PMC:4996384 / 4804-5995 JSONTXT

{"project":"2_test","denotations":[{"id":"27600212-19562201-69481637","span":{"begin":1069,"end":1071},"obj":"19562201"}],"text":"2.1. Reagents and Chemicals\nGold nanoparticles (AuNPs) with a diameter of 20 nm were purchased from BBI Solutions (Cardiff, UK). Adenosine, guanosine, HO-(CH2)11-PEG-SH (MW 336.5Da) named PEG300, Bis-(p-sulfonatophenyl) phenylphosphine dihydrate dipotassium (BSPP) and all buffer reagents were purchased from Sigma‑Aldrich (Saint Quentin Fallavier, France). CH3O-PEG-SH (MW 2000 Da) named PEG2000, was purchased from Rapp Polymere GmbH (Tübingen, Germany). Different concentrations of adenosine and 1 µM guanosine were prepared in the SPR running buffer (HEPES 10 mM, MgCl2 5 mM, NaCl 150 mM and 0.005% Tween20, pH 7.4). Ultrapure water (18.2 MΩ·cm) was used throughout.\nThe oligonucleotides were purchased from Eurogentec (Angers, France) with a thiol modification at their 5' position and a thymine spacer (five or ten thymine nucleotide) before the sequence of interest. Sequences are listed in Table 1. APT4 and APT8 correspond to the full adenosine aptamers and Split‑APT4, Split‑APT8 and Split-APT are their split sequences, designed according to the literature [17]. CN8 sequence was used as a negative control.\nmicroarrays-04-00041-t001_Table 1 Table 1 Oligonucleotide sequences.\n\n2"}