PMC:4996394 / 12024-13478 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4996394","sourcedb":"PMC","sourceid":"4996394","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4996394","text":"3.4. Impact of the Finding\nResearchers working with modern TMA technologies have a growing interest in the possible application of TMA instrumentation for purposes other than TMA block construction. Especially, a combined digital pathology/TMA approach is ideal for assessing issues of heterogeneity and allows very precisely annotated histological regions to be transferred either to TMAs or to tubes for storage and/or analysis of nucleic acids. With or without a digital pathology platform, neighboring punches side-by-side for TMA construction and simultaneous molecular analysis is clearly advantageous in the era of molecular pathology.\nThis study was motivated by the growing concerns over cross-contamination between samples and appears to be the first study to date to address this issue. Using two different PCR methods and alternating between positive/mutated and negative/wild-type samples, our findings underline no evidence of transfer of material from one tissue block to the next.\nFigure 3 Electropherograms used to evaluate the presence of mycobacterium in donor blocks from a previously confirmed positive and negative tissue sample. (A) positive control and (B) negative control. Tissue samples were alternated in the tissue arrayer three times leading to the following results: (C,E,G) mycobacterium tuberculosis samples and (D,F,H) mycobacterium tuberculosis negative samples. No evidence for cross-contamination between samples.\n\n3","divisions":[{"label":"Title","span":{"begin":0,"end":26}},{"label":"Figure caption","span":{"begin":997,"end":1453}}],"tracks":[]}