PMC:4925210 / 23552-24455 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4925210","sourcedb":"PMC","sourceid":"4925210","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4925210","text":"Cell lines\nHEK293, HEK293T, and CHO-K1 cell lines were obtained as authenticated stocks from ATCC. Wild type, Pkd1−/−, and Pkd2−/− cells were obtained from The Baltimore PKD Center (http://www.baltimorepkdcenter.org/). Drosophila Schneider S2 cells were purchased from Invitrogen. The HEK293 cell line has been misidentified according to ICLAC, but it was only used here to overexpress PKD1 fragments as Fc fusions and myc-tagged WNT5A due to its high transfectability. All cells lines were tested negative for mycoplasma using PCR and DAPI staining. HEK293 or HEK293T cells were maintained in DMEM supplemented with 10% FBS. CHO-K1 were grown in Ham's F-12/DMEM with 10% FBS. Pkd1−/− and Pkd2−/− mouse embryonic fibroblasts (MEFs) and wild type littermate type control cells were cultured in DMEM supplemented with 10% heat inactivated FBS, 1% non-essential amino acids, and 1% penicillin-streptomycin.","divisions":[{"label":"Title","span":{"begin":0,"end":10}}],"tracks":[]}