PMC:4996396 / 13730-14568 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4996396","sourcedb":"PMC","sourceid":"4996396","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4996396","text":"2.2. Human IgG Isolation\nHuman immunoglobulin G (IgG) was isolated from plasma samples using the Melon®Gel Spin Plate Kit for IgG Screening (Thermo Scientific, Rockford, IL, USA) following the manufacturer’s instructions. IgG samples were stored over night at 4 °C until further processing. Concentration of purified IgG was measured spectrophotometrically at 280 nm on an Epoch Microplate Spectrophotometer (Biotek, Winooski, VT, USA). As blank sample Melon Gel Purification Buffer was used. Samples were measured in duplicates and arithmetic average was calculated. Pre-casted NuPAGE® 4%–12% Bis-Tris polyacrylamide gels (Novex®, Carlsbad, CA, USA) were used to verify IgG purity. The strongest band was observed at 150 kDa, which corresponds to the molecular weight of undegraded human IgG [44]. Integrity of all samples was confirmed.","divisions":[{"label":"Title","span":{"begin":0,"end":24}}],"tracks":[{"project":"2_test","denotations":[{"id":"27600218-11406581-69481427","span":{"begin":794,"end":796},"obj":"11406581"}],"attributes":[{"subj":"27600218-11406581-69481427","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93e0ec","default":true}]}]}}