as the starting point of a chromosomal walk.
T8000 10943-10946 CD denotes One
T8001 10966-10971 VBN denotes found
T8002 10947-10949 IN denotes of
T8003 10950-10955 DT denotes these
T8004 10955-10957 , denotes ,
T8005 10957-10960 NN denotes MA3
T8006 10960-10962 , denotes ,
T8007 10962-10965 VBD denotes was
T8008 10972-10974 TO denotes to
T8009 10975-10977 VB denotes be
T8010 10978-10981 CD denotes 0.3
T8011 10982-10984 NN denotes cM
T8012 10985-10989 IN denotes from
T8013 10990-10993 DT denotes the
T8014 10994-10998 NN denotes gene
T8015 10999-11000 -LRB- denotes (
T8016 11000-11006 NN denotes Figure
T8017 11007-11009 CD denotes 3A
T8018 11009-11010 -RRB- denotes )
T8019 11011-11014 CC denotes and
T8020 11015-11018 VBD denotes was
T8021 11019-11027 VBN denotes utilized
T8022 11028-11030 IN denotes as
T8023 11031-11034 DT denotes the
T8024 11044-11049 NN denotes point
T8025 11035-11043 VBG denotes starting
T8026 11050-11052 IN denotes of
T8027 11053-11054 DT denotes a
T8028 11067-11071 NN denotes walk
T8029 11055-11066 JJ denotes chromosomal
T8030 11071-11072 . denotes .
T8031 11072-11187 sentence denotes A critical P1 bacterial artificial chromosome clone (PAC), 107N19, was obtained that spanned the genetic interval.
T8032 11073-11074 DT denotes A
T8033 11119-11124 NN denotes clone
T8034 11075-11083 JJ denotes critical
T8035 11084-11086 NN denotes P1
T8036 11087-11096 JJ denotes bacterial
T8037 11097-11107 JJ denotes artificial
T8038 11108-11118 NN denotes chromosome
T8039 11144-11152 VBN denotes obtained
T8040 11125-11126 -LRB- denotes (
T8041 11126-11129 NN denotes PAC
T8042 11129-11130 -RRB- denotes )
T8043 11130-11132 , denotes ,
T8044 11132-11138 NN denotes 107N19
T8045 11138-11140 , denotes ,
T8046 11140-11143 VBD denotes was
T8047 11153-11157 WDT denotes that
T8048 11158-11165 VBD denotes spanned
T8049 11166-11169 DT denotes the
T8050 11178-11186 NN denotes interval
T8051 11170-11177 JJ denotes genetic
T8052 11186-11187 . denotes .
T8053 11187-11309 sentence denotes Two simple sequence conformation polymorphism (SSCP) markers found on this PAC clone flank the critical genetic interval.
T8054 11188-11191 CD denotes Two
T8055 11241-11248 NNS denotes markers
T8056 11192-11198 JJ denotes simple
T8057 11221-11233 NN denotes polymorphism
T8058 11199-11207 NN denotes sequence
T8059 11208-11220 NN denotes conformation
T8060 11234-11235 -LRB- denotes (
T8061 11235-11239 NN denotes SSCP
T8062 11239-11240 -RRB- denotes )
T8063 11273-11278 VBP denotes flank
T8064 11249-11254 VBN denotes found
T8065 11255-11257 IN denotes on
T8066 11258-11262 DT denotes this
T8067 11267-11272 NN denotes clone
T8068 11263-11266 NN denotes PAC
T8069 11279-11282 DT denotes the
T8070 11300-11308 NN denotes interval
T8071 11283-11291 JJ denotes critical
T8072 11292-11299 JJ denotes genetic
T8073 11308-11309 . denotes .
T8074 11309-11484 sentence denotes The marker 80M12-T7 maps two recombinants out of 4,400 meioses telomeric of the mutation, and the marker 157J23-T7 maps one recombinant centromic of the mutation (Figure 3A).
T8075 11310-11313 DT denotes The
T8076 11314-11320 NN denotes marker
T8077 11330-11334 VBZ denotes maps
T8078 11321-11326 NN denotes 80M12
T8079 11327-11329 NN denotes T7
T8080 11326-11327 HYPH denotes -
T8081 11335-11338 CD denotes two
T8082 11339-11351 NNS denotes recombinants
T8083 11352-11355 IN denotes out
T8084 11356-11358 IN denotes of
T8085 11359-11364 CD denotes 4,400
T8086 11365-11372 NNS denotes meioses
T8087 11373-11382 JJ denotes telomeric
T8088 11383-11385 IN denotes of
T8089 11386-11389 DT denotes the
T8090 11390-11398 NN denotes mutation
T8091 11398-11400 , denotes ,
T8092 11400-11403 CC denotes and
T8093 11404-11407 DT denotes the
T8094 11408-11414 NN denotes marker
T8095 11425-11429 VBZ denotes maps
T8096 11415-11421 NN denotes 157J23
T8097 11422-11424 NN denotes T7
T8098 11421-11422 HYPH denotes -
T8099 11430-11433 CD denotes one
T8100 11446-11455 NN denotes centromic
T8101 11434-11445 JJ denotes recombinant
T8102 11456-11458 IN denotes of
T8103 11459-11462 DT denotes the
T8104 11463-11471 NN denotes mutation
T8105 11472-11473 -LRB- denotes (
T8106 11473-11479 NN denotes Figure
T8107 11480-11482 CD denotes 3A
T8108 11482-11483 -RRB- denotes )
T8109 11483-11484 . denotes .
T8110 11484-11682 sentence denotes The end sequences and SSCP markers of PAC 107N19 are found in the zebrafish genomic sequence contig ctg23107 (http://www.ensembl.org/Danio_rerio/) containing a predicted zebrafish TIF1 family gene.
T8111 11485-11488 DT denotes The
T8112 11493-11502 NNS denotes sequences
T8113 11489-11492 NN denotes end
T8114 11538-11543 VBN denotes found
T8115 11503-11506 CC denotes and
T8116 11507-11511 NN denotes SSCP
T8117 11512-11519 NNS denotes markers
T8118 11520-11522 IN denotes of
T8119 11523-11526 NN denotes PAC
T8120 11527-11533 NN denotes 107N19
T8121 11534-11537 VBP denotes are
T8122 11544-11546 IN denotes in
T8123 11547-11550 DT denotes the
T8124 11578-11584 NN denotes contig
T8125 11551-11560 NN denotes zebrafish
T8126 11561-11568 JJ denotes genomic
T8127 11569-11577 NN denotes sequence
T8128 11585-11593 NN denotes ctg23107
T8129 11594-11595 -LRB- denotes (
T8130 11595-11630 NN denotes http://www.ensembl.org/Danio_rerio/
T8131 11630-11631 -RRB- denotes )
T8132 11632-11642 VBG denotes containing
T8133 11643-11644 DT denotes a
T8134 11677-11681 NN denotes gene
T8135 11645-11654 VBN denotes predicted
T8136 11655-11664 NN denotes zebrafish
T8137 11665-11669 NN denotes TIF1
T8138 11670-11676 NN denotes family
T8139 11681-11682 . denotes .
T8140 11682-11806 sentence denotes This PAC was hybridized to a kidney cDNA library, resulting in the isolation of four clones that represented the same gene.
T8141 11683-11687 DT denotes This
T8142 11688-11691 NN denotes PAC
T8143 11696-11706 VBN denotes hybridized
T8144 11692-11695 VBD denotes was
T8145 11707-11709 IN denotes to
T8146 11710-11711 DT denotes a
T8147 11724-11731 NN denotes library
T8148 11712-11718 NN denotes kidney
T8149 11719-11723 NN denotes cDNA
T8150 11731-11733 , denotes ,
T8151 11733-11742 VBG denotes resulting
T8152 11743-11745 IN denotes in
T8153 11746-11749 DT denotes the
T8154 11750-11759 NN denotes isolation
T8155 11760-11762 IN denotes of
T8156 11763-11767 CD denotes four
T8157 11768-11774 NNS denotes clones
T8158 11775-11779 WDT denotes that
T8159 11780-11791 VBD denotes represented
T8160 11792-11795 DT denotes the
T8161 11801-11805 NN denotes gene
T8162 11796-11800 JJ denotes same
T8163 11805-11806 . denotes .
T8164 11806-13342 sentence denotes Figure 3 Positional Cloning Identifies the mon Gene as Zebrafish tif1γ (A) Physical map of the mon locus on zebrafish Chromosome 8. Microsatellite markers z987 and z11001 were used to initially identify recombinants in a panel of 2,200 diploid montg234 homozygous mutants. The AFLP marker MA3 was used to initiate a chromosomal walk in PAC libraries. The critical PACS that were isolated to encompass the mon locus are indicated by numbers above bar. The PAC 107N19 defines the critical interval for the mon gene. This PAC was used as a probe to screen cDNA libraries and to identify zebrafish tif1γ cDNAs. Numbers below the bar indicate the number of recombinants identified by SSCP analysis. (B) Clustal-W–generated phylogentic tree of zebrafish (Danio rerio [Dr]) Tif1γ and Tif1α peptide sequences in comparison to TIF1 family members: human (Hs) TIF1α, TIF1β, and TIF1γ; mouse (Mm) Tif1α, Tif1β, and Tif1γ;; and fly (Dm) bonus. (C) Diagrams illustrating the structure of the Tif1γ-predicted peptide and the three identified point mutants. RING finger (RING), B-boxes (B1 and B2), plant homeodomain finger (PHD) and bromodomain (BROMO). Numbers below the first diagram indicate the percent identity shared between each of these domains in zebrafish and human TIF1γ. The predicted truncated proteins are indicated. (D) DNA sequence chromatograms showing the three ENU-induced point mutants in comparison to wild-type control sequences The mon gene encodes a member of the TIF1 family of transcriptional cofactors (Figure 3B and 3C).
T8165 13245-13248 DT denotes The
T8166 13253-13257 NN denotes gene
T8167 13249-13252 NN denotes mon
T8168 13258-13265 VBZ denotes encodes
T8169 13266-13267 DT denotes a
T8170 13268-13274 NN denotes member
T8171 13275-13277 IN denotes of
T8172 13278-13281 DT denotes the
T8173 13287-13293 NN denotes family
T8174 13282-13286 NN denotes TIF1
T8175 13294-13296 IN denotes of
T8176 13297-13312 JJ denotes transcriptional
T8177 13313-13322 NNS denotes cofactors
T8178 13323-13324 -LRB- denotes (
T8179 13324-13330 NN denotes Figure
T8180 13331-13333 CD denotes 3B
T8181 13334-13337 CC denotes and
T8182 13338-13340 CD denotes 3C
T8183 13340-13341 -RRB- denotes )
T8184 13341-13342 . denotes .
T8185 13342-13579 sentence denotes The coding sequence of mon is most similar to human TIFγ (Le Douarin et al. 1995; Friedman et al. 1996; Venturini et al. 1999), and the locations of exon boundaries are conserved between the zebrafish and human genes (unpublished data).
T8186 13343-13346 DT denotes The
T8187 13354-13362 NN denotes sequence
T8188 13347-13353 VBG denotes coding
T8189 13370-13372 VBZ denotes is
T8190 13363-13365 IN denotes of
T8191 13366-13369 NN denotes mon
T8192 13373-13377 RBS denotes most
T8193 13378-13385 JJ denotes similar
T8194 13386-13388 IN denotes to
T8195 13389-13394 JJ denotes human
T8196 13395-13399 NN denotes TIFγ
T8197 13400-13401 -LRB- denotes (
T8198 13401-13403 NNP denotes Le
T8199 13404-13411 NNP denotes Douarin
T8200 13412-13414 FW denotes et
T8201 13415-13418 FW denotes al.
T8202 13419-13423 CD denotes 1995
T8203 13423-13424 : denotes ;
T8204 13425-13433 NNP denotes Friedman
T8205 13434-13436 FW denotes et
T8206 13437-13440 FW denotes al.
T8207 13441-13445 CD denotes 1996
T8208 13445-13446 : denotes ;
T8209 13447-13456 NNP denotes Venturini
T8210 13457-13459 FW denotes et
T8211 13460-13463 FW denotes al.
T8212 13464-13468 CD denotes 1999
T8213 13468-13469 -RRB- denotes )
T8214 13469-13471 , denotes ,
T8215 13471-13474 CC denotes and
T8216 13475-13478 DT denotes the
T8217 13479-13488 NNS denotes locations
T8218 13512-13521 VBN denotes conserved
T8219 13489-13491 IN denotes of
T8220 13492-13496 NN denotes exon
T8221 13497-13507 NNS denotes boundaries
T8222 13508-13511 VBP denotes are
T8223 13522-13529 IN denotes between
T8224 13530-13533 DT denotes the
T8225 13554-13559 NNS denotes genes
T8226 13534-13543 NN denotes zebrafish
T8227 13544-13547 CC denotes and
T8228 13548-13553 JJ denotes human
T8229 13560-13561 -LRB- denotes (
T8230 13573-13577 NNS denotes data
T8231 13561-13572 JJ denotes unpublished
T8232 13577-13578 -RRB- denotes )
T8233 13578-13579 . denotes .
T8234 13579-13874 sentence denotes The mon locus on zebrafish Chromosome 8 is also predicted to be syntenic to the region of human Chromosome 1p that contains the TIF1γ gene based on the conserved locations of 12 other orthologous gene pairs, including NRAS, mapped to these regions in human and zebrafish (Barbazuk et al. 2000).
T8235 13580-13583 DT denotes The
T8236 13588-13593 NN denotes locus
T8237 13584-13587 NN denotes mon
T8238 13628-13637 VBN denotes predicted
T8239 13594-13596 IN denotes on
T8240 13597-13606 NN denotes zebrafish
T8241 13607-13617 NN denotes Chromosome
T8242 13618-13619 CD denotes 8
T8243 13620-13622 VBZ denotes is
T8244 13623-13627 RB denotes also
T8245 13638-13640 TO denotes to
T8246 13641-13643 VB denotes be
T8247 13644-13652 JJ denotes syntenic
T8248 13653-13655 IN denotes to
T8249 13656-13659 DT denotes the
T8250 13660-13666 NN denotes region
T8251 13667-13669 IN denotes of
T8252 13670-13675 JJ denotes human
T8253 13676-13686 NN denotes Chromosome
T8254 13687-13689 CD denotes 1p
T8255 13690-13694 WDT denotes that
T8256 13695-13703 VBZ denotes contains
T8257 13704-13707 DT denotes the
T8258 13714-13718 NN denotes gene
T8259 13708-13713 NN denotes TIF1γ
T8260 13719-13724 VBN denotes based
T8261 13725-13727 IN denotes on
T8262 13728-13731 DT denotes the
T8263 13742-13751 NNS denotes locations
T8264 13732-13741 VBN denotes conserved
T8265 13752-13754 IN denotes of
T8266 13755-13757 CD denotes 12
T8267 13781-13786 NNS denotes pairs
T8268 13758-13763 JJ denotes other
T8269 13764-13775 JJ denotes orthologous
T8270 13776-13780 NN denotes gene
T8271 13786-13788 , denotes ,
T8272 13788-13797 VBG denotes including
T8273 13798-13802 NN denotes NRAS
T8274 13802-13804 , denotes ,
T8275 13804-13810 VBN denotes mapped
T8276 13811-13813 IN denotes to
T8277 13814-13819 DT denotes these
T8278 13820-13827 NNS denotes regions
T8279 13828-13830 IN denotes in
T8280 13831-13836 JJ denotes human
T8301 13939-13941 , denotes ,
T8302 13941-13944 DT denotes the
T8303 13959-13963 NN denotes gene
T8304 13945-13954 NN denotes zebrafish
T8305 13955-13958 NN denotes mon
T8306 13967-13970 DT denotes the
T8307 13978-13986 NN denotes ortholog
T8308 13971-13977 JJ denotes likely
T8309 13987-13989 IN denotes of
T8310 13990-13993 DT denotes the
T8311 14006-14010 NN denotes gene
T8312 13994-13999 JJ denotes human
T8313 14000-14005 NN denotes TIF1γ
T8314 14010-14011 . denotes .
T8315 14011-14170 sentence denotes We have identified ethyl-nitrosourea (ENU)-induced point mutations in three alleles of mon (Figure 3C and 3D), each of which generates a premature stop codon.
T8316 14012-14014 PRP denotes We
T8317 14020-14030 VBN denotes identified
T8318 14015-14019 VBP denotes have
T8319 14031-14036 NN denotes ethyl
T8320 14037-14048 NN denotes nitrosourea
T8321 14036-14037 HYPH denotes -
T8322 14055-14062 VBN denotes induced
T8323 14049-14050 -LRB- denotes (
T8324 14050-14053 NN denotes ENU
T8325 14053-14054 -RRB- denotes )
T8326 14054-14055 HYPH denotes -
T8327 14069-14078 NNS denotes mutations
T8328 14063-14068 NN denotes point
T8329 14079-14081 IN denotes in
T8330 14082-14087 CD denotes three
T8331 14088-14095 NNS denotes alleles
T8332 14096-14098 IN denotes of
T8333 14099-14102 NN denotes mon
T8334 14103-14104 -LRB- denotes (
T8335 14111-14113 CD denotes 3C
T8336 14104-14110 NN denotes Figure
T8337 14114-14117 CC denotes and
T8338 14118-14120 CD denotes 3D
T8339 14120-14121 -RRB- denotes )
T8340 14121-14123 , denotes ,
T8341 14123-14127 DT denotes each
T8342 14137-14146 VBZ denotes generates
T8343 14128-14130 IN denotes of
T8344 14131-14136 WDT denotes which
T8345 14147-14148 DT denotes a
T8346 14164-14169 NN denotes codon
T8347 14149-14158 JJ denotes premature
T8348 14159-14163 NN denotes stop
T8349 14169-14170 . denotes .
T8350 14170-14262 sentence denotes The montb222b and montg234 alleles have a severe phenotype with no circulating blood cells.
T8351 14171-14174 DT denotes The
T8352 14198-14205 NNS denotes alleles
T8353 14175-14184 NN denotes montb222b
T8354 14185-14188 CC denotes and
T8355 14189-14197 NN denotes montg234
T8356 14206-14210 VBP denotes have
T8357 14211-14212 DT denotes a
T8358 14220-14229 NN denotes phenotype
T8359 14213-14219 JJ denotes severe
T8360 14230-14234 IN denotes with
T8361 14235-14237 DT denotes no
T8362 14256-14261 NNS denotes cells
T8363 14238-14249 VBG denotes circulating
T8364 14250-14255 NN denotes blood
T8365 14261-14262 . denotes .
T8366 14262-14466 sentence denotes In contrast, the monm262 allele has 10–100 circulating blood cells by 48 hpf, in comparison to the approximately 3,000 RBCs in the circulation of wild-type or heterozygous embryos at the same time point.
T8367 14263-14265 IN denotes In
T8368 14295-14298 VBZ denotes has
T8369 14266-14274 NN denotes contrast
T8370 14274-14276 , denotes ,
T8371 14276-14279 DT denotes the
T8372 14288-14294 NN denotes allele
T8373 14280-14287 NN denotes monm262
T8374 14299-14301 CD denotes 10
T8375 14302-14305 CD denotes 100
T8376 14301-14302 SYM denotes –
T8377 14324-14329 NNS denotes cells
T8378 14306-14317 VBG denotes circulating
T8379 14318-14323 NN denotes blood
T8380 14330-14332 IN denotes by
T8381 14333-14335 CD denotes 48
T8382 14336-14339 NN denotes hpf
T8383 14339-14341 , denotes ,
T8384 14341-14343 IN denotes in
T8385 14344-14354 NN denotes comparison
T8386 14355-14357 IN denotes to
T8387 14358-14361 DT denotes the
T8388 14382-14386 NNS denotes RBCs
T8389 14362-14375 RB denotes approximately
T8390 14376-14381 CD denotes 3,000
T8391 14387-14389 IN denotes in
T8392 14390-14393 DT denotes the
T8393 14394-14405 NN denotes circulation
T8394 14406-14408 IN denotes of
T8395 14409-14413 JJ denotes wild
T8396 14414-14418 NN denotes type
T8397 14413-14414 HYPH denotes -
T8398 14435-14442 NNS denotes embryos
T8399 14419-14421 CC denotes or
T8400 14422-14434 JJ denotes heterozygous
T8401 14443-14445 IN denotes at
T8402 14446-14449 DT denotes the
T8403 14460-14465 NN denotes point
T8404 14450-14454 JJ denotes same
T8405 14455-14459 NN denotes time
T8406 14465-14466 . denotes .
T8407 14466-14607 sentence denotes The monm262 allele was found to encode a premature stop codon at position E40, which would encode a putative protein of only 40 amino acids.
T8408 14467-14470 DT denotes The
T8409 14479-14485 NN denotes allele
T8410 14471-14478 NN denotes monm262
T8411 14490-14495 VBN denotes found
T8412 14486-14489 VBD denotes was
T8413 14496-14498 TO denotes to
T8414 14499-14505 VB denotes encode
T8415 14506-14507 DT denotes a
T8416 14523-14528 NN denotes codon
T8417 14508-14517 JJ denotes premature
T8418 14518-14522 NN denotes stop
T8419 14529-14531 IN denotes at
T8420 14532-14540 NN denotes position
T8421 14541-14544 NN denotes E40
T8422 14544-14546 , denotes ,
T8423 14546-14551 WDT denotes which
T8424 14558-14564 VB denotes encode
T8425 14552-14557 MD denotes would
T8426 14565-14566 DT denotes a
T8427 14576-14583 NN denotes protein
T8428 14567-14575 JJ denotes putative
T8429 14584-14586 IN denotes of
T8430 14587-14591 RB denotes only
T8431 14601-14606 NNS denotes acids
T8432 14592-14594 CD denotes 40
T8433 14595-14600 NN denotes amino
T8434 14606-14607 . denotes .
T8435 14607-14763 sentence denotes Although this mutation would be expected to lead to a complete loss of mon gene product, another methionine is found downstream at amino acid position 104.
T8436 14608-14616 IN denotes Although
T8437 14640-14648 VBN denotes expected
T8438 14617-14621 DT denotes this
T8439 14622-14630 NN denotes mutation
T8440 14631-14636 MD denotes would
T8441 14637-14639 VB denotes be
T8442 14719-14724 VBN denotes found
T8443 14649-14651 TO denotes to
T8444 14652-14656 VB denotes lead
T8445 14657-14659 IN denotes to
T8446 14660-14661 DT denotes a
T8447 14671-14675 NN denotes loss
T8448 14662-14670 JJ denotes complete
T8449 14676-14678 IN denotes of
T8450 14679-14682 NN denotes mon
T8451 14683-14687 NN denotes gene
T8452 14688-14695 NN denotes product
T8453 14695-14697 , denotes ,
T8454 14697-14704 DT denotes another
T8455 14705-14715 NN denotes methionine
T8456 14716-14718 VBZ denotes is
T8457 14725-14735 RB denotes downstream
T8458 14736-14738 IN denotes at
T8459 14739-14744 NN denotes amino
T8460 14745-14749 NN denotes acid
T8461 14750-14758 NN denotes position
T8462 14759-14762 CD denotes 104
T8463 14762-14763 . denotes .
T8464 14763-14901 sentence denotes In vitro translation experiments in reticulocyte lysates demonstrate reinitiation of translation from this methionine (unpublished data).
T8465 14764-14766 FW denotes In
T8466 14767-14772 FW denotes vitro
T8467 14785-14796 NNS denotes experiments
T8468 14773-14784 NN denotes translation
T8469 14821-14832 VBP denotes demonstrate
T8470 14797-14799 IN denotes in
T8471 14800-14812 NN denotes reticulocyte
T8472 14813-14820 NNS denotes lysates
T8473 14833-14845 NN denotes reinitiation
T8474 14846-14848 IN denotes of
T8475 14849-14860 NN denotes translation
T8476 14861-14865 IN denotes from
T8477 14866-14870 DT denotes this
T8478 14871-14881 NN denotes methionine
T8479 14882-14883 -LRB- denotes (
T8480 14895-14899 NNS denotes data
T8481 14883-14894 VBN denotes unpublished
T8482 14899-14900 -RRB- denotes )
T8483 14900-14901 . denotes .
T8484 14901-15028 sentence denotes Therefore, the hypomorphic larval phenotype of the monm262 allele is likely due to partial loss of mon function or expression.
T8485 14902-14911 RB denotes Therefore
T8486 14968-14970 VBZ denotes is
T8487 14911-14913 , denotes ,
T8488 14913-14916 DT denotes the
T8489 14936-14945 NN denotes phenotype
T8490 14917-14928 JJ denotes hypomorphic
T8491 14929-14935 JJ denotes larval
T8492 14946-14948 IN denotes of
T8493 14949-14952 DT denotes the
T8494 14961-14967 NN denotes allele
T8495 14953-14960 NN denotes monm262
T8496 14971-14977 RB denotes likely
T8497 14978-14981 IN denotes due
T8498 14982-14984 IN denotes to
T8499 14985-14992 JJ denotes partial
T8500 14993-14997 NN denotes loss
T8501 14998-15000 IN denotes of
T8502 15001-15004 NN denotes mon
T8503 15005-15013 NN denotes function
T8504 15014-15016 CC denotes or
T8505 15017-15027 NN denotes expression
T8506 15027-15028 . denotes .
T8507 15028-15156 sentence denotes The presence of mutations in each of the mon alleles indicates that defective Tif1γ function is the cause of the mon phenotype.
T8508 15029-15032 DT denotes The
T8509 15033-15041 NN denotes presence
T8510 15082-15091 VBZ denotes indicates
T8511 15042-15044 IN denotes of
T8512 15045-15054 NNS denotes mutations
T8513 15055-15057 IN denotes in
T8514 15058-15062 DT denotes each
T8515 15063-15065 IN denotes of
T8516 15066-15069 DT denotes the
T8517 15074-15081 NNS denotes alleles
T8518 15070-15073 NN denotes mon
T8519 15092-15096 IN denotes that
T8520 15122-15124 VBZ denotes is
T8521 15097-15106 JJ denotes defective
T8522 15113-15121 NN denotes function
T8523 15107-15112 NN denotes Tif1γ
T8524 15125-15128 DT denotes the
T8525 15129-15134 NN denotes cause
T8526 15135-15137 IN denotes of
T8527 15138-15141 DT denotes the
T8528 15146-15155 NN denotes phenotype
T8529 15142-15145 NN denotes mon
T8530 15155-15156 . denotes .
T8531 15156-15317 sentence denotes In order to determine whether tif1γ is expressed in hematopoietic mesoderm, we next examined zebrafish embryos by whole-mount in situ hybridization (Figure 4A).
T8532 15157-15159 IN denotes In
T8533 15241-15249 VBD denotes examined
T8534 15160-15165 NN denotes order
T8535 15166-15168 TO denotes to
T8536 15169-15178 VB denotes determine
T8537 15179-15186 IN denotes whether
T8538 15196-15205 VBN denotes expressed
T8539 15187-15192 NN denotes tif1γ
T8540 15193-15195 VBZ denotes is
T8541 15206-15208 IN denotes in
T8542 15209-15222 JJ denotes hematopoietic
T8543 15223-15231 NN denotes mesoderm
T8544 15231-15233 , denotes ,
T8545 15233-15235 PRP denotes we
T8546 15236-15240 RB denotes next
T8547 15250-15259 NN denotes zebrafish
T8548 15260-15267 NNS denotes embryos
T8549 15268-15270 IN denotes by
T8550 15271-15276 JJ denotes whole
T8551 15277-15282 NN denotes mount
T8552 15276-15277 HYPH denotes -
T8553 15291-15304 NN denotes hybridization
T8554 15283-15285 FW denotes in
T8555 15286-15290 FW denotes situ
T8556 15305-15306 -LRB- denotes (
T8557 15306-15312 NN denotes Figure
T8558 15313-15315 CD denotes 4A
T8559 15315-15316 -RRB- denotes )
T8560 15316-15317 . denotes .
T8561 15317-15411 sentence denotes tif1γ mRNA is expressed maternally and is found throughout the embryo during blastula stages.
T8562 15318-15323 NN denotes tif1γ
T8563 15324-15328 NN denotes mRNA
T8564 15332-15341 VBN denotes expressed
T8565 15329-15331 VBZ denotes is
T8566 15342-15352 RB denotes maternally
T8567 15353-15356 CC denotes and
T8568 15357-15359 VBZ denotes is
T8569 15360-15365 VBN denotes found
T8570 15366-15376 IN denotes throughout
T8571 15377-15380 DT denotes the
T8572 15381-15387 NN denotes embryo
T8573 15388-15394 IN denotes during
T8574 15395-15403 NN denotes blastula
T8575 15404-15410 NNS denotes stages
T8576 15410-15411 . denotes .
T8577 15411-15525 sentence denotes During gastrulation and epiboly stages, zygotic expression of mon is highest in the mesendoderm of the germ ring.
T8578 15412-15418 IN denotes During
T8579 15478-15480 VBZ denotes is
T8580 15419-15431 NN denotes gastrulation
T8581 15444-15450 NNS denotes stages
T8582 15432-15435 CC denotes and
T8583 15436-15443 NN denotes epiboly
T8584 15450-15452 , denotes ,
T8585 15452-15459 JJ denotes zygotic
T8586 15460-15470 NN denotes expression
T8587 15471-15473 IN denotes of
T8588 15474-15477 NN denotes mon
T8589 15481-15488 JJS denotes highest
T8590 15489-15491 IN denotes in
T8591 15492-15495 DT denotes the
T8592 15496-15507 NN denotes mesendoderm
T8593 15508-15510 IN denotes of
T8594 15511-15514 DT denotes the
T8595 15520-15524 NN denotes ring
T8596 15515-15519 NN denotes germ
T8597 15524-15525 . denotes .
T8598 15525-15790 sentence denotes At tail bud and early somite stages a high level of tif1γ expression delineates a horseshoe-shaped population of ventral/lateral mesoderm that will give rise to blood and also expresses stem cell leukemiahematopoietic transcription factor (scl) (Liao et al. 1997).
T8599 15526-15528 IN denotes At
T8600 15595-15605 VBZ denotes delineates
T8601 15529-15533 NN denotes tail
T8602 15534-15537 NN denotes bud
T8603 15555-15561 NNS denotes stages
T8604 15538-15541 CC denotes and
T8605 15542-15547 JJ denotes early
T8606 15548-15554 NN denotes somite
T8607 15562-15563 DT denotes a
T8608 15569-15574 NN denotes level
T8609 15564-15568 JJ denotes high
T8610 15575-15577 IN denotes of
T8611 15578-15583 NN denotes tif1γ
T8612 15584-15594 NN denotes expression
T8613 15606-15607 DT denotes a
T8614 15625-15635 NN denotes population
T8615 15608-15617 NN denotes horseshoe
T8616 15618-15624 VBN denotes shaped
T8617 15617-15618 HYPH denotes -
T8618 15636-15638 IN denotes of
T8619 15639-15646 JJ denotes ventral
T8620 15647-15654 JJ denotes lateral
T8621 15646-15647 HYPH denotes /
T8622 15655-15663 NN denotes mesoderm
T8623 15664-15668 WDT denotes that
T8624 15674-15678 VB denotes give
T8625 15669-15673 MD denotes will
T8626 15679-15683 NN denotes rise
T8627 15684-15686 IN denotes to
T8628 15687-15692 NN denotes blood
T8629 15693-15696 CC denotes and
T8630 15697-15701 RB denotes also
T8631 15702-15711 VBZ denotes expresses
T8632 15712-15716 NN denotes stem
T8633 15717-15721 NN denotes cell
T8634 15758-15764 NN denotes factor
T8635 15722-15743 JJ denotes leukemiahematopoietic
T8636 15744-15757 NN denotes transcription
T8637 15765-15766 -LRB- denotes (
T8638 15766-15769 NN denotes scl
T8639 15769-15770 -RRB- denotes )
T8640 15771-15772 -LRB- denotes (
T8641 15772-15776 NNP denotes Liao
T8642 15777-15779 FW denotes et
T8643 15780-15783 FW denotes al.
T8644 15784-15788 CD denotes 1997
T8645 15788-15789 -RRB- denotes )
T8646 15789-15790 . denotes .
T8647 15790-15924 sentence denotes This group of cells continues to express tif1γ and scl while it converges and forms the embryonic blood island (Detrich et al. 1995).
T8648 15791-15795 DT denotes This
T8649 15796-15801 NN denotes group
T8650 15811-15820 VBZ denotes continues
T8651 15802-15804 IN denotes of
T8652 15805-15810 NNS denotes cells
T8653 15821-15823 TO denotes to
T8654 15824-15831 VB denotes express
T8655 15832-15837 NN denotes tif1γ
T8656 15838-15841 CC denotes and
T8657 15842-15845 NN denotes scl
T8658 15846-15851 IN denotes while
T8659 15855-15864 VBZ denotes converges
T8660 15852-15854 PRP denotes it
T8661 15865-15868 CC denotes and
T8662 15869-15874 VBZ denotes forms
T8663 15875-15878 DT denotes the
T8664 15895-15901 NN denotes island
T8665 15879-15888 JJ denotes embryonic
T8666 15889-15894 NN denotes blood
T8667 15902-15903 -LRB- denotes (
T8668 15903-15910 NNP denotes Detrich
T8669 15911-15913 FW denotes et
T8670 15914-15917 FW denotes al.
T8671 15918-15922 CD denotes 1995
T8672 15922-15923 -RRB- denotes )
T8673 15923-15924 . denotes .
T8674 15924-16043 sentence denotes The tif1γ gene is also highly expressed in the central nervous system as well as the mesenchyme of the trunk and tail.
T8675 15925-15928 DT denotes The
T8676 15935-15939 NN denotes gene
T8677 15929-15934 NN denotes tif1γ
T8678 15955-15964 VBN denotes expressed
T8679 15940-15942 VBZ denotes is
T8680 15943-15947 RB denotes also
T8681 15948-15954 RB denotes highly
T8682 15965-15967 IN denotes in
T8683 15968-15971 DT denotes the
T8684 15988-15994 NN denotes system
T8685 15972-15979 JJ denotes central
T8686 15980-15987 JJ denotes nervous
T8687 15995-15997 RB denotes as
T8688 16003-16005 IN denotes as
T8689 15998-16002 RB denotes well
T8690 16006-16009 DT denotes the
T8691 16010-16020 NN denotes mesenchyme
T8692 16021-16023 IN denotes of
T8693 16024-16027 DT denotes the
T8694 16028-16033 NN denotes trunk
T8695 16034-16037 CC denotes and
T8696 16038-16042 NN denotes tail
T8697 16042-16043 . denotes .
T8698 16043-16179 sentence denotes Homozygous montg234 mutants have a greatly reduced amount of tif1γ mRNA in all tissues consistent with nonsense-mediated message decay.
T8699 16044-16054 JJ denotes Homozygous
T8700 16064-16071 NNS denotes mutants
T8701 16055-16063 NN denotes montg234
T8702 16072-16076 VBP denotes have
T8703 16077-16078 DT denotes a
T8704 16095-16101 NN denotes amount
T8705 16079-16086 RB denotes greatly
T8706 16087-16094 VBN denotes reduced
T8707 16102-16104 IN denotes of
T8708 16105-16110 NN denotes tif1γ
T8709 16111-16115 NN denotes mRNA
T8710 16116-16118 IN denotes in
T8711 16119-16122 DT denotes all
T8712 16123-16130 NNS denotes tissues
T8713 16131-16141 JJ denotes consistent
T8714 16142-16146 IN denotes with
T8715 16147-16155 NN denotes nonsense
T8716 16156-16164 VBN denotes mediated
T8717 16155-16156 HYPH denotes -
T8718 16173-16178 NN denotes decay
T8719 16165-16172 NN denotes message
T8720 16178-16179 . denotes .
T8721 16179-16388 sentence denotes Thus, zebrafish tif1γ is specifically expressed in ventral mesoderm and putative hemangioblasts prior to and during the embryonic stages when hematopoietic progenitors are undergoing apoptosis in mon mutants.
T8722 16180-16184 RB denotes Thus
T8723 16218-16227 VBN denotes expressed
T8724 16184-16186 , denotes ,
T8725 16186-16195 NN denotes zebrafish
T8726 16196-16201 NN denotes tif1γ
T8727 16202-16204 VBZ denotes is
T8728 16205-16217 RB denotes specifically
T8729 16228-16230 IN denotes in
T8730 16231-16238 JJ denotes ventral
T8731 16239-16247 NN denotes mesoderm
T8732 16248-16251 CC denotes and
T8733 16252-16260 JJ denotes putative
T8734 16261-16275 NNS denotes hemangioblasts
T8735 16276-16281 IN denotes prior
T8736 16282-16284 IN denotes to
T8737 16285-16288 CC denotes and
T8738 16289-16295 IN denotes during
T8739 16296-16299 DT denotes the
T8740 16310-16316 NNS denotes stages
T8741 16300-16309 JJ denotes embryonic
T8742 16317-16321 WRB denotes when
T8743 16352-16362 VBG denotes undergoing
T8744 16322-16335 JJ denotes hematopoietic
T8745 16336-16347 NNS denotes progenitors
T8746 16348-16351 VBP denotes are
T8747 16363-16372 NN denotes apoptosis
T8748 16373-16375 IN denotes in
T8749 16376-16379 NN denotes mon
T8750 16380-16387 NNS denotes mutants
T8751 16387-16388 . denotes .
T8752 16388-16472 sentence denotes We also compared the expression of zebrafish mon to mouse Tif1γ (Figure 4A and 4B).
T8753 16389-16391 PRP denotes We
T8754 16397-16405 VBD denotes compared
T8755 16392-16396 RB denotes also
T8756 16406-16409 DT denotes the
T8757 16410-16420 NN denotes expression
T8758 16421-16423 IN denotes of
T8759 16424-16433 NN denotes zebrafish
T8760 16434-16437 NN denotes mon
T8761 16438-16440 IN denotes to
T8762 16441-16446 NN denotes mouse
T8763 16447-16452 NN denotes Tif1γ
T8764 16453-16454 -LRB- denotes (
T8765 16461-16463 CD denotes 4A
T8766 16454-16460 NN denotes Figure
T8767 16464-16467 CC denotes and
T8768 16468-16470 CD denotes 4B
T8769 16470-16471 -RRB- denotes )
T8770 16471-16472 . denotes .
T8771 16472-16677 sentence denotes Mouse Tif1γ is highly expressed in erythroid blood islands of the yolk sac, and it is subsequently expressed in the fetal liver at a high level, and in other tissues, including the central nervous system.
T8772 16473-16478 NN denotes Mouse
T8773 16479-16484 NN denotes Tif1γ
T8774 16495-16504 VBN denotes expressed
T8775 16485-16487 VBZ denotes is
T8776 16488-16494 RB denotes highly
T8777 16505-16507 IN denotes in
T8778 16508-16517 JJ denotes erythroid
T8779 16524-16531 NNS denotes islands
T8780 16518-16523 NN denotes blood
T8781 16532-16534 IN denotes of
T8782 16535-16538 DT denotes the
T8783 16544-16547 NN denotes sac
T8784 16539-16543 NN denotes yolk
T8785 16547-16549 , denotes ,
T8786 16549-16552 CC denotes and
T8787 16553-16555 PRP denotes it
T8788 16572-16581 VBN denotes expressed
T8789 16556-16558 VBZ denotes is
T8790 16559-16571 RB denotes subsequently
T8791 16582-16584 IN denotes in
T8792 16585-16588 DT denotes the
T8793 16595-16600 NN denotes liver
T8794 16589-16594 JJ denotes fetal
T8795 16601-16603 IN denotes at
T8796 16604-16605 DT denotes a
T8797 16611-16616 NN denotes level
T8798 16606-16610 JJ denotes high
T8799 16616-16618 , denotes ,
T8800 16618-16621 CC denotes and
T8801 16622-16624 IN denotes in
T8802 16625-16630 JJ denotes other
T8803 16631-16638 NNS denotes tissues
T8804 16638-16640 , denotes ,
T8805 16640-16649 VBG denotes including
T8806 16650-16653 DT denotes the
T8807 16670-16676 NN denotes system
T8808 16654-16661 JJ denotes central
T8809 16662-16669 JJ denotes nervous
T8810 16676-16677 . denotes .
T8811 16677-16808 sentence denotes Taken together these results strongly suggest that zebrafish mon and mouse Tif1γ are orthologs that function during hematopoiesis.
T8812 16678-16683 VBN denotes Taken
T8813 16716-16723 VBP denotes suggest
T8814 16684-16692 RB denotes together
T8815 16693-16698 DT denotes these
T8816 16699-16706 NNS denotes results
T8817 16707-16715 RB denotes strongly
T8818 16724-16728 IN denotes that
T8819 16759-16762 VBP denotes are
T8820 16729-16738 NN denotes zebrafish
T8821 16739-16742 NN denotes mon
T8822 16753-16758 NN denotes Tif1γ
T8823 16743-16746 CC denotes and
T8824 16747-16752 NN denotes mouse
T8825 16763-16772 NNS denotes orthologs
T8826 16773-16777 WDT denotes that
T8827 16778-16786 VBP denotes function
T8828 16787-16793 IN denotes during
T8829 16794-16807 NN denotes hematopoiesis
T8830 16807-16808 . denotes .
T8831 16808-18302 sentence denotes Figure 4 The mon/tif1γ Gene Is Highly Expressed in Hematopoietic Mesoderm (A) In situ hybridization of zebrafish embryos demonstrating the embryonic expression of tif1γ. tif1γ is initially expressed as a maternal mRNA. Increased expression of tif1γ in ventral-lateral mesoderm begins between the one- to three-somite stages and increases through early development. By five somites, tif1γ is strongly expressed in lateral stripes of mesoderm that also express scl. At 22 hpf tif1γ is expressed broadly in the brain, spinal cord, trunk, and tail mesenchyme, but is at much higher levels in hematopoietic cells of the blood island. Zebrafish tif1α is also broadly expressed but relatively more uniform in most tissues, in comparison to tif1γ. Tif1α is weakly expressed at early somite stages in hematopoietic mesoderm and uniformly expressed at 22 hpf, including expression in the blood islands. Expression of scl at five somites and 22 hpf highlights the embryonic blood island in comparison to tif1γ expression. (B) In situ hybridization of mouse embryos detects broad expression of Tif1γ at embryonic day 8.5 including the yolk sac blood islands (arrow). AT embryonic day 12.5, there is high level expression in the fetal liver (arrow) and broad expression in the embryonic brain, spinal chord, gut, and muscle. Given that mammalian TIF1γ has been shown to form hetero-oligomers with Tif1α (Peng et al. 2002), we searched for additional TIF1 family members in zebrafish to compare with tif1γ.
T8832 18122-18127 VBN denotes Given
T8833 18223-18231 VBD denotes searched
T8834 18128-18132 IN denotes that
T8835 18158-18163 VBN denotes shown
T8836 18133-18142 JJ denotes mammalian
T8837 18143-18148 NN denotes TIF1γ
T8838 18149-18152 VBZ denotes has
T8839 18153-18157 VBN denotes been
T8840 18164-18166 TO denotes to
T8841 18167-18171 VB denotes form
T8842 18172-18188 NNS denotes hetero-oligomers
T8843 18189-18193 IN denotes with
T8844 18194-18199 NN denotes Tif1α
T8845 18200-18201 -LRB- denotes (
T8846 18201-18205 NNP denotes Peng
T8847 18206-18208 FW denotes et
T8848 18209-18212 FW denotes al.
T8849 18213-18217 CD denotes 2002
T8850 18217-18218 -RRB- denotes )
T8851 18218-18220 , denotes ,
T8852 18220-18222 PRP denotes we
T8853 18232-18235 IN denotes for
T8854 18236-18246 JJ denotes additional
T8855 18259-18266 NNS denotes members
T8856 18247-18251 NN denotes TIF1
T8857 18252-18258 NN denotes family
T8858 18267-18269 IN denotes in
T8859 18270-18279 NN denotes zebrafish
T8860 18280-18282 TO denotes to
T8861 18283-18290 VB denotes compare
T8862 18291-18295 IN denotes with
T8863 18296-18301 NN denotes tif1γ
T8864 18301-18302 . denotes .
T8865 18302-18454 sentence denotes Using zebrafish expressed sequence tag (EST) sequences, we designed primers to RT-PCR amplify a TIF1-related cDNA from embryonic 10-hpf and 24-hpf RNA.
T8866 18303-18308 VBG denotes Using
T8867 18362-18370 VBD denotes designed
T8868 18309-18318 NN denotes zebrafish
T8869 18348-18357 NNS denotes sequences
T8870 18319-18328 VBD denotes expressed
T8871 18338-18341 NN denotes tag
T8872 18329-18337 NN denotes sequence
T8873 18342-18343 -LRB- denotes (
T8874 18343-18346 NN denotes EST
T8875 18346-18347 -RRB- denotes )
T8876 18357-18359 , denotes ,
T8877 18359-18361 PRP denotes we
T8878 18371-18378 NNS denotes primers
T8879 18379-18381 TO denotes to
T8880 18389-18396 VBP denotes amplify
T8881 18382-18384 NN denotes RT
T8882 18384-18385 HYPH denotes -
T8883 18385-18388 NN denotes PCR
T8884 18397-18398 DT denotes a
T8885 18412-18416 NN denotes cDNA
T8886 18399-18403 NN denotes TIF1
T8887 18403-18404 HYPH denotes -
T8888 18404-18411 VBN denotes related
T8889 18417-18421 IN denotes from
T8890 18422-18431 JJ denotes embryonic
T8891 18450-18453 NN denotes RNA
T8892 18432-18434 CD denotes 10
T8893 18435-18438 NN denotes hpf
T8894 18434-18435 HYPH denotes -
T8895 18439-18442 CC denotes and
T8896 18443-18445 CD denotes 24
T8897 18446-18449 NN denotes hpf
T8898 18445-18446 HYPH denotes -
T8899 18453-18454 . denotes .
T8900 18454-18575 sentence denotes This cDNA encodes a predicted zebrafish ortholog of human TIF1α based on predicted amino acid sequences (see Figure 3B).
T8901 18455-18459 DT denotes This
T8902 18460-18464 NN denotes cDNA
T8903 18465-18472 VBZ denotes encodes
T8904 18473-18474 DT denotes a
T8905 18495-18503 NN denotes ortholog
T8906 18475-18484 JJ denotes predicted
T8907 18485-18494 NN denotes zebrafish
T8908 18504-18506 IN denotes of
T8909 18507-18512 JJ denotes human
T8910 18513-18518 NN denotes TIF1α
T8911 18519-18524 VBN denotes based
T8912 18525-18527 IN denotes on
T8913 18528-18537 JJ denotes predicted
T8914 18549-18558 NNS denotes sequences
T8915 18538-18543 NN denotes amino
T8916 18544-18548 NN denotes acid
T8917 18559-18560 -LRB- denotes (
T8918 18560-18563 VB denotes see
T8919 18564-18570 NN denotes Figure
T8920 18571-18573 CD denotes 3B
T8921 18573-18574 -RRB- denotes )
T8922 18574-18575 . denotes .
T8923 18575-18883 sentence denotes In addition, zebrafish tif1α ESTs map to LG4 in a region predicted to be syntenic to the region of human Chromosome 7 that contains the TIF1α gene based on the conserved locations of eight other orthologous gene pairs, including SEMA3A, mapped to these regions in human and zebrafish (Barbazuk et al. 2000).
T8924 18576-18578 IN denotes In
T8925 18610-18613 VBP denotes map
T8926 18579-18587 NN denotes addition
T8927 18587-18589 , denotes ,
T8928 18589-18598 NN denotes zebrafish
T8929 18605-18609 NNS denotes ESTs
T8930 18599-18604 NN denotes tif1α
T8931 18614-18616 IN denotes to
T8932 18617-18620 NN denotes LG4
T8933 18621-18623 IN denotes in
T8934 18624-18625 DT denotes a
T8935 18626-18632 NN denotes region
T8936 18633-18642 VBN denotes predicted
T8937 18643-18645 TO denotes to
T8938 18646-18648 VB denotes be
T8939 18649-18657 JJ denotes syntenic
T8940 18658-18660 IN denotes to
T8941 18661-18664 DT denotes the
T8942 18665-18671 NN denotes region
T8943 18672-18674 IN denotes of
T8944 18675-18680 JJ denotes human
T8945 18681-18691 NN denotes Chromosome
T8946 18692-18693 CD denotes 7
T8947 18694-18698 WDT denotes that
T8948 18699-18707 VBZ denotes contains
T8949 18708-18711 DT denotes the
T8950 18718-18722 NN denotes gene
T8951 18712-18717 NN denotes TIF1α
T8952 18723-18728 VBN denotes based
T8953 18729-18731 IN denotes on
T8954 18732-18735 DT denotes the
T8955 18746-18755 NNS denotes locations
T8956 18736-18745 JJ denotes conserved
T8957 18756-18758 IN denotes of
T8958 18759-18764 CD denotes eight
T8959 18788-18793 NNS denotes pairs
T8960 18765-18770 JJ denotes other
T8961 18771-18782 JJ denotes orthologous
T8962 18783-18787 NN denotes gene
T8963 18793-18795 , denotes ,
T8964 18795-18804 VBG denotes including
T8965 18805-18811 NN denotes SEMA3A
T8966 18811-18813 , denotes ,
T8967 18813-18819 VBN denotes mapped
T8968 18820-18822 IN denotes to
T8969 18823-18828 DT denotes these
T8970 18829-18836 NNS denotes regions
T8971 18837-18839 IN denotes in
T8972 18840-18845 JJ denotes human
T8973 18850-18859 NN denotes zebrafish
T8974 18846-18849 CC denotes and
T8975 18860-18861 -LRB- denotes (
T8976 18861-18869 NNP denotes Barbazuk
T8977 18870-18872 FW denotes et
T8978 18873-18876 FW denotes al.
T8979 18877-18881 CD denotes 2000
T8980 18881-18882 -RRB- denotes )
T8981 18882-18883 . denotes .
T8982 18883-18982 sentence denotes We next compared the embryonic expression pattern of tif1α mRNA to tif1γ by in situ hybridization.
T8983 18884-18886 PRP denotes We
T8984 18892-18900 VBD denotes compared
T8985 18887-18891 RB denotes next
T8986 18901-18904 DT denotes the
T8987 18926-18933 NN denotes pattern
T8988 18905-18914 JJ denotes embryonic
T8989 18915-18925 NN denotes expression
T8990 18934-18936 IN denotes of
T8991 18937-18942 NN denotes tif1α
T8992 18943-18947 NN denotes mRNA
T8993 18948-18950 IN denotes to
T8994 18951-18956 NN denotes tif1γ
T8995 18957-18959 IN denotes by
T8996 18960-18962 FW denotes in
T8997 18963-18967 FW denotes situ
T8998 18968-18981 NN denotes hybridization
T8999 18981-18982 . denotes .
T9000 18982-19129 sentence denotes Like mammalian TIF1α (Le Douarin et al. 1995; Niederreither et al. 1999), the predicted zebrafish tif1γ gene is broadly expressed (see Figure 4A).
T9001 18983-18987 IN denotes Like
T9002 19103-19112 VBN denotes expressed
T9003 18988-18997 JJ denotes mammalian
T9004 18998-19003 NN denotes TIF1α
T9005 19004-19005 -LRB- denotes (
T9006 19005-19007 NNP denotes Le
T9007 19008-19015 NNP denotes Douarin
T9008 19016-19018 FW denotes et
T9009 19019-19022 FW denotes al.
T9010 19023-19027 CD denotes 1995
T9011 19027-19028 : denotes ;
T9012 19029-19042 NNP denotes Niederreither
T9013 19043-19045 FW denotes et
T9014 19046-19049 FW denotes al.
T9015 19050-19054 CD denotes 1999
T9016 19054-19055 -RRB- denotes )
T9017 19055-19057 , denotes ,
T9018 19057-19060 DT denotes the
T9019 19087-19091 NN denotes gene
T9020 19061-19070 JJ denotes predicted
T9021 19071-19080 NN denotes zebrafish
T9022 19081-19086 NN denotes tif1γ
T9023 19092-19094 VBZ denotes is
T9024 19095-19102 RB denotes broadly
T9025 19113-19114 -LRB- denotes (
T9026 19114-19117 VB denotes see
T9027 19118-19124 NN denotes Figure
T9028 19125-19127 CD denotes 4A
T9029 19127-19128 -RRB- denotes )
T9030 19128-19129 . denotes .
T9031 19129-19284 sentence denotes At five somites, zebrafish tif1α does not display the relatively high expression in the horseshoe-shaped region of hematopoietic mesoderm seen with tif1γ.
T9032 19130-19132 IN denotes At
T9033 19172-19179 VB denotes display
T9034 19133-19137 CD denotes five
T9035 19138-19145 NNS denotes somites
T9036 19145-19147 , denotes ,
T9037 19147-19156 NN denotes zebrafish
T9038 19157-19162 NN denotes tif1α
T9039 19163-19167 VBZ denotes does
T9040 19168-19171 RB denotes not
T9041 19180-19183 DT denotes the
T9042 19200-19210 NN denotes expression
T9043 19184-19194 RB denotes relatively
T9044 19195-19199 JJ denotes high
T9045 19211-19213 IN denotes in
T9046 19214-19217 DT denotes the
T9047 19235-19241 NN denotes region
T9048 19218-19227 NN denotes horseshoe
T9049 19227-19228 HYPH denotes -
T9050 19228-19234 VBN denotes shaped
T9051 19242-19244 IN denotes of
T9052 19245-19258 JJ denotes hematopoietic
T9053 19259-19267 NN denotes mesoderm
T9054 19268-19272 VBN denotes seen
T9055 19273-19277 IN denotes with
T9056 19278-19283 NN denotes tif1γ
T9057 19283-19284 . denotes .
T9058 19284-19398 sentence denotes At later stages, tif1α is evenly expressed throughout most of the embryo, including the developing blood islands.
T9059 19285-19287 IN denotes At
T9060 19318-19327 VBN denotes expressed
T9061 19288-19293 JJ denotes later
T9062 19294-19300 NNS denotes stages
T9063 19300-19302 , denotes ,
T9064 19302-19307 NN denotes tif1α
T9065 19308-19310 VBZ denotes is
T9066 19311-19317 RB denotes evenly
T9067 19328-19338 IN denotes throughout
T9068 19339-19343 JJS denotes most
T9069 19344-19346 IN denotes of
T9070 19347-19350 DT denotes the
T9071 19351-19357 NN denotes embryo
T9072 19357-19359 , denotes ,
T9073 19359-19368 VBG denotes including
T9074 19369-19372 DT denotes the
T9075 19390-19397 NNS denotes islands
T9076 19373-19383 VBG denotes developing
T9077 19384-19389 NN denotes blood
T9078 19397-19398 . denotes .
T9079 19398-19524 sentence denotes Therefore, tif1α is coexpressed in the same cells with tif1γ and may therefore be available to form hetero-oligomers in vivo.
T9080 19399-19408 RB denotes Therefore
T9081 19419-19430 VBN denotes coexpressed
T9082 19408-19410 , denotes ,
T9083 19410-19415 NN denotes tif1α
T9084 19416-19418 VBZ denotes is
T9085 19431-19433 IN denotes in
T9086 19434-19437 DT denotes the
T9087 19443-19448 NNS denotes cells
T9088 19438-19442 JJ denotes same
T9089 19449-19453 IN denotes with
T9090 19454-19459 NN denotes tif1γ
T9091 19460-19463 CC denotes and
T9092 19464-19467 MD denotes may
T9093 19478-19480 VB denotes be
T9094 19468-19477 RB denotes therefore
T9095 19481-19490 JJ denotes available
T9096 19491-19493 TO denotes to
T9097 19494-19498 VB denotes form
T9098 19499-19515 NNS denotes hetero-oligomers
T9099 19516-19518 FW denotes in
T9100 19519-19523 FW denotes vivo
T9101 19523-19524 . denotes .
T9696 19976-20220 sentence denotes At 4 d of development, 70% (n = 10) of montg234 mutants show significant (greater than 200 cells in comparison to a wild-type estimate of 3,000 cells) rescue of circulating hemoglobinized RBCs in comparison to control sibling mutants (n = 75).
T9620 19526-19532 VBN denotes Forced
T9621 19533-19543 NN denotes Expression
T9622 19544-19546 IN denotes of
T9623 19547-19552 NN denotes tif1γ
T9624 19561-19574 NN denotes Hematopoiesis
T9625 19553-19560 VBZ denotes Rescues
T9626 19575-19577 IN denotes in
T9627 19578-19581 NN denotes mon
T9628 19582-19589 NNS denotes Mutants
T9629 19589-19757 sentence denotes To further confirm that a mutation in the zebrafish tif1γ gene is responsible for the mon mutant phenotype we performed embryo rescue experiments (Figure 5A; Table 1).
T9630 19590-19592 TO denotes To
T9631 19601-19608 VB denotes confirm
T9632 19593-19600 RB denotes further
T9633 19700-19709 VBD denotes performed
T9634 19609-19613 IN denotes that
T9635 19653-19655 VBZ denotes is
T9636 19614-19615 DT denotes a
T9637 19616-19624 NN denotes mutation
T9638 19625-19627 IN denotes in
T9639 19628-19631 DT denotes the
T9640 19648-19652 NN denotes gene
T9641 19632-19641 NN denotes zebrafish
T9642 19642-19647 NN denotes tif1γ
T9643 19656-19667 JJ denotes responsible
T9644 19668-19671 IN denotes for
T9645 19672-19675 DT denotes the
T9646 19687-19696 NN denotes phenotype
T9647 19676-19679 NN denotes mon
T9648 19680-19686 JJ denotes mutant
T9649 19697-19699 PRP denotes we
T9650 19710-19716 NN denotes embryo
T9651 19724-19735 NNS denotes experiments
T9652 19717-19723 NN denotes rescue
T9653 19736-19737 -LRB- denotes (
T9654 19748-19753 NN denotes Table
T9655 19737-19743 NN denotes Figure
T9656 19744-19746 CD denotes 5A
T9657 19746-19747 : denotes ;
T9658 19754-19755 CD denotes 1
T9659 19755-19756 -RRB- denotes )
T9660 19756-19757 . denotes .
T9661 19757-19976 sentence denotes Microinjection of synthetic wild-type mon mRNA at the one-cell stage rescues the formation of embryonic erythrocytes in genotyped mutant embryos without causing obvious defects in embryonic patterning or organogenesis.
T9662 19758-19772 NN denotes Microinjection
T9663 19827-19834 VBZ denotes rescues
T9664 19773-19775 IN denotes of
T9665 19776-19785 JJ denotes synthetic
T9666 19800-19804 NN denotes mRNA
T9667 19786-19790 JJ denotes wild
T9668 19791-19795 NN denotes type
T9669 19790-19791 HYPH denotes -
T9670 19796-19799 NN denotes mon
T9671 19805-19807 IN denotes at
T9672 19808-19811 DT denotes the
T9673 19821-19826 NN denotes stage
T9674 19812-19815 CD denotes one
T9675 19816-19820 NN denotes cell
T9676 19815-19816 HYPH denotes -
T9677 19835-19838 DT denotes the
T9678 19839-19848 NN denotes formation
T9679 19849-19851 IN denotes of
T9680 19852-19861 JJ denotes embryonic
T9681 19862-19874 NNS denotes erythrocytes
T9682 19875-19877 IN denotes in
T9683 19878-19887 VBN denotes genotyped
T9684 19895-19902 NNS denotes embryos
T9685 19888-19894 JJ denotes mutant
T9686 19903-19910 IN denotes without
T9687 19911-19918 VBG denotes causing
T9688 19919-19926 JJ denotes obvious
T9689 19927-19934 NNS denotes defects
T9690 19935-19937 IN denotes in
T9691 19938-19947 JJ denotes embryonic
T9692 19948-19958 NN denotes patterning
T9693 19959-19961 CC denotes or
T9694 19962-19975 NN denotes organogenesis
T9695 19975-19976 . denotes .
T9697 19977-19979 IN denotes At
T9698 20033-20037 VBP denotes show
T9699 19980-19981 CD denotes 4
T9700 19982-19983 NN denotes d
T9701 19984-19986 IN denotes of
T9702 19987-19998 NN denotes development
T9703 19998-20000 , denotes ,
T9704 20000-20002 CD denotes 70
T9705 20002-20003 NN denotes %
T9706 20004-20005 -LRB- denotes (
T9707 20009-20011 CD denotes 10
T9708 20005-20006 NN denotes n
T9709 20007-20008 SYM denotes =
T9710 20011-20012 -RRB- denotes )
T9711 20013-20015 IN denotes of
T9712 20016-20024 NN denotes montg234
T9713 20025-20032 NNS denotes mutants
T9714 20038-20049 JJ denotes significant
T9715 20128-20134 NN denotes rescue
T9716 20050-20051 -LRB- denotes (
T9717 20068-20073 NNS denotes cells
T9718 20051-20058 JJR denotes greater
T9719 20064-20067 CD denotes 200
T9720 20059-20063 IN denotes than
T9721 20074-20076 IN denotes in
T9722 20077-20087 NN denotes comparison
T9723 20088-20090 IN denotes to
T9724 20091-20092 DT denotes a
T9725 20103-20111 NN denotes estimate
T9726 20093-20097 JJ denotes wild
T9727 20098-20102 NN denotes type
T9728 20097-20098 HYPH denotes -
T9729 20112-20114 IN denotes of
T9730 20115-20120 CD denotes 3,000
T9731 20121-20126 NNS denotes cells
T9732 20126-20127 -RRB- denotes )
T9733 20135-20137 IN denotes of
T9734 20138-20149 VBG denotes circulating
T9735 20165-20169 NNS denotes RBCs
T9736 20150-20164 VBN denotes hemoglobinized
T9737 20170-20172 IN denotes in
T9738 20173-20183 NN denotes comparison
T9739 20184-20186 IN denotes to
T9740 20187-20194 NN denotes control
T9741 20203-20210 NNS denotes mutants
T9742 20195-20202 NN denotes sibling
T9743 20211-20212 -LRB- denotes (
T9744 20216-20218 CD denotes 75
T9745 20212-20213 NN denotes n
T9746 20214-20215 SYM denotes =
T9747 20218-20219 -RRB- denotes )
T9748 20219-20220 . denotes .
T9749 20220-20387 sentence denotes Based on the correction of the jagged fin-fold phenotype (Ransom et al. 1996), the mesenchymal cells are rescued to a similar extent as the anemia (unpublished data).
T9750 20221-20226 VBN denotes Based
T9751 20326-20333 VBN denotes rescued
T9752 20227-20229 IN denotes on
T9753 20230-20233 DT denotes the
T9754 20234-20244 NN denotes correction
T9755 20245-20247 IN denotes of
T9756 20248-20251 DT denotes the
T9757 20268-20277 NN denotes phenotype
T9758 20252-20258 JJ denotes jagged
T9759 20259-20262 NN denotes fin
T9760 20263-20267 NN denotes fold
T9761 20262-20263 HYPH denotes -
T9762 20278-20279 -LRB- denotes (
T9763 20279-20285 NNP denotes Ransom
T9764 20286-20288 FW denotes et
T9765 20289-20292 FW denotes al.
T9766 20293-20297 CD denotes 1996
T9767 20297-20298 -RRB- denotes )
T9768 20298-20300 , denotes ,
T9769 20300-20303 DT denotes the
T9770 20316-20321 NNS denotes cells
T9771 20304-20315 JJ denotes mesenchymal
T9772 20322-20325 VBP denotes are
T9773 20334-20336 IN denotes to
T9774 20337-20338 DT denotes a
T9775 20347-20353 NN denotes extent
T9776 20339-20346 JJ denotes similar
T9777 20354-20356 IN denotes as
T9778 20357-20360 DT denotes the
T9779 20361-20367 NN denotes anemia
T9780 20368-20369 -LRB- denotes (
T9781 20381-20385 NNS denotes data
T9782 20369-20380 JJ denotes unpublished
T9783 20385-20386 -RRB- denotes )
T9784 20386-20387 . denotes .
T9785 20387-20564 sentence denotes Overexpression of mon did not result in expanded blood cell numbers in wild-type embryos and was not toxic at doses that rescue the phenotype of mon mutants (unpublished data).
T9786 20388-20402 NN denotes Overexpression
T9787 20418-20424 VB denotes result
T9788 20403-20405 IN denotes of
T9789 20406-20409 NN denotes mon
T9790 20410-20413 VBD denotes did
T9791 20414-20417 RB denotes not
T9792 20425-20427 IN denotes in
T9793 20428-20436 JJ denotes expanded
T9794 20448-20455 NNS denotes numbers
T9795 20437-20442 NN denotes blood
T9796 20443-20447 NN denotes cell
T9797 20456-20458 IN denotes in
T9798 20459-20463 JJ denotes wild
T9799 20464-20468 NN denotes type
T9800 20463-20464 HYPH denotes -
T9801 20469-20476 NNS denotes embryos
T9802 20477-20480 CC denotes and
T9803 20481-20484 VBD denotes was
T9804 20485-20488 RB denotes not
T9805 20489-20494 JJ denotes toxic
T9806 20495-20497 IN denotes at
T9807 20498-20503 NNS denotes doses
T9808 20504-20508 WDT denotes that
T9809 20509-20515 VBP denotes rescue
T9810 20516-20519 DT denotes the
T9811 20520-20529 NN denotes phenotype
T9812 20530-20532 IN denotes of
T9813 20533-20536 NN denotes mon
T9814 20537-20544 NNS denotes mutants
T9815 20545-20546 -LRB- denotes (
T9816 20558-20562 NNS denotes data
T9817 20546-20557 JJ denotes unpublished
T9818 20562-20563 -RRB- denotes )
T9819 20563-20564 . denotes .
T9820 20564-20741 sentence denotes Since there were no expanded or ectopic blood populations in the embryos, these rescue experiments suggest that mon functions as a permissive factor required for hematopoiesis.
T9821 20565-20570 IN denotes Since
T9822 20577-20581 VBD denotes were
T9823 20571-20576 EX denotes there
T9824 20664-20671 VBP denotes suggest
T9825 20582-20584 DT denotes no
T9826 20611-20622 NNS denotes populations
T9827 20585-20593 JJ denotes expanded
T9828 20594-20596 CC denotes or
T9829 20597-20604 JJ denotes ectopic
T9830 20605-20610 NN denotes blood
T9831 20623-20625 IN denotes in
T9832 20626-20629 DT denotes the
T9833 20630-20637 NNS denotes embryos
T9834 20637-20639 , denotes ,
T9835 20639-20644 DT denotes these
T9836 20652-20663 NNS denotes experiments
T9837 20645-20651 NN denotes rescue
T9838 20672-20676 IN denotes that
T9839 20681-20690 VBZ denotes functions
T9840 20677-20680 NN denotes mon
T9841 20691-20693 IN denotes as
T9842 20694-20695 DT denotes a
T9843 20707-20713 NN denotes factor
T9844 20696-20706 JJ denotes permissive
T9845 20714-20722 VBN denotes required
T9846 20723-20726 IN denotes for
T9847 20727-20740 NN denotes hematopoiesis
T9848 20740-20741 . denotes .
T10666 22442-22448 NN denotes Marrow
T10667 22449-22464 NN denotes Transplantation
T10668 22465-22472 VBZ denotes Rescues
T10669 22473-22487 NN denotes Erythropoiesis
T10670 22488-22490 IN denotes in
T10671 22491-22494 NN denotes mon
T10672 22495-22502 NNS denotes Mutants
T10673 22502-22657 sentence denotes The high levels of tif1γ expression in erythroid cells suggest that it functions as a cell-autonomous regulator of gene expression in hematopoietic cells.
T10674 22503-22506 DT denotes The
T10675 22512-22518 NNS denotes levels
T10676 22507-22511 JJ denotes high
T10677 22558-22565 VBP denotes suggest
T10678 22519-22521 IN denotes of
T10679 22522-22527 NN denotes tif1γ
T10680 22528-22538 NN denotes expression
T10681 22539-22541 IN denotes in
T10682 22542-22551 JJ denotes erythroid
T10683 22552-22557 NNS denotes cells
T10684 22566-22570 IN denotes that
T10685 22574-22583 VBZ denotes functions
T10686 22571-22573 PRP denotes it
T10687 22584-22586 IN denotes as
T10688 22587-22588 DT denotes a
T10689 22605-22614 NN denotes regulator
T10690 22589-22593 NN denotes cell
T10691 22594-22604 JJ denotes autonomous
T10692 22593-22594 HYPH denotes -
T10693 22615-22617 IN denotes of
T10694 22618-22622 NN denotes gene
T10695 22623-22633 NN denotes expression
T10696 22634-22636 IN denotes in
T10697 22637-22650 JJ denotes hematopoietic
T10698 22651-22656 NNS denotes cells
T10699 22656-22657 . denotes .
T10700 22657-22865 sentence denotes In order to test this hypothesis, we transplanted wild-type adult zebrafish kidney marrow cells carrying a gata1:green fluorescent protein (GFP) transgene into 48-hpf mon mutant embryos (Figure 5B; Table 2).
T10701 22658-22660 IN denotes In
T10702 22695-22707 VBD denotes transplanted
T10703 22661-22666 NN denotes order
T10704 22667-22669 TO denotes to
T10705 22670-22674 VB denotes test
T10706 22675-22679 DT denotes this
T10707 22680-22690 NN denotes hypothesis
T10708 22690-22692 , denotes ,
T10709 22692-22694 PRP denotes we
T10710 22708-22712 JJ denotes wild
T10711 22713-22717 NN denotes type
T10712 22712-22713 HYPH denotes -
T10713 22748-22753 NNS denotes cells
T10714 22718-22723 JJ denotes adult
T10715 22724-22733 NN denotes zebrafish
T10716 22734-22740 NN denotes kidney
T10717 22741-22747 NN denotes marrow
T10718 22754-22762 VBG denotes carrying
T10719 22763-22764 DT denotes a
T10720 22803-22812 NN denotes transgene
T10721 22765-22770 NN denotes gata1
T10722 22770-22771 : denotes :
T10723 22771-22776 JJ denotes green
T10724 22789-22796 NN denotes protein
T10725 22777-22788 JJ denotes fluorescent
T10726 22797-22798 -LRB- denotes (
T10727 22798-22801 NN denotes GFP
T10728 22801-22802 -RRB- denotes )
T10729 22813-22817 IN denotes into
T10730 22818-22820 CD denotes 48
T10731 22821-22824 NN denotes hpf
T10732 22820-22821 HYPH denotes -
T10733 22836-22843 NNS denotes embryos
T10734 22825-22828 NN denotes mon
T10735 22829-22835 JJ denotes mutant
T10736 22844-22845 -LRB- denotes (
T10737 22856-22861 NN denotes Table
T10738 22845-22851 NN denotes Figure
T10739 22852-22854 CD denotes 5B
T10740 22854-22855 : denotes ;
T10741 22862-22863 CD denotes 2
T10742 22863-22864 -RRB- denotes )
T10743 22864-22865 . denotes .
T10744 22865-23024 sentence denotes The gata1:GFP transgene makes use of the gata1 promoter to drive GFP expression and can thus be used to mark donor-derived erythroid cells (Long et al. 1997).
T10745 22866-22869 DT denotes The
T10746 22880-22889 NN denotes transgene
T10747 22870-22875 NN denotes gata1
T10748 22876-22879 NN denotes GFP
T10749 22875-22876 : denotes :
T10750 22890-22895 VBZ denotes makes
T10751 22896-22899 NN denotes use
T10752 22900-22902 IN denotes of
T10753 22903-22906 DT denotes the
T10754 22913-22921 NN denotes promoter
T10755 22907-22912 NN denotes gata1
T10756 22922-22924 TO denotes to
T10757 22925-22930 VB denotes drive
T10758 22931-22934 NN denotes GFP
T10759 22935-22945 NN denotes expression
T10760 22946-22949 CC denotes and
T10761 22950-22953 MD denotes can
T10762 22962-22966 VBN denotes used
T10763 22954-22958 RB denotes thus
T10764 22959-22961 VB denotes be
T10765 22967-22969 TO denotes to
T10766 22970-22974 VB denotes mark
T10767 22975-22980 NN denotes donor
T10768 22981-22988 VBN denotes derived
T10769 22980-22981 HYPH denotes -
T10770 22999-23004 NNS denotes cells
T10771 22989-22998 JJ denotes erythroid
T10772 23005-23006 -LRB- denotes (
T10773 23006-23010 NNP denotes Long
T10774 23011-23013 FW denotes et
T10775 23014-23017 FW denotes al.
T10776 23018-23022 CD denotes 1997
T10777 23022-23023 -RRB- denotes )
T10778 23023-23024 . denotes .
T10779 23024-23100 sentence denotes Untransplanted mutant embryos have no embryonic blood cells in circulation.
T10780 23025-23039 JJ denotes Untransplanted
T10781 23047-23054 NNS denotes embryos
T10782 23040-23046 JJ denotes mutant
T10783 23055-23059 VBP denotes have
T10784 23060-23062 DT denotes no
T10785 23079-23084 NNS denotes cells
T10786 23063-23072 JJ denotes embryonic
T10787 23073-23078 NN denotes blood
T10788 23085-23087 IN denotes in
T10789 23088-23099 NN denotes circulation
T10790 23099-23100 . denotes .
T10791 23100-23177 sentence denotes Following transplantation, mutant host embryos were observed daily for 2 wk.
T10792 23101-23110 VBG denotes Following
T10793 23153-23161 VBN denotes observed
T10794 23111-23126 NN denotes transplantation
T10795 23126-23128 , denotes ,
T10796 23128-23134 JJ denotes mutant
T10797 23140-23147 NNS denotes embryos
T10798 23135-23139 NN denotes host
T10799 23148-23152 VBD denotes were
T10800 23162-23167 RB denotes daily
T10801 23168-23171 IN denotes for
T10802 23172-23173 CD denotes 2
T10803 23174-23176 NN denotes wk
T10804 23176-23177 . denotes .
T10805 23177-23263 sentence denotes Of 191 mutant embryos injected, 129 (68%) showed GFP+ cells in circulation 2 d later.
T10806 23178-23180 IN denotes Of
T10807 23220-23226 VBD denotes showed
T10808 23181-23184 CD denotes 191
T10809 23192-23199 NNS denotes embryos
T10810 23185-23191 JJ denotes mutant
T10811 23200-23208 VBN denotes injected
T10812 23208-23210 , denotes ,
T10813 23210-23213 CD denotes 129
T10814 23214-23215 -LRB- denotes (
T10815 23217-23218 NN denotes %
T10816 23215-23217 CD denotes 68
T10817 23218-23219 -RRB- denotes )
T10818 23227-23230 NN denotes GFP
T10819 23232-23237 NNS denotes cells
T10820 23230-23231 SYM denotes +
T10821 23238-23240 IN denotes in
T10822 23241-23252 NN denotes circulation
T10823 23253-23254 CD denotes 2
T10824 23255-23256 NN denotes d
T10825 23257-23262 RB denotes later
T10826 23262-23263 . denotes .
T10827 23263-23355 sentence denotes Many recipients showed robust increases in donor-derived cells over the observation period.
T10828 23264-23268 JJ denotes Many
T10829 23269-23279 NNS denotes recipients
T10830 23280-23286 VBD denotes showed
T10831 23287-23293 JJ denotes robust
T10832 23294-23303 NNS denotes increases
T10833 23304-23306 IN denotes in
T10834 23307-23312 NN denotes donor
T10835 23321-23326 NNS denotes cells
T10836 23312-23313 HYPH denotes -
T10837 23313-23320 VBN denotes derived
T10838 23327-23331 IN denotes over
T10839 23332-23335 DT denotes the
T10840 23348-23354 NN denotes period
T10841 23336-23347 NN denotes observation
T10842 23354-23355 . denotes .
T10843 23355-23569 sentence denotes Of 81 recipients initially scored as having less than ten GFP+ cells at day 2 posttransplant, 13 (16%) of these demonstrated a marked increase in erythroid cells with 100–1,000 GFP+ cells in circulation 6 d later.
T10844 23356-23358 IN denotes Of
T10845 23468-23480 VBD denotes demonstrated
T10846 23359-23361 CD denotes 81
T10847 23362-23372 NNS denotes recipients
T10848 23373-23382 RB denotes initially
T10849 23383-23389 VBN denotes scored
T10850 23390-23392 IN denotes as
T10851 23393-23399 VBG denotes having
T10852 23400-23404 JJR denotes less
T10853 23410-23413 CD denotes ten
T10854 23405-23409 IN denotes than
T10855 23419-23424 NNS denotes cells
T10856 23414-23417 NN denotes GFP
T10857 23417-23418 SYM denotes +
T10858 23425-23427 IN denotes at
T10859 23428-23431 NN denotes day
T10860 23432-23433 CD denotes 2
T10861 23434-23448 RB denotes posttransplant
T10862 23448-23450 , denotes ,
T10863 23450-23452 CD denotes 13
T10864 23453-23454 -LRB- denotes (
T10865 23456-23457 NN denotes %
T10866 23454-23456 CD denotes 16
T10867 23457-23458 -RRB- denotes )
T10868 23459-23461 IN denotes of
T10869 23462-23467 DT denotes these
T10870 23481-23482 DT denotes a
T10871 23490-23498 NN denotes increase
T10872 23483-23489 JJ denotes marked
T10873 23499-23501 IN denotes in
T10874 23502-23511 JJ denotes erythroid
T10875 23512-23517 NNS denotes cells
T10876 23518-23522 IN denotes with
T10877 23544-23546 IN denotes in
T10878 23523-23526 CD denotes 100
T10879 23527-23532 CD denotes 1,000
T10880 23526-23527 SYM denotes –
T10881 23538-23543 NNS denotes cells
T10882 23533-23537 NN denotes GFP+
T10883 23547-23558 NN denotes circulation
T10884 23559-23560 CD denotes 6
T10885 23561-23562 NNS denotes d
T10886 23563-23568 RB denotes later
T10887 23568-23569 . denotes .
T10888 23569-23712 sentence denotes By day 10, these transplanted embryos showed approximately 3,000 cells in circulation, similar to the number of blood cells in normal embryos.
T10889 23570-23572 IN denotes By
T10890 23608-23614 VBD denotes showed
T10891 23573-23576 NN denotes day
T10892 23577-23579 CD denotes 10
T10893 23579-23581 , denotes ,
T10894 23581-23586 DT denotes these
T10895 23600-23607 NNS denotes embryos
T10896 23587-23599 JJ denotes transplanted
T10897 23615-23628 RB denotes approximately
T10898 23629-23634 CD denotes 3,000
T10899 23635-23640 NNS denotes cells
T10900 23641-23643 IN denotes in
T10901 23644-23655 NN denotes circulation
T10902 23655-23657 , denotes ,
T10903 23657-23664 JJ denotes similar
T10904 23665-23667 IN denotes to
T10905 23668-23671 DT denotes the
T10906 23672-23678 NN denotes number
T10907 23679-23681 IN denotes of
T10908 23682-23687 NN denotes blood
T10909 23688-23693 NNS denotes cells
T10910 23694-23696 IN denotes in
T10911 23697-23703 JJ denotes normal
T10912 23704-23711 NNS denotes embryos
T10913 23711-23712 . denotes .
T10914 23712-23911 sentence denotes Despite robust reconstitution of blood cells, mutant recipients did not inflate their swim bladders and thus failed to survive longer than nontransplanted sibling controls, all dying by 3 wk of age.
T10915 23713-23720 IN denotes Despite
T10916 23785-23792 VB denotes inflate
T10917 23721-23727 JJ denotes robust
T10918 23728-23742 NN denotes reconstitution
T10919 23743-23745 IN denotes of
T10920 23746-23751 NN denotes blood
T10921 23752-23757 NNS denotes cells
T10922 23757-23759 , denotes ,
T10923 23759-23765 JJ denotes mutant
T10924 23766-23776 NNS denotes recipients
T10925 23777-23780 VBD denotes did
T10926 23781-23784 RB denotes not
T10927 23793-23798 PRP$ denotes their
T10928 23804-23812 NNS denotes bladders
T10929 23799-23803 NN denotes swim
T10930 23813-23816 CC denotes and
T10931 23817-23821 RB denotes thus
T10932 23822-23828 VBD denotes failed
T10933 23829-23831 TO denotes to
T10934 23832-23839 VB denotes survive
T10935 23840-23846 RBR denotes longer
T10936 23847-23851 IN denotes than
T10937 23852-23867 JJ denotes nontransplanted
T10938 23876-23884 NNS denotes controls
T10939 23868-23875 NN denotes sibling
T10940 23884-23886 , denotes ,
T10941 23886-23889 DT denotes all
T10942 23890-23895 VBG denotes dying
T10943 23896-23898 IN denotes by
T10944 23899-23900 CD denotes 3
T10945 23901-23903 NN denotes wk
T10946 23904-23906 IN denotes of
T10947 23907-23910 NN denotes age
T10948 23910-23911 . denotes .
T10949 23911-23999 sentence denotes In contrast, 13/35 (37%) heterozygous montg234 transplants survived to early adulthood.
T10950 23912-23914 IN denotes In
T10951 23971-23979 VBD denotes survived
T10952 23915-23923 NN denotes contrast
T10953 23923-23925 , denotes ,
T10954 23925-23927 CD denotes 13
T10955 23928-23930 CD denotes 35
T10956 23927-23928 SYM denotes /
T10957 23959-23970 NNS denotes transplants
T10958 23931-23932 -LRB- denotes (
T10959 23934-23935 NN denotes %
T10960 23932-23934 CD denotes 37
T10961 23935-23936 -RRB- denotes )
T10962 23937-23949 JJ denotes heterozygous
T10963 23950-23958 NN denotes montg234
T10964 23980-23982 IN denotes to
T10965 23983-23988 JJ denotes early
T10966 23989-23998 NN denotes adulthood
T10967 23998-23999 . denotes .
T10968 23999-24104 sentence denotes Similar transplants of wild-type cells can fully rescue vlad tepes (gata1) mutants (Traver et al. 2003).
T10969 24000-24007 JJ denotes Similar
T10970 24008-24019 NNS denotes transplants
T10971 24049-24055 VB denotes rescue
T10972 24020-24022 IN denotes of
T10973 24023-24027 JJ denotes wild
T10974 24028-24032 NN denotes type
T10975 24027-24028 HYPH denotes -
T10976 24033-24038 NNS denotes cells
T10977 24039-24042 MD denotes can
T10978 24043-24048 RB denotes fully
T10979 24056-24060 NN denotes vlad
T10980 24061-24066 NN denotes tepes
T10981 24075-24082 NNS denotes mutants
T10982 24067-24068 -LRB- denotes (
T10983 24068-24073 NN denotes gata1
T10984 24073-24074 -RRB- denotes )
T10985 24083-24084 -LRB- denotes (
T10986 24084-24090 NNP denotes Traver
T10987 24091-24093 FW denotes et
T10988 24094-24097 FW denotes al.
T10989 24098-24102 CD denotes 2003
T10990 24102-24103 -RRB- denotes )
T10991 24103-24104 . denotes .
T10992 24104-24349 sentence denotes Therefore, the results of cell transplantations suggests that tif1γ plays a cell-autonomous role in erythroid cells, and its role in nonhematopoietic tissues, such as trunk mesenchyme or the nervous system, is also required for embryo survival.
T10993 24105-24114 RB denotes Therefore
T10994 24153-24161 VBZ denotes suggests
T10995 24114-24116 , denotes ,
T10996 24116-24119 DT denotes the
T10997 24120-24127 NNS denotes results
T10998 24128-24130 IN denotes of
T10999 24131-24135 NN denotes cell
T11000 24136-24152 NNS denotes transplantations
T11001 24162-24166 IN denotes that
T11002 24173-24178 VBZ denotes plays
T11003 24167-24172 NN denotes tif1γ
T11004 24179-24180 DT denotes a
T11005 24197-24201 NN denotes role
T11006 24181-24185 NN denotes cell
T11007 24186-24196 JJ denotes autonomous
T11008 24185-24186 HYPH denotes -
T11009 24202-24204 IN denotes in
T11010 24205-24214 JJ denotes erythroid
T11011 24215-24220 NNS denotes cells
T11012 24220-24222 , denotes ,
T11013 24222-24225 CC denotes and
T11014 24226-24229 PRP$ denotes its
T11015 24230-24234 NN denotes role
T11016 24320-24328 VBN denotes required
T11017 24235-24237 IN denotes in
T11018 24238-24254 JJ denotes nonhematopoietic
T11019 24255-24262 NNS denotes tissues
T11020 24262-24264 , denotes ,
T11021 24264-24268 JJ denotes such
T11022 24269-24271 IN denotes as
T11023 24272-24277 NN denotes trunk
T11024 24278-24288 NN denotes mesenchyme
T11025 24289-24291 CC denotes or
T11026 24292-24295 DT denotes the
T11027 24304-24310 NN denotes system
T11028 24296-24303 JJ denotes nervous
T11029 24310-24312 , denotes ,
T11030 24312-24314 VBZ denotes is
T11031 24315-24319 RB denotes also
T11032 24329-24332 IN denotes for
T11033 24333-24339 NN denotes embryo
T11034 24340-24348 NN denotes survival
T11035 24348-24349 . denotes .
T11963 24924-24929 NN denotes Tif1γ
T11964 24974-24983 VBN denotes Regulated
T11965 24930-24932 IN denotes in
T11966 24933-24941 NN denotes Punctate
T11967 24950-24954 NNS denotes Foci
T11968 24942-24949 JJ denotes Nuclear
T11969 24955-24957 VBZ denotes Is
T11970 24958-24973 RB denotes Developmentally
T11971 24983-25201 sentence denotes In order to examine the subcellular distribution of Tif1γ protein, we generated an affinity-purified rabbit polyclonal antiserum directed against the C-terminal 15 amino acids conserved in human TIF1γ and mouse Tif1γ.
T11972 24984-24986 IN denotes In
T11973 25054-25063 VBD denotes generated
T11974 24987-24992 NN denotes order
T11975 24993-24995 TO denotes to
T11976 24996-25003 VB denotes examine
T11977 25004-25007 DT denotes the
T11978 25020-25032 NN denotes distribution
T11979 25008-25019 JJ denotes subcellular
T11980 25033-25035 IN denotes of
T11981 25036-25041 NN denotes Tif1γ
T11982 25042-25049 NN denotes protein
T11983 25049-25051 , denotes ,
T11984 25051-25053 PRP denotes we
T11985 25064-25066 DT denotes an
T11986 25103-25112 NN denotes antiserum
T11987 25067-25075 NN denotes affinity
T11988 25076-25084 VBN denotes purified
T11989 25075-25076 HYPH denotes -
T11990 25085-25091 NN denotes rabbit
T11991 25092-25102 JJ denotes polyclonal
T11992 25113-25121 VBN denotes directed
T11993 25122-25129 IN denotes against
T11994 25130-25133 DT denotes the
T11995 25154-25159 NNS denotes acids
T11996 25134-25135 NN denotes C
T11997 25136-25144 JJ denotes terminal
T11998 25135-25136 HYPH denotes -
T11999 25145-25147 CD denotes 15
T12000 25148-25153 NN denotes amino
T12001 25160-25169 VBN denotes conserved
T12002 25170-25172 IN denotes in
T12003 25173-25178 JJ denotes human
T12004 25179-25184 NN denotes TIF1γ
T12005 25185-25188 CC denotes and
T12006 25189-25194 NN denotes mouse
T12007 25195-25200 NN denotes Tif1γ
T12008 25200-25201 . denotes .
T12009 25201-25356 sentence denotes Immunofluorescence of mouse embryo fibroblast nuclei with the anti-Tif1γ antiserum demonstrates that Tif1γ is localized in small nuclear foci (Figure 6A).
T12010 25202-25220 NN denotes Immunofluorescence
T12011 25285-25297 VBZ denotes demonstrates
T12012 25221-25223 IN denotes of
T12013 25224-25229 NN denotes mouse
T12014 25230-25236 NN denotes embryo
T12015 25237-25247 NN denotes fibroblast
T12016 25248-25254 NNS denotes nuclei
T12017 25255-25259 IN denotes with
T12018 25260-25263 DT denotes the
T12019 25275-25284 NN denotes antiserum
T12020 25264-25274 JJ denotes anti-Tif1γ
T12021 25298-25302 IN denotes that
T12022 25312-25321 VBN denotes localized
T12023 25303-25308 NN denotes Tif1γ
T12024 25309-25311 VBZ denotes is
T12025 25322-25324 IN denotes in
T12026 25325-25330 JJ denotes small
T12027 25339-25343 NNS denotes foci
T12028 25331-25338 JJ denotes nuclear
T12029 25344-25345 -LRB- denotes (
T12030 25345-25351 NN denotes Figure
T12031 25352-25354 CD denotes 6A
T12032 25354-25355 -RRB- denotes )
T12033 25355-25356 . denotes .
T12034 25356-25539 sentence denotes The localization of Tif1γ protein appears different from the more diffuse nuclear patterns typically seen in studies of Tif1α (Remboutsika et al. 2002) or TIF1β (Cammas et al. 2002).
T12035 25357-25360 DT denotes The
T12036 25361-25373 NN denotes localization
T12037 25391-25398 VBZ denotes appears
T12038 25374-25376 IN denotes of
T12039 25377-25382 NN denotes Tif1γ
T12040 25383-25390 NN denotes protein
T12041 25399-25408 JJ denotes different
T12042 25409-25413 IN denotes from
T12043 25414-25417 DT denotes the
T12044 25439-25447 NNS denotes patterns
T12045 25418-25422 RBR denotes more
T12046 25423-25430 JJ denotes diffuse
T12047 25431-25438 JJ denotes nuclear
T12048 25448-25457 RB denotes typically
T12049 25458-25462 VBN denotes seen
T12050 25463-25465 IN denotes in
T12051 25466-25473 NNS denotes studies
T12052 25474-25476 IN denotes of
T12053 25477-25482 NN denotes Tif1α
T12054 25483-25484 -LRB- denotes (
T12055 25484-25495 NNP denotes Remboutsika
T12056 25496-25498 FW denotes et
T12057 25499-25502 FW denotes al.
T12058 25503-25507 CD denotes 2002
T12059 25507-25508 -RRB- denotes )
T12060 25509-25511 CC denotes or
T12061 25512-25517 NN denotes TIF1β
T12062 25518-25519 -LRB- denotes (
T12063 25519-25525 NNP denotes Cammas
T12064 25526-25528 FW denotes et
T12065 25529-25532 FW denotes al.
T12066 25533-25537 CD denotes 2002
T12067 25537-25538 -RRB- denotes )
T12068 25538-25539 . denotes .
T12069 25539-25699 sentence denotes A recent report demonstrates that TIF1β associates with heterochromatin-containing foci after retinoic acid treatment or serum starvation (Cammas et al. 2002).
T12070 25540-25541 DT denotes A
T12071 25549-25555 NN denotes report
T12072 25542-25548 JJ denotes recent
T12073 25556-25568 VBZ denotes demonstrates
T12074 25569-25573 IN denotes that
T12075 25580-25590 VBZ denotes associates
T12076 25574-25579 NN denotes TIF1β
T12077 25591-25595 IN denotes with
T12078 25596-25611 NN denotes heterochromatin
T12079 25612-25622 VBG denotes containing
T12080 25611-25612 HYPH denotes -
T12081 25623-25627 NNS denotes foci
T12082 25628-25633 IN denotes after
T12083 25634-25642 JJ denotes retinoic
T12084 25643-25647 NN denotes acid
T12085 25648-25657 NN denotes treatment
T12086 25658-25660 CC denotes or
T12087 25661-25666 NN denotes serum
T12088 25667-25677 NN denotes starvation
T12089 25678-25679 -LRB- denotes (
T12090 25679-25685 NNP denotes Cammas
T12091 25686-25688 FW denotes et
T12092 25689-25692 FW denotes al.
T12093 25693-25697 CD denotes 2002
T12094 25697-25698 -RRB- denotes )
T12095 25698-25699 . denotes .
T12096 25699-25836 sentence denotes Thus, localization or expression of the TIF1 proteins may be regulated during distinct developmental processes or by environmental cues.
T12097 25700-25704 RB denotes Thus
T12098 25761-25770 VBN denotes regulated
T12099 25704-25706 , denotes ,
T12100 25706-25718 NN denotes localization
T12101 25719-25721 CC denotes or
T12102 25722-25732 NN denotes expression
T12103 25733-25735 IN denotes of
T12104 25736-25739 DT denotes the
T12105 25745-25753 NN denotes proteins
T12106 25740-25744 NN denotes TIF1
T12107 25754-25757 MD denotes may
T12108 25758-25760 VB denotes be
T12109 25771-25777 IN denotes during
T12110 25778-25786 JJ denotes distinct
T12111 25801-25810 NNS denotes processes
T12112 25787-25800 JJ denotes developmental
T12113 25811-25813 CC denotes or
T12114 25814-25816 IN denotes by
T12115 25817-25830 JJ denotes environmental
T12116 25831-25835 NNS denotes cues
T12117 25835-25836 . denotes .
T12118 25836-25978 sentence denotes The nuclear foci that contain Tif1γ do not colocalize with two markers of heterochromatin, HP1α protein and DAPI staining of DNA (Figure 6A).
T12119 25837-25840 DT denotes The
T12120 25849-25853 NNS denotes foci
T12121 25841-25848 JJ denotes nuclear
T12122 25880-25890 VB denotes colocalize
T12123 25854-25858 WDT denotes that
T12124 25859-25866 VBP denotes contain
T12125 25867-25872 NN denotes Tif1γ
T12126 25873-25875 VBP denotes do
T12127 25876-25879 RB denotes not
T12128 25891-25895 IN denotes with
T12129 25896-25899 CD denotes two
T12130 25900-25907 NNS denotes markers
T12131 25908-25910 IN denotes of
T12132 25911-25926 NN denotes heterochromatin
T12133 25926-25928 , denotes ,
T12134 25928-25932 NN denotes HP1α
T12135 25933-25940 NN denotes protein
T12136 25941-25944 CC denotes and
T12137 25945-25949 NN denotes DAPI
T12138 25950-25958 NN denotes staining
T12139 25959-25961 IN denotes of
T12140 25962-25965 NN denotes DNA
T12141 25966-25967 -LRB- denotes (
T12142 25967-25973 NN denotes Figure
T12143 25974-25976 CD denotes 6A
T12144 25976-25977 -RRB- denotes )
T12145 25977-25978 . denotes .
T12146 25978-26188 sentence denotes Furthermore, Tif1γ does not colocalize with promyelocytic leukemia gene product (PML) nuclear bodies, DNA repair complexes that contain Mre11, or transcriptional complexes containing TFII-B (unpublished data).
T12147 25979-25990 RB denotes Furthermore
T12148 26007-26017 VB denotes colocalize
T12149 25990-25992 , denotes ,
T12150 25992-25997 NN denotes Tif1γ
T12151 25998-26002 VBZ denotes does
T12152 26003-26006 RB denotes not
T12153 26018-26022 IN denotes with
T12154 26023-26036 JJ denotes promyelocytic
T12155 26051-26058 NN denotes product
T12156 26037-26045 NN denotes leukemia
T12157 26046-26050 NN denotes gene
T12158 26073-26079 NNS denotes bodies
T12159 26059-26060 -LRB- denotes (
T12160 26060-26063 NN denotes PML
T12161 26063-26064 -RRB- denotes )
T12162 26065-26072 JJ denotes nuclear
T12163 26079-26081 , denotes ,
T12164 26081-26084 NN denotes DNA
T12165 26092-26101 NNS denotes complexes
T12166 26085-26091 NN denotes repair
T12167 26102-26106 WDT denotes that
T12168 26107-26114 VBP denotes contain
T12169 26115-26120 NN denotes Mre11
T12170 26120-26122 , denotes ,
T12171 26122-26124 CC denotes or
T12172 26125-26140 JJ denotes transcriptional
T12173 26141-26150 NNS denotes complexes
T12174 26151-26161 VBG denotes containing
T12175 26162-26166 NN denotes TFII
T12176 26167-26168 NN denotes B
T12177 26166-26167 HYPH denotes -
T12178 26169-26170 -LRB- denotes (
T12179 26182-26186 NNS denotes data
T12180 26170-26181 JJ denotes unpublished
T12181 26186-26187 -RRB- denotes )
T12182 26187-26188 . denotes .
T12183 26188-26479 sentence denotes We next examined the expression of Tif1γ protein during the differentiation of G1E cells, a murine erythroleukemia cell line that can terminally differentiate into erythrocytes when a Gata1:estrogen receptor fusion protein is stabilized in response to estrogen exposure (Weiss et al. 1997).
T12184 26189-26191 PRP denotes We
T12185 26197-26205 VBD denotes examined
T12186 26192-26196 RB denotes next
T12187 26206-26209 DT denotes the
T12188 26210-26220 NN denotes expression
T12189 26221-26223 IN denotes of
T12190 26224-26229 NN denotes Tif1γ
T12191 26230-26237 NN denotes protein
T12192 26238-26244 IN denotes during
T12193 26245-26248 DT denotes the
T12194 26249-26264 NN denotes differentiation
T12195 26265-26267 IN denotes of
T12196 26268-26271 NN denotes G1E
T12197 26272-26277 NNS denotes cells
T12198 26277-26279 , denotes ,
T12199 26279-26280 DT denotes a
T12200 26309-26313 NN denotes line
T12201 26281-26287 JJ denotes murine
T12202 26288-26303 NN denotes erythroleukemia
T12203 26304-26308 NN denotes cell
T12204 26314-26318 WDT denotes that
T12205 26334-26347 VB denotes differentiate
T12206 26319-26322 MD denotes can
T12207 26323-26333 RB denotes terminally
T12208 26348-26352 IN denotes into
T12209 26353-26365 NNS denotes erythrocytes
T12210 26366-26370 WRB denotes when
T12211 26415-26425 VBN denotes stabilized
T12212 26371-26372 DT denotes a
T12213 26404-26411 NN denotes protein
T12214 26373-26378 NN denotes Gata1
T12215 26379-26387 NN denotes estrogen
T12216 26378-26379 : denotes :
T12217 26388-26396 NN denotes receptor
T12218 26397-26403 NN denotes fusion
T12219 26412-26414 VBZ denotes is
T12220 26426-26428 IN denotes in
T12221 26429-26437 NN denotes response
T12222 26438-26440 IN denotes to
T12223 26441-26449 NN denotes estrogen
T12224 26450-26458 NN denotes exposure
T12225 26459-26460 -LRB- denotes (
T12226 26460-26465 NNP denotes Weiss
T12227 26466-26468 FW denotes et
T12228 26469-26472 FW denotes al.
T12229 26473-26477 CD denotes 1997
T12230 26477-26478 -RRB- denotes )
T12231 26478-26479 . denotes .
T12232 26479-26607 sentence denotes Western blot analysis demonstrated that Tif1γ protein expression decreases with terminal erythroid differentiation (Figure 6B).
T12233 26480-26487 NNP denotes Western
T12234 26488-26492 NN denotes blot
T12235 26493-26501 NN denotes analysis
T12236 26502-26514 VBD denotes demonstrated
T12237 26515-26519 IN denotes that
T12238 26545-26554 VBZ denotes decreases
T12239 26520-26525 NN denotes Tif1γ
T12240 26534-26544 NN denotes expression
T12241 26526-26533 NN denotes protein
T12242 26555-26559 IN denotes with
T12243 26560-26568 JJ denotes terminal
T12244 26569-26578 NN denotes erythroid
T12245 26579-26594 NN denotes differentiation
T12246 26595-26596 -LRB- denotes (
T12247 26596-26602 NN denotes Figure
T12248 26603-26605 CD denotes 6B
T12249 26605-26606 -RRB- denotes )
T12250 26606-26607 . denotes .
T12251 26607-26771 sentence denotes Consistent with this finding, after 24 hpf, zebrafish mon mRNA expression falls during the terminal maturation of the primitive erythroid cells (unpublished data).
T12252 26608-26618 JJ denotes Consistent
T12253 26682-26687 VBZ denotes falls
T12254 26619-26623 IN denotes with
T12255 26624-26628 DT denotes this
T12256 26629-26636 NN denotes finding
T12257 26636-26638 , denotes ,
T12258 26638-26643 IN denotes after
T12259 26644-26646 CD denotes 24
T12260 26647-26650 NN denotes hpf
T12261 26650-26652 , denotes ,
T12262 26652-26661 NN denotes zebrafish
T12263 26666-26670 NN denotes mRNA
T12264 26662-26665 NN denotes mon
T12265 26671-26681 NN denotes expression
T12266 26688-26694 IN denotes during
T12267 26695-26698 DT denotes the
T12268 26708-26718 NN denotes maturation
T12269 26699-26707 JJ denotes terminal
T12270 26719-26721 IN denotes of
T12271 26722-26725 DT denotes the
T12272 26746-26751 NNS denotes cells
T12273 26726-26735 JJ denotes primitive
T12274 26736-26745 JJ denotes erythroid
T12275 26752-26753 -LRB- denotes (
T12276 26765-26769 NNS denotes data
T12277 26753-26764 JJ denotes unpublished
T12278 26769-26770 -RRB- denotes )
T12279 26770-26771 . denotes .
T12280 26771-27026 sentence denotes In two different murine erythroleukemia cell lines (MEL and G1E), Tif1γ is also expressed in nuclear foci, and even though the overall Tif1γ protein level is reduced, this nuclear foci localization does not change with differentiation (unpublished data).
T12281 26772-26774 IN denotes In
T12282 26852-26861 VBN denotes expressed
T12283 26775-26778 CD denotes two
T12284 26817-26822 NNS denotes lines
T12285 26779-26788 JJ denotes different
T12286 26789-26795 JJ denotes murine
T12287 26796-26811 NN denotes erythroleukemia
T12288 26812-26816 NN denotes cell
T12289 26823-26824 -LRB- denotes (
T12290 26824-26827 NN denotes MEL
T12291 26828-26831 CC denotes and
T12292 26832-26835 NN denotes G1E
T12293 26835-26836 -RRB- denotes )
T12294 26836-26838 , denotes ,
T12295 26838-26843 NN denotes Tif1γ
T12296 26844-26846 VBZ denotes is
T12297 26847-26851 RB denotes also
T12298 26862-26864 IN denotes in
T12299 26865-26872 JJ denotes nuclear
T12300 26873-26877 NNS denotes foci
T12301 26877-26879 , denotes ,
T12302 26879-26882 CC denotes and
T12303 26883-26887 RB denotes even
T12304 26930-26937 VBN denotes reduced
T12305 26888-26894 IN denotes though
T12306 26895-26898 DT denotes the
T12307 26921-26926 NN denotes level
T12308 26899-26906 JJ denotes overall
T12309 26907-26912 NN denotes Tif1γ
T12310 26913-26920 NN denotes protein
T12311 26927-26929 VBZ denotes is
T12312 26979-26985 VB denotes change
T12313 26937-26939 , denotes ,
T12314 26939-26943 DT denotes this
T12315 26957-26969 NN denotes localization
T12316 26944-26951 JJ denotes nuclear
T12317 26952-26956 NNS denotes foci
T12318 26970-26974 VBZ denotes does
T12319 26975-26978 RB denotes not
T12320 26986-26990 IN denotes with
T12321 26991-27006 NN denotes differentiation
T12322 27007-27008 -LRB- denotes (
T12323 27020-27024 NNS denotes data
T12324 27008-27019 JJ denotes unpublished
T12325 27024-27025 -RRB- denotes )
T12326 27025-27026 . denotes .
T12327 27026-27152 sentence denotes This provides further support for the hypothesis that Tif1γ acts within novel nuclear foci, during erythroid differentiation.
T12328 27027-27031 DT denotes This
T12329 27032-27040 VBZ denotes provides
T12330 27041-27048 JJ denotes further
T12331 27049-27056 NN denotes support
T12332 27057-27060 IN denotes for
T12333 27061-27064 DT denotes the
T12334 27065-27075 NN denotes hypothesis
T12335 27076-27080 IN denotes that
T12336 27087-27091 VBZ denotes acts
T12337 27081-27086 NN denotes Tif1γ
T12338 27092-27098 IN denotes within
T12339 27099-27104 JJ denotes novel
T12340 27113-27117 NNS denotes foci
T12341 27105-27112 JJ denotes nuclear
T12342 27117-27119 , denotes ,
T12343 27119-27125 IN denotes during
T12344 27126-27135 JJ denotes erythroid
T12345 27136-27151 NN denotes differentiation
T12346 27151-27152 . denotes .
T12542 0-3 DT denotes The
T12543 4-13 NN denotes Zebrafish
T12544 27909-27911 VBZ denotes is
T12545 27912-27914 DT denotes an
T12546 27931-27937 NN denotes system
T12547 27915-27924 JJ denotes excellent
T12548 27925-27930 NN denotes model
T12549 27938-27940 TO denotes to
T12550 27941-27950 VB denotes elucidate
T12551 27951-27954 DT denotes the
T12552 27965-27974 NN denotes machinery
T12553 27955-27964 JJ denotes molecular
T12554 27975-27986 VBG denotes controlling
T12555 27987-27991 NN denotes gene
T12556 27992-28002 NN denotes expression
T12557 28003-28009 IN denotes during
T12558 28010-28023 NN denotes hematopoiesis
T12559 28024-28025 -LRB- denotes (
T12560 28025-28031 NNP denotes Thisse
T12561 28032-28035 CC denotes and
T12562 28036-28039 NNP denotes Zon
T12563 28040-28044 CD denotes 2002
T12564 28044-28045 : denotes ;
T12565 28046-28054 NNP denotes Galloway
T12566 28055-28058 CC denotes and
T12567 28059-28062 NNP denotes Zon
T12568 28063-28067 CD denotes 2003
T12569 28067-28068 -RRB- denotes )
T12570 28068-28069 . denotes .
T12571 28069-28265 sentence denotes As part of a large-scale forward genetic screen, we originally identified a complementation group of independent mutant alleles in the zebrafish gene that we named moonshine (Ransom et al. 1996).
T12572 28070-28072 IN denotes As
T12573 28133-28143 VBD denotes identified
T12574 28073-28077 NN denotes part
T12575 28078-28080 IN denotes of
T12576 28081-28082 DT denotes a
T12577 28111-28117 NN denotes screen
T12578 28083-28088 JJ denotes large
T12579 28089-28094 NN denotes scale
T12580 28088-28089 HYPH denotes -
T12581 28095-28102 JJ denotes forward
T12582 28103-28110 JJ denotes genetic
T12583 28117-28119 , denotes ,
T12584 28119-28121 PRP denotes we
T12585 28122-28132 RB denotes originally
T12586 28144-28145 DT denotes a
T12587 28162-28167 NN denotes group
T12588 28146-28161 NN denotes complementation
T12589 28168-28170 IN denotes of
T12590 28171-28182 JJ denotes independent
T12591 28190-28197 NNS denotes alleles
T12592 28183-28189 NN denotes mutant
T12593 28198-28200 IN denotes in
T12594 28201-28204 DT denotes the
T12595 28215-28219 NN denotes gene
T12596 28205-28214 NN denotes zebrafish
T12597 28220-28224 WDT denotes that
T12598 28228-28233 VBD denotes named
T12599 28225-28227 PRP denotes we
T12600 28234-28243 NN denotes moonshine
T12601 28244-28245 -LRB- denotes (
T12602 28245-28251 NNP denotes Ransom
T12603 28252-28254 FW denotes et
T12604 28255-28258 FW denotes al.
T12605 28259-28263 CD denotes 1996
T12606 28263-28264 -RRB- denotes )
T12607 28264-28265 . denotes .
T12608 28265-28432 sentence denotes Positional cloning was used to identify the mon gene, establishing a critical role for a transcriptional intermediary factor, Tif1γ, during hematopoietic development.
T12609 28266-28276 JJ denotes Positional
T12610 28277-28284 NN denotes cloning
T12611 28289-28293 VBN denotes used
T12612 28285-28288 VBD denotes was
T12613 28294-28296 TO denotes to
T12614 28297-28305 VB denotes identify
T12615 28306-28309 DT denotes the
T12616 28314-28318 NN denotes gene
T12617 28310-28313 NN denotes mon
T12618 28318-28320 , denotes ,
T12619 28320-28332 VBG denotes establishing
T12620 28333-28334 DT denotes a
T12621 28344-28348 NN denotes role
T12622 28335-28343 JJ denotes critical
T12623 28349-28352 IN denotes for
T12624 28353-28354 DT denotes a
T12625 28384-28390 NN denotes factor
T12626 28355-28370 JJ denotes transcriptional
T12627 28371-28383 JJ denotes intermediary
T12628 28390-28392 , denotes ,
T12629 28392-28397 NN denotes Tif1γ
T12630 28397-28399 , denotes ,
T12631 28399-28405 IN denotes during
T12632 28406-28419 JJ denotes hematopoietic
T12633 28420-28431 NN denotes development
T12634 28431-28432 . denotes .
T13586 28434-28437 DT denotes The
T13587 28442-28446 NN denotes Gene
T13588 28438-28441 NN denotes mon
T13589 28447-28454 VBZ denotes Encodes
T13590 28455-28458 DT denotes the
T13591 28469-28477 NN denotes Ortholog
T13592 28459-28468 NN denotes Zebrafish
T13593 28478-28480 IN denotes of
T13594 28481-28490 JJ denotes Mammalian
T13595 28491-28496 NN denotes TIF1γ
T13596 28496-28650 sentence denotes Our results strongly support the conclusion that we have positionally cloned the zebrafish mon gene correctly, and it is the ortholog of mammalian Tif1γ.
T13597 28497-28500 PRP$ denotes Our
T13598 28501-28508 NNS denotes results
T13599 28518-28525 VBP denotes support
T13600 28509-28517 RB denotes strongly
T13601 28526-28529 DT denotes the
T13602 28530-28540 NN denotes conclusion
T13603 28541-28545 IN denotes that
T13604 28567-28573 VBN denotes cloned
T13605 28546-28548 PRP denotes we
T13606 28549-28553 VBP denotes have
T13607 28554-28566 RB denotes positionally
T13608 28574-28577 DT denotes the
T13609 28592-28596 NN denotes gene
T13610 28578-28587 NN denotes zebrafish
T13611 28588-28591 NN denotes mon
T13612 28597-28606 RB denotes correctly
T13613 28606-28608 , denotes ,
T13614 28608-28611 CC denotes and
T13615 28612-28614 PRP denotes it
T13616 28615-28617 VBZ denotes is
T13617 28618-28621 DT denotes the
T13618 28622-28630 NN denotes ortholog
T13619 28631-28633 IN denotes of
T13620 28634-28643 JJ denotes mammalian
T13621 28644-28649 NN denotes Tif1γ
T13622 28649-28650 . denotes .
T13623 28650-28796 sentence denotes Tif1γ is present in the critical genetic interval encompassing a single approximately 50-kb PAC clone defined by linkage analysis (see Figure 3).
T13624 28651-28656 NN denotes Tif1γ
T13625 28657-28659 VBZ denotes is
T13626 28660-28667 JJ denotes present
T13627 28668-28670 IN denotes in
T13628 28671-28674 DT denotes the
T13629 28692-28700 NN denotes interval
T13630 28675-28683 JJ denotes critical
T13631 28684-28691 JJ denotes genetic
T13632 28701-28713 VBG denotes encompassing
T13633 28714-28715 DT denotes a
T13634 28747-28752 NN denotes clone
T13635 28716-28722 JJ denotes single
T13636 28723-28736 RB denotes approximately
T13637 28737-28739 CD denotes 50
T13638 28740-28742 NN denotes kb
T13639 28739-28740 HYPH denotes -
T13640 28743-28746 NN denotes PAC
T13641 28753-28760 VBN denotes defined
T13642 28761-28763 IN denotes by
T13643 28764-28771 NN denotes linkage
T13644 28772-28780 NN denotes analysis
T13645 28781-28782 -LRB- denotes (
T13646 28782-28785 VB denotes see
T13647 28786-28792 NN denotes Figure
T13648 28793-28794 CD denotes 3
T13649 28794-28795 -RRB- denotes )
T13650 28795-28796 . denotes .
T13651 28796-28981 sentence denotes Sequence analysis indicates that zebrafish tif1γ is most similar in predicted amino acid sequence and intron/exon structure compared to the predicted orthologous human and mouse genes.
T13652 28797-28805 NN denotes Sequence
T13653 28806-28814 NN denotes analysis
T13654 28815-28824 VBZ denotes indicates
T13655 28825-28829 IN denotes that
T13656 28846-28848 VBZ denotes is
T13657 28830-28839 NN denotes zebrafish
T13658 28840-28845 NN denotes tif1γ
T13659 28849-28853 RBS denotes most
T13660 28854-28861 JJ denotes similar
T13661 28862-28864 IN denotes in
T13662 28865-28874 VBN denotes predicted
T13663 28886-28894 NN denotes sequence
T13664 28875-28880 NN denotes amino
T13665 28881-28885 NN denotes acid
T13666 28895-28898 CC denotes and
T13667 28899-28905 NN denotes intron
T13668 28906-28910 NN denotes exon
T13669 28905-28906 HYPH denotes /
T13670 28911-28920 NN denotes structure
T13671 28921-28929 VBN denotes compared
T13672 28930-28932 IN denotes to
T13673 28933-28936 DT denotes the
T13674 28975-28980 NNS denotes genes
T13675 28937-28946 JJ denotes predicted
T13676 28947-28958 JJ denotes orthologous
T13677 28959-28964 JJ denotes human
T13678 28965-28968 CC denotes and
T13679 28969-28974 NN denotes mouse
T13680 28980-28981 . denotes .
T13681 28981-29109 sentence denotes Zebrafish tif1γ is located in a region of zebrafish Chromosome 8 syntenic to the region of human Chromosome 1 containing TIF1γ.
T13682 28982-28991 NN denotes Zebrafish
T13683 28992-28997 NN denotes tif1γ
T13684 29001-29008 VBN denotes located
T13685 28998-29000 VBZ denotes is
T13686 29009-29011 IN denotes in
T13687 29012-29013 DT denotes a
T13688 29014-29020 NN denotes region
T13689 29021-29023 IN denotes of
T13690 29024-29033 NN denotes zebrafish
T13691 29034-29044 NN denotes Chromosome
T13692 29045-29046 CD denotes 8
T13693 29047-29055 JJ denotes syntenic
T13694 29056-29058 IN denotes to
T13695 29059-29062 DT denotes the
T13696 29063-29069 NN denotes region
T13697 29070-29072 IN denotes of
T13698 29073-29078 JJ denotes human
T13699 29079-29089 NN denotes Chromosome
T13700 29090-29091 CD denotes 1
T13701 29092-29102 VBG denotes containing
T13702 29103-29108 NN denotes TIF1γ
T13703 29108-29109 . denotes .
T13704 29109-29242 sentence denotes We identified point mutations in tif1γ from three different alleles of mon that each result in premature stop codons and mRNA decay.
T13705 29110-29112 PRP denotes We
T13706 29113-29123 VBD denotes identified
T13707 29124-29129 NN denotes point
T13708 29130-29139 NNS denotes mutations
T13709 29140-29142 IN denotes in
T13710 29143-29148 NN denotes tif1γ
T13711 29149-29153 IN denotes from
T13712 29154-29159 CD denotes three
T13713 29170-29177 NNS denotes alleles
T13714 29160-29169 JJ denotes different
T13715 29178-29180 IN denotes of
T13716 29181-29184 NN denotes mon
T13717 29185-29189 WDT denotes that
T13718 29195-29201 VBP denotes result
T13719 29190-29194 DT denotes each
T13720 29202-29204 IN denotes in
T13721 29205-29214 JJ denotes premature
T13722 29220-29226 NNS denotes codons
T13723 29215-29219 NN denotes stop
T13724 29227-29230 CC denotes and
T13725 29231-29235 NN denotes mRNA
T13726 29236-29241 NN denotes decay
T13727 29241-29242 . denotes .
T13728 29242-29379 sentence denotes In addition, tif1γ/Tif1γ is highly expressed in hematopoietic cells throughout embryogenesis in both zebrafish and mouse (see Figure 4).
T13729 29243-29245 IN denotes In
T13730 29268-29270 VBZ denotes is
T13731 29246-29254 NN denotes addition
T13732 29254-29256 , denotes ,
T13733 29256-29261 NN denotes tif1γ
T13734 29262-29267 NN denotes Tif1γ
T13735 29261-29262 HYPH denotes /
T13736 29271-29277 RB denotes highly
T13737 29278-29287 JJ denotes expressed
T13738 29288-29290 IN denotes in
T13739 29291-29304 JJ denotes hematopoietic
T13740 29305-29310 NNS denotes cells
T13741 29311-29321 IN denotes throughout
T13742 29322-29335 NN denotes embryogenesis
T13743 29336-29338 IN denotes in
T13744 29339-29343 CC denotes both
T13745 29344-29353 NN denotes zebrafish
T13746 29354-29357 CC denotes and
T13747 29358-29363 NN denotes mouse
T13748 29364-29365 -LRB- denotes (
T13749 29365-29368 VB denotes see
T13750 29369-29375 NN denotes Figure
T13751 29376-29377 CD denotes 4
T13752 29377-29378 -RRB- denotes )
T13753 29378-29379 . denotes .
T13754 29379-29560 sentence denotes And as predicted, forced expression of wild-type tif1γ mRNA efficiently rescues hematopoiesis in mon mutants and does not perturb hematopoiesis in wild-type embryos (see Figure 5).
T13755 29380-29383 CC denotes And
T13756 29452-29459 VBZ denotes rescues
T13757 29384-29386 IN denotes as
T13758 29387-29396 VBN denotes predicted
T13759 29396-29398 , denotes ,
T13760 29398-29404 JJ denotes forced
T13761 29405-29415 NN denotes expression
T13762 29416-29418 IN denotes of
T13763 29419-29423 JJ denotes wild
T13764 29424-29428 NN denotes type
T13765 29423-29424 HYPH denotes -
T13766 29435-29439 NN denotes mRNA
T13767 29429-29434 NN denotes tif1γ
T13768 29440-29451 RB denotes efficiently
T13769 29460-29473 NN denotes hematopoiesis
T13770 29474-29476 IN denotes in
T13771 29477-29480 NN denotes mon
T13772 29481-29488 NNS denotes mutants
T13773 29489-29492 CC denotes and
T13774 29493-29497 VBZ denotes does
T13775 29502-29509 VB denotes perturb
T13776 29498-29501 RB denotes not
T13777 29510-29523 NN denotes hematopoiesis
T13778 29524-29526 IN denotes in
T13779 29527-29531 JJ denotes wild
T13780 29532-29536 NN denotes type
T13781 29531-29532 HYPH denotes -
T13782 29537-29544 NNS denotes embryos
T13783 29545-29546 -LRB- denotes (
T13784 29546-29549 VB denotes see
T13785 29550-29556 NN denotes Figure
T13786 29557-29558 CD denotes 5
T13787 29558-29559 -RRB- denotes )
T13788 29559-29560 . denotes .
T13789 29560-29885 sentence denotes We have also cloned the predicted zebrafish ortholog of tif1α, which is more uniformly expressed in zebrafish embryos like mammalian TIF1α (Le Douarin et al. 1995; Niederreither et al. 1999) (see Figures 3A and 4A) and may therefore be available to form hetero-oligomers with Tif1γ protein in developing hematopoietic cells.
T13790 29561-29563 PRP denotes We
T13791 29574-29580 VBN denotes cloned
T13792 29564-29568 VBP denotes have
T13793 29569-29573 RB denotes also
T13794 29581-29584 DT denotes the
T13795 29605-29613 NN denotes ortholog
T13796 29585-29594 JJ denotes predicted
T13797 29595-29604 NN denotes zebrafish
T13798 29614-29616 IN denotes of
T13799 29617-29622 NN denotes tif1α
T13800 29622-29624 , denotes ,
T13801 29624-29629 WDT denotes which
T13802 29648-29657 VBN denotes expressed
T13803 29630-29632 VBZ denotes is
T13804 29633-29637 RBR denotes more
T13805 29638-29647 RB denotes uniformly
T13806 29658-29660 IN denotes in
T13807 29661-29670 NN denotes zebrafish
T13808 29671-29678 NNS denotes embryos
T13809 29679-29683 IN denotes like
T13810 29684-29693 JJ denotes mammalian
T13811 29694-29699 NN denotes TIF1α
T13812 29700-29701 -LRB- denotes (
T13813 29701-29703 NNP denotes Le
T13814 29704-29711 NNP denotes Douarin
T13815 29712-29714 FW denotes et
T13816 29715-29718 FW denotes al.
T13817 29719-29723 CD denotes 1995
T13818 29723-29724 : denotes ;
T13819 29725-29738 NNP denotes Niederreither
T13820 29739-29741 FW denotes et
T13821 29742-29745 FW denotes al.
T13822 29746-29750 CD denotes 1999
T13823 29750-29751 -RRB- denotes )
T13824 29752-29753 -LRB- denotes (
T13825 29753-29756 VB denotes see
T13826 29757-29764 NNS denotes Figures
T13827 29765-29767 CD denotes 3A
T13828 29768-29771 CC denotes and
T13829 29772-29774 CD denotes 4A
T13830 29774-29775 -RRB- denotes )
T13831 29776-29779 CC denotes and
T13832 29780-29783 MD denotes may
T13833 29794-29796 VB denotes be
T13834 29784-29793 RB denotes therefore
T13835 29797-29806 JJ denotes available
T13836 29807-29809 TO denotes to
T13837 29810-29814 VB denotes form
T13838 29815-29831 NNS denotes hetero-oligomers
T13839 29832-29836 IN denotes with
T13840 29837-29842 NN denotes Tif1γ
T13841 29843-29850 NN denotes protein
T13842 29851-29853 IN denotes in
T13843 29854-29864 VBG denotes developing
T13844 29879-29884 NNS denotes cells
T13845 29865-29878 JJ denotes hematopoietic
T13846 29884-29885 . denotes .
T13847 29885-30104 sentence denotes Comparing available zebrafish and mammalian TIF1-predicted amino acid sequences, it appears that the Tif1γ orthologs are the most highly conserved family members while the Tif1α sequences are relatively more divergent.
T13848 29886-29895 VBG denotes Comparing
T13849 29970-29977 VBZ denotes appears
T13850 29896-29905 JJ denotes available
T13851 29956-29965 NNS denotes sequences
T13852 29906-29915 NN denotes zebrafish
T13853 29916-29919 CC denotes and
T13854 29920-29929 JJ denotes mammalian
T13855 29930-29934 NN denotes TIF1
T13856 29935-29944 VBN denotes predicted
T13857 29934-29935 HYPH denotes -
T13858 29945-29950 NN denotes amino
T13859 29951-29955 NN denotes acid
T13860 29965-29967 , denotes ,
T13861 29967-29969 PRP denotes it
T13862 29978-29982 IN denotes that
T13863 30003-30006 VBP denotes are
T13864 29983-29986 DT denotes the
T13865 29993-30002 NNS denotes orthologs
T13866 29987-29992 NN denotes Tif1γ
T13867 30007-30010 DT denotes the
T13868 30040-30047 NNS denotes members
T13869 30011-30015 RBS denotes most
T13870 30023-30032 VBN denotes conserved
T13871 30016-30022 RB denotes highly
T13872 30033-30039 NN denotes family
T13873 30048-30053 IN denotes while
T13874 30074-30077 VBP denotes are
T13875 30054-30057 DT denotes the
T13876 30064-30073 NNS denotes sequences
T13877 30058-30063 NN denotes Tif1α
T13878 30078-30088 RB denotes relatively
T13879 30089-30093 RBR denotes more
T13880 30094-30103 JJ denotes divergent
T13881 30103-30104 . denotes .
T13882 30104-30200 sentence denotes We have not found a Tif1β ortholog, thus far, in the zebrafish or fugu genome or EST sequences.
T13883 30105-30107 PRP denotes We
T13884 30117-30122 VBN denotes found
T13885 30108-30112 VBP denotes have
T13886 30113-30116 RB denotes not
T13887 30123-30124 DT denotes a
T13888 30131-30139 NN denotes ortholog
T13889 30125-30130 NN denotes Tif1β
T13890 30139-30141 , denotes ,
T13891 30141-30145 RB denotes thus
T13892 30146-30149 RB denotes far
T13893 30149-30151 , denotes ,
T13894 30151-30153 IN denotes in
T13895 30154-30157 DT denotes the
T13896 30190-30199 NNS denotes sequences
T13897 30158-30167 NN denotes zebrafish
T13898 30168-30170 CC denotes or
T13899 30171-30175 NN denotes fugu
T13900 30176-30182 NN denotes genome
T13901 30183-30185 CC denotes or
T13902 30186-30189 NN denotes EST
T13903 30199-30200 . denotes .
T13904 30200-30332 sentence denotes It is possible that Tif1β, like the KRAB domain transcription factors it binds to, may be present only in tetrapods (Urrutia 2003).
T13905 30201-30203 PRP denotes It
T13906 30204-30206 VBZ denotes is
T13907 30207-30215 JJ denotes possible
T13908 30216-30220 IN denotes that
T13909 30288-30290 VB denotes be
T13910 30221-30226 NN denotes Tif1β
T13911 30226-30228 , denotes ,
T13912 30228-30232 IN denotes like
T13913 30233-30236 DT denotes the
T13914 30263-30270 NNS denotes factors
T13915 30237-30241 NN denotes KRAB
T13916 30242-30248 NN denotes domain
T13917 30249-30262 NN denotes transcription
T13918 30271-30273 PRP denotes it
T13919 30274-30279 VBZ denotes binds
T13920 30280-30282 IN denotes to
T13921 30282-30284 , denotes ,
T13922 30284-30287 MD denotes may
T13923 30291-30298 JJ denotes present
T13924 30299-30303 RB denotes only
T13925 30304-30306 IN denotes in
T13926 30307-30316 NNS denotes tetrapods
T13927 30317-30318 -LRB- denotes (
T13928 30318-30325 NNP denotes Urrutia
T13929 30326-30330 CD denotes 2003
T13930 30330-30331 -RRB- denotes )
T13931 30331-30332 . denotes .
T13932 30332-30415 sentence denotes However, more complete genome sequences will be needed to confirm this hypothesis.
T13933 30333-30340 RB denotes However
T13934 30381-30387 VBN denotes needed
T13935 30340-30342 , denotes ,
T13936 30342-30346 RBR denotes more
T13937 30347-30355 JJ denotes complete
T13938 30363-30372 NNS denotes sequences
T13939 30356-30362 NN denotes genome
T13940 30373-30377 MD denotes will
T13941 30378-30380 VB denotes be
T13942 30388-30390 TO denotes to
T13943 30391-30398 VB denotes confirm
T13944 30399-30403 DT denotes this
T13945 30404-30414 NN denotes hypothesis
T13946 30414-30415 . denotes .
T13947 30415-30624 sentence denotes Based on our analysis of zebrafish mon mutants, it is reasonable to predict that Tif1γ, the most evolutionarily conserved TIF1 family member, plays a similarly essential role in human and mouse hematopoiesis.
T13948 30416-30421 VBN denotes Based
T13949 30467-30469 VBZ denotes is
T13950 30422-30424 IN denotes on
T13951 30425-30428 PRP$ denotes our
T13952 30429-30437 NN denotes analysis
T13953 30438-30440 IN denotes of
T13954 30441-30450 NN denotes zebrafish
T13955 30455-30462 NNS denotes mutants
T13956 30451-30454 NN denotes mon
T13957 30462-30464 , denotes ,
T13958 30464-30466 PRP denotes it
T13959 30470-30480 JJ denotes reasonable
T13960 30481-30483 TO denotes to
T13961 30484-30491 VB denotes predict
T13962 30492-30496 IN denotes that
T13963 30558-30563 VBZ denotes plays
T13964 30497-30502 NN denotes Tif1γ
T13965 30502-30504 , denotes ,
T13966 30504-30507 DT denotes the
T13967 30550-30556 NN denotes member
T13968 30508-30512 RBS denotes most
T13969 30528-30537 VBN denotes conserved
T13970 30513-30527 RB denotes evolutionarily
T13971 30538-30542 NN denotes TIF1
T13972 30543-30549 NN denotes family
T13973 30556-30558 , denotes ,
T13974 30564-30565 DT denotes a
T13975 30586-30590 NN denotes role
T13976 30566-30575 RB denotes similarly
T13977 30576-30585 JJ denotes essential
T13978 30591-30593 IN denotes in
T13979 30594-30599 JJ denotes human
T13980 30610-30623 NN denotes hematopoiesis
T13981 30600-30603 CC denotes and
T13982 30604-30609 NN denotes mouse
T13983 30623-30624 . denotes .
T15217 30626-30635 NNS denotes Mutations
T15218 30645-30650 VBP denotes Cause
T15219 30636-30638 IN denotes in
T15220 30639-30644 NN denotes tif1γ
T15221 30651-30660 NN denotes Apoptosis
T15222 30661-30663 IN denotes of
T15223 30664-30673 JJ denotes Erythroid
T15224 30674-30685 NNS denotes Progenitors
T15225 30685-30960 sentence denotes Our examination of hematopoietic gene expression, apoptosis, and marrow histology in mon mutants demonstrates that early erythroid progenitors are formed in homozygous mutants, but they fail to properly differentiate and instead undergo programmed cell death (see Figure 1).
T15226 30686-30689 PRP$ denotes Our
T15227 30690-30701 NN denotes examination
T15228 30783-30795 VBZ denotes demonstrates
T15229 30702-30704 IN denotes of
T15230 30705-30718 JJ denotes hematopoietic
T15231 30724-30734 NN denotes expression
T15232 30719-30723 NN denotes gene
T15233 30734-30736 , denotes ,
T15234 30736-30745 NN denotes apoptosis
T15235 30745-30747 , denotes ,
T15236 30747-30750 CC denotes and
T15237 30751-30757 NN denotes marrow
T15238 30758-30767 NN denotes histology
T15239 30768-30770 IN denotes in
T15240 30771-30774 NN denotes mon
T15241 30775-30782 NNS denotes mutants
T15242 30796-30800 IN denotes that
T15243 30833-30839 VBN denotes formed
T15244 30801-30806 JJ denotes early
T15245 30817-30828 NNS denotes progenitors
T15246 30807-30816 JJ denotes erythroid
T15247 30829-30832 VBP denotes are
T15248 30840-30842 IN denotes in
T15249 30843-30853 JJ denotes homozygous
T15250 30854-30861 NNS denotes mutants
T15251 30861-30863 , denotes ,
T15252 30863-30866 CC denotes but
T15253 30867-30871 PRP denotes they
T15254 30872-30876 VBP denotes fail
T15255 30877-30879 TO denotes to
T15256 30889-30902 VB denotes differentiate
T15257 30880-30888 RB denotes properly
T15258 30903-30906 CC denotes and
T15259 30907-30914 RB denotes instead
T15260 30915-30922 VBP denotes undergo
T15261 30923-30933 VBN denotes programmed
T15262 30939-30944 NN denotes death
T15263 30934-30938 NN denotes cell
T15264 30945-30946 -LRB- denotes (
T15265 30946-30949 VB denotes see
T15266 30950-30956 NN denotes Figure
T15267 30957-30958 CD denotes 1
T15268 30958-30959 -RRB- denotes )
T15269 30959-30960 . denotes .
T15270 30960-31062 sentence denotes The expression of gata1 appears to initiate normally in the committed erythroid cells of mon mutants.
T15271 30961-30964 DT denotes The
T15272 30965-30975 NN denotes expression
T15273 30985-30992 VBZ denotes appears
T15274 30976-30978 IN denotes of
T15275 30979-30984 NN denotes gata1
T15276 30993-30995 TO denotes to
T15277 30996-31004 VB denotes initiate
T15278 31005-31013 RB denotes normally
T15279 31014-31016 IN denotes in
T15280 31017-31020 DT denotes the
T15281 31041-31046 NNS denotes cells
T15282 31021-31030 VBN denotes committed
T15283 31031-31040 JJ denotes erythroid
T15284 31047-31049 IN denotes of
T15285 31050-31053 NN denotes mon
T15286 31054-31061 NNS denotes mutants
T15287 31061-31062 . denotes .
T15288 31062-31142 sentence denotes However, the cells are abnormal prior to the complete loss of gata1 expression.
T15289 31063-31070 RB denotes However
T15290 31082-31085 VBP denotes are
T15291 31070-31072 , denotes ,
T15292 31072-31075 DT denotes the
T15293 31076-31081 NNS denotes cells
T15294 31086-31094 JJ denotes abnormal
T15295 31095-31100 JJ denotes prior
T15296 31101-31103 IN denotes to
T15297 31104-31107 DT denotes the
T15298 31117-31121 NN denotes loss
T15299 31108-31116 JJ denotes complete
T15300 31122-31124 IN denotes of
T15301 31125-31130 NN denotes gata1
T15302 31131-31141 NN denotes expression
T15303 31141-31142 . denotes .
T15304 31142-31290 sentence denotes TUNEL-positive apoptotic cells are abundant by the 12-somite stage of development, and by 22 hpf all hematopoietic gene expression is extinguished.
T15305 31143-31148 NN denotes TUNEL
T15306 31149-31157 JJ denotes positive
T15307 31148-31149 HYPH denotes -
T15308 31168-31173 NNS denotes cells
T15309 31158-31167 JJ denotes apoptotic
T15310 31174-31177 VBP denotes are
T15311 31178-31186 JJ denotes abundant
T15312 31187-31189 IN denotes by
T15313 31190-31193 DT denotes the
T15314 31204-31209 NN denotes stage
T15315 31194-31196 CD denotes 12
T15316 31197-31203 NN denotes somite
T15317 31196-31197 HYPH denotes -
T15318 31210-31212 IN denotes of
T15319 31213-31224 NN denotes development
T15320 31224-31226 , denotes ,
T15321 31226-31229 CC denotes and
T15322 31230-31232 IN denotes by
T15323 31277-31289 VBN denotes extinguished
T15324 31233-31235 CD denotes 22
T15325 31236-31239 NN denotes hpf
T15326 31240-31243 DT denotes all
T15327 31263-31273 NN denotes expression
T15328 31244-31257 JJ denotes hematopoietic
T15329 31258-31262 NN denotes gene
T15330 31274-31276 VBZ denotes is
T15331 31289-31290 . denotes .
T15332 31290-31506 sentence denotes The expression of marker genes, including scl and gata2, characteristic of hematopoietic stem cells and primitive hematopoietic progenitors, are also not detected in the embryonic blood islands of mutants at 22 hpf.
T15333 31291-31294 DT denotes The
T15334 31295-31305 NN denotes expression
T15335 31445-31453 VBN denotes detected
T15336 31306-31308 IN denotes of
T15337 31309-31315 NN denotes marker
T15338 31316-31321 NNS denotes genes
T15339 31321-31323 , denotes ,
T15340 31323-31332 VBG denotes including
T15341 31333-31336 NN denotes scl
T15342 31337-31340 CC denotes and
T15343 31341-31346 NN denotes gata2
T15344 31346-31348 , denotes ,
T15345 31348-31362 JJ denotes characteristic
T15346 31363-31365 IN denotes of
T15347 31366-31379 JJ denotes hematopoietic
T15348 31385-31390 NNS denotes cells
T15349 31380-31384 NN denotes stem
T15350 31391-31394 CC denotes and
T15351 31395-31404 JJ denotes primitive
T15352 31419-31430 NNS denotes progenitors
T15353 31405-31418 JJ denotes hematopoietic
T15354 31430-31432 , denotes ,
T15355 31432-31435 VBP denotes are
T15356 31436-31440 RB denotes also
T15357 31441-31444 RB denotes not
T15358 31454-31456 IN denotes in
T15359 31457-31460 DT denotes the
T15360 31477-31484 NNS denotes islands
T15361 31461-31470 JJ denotes embryonic
T15362 31471-31476 NN denotes blood
T15363 31485-31487 IN denotes of
T15364 31488-31495 NNS denotes mutants
T15365 31496-31498 IN denotes at
T15366 31499-31501 CD denotes 22
T15367 31502-31505 NN denotes hpf
T15368 31505-31506 . denotes .
T15369 31506-31776 sentence denotes This indicates that the mutant hematopoietic cells are not blocked prior to commitment to the erythroid lineage, but instead develop as abnormal erythroid cells and undergo apoptosis, similar to gata1-deficient erythroid cells (Fujiwara et al. 1996; Lyons et al. 2002).
T15370 31507-31511 DT denotes This
T15371 31512-31521 VBZ denotes indicates
T15372 31522-31526 IN denotes that
T15373 31566-31573 VBN denotes blocked
T15374 31527-31530 DT denotes the
T15375 31552-31557 NNS denotes cells
T15376 31531-31537 JJ denotes mutant
T15377 31538-31551 JJ denotes hematopoietic
T15378 31558-31561 VBP denotes are
T15379 31562-31565 RB denotes not
T15380 31574-31579 JJ denotes prior
T15381 31580-31582 IN denotes to
T15382 31583-31593 NN denotes commitment
T15383 31594-31596 IN denotes to
T15384 31597-31600 DT denotes the
T15385 31611-31618 NN denotes lineage
T15386 31601-31610 JJ denotes erythroid
T15387 31618-31620 , denotes ,
T15388 31620-31623 CC denotes but
T15389 31624-31631 RB denotes instead
T15390 31632-31639 VBP denotes develop
T15391 31640-31642 IN denotes as
T15392 31643-31651 JJ denotes abnormal
T15393 31662-31667 NNS denotes cells
T15394 31652-31661 JJ denotes erythroid
T15395 31668-31671 CC denotes and
T15396 31672-31679 VBP denotes undergo
T15397 31680-31689 NN denotes apoptosis
T15398 31689-31691 , denotes ,
T15399 31691-31698 JJ denotes similar
T15400 31699-31701 IN denotes to
T15401 31702-31707 NN denotes gata1
T15402 31728-31733 NNS denotes cells
T15403 31707-31708 HYPH denotes -
T15404 31708-31717 JJ denotes deficient
T15405 31718-31727 JJ denotes erythroid
T15406 31734-31735 -LRB- denotes (
T15407 31735-31743 NNP denotes Fujiwara
T15408 31744-31746 FW denotes et
T15409 31747-31750 FW denotes al.
T15410 31751-31755 CD denotes 1996
T15411 31755-31756 : denotes ;
T15412 31757-31762 NNP denotes Lyons
T15413 31763-31765 FW denotes et
T15414 31766-31769 FW denotes al.
T15415 31770-31774 CD denotes 2002
T15416 31774-31775 -RRB- denotes )
T15417 31775-31776 . denotes .
T15418 31776-31972 sentence denotes Defective erythropoiesis and severe anemia were also observed in rare surviving homozygous mutant mon adults, demonstrating that tif1γ is also required in definitive hematopoiesis (see Figure 2).
T15419 31777-31786 JJ denotes Defective
T15420 31787-31801 NN denotes erythropoiesis
T15421 31830-31838 VBN denotes observed
T15422 31802-31805 CC denotes and
T15423 31806-31812 JJ denotes severe
T15424 31813-31819 NN denotes anemia
T15425 31820-31824 VBD denotes were
T15426 31825-31829 RB denotes also
T15427 31839-31841 IN denotes in
T15428 31842-31846 JJ denotes rare
T15429 31879-31885 NNS denotes adults
T15430 31847-31856 VBG denotes surviving
T15431 31857-31867 JJ denotes homozygous
T15432 31875-31878 NN denotes mon
T15433 31868-31874 JJ denotes mutant
T15434 31885-31887 , denotes ,
T15435 31887-31900 VBG denotes demonstrating
T15436 31901-31905 IN denotes that
T15437 31920-31928 VBN denotes required
T15438 31906-31911 NN denotes tif1γ
T15439 31912-31914 VBZ denotes is
T15440 31915-31919 RB denotes also
T15441 31929-31931 IN denotes in
T15442 31932-31942 JJ denotes definitive
T15443 31943-31956 NN denotes hematopoiesis
T15444 31957-31958 -LRB- denotes (
T15445 31958-31961 VB denotes see
T15446 31962-31968 NN denotes Figure
T15447 31969-31970 CD denotes 2
T15448 31970-31971 -RRB- denotes )
T15449 31971-31972 . denotes .
T15450 31972-32069 sentence denotes The zygotic phenotypes of mon mutants may not reveal the function of maternally inherited Tif1γ.
T15451 31973-31976 DT denotes The
T15452 31985-31995 NNS denotes phenotypes
T15453 31977-31984 JJ denotes zygotic
T15454 32019-32025 VB denotes reveal
T15455 31996-31998 IN denotes of
T15456 31999-32002 NN denotes mon
T15457 32003-32010 NNS denotes mutants
T15458 32011-32014 MD denotes may
T15459 32015-32018 RB denotes not
T15460 32026-32029 DT denotes the
T15461 32030-32038 NN denotes function
T15462 32039-32041 IN denotes of
T15463 32042-32052 RB denotes maternally
T15464 32053-32062 VBN denotes inherited
T15465 32063-32068 NN denotes Tif1γ
T15466 32068-32069 . denotes .
T15467 32069-32272 sentence denotes Maternally expressed zebrafish Tif1γ may play roles in hematopoiesis or other aspects of organogenesis that are not detectable due to the presence of wild-type mRNA in eggs laid by heterozygous mothers.
T15468 32070-32080 RB denotes Maternally
T15469 32081-32090 VBN denotes expressed
T15470 32101-32106 NN denotes Tif1γ
T15471 32091-32100 NN denotes zebrafish
T15472 32111-32115 VB denotes play
T15473 32107-32110 MD denotes may
T15474 32116-32121 NNS denotes roles
T15475 32122-32124 IN denotes in
T15476 32125-32138 NN denotes hematopoiesis
T15477 32139-32141 CC denotes or
T15478 32142-32147 JJ denotes other
T15479 32148-32155 NNS denotes aspects
T15480 32156-32158 IN denotes of
T15481 32159-32172 NN denotes organogenesis
T15482 32173-32177 WDT denotes that
T15483 32178-32181 VBP denotes are
T15484 32182-32185 RB denotes not
T15485 32186-32196 JJ denotes detectable
T15486 32197-32200 IN denotes due
T15487 32201-32203 IN denotes to
T15488 32204-32207 DT denotes the
T15489 32208-32216 NN denotes presence
T15490 32217-32219 IN denotes of
T15491 32220-32224 JJ denotes wild
T15492 32225-32229 NN denotes type
T15493 32224-32225 HYPH denotes -
T15494 32230-32234 NN denotes mRNA
T15495 32235-32237 IN denotes in
T15496 32238-32242 NNS denotes eggs
T15497 32243-32247 VBN denotes laid
T15498 32248-32250 IN denotes by
T15499 32251-32263 JJ denotes heterozygous
T15500 32264-32271 NNS denotes mothers
T15501 32271-32272 . denotes .
T15502 32272-32397 sentence denotes Analysis of the offspring of homozygous mon mutant female zebrafish will aid in defining the function of this maternal mRNA.
T15503 32273-32281 NN denotes Analysis
T15504 32346-32349 VB denotes aid
T15505 32282-32284 IN denotes of
T15506 32285-32288 DT denotes the
T15507 32289-32298 NN denotes offspring
T15508 32299-32301 IN denotes of
T15509 32302-32312 JJ denotes homozygous
T15510 32317-32323 NN denotes mutant
T15511 32313-32316 NN denotes mon
T15512 32331-32340 NN denotes zebrafish
T15513 32324-32330 JJ denotes female
T15514 32341-32345 MD denotes will
T15515 32350-32352 IN denotes in
T15516 32353-32361 VBG denotes defining
T15517 32362-32365 DT denotes the
T15518 32366-32374 NN denotes function
T15519 32375-32377 IN denotes of
T15520 32378-32382 DT denotes this
T15521 32392-32396 NN denotes mRNA
T15522 32383-32391 JJ denotes maternal
T15523 32396-32397 . denotes .
T15524 32397-32611 sentence denotes The present analysis of zygotic mon mutants provides data that are consistent with the conclusion that tif1γ is essential for erythropoiesis but do not rule out essential functions in other hematopoietic lineages.
T15525 32398-32401 DT denotes The
T15526 32410-32418 NN denotes analysis
T15527 32402-32409 JJ denotes present
T15528 32442-32450 VBZ denotes provides
T15529 32419-32421 IN denotes of
T15530 32422-32429 JJ denotes zygotic
T15531 32434-32441 NNS denotes mutants
T15532 32430-32433 NN denotes mon
T15533 32451-32455 NNS denotes data
T15534 32456-32460 WDT denotes that
T15535 32461-32464 VBP denotes are
T15536 32465-32475 JJ denotes consistent
T15537 32476-32480 IN denotes with
T15538 32481-32484 DT denotes the
T15539 32485-32495 NN denotes conclusion
T15540 32496-32500 IN denotes that
T15541 32507-32509 VBZ denotes is
T15542 32501-32506 NN denotes tif1γ
T15543 32510-32519 JJ denotes essential
T15544 32520-32523 IN denotes for
T15545 32524-32538 NN denotes erythropoiesis
T15546 32539-32542 CC denotes but
T15547 32543-32545 VBP denotes do
T15548 32550-32554 VB denotes rule
T15549 32546-32549 RB denotes not
T15550 32555-32558 RP denotes out
T15551 32559-32568 JJ denotes essential
T15552 32569-32578 NNS denotes functions
T15553 32579-32581 IN denotes in
T15554 32582-32587 JJ denotes other
T15555 32602-32610 NNS denotes lineages
T15556 32588-32601 JJ denotes hematopoietic
T15557 32610-32611 . denotes .
T15558 32611-32825 sentence denotes The hematopoietic phenotype of mon mutants resembles the loss of erythroid cells seen in both mouse Gata1 knockout embryos and zebrafish vlad tepes (gata1) mutant embryos (Fujiwara et al. 1996; Lyons et al. 2002).
T15559 32612-32615 DT denotes The
T15560 32630-32639 NN denotes phenotype
T15561 32616-32629 JJ denotes hematopoietic
T15562 32655-32664 VBZ denotes resembles
T15563 32640-32642 IN denotes of
T15564 32643-32646 NN denotes mon
T15565 32647-32654 NNS denotes mutants
T15566 32665-32668 DT denotes the
T15567 32669-32673 NN denotes loss
T15568 32674-32676 IN denotes of
T15569 32677-32686 JJ denotes erythroid
T15570 32687-32692 NNS denotes cells
T15571 32693-32697 VBN denotes seen
T15572 32698-32700 IN denotes in
T15573 32701-32705 CC denotes both
T15574 32727-32734 NNS denotes embryos
T15575 32706-32711 NN denotes mouse
T15576 32718-32726 NN denotes knockout
T15577 32712-32717 NN denotes Gata1
T15578 32735-32738 CC denotes and
T15579 32739-32748 NN denotes zebrafish
T15580 32754-32759 NN denotes tepes
T15581 32749-32753 NN denotes vlad
T15582 32775-32782 NNS denotes embryos
T15583 32760-32761 -LRB- denotes (
T15584 32761-32766 NN denotes gata1
T15585 32766-32767 -RRB- denotes )
T15586 32768-32774 JJ denotes mutant
T15587 32783-32784 -LRB- denotes (
T15588 32784-32792 NNP denotes Fujiwara
T15589 32793-32795 FW denotes et
T15590 32796-32799 FW denotes al.
T15591 32800-32804 CD denotes 1996
T15592 32804-32805 : denotes ;
T15593 32806-32811 NNP denotes Lyons
T15594 32812-32814 FW denotes et
T15595 32815-32818 FW denotes al.
T15596 32819-32823 CD denotes 2002
T15597 32823-32824 -RRB- denotes )
T15598 32824-32825 . denotes .
T15599 32825-32959 sentence denotes In an effort to determine if there is a genetic relationship between mon and gata1, we tested their ability to rescue erythropoiesis.
T15600 32826-32828 IN denotes In
T15601 32913-32919 VBD denotes tested
T15602 32829-32831 DT denotes an
T15603 32832-32838 NN denotes effort
T15604 32839-32841 TO denotes to
T15605 32842-32851 VB denotes determine
T15606 32852-32854 IN denotes if
T15607 32861-32863 VBZ denotes is
T15608 32855-32860 EX denotes there
T15609 32864-32865 DT denotes a
T15610 32874-32886 NN denotes relationship
T15611 32866-32873 JJ denotes genetic
T15612 32887-32894 IN denotes between
T15613 32895-32898 NN denotes mon
T15614 32899-32902 CC denotes and
T15615 32903-32908 NN denotes gata1
T15616 32908-32910 , denotes ,
T15617 32910-32912 PRP denotes we
T15618 32920-32925 PRP$ denotes their
T15619 32926-32933 NN denotes ability
T15620 32934-32936 TO denotes to
T15621 32937-32943 VB denotes rescue
T15622 32944-32958 NN denotes erythropoiesis
T15623 32958-32959 . denotes .
T15624 32959-33132 sentence denotes Both injection of gata1 mRNA into mon homozygous mutant embryos and injection of tif1γ mRNA into gata1 knock-down embryos failed to rescue hematopoiesis (unpublished data).
T15625 32960-32964 CC denotes Both
T15626 32965-32974 NN denotes injection
T15627 33082-33088 VBD denotes failed
T15628 32975-32977 IN denotes of
T15629 32978-32983 NN denotes gata1
T15630 32984-32988 NN denotes mRNA
T15631 32989-32993 IN denotes into
T15632 32994-32997 NN denotes mon
T15633 32998-33008 JJ denotes homozygous
T15634 33016-33023 NNS denotes embryos
T15635 33009-33015 NN denotes mutant
T15636 33024-33027 CC denotes and
T15637 33028-33037 NN denotes injection
T15638 33038-33040 IN denotes of
T15639 33041-33046 NN denotes tif1γ
T15640 33047-33051 NN denotes mRNA
T15641 33052-33056 IN denotes into
T15642 33057-33062 NN denotes gata1
T15643 33074-33081 NNS denotes embryos
T15644 33063-33068 VB denotes knock
T15645 33068-33069 HYPH denotes -
T15646 33069-33073 RP denotes down
T15647 33089-33091 TO denotes to
T15648 33092-33098 VB denotes rescue
T15649 33099-33112 NN denotes hematopoiesis
T15650 33113-33114 -LRB- denotes (
T15651 33126-33130 NNS denotes data
T15652 33114-33125 JJ denotes unpublished
T15653 33130-33131 -RRB- denotes )
T15654 33131-33132 . denotes .
T15655 33132-33303 sentence denotes We also tested for a direct interaction between Tif1γ and Gata1 proteins by coimmunoprecipitation and yeast two-hybrid assays and found no association (unpublished data).
T15656 33133-33135 PRP denotes We
T15657 33141-33147 VBD denotes tested
T15658 33136-33140 RB denotes also
T15659 33148-33151 IN denotes for
T15660 33152-33153 DT denotes a
T15661 33161-33172 NN denotes interaction
T15662 33154-33160 JJ denotes direct
T15663 33173-33180 IN denotes between
T15664 33181-33186 NN denotes Tif1γ
T15665 33197-33205 NN denotes proteins
T15666 33187-33190 CC denotes and
T15667 33191-33196 NN denotes Gata1
T15668 33206-33208 IN denotes by
T15669 33209-33230 NN denotes coimmunoprecipitation
T15670 33252-33258 NNS denotes assays
T15671 33231-33234 CC denotes and
T15672 33235-33240 NN denotes yeast
T15673 33245-33251 NN denotes hybrid
T15674 33241-33244 CD denotes two
T15675 33244-33245 HYPH denotes -
T15676 33259-33262 CC denotes and
T15677 33263-33268 VBD denotes found
T15678 33269-33271 DT denotes no
T15679 33272-33283 NN denotes association
T15680 33284-33285 -LRB- denotes (
T15681 33297-33301 NNS denotes data
T15682 33285-33296 JJ denotes unpublished
T15683 33301-33302 -RRB- denotes )
T15684 33302-33303 . denotes .
T15685 33303-33457 sentence denotes Although the mutations in each of these genes arrest cells at a similar stage of development, our results suggest that gata1 and tif1γ act independently.
T15686 33304-33312 IN denotes Although
T15687 33350-33356 VBP denotes arrest
T15688 33313-33316 DT denotes the
T15689 33317-33326 NNS denotes mutations
T15690 33327-33329 IN denotes in
T15691 33330-33334 DT denotes each
T15692 33335-33337 IN denotes of
T15693 33338-33343 DT denotes these
T15694 33344-33349 NNS denotes genes
T15695 33410-33417 VBP denotes suggest
T15696 33357-33362 NNS denotes cells
T15697 33363-33365 IN denotes at
T15698 33366-33367 DT denotes a
T15699 33376-33381 NN denotes stage
T15700 33368-33375 JJ denotes similar
T15701 33382-33384 IN denotes of
T15702 33385-33396 NN denotes development
T15703 33396-33398 , denotes ,
T15704 33398-33401 PRP$ denotes our
T15705 33402-33409 NNS denotes results
T15706 33418-33422 IN denotes that
T15707 33439-33442 VBP denotes act
T15708 33423-33428 NN denotes gata1
T15709 33429-33432 CC denotes and
T15710 33433-33438 NN denotes tif1γ
T15711 33443-33456 RB denotes independently
T15712 33456-33457 . denotes .
T15713 33457-33622 sentence denotes This does not rule out the possibility that parallel genetic pathways involving gata1 and tif1γ, operating together, regulate gene transcription within blood cells.
T15714 33458-33462 DT denotes This
T15715 33472-33476 VB denotes rule
T15716 33463-33467 VBZ denotes does
T15717 33468-33471 RB denotes not
T15718 33477-33480 RP denotes out
T15719 33481-33484 DT denotes the
T15720 33485-33496 NN denotes possibility
T15721 33497-33501 IN denotes that
T15722 33575-33583 VB denotes regulate
T15723 33502-33510 JJ denotes parallel
T15724 33519-33527 NNS denotes pathways
T15725 33511-33518 JJ denotes genetic
T15726 33528-33537 VBG denotes involving
T15727 33538-33543 NN denotes gata1
T15728 33544-33547 CC denotes and
T15729 33548-33553 NN denotes tif1γ
T15730 33553-33555 , denotes ,
T15731 33555-33564 VBG denotes operating
T15732 33565-33573 RB denotes together
T15733 33573-33575 , denotes ,
T15734 33584-33588 NN denotes gene
T15735 33589-33602 NN denotes transcription
T15736 33603-33609 IN denotes within
T15737 33610-33615 NN denotes blood
T15738 33616-33621 NNS denotes cells
T15739 33621-33622 . denotes .
T16380 33624-33627 DT denotes The
T16381 33628-33632 NN denotes Role
T16382 33633-33635 IN denotes of
T16383 33636-33641 NN denotes Tif1γ
T16384 33642-33644 IN denotes in
T16385 33645-33654 JJ denotes Primitive
T16386 33670-33684 NN denotes Erythropoiesis
T16387 33655-33658 CC denotes and
T16388 33659-33669 JJ denotes Definitive
T16389 33684-33842 sentence denotes Taken together, our data suggest that tif1γ is required as a permissive cofactor for the erythroid lineage-specific control of hematopoietic gene expression.
T16390 33685-33690 VBN denotes Taken
T16391 33710-33717 VBP denotes suggest
T16392 33691-33699 RB denotes together
T16393 33699-33701 , denotes ,
T16394 33701-33704 PRP$ denotes our
T16395 33705-33709 NNS denotes data
T16396 33718-33722 IN denotes that
T16397 33732-33740 VBN denotes required
T16398 33723-33728 NN denotes tif1γ
T16399 33729-33731 VBZ denotes is
T16400 33741-33743 IN denotes as
T16401 33744-33745 DT denotes a
T16402 33757-33765 NN denotes cofactor
T16403 33746-33756 JJ denotes permissive
T16404 33766-33769 IN denotes for
T16405 33770-33773 DT denotes the
T16406 33801-33808 NN denotes control
T16407 33774-33783 JJ denotes erythroid
T16408 33784-33791 NN denotes lineage
T16409 33792-33800 JJ denotes specific
T16410 33791-33792 HYPH denotes -
T16411 33809-33811 IN denotes of
T16412 33812-33825 JJ denotes hematopoietic
T16413 33826-33830 NN denotes gene
T16414 33831-33841 NN denotes expression
T16415 33841-33842 . denotes .
T16416 33842-34169 sentence denotes We reasonably predict that Tif1γ protein functions as a transcriptional intermediary factor in hematopoietic progenitor cells given that both TIF1α (Zhong et al. 1999) and TIF1β (Friedman et al. 1996; Abrink et al. 2001) have been shown to act as intermediary factors that positively or negatively regulate gene transcription.
T16417 33843-33845 PRP denotes We
T16418 33857-33864 VBP denotes predict
T16419 33846-33856 RB denotes reasonably
T16420 33865-33869 IN denotes that
T16421 33884-33893 VBZ denotes functions
T16422 33870-33875 NN denotes Tif1γ
T16423 33876-33883 NN denotes protein
T16424 33894-33896 IN denotes as
T16425 33897-33898 DT denotes a
T16426 33928-33934 NN denotes factor
T16427 33899-33914 JJ denotes transcriptional
T16428 33915-33927 JJ denotes intermediary
T16429 33935-33937 IN denotes in
T16430 33938-33951 JJ denotes hematopoietic
T16431 33963-33968 NNS denotes cells
T16432 33952-33962 NN denotes progenitor
T16433 33969-33974 VBN denotes given
T16434 33975-33979 IN denotes that
T16435 34074-34079 VBN denotes shown
T16436 33980-33984 CC denotes both
T16437 33985-33990 NN denotes TIF1α
T16438 33991-33992 -LRB- denotes (
T16439 33992-33997 NNP denotes Zhong
T16440 33998-34000 FW denotes et
T16441 34001-34004 FW denotes al.
T16442 34005-34009 CD denotes 1999
T16443 34009-34010 -RRB- denotes )
T16444 34011-34014 CC denotes and
T16445 34015-34020 NN denotes TIF1β
T16446 34021-34022 -LRB- denotes (
T16447 34022-34030 NNP denotes Friedman
T16448 34031-34033 FW denotes et
T16449 34034-34037 FW denotes al.
T16450 34038-34042 CD denotes 1996
T16451 34042-34043 : denotes ;
T16452 34044-34050 NNP denotes Abrink
T16453 34051-34053 FW denotes et
T16454 34054-34057 FW denotes al.
T16455 34058-34062 CD denotes 2001
T16456 34062-34063 -RRB- denotes )
T16457 34064-34068 VBP denotes have
T16458 34069-34073 VBN denotes been
T16459 34080-34082 TO denotes to
T16460 34083-34086 VB denotes act
T16461 34087-34089 IN denotes as
T16462 34090-34102 JJ denotes intermediary
T16463 34103-34110 NNS denotes factors
T16464 34111-34115 WDT denotes that
T16465 34141-34149 VBP denotes regulate
T16466 34116-34126 RB denotes positively
T16467 34127-34129 CC denotes or
T16468 34130-34140 RB denotes negatively
T16469 34150-34154 NN denotes gene
T16470 34155-34168 NN denotes transcription
T16471 34168-34169 . denotes .
T16472 34169-34350 sentence denotes These studies indicate that TIF1α and TIF1β act as scaffolds that link different classes of DNA-binding proteins and chromatin-associated proteins into larger regulatory complexes.
T16473 34170-34175 DT denotes These
T16474 34176-34183 NNS denotes studies
T16475 34184-34192 VBP denotes indicate
T16476 34193-34197 IN denotes that
T16477 34214-34217 VBP denotes act
T16478 34198-34203 NN denotes TIF1α
T16479 34204-34207 CC denotes and
T16480 34208-34213 NN denotes TIF1β
T16481 34218-34220 IN denotes as
T16482 34221-34230 NNS denotes scaffolds
T16483 34231-34235 WDT denotes that
T16484 34236-34240 VBP denotes link
T16485 34241-34250 JJ denotes different
T16486 34251-34258 NNS denotes classes
T16487 34259-34261 IN denotes of
T16488 34262-34265 NN denotes DNA
T16489 34266-34273 VBG denotes binding
T16490 34265-34266 HYPH denotes -
T16491 34274-34282 NN denotes proteins
T16492 34283-34286 CC denotes and
T16493 34287-34296 NN denotes chromatin
T16494 34297-34307 VBN denotes associated
T16495 34296-34297 HYPH denotes -
T16496 34308-34316 NN denotes proteins
T16497 34317-34321 IN denotes into
T16498 34322-34328 JJR denotes larger
T16499 34340-34349 NNS denotes complexes
T16500 34329-34339 JJ denotes regulatory
T16501 34349-34350 . denotes .
T16502 34350-34541 sentence denotes Tif1γ is detected within nuclear foci (see Figure 6), which, based on our analysis, do not appear to correspond to several types of previously described nuclear bodies, including PML bodies.
T16503 34351-34356 NN denotes Tif1γ
T16504 34360-34368 VBN denotes detected
T16505 34357-34359 VBZ denotes is
T16506 34369-34375 IN denotes within
T16507 34376-34383 JJ denotes nuclear
T16508 34384-34388 NNS denotes foci
T16509 34389-34390 -LRB- denotes (
T16510 34390-34393 VB denotes see
T16511 34394-34400 NN denotes Figure
T16512 34401-34402 CD denotes 6
T16513 34402-34403 -RRB- denotes )
T16514 34403-34405 , denotes ,
T16515 34405-34410 WDT denotes which
T16516 34442-34448 VB denotes appear
T16517 34410-34412 , denotes ,
T16518 34412-34417 VBN denotes based
T16519 34418-34420 IN denotes on
T16520 34421-34424 PRP$ denotes our
T16521 34425-34433 NN denotes analysis
T16522 34433-34435 , denotes ,
T16523 34435-34437 VBP denotes do
T16524 34438-34441 RB denotes not
T16525 34449-34451 TO denotes to
T16526 34452-34462 VB denotes correspond
T16527 34463-34465 IN denotes to
T16528 34466-34473 JJ denotes several
T16529 34474-34479 NNS denotes types
T16530 34480-34482 IN denotes of
T16531 34483-34493 RB denotes previously
T16532 34494-34503 VBN denotes described
T16533 34512-34518 NNS denotes bodies
T16534 34504-34511 JJ denotes nuclear
T16535 34518-34520 , denotes ,
T16536 34520-34529 VBG denotes including
T16537 34530-34533 NN denotes PML
T16538 34534-34540 NNS denotes bodies
T16539 34540-34541 . denotes .
T16540 34541-34764 sentence denotes Localization of Tif1γ to these nuclear bodies may be regulated by posttranslational modification such as SUMO modification that is required for PML to form PML nuclear domains (Zhong et al. 2000a, 2000b; Best et al. 2002).
T16541 34542-34554 NN denotes Localization
T16542 34595-34604 VBN denotes regulated
T16543 34555-34557 IN denotes of
T16544 34558-34563 NN denotes Tif1γ
T16545 34564-34566 IN denotes to
T16546 34567-34572 DT denotes these
T16547 34581-34587 NNS denotes bodies
T16548 34573-34580 JJ denotes nuclear
T16549 34588-34591 MD denotes may
T16550 34592-34594 VB denotes be
T16551 34605-34607 IN denotes by
T16552 34608-34625 JJ denotes posttranslational
T16553 34626-34638 NN denotes modification
T16554 34639-34643 JJ denotes such
T16555 34644-34646 IN denotes as
T16556 34647-34651 NN denotes SUMO
T16557 34652-34664 NN denotes modification
T16558 34665-34669 WDT denotes that
T16559 34673-34681 VBN denotes required
T16560 34670-34672 VBZ denotes is
T16561 34682-34685 IN denotes for
T16562 34693-34697 VB denotes form
T16563 34686-34689 NN denotes PML
T16564 34690-34692 TO denotes to
T16565 34698-34701 NN denotes PML
T16566 34710-34717 NNS denotes domains
T16567 34702-34709 JJ denotes nuclear
T16568 34718-34719 -LRB- denotes (
T16569 34719-34724 NNP denotes Zhong
T16570 34725-34727 FW denotes et
T16571 34728-34731 FW denotes al.
T16572 34732-34737 CD denotes 2000a
T16573 34737-34739 , denotes ,
T16574 34739-34744 CD denotes 2000b
T16575 34744-34745 : denotes ;
T16576 34746-34750 NNP denotes Best
T16577 34751-34753 FW denotes et
T16578 34754-34757 FW denotes al.
T16579 34758-34762 CD denotes 2002
T16580 34762-34763 -RRB- denotes )
T16581 34763-34764 . denotes .
T16582 34764-34994 sentence denotes These foci may serve as assembly points where Tif1γ forms multisubunit complexes with DNA-binding transcription factors and their other essential coactivators or corepressors, during the early stages of erythroid differentiation.
T16583 34765-34770 DT denotes These
T16584 34771-34775 NNS denotes foci
T16585 34780-34785 VB denotes serve
T16586 34776-34779 MD denotes may
T16587 34786-34788 IN denotes as
T16588 34789-34797 NN denotes assembly
T16589 34798-34804 NNS denotes points
T16590 34805-34810 WRB denotes where
T16591 34817-34822 VBZ denotes forms
T16592 34811-34816 NN denotes Tif1γ
T16593 34823-34835 JJ denotes multisubunit
T16594 34836-34845 NNS denotes complexes
T16595 34846-34850 IN denotes with
T16596 34851-34854 NN denotes DNA
T16597 34877-34884 NNS denotes factors
T16598 34854-34855 HYPH denotes -
T16599 34855-34862 VBG denotes binding
T16600 34863-34876 NN denotes transcription
T16601 34885-34888 CC denotes and
T16602 34889-34894 PRP$ denotes their
T16603 34911-34923 NNS denotes coactivators
T16604 34895-34900 JJ denotes other
T16605 34901-34910 JJ denotes essential
T16606 34924-34926 CC denotes or
T16607 34927-34939 NNS denotes corepressors
T16608 34939-34941 , denotes ,
T16609 34941-34947 IN denotes during
T16610 34948-34951 DT denotes the
T16611 34958-34964 NNS denotes stages
T16612 34952-34957 JJ denotes early
T16613 34965-34967 IN denotes of
T16614 34968-34977 JJ denotes erythroid
T16615 34978-34993 NN denotes differentiation
T16616 34993-34994 . denotes .
T16617 34994-35166 sentence denotes It will be important to determine the identity of Tif1γ-interacting proteins in nuclear foci and establish how they function with Tif1γ to regulate blood cell development.
T16618 34995-34997 PRP denotes It
T16619 35003-35005 VB denotes be
T16620 34998-35002 MD denotes will
T16621 35006-35015 JJ denotes important
T16622 35016-35018 TO denotes to
T16623 35019-35028 VB denotes determine
T16624 35029-35032 DT denotes the
T16625 35033-35041 NN denotes identity
T16626 35042-35044 IN denotes of
T16627 35045-35050 NN denotes Tif1γ
T16628 35051-35062 VBG denotes interacting
T16629 35050-35051 HYPH denotes -
T16630 35063-35071 NN denotes proteins
T16631 35072-35074 IN denotes in
T16632 35075-35082 JJ denotes nuclear
T16633 35083-35087 NNS denotes foci
T16634 35088-35091 CC denotes and
T16635 35092-35101 VB denotes establish
T16636 35102-35105 WRB denotes how
T16637 35111-35119 VBP denotes function
T16638 35106-35110 PRP denotes they
T16639 35120-35124 IN denotes with
T16640 35125-35130 NN denotes Tif1γ
T16641 35131-35133 TO denotes to
T16642 35134-35142 VB denotes regulate
T16643 35143-35148 NN denotes blood
T16644 35149-35153 NN denotes cell
T16645 35154-35165 NN denotes development
T16646 35165-35166 . denotes .
T17033 35191-35200 NN denotes Zebrafish
T17034 35201-35204 CC denotes and
T17035 35205-35210 NN denotes mouse
T17036 35211-35218 NNS denotes strains
T17037 35219-35222 CC denotes and
T17038 35223-35230 NNS denotes studies
T17039 35230-35300 sentence denotes Zebrafish were maintained and staged as described (Westerfield 1998).
T17040 35231-35240 NN denotes Zebrafish
T17041 35246-35256 VBN denotes maintained
T17042 35241-35245 VBD denotes were
T17043 35257-35260 CC denotes and
T17044 35261-35267 VBN denotes staged
T17045 35268-35270 IN denotes as
T17046 35271-35280 VBN denotes described
T17047 35281-35282 -LRB- denotes (
T17048 35282-35293 NNP denotes Westerfield
T17049 35294-35298 CD denotes 1998
T17050 35298-35299 -RRB- denotes )
T17051 35299-35300 . denotes .
T17052 35300-35547 sentence denotes The alleles montb222b and montg234 were generated in a large-scale screen for ENU-induced mutations (Ransom et al. 1996) on the TU, whereas the monm262 allele was derived on the AB strain and was originally called vampire (Weinstein et al. 1996).
T17053 35301-35304 DT denotes The
T17054 35305-35312 NNS denotes alleles
T17055 35341-35350 VBN denotes generated
T17056 35313-35322 NN denotes montb222b
T17057 35323-35326 CC denotes and
T17058 35327-35335 NN denotes montg234
T17059 35336-35340 VBD denotes were
T17060 35351-35353 IN denotes in
T17061 35354-35355 DT denotes a
T17062 35368-35374 NN denotes screen
T17063 35356-35361 JJ denotes large
T17064 35362-35367 NN denotes scale
T17065 35361-35362 HYPH denotes -
T17066 35375-35378 IN denotes for
T17067 35379-35382 NN denotes ENU
T17068 35383-35390 VBN denotes induced
T17069 35382-35383 HYPH denotes -
T17070 35391-35400 NNS denotes mutations
T17071 35401-35402 -LRB- denotes (
T17072 35402-35408 NNP denotes Ransom
T17073 35409-35411 FW denotes et
T17074 35412-35415 FW denotes al.
T17075 35416-35420 CD denotes 1996
T17076 35420-35421 -RRB- denotes )
T17077 35422-35424 IN denotes on
T17078 35425-35428 DT denotes the
T17079 35429-35431 NN denotes TU
T17080 35431-35433 , denotes ,
T17081 35433-35440 IN denotes whereas
T17082 35464-35471 VBN denotes derived
T17083 35441-35444 DT denotes the
T17084 35453-35459 NN denotes allele
T17085 35445-35452 NN denotes monm262
T17086 35460-35463 VBD denotes was
T17087 35472-35474 IN denotes on
T17088 35475-35478 DT denotes the
T17089 35482-35488 NN denotes strain
T17090 35479-35481 NN denotes AB
T17091 35489-35492 CC denotes and
T17092 35493-35496 VBD denotes was
T17093 35508-35514 VBN denotes called
T17094 35497-35507 RB denotes originally
T17095 35515-35522 NN denotes vampire
T17096 35523-35524 -LRB- denotes (
T17097 35524-35533 NNP denotes Weinstein
T17098 35534-35536 FW denotes et
T17099 35537-35540 FW denotes al.
T17100 35541-35545 CD denotes 1996
T17101 35545-35546 -RRB- denotes )
T17102 35546-35547 . denotes .
T17103 35547-35625 sentence denotes Mapping strains were constructed by mating to WIK or SJD polymorphic strains.
T17104 35548-35555 VBG denotes Mapping
T17105 35556-35563 NNS denotes strains
T17106 35569-35580 VBN denotes constructed
T17107 35564-35568 VBD denotes were
T17108 35581-35583 IN denotes by
T17109 35584-35590 VBG denotes mating
T17110 35591-35593 IN denotes to
T17111 35594-35597 NN denotes WIK
T17112 35617-35624 NNS denotes strains
T17113 35598-35600 CC denotes or
T17114 35601-35604 NN denotes SJD
T17115 35605-35616 JJ denotes polymorphic
T17116 35624-35625 . denotes .
T17117 35625-35726 sentence denotes Linkage analysis was performed on haploid or diploid embryos obtained from TU/SJD or TU/WIK hybrids.
T17118 35626-35633 NN denotes Linkage
T17119 35634-35642 NN denotes analysis
T17120 35647-35656 VBN denotes performed
T17121 35643-35646 VBD denotes was
T17122 35657-35659 IN denotes on
T17123 35660-35667 JJ denotes haploid
T17124 35679-35686 NNS denotes embryos
T17125 35668-35670 CC denotes or
T17126 35671-35678 JJ denotes diploid
T17127 35687-35695 VBN denotes obtained
T17128 35696-35700 IN denotes from
T17129 35701-35703 NN denotes TU
T17130 35704-35707 NN denotes SJD
T17131 35703-35704 HYPH denotes /
T17132 35718-35725 NNS denotes hybrids
T17133 35708-35710 CC denotes or
T17134 35711-35713 NN denotes TU
T17135 35714-35717 NN denotes WIK
T17136 35713-35714 HYPH denotes /
T17137 35725-35726 . denotes .
T17138 35726-35838 sentence denotes In situ hybridization and stainings of embryos were done as described (Thompson et al. 1998; Liao et al. 2002).
T17139 35727-35729 FW denotes In
T17140 35730-35734 FW denotes situ
T17141 35735-35748 NN denotes hybridization
T17142 35779-35783 VBN denotes done
T17143 35749-35752 CC denotes and
T17144 35753-35762 NNS denotes stainings
T17145 35763-35765 IN denotes of
T17146 35766-35773 NNS denotes embryos
T17147 35774-35778 VBD denotes were
T17148 35784-35786 IN denotes as
T17149 35787-35796 VBN denotes described
T17150 35797-35798 -LRB- denotes (
T17151 35798-35806 NNP denotes Thompson
T17152 35807-35809 FW denotes et
T17153 35810-35813 FW denotes al.
T17154 35814-35818 CD denotes 1998
T17155 35818-35819 : denotes ;
T17156 35820-35824 NNP denotes Liao
T17157 35825-35827 FW denotes et
T17158 35828-35831 FW denotes al.
T17159 35832-35836 CD denotes 2002
T17160 35836-35837 -RRB- denotes )
T17161 35837-35838 . denotes .
T17162 35838-35928 sentence denotes In situ hybridization of mouse embryos was performed as described (Kingsley et al. 2001).
T17163 35839-35841 FW denotes In
T17164 35842-35846 FW denotes situ
T17165 35847-35860 NN denotes hybridization
T17166 35882-35891 VBN denotes performed
T17167 35861-35863 IN denotes of
T17168 35864-35869 NN denotes mouse
T17169 35870-35877 NNS denotes embryos
T17170 35878-35881 VBD denotes was
T17171 35892-35894 IN denotes as
T17172 35895-35904 VBN denotes described
T17173 35905-35906 -LRB- denotes (
T17174 35906-35914 NNP denotes Kingsley
T17175 35915-35917 FW denotes et
T17176 35918-35921 FW denotes al.
T17177 35922-35926 CD denotes 2001
T17178 35926-35927 -RRB- denotes )
T17179 35927-35928 . denotes .
T17180 35928-36091 sentence denotes Genomic DNA isolation, genotyping, AFLP analysis, and chromosomal walking were each performed as previously described (Brownlie et al. 1998; Ransom and Zon 1999).
T17181 35929-35936 JJ denotes Genomic
T17182 35941-35950 NN denotes isolation
T17183 35937-35940 NN denotes DNA
T17184 36013-36022 VBN denotes performed
T17185 35950-35952 , denotes ,
T17186 35952-35962 NN denotes genotyping
T17187 35962-35964 , denotes ,
T17188 35964-35968 NN denotes AFLP
T17189 35969-35977 NN denotes analysis
T17190 35977-35979 , denotes ,
T17191 35979-35982 CC denotes and
T17192 35983-35994 JJ denotes chromosomal
T17193 35995-36002 NN denotes walking
T17194 36003-36007 VBD denotes were
T17195 36008-36012 RB denotes each
T17196 36023-36025 IN denotes as
T17197 36037-36046 VBN denotes described
T17198 36026-36036 RB denotes previously
T17199 36047-36048 -LRB- denotes (
T17200 36048-36056 NNP denotes Brownlie
T17201 36057-36059 FW denotes et
T17202 36060-36063 FW denotes al.
T17203 36064-36068 CD denotes 1998
T17204 36068-36069 : denotes ;
T17205 36070-36076 NNP denotes Ransom
T17206 36077-36080 CC denotes and
T17207 36081-36084 NNP denotes Zon
T17208 36085-36089 CD denotes 1999
T17209 36089-36090 -RRB- denotes )
T17210 36090-36091 . denotes .
T17211 36091-36195 sentence denotes A complete list of primers for genetic mapping, RT-PCR, and sequencing of mon are available on request.
T17212 36092-36093 DT denotes A
T17213 36103-36107 NN denotes list
T17214 36094-36102 JJ denotes complete
T17215 36170-36173 VBP denotes are
T17216 36108-36110 IN denotes of
T17217 36111-36118 NNS denotes primers
T17218 36119-36122 IN denotes for
T17219 36123-36130 JJ denotes genetic
T17220 36131-36138 NN denotes mapping
T17221 36138-36140 , denotes ,
T17222 36140-36142 NN denotes RT
T17223 36143-36146 NN denotes PCR
T17224 36142-36143 HYPH denotes -
T17225 36146-36148 , denotes ,
T17226 36148-36151 CC denotes and
T17227 36152-36162 NN denotes sequencing
T17228 36163-36165 IN denotes of
T17229 36166-36169 NN denotes mon
T17230 36174-36183 JJ denotes available
T17231 36184-36186 IN denotes on
T17232 36187-36194 NN denotes request
T17233 36194-36195 . denotes .
T17368 36374-36376 FW denotes in
T17335 36197-36201 NN denotes mRNA
T17336 36202-36212 NN denotes expression
T17337 36213-36223 NNS denotes constructs
T17338 36223-36225 , denotes ,
T17339 36225-36236 NNS denotes morpholinos
T17340 36236-36238 , denotes ,
T17341 36238-36241 CC denotes and
T17342 36242-36256 NN denotes microinjection
T17343 36256-36342 sentence denotes The full-length mon cDNA was subcloned into EcoRI and XhoI sites in the pCS2+ vector.
T17344 36257-36260 DT denotes The
T17345 36277-36281 NN denotes cDNA
T17346 36261-36265 JJ denotes full
T17347 36266-36272 NN denotes length
T17348 36265-36266 HYPH denotes -
T17349 36273-36276 NN denotes mon
T17350 36286-36295 VBN denotes subcloned
T17351 36282-36285 VBD denotes was
T17352 36296-36300 IN denotes into
T17353 36301-36306 NN denotes EcoRI
T17354 36316-36321 NNS denotes sites
T17355 36307-36310 CC denotes and
T17356 36311-36315 NN denotes XhoI
T17357 36322-36324 IN denotes in
T17358 36325-36328 DT denotes the
T17359 36335-36341 NN denotes vector
T17360 36329-36333 NN denotes pCS2
T17361 36333-36334 SYM denotes +
T17362 36341-36342 . denotes .
T17363 36342-36461 sentence denotes Synthetic mRNA was transcribed in vitro, and microinjection was performed essentially as described (Liao et al. 2002).
T17364 36343-36352 JJ denotes Synthetic
T17365 36353-36357 NN denotes mRNA
T17366 36362-36373 VBN denotes transcribed
T17367 36358-36361 VBD denotes was
T17369 36377-36382 FW denotes vitro
T17370 36382-36384 , denotes ,
T17371 36384-36387 CC denotes and
T17372 36388-36402 NN denotes microinjection
T17373 36407-36416 VBN denotes performed
T17374 36403-36406 VBD denotes was
T17375 36417-36428 RB denotes essentially
T17376 36429-36431 IN denotes as
T17377 36432-36441 VBN denotes described
T17378 36442-36443 -LRB- denotes (
T17379 36443-36447 NNP denotes Liao
T17380 36448-36450 FW denotes et
T17381 36451-36454 FW denotes al.
T17382 36455-36459 CD denotes 2002
T17383 36459-36460 -RRB- denotes )
T17384 36460-36461 . denotes .
T17642 36463-36467 NN denotes Cell
T17643 36468-36483 NN denotes transplantation
T17644 36483-36716 sentence denotes Whole kidney marrow cells were isolated from adult gata1:EGFP transgenic donors, resuspended in 0.9X phosphate-buffered saline + 5% fetal bovine serum, and injected into the sinus venosus of 2-d-old montg234 −/− and control embryos.
T17645 36484-36489 JJ denotes Whole
T17646 36504-36509 NNS denotes cells
T17647 36490-36496 NN denotes kidney
T17648 36497-36503 NN denotes marrow
T17649 36515-36523 VBN denotes isolated
T17650 36510-36514 VBD denotes were
T17651 36524-36528 IN denotes from
T17652 36529-36534 JJ denotes adult
T17653 36535-36540 NN denotes gata1
T17654 36540-36541 : denotes :
T17655 36541-36545 NN denotes EGFP
T17656 36557-36563 NNS denotes donors
T17657 36546-36556 JJ denotes transgenic
T17658 36563-36565 , denotes ,
T17659 36565-36576 VBN denotes resuspended
T17660 36577-36579 IN denotes in
T17661 36580-36583 CD denotes 0.9
T17662 36604-36610 NN denotes saline
T17663 36583-36584 SYM denotes X
T17664 36585-36594 NN denotes phosphate
T17665 36595-36603 VBN denotes buffered
T17666 36594-36595 HYPH denotes -
T17667 36611-36612 SYM denotes +
T17668 36613-36614 CD denotes 5
T17669 36614-36615 NN denotes %
T17670 36629-36634 NN denotes serum
T17671 36616-36621 JJ denotes fetal
T17672 36622-36628 JJ denotes bovine
T17673 36634-36636 , denotes ,
T17674 36636-36639 CC denotes and
T17675 36640-36648 VBN denotes injected
T17676 36649-36653 IN denotes into
T17677 36654-36657 DT denotes the
T17678 36664-36671 NN denotes venosus
T17679 36658-36663 NN denotes sinus
T17680 36672-36674 IN denotes of
T17681 36675-36676 CD denotes 2
T17682 36677-36678 NN denotes d
T17683 36676-36677 HYPH denotes -
T17684 36679-36682 JJ denotes old
T17685 36678-36679 HYPH denotes -
T17686 36708-36715 NNS denotes embryos
T17687 36683-36691 NN denotes montg234
T17688 36692-36693 SYM denotes −
T17689 36693-36694 HYPH denotes /
T17690 36694-36695 SYM denotes −
T17691 36696-36699 CC denotes and
T17692 36700-36707 NN denotes control
T17693 36715-36716 . denotes .
T17694 36716-36782 sentence denotes Between 102 and 103 kidney marrow cells were injected per embryo.
T17695 36717-36724 IN denotes Between
T17696 36725-36728 CD denotes 102
T17697 36751-36756 NNS denotes cells
T17698 36729-36732 CC denotes and
T17699 36733-36736 CD denotes 103
T17700 36737-36743 NN denotes kidney
T17701 36744-36750 NN denotes marrow
T17702 36762-36770 VBN denotes injected
T17703 36757-36761 VBD denotes were
T17704 36771-36774 IN denotes per
T17705 36775-36781 NN denotes embryo
T17706 36781-36782 . denotes .
T17707 36782-36965 sentence denotes Individual transplanted embryos were anesthetized and visualized daily under an inverted fluorescent microscope (DM-IRE2; Leica, Wetzlar, Germany) for GFP+ cells over a span of 12 d.
T17708 36783-36793 JJ denotes Individual
T17709 36807-36814 NNS denotes embryos
T17710 36794-36806 VBN denotes transplanted
T17711 36820-36832 VBN denotes anesthetized
T17712 36815-36819 VBD denotes were
T17713 36833-36836 CC denotes and
T17714 36837-36847 VBN denotes visualized
T17715 36848-36853 RB denotes daily
T17716 36854-36859 IN denotes under
T17717 36860-36862 DT denotes an
T17718 36884-36894 NN denotes microscope
T17719 36863-36871 VBN denotes inverted
T17720 36872-36883 JJ denotes fluorescent
T17721 36895-36896 -LRB- denotes (
T17722 36905-36910 NNP denotes Leica
T17723 36896-36898 NN denotes DM
T17724 36899-36903 NN denotes IRE2
T17725 36898-36899 HYPH denotes -
T17726 36903-36904 : denotes ;
T17727 36910-36912 , denotes ,
T17728 36912-36919 NNP denotes Wetzlar
T17729 36919-36921 , denotes ,
T17730 36921-36928 NNP denotes Germany
T17731 36928-36929 -RRB- denotes )
T17732 36930-36933 IN denotes for
T17733 36934-36937 NN denotes GFP
T17734 36939-36944 NNS denotes cells
T17735 36937-36938 SYM denotes +
T17736 36945-36949 IN denotes over
T17737 36950-36951 DT denotes a
T17738 36952-36956 NN denotes span
T17739 36957-36959 IN denotes of
T17740 36960-36962 CD denotes 12
T17741 36963-36964 NNS denotes d
T17742 36964-36965 . denotes .
T17743 36965-37074 sentence denotes On day 13 posttransplant, all surviving larvae (12/129; 9%) were placed in tanks and monitored for survival.
T17744 36966-36968 IN denotes On
T17745 37031-37037 VBN denotes placed
T17746 36969-36972 NN denotes day
T17747 36973-36975 CD denotes 13
T17748 36976-36990 RB denotes posttransplant
T17749 36990-36992 , denotes ,
T17750 36992-36995 DT denotes all
T17751 37006-37012 NNS denotes larvae
T17752 36996-37005 VBG denotes surviving
T17753 37013-37014 -LRB- denotes (
T17754 37017-37020 CD denotes 129
T17755 37014-37016 CD denotes 12
T17756 37016-37017 SYM denotes /
T17757 37020-37021 : denotes ;
T17758 37022-37023 CD denotes 9
T17759 37023-37024 NN denotes %
T17760 37024-37025 -RRB- denotes )
T17761 37026-37030 VBD denotes were
T17762 37038-37040 IN denotes in
T17763 37041-37046 NNS denotes tanks
T17764 37047-37050 CC denotes and
T17765 37051-37060 VBN denotes monitored
T17766 37061-37064 IN denotes for
T17767 37065-37073 NN denotes survival
T17768 37073-37074 . denotes .
T18198 37158-37163 NN denotes TIF1γ
T18199 37173-37188 NN denotes RRKRLKSDERPVHIK
T18200 37189-37192 VBD denotes was
T18201 37203-37205 IN denotes in
T18202 37206-37213 NNS denotes rabbits
T18203 37214-37215 -LRB- denotes (
T18204 37223-37232 NNP denotes Synthesis
T18182 37076-37086 NNS denotes Antibodies
T18183 37086-37088 , denotes ,
T18184 37088-37102 NN denotes immunostaining
T18185 37102-37104 , denotes ,
T18186 37104-37107 CC denotes and
T18187 37108-37119 NNS denotes immunoblots
T18188 37119-37304 sentence denotes Antisera against the human C-terminal TIF1γ sequence RRKRLKSDERPVHIK was generated in rabbits (Genemed Synthesis, South San Francisco, California, United States) and affinity purified.
T18189 37120-37128 NNS denotes Antisera
T18190 37193-37202 VBN denotes generated
T18191 37129-37136 IN denotes against
T18192 37137-37140 DT denotes the
T18193 37164-37172 NN denotes sequence
T18194 37141-37146 JJ denotes human
T18195 37147-37148 NN denotes C
T18196 37149-37157 JJ denotes terminal
T18197 37148-37149 HYPH denotes -
T18205 37215-37222 NNP denotes Genemed
T18206 37232-37234 , denotes ,
T18207 37234-37239 NNP denotes South
T18208 37244-37253 NNP denotes Francisco
T18209 37240-37243 NNP denotes San
T18210 37253-37255 , denotes ,
T18211 37255-37265 NNP denotes California
T18212 37265-37267 , denotes ,
T18213 37267-37273 NNP denotes United
T18214 37274-37280 NNP denotes States
T18215 37280-37281 -RRB- denotes )
T18216 37282-37285 CC denotes and
T18217 37286-37294 NN denotes affinity
T18218 37295-37303 VBN denotes purified
T18219 37303-37304 . denotes .
T18220 37304-37464 sentence denotes Mouse embryonic fibroblasts grown on coverslips were immunostained with HP1α (Chemicon, Temecula, California, United States) and Tif1γ antisera simultaneously.
T18221 37305-37310 NN denotes Mouse
T18222 37321-37332 NNS denotes fibroblasts
T18223 37311-37320 JJ denotes embryonic
T18224 37358-37371 VBN denotes immunostained
T18225 37333-37338 VBN denotes grown
T18226 37339-37341 IN denotes on
T18227 37342-37352 NNS denotes coverslips
T18228 37353-37357 VBD denotes were
T18229 37372-37376 IN denotes with
T18230 37377-37381 NN denotes HP1α
T18231 37440-37448 NNS denotes antisera
T18232 37382-37383 -LRB- denotes (
T18233 37383-37391 NNP denotes Chemicon
T18234 37391-37393 , denotes ,
T18235 37393-37401 NNP denotes Temecula
T18236 37401-37403 , denotes ,
T18237 37403-37413 NNP denotes California
T18238 37413-37415 , denotes ,
T18239 37415-37421 NNP denotes United
T18240 37422-37428 NNP denotes States
T18241 37428-37429 -RRB- denotes )
T18242 37430-37433 CC denotes and
T18243 37434-37439 NN denotes Tif1γ
T18244 37449-37463 RB denotes simultaneously
T18245 37463-37464 . denotes .
T18246 37464-37609 sentence denotes In brief, cells were fixed in 4% paraformaldehyde for 5 min, washed with phosphate-buffered saline, and blocked with 5% serum (PBST) for 30 min.
T18247 37465-37467 IN denotes In
T18248 37486-37491 VBN denotes fixed
T18249 37468-37473 NN denotes brief
T18250 37473-37475 , denotes ,
T18251 37475-37480 NNS denotes cells
T18252 37481-37485 VBD denotes were
T18253 37492-37494 IN denotes in
T18254 37495-37496 CD denotes 4
T18255 37496-37497 NN denotes %
T18256 37498-37514 NN denotes paraformaldehyde
T18257 37515-37518 IN denotes for
T18258 37519-37520 CD denotes 5
T18259 37521-37524 NN denotes min
T18260 37524-37526 , denotes ,
T18261 37526-37532 VBN denotes washed
T18262 37533-37537 IN denotes with
T18263 37538-37547 NN denotes phosphate
T18264 37548-37556 VBN denotes buffered
T18265 37547-37548 HYPH denotes -
T18266 37557-37563 NN denotes saline
T18267 37563-37565 , denotes ,
T18268 37565-37568 CC denotes and
T18269 37569-37576 VBN denotes blocked
T18270 37577-37581 IN denotes with
T18271 37582-37583 CD denotes 5
T18272 37583-37584 NN denotes %
T18273 37585-37590 NN denotes serum
T18274 37591-37592 -LRB- denotes (
T18275 37592-37596 NN denotes PBST
T18276 37596-37597 -RRB- denotes )
T18277 37598-37601 IN denotes for
T18278 37602-37604 CD denotes 30
T18279 37605-37608 NN denotes min
T18280 37608-37609 . denotes .
T18281 37609-37838 sentence denotes After incubation with the primary antibodies (PBST, 60 min) cells were washed three times with PBST and incubated with secondary antibodies (Jackson Laboratory, Bar Harbor, Maine, United States) followed by three washes in PBST.
T18282 37610-37615 IN denotes After
T18283 37681-37687 VBN denotes washed
T18284 37616-37626 NN denotes incubation
T18285 37627-37631 IN denotes with
T18286 37632-37635 DT denotes the
T18287 37644-37654 NNS denotes antibodies
T18288 37636-37643 JJ denotes primary
T18289 37655-37656 -LRB- denotes (
T18290 37665-37668 NN denotes min
T18291 37656-37660 NN denotes PBST
T18292 37660-37662 , denotes ,
T18293 37662-37664 CD denotes 60
T18294 37668-37669 -RRB- denotes )
T18295 37670-37675 NNS denotes cells
T18296 37676-37680 VBD denotes were
T18297 37688-37693 CD denotes three
T18298 37694-37699 NNS denotes times
T18299 37700-37704 IN denotes with
T18300 37705-37709 NN denotes PBST
T18301 37710-37713 CC denotes and
T18302 37714-37723 VBN denotes incubated
T18303 37724-37728 IN denotes with
T18304 37729-37738 JJ denotes secondary
T18305 37739-37749 NNS denotes antibodies
T18306 37750-37751 -LRB- denotes (
T18307 37759-37769 NNP denotes Laboratory
T18308 37751-37758 NNP denotes Jackson
T18309 37769-37771 , denotes ,
T18310 37771-37774 NNP denotes Bar
T18311 37775-37781 NNP denotes Harbor
T18312 37781-37783 , denotes ,
T18313 37783-37788 NNP denotes Maine
T18314 37788-37790 , denotes ,
T18315 37790-37796 NNP denotes United
T18316 37797-37803 NNP denotes States
T18317 37803-37804 -RRB- denotes )
T18318 37805-37813 VBN denotes followed
T18319 37814-37816 IN denotes by
T18320 37817-37822 CD denotes three
T18321 37823-37829 NNS denotes washes
T18322 37830-37832 IN denotes in
T18323 37833-37837 NN denotes PBST
T18324 37837-37838 . denotes .
T18325 37838-37948 sentence denotes Cells were embedded with Vectashield/DAPI and analyzed using an Axioplan 2 microscope (Zeiss, Jena, Germany).
T18326 37839-37844 NNS denotes Cells
T18327 37850-37858 VBN denotes embedded
T18328 37845-37849 VBD denotes were
T18329 37859-37863 IN denotes with
T18330 37864-37875 NN denotes Vectashield
T18331 37876-37880 NN denotes DAPI
T18332 37875-37876 HYPH denotes /
T18333 37881-37884 CC denotes and
T18334 37885-37893 VBN denotes analyzed
T18335 37894-37899 VBG denotes using
T18336 37900-37902 DT denotes an
T18337 37914-37924 NN denotes microscope
T18338 37903-37911 NN denotes Axioplan
T18339 37912-37913 CD denotes 2
T18340 37925-37926 -LRB- denotes (
T18341 37926-37931 NNP denotes Zeiss
T18342 37931-37933 , denotes ,
T18343 37933-37937 NNP denotes Jena
T18344 37937-37939 , denotes ,
T18345 37939-37946 NNP denotes Germany
T18346 37946-37947 -RRB- denotes )
T18347 37947-37948 . denotes .
T18348 37948-38066 sentence denotes Digital images were processed using the Volocity 1.0 software (Improvision, Lexington, Massachusetts, United States).
T18349 37949-37956 JJ denotes Digital
T18350 37957-37963 NNS denotes images
T18351 37969-37978 VBN denotes processed
T18352 37964-37968 VBD denotes were
T18353 37979-37984 VBG denotes using
T18354 37985-37988 DT denotes the
T18355 38002-38010 NN denotes software
T18356 37989-37997 NN denotes Volocity
T18357 37998-38001 CD denotes 1.0
T18358 38011-38012 -LRB- denotes (
T18359 38012-38023 NNP denotes Improvision
T18360 38023-38025 , denotes ,
T18361 38025-38034 NNP denotes Lexington
T18362 38034-38036 , denotes ,
T18363 38036-38049 NNP denotes Massachusetts
T18364 38049-38051 , denotes ,
T18365 38051-38057 NNP denotes United
T18366 38058-38064 NNP denotes States
T18367 38064-38065 -RRB- denotes )
T18368 38065-38066 . denotes .
T18369 38066-38164 sentence denotes G1E cell differentiation experiments were performed essentially as described (Weiss et al. 1997).
T18370 38067-38070 NN denotes G1E
T18371 38076-38091 NN denotes differentiation
T18372 38071-38075 NN denotes cell
T18373 38092-38103 NNS denotes experiments
T18374 38109-38118 VBN denotes performed
T18375 38104-38108 VBD denotes were
T18376 38119-38130 RB denotes essentially
T18377 38131-38133 IN denotes as
T18378 38134-38143 VBN denotes described
T18379 38144-38145 -LRB- denotes (
T18380 38145-38150 NNP denotes Weiss
T18381 38151-38153 FW denotes et
T18382 38154-38157 FW denotes al.
T18383 38158-38162 CD denotes 1997
T18384 38162-38163 -RRB- denotes )
T18385 38163-38164 . denotes .
T3229 4702-4705 FW denotes al.
R40 T699 T697 conj corepressors,coactivators
R7 T660 T661 det The,Gene
R8 T661 T665 nsubj Gene,Encodes
R9 T663 T661 compound Zebrafish,Gene
R10 T664 T661 compound moonshine,Gene
R11 T666 T667 amod Transcriptional,1γ
R12 T667 T665 dobj 1γ,Encodes
R13 T668 T667 amod Intermediary,1γ
R14 T669 T667 compound Factor,1γ
R15 T670 T667 punct ", ",1γ
R16 T671 T672 det an,Regulator
R17 T672 T667 appos Regulator,1γ
R18 T673 T672 amod Essential,Regulator
R19 T674 T672 prep of,Regulator
R20 T675 T674 pobj Hematopoiesis,of
R21 T679 T680 nsubjpass Hematopoiesis,orchestrated
R22 T681 T680 auxpass is,orchestrated
R23 T682 T680 advmod precisely,orchestrated
R24 T683 T680 agent by,orchestrated
R25 T684 T685 npadvmod lineage,specific
R26 T685 T687 amod specific,proteins
R27 T686 T685 punct -,specific
R28 T687 T683 pobj proteins,by
R29 T688 T689 npadvmod DNA,binding
R30 T689 T687 amod binding,proteins
R31 T690 T689 punct -,binding
R32 T691 T692 dep that,regulate
R33 T692 T687 relcl regulate,proteins
R34 T693 T692 dobj transcription,regulate
R35 T694 T692 prep in,regulate
R36 T695 T694 pobj concert,in
R37 T696 T695 prep with,concert
R38 T697 T696 pobj coactivators,with
R39 T698 T697 cc and,coactivators
R41 T700 T680 punct .,orchestrated
R42 T702 T703 nsubj Mutations,disrupt
R43 T704 T702 prep in,Mutations
R44 T705 T706 det the,gene
R45 T706 T704 pobj gene,in
R46 T707 T708 nmod zebrafish,moonshine
R47 T708 T706 nmod moonshine,gene
R48 T709 T708 punct (,moonshine
R49 T710 T708 appos mon,moonshine
R50 T711 T706 punct ),gene
R51 T712 T703 advmod specifically,disrupt
R52 T713 T714 preconj both,embryonic
R53 T714 T715 amod embryonic,hematopoiesis
R54 T715 T703 dobj hematopoiesis,disrupt
R55 T716 T714 cc and,embryonic
R56 T717 T714 conj adult,embryonic
R57 T718 T703 punct ", ",disrupt
R58 T719 T703 advcl resulting,disrupt
R59 T720 T719 prep in,resulting
R60 T721 T722 amod severe,aplasia
R61 T722 T720 pobj aplasia,in
R62 T723 T724 amod red,cell
R63 T724 T722 compound cell,aplasia
R64 T725 T724 compound blood,cell
R65 T726 T703 punct .,disrupt
R66 T728 T729 nsubj We,report
R67 T730 T731 mark that,encodes
R68 T731 T729 ccomp encodes,report
R69 T732 T731 nsubj mon,encodes
R70 T733 T734 det the,ortholog
R71 T734 T731 dobj ortholog,encodes
R72 T735 T734 compound zebrafish,ortholog
R73 T736 T734 prep of,ortholog
R74 T737 T738 amod mammalian,1γ
R75 T738 T736 pobj 1γ,of
R76 T739 T738 amod transcriptional,1γ
R77 T740 T738 amod intermediary,1γ
R78 T741 T738 compound factor,1γ
R79 T742 T738 punct (,1γ
R80 T743 T738 appos TIF1γ,1γ
R81 T744 T738 punct ),1γ
R82 T745 T738 punct (,1γ
R83 T746 T738 cc or,1γ
R84 T747 T738 conj TRIM33,1γ
R85 T748 T734 punct ),ortholog
R86 T749 T734 punct ", ",ortholog
R87 T750 T751 det a,member
R88 T751 T734 appos member,ortholog
R89 T752 T751 prep of,member
R90 T753 T754 det the,family
R91 T754 T752 pobj family,of
R92 T755 T754 compound TIF1,family
R93 T756 T754 prep of,family
R94 T757 T756 pobj coactivators,of
R95 T758 T757 cc and,coactivators
R96 T759 T757 conj corepressors,coactivators
R97 T760 T729 punct .,report
R98 T762 T763 prep During,fail
R99 T764 T762 pobj development,During
R100 T765 T763 punct ", ",fail
R101 T766 T767 amod hematopoietic,cells
R102 T767 T763 nsubj cells,fail
R103 T768 T767 compound progenitor,cells
R104 T769 T767 prep in,cells
R105 T770 T771 compound mon,mutants
R106 T771 T769 pobj mutants,in
R107 T772 T773 aux to,express
R108 T773 T763 xcomp express,fail
R109 T774 T775 amod normal,levels
R110 T775 T773 dobj levels,express
R111 T776 T775 prep of,levels
R112 T777 T778 amod hematopoietic,factors
R113 T778 T776 pobj factors,of
R114 T779 T778 compound transcription,factors
R115 T780 T775 punct ", ",levels
R116 T781 T775 prep including,levels
R117 T782 T781 pobj gata1,including
R118 T783 T763 punct ", ",fail
R119 T784 T763 cc and,fail
R120 T785 T763 conj undergo,fail
R121 T786 T785 dobj apoptosis,undergo
R122 T787 T763 punct .,fail
R123 T789 T790 nummod Three,alleles
R124 T790 T794 nsubj alleles,encode
R125 T791 T790 amod different,alleles
R126 T792 T790 nmod mon,alleles
R127 T793 T790 amod mutant,alleles
R128 T795 T790 appos each,alleles
R129 T796 T797 amod premature,codons
R130 T797 T794 dobj codons,encode
R131 T798 T797 compound stop,codons
R132 T799 T794 punct ", ",encode
R133 T800 T794 cc and,encode
R134 T801 T802 amod enforced,expression
R135 T802 T803 nsubj expression,rescues
R137 T804 T802 prep of,expression
R138 T805 T806 amod wild,type
R139 T806 T808 compound type,mRNA
R140 T807 T806 punct -,type
R141 T808 T804 pobj mRNA,of
R142 T809 T808 compound tif1γ,mRNA
R143 T810 T811 amod embryonic,hematopoiesis
R144 T811 T803 dobj hematopoiesis,rescues
R145 T812 T803 prep in,rescues
R146 T813 T814 amod homozygous,mutants
R147 T814 T812 pobj mutants,in
R148 T815 T814 compound mon,mutants
R149 T816 T803 punct .,rescues
R150 T818 T819 advmod Surprisingly,delineates
R151 T820 T819 punct ", ",delineates
R152 T821 T822 det a,level
R153 T822 T819 nsubj level,delineates
R154 T823 T822 amod high,level
R155 T824 T822 prep of,level
R156 T825 T826 amod zygotic,tif1γ
R157 T826 T827 compound tif1γ,mRNA
R158 T827 T828 compound mRNA,expression
R159 T828 T824 pobj expression,of
R160 T829 T830 amod ventral,mesoderm
R161 T830 T819 dobj mesoderm,delineates
R162 T831 T819 prep during,delineates
R163 T832 T833 amod hematopoietic,cell
R164 T833 T835 nmod cell,formation
R165 T834 T833 nmod stem,cell
R166 T835 T831 pobj formation,during
R167 T836 T833 cc and,cell
R168 T837 T833 conj progenitor,cell
R169 T838 T819 prep prior,delineates
R170 T839 T838 prep to,prior
R171 T840 T841 compound gata1,expression
R172 T841 T839 pobj expression,to
R173 T842 T819 punct .,delineates
R174 T844 T845 compound Transplantation,studies
R175 T845 T846 nsubj studies,reveal
R176 T847 T848 mark that,functions
R177 T848 T846 ccomp functions,reveal
R178 T849 T848 nsubj tif1γ,functions
R179 T850 T848 prep in,functions
R180 T851 T852 det a,manner
R181 T852 T850 pobj manner,in
R182 T853 T854 npadvmod cell,autonomous
R183 T854 T852 amod autonomous,manner
R184 T855 T854 punct -,autonomous
R185 T856 T848 prep during,functions
R186 T857 T858 det the,differentiation
R187 T858 T856 pobj differentiation,during
R188 T859 T858 prep of,differentiation
R189 T860 T861 amod erythroid,precursors
R190 T861 T859 pobj precursors,of
R191 T862 T846 punct .,reveal
R192 T864 T865 nsubj Studies,demonstrate
R193 T866 T864 prep in,Studies
R194 T867 T868 amod murine,lines
R195 T868 T866 pobj lines,in
R196 T869 T868 amod erythroid,lines
R197 T870 T868 compound cell,lines
R198 T871 T872 mark that,localized
R199 T872 T865 ccomp localized,demonstrate
R200 T873 T874 compound Tif1γ,protein
R201 T874 T872 nsubjpass protein,localized
R202 T875 T872 auxpass is,localized
R203 T876 T872 prep within,localized
R204 T877 T878 amod novel,foci
R205 T878 T876 pobj foci,within
R206 T879 T878 amod nuclear,foci
R207 T880 T872 punct ", ",localized
R208 T881 T872 cc and,localized
R209 T882 T883 nsubj expression,decreases
R210 T883 T872 conj decreases,localized
R211 T884 T883 prep during,decreases
R212 T885 T886 amod erythroid,maturation
R213 T886 T884 pobj maturation,during
R214 T887 T886 compound cell,maturation
R215 T888 T865 punct .,demonstrate
R216 T890 T891 poss Our,results
R217 T891 T892 nsubj results,establish
R218 T893 T894 det a,role
R219 T894 T892 dobj role,establish
R220 T895 T894 amod major,role
R221 T896 T894 prep for,role
R222 T897 T898 det this,factor
R223 T898 T896 pobj factor,for
R224 T899 T898 amod transcriptional,factor
R225 T900 T898 amod intermediary,factor
R226 T901 T892 prep in,establish
R227 T902 T903 det the,differentiation
R228 T903 T901 pobj differentiation,in
R229 T904 T903 prep of,differentiation
R230 T905 T906 amod hematopoietic,cells
R231 T906 T904 pobj cells,of
R232 T907 T903 prep in,differentiation
R233 T908 T907 pobj vertebrates,in
R234 T909 T892 punct .,establish
R250 T2707 T2708 nsubj Hematopoiesis,involves
R251 T2709 T2710 det the,processes
R252 T2710 T2708 dobj processes,involves
R253 T2711 T2710 amod coordinated,processes
R254 T2712 T2710 prep of,processes
R255 T2713 T2714 compound cell,proliferation
R256 T2714 T2712 pobj proliferation,of
R257 T2715 T2714 cc and,proliferation
R258 T2716 T2714 conj differentiation,proliferation
R259 T2717 T2716 prep of,differentiation
R260 T2718 T2719 det a,number
R261 T2719 T2717 pobj number,of
R262 T2720 T2721 advmod relatively,small
R263 T2721 T2719 amod small,number
R264 T2722 T2719 prep of,number
R265 T2723 T2724 compound progenitor,cells
R266 T2724 T2722 pobj cells,of
R267 T2725 T2716 prep into,differentiation
R268 T2726 T2725 pobj billions,into
R269 T2727 T2726 prep of,billions
R270 T2728 T2729 amod circulating,cells
R271 T2729 T2727 pobj cells,of
R272 T2730 T2729 amod red,cells
R273 T2731 T2730 cc and,red
R274 T2732 T2730 conj white,red
R275 T2733 T2729 compound blood,cells
R276 T2734 T2735 punct (,Thisse
R277 T2735 T2708 meta Thisse,involves
R278 T2736 T2735 cc and,Thisse
R279 T2737 T2735 conj Zon,Thisse
R280 T2738 T2737 nummod 2002,Zon
R281 T2739 T2737 punct ),Zon
R282 T2740 T2708 punct .,involves
R283 T2742 T2743 nsubj Hematopoiesis,is
R284 T2744 T2742 prep in,Hematopoiesis
R285 T2745 T2744 pobj vertebrates,in
R286 T2746 T2742 punct ", ",Hematopoiesis
R287 T2747 T2742 prep from,Hematopoiesis
R288 T2748 T2747 pobj zebrafish,from
R289 T2749 T2747 prep to,from
R290 T2750 T2749 pobj humans,to
R291 T2751 T2743 punct ", ",is
R292 T2752 T2753 det an,program
R293 T2753 T2743 attr program,is
R294 T2754 T2753 advmod evolutionarily,program
R295 T2755 T2753 amod conserved,program
R296 T2756 T2757 dep that,produces
R297 T2757 T2753 relcl produces,program
R298 T2758 T2759 nummod two,waves
R299 T2759 T2757 dobj waves,produces
R300 T2760 T2759 prep of,waves
R301 T2761 T2762 nmod stem,cells
R302 T2762 T2760 pobj cells,of
R303 T2763 T2761 cc or,stem
R304 T2764 T2761 conj progenitor,stem
R305 T2765 T2766 dep that,differ
R306 T2766 T2762 relcl differ,cells
R307 T2767 T2768 preconj both,in
R308 T2768 T2766 prep in,differ
R309 T2769 T2770 poss their,origins
R310 T2770 T2768 pobj origins,in
R311 T2771 T2770 amod embryonic,origins
R312 T2772 T2768 cc and,in
R313 T2773 T2768 conj in,in
R314 T2774 T2775 det the,lineages
R315 T2775 T2773 pobj lineages,in
R316 T2776 T2775 prep of,lineages
R317 T2777 T2778 amod differentiated,cells
R318 T2778 T2776 pobj cells,of
R319 T2779 T2778 compound blood,cells
R320 T2780 T2778 acl produced,cells
R321 T2781 T2782 punct (,Palis
R322 T2782 T2743 meta Palis,is
R323 T2783 T2782 cc and,Palis
R324 T2784 T2782 conj Yoder,Palis
R325 T2785 T2784 nummod 2001,Yoder
R326 T2786 T2784 punct ;,Yoder
R327 T2787 T2784 conj Orkin,Yoder
R328 T2788 T2787 cc and,Orkin
R329 T2789 T2787 conj Zon,Orkin
R330 T2790 T2789 nummod 2002,Zon
R331 T2791 T2789 punct ;,Zon
R332 T2792 T2789 conj Galloway,Zon
R333 T2793 T2792 cc and,Galloway
R334 T2794 T2792 conj Zon,Galloway
R335 T2795 T2794 nummod 2003,Zon
R336 T2796 T2794 punct ),Zon
R337 T2797 T2743 punct .,is
R338 T2799 T2800 det The,wave
R339 T2800 T2806 nsubj wave,originates
R340 T2801 T2800 amod first,wave
R341 T2802 T2801 punct ", ",first
R342 T2803 T2801 cc or,first
R343 T2804 T2801 conj primitive,first
R344 T2805 T2800 punct ", ",wave
R345 T2807 T2800 prep of,wave
R346 T2808 T2807 pobj hematopoiesis,of
R347 T2809 T2806 prep from,originates
R348 T2810 T2811 amod ventral,mesoderm
R349 T2811 T2809 pobj mesoderm,from
R350 T2812 T2806 cc and,originates
R351 T2813 T2806 conj gives,originates
R352 T2814 T2813 dobj rise,gives
R353 T2815 T2813 prep to,gives
R354 T2816 T2817 compound progenitor,cells
R355 T2817 T2815 pobj cells,to
R356 T2818 T2819 dep that,differentiate
R357 T2819 T2817 relcl differentiate,cells
R358 T2820 T2819 prep in,differentiate
R359 T2821 T2822 amod embryonic,islands
R360 T2822 T2820 pobj islands,in
R361 T2823 T2822 compound blood,islands
R362 T2824 T2806 punct .,originates
R363 T2826 T2827 det The,wave
R364 T2827 T2829 nsubj wave,produces
R365 T2828 T2827 amod primitive,wave
R366 T2830 T2827 prep of,wave
R367 T2831 T2830 pobj hematopoiesis,of
R368 T2832 T2833 det a,burst
R369 T2833 T2829 dobj burst,produces
R370 T2834 T2833 prep of,burst
R371 T2835 T2836 amod embryonic,erythrocytes
R372 T2836 T2834 pobj erythrocytes,of
R373 T2837 T2836 cc and,erythrocytes
R374 T2838 T2836 conj macrophages,erythrocytes
R375 T2839 T2829 punct .,produces
R376 T2841 T2842 det The,wave
R377 T2842 T2848 nsubj wave,arises
R378 T2843 T2842 amod second,wave
R379 T2844 T2843 punct ", ",second
R380 T2845 T2843 cc or,second
R381 T2846 T2843 conj definitive,second
R382 T2847 T2842 punct ", ",wave
R383 T2849 T2842 prep of,wave
R384 T2850 T2849 pobj hematopoiesis,of
R385 T2851 T2848 prep from,arises
R386 T2852 T2853 npadvmod self,renewing
R387 T2853 T2855 amod renewing,cells
R388 T2911 T2910 dobj genes,characterize
R389 T2854 T2853 punct -,renewing
R390 T2912 T2913 dep that,control
R391 T2913 T2911 relcl control,genes
R392 T2914 T2913 dobj hematopoiesis,control
R393 T2855 T2851 pobj cells,from
R394 T2915 T2904 advcl using,undertaken
R395 T2916 T2917 det the,zebrafish
R396 T2917 T2915 dobj zebrafish,using
R397 T2856 T2855 compound stem,cells
R398 T2918 T2915 prep as,using
R399 T2919 T2920 det a,system
R400 T2920 T2918 pobj system,as
R401 T2857 T2858 dep that,develop
R402 T2921 T2920 compound model,system
R403 T2922 T2923 punct (,Thisse
R404 T2858 T2855 relcl develop,cells
R405 T2923 T2904 meta Thisse,undertaken
R406 T2924 T2923 cc and,Thisse
R407 T2925 T2923 conj Zon,Thisse
R408 T2859 T2858 advmod primarily,develop
R409 T2926 T2925 nummod 2002,Zon
R410 T2927 T2925 punct ),Zon
R411 T2928 T2904 punct .,undertaken
R412 T2860 T2858 prep in,develop
R413 T2930 T2931 prep As,identified
R414 T2861 T2862 det the,region
R415 T2932 T2930 pobj part,As
R416 T2933 T2932 prep of,part
R417 T2862 T2860 pobj region,in
R418 T2863 T2862 amod intraembryonic,region
R419 T2934 T2935 det a,screen
R420 T2864 T2865 compound aorta,mesonephros
R421 T2935 T2933 pobj screen,of
R422 T2865 T2862 compound mesonephros,region
R423 T2936 T2937 amod large,scale
R424 T2937 T2935 nmod scale,screen
R425 T2938 T2937 punct -,scale
R426 T2866 T2865 punct –,mesonephros
R427 T2939 T2935 amod forward,screen
R428 T2940 T2935 amod genetic,screen
R429 T2941 T2931 punct ", ",identified
R430 T2867 T2865 compound gonad,mesonephros
R431 T2942 T2931 nsubj we,identified
R432 T2868 T2865 punct –,mesonephros
R433 T2943 T2931 advmod previously,identified
R434 T2944 T2945 amod bloodless,mutants
R435 T2945 T2931 dobj mutants,identified
R436 T2946 T2945 compound zebrafish,mutants
R437 T2869 T2848 punct .,arises
R438 T2947 T2948 dep that,failed
R439 T2948 T2945 relcl failed,mutants
R440 T2949 T2950 aux to,express
R441 T2871 T2872 det These,cells
R442 T2950 T2948 xcomp express,failed
R443 T2951 T2952 det the,gata1
R444 T2952 T2950 dobj gata1,express
R445 T2953 T2952 amod erythroid,gata1
R446 T2954 T2952 compound transcription,gata1
R447 T2955 T2952 compound factor,gata1
R448 T2872 T2876 nsubj cells,seed
R449 T2956 T2950 advmod normally,express
R450 T2957 T2950 prep in,express
R451 T2873 T2872 amod definitive,cells
R452 T2958 T2959 amod embryonic,precursors
R453 T2959 T2957 pobj precursors,in
R454 T2874 T2872 amod hematopoietic,cells
R455 T2960 T2959 amod hematopoietic,precursors
R456 T2961 T2962 punct (,Ransom
R457 T2962 T2931 meta Ransom,identified
R458 T2875 T2872 compound stem,cells
R459 T2963 T2962 nmod et,Ransom
R460 T2964 T2962 nmod al.,Ransom
R461 T2877 T2878 det the,spaces
R462 T2965 T2962 nummod 1996,Ransom
R463 T2966 T2962 punct ),Ransom
R464 T2967 T2931 punct .,identified
R465 T2878 T2876 dobj spaces,seed
R466 T2969 T2970 nsubj We,named
R467 T2879 T2878 advmod later,spaces
R468 T2971 T2970 dobj one,named
R469 T2972 T2971 prep of,one
R470 T2973 T2974 det these,genes
R471 T2880 T2878 amod developing,spaces
R472 T2974 T2972 pobj genes,of
R473 T2975 T2974 compound zebrafish,genes
R474 T2881 T2878 compound marrow,spaces
R475 T2976 T2970 oprd moonshine,named
R476 T2977 T2976 punct (,moonshine
R477 T2978 T2976 appos mon,moonshine
R478 T2882 T2876 punct ", ",seed
R479 T2979 T2970 punct ),named
R480 T2980 T2970 punct ", ",named
R481 T2981 T2970 cc and,named
R482 T2883 T2884 aux to,produce
R483 T2982 T2983 det another,group
R484 T2983 T2984 nsubj group,named
R485 T2884 T2876 advcl produce,seed
R486 T2984 T2970 conj named,named
R487 T2985 T2986 det a,allele
R488 T2885 T2886 det all,lineages
R489 T2986 T2984 dobj allele,named
R490 T2987 T2986 amod noncomplementing,allele
R491 T2886 T2884 dobj lineages,produce
R492 T2988 T2984 oprd vampire,named
R493 T2989 T2990 punct (,Weinstein
R494 T2990 T2984 meta Weinstein,named
R495 T2991 T2990 nmod et,Weinstein
R496 T2992 T2990 nmod al.,Weinstein
R497 T2993 T2990 nummod 1996,Weinstein
R498 T2887 T2886 prep of,lineages
R501 T2888 T2889 amod adult,cells
R502 T2997 T2998 advmod Here,determined
R503 T2999 T2998 punct ", ",determined
R504 T2889 T2887 pobj cells,of
R505 T3000 T2998 nsubj we,determined
R506 T3001 T2998 aux have,determined
R507 T3002 T3003 mark that,cause
R508 T2890 T2889 compound blood,cells
R509 T3003 T2998 ccomp cause,determined
R510 T3004 T3003 nsubj mutations,cause
R511 T2891 T2886 punct ", ",lineages
R512 T3005 T3004 prep in,mutations
R513 T3006 T3007 det the,gene
R514 T3007 T3005 pobj gene,in
R515 T2892 T2886 prep including,lineages
R516 T3008 T3007 compound mon,gene
R517 T3009 T3010 det a,disruption
R518 T3010 T3003 dobj disruption,cause
R519 T2893 T2894 amod definitive,erythrocytes
R520 T3011 T3010 prep in,disruption
R521 T3012 T3013 preconj both,embryonic
R522 T2894 T2892 pobj erythrocytes,including
R523 T3013 T3015 amod embryonic,hematopoiesis
R524 T3014 T3013 amod primitive,embryonic
R525 T3015 T3011 pobj hematopoiesis,in
R526 T2895 T2894 punct ", ",erythrocytes
R527 T3016 T3013 cc and,embryonic
R528 T2896 T2897 amod myeloid,cells
R529 T2897 T2894 conj cells,erythrocytes
R530 T2898 T2897 punct ", ",cells
R531 T3017 T3018 amod definitive,adult
R532 T3018 T3013 conj adult,embryonic
R533 T2899 T2897 cc and,cells
R534 T3019 T3003 punct ", ",cause
R535 T3020 T3003 advcl resulting,cause
R536 T2900 T2897 conj lymphocytes,cells
R537 T3021 T3020 prep in,resulting
R538 T3022 T3023 det a,loss
R539 T2901 T2876 punct .,seed
R540 T3023 T3021 pobj loss,in
R541 T3024 T3023 amod severe,loss
R542 T3025 T3023 prep of,loss
R543 T2903 T2904 nsubj We,undertaken
R544 T3026 T3027 amod erythroid,cells
R545 T3027 T3025 pobj cells,of
R546 T3028 T2998 punct .,determined
R547 T3030 T3031 amod Erythroid,cells
R548 T3031 T3033 nsubj cells,are
R549 T2905 T2904 aux have,undertaken
R550 T3032 T3031 compound progenitor,cells
R551 T3034 T3031 prep in,cells
R552 T3035 T3036 compound mon,mutants
R553 T2906 T2907 det a,approach
R554 T3036 T3034 pobj mutants,in
R555 T3037 T3033 advmod initially,are
R556 T3038 T3033 acomp present,are
R557 T2907 T2904 dobj approach,undertaken
R558 T3039 T3033 punct ", ",are
R559 T3040 T3033 cc but,are
R560 T3041 T3033 conj fail,are
R561 T2908 T2907 amod genetic,approach
R562 T3042 T3043 aux to,express
R563 T2909 T2910 aux to,characterize
R564 T3043 T3041 xcomp express,fail
R565 T2910 T2904 advcl characterize,undertaken
R566 T3044 T3045 amod normal,levels
R567 T3045 T3043 dobj levels,express
R568 T3046 T3045 prep of,levels
R569 T3123 T3121 compound TIF1,family
R570 T3047 T3048 amod hematopoietic,factors
R571 T3048 T3046 pobj factors,of
R572 T3124 T3116 punct (,members
R573 T3049 T3048 compound transcription,factors
R574 T3050 T3043 cc and,express
R575 T3051 T3043 conj undergo,express
R576 T3125 T3116 appos α,members
R577 T3052 T3051 dobj apoptosis,undergo
R578 T3053 T3033 punct .,are
R579 T3126 T3125 punct ", ",α
R580 T3055 T3056 amod Positional,cloning
R581 T3127 T3125 conj β,α
R582 T3056 T3057 nsubj cloning,identifies
R583 T3058 T3059 det the,gene
R584 T3128 T3127 punct ", ",β
R585 T3059 T3057 dobj gene,identifies
R586 T3060 T3059 compound mon,gene
R587 T3061 T3057 prep as,identifies
R588 T3129 T3127 cc and,β
R589 T3062 T3063 det the,ortholog
R590 T3063 T3061 pobj ortholog,as
R591 T3064 T3063 compound zebrafish,ortholog
R592 T3065 T3063 prep of,ortholog
R593 T3130 T3127 punct γ,β
R594 T3066 T3067 amod mammalian,1γ
R595 T3067 T3065 pobj 1γ,of
R596 T3131 T3118 punct ),are
R597 T3068 T3067 amod transcriptional,1γ
R598 T3069 T3067 amod intermediary,1γ
R599 T3070 T3067 compound factor,1γ
R600 T3071 T3067 punct (,1γ
R601 T3072 T3067 appos TIF1γ,1γ
R602 T3073 T3067 punct ),1γ
R603 T3132 T3133 amod large,proteins
R604 T3074 T3067 punct ", ",1γ
R605 T3075 T3076 det a,member
R606 T3076 T3067 appos member,1γ
R607 T3133 T3118 attr proteins,are
R608 T3077 T3076 prep of,member
R609 T3078 T3079 det the,family
R610 T3079 T3077 pobj family,of
R611 T3134 T3133 amod nuclear,proteins
R612 T3080 T3079 compound TIF1,family
R613 T3081 T3079 prep of,family
R614 T3082 T3083 amod transcriptional,coactivators
R615 T3083 T3081 pobj coactivators,of
R616 T3135 T3136 dep that,contain
R617 T3084 T3083 cc and,coactivators
R618 T3085 T3083 conj corepressors,coactivators
R619 T3136 T3133 relcl contain,proteins
R620 T3086 T3087 punct (,Le
R621 T3087 T3057 meta Le,identifies
R622 T3088 T3087 nmod Douarin,Le
R623 T3137 T3135 nmod each,that
R624 T3089 T3087 nmod et,Le
R625 T3090 T3087 nmod al.,Le
R626 T3091 T3087 nummod 1995,Le
R627 T3138 T3139 det an,RBCC
R628 T3092 T3087 punct ;,Le
R629 T3093 T3087 nmod Friedman,Le
R630 T3094 T3087 nmod et,Le
R631 T3139 T3136 dobj RBCC,contain
R632 T3095 T3087 nmod al.,Le
R633 T3096 T3087 nummod 1996,Le
R634 T3097 T3087 punct ;,Le
R635 T3140 T3141 npadvmod N,terminal
R636 T3098 T3087 nmod Kim,Le
R637 T3099 T3087 nmod et,Le
R638 T3100 T3087 nmod al.,Le
R639 T3141 T3139 amod terminal,RBCC
R640 T3101 T3087 nummod 1996,Le
R641 T3102 T3087 punct ;,Le
R642 T3142 T3141 punct -,terminal
R643 T3103 T3087 nmod Venturini,Le
R644 T3104 T3087 nmod et,Le
R645 T3105 T3087 nmod al.,Le
R646 T3106 T3087 nummod 1999,Le
R647 T3143 T3139 cc or,RBCC
R648 T3107 T3087 punct ;,Le
R649 T3108 T3087 nmod Peng,Le
R650 T3144 T3145 compound TRIM,domain
R651 T3109 T3087 nmod et,Le
R652 T3110 T3087 nmod al.,Le
R653 T3111 T3087 nummod 2002,Le
R654 T3112 T3087 punct ),Le
R655 T3113 T3057 punct .,identifies
R656 T3145 T3139 conj domain,RBCC
R657 T3115 T3116 det The,members
R658 T3116 T3118 nsubj members,are
R659 T3117 T3116 nummod three,members
R660 T3146 T3147 punct (,Reymond
R661 T3119 T3116 prep of,members
R662 T3120 T3121 det the,family
R663 T3147 T3145 meta Reymond,domain
R664 T3121 T3119 pobj family,of
R665 T3122 T3121 compound vertebrate,family
R666 T3148 T3147 nmod et,Reymond
R667 T3149 T3147 nmod al.,Reymond
R668 T3150 T3147 nummod 2001,Reymond
R669 T3229 T3227 nmod al.,Zhong
R670 T3151 T3147 punct ),Reymond
R671 T3230 T3227 nummod 1999,Zhong
R672 T3231 T3227 punct ),Zhong
R673 T3152 T3139 acl composed,RBCC
R674 T3232 T3195 punct .,shown
R675 T3153 T3152 prep of,composed
R676 T3234 T3235 nsubjpass TIF1β,shown
R677 T3236 T3235 aux has,shown
R678 T3154 T3155 det a,finger
R679 T3237 T3235 auxpass been,shown
R680 T3238 T3239 aux to,act
R681 T3155 T3153 pobj finger,of
R682 T3239 T3235 xcomp act,shown
R683 T3240 T3239 prep as,act
R684 T3241 T3242 det a,corepressor
R685 T3156 T3155 compound RING,finger
R686 T3242 T3240 pobj corepressor,as
R687 T3243 T3242 prep for,corepressor
R688 T3157 T3155 punct ", ",finger
R689 T3244 T3245 det the,family
R690 T3245 T3243 pobj family,for
R691 T3246 T3245 amod large,family
R692 T3247 T3245 prep of,family
R693 T3158 T3159 nummod two,boxes
R694 T3248 T3249 npadvmod Krüppel,associated
R695 T3159 T3155 conj boxes,finger
R696 T3249 T3251 amod associated,box
R697 T3250 T3249 punct -,associated
R698 T3160 T3159 compound B,boxes
R699 T3251 T3252 nmod box,domain
R700 T3252 T3256 compound domain,factors
R701 T3161 T3159 punct -,boxes
R702 T3253 T3251 punct (,box
R703 T3254 T3251 appos KRAB,box
R704 T3255 T3252 punct ),domain
R705 T3256 T3247 pobj factors,of
R706 T3257 T3258 compound zinc,finger
R707 T3162 T3159 punct ", ",boxes
R708 T3258 T3256 compound finger,factors
R709 T3259 T3258 punct -,finger
R710 T3260 T3256 compound transcription,factors
R711 T3163 T3159 cc and,boxes
R712 T3261 T3262 punct (,Friedman
R713 T3262 T3235 meta Friedman,shown
R714 T3164 T3165 det a,domain
R715 T3263 T3262 nmod et,Friedman
R716 T3264 T3262 nmod al.,Friedman
R717 T3265 T3262 nummod 1996,Friedman
R718 T3165 T3159 conj domain,boxes
R719 T3266 T3262 punct ;,Friedman
R720 T3267 T3262 nmod Abrink,Friedman
R721 T3166 T3167 amod coiled,coil
R722 T3268 T3262 nmod et,Friedman
R723 T3269 T3262 nmod al.,Friedman
R724 T3270 T3262 nummod 2001,Friedman
R725 T3271 T3262 punct ),Friedman
R726 T3167 T3165 compound coil,domain
R727 T3272 T3235 punct .,shown
R728 T3168 T3167 punct -,coil
R729 T3274 T3275 prep In,associate
R730 T3169 T3118 punct .,are
R731 T3276 T3274 pobj contrast,In
R732 T3277 T3275 punct ", ",associate
R733 T3278 T3275 nsubj TIF1γ,associate
R734 T3171 T3172 compound TIF1,members
R735 T3279 T3275 aux does,associate
R736 T3280 T3275 neg not,associate
R737 T3281 T3275 advmod directly,associate
R738 T3282 T3275 prep with,associate
R739 T3283 T3284 preconj either,receptors
R740 T3172 T3174 nsubj members,contain
R741 T3284 T3282 pobj receptors,with
R742 T3285 T3284 amod nuclear,receptors
R743 T3173 T3172 compound family,members
R744 T3286 T3284 cc or,receptors
R745 T3287 T3288 compound KRAB,domains
R746 T3288 T3284 conj domains,receptors
R747 T3175 T3174 advmod also,contain
R748 T3289 T3290 dep that,bind
R749 T3290 T3284 relcl bind,receptors
R750 T3291 T3290 prep to,bind
R751 T3292 T3293 det the,members
R752 T3176 T3177 det a,finger
R753 T3293 T3291 pobj members,to
R754 T3294 T3293 amod other,members
R755 T3295 T3293 compound TIF1,members
R756 T3296 T3293 compound family,members
R757 T3297 T3298 punct (,Venturini
R758 T3177 T3174 dobj finger,contain
R759 T3298 T3275 meta Venturini,associate
R760 T3178 T3177 nmod C,finger
R761 T3299 T3298 nmod et,Venturini
R762 T3300 T3298 nmod al.,Venturini
R763 T3179 T3178 punct -,C
R764 T3301 T3298 nummod 1999,Venturini
R765 T3302 T3298 punct ;,Venturini
R766 T3303 T3298 nmod Abrink,Venturini
R767 T3180 T3178 amod terminal,C
R768 T3304 T3298 nmod et,Venturini
R769 T3305 T3298 nmod al.,Venturini
R770 T3181 T3177 compound plant,finger
R771 T3306 T3298 nummod 2001,Venturini
R772 T3307 T3298 punct ),Venturini
R773 T3182 T3177 compound homeodomain,finger
R774 T3308 T3275 punct .,associate
R775 T3310 T3311 amod Biochemical,studies
R776 T3183 T3177 cc and,finger
R777 T3311 T3312 nsubj studies,demonstrate
R778 T3184 T3177 conj bromodomain,finger
R779 T3313 T3312 advmod also,demonstrate
R780 T3314 T3315 mark that,forms
R781 T3315 T3312 ccomp forms,demonstrate
R782 T3185 T3186 dep that,are
R783 T3316 T3315 nsubj TIF1γ,forms
R784 T3317 T3318 preconj both,homo-oligomers
R785 T3186 T3177 relcl are,finger
R786 T3318 T3315 dobj homo-oligomers,forms
R787 T3319 T3318 cc and,homo-oligomers
R788 T3187 T3186 acomp characteristic,are
R789 T3188 T3187 prep of,characteristic
R790 T3320 T3318 conj hetero-oligomers,homo-oligomers
R791 T3321 T3318 prep with,homo-oligomers
R792 T3189 T3190 npadvmod chromatin,remodeling
R793 T3322 T3321 pobj TIF1α,with
R794 T3323 T3321 cc but,with
R795 T3190 T3191 amod remodeling,factors
R796 T3324 T3323 neg not,but
R797 T3325 T3321 conj with,with
R798 T3191 T3188 pobj factors,of
R799 T3326 T3325 pobj TIF1β,with
R800 T3327 T3328 punct (,Peng
R801 T3192 T3174 punct .,contain
R802 T3328 T3312 meta Peng,demonstrate
R803 T3329 T3328 nmod et,Peng
R804 T3330 T3328 nmod al.,Peng
R805 T3194 T3195 nsubjpass TIF1α,shown
R806 T3331 T3328 nummod 2002,Peng
R807 T3332 T3328 punct ),Peng
R808 T3333 T3312 punct .,demonstrate
R809 T3196 T3195 aux has,shown
R810 T3197 T3195 auxpass been,shown
R811 T3335 T3336 det The,genes
R812 T3336 T3341 nsubjpass genes,subjected
R813 T3337 T3336 amod murine,genes
R814 T3198 T3199 aux to,associate
R815 T3338 T3336 nmod Tif1α,genes
R816 T3339 T3338 cc and,Tif1α
R817 T3199 T3195 xcomp associate,shown
R818 T3340 T3338 conj Tif1γ,Tif1α
R819 T3200 T3199 prep with,associate
R820 T3342 T3341 aux have,subjected
R821 T3343 T3341 neg not,subjected
R822 T3344 T3341 advmod yet,subjected
R823 T3201 T3202 det a,variety
R824 T3345 T3341 auxpass been,subjected
R825 T3346 T3341 prep to,subjected
R826 T3202 T3200 pobj variety,with
R827 T3347 T3348 npadvmod gene,targeting
R828 T3348 T3349 amod targeting,experiments
R829 T3349 T3346 pobj experiments,to
R830 T3203 T3202 prep of,variety
R831 T3350 T3341 punct ", ",subjected
R832 T3351 T3352 mark whereas,demonstrates
R833 T3204 T3205 nmod ligand,receptors
R834 T3352 T3341 advcl demonstrates,subjected
R835 T3353 T3352 nsubj analysis,demonstrates
R836 T3354 T3353 prep of,analysis
R837 T3205 T3203 pobj receptors,of
R838 T3355 T3356 compound mouse,mutants
R839 T3356 T3354 pobj mutants,of
R840 T3357 T3358 mark that,required
R841 T3206 T3204 punct -,ligand
R842 T3358 T3352 ccomp required,demonstrates
R845 T3207 T3204 amod bound,ligand
R848 T3208 T3205 amod nuclear,receptors
R849 T3363 T3361 pobj embryogenesis,for
R850 T3364 T3363 cc and,embryogenesis
R851 T3365 T3366 compound mesoderm,induction
R852 T3209 T3205 compound hormone,receptors
R853 T3366 T3363 conj induction,embryogenesis
R854 T3367 T3363 prep in,embryogenesis
R855 T3210 T3211 punct (,Le
R856 T3368 T3367 amod particular,in
R857 T3369 T3370 punct (,Cammas
R858 T3370 T3341 meta Cammas,subjected
R859 T3211 T3199 meta Le,associate
R860 T3371 T3370 nmod et,Cammas
R861 T3372 T3370 nmod al.,Cammas
R862 T3373 T3370 nummod 2000,Cammas
R863 T3374 T3370 punct ),Cammas
R864 T3375 T3341 punct .,subjected
R865 T3212 T3211 nmod Douarin,Le
R866 T3377 T3378 advcl Taken,suggest
R867 T3213 T3211 nmod et,Le
R868 T3379 T3377 advmod together,Taken
R869 T3380 T3378 punct ", ",suggest
R870 T3381 T3382 det these,studies
R871 T3214 T3211 nmod al.,Le
R872 T3382 T3378 nsubj studies,suggest
R873 T3383 T3384 mark that,is
R874 T3215 T3211 nummod 1995,Le
R875 T3216 T3211 punct ),Le
R876 T3384 T3378 ccomp is,suggest
R877 T3385 T3386 det a,function
R878 T3217 T3199 cc and,associate
R879 T3386 T3384 nsubj function,is
R880 T3387 T3386 amod major,function
R881 T3388 T3386 prep of,function
R882 T3218 T3199 conj function,associate
R883 T3389 T3390 compound TIF1,members
R884 T3390 T3388 pobj members,of
R885 T3391 T3390 compound family,members
R886 T3219 T3218 prep as,function
R887 T3392 T3393 aux to,link
R888 T3393 T3384 xcomp link,is
R889 T3220 T3221 det a,coactivator
R890 T3394 T3395 nmod DNA,proteins
R891 T3395 T3393 dobj proteins,link
R892 T3221 T3219 pobj coactivator,as
R893 T3396 T3394 punct -,DNA
R894 T3397 T3394 amod binding,DNA
R895 T3222 T3221 prep for,coactivator
R896 T3398 T3393 prep with,link
R897 T3399 T3400 amod other,coactivators
R898 T3400 T3398 pobj coactivators,with
R899 T3223 T3224 amod retinoic,acid
R900 T3401 T3400 cc or,coactivators
R901 T3402 T3400 conj corepressors,coactivators
R902 T3403 T3393 prep during,link
R903 T3224 T3225 compound acid,receptors
R904 T3404 T3403 pobj development,during
R905 T3405 T3378 punct .,suggest
R906 T3225 T3222 pobj receptors,for
R907 T3407 T3408 poss Our,studies
R908 T3408 T3409 nsubj studies,establish
R909 T3226 T3227 punct (,Zhong
R910 T3410 T3411 mark that,functions
R911 T3227 T3218 meta Zhong,function
R912 T3411 T3409 ccomp functions,establish
R913 T3412 T3411 nsubj tif1γ,functions
R914 T3413 T3411 prep as,functions
R915 T3414 T3415 det an,regulator
R916 T3415 T3413 pobj regulator,as
R917 T3416 T3415 amod essential,regulator
R918 T3228 T3227 nmod et,Zhong
R919 T3417 T3415 prep of,regulator
R920 T3418 T3419 amod embryonic,hematopoiesis
R921 T3419 T3417 pobj hematopoiesis,of
R922 T3440 T3441 amod embryonic,hematopoiesis
R923 T3420 T3418 cc and,embryonic
R924 T3421 T3418 conj adult,embryonic
R925 T3422 T3411 prep in,functions
R926 T3423 T3422 pobj vertebrates,in
R927 T3424 T3409 punct .,establish
R928 T3441 T3439 pobj hematopoiesis,during
R929 T3426 T3427 compound Cell,studies
R930 T3442 T3429 punct .,demonstrate
R931 T3427 T3429 nsubj studies,demonstrate
R932 T3428 T3427 compound transplantation,studies
R933 T3444 T3445 det The,gene
R934 T3430 T3431 mark that,acts
R935 T3431 T3429 ccomp acts,demonstrate
R936 T3432 T3431 nsubj tif1γ,acts
R937 T3433 T3431 prep in,acts
R938 T3434 T3435 det a,manner
R939 T3445 T3447 nsubjpass gene,expressed
R940 T3435 T3433 pobj manner,in
R941 T3436 T3435 nmod cell,manner
R942 T3437 T3436 punct -,cell
R943 T3446 T3445 compound tif1γ,gene
R944 T3438 T3436 amod autonomous,cell
R945 T3439 T3431 prep during,acts
R946 T3448 T3447 auxpass is,expressed
R947 T3449 T3447 advmod specifically,expressed
R948 T3450 T3447 prep in,expressed
R949 T3451 T3452 amod ventral,mesoderm
R950 T3452 T3453 nmod mesoderm,progenitors
R951 T3453 T3450 pobj progenitors,in
R952 T3454 T3452 cc and,mesoderm
R953 T3455 T3452 conj hematopoietic,mesoderm
R954 T3456 T3447 punct ", ",expressed
R955 T3457 T3458 advmod then,downregulated
R956 T3458 T3447 dep downregulated,expressed
R957 T3459 T3460 mark as,occurs
R958 T3460 T3458 advcl occurs,downregulated
R959 T3461 T3462 amod erythroid,maturation
R960 T3462 T3460 nsubj maturation,occurs
R961 T3463 T3447 punct .,expressed
R962 T3465 T3466 compound Tif1γ,protein
R963 T3466 T3467 nsubj protein,localizes
R964 T3468 T3467 prep to,localizes
R965 T3469 T3470 det a,class
R966 T3470 T3468 pobj class,to
R967 T3471 T3470 amod novel,class
R968 T3472 T3470 prep of,class
R969 T3473 T3474 amod nuclear,bodies
R970 T3474 T3472 pobj bodies,of
R971 T3475 T3467 prep in,localizes
R972 T3476 T3477 preconj both,fibroblasts
R973 T3477 T3475 pobj fibroblasts,in
R974 T3478 T3477 amod primary,fibroblasts
R975 T3479 T3480 compound mouse,embryo
R976 T3480 T3477 compound embryo,fibroblasts
R977 T3481 T3477 cc and,fibroblasts
R978 T3482 T3483 compound erythroleukemia,lines
R979 T3483 T3477 conj lines,fibroblasts
R980 T3484 T3483 compound cell,lines
R981 T3485 T3467 punct .,localizes
R982 T3487 T3488 advcl Taken,demonstrate
R983 T3489 T3487 advmod together,Taken
R984 T3490 T3488 punct ", ",demonstrate
R985 T3491 T3492 poss our,studies
R986 T3492 T3488 nsubj studies,demonstrate
R987 T3493 T3494 mark that,required
R988 T3494 T3488 ccomp required,demonstrate
R989 T3495 T3494 nsubjpass Tif1γ,required
R990 T3496 T3494 auxpass is,required
R991 T3497 T3494 prep for,required
R992 T3498 T3499 amod normal,development
R993 T3499 T3497 pobj development,for
R994 T3500 T3499 amod erythroid,development
R995 T3501 T3499 compound cell,development
R996 T3502 T3499 cc and,development
R997 T3503 T3499 conj survival,development
R998 T3504 T3488 punct .,demonstrate
R1013 T4640 T4641 det The,Gene
R1014 T4641 T4644 nsubj Gene,Is
R1015 T4642 T4641 compound Zebrafish,Gene
R1016 T4643 T4641 compound "moonshine Gene Encodes Transcriptional Intermediary Factor 1γ, an Essential Regulator of Hematopoiesis moonshine/tif1γ in Hematopoiesis Abstract Hematopoiesis is precisely orchestrated by lineage-specific DNA-binding proteins that regulate transcription in concert with coactivators and corepressors. Mutations in the zebrafish moonshine (mon) gene specifically disrupt both embryonic and adult hematopoiesis, resulting in severe red blood cell aplasia. We report that mon encodes the zebrafish ortholog of mammalian transcriptional intermediary factor 1γ (TIF1γ) (or TRIM33), a member of the TIF1 family of coactivators and corepressors. During development, hematopoietic progenitor cells in mon mutants fail to express normal levels of hematopoietic transcription factors, including gata1, and undergo apoptosis. Three different mon mutant alleles each encode premature stop codons, and enforced expression of wild-type tif1γ mRNA rescues embryonic hematopoiesis in homozygous mon mutants. Surprisingly, a high level of zygotic tif1γ mRNA expression delineates ventral mesoderm during hematopoietic stem cell and progenitor formation prior to gata1 expression. Transplantation studies reveal that tif1γ functions in a cell-autonomous manner during the differentiation of erythroid precursors. Studies in murine erythroid cell lines demonstrate that Tif1γ protein is localized within novel nuclear foci, and expression decreases during erythroid cell maturation. Our results establish a major role for this transcriptional intermediary factor in the differentiation of hematopoietic cells in vertebrates. A new gene acting early in red blood cell development is discovered by genetic analysis in zebrafish. The gene encodes a member of a well-known family of transcription factors Introduction Hematopoiesis involves the coordinated processes of cell proliferation and differentiation of a relatively small number of progenitor cells into billions of circulating red and white blood cells (Thisse and Zon 2002). Hematopoiesis in vertebrates, from zebrafish to humans, is an evolutionarily conserved program that produces two waves of stem or progenitor cells that differ both in their embryonic origins and in the lineages of differentiated blood cells produced (Palis and Yoder 2001; Orkin and Zon 2002; Galloway and Zon 2003). The first, or primitive, wave of hematopoiesis originates from ventral mesoderm and gives rise to progenitor cells that differentiate in embryonic blood islands. The primitive wave of hematopoiesis produces a burst of embryonic erythrocytes and macrophages. The second, or definitive, wave of hematopoiesis arises from self-renewing stem cells that develop primarily in the intraembryonic aorta–gonad–mesonephros region. These definitive hematopoietic stem cells seed the later developing marrow spaces, to produce all lineages of adult blood cells, including definitive erythrocytes, myeloid cells, and lymphocytes. We have undertaken a genetic approach to characterize genes that control hematopoiesis using the zebrafish as a model system (Thisse and Zon 2002). As part of a large-scale forward genetic screen, we previously identified bloodless zebrafish mutants that failed to express the erythroid transcription factor gata1 normally in embryonic hematopoietic precursors (Ransom et al. 1996). We named one of these zebrafish genes moonshine (mon), and another group named a noncomplementing allele vampire (Weinstein et al. 1996). Here, we have determined that mutations in the mon gene cause a disruption in both primitive embryonic and definitive adult hematopoiesis, resulting in a severe loss of erythroid cells. Erythroid progenitor cells in mon mutants are initially present, but fail to express normal levels of hematopoietic transcription factors and undergo apoptosis. Positional cloning identifies the mon gene as the zebrafish ortholog of mammalian transcriptional intermediary factor 1γ (TIF1γ), a member of the TIF1 family of transcriptional coactivators and corepressors (Le Douarin et al. 1995; Friedman et al. 1996; Kim et al. 1996; Venturini et al. 1999; Peng et al. 2002). The three members of the vertebrate TIF1 family (α, β, and γ) are large nuclear proteins that each contain an N-terminal RBCC or TRIM domain (Reymond et al. 2001) composed of a RING finger, two B-boxes, and a coiled-coil domain. TIF1 family members also contain a C-terminal plant homeodomain finger and bromodomain that are characteristic of chromatin remodeling factors. TIF1α has been shown to associate with a variety of ligand-bound nuclear hormone receptors (Le Douarin et al. 1995) and function as a coactivator for retinoic acid receptors (Zhong et al.1999). TIF1β has been shown to act as a corepressor for the large family of Krüppel-associated box (KRAB) domain zinc-finger transcription factors (Friedman et al. 1996; Abrink et al. 2001). In contrast, TIF1γ does not associate directly with either nuclear receptors or KRAB domains that bind to the other TIF1 family members (Venturini et al. 1999; Abrink et al. 2001). Biochemical studies also demonstrate that TIF1γ forms both homo-oligomers and hetero-oligomers with TIF1α but not with TIF1β (Peng et al. 2002). The murine Tif1α and Tif1γ genes have not yet been subjected to gene targeting experiments, whereas analysis of mouse mutants demonstrates that Tif1β is required for postimplantation embryogenesis and mesoderm induction in particular (Cammas et al. 2000). Taken together, these studies suggest that a major function of TIF1 family members is to link DNA-binding proteins with other coactivators or corepressors during development. Our studies establish that tif1γ functions as an essential regulator of embryonic and adult hematopoiesis in vertebrates. Cell transplantation studies demonstrate that tif1γ acts in a cell-autonomous manner during embryonic hematopoiesis. The tif1γ gene is expressed specifically in ventral mesoderm and hematopoietic progenitors, then downregulated as erythroid maturation occurs. Tif1γ protein localizes to a novel class of nuclear bodies in both primary mouse embryo fibroblasts and erythroleukemia cell lines. Taken together, our studies demonstrate that Tif1γ is required for normal erythroid cell development and survival. Results The Zebrafish mon",Gene
R1017 T4645 T4644 acomp Essential,Is
R1018 T4646 T4645 prep for,Essential
R1019 T4647 T4648 preconj Both,Primitive
R1020 T4648 T4649 amod Primitive,Erythropoiesis
R1021 T4649 T4646 pobj Erythropoiesis,for
R1022 T4650 T4648 cc and,Primitive
R1023 T4651 T4648 conj Definitive,Primitive
R1024 T4653 T4654 prep In,examined
R1025 T4655 T4653 pobj order,In
R1026 T4656 T4657 aux to,determine
R1027 T4657 T4655 acl determine,order
R1028 T4658 T4659 advmod when,required
R1029 T4659 T4657 ccomp required,determine
R1030 T4660 T4661 det the,gene
R1031 T4661 T4659 nsubjpass gene,required
R1032 T4662 T4661 compound mon,gene
R1033 T4663 T4659 auxpass is,required
R1034 T4664 T4659 prep in,required
R1035 T4665 T4664 pobj development,in
R1036 T4666 T4654 punct ", ",examined
R1037 T4667 T4654 nsubj we,examined
R1038 T4668 T4654 advmod first,examined
R1039 T4669 T4670 amod hematopoietic,expression
R1040 T4670 T4654 dobj expression,examined
R1041 T4671 T4670 compound gene,expression
R1042 T4672 T4670 cc and,expression
R1043 T4673 T4670 conj apoptosis,expression
R1044 T4674 T4654 prep in,examined
R1045 T4675 T4676 nmod zebrafish,embryos
R1046 T4676 T4674 pobj embryos,in
R1047 T4677 T4678 amod homozygous,mon
R1048 T4678 T4676 nmod mon,embryos
R1049 T4679 T4678 amod mutant,mon
R1050 T4680 T4654 punct .,examined
R1051 T4682 T4683 prep During,have
R1052 T4684 T4682 pobj embryogenesis,During
R1053 T4685 T4683 punct ", ",have
R1054 T4686 T4687 amod homozygous,mutants
R1055 T4687 T4683 nsubj mutants,have
R1056 T4688 T4687 compound zebrafish,mutants
R1057 T4689 T4687 compound mon,mutants
R1058 T4690 T4691 det no,cells
R1059 T4691 T4683 dobj cells,have
R1060 T4692 T4691 amod red,cells
R1061 T4693 T4691 compound blood,cells
R1062 T4694 T4691 punct (,cells
R1063 T4695 T4691 appos RBCs,cells
R1064 T4696 T4691 punct ),cells
R1065 T4697 T4691 amod visible,cells
R1066 T4698 T4697 prep in,visible
R1067 T4699 T4698 pobj circulation,in
R1068 T4700 T4701 punct (,Ransom
R1069 T4701 T4683 meta Ransom,have
R1070 T4702 T4701 nmod et,Ransom
R1071 T4703 T4701 nmod al.,Ransom
R1072 T4704 T4701 nummod 1996,Ransom
R1073 T4705 T4701 punct ;,Ransom
R1074 T4706 T4701 nmod Weinstein,Ransom
R1075 T4707 T4701 nmod et,Ransom
R1076 T4708 T4701 nmod al.,Ransom
R1077 T4709 T4701 nummod 1996,Ransom
R1078 T4710 T4701 punct ),Ransom
R1079 T4711 T4683 punct .,have
R1080 T4713 T4714 det The,mutants
R1081 T4714 T4716 nsubj mutants,initiate
R1082 T4715 T4714 compound mon,mutants
R1083 T4716 T4717 ccomp initiate,undergo
R1084 T4718 T4716 dobj expression,initiate
R1085 T4719 T4718 prep of,expression
R1086 T4720 T4719 pobj gata1,of
R1087 T4721 T4716 prep in,initiate
R1088 T4722 T4723 amod hematopoietic,cells
R1089 T4723 T4721 pobj cells,in
R1090 T4724 T4716 prep around,initiate
R1091 T4725 T4726 det the,stage
R1092 T4726 T4724 pobj stage,around
R1093 T4727 T4728 nummod five,somite
R1094 T4728 T4726 compound somite,stage
R1095 T4729 T4728 punct -,somite
R1096 T4730 T4716 punct ", ",initiate
R1097 T4731 T4716 advcl similar,initiate
R1098 T4732 T4731 prep to,similar
R1099 T4733 T4734 amod wild,type
R1100 T4734 T4736 compound type,embryos
R1101 T4735 T4734 punct -,type
R1102 T4736 T4732 pobj embryos,to
R1103 T4737 T4738 punct (,shown
R1104 T4738 T4716 parataxis shown,initiate
R1105 T4739 T4738 nsubj data,shown
R1106 T4740 T4738 neg not,shown
R1107 T4741 T4738 punct ),shown
R1108 T4742 T4717 punct ;,undergo
R1109 T4743 T4717 advmod however,undergo
R1110 T4744 T4717 punct ", ",undergo
R1111 T4745 T4717 prep based,undergo
R1112 T4746 T4745 prep on,based
R1113 T4747 T4748 compound TUNEL,staining
R1114 T4748 T4746 pobj staining,on
R1115 T4749 T4717 punct ", ",undergo
R1116 T4750 T4751 det the,cells
R1117 T4751 T4717 nsubj cells,undergo
R1118 T4752 T4751 amod differentiating,cells
R1119 T4753 T4751 compound erythroid,cells
R1120 T4754 T4755 amod programmed,death
R1121 T4755 T4717 dobj death,undergo
R1122 T4756 T4755 compound cell,death
R1123 T4757 T4717 prep from,undergo
R1124 T4758 T4759 det the,stage
R1125 T4759 T4757 pobj stage,from
R1126 T4760 T4761 nummod 12,somite
R1127 T4761 T4759 compound somite,stage
R1128 T4762 T4761 punct -,somite
R1129 T4763 T4757 prep to,from
R1130 T4764 T4765 nummod 22,h
R1131 T4765 T4766 compound h,postfertilization
R1132 T4766 T4763 pobj postfertilization,to
R1133 T4767 T4766 punct (,postfertilization
R1134 T4768 T4766 appos hpf,postfertilization
R1135 T4769 T4717 punct ),undergo
R1136 T4770 T4771 punct (,arrows
R1137 T4771 T4717 parataxis arrows,undergo
R1138 T4772 T4773 nmod Figure,1A
R1139 T4773 T4771 dep 1A,arrows
R1140 T4774 T4773 cc and,1A
R1141 T4775 T4773 conj 1B,1A
R1142 T4776 T4771 punct ", ",arrows
R1143 T4777 T4771 punct ),arrows
R1144 T4778 T4717 punct .,undergo
R1145 T4780 T4781 prep At,is
R1146 T4782 T4783 nummod 12,somites
R1147 T4783 T4780 pobj somites,At
R1148 T4784 T4781 punct ", ",is
R1149 T4785 T4786 compound gata1,expression
R1150 T4786 T4781 nsubj expression,is
R1151 T4787 T4788 advmod only,slightly
R1152 T4788 T4789 advmod slightly,reduced
R1153 T4789 T4781 acomp reduced,is
R1154 T4790 T4781 punct .,is
R1155 T4792 T4793 prep By,detected
R1156 T4794 T4795 quantmod 18,22
R1157 T4795 T4797 nummod 22,hpf
R1158 T4796 T4795 punct –,22
R1159 T4797 T4792 pobj hpf,By
R1160 T4798 T4793 punct ", ",detected
R1161 T4799 T4800 amod hematopoietic,specific
R1162 T4800 T4802 amod specific,markers
R1163 T4801 T4800 punct -,specific
R1164 T4802 T4793 nsubjpass markers,detected
R1165 T4803 T4804 amod such,as
R1166 T4804 T4802 prep as,markers
R1167 T4805 T4804 pobj gata1,as
R1168 T4806 T4805 punct ", ",gata1
R1169 T4807 T4805 conj scl,gata1
R1170 T4808 T4807 punct ", ",scl
R1171 T4809 T4807 conj gata2,scl
R1172 T4810 T4809 punct ", ",gata2
R1173 T4811 T4809 cc and,gata2
R1174 T4812 T4809 conj ikaros,gata2
R1175 T4813 T4793 auxpass are,detected
R1176 T4814 T4793 neg not,detected
R1177 T4815 T4793 prep in,detected
R1178 T4816 T4817 det the,island
R1179 T4817 T4815 pobj island,in
R1180 T4818 T4817 amod embryonic,island
R1181 T4819 T4817 compound blood,island
R1182 T4820 T4821 punct (,data
R1183 T4821 T4793 parataxis data,detected
R1184 T4822 T4823 nmod Figure,1A
R1185 T4823 T4821 dep 1A,data
R1186 T4824 T4823 cc and,1A
R1187 T4825 T4823 conj 1B,1A
R1188 T4826 T4821 punct ;,data
R1189 T4827 T4821 amod unpublished,data
R1190 T4828 T4821 punct ),data
R1191 T4829 T4793 punct .,detected
R1192 T4831 T4832 det The,cells
R1193 T4832 T4834 nsubjpass cells,specified
R1194 T4833 T4832 amod hematopoietic,cells
R1195 T4835 T4834 auxpass are,specified
R1196 T4836 T4834 advmod thus,specified
R1197 T4837 T4834 advmod correctly,specified
R1198 T4838 T4834 advmod early,specified
R1199 T4839 T4838 prep during,early
R1200 T4840 T4841 det the,development
R1201 T4841 T4839 pobj development,during
R1202 T4842 T4841 prep of,development
R1203 T4843 T4844 nmod mon,embryos
R1204 T4844 T4842 pobj embryos,of
R1205 T4845 T4844 amod mutant,embryos
R1206 T4846 T4834 punct ", ",specified
R1207 T4847 T4834 cc but,specified
R1208 T4848 T4849 det these,precursors
R1209 T4849 T4850 nsubj precursors,undergo
R1210 T4850 T4834 conj undergo,specified
R1211 T4851 T4852 compound cell,death
R1212 T4852 T4850 dobj death,undergo
R1213 T4853 T4850 punct .,undergo
R1214 T4855 T4856 prep Based,have
R1215 T4857 T4855 prep on,Based
R1216 T4858 T4857 pobj expression,on
R1217 T4859 T4858 prep of,expression
R1218 T4860 T4861 compound c,myb
R1219 T4861 T4859 pobj myb,of
R1220 T4862 T4861 punct -,myb
R1221 T4863 T4861 cc and,myb
R1222 T4864 T4861 conj rag1,myb
R1223 T4865 T4866 punct (,arrows
R1224 T4866 T4856 parataxis arrows,have
R1225 T4867 T4866 dep Figure,arrows
R1226 T4868 T4867 nummod 1B,Figure
R1227 T4869 T4866 punct ", ",arrows
R1228 T4870 T4866 punct ),arrows
R1229 T4871 T4856 punct ", ",have
R1230 T4872 T4873 compound mon,mutants
R1231 T4873 T4856 nsubj mutants,have
R1232 T4874 T4875 amod normal,development
R1233 T4875 T4856 dobj development,have
R1234 T4876 T4875 amod myeloid,development
R1235 T4877 T4876 cc and,myeloid
R1236 T4878 T4876 conj lymphoid,myeloid
R1237 T4879 T4856 punct ", ",have
R1238 T4880 T4856 advmod respectively,have
R1239 T4881 T4856 punct .,have
R1240 T4883 T4884 prep In,is
R1241 T4885 T4883 pobj addition,In
R1242 T4886 T4885 prep to,addition
R1243 T4887 T4888 det the,deficit
R1244 T4888 T4886 pobj deficit,to
R1245 T4889 T4888 prep of,deficit
R1246 T4890 T4889 pobj RBCs,of
R1247 T4891 T4888 prep in,deficit
R1248 T4892 T4893 compound mon,mutants
R1249 T4893 T4891 pobj mutants,in
R1250 T4894 T4884 punct ", ",is
R1251 T4895 T4884 expl there,is
R1252 T4896 T4897 det a,loss
R1253 T4897 T4884 attr loss,is
R1254 T4898 T4897 amod prominent,loss
R1255 T4899 T4897 prep of,loss
R1256 T4900 T4901 nmod fin,fold
R1257 T4901 T4903 nmod fold,mesenchyme
R1258 T4902 T4901 punct -,fold
R1259 T4903 T4899 pobj mesenchyme,of
R1260 T4904 T4901 cc and,fold
R1261 T4905 T4901 conj tail,fold
R1262 T4906 T4907 punct (,Ransom
R1263 T4907 T4884 meta Ransom,is
R1264 T4908 T4907 nmod et,Ransom
R1265 T4909 T4907 nmod al.,Ransom
R1266 T4910 T4907 nummod 1996,Ransom
R1267 T4911 T4907 punct ),Ransom
R1268 T4912 T4884 punct .,is
R1269 T4914 T4915 compound TUNEL,staining
R1270 T4915 T4916 nsubj staining,demonstrates
R1271 T4917 T4915 prep of,staining
R1272 T4918 T4919 compound mon,mutants
R1273 T4919 T4917 pobj mutants,of
R1274 T4920 T4921 amod extensive,apoptosis
R1275 T4921 T4916 dobj apoptosis,demonstrates
R1276 T4922 T4921 prep of,apoptosis
R1277 T4923 T4924 amod mesenchymal,cells
R1278 T4924 T4922 pobj cells,of
R1279 T4925 T4916 prep in,demonstrates
R1280 T4926 T4927 det the,regions
R1281 T4927 T4925 pobj regions,in
R1282 T4928 T4927 nmod trunk,regions
R1283 T4929 T4928 cc and,trunk
R1284 T4930 T4931 compound tail,bud
R1285 T4931 T4928 conj bud,trunk
R1286 T4932 T4933 punct (,arrows
R1287 T4933 T4916 parataxis arrows,demonstrates
R1288 T4934 T4935 nmod Figure,1A
R1289 T4935 T4933 dep 1A,arrows
R1290 T4936 T4935 cc and,1A
R1291 T4937 T4935 conj 1B,1A
R1292 T4938 T4933 punct ", ",arrows
R1293 T4939 T4933 punct ),arrows
R1294 T4940 T4916 punct .,demonstrates
R1295 T4942 T4943 det The,gene
R1296 T4943 T4945 nsubjpass gene,required
R1297 T4944 T4943 compound mon,gene
R1298 T4946 T4945 auxpass is,required
R1299 T4947 T4945 advmod thus,required
R1300 T4948 T4945 prep for,required
R1301 T4949 T4950 amod normal,development
R1302 T4950 T4948 pobj development,for
R1303 T4951 T4950 cc and,development
R1304 T4952 T4950 conj survival,development
R1305 T4953 T4950 prep of,development
R1306 T4954 T4955 preconj both,cells
R1307 T4955 T4953 pobj cells,of
R1308 T4956 T4955 amod committed,cells
R1309 T4957 T4955 amod erythroid,cells
R1310 T4958 T4955 compound progenitor,cells
R1311 T4959 T4955 cc and,cells
R1312 T4960 T4961 amod posterior,cells
R1313 T4961 T4955 conj cells,cells
R1314 T4962 T4961 amod mesenchymal,cells
R1315 T4963 T4945 punct .,required
R1316 T4965 T4966 nsubj We,examined
R1317 T4967 T4966 advmod next,examined
R1318 T4968 T4969 amod definitive,hematopoiesis
R1319 T4969 T4966 dobj hematopoiesis,examined
R1320 T4970 T4966 prep in,examined
R1321 T4971 T4972 amod rare,mutants
R1322 T4972 T4970 pobj mutants,in
R1323 T4973 T4972 amod surviving,mutants
R1324 T4974 T4975 amod homozygous,mon
R1325 T4975 T4972 compound mon,mutants
R1326 T4976 T4975 amod adult,mon
R1327 T4977 T4975 compound zebrafish,mon
R1328 T4978 T4966 punct .,examined
R1329 T4980 T4981 nsubj Mutations,are
R1330 T4982 T4980 prep in,Mutations
R1331 T4983 T4982 pobj mon,in
R1332 T4984 T4981 advmod generally,are
R1333 T4985 T4981 acomp lethal,are
R1334 T4986 T4981 prep by,are
R1335 T4987 T4988 quantmod 10,14
R1336 T4988 T4990 nummod 14,d
R1337 T4989 T4988 quantmod to,14
R1338 T4990 T4986 pobj d,by
R1339 T4991 T4990 prep of,d
R1340 T4992 T4991 pobj development,of
R1341 T4993 T4994 punct (,Ransom
R1342 T4994 T4981 meta Ransom,are
R1343 T4995 T4994 nmod et,Ransom
R1344 T4996 T4994 nmod al.,Ransom
R1345 T4997 T4994 nummod 1996,Ransom
R1346 T4998 T4994 punct ),Ransom
R1347 T4999 T4981 punct ", ",are
R1348 T5000 T5001 mark although,survive
R1349 T5001 T4981 advcl survive,are
R1350 T5002 T5003 amod rare,mutants
R1351 T5003 T5001 nsubj mutants,survive
R1352 T5004 T5003 nmod mon,mutants
R1353 T5005 T5003 amod homozygous,mutants
R1354 T5006 T5007 punct (,embryos
R1355 T5007 T5003 parataxis embryos,mutants
R1356 T5008 T5009 advmod approximately,500
R1357 T5009 T5007 nummod 500,embryos
R1358 T5010 T5009 quantmod 1,500
R1359 T5011 T5009 quantmod in,500
R1360 T5012 T5007 amod bloodless,embryos
R1361 T5013 T5007 punct ),embryos
R1362 T5014 T5003 prep of,mutants
R1363 T5015 T5016 det all,alleles
R1364 T5016 T5014 pobj alleles,of
R1365 T5017 T5016 amod tested,alleles
R1366 T5018 T5001 prep to,survive
R1367 T5019 T5018 pobj adulthood,to
R1368 T5020 T4981 punct .,are
R1369 T5022 T5023 amod Adult,mutants
R1370 T5023 T5025 nsubj mutants,show
R1371 T5024 T5023 compound mon,mutants
R1372 T5026 T5027 amod cardiac,hypertrophy
R1373 T5027 T5025 dobj hypertrophy,show
R1374 T5028 T5025 punct ", ",show
R1375 T5029 T5030 advmod presumably,due
R1376 T5030 T5025 prep due,show
R1377 T5031 T5030 pcomp to,due
R1378 T5032 T5033 det the,anemia
R1379 T5033 T5030 pobj anemia,due
R1380 T5034 T5033 amod severe,anemia
R1381 T5035 T5025 advcl leading,show
R1382 T5036 T5035 prep to,leading
R1383 T5037 T5038 det a,state
R1384 T5038 T5036 pobj state,to
R1385 T5039 T5040 amod high,output
R1386 T5040 T5038 compound output,state
R1387 T5041 T5042 punct (,Figure
R1388 T5042 T5025 parataxis Figure,show
R1389 T5043 T5042 nummod 2,Figure
R1390 T5044 T5042 punct ),Figure
R1391 T5045 T5025 punct .,show
R1392 T5047 T5048 prep In,is
R1393 T5049 T5050 amod wild,type
R1394 T5050 T5052 compound type,zebrafish
R1395 T5051 T5050 punct -,type
R1396 T5052 T5047 pobj zebrafish,In
R1397 T5053 T5048 punct ", ",is
R1398 T5054 T5055 det the,site
R1399 T5055 T5048 nsubj site,is
R1400 T5056 T5055 amod adult,site
R1401 T5057 T5055 prep of,site
R1402 T5058 T5057 pobj hematopoiesis,of
R1403 T5059 T5060 det the,kidney
R1404 T5060 T5048 attr kidney,is
R1405 T5061 T5062 punct (,Al
R1406 T5062 T5060 meta Al,kidney
R1407 T5063 T5062 nmod Adhami,Al
R1408 T5064 T5062 cc and,Al
R1409 T5065 T5062 conj Kunz,Al
R1410 T5066 T5065 nummod 1977,Kunz
R1411 T5067 T5065 punct ),Kunz
R1412 T5068 T5060 punct ", ",kidney
R1413 T5069 T5070 dep which,contains
R1414 T5070 T5060 relcl contains,kidney
R1415 T5071 T5072 amod erythroid,populations
R1416 T5072 T5070 dobj populations,contains
R1417 T5073 T5071 punct ", ",erythroid
R1418 T5074 T5071 conj lymphoid,erythroid
R1419 T5075 T5074 punct ", ",lymphoid
R1420 T5076 T5074 cc and,lymphoid
R1421 T5077 T5074 conj myeloid,lymphoid
R1422 T5078 T5070 prep at,contains
R1423 T5079 T5080 amod various,stages
R1424 T5080 T5078 pobj stages,at
R1425 T5081 T5080 prep of,stages
R1426 T5082 T5081 pobj differentiation,of
R1427 T5083 T5084 punct (,Bennett
R1428 T5084 T5070 meta Bennett,contains
R1429 T5085 T5084 nmod et,Bennett
R1430 T5086 T5084 nmod al.,Bennett
R1431 T5087 T5084 nummod 2001,Bennett
R1432 T5088 T5084 punct ),Bennett
R1433 T5089 T5048 punct .,is
R1434 T5091 T5092 prep In,is
R1435 T5093 T5094 npadvmod mon,homozygous
R1436 T5094 T5095 amod homozygous,mutants
R1437 T5095 T5091 pobj mutants,In
R1438 T5096 T5092 punct ", ",is
R1439 T5097 T5092 expl there,is
R1440 T5098 T5099 det a,block
R1441 T5099 T5092 attr block,is
R1442 T5100 T5099 amod severe,block
R1443 T5101 T5099 prep in,block
R1444 T5102 T5101 pobj maturation,in
R1445 T5103 T5092 prep at,is
R1446 T5104 T5105 det the,stage
R1447 T5105 T5103 pobj stage,at
R1448 T5106 T5105 compound proerythroblast,stage
R1449 T5107 T5108 punct (,Figure
R1450 T5108 T5092 parataxis Figure,is
R1451 T5109 T5108 nummod 2,Figure
R1452 T5110 T5108 punct ),Figure
R1453 T5111 T5092 punct ", ",is
R1454 T5112 T5113 mark whereas,is
R1455 T5113 T5092 advcl is,is
R1456 T5114 T5115 det the,differentiation
R1457 T5115 T5113 nsubj differentiation,is
R1458 T5116 T5115 prep of,differentiation
R1459 T5117 T5118 amod myeloid,cells
R1460 T5118 T5116 pobj cells,of
R1461 T5119 T5113 acomp normal,is
R1462 T5120 T5121 punct (,data
R1463 T5121 T5113 parataxis data,is
R1464 T5122 T5121 amod unpublished,data
R1465 T5123 T5121 punct ),data
R1466 T5124 T5092 punct .,is
R1467 T5126 T5127 nsubj This,demonstrates
R1468 T5128 T5129 mark that,acts
R1469 T5129 T5127 ccomp acts,demonstrates
R1470 T5130 T5131 det the,product
R1471 T5131 T5129 nsubj product,acts
R1472 T5132 T5133 compound mon,gene
R1473 T5133 T5131 compound gene,product
R1474 T5134 T5129 prep during,acts
R1475 T5135 T5136 preconj both,primitive
R1476 T5136 T5137 amod primitive,erythropoiesis
R1477 T5137 T5134 pobj erythropoiesis,during
R1478 T5138 T5136 cc and,primitive
R1479 T5139 T5136 conj definitive,primitive
R1480 T5140 T5127 punct .,demonstrates
R1498 T7933 T7931 dobj allele,carrying
R1499 T7892 T7893 amod Positional,cloning
R1500 T7893 T7894 nsubj cloning,identifies
R1501 T7895 T7894 dobj mon,identifies
R1502 T7896 T7894 prep as,identifies
R1503 T7897 T7898 det the,Ortholog
R1504 T7898 T7896 pobj Ortholog,as
R1505 T7899 T7898 compound Zebrafish,Ortholog
R1506 T7900 T7898 prep of,Ortholog
R1507 T7901 T7902 amod Mammalian,TIF1γ
R1508 T7902 T7900 pobj TIF1γ,of
R1509 T7904 T7905 nsubj We,identified
R1510 T7906 T7907 det the,gene
R1511 T7907 T7905 dobj gene,identified
R1512 T7908 T7907 compound mon,gene
R1513 T7909 T7905 prep by,identified
R1514 T7910 T7911 amod positional,cloning
R1515 T7911 T7909 pobj cloning,by
R1516 T7934 T7933 compound montg234,allele
R1517 T7912 T7905 advcl using,identified
R1518 T7913 T7914 det a,panel
R1519 T7914 T7912 dobj panel,using
R1520 T7915 T7914 prep of,panel
R1521 T7935 T7905 punct .,identified
R1522 T7916 T7917 nummod "2,200",mutants
R1523 T7917 T7915 pobj mutants,of
R1524 T7918 T7917 amod diploid,mutants
R1525 T7937 T7938 det The,gene
R1526 T7919 T7917 acl collected,mutants
R1527 T7920 T7919 prep from,collected
R1528 T7921 T7922 nmod Tübingen,background
R1529 T7922 T7923 nmod background,strain
R1530 T7923 T7929 compound strain,hybrid
R1531 T7924 T7922 punct (,background
R1532 T7925 T7922 appos TU,background
R1533 T7926 T7922 punct ),background
R1534 T7927 T7922 punct /,background
R1535 T7928 T7922 appos WIK,background
R1536 T7929 T7930 compound hybrid,parents
R1537 T7930 T7920 pobj parents,from
R1538 T7938 T7941 nsubjpass gene,positioned
R1539 T7931 T7930 acl carrying,parents
R1540 T7932 T7933 det the,allele
R1541 T7939 T7938 nmod mon,gene
R1542 T7940 T7938 amod mutant,gene
R1543 T7942 T7941 auxpass was,positioned
R1544 T7943 T7941 prep on,positioned
R1545 T8040 T8033 punct (,clone
R1546 T7944 T7943 pobj Chromosome,on
R1547 T8041 T8033 appos PAC,clone
R1548 T8042 T8033 punct ),clone
R1549 T7945 T7944 nummod 8,Chromosome
R1550 T8043 T8033 punct ", ",clone
R1551 T8044 T8033 appos 107N19,clone
R1552 T7946 T7941 prep between,positioned
R1553 T8045 T8039 punct ", ",obtained
R1554 T8046 T8039 auxpass was,obtained
R1555 T8047 T8048 dep that,spanned
R1556 T7947 T7948 compound microsatellite,markers
R1557 T8048 T8039 ccomp spanned,obtained
R1558 T8049 T8050 det the,interval
R1559 T8050 T8048 dobj interval,spanned
R1560 T7948 T7946 pobj markers,between
R1561 T8051 T8050 amod genetic,interval
R1562 T8052 T8039 punct .,obtained
R1563 T7949 T7948 appos z987,markers
R1564 T8054 T8055 nummod Two,markers
R1565 T8055 T8063 nsubj markers,flank
R1566 T8056 T8057 amod simple,polymorphism
R1567 T7950 T7949 cc and,z987
R1568 T8057 T8055 nmod polymorphism,markers
R1569 T8058 T8057 nmod sequence,polymorphism
R1570 T8059 T8057 nmod conformation,polymorphism
R1571 T7951 T7949 conj z11001,z987
R1572 T8060 T8057 punct (,polymorphism
R1573 T8061 T8057 appos SSCP,polymorphism
R1574 T8062 T8055 punct ),markers
R1575 T7952 T7953 punct (,Figure
R1576 T8064 T8055 acl found,markers
R1577 T8065 T8064 prep on,found
R1578 T7953 T7941 parataxis Figure,positioned
R1579 T8066 T8067 det this,clone
R1580 T8067 T8065 pobj clone,on
R1581 T8068 T8067 compound PAC,clone
R1582 T8069 T8070 det the,interval
R1583 T8070 T8063 dobj interval,flank
R1584 T8071 T8070 amod critical,interval
R1585 T7954 T7953 nummod 3A,Figure
R1586 T8072 T8070 amod genetic,interval
R1587 T8073 T8063 punct .,flank
R1588 T7955 T7953 punct ),Figure
R1589 T8075 T8076 det The,marker
R1590 T8076 T8077 nsubj marker,maps
R1591 T7956 T7957 punct (,Knapik
R1592 T8078 T8079 compound 80M12,T7
R1593 T8079 T8076 appos T7,marker
R1594 T7957 T7941 meta Knapik,positioned
R1595 T8080 T8079 punct -,T7
R1596 T8081 T8082 nummod two,recombinants
R1597 T8082 T8077 dobj recombinants,maps
R1598 T7958 T7957 nmod et,Knapik
R1599 T8083 T8077 prep out,maps
R1600 T8084 T8083 prep of,out
R1601 T8085 T8086 nummod "4,400",meioses
R1602 T7959 T7957 nmod al.,Knapik
R1603 T8086 T8084 pobj meioses,of
R1604 T8087 T8086 amod telomeric,meioses
R1605 T8088 T8086 prep of,meioses
R1606 T7960 T7957 nummod 1998,Knapik
R1607 T8089 T8090 det the,mutation
R1608 T8090 T8088 pobj mutation,of
R1609 T8091 T8077 punct ", ",maps
R1610 T7961 T7957 punct ),Knapik
R1611 T8092 T8077 cc and,maps
R1612 T8093 T8094 det the,marker
R1613 T8094 T8095 nsubj marker,maps
R1614 T7962 T7941 punct .,positioned
R1615 T8095 T8077 conj maps,maps
R1616 T8096 T8097 compound 157J23,T7
R1617 T7964 T7965 prep For,screened
R1618 T8097 T8094 appos T7,marker
R1619 T8098 T8097 punct -,T7
R1620 T8099 T8100 nummod one,centromic
R1621 T8100 T8095 dobj centromic,maps
R1622 T8101 T8100 amod recombinant,centromic
R1623 T7966 T7967 amod positional,purposes
R1624 T7967 T7964 pobj purposes,For
R1625 T8102 T8100 prep of,centromic
R1626 T7968 T7967 compound cloning,purposes
R1627 T8103 T8104 det the,mutation
R1628 T8104 T8102 pobj mutation,of
R1629 T8105 T8106 punct (,Figure
R1630 T7969 T7965 punct ", ",screened
R1631 T8106 T8095 parataxis Figure,maps
R1632 T8107 T8106 nummod 3A,Figure
R1633 T8108 T8106 punct ),Figure
R1634 T8109 T8095 punct .,maps
R1635 T8111 T8112 det The,sequences
R1636 T7970 T7971 quantmod over,"12,000"
R1637 T8112 T8114 nsubjpass sequences,found
R1638 T8113 T8112 compound end,sequences
R1639 T7971 T7972 nummod "12,000",markers
R1640 T8115 T8112 cc and,sequences
R1641 T8116 T8117 compound SSCP,markers
R1642 T7972 T7965 nsubjpass markers,screened
R1643 T8117 T8112 conj markers,sequences
R1644 T8118 T8112 prep of,sequences
R1645 T8119 T8120 compound PAC,107N19
R1646 T8120 T8118 pobj 107N19,of
R1647 T7973 T7972 amod polymorphic,markers
R1648 T8121 T8114 auxpass are,found
R1649 T8122 T8114 prep in,found
R1650 T7974 T7965 auxpass were,screened
R1651 T7975 T7965 advcl using,screened
R1652 T8123 T8124 det the,contig
R1653 T7976 T7977 amod amplified,polymorphism
R1654 T8124 T8122 pobj contig,in
R1655 T8125 T8124 nmod zebrafish,contig
R1656 T8126 T8124 amod genomic,contig
R1657 T7977 T7975 dobj polymorphism,using
R1658 T8127 T8124 compound sequence,contig
R1659 T8128 T8124 appos ctg23107,contig
R1660 T8129 T8130 punct (,http://www.ensembl.org/Danio_rerio/
R1661 T7978 T7977 compound fragment,polymorphism
R1662 T8130 T8124 parataxis http://www.ensembl.org/Danio_rerio/,contig
R1663 T8131 T8130 punct ),http://www.ensembl.org/Danio_rerio/
R1664 T7979 T7977 compound length,polymorphism
R1665 T8132 T8124 acl containing,contig
R1666 T8133 T8134 det a,gene
R1667 T8134 T8132 dobj gene,containing
R1668 T7980 T7977 punct (,polymorphism
R1669 T8135 T8134 amod predicted,gene
R1670 T8136 T8134 compound zebrafish,gene
R1671 T8137 T8138 compound TIF1,family
R1672 T7981 T7977 appos AFLP,polymorphism
R1673 T8138 T8134 compound family,gene
R1674 T8139 T8114 punct .,found
R1675 T7982 T7975 punct ),using
R1676 T8141 T8142 det This,PAC
R1677 T8142 T8143 nsubjpass PAC,hybridized
R1678 T7983 T7984 punct (,Ransom
R1680 T7984 T7965 meta Ransom,screened
R1681 T7985 T7984 cc and,Ransom
R1682 T7986 T7984 conj Zon,Ransom
R1683 T8145 T8143 prep to,hybridized
R1684 T7987 T7986 nummod 1999,Zon
R1685 T8146 T8147 det a,library
R1686 T8147 T8145 pobj library,to
R1687 T8148 T8147 compound kidney,library
R1688 T8149 T8147 compound cDNA,library
R1689 T8150 T8143 punct ", ",hybridized
R1690 T8151 T8143 advcl resulting,hybridized
R1691 T7988 T7986 punct ),Zon
R1692 T8152 T8151 prep in,resulting
R1693 T8153 T8154 det the,isolation
R1694 T8154 T8152 pobj isolation,in
R1695 T7989 T7965 punct ", ",screened
R1696 T8155 T8154 prep of,isolation
R1697 T8156 T8157 nummod four,clones
R1698 T7990 T7965 cc and,screened
R1699 T8157 T8155 pobj clones,of
R1700 T8158 T8159 dep that,represented
R1701 T8159 T8157 relcl represented,clones
R1702 T7991 T7992 nummod 36,markers
R1703 T8160 T8161 det the,gene
R1704 T8161 T8159 dobj gene,represented
R1705 T7992 T7993 nsubjpass markers,isolated
R1706 T7993 T7965 conj isolated,screened
R1707 T8162 T8161 amod same,gene
R1708 T8163 T8143 punct .,hybridized
R1709 T7994 T7992 prep within,markers
R1710 T8165 T8166 det The,gene
R1711 T7995 T7996 det the,interval
R1712 T8166 T8168 nsubj gene,encodes
R1713 T8167 T8166 compound mon,gene
R1714 T7996 T7994 pobj interval,within
R1715 T8169 T8170 det a,member
R1716 T8170 T8168 dobj member,encodes
R1717 T8171 T8170 prep of,member
R1718 T7997 T7993 auxpass were,isolated
R1719 T8172 T8173 det the,family
R1720 T8173 T8171 pobj family,of
R1721 T8174 T8173 compound TIF1,family
R1722 T7998 T7965 punct .,screened
R1723 T8175 T8173 prep of,family
R1724 T8176 T8177 amod transcriptional,cofactors
R1725 T8000 T8001 nsubjpass One,found
R1726 T8177 T8175 pobj cofactors,of
R1727 T8178 T8179 punct (,Figure
R1728 T8179 T8168 parataxis Figure,encodes
R1729 T8180 T8179 nummod 3B,Figure
R1730 T8002 T8000 prep of,One
R1731 T8181 T8179 cc and,Figure
R1732 T8182 T8179 conj 3C,Figure
R1733 T8183 T8179 punct ),Figure
R1734 T8184 T8168 punct .,encodes
R1735 T8003 T8002 pobj these,of
R1736 T8186 T8187 det The,sequence
R1737 T8187 T8189 nsubj sequence,is
R1738 T8188 T8187 amod coding,sequence
R1739 T8190 T8187 prep of,sequence
R1740 T8004 T8000 punct ", ",One
R1741 T8191 T8190 pobj mon,of
R1742 T8192 T8193 advmod most,similar
R1743 T8193 T8189 acomp similar,is
R1744 T8194 T8193 prep to,similar
R1745 T8005 T8000 appos MA3,One
R1746 T8195 T8196 amod human,TIFγ
R1747 T8196 T8194 pobj TIFγ,to
R1748 T8197 T8198 punct (,Le
R1749 T8006 T8001 punct ", ",found
R1750 T8198 T8189 meta Le,is
R1751 T8199 T8198 nmod Douarin,Le
R1752 T8200 T8198 nmod et,Le
R1753 T8007 T8001 auxpass was,found
R1754 T8201 T8198 nmod al.,Le
R1755 T8202 T8198 nummod 1995,Le
R1756 T8008 T8009 aux to,be
R1757 T8203 T8198 punct ;,Le
R1758 T8204 T8198 nmod Friedman,Le
R1759 T8205 T8198 nmod et,Le
R1760 T8009 T8001 xcomp be,found
R1761 T8206 T8198 nmod al.,Le
R1762 T8207 T8198 nummod 1996,Le
R1763 T8208 T8198 punct ;,Le
R1764 T8010 T8011 nummod 0.3,cM
R1765 T8209 T8198 nmod Venturini,Le
R1766 T8011 T8012 npadvmod cM,from
R1767 T8012 T8009 prep from,be
R1768 T8210 T8198 nmod et,Le
R1769 T8211 T8198 nmod al.,Le
R1770 T8013 T8014 det the,gene
R1771 T8212 T8198 nummod 1999,Le
R1772 T8213 T8198 punct ),Le
R1773 T8214 T8189 punct ", ",is
R1774 T8014 T8012 pobj gene,from
R1775 T8215 T8189 cc and,is
R1776 T8216 T8217 det the,locations
R1777 T8015 T8016 punct (,Figure
R1778 T8217 T8218 nsubjpass locations,conserved
R1779 T8218 T8189 conj conserved,is
R1780 T8016 T8001 parataxis Figure,found
R1781 T8219 T8217 prep of,locations
R1782 T8220 T8221 compound exon,boundaries
R1783 T8221 T8219 pobj boundaries,of
R1784 T8017 T8016 nummod 3A,Figure
R1785 T8222 T8218 auxpass are,conserved
R1786 T8223 T8218 prep between,conserved
R1787 T8224 T8225 det the,genes
R1788 T8018 T8016 punct ),Figure
R1789 T8225 T8223 pobj genes,between
R1790 T8226 T8225 nmod zebrafish,genes
R1791 T8227 T8226 cc and,zebrafish
R1792 T8228 T8226 conj human,zebrafish
R1793 T8229 T8230 punct (,data
R1794 T8230 T8218 parataxis data,conserved
R1795 T8019 T8001 cc and,found
R1796 T8231 T8230 amod unpublished,data
R1797 T8232 T8230 punct ),data
R1798 T8233 T8218 punct .,conserved
R1799 T8020 T8021 auxpass was,utilized
R1800 T8235 T8236 det The,locus
R1801 T8236 T8238 nsubjpass locus,predicted
R1802 T8021 T8001 conj utilized,found
R1803 T8237 T8236 compound mon,locus
R1804 T8239 T8236 prep on,locus
R1805 T8022 T8021 prep as,utilized
R1806 T8240 T8241 compound zebrafish,Chromosome
R1807 T8241 T8239 pobj Chromosome,on
R1808 T8023 T8024 det the,point
R1809 T8242 T8241 nummod 8,Chromosome
R1810 T8243 T8238 auxpass is,predicted
R1811 T8244 T8238 advmod also,predicted
R1812 T8245 T8246 aux to,be
R1813 T8024 T8022 pobj point,as
R1814 T8246 T8238 xcomp be,predicted
R1815 T8247 T8246 acomp syntenic,be
R1816 T8248 T8247 prep to,syntenic
R1817 T8025 T8024 amod starting,point
R1818 T8249 T8250 det the,region
R1819 T8250 T8248 pobj region,to
R1820 T8026 T8024 prep of,point
R1821 T8027 T8028 det a,walk
R1822 T8028 T8026 pobj walk,of
R1823 T8251 T8250 prep of,region
R1824 T8252 T8253 amod human,Chromosome
R1825 T8029 T8028 amod chromosomal,walk
R1826 T8253 T8251 pobj Chromosome,of
R1827 T8254 T8253 nummod 1p,Chromosome
R1828 T8255 T8256 dep that,contains
R1829 T8030 T8001 punct .,found
R1830 T8256 T8253 relcl contains,Chromosome
R1831 T8257 T8258 det the,gene
R1832 T8258 T8256 dobj gene,contains
R1833 T8032 T8033 det A,clone
R1834 T8259 T8258 compound TIF1γ,gene
R1835 T8260 T8238 prep based,predicted
R1836 T8261 T8260 prep on,based
R1837 T8262 T8263 det the,locations
R1838 T8033 T8039 nsubjpass clone,obtained
R1839 T8263 T8261 pobj locations,on
R1840 T8264 T8263 amod conserved,locations
R1841 T8265 T8263 prep of,locations
R1842 T8266 T8267 nummod 12,pairs
R1843 T8267 T8265 pobj pairs,of
R1844 T8268 T8267 amod other,pairs
R1845 T8034 T8033 amod critical,clone
R1846 T8269 T8267 amod orthologous,pairs
R1847 T8270 T8267 compound gene,pairs
R1848 T8271 T8267 punct ", ",pairs
R1849 T8272 T8267 prep including,pairs
R1850 T8035 T8033 nmod P1,clone
R1851 T8273 T8272 pobj NRAS,including
R1852 T8274 T8267 punct ", ",pairs
R1853 T8275 T8267 acl mapped,pairs
R1854 T8036 T8033 amod bacterial,clone
R1855 T8276 T8275 prep to,mapped
R1856 T8277 T8278 det these,regions
R1857 T8278 T8276 pobj regions,to
R1858 T8037 T8033 amod artificial,clone
R1859 T8279 T8278 prep in,regions
R1860 T8280 T8281 amod human,zebrafish
R1861 T8281 T8279 pobj zebrafish,in
R1862 T8038 T8033 compound chromosome,clone
R1863 T8282 T8281 cc and,zebrafish
R1864 T8283 T8284 punct (,Barbazuk
R1865 T8284 T8238 meta Barbazuk,predicted
R1866 T8357 T8358 det a,phenotype
R1867 T8285 T8284 nmod et,Barbazuk
R1868 T8286 T8284 nmod al.,Barbazuk
R1869 T8287 T8284 nummod 2000,Barbazuk
R1870 T8288 T8284 punct ),Barbazuk
R1871 T8358 T8356 dobj phenotype,have
R1872 T8289 T8238 punct .,predicted
R1873 T8291 T8292 advmod Therefore,is
R1874 T8359 T8358 amod severe,phenotype
R1875 T8293 T8292 punct ", ",is
R1876 T8294 T8292 prep based,is
R1877 T8360 T8358 prep with,phenotype
R1878 T8361 T8362 det no,cells
R1879 T8295 T8294 prep on,based
R1880 T8362 T8360 pobj cells,with
R1881 T8296 T8297 compound sequence,similarity
R1882 T8297 T8295 pobj similarity,on
R1883 T8298 T8297 cc and,similarity
R1884 T8363 T8362 amod circulating,cells
R1885 T8299 T8300 amod chromosomal,location
R1886 T8300 T8297 conj location,similarity
R1887 T8301 T8292 punct ", ",is
R1888 T8364 T8362 compound blood,cells
R1889 T8302 T8303 det the,gene
R1890 T8303 T8292 nsubj gene,is
R1891 T8365 T8356 punct .,have
R1892 T8304 T8303 compound zebrafish,gene
R1893 T8305 T8303 compound mon,gene
R1894 T8306 T8307 det the,ortholog
R1895 T8307 T8292 attr ortholog,is
R1896 T8308 T8307 amod likely,ortholog
R1897 T8309 T8307 prep of,ortholog
R1898 T8367 T8368 prep In,has
R1899 T8310 T8311 det the,gene
R1900 T8311 T8309 pobj gene,of
R1901 T8312 T8311 amod human,gene
R1902 T8313 T8311 compound TIF1γ,gene
R1903 T8314 T8292 punct .,is
R1904 T8369 T8367 pobj contrast,In
R1905 T8316 T8317 nsubj We,identified
R1906 T8318 T8317 aux have,identified
R1907 T8370 T8368 punct ", ",has
R1908 T8319 T8320 compound ethyl,nitrosourea
R1909 T8320 T8322 npadvmod nitrosourea,induced
R1910 T8321 T8320 punct -,nitrosourea
R1911 T8371 T8372 det the,allele
R1912 T8322 T8327 amod induced,mutations
R1913 T8323 T8320 punct (,nitrosourea
R1914 T8324 T8320 appos ENU,nitrosourea
R1915 T8372 T8368 nsubj allele,has
R1916 T8325 T8322 punct ),induced
R1917 T8326 T8322 punct -,induced
R1918 T8373 T8372 compound monm262,allele
R1919 T8327 T8317 dobj mutations,identified
R1920 T8328 T8327 compound point,mutations
R1921 T8329 T8317 prep in,identified
R1922 T8374 T8375 quantmod 10,100
R1923 T8330 T8331 nummod three,alleles
R1924 T8331 T8329 pobj alleles,in
R1925 T8332 T8331 prep of,alleles
R1926 T8375 T8377 nummod 100,cells
R1927 T8333 T8332 pobj mon,of
R1928 T8334 T8335 punct (,3C
R1929 T8335 T8317 parataxis 3C,identified
R1930 T8376 T8375 punct –,100
R1931 T8336 T8335 dep Figure,3C
R1932 T8337 T8335 cc and,3C
R1933 T8338 T8335 conj 3D,3C
R1934 T8377 T8368 dobj cells,has
R1935 T8339 T8335 punct ),3C
R1936 T8340 T8317 punct ", ",identified
R1937 T8341 T8342 dep each,generates
R1938 T8378 T8377 amod circulating,cells
R1939 T8342 T8317 ccomp generates,identified
R1940 T8343 T8341 prep of,each
R1941 T8344 T8343 pobj which,of
R1942 T8379 T8377 compound blood,cells
R1943 T8345 T8346 det a,codon
R1944 T8346 T8342 dobj codon,generates
R1945 T8347 T8346 amod premature,codon
R1946 T8348 T8346 compound stop,codon
R1947 T8349 T8317 punct .,identified
R1948 T8380 T8368 prep by,has
R1949 T8351 T8352 det The,alleles
R1950 T8352 T8356 nsubj alleles,have
R1951 T8353 T8352 nmod montb222b,alleles
R1952 T8381 T8382 nummod 48,hpf
R1953 T8354 T8353 cc and,montb222b
R1954 T8355 T8353 conj montg234,montb222b
R1955 T8382 T8380 pobj hpf,by
R1956 T8383 T8368 punct ", ",has
R1957 T8384 T8368 prep in,has
R1958 T8385 T8384 pobj comparison,in
R1959 T8386 T8385 prep to,comparison
R1960 T8465 T8466 advmod In,vitro
R1961 T8466 T8467 amod vitro,experiments
R1962 T8387 T8388 det the,RBCs
R1963 T8388 T8386 pobj RBCs,to
R1964 T8467 T8469 nsubj experiments,demonstrate
R1965 T8468 T8467 compound translation,experiments
R1966 T8389 T8390 advmod approximately,"3,000"
R1967 T8470 T8467 prep in,experiments
R1968 T8471 T8472 compound reticulocyte,lysates
R1969 T8390 T8388 nummod "3,000",RBCs
R1970 T8472 T8470 pobj lysates,in
R1971 T8473 T8469 dobj reinitiation,demonstrate
R1972 T8474 T8473 prep of,reinitiation
R1973 T8391 T8388 prep in,RBCs
R1974 T8475 T8474 pobj translation,of
R1975 T8476 T8473 prep from,reinitiation
R1976 T8392 T8393 det the,circulation
R1977 T8477 T8478 det this,methionine
R1978 T8478 T8476 pobj methionine,from
R1979 T8393 T8391 pobj circulation,in
R1980 T8479 T8480 punct (,data
R1981 T8480 T8469 parataxis data,demonstrate
R1982 T8481 T8480 amod unpublished,data
R1983 T8394 T8393 prep of,circulation
R1984 T8482 T8480 punct ),data
R1985 T8483 T8469 punct .,demonstrate
R1986 T8395 T8396 amod wild,type
R1987 T8485 T8486 advmod Therefore,is
R1988 T8396 T8398 nmod type,embryos
R1989 T8487 T8486 punct ", ",is
R1990 T8488 T8489 det the,phenotype
R1991 T8489 T8486 nsubj phenotype,is
R1992 T8397 T8396 punct -,type
R1993 T8490 T8489 amod hypomorphic,phenotype
R1994 T8491 T8489 amod larval,phenotype
R1995 T8492 T8489 prep of,phenotype
R1996 T8398 T8394 pobj embryos,of
R1997 T8493 T8494 det the,allele
R1998 T8399 T8396 cc or,type
R1999 T8494 T8492 pobj allele,of
R2000 T8495 T8494 compound monm262,allele
R2001 T8400 T8396 conj heterozygous,type
R2002 T8496 T8486 advmod likely,is
R2003 T8497 T8486 prep due,is
R2004 T8498 T8497 pcomp to,due
R2005 T8401 T8385 prep at,comparison
R2006 T8499 T8500 amod partial,loss
R2007 T8500 T8497 pobj loss,due
R2008 T8501 T8500 prep of,loss
R2009 T8402 T8403 det the,point
R2010 T8502 T8503 compound mon,function
R2011 T8503 T8501 pobj function,of
R2012 T8504 T8503 cc or,function
R2013 T8403 T8401 pobj point,at
R2014 T8505 T8503 conj expression,function
R2015 T8506 T8486 punct .,is
R2016 T8404 T8403 amod same,point
R2017 T8508 T8509 det The,presence
R2018 T8509 T8510 nsubj presence,indicates
R2019 T8405 T8403 compound time,point
R2020 T8511 T8509 prep of,presence
R2021 T8512 T8511 pobj mutations,of
R2022 T8406 T8368 punct .,has
R2023 T8513 T8509 prep in,presence
R2024 T8514 T8513 pobj each,in
R2025 T8408 T8409 det The,allele
R2028 T8517 T8515 pobj alleles,of
R2029 T8518 T8517 compound mon,alleles
R2030 T8409 T8411 nsubjpass allele,found
R2031 T8519 T8520 mark that,is
R2032 T8520 T8510 ccomp is,indicates
R2033 T8521 T8522 amod defective,function
R2034 T8410 T8409 compound monm262,allele
R2035 T8522 T8520 nsubj function,is
R2036 T8523 T8522 compound Tif1γ,function
R2037 T8524 T8525 det the,cause
R2038 T8412 T8411 auxpass was,found
R2039 T8525 T8520 attr cause,is
R2040 T8526 T8525 prep of,cause
R2041 T8527 T8528 det the,phenotype
R2042 T8528 T8526 pobj phenotype,of
R2043 T8413 T8414 aux to,encode
R2044 T8529 T8528 compound mon,phenotype
R2045 T8530 T8510 punct .,indicates
R2046 T8414 T8411 xcomp encode,found
R2047 T8532 T8533 prep In,examined
R2048 T8534 T8532 pobj order,In
R2049 T8415 T8416 det a,codon
R2050 T8535 T8536 aux to,determine
R2051 T8536 T8534 acl determine,order
R2052 T8537 T8538 mark whether,expressed
R2053 T8538 T8536 ccomp expressed,determine
R2054 T8539 T8538 nsubjpass tif1γ,expressed
R2055 T8540 T8538 auxpass is,expressed
R2056 T8416 T8414 dobj codon,encode
R2057 T8541 T8538 prep in,expressed
R2058 T8542 T8543 amod hematopoietic,mesoderm
R2059 T8543 T8541 pobj mesoderm,in
R2060 T8417 T8416 amod premature,codon
R2061 T8544 T8533 punct ", ",examined
R2062 T8545 T8533 nsubj we,examined
R2063 T8546 T8533 advmod next,examined
R2064 T8418 T8416 compound stop,codon
R2065 T8547 T8548 compound zebrafish,embryos
R2066 T8548 T8533 dobj embryos,examined
R2067 T8549 T8533 prep by,examined
R2068 T8419 T8416 prep at,codon
R2069 T8550 T8551 amod whole,mount
R2070 T8551 T8553 nmod mount,hybridization
R2071 T8552 T8551 punct -,mount
R2072 T8420 T8421 compound position,E40
R2073 T8553 T8549 pobj hybridization,by
R2074 T8554 T8555 advmod in,situ
R2075 T8555 T8553 amod situ,hybridization
R2076 T8421 T8419 pobj E40,at
R2077 T8422 T8416 punct ", ",codon
R2078 T8423 T8424 dep which,encode
R2079 T8556 T8557 punct (,Figure
R2080 T8557 T8533 parataxis Figure,examined
R2081 T8424 T8416 relcl encode,codon
R2082 T8558 T8557 nummod 4A,Figure
R2083 T8559 T8557 punct ),Figure
R2084 T8425 T8424 aux would,encode
R2085 T8560 T8533 punct .,examined
R2086 T8562 T8563 compound tif1γ,mRNA
R2087 T8426 T8427 det a,protein
R2088 T8563 T8564 nsubjpass mRNA,expressed
R2089 T8427 T8424 dobj protein,encode
R2090 T8565 T8564 auxpass is,expressed
R2091 T8566 T8564 advmod maternally,expressed
R2092 T8428 T8427 amod putative,protein
R2093 T8567 T8564 cc and,expressed
R2094 T8568 T8569 auxpass is,found
R2095 T8569 T8564 conj found,expressed
R2096 T8429 T8427 prep of,protein
R2097 T8430 T8431 advmod only,acids
R2098 T8431 T8429 pobj acids,of
R2099 T8432 T8431 nummod 40,acids
R2100 T8570 T8569 prep throughout,found
R2101 T8571 T8572 det the,embryo
R2102 T8572 T8570 pobj embryo,throughout
R2103 T8573 T8569 prep during,found
R2104 T8574 T8575 compound blastula,stages
R2105 T8575 T8573 pobj stages,during
R2106 T8576 T8564 punct .,expressed
R2107 T8433 T8431 compound amino,acids
R2108 T8578 T8579 prep During,is
R2109 T8434 T8411 punct .,found
R2110 T8580 T8581 nmod gastrulation,stages
R2111 T8581 T8578 pobj stages,During
R2112 T8436 T8437 mark Although,expected
R2113 T8582 T8580 cc and,gastrulation
R2114 T8583 T8580 conj epiboly,gastrulation
R2115 T8584 T8579 punct ", ",is
R2116 T8585 T8586 amod zygotic,expression
R2117 T8437 T8442 advcl expected,found
R2118 T8586 T8579 nsubj expression,is
R2119 T8587 T8586 prep of,expression
R2120 T8588 T8587 pobj mon,of
R2121 T8438 T8439 det this,mutation
R2122 T8589 T8579 acomp highest,is
R2123 T8590 T8579 prep in,is
R2124 T8591 T8592 det the,mesendoderm
R2125 T8439 T8437 nsubjpass mutation,expected
R2126 T8592 T8590 pobj mesendoderm,in
R2127 T8593 T8592 prep of,mesendoderm
R2128 T8594 T8595 det the,ring
R2129 T8440 T8437 aux would,expected
R2130 T8595 T8593 pobj ring,of
R2131 T8596 T8595 compound germ,ring
R2132 T8597 T8579 punct .,is
R2133 T8441 T8437 auxpass be,expected
R2134 T8599 T8600 prep At,delineates
R2135 T8443 T8444 aux to,lead
R2136 T8601 T8602 nmod tail,bud
R2137 T8602 T8603 nmod bud,stages
R2138 T8603 T8599 pobj stages,At
R2139 T8444 T8437 xcomp lead,expected
R2140 T8604 T8602 cc and,bud
R2141 T8605 T8606 amod early,somite
R2142 T8606 T8602 conj somite,bud
R2143 T8445 T8444 prep to,lead
R2144 T8607 T8608 det a,level
R2145 T8608 T8600 nsubj level,delineates
R2146 T8609 T8608 amod high,level
R2147 T8446 T8447 det a,loss
R2148 T8610 T8608 prep of,level
R2149 T8611 T8612 compound tif1γ,expression
R2150 T8612 T8610 pobj expression,of
R2151 T8447 T8445 pobj loss,to
R2152 T8613 T8614 det a,population
R2153 T8614 T8600 dobj population,delineates
R2154 T8615 T8616 npadvmod horseshoe,shaped
R2155 T8616 T8614 amod shaped,population
R2156 T8617 T8616 punct -,shaped
R2157 T8618 T8614 prep of,population
R2158 T8619 T8620 amod ventral,lateral
R2159 T8448 T8447 amod complete,loss
R2160 T8620 T8622 amod lateral,mesoderm
R2161 T8621 T8620 punct /,lateral
R2162 T8622 T8618 pobj mesoderm,of
R2163 T8449 T8447 prep of,loss
R2164 T8623 T8624 dep that,give
R2165 T8624 T8614 relcl give,population
R2166 T8625 T8624 aux will,give
R2167 T8450 T8451 compound mon,gene
R2168 T8626 T8624 dobj rise,give
R2169 T8627 T8624 prep to,give
R2170 T8628 T8627 pobj blood,to
R2171 T8451 T8452 compound gene,product
R2172 T8629 T8600 cc and,delineates
R2173 T8630 T8631 advmod also,expresses
R2174 T8631 T8600 conj expresses,delineates
R2175 T8452 T8449 pobj product,of
R2176 T8632 T8633 nmod stem,cell
R2177 T8633 T8634 nmod cell,factor
R2178 T8634 T8631 dobj factor,expresses
R2179 T8453 T8442 punct ", ",found
R2180 T8635 T8634 amod leukemiahematopoietic,factor
R2181 T8636 T8634 compound transcription,factor
R2182 T8637 T8634 punct (,factor
R2183 T8454 T8455 det another,methionine
R2184 T8638 T8634 appos scl,factor
R2185 T8639 T8631 punct ),expresses
R2186 T8640 T8641 punct (,Liao
R2187 T8455 T8442 nsubjpass methionine,found
R2188 T8641 T8631 meta Liao,expresses
R2189 T8642 T8641 nmod et,Liao
R2190 T8456 T8442 auxpass is,found
R2191 T8457 T8442 advmod downstream,found
R2192 T8643 T8641 nmod al.,Liao
R2193 T8458 T8442 prep at,found
R2194 T8644 T8641 nummod 1997,Liao
R2195 T8645 T8641 punct ),Liao
R2196 T8646 T8600 punct .,delineates
R2197 T8459 T8460 compound amino,acid
R2198 T8648 T8649 det This,group
R2199 T8460 T8461 compound acid,position
R2200 T8649 T8650 nsubj group,continues
R2201 T8651 T8649 prep of,group
R2202 T8461 T8458 pobj position,at
R2203 T8652 T8651 pobj cells,of
R2204 T8653 T8654 aux to,express
R2205 T8654 T8650 xcomp express,continues
R2206 T8655 T8654 dobj tif1γ,express
R2207 T8656 T8655 cc and,tif1γ
R2208 T8657 T8655 conj scl,tif1γ
R2209 T8462 T8461 nummod 104,position
R2210 T8658 T8659 mark while,converges
R2211 T8659 T8654 advcl converges,express
R2212 T8660 T8659 nsubj it,converges
R2213 T8463 T8442 punct .,found
R2214 T8661 T8659 cc and,converges
R2215 T8662 T8659 conj forms,converges
R2216 T8663 T8664 det the,island
R2217 T8676 T8678 nsubjpass gene,expressed
R2218 T8664 T8662 dobj island,forms
R2219 T8665 T8664 amod embryonic,island
R2220 T8666 T8664 compound blood,island
R2221 T8667 T8668 punct (,Detrich
R2222 T8668 T8650 meta Detrich,continues
R2223 T8677 T8676 compound tif1γ,gene
R2224 T8669 T8668 nmod et,Detrich
R2225 T8670 T8668 nmod al.,Detrich
R2226 T8671 T8668 nummod 1995,Detrich
R2227 T8679 T8678 auxpass is,expressed
R2228 T8672 T8668 punct ),Detrich
R2229 T8673 T8650 punct .,continues
R2230 T8675 T8676 det The,gene
R2231 T8680 T8678 advmod also,expressed
R2232 T8681 T8678 advmod highly,expressed
R2233 T8682 T8678 prep in,expressed
R2234 T8683 T8684 det the,system
R2235 T8782 T8783 det the,sac
R2236 T8783 T8781 pobj sac,of
R2237 T8684 T8682 pobj system,in
R2238 T8784 T8783 compound yolk,sac
R2239 T8785 T8774 punct ", ",expressed
R2240 T8786 T8774 cc and,expressed
R2241 T8685 T8684 amod central,system
R2242 T8787 T8788 nsubjpass it,expressed
R2243 T8788 T8774 conj expressed,expressed
R2244 T8686 T8684 amod nervous,system
R2245 T8789 T8788 auxpass is,expressed
R2246 T8790 T8788 advmod subsequently,expressed
R2247 T8791 T8788 prep in,expressed
R2248 T8687 T8688 advmod as,as
R2249 T8792 T8793 det the,liver
R2250 T8793 T8791 pobj liver,in
R2251 T8794 T8793 amod fetal,liver
R2252 T8688 T8684 cc as,system
R2253 T8795 T8793 prep at,liver
R2254 T8796 T8797 det a,level
R2255 T8689 T8688 advmod well,as
R2256 T8797 T8795 pobj level,at
R2257 T8798 T8797 amod high,level
R2258 T8799 T8791 punct ", ",in
R2259 T8800 T8791 cc and,in
R2260 T8801 T8791 conj in,in
R2261 T8802 T8803 amod other,tissues
R2262 T8803 T8801 pobj tissues,in
R2263 T8804 T8803 punct ", ",tissues
R2264 T8690 T8691 det the,mesenchyme
R2265 T8805 T8803 prep including,tissues
R2266 T8806 T8807 det the,system
R2267 T8807 T8805 pobj system,including
R2268 T8691 T8684 conj mesenchyme,system
R2269 T8808 T8807 amod central,system
R2270 T8809 T8807 amod nervous,system
R2271 T8810 T8788 punct .,expressed
R2272 T8692 T8691 prep of,mesenchyme
R2273 T8812 T8813 advcl Taken,suggest
R2274 T8693 T8694 det the,trunk
R2275 T8814 T8812 advmod together,Taken
R2276 T8815 T8816 det these,results
R2277 T8816 T8813 nsubj results,suggest
R2278 T8694 T8692 pobj trunk,of
R2279 T8817 T8813 advmod strongly,suggest
R2280 T8818 T8819 mark that,are
R2281 T8695 T8694 cc and,trunk
R2282 T8696 T8694 conj tail,trunk
R2283 T8819 T8813 ccomp are,suggest
R2284 T8820 T8821 nmod zebrafish,mon
R2285 T8697 T8678 punct .,expressed
R2286 T8821 T8822 nmod mon,Tif1γ
R2287 T8822 T8819 nsubj Tif1γ,are
R2288 T8823 T8821 cc and,mon
R2289 T8699 T8700 amod Homozygous,mutants
R2290 T8824 T8821 conj mouse,mon
R2291 T8825 T8819 attr orthologs,are
R2292 T8826 T8827 dep that,function
R2293 T8827 T8825 relcl function,orthologs
R2294 T8700 T8702 nsubj mutants,have
R2295 T8828 T8827 prep during,function
R2296 T8829 T8828 pobj hematopoiesis,during
R2297 T8701 T8700 compound montg234,mutants
R2298 T8830 T8813 punct .,suggest
R2299 T8832 T8833 prep Given,searched
R2300 T8703 T8704 det a,amount
R2301 T8834 T8835 mark that,shown
R2302 T8835 T8832 pcomp shown,Given
R2303 T8704 T8702 dobj amount,have
R2304 T8836 T8837 amod mammalian,TIF1γ
R2305 T8837 T8835 nsubjpass TIF1γ,shown
R2306 T8838 T8835 aux has,shown
R2307 T8839 T8835 auxpass been,shown
R2308 T8705 T8706 advmod greatly,reduced
R2309 T8840 T8841 aux to,form
R2310 T8841 T8835 xcomp form,shown
R2311 T8842 T8841 dobj hetero-oligomers,form
R2312 T8843 T8841 prep with,form
R2313 T8844 T8843 pobj Tif1α,with
R2314 T8845 T8846 punct (,Peng
R2315 T8846 T8841 meta Peng,form
R2316 T8706 T8704 amod reduced,amount
R2317 T8847 T8846 nmod et,Peng
R2318 T8848 T8846 nmod al.,Peng
R2319 T8849 T8846 nummod 2002,Peng
R2320 T8707 T8704 prep of,amount
R2321 T8850 T8846 punct ),Peng
R2322 T8851 T8833 punct ", ",searched
R2323 T8852 T8833 nsubj we,searched
R2324 T8708 T8709 compound tif1γ,mRNA
R2325 T8853 T8833 prep for,searched
R2326 T8854 T8855 amod additional,members
R2327 T8855 T8853 pobj members,for
R2328 T8709 T8707 pobj mRNA,of
R2329 T8856 T8855 compound TIF1,members
R2330 T8857 T8855 compound family,members
R2331 T8858 T8855 prep in,members
R2332 T8710 T8702 prep in,have
R2333 T8859 T8858 pobj zebrafish,in
R2334 T8860 T8861 aux to,compare
R2335 T8861 T8833 advcl compare,searched
R2336 T8711 T8712 det all,tissues
R2337 T8862 T8861 prep with,compare
R2338 T8863 T8862 pobj tif1γ,with
R2339 T8864 T8833 punct .,searched
R2340 T8712 T8710 pobj tissues,in
R2341 T8866 T8867 advcl Using,designed
R2342 T8713 T8702 advcl consistent,have
R2343 T8868 T8869 nmod zebrafish,sequences
R2344 T8869 T8866 dobj sequences,Using
R2345 T8714 T8713 prep with,consistent
R2346 T8870 T8871 nmod expressed,tag
R2347 T8871 T8869 nmod tag,sequences
R2348 T8872 T8871 nmod sequence,tag
R2349 T8715 T8716 npadvmod nonsense,mediated
R2350 T8873 T8871 punct (,tag
R2351 T8874 T8871 appos EST,tag
R2352 T8875 T8869 punct ),sequences
R2353 T8716 T8718 amod mediated,decay
R2354 T8876 T8867 punct ", ",designed
R2355 T8877 T8867 nsubj we,designed
R2356 T8878 T8867 dobj primers,designed
R2357 T8717 T8716 punct -,mediated
R2358 T8879 T8880 aux to,amplify
R2359 T8880 T8867 advcl amplify,designed
R2360 T8881 T8880 dep RT,amplify
R2361 T8882 T8880 punct -,amplify
R2362 T8883 T8880 dep PCR,amplify
R2363 T8884 T8885 det a,cDNA
R2364 T8885 T8880 dobj cDNA,amplify
R2365 T8718 T8714 pobj decay,with
R2366 T8886 T8885 nmod TIF1,cDNA
R2367 T8887 T8886 punct -,TIF1
R2368 T8719 T8718 compound message,decay
R2369 T8720 T8702 punct .,have
R2370 T8722 T8723 advmod Thus,expressed
R2371 T8724 T8723 punct ", ",expressed
R2373 T8725 T8726 compound zebrafish,tif1γ
R2376 T8726 T8723 nsubjpass tif1γ,expressed
R2377 T8891 T8889 pobj RNA,from
R2378 T8892 T8893 nummod 10,hpf
R2379 T8893 T8891 nmod hpf,RNA
R2380 T8727 T8723 auxpass is,expressed
R2381 T8894 T8893 punct -,hpf
R2382 T8895 T8893 cc and,hpf
R2383 T8728 T8723 advmod specifically,expressed
R2384 T8896 T8897 nummod 24,hpf
R2385 T8897 T8893 conj hpf,hpf
R2386 T8898 T8897 punct -,hpf
R2387 T8729 T8723 prep in,expressed
R2388 T8899 T8867 punct .,designed
R2389 T8730 T8731 amod ventral,mesoderm
R2390 T8901 T8902 det This,cDNA
R2391 T8902 T8903 nsubj cDNA,encodes
R2392 T8731 T8729 pobj mesoderm,in
R2393 T8904 T8905 det a,ortholog
R2394 T8732 T8731 cc and,mesoderm
R2395 T8905 T8903 dobj ortholog,encodes
R2396 T8906 T8905 amod predicted,ortholog
R2397 T8907 T8905 compound zebrafish,ortholog
R2398 T8733 T8734 amod putative,hemangioblasts
R2399 T8908 T8905 prep of,ortholog
R2400 T8909 T8910 amod human,TIF1α
R2401 T8734 T8731 conj hemangioblasts,mesoderm
R2402 T8910 T8908 pobj TIF1α,of
R2403 T8911 T8903 prep based,encodes
R2404 T8912 T8911 prep on,based
R2405 T8735 T8723 prep prior,expressed
R2406 T8913 T8914 amod predicted,sequences
R2407 T8914 T8912 pobj sequences,on
R2408 T8736 T8735 prep to,prior
R2409 T8915 T8916 compound amino,acid
R2410 T8916 T8914 compound acid,sequences
R2411 T8737 T8735 cc and,prior
R2412 T8917 T8918 punct (,see
R2413 T8918 T8903 parataxis see,encodes
R2414 T8919 T8918 dobj Figure,see
R2415 T8920 T8919 nummod 3B,Figure
R2416 T8921 T8918 punct ),see
R2417 T8922 T8903 punct .,encodes
R2418 T8738 T8735 conj during,prior
R2419 T8924 T8925 prep In,map
R2420 T8739 T8740 det the,stages
R2421 T8926 T8924 pobj addition,In
R2422 T8927 T8925 punct ", ",map
R2423 T8928 T8929 compound zebrafish,ESTs
R2424 T8740 T8738 pobj stages,during
R2425 T8929 T8925 nsubj ESTs,map
R2426 T8930 T8929 compound tif1α,ESTs
R2427 T8931 T8925 prep to,map
R2428 T8741 T8740 amod embryonic,stages
R2429 T8932 T8931 pobj LG4,to
R2430 T8933 T8925 prep in,map
R2431 T8934 T8935 det a,region
R2432 T8742 T8743 advmod when,undergoing
R2433 T8935 T8933 pobj region,in
R2434 T8936 T8935 acl predicted,region
R2435 T8937 T8938 aux to,be
R2436 T8743 T8740 relcl undergoing,stages
R2437 T8938 T8936 xcomp be,predicted
R2438 T8939 T8938 acomp syntenic,be
R2439 T8940 T8939 prep to,syntenic
R2440 T8744 T8745 amod hematopoietic,progenitors
R2441 T8941 T8942 det the,region
R2442 T8942 T8940 pobj region,to
R2443 T8943 T8942 prep of,region
R2444 T8745 T8743 nsubj progenitors,undergoing
R2445 T8944 T8945 amod human,Chromosome
R2446 T8945 T8943 pobj Chromosome,of
R2447 T8946 T8945 nummod 7,Chromosome
R2448 T8746 T8743 aux are,undergoing
R2449 T8947 T8948 dep that,contains
R2450 T8948 T8945 relcl contains,Chromosome
R2451 T8949 T8950 det the,gene
R2452 T8747 T8743 dobj apoptosis,undergoing
R2453 T8950 T8948 dobj gene,contains
R2454 T8951 T8950 compound TIF1α,gene
R2455 T8952 T8936 prep based,predicted
R2456 T8748 T8743 prep in,undergoing
R2457 T8953 T8952 prep on,based
R2458 T8954 T8955 det the,locations
R2459 T8955 T8953 pobj locations,on
R2460 T8749 T8750 compound mon,mutants
R2461 T8956 T8955 amod conserved,locations
R2462 T8957 T8955 prep of,locations
R2463 T8958 T8959 nummod eight,pairs
R2464 T8750 T8748 pobj mutants,in
R2465 T8959 T8957 pobj pairs,of
R2466 T8960 T8959 amod other,pairs
R2467 T8961 T8959 amod orthologous,pairs
R2468 T8962 T8959 compound gene,pairs
R2469 T8963 T8955 punct ", ",locations
R2470 T8751 T8723 punct .,expressed
R2471 T8964 T8955 prep including,locations
R2472 T8965 T8964 pobj SEMA3A,including
R2473 T8966 T8955 punct ", ",locations
R2474 T8753 T8754 nsubj We,compared
R2475 T8967 T8955 acl mapped,locations
R2476 T8968 T8967 prep to,mapped
R2477 T8969 T8970 det these,regions
R2478 T8970 T8968 pobj regions,to
R2479 T8755 T8754 advmod also,compared
R2480 T8971 T8970 prep in,regions
R2481 T8972 T8973 amod human,zebrafish
R2482 T8973 T8971 pobj zebrafish,in
R2483 T8974 T8973 cc and,zebrafish
R2484 T8756 T8757 det the,expression
R2485 T8975 T8976 punct (,Barbazuk
R2486 T8976 T8925 meta Barbazuk,map
R2487 T8977 T8976 nmod et,Barbazuk
R2488 T8757 T8754 dobj expression,compared
R2489 T8978 T8976 nmod al.,Barbazuk
R2490 T8979 T8976 nummod 2000,Barbazuk
R2491 T8980 T8976 punct ),Barbazuk
R2492 T8758 T8757 prep of,expression
R2493 T8981 T8925 punct .,map
R2494 T8983 T8984 nsubj We,compared
R2495 T8759 T8760 compound zebrafish,mon
R2496 T8985 T8984 advmod next,compared
R2497 T8986 T8987 det the,pattern
R2498 T8760 T8758 pobj mon,of
R2499 T8987 T8984 dobj pattern,compared
R2500 T8988 T8987 amod embryonic,pattern
R2501 T8989 T8987 compound expression,pattern
R2502 T8761 T8754 prep to,compared
R2503 T8990 T8987 prep of,pattern
R2504 T8762 T8763 compound mouse,Tif1γ
R2505 T8763 T8761 pobj Tif1γ,to
R2506 T8991 T8992 compound tif1α,mRNA
R2507 T8992 T8990 pobj mRNA,of
R2508 T8993 T8984 prep to,compared
R2509 T8764 T8765 punct (,4A
R2510 T8765 T8754 parataxis 4A,compared
R2511 T8766 T8765 nmod Figure,4A
R2512 T8767 T8765 cc and,4A
R2513 T8994 T8993 pobj tif1γ,to
R2514 T8995 T8984 prep by,compared
R2515 T8768 T8765 conj 4B,4A
R2516 T8996 T8997 advmod in,situ
R2517 T8997 T8998 amod situ,hybridization
R2518 T8998 T8995 pobj hybridization,by
R2519 T8999 T8984 punct .,compared
R2520 T8769 T8765 punct ),4A
R2521 T9001 T9002 prep Like,expressed
R2522 T8770 T8754 punct .,compared
R2523 T9003 T9004 amod mammalian,TIF1α
R2524 T9004 T9001 pobj TIF1α,Like
R2525 T9005 T9006 punct (,Le
R2526 T8772 T8773 compound Mouse,Tif1γ
R2527 T9006 T9004 meta Le,TIF1α
R2528 T9007 T9006 nmod Douarin,Le
R2529 T9008 T9006 nmod et,Le
R2530 T9009 T9006 nmod al.,Le
R2531 T8773 T8774 nsubjpass Tif1γ,expressed
R2532 T9010 T9006 nummod 1995,Le
R2533 T9011 T9006 punct ;,Le
R2534 T9012 T9006 nmod Niederreither,Le
R2535 T8775 T8774 auxpass is,expressed
R2536 T9013 T9006 nmod et,Le
R2537 T9014 T9006 nmod al.,Le
R2538 T9015 T9006 nummod 1999,Le
R2539 T9016 T9006 punct ),Le
R2540 T8776 T8774 advmod highly,expressed
R2541 T9017 T9002 punct ", ",expressed
R2542 T9018 T9019 det the,gene
R2543 T9019 T9002 nsubjpass gene,expressed
R2544 T8777 T8774 prep in,expressed
R2545 T9020 T9019 amod predicted,gene
R2546 T9021 T9019 compound zebrafish,gene
R2547 T9022 T9019 compound tif1γ,gene
R2548 T8778 T8779 amod erythroid,islands
R2549 T9023 T9002 auxpass is,expressed
R2550 T9024 T9002 advmod broadly,expressed
R2551 T9025 T9026 punct (,see
R2552 T8779 T8777 pobj islands,in
R2553 T9026 T9002 parataxis see,expressed
R2554 T9027 T9026 dobj Figure,see
R2555 T9028 T9027 nummod 4A,Figure
R2556 T8780 T8779 compound blood,islands
R2557 T9029 T9026 punct ),see
R2558 T9030 T9002 punct .,expressed
R2559 T8781 T8779 prep of,islands
R2560 T9032 T9033 prep At,display
R2561 T9034 T9035 nummod five,somites
R2562 T9100 T9097 advmod vivo,form
R2563 T9035 T9032 pobj somites,At
R2564 T9036 T9033 punct ", ",display
R2565 T9037 T9038 compound zebrafish,tif1α
R2566 T9038 T9033 nsubj tif1α,display
R2567 T9101 T9081 punct .,coexpressed
R2568 T9039 T9033 aux does,display
R2569 T9040 T9033 neg not,display
R2570 T9041 T9042 det the,expression
R2571 T9042 T9033 dobj expression,display
R2572 T9043 T9044 advmod relatively,high
R2573 T9044 T9042 amod high,expression
R2574 T9045 T9033 prep in,display
R2575 T9046 T9047 det the,region
R2576 T9047 T9045 pobj region,in
R2577 T9048 T9047 nmod horseshoe,region
R2578 T9049 T9048 punct -,horseshoe
R2579 T9050 T9048 amod shaped,horseshoe
R2580 T9051 T9047 prep of,region
R2581 T9052 T9053 amod hematopoietic,mesoderm
R2582 T9053 T9051 pobj mesoderm,of
R2583 T9054 T9053 acl seen,mesoderm
R2584 T9055 T9054 prep with,seen
R2585 T9056 T9055 pobj tif1γ,with
R2586 T9057 T9033 punct .,display
R2587 T9059 T9060 prep At,expressed
R2588 T9061 T9062 amod later,stages
R2589 T9062 T9059 pobj stages,At
R2590 T9063 T9060 punct ", ",expressed
R2591 T9064 T9060 nsubjpass tif1α,expressed
R2592 T9065 T9060 auxpass is,expressed
R2593 T9066 T9060 advmod evenly,expressed
R2594 T9067 T9060 prep throughout,expressed
R2595 T9068 T9067 pobj most,throughout
R2596 T9069 T9068 prep of,most
R2597 T9070 T9071 det the,embryo
R2598 T9071 T9069 pobj embryo,of
R2599 T9072 T9068 punct ", ",most
R2600 T9073 T9068 prep including,most
R2601 T9074 T9075 det the,islands
R2602 T9075 T9073 pobj islands,including
R2603 T9076 T9075 amod developing,islands
R2604 T9077 T9075 compound blood,islands
R2605 T9078 T9060 punct .,expressed
R2606 T9080 T9081 advmod Therefore,coexpressed
R2607 T9082 T9081 punct ", ",coexpressed
R2608 T9083 T9081 nsubjpass tif1α,coexpressed
R2609 T9084 T9081 auxpass is,coexpressed
R2610 T9085 T9081 prep in,coexpressed
R2611 T9086 T9087 det the,cells
R2612 T9087 T9085 pobj cells,in
R2613 T9088 T9087 amod same,cells
R2614 T9089 T9081 prep with,coexpressed
R2615 T9090 T9089 pobj tif1γ,with
R2616 T9091 T9081 cc and,coexpressed
R2617 T9092 T9093 aux may,be
R2618 T9093 T9081 conj be,coexpressed
R2619 T9094 T9093 advmod therefore,be
R2620 T9095 T9093 acomp available,be
R2621 T9096 T9097 aux to,form
R2622 T9097 T9093 advcl form,be
R2623 T9098 T9097 dobj hetero-oligomers,form
R2624 T9099 T9100 advmod in,vivo
R2629 T9620 T9621 amod Forced,Expression
R2630 T9622 T9621 prep of,Expression
R2631 T9623 T9624 nmod tif1γ,Hematopoiesis
R2632 T9624 T9622 pobj Hematopoiesis,of
R2633 T9625 T9624 nmod Rescues,Hematopoiesis
R2634 T9626 T9621 prep in,Expression
R2635 T9627 T9628 compound mon,Mutants
R2636 T9628 T9626 pobj Mutants,in
R2637 T9630 T9631 aux To,confirm
R2638 T9631 T9633 advcl confirm,performed
R2639 T9632 T9631 advmod further,confirm
R2640 T9634 T9635 mark that,is
R2641 T9635 T9631 ccomp is,confirm
R2642 T9636 T9637 det a,mutation
R2643 T9637 T9635 nsubj mutation,is
R2644 T9638 T9637 prep in,mutation
R2645 T9639 T9640 det the,gene
R2646 T9640 T9638 pobj gene,in
R2647 T9641 T9640 compound zebrafish,gene
R2648 T9642 T9640 compound tif1γ,gene
R2649 T9643 T9635 acomp responsible,is
R2650 T9644 T9643 prep for,responsible
R2651 T9645 T9646 det the,phenotype
R2652 T9646 T9644 pobj phenotype,for
R2653 T9647 T9648 npadvmod mon,mutant
R2654 T9648 T9646 amod mutant,phenotype
R2655 T9649 T9633 nsubj we,performed
R2656 T9650 T9651 compound embryo,experiments
R2657 T9651 T9633 dobj experiments,performed
R2658 T9652 T9651 compound rescue,experiments
R2659 T9653 T9654 punct (,Table
R2660 T9654 T9633 parataxis Table,performed
R2661 T9655 T9654 dep Figure,Table
R2662 T9656 T9655 nummod 5A,Figure
R2663 T9657 T9654 punct ;,Table
R2664 T9658 T9654 nummod 1,Table
R2665 T9659 T9654 punct ),Table
R2666 T9660 T9633 punct .,performed
R2667 T9662 T9663 nsubj Microinjection,rescues
R2668 T9664 T9662 prep of,Microinjection
R2669 T9665 T9666 amod synthetic,mRNA
R2670 T9666 T9664 pobj mRNA,of
R2671 T9667 T9668 amod wild,type
R2672 T9668 T9666 compound type,mRNA
R2673 T9669 T9668 punct -,type
R2674 T9670 T9666 compound mon,mRNA
R2675 T9671 T9662 prep at,Microinjection
R2676 T9672 T9673 det the,stage
R2677 T9673 T9671 pobj stage,at
R2678 T9674 T9675 nummod one,cell
R2679 T9675 T9673 compound cell,stage
R2680 T9676 T9675 punct -,cell
R2681 T9677 T9678 det the,formation
R2682 T9678 T9663 dobj formation,rescues
R2683 T9679 T9678 prep of,formation
R2684 T9680 T9681 amod embryonic,erythrocytes
R2685 T9681 T9679 pobj erythrocytes,of
R2686 T9682 T9663 prep in,rescues
R2687 T9683 T9684 amod genotyped,embryos
R2688 T9684 T9682 pobj embryos,in
R2689 T9685 T9684 amod mutant,embryos
R2690 T9686 T9663 prep without,rescues
R2691 T9687 T9686 pcomp causing,without
R2692 T9688 T9689 amod obvious,defects
R2693 T9689 T9687 dobj defects,causing
R2694 T9690 T9689 prep in,defects
R2695 T9691 T9692 amod embryonic,patterning
R2696 T9692 T9690 pobj patterning,in
R2697 T9693 T9692 cc or,patterning
R2698 T9694 T9692 conj organogenesis,patterning
R2699 T9695 T9663 punct .,rescues
R2700 T9697 T9698 prep At,show
R2701 T9699 T9700 nummod 4,d
R2702 T9700 T9697 pobj d,At
R2703 T9701 T9700 prep of,d
R2704 T9702 T9701 pobj development,of
R2705 T9703 T9698 punct ", ",show
R2706 T9704 T9705 nummod 70,%
R2707 T9705 T9698 nsubj %,show
R2708 T9706 T9707 punct (,10
R2709 T9707 T9705 parataxis 10,%
R2710 T9708 T9707 nsubj n,10
R2711 T9709 T9707 punct =,10
R2712 T9710 T9707 punct ),10
R2713 T9711 T9705 prep of,%
R2714 T9712 T9713 compound montg234,mutants
R2715 T9713 T9711 pobj mutants,of
R2716 T9714 T9715 amod significant,rescue
R2717 T9715 T9698 dobj rescue,show
R2718 T9716 T9717 punct (,cells
R2719 T9717 T9715 parataxis cells,rescue
R2720 T9718 T9719 amod greater,200
R2721 T9719 T9717 nummod 200,cells
R2722 T9720 T9719 quantmod than,200
R2723 T9721 T9717 prep in,cells
R2724 T9722 T9721 pobj comparison,in
R2725 T9723 T9722 prep to,comparison
R2726 T9724 T9725 det a,estimate
R2727 T9725 T9723 pobj estimate,to
R2728 T9726 T9727 amod wild,type
R2729 T9727 T9725 compound type,estimate
R2730 T9728 T9727 punct -,type
R2731 T9729 T9725 prep of,estimate
R2732 T9730 T9731 nummod "3,000",cells
R2733 T9731 T9729 pobj cells,of
R2734 T9732 T9717 punct ),cells
R2735 T9733 T9715 prep of,rescue
R2736 T9734 T9735 amod circulating,RBCs
R2737 T9735 T9733 pobj RBCs,of
R2738 T9736 T9735 amod hemoglobinized,RBCs
R2739 T9737 T9698 prep in,show
R2740 T9738 T9737 pobj comparison,in
R2741 T9739 T9738 prep to,comparison
R2742 T9740 T9741 compound control,mutants
R2743 T9741 T9739 pobj mutants,to
R2744 T9742 T9741 compound sibling,mutants
R2745 T9743 T9744 punct (,75
R2746 T9744 T9698 parataxis 75,show
R2747 T9745 T9744 nsubj n,75
R2748 T9746 T9744 punct =,75
R2749 T9747 T9744 punct ),75
R2750 T9748 T9698 punct .,show
R2751 T9750 T9751 prep Based,rescued
R2752 T9752 T9750 prep on,Based
R2753 T9753 T9754 det the,correction
R2754 T9754 T9752 pobj correction,on
R2755 T9755 T9754 prep of,correction
R2756 T9756 T9757 det the,phenotype
R2757 T9757 T9755 pobj phenotype,of
R2758 T9758 T9757 amod jagged,phenotype
R2759 T9759 T9760 compound fin,fold
R2760 T9760 T9757 compound fold,phenotype
R2761 T9761 T9760 punct -,fold
R2762 T9762 T9763 punct (,Ransom
R2763 T9763 T9754 meta Ransom,correction
R2764 T9764 T9763 nmod et,Ransom
R2765 T9765 T9763 nmod al.,Ransom
R2766 T9766 T9763 nummod 1996,Ransom
R2767 T9767 T9763 punct ),Ransom
R2768 T9768 T9751 punct ", ",rescued
R2769 T9769 T9770 det the,cells
R2770 T9770 T9751 nsubjpass cells,rescued
R2771 T9771 T9770 amod mesenchymal,cells
R2772 T9772 T9751 auxpass are,rescued
R2773 T9773 T9751 prep to,rescued
R2774 T9774 T9775 det a,extent
R2775 T9775 T9773 pobj extent,to
R2776 T9776 T9775 amod similar,extent
R2777 T9777 T9775 prep as,extent
R2778 T9778 T9779 det the,anemia
R2779 T9779 T9777 pobj anemia,as
R2780 T9780 T9781 punct (,data
R2781 T9781 T9751 parataxis data,rescued
R2782 T9782 T9781 amod unpublished,data
R2783 T9783 T9781 punct ),data
R2784 T9784 T9751 punct .,rescued
R2785 T9786 T9787 nsubj Overexpression,result
R2786 T9788 T9786 prep of,Overexpression
R2787 T9789 T9788 pobj mon,of
R2788 T9790 T9787 aux did,result
R2789 T9791 T9787 neg not,result
R2790 T9792 T9787 prep in,result
R2791 T9793 T9794 amod expanded,numbers
R2792 T9794 T9792 pobj numbers,in
R2793 T9795 T9794 compound blood,numbers
R2794 T9796 T9794 compound cell,numbers
R2795 T9797 T9787 prep in,result
R2796 T9798 T9799 amod wild,type
R2797 T9799 T9801 compound type,embryos
R2798 T9800 T9799 punct -,type
R2799 T9801 T9797 pobj embryos,in
R2800 T9802 T9787 cc and,result
R2801 T9803 T9787 conj was,result
R2802 T9804 T9803 neg not,was
R2803 T9805 T9803 acomp toxic,was
R2804 T9806 T9803 prep at,was
R2805 T9807 T9806 pobj doses,at
R2806 T9808 T9809 dep that,rescue
R2807 T9809 T9807 relcl rescue,doses
R2808 T9810 T9811 det the,phenotype
R2809 T9811 T9809 dobj phenotype,rescue
R2810 T9812 T9811 prep of,phenotype
R2811 T9813 T9814 compound mon,mutants
R2812 T9814 T9812 pobj mutants,of
R2813 T9815 T9816 punct (,data
R2814 T9816 T9803 parataxis data,was
R2815 T9817 T9816 amod unpublished,data
R2816 T9818 T9816 punct ),data
R2817 T9819 T9787 punct .,result
R2818 T9821 T9822 mark Since,were
R2819 T9822 T9824 advcl were,suggest
R2820 T9823 T9822 expl there,were
R2821 T9825 T9826 det no,populations
R2822 T9826 T9822 attr populations,were
R2823 T9827 T9826 amod expanded,populations
R2824 T9828 T9827 cc or,expanded
R2825 T9829 T9827 conj ectopic,expanded
R2826 T9830 T9826 compound blood,populations
R2827 T9831 T9822 prep in,were
R2828 T9832 T9833 det the,embryos
R2829 T9833 T9831 pobj embryos,in
R2830 T9834 T9824 punct ", ",suggest
R2831 T9835 T9836 det these,experiments
R2832 T9836 T9824 nsubj experiments,suggest
R2833 T9837 T9836 compound rescue,experiments
R2834 T9838 T9839 mark that,functions
R2835 T9839 T9824 ccomp functions,suggest
R2836 T9840 T9839 nsubj mon,functions
R2837 T9841 T9839 prep as,functions
R2838 T9842 T9843 det a,factor
R2839 T9843 T9841 pobj factor,as
R2840 T9844 T9843 amod permissive,factor
R2841 T9845 T9843 acl required,factor
R2842 T9846 T9845 prep for,required
R2843 T9847 T9846 pobj hematopoiesis,for
R2844 T9848 T9824 punct .,suggest
R2847 T10666 T10667 compound Marrow,Transplantation
R2848 T10667 T10668 nsubj Transplantation,Rescues
R2849 T10669 T10668 dobj Erythropoiesis,Rescues
R2850 T10670 T10668 prep in,Rescues
R2851 T10671 T10672 compound mon,Mutants
R2852 T10672 T10670 pobj Mutants,in
R2853 T10674 T10675 det The,levels
R2854 T10675 T10677 nsubj levels,suggest
R2855 T10676 T10675 amod high,levels
R2856 T10678 T10675 prep of,levels
R2857 T10679 T10680 compound tif1γ,expression
R2858 T10680 T10678 pobj expression,of
R2859 T10681 T10675 prep in,levels
R2860 T10682 T10683 amod erythroid,cells
R2861 T10683 T10681 pobj cells,in
R2862 T10684 T10685 mark that,functions
R2863 T10685 T10677 ccomp functions,suggest
R2864 T10686 T10685 nsubj it,functions
R2865 T10687 T10685 prep as,functions
R2866 T10688 T10689 det a,regulator
R2867 T10689 T10687 pobj regulator,as
R2868 T10690 T10691 npadvmod cell,autonomous
R2869 T10691 T10689 amod autonomous,regulator
R2870 T10692 T10691 punct -,autonomous
R2871 T10693 T10689 prep of,regulator
R2872 T10694 T10695 compound gene,expression
R2873 T10695 T10693 pobj expression,of
R2874 T10696 T10685 prep in,functions
R2875 T10697 T10698 amod hematopoietic,cells
R2876 T10698 T10696 pobj cells,in
R2877 T10699 T10677 punct .,suggest
R2878 T10701 T10702 prep In,transplanted
R2879 T10703 T10701 pobj order,In
R2880 T10704 T10705 aux to,test
R2881 T10705 T10703 acl test,order
R2882 T10706 T10707 det this,hypothesis
R2883 T10707 T10705 dobj hypothesis,test
R2884 T10708 T10702 punct ", ",transplanted
R2885 T10709 T10702 nsubj we,transplanted
R2886 T10710 T10711 amod wild,type
R2887 T10711 T10713 nmod type,cells
R2888 T10712 T10711 punct -,type
R2889 T10713 T10702 dobj cells,transplanted
R2890 T10714 T10713 amod adult,cells
R2891 T10715 T10713 compound zebrafish,cells
R2892 T10716 T10713 compound kidney,cells
R2893 T10717 T10713 compound marrow,cells
R2894 T10718 T10713 acl carrying,cells
R2895 T10719 T10720 det a,transgene
R2896 T10720 T10718 dobj transgene,carrying
R2897 T10721 T10720 nmod gata1,transgene
R2898 T10722 T10721 punct :,gata1
R2899 T10723 T10724 amod green,protein
R2900 T10724 T10721 appos protein,gata1
R2901 T10725 T10724 amod fluorescent,protein
R2902 T10726 T10724 punct (,protein
R2903 T10727 T10724 appos GFP,protein
R2904 T10728 T10720 punct ),transgene
R2905 T10729 T10702 prep into,transplanted
R2906 T10730 T10731 nummod 48,hpf
R2907 T10731 T10733 nmod hpf,embryos
R2908 T10732 T10731 punct -,hpf
R2909 T10733 T10729 pobj embryos,into
R2910 T10734 T10735 npadvmod mon,mutant
R2911 T10735 T10733 amod mutant,embryos
R2912 T10736 T10737 punct (,Table
R2913 T10737 T10702 parataxis Table,transplanted
R2914 T10738 T10737 dep Figure,Table
R2915 T10739 T10738 nummod 5B,Figure
R2916 T10740 T10737 punct ;,Table
R2917 T10741 T10737 nummod 2,Table
R2918 T10742 T10737 punct ),Table
R2919 T10743 T10702 punct .,transplanted
R2920 T10745 T10746 det The,transgene
R2921 T10746 T10750 nsubj transgene,makes
R2922 T10747 T10748 nmod gata1,GFP
R2923 T10748 T10746 compound GFP,transgene
R2924 T10749 T10748 punct :,GFP
R2925 T10751 T10750 dobj use,makes
R2926 T10752 T10750 prep of,makes
R2927 T10753 T10754 det the,promoter
R2928 T10754 T10752 pobj promoter,of
R2929 T10755 T10754 compound gata1,promoter
R2930 T10756 T10757 aux to,drive
R2931 T10757 T10750 advcl drive,makes
R2932 T10758 T10759 compound GFP,expression
R2933 T10759 T10757 dobj expression,drive
R2934 T10760 T10750 cc and,makes
R2935 T10761 T10762 aux can,used
R2936 T10762 T10750 conj used,makes
R2937 T10763 T10762 advmod thus,used
R2938 T10764 T10762 auxpass be,used
R2939 T10765 T10766 aux to,mark
R2940 T10766 T10762 advcl mark,used
R2941 T10767 T10768 npadvmod donor,derived
R2942 T10768 T10770 amod derived,cells
R2943 T10769 T10768 punct -,derived
R2944 T10770 T10766 dobj cells,mark
R2945 T10771 T10770 amod erythroid,cells
R2946 T10772 T10773 punct (,Long
R2947 T10773 T10762 meta Long,used
R2948 T10774 T10773 nmod et,Long
R2949 T10775 T10773 nmod al.,Long
R2950 T10776 T10773 nummod 1997,Long
R2951 T10777 T10773 punct ),Long
R2952 T10778 T10750 punct .,makes
R2953 T10780 T10781 amod Untransplanted,embryos
R2954 T10781 T10783 nsubj embryos,have
R2955 T10782 T10781 amod mutant,embryos
R2956 T10784 T10785 det no,cells
R2957 T10785 T10783 dobj cells,have
R2958 T10786 T10785 amod embryonic,cells
R2959 T10787 T10785 compound blood,cells
R2960 T10788 T10783 prep in,have
R2961 T10789 T10788 pobj circulation,in
R2962 T10790 T10783 punct .,have
R2963 T10792 T10793 prep Following,observed
R2964 T10794 T10792 pobj transplantation,Following
R2965 T10795 T10793 punct ", ",observed
R2966 T10796 T10797 amod mutant,embryos
R2967 T10797 T10793 nsubjpass embryos,observed
R2968 T10798 T10797 compound host,embryos
R2969 T10799 T10793 auxpass were,observed
R2970 T10800 T10793 advmod daily,observed
R2971 T10801 T10793 prep for,observed
R2972 T10802 T10803 nummod 2,wk
R2973 T10803 T10801 pobj wk,for
R2974 T10804 T10793 punct .,observed
R2975 T10806 T10807 prep Of,showed
R2976 T10808 T10809 nummod 191,embryos
R2977 T10809 T10806 pobj embryos,Of
R2978 T10810 T10809 amod mutant,embryos
R2979 T10811 T10809 acl injected,embryos
R2980 T10812 T10807 punct ", ",showed
R2981 T10813 T10807 nsubj 129,showed
R2982 T10814 T10815 punct (,%
R2983 T10815 T10813 parataxis %,129
R2984 T10816 T10815 nummod 68,%
R2985 T10817 T10815 punct ),%
R2986 T10818 T10819 nmod GFP,cells
R2987 T10819 T10807 dobj cells,showed
R2988 T10820 T10818 punct +,GFP
R2989 T10821 T10807 prep in,showed
R2990 T10822 T10821 pobj circulation,in
R2991 T10823 T10824 nummod 2,d
R2992 T10824 T10825 npadvmod d,later
R2993 T10825 T10807 advmod later,showed
R2994 T10826 T10807 punct .,showed
R2995 T10828 T10829 amod Many,recipients
R2996 T10829 T10830 nsubj recipients,showed
R2997 T10831 T10832 amod robust,increases
R2998 T10832 T10830 dobj increases,showed
R2999 T10833 T10830 prep in,showed
R3000 T10834 T10835 nmod donor,cells
R3001 T10835 T10833 pobj cells,in
R3002 T10836 T10834 punct -,donor
R3003 T10837 T10834 amod derived,donor
R3004 T10838 T10830 prep over,showed
R3005 T10839 T10840 det the,period
R3006 T10840 T10838 pobj period,over
R3007 T10841 T10840 compound observation,period
R3008 T10842 T10830 punct .,showed
R3009 T10844 T10845 prep Of,demonstrated
R3010 T10846 T10847 nummod 81,recipients
R3011 T10847 T10844 pobj recipients,Of
R3012 T10848 T10849 advmod initially,scored
R3013 T10849 T10847 acl scored,recipients
R3014 T10850 T10849 prep as,scored
R3015 T10851 T10850 pcomp having,as
R3016 T10852 T10853 amod less,ten
R3017 T10853 T10855 nummod ten,cells
R3018 T10854 T10853 quantmod than,ten
R3019 T10855 T10851 dobj cells,having
R3020 T10856 T10855 nmod GFP,cells
R3021 T10857 T10856 punct +,GFP
R3022 T10858 T10851 prep at,having
R3023 T10859 T10858 pobj day,at
R3024 T10860 T10859 nummod 2,day
R3025 T10861 T10859 advmod posttransplant,day
R3026 T10862 T10845 punct ", ",demonstrated
R3027 T10863 T10845 nsubj 13,demonstrated
R3028 T10864 T10865 punct (,%
R3029 T10865 T10863 parataxis %,13
R3030 T10866 T10865 nummod 16,%
R3031 T10867 T10865 punct ),%
R3032 T10868 T10863 prep of,13
R3033 T10869 T10868 pobj these,of
R3034 T10870 T10871 det a,increase
R3035 T10871 T10845 dobj increase,demonstrated
R3036 T10872 T10871 amod marked,increase
R3037 T10873 T10871 prep in,increase
R3038 T10874 T10875 amod erythroid,cells
R3039 T10875 T10873 pobj cells,in
R3040 T10876 T10877 mark with,in
R3041 T10877 T10845 advcl in,demonstrated
R3042 T10878 T10879 quantmod 100,"1,000"
R3043 T10879 T10881 nummod "1,000",cells
R3044 T10880 T10879 punct –,"1,000"
R3045 T10881 T10877 nsubj cells,in
R3046 T10882 T10881 compound GFP+,cells
R3047 T10883 T10877 pobj circulation,in
R3048 T10884 T10885 nummod 6,d
R3049 T10885 T10886 npadvmod d,later
R3050 T10886 T10845 advmod later,demonstrated
R3051 T10887 T10845 punct .,demonstrated
R3052 T10889 T10890 prep By,showed
R3053 T10891 T10889 pobj day,By
R3054 T10892 T10891 nummod 10,day
R3055 T10893 T10890 punct ", ",showed
R3056 T10894 T10895 det these,embryos
R3057 T10895 T10890 nsubj embryos,showed
R3058 T10896 T10895 amod transplanted,embryos
R3059 T10897 T10898 advmod approximately,"3,000"
R3060 T10898 T10899 nummod "3,000",cells
R3061 T10899 T10890 dobj cells,showed
R3062 T10900 T10899 prep in,cells
R3063 T10901 T10900 pobj circulation,in
R3064 T10902 T10890 punct ", ",showed
R3065 T10903 T10890 advcl similar,showed
R3066 T10904 T10903 prep to,similar
R3067 T10905 T10906 det the,number
R3068 T10906 T10904 pobj number,to
R3069 T10907 T10906 prep of,number
R3070 T10908 T10909 compound blood,cells
R3071 T10909 T10907 pobj cells,of
R3072 T10910 T10906 prep in,number
R3073 T10911 T10912 amod normal,embryos
R3074 T10912 T10910 pobj embryos,in
R3075 T10913 T10890 punct .,showed
R3076 T10915 T10916 prep Despite,inflate
R3077 T10917 T10918 amod robust,reconstitution
R3078 T10918 T10915 pobj reconstitution,Despite
R3079 T10919 T10918 prep of,reconstitution
R3080 T10920 T10921 compound blood,cells
R3081 T10921 T10919 pobj cells,of
R3082 T10922 T10916 punct ", ",inflate
R3083 T10923 T10924 amod mutant,recipients
R3084 T10924 T10916 nsubj recipients,inflate
R3085 T10925 T10916 aux did,inflate
R3086 T10926 T10916 neg not,inflate
R3087 T10927 T10928 poss their,bladders
R3088 T10928 T10916 dobj bladders,inflate
R3089 T10929 T10928 compound swim,bladders
R3090 T10930 T10916 cc and,inflate
R3091 T10931 T10932 advmod thus,failed
R3092 T10932 T10916 conj failed,inflate
R3093 T10933 T10934 aux to,survive
R3094 T10934 T10932 xcomp survive,failed
R3095 T10935 T10934 advmod longer,survive
R3096 T10936 T10935 prep than,longer
R3097 T10937 T10938 amod nontransplanted,controls
R3098 T10938 T10936 pobj controls,than
R3099 T10939 T10938 compound sibling,controls
R3100 T10940 T10934 punct ", ",survive
R3101 T10941 T10942 nsubj all,dying
R3102 T10942 T10934 advcl dying,survive
R3103 T10943 T10942 prep by,dying
R3104 T10944 T10945 nummod 3,wk
R3105 T10945 T10943 pobj wk,by
R3106 T10946 T10945 prep of,wk
R3107 T10947 T10946 pobj age,of
R3108 T10948 T10916 punct .,inflate
R3109 T10950 T10951 prep In,survived
R3110 T10952 T10950 pobj contrast,In
R3111 T10953 T10951 punct ", ",survived
R3112 T10954 T10955 quantmod 13,35
R3113 T10955 T10957 nummod 35,transplants
R3114 T10956 T10955 punct /,35
R3115 T10957 T10951 nsubj transplants,survived
R3116 T10958 T10959 punct (,%
R3117 T10959 T10957 parataxis %,transplants
R3118 T10960 T10959 nummod 37,%
R3119 T10961 T10959 punct ),%
R3120 T10962 T10957 amod heterozygous,transplants
R3121 T10963 T10957 compound montg234,transplants
R3122 T10964 T10951 prep to,survived
R3123 T10965 T10966 amod early,adulthood
R3124 T10966 T10964 pobj adulthood,to
R3125 T10967 T10951 punct .,survived
R3126 T10969 T10970 amod Similar,transplants
R3127 T10970 T10971 nsubj transplants,rescue
R3128 T10972 T10970 prep of,transplants
R3129 T10973 T10974 amod wild,type
R3130 T10974 T10976 compound type,cells
R3131 T10975 T10974 punct -,type
R3132 T10976 T10972 pobj cells,of
R3133 T10977 T10971 aux can,rescue
R3134 T10978 T10971 advmod fully,rescue
R3135 T10979 T10980 nmod vlad,tepes
R3136 T10980 T10981 nmod tepes,mutants
R3137 T10981 T10971 dobj mutants,rescue
R3138 T10982 T10980 punct (,tepes
R3139 T10983 T10980 appos gata1,tepes
R3140 T10984 T10981 punct ),mutants
R3141 T10985 T10986 punct (,Traver
R3142 T10986 T10971 meta Traver,rescue
R3143 T10987 T10986 nmod et,Traver
R3144 T10988 T10986 nmod al.,Traver
R3145 T10989 T10986 nummod 2003,Traver
R3146 T10990 T10986 punct ),Traver
R3147 T10991 T10971 punct .,rescue
R3148 T10993 T10994 advmod Therefore,suggests
R3149 T10995 T10994 punct ", ",suggests
R3150 T10996 T10997 det the,results
R3151 T10997 T10994 nsubj results,suggests
R3152 T10998 T10997 prep of,results
R3153 T10999 T11000 compound cell,transplantations
R3154 T11000 T10998 pobj transplantations,of
R3155 T11001 T11002 mark that,plays
R3156 T11002 T10994 ccomp plays,suggests
R3157 T11003 T11002 nsubj tif1γ,plays
R3158 T11004 T11005 det a,role
R3159 T11005 T11002 dobj role,plays
R3160 T11006 T11007 npadvmod cell,autonomous
R3161 T11007 T11005 amod autonomous,role
R3162 T11008 T11007 punct -,autonomous
R3163 T11009 T11002 prep in,plays
R3164 T11010 T11011 amod erythroid,cells
R3165 T11011 T11009 pobj cells,in
R3166 T11012 T10994 punct ", ",suggests
R3167 T11013 T10994 cc and,suggests
R3168 T11014 T11015 poss its,role
R3169 T11015 T11016 nsubjpass role,required
R3170 T11016 T10994 conj required,suggests
R3171 T11017 T11015 prep in,role
R3172 T11018 T11019 amod nonhematopoietic,tissues
R3173 T11019 T11017 pobj tissues,in
R3174 T11020 T11019 punct ", ",tissues
R3175 T11021 T11022 amod such,as
R3176 T11022 T11019 prep as,tissues
R3177 T11023 T11024 compound trunk,mesenchyme
R3178 T11024 T11022 pobj mesenchyme,as
R3179 T11025 T11024 cc or,mesenchyme
R3180 T11026 T11027 det the,system
R3181 T11027 T11024 conj system,mesenchyme
R3182 T11028 T11027 amod nervous,system
R3183 T11029 T11016 punct ", ",required
R3184 T11030 T11016 auxpass is,required
R3185 T11031 T11016 advmod also,required
R3186 T11032 T11016 prep for,required
R3187 T11033 T11034 compound embryo,survival
R3188 T11034 T11032 pobj survival,for
R3189 T11035 T11016 punct .,required
R3198 T11963 T11964 nsubjpass Tif1γ,Regulated
R3199 T11965 T11963 prep in,Tif1γ
R3200 T11966 T11967 nmod Punctate,Foci
R3201 T11967 T11965 pobj Foci,in
R3202 T11968 T11967 amod Nuclear,Foci
R3203 T11969 T11964 auxpass Is,Regulated
R3204 T11970 T11964 advmod Developmentally,Regulated
R3205 T11972 T11973 prep In,generated
R3206 T11974 T11972 pobj order,In
R3207 T11975 T11976 aux to,examine
R3208 T11976 T11974 acl examine,order
R3209 T11977 T11978 det the,distribution
R3210 T11978 T11976 dobj distribution,examine
R3211 T11979 T11978 amod subcellular,distribution
R3212 T11980 T11978 prep of,distribution
R3213 T11981 T11982 compound Tif1γ,protein
R3214 T11982 T11980 pobj protein,of
R3215 T11983 T11973 punct ", ",generated
R3216 T11984 T11973 nsubj we,generated
R3217 T11985 T11986 det an,antiserum
R3218 T11986 T11973 dobj antiserum,generated
R3219 T11987 T11988 npadvmod affinity,purified
R3220 T11988 T11986 amod purified,antiserum
R3221 T11989 T11988 punct -,purified
R3222 T11990 T11986 nmod rabbit,antiserum
R3223 T11991 T11986 amod polyclonal,antiserum
R3224 T11992 T11986 acl directed,antiserum
R3225 T11993 T11992 prep against,directed
R3226 T11994 T11995 det the,acids
R3227 T11995 T11993 pobj acids,against
R3228 T11996 T11997 npadvmod C,terminal
R3229 T11997 T11995 amod terminal,acids
R3230 T11998 T11997 punct -,terminal
R3231 T11999 T11995 nummod 15,acids
R3232 T12000 T11995 compound amino,acids
R3233 T12001 T11995 acl conserved,acids
R3234 T12002 T12001 prep in,conserved
R3235 T12003 T12004 amod human,TIF1γ
R3236 T12004 T12002 pobj TIF1γ,in
R3237 T12005 T12004 cc and,TIF1γ
R3238 T12006 T12007 compound mouse,Tif1γ
R3239 T12007 T12004 conj Tif1γ,TIF1γ
R3240 T12008 T11973 punct .,generated
R3241 T12010 T12011 nsubj Immunofluorescence,demonstrates
R3242 T12012 T12010 prep of,Immunofluorescence
R3243 T12013 T12014 compound mouse,embryo
R3244 T12014 T12015 compound embryo,fibroblast
R3245 T12015 T12016 compound fibroblast,nuclei
R3246 T12016 T12012 pobj nuclei,of
R3247 T12017 T12010 prep with,Immunofluorescence
R3248 T12018 T12019 det the,antiserum
R3249 T12019 T12017 pobj antiserum,with
R3250 T12020 T12019 amod anti-Tif1γ,antiserum
R3251 T12021 T12022 mark that,localized
R3252 T12022 T12011 ccomp localized,demonstrates
R3253 T12023 T12022 nsubjpass Tif1γ,localized
R3254 T12024 T12022 auxpass is,localized
R3255 T12025 T12022 prep in,localized
R3256 T12026 T12027 amod small,foci
R3257 T12027 T12025 pobj foci,in
R3258 T12028 T12027 amod nuclear,foci
R3259 T12029 T12030 punct (,Figure
R3260 T12030 T12011 parataxis Figure,demonstrates
R3261 T12031 T12030 nummod 6A,Figure
R3262 T12032 T12030 punct ),Figure
R3263 T12033 T12011 punct .,demonstrates
R3264 T12035 T12036 det The,localization
R3265 T12036 T12037 nsubj localization,appears
R3266 T12038 T12036 prep of,localization
R3267 T12039 T12040 compound Tif1γ,protein
R3268 T12040 T12038 pobj protein,of
R3269 T12041 T12037 oprd different,appears
R3270 T12042 T12041 prep from,different
R3271 T12043 T12044 det the,patterns
R3272 T12044 T12042 pobj patterns,from
R3273 T12045 T12046 advmod more,diffuse
R3274 T12046 T12044 amod diffuse,patterns
R3275 T12047 T12044 amod nuclear,patterns
R3276 T12048 T12049 advmod typically,seen
R3277 T12049 T12044 acl seen,patterns
R3278 T12050 T12049 prep in,seen
R3279 T12051 T12050 pobj studies,in
R3280 T12052 T12051 prep of,studies
R3281 T12053 T12052 pobj Tif1α,of
R3282 T12054 T12055 punct (,Remboutsika
R3283 T12055 T12053 meta Remboutsika,Tif1α
R3284 T12056 T12055 nmod et,Remboutsika
R3285 T12057 T12055 nmod al.,Remboutsika
R3286 T12058 T12055 nummod 2002,Remboutsika
R3287 T12059 T12055 punct ),Remboutsika
R3288 T12060 T12053 cc or,Tif1α
R3289 T12061 T12053 conj TIF1β,Tif1α
R3290 T12062 T12063 punct (,Cammas
R3291 T12063 T12061 meta Cammas,TIF1β
R3292 T12064 T12063 nmod et,Cammas
R3293 T12065 T12063 nmod al.,Cammas
R3294 T12066 T12063 nummod 2002,Cammas
R3295 T12067 T12063 punct ),Cammas
R3296 T12068 T12037 punct .,appears
R3297 T12070 T12071 det A,report
R3298 T12071 T12073 nsubj report,demonstrates
R3299 T12072 T12071 amod recent,report
R3300 T12074 T12075 mark that,associates
R3301 T12075 T12073 ccomp associates,demonstrates
R3302 T12076 T12075 nsubj TIF1β,associates
R3303 T12077 T12075 prep with,associates
R3304 T12078 T12079 npadvmod heterochromatin,containing
R3305 T12079 T12081 amod containing,foci
R3306 T12080 T12079 punct -,containing
R3307 T12081 T12077 pobj foci,with
R3308 T12082 T12075 prep after,associates
R3309 T12083 T12084 amod retinoic,acid
R3310 T12084 T12085 compound acid,treatment
R3311 T12085 T12082 pobj treatment,after
R3312 T12086 T12085 cc or,treatment
R3313 T12087 T12088 compound serum,starvation
R3314 T12088 T12085 conj starvation,treatment
R3315 T12089 T12090 punct (,Cammas
R3316 T12090 T12073 meta Cammas,demonstrates
R3317 T12091 T12090 nmod et,Cammas
R3318 T12092 T12090 nmod al.,Cammas
R3319 T12093 T12090 nummod 2002,Cammas
R3320 T12094 T12090 punct ),Cammas
R3321 T12095 T12073 punct .,demonstrates
R3322 T12097 T12098 advmod Thus,regulated
R3323 T12099 T12098 punct ", ",regulated
R3324 T12100 T12098 nsubjpass localization,regulated
R3325 T12101 T12100 cc or,localization
R3326 T12102 T12100 conj expression,localization
R3327 T12103 T12100 prep of,localization
R3328 T12104 T12105 det the,proteins
R3329 T12105 T12103 pobj proteins,of
R3330 T12106 T12105 compound TIF1,proteins
R3331 T12107 T12098 aux may,regulated
R3332 T12108 T12098 auxpass be,regulated
R3333 T12109 T12098 prep during,regulated
R3334 T12110 T12111 amod distinct,processes
R3335 T12111 T12109 pobj processes,during
R3336 T12112 T12111 amod developmental,processes
R3337 T12113 T12109 cc or,during
R3338 T12114 T12109 conj by,during
R3339 T12115 T12116 amod environmental,cues
R3340 T12116 T12114 pobj cues,by
R3341 T12117 T12098 punct .,regulated
R3342 T12119 T12120 det The,foci
R3343 T12120 T12122 nsubj foci,colocalize
R3344 T12121 T12120 amod nuclear,foci
R3345 T12123 T12124 dep that,contain
R3346 T12124 T12120 relcl contain,foci
R3347 T12125 T12124 dobj Tif1γ,contain
R3348 T12126 T12122 aux do,colocalize
R3349 T12127 T12122 neg not,colocalize
R3350 T12128 T12122 prep with,colocalize
R3351 T12129 T12130 nummod two,markers
R3352 T12130 T12128 pobj markers,with
R3353 T12131 T12130 prep of,markers
R3354 T12132 T12131 pobj heterochromatin,of
R3355 T12133 T12130 punct ", ",markers
R3356 T12134 T12135 compound HP1α,protein
R3357 T12135 T12130 appos protein,markers
R3358 T12136 T12135 cc and,protein
R3359 T12137 T12138 compound DAPI,staining
R3360 T12138 T12135 conj staining,protein
R3361 T12139 T12138 prep of,staining
R3362 T12140 T12139 pobj DNA,of
R3363 T12141 T12142 punct (,Figure
R3364 T12142 T12122 parataxis Figure,colocalize
R3365 T12143 T12142 nummod 6A,Figure
R3366 T12144 T12142 punct ),Figure
R3367 T12145 T12122 punct .,colocalize
R3368 T12147 T12148 advmod Furthermore,colocalize
R3369 T12149 T12148 punct ", ",colocalize
R3370 T12150 T12148 nsubj Tif1γ,colocalize
R3371 T12151 T12148 aux does,colocalize
R3372 T12152 T12148 neg not,colocalize
R3373 T12153 T12148 prep with,colocalize
R3374 T12154 T12155 amod promyelocytic,product
R3375 T12155 T12158 nmod product,bodies
R3376 T12156 T12155 nmod leukemia,product
R3377 T12157 T12155 nmod gene,product
R3378 T12158 T12153 pobj bodies,with
R3379 T12159 T12155 punct (,product
R3380 T12160 T12155 appos PML,product
R3381 T12161 T12158 punct ),bodies
R3382 T12162 T12158 amod nuclear,bodies
R3383 T12163 T12158 punct ", ",bodies
R3384 T12164 T12165 compound DNA,complexes
R3385 T12165 T12158 conj complexes,bodies
R3386 T12166 T12165 compound repair,complexes
R3387 T12167 T12168 dep that,contain
R3388 T12168 T12165 relcl contain,complexes
R3389 T12169 T12168 dobj Mre11,contain
R3390 T12170 T12165 punct ", ",complexes
R3391 T12171 T12165 cc or,complexes
R3392 T12172 T12173 amod transcriptional,complexes
R3393 T12173 T12165 conj complexes,complexes
R3394 T12174 T12173 acl containing,complexes
R3395 T12175 T12176 compound TFII,B
R3396 T12176 T12174 dobj B,containing
R3397 T12177 T12176 punct -,B
R3398 T12178 T12179 punct (,data
R3399 T12179 T12148 parataxis data,colocalize
R3400 T12180 T12179 amod unpublished,data
R3401 T12181 T12179 punct ),data
R3402 T12182 T12148 punct .,colocalize
R3403 T12184 T12185 nsubj We,examined
R3404 T12186 T12185 advmod next,examined
R3405 T12187 T12188 det the,expression
R3406 T12188 T12185 dobj expression,examined
R3407 T12189 T12188 prep of,expression
R3408 T12190 T12191 compound Tif1γ,protein
R3409 T12191 T12189 pobj protein,of
R3410 T12192 T12185 prep during,examined
R3411 T12193 T12194 det the,differentiation
R3412 T12194 T12192 pobj differentiation,during
R3413 T12195 T12194 prep of,differentiation
R3414 T12196 T12197 compound G1E,cells
R3415 T12197 T12195 pobj cells,of
R3416 T12198 T12197 punct ", ",cells
R3417 T12199 T12200 det a,line
R3418 T12200 T12197 appos line,cells
R3419 T12201 T12200 amod murine,line
R3420 T12202 T12200 compound erythroleukemia,line
R3421 T12203 T12200 compound cell,line
R3422 T12204 T12205 dep that,differentiate
R3423 T12205 T12200 relcl differentiate,line
R3424 T12206 T12205 aux can,differentiate
R3425 T12207 T12205 advmod terminally,differentiate
R3426 T12208 T12205 prep into,differentiate
R3427 T12209 T12208 pobj erythrocytes,into
R3428 T12210 T12211 advmod when,stabilized
R3429 T12211 T12205 advcl stabilized,differentiate
R3430 T12212 T12213 det a,protein
R3431 T12213 T12211 nsubjpass protein,stabilized
R3432 T12214 T12215 nmod Gata1,estrogen
R3433 T12215 T12217 compound estrogen,receptor
R3434 T12216 T12215 punct :,estrogen
R3435 T12217 T12213 compound receptor,protein
R3436 T12218 T12213 compound fusion,protein
R3437 T12219 T12211 auxpass is,stabilized
R3438 T12220 T12211 prep in,stabilized
R3439 T12221 T12220 pobj response,in
R3440 T12222 T12221 prep to,response
R3441 T12223 T12224 compound estrogen,exposure
R3442 T12224 T12222 pobj exposure,to
R3443 T12225 T12226 punct (,Weiss
R3444 T12226 T12185 meta Weiss,examined
R3445 T12227 T12226 nmod et,Weiss
R3446 T12228 T12226 nmod al.,Weiss
R3447 T12229 T12226 nummod 1997,Weiss
R3448 T12230 T12226 punct ),Weiss
R3449 T12231 T12185 punct .,examined
R3450 T12233 T12234 compound Western,blot
R3451 T12234 T12235 compound blot,analysis
R3452 T12235 T12236 nsubj analysis,demonstrated
R3453 T12237 T12238 mark that,decreases
R3454 T12238 T12236 ccomp decreases,demonstrated
R3455 T12239 T12240 compound Tif1γ,expression
R3456 T12240 T12238 nsubj expression,decreases
R3457 T12241 T12240 compound protein,expression
R3458 T12242 T12238 prep with,decreases
R3459 T12243 T12244 amod terminal,erythroid
R3460 T12244 T12245 compound erythroid,differentiation
R3461 T12245 T12242 pobj differentiation,with
R3462 T12246 T12247 punct (,Figure
R3463 T12247 T12236 parataxis Figure,demonstrated
R3464 T12248 T12247 nummod 6B,Figure
R3465 T12249 T12247 punct ),Figure
R3466 T12250 T12236 punct .,demonstrated
R3467 T12252 T12253 advcl Consistent,falls
R3468 T12254 T12252 prep with,Consistent
R3469 T12255 T12256 det this,finding
R3470 T12256 T12254 pobj finding,with
R3471 T12257 T12253 punct ", ",falls
R3472 T12258 T12253 prep after,falls
R3473 T12259 T12260 nummod 24,hpf
R3474 T12260 T12258 pobj hpf,after
R3475 T12261 T12253 punct ", ",falls
R3476 T12262 T12263 compound zebrafish,mRNA
R3477 T12263 T12265 compound mRNA,expression
R3478 T12264 T12263 compound mon,mRNA
R3479 T12265 T12253 nsubj expression,falls
R3480 T12266 T12253 prep during,falls
R3481 T12267 T12268 det the,maturation
R3482 T12268 T12266 pobj maturation,during
R3483 T12269 T12268 amod terminal,maturation
R3484 T12270 T12268 prep of,maturation
R3485 T12271 T12272 det the,cells
R3486 T12272 T12270 pobj cells,of
R3487 T12273 T12272 amod primitive,cells
R3488 T12274 T12272 amod erythroid,cells
R3489 T12275 T12276 punct (,data
R3490 T12276 T12253 parataxis data,falls
R3491 T12277 T12276 amod unpublished,data
R3492 T12278 T12276 punct ),data
R3493 T12279 T12253 punct .,falls
R3494 T12281 T12282 prep In,expressed
R3495 T12283 T12284 nummod two,lines
R3496 T12284 T12281 pobj lines,In
R3497 T12285 T12284 amod different,lines
R3498 T12286 T12284 amod murine,lines
R3499 T12287 T12284 compound erythroleukemia,lines
R3500 T12288 T12284 compound cell,lines
R3501 T12289 T12284 punct (,lines
R3502 T12290 T12284 appos MEL,lines
R3503 T12291 T12290 cc and,MEL
R3504 T12292 T12290 conj G1E,MEL
R3505 T12293 T12282 punct ),expressed
R3506 T12294 T12282 punct ", ",expressed
R3507 T12295 T12282 nsubjpass Tif1γ,expressed
R3508 T12296 T12282 auxpass is,expressed
R3509 T12297 T12282 advmod also,expressed
R3510 T12298 T12282 prep in,expressed
R3511 T12299 T12300 amod nuclear,foci
R3512 T12300 T12298 pobj foci,in
R3513 T12301 T12282 punct ", ",expressed
R3514 T12302 T12282 cc and,expressed
R3515 T12303 T12304 advmod even,reduced
R3516 T12304 T12312 advcl reduced,change
R3517 T12305 T12304 mark though,reduced
R3518 T12306 T12307 det the,level
R3519 T12307 T12304 nsubjpass level,reduced
R3520 T12308 T12307 amod overall,level
R3521 T12309 T12307 compound Tif1γ,level
R3522 T12310 T12307 compound protein,level
R3523 T12311 T12304 auxpass is,reduced
R3524 T12312 T12282 conj change,expressed
R3525 T12313 T12312 punct ", ",change
R3526 T12314 T12315 det this,localization
R3527 T12315 T12312 nsubj localization,change
R3528 T12316 T12315 amod nuclear,localization
R3529 T12317 T12315 compound foci,localization
R3530 T12318 T12312 aux does,change
R3531 T12319 T12312 neg not,change
R3532 T12320 T12312 prep with,change
R3533 T12321 T12320 pobj differentiation,with
R3534 T12322 T12323 punct (,data
R3535 T12323 T12312 parataxis data,change
R3536 T12324 T12323 amod unpublished,data
R3537 T12325 T12323 punct ),data
R3538 T12326 T12312 punct .,change
R3539 T12328 T12329 nsubj This,provides
R3540 T12330 T12331 amod further,support
R3541 T12331 T12329 dobj support,provides
R3542 T12332 T12329 prep for,provides
R3543 T12333 T12334 det the,hypothesis
R3544 T12334 T12332 pobj hypothesis,for
R3545 T12335 T12336 mark that,acts
R3546 T12336 T12334 acl acts,hypothesis
R3547 T12337 T12336 nsubj Tif1γ,acts
R3548 T12338 T12336 prep within,acts
R3549 T12339 T12340 amod novel,foci
R3550 T12340 T12338 pobj foci,within
R3551 T12341 T12340 amod nuclear,foci
R3552 T12342 T12336 punct ", ",acts
R3553 T12343 T12336 prep during,acts
R3554 T12344 T12345 amod erythroid,differentiation
R3555 T12345 T12343 pobj differentiation,during
R3556 T12346 T12329 punct .,provides
R3557 T12542 T12543 det The,Zebrafish
R3558 T12543 T12544 nsubj Zebrafish,is
R3559 T12545 T12546 det an,system
R3560 T12546 T12544 attr system,is
R3561 T12547 T12546 amod excellent,system
R3562 T12548 T12546 compound model,system
R3563 T12549 T12550 aux to,elucidate
R3564 T12550 T12546 advcl elucidate,system
R3565 T12551 T12552 det the,machinery
R3566 T12552 T12550 dobj machinery,elucidate
R3567 T12553 T12552 amod molecular,machinery
R3568 T12554 T12552 acl controlling,machinery
R3569 T12555 T12556 compound gene,expression
R3570 T12556 T12554 dobj expression,controlling
R3571 T12557 T12554 prep during,controlling
R3572 T12558 T12557 pobj hematopoiesis,during
R3573 T12559 T12560 punct (,Thisse
R3574 T12560 T12544 meta Thisse,is
R3575 T12561 T12560 cc and,Thisse
R3576 T12562 T12560 conj Zon,Thisse
R3577 T12563 T12562 nummod 2002,Zon
R3578 T12564 T12562 punct ;,Zon
R3579 T12565 T12562 conj Galloway,Zon
R3580 T12566 T12565 cc and,Galloway
R3581 T12567 T12565 conj Zon,Galloway
R3582 T12568 T12567 nummod 2003,Zon
R3583 T12569 T12567 punct ),Zon
R3584 T12570 T12544 punct .,is
R3585 T12572 T12573 prep As,identified
R3586 T12574 T12572 pobj part,As
R3587 T12575 T12574 prep of,part
R3588 T12576 T12577 det a,screen
R3589 T12577 T12575 pobj screen,of
R3590 T12578 T12579 amod large,scale
R3591 T12579 T12577 nmod scale,screen
R3592 T12580 T12579 punct -,scale
R3593 T12581 T12577 amod forward,screen
R3594 T12582 T12577 amod genetic,screen
R3595 T12583 T12573 punct ", ",identified
R3596 T12584 T12573 nsubj we,identified
R3597 T12585 T12573 advmod originally,identified
R3598 T12586 T12587 det a,group
R3599 T12587 T12573 dobj group,identified
R3600 T12588 T12587 compound complementation,group
R3601 T12589 T12587 prep of,group
R3602 T12590 T12591 amod independent,alleles
R3603 T12591 T12589 pobj alleles,of
R3604 T12592 T12591 compound mutant,alleles
R3605 T12593 T12587 prep in,group
R3606 T12594 T12595 det the,gene
R3607 T12595 T12593 pobj gene,in
R3608 T12596 T12595 compound zebrafish,gene
R3609 T12597 T12598 dep that,named
R3610 T12598 T12587 relcl named,group
R3611 T12599 T12598 nsubj we,named
R3612 T12600 T12598 oprd moonshine,named
R3613 T12601 T12602 punct (,Ransom
R3614 T12602 T12573 meta Ransom,identified
R3615 T12603 T12602 nmod et,Ransom
R3616 T12604 T12602 nmod al.,Ransom
R3617 T12605 T12602 nummod 1996,Ransom
R3618 T12606 T12602 punct ),Ransom
R3619 T12607 T12573 punct .,identified
R3620 T12609 T12610 amod Positional,cloning
R3621 T12610 T12611 nsubjpass cloning,used
R3622 T12612 T12611 auxpass was,used
R3623 T12613 T12614 aux to,identify
R3624 T12614 T12611 advcl identify,used
R3625 T12615 T12616 det the,gene
R3626 T12616 T12614 dobj gene,identify
R3627 T12617 T12616 compound mon,gene
R3628 T12618 T12611 punct ", ",used
R3629 T12619 T12611 advcl establishing,used
R3630 T12620 T12621 det a,role
R3631 T12621 T12619 dobj role,establishing
R3632 T12622 T12621 amod critical,role
R3633 T12623 T12619 prep for,establishing
R3634 T12624 T12625 det a,factor
R3635 T12625 T12623 pobj factor,for
R3636 T12626 T12625 amod transcriptional,factor
R3637 T12627 T12625 amod intermediary,factor
R3638 T12628 T12625 punct ", ",factor
R3639 T12629 T12625 appos Tif1γ,factor
R3640 T12630 T12619 punct ", ",establishing
R3641 T12631 T12619 prep during,establishing
R3642 T12632 T12633 amod hematopoietic,development
R3643 T12633 T12631 pobj development,during
R3644 T12634 T12611 punct .,used
R3651 T13586 T13587 det The,Gene
R3652 T13587 T13589 nsubj Gene,Encodes
R3653 T13588 T13587 compound mon,Gene
R3654 T13590 T13591 det the,Ortholog
R3655 T13591 T13589 dobj Ortholog,Encodes
R3656 T13592 T13591 compound Zebrafish,Ortholog
R3657 T13593 T13591 prep of,Ortholog
R3658 T13594 T13595 amod Mammalian,TIF1γ
R3659 T13595 T13593 pobj TIF1γ,of
R3660 T13597 T13598 poss Our,results
R3661 T13598 T13599 nsubj results,support
R3662 T13600 T13599 advmod strongly,support
R3663 T13601 T13602 det the,conclusion
R3664 T13602 T13599 dobj conclusion,support
R3665 T13603 T13604 mark that,cloned
R3666 T13604 T13602 acl cloned,conclusion
R3667 T13605 T13604 nsubj we,cloned
R3668 T13606 T13604 aux have,cloned
R3669 T13607 T13604 advmod positionally,cloned
R3670 T13608 T13609 det the,gene
R3671 T13609 T13604 dobj gene,cloned
R3672 T13610 T13609 compound zebrafish,gene
R3673 T13611 T13609 compound mon,gene
R3674 T13612 T13604 advmod correctly,cloned
R3675 T13613 T13599 punct ", ",support
R3676 T13614 T13599 cc and,support
R3677 T13615 T13616 nsubj it,is
R3678 T13616 T13599 conj is,support
R3679 T13617 T13618 det the,ortholog
R3680 T13618 T13616 attr ortholog,is
R3681 T13619 T13618 prep of,ortholog
R3682 T13620 T13621 amod mammalian,Tif1γ
R3683 T13621 T13619 pobj Tif1γ,of
R3684 T13622 T13616 punct .,is
R3685 T13624 T13625 nsubj Tif1γ,is
R3686 T13626 T13625 acomp present,is
R3687 T13627 T13625 prep in,is
R3688 T13628 T13629 det the,interval
R3689 T13629 T13627 pobj interval,in
R3690 T13630 T13629 amod critical,interval
R3691 T13631 T13629 amod genetic,interval
R3692 T13632 T13629 acl encompassing,interval
R3693 T13633 T13634 det a,clone
R3694 T13634 T13632 dobj clone,encompassing
R3695 T13635 T13634 amod single,clone
R3696 T13636 T13637 advmod approximately,50
R3697 T13637 T13638 nummod 50,kb
R3698 T13638 T13634 compound kb,clone
R3699 T13639 T13638 punct -,kb
R3700 T13640 T13634 compound PAC,clone
R3701 T13641 T13634 acl defined,clone
R3702 T13642 T13641 prep by,defined
R3703 T13643 T13644 compound linkage,analysis
R3704 T13644 T13642 pobj analysis,by
R3705 T13645 T13646 punct (,see
R3706 T13646 T13625 parataxis see,is
R3707 T13647 T13646 dobj Figure,see
R3708 T13648 T13647 nummod 3,Figure
R3709 T13649 T13646 punct ),see
R3710 T13650 T13625 punct .,is
R3711 T13652 T13653 compound Sequence,analysis
R3712 T13653 T13654 nsubj analysis,indicates
R3713 T13655 T13656 mark that,is
R3714 T13656 T13654 ccomp is,indicates
R3715 T13657 T13658 compound zebrafish,tif1γ
R3716 T13658 T13656 nsubj tif1γ,is
R3717 T13659 T13660 advmod most,similar
R3718 T13660 T13656 acomp similar,is
R3719 T13661 T13660 prep in,similar
R3720 T13662 T13663 amod predicted,sequence
R3721 T13663 T13661 pobj sequence,in
R3722 T13664 T13663 compound amino,sequence
R3723 T13665 T13663 compound acid,sequence
R3724 T13666 T13663 cc and,sequence
R3725 T13667 T13668 compound intron,exon
R3726 T13668 T13670 compound exon,structure
R3727 T13669 T13668 punct /,exon
R3728 T13670 T13663 conj structure,sequence
R3729 T13671 T13656 prep compared,is
R3730 T13672 T13671 prep to,compared
R3731 T13673 T13674 det the,genes
R3732 T13674 T13672 pobj genes,to
R3733 T13675 T13674 amod predicted,genes
R3734 T13676 T13674 amod orthologous,genes
R3735 T13677 T13674 amod human,genes
R3736 T13678 T13677 cc and,human
R3737 T13679 T13677 conj mouse,human
R3738 T13680 T13654 punct .,indicates
R3739 T13682 T13683 compound Zebrafish,tif1γ
R3740 T13683 T13684 nsubjpass tif1γ,located
R3741 T13685 T13684 auxpass is,located
R3742 T13686 T13684 prep in,located
R3743 T13687 T13688 det a,region
R3744 T13688 T13686 pobj region,in
R3745 T13689 T13688 prep of,region
R3746 T13690 T13691 compound zebrafish,Chromosome
R3747 T13691 T13689 pobj Chromosome,of
R3748 T13692 T13691 nummod 8,Chromosome
R3749 T13693 T13691 amod syntenic,Chromosome
R3750 T13694 T13693 prep to,syntenic
R3751 T13695 T13696 det the,region
R3752 T13696 T13694 pobj region,to
R3753 T13697 T13696 prep of,region
R3754 T13698 T13699 amod human,Chromosome
R3755 T13699 T13697 pobj Chromosome,of
R3756 T13700 T13699 nummod 1,Chromosome
R3757 T13701 T13699 acl containing,Chromosome
R3758 T13702 T13701 dobj TIF1γ,containing
R3759 T13703 T13684 punct .,located
R3760 T13705 T13706 nsubj We,identified
R3761 T13707 T13708 compound point,mutations
R3762 T13708 T13706 dobj mutations,identified
R3763 T13709 T13708 prep in,mutations
R3764 T13710 T13709 pobj tif1γ,in
R3765 T13711 T13708 prep from,mutations
R3766 T13712 T13713 nummod three,alleles
R3767 T13713 T13711 pobj alleles,from
R3768 T13714 T13713 amod different,alleles
R3769 T13715 T13713 prep of,alleles
R3770 T13716 T13715 pobj mon,of
R3771 T13717 T13718 dep that,result
R3772 T13718 T13713 relcl result,alleles
R3773 T13719 T13717 nmod each,that
R3774 T13720 T13718 prep in,result
R3775 T13721 T13722 amod premature,codons
R3776 T13722 T13720 pobj codons,in
R3777 T13723 T13722 compound stop,codons
R3778 T13724 T13722 cc and,codons
R3779 T13725 T13726 compound mRNA,decay
R3780 T13726 T13722 conj decay,codons
R3781 T13727 T13706 punct .,identified
R3782 T13729 T13730 prep In,is
R3783 T13731 T13729 pobj addition,In
R3784 T13732 T13730 punct ", ",is
R3785 T13733 T13734 compound tif1γ,Tif1γ
R3786 T13734 T13730 nsubj Tif1γ,is
R3787 T13735 T13734 punct /,Tif1γ
R3788 T13736 T13737 advmod highly,expressed
R3789 T13737 T13730 acomp expressed,is
R3790 T13738 T13730 prep in,is
R3791 T13739 T13740 amod hematopoietic,cells
R3792 T13740 T13738 pobj cells,in
R3793 T13741 T13730 prep throughout,is
R3794 T13742 T13741 pobj embryogenesis,throughout
R3795 T13743 T13730 prep in,is
R3796 T13744 T13745 preconj both,zebrafish
R3797 T13745 T13743 pobj zebrafish,in
R3798 T13746 T13745 cc and,zebrafish
R3799 T13747 T13745 conj mouse,zebrafish
R3800 T13748 T13749 punct (,see
R3801 T13749 T13730 parataxis see,is
R3802 T13750 T13749 dobj Figure,see
R3803 T13751 T13750 nummod 4,Figure
R3804 T13752 T13749 punct ),see
R3805 T13753 T13730 punct .,is
R3806 T13755 T13756 cc And,rescues
R3807 T13757 T13758 mark as,predicted
R3808 T13758 T13756 advcl predicted,rescues
R3809 T13759 T13756 punct ", ",rescues
R3810 T13760 T13761 amod forced,expression
R3811 T13761 T13756 nsubj expression,rescues
R3812 T13762 T13761 prep of,expression
R3813 T13763 T13764 amod wild,type
R3814 T13764 T13766 compound type,mRNA
R3815 T13765 T13764 punct -,type
R3816 T13766 T13762 pobj mRNA,of
R3817 T13767 T13766 compound tif1γ,mRNA
R3818 T13768 T13756 advmod efficiently,rescues
R3819 T13769 T13756 dobj hematopoiesis,rescues
R3820 T13770 T13756 prep in,rescues
R3821 T13771 T13772 compound mon,mutants
R3822 T13772 T13770 pobj mutants,in
R3823 T13773 T13756 cc and,rescues
R3824 T13774 T13775 aux does,perturb
R3825 T13775 T13756 conj perturb,rescues
R3826 T13776 T13775 neg not,perturb
R3827 T13777 T13775 dobj hematopoiesis,perturb
R3828 T13778 T13775 prep in,perturb
R3829 T13779 T13780 amod wild,type
R3830 T13780 T13782 compound type,embryos
R3831 T13781 T13780 punct -,type
R3832 T13782 T13778 pobj embryos,in
R3833 T13783 T13784 punct (,see
R3834 T13784 T13775 parataxis see,perturb
R3835 T13785 T13784 dobj Figure,see
R3836 T13786 T13785 nummod 5,Figure
R3837 T13787 T13784 punct ),see
R3838 T13788 T13756 punct .,rescues
R3839 T13790 T13791 nsubj We,cloned
R3840 T13792 T13791 aux have,cloned
R3841 T13793 T13791 advmod also,cloned
R3842 T13794 T13795 det the,ortholog
R3843 T13795 T13791 dobj ortholog,cloned
R3844 T13796 T13795 amod predicted,ortholog
R3845 T13797 T13795 compound zebrafish,ortholog
R3846 T13798 T13795 prep of,ortholog
R3847 T13799 T13798 pobj tif1α,of
R3848 T13800 T13795 punct ", ",ortholog
R3849 T13801 T13802 dep which,expressed
R3850 T13802 T13795 relcl expressed,ortholog
R3851 T13803 T13802 auxpass is,expressed
R3852 T13804 T13805 advmod more,uniformly
R3853 T13805 T13802 advmod uniformly,expressed
R3854 T13806 T13802 prep in,expressed
R3855 T13807 T13808 compound zebrafish,embryos
R3856 T13808 T13806 pobj embryos,in
R3857 T13809 T13802 prep like,expressed
R3858 T13810 T13811 amod mammalian,TIF1α
R3859 T13811 T13809 pobj TIF1α,like
R3860 T13812 T13813 punct (,Le
R3861 T13813 T13802 meta Le,expressed
R3862 T13814 T13813 nmod Douarin,Le
R3863 T13815 T13813 nmod et,Le
R3864 T13816 T13813 nmod al.,Le
R3865 T13817 T13813 nummod 1995,Le
R3866 T13818 T13813 punct ;,Le
R3867 T13819 T13813 nmod Niederreither,Le
R3868 T13820 T13813 nmod et,Le
R3869 T13821 T13813 nmod al.,Le
R3870 T13822 T13813 nummod 1999,Le
R3871 T13823 T13813 punct ),Le
R3872 T13824 T13825 punct (,see
R3873 T13825 T13802 parataxis see,expressed
R3874 T13826 T13827 nmod Figures,3A
R3875 T13827 T13825 dobj 3A,see
R3876 T13828 T13827 cc and,3A
R3877 T13829 T13827 conj 4A,3A
R3878 T13830 T13825 punct ),see
R3879 T13831 T13802 cc and,expressed
R3880 T13832 T13833 aux may,be
R3881 T13833 T13802 conj be,expressed
R3882 T13834 T13833 advmod therefore,be
R3883 T13835 T13833 acomp available,be
R3884 T13836 T13837 aux to,form
R3885 T13837 T13833 advcl form,be
R3886 T13838 T13837 dobj hetero-oligomers,form
R3887 T13839 T13837 prep with,form
R3888 T13840 T13841 compound Tif1γ,protein
R3889 T13841 T13839 pobj protein,with
R3890 T13842 T13837 prep in,form
R3891 T13843 T13844 amod developing,cells
R3892 T13844 T13842 pobj cells,in
R3893 T13845 T13844 amod hematopoietic,cells
R3894 T13846 T13791 punct .,cloned
R3895 T13848 T13849 advcl Comparing,appears
R3896 T13850 T13851 amod available,sequences
R3897 T13851 T13848 dobj sequences,Comparing
R3898 T13852 T13851 nmod zebrafish,sequences
R3899 T13853 T13852 cc and,zebrafish
R3900 T13854 T13852 conj mammalian,zebrafish
R3901 T13855 T13856 npadvmod TIF1,predicted
R3902 T13856 T13851 amod predicted,sequences
R3903 T13857 T13856 punct -,predicted
R3904 T13858 T13859 compound amino,acid
R3905 T13859 T13851 compound acid,sequences
R3906 T13860 T13849 punct ", ",appears
R3907 T13861 T13849 nsubj it,appears
R3908 T13862 T13863 mark that,are
R3909 T13863 T13849 ccomp are,appears
R3910 T13864 T13865 det the,orthologs
R3911 T13865 T13863 nsubj orthologs,are
R3912 T13866 T13865 compound Tif1γ,orthologs
R3913 T13867 T13868 det the,members
R3914 T13868 T13863 attr members,are
R3915 T13869 T13870 advmod most,conserved
R3916 T13870 T13868 amod conserved,members
R3917 T13871 T13870 advmod highly,conserved
R3918 T13872 T13868 compound family,members
R3919 T13873 T13874 mark while,are
R3920 T13874 T13849 advcl are,appears
R3921 T13875 T13876 det the,sequences
R3922 T13876 T13874 nsubj sequences,are
R3923 T13877 T13876 compound Tif1α,sequences
R3924 T13878 T13874 advmod relatively,are
R3925 T13879 T13880 advmod more,divergent
R3926 T13880 T13874 acomp divergent,are
R3927 T13881 T13849 punct .,appears
R3928 T13883 T13884 nsubj We,found
R3929 T13885 T13884 aux have,found
R3930 T13886 T13884 neg not,found
R3931 T13887 T13888 det a,ortholog
R3932 T13888 T13884 dobj ortholog,found
R3933 T13889 T13888 compound Tif1β,ortholog
R3934 T13890 T13884 punct ", ",found
R3935 T13891 T13892 advmod thus,far
R3936 T13892 T13884 advmod far,found
R3937 T13893 T13884 punct ", ",found
R3938 T13894 T13884 prep in,found
R3939 T13895 T13896 det the,sequences
R3940 T13896 T13894 pobj sequences,in
R3941 T13897 T13896 nmod zebrafish,sequences
R3942 T13898 T13897 cc or,zebrafish
R3943 T13899 T13897 conj fugu,zebrafish
R3944 T13900 T13896 nmod genome,sequences
R3945 T13901 T13900 cc or,genome
R3946 T13902 T13900 conj EST,genome
R3947 T13903 T13884 punct .,found
R3948 T13905 T13906 nsubj It,is
R3949 T13907 T13906 acomp possible,is
R3950 T13908 T13909 mark that,be
R3951 T13909 T13906 ccomp be,is
R3952 T13910 T13909 nsubj Tif1β,be
R3953 T13911 T13909 punct ", ",be
R3954 T13912 T13909 prep like,be
R3955 T13913 T13914 det the,factors
R3956 T13914 T13912 pobj factors,like
R3957 T13915 T13914 compound KRAB,factors
R3958 T13916 T13914 compound domain,factors
R3959 T13917 T13914 compound transcription,factors
R3960 T13918 T13919 nsubj it,binds
R3961 T13919 T13914 advcl binds,factors
R3962 T13920 T13919 prep to,binds
R3963 T13921 T13909 punct ", ",be
R3964 T13922 T13909 aux may,be
R3965 T13923 T13909 acomp present,be
R3966 T13924 T13925 advmod only,in
R3967 T13925 T13909 prep in,be
R3968 T13926 T13925 pobj tetrapods,in
R3969 T13927 T13928 punct (,Urrutia
R3970 T13928 T13906 meta Urrutia,is
R3971 T13929 T13928 nummod 2003,Urrutia
R3972 T13930 T13928 punct ),Urrutia
R3973 T13931 T13906 punct .,is
R3974 T13933 T13934 advmod However,needed
R3975 T13935 T13934 punct ", ",needed
R3976 T13936 T13937 advmod more,complete
R3977 T13937 T13938 amod complete,sequences
R3978 T13938 T13934 nsubjpass sequences,needed
R3979 T13939 T13938 compound genome,sequences
R3980 T13940 T13934 aux will,needed
R3981 T13941 T13934 auxpass be,needed
R3982 T13942 T13943 aux to,confirm
R3983 T13943 T13934 advcl confirm,needed
R3984 T13944 T13945 det this,hypothesis
R3985 T13945 T13943 dobj hypothesis,confirm
R3986 T13946 T13934 punct .,needed
R3987 T13948 T13949 prep Based,is
R3988 T13950 T13948 prep on,Based
R3989 T13951 T13952 poss our,analysis
R3990 T13952 T13950 pobj analysis,on
R3991 T13953 T13952 prep of,analysis
R3992 T13954 T13955 compound zebrafish,mutants
R3993 T13955 T13953 pobj mutants,of
R3994 T13956 T13955 compound mon,mutants
R3995 T13957 T13949 punct ", ",is
R3996 T13958 T13949 nsubj it,is
R3997 T13959 T13949 acomp reasonable,is
R3998 T13960 T13961 aux to,predict
R3999 T13961 T13949 xcomp predict,is
R4000 T13962 T13963 mark that,plays
R4001 T13963 T13961 ccomp plays,predict
R4002 T13964 T13963 nsubj Tif1γ,plays
R4003 T13965 T13964 punct ", ",Tif1γ
R4004 T13966 T13967 det the,member
R4005 T13967 T13964 appos member,Tif1γ
R4006 T13968 T13969 advmod most,conserved
R4007 T13969 T13967 amod conserved,member
R4008 T13970 T13969 advmod evolutionarily,conserved
R4009 T13971 T13967 compound TIF1,member
R4010 T13972 T13967 compound family,member
R4011 T13973 T13963 punct ", ",plays
R4012 T13974 T13975 det a,role
R4013 T13975 T13963 dobj role,plays
R4014 T13976 T13975 advmod similarly,role
R4015 T13977 T13975 amod essential,role
R4016 T13978 T13963 prep in,plays
R4017 T13979 T13980 amod human,hematopoiesis
R4018 T13980 T13978 pobj hematopoiesis,in
R4019 T13981 T13979 cc and,human
R4020 T13982 T13979 conj mouse,human
R4021 T13983 T13949 punct .,is
R4022 T15217 T15218 nsubj Mutations,Cause
R4023 T15219 T15217 prep in,Mutations
R4024 T15220 T15219 pobj tif1γ,in
R4025 T15221 T15218 dobj Apoptosis,Cause
R4026 T15222 T15221 prep of,Apoptosis
R4027 T15223 T15224 amod Erythroid,Progenitors
R4028 T15224 T15222 pobj Progenitors,of
R4029 T15226 T15227 poss Our,examination
R4030 T15227 T15228 nsubj examination,demonstrates
R4031 T15229 T15227 prep of,examination
R4032 T15230 T15231 amod hematopoietic,expression
R4033 T15231 T15229 pobj expression,of
R4034 T15232 T15231 compound gene,expression
R4035 T15233 T15231 punct ", ",expression
R4036 T15234 T15231 conj apoptosis,expression
R4037 T15235 T15234 punct ", ",apoptosis
R4038 T15236 T15234 cc and,apoptosis
R4039 T15237 T15238 compound marrow,histology
R4040 T15238 T15234 conj histology,apoptosis
R4041 T15239 T15231 prep in,expression
R4042 T15240 T15241 compound mon,mutants
R4043 T15241 T15239 pobj mutants,in
R4044 T15242 T15243 mark that,formed
R4045 T15243 T15228 ccomp formed,demonstrates
R4046 T15244 T15245 amod early,progenitors
R4047 T15245 T15243 nsubjpass progenitors,formed
R4048 T15246 T15245 amod erythroid,progenitors
R4049 T15247 T15243 auxpass are,formed
R4050 T15248 T15243 prep in,formed
R4051 T15249 T15250 amod homozygous,mutants
R4052 T15250 T15248 pobj mutants,in
R4053 T15251 T15228 punct ", ",demonstrates
R4054 T15252 T15228 cc but,demonstrates
R4055 T15253 T15254 nsubj they,fail
R4056 T15254 T15228 conj fail,demonstrates
R4057 T15255 T15256 aux to,differentiate
R4058 T15256 T15254 xcomp differentiate,fail
R4059 T15257 T15256 advmod properly,differentiate
R4060 T15258 T15254 cc and,fail
R4061 T15259 T15260 advmod instead,undergo
R4062 T15260 T15254 conj undergo,fail
R4063 T15261 T15262 amod programmed,death
R4064 T15262 T15260 dobj death,undergo
R4065 T15263 T15262 compound cell,death
R4066 T15264 T15265 punct (,see
R4067 T15265 T15254 parataxis see,fail
R4068 T15266 T15265 dobj Figure,see
R4069 T15267 T15266 nummod 1,Figure
R4070 T15268 T15265 punct ),see
R4071 T15269 T15254 punct .,fail
R4072 T15271 T15272 det The,expression
R4073 T15272 T15273 nsubj expression,appears
R4074 T15274 T15272 prep of,expression
R4075 T15275 T15274 pobj gata1,of
R4076 T15276 T15277 aux to,initiate
R4077 T15277 T15273 xcomp initiate,appears
R4078 T15278 T15277 advmod normally,initiate
R4079 T15279 T15277 prep in,initiate
R4080 T15280 T15281 det the,cells
R4081 T15281 T15279 pobj cells,in
R4082 T15282 T15281 amod committed,cells
R4083 T15283 T15281 amod erythroid,cells
R4084 T15284 T15281 prep of,cells
R4085 T15285 T15286 compound mon,mutants
R4086 T15286 T15284 pobj mutants,of
R4087 T15287 T15273 punct .,appears
R4088 T15289 T15290 advmod However,are
R4089 T15291 T15290 punct ", ",are
R4090 T15292 T15293 det the,cells
R4091 T15293 T15290 nsubj cells,are
R4092 T15294 T15290 acomp abnormal,are
R4093 T15295 T15296 amod prior,to
R4094 T15296 T15290 prep to,are
R4095 T15297 T15298 det the,loss
R4096 T15298 T15296 pobj loss,to
R4097 T15299 T15298 amod complete,loss
R4098 T15300 T15298 prep of,loss
R4099 T15301 T15302 compound gata1,expression
R4100 T15302 T15300 pobj expression,of
R4101 T15303 T15290 punct .,are
R4102 T15305 T15306 npadvmod TUNEL,positive
R4103 T15306 T15308 amod positive,cells
R4104 T15307 T15306 punct -,positive
R4105 T15308 T15310 nsubj cells,are
R4106 T15309 T15308 amod apoptotic,cells
R4107 T15311 T15310 acomp abundant,are
R4108 T15312 T15310 prep by,are
R4109 T15313 T15314 det the,stage
R4110 T15314 T15312 pobj stage,by
R4111 T15315 T15316 nummod 12,somite
R4112 T15316 T15314 compound somite,stage
R4113 T15317 T15316 punct -,somite
R4114 T15318 T15314 prep of,stage
R4115 T15319 T15318 pobj development,of
R4116 T15320 T15310 punct ", ",are
R4117 T15321 T15310 cc and,are
R4118 T15322 T15323 prep by,extinguished
R4119 T15323 T15310 conj extinguished,are
R4120 T15324 T15325 nummod 22,hpf
R4121 T15325 T15322 pobj hpf,by
R4122 T15326 T15327 det all,expression
R4123 T15327 T15323 nsubjpass expression,extinguished
R4124 T15328 T15327 amod hematopoietic,expression
R4125 T15329 T15327 compound gene,expression
R4126 T15330 T15323 auxpass is,extinguished
R4127 T15331 T15323 punct .,extinguished
R4128 T15333 T15334 det The,expression
R4129 T15334 T15335 nsubjpass expression,detected
R4130 T15336 T15334 prep of,expression
R4131 T15337 T15338 compound marker,genes
R4132 T15338 T15336 pobj genes,of
R4133 T15339 T15338 punct ", ",genes
R4134 T15340 T15338 prep including,genes
R4135 T15341 T15340 pobj scl,including
R4136 T15342 T15341 cc and,scl
R4137 T15343 T15341 conj gata2,scl
R4138 T15344 T15341 punct ", ",scl
R4139 T15345 T15341 amod characteristic,scl
R4140 T15346 T15345 prep of,characteristic
R4141 T15347 T15348 amod hematopoietic,cells
R4142 T15348 T15346 pobj cells,of
R4143 T15349 T15348 compound stem,cells
R4144 T15350 T15348 cc and,cells
R4145 T15351 T15352 amod primitive,progenitors
R4146 T15352 T15348 conj progenitors,cells
R4147 T15353 T15352 amod hematopoietic,progenitors
R4148 T15354 T15335 punct ", ",detected
R4149 T15355 T15335 auxpass are,detected
R4150 T15356 T15335 advmod also,detected
R4151 T15357 T15335 neg not,detected
R4152 T15358 T15335 prep in,detected
R4153 T15359 T15360 det the,islands
R4154 T15360 T15358 pobj islands,in
R4155 T15361 T15360 amod embryonic,islands
R4156 T15362 T15360 compound blood,islands
R4157 T15363 T15360 prep of,islands
R4158 T15364 T15363 pobj mutants,of
R4159 T15365 T15335 prep at,detected
R4160 T15366 T15367 nummod 22,hpf
R4161 T15367 T15365 pobj hpf,at
R4162 T15368 T15335 punct .,detected
R4163 T15370 T15371 nsubj This,indicates
R4164 T15372 T15373 mark that,blocked
R4165 T15373 T15371 ccomp blocked,indicates
R4166 T15374 T15375 det the,cells
R4167 T15375 T15373 nsubjpass cells,blocked
R4168 T15376 T15375 amod mutant,cells
R4169 T15377 T15375 amod hematopoietic,cells
R4170 T15378 T15373 auxpass are,blocked
R4171 T15379 T15373 neg not,blocked
R4172 T15380 T15381 amod prior,to
R4173 T15381 T15373 prep to,blocked
R4174 T15382 T15381 pobj commitment,to
R4175 T15383 T15382 prep to,commitment
R4176 T15384 T15385 det the,lineage
R4177 T15385 T15383 pobj lineage,to
R4178 T15386 T15385 amod erythroid,lineage
R4179 T15387 T15373 punct ", ",blocked
R4180 T15388 T15373 cc but,blocked
R4181 T15389 T15390 advmod instead,develop
R4182 T15390 T15373 conj develop,blocked
R4183 T15391 T15390 prep as,develop
R4184 T15392 T15393 amod abnormal,cells
R4185 T15393 T15391 pobj cells,as
R4186 T15394 T15393 amod erythroid,cells
R4187 T15395 T15390 cc and,develop
R4188 T15396 T15390 conj undergo,develop
R4189 T15397 T15396 dobj apoptosis,undergo
R4190 T15398 T15390 punct ", ",develop
R4191 T15399 T15390 advcl similar,develop
R4192 T15400 T15399 prep to,similar
R4193 T15401 T15402 nmod gata1,cells
R4194 T15402 T15400 pobj cells,to
R4195 T15403 T15401 punct -,gata1
R4196 T15404 T15401 amod deficient,gata1
R4197 T15405 T15402 amod erythroid,cells
R4198 T15406 T15407 punct (,Fujiwara
R4199 T15407 T15371 meta Fujiwara,indicates
R4200 T15408 T15407 nmod et,Fujiwara
R4201 T15409 T15407 nmod al.,Fujiwara
R4202 T15410 T15407 nummod 1996,Fujiwara
R4203 T15411 T15407 punct ;,Fujiwara
R4204 T15412 T15407 nmod Lyons,Fujiwara
R4205 T15413 T15407 nmod et,Fujiwara
R4206 T15414 T15407 nmod al.,Fujiwara
R4207 T15415 T15407 nummod 2002,Fujiwara
R4208 T15416 T15407 punct ),Fujiwara
R4209 T15417 T15371 punct .,indicates
R4210 T15419 T15420 amod Defective,erythropoiesis
R4211 T15420 T15421 nsubjpass erythropoiesis,observed
R4212 T15422 T15420 cc and,erythropoiesis
R4213 T15423 T15424 amod severe,anemia
R4214 T15424 T15420 conj anemia,erythropoiesis
R4215 T15425 T15421 auxpass were,observed
R4216 T15426 T15421 advmod also,observed
R4217 T15427 T15421 prep in,observed
R4218 T15428 T15429 amod rare,adults
R4219 T15429 T15427 pobj adults,in
R4220 T15430 T15429 amod surviving,adults
R4221 T15431 T15432 amod homozygous,mon
R4222 T15432 T15429 compound mon,adults
R4223 T15433 T15432 amod mutant,mon
R4224 T15434 T15421 punct ", ",observed
R4225 T15435 T15421 advcl demonstrating,observed
R4226 T15436 T15437 mark that,required
R4227 T15437 T15435 ccomp required,demonstrating
R4228 T15438 T15437 nsubjpass tif1γ,required
R4229 T15439 T15437 auxpass is,required
R4230 T15440 T15437 advmod also,required
R4231 T15441 T15437 prep in,required
R4232 T15442 T15443 amod definitive,hematopoiesis
R4233 T15443 T15441 pobj hematopoiesis,in
R4234 T15444 T15445 punct (,see
R4235 T15445 T15421 parataxis see,observed
R4236 T15446 T15445 dobj Figure,see
R4237 T15447 T15446 nummod 2,Figure
R4238 T15448 T15445 punct ),see
R4239 T15449 T15421 punct .,observed
R4240 T15451 T15452 det The,phenotypes
R4241 T15452 T15454 nsubj phenotypes,reveal
R4242 T15453 T15452 amod zygotic,phenotypes
R4243 T15455 T15452 prep of,phenotypes
R4244 T15456 T15457 compound mon,mutants
R4245 T15457 T15455 pobj mutants,of
R4246 T15458 T15454 aux may,reveal
R4247 T15459 T15454 neg not,reveal
R4248 T15460 T15461 det the,function
R4249 T15461 T15454 dobj function,reveal
R4250 T15462 T15461 prep of,function
R4251 T15463 T15464 advmod maternally,inherited
R4252 T15464 T15465 amod inherited,Tif1γ
R4253 T15465 T15462 pobj Tif1γ,of
R4254 T15466 T15454 punct .,reveal
R4255 T15468 T15469 advmod Maternally,expressed
R4256 T15469 T15470 amod expressed,Tif1γ
R4257 T15470 T15472 nsubj Tif1γ,play
R4258 T15471 T15470 compound zebrafish,Tif1γ
R4259 T15473 T15472 aux may,play
R4260 T15474 T15472 dobj roles,play
R4261 T15475 T15472 prep in,play
R4262 T15476 T15475 pobj hematopoiesis,in
R4263 T15477 T15476 cc or,hematopoiesis
R4264 T15478 T15479 amod other,aspects
R4265 T15479 T15476 conj aspects,hematopoiesis
R4266 T15480 T15479 prep of,aspects
R4267 T15481 T15480 pobj organogenesis,of
R4268 T15482 T15483 dep that,are
R4269 T15483 T15476 relcl are,hematopoiesis
R4270 T15484 T15483 neg not,are
R4271 T15485 T15483 acomp detectable,are
R4272 T15486 T15483 prep due,are
R4273 T15487 T15486 pcomp to,due
R4274 T15488 T15489 det the,presence
R4275 T15489 T15486 pobj presence,due
R4276 T15490 T15489 prep of,presence
R4277 T15491 T15492 amod wild,type
R4278 T15492 T15494 compound type,mRNA
R4279 T15493 T15492 punct -,type
R4280 T15494 T15490 pobj mRNA,of
R4281 T15495 T15489 prep in,presence
R4282 T15496 T15495 pobj eggs,in
R4283 T15497 T15496 acl laid,eggs
R4284 T15498 T15497 agent by,laid
R4285 T15499 T15500 amod heterozygous,mothers
R4286 T15500 T15498 pobj mothers,by
R4287 T15501 T15472 punct .,play
R4288 T15503 T15504 nsubj Analysis,aid
R4289 T15505 T15503 prep of,Analysis
R4290 T15506 T15507 det the,offspring
R4291 T15507 T15505 pobj offspring,of
R4292 T15508 T15507 prep of,offspring
R4293 T15509 T15510 amod homozygous,mutant
R4294 T15510 T15512 nmod mutant,zebrafish
R4295 T15511 T15510 nmod mon,mutant
R4296 T15512 T15508 pobj zebrafish,of
R4297 T15513 T15512 amod female,zebrafish
R4298 T15514 T15504 aux will,aid
R4299 T15515 T15504 prep in,aid
R4300 T15516 T15515 pcomp defining,in
R4301 T15517 T15518 det the,function
R4302 T15518 T15516 dobj function,defining
R4303 T15519 T15518 prep of,function
R4304 T15520 T15521 det this,mRNA
R4305 T15521 T15519 pobj mRNA,of
R4306 T15522 T15521 amod maternal,mRNA
R4307 T15523 T15504 punct .,aid
R4308 T15525 T15526 det The,analysis
R4309 T15526 T15528 nsubj analysis,provides
R4310 T15527 T15526 amod present,analysis
R4311 T15529 T15526 prep of,analysis
R4312 T15530 T15531 amod zygotic,mutants
R4313 T15531 T15529 pobj mutants,of
R4314 T15532 T15531 compound mon,mutants
R4315 T15533 T15528 dobj data,provides
R4316 T15534 T15535 dep that,are
R4317 T15535 T15533 relcl are,data
R4318 T15536 T15535 acomp consistent,are
R4319 T15537 T15536 prep with,consistent
R4320 T15538 T15539 det the,conclusion
R4321 T15539 T15537 pobj conclusion,with
R4322 T15540 T15541 mark that,is
R4323 T15541 T15539 acl is,conclusion
R4324 T15542 T15541 nsubj tif1γ,is
R4325 T15543 T15541 acomp essential,is
R4326 T15544 T15541 prep for,is
R4327 T15545 T15544 pobj erythropoiesis,for
R4328 T15546 T15541 cc but,is
R4329 T15547 T15548 aux do,rule
R4330 T15548 T15541 conj rule,is
R4331 T15549 T15548 neg not,rule
R4332 T15550 T15548 prt out,rule
R4333 T15551 T15552 amod essential,functions
R4334 T15552 T15548 dobj functions,rule
R4335 T15553 T15552 prep in,functions
R4336 T15554 T15555 amod other,lineages
R4337 T15555 T15553 pobj lineages,in
R4338 T15556 T15555 amod hematopoietic,lineages
R4339 T15557 T15528 punct .,provides
R4340 T15559 T15560 det The,phenotype
R4341 T15560 T15562 nsubj phenotype,resembles
R4342 T15561 T15560 amod hematopoietic,phenotype
R4343 T15563 T15560 prep of,phenotype
R4344 T15564 T15565 compound mon,mutants
R4345 T15565 T15563 pobj mutants,of
R4346 T15566 T15567 det the,loss
R4347 T15567 T15562 dobj loss,resembles
R4348 T15568 T15567 prep of,loss
R4349 T15569 T15570 amod erythroid,cells
R4350 T15570 T15568 pobj cells,of
R4351 T15571 T15567 acl seen,loss
R4352 T15572 T15571 prep in,seen
R4353 T15573 T15574 preconj both,embryos
R4354 T15574 T15572 pobj embryos,in
R4355 T15575 T15576 compound mouse,knockout
R4356 T15576 T15574 compound knockout,embryos
R4357 T15577 T15576 compound Gata1,knockout
R4358 T15578 T15574 cc and,embryos
R4359 T15579 T15580 nmod zebrafish,tepes
R4360 T15580 T15582 nmod tepes,embryos
R4361 T15581 T15580 nmod vlad,tepes
R4362 T15582 T15574 conj embryos,embryos
R4363 T15583 T15580 punct (,tepes
R4364 T15584 T15580 appos gata1,tepes
R4365 T15585 T15582 punct ),embryos
R4366 T15586 T15582 amod mutant,embryos
R4367 T15587 T15588 punct (,Fujiwara
R4368 T15588 T15562 meta Fujiwara,resembles
R4369 T15589 T15588 nmod et,Fujiwara
R4370 T15590 T15588 nmod al.,Fujiwara
R4371 T15591 T15588 nummod 1996,Fujiwara
R4372 T15592 T15588 punct ;,Fujiwara
R4373 T15593 T15588 nmod Lyons,Fujiwara
R4374 T15594 T15588 nmod et,Fujiwara
R4375 T15595 T15588 nmod al.,Fujiwara
R4376 T15596 T15588 nummod 2002,Fujiwara
R4377 T15597 T15588 punct ),Fujiwara
R4378 T15598 T15562 punct .,resembles
R4379 T15600 T15601 prep In,tested
R4380 T15602 T15603 det an,effort
R4381 T15603 T15600 pobj effort,In
R4382 T15604 T15605 aux to,determine
R4383 T15605 T15603 acl determine,effort
R4384 T15606 T15607 mark if,is
R4385 T15607 T15605 ccomp is,determine
R4386 T15608 T15607 expl there,is
R4387 T15609 T15610 det a,relationship
R4388 T15610 T15607 attr relationship,is
R4389 T15611 T15610 amod genetic,relationship
R4390 T15612 T15610 prep between,relationship
R4391 T15613 T15612 pobj mon,between
R4392 T15614 T15613 cc and,mon
R4393 T15615 T15613 conj gata1,mon
R4394 T15616 T15601 punct ", ",tested
R4395 T15617 T15601 nsubj we,tested
R4396 T15618 T15619 poss their,ability
R4397 T15619 T15601 dobj ability,tested
R4398 T15620 T15621 aux to,rescue
R4399 T15621 T15619 acl rescue,ability
R4400 T15622 T15621 dobj erythropoiesis,rescue
R4401 T15623 T15601 punct .,tested
R4402 T15625 T15626 preconj Both,injection
R4403 T15626 T15627 nsubj injection,failed
R4404 T15628 T15626 prep of,injection
R4405 T15629 T15630 compound gata1,mRNA
R4406 T15630 T15628 pobj mRNA,of
R4407 T15631 T15626 prep into,injection
R4408 T15632 T15633 npadvmod mon,homozygous
R4409 T15633 T15634 amod homozygous,embryos
R4410 T15634 T15631 pobj embryos,into
R4411 T15635 T15634 compound mutant,embryos
R4412 T15636 T15626 cc and,injection
R4413 T15637 T15626 conj injection,injection
R4414 T15638 T15637 prep of,injection
R4415 T15639 T15640 compound tif1γ,mRNA
R4416 T15640 T15638 pobj mRNA,of
R4417 T15641 T15637 prep into,injection
R4418 T15642 T15643 nmod gata1,embryos
R4419 T15643 T15641 pobj embryos,into
R4420 T15644 T15643 amod knock,embryos
R4421 T15645 T15644 punct -,knock
R4422 T15646 T15644 prt down,knock
R4423 T15647 T15648 aux to,rescue
R4424 T15648 T15627 xcomp rescue,failed
R4425 T15649 T15648 dobj hematopoiesis,rescue
R4426 T15650 T15651 punct (,data
R4427 T15651 T15627 parataxis data,failed
R4428 T15652 T15651 amod unpublished,data
R4429 T15653 T15651 punct ),data
R4430 T15654 T15627 punct .,failed
R4431 T15656 T15657 nsubj We,tested
R4432 T15658 T15657 advmod also,tested
R4433 T15659 T15657 prep for,tested
R4434 T15660 T15661 det a,interaction
R4435 T15661 T15659 pobj interaction,for
R4436 T15662 T15661 amod direct,interaction
R4437 T15663 T15661 prep between,interaction
R4438 T15664 T15665 nmod Tif1γ,proteins
R4439 T15665 T15663 pobj proteins,between
R4440 T15666 T15664 cc and,Tif1γ
R4441 T15667 T15664 conj Gata1,Tif1γ
R4442 T15668 T15657 prep by,tested
R4443 T15669 T15670 nmod coimmunoprecipitation,assays
R4444 T15670 T15668 pobj assays,by
R4445 T15671 T15669 cc and,coimmunoprecipitation
R4446 T15672 T15673 nmod yeast,hybrid
R4447 T15673 T15669 conj hybrid,coimmunoprecipitation
R4448 T15674 T15673 nummod two,hybrid
R4449 T15675 T15673 punct -,hybrid
R4450 T15676 T15657 cc and,tested
R4451 T15677 T15657 conj found,tested
R4452 T15678 T15679 det no,association
R4453 T15679 T15677 dobj association,found
R4454 T15680 T15681 punct (,data
R4455 T15681 T15677 parataxis data,found
R4456 T15682 T15681 amod unpublished,data
R4457 T15683 T15681 punct ),data
R4458 T15684 T15657 punct .,tested
R4459 T15686 T15687 mark Although,arrest
R4460 T15687 T15695 advcl arrest,suggest
R4461 T15688 T15689 det the,mutations
R4462 T15689 T15687 nsubj mutations,arrest
R4463 T15690 T15689 prep in,mutations
R4464 T15691 T15690 pobj each,in
R4465 T15692 T15691 prep of,each
R4466 T15693 T15694 det these,genes
R4467 T15694 T15692 pobj genes,of
R4468 T15696 T15687 dobj cells,arrest
R4469 T15697 T15687 prep at,arrest
R4470 T15698 T15699 det a,stage
R4471 T15699 T15697 pobj stage,at
R4472 T15700 T15699 amod similar,stage
R4473 T15701 T15699 prep of,stage
R4474 T15702 T15701 pobj development,of
R4475 T15703 T15695 punct ", ",suggest
R4476 T15704 T15705 poss our,results
R4477 T15705 T15695 nsubj results,suggest
R4478 T15706 T15707 mark that,act
R4479 T15707 T15695 ccomp act,suggest
R4480 T15708 T15707 nsubj gata1,act
R4481 T15709 T15708 cc and,gata1
R4482 T15710 T15708 conj tif1γ,gata1
R4483 T15711 T15707 advmod independently,act
R4484 T15712 T15695 punct .,suggest
R4485 T15714 T15715 nsubj This,rule
R4486 T15716 T15715 aux does,rule
R4487 T15717 T15715 neg not,rule
R4488 T15718 T15715 prt out,rule
R4489 T15719 T15720 det the,possibility
R4490 T15720 T15715 dobj possibility,rule
R4491 T15721 T15722 mark that,regulate
R4492 T15722 T15720 acl regulate,possibility
R4493 T15723 T15724 amod parallel,pathways
R4494 T15724 T15722 nsubj pathways,regulate
R4495 T15725 T15724 amod genetic,pathways
R4496 T15726 T15724 acl involving,pathways
R4497 T15727 T15726 dobj gata1,involving
R4498 T15728 T15727 cc and,gata1
R4499 T15729 T15727 conj tif1γ,gata1
R4500 T15730 T15722 punct ", ",regulate
R4501 T15731 T15722 advcl operating,regulate
R4502 T15732 T15731 advmod together,operating
R4503 T15733 T15722 punct ", ",regulate
R4504 T15734 T15735 compound gene,transcription
R4505 T15735 T15722 dobj transcription,regulate
R4506 T15736 T15722 prep within,regulate
R4507 T15737 T15738 compound blood,cells
R4508 T15738 T15736 pobj cells,within
R4509 T15739 T15715 punct .,rule
R4515 T16380 T16381 det The,Role
R4516 T16382 T16381 prep of,Role
R4517 T16383 T16382 pobj Tif1γ,of
R4518 T16384 T16381 prep in,Role
R4519 T16385 T16386 amod Primitive,Erythropoiesis
R4520 T16386 T16384 pobj Erythropoiesis,in
R4521 T16387 T16385 cc and,Primitive
R4522 T16388 T16385 conj Definitive,Primitive
R4523 T16390 T16391 advcl Taken,suggest
R4524 T16392 T16390 advmod together,Taken
R4525 T16393 T16391 punct ", ",suggest
R4526 T16394 T16395 poss our,data
R4527 T16395 T16391 nsubj data,suggest
R4528 T16396 T16397 mark that,required
R4529 T16397 T16391 ccomp required,suggest
R4530 T16398 T16397 nsubjpass tif1γ,required
R4531 T16399 T16397 auxpass is,required
R4532 T16400 T16397 prep as,required
R4533 T16401 T16402 det a,cofactor
R4534 T16402 T16400 pobj cofactor,as
R4535 T16403 T16402 amod permissive,cofactor
R4536 T16404 T16397 prep for,required
R4537 T16405 T16406 det the,control
R4538 T16406 T16404 pobj control,for
R4539 T16407 T16406 amod erythroid,control
R4540 T16408 T16409 npadvmod lineage,specific
R4541 T16409 T16406 amod specific,control
R4542 T16410 T16409 punct -,specific
R4543 T16411 T16406 prep of,control
R4544 T16412 T16413 amod hematopoietic,gene
R4545 T16413 T16414 compound gene,expression
R4546 T16414 T16411 pobj expression,of
R4547 T16415 T16391 punct .,suggest
R4548 T16417 T16418 nsubj We,predict
R4549 T16419 T16418 advmod reasonably,predict
R4550 T16420 T16421 mark that,functions
R4551 T16421 T16418 ccomp functions,predict
R4552 T16422 T16423 compound Tif1γ,protein
R4553 T16423 T16421 nsubj protein,functions
R4554 T16424 T16421 prep as,functions
R4555 T16425 T16426 det a,factor
R4556 T16426 T16424 pobj factor,as
R4557 T16427 T16426 amod transcriptional,factor
R4558 T16428 T16426 amod intermediary,factor
R4559 T16429 T16421 prep in,functions
R4560 T16430 T16431 amod hematopoietic,cells
R4561 T16431 T16429 pobj cells,in
R4562 T16432 T16431 compound progenitor,cells
R4563 T16433 T16421 prep given,functions
R4564 T16434 T16435 mark that,shown
R4565 T16435 T16433 pcomp shown,given
R4566 T16436 T16437 preconj both,TIF1α
R4567 T16437 T16435 nsubjpass TIF1α,shown
R4568 T16438 T16439 punct (,Zhong
R4569 T16439 T16437 meta Zhong,TIF1α
R4570 T16440 T16439 nmod et,Zhong
R4571 T16441 T16439 nmod al.,Zhong
R4572 T16442 T16439 nummod 1999,Zhong
R4573 T16443 T16439 punct ),Zhong
R4574 T16444 T16437 cc and,TIF1α
R4575 T16445 T16437 conj TIF1β,TIF1α
R4576 T16446 T16447 punct (,Friedman
R4577 T16447 T16445 meta Friedman,TIF1β
R4578 T16448 T16447 nmod et,Friedman
R4579 T16449 T16447 nmod al.,Friedman
R4580 T16450 T16447 nummod 1996,Friedman
R4581 T16451 T16447 punct ;,Friedman
R4582 T16452 T16447 nmod Abrink,Friedman
R4583 T16453 T16447 nmod et,Friedman
R4584 T16454 T16447 nmod al.,Friedman
R4585 T16455 T16447 nummod 2001,Friedman
R4586 T16456 T16447 punct ),Friedman
R4587 T16457 T16435 aux have,shown
R4588 T16458 T16435 auxpass been,shown
R4589 T16459 T16460 aux to,act
R4590 T16460 T16435 xcomp act,shown
R4591 T16461 T16460 prep as,act
R4592 T16462 T16463 amod intermediary,factors
R4593 T16463 T16461 pobj factors,as
R4594 T16464 T16465 dep that,regulate
R4595 T16465 T16463 relcl regulate,factors
R4596 T16466 T16465 advmod positively,regulate
R4597 T16467 T16466 cc or,positively
R4598 T16468 T16466 conj negatively,positively
R4599 T16469 T16470 compound gene,transcription
R4600 T16470 T16465 dobj transcription,regulate
R4601 T16471 T16418 punct .,predict
R4602 T16473 T16474 det These,studies
R4603 T16474 T16475 nsubj studies,indicate
R4604 T16476 T16477 mark that,act
R4605 T16477 T16475 ccomp act,indicate
R4606 T16478 T16477 nsubj TIF1α,act
R4607 T16479 T16478 cc and,TIF1α
R4608 T16480 T16478 conj TIF1β,TIF1α
R4609 T16481 T16477 prep as,act
R4610 T16482 T16481 pobj scaffolds,as
R4611 T16483 T16484 dep that,link
R4612 T16484 T16482 relcl link,scaffolds
R4613 T16485 T16486 amod different,classes
R4614 T16486 T16484 dobj classes,link
R4615 T16487 T16486 prep of,classes
R4616 T16488 T16489 npadvmod DNA,binding
R4617 T16489 T16491 amod binding,proteins
R4618 T16490 T16489 punct -,binding
R4619 T16491 T16487 pobj proteins,of
R4620 T16492 T16491 cc and,proteins
R4621 T16493 T16494 npadvmod chromatin,associated
R4622 T16494 T16496 amod associated,proteins
R4623 T16495 T16494 punct -,associated
R4624 T16496 T16491 conj proteins,proteins
R4625 T16497 T16484 prep into,link
R4626 T16498 T16499 amod larger,complexes
R4627 T16499 T16497 pobj complexes,into
R4628 T16500 T16499 amod regulatory,complexes
R4629 T16501 T16475 punct .,indicate
R4630 T16503 T16504 nsubjpass Tif1γ,detected
R4631 T16505 T16504 auxpass is,detected
R4632 T16506 T16504 prep within,detected
R4633 T16507 T16508 amod nuclear,foci
R4634 T16508 T16506 pobj foci,within
R4635 T16509 T16510 punct (,see
R4636 T16510 T16508 parataxis see,foci
R4637 T16511 T16510 dobj Figure,see
R4638 T16512 T16511 nummod 6,Figure
R4639 T16513 T16510 punct ),see
R4640 T16514 T16508 punct ", ",foci
R4641 T16515 T16516 dep which,appear
R4642 T16516 T16508 relcl appear,foci
R4643 T16517 T16516 punct ", ",appear
R4644 T16518 T16516 prep based,appear
R4645 T16519 T16518 prep on,based
R4646 T16520 T16521 poss our,analysis
R4647 T16521 T16519 pobj analysis,on
R4648 T16522 T16516 punct ", ",appear
R4649 T16523 T16516 aux do,appear
R4650 T16524 T16516 neg not,appear
R4651 T16525 T16526 aux to,correspond
R4652 T16526 T16516 xcomp correspond,appear
R4653 T16527 T16526 prep to,correspond
R4654 T16528 T16529 amod several,types
R4655 T16529 T16527 pobj types,to
R4656 T16530 T16529 prep of,types
R4657 T16531 T16532 advmod previously,described
R4658 T16532 T16533 amod described,bodies
R4659 T16533 T16530 pobj bodies,of
R4660 T16534 T16533 amod nuclear,bodies
R4661 T16535 T16533 punct ", ",bodies
R4662 T16536 T16533 prep including,bodies
R4663 T16537 T16538 compound PML,bodies
R4664 T16538 T16536 pobj bodies,including
R4665 T16539 T16504 punct .,detected
R4666 T16541 T16542 nsubjpass Localization,regulated
R4667 T16543 T16541 prep of,Localization
R4668 T16544 T16543 pobj Tif1γ,of
R4669 T16545 T16541 prep to,Localization
R4670 T16546 T16547 det these,bodies
R4671 T16547 T16545 pobj bodies,to
R4672 T16548 T16547 amod nuclear,bodies
R4673 T16549 T16542 aux may,regulated
R4674 T16550 T16542 auxpass be,regulated
R4675 T16551 T16542 agent by,regulated
R4676 T16552 T16553 amod posttranslational,modification
R4677 T16553 T16551 pobj modification,by
R4678 T16554 T16555 amod such,as
R4679 T16555 T16553 prep as,modification
R4680 T16556 T16557 compound SUMO,modification
R4681 T16557 T16555 pobj modification,as
R4682 T16558 T16559 dep that,required
R4683 T16559 T16553 relcl required,modification
R4684 T16560 T16559 auxpass is,required
R4685 T16561 T16562 mark for,form
R4686 T16562 T16559 advcl form,required
R4687 T16563 T16562 nsubj PML,form
R4688 T16564 T16562 aux to,form
R4689 T16565 T16566 nmod PML,domains
R4690 T16566 T16562 dobj domains,form
R4691 T16567 T16566 amod nuclear,domains
R4692 T16568 T16569 punct (,Zhong
R4693 T16569 T16542 meta Zhong,regulated
R4694 T16570 T16569 nmod et,Zhong
R4695 T16571 T16569 nmod al.,Zhong
R4696 T16572 T16569 nummod 2000a,Zhong
R4697 T16573 T16569 punct ", ",Zhong
R4698 T16574 T16569 nummod 2000b,Zhong
R4699 T16575 T16569 punct ;,Zhong
R4700 T16576 T16569 nmod Best,Zhong
R4701 T16577 T16569 nmod et,Zhong
R4702 T16578 T16569 nmod al.,Zhong
R4703 T16579 T16569 nummod 2002,Zhong
R4704 T16580 T16569 punct ),Zhong
R4705 T16581 T16542 punct .,regulated
R4706 T16583 T16584 det These,foci
R4707 T16584 T16585 nsubj foci,serve
R4708 T16586 T16585 aux may,serve
R4709 T16587 T16585 prep as,serve
R4710 T16588 T16589 compound assembly,points
R4711 T16589 T16587 pobj points,as
R4712 T16590 T16591 advmod where,forms
R4713 T16591 T16589 relcl forms,points
R4714 T16592 T16591 nsubj Tif1γ,forms
R4715 T16593 T16594 amod multisubunit,complexes
R4716 T16594 T16591 dobj complexes,forms
R4717 T16595 T16591 prep with,forms
R4718 T16596 T16597 nmod DNA,factors
R4719 T16597 T16595 pobj factors,with
R4720 T16598 T16596 punct -,DNA
R4721 T16599 T16596 amod binding,DNA
R4722 T16600 T16597 compound transcription,factors
R4723 T16601 T16597 cc and,factors
R4724 T16602 T16603 poss their,coactivators
R4725 T16603 T16597 conj coactivators,factors
R4726 T16604 T16603 amod other,coactivators
R4727 T16605 T16603 amod essential,coactivators
R4728 T16606 T16603 cc or,coactivators
R4729 T16607 T16603 conj corepressors,coactivators
R4730 T16608 T16591 punct ", ",forms
R4731 T16609 T16591 prep during,forms
R4732 T16610 T16611 det the,stages
R4733 T16611 T16609 pobj stages,during
R4734 T16612 T16611 amod early,stages
R4735 T16613 T16611 prep of,stages
R4736 T16614 T16615 amod erythroid,differentiation
R4737 T16615 T16613 pobj differentiation,of
R4738 T16616 T16585 punct .,serve
R4739 T16618 T16619 nsubj It,be
R4740 T16620 T16619 aux will,be
R4741 T16621 T16619 acomp important,be
R4742 T16622 T16623 aux to,determine
R4743 T16623 T16619 xcomp determine,be
R4744 T16624 T16625 det the,identity
R4745 T16625 T16623 dobj identity,determine
R4746 T16626 T16625 prep of,identity
R4747 T16627 T16628 npadvmod Tif1γ,interacting
R4748 T16628 T16630 amod interacting,proteins
R4749 T16629 T16628 punct -,interacting
R4750 T16630 T16626 pobj proteins,of
R4751 T16631 T16623 prep in,determine
R4752 T16632 T16633 amod nuclear,foci
R4753 T16633 T16631 pobj foci,in
R4754 T16634 T16623 cc and,determine
R4755 T16635 T16623 conj establish,determine
R4756 T16636 T16637 advmod how,function
R4757 T16637 T16635 ccomp function,establish
R4758 T16638 T16637 nsubj they,function
R4759 T16639 T16637 prep with,function
R4760 T16640 T16639 pobj Tif1γ,with
R4761 T16641 T16642 aux to,regulate
R4762 T16642 T16637 advcl regulate,function
R4763 T16643 T16644 compound blood,cell
R4764 T16644 T16645 compound cell,development
R4765 T16645 T16642 dobj development,regulate
R4766 T16646 T16619 punct .,be
R4767 T17034 T17033 cc and,Zebrafish
R4768 T17035 T17033 conj mouse,Zebrafish
R4769 T17036 T17033 appos strains,Zebrafish
R4770 T17037 T17036 cc and,strains
R4771 T17038 T17036 conj studies,strains
R4772 T17040 T17041 nsubjpass Zebrafish,maintained
R4773 T17042 T17041 auxpass were,maintained
R4774 T17043 T17041 cc and,maintained
R4775 T17044 T17041 conj staged,maintained
R4776 T17045 T17046 mark as,described
R4777 T17046 T17044 advcl described,staged
R4778 T17047 T17048 punct (,Westerfield
R4779 T17048 T17044 meta Westerfield,staged
R4780 T17049 T17048 nummod 1998,Westerfield
R4781 T17050 T17048 punct ),Westerfield
R4782 T17051 T17041 punct .,maintained
R4783 T17053 T17054 det The,alleles
R4784 T17054 T17055 nsubjpass alleles,generated
R4785 T17056 T17054 appos montb222b,alleles
R4786 T17057 T17056 cc and,montb222b
R4787 T17058 T17056 conj montg234,montb222b
R4788 T17059 T17055 auxpass were,generated
R4789 T17060 T17055 prep in,generated
R4790 T17061 T17062 det a,screen
R4791 T17062 T17060 pobj screen,in
R4792 T17063 T17064 amod large,scale
R4793 T17064 T17062 compound scale,screen
R4794 T17065 T17064 punct -,scale
R4795 T17066 T17062 prep for,screen
R4796 T17067 T17068 npadvmod ENU,induced
R4797 T17068 T17070 amod induced,mutations
R4798 T17069 T17068 punct -,induced
R4799 T17070 T17066 pobj mutations,for
R4800 T17071 T17072 punct (,Ransom
R4801 T17072 T17055 meta Ransom,generated
R4802 T17073 T17072 nmod et,Ransom
R4803 T17074 T17072 nmod al.,Ransom
R4804 T17075 T17072 nummod 1996,Ransom
R4805 T17076 T17072 punct ),Ransom
R4806 T17077 T17055 prep on,generated
R4807 T17078 T17079 det the,TU
R4808 T17079 T17077 pobj TU,on
R4809 T17080 T17055 punct ", ",generated
R4810 T17081 T17082 mark whereas,derived
R4811 T17082 T17055 advcl derived,generated
R4812 T17083 T17084 det the,allele
R4813 T17084 T17082 nsubjpass allele,derived
R4814 T17085 T17084 compound monm262,allele
R4815 T17086 T17082 auxpass was,derived
R4816 T17087 T17082 prep on,derived
R4817 T17088 T17089 det the,strain
R4818 T17089 T17087 pobj strain,on
R4819 T17090 T17089 compound AB,strain
R4820 T17091 T17082 cc and,derived
R4821 T17092 T17093 auxpass was,called
R4822 T17093 T17082 conj called,derived
R4823 T17094 T17093 advmod originally,called
R4824 T17095 T17093 oprd vampire,called
R4825 T17096 T17097 punct (,Weinstein
R4826 T17097 T17093 meta Weinstein,called
R4827 T17098 T17097 nmod et,Weinstein
R4828 T17099 T17097 nmod al.,Weinstein
R4829 T17100 T17097 nummod 1996,Weinstein
R4830 T17101 T17097 punct ),Weinstein
R4831 T17102 T17055 punct .,generated
R4832 T17104 T17105 amod Mapping,strains
R4833 T17105 T17106 nsubjpass strains,constructed
R4834 T17107 T17106 auxpass were,constructed
R4835 T17108 T17106 prep by,constructed
R4836 T17109 T17108 pcomp mating,by
R4837 T17110 T17109 prep to,mating
R4838 T17111 T17112 nmod WIK,strains
R4839 T17112 T17110 pobj strains,to
R4840 T17113 T17111 cc or,WIK
R4841 T17114 T17111 conj SJD,WIK
R4842 T17115 T17112 amod polymorphic,strains
R4843 T17116 T17106 punct .,constructed
R4844 T17118 T17119 compound Linkage,analysis
R4845 T17119 T17120 nsubjpass analysis,performed
R4846 T17121 T17120 auxpass was,performed
R4847 T17122 T17120 prep on,performed
R4848 T17123 T17124 amod haploid,embryos
R4849 T17124 T17122 pobj embryos,on
R4850 T17125 T17123 cc or,haploid
R4851 T17126 T17123 conj diploid,haploid
R4852 T17127 T17124 acl obtained,embryos
R4853 T17128 T17127 prep from,obtained
R4854 T17129 T17130 nmod TU,SJD
R4855 T17130 T17132 nmod SJD,hybrids
R4856 T17131 T17130 punct /,SJD
R4857 T17132 T17128 pobj hybrids,from
R4858 T17133 T17130 cc or,SJD
R4859 T17134 T17135 compound TU,WIK
R4860 T17135 T17130 conj WIK,SJD
R4861 T17136 T17135 punct /,WIK
R4862 T17137 T17120 punct .,performed
R4863 T17139 T17140 advmod In,situ
R4864 T17140 T17141 amod situ,hybridization
R4865 T17141 T17142 nsubjpass hybridization,done
R4866 T17143 T17141 cc and,hybridization
R4867 T17144 T17141 conj stainings,hybridization
R4868 T17145 T17141 prep of,hybridization
R4869 T17146 T17145 pobj embryos,of
R4870 T17147 T17142 auxpass were,done
R4871 T17148 T17149 mark as,described
R4872 T17149 T17142 advcl described,done
R4873 T17150 T17151 punct (,Thompson
R4874 T17151 T17142 meta Thompson,done
R4875 T17152 T17151 nmod et,Thompson
R4876 T17153 T17151 nmod al.,Thompson
R4877 T17154 T17151 nummod 1998,Thompson
R4878 T17155 T17151 punct ;,Thompson
R4879 T17156 T17151 nmod Liao,Thompson
R4880 T17157 T17151 nmod et,Thompson
R4881 T17158 T17151 nmod al.,Thompson
R4882 T17159 T17151 nummod 2002,Thompson
R4883 T17160 T17151 punct ),Thompson
R4884 T17161 T17142 punct .,done
R4885 T17163 T17164 advmod In,situ
R4886 T17164 T17165 amod situ,hybridization
R4887 T17165 T17166 nsubjpass hybridization,performed
R4888 T17167 T17165 prep of,hybridization
R4889 T17168 T17169 compound mouse,embryos
R4890 T17169 T17167 pobj embryos,of
R4891 T17170 T17166 auxpass was,performed
R4892 T17171 T17172 mark as,described
R4893 T17172 T17166 advcl described,performed
R4894 T17173 T17174 punct (,Kingsley
R4895 T17174 T17166 meta Kingsley,performed
R4896 T17175 T17174 nmod et,Kingsley
R4897 T17176 T17174 nmod al.,Kingsley
R4898 T17177 T17174 nummod 2001,Kingsley
R4899 T17178 T17174 punct ),Kingsley
R4900 T17179 T17166 punct .,performed
R4901 T17181 T17182 amod Genomic,isolation
R4902 T17182 T17184 nsubjpass isolation,performed
R4903 T17183 T17182 compound DNA,isolation
R4904 T17185 T17182 punct ", ",isolation
R4905 T17186 T17182 conj genotyping,isolation
R4906 T17187 T17186 punct ", ",genotyping
R4907 T17188 T17189 compound AFLP,analysis
R4908 T17189 T17186 conj analysis,genotyping
R4909 T17190 T17189 punct ", ",analysis
R4910 T17191 T17189 cc and,analysis
R4911 T17192 T17193 amod chromosomal,walking
R4912 T17193 T17189 conj walking,analysis
R4913 T17194 T17184 auxpass were,performed
R4914 T17195 T17184 advmod each,performed
R4915 T17196 T17197 mark as,described
R4916 T17197 T17184 advcl described,performed
R4917 T17198 T17197 advmod previously,described
R4918 T17199 T17200 punct (,Brownlie
R4919 T17200 T17184 meta Brownlie,performed
R4920 T17201 T17200 nmod et,Brownlie
R4921 T17202 T17200 nmod al.,Brownlie
R4922 T17203 T17200 nummod 1998,Brownlie
R4923 T17204 T17200 punct ;,Brownlie
R4924 T17205 T17200 conj Ransom,Brownlie
R4925 T17206 T17205 cc and,Ransom
R4926 T17207 T17205 conj Zon,Ransom
R4927 T17208 T17207 nummod 1999,Zon
R4928 T17209 T17207 punct ),Zon
R4929 T17210 T17184 punct .,performed
R4930 T17212 T17213 det A,list
R4931 T17213 T17215 nsubj list,are
R4932 T17214 T17213 amod complete,list
R4933 T17216 T17213 prep of,list
R4934 T17217 T17216 pobj primers,of
R4935 T17218 T17213 prep for,list
R4936 T17219 T17220 amod genetic,mapping
R4937 T17220 T17218 pobj mapping,for
R4938 T17221 T17220 punct ", ",mapping
R4939 T17222 T17223 compound RT,PCR
R4940 T17223 T17220 conj PCR,mapping
R4941 T17224 T17223 punct -,PCR
R4942 T17225 T17223 punct ", ",PCR
R4943 T17226 T17223 cc and,PCR
R4944 T17227 T17223 conj sequencing,PCR
R4945 T17228 T17220 prep of,mapping
R4946 T17229 T17228 pobj mon,of
R4947 T17230 T17215 acomp available,are
R4948 T17231 T17215 prep on,are
R4949 T17232 T17231 pobj request,on
R4950 T17233 T17215 punct .,are
R4951 T17335 T17336 compound mRNA,expression
R4952 T17336 T17337 compound expression,constructs
R4953 T17338 T17337 punct ", ",constructs
R4954 T17339 T17337 conj morpholinos,constructs
R4955 T17340 T17339 punct ", ",morpholinos
R4956 T17341 T17339 cc and,morpholinos
R4957 T17342 T17339 conj microinjection,morpholinos
R4958 T17344 T17345 det The,cDNA
R4959 T17345 T17350 nsubjpass cDNA,subcloned
R4960 T17346 T17347 amod full,length
R4961 T17347 T17345 compound length,cDNA
R4962 T17348 T17347 punct -,length
R4963 T17349 T17345 compound mon,cDNA
R4964 T17351 T17350 auxpass was,subcloned
R4965 T17352 T17350 prep into,subcloned
R4966 T17353 T17354 nmod EcoRI,sites
R4967 T17354 T17352 pobj sites,into
R4968 T17355 T17353 cc and,EcoRI
R4969 T17356 T17353 conj XhoI,EcoRI
R4970 T17357 T17350 prep in,subcloned
R4971 T17358 T17359 det the,vector
R4972 T17359 T17357 pobj vector,in
R4973 T17360 T17359 nmod pCS2,vector
R4974 T17361 T17360 punct +,pCS2
R4975 T17362 T17350 punct .,subcloned
R4976 T17364 T17365 amod Synthetic,mRNA
R4977 T17365 T17366 nsubjpass mRNA,transcribed
R4978 T17367 T17366 auxpass was,transcribed
R4979 T17368 T17369 advmod in,vitro
R4980 T17369 T17366 advmod vitro,transcribed
R4981 T17370 T17366 punct ", ",transcribed
R4982 T17371 T17366 cc and,transcribed
R4983 T17372 T17373 nsubjpass microinjection,performed
R4984 T17373 T17366 conj performed,transcribed
R4985 T17374 T17373 auxpass was,performed
R4986 T17375 T17373 advmod essentially,performed
R4987 T17376 T17377 mark as,described
R4988 T17377 T17373 advcl described,performed
R4989 T17378 T17379 punct (,Liao
R4990 T17379 T17373 meta Liao,performed
R4991 T17380 T17379 nmod et,Liao
R4992 T17381 T17379 nmod al.,Liao
R4993 T17382 T17379 nummod 2002,Liao
R4994 T17383 T17379 punct ),Liao
R4995 T17384 T17373 punct .,performed
R4996 T17642 T17643 compound Cell,transplantation
R4997 T17645 T17646 amod Whole,cells
R4998 T17646 T17649 nsubjpass cells,isolated
R4999 T17647 T17648 compound kidney,marrow
R5000 T17648 T17646 compound marrow,cells
R5001 T17650 T17649 auxpass were,isolated
R5002 T17651 T17649 prep from,isolated
R5003 T17652 T17653 amod adult,gata1
R5004 T17653 T17651 pobj gata1,from
R5005 T17654 T17653 punct :,gata1
R5006 T17655 T17656 nmod EGFP,donors
R5007 T17656 T17653 appos donors,gata1
R5008 T17657 T17656 amod transgenic,donors
R5009 T17658 T17656 punct ", ",donors
R5010 T17659 T17656 acl resuspended,donors
R5011 T17660 T17659 prep in,resuspended
R5012 T17661 T17662 nummod 0.9,saline
R5013 T17662 T17660 pobj saline,in
R5014 T17663 T17661 punct X,0.9
R5015 T17664 T17665 npadvmod phosphate,buffered
R5016 T17665 T17662 amod buffered,saline
R5017 T17666 T17665 punct -,buffered
R5018 T17667 T17662 punct +,saline
R5019 T17668 T17669 nummod 5,%
R5020 T17669 T17670 nmod %,serum
R5021 T17670 T17662 appos serum,saline
R5022 T17671 T17670 amod fetal,serum
R5023 T17672 T17670 amod bovine,serum
R5024 T17673 T17659 punct ", ",resuspended
R5025 T17674 T17659 cc and,resuspended
R5026 T17675 T17659 conj injected,resuspended
R5027 T17676 T17675 prep into,injected
R5028 T17677 T17678 det the,venosus
R5029 T17678 T17676 pobj venosus,into
R5030 T17679 T17678 compound sinus,venosus
R5031 T17680 T17678 prep of,venosus
R5032 T17681 T17682 nummod 2,d
R5033 T17682 T17684 npadvmod d,old
R5034 T17683 T17682 punct -,d
R5035 T17684 T17686 amod old,embryos
R5036 T17685 T17684 punct -,old
R5037 T17686 T17680 pobj embryos,of
R5038 T17687 T17686 nmod montg234,embryos
R5039 T17688 T17687 punct −,montg234
R5040 T17689 T17687 punct /,montg234
R5041 T17690 T17687 punct −,montg234
R5042 T17691 T17687 cc and,montg234
R5043 T17692 T17687 conj control,montg234
R5044 T17693 T17649 punct .,isolated
R5045 T17695 T17696 quantmod Between,102
R5046 T17696 T17697 nummod 102,cells
R5047 T17697 T17702 nsubjpass cells,injected
R5048 T17698 T17696 cc and,102
R5049 T17699 T17696 conj 103,102
R5050 T17700 T17701 compound kidney,marrow
R5051 T17701 T17697 compound marrow,cells
R5052 T17703 T17702 auxpass were,injected
R5053 T17704 T17702 prep per,injected
R5054 T17705 T17704 pobj embryo,per
R5055 T17706 T17702 punct .,injected
R5056 T17708 T17709 amod Individual,embryos
R5057 T17709 T17711 nsubjpass embryos,anesthetized
R5058 T17710 T17709 amod transplanted,embryos
R5059 T17712 T17711 auxpass were,anesthetized
R5060 T17713 T17711 cc and,anesthetized
R5061 T17714 T17711 conj visualized,anesthetized
R5062 T17715 T17714 advmod daily,visualized
R5063 T17716 T17714 prep under,visualized
R5064 T17717 T17718 det an,microscope
R5065 T17718 T17716 pobj microscope,under
R5066 T17719 T17718 amod inverted,microscope
R5067 T17720 T17718 amod fluorescent,microscope
R5068 T17721 T17722 punct (,Leica
R5069 T17722 T17718 parataxis Leica,microscope
R5070 T17723 T17724 compound DM,IRE2
R5071 T17724 T17722 dep IRE2,Leica
R5072 T17725 T17724 punct -,IRE2
R5073 T17726 T17722 punct ;,Leica
R5074 T17727 T17722 punct ", ",Leica
R5075 T17728 T17722 npadvmod Wetzlar,Leica
R5076 T17729 T17722 punct ", ",Leica
R5077 T17730 T17722 npadvmod Germany,Leica
R5078 T17731 T17722 punct ),Leica
R5079 T17732 T17714 prep for,visualized
R5080 T17733 T17734 nmod GFP,cells
R5081 T17734 T17732 pobj cells,for
R5082 T17735 T17734 punct +,cells
R5083 T17736 T17714 prep over,visualized
R5084 T17737 T17738 det a,span
R5085 T17738 T17736 pobj span,over
R5086 T17739 T17738 prep of,span
R5087 T17740 T17741 nummod 12,d
R5088 T17741 T17739 pobj d,of
R5089 T17742 T17711 punct .,anesthetized
R5090 T17744 T17745 prep On,placed
R5091 T17746 T17744 pobj day,On
R5092 T17747 T17746 nummod 13,day
R5093 T17748 T17746 advmod posttransplant,day
R5094 T17749 T17745 punct ", ",placed
R5095 T17750 T17751 det all,larvae
R5096 T17751 T17745 nsubjpass larvae,placed
R5097 T17752 T17751 amod surviving,larvae
R5098 T17753 T17754 punct (,129
R5099 T17754 T17751 parataxis 129,larvae
R5100 T17755 T17754 quantmod 12,129
R5101 T17756 T17754 punct /,129
R5102 T17757 T17754 punct ;,129
R5103 T17758 T17759 nummod 9,%
R5104 T17759 T17754 appos %,129
R5105 T17760 T17754 punct ),129
R5106 T17761 T17745 auxpass were,placed
R5107 T17762 T17745 prep in,placed
R5108 T17763 T17762 pobj tanks,in
R5109 T17764 T17745 cc and,placed
R5110 T17765 T17745 conj monitored,placed
R5111 T17766 T17765 prep for,monitored
R5112 T17767 T17766 pobj survival,for
R5113 T17768 T17745 punct .,placed
R5114 T18183 T18182 punct ", ",Antibodies
R5115 T18184 T18182 conj immunostaining,Antibodies
R5116 T18185 T18184 punct ", ",immunostaining
R5117 T18186 T18184 cc and,immunostaining
R5118 T18187 T18184 conj immunoblots,immunostaining
R5119 T18189 T18190 nsubjpass Antisera,generated
R5120 T18191 T18189 prep against,Antisera
R5121 T18192 T18193 det the,sequence
R5122 T18193 T18191 pobj sequence,against
R5123 T18194 T18193 amod human,sequence
R5124 T18195 T18196 npadvmod C,terminal
R5125 T18196 T18193 amod terminal,sequence
R5126 T18197 T18196 punct -,terminal
R5127 T18198 T18193 compound TIF1γ,sequence
R5128 T18199 T18193 appos RRKRLKSDERPVHIK,sequence
R5129 T18200 T18190 auxpass was,generated
R5130 T18201 T18190 prep in,generated
R5131 T18202 T18201 pobj rabbits,in
R5132 T18203 T18204 punct (,Synthesis
R5133 T18204 T18190 parataxis Synthesis,generated
R5134 T18205 T18204 compound Genemed,Synthesis
R5135 T18206 T18204 punct ", ",Synthesis
R5136 T18207 T18208 compound South,Francisco
R5137 T18208 T18204 npadvmod Francisco,Synthesis
R5138 T18209 T18208 compound San,Francisco
R5139 T18210 T18204 punct ", ",Synthesis
R5140 T18211 T18204 npadvmod California,Synthesis
R5141 T18212 T18204 punct ", ",Synthesis
R5142 T18213 T18214 compound United,States
R5143 T18214 T18204 npadvmod States,Synthesis
R5144 T18215 T18204 punct ),Synthesis
R5145 T18216 T18190 cc and,generated
R5146 T18217 T18218 dep affinity,purified
R5147 T18218 T18190 conj purified,generated
R5148 T18219 T18190 punct .,generated
R5149 T18221 T18222 nmod Mouse,fibroblasts
R5150 T18222 T18224 nsubjpass fibroblasts,immunostained
R5151 T18223 T18222 amod embryonic,fibroblasts
R5152 T18225 T18222 acl grown,fibroblasts
R5153 T18226 T18225 prep on,grown
R5154 T18227 T18226 pobj coverslips,on
R5155 T18228 T18224 auxpass were,immunostained
R5156 T18229 T18224 prep with,immunostained
R5157 T18230 T18231 nmod HP1α,antisera
R5158 T18231 T18229 pobj antisera,with
R5159 T18232 T18233 punct (,Chemicon
R5160 T18233 T18230 parataxis Chemicon,HP1α
R5161 T18234 T18233 punct ", ",Chemicon
R5162 T18235 T18233 npadvmod Temecula,Chemicon
R5163 T18236 T18233 punct ", ",Chemicon
R5164 T18237 T18233 npadvmod California,Chemicon
R5165 T18238 T18233 punct ", ",Chemicon
R5166 T18239 T18240 compound United,States
R5167 T18240 T18233 npadvmod States,Chemicon
R5168 T18241 T18233 punct ),Chemicon
R5169 T18242 T18230 cc and,HP1α
R5170 T18243 T18230 conj Tif1γ,HP1α
R5171 T18244 T18224 advmod simultaneously,immunostained
R5172 T18245 T18224 punct .,immunostained
R5173 T18247 T18248 prep In,fixed
R5174 T18249 T18247 pobj brief,In
R5175 T18250 T18248 punct ", ",fixed
R5176 T18251 T18248 nsubjpass cells,fixed
R5177 T18252 T18248 auxpass were,fixed
R5178 T18253 T18248 prep in,fixed
R5179 T18254 T18255 nummod 4,%
R5180 T18255 T18256 compound %,paraformaldehyde
R5181 T18256 T18253 pobj paraformaldehyde,in
R5182 T18257 T18248 prep for,fixed
R5183 T18258 T18259 nummod 5,min
R5184 T18259 T18257 pobj min,for
R5185 T18260 T18248 punct ", ",fixed
R5186 T18261 T18248 conj washed,fixed
R5187 T18262 T18261 prep with,washed
R5188 T18263 T18264 npadvmod phosphate,buffered
R5189 T18264 T18266 amod buffered,saline
R5190 T18265 T18264 punct -,buffered
R5191 T18266 T18262 pobj saline,with
R5192 T18267 T18261 punct ", ",washed
R5193 T18268 T18261 cc and,washed
R5194 T18269 T18261 conj blocked,washed
R5195 T18270 T18269 prep with,blocked
R5196 T18271 T18272 nummod 5,%
R5197 T18272 T18273 compound %,serum
R5198 T18273 T18270 pobj serum,with
R5199 T18274 T18275 punct (,PBST
R5200 T18275 T18273 parataxis PBST,serum
R5201 T18276 T18275 punct ),PBST
R5202 T18277 T18269 prep for,blocked
R5203 T18278 T18279 nummod 30,min
R5204 T18279 T18277 pobj min,for
R5205 T18280 T18248 punct .,fixed
R5206 T18282 T18283 prep After,washed
R5207 T18284 T18282 pobj incubation,After
R5208 T18285 T18284 prep with,incubation
R5209 T18286 T18287 det the,antibodies
R5210 T18287 T18285 pobj antibodies,with
R5211 T18288 T18287 amod primary,antibodies
R5212 T18289 T18290 punct (,min
R5213 T18290 T18284 parataxis min,incubation
R5214 T18291 T18290 dep PBST,min
R5215 T18292 T18290 punct ", ",min
R5216 T18293 T18290 nummod 60,min
R5217 T18294 T18290 punct ),min
R5218 T18295 T18283 nsubj cells,washed
R5219 T18296 T18283 aux were,washed
R5220 T18297 T18298 nummod three,times
R5221 T18298 T18283 npadvmod times,washed
R5222 T18299 T18283 prep with,washed
R5223 T18300 T18299 pobj PBST,with
R5224 T18301 T18283 cc and,washed
R5225 T18302 T18283 conj incubated,washed
R5226 T18303 T18302 prep with,incubated
R5227 T18304 T18305 amod secondary,antibodies
R5228 T18305 T18303 pobj antibodies,with
R5229 T18306 T18307 punct (,Laboratory
R5230 T18307 T18305 parataxis Laboratory,antibodies
R5231 T18308 T18307 compound Jackson,Laboratory
R5232 T18309 T18307 punct ", ",Laboratory
R5233 T18310 T18311 compound Bar,Harbor
R5234 T18311 T18307 npadvmod Harbor,Laboratory
R5235 T18312 T18307 punct ", ",Laboratory
R5236 T18313 T18307 npadvmod Maine,Laboratory
R5237 T18314 T18307 punct ", ",Laboratory
R5238 T18315 T18316 compound United,States
R5239 T18316 T18307 npadvmod States,Laboratory
R5240 T18317 T18307 punct ),Laboratory
R5241 T18318 T18283 advcl followed,washed
R5242 T18319 T18318 agent by,followed
R5243 T18320 T18321 nummod three,washes
R5244 T18321 T18319 pobj washes,by
R5245 T18322 T18321 prep in,washes
R5246 T18323 T18322 pobj PBST,in
R5247 T18324 T18283 punct .,washed
R5248 T18326 T18327 nsubjpass Cells,embedded
R5249 T18328 T18327 auxpass were,embedded
R5250 T18329 T18327 prep with,embedded
R5251 T18330 T18331 compound Vectashield,DAPI
R5252 T18331 T18329 pobj DAPI,with
R5253 T18332 T18331 punct /,DAPI
R5254 T18333 T18327 cc and,embedded
R5255 T18334 T18327 conj analyzed,embedded
R5256 T18335 T18334 advcl using,analyzed
R5257 T18336 T18337 det an,microscope
R5258 T18337 T18335 dobj microscope,using
R5259 T18338 T18337 nmod Axioplan,microscope
R5260 T18339 T18338 nummod 2,Axioplan
R5261 T18340 T18341 punct (,Zeiss
R5262 T18341 T18337 parataxis Zeiss,microscope
R5263 T18342 T18341 punct ", ",Zeiss
R5264 T18343 T18341 npadvmod Jena,Zeiss
R5265 T18344 T18341 punct ", ",Zeiss
R5266 T18345 T18341 npadvmod Germany,Zeiss
R5267 T18346 T18341 punct ),Zeiss
R5268 T18347 T18327 punct .,embedded
R5269 T18349 T18350 amod Digital,images
R5270 T18350 T18351 nsubjpass images,processed
R5271 T18352 T18351 auxpass were,processed
R5272 T18353 T18351 advcl using,processed
R5273 T18354 T18355 det the,software
R5274 T18355 T18353 dobj software,using
R5275 T18356 T18355 nmod Volocity,software
R5276 T18357 T18356 nummod 1.0,Volocity
R5277 T18358 T18359 punct (,Improvision
R5278 T18359 T18355 parataxis Improvision,software
R5279 T18360 T18359 punct ", ",Improvision
R5280 T18361 T18359 npadvmod Lexington,Improvision
R5281 T18362 T18359 punct ", ",Improvision
R5282 T18363 T18359 npadvmod Massachusetts,Improvision
R5283 T18364 T18359 punct ", ",Improvision
R5284 T18365 T18366 compound United,States
R5285 T18366 T18359 npadvmod States,Improvision
R5286 T18367 T18359 punct ),Improvision
R5287 T18368 T18351 punct .,processed
R5288 T18370 T18371 compound G1E,differentiation
R5289 T18371 T18373 compound differentiation,experiments
R5290 T18372 T18371 compound cell,differentiation
R5291 T18373 T18374 nsubjpass experiments,performed
R5292 T18375 T18374 auxpass were,performed
R5293 T18376 T18374 advmod essentially,performed
R5294 T18377 T18378 mark as,described
R5295 T18378 T18374 advcl described,performed
R5296 T18379 T18380 punct (,Weiss
R5297 T18380 T18374 meta Weiss,performed
R5298 T18381 T18380 nmod et,Weiss
R5299 T18382 T18380 nmod al.,Weiss
R5300 T18383 T18380 nummod 1997,Weiss
R5301 T18384 T18380 punct ),Weiss
R5302 T18385 T18374 punct .,performed
R136 T803 T794 conj rescues,encode
R499 T2994 T2990 punct ),Weinstein
R500 T2995 T2984 punct .,named
R843 T3359 T3358 nsubjpass Tif1β,required
R844 T3360 T3358 auxpass is,required
R846 T3361 T3358 prep for,required
R847 T3362 T3363 compound postimplantation,embryogenesis
R1679 T8144 T8143 auxpass was,hybridized
R2026 T8515 T8514 prep of,each
R2027 T8516 T8517 det the,alleles
R2372 T8888 T8886 amod related,TIF1
R2374 T8889 T8880 prep from,amplify
R2375 T8890 T8891 amod embryonic,RNA