PMC:4996382 / 9833-11136 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4996382","sourcedb":"PMC","sourceid":"4996382","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4996382","text":"2.4. Virtual Hybridization by Direct and Extended Methods\nVH utilizes two methods: (a) the direct method identifies all the sites where virtual hybridization with the genome might potentially occur and then, using the cut-off value, identifies the sites with high probability for heteroduplex formation (Figure 1); (b) the extended method also identifies the sites where virtual hybridization between the genome and the UFC-13 might potentially occur, but signals corresponding to non-conserved sequences are discarded to leave only those corresponding to virtual hybridization with homologous sites. This method increases the alignment between the probe and the site in the genome, by adding four nucleotides onto the right and left ends of the probe (4 + 13 + 4), with the sequences present in the target DNA, allowing only one difference between them, thus ensuring that their coincidence is not random, and can, therefore, be considered as conserved (Figure 2).\nFigure 1 Virtual hybridization (VH)—Direct method. Illustrative example of four probes being used to predict the sites where virtual hybridization with the target genome (represented by the blue line) might potentially occur. Then, VH software estimates the ΔG° value for each of the probes and identifies the high-probability sites.\n\n2","divisions":[{"label":"Title","span":{"begin":0,"end":57}},{"label":"Figure caption","span":{"begin":966,"end":1302}}],"tracks":[]}