PMC:4996403 / 3922-5423 JSONTXT

{"project":"2_test","denotations":[{"id":"27600234-9675914-69479874","span":{"begin":263,"end":264},"obj":"9675914"},{"id":"27600234-9408757-69479875","span":{"begin":265,"end":266},"obj":"9408757"},{"id":"27600234-9771718-69479876","span":{"begin":267,"end":268},"obj":"9771718"},{"id":"27600234-9675914-69479877","span":{"begin":406,"end":407},"obj":"9675914"},{"id":"27600234-14500357-69479878","span":{"begin":606,"end":607},"obj":"14500357"},{"id":"27600234-14506695-69479879","span":{"begin":1158,"end":1160},"obj":"14506695"},{"id":"27600234-14723794-69479880","span":{"begin":1161,"end":1163},"obj":"14723794"},{"id":"27600234-19350549-69479881","span":{"begin":1164,"end":1166},"obj":"19350549"},{"id":"27600234-22994622-69479882","span":{"begin":1167,"end":1169},"obj":"22994622"},{"id":"27600234-10084113-69479883","span":{"begin":1217,"end":1219},"obj":"10084113"}],"text":"2.1. Microarray Devices (DNA Chip Synthesis)\n\n2.1.1. In Situ Synthesized Type\nThe methods for preparing DNA chips combine photochemistry and photolithography with solid-phase DNA synthesis chemistry to generate a high-density oligonucleotide probe array in situ [6,7,8]. These two-dimensional arrays containing hundreds or thousands of oligonucleotide probes provide a powerful DNA sequence analysis tool [6]. For example, this method is now used to produce the high-density gene chip probe arrays, which are used for the detection and analysis of point mutations and SNPs and for gene expression studies [9]. The change of the carried probe number in expression array by Affymetrix shows Table 1.\nmicroarrays-04-00454-t001_Table 1 Table 1 The change Probe/GeneChip number in expression arrays by Affymetrix.\n\n2.1.2. Spotting Type\nMany microarray spotting technologies and techniques have been successfully developed. DNA chips can be produced using the spot method, in which cDNAs are selectively deposited on specific positions on a glass slide using a spotter. Two of the more important spotting techniques used are the pin-based fluid transfer systems [10,11,12,13] and the piezo-based inkjet dispenser systems [14]. Table 2 shows the change of the coverage of genes and transcripts in expression array by Agilent. The microarray devices have rapidly developed.\nmicroarrays-04-00454-t002_Table 2 Table 2 The change of the coverage of the genes and transcripts in expression array by Agilent.\n\n3."}