PMC:4996393 / 27363-29269
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4996393","sourcedb":"PMC","sourceid":"4996393","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4996393","text":"5. Outlook \nRPPA has been developed for quantitative protein measurements of protein levels and to identify post-translationally modified forms in signaling pathways suitable for large scale multiplexed studies. Recent and ongoing research highlights the potential of RPPA for personalized medicine. The information provided by RPPA is already being used in clinical studies to characterize tumors and to select the optimal treatment for each patient. For routine clinical use in the future there are at least two major challenges: (i) The limitation of available, validated and highly specific antibodies [24] and (ii) the poor quality of starting material that can affect the results of the molecular assay [16]. Furthermore, selection of a tumor sample for RPPA and other molecular analysis shall be performed by a pathologist to avoid non-tumorous areas or areas with necrosis. As tumor samples may be heterogeneous and composed of different cell types besides tumor cells (e.g., stromal or inflammatory cells) estimation of the tumor cell content by a pathologist is important. For continued exchange of information with regard to RPPA, an annual Global RPPA Workshop is held which started in 2011. To move RPPA forward to the clinic the “RPPA society” was founded in 2014 during the 4th Global RPPA Workshop held in Paris with the idea of proceeding in sharing information about RPPA guidelines, principles, and improving RPPA technologies. To solve the problem of antibody limitation an antibodypedia-website was already set up which is an open-access resource where already evaluated antibodies are listed that can be used to detect proteins of the human proteome (http://www.antibodypedia.com). However, still, highly specific antibodies against most proteins are missing. Consequently, although new protein biomarkers are continuously identified, their quantitative analysis remains a challenge. ","divisions":[{"label":"Title","span":{"begin":0,"end":11}}],"tracks":[{"project":"2_test","denotations":[{"id":"27600215-20974554-69479544","span":{"begin":607,"end":609},"obj":"20974554"},{"id":"27600215-23913224-69479545","span":{"begin":710,"end":712},"obj":"23913224"}],"attributes":[{"subj":"27600215-20974554-69479544","pred":"source","obj":"2_test"},{"subj":"27600215-23913224-69479545","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#d193ec","default":true}]}]}}