PMC:4996382 / 7496-8325 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4996382","sourcedb":"PMC","sourceid":"4996382","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4996382","text":"2.2. Universal Fingerprinting Chip (UFC-13)\nAn oligonucleotide probe is a short piece of single-stranded DNA that is complementary to the target to be measured on the microarray. A set of 15,264 13-mer oligonucleotide sequences constitutes the UFC-13 chip that was used for hybridizing with all the Bacillus anthracis genomes strains selected. The UFC-13 includes: (a) a 35%–65% G + C content; (b) between-sequences differences have been maximized such that all sequences differ in at least three bases from each other; and (c) the sequences Tm ranges between 52 °C and 68 °C. Bacillus anthracis has an average of 5.5 Mb, and the size of the 13-mer probes is suitable for bacterial genomes [13].\nmicroarrays-04-00084-t001_Table 1 Table 1 Bacillus anthracis genomes used in this study. * Related GenBank Project (Accession).\n\n2","divisions":[{"label":"Title","span":{"begin":0,"end":43}},{"label":"Table caption","span":{"begin":696,"end":828}}],"tracks":[{"project":"2_test","denotations":[{"id":"27600214-21906634-69478337","span":{"begin":691,"end":693},"obj":"21906634"}],"attributes":[{"subj":"27600214-21906634-69478337","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#e893ec","default":true}]}]}}