PMC:4996407 / 14637-15385 JSONTXT

{"project":"2_test","denotations":[{"id":"27600246-19795455-69478672","span":{"begin":298,"end":300},"obj":"19795455"}],"text":"2.3.1. Blood Collection, RNA Isolation and RNA Quality Control\nPeripheral whole blood was collected from patients in EDTA Vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ, USA) and PAXgene tubes (PreAnalytix, Hombrechtikon, Switzerland). Whole blood RNA was isolated as described previously [20]. Isolated RNA was checked by using 2100 Bioanalyzer RNA 6000 Nano Chips (Agilent Technologies, Santa Clara, CA, USA). Samples were excluded from microarray analysis that did not meet the following quality criteria: RIN ≥ 7.0; 28S:18S rRNA ≥ 1.0. RNA quantity was determined by absorbance at 260 nm in a DU800 Spectrophotometer (Beckman Coulter, Brea, CA, USA) or a NanoDrop 2000c UV-Vis Spectrophotometer (Thermo Scientific, Wilmington, DE, USA)."}