PMC:4845325 / 52085-52725 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4845325","sourcedb":"PMC","sourceid":"4845325","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4845325","text":"Human NTERA cells expressing eGFP under the DCX promoter were maintained in DMEM media containing 10 % FBS. To induce differentiation, cells were plated in 96 well plates. One day after seeding, 10 μM of retinoic acid and designated concentrations of recombinant carrier-free human GDF3 (R\u0026D Systems, Minneapolis, MN; at 0, 10, 50, or 150 ng/mL) were added to the culture media of each corresponding NTERA treatment well. Cells were maintained under these conditions for 2 weeks, during which media was replaced every 3 days. Cells were then cultured for an additional 2 weeks with continued GDF3 treatment, in the absence of retinoic acid.","tracks":[]}